Acid anion profiles in dental plaque following consumption of cereal-based foods and fruits.
The aim of this study was to investigate the acid anions produced in plaque after chewing various cereal-based foods and fruits for one minute. Test foods were oranges, apples, bananas, Cornflakes, Branflakes, Weetabix, Alpen, white bread, wholemeal bread, rice and spaghetti, plus positive and negative controls of 10% sucrose and 10% sorbitol. 4 males and 3 females, aged 22-37 years, participated in the study .7 min from the start of chewing, 48-h plaque was collected from all accessible smooth surfaces, with no attempt to collect interproximal plaque, and centrifuged. Plaque fluid was withdrawn and analyzed by isotachophoresis for formate, succinate, lactate, acetate and propionate. At rest, acetate was the major anion present in plaque fluid, whereas following carbohydrate consumption, highest levels of lactate were detected followed by acetate. The amount of lactate only, detected in plaque fluid, was significantly correlated to the carbohydrate present in the food. It was concluded that important information regarding the acidogenicity of test foods is gained by studying the acid anion profile of plaque fluid.
Pollard
M A
MA
Child Dental Health, Leeds Dental Institute, England.
Higham
S M
SM
Curzon
M E
ME
Edgar
W M
WM
D000085:Acetates / Q000032:analysis; D000328:Adult; D000704:Analysis of Variance; D000838:Anions / Q000032:analysis*; D003773:Dental Plaque / Q000737:chemistry / Q000378:metabolism*; D004037:Diet, Cariogenic; D004040:Dietary Carbohydrates / Q000378:metabolism; D002523:Edible Grain; D005122:Exudates and Transudates / Q000737:chemistry; D005260:Female; D005638:Fruit; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000032:analysis; D008297:Male; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000378:metabolism
D000085:Acetates; D000838:Anions; D004040:Dietary Carbohydrates; D007773:Lactates