title: Novel group within the kingdom Crenarchaeota from boreal forest soil.
abstract: We report here the results of phylogenetic analysis of archaeal 16S rRNA gene sequences amplified by PCR with Archaea-specific primers with mixed-population DNA extracted directly from forest soil used as a template. Nucleotide signature and phylogenetic analyses show that the sequences obtained belong to the domain Archaea and form a new cluster. Its phylogenetic position suggests that sequences are from a previously undescribed terrestrial group within the kingdom Crenarchaeota.
authors.affiliation: Department of Applied Chemistry and Microbiology, Helsinki University, Finland. german.jurgens@helsinki.fi
authors.forename: G
authors.initials: G
authors.lastname: Jurgens
authors.forename: K
authors.initials: K
authors.lastname: Lindström
authors.forename: A
authors.initials: A
authors.lastname: Saano
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D005387:Finland; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S