Multicomponent investigations of the hydrogen peroxide- and hydroxyl radical-scavenging antioxidant capacities of biofluids: the roles of endogenous pyruvate and lactate. Relevance to inflammatory joint diseases.
High field proton (1H) nuclear magnetic resonance (NMR) analysis of biofluids (health human blood sera and inflammatory knee-joint synovial fluids) has been employed to evaluate the hydrogen peroxide (H2O2)- and hydroxyl radical (0OH)- scavenging antioxidant capacities of a range of polar, low-molecular-mass endogenous metabolites therein. Data obtained indicate that consumption of H2O2 by pyruvate (generating acetate and CO2 via an oxidative decarboxylation reaction) and 0OH radical by lactate (generating pyruvate, and subsequently acetate and CO2) may serve to protect alternative biofluid components (e.g., macromolecules) against reactive oxygen species-mediated oxidative damage in vivo. The mechanistic, physiological and potential therapeutic implications of these results are discussed with special reference to inflammatory joint diseases.
Herz
H
H
Inflammation Research Group, London Hospital Medical College, UK.
Blake
D R
DR
Grootveld
M
M
D000328:Adult; D000368:Aged; D001168:Arthritis / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000737:chemistry / Q000378:metabolism*; D007719:Knee Joint; D019344:Lactic Acid / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D019289:Pyruvic Acid / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013582:Synovial Fluid / Q000378:metabolism*
D016166:Free Radical Scavengers; D017665:Hydroxyl Radical; D019344:Lactic Acid; D019289:Pyruvic Acid; D006861:Hydrogen Peroxide