Identification of a salicylic acid-responsive element in the promoter of the tobacco pathogenesis-related beta-1,3-glucanase gene, PR-2d.
The tobacco pathogenesis-related PR-2d gene encodes an acidic beta-1,3-glucanase. Expression of the PR-2d: uidA(GUS) chimeric gene is induced in leaves undergoing the hypersensitive resistance response to tobacco mosaic virus and after treatment with salicylic acid (SA), a chemical believed to play an important role(s) in disease resistance. We have constructed transgenic tobacco plants which carry various segments of the PR-2d promoter fused to a heterologous core 35S promoter driving the uidA(GUS) reporter gene. Their analysis indicates that sequences from -364 to -288 upstream of the PR-2d transcription start site confer a high level of activation by SA (20-fold). Mutations within this sequence, located between -339 and -333, depressed SA activation. This region is also required for the SA-inducibility of a truncated PR-2d:GUS chimeric gene. Contained within this region is a 25 bp element located between -348 and -324 which was specifically recognized by nuclear factors from tobacco leaves. No conclusive differences were observed in the ability of proteins in nuclear extracts from water-treated versus SA-treated plants to bind to this cis element in vitro.
Shah
J
J
Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08855, USA.
Klessig
D F
DF
D001665:Binding Sites; D019175:DNA Methylation; D004252:DNA Mutational Analysis; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D043326:Glucan 1,3-beta-Glucosidase; D007113:Immunity, Innate; D009687:Nuclear Proteins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012459:Salicylates / Q000494:pharmacology*; D020156:Salicylic Acid; D017384:Sequence Deletion; D015398:Signal Transduction; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics; D001617:beta-Glucosidase / Q000235:genetics*
D009687:Nuclear Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012459:Salicylates; C053376:pathogenesis-related proteins, plant; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase; D020156:Salicylic Acid