Primary culture of chicken hepatocytes in serum-free medium (pH 7.8) secreted albumin and transferrin for a long period in free gas exchange with atmosphere.
To study liver functions of chicken, we examined the primary culture of chicken hepatocytes, and found an easy method of long-term culture with free atmosphere exchange. Chicken hepatocytes were obtained by collagenase perfusion and cultured at 37 degrees C as a monolayer without substratum in serum-free L-15 medium (pH 7.8) with free atmosphere exchange. The amounts of albumin and transferrin in medium were assayed by ELISA. The culture of chicken hepatocytes was maintained in the serum-free L15-medium )pH 7.) and 37 degrees C with free atmosphere exchange for 20 days. The amount of albumin secreted in the medium decreased to low levels early in culture; however, this was followed by marked increase from day 9 to day 17 of culture. The amount of transferrin was constant until day 6, then it too increased with further culture. We reported an easy method for the simple monolayer culture of chicken hepatocytes in serum-free L12 medium (pH 7.8) with free atmosphere exchange over an extended period. Expression of liver-specific functions, viz. albumin and transferrin synthesis, was observed after 1 week of culture.
Fujii
M
M
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Japan.
Yoshino
I
I
Suzuki
M
M
Higuchi
T
T
Mukai
S
S
Aoki
T
T
Fukunaga
T
T
Sugimoto
Y
Y
Inoue
Y
Y
Kusuda
J
J
Saheki
T
T
Sato
M
M
Hayashi
S
S
Tamaki
M
M
Sugano
T
T
D000388:Air; D000818:Animals; D000918:Antibody Specificity; D002478:Cells, Cultured; D002645:Chickens; D016895:Culture Media, Serum-Free; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D012709:Serum Albumin / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014168:Transferrin / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*
D016895:Culture Media, Serum-Free; D012709:Serum Albumin; D014168:Transferrin