Production and characterization of a new monoclonal antibody effective in recognizing the CD3 T-cell associated antigen in formalin-fixed embedded tissue.
Phenotypic analysis of lymphoproliferative disorders is now considered mandatory for accurate classification which is the basis for optimum patient management. This is presently carried out in most cases using a range of antibodies recognizing B and T-cell antigens effective in paraffin sections, and an antibody to CD 3 is currently a key member of such panels, indicating T-cell phenotype. Current antibodies to CD3 are polyclonal with the inherent disadvantages of this type of reagent compared to monoclonal antibodies. In this study, we have used a recombinant fusion protein representing part of the epsilon subunit of the CD3 molecule to generate a novel monoclonal antibody (NCL-CD3-PS1) effective in paraffin sections. The antibody has been characterized biochemically and by immunohistochemistry using a wide range of normal and pathological tissues. Lineage and phenotype specificity have been supported in our study and results from other laboratories are awaited with interest.
Steward
M
M
University Department of Pathology, Royal Victoria Infirmary, University of Newcastle upon Tyne, UK.
Bishop
R
R
Piggott
N H
NH
Milton
I D
ID
Angus
B
B
Horne
C H
CH
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry*; D017252:CD3 Complex / Q000032:analysis / Q000276:immunology*; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000473:pathology; D016612:Paraffin Embedding; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry*; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D011994:Recombinant Proteins; D005557:Formaldehyde