Structural studies of the exopolysaccharide produced by Lactobacillus paracasei 34-1.
The exopolysaccharide produced by Lactobacillus paracasei 34-1 in a semi-defined medium was found to be a heteropolymer, composed of D-galactose, 2-acetamido-2-deoxy-D-galactose, and sn-glycerol 3-phosphate in molar ratios of 3:1:1. By means of deglycerophosphorylation, methylation analysis, and 1D/2D NMR studies (1H, 13C, and 31P) the polysaccharide was shown to consist of repeating units with the following structure: [formula: see text].
Robijn
G W
GW
Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Wienk
H L
HL
van den Berg
D J
DJ
Haas
H
H
Kamerling
J P
JP
Vliegenthart
J F
JF
D000116:Acetylgalactosamine / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005690:Galactose / Q000032:analysis; D005994:Glycerophosphates / Q000032:analysis; D007778:Lactobacillus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification
D005994:Glycerophosphates; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; C029620:alpha-glycerophosphoric acid; D000116:Acetylgalactosamine; D005690:Galactose