Strongylus asini (Nematoda, Strongyloidea): genetic relationships with other Strongylus species determined by ribosomal DNA.
Genomic DNA was isolated from adult Strongylus asini collected from zebra. The second ribosomal transcribed spacer (ITS-2) was amplified and sequenced using polymerase chain reaction (PCR) based techniques. The DNA sequence was compared with previously published data for 3 related Strongylus species. A PCR-linked restriction fragment length polymorphism method allowed the 4 species to be differentiated unequivocally. The ITS-2 sequence of S. asini was found to be more similar to those of S. edentatus (87.1%) and S. equinus (95.3%) than to that of S vulgaris (73.9%). This result confirms that S. Asini and S vulgaris represent separate species and supports the retention of the 4 species within 1 genus.
Hung
G C
GC
Department of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia.
Jacobs
D E
DE
Krecek
R C
RC
Gasser
R B
RB
Chilton
N B
NB
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009276:Namibia; D010527:Perissodactyla / Q000469:parasitology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013019:South Africa; D013323:Strongylus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D004275:DNA, Ribosomal