Reduced virulence of Candida albicans MKC1 mutants: a role for mitogen-activated protein kinase in pathogenesis.
Deletion of the Candida albicans mitogen-activated protein kinase MKC1 gene gave rise to viable cells whose cell integrity was affected (F. Navarro-García, M. Sánchez, J. Pla, and C. Nombela, Mol. Cell. Biol. 15:2197-2206, 1995). In an experimental infection system using a murine model, the C. albicans mkc1 delta/mkc1 delta strain was found to be less pathogenic than the parental strain, as show the different time of survival, percentage of mortality, fungal load in the most representative organs, and histological analysis. This is the first study that shows the involvement of the cell integrity pathway in the pathogenicity of a dimorphic fungus.
Diez-Orejas
R
R
Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Molero
G
G
Navarro-García
F
F
Pla
J
J
Nombela
C
C
Sanchez-Pérez
M
M
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000502:physiology*; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity*; D002177:Candidiasis / Q000201:enzymology / Q000209:etiology* / Q000382:microbiology*; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D016296:Mutagenesis; D014774:Virulence
D017871:Calcium-Calmodulin-Dependent Protein Kinases