title: Role for the amino-terminal region of human TBP in U6 snRNA transcription.
abstract: Basal transcription from the human RNA polymerase III U6 promoter depends on a TATA box that recruits the TATA box-binding protein (TBP) and a proximal sequence element that recruits the small nuclear RNA (snRNA)-activating protein complex (SNAPc). TBP consists of a conserved carboxyl-terminal domain that performs all known functions of the protein and a nonconserved amino-terminal region of unknown function. Here, the amino-terminal region is shown to down-regulate binding of TBP to the U6 TATA box, mediate cooperative binding with SNAPc to the U6 promoter, and enhance U6 transcription.
authors.affiliation: Howard Hughes Medical Institute and Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
authors.forename: V
authors.initials: V
authors.lastname: Mittal
authors.forename: N
authors.initials: N
authors.lastname: Hernandez
mesh_terms: D000595:Amino Acid Sequence; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016385:TATA Box; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D011506:Proteins; D012342:RNA, Small Nuclear; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C093069:SNAPC1 protein, human; C099437:SNAPC2 protein, human; C101746:SNAPC3 protein, human; D035181:TATA-Box Binding Protein; D014157:Transcription Factors