HIV-1 cDNA integration: requirement of HMG I(Y) protein for function of preintegration complexes in vitro.
We present data indicating that a host protein is important for function of HIV-1 preintegration complexes (PICs) in vitro. PICs partially purified from infected cells were subjected to gel filtration in 600 mM KCl, which removed a factor required for integration without fully disrupting PICs. Addition of an extract from uninfected cells restored activity. Fractionation of the complementing activity yielded HMG I(Y), a nonhistone chromosomal protein important for transcriptional control and chromosomal architecture. Complementing activity could be isolated from PICs, and activity could be depleted from such fractions with an antibody against HMG I(Y). Recombinant HMG I(Y) also complemented salt-stripped complexes. The finding that a host protein is required for integration by HIV PICs parallels findings in several well-studied transposition and site-specific recombination systems.
Farnet
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CM
Salk Institute for Biological Studies, La Jolla, California 92024, USA.
Bushman
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D000918:Antibody Specificity; D002457:Cell Extracts / Q000502:physiology; D002458:Cell Fractionation; D002460:Cell Line / Q000737:chemistry / Q000502:physiology / Q000821:virology; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000502:physiology; D005816:Genetic Complementation Test; D015497:HIV-1 / Q000502:physiology*; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D013601:T-Lymphocytes / Q000737:chemistry / Q000502:physiology / Q000821:virology; D016662:Virus Integration
D002457:Cell Extracts; D018076:DNA, Complementary; D004279:DNA, Viral; D006609:High Mobility Group Proteins; D025741:HMGA1a Protein