Constitutive expression of human ribosomal protein L7 arrests the cell cycle in G1 and induces apoptosis in Jurkat T-lymphoma cells.
Protein L7 is involved in translational control in eucaryotic cells as indicated by its association with ribosomes, its capability to inhibit specifically the cell-free translation of distinct mRNAs, and its interference with the synthesis of two major nucleus-associated proteins in L7 cDNA-transfected Jurkat T-lymphoma cells [F. Neumann et al. (1995) Nucleic Acids Res. 23, 195]. In this report we show that the constitutive expression of protein L7 in Jurkat cells leads to an arrest in G1 of the cell cycle and induces apoptosis as a consequence of cell-to-cell contact. Treatment of the L7 transfectants with the inhibitor of translation cycloheximide, at doses which do not affect untransfected cells, enhances their sensitivity to the induction of apoptosis. These results suggest that L7 can interfere with the translation of proteins which control cell cycle progression and/or the initiation of the apoptotic pathways.
Neumann
F
F
Faculty of Biology, University of Konstanz, Germany. frank.neumann@uni-konstanz.de
Krawinkel
U
U
D017209:Apoptosis / Q000502:physiology*; D002453:Cell Cycle; D016193:G1 Phase; D015870:Gene Expression; D006801:Humans; D019169:Jurkat Cells; D012269:Ribosomal Proteins / Q000235:genetics*; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection
C053634:RPL7 protein, human; D012269:Ribosomal Proteins