Characterization of two new matrices for matrix-assisted laser desorption/ionization mass spectrometry.
Three structurally related compounds, 4-hydroxy-33-methoxyphenylpyruvic acid (HMPPA), indole-3 pyruvic acid (IPA), and indole-3-glyoxylic acid have been evaluated as matrix-assisted laser desorption/ionization (MALDI) matrices. HMPPA and IPA were found to be effective matrices for MALDI-MS analysis of proteins and peptides and have been characterized in this work. HMPPA was found to be a particularly good matrix for the analysis of proteins and peptides because of its tolerance towards impurities and the resulting sensitivity in MALDI-MS experiments. Analysis of model proteins and peptides, including those in biological fluid, are demonstrated.
Bai
J
J
Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.
Liang
X
X
Liu
Y H
YH
Zhu
Y
Y
Lubman
D M
DM
D006038:Glyoxylates / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010667:Phenylpyruvic Acids / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006038:Glyoxylates; D007211:Indoles; D010455:Peptides; D010667:Phenylpyruvic Acids; D011506:Proteins; C016827:vanilpyruvic acid; C103608:indole-3-glyoxylic acid; C008122:indol-3-yl pyruvic acid