Quantification of peroxynitrite, superoxide, and peroxyl radicals by a new spin trap hydroxylamine 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine.
The reactions of hydroxylamine 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine hydrochloride (TEMPONE-H) with peroxynitrite, superoxide and peroxyl radicals were studied. It was shown that under these reactions TEMPONE-H is oxidized into a stable nitroxide 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidi-noxyl (TEMPONE). The reactivity of TEMPONE-H towards reactive oxygen species was compared with the spin traps DMPO and TMIO as well as with DMSO and SOD. The rate constants of reactions of TEMPONE-H with peroxynitrite and superoxide radicals were 6 x 10(9) M(-1)s(-1) and 1.2x10(4) M(-1)s(-1), respectively. Using TEMPONE-H the sensitivity in the detection of peroxynitrite or superoxide radical was about 10-fold higher than using the spin traps DMPO or TMIO. Thus, TEMPONE-H may be used as a spin trap in chemical and biological systems to quantify peroxynitrite and superoxide radical formation.
Dikalov
S
S
Institute of Chemical Kinetics & Combustion, Novosibirsk, Russia. Dikalov@kinetics.nsk.su
Skatchkov
M
M
Bassenge
E
E
D000578:Amidines; D004121:Dimethyl Sulfoxide; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005609:Free Radicals / Q000032:analysis; D007700:Kinetics; D008981:Molsidomine / Q000031:analogs & derivatives; D009566:Nitrates / Q000032:analysis*; D010545:Peroxides / Q000032:analysis*; D010880:Piperidines; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013481:Superoxides / Q000032:analysis*; D014216:Triacetoneamine-N-Oxyl
D000578:Amidines; D005609:Free Radicals; D009566:Nitrates; D010545:Peroxides; D010880:Piperidines; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013481:Superoxides; C039642:peroxynitric acid; D014216:Triacetoneamine-N-Oxyl; C049375:perhydroxyl radical; C103674:1-hydroxy-2,2,6,6-tetramethyl-4-oxopiperidine; C002385:linsidomine; C046728:2,2'-azobis(2-amidinopropane); D008981:Molsidomine; D004121:Dimethyl Sulfoxide