Cloning and characterization of Chinese hamster p53 cDNA.
We have cloned and sequenced Chinese hamster p53 cDNA and have compared the p53 sequence in different Chinese hamster cell lines to several relevant phenotypes. Our results indicate that a mutation in CHO cells that changes Thr211 to Lys211 abrogates the ability to arrest in G1 and apparently renders cells capable of amplifying DNA. However, this mutation has no effect on the G2 checkpoint or on acute down-regulation of DNA replication after a radiation challenge.
Lee
H
H
Department of Radiation Oncology, University of Virginia School of Medicine, Charlottesville 22908, USA.
Larner
J M
JM
Hamlin
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D018076:DNA, Complementary; D016193:G1 Phase / Q000235:genetics; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016196:S Phase / Q000235:genetics
D018076:DNA, Complementary