14-3-3 zeta protein binds to the carboxyl half of mouse wee1 kinase.
To identify proteins which bind to mouse wee1 kinase, the yeast "two-hybrid" system was used with a mouse cDNA library. Using the carboxyl half of weel kinase, the 14-3-3 zeta protein was isolated. Recombinant 14-3-3 zeta was demonstrated to bind to wee1 kinase in vitro. The wee1 kinase phosphorylated by cdc2 kinase also bound to 14-3-3 zeta protein. When both wee1 kinase and 14-3-3 zeta were transfected into COS-1 cells, they formed a complex in a cell. The sequence of wee1 kinase necessary for the binding was tested by a two hybrid system expressing different lengths of peptides derived from wee1 kinase. Both the entire kinase domain and a sequence in the carboxyl terminus was thought to be necessary for the binding. The function of 14-3-3 zeta protein remained to be elucidated in relation to the regulation of G2 to M phase transition through wee1 kinase.
Honda
R
R
School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Ohba
Y
Y
Yasuda
H
H
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D016203:CDC2 Protein Kinase / Q000302:isolation & purification / Q000378:metabolism; D019556:COS Cells; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism; D055503:Protein Multimerization; D011505:Protein-Tyrosine Kinases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera; D014162:Transfection; D020798:Two-Hybrid System Techniques; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D011506:Proteins; D011994:Recombinant Proteins; D029702:Schizosaccharomyces pombe Proteins; D014446:Tyrosine 3-Monooxygenase; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; C499348:Wee1 protein, mouse; D016203:CDC2 Protein Kinase