Guanine nucleotide binding proteins in opioid-dependent patients.
Guanine nucleotide binding (G) protein levels and functioning in the platelets of 19 methadone-maintained patients were compared to age and sex matched, normal controls. We found that in the methadone patients, G alpha s-levels were significantly higher, while the levels of G alpha i 1/2 and pertussis toxin catalyzed [32P]ADP ribosylation were significantly lower compared to control subjects in platelet membranes. We have further found that when all three of these biochemical indicators were combined in a discriminant function analysis, 79% of the methadone patients were correctly classified and 83% of the controls were correctly classified.
Manji
H
H
Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, Michigan, USA.
Chen
G
G
Potter
W
W
Kosten
T R
TR
D000246:Adenosine Diphosphate Ribose / Q000097:blood; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000097:blood; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000097:blood; D019204:GTP-Binding Proteins / Q000097:blood*; D006801:Humans; D008297:Male; D008691:Methadone / Q000627:therapeutic use*; D009293:Opioid-Related Disorders / Q000097:blood* / Q000175:diagnosis / Q000534:rehabilitation; D012016:Reference Values; D012680:Sensitivity and Specificity
D000246:Adenosine Diphosphate Ribose; D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D019205:GTP-Binding Protein alpha Subunits, Gs; D008691:Methadone