Optimised method for determination of ticlopidine in serum by high performance liquid chromatography.
In this study we verified whether high performance liquid chromatography with UV detection (HPLC-UV) could be optimised and hence become a useful method for measuring ticlopidine concentrations in patient blood. The extraction step was improved by adding isoamylic alcohol in the extraction mixture, resulting in a better recovery. Moreover, the extraction efficiency was consistently ameliorated by evaporation to dryness of the organic extract; we could therefore utilise a lower (213 nm) wavelength, corresponding to the maximum of absorption, achieving a better sensitivity. With these technical improvements, the limit of quantitation is 0.02 microgram/ml, and the limit of detection is 0.01 microgram/ml, hence comparable to levels obtained with gas-liquid chromatography with nitrogen detection (GC-NPD). In addition, HPLC method, if compared with GC-NPD, is simpler and can be easily used for routine determinations of a lot of serum samples.
Furlan
G
G
Laboratorio di Tossicologia, Istituto di Medicina Legale e delle Assicurazioni, Ospedale Maggiore, Trieste.
Malusà
N
N
Strohmayer
A
A
Klugmann
F B
FB
Decorti
G
G
Candussio
L
L
Klugmann
S
S
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010975:Platelet Aggregation Inhibitors / Q000097:blood*; D015203:Reproducibility of Results; D013988:Ticlopidine / Q000097:blood*
D010975:Platelet Aggregation Inhibitors; D013988:Ticlopidine