title: A simple and rapid nonisotopic method for sizing CAG repeats in the SCA1 gene.
abstract: Spinocerebellar ataxia type 1 is caused by the expansion of a CAG trinucleotide repeat, located at the 5' end of the gene responsible for the disease (SCA1 gene). We propose a simple and rapid method for SCA1 diagnosis, avoiding both radioactive and Southern blotting analysis. The method allows an accurate allele sizing by visualization of polymerase chain reaction products through a silver nitrate-stained polyacrylamide gel.
authors.affiliation: Istituto di Medicina Sperimentale e Biotecnologie, CNR, Cosenza, Italia.
authors.forename: G
authors.initials: G
authors.lastname: Annesi
authors.forename: M
authors.initials: M
authors.lastname: Muglia
authors.forename: F L
authors.initials: FL
authors.lastname: Conforti
authors.forename: O
authors.initials: O
authors.lastname: Leone
authors.forename: C
authors.initials: C
authors.lastname: Grandinetti
authors.forename: E
authors.initials: E
authors.lastname: Imbrogno
authors.forename: A L
authors.initials: AL
authors.lastname: Gabriele
authors.forename: F
authors.initials: F
authors.lastname: Naso
authors.forename: C
authors.initials: C
authors.lastname: Brancati
mesh_terms: D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D006801:Humans; D013132:Spinocerebellar Degenerations / Q000235:genetics*; D018911:Trinucleotide Repeats / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D004262:DNA Restriction Enzymes