title: Fibroblast growth factor-2 (FGF-2) and FGF-receptor (FGFR-1) immunoreactivity in embryonic spinal autonomic neurons.
abstract: The development of the nervous system appears to be under the control of multiple growth factors, neurotrophins and cytokines, which may be expressed either continuously or transiently throughout defined stages of cellular generation, proliferation or differentiation. Fibroblast growth factor (FGF) cytokines and their receptors are abundantly expressed in the embryonic nervous system but their localization at autonomic levels in the fetal spinal cord has not yet been detailed. Immunoreactivity to FGF-2, probably the best characterized member of the FGF family (FGF-1 to FGF-10) and of one of its high affinity receptors, FGFR-1, was found in autonomic neurons at embryonic day E14, the peak day of generation and proliferation in the common ventral motoneuron pool. It was also continuously present throughout the investigated subsequent stages (E15 to postnatal day P30). Immunogold electron microscopy revealed the cytoplasmic localization of FGF-2 and FGFR-1 in intermediolateral neurons, the major group of sympathetic preganglionic neurons in the spinal cord. In these neurons, immunocytochemistry from E14 onwards showed the co-distribution of both markers at the period of axonal outgrowth to peripheral targets, e.g. the adrenal medulla. Our findings suggest autocrine and/or paracrine actions of FGF-2 for sympathetic preganglionic development but do not support its role as a target-derived neurotrophic factor for autonomic neuron development.
authors.affiliation: Department of Neurology, University Clinics Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, D-12200 Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Stapf
authors.forename: G
authors.initials: G
authors.lastname: Lück
authors.forename: M
authors.initials: M
authors.lastname: Shakibaei
authors.forename: D
authors.initials: D
authors.lastname: Blottner
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000918:Antibody Specificity; D001341:Autonomic Nervous System / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development; D015153:Blotting, Western; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D016253:Microscopy, Immunoelectron; D009474:Neurons / Q000737:chemistry* / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000032:analysis / Q000276:immunology*; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D013347:Subcellular Fractions / Q000737:chemistry
chemical_list: D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; C496350:Fgfr1 protein, rat; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1