Down-regulation of striatal enkephalinergic (PPA) messenger RNA without prior apoptotic features following reversible focal ischemia in rat.
In order to determine whether striatal enkephalinergic neurons were affected by reversible focal ischemia, we have investigated the expression of the preproenkephalin (PPA) messenger by in situ hybridization (ISH) combined with TUNEL staining to display apoptosis in the same rat brain sections. Our data demonstrated a massive reduction of the number of PPA-mRNA containing neurons concomitant with the emergence of apoptotic cells. However, double-labeled neurons (ISH- and TUNEL-positive cells) were not detected, suggesting that either disruption of mRNA precedes DNA fragmentation or ischemia leads to a long lasting reduction of mRNA(s) without damage.
Popovici
T
T
Université René Descartes-INSERM U29, Paris, France. popovici@u29.cochin.inserm.fr
Margaill
I
I
Plotkine
M
M
Ben-Ari
Y
Y
Charriaut-Marlangue
C
C
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001157:Arterial Occlusive Diseases / Q000503:physiopathology; D001710:Biotin; D002545:Brain Ischemia / Q000503:physiopathology*; D053938:DNA Fragmentation; D003856:Deoxyuracil Nucleotides; D004745:Enkephalins / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D017072:Neostriatum / Q000098:blood supply / Q000502:physiology*; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
D003856:Deoxyuracil Nucleotides; D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D001710:Biotin; C033660:preproenkephalin