Inhibition of peritoneal dissemination of colon carcinoma in syngeneic mice immunized with interleukin-2-producing cells.
We have examined the antitumor effect of murine colon carcinoma cells engineered to produce human interleukin-2 (IL-2) in syngeneic mice. Subcutaneous inoculation of retrovirally-transduced cells with IL-2 gene formed small tumors, but they became regressed spontaneously. Consequently, the inoculated mice showed prolonged survival. Histological examination of the tumors derived from IL-2-producers revealed predominant infiltration of macrophages around tumor necrotic masses. Thus, inoculation of IL-2-producing cells could protect the mice from subsequent subcutaneous or intraperitoneal challenges with wild-type cells, suggesting the induction of acquired immunity due to the effect of tumor vaccination.
Gunji
Y
Y
Department of Surgery (II), School of Medicine, Chiba University, Japan.
Tagawa
M
M
Matsubara
H
H
Takenaga
K
K
Sugaya
M
M
Tasaki
K
K
Maeda
T
T
Kondo
F
F
Nakajima
K
K
Suzuki
T
T
Asano
T
T
Ochiai
T
T
Isono
K
K
Sakiyama
S
S
D000818:Animals; D003106:Colon / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D010534:Peritoneal Neoplasms / Q000276:immunology / Q000517:prevention & control*; D012190:Retroviridae / Q000235:genetics
D007376:Interleukin-2