title: An allelic series of blue fluorescent trp1 mutants of Arabidopsis thaliana.
abstract: Nine blue fluorescent mutants of the flowering plant Arabidopsis thaliana were isolated by genetic selections and fluorescence screens. Each was shown to contain a recessive allele of trp1, a previously described locus that encodes the tryptophan biosynthetic enzyme phosphoribosylanthranilate transferase (PAT, called trpD in bacteria). The trp1 mutants consist of two groups, tryptophan auxotrophs and prototrophs, that differ significantly in growth rate, morphology, and fertility. The trp1 alleles cause plants to accumulate varying amounts of blue fluorescent anthranilate compounds, and only the two least severely affected of the prototrophs have any detectable PAT enzyme activity. All four of the trp1 mutations that were sequenced are G to A or C to T transitions that cause an amino acid change, but in only three of these is the affected residue phylogenetically conserved. There is an unusually high degree of sequence divergence in the single-copy gene encoding PAT from the wild-type Columbia and Landsberg erecta ecotypes of Arabidopsis.
authors.affiliation: Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853-1801, USA. abrose@ucdavis.edu
authors.forename: A B
authors.initials: AB
authors.lastname: Rose
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: R L
authors.initials: RL
authors.lastname: Last
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000877:Anthranilate Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D018744:DNA, Plant; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005453:Fluorescence; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005808:Genes, Recessive; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012333:RNA, Messenger; D062367:ortho-Aminobenzoates / Q000378:metabolism / Q000494:pharmacology
chemical_list: D018744:DNA, Plant; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D012333:RNA, Messenger; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; C098239:6-methylanthranilic acid; D000877:Anthranilate Phosphoribosyltransferase