title: Characterization of anti-sperm antibodies and their coding cDNA sequences by Epstein-Barr virus transformed B cell lines from lymphocytes of infertile women possessing anti-sperm antibodies.
abstract: Epstein-Barr virus (EBV)-transformed B cell lines that produce human antisperm antibodies were established using peripheral blood lymphocytes (PBLs) from infertile women with sperm immobilizing antibodies in their sera. We obtained three stable cell populations (designated B1, B2, D5) of transformed PBLs originating from three different patients. They produced IgM sperm-reacting antibodies directed against the tail of live, methanol-fixed and NaIO4-treated human spermatozoa. The established antisperm antibodies recognized noncarbohydrate sperm membrane antigens with different specificity and distribution in the male reproductive system. Antisperm antibody-B2 corresponding antigen appears to be specific for the male reproductive system. This antigen is excreted from the epithelial cells of the ductus epididymidis and bound to the spermatozoa in the lumen of the ductus. Antisperm antibodies B1 and D5 corresponding antigens were expressed on the spermatozoa in the seminiferous tubules and were common to the secretions of the ductus epididymidis, prostate and some other somatic organs. The cDNA of the immunoglobulin heavy chain genes were analyzed by reverse transcription polymerase chain reaction (RT-PCR) using RNA extracted from these clones. The immunoglobulin heavy chain cDNA sequences of these antisperm antibodies showed extremely high homology to previously reported immunoglobulin germline DNA sequences, implying that these antisperm antibodies might be natural autoantibodies rather than antibodies stimulated by external antigen.
authors.affiliation: Department of Obstetrics and Gynecology, Hyogo College of Medicine, Japan.
authors.forename: D K
authors.initials: DK
authors.lastname: Dimitrova-Dikanarova
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuji
authors.forename: Y
authors.initials: Y
authors.lastname: Nakata
authors.forename: H
authors.initials: H
authors.lastname: Shibahara
authors.forename: M
authors.initials: M
authors.lastname: Mitsuo
authors.forename: T
authors.initials: T
authors.lastname: Hashimoto
authors.forename: J
authors.initials: J
authors.lastname: Furuyama
authors.forename: K
authors.initials: K
authors.lastname: Koyama
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D001483:Base Sequence; D002461:Cell Line, Transformed / Q000821:virology; D002472:Cell Transformation, Viral; D018076:DNA, Complementary / Q000737:chemistry*; D000939:Epitopes / Q000378:metabolism; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D005803:Genes, Immunoglobulin; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D007247:Infertility, Female / Q000276:immunology*; D008297:Male; D008969:Molecular Sequence Data; D013081:Sperm Motility / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D018076:DNA, Complementary; D000939:Epitopes