Visualization of liver sinusoidal endothelial cell repair behavior after preservation by in vitro time-lapse video microscopy.
Sinusoidal endothelial cells are significantly more vulnerable to cold storage and reperfusion than hepatocytes. In this study, a method for assessing the repair behavior of sinusoidal endothelial cells in vitro, after preservation, was investigated. Time-lapse video microscopy analysis was performed and migration rates, division rates, and cell detachment rates were determined. Preservation intervals between 3 and 24 hr and reoxygenation times between 4 and 24 hr were compared. A comparison between sinusoidal endothelial cultures that were stored for 6 hr in University of Wisconsin solution and nonpreserved control cultures was performed. This method allows the investigation of the repair capability of individual cells in vitro. Indications of the kind of preservation/reoxygenation injury that occurs after treatment with several preservation solutions and the resultant repair behavior can be obtained.
Gerlach
J C
JC
Surgical Department, Virchow-Klinikum, Medizinische Fakultat der Humboldt Universitat Berlin, Germany.
Ziemer
R A
RA
Spatkowski
G
G
Zeilinger
K
K
Neuhaus
P
P
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000009:adverse effects; D002465:Cell Movement / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology* / Q000648:ultrastructure; D016031:Liver Transplantation / Q000473:pathology*; D008297:Male; D018715:Microscopy, Video; D008938:Mitosis / Q000187:drug effects; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D013552:Swine
D019188:Organ Preservation Solutions; C071285:hydroxyethyl starch-free University of Wisconsin solution; D010100:Oxygen