title: Structural polypeptides of different clinical strains of avian adenovirus type-1.
abstract: The polypeptides of two liver strains, two ovarian strains and one standard strain of avian adenovirus-1 (AAV-1) were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot using polyclonal antibodies to all the strains and isoelectric focusing (IEF). All the strains had 11 polypeptides and molecular weight varied between 9 and 100 kDa. The polypeptide pattern was similar in all the strains as revealed by SDS-PAGE and Western blot. However, some differences among the strains on the basis of isoelectric point of polypeptide were observed by IEF.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, Bidhan Chandra Krishi Viswavidylaya, Mohanpur, Nadia, India.
authors.forename: N K
authors.initials: NK
authors.lastname: Maiti
authors.forename: P
authors.initials: P
authors.lastname: Sarkar
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D000259:Aviadenovirus / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008854:Microscopy, Electron; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins