Prenylation of oncogenic human PTP(CAAX) protein tyrosine phosphatases.
Many isoprenylated proteins are known to participate in signal transduction, but not all have been identified. Using an in vitro prenylation screen, two human cDNAs (PTP(CAAXI) and PTP(CAAX2)) homologous to the rat PRL-1 and human OV-1 protein tyrosine phosphatase genes were identified. PTP(CAAXI) and PTP(CAAX2) were farnesylated in vitro by mammalian farnesyl:protein transferase, and epitope-tagged PTP(CAAX2) was prenylated in epithelial cells. Overexpression of PTP(CAAXI) and PTP(CAAX2) in epithelial cells caused a transformed phenotype in culture and tumor growth in nude mice. Thus, PTP(CAAXI) and PTP(CAAX2) represent a novel class of isoprenylated, oncogenic protein tyrosine phosphatases.
Cates
C A
CA
Department of Biology, Indiana University-Purdue University at Indianapolis, 46202, USA.
Michael
R L
RL
Stayrook
K R
KR
Harvey
K A
KA
Burke
Y D
YD
Randall
S K
SK
Crowell
P L
PL
Crowell
D N
DN
D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D010641:Phenotype; D017368:Protein Prenylation; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D018797:Cell Cycle Proteins; D017874:Immediate-Early Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C083291:geranylgeranyltransferase type-I; C064911:p21(ras) farnesyl-protein transferase; C490198:PTP4A1 protein, human; C487561:PTP4A2 protein, human; D017027:Protein Tyrosine Phosphatases; C087359:Ptp4a1 protein, mouse; C490199:Ptp4a1 protein, rat