Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qter.
Mouse Ocp2-rs2 maps to chromosome 11 and encodes an 18.6 kDa peptide abundantly expressed in the organ of Corti. We show that sequences similar to murine Ocp2-rs2 are found on human chromosomes 4p16.2-4p14, 5p13-5q35.2, 7pter-q22, 10 and 12p13-12qter as revealed by Southern blot analyses of human/rodent somatic cell hybrids. A fetal human inner ear cDNA library was screened with a cloned 254 bp PCR product of murine Ocp2-rs2. One of two human cDNA clones (CM1) was sequenced from the 5' end that begins with murine Ocp2-rs2 codon 14 through the stop codon and 258 nucleotides of 3-UTR and was found to have the identical deduced amino acid sequence to Ocp2-rs2. Based on the sequence in the 3'-UTR of CM1, a PCR primer pain was synthesized and used to confirm that a human homologue of Ocp2-rs2, designated OCP2 and expressed in the developing human inner ear, is localized to 5q22-5q35.2. Other OCP2-like sequences located on chromosomes 4p16.2-4p14, 7pter-q22 and 12p13-12qter (but not the chromosome 10 OCP2-like sequence) will PCR amplify the expected size product at a lower annealing temperature using the OCP2 3'-UTR PCR primers indicating that there may be a human OCP2 gene family.
Liang
Y
Y
Graduate Program in Genetics, Michigan State University, East Lansing 48824-1115, USA.
Chen
H
H
Asher
J H
JH
Chang
C C
CC
Friedman
T B
TB
D000818:Animals; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D002881:Chromosomes, Human, Pair 12; D002894:Chromosomes, Human, Pair 4; D002895:Chromosomes, Human, Pair 5; D002897:Chromosomes, Human, Pair 7; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary; D007758:Ear, Inner / Q000196:embryology / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D044786:S-Phase Kinase-Associated Proteins; D014157:Transcription Factors / Q000737:chemistry*
D018076:DNA, Complementary; D044786:S-Phase Kinase-Associated Proteins; C097351:SKP1 protein, human; D014157:Transcription Factors