PCR detection of sequences similar to the AS-48 structural gene in bacteriocin-producing enterococci.
Three oligonucleotide primers, specific for the structural gene of antimicrobial peptide AS-48, were used to study the distribution of sequences similar to this gene in 15 independently isolated bacteriocin-producing Enterococcus strains by semi-nested PCR. Eight of 10 Ent. faecalis strains and three of five Ent. faecium strains gave a positive result in both reactions of the semi-nested PCR. These findings indicate that bacteriocins produced by many enterococcal strains are very closely related or even identical to peptide AS-48.
Joosten
H M
HM
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
Rodriguez
E
E
Nuñez
M
M
D001430:Bacteriocins / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D013293:Enterococcus faecalis / Q000235:genetics*; D016984:Enterococcus faecium / Q000235:genetics*; D005798:Genes, Bacterial; D010455:Peptides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012689:Sequence Homology, Nucleic Acid
D001430:Bacteriocins; D017931:DNA Primers; D010455:Peptides