Direct detection of equine herpesvirus DNA in tissues of aborted equine fetuses.
Restriction endonuclease analysis of equine herpesviruses 1 (EHV-1) and 4 has been investigated using cultured cells infected with these viruses. The DNA cleavage patterns of these viruses were observed in the intracellular DNA after digestion with Eco RI and electrophoresis. This procedure was applied to the diagnosis of equine herpesvirus infection in aborted equine fetuses. The characteristic Eco RI restriction pattern of EHV-1 DNA was directly detectable in the emulsion of lungs collected from aborted equine fetuses.
Ishiyama
T
T
Hokkaido Branch, National Institute of Animal Health, Japan.
Nishimori
T
T
Kato
M
M
Yamada
H
H
Sato
K
K
Sentsui
H
H
D000034:Abortion, Veterinary / Q000209:etiology; D000818:Animals; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D015246:Deoxyribonuclease EcoRI / Q000494:pharmacology; D004586:Electrophoresis / Q000379:methods / Q000662:veterinary; D005260:Female; D005333:Fetus / Q000737:chemistry* / Q000473:pathology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D006734:Horse Diseases / Q000175:diagnosis* / Q000473:pathology; D006736:Horses; D008168:Lung / Q000737:chemistry* / Q000196:embryology / Q000473:pathology; D011247:Pregnancy
D004279:DNA, Viral; D015246:Deoxyribonuclease EcoRI