Rapid molecular haplotyping of the first exon of the human dopamine D4 receptor gene by heteroduplex analysis.
The dopamine D4 receptor gene (DRD4) and its products are of great interest in many neurospsychiatric disorders. There are at least five expressed polymorphisms in exons 1 and 3, plus rare expressed variants, all of which may have functional relevance. Several studies have described methods for studying the exon 3 polymorphisms, especially the VNTR; fewer reports have documented the exon 1 polymorphisms and variants of DRD4. We report here a simple, rapid, nonisotopic, nondenaturing heteroduplex method for determining the molecular haplotype composed of the two more polymorphic systems of the first exon of DRD4: the 12 bp duplication and 13 bp deletion. This method will facilitate future research on expressed variation of this gene.
Chang
F M
FM
Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Kidd
K K
KK
D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons / Q000235:genetics; D005838:Genotype; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D050638:Receptors, Dopamine D4; D017384:Sequence Deletion / Q000235:genetics; D014407:Tumor Cells, Cultured
C493599:DRD4 protein, human; D009692:Nucleic Acid Heteroduplexes; D017448:Receptors, Dopamine D2; D050638:Receptors, Dopamine D4