Characteristics of a developmental arrestant in the venom of the ectoparasitoid wasp Euplectrus comstockii.
Parasitic Hymenoptera regulate their hosts in order to provide a suitable source of nutrition and dwelling for their offspring. Few regulatory factors known to cause a specific effect on the host have been structurally characterized. The larval ectoparasitoid Euplectrus comstockii Howard (Hymenoptera: Eulophidae) arrests larval-larval ecdysis in its lepidopteran hosts. Prior to oviposition, the female wasp inject a venom into the hemocoel of the host and that venom alone is effective in causing the arrestment. A venom gland-reservoir structure connected to the lower reproductive tract of the wasp contains a complex mixture of proteins. There are no obvious similarities among the electrophoretic banding pattern (native or denatured) for venom proteins of E comstockii and several other parasitic hymenopteran species. Venomous protein, separated by electrophoretic techniques, with a native mol. wt of c. 66,000, was capable of arresting larval-larval ecdysis in 4th instar larvae of Trichoplusia ni (Lepidoptera: Noctuidae). Nanogram quantities of the protein were sufficient to cause arrestment. The activity of the protein was sensitive to temperature, pH, organic solvent, and protease.
Coudron
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Biological Control of Insects Research Laboratory, U. S. Department of Agriculture, Columbia, MO 65203-3535, USA.
Brandt
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D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008845:Microinjections; D009036:Moths / Q000187:drug effects / Q000254:growth & development* / Q000469:parasitology*; D014862:Wasp Venoms / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity*; D014863:Wasps
D014862:Wasp Venoms