title: Isolation of a novel chick homolog of Serrate and its coexpression with C-Notch-1 in chick development.
abstract: Intercellular signaling mediated by the transmembrane proteins, Notch as receptor and its ligands, Delta and Serrate, plays essential roles in the developmental fate decision of many cell types in Drosophila. The Notch genes are highly conserved both in invertebrates and in vertebrates, suggesting that Notch pathway regulates cell fate decisions during vertebrates development. Notch, Delta and Serrate homologs in chicken have been cloned (Henrique et al., Nature 375: 787-790, 1995; Myat et al., Dev. Biol. 174: 233-247, 1996). We isolated a novel chick homolog of Drosophila Serrate, named C-Serrate-2, and examined its expression patterns during the early chick development using whole-mount in situ hybridization. C-Serrate-2 transcripts were detected in several tissues including the forebrain, the myotome and the apical ectodermal ridge (AER) of the limb bud of a 4-day-old chick embryo. In most of the regions where C-Serrate-2 was expressed, C-Notch-1 was also expressed. Our observations suggest that Serrate-2-Notch-1 signaling plays a role in a variety of morphogeneses during the chick development.
authors.affiliation: Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Mochii
authors.forename: R
authors.initials: R
authors.lastname: Kodama
authors.forename: Y
authors.initials: Y
authors.lastname: Hamada
authors.forename: N
authors.initials: N
authors.lastname: Mizuno
authors.forename: G
authors.initials: G
authors.lastname: Eguchi
authors.forename: C
authors.initials: C
authors.lastname: Tachi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins; D002642:Chick Embryo; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D005121:Extremities / Q000196:embryology; D015870:Gene Expression; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000235:genetics; D051880:Receptors, Notch; D017385:Sequence Homology; D000072099:Serrate-Jagged Proteins; D013687:Telencephalon / Q000737:chemistry / Q000196:embryology / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D029721:Drosophila Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; C497759:N protein, Drosophila; D011956:Receptors, Cell Surface; D051880:Receptors, Notch; C000606278:Ser protein, Drosophila; D000072099:Serrate-Jagged Proteins