TNF can directly induce the expression of ubiquitin-dependent proteolytic system in rat soleus muscles.
Incubation of isolated rat soleus muscles in the presence of human recombinant TNF-alpha (10,000 U/ml) resulted in an important increase in ubiquitin gene expression (over 50%). Although previous studies involving cytokine administration in vivo (1) have demonstrated an action on ubiquitin-dependent proteolysis, this is the first report demonstrating a direct action of the cytokine on protein breakdown in incubated rat skeletal muscle.
Llovera
M
M
Department de Biologia Cellular, Facultat de Medicina, Universitat de Barcelona, Spain.
García-Martínez
C
C
Agell
N
N
López-Soriano
F J
FJ
Argilés
J M
JM
D000818:Animals; D003546:Cysteine Endopeptidases / Q000096:biosynthesis*; D006801:Humans; D009097:Multienzyme Complexes / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014452:Ubiquitins / Q000096:biosynthesis / Q000378:metabolism*
D009097:Multienzyme Complexes; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D014452:Ubiquitins; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex