Use of monoclonal antibodies for immunohistochemical study of bovine spleen on frozen sections.
Many monoclonal antibodies reactive with bovine leukocyte differentiation antigens are now available. Immunohistochemical staining on frozen sections using these monoclonal antibodies permits study of the functional morphology of bovine spleen. This study confirms accepted notions (B and T dependent-zones) and supplies complementary data about the repartition of CD4 and CD8 cells, gamma delta T cells, MHC (Major Histocompatibility Complex) II expression, and macrophages.
Delverdier
M
M
Laboratoire associé INRA, Physiopathologie des Maladies Respiratoires des Ruminants, Ecole Nationale Vétérinaire, Toulouse, France.
Abella-Bourges
N
N
Schelcher
F
F
Raymond
I
I
Espinasse
J
J
Cabanie
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D015704:CD4 Antigens / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D016827:CD8 Antigens / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D002417:Cattle / Q000033:anatomy & histology / Q000378:metabolism*; D005260:Female; D005629:Frozen Sections / Q000662:veterinary; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007962:Leukocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D008264:Macrophages / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D013154:Spleen / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens; D000949:Histocompatibility Antigens Class II