title: Use of Chemically Modified Silica with beta-Diketoamine Groups for Separation of alpha-Lactoalbumin from Bovine Milk Whey by Affinity Chromatography
abstract: Silica gel surface was chemically modified with beta-diketoamine groups by reacting the silanol from the silica surface with 3-aminopropyl-triethoxysilane and 3-bromopentanedione. With this material, copper ions were adsorbed from aqueous solutions. The chemical analysis of the silica-gel-immobilized acetylacetone provided a quantity of 0.67 mmol g-1 of organic groups attached to the support and 0.63 mmol g-1 of copper. This material was used as a stationary phase in IMAC (immobilized metal affinity chromatography), to separate alpha-lactoalbumin from bovine milk whey. The results showed an efficient separation in the chromatographic column. The possibility of reutilization of the stationary phase was also investigated.
authors.affiliation: Departamento de Quimica, F.C. Unesp, Bauru, S.P., 17033-360, Brazil
authors.initials: A
authors.lastname: Gambero
authors.initials: LT
authors.lastname: Kubota
authors.initials: Y
authors.lastname: Gushikem
authors.initials: C
authors.lastname: Airoldi
authors.initials: JM
authors.lastname: Granjeiro
authors.initials: EM
authors.lastname: Taga
authors.initials: EFC
authors.lastname: Alcantara