A low content of ERCC1 and a 120 kDa protein is a frequent feature of group F xeroderma pigmentosum fibroblast cells.
The ERCC1 protein has been predicted to form part of a tight complex with a protein partner, the yet-unidentified XPF/ERCC4 protein, in normal human cells. We used an anti-ERCC1 antibody to detect the complex by immunoprecipitation and immunoblotting. The amount of ERCC1 protein expressed in five different XP-F cell strains was 1/ 5-1/34 of that of the protein in normal and XP cell strains representing other complementation groups. A 120 kDa protein was co-immunoprecipitated with ERCC1 by the anti-ERCC1 antibody, and the amount of the 120 kDa protein in XP-F cell strains was 1/5-1/8 of that of the protein in normal and XP cell strains representing other complementation groups. The XPA protein was not co-immunoprecipitated with ERCC1 in any cell strain. These results demonstrate that a low level of ERCC1 and the 120 kDa protein is a frequent feature of XP-F cell extracts and that a lower amount of a complex between these proteins occurs in XP-F cells than in normal cells.
Yagi
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T
Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Japan.
Wood
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RD
Takebe
H
H
D000906:Antibodies; D002457:Cell Extracts / Q000276:immunology; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D011233:Precipitin Tests; D011506:Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D014983:Xeroderma Pigmentosum / Q000378:metabolism* / Q000473:pathology
D000906:Antibodies; D002457:Cell Extracts; D004268:DNA-Binding Proteins; D011506:Proteins; C071447:ERCC1 protein, human; D004720:Endonucleases