title: Expression of the nucleocapsid protein gene of the canine distemper virus.
abstract: We constructed a cDNA clone of canine distemper virus (CDV) encoding an entire nucleoprotein (NP) gene, by means of the reverse transcription-polymerase chain reaction (RT-PCR). The cloned NP gene was inserted into the eucaryotic expression vector, pRVSV. After transfection of the plasmid into Vero cells, we examined the expression of CDV-specific NP antigen by means of indirect immunofluorescence assay (IFA) and Western blotting, using various antibodies against NP of CDV and an antiserum against NP of measles virus. The CDV-NP specific antigen was detected in the nuclei of the cells transfected with pRV-ON, by means of IFA with antibodies specific to the NP.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: Y S
authors.initials: YS
authors.lastname: Shin
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Tomonaga
authors.forename: K
authors.initials: K
authors.lastname: Iwatsuki
authors.forename: C
authors.initials: C
authors.lastname: Kai
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004217:Distemper Virus, Canine / Q000235:genetics* / Q000378:metabolism*; D004285:Dogs; D008969:Molecular Sequence Data; D019251:Nucleocapsid / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D014709:Vero Cells
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins