Definition of a large region of RAG1 that is important for coimmunoprecipitation of RAG2.
Interaction between the RAG1 and RAG2 proteins is probably critical for V(D)J recombination. Using a coimmunoprecipitation assay, we define a large region of RAG1 (amino acids 504-1008) that is sufficient for interaction with RAG2. This region comprises the C-terminal half of the RAG1 protein, and is within the region defined as the recombinationally active core. Deletion of either of two regions of RAG1 (amino acids 504-570 or 850-1008) causes a loss of interaction with RAG2. Loss of coimmunoprecipitation is also seen with RAG1 core proteins containing deletions of smaller stretches of amino acids (amino acids 506-511 or 545-550), emphasizing the importance of this region of RAG1 in forming a complex with RAG2. A variety of other small deletion mutations within the amino acid region 504-1008 also decrease coimmunoprecipitation of RAG2 with RAG1, indicating that much or all of this region is important for complex formation.
McMahan
C J
CJ
Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
Sadofsky
M J
MJ
Schatz
D G
DG
D000818:Animals; D002467:Cell Nucleus / Q000235:genetics / Q000276:immunology; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051379:Mice; D016296:Mutagenesis / Q000276:immunology; D011233:Precipitin Tests; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic / Q000276:immunology*; D017384:Sequence Deletion / Q000276:immunology; D014162:Transfection / Q000276:immunology
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D011506:Proteins; C499308:Rag2 protein, mouse; D011948:Receptors, Antigen, T-Cell; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein