Detection of the single-stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone-like protein.
Streptomyces plasmid pSA1.1 accumulated single-stranded DNA as replication intermediates in S. lividans; therefore, this plasmid was considered to replicate by a rolling-circle mechanism. A DNA-binding protein (pI > 9.7 and about 10 kDa) was purified on a denatured DNA-Cellulose column, then on a native DNA-Cellulose column. The N-terminal amino acid sequence of this protein has a high homology with bacterial histone-like proteins. In the gel retardation assay, this protein bound with the single-stranded DNA of pSA1.1. We propose that this protein may participate in the replication of pSA1.1.
Yokoyama
E
E
Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Doi
K
K
Kimura
M
M
Ogata
S
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000302:isolation & purification* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D006657:Histones