PAF and haematopoiesis: IX. Platelet-activating factor increases DNA synthesis in human bone marrow cells.
Platelet-activating factor (PAF) is present in human bone marrow leading us to investigate its effect on human bone marrow cell proliferation. While PAF (0.1 microM to 1 nM) stimulates the incorporation of [3H]thymidine by freshly isolated adherent human bone marrow cells, PAF has no effect on non adherent cells. A non-metabolizable PAF agonist is more potent than PAF to stimulate thymidine incorporation in adherent cells. The precise role of PAF in human haematopoiesis in vivo remains to be clarified.
Denizot
Y
Y
Laboratoire d'Hématologie Expénmentale, Faculté de Médecine, Limoges, France.
Dupuis
F
F
Trimoreau
F
F
Verger
C
C
Allegraud
A
A
Praloran
V
V
D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D010972:Platelet Activating Factor; D014316:Tritium; D004247:DNA; D013936:Thymidine