In vivo photocycle of the Euglena gracilis photoreceptor.
We present the light-induced photocycle of the paraflagellar swelling of Euglena gracilis. The kinetics of this process was reconstructed by sampling its fluorescence emission and switching the excitation light from 365 nm to 436 nm. Stable intermediates in the photocycle were manifested. The measured millisecond resolution kinetics best fits a Michaelis-Menten equation. The data provide strong evidence that the paraflagellar swelling, a three-dimensional natural crystal of a light-detecting protein, is the true Euglena photoreceptor.
Barsanti
L
L
Istituto di Biofisica, CNR, Pisa, Italy.
Passarelli
V
V
Walne
P L
PL
Gualtieri
P
P
D000818:Animals; D005056:Euglena gracilis / Q000378:metabolism* / Q000648:ultrastructure; D005407:Flagella / Q000378:metabolism / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D007700:Kinetics; D008027:Light; D008856:Microscopy, Fluorescence; D018462:Photobiology; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism
D012243:Rhodopsin