[The effect of alimentary vitamin B6 supply during pregnancy and lactation on the activity of specific transaminases of lactating rats].
Eighty female Sprague-Dawley rats weighing 257 g were fed during gravidity a semi-synthetic diet containing five vitamin-B6-treatment groups (0.6, 3, 6, 18 and 180 mg/kg diet). The daily food intake was 14 g. During the following lactation the rats of each treatment group were divided into two groups containing 3 and 6 mg vitamin B6. At the 14th day of lactation the dams were decapitated. Parameters for determination of the vitamin-B6-status were activity of AST and ALT in plasma, erythrocytes and liver. The average activity of AST in plasma was 549 U/l, in erythrocytes 1939 U/l and liver 106 U/g fresh matter (FM). The increasing vitamin-B6-supplementation during gravidity resulted in an elevated activity of AST between lowest and highest treatment group in plasma 56%, erythrocytes 44%, and in liver 43%, respectively. In response to the increasing vitamin-B6-treatment during lactation the activity of AST in plasma increased for 19%, in erythrocytes for 13%, and in liver for 24%, respectively. A low vitamin-B6-supply (0.6 mg/kg diet) during gravidity in combination with demand-oriented supply during lactation (6 mg/kg diet) initiated the highest increase of activity. A deficient vitamin-B6-supply during lactation (3 mg/kg diet) could be compensated with optimal vitamin-B6-supply during gravidity. The values of ALT-activity showed no significant differences between the graded vitamin-B6-supplements, as a result of a high coenzyme saturation.
Roth-Maier
D A
DA
Institut für Ernährungsphysiologie, Technische Universität München, Freising-Weihenstephan.
Benedikt
J
J
Kirchgessner
M
M
D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D007774:Lactation / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011736:Pyridoxine / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D011736:Pyridoxine