In vivo EPR: an effective new tool for studying pathophysiology, physiology and pharmacology.
The development of spectrometers working at lower frequencies with improved resonators now permits the routine use of non-invasive EPR spectroscopy in vivo. The capabilities of EPR spectra to reflect environmental conditions, combined with the use of paramagnetic materials as selective non-toxic labels, has led to increasingly widespread and productive applications of the technique to complex problems involving physiology, pharmacology and pathophysiology. Some of the especially promising applications in which EPR techniques uniquely appear to provide valuable information are illustrated, including the measurement of oxygen and oxygen gradients, monitoring of the metabolism of xenobiotics, monitoring pharmacokinetics of drugs, measurement of perfusion, measurement of pH, recognition and labeling of receptors, and characterization of drug releasing systems.
Mäder
K
K
Department of Radiology, Dartmouth Medical School, Hanover, NH 03755, USA.
Gallez
B
B
Swartz
H M
HM
D000818:Animals; D016503:Drug Delivery Systems; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals / Q000032:analysis / Q000528:radiation effects; D006801:Humans; D009589:Nitrogen Oxides / Q000032:analysis / Q000528:radiation effects; D010100:Oxygen / Q000032:analysis; D010600:Pharmacology / Q000379:methods*; D010827:Physiology / Q000379:methods*; D013113:Spin Labels; D015262:Xenobiotics / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects
D005609:Free Radicals; D009589:Nitrogen Oxides; D013113:Spin Labels; D015262:Xenobiotics; C039900:nitroxyl; D010100:Oxygen