title: Generation of multinuclear tartrate-resistant acid phosphatase positive osteoclasts in liquid culture of purified human peripheral blood CD34+ progenitors.
abstract: Circulating CD34+ cells were isolated from leukapheresis products collected from patients with ovarian cancer. CD34 contaminating cells, identified immediately after immunoselection, ranged from 5% to 25% in five different experiments and were predominantly CD3+ T-lymphocytes (range 2-12%), CD3+/CD16+/CD56+ natural killer cells (range 2-11%) and rare mature CD15+/ CD11b+ granulocytes (range 1-2%). CD34+ cells were cultured in liquid medium in the presence of interleukin-3, granulocyte-macrophage colony stimulating factor. stem cell factor, granulocyte colony stimulating factor and a powerful proliferation with prevalent differentiation along the granulocytic/monocytic lineage was obtained. After 10 d of culture a small but consistent number of early multinucleated osteoclasts were identified with a frequency of one cell per 700 granulocytic/monocytic cells, as revealed by cytologic examination. This observation was confirmed by staining for tartrate-resistant acid phosphatase activity which revealed red multinucleated elements with a frequency comparable to that reported above. Conversely, no osteoclasts were observed in those cultures in which macrophage overgrowth was obtained by culturing CD34+ cells until day 35. These observations suggest that circulating progenitors have a multilineage potential in vitro and contribute to the clarification of osteoclast development in humans: additionally, they provide the basis for the future development of optimized osteoclast culture techniques in liquid medium and the basic culture system, to test the distinct activity of 1,25(OH)2D3. parathyroid hormone interleukin-11 and of other cytokines on osteoclast development in humans.
authors.affiliation: Centro Ricerche per la Manipolazione dei Costituenti Ematici, Catholic University, Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Pierelli
authors.forename: G
authors.initials: G
authors.lastname: Scambia
authors.forename: G
authors.initials: G
authors.lastname: d'Onofrio
authors.forename: M
authors.initials: M
authors.lastname: Ciarli
authors.forename: A
authors.initials: A
authors.lastname: Fattorossi
authors.forename: G
authors.initials: G
authors.lastname: Bonanno
authors.forename: G
authors.initials: G
authors.lastname: Menichella
authors.forename: A
authors.initials: A
authors.lastname: Battaglia
authors.forename: P
authors.initials: P
authors.lastname: Benedetti Panici
authors.forename: M
authors.initials: M
authors.lastname: Tommasi
authors.forename: S
authors.initials: S
authors.lastname: Mancuso
authors.forename: G
authors.initials: G
authors.lastname: Leone
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D018952:Antigens, CD34; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism*; D010010:Osteoclasts / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D019089:Stem Cell Factor / Q000494:pharmacology; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D018952:Antigens, CD34; D007377:Interleukin-3; D007527:Isoenzymes; D019089:Stem Cell Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase