Induction of murine CD5 expression by v-H-ras.
The murine CD5 surface antigen is a frequent marker on B-lineage cell lines produced from bone marrow infected with retroviruses expressing v-H-ras. Since CD5+ B cells cannot be detected in adult murine bone marrow, either the viral targets of transformation are a minor contaminating population of CD5+ B-lineage cells or the v-H-ras oncogene is inducing the expression of CD5 on B-lineage cells not previously expressing this marker. We have found that v-H-ras can induce the expression of CD5 on two CD5+ pre-B-cell lines established from murine bone marrow. This induction correlates with increased steady-state levels of CD5 mRNA. These results present the possibility that CD5 expression may be modulated by specific signalling as well as early lineage commitment.
Weichert
T R
TR
Department of Microbiology, Michigan State University, East Lansing 48824-1101, USA.
Schwartz
R C
RC
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D015152:Blotting, Northern; D001853:Bone Marrow / Q000276:immunology; D018956:CD5 Antigens / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002469:Cell Separation; D005434:Flow Cytometry; D015870:Gene Expression; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D018631:ras Proteins / Q000276:immunology*
D018956:CD5 Antigens; D012333:RNA, Messenger; D018631:ras Proteins