Determination of lincomycin residues in salmon tissues by gas chromatography with nitrogen-phosphorus detection.
A sensitive method for the determination of lincomycin residues in fish tissues is described. Lincomycin was extracted from fish tissues with phosphate buffer (pH 4.5). The extract was concentrated with a C18 solid-phase extraction cartridge and further cleaned up by solvent extraction. Lincomycin was derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide to form a trimethylsilyl derivative before being analyzed by gas chromatography with nitrogen-phosphorus detection. Coumaphos was used as the internal standard. Assays showed good linearity in the range 25-250 ppb (ng/g) (r = 0.9994). Recoveries of fortified lincomycin at 50, 100 and 200 ppb were > 80% with relative standard deviations < 6%. The limit of detection of the method was 1.7 ppb and the limit of quantitation was 3.8 ppb.
Luo
W
W
Department of Health and Human Services, Public Health Service, Food and Drug Administration, Jefferson, AR 72079-9502, USA.
Yin
B
B
Ang
C Y
CY
Rushing
L
L
Thompson
H C
HC
D000081:Acetamides; D000818:Animals; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D008034:Lincomycin / Q000032:analysis*; D009584:Nitrogen / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D012474:Salmon; D012680:Sensitivity and Specificity; D014297:Trimethylsilyl Compounds
D000081:Acetamides; C103255:N,O-bis(trimethylsilyl)trifluoroacetamide; D014297:Trimethylsilyl Compounds; D010758:Phosphorus; D008034:Lincomycin; D009584:Nitrogen