title: Non-muscarinic- and non-adrenergic-mediated effects of lindane on phosphoinositide hydrolysis in rat brain cortex slices.
abstract: The influence of lindane upon phosphatidylinositol hydrolysis in rat brain cortex slices has been investigated using anion-exchange chromatography in order to separate the water-soluble inositol metabolites. Acetylcholine, noradrenaline, and lindane induce the accumulation of myo-[2-3H]inositol as the water-soluble inositol metabolites. However, the cholinergic muscarinic antagonist atropine inhibited the stimulatory response of carbachol, but practically unmodified the effect that lindane has on inositol phosphate production. Also, prazosin anti-alpha 1 adrenoreceptors blocked noradrenaline-induced phosphoinositide hydrolysis, but had no effect on lindane-induced increase of inositol phosphate levels. The results suggest that lindane does not exert a general effect on the receptor-stimulated formation of inositol phosphates by both muscarinic and alpha 1-adrenergic agonists.
authors.affiliation: Departamento de Bioquimica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Pajuelo
authors.forename: J A
authors.initials: JA
authors.lastname: Sánchez-Alonso
authors.forename: N
authors.initials: N
authors.lastname: del Hoyo
authors.forename: J A
authors.initials: JA
authors.lastname: Pulido
authors.forename: M A
authors.initials: MA
authors.lastname: Pérez-Albarsanz
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D006868:Hydrolysis; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011941:Receptors, Adrenergic / Q000187:drug effects*; D011976:Receptors, Muscarinic / Q000187:drug effects*
chemical_list: D007306:Insecticides; D010716:Phosphatidylinositols; D011941:Receptors, Adrenergic; D011976:Receptors, Muscarinic; D001556:Hexachlorocyclohexane; D001285:Atropine; D002217:Carbachol; D009638:Norepinephrine; D011224:Prazosin