Rapid and differential detection of two analogous enterotoxins of Vibrio cholerae and enterotoxigenic Escherichia coli by a modified enzyme-linked immunosorbent assay.
The principle of a novel ELISA (nylon-slip immuno-test, NSIT) was applied to the differential detection of two analogous enterotoxins, cholera toxin (CT) of Vibrio cholerae and heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli. The results obtained for CT and LT detection by a single test were sufficiently sensitive (87.9 and 100%) and specific (100 and 94.7%) in the differential detection test, when compared with the result of a colony hybridization test with DNA probes. The results suggest that the novel ELISA is applicable to the diagnosis of bacterial infections, by means of differential immunological detection of toxins in a single test.
Koike
N
N
Research and Development Center, Unitika Ltd., Uji, Kyoto, Japan.
Okada
K
K
Yabushita
Y
Y
Zhang
D
D
Yamamoto
K
K
Miwatani
T
T
Honda
T
T
D001427:Bacterial Toxins / Q000032:analysis*; D002771:Cholera / Q000175:diagnosis; D002772:Cholera Toxin / Q000032:analysis*; D003429:Cross Reactions; D004247:DNA / Q000032:analysis; D003937:Diagnosis, Differential; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000175:diagnosis; D029968:Escherichia coli Proteins; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000276:immunology*
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D004247:DNA; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli