In situ detection of tandem DNA repeat length.
A simple method for scoring short tandem DNA repeats is presented. An oligonucleotide target, containing tandem repeats embedded in a unique sequence, was hybridized to a set of complementary probes, containing tandem repeats known lengths. Single-stranded loops structures formed on duplexes containing a mismatched (different) number of tandem repeats. No loop structure formed on duplexes containing a matched (identical) number of tandem repeats. The matched and mismatched loop structures were enzymatically distinguished and differentially labeled by treatment with S1 nuclease and the Klenow fragment of DNA polymerase.
Yaar
R
R
Center for Advanced Biotechnology, Boston University, MA 02215, USA.
Szafranski
P
P
Cantor
C R
CR
Smith
C L
CL
D001483:Base Sequence; D004256:DNA Polymerase I / Q000378:metabolism; D004277:DNA, Single-Stranded; D017403:In Situ Hybridization / Q000379:methods*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism
D004277:DNA, Single-Stranded; D004256:DNA Polymerase I; D015719:Single-Strand Specific DNA and RNA Endonucleases