Functional and biochemical evaluation of the preserved lung in a rat model.
Lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) activities and total protein concentrations were examined in the postrinsing solutions from rat lungs preserved in phosphate-buffered saline (PBS), Euro-Collins (E-C) solution, or low-potassium E-C solution for 8 h at 4 degrees C, 10 degrees C, or 20 degrees C. The LDH and AST activities were higher when the organs were preserved in PBS for 8 h at 20 degrees C than at 4 degrees C or 10 degrees C, while the total protein concentration did not differ according to the temperature or solution. The activities were also higher when the lungs were preserved in Euro-Collins (E-C) solution than in PBS or the low-potassium E-C solution. On examining pulmonary functions utilizing an ex vivo reperfusion model, the lungs preserved at 20 degrees C showed poorer gas exchange than those preserved at 4 degrees C or 10 degrees C. Moreover, the organs preserved in E-C solution showed poorer function than those preserved in any other solution. These findings suggest that some enzymatic activities in the postrinsing solution could be indicators of lung viability after preservation.
Hanagiri
T
T
Second Department of Surgery, School of Medicine, Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu, Japan.
Igisu
H
H
Matsumura
H
H
Yasumoto
K
K
D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D006982:Hypertonic Solutions; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000201:enzymology* / Q000502:physiology*; D009926:Organ Preservation / Q000379:methods; D010710:Phosphates; D011188:Potassium; D011506:Proteins / Q000378:metabolism; D011659:Pulmonary Gas Exchange; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D014022:Tissue Survival
C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D010710:Phosphates; D011506:Proteins; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D011188:Potassium