Tape stripping induces marked epidermal proliferation and altered TGF-alpha expression in non-lesional psoriatic skin.
Psoriasis is a chronic inflammatory disorder characterized by epidermal hyperproliferation. Although recent evidence suggests that T cell activation is a primary trigger for psoriasis lesions, there may be alterations in the keratinocyte growth regulatory pathways which induce epidermal hyperproliferation in psoriatic patients. To test this hypothesis, we investigated the proliferative activity of epidermal keratinocytes 48 h after tape stripping, one of the standard mechanical ways to stimulate the epidermis, in 20 psoriasis patients and in 18 controls. Epidermal cell kinetics were assessed with DNA flow cytometry, the mitotic index, bromodeoxyuridine incorporation, Ki-67 antigen expression and DNA polymerase alpha expression. The expression of TGF-alpha and EGF receptors, critical mediators of keratinocyte proliferation, were also investigated immunohistochemically. The results of multiparameter assays showed that the baseline proliferative activity in uninvolved skin was the same in psoriasis patients and normal controls. After tape stripping, although both psoriasis patients and the normal controls showed significant increases in epidermal cell proliferation, the values of all the parameters investigated were significantly greater in the psoriasis patients than in the normal controls. EGF receptors were overexpressed in basal and suprabasal keratinocytes after tape stripping in both the psoriasis patients and the normal controls. In contrast, overexpression of TGF-alpha was only observed in the patients with psoriasis, which may explain their increased proliferative response to trauma.
Hatta
N
N
Department of Dermatology, Kanazawa University School of Medicine, Takara-machi, Japan.
Takata
M
M
Kawara
S
S
Hirone
T
T
Takehara
K
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001973:Bromodeoxyuridine / Q000378:metabolism; D016022:Case-Control Studies; D002455:Cell Division; D004247:DNA / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008940:Mitotic Index; D010812:Physical Stimulation; D011565:Psoriasis / Q000378:metabolism* / Q000473:pathology*; D016211:Transforming Growth Factor alpha / Q000378:metabolism*
D019394:Ki-67 Antigen; D016211:Transforming Growth Factor alpha; D004247:DNA; D066246:ErbB Receptors; D004257:DNA Polymerase II; D001973:Bromodeoxyuridine