title: Combinatorial signalling by Xwnt-11 and Xnr3 in the organizer epithelium.
abstract: The epithelium of the Spemann organizer plays an important role in embryonic axis formation and transplantation experiments have shown that epithelial organizer cells have potent axis-inducing potential. Known axis-inducing molecules like noggin and chordin are not expressed in the epithelium and cannot account for its inductive properties. Xwnt-11 is expressed in the epithelium but has only poor dorsalizing activity. In an expression screen for genes that are able to functionally cooperate with Xwnt-11 we have identified a cDNA encoding Xenopus nodal-related 3 (XNR3), a member of the TGF-beta family, coexpressed with Xwnt-11 in the organizer epithelium. Xwnt-11 and Xnr3 act highly cooperatively in inducing secondary embryonic axes and dorsalizing ventral mesoderm. Xwnt-11/Xnr3 interfere with BMP signalling without themselves inducing chordin or noggin. The results indicate that induction by the organizer epithelium may result from the combinatorial action of instructive Xnr3 and permissive Xwnt-11 signalling.
authors.affiliation: Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Glinka
authors.forename: H
authors.initials: H
authors.lastname: Delius
authors.forename: C
authors.initials: C
authors.lastname: Blumenstock
authors.forename: C
authors.initials: C
authors.lastname: Niehrs
mesh_terms: D000818:Animals; D015415:Biomarkers; D019521:Body Patterning; D003001:Cloning, Molecular; D004627:Embryonic Induction; D004848:Epithelium / Q000637:transplantation; D006023:Glycoproteins / Q000494:pharmacology / Q000502:physiology*; D017403:In Situ Hybridization; D008648:Mesoderm / Q000187:drug effects; D012333:RNA, Messenger / Q000494:pharmacology; D018125:Receptors, Transforming Growth Factor beta / Q000502:physiology*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000235:genetics; D051153:Wnt Proteins; D014981:Xenopus; D029867:Xenopus Proteins
chemical_list: D015415:Biomarkers; D006023:Glycoproteins; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D051153:Wnt Proteins; D029867:Xenopus Proteins; C094620:nodal3.1 protein, Xenopus; C512785:wnt11b protein, Xenopus