Purification, characterization and immobilization of proteinase inhibitors from Stichodactyla helianthus.
Isolation of proteinase inhibitors from the sea anemone Stichodactyla helianthus was achieved by trichloroacetic acid treatment of the aqueous extract followed by affinity chromatography on trypsin-Sepharose and ion-exchange chromatography on CM-cellulose. The average molecular mass of the major inhibitor (ShPI-I) obtained by fast atom bombardment mass spectrometry (FAB-MS) was 6110.6 Da. The amino acid sequence was determined by FAB-MS combined with manual Edman degradation, digestions with endopeptidases and exopeptidases and automatic sequencing. The sequence of ShPI-I (55 amino acids) was compared with those reported in the SwissProt database for several proteinase inhibitors and significant similarity to inhibitors belonging to the Kunitz family was observed. ShPI-I exhibits a broad specificity for serine, cysteine and aspartic proteinases. The dissociation constants of the complexes formed with different enzymes were determined. The affinity-purified fraction (PI) was immobilized on Sepharose and used in the purification of different classes of proteinases.
Delfín
J
J
Facultad de Biologia, Universidad de la Habana, Cuba.
Martínez
I
I
Antuch
W
W
Morera
V
V
González
Y
Y
Rodríguez
R
R
Márquez
M
M
Saroyán
A
A
Larionova
N
N
Díaz
J
J
Padrón
G
G
Chávez
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002847:Chromatography, Agarose; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D012615:Sea Anemones / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D004800:Enzymes, Immobilized; D011480:Protease Inhibitors; C080476:ShPI proteinase inhibitor; D014360:Trypsin Inhibitor, Kunitz Soybean