title: Evaluation of HBsAg carrier rate in acute viral hepatitis and high risk individuals using RPHA and ELISA.
abstract: A total of 600 individuals including 500 cases of hepatitis and 100 individuals at high risk for developing hepatitis were screened for hepatitis B surface antigen (HBsAg) using reverse passive haemagglutination (RPHA) and enzyme linked immunosorbent assay (ELISA). HBsAg carrier rate in clinically diagnosed cases of hepatitis was 38% and 32.4% by RPHA and ELISA respectively. In high risk individuals, the carrier rate was 14% by RPHA and 11% by ELISA. Taking ELISA as gold standard, RPHA showed 5.33% false positivity and 0.33% false negativity. The over all correlation between RPHA and ELISA was to the tune of 82.66%.
authors.affiliation: Department of Microbiology, Goa Medical College, Bamboli.
authors.forename: A
authors.initials: A
authors.lastname: Korgaonkar
authors.forename: M P
authors.initials: MP
authors.lastname: Verenkar
authors.forename: R
authors.initials: R
authors.lastname: Savio
authors.forename: M J
authors.initials: MJ
authors.lastname: Pinto
authors.forename: I
authors.initials: I
authors.lastname: Singh
mesh_terms: D000208:Acute Disease; D002353:Carrier State / Q000453:epidemiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006386:Hemagglutination Tests / Q000379:methods; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006525:Hepatitis, Viral, Human / Q000453:epidemiology*; D006801:Humans
chemical_list: D006514:Hepatitis B Surface Antigens