title: Utilization of phytate phosphorus and calcium as influenced by microbial phytase, cholecalciferol, and the calcium: total phosphorus ratio in broiler diets.
abstract: The present study was performed to evaluate the potential of microbial phytase and cholecalciferol (D3) for improving the utilization of phytate P and Ca and the influence of the Car:total (t) P ratio in a corn-soybean meal diet fed to broilers from hatch to 21 d of age. A 4 x 4 x 2 factorial arrangement of treatments was used: 1.1, 1.4, 1.7, and 2.0:1 Ca:tP ratio; 0, 300, 600, and 900 U of phytase/kg of diet; and 66 and 660 micrograms of D3/kg of diet. Another four treatments were included: the four Ca:tP ratios with 6,600 micrograms of D3 addition, but without phytase. Added phytase linearly increased (P < 0.001) BW gain, feed intake, toe ash content, and P and Ca retention; these measurements were negatively influenced by widening the dietary Ca:tP ratio, and synergetically improved by addition of D3. Increasing the Ca:tP ratio decreased (P < 0.001) all measurements in the presence or absence of supplemental phytase and D3. Dietary Ca:tP ratios between 1.1:1 to 1.4:1 appears critical to the efficient use of supplemental phytase and D3 for improving the utilization of phytate P and Ca. The addition of D3 in corn-soybean meal diets indicated a potential for improving the utilization of phytate P and Ca by increasing Ca and P retention by about 5 to 12% in birds, which led to an increase in toe ash content (P < 0.03). The enhanced phytate P utilization (P < 0.001) was also observed during assay of the phytase activity in the mixed diets with an addition of D3 and without added phytase. In summary, the findings of this study suggested that phytase, D3, and Ca:tP are important factors in degrading phytate and improving phytate P and Ca utilization in broilers.
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061-0306, USA.
authors.forename: H
authors.initials: H
authors.lastname: Qian
authors.forename: E T
authors.initials: ET
authors.lastname: Kornegay
authors.forename: D M
authors.initials: DM
authors.lastname: Denbow
mesh_terms: D010832:6-Phytase / Q000494:pharmacology*; D000375:Aging / Q000378:metabolism / Q000502:physiology; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002136:Calcium, Dietary / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D002645:Chickens / Q000378:metabolism* / Q000502:physiology; D002762:Cholecalciferol / Q000494:pharmacology*; D004032:Diet / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D008297:Male; D008903:Minerals / Q000494:pharmacology; D010758:Phosphorus / Q000378:metabolism*; D016226:Phosphorus, Dietary / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D011897:Random Allocation; D013025:Glycine max / Q000592:standards; D015430:Weight Gain / Q000187:drug effects / Q000502:physiology; D003313:Zea mays / Q000592:standards
chemical_list: D002136:Calcium, Dietary; D008903:Minerals; D016226:Phosphorus, Dietary; D002762:Cholecalciferol; D010758:Phosphorus; C028705:bone ash; D010832:6-Phytase; D002118:Calcium