Matrix metalloproteinases-1 and -8 and TIMP-1 mRNA levels in normal and diseased human gingivae.
Interstitial collagenases, including matrix metalloproteinase-1 (MMP-1) and -8 (MMP-8), serve as initiators of extracellular matrix destruction in periodontal disease. Collagenase activities are mainly regulated by tissue inhibitors of metalloproteinases (TIMPs). We tested the effects of inflammation on MMP-1 and MMP-8 gene expression in periodontal disease. To determine the relative abundance of these mRNAs in gingiva, we used a reverse transcription-polymerase chain reaction (RT-PCR) assay. Gingival biopsies were divided into 2 groups; a control group and an inflamed group with severe gingivitis or periodontitis. The MMP-1 mRNA levels were significantly elevated in inflamed gingiva, while the levels of the MMP-8 transcript were not different in the 2 groups and barely detectable by RT-PCR assay. The expression of the TIMP-1 gene was not altered, and remained higher than any of these other genes in both control and diseased gingivae. These results suggest that MMP-1 rather than MMP-8 may play an important role in the initiation of collagen degradation in periodontal disease. However, the possibility remains that MMP-8 plays an important role in periodontal tissue destruction, since the mRNA abundance and not the enzyme activity was assessed.
Aiba
T
T
Department of Biochemistry, Ohu University School of Dentistry, Koriyama, Japan.
Akeno
N
N
Kawane
T
T
Okamoto
H
H
Horiuchi
N
N
D000328:Adult; D016022:Case-Control Studies; D017364:Collagenases / Q000096:biosynthesis*; D015342:DNA Probes; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005881:Gingiva / Q000201:enzymology*; D005891:Gingivitis / Q000201:enzymology*; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1; D020784:Matrix Metalloproteinase 8; D061965:Matrix Metalloproteinase Inhibitors; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011480:Protease Inhibitors / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D018709:Statistics, Nonparametric; D019714:Tissue Inhibitor of Metalloproteinases
D015342:DNA Probes; D006023:Glycoproteins; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D017364:Collagenases; D020784:Matrix Metalloproteinase 8; D020781:Matrix Metalloproteinase 1