title: Mapping and characterization of the origin of DNA replication of porcine circovirus.
abstract: The origin of DNA replication of porcine circovirus (PCV) was mapped to a 111-bp fragment. On top of a hairpin, a nonanucleotide (TAGTATTAC) homologous to nonanucleotides of other viruses was identified. Mutation of this element abolishes replication. PCV may be related to a virus family characterized by single-stranded circular DNA genomes, rolling-circle replication, and homology of their rep proteins.
authors.affiliation: Fachbereich Gentechnik und Genetik, Robert Koch-Institut, Berlin, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Mankertz
authors.forename: F
authors.initials: F
authors.lastname: Persson
authors.forename: J
authors.initials: J
authors.lastname: Mankertz
authors.forename: G
authors.initials: G
authors.lastname: Blaess
authors.forename: H J
authors.initials: HJ
authors.lastname: Buhk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D017925:Circovirus / Q000235:genetics*; D004279:DNA, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D018741:Replication Origin; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D004279:DNA, Viral