title: Synthesis and luminescence spectral characterization of long-lifetime lipid metal-ligand probes.
abstract: We synthesized phospholipid analogues of phosphatidyl ethanolamine which contains a ruthenium metal-ligand complex (MLC) covalently bound to the amino group. Two analogues were synthesized, containing either one (Ru-PE) or two (Ru-PE2) lipid molecules covalently linked to the MLC by the amino group of the lipid. These MLC-lipid probes display intensity decay times from 682 to 357 ns, depending on temperature. Importantly, the luminescence MLC groups display polarized emission, enabling their use for studies of membrane dynamics. The long intensity decay times allowed measurement of the overall rotation correlation time of lipid vesicles to several microseconds. The spectral properties of the model membranes containing Ru-PE or Ru-PE2 were independent of the probe-to-lipid molar ratio from 1:20 to 1:100, suggesting minimal tendency for probe-probe interactions. These MLC-lipid probes can be expected to have numerous applications in studies of membrane dynamics on the microsecond timescale.
authors.affiliation: Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore 21201, USA.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Szmacinski
authors.forename: J R
authors.initials: JR
authors.lastname: Lakowicz
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008024:Ligands; D009942:Organometallic Compounds / Q000138:chemical synthesis; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D009942:Organometallic Compounds; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D015082:2,2'-Dipyridyl; C058014:ruthenium bis(bipyridine)dicarboxybipyridine; D012428:Ruthenium; C030345:1,2-dipalmitoylphosphatidylglycerol