Fluorescence detection of specific sequence of nucleic acids by oxazole yellow-linked oligonucleotides. Homogeneous quantitative monitoring of in vitro transcription.
We have developed a fluorescent DNA probe, oxazole yellow (YO)-linked oligonucleotide complementary to a target DNA/RNA, which can enhance the fluorescence on hybridizing with a target nucleotide. We demonstrated the applicability of the YO-linked oligonucleotide probe to real-time monitoring of the in vitro transcription process of a plasmid DNA constructed containing the 5'-terminus non-coded region of hepatitis C virus RNA. In the process of in vitro transcription in the presence of YO-linked complementary oligonucleotide, the fluorescence of the reaction mixture showed a time-dependent linear increase corresponding to the generated target RNA product.
Ishiguro
T
T
Tokyo Research Laboratories, Tosoh Corporation, Ayase-shi, Kanagawa, Japan. isiguro@tosoh.co.jp
Saitoh
J
J
Yawata
H
H
Otsuka
M
M
Inoue
T
T
Sugiura
Y
Y
D001583:Benzoxazoles / Q000737:chemistry*; D001665:Binding Sites; D015342:DNA Probes; D005456:Fluorescent Dyes; D009696:Nucleic Acids / Q000737:chemistry / Q000235:genetics*; D011804:Quinolines / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D001583:Benzoxazoles; D015342:DNA Probes; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011804:Quinolines; C097015:oxazole yellow