A Leuconostoc lactis protein with homology to ribosomal protein S1 shares common epitopes and common DNA binding properties with a mammalian DNA binding nuclear factor.
A mouse testis cDNA expression library (Clontech) was screened with a synthetic oligonucleotide ligand containing CT-rich motifs derived from the rat skeletal muscle actin gene promoter. These motifs bind nuclear proteins, and seem to be involved in the regulation of the gene. Analysis of isolated clones, which expressed proteins that specifically bind the oligonucleotide, indicated that they were derived from a single gene. This gene was identified as a contaminant of bacterial origin (Leuconostoc lactis). The cloned gene from L. lactis encodes a protein with significant homology to bacterial ribosomal protein S1, which we designated LrpS1-L. Band shift analysis and competition experiments indicated that both the bacterial protein and a mouse nuclear protein specifically bind to the same CT-rich motif of the skeletal muscle actin promoter. Furthermore, antibodies against the recombinant bacterial protein interfered with the formation of complex between the CT-rich element and the mouse nuclear protein. These results indicate that the bacterial LrpS1-L protein and the mammalian protein bind the same CT-rich motif and share common antigenic epitopes.
Yamit-Hezi
A
A
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel.
Levy
Z
Z
Neuman
S
S
Nudel
U
U
D000199:Actins / Q000235:genetics*; D000818:Animals; D000907:Antibodies, Bacterial; D019138:Antibodies, Blocking; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D000939:Epitopes / Q000276:immunology*; D015723:Gene Library; D007934:Leuconostoc / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology; D012269:Ribosomal Proteins / Q000235:genetics* / Q000276:immunology*; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D013737:Testis / Q000737:chemistry
D000199:Actins; D000907:Antibodies, Bacterial; D019138:Antibodies, Blocking; D001426:Bacterial Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D000939:Epitopes; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C015494:ribosomal protein S1