Identification and analysis of the chicken CD3epsilon gene.
The chicken T cell receptor CD3epsilon gene was isolated using a degenerate polymerase chain reaction. The 1883 bp long cDNA encoded a transmembrane protein of 16.9 kDa lacking N-linked glycosylation sites. Comparison of the chicken and mammalian CD3epsilon proteins revealed low homology in the extracellular domain with clusters of similarities located around the N-terminal cysteine residue and proximal to the transmembrane region. The high conservation of the cytoplasmic domain included motifs important for signal transduction. The alignment of all CD3gamma, CD3delta and CD3epsilon proteins allowed the identification of highly conserved residues and motifs. Southern blot analysis indicated the presence of a single copy CD3epsilon gene. The expression of the CD3epsilon transcript was limited to T cells and natural killer cells. A recessive mutation of the CD3epsilon gene in the CB chicken strain enabled the mapping of the epitope recognized by the CT3 monoclonal antibody. This analysis of the first non-mammalian CD3epsilon gene provides novel information about evolutionary conserved structural features and its expression in natural killer cells.
Göbel
T W
TW
Basel Institute for Immunology, Switzerland. goebel@bii.ch
Fluri
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017252:CD3 Complex / Q000235:genetics*; D002645:Chickens / Q000235:genetics / Q000276:immunology; D005796:Genes; D007694:Killer Cells, Natural / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000502:physiology*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D008562:Membrane Glycoproteins