Detection of Vibrio anguillarum by a sandwich enzyme-linked immunosorbent assay performed with monoclonal antibodies.
Monoclonal antibodies (Mabs) against V. anguillarum were produced and characterized by Western blotting analysis, competitive binding assays and cross-reactivity tests. Their ability to detect V. anguillarum in a liquid culture was tested in a sandwich enzyme-linked immunosorbent assay (ELISA) performed with different combinations of these Mabs used as capture or tracer antibodies. One combination was selected as the most suitable for diagnostic applications, showing the highest sensitivity and specificity.
Adone
R
R
Istituto Superiore di Sanità, Rome, Italy.
Piccininno
G
G
Pistoia
C
C
Bianchi
R
R
Ciuchini
F
F
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D001667:Binding, Competitive; D015153:Blotting, Western / Q000379:methods / Q000662:veterinary; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D005399:Fishes / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D014733:Vibrio / Q000276:immunology* / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal