Reactions of isocytochrome c2 in the photosynthetic electron transfer chain of Rhodobacter sphaeroides.
Rhodobacter sphaeroides strains lacking cytochrome c2 (cyt c2), the normal electron donor to P870+ in light-oxidized reaction center (RC) complexes, are unable to grow photosynthetically. However, spd mutations that suppress the photosynthetic deficiency of cyt c2 mutants elevate levels of the cyt c2 isoform, isocyt c2. We monitored photosynthetic electron transfer in whole cells, in chromatophores, and with purified components to ascertain if and how isocyt c2 reduced light-oxidized RC complexes. These studies revealed that several fundamental aspects of photosynthetic electron transfer were similar in strains that use isocyt c2 and wild-type cells. For example, P870+ reduction accompanied cytochrome c oxidation. In addition, photosynthetic electron transfer was blocked by the well-known cyt bc1 complex inhibitors antimycin and myxothiazol. However, even at the increased isocyt c2 levels present in these strains (approximately 40% that of cyt c2 in wild-type cells), there was little, if any, of the rapid (< 5 microns) electron transfer to P870+ that is characteristic of cytochromes bound to RC complexes at the time of the light flash. Thus, it appears that isocyt c2 function limits the in vivo rate of P870+ reduction. Indeed, at low ionic strength in vitro, the apparent affinity of isocyt c2 for RC complexes (KD approximately 40 microM) is significantly lower than that of cyt c2 (KD approximately 1.0 microM). This reduced affinity does not appear to result from an altered mode of RC binding by isocyt c2 since electrostatic interactions make similar overall contributions to the binding of both cyt c2 and isocyt c2 to this membrane-bound redox partner. Thus, sequence, structural, or local conformational differences between cyt c2 and isocyt c2 significantly alter their apparent affinities for this physiologically relevant redox partner.
Witthuhn
V C
VC
Bacteriology Department, University of Wisconsin-Madison 53706, USA.
Gao
J
J
Hong
S
S
Halls
S
S
Rott
M A
MA
Wraight
C A
CA
Crofts
A R
AR
Donohue
T J
TJ
D003574:Cytochrome c Group / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D045304:Cytochromes c; D045362:Cytochromes c2; D004579:Electron Transport / Q000528:radiation effects; D007700:Kinetics; D009154:Mutation; D010084:Oxidation-Reduction; D010777:Photochemistry; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000528:radiation effects; D012242:Rhodobacter sphaeroides / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D055672:Static Electricity
D003574:Cytochrome c Group; D045322:Photosynthetic Reaction Center Complex Proteins; C032139:iso-2-cytochrome C; D045304:Cytochromes c; D045362:Cytochromes c2