Toxoplasma antibody titers by ELISA and indirect latex agglutination test in pregnant women.
The seroepidemiologic studies on anti-Toxoplasma antibody titers were carried out using ELISA and indirect latex agglutination test. Among 899 sera prepared from pregnant women, 39 cases (4.3%) revealed positive reaction and 218 sera from middle school students showed 4 positive reaction (1.8%) by ELISA. By LAT (newly established by National Veterinary Research Institute. Korea), the sera of 7 pregnant women (0.8%) showed positive reaction. When 80 sera showing > or = 1:8 by LAT were used for comparing the results obtained from LAT and Toxotest-MT (Eiken Chemical Co., Japan), 7 cases and 8 sera were positive, respectively. All of 11 sera of proven toxoplasmosis patients showed positive reaction in both tests. Overall proportion of agreement between LAT kit and Toxotest-MT was 0.94 (kappa-index = 0.632, p < 0.011, and LAT was considered to be useful for the screening of toxoplasmosis.
Ryu
J S
JS
Department of Parasitology, College of Medicine, Hanyang University, Seoul, Korea.
Min
D Y
DY
Ahn
M H
MH
Choi
H G
HG
Rho
S C
SC
Shin
Y J
YJ
Choi
B
B
Joo
H D
HD
D000293:Adolescent; D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007841:Latex Fixation Tests / Q000379:methods*; D008297:Male; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis / Q000453:epidemiology*; D015995:Prevalence; D011933:Reagent Kits, Diagnostic; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis / Q000453:epidemiology*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic