DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter.
The specific interaction of the upstream element-containing promoter of the Escherichia coli acetate operon with either the RNA polymerase holoenzyme or its alpha subunit has been analyzed by the base removal method. Our results indicate that: (i) direct and specific base contacts can be detected in the acetate promoter-alpha subunit complex; (ii) base elimination in the upstream element of the acetate promoter enhances the binding of RNA polymerase. A similar effect is observed when studying the interactions between RNA polymerase and the rrnB ribosomal operon P1 promoter.
Nègre
D
D
Institut de Biologie et Chimie des Proteines, Centre National de la Recherche Scientifique, Lyon, France.
Bonod-Bidaud
C
C
Oudot
C
C
Prost
J F
JF
Kolb
A
A
Ishihama
A
A
Cozzone
A J
AJ
Cortay
J C
JC
D000085:Acetates / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004269:DNA, Bacterial / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D012407:rRNA Operon
D000085:Acetates; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases