Isolation and phenotypic characterization of CD117-positive cells.
Mononuclear cells derived from cord blood were stained using the CD1 17-specific, fluorochrome-labeled monoclonal mouse antibody 95C3. Additional staining was performed using an isotype-specific rat-anti-mouse antibody, labeled with supermagnetic microparticles. Target cells were enriched by the technique of magnetic cell separation, MACS. The resulting cell population contained 96.5% (+/-1.7% S.D.) CD1 17-expressing cells (n = 12) with different levels of CD117 antigen expression. Using flow cytometry, two cell populations differing in size were found. A majority (93%) of cells with high forward scatter revealed a phenotype positive for CD117 and CD34. Isolated cells revealed a high fraction of hematopoietic progenitors (16%). The technique presented allows for an alternative approach of stem cell enrichment and might be useful in autologous transplantation of cells with hematopoietic properties.
Neu
S
S
Children's Hospital, University of Tübingen, Germany.
Geiselhart
A
A
Kuçi
S
S
Baur
F
F
Niethammer
D
D
Handgretinger
R
R
D018952:Antigens, CD34; D002469:Cell Separation / Q000379:methods*; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000145:classification*; D006801:Humans; D018189:Immunomagnetic Separation; D016130:Immunophenotyping; D007231:Infant, Newborn; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis*; D013194:Staining and Labeling
D018952:Antigens, CD34; D019009:Proto-Oncogene Proteins c-kit