One-step optimization using touchdown and stepdown PCR.
Roux
K H
KH
Department of Biological Science, Florida State University, Tallahassee, USA.
Hecker
K H
KH
D001483:Base Sequence; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase; D013698:Templates, Genetic
D017931:DNA Primers; D007202:Indicators and Reagents; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase