Cloning gene family members using PCR with degenerate oligonucleotide primers.
Preston
G M
GM
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
D000595:Amino Acid Sequence; D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins; D001789:Blood Group Antigens; D003001:Cloning, Molecular / Q000379:methods*; D016384:Consensus Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D005333:Fetus; D015723:Gene Library; D006801:Humans; D007202:Indicators and Reagents; D007473:Ion Channels / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D009711:Nucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D014981:Xenopus
C496020:AQP1 protein, human; D020346:Aquaporins; D001789:Blood Group Antigens; D017931:DNA Primers; D018076:DNA, Complementary; D007202:Indicators and Reagents; D007473:Ion Channels; D009711:Nucleotides; D051398:Aquaporin 1