Spontaneous establishment of an Epstein-Barr virus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient.
An Epstein-Barr virus (EBV)-infected fibroblast line, designated DSEK, was spontaneously established from synovial tissue of a patient with rheumatoid arthritis (RA). DSEK cells expressed EBV nuclear antigens EBNA-1 and EBNA-2 and latent membrane protein LMP-1. Cell surface markers of DSEK cells were similar to those of EBV-negative fibroblast clones derived from synoviocytes and were negative for lymphocyte and macrophage markers. DSEK cells expressed CD44, CD58, and HLA-DR antigens and spontaneously produced interleukin-10 basic fibroblast growth factor and transforming growth factor beta1. These results indicate that rheumatoid synoviocytes can be a target for EBV infection and suggest that EBV may play a role in the pathogenesis of RA.
Koide
J
J
Department of Internal Medicine, Saitama Medical Center, Kawagoe, Japan.
Takada
K
K
Sugiura
M
M
Sekine
H
H
Ito
T
T
Saito
K
K
Mori
S
S
Takeuchi
T
T
Uchida
S
S
Abe
T
T
D001172:Arthritis, Rheumatoid / Q000473:pathology / Q000821:virology*; D015415:Biomarkers; D002455:Cell Division; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000821:virology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D013582:Synovial Fluid
D015415:Biomarkers; D016207:Cytokines