Regulation of microtubule dynamics by the neuronal growth-associated protein SCG10.
Dynamic assembly and disassembly of microtubules is essential for cell division, cell movements, and intracellular transport. In the developing nervous system, microtubule dynamics play a fundamental role during neurite outgrowth, elongation, and branching, but the molecular mechanisms involved are unknown. SCG10 is a neuron-specific protein that is membrane-associated and highly enriched in growth cones. Here we show that SCG10 binds to microtubules, inhibits their assembly, and can induce microtubule disassembly. We also show that SCG10 overexpression enhances neurite outgrowth in a stably transfected neuronal cell line. These data identify SCG10 as a key regulator of neurite extension through regulation of microtubule instability.
Riederer
B M
BM
Institut de Biologie Cellulaire et de Morphologie, Lausanne, Switzerland.
Pellier
V
V
Antonsson
B
B
Di Paolo
G
G
Stimpson
S A
SA
Lütjens
R
R
Catsicas
S
S
Grenningloh
G
G
D000818:Animals; D002352:Carrier Proteins; D002454:Cell Differentiation; D019084:Fluorescent Antibody Technique, Indirect; D008027:Light; D046911:Macromolecular Substances; D008565:Membrane Proteins; D008868:Microtubule Proteins; D008870:Microtubules / Q000502:physiology*; D009414:Nerve Growth Factors / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology*; D016501:Neurites / Q000648:ultrastructure; D016716:PC12 Cells; D051381:Rats; D012542:Scattering, Radiation; D013552:Swine; D014404:Tubulin / Q000502:physiology*
D002352:Carrier Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D008868:Microtubule Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; C512848:Stmn2 protein, rat; D014404:Tubulin