Modulation of a competitive N-methyl-D-aspartate receptor antagonist binding by zinc oxide.
Zinc through a zinc binding site is known to modulate the binding of agonists at the NMDA receptor. In the present study, the ability of zinc oxide to alter the specific binding of [3H]CGP-39653, a competitive NMDA receptor antagonist, was determined in homogenate of rat brain tissue. Analysis of saturation experiments indicated that zinc oxide significantly increased the Kd without changing Bmax of [3H]CGP-39653 binding. Furthermore, the effect of ZnO on glutamate and glycine displacement of [3H]CGP-39653 binding was determined. The results of the [3H]CGP-39653 displacement study indicated that ZnO decreases the glutamate and glycine displacement of [3H]CGP-39653 binding.
Terse
P S
PS
College of Pharmacy, Xavier University of Louisiana, New Orleans 70125, USA.
Komiskey
H L
HL
D015763:2-Amino-5-phosphonovalerate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects*; D015034:Zinc Oxide / Q000494:pharmacology*
D018691:Excitatory Amino Acid Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; C069052:CGP 39653; D015763:2-Amino-5-phosphonovalerate; D015034:Zinc Oxide