PCR site-directed mutagenesis using Pyrococcus sp GB-D polymerase coupled to a rapid screening procedure. Application to a beta-glucanase gene.
Pons
J
J
Instituto de Biologia Fundemental, University Autónoma de Barcelona, Spain.
Planas
A
A
Juncosa
M
M
Querol
E
E
D001105:Archaea / Q000201:enzymology; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D004274:DNA, Recombinant / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D007202:Indicators and Reagents; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013698:Templates, Genetic
D017931:DNA Primers; D004274:DNA, Recombinant; D007202:Indicators and Reagents; D011994:Recombinant Proteins; C105117:Pyrococcus sp GB-D DNA polymerase; D004259:DNA-Directed DNA Polymerase; D006026:Glycoside Hydrolases; C028020:licheninase