Preparation and characterization of immunostimulating complexes (ISCOMs) of Japanese encephalitis virus.
ISCOMs (immunostimulating complexes) were prepared from envelope glycoprotein (Egp) of Japanese encephalitis (JE) virus. ISCOMs showed a single band of the viral Egp in SDS-PAGE, which reacted with polyclonal and monoclonal antibody (MAb) raised against Egp. Comparison between the epitopes exposed on JE virion and JE ISCOMs, by antigen capture ELISA, utilizing a panel of domain-specific MAbs, revealed identical epitopes exposed on the Egp incorporated in ISCOMs and the whole virion. Electron micrographs of ISCOMs showed spherical cage-like structures of 35 nm. ISCOMs with Egp were good immunogenes, which stimulated high titres of neutralizing antibodies, both in mice and rabbits.
Yeolekar
L R
LR
National Institute of Virology, Maharashtra, India.
Banerjee
K
K
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay; D016882:ISCOMs / Q000096:biosynthesis* / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D062605:Quillaja Saponins; D012367:RNA, Viral / Q000032:analysis; D011817:Rabbits; D012503:Saponins
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D016882:ISCOMs; D062605:Quillaja Saponins; D012367:RNA, Viral; D012503:Saponins; C046386:Quil A