title: Normal cellular replication of Sendai virus without the trans-frame, nonstructural V protein.
abstract: The Sendai virus V protein is a nonstructural trans-frame protein in which a highly conserved cys-rich Zn2+-binding domain is fused to the N-terminal half of the P protein via mRNA editing. Using a recently developed system in which infectious virus is recovered from cDNA, we have engineered a virus in which a translation stop codon was placed at the beginning of the V ORF. Translation of the V(stop) mRNA yields a W-like protein, i.e., a protein composed of the N-terminal half of the P protein alone which is naturally expressed at low levels from the P gene. This V-minus but W-augmented virus was found to replicate normally in cell culture and embryonated chicken eggs. The Sendai virus V protein is thus an accessory protein, and the cys-rich Zn2+-binding domain is likely to function in a specialized role during virus propagation.
authors.affiliation: Department of Genetics and Microbiology, University of Geneva School of Medicine, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Delenda
authors.forename: S
authors.initials: S
authors.lastname: Hausmann
authors.forename: D
authors.initials: D
authors.lastname: Garcin
authors.forename: D
authors.initials: D
authors.lastname: Kolakofsky
mesh_terms: D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D006224:Cricetinae; D004285:Dogs; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008253:Macaca mulatta; D016366:Open Reading Frames; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018108:Respirovirus / Q000235:genetics / Q000502:physiology*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins