Identification of NAP-22 and GAP-43 (neuromodulin) as major protein components in a Triton insoluble low density fraction of rat brain.
NAP-22 is a membrane-localized brain enriched acidic protein having a Ca(2+)-dependent calmodulin binding activity. Further fractionation of the NAP-22 containing membrane showed the localization of NAP-22 in a Triton insoluble fraction of low density. Besides NAP-22, this fraction was found to contain GAP-43 (neuromodulin), trimeric G proteins, and some GPI-anchored proteins such as Thy-1 and N-CAM-120. Presence of some protein tyrosine kinases, such as src and fyn, was also shown.
Maekawa
S
S
Department of Biotechnology, Faculty of Textile Science, Kyoto Institute of Technology, Japan. smaekawa@ipc.kit.ac.jp
Kumanogoh
H
H
Funatsu
N
N
Takei
N
N
Inoue
K
K
Endo
Y
Y
Hamada
K
K
Sokawa
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002148:Calmodulin-Binding Proteins / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D003598:Cytoskeletal Proteins; D015180:Electrophoresis, Gel, Two-Dimensional; D019922:GAP-43 Protein; D019204:GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification; D008562:Membrane Glycoproteins / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D019006:Neural Cell Adhesion Molecules / Q000302:isolation & purification; D017830:Octoxynol; D051381:Rats; D012995:Solubility; D018800:Thy-1 Antigens / Q000302:isolation & purification
D002148:Calmodulin-Binding Proteins; D003598:Cytoskeletal Proteins; D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules; D018800:Thy-1 Antigens; C066853:Basp1 protein, rat; D017830:Octoxynol; D019204:GTP-Binding Proteins