title: Ly1- (CD5-) B cells produce interleukin (IL)-10.
abstract: Interleukin (IL)-10 enhances humoral immunity by inhibiting macrophage activation and promoting the development of Th2 cytokine synthesis. In this study we investigated the ability of conventional Ly-1- (CD5-) B cells to produce IL-10 protein. Highly purified normal, naive splenic B cells from both BALB/c and C57BL/6 mice produced IL-10 in response to stimulation with the mitogen LPS. In addition, B cells from antigen primed mice also produced IL-10 upon antigen restimulation in vitro, as analyzed by ELISA and by bioassay. Removal of Ly-1+ B cells did not appreciably reduce IL-10 production, indicating that conventional Ly-1- B cells produced IL-10. These results indicate that normal Ly-1- B cells produce significant quantities of IL-10 during mitogen- or antigen-driven immune responses. The production of IL-10 by conventional B cells may enhance their capacity to promote humoral immune responses.
authors.affiliation: Department of Pediatrics, Stanford University, California 94305, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Gieni
authors.forename: D T
authors.initials: DT
authors.lastname: Umetsu
authors.forename: R H
authors.initials: RH
authors.lastname: DeKruyff
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens / Q000276:immunology*; D002469:Cell Separation; D002478:Cells, Cultured; D016753:Interleukin-10 / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000166:cytology
chemical_list: D018956:CD5 Antigens; D008070:Lipopolysaccharides; D016753:Interleukin-10; D018664:Interleukin-12; D010047:Ovalbumin