title: Virus-specific IgD in acute viral infection of mice.
abstract: In phylogenetically diverse species with the help of T lymphocytes or soluble factors, viral infections induce the Ag-specific B lymphocytes to proliferate and terminally differentiate into IgM, IgG, IgA, IgD, or IgE Ab-secreting cells. Based on previous studies searching for IgD, it was inferred that serum IgD in the mouse is nearly undetectable, although in other species, e.g., humans, IgD is a measurable component of serum Ig. More recently, new information has been obtained indicating that IgD is secreted in minute quantities during normal B cell differentiation. We observed that IgD is secreted in significantly increased quantities in mice undergoing an acute infection with lymphocytic choriomeningitis virus or vesicular stomatitis virus compared with uninfected animals. A substantial fraction of the observed IgD was found to be virus specific. Using a solid-phase immunoenzymatic technique, virus-specific IgD Ab-forming cells were detected in the spleen; their numerical increase correlated with the level of secreted antiviral IgD. In addition, immunohistochemical staining revealed IgD+ plasma cells that occurred with a similar kinetic profile as the virus-specific IgD Ab-forming cells. These findings provide direct evidence that synthesis of IgD is a physiologic event in the mouse. Its precise function in the immune response to pathogens, however, remains to be determined.
authors.affiliation: Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta 30912, USA.
authors.forename: D
authors.initials: D
authors.lastname: Moskophidis
authors.forename: M
authors.initials: M
authors.lastname: Moskophidis
authors.forename: J
authors.initials: J
authors.lastname: Löhler
mesh_terms: D000208:Acute Disease; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D007072:Immunoglobulin D / Q000276:immunology*; D007156:Immunologic Memory; D008216:Lymphocytic Choriomeningitis / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D018353:Rhabdoviridae Infections / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013997:Time Factors; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007072:Immunoglobulin D