title: Expression of mRNA encoding intestinal type alkaline phosphatase in rat liver and its increase by fat-feeding.
abstract: The presence of types of alkaline phosphatase (ALP) other than the tissue non-specific type enzyme in rat liver and its increase by fat feeding are known. In order to examine expression of intestinal type ALP in liver, specific oligonucleotide primers corresponding to two types of mRNAs of rat intestinal ALP (RTIN-1 and -2) were designed and amplified by means of the reverse transcriptase-polymerase chain reaction (RT-PCR). It was found that RTIN-1 mRNA was expressed only in the intestine but not in the liver, while RTIN-2 mRNA was expressed both in the intestine and in the liver. By fat feeding, expression of RTIN-1 mRNA increased in the intestine and that of RTIN-2 mRNA increased both in the intestine and in the liver. Thus, it was concluded that rat liver expressed one of the intestinal type ALP (RTIN-2) which was enhanced by fat feeding.
authors.affiliation: Department of Biochemistry, Tokyo Medical and Dental University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Goseki-Sone
authors.forename: S
authors.initials: S
authors.lastname: Oida
authors.forename: T
authors.initials: T
authors.lastname: Iimura
authors.forename: A
authors.initials: A
authors.lastname: Yamamoto
authors.forename: H N
authors.initials: HN
authors.lastname: Matsumoto
authors.forename: N
authors.initials: N
authors.lastname: Omi
authors.forename: K
authors.initials: K
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Maruoka
authors.forename: I
authors.initials: I
authors.lastname: Ezawa
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D000818:Animals; D001483:Base Sequence; D004041:Dietary Fats / Q000008:administration & dosage*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007422:Intestines / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004041:Dietary Fats; D012333:RNA, Messenger; D000469:Alkaline Phosphatase