title: Activation of human macrophage fungistatic activity against Histoplasma capsulatum upon adherence to type 1 collagen matrices.
abstract: Human monocyte/macrophages (Mphi) were adhered to extracellular matrix proteins, and the intracellular growth of Histoplasma capsulatum (Hc) yeasts were quantified and compared with their growth in Mphi adhered to plastic. Freshly isolated monocytes and cultured monocyte/derived Mphi adhered to type 1 collagen gels, but not to nongelled collagen-, fibronectin-, laminin-, or vitronectin-coated surfaces, demonstrated significant fungistatic activity against Hc yeasts. Activation of Mphi developed immediately upon adherence to the collagen matrices (1 h) and did not require additional time in culture. In addition, many of the yeasts were digested by 24 h postinfection. Mphi adhered to collagen maintained their fungistatic activity for up to 4 days, during which time monolayers cultured on plastic were destroyed. Culture of Mphi in the presence of IFN-gamma or TNF-alpha for 24 h before infection did not augment the fungistatic activity of collagen-adherent Mphi. Likewise, culture of monocytes on collagen gels with IL-3, granulocyte-Mphi CSF (GM-CSF) or Mphi CSF (M-CSF) for 7 days did not enhance Mphi fungistatic activity above that obtained by monocytes cultured on collagen alone. The mechanism(s) of Mphi-mediated fungistasis was not associated with production of toxic oxygen radicals, nitric oxide, or the restriction of intracellular iron. However, experiments with horseradish peroxidase-labeled gold colloids and immunoelectron microscopy demonstrated that phagolysosomal fusion, which is minimal in Hc-infected Mphi adhered to plastic, is enhanced significantly at both 1 h and 24 h postinfection in Mphi adhered to collagen matrices. These data suggest that in vivo, matrix-bound Mphi may express a previously unrecognized antifungal activity that proceeds in the absence of exogenous cytokines and is mediated, in part, by overcoming the capacity of Hc yeasts to inhibit Mphi phagolysosomal fusion.
authors.affiliation: Department of Medicine, University of Cincinnati College of Medicine, OH 45267, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Newman
authors.forename: L
authors.initials: L
authors.lastname: Gootee
authors.forename: C
authors.initials: C
authors.lastname: Kidd
authors.forename: G M
authors.initials: GM
authors.lastname: Ciraolo
authors.forename: R
authors.initials: R
authors.lastname: Morris
mesh_terms: D002448:Cell Adhesion / Q000276:immunology; D003094:Collagen / Q000502:physiology*; D016207:Cytokines / Q000494:pharmacology; D005782:Gels; D006658:Histoplasma / Q000254:growth & development* / Q000276:immunology; D006660:Histoplasmosis / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*; D006801:Humans; D007424:Intracellular Fluid / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D007501:Iron / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology* / Q000502:physiology; D010588:Phagosomes / Q000276:immunology / Q000382:microbiology
chemical_list: D016207:Cytokines; D005782:Gels; D003094:Collagen; D007501:Iron