title: Chiral separation of chloroquine using heparin as a chiral selector in high-performance liquid chromatography.
abstract: The chiral selectivity of a commercially available heparin affinity column was investigated for chloroquine. The (+) enantiomer eluted first, which is consistent with results reported previously using heparin as a chiral additive in capillary zone electrophoresis. The effects of pH, ionic strength, and organic modifier on the enantiodiscrimination were explored. A combination of electrostatic and hydrophobic interactions seems to play an important role in the enantiodiscrimination exhibited by this novel phase.
authors.affiliation: Department of Chemistry, University of Hawaii-Manoa, Honolulu 96822, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Staicup
authors.forename: K H
authors.initials: KH
authors.lastname: Gahm
authors.forename: M
authors.initials: M
authors.lastname: Baldueza
mesh_terms: D002738:Chloroquine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006493:Heparin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002738:Chloroquine; D006493:Heparin