title: Cloning and sequence of a gene for a homologue of the C subunit of the V-ATPase from the salivary gland of the tick Amblyomma americanum (L).
abstract: A 1084 base pair partial cDNA showing similarity to the C subunit of the vacuolar ATPase (V-ATPase) was isolated on a clone from a cDNA library made from salivary glands from 3-day-old feeding adult Amblyomma americanum (L.) female ticks. The 5' end was completed using primer extension and the two pieces joined to form a complete cDNA of 1373 bp. This mRNA is expressed in embryos and the salivary glands of unfed adults and adult females at all stages of feeding. Specific inhibitors of the V-ATPase decrease the rate of dopamine-stimulated secretion of isolated salivary glands, but not as much as ouabain, an inhibitor of the Na+, K+ ATPase, indicating that a V-ATPase may participate in the mechanism of salivary fluid secretion in A. americanum, but the volume of saliva secreted is more dependent on an active Na+, K+ ATPase.
authors.affiliation: Department of Entomology, Oklahoma State University, Stillwater 74078, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: McSwain
authors.forename: C
authors.initials: C
authors.lastname: Luo
authors.forename: G A
authors.initials: GA
authors.lastname: deSilva
authors.forename: M J
authors.initials: MJ
authors.lastname: Palmer
authors.forename: J S
authors.initials: JS
authors.lastname: Tucker
authors.forename: J R
authors.initials: JR
authors.lastname: Sauer
authors.forename: R C
authors.initials: RC
authors.lastname: Essenberg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D018942:Macrolides; D008969:Molecular Sequence Data; D010042:Ouabain / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000235:genetics*; D012469:Salivary Glands / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013987:Ticks / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D025262:Vacuolar Proton-Translocating ATPases
chemical_list: D000900:Anti-Bacterial Agents; D018076:DNA, Complementary; D018942:Macrolides; C057620:bafilomycin A; D010042:Ouabain; C036978:concanamycin A; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D005033:Ethylmaleimide