Regulation of hyaluronate metabolism by progesterone in cultured fibroblasts from the human uterine cervix.
The effects of progesterone, dehydroepiandrosterone sulfate and 17beta-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium. The changes in the radioactivity of [3H]hyaluronate showed that progesterone suppressed hyaluronate synthesis by 22% of the control levels, while dehydroepiandrosterone sulfate and 17beta-estradiol enhanced it by 22% and 12% of the control levels, respectively. Furthermore, progesterone induced degradation of high-molecular-weight [14C]hyaluronate into low-molecular-weight hyaluronate (Mr approximately 40000). These results suggest that in cultured fibroblasts from the human uterine cervix progesterone converts hyaluronate metabolism from the synthesis phase to the degradation phase.
Tanaka
K
K
Department of Biochemistry, Hirosaki University School of Medicine, Japan.
Nakamura
T
T
Takagaki
K
K
Funahashi
M
M
Saito
Y
Y
Endo
M
M
D002478:Cells, Cultured; D002584:Cervix Uteri / Q000187:drug effects / Q000378:metabolism*; D019314:Dehydroepiandrosterone Sulfate / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis / Q000378:metabolism*; D007700:Kinetics; D011374:Progesterone / Q000494:pharmacology*; D011865:Radioisotope Dilution Technique; D014316:Tritium
D014316:Tritium; D011374:Progesterone; D004958:Estradiol; D019314:Dehydroepiandrosterone Sulfate; D006820:Hyaluronic Acid; D005944:Glucosamine