The effect of space flight on monoclonal antibody synthesis in a hybridoma mouse cell line.
The hybridoma cell line, 3G10G5, producing a monoclonal antibody to the major capsid protein VP1 from the avian polyomavirus budgerigar fledgling disease virus, was produced from a Balb/C mouse. This cell line was used to test the effects of microgravity on cellular processes, specifically protein synthesis. A time course study utilizing incorporation of [35S]methionine into newly synthesized monoclonal antibody was performed on STS-77. After 5.5 days, it was observed that cell counts for the samples exposed to microgravity were lower than those of ground-based samples. However, radiolabel incorporation of the synthesized monoclonal antibody was similar in both orbiter and ground control samples. Overall, microgravity does not seem to have an effect on this cell line's ability to synthesize IgG protein.
Smiley
S A
SA
Division of Biology--Ackert Hall, Section of Virology and Oncology, Kansas State University, Manhattan 66506, USA.
Gillock
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ET
Black
M C
MC
Consigli
R A
RA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D006825:Hybridomas / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D013026:Space Flight; D018561:Space Simulation; D014407:Tumor Cells, Cultured; D014893:Weightlessness
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus