Effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on biomaterial-associated staphylococcal infection in mice.
Staphylococcal infections are a major complication in the usage of biomaterials. Different modifications of polymers have been made to reduce the incidence of such infections. We studied the effects of modifying heparinized polyethylene (H-PE) with mouse recombinant granulocyte-macrophage stimulating factor (rGM-CSF). The elimination of staphylococci (Staphylococcus aureus, S. epidermidis) from the peritoneum of mice implanted with rGM-CSF-coated H-PE was slightly more effective than the elimination of the bacteria from the peritoneum of animals implanted with uncoated H-PE. Most interestingly, the number of staphylococci present in the biofilms covering rGM-CSF-coated implants were significantly lower than the number of bacteria detected on the surface of H-PE not coated with rGM-CSF. In vitro, rGM-CSF restored the anti-bacterial potency of the phagocytes, which had been reduced by surface contact with H-PE. The results suggest that modification of biomaterials with rGM-CSF could be one way of preventing staphylococcal infections; especially in neutropenic disorders, which constitute the highest risk factor for foreign body-associated infections.
Rózalska
B
B
Department of Infectious Biology, University of Lódź, Banacha, Poland.
Ljungh
A
A
Paziak-Domańska
B
B
Rudnicka
W
W
D000818:Animals; D001672:Biocompatible Materials; D018441:Biofilms; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006493:Heparin; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D010537:Peritoneum / Q000382:microbiology; D010587:Phagocytosis; D011095:Polyethylenes; D019736:Prostheses and Implants; D011994:Recombinant Proteins / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000302:isolation & purification
D001672:Biocompatible Materials; D011095:Polyethylenes; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006493:Heparin