Inducible expression of a Porphyromonas gingivalis W83 membrane-associated protease.
The Tpr protease of Porphyromonas gingivalis W83 is a membrane-associated enzyme capable of hydrolyzing a chromogenic bacterial collagenase substrate. An isogenic mutant lacking a functional tpr gene had a greatly reduced ability to hydrolyze the collagenase substrate. Activity was restored to the tpr mutant by introducing a shuttle plasmid containing the tpr gene. Expression of the gene is induced by nutrient limitation, as shown by enzymatic and Northern analyses.
Park
Y
Y
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
Lu
B
B
Mazur
C
C
McBride
B C
BC
D017364:Collagenases / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000235:genetics*; D008666:Metalloendopeptidases / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000201:enzymology*
D008565:Membrane Proteins; D010450:Endopeptidases; D017364:Collagenases; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase