title: Characterization of the TCRB chain repertoire in the New World monkey Callithrix jacchus.
abstract: Callithrix jacchus is an outbred New World primate characterized by a naturally occurring bone marrow chimerism, restricted polymorphism at many MHC loci, and unusual susceptibility to viral pathogens, adenocarcinoma, colitis, and, following immunization with myelin antigens, a demyelinating disease of the central nervous system closely resembling human multiple sclerosis. Here we characterize the TCRB repertoire in this species, representing the first such analysis in a New World monkey. Two TCRBC, 13 BJ, 2 BD, and 15 BV genes were identified. Overall, a high degree of similarity with human TCRBV-D-J-C gene sequences was observed, indicating a close phylogenetic relationship. Biased usage in favor of genes from the TCRBC1-BJ1 cluster was present in 77% of sequences, in contrast to preferential usage of BC2-BJ2 genes known to occur in humans and mice. Complementarity-determining region 3 averaged 10 amino acids in length and were diverse. Framework regions of TCRBV genes were extensively conserved. Phylogenetic analysis of TCRBV sequences from different species indicated that TCR genes are highly stable across primates. Thus, a diverse TCRB repertoire is generated in C. jacchus despite the limited polymorphism of class I MHC loci. Extensive homology to human TCR genes, natural chimerism, and susceptibility to inflammatory disorders are characteristics of C. jacchus that create a useful model system for the study of human autoimmunity.
authors.affiliation: Department of Neurology, University of California, San Francisco 94143, USA.
authors.forename: A
authors.initials: A
authors.lastname: Uccelli
authors.forename: J R
authors.initials: JR
authors.lastname: Oksenberg
authors.forename: M C
authors.initials: MC
authors.lastname: Jeong
authors.forename: C P
authors.initials: CP
authors.lastname: Genain
authors.forename: T
authors.initials: T
authors.lastname: Rombos
authors.forename: E E
authors.initials: EE
authors.lastname: Jaeger
authors.forename: D
authors.initials: D
authors.lastname: Giunti
authors.forename: J S
authors.initials: JS
authors.lastname: Lanchbury
authors.forename: S L
authors.initials: SL
authors.lastname: Hauser
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002144:Callithrix / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011323:Primates / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018076:DNA, Complementary; D016693:Receptors, Antigen, T-Cell, alpha-beta