Expression of the nucleocapsid protein gene of the canine distemper virus.
We constructed a cDNA clone of canine distemper virus (CDV) encoding an entire nucleoprotein (NP) gene, by means of the reverse transcription-polymerase chain reaction (RT-PCR). The cloned NP gene was inserted into the eucaryotic expression vector, pRVSV. After transfection of the plasmid into Vero cells, we examined the expression of CDV-specific NP antigen by means of indirect immunofluorescence assay (IFA) and Western blotting, using various antibodies against NP of CDV and an antiserum against NP of measles virus. The CDV-NP specific antigen was detected in the nuclei of the cells transfected with pRV-ON, by means of IFA with antibodies specific to the NP.
Shin
Y S
YS
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Mori
T
T
Tomonaga
K
K
Iwatsuki
K
K
Kai
C
C
Mikami
T
T
D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004217:Distemper Virus, Canine / Q000235:genetics* / Q000378:metabolism*; D004285:Dogs; D008969:Molecular Sequence Data; D019251:Nucleocapsid / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D014709:Vero Cells
D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins