title: [Isolation and physico-chemical characteristics of Staphylococcus hyaluronidase].
abstract: A scheme for purification of hyaluronidase from Staphylococcus aureus 0-15 that allows to produce a 550-fold-purified preparation with a high specific activity was developed. Hyaluronidase was found to consist of two molecular forms with different molecular weights. Staphylococcus hyaluronidase referred to as a complex preparation and its molecular forms have action optimum at pH 5.0-7.2 and at a temperature between 30 and 37 degrees C. The enzymes were highly specific to their substrate: they catalyzed the depolimerization of hyaluronic acid but did not split the other glycosaminoglycans.
authors.forename: T S
authors.initials: TS
authors.lastname: Turmanidze
authors.forename: G I
authors.initials: GI
authors.lastname: Kvesitadze
authors.forename: I U
authors.initials: IU
authors.lastname: Mikanadze
authors.forename: M G
authors.initials: MG
authors.lastname: Shanidze
authors.forename: M Z
authors.initials: MZ
authors.lastname: Loladze
authors.forename: T V
authors.initials: TV
authors.lastname: Birkadze
authors.forename: M G
authors.initials: MG
authors.lastname: Alibegashvili
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000302:isolation & purification*; D013210:Staphylococcus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D006821:Hyaluronoglucosaminidase