title: Dinuclear lanthanum(III) complex for efficient hydrolysis of RNA.
abstract: A dinuclear La3+ complex hydrolyzes the phosphodiester bond in diribonucleotides efficiently under mild conditions. The rate of hydrolysis is remarkably accelerated on the complex formation between La3+ and TPHP (TPHP = N,N,N',N'-tetrakis [(2-pyridyl)methyl]-2-hydroxy-1,3-diaminopropane), and the highest activity is attained when the dinuclear structure is formed. The half-life of ApA hydrolysis by the dinuclear La3+ complex (1 mM) is 350 s, whereas that by the free La3+ ion (1 mM) is 23,000 s at pH 7.2 and 50 degrees C. The dinuclear La3+ complex is promising as a catalytic center of artificial nucleases.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo.
authors.forename: M
authors.initials: M
authors.lastname: Yashiro
authors.forename: A
authors.initials: A
authors.lastname: Ishikubo
authors.forename: M
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D001665:Binding Sites; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D007811:Lanthanum / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011725:Pyridines / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism*
chemical_list: D007202:Indicators and Reagents; C102154:N,N,N',N'-tetrakis((2-pyridyl)methyl)-2-hydroxy-1,3-diaminopropane; D011725:Pyridines; C028521:lanthanum chloride; D012313:RNA; D007811:Lanthanum