title: Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the homodimeric bacterial hemoglobin from Vitreoscilla stercoraria.
abstract: The recombinant homodimeric hemoglobin from the strictly aerobe gram-negative bacterium Vitreoscilla stercoraria has been expressed in Escherichia coli, purified to homogeneity, and crystallized by vapor diffusion techniques, using ammonium sulfate as precipitant. The crystals belong to the monoclinic space group P2(1) and diffract to HIGH resolution. The unit cell parameters are alpha = 62.9, b = 42.5, c = 63.2 A, beta = 106.6 degrees; the asymmetric unit contains the homodimeric hemoglobin, with a volume solvent content of 42%.
authors.affiliation: Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Tarricone
authors.forename: S
authors.initials: S
authors.lastname: Calogero
authors.forename: A
authors.initials: A
authors.lastname: Galizzi
authors.forename: A
authors.initials: A
authors.lastname: Coda
authors.forename: P
authors.initials: P
authors.lastname: Ascenzi
authors.forename: M
authors.initials: M
authors.lastname: Bolognesi
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics; D001420:Bacteria, Aerobic / Q000737:chemistry*; D003001:Cloning, Molecular; D006454:Hemoglobins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D006454:Hemoglobins