title: Trace analysis of albendazole and its sulphoxide and sulphone metabolites in milk by liquid chromatography.
abstract: Analytical methodology for determination of albendazole and its sulphoxide and sulphone metabolites in milk at levels down to 2-5 ng/ml has been developed. Extraction was carried out with ethyl acetate under alkaline conditions, and extracts were analyzed on a silica-based C18 column in the presence of positively-charged pairing ions. Accuracy data showed overall recoveries ranged from 78.4% to 100%, whereas precision data, based on within and between-day variation, suggested overall precision values better than 4.9%. The method was successfully applied to determine residues in milk of a dairy cow orally given albendazole.
authors.affiliation: Laboratory of Milk Hygiene and Technology, School of Veterinary Medicine, Aristotle University, Thessoloniki, Greece.
authors.forename: D J
authors.initials: DJ
authors.lastname: Fletouris
authors.forename: N A
authors.initials: NA
authors.lastname: Botsoglou
authors.forename: I E
authors.initials: IE
authors.lastname: Psomas
authors.forename: A I
authors.initials: AI
authors.lastname: Mantis
mesh_terms: D015766:Albendazole / Q000031:analogs & derivatives / Q000032:analysis*; D000818:Animals; D000871:Anthelmintics / Q000032:analysis; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D005240:Feasibility Studies; D005260:Female; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013450:Sulfones / Q000032:analysis; D013454:Sulfoxides / Q000032:analysis
chemical_list: D000871:Anthelmintics; D013450:Sulfones; D013454:Sulfoxides; C027187:albendazole sulfone; D015766:Albendazole; C027186:albendazole sulfoxide