Proviral organization and sequence analysis of feline immunodeficiency virus isolated from a Pallas' cat.
The nucleotide sequence and genomic organization have been determined for a highly cytopathic feline immunodeficiency virus (FIV) isolated from a Pallas' cat. The 9747-bp provirus of this virus, FIV-Oma, has typical lentivirus organization with LTRs, gag, pol, and env open reading frames (ORFs), putative vif and rev ORFs, and an ORF similar to ORF2/ORFA of domestic cat FIV isolates. Although the FIV-Oma provirus is 300 to 600 bp longer than other FIV proviruses, these additional bases are distributed throughout the genome. Phylogenetic analysis of a conserved region of the pol gene suggests that FIV-Oma is more closely related to some of the puma and lion lentiviruses than it is to domestic cat FIV isolates; however, many regions of the genome exhibit extensive nucleotide sequence divergence. None of the eight molecular proviral clones isolated from a genomic library are infectious, but we have constructed an infectious, cytopathic clone of FIV-Oma from subcloned and PCR-amplified fragments of these proviral clones. This clone will be useful for identifying the genetic determinants of FIV-Oma's biological activities.
Barr
M C
MC
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
Zou
L
L
Long
F
F
Hoose
W A
WA
Avery
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D004279:DNA, Viral; D016679:Genome, Viral; D016089:Immunodeficiency Virus, Feline / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016086:Lentivirus / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011533:Proviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis*; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D012367:RNA, Viral