title: Improved distribution of pulmonary flush solution to the tracheobronchial wall in pulmonary transplantation.
abstract: This report describes the experimental results obtained with conventional (pulmonary artery, PA) flushing versus retrograde perfusion (via left atrium, LA) using 99mTc-labeled macroaggregated albumin (MAA-99mTc) to ascertain the distribution throughout the tracheobronchial (TB) tree in 10 Large-White pigs. Lung preservation was achieved with 4 degrees C Euro-Collins solution (60 ml/kg) instilled via PA (n = 5) or LA (n = 5). Simultaneously, MAA-99mTc was given using the same respective route and the isotope uptake quantified at different TB levels after heart-lung block harvest and dissection of all tissue adjacent to TB: proximal and distal trachea and right and left main bronchi. Retrograde distribution resulted in a significantly higher 99mTc count compared to the PA route (p < 0.01).
authors.affiliation: Thoracic and Cardiovascular Surgery, Hospital Pureta de Hierro, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Varela
authors.forename: C G
authors.initials: CG
authors.lastname: Montero
authors.forename: M
authors.initials: M
authors.lastname: Córdoba
authors.forename: A
authors.initials: A
authors.lastname: Antequera
authors.forename: M
authors.initials: M
authors.lastname: Pérez
authors.forename: M J
authors.initials: MJ
authors.lastname: Tabuenca
authors.forename: J
authors.initials: J
authors.lastname: Ortiz Berrocal
authors.forename: F J
authors.initials: FJ
authors.lastname: Tendillo
authors.forename: A
authors.initials: A
authors.lastname: Mascias
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.forename: J L
authors.initials: JL
authors.lastname: Castillo Olivares
mesh_terms: D000818:Animals; D001980:Bronchi / Q000000981:diagnostic imaging / Q000378:metabolism*; D006325:Heart Atria; D006982:Hypertonic Solutions / Q000378:metabolism*; D007261:Infusions, Intra-Arterial; D008168:Lung / Q000502:physiology*; D016040:Lung Transplantation; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000378:metabolism*; D010477:Perfusion / Q000379:methods; D011651:Pulmonary Artery; D011877:Radionuclide Imaging; D013552:Swine; D013668:Technetium Tc 99m Aggregated Albumin; D014132:Trachea / Q000000981:diagnostic imaging / Q000378:metabolism*
chemical_list: C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D019188:Organ Preservation Solutions; D013668:Technetium Tc 99m Aggregated Albumin