Identification of two new mu-adaptin-related proteins, mu-ARP1 and mu-ARP2.
We report the cDNA cloning, primary structure and tissue distribution of two new proteins homologous to mu-adaptins, the medium chains of the clathrin coat adaptor complexes. Both predicted proteins share 60% amino acid sequence identity with each other and 27-31%, identity with mu1-adaptin (ap47) and mu2-adaptin (ap50). Lower similarity (23-25% identity) is found with two other mu-adaptin-related proteins, p47A/B, and there is similarity over the N-terminal 150 amino acids with the adaptin small chains and deltaCOP. The mRNAs of both molecules are expressed in all tissues analyzed, but with different profiles of relative abundance. mu-ARP1 is most abundant in brain, ovary and lung, whereas mu-ARP2 is prominently expressed in testis. These proteins suggest the existence of as yet uncharacterized types of clathrin- or non-clathrin-associated protein coats in cellular membrane traffic, of which they are probably prototype subunits, and provide molecular markers and probes for their characterization.
Wang
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X
Institut fur Physiologische Chemie, Medizinische Fakultät, Ruhr-Universität Bochum, Germany.
Kilimann
M W
MW
D033961:Adaptor Protein Complex 1; D033983:Adaptor Protein Complex mu Subunits; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002645:Chickens; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D008168:Lung; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D013737:Testis / Q000378:metabolism
D033961:Adaptor Protein Complex 1; D033983:Adaptor Protein Complex mu Subunits; C499063:Ap1m1 protein, mouse; D018076:DNA, Complementary; D008565:Membrane Proteins; C103903:mu-adaptin-related protein 2