title: Phosphorylation destabilizes alpha-helices.
abstract: Phosphorylation of threonine destabilizes the leucine zipper of a bZIP protein by 4.6 kcal mol-1 dimer-1, which reduces DNA binding 100-fold. This decrease in stability reflects the low alpha-helix forming propensity of a phosphorylated threonine.
authors.forename: L
authors.initials: L
authors.lastname: Szilák
authors.forename: J
authors.initials: J
authors.lastname: Moitra
authors.forename: D
authors.initials: D
authors.lastname: Krylov
authors.forename: C
authors.initials: C
authors.lastname: Vinson
mesh_terms: D000595:Amino Acid Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D001665:Binding Sites; D002151:Calorimetry; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D004355:Drug Stability; D004926:Escherichia coli; D051790:G-Box Binding Factors; D016350:Leucine Zippers; D008961:Models, Structural; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010769:Phosphothreonine; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013912:Threonine; D014157:Transcription Factors
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D011994:Recombinant Proteins; D014157:Transcription Factors; D010769:Phosphothreonine; D013912:Threonine