title: High-throughput microsatellite analysis using fluorescent dUTPs for high-resolution genetic mapping of the mouse genome.
abstract: The use of fluorescent end-labeled primers has proved successful for rapid, semiautomated genotyping of microsatellite loci. However, custom synthesis is expensive and costs can be prohibitive when a wide range of markers is to be analyzed for only a few genotypings. This particularly applies to high-resolution genetic mapping in the mouse either in the construction of global maps or in the production of local high-resolution genetic maps for positional cloning. We demonstrate here the use of fluorescent dUTPs for cost-effective, high-throughput microsatellite genotyping in the mouse. This alternative to the use of fluorescent end-labeled primers for semiautomated genotyping is potentially applicable to the construction of linkage maps in other species.
authors.affiliation: MRC Human Genome Mapping Project Resource Centre, Hinxton, Cambridge, UK.
authors.forename: M
authors.initials: M
authors.lastname: Rhodes
authors.forename: A
authors.initials: A
authors.lastname: Dearlove
authors.forename: R
authors.initials: R
authors.lastname: Straw
authors.forename: S
authors.initials: S
authors.lastname: Fernando
authors.forename: A
authors.initials: A
authors.lastname: Evans
authors.forename: M
authors.initials: M
authors.lastname: Greener
authors.forename: T
authors.initials: T
authors.lastname: Lacey
authors.forename: M
authors.initials: M
authors.lastname: Kelly
authors.forename: K
authors.initials: K
authors.lastname: Gibson
authors.forename: S D
authors.initials: SD
authors.lastname: Brown
authors.forename: C
authors.initials: C
authors.lastname: Mundy
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; D005819:Genetic Markers; D005838:Genotype; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; D005819:Genetic Markers