title: Moderate hyperthyroidism reduces liver amino nitrogen conversion, muscle nitrogen contents and overall nitrogen balance in rats.
abstract: There are conflicting data on the effect of thyroid hormones on nitrogen metabolism. We determined the basal blood amino nitrogen (amino-N) concentrations, the urea nitrogen (urea-N) synthesis rate and the maximum hepatic capacity of urea nitrogen synthesis during saturating infusion of alanine, in moderately acutely (24 h) and chronically (7 days) hyperthyroid rats and compared this with changes in organ nitrogen contents in muscles and kidney, nitrogen excretion and nitrogen balance. Forty-three rats were made acutely hyperthyroid through administration of 5 microg 100 g(-1) triiodothyronine twice daily (T3: 2.2 +/- 0.7 vs. 0.87 +/- 0.04 nmol L(-1), P < 0.01). Fifty-one rats were made chronically hyperthyroid through administration of 12.5 microg 100 g(-1) thyroxine twice daily (T3: 2.63 +/- 0.18 vs. 0.87 +/- 0.04 nmol L(-1), P < 0.01). Weight gain was halved in this group. Both acute and chronic hyperthyroidism increased basal blood amino-N concentration in both groups by 16% (4.5 +/- 0.15 vs. 3.9 +/- 0.13 mmol L(-1) and 4.7 +/- 0.12 vs. 3.9 +/- 0.13 mmol L(-1), respectively, P < 0.01), and decreased basal urea-N synthesis rate in both groups by 30% [2.7 +/- 0.3 vs. 4.1 +/- 0.3 micromol (min x 100 g)(-1) and 3.1 +/- 0.3 vs. 4.1 +/- 0.3 micromol (min x 100g)(-1), respectively, P < 0.01]. The capacity of urea-N synthesis during saturation fell in both groups by 35% compared with controls [6.5 +/- 0.4 vs. 9.3 +/- 0.5 micromol (min x 100 g)(-1) and 5.7 +/- 0.5 vs. 9.3 +/- 0.6 micromol (min x 100g)(-1), respectively, P < 0.01]. Nitrogen contents in the muscles, soleus and extensor digitorum longus, of chronically hyperthyroid rats decreased by 22% and 11%, respectively, whereas kidney N-content increased by 12% (P < 0.05). N-balance and urinary urea-N excretion fell by 30%, whereas faeces-N excretion increased by 80% in hyperthyroid rats. Overall liver function assessed by galactose elimination capacity did not differ among groups. Both acute and chronic moderate hyperthyroidism increase blood amino-N and decrease basal and maximum rate of urea formation. Furthermore, chronic hyperthyroidism reduces N-contents of muscles, urinary urea-N excretion and N-balance. Thyroid hormones thus mobilize muscle-N, whereas amino-N in the liver is spared from irretrievable conversion into urea.
authors.affiliation: Department of Medicine V, Aarhus University Hospital, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Grofte
authors.forename: T
authors.initials: T
authors.lastname: Wolthers
authors.forename: N
authors.initials: N
authors.lastname: Moller
authors.forename: J O
authors.initials: JO
authors.lastname: Jorgensen
authors.forename: A
authors.initials: A
authors.lastname: Flyvbjerg
authors.forename: H
authors.initials: H
authors.lastname: Orskov
authors.forename: H
authors.initials: H
authors.lastname: Vilstrup
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D001806:Blood Urea Nitrogen; D001835:Body Weight; D004327:Drinking Behavior; D005247:Feeding Behavior; D005260:Female; D005690:Galactose / Q000652:urine; D006339:Heart Rate; D006980:Hyperthyroidism / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D007334:Insulin-Like Growth Factor I / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood; D014508:Urea / Q000032:analysis / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D014284:Triiodothyronine; D007334:Insulin-Like Growth Factor I; D014508:Urea; D013974:Thyroxine; D005690:Galactose