[Cyanocobalamin Reference Standard (Control 951) of the National Institute of Health Sciences].
The raw material of cyanocobalamin was tested for preparation of the "Cyanocobalamin Reference Standard (Control 951)". Analytical data obtained are as follows: loss on drying, 1.8%; infrared spectrum, the same as that of the JP Cyanocobalamin Reference Standard (Control 936); thin-layer chromatography, three impurities were detected; high-performance liquid chromatography (HPLC), eight to nine kinds of impurities were detected and the total amount of impurities was estimated to be 1.6 +/- 0.13% (n = 4); assay, 99.7% by spectrophotometry specified in the JP XII and 100.4% by HPLC, respectively. Based on the above results, the raw material was authorized as the JP Cyanocobalamin Reference Standard (Control 951).
Kitajima
A
A
Maekawa
K
K
Yoshii
K
K
Komatsu
H
H
Tanimoto
T
T
Okada
S
S
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006077:Government Agencies; D007564:Japan; D010602:Pharmacopoeias as Topic / Q000592:standards; D012015:Reference Standards; D014805:Vitamin B 12 / Q000032:analysis / Q000592:standards*
D014805:Vitamin B 12