Crystallization and preliminary x-ray analysis of recombinant staphylokinase.
Diffraction quality crystals of recombinant staphylokinase (STAR) have been grown by the hanging drop vapor diffusion technique from a solution containing MgCl2, Tris buffer (pH 8.5), and polyethylene glycol 4000. The crystals belong to the monoclinic space group C2 with unit cell dimensions alpha = 60.6 A, b = 43.7 A, c = 54.3 A, and beta = 115.6 degrees. A complete native data set to 1.8 A resolution has been collected using synchrotron radiation.
Rabijns
A
A
Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
Baeyens
K
K
De Bondt
H L
HL
De Ranter
C
C
D003460:Crystallization; D008666:Metalloendopeptidases / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus