The protein phosphatase inhibitor okadaic acid inhibits exit from metaphase II in parthenogenetically activated pig oocytes.
The exposure of in vitro matured pig oocytes to the calcium ionophore A 23187 (50 microM, 7 min) resulted in parthenogenetic activation in 67% of the oocytes. When the activated oocytes were cultured, they formed pronuclei. In these oocytes, tubulin labelling revealed a rearrangement of the microtubules into an interphase meshwork. The activated oocytes also lost their ability to form cytoplasmic asters after short-term taxol treatment. The activation rate of the oocytes was further increased when they were cultured with a protein synthesis inhibitor, cycloheximide, after ionophore treatment. A culture of ionophore-treated oocytes with okadaic acid, the inhibitor of protein phosphatases 1 and 2A, prevents the events characterizing oocyte activation. In oocytes cultured with okadaic acid, chromatin remained condensed, and cytoplasm retained its ability to respond to taxol treatment by the formation of cytoplasmic asters. This effect of okadaic acid was observed even in oocytes in which the activating stimulus was followed by a culture with cycloheximide. This data allows us to conclude that protein phosphatases 1 and 2A play an important role during the transition from metaphase II to interphase after activation of the pig oocyte.
Grocholová
R
R
Sanatorium Pronatal, IVF Unit, Praha-Stodůlky, Czech Republic.
Petr
J
J
Rozinek
J
J
Jílek
F
F
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D008677:Metaphase; D019319:Okadaic Acid / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D017239:Paclitaxel / Q000494:pharmacology; D010312:Parthenogenesis; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D013552:Swine; D014404:Tubulin / Q000032:analysis
D004791:Enzyme Inhibitors; D014404:Tubulin; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases; D017239:Paclitaxel