Characterisation of atypical biotype 3, serotype O:3 Yersinia and development of a simple identification scheme.
Five clinical strains of Yersinia isolated in Japan and identified as atypical biotype 3 or biotype 3B, serotype O:3, phage type II (3*/O:3/II) Yersinia enterocolitica were characterised since the biochemical reactions of these strains indicate they might also belong to the species Yersinia bercovieri. Biochemical tests, characterisation of the beta-lactamases and DNA-DNA hybridization studies provided strong evidence indicating that these strains should be classified as Yersinia enterocolitica. A simple scheme combining a disc diffusion test and four biochemical tests was devised for identification of these atypical strains.
Pham
J N
JN
Department of Microbiology, Prince of Wales Hospital, Randwick, New South Wales, Australia.
Bell
S M
SM
Guilvout
I
I
Martin
L
L
Carniel
E
E
D000082:Acetaminophen / Q000378:metabolism; D000581:Amidohydrolases / Q000378:metabolism; D000983:Antipyrine / Q000031:analogs & derivatives / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002228:Carbenicillin / Q000494:pharmacology; D002697:Chloral Hydrate / Q000031:analogs & derivatives / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D004150:Dipeptidases / Q000378:metabolism; D004338:Drug Combinations; D005643:Fucose / Q000378:metabolism; D006801:Humans; D007525:Isoelectric Focusing; D008744:Methylamines / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009693:Nucleic Acid Hybridization; D010406:Penicillins / Q000494:pharmacology; D012703:Serotyping; D013982:Ticarcillin / Q000494:pharmacology; D015007:Yersinia / Q000145:classification* / Q000378:metabolism / Q000472:pathogenicity; D015009:Yersinia Infections / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000145:classification* / Q000378:metabolism / Q000472:pathogenicity; D001618:beta-Lactamases / Q000032:analysis / Q000096:biosynthesis
D004269:DNA, Bacterial; D004338:Drug Combinations; D008744:Methylamines; D010406:Penicillins; D005643:Fucose; D000082:Acetaminophen; D002697:Chloral Hydrate; C008527:Midrin; D004150:Dipeptidases; C022449:proline dipeptidase; D000581:Amidohydrolases; C022490:pyrazinamide deamidase; D001618:beta-Lactamases; D013982:Ticarcillin; D002228:Carbenicillin; D000983:Antipyrine