Molecular cloning of ERp29, a novel and widely expressed resident of the endoplasmic reticulum.
We have isolated a full-length cDNA clone for a novel 29 kDa protein that is highly expressed in rat enamel cells. The clone encodes a 259-residue protein, here named ERp29, with structural features (signal peptide and a variant endoplasmic reticulum-retention motif, KEEL) that indicate it is a reticuloplasmin. ERp29 has limited homology with protein disulfide isomerase and its cognates, but lacks their characteristic thioredoxin-like catalytic moiety and calcium-binding motifs. ERp29 mRNA was expressed in all rat tissues tested, and a homologous transcript was detected in other animal livers (primate, ruminant, marsupial). In human hepatoma cells, ERp29 mRNA expression was not increased by stresses (tunicamycin, calcium ionophore) that induced other reticuloplasmins. We conclude that ERp29 is a new, highly conserved member of the reticuloplasmin family which is widely expressed. The apparent lack of both calcium binding properties and stress responsiveness distinguish ERp29 from all major reticuloplasmins characterised to dates.
Demmer
J
J
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Zhou
C
C
Hubbard
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D003743:Dental Enamel / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006801:Humans; D007535:Isomerases / Q000096:biosynthesis* / Q000737:chemistry; D008099:Liver / Q000378:metabolism*; D008394:Marsupialia; D008963:Molar; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011323:Primates; D019704:Protein Disulfide-Isomerases; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012418:Ruminants; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D015342:DNA Probes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007535:Isomerases; D019704:Protein Disulfide-Isomerases