[Effect of dibucaine and lidocaine on histamine release from mouse bone marrow-derived cultured mast cells].
We examined the effects of dibucaine and lidocaine on histamine release from mouse bone marrow-derived cultured mast cells. The effects of these drugs on intracellular calcium were also monitored by assessing Fura-2 signals. Additionally, the inhibitory effects of lidocaine on IgE dependent and independent stimuli were examined. Though dibucaine induced histamine release and increases in intracellular calcium from mast cells dose-dependently, lidocaine did not. Lidocaine inhibited both the IgE-dependent and independent histamine release from mast cells in a dose dependent manner. However, the ability of lidocaine to inhibit the IgE-dependent response was greater. Lidocaine also inhibited increases in intracellular calcium to a greater extent after IgE-dependent stimulation as compared with IgE-independent stimulation. The degree of the inhibition of histamine release by lidocaine appeared to parallel decreases in calcium mobilization.
Yanagi
H
H
Department of Anesthesiology, Kyorin University School of Medicine, Mitaka.
Ozawa
R
R
Kobayashi
M
M
Sankawa
H
H
Saito
H
H
D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003864:Depression, Chemical; D003992:Dibucaine / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E / Q000378:metabolism; D008012:Lidocaine / Q000494:pharmacology*; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice
D000779:Anesthetics, Local; D007073:Immunoglobulin E; D008012:Lidocaine; D003992:Dibucaine; D002118:Calcium