An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia).
Sequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.
Fernandes
O
O
Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
Bozza
M
M
Pascale
J M
JM
de Miranda
A B
AB
Lopes
U G
UG
Degrave
W M
WM
D000818:Animals; D001483:Base Sequence; D018105:DNA, Kinetoplast / Q000032:analysis* / Q000302:isolation & purification; D006824:Hybridization, Genetic; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification*; D007892:Leishmania braziliensis / Q000235:genetics / Q000302:isolation & purification; D018334:Leishmania guyanensis / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018105:DNA, Kinetoplast; D009841:Oligonucleotides