Studies of the mouse Rab geranylgeranyl transferase beta subunit: gene structure, expression and regulation.
The mouse Rab geranylgeranyl transferase beta (Rab GGTase beta) catalytic subunit gene was isolated and characterized. This gene (Rabggtb) spans a distance of approx. 7 kb and is organized into eight exons. All the exon/intron junction sequences follow the GT/AG rule. Multiple transcription initiation sites are located within 384 bp upstream from the translation start codon. The 5'-flanking region contains several potential binding sites for transcription factors, but no TATA box is identified in this region. Expression of this gene was detected in all the major organs in adult animals. In mouse embryos, its expression was examined by in situ hybridization. Specific expression of this gene was elevated in mid-gestation stages, particularly developing liver and spinal cord. Northern blot analysis of an embryonic carcinoma cell line P19 showed that the steady-state level of Rabggtb mRNA expression was increased dramatically by cycloheximide (CHX) treatment as early as 2 h, suggesting a role of post-transcriptional regulation of Rab GGTase beta gene expression. Actinomycin D was used to determine the half-life of Rab GGTase beta transcripts CHX treatment resulted in a dramatic increase of the half-life of Rab GGTase beta transcripts, from 8 h to greater than 12 h.
Chinpaisal
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Department of Pharmacology, University of Minnesota, Minneapolis 55455, USA.
Lee
C H
CH
Wei
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LN
D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002384:Catalysis; D003513:Cycloheximide / Q000494:pharmacology; D018076:DNA, Complementary; D003609:Dactinomycin / Q000494:pharmacology; D004622:Embryo, Mammalian; D005091:Exons; D015870:Gene Expression / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014166:Transferases / Q000235:genetics*; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D019384:Nucleic Acid Synthesis Inhibitors; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D003609:Dactinomycin; D003513:Cycloheximide; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C075607:Rab geranylgeranyltransferase