title: PCR site-directed mutagenesis using Pyrococcus sp GB-D polymerase coupled to a rapid screening procedure. Application to a beta-glucanase gene.
authors.affiliation: Instituto de Biologia Fundemental, University Autónoma de Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Pons
authors.forename: A
authors.initials: A
authors.lastname: Planas
authors.forename: M
authors.initials: M
authors.lastname: Juncosa
authors.forename: E
authors.initials: E
authors.lastname: Querol
mesh_terms: D001105:Archaea / Q000201:enzymology; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D004274:DNA, Recombinant / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D007202:Indicators and Reagents; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant; D007202:Indicators and Reagents; D011994:Recombinant Proteins; C105117:Pyrococcus sp GB-D DNA polymerase; D004259:DNA-Directed DNA Polymerase; D006026:Glycoside Hydrolases; C028020:licheninase