Characteristics of tumor cell bioactivity in oncogenic osteomalacia.
Oncogenic osteomalacia is a condition where renal phosphate wasting occurs causing defective mineralisation, in the presence of a tumor. Cultures of cells were established from a hemangiopericytoma resected from a patient with oncogenic osteomalacia. Conditioned media from the cells inhibited phosphate uptake in opossum kidney cells and stimulated of cAMP in rat osteosarcoma cells, a standard parathyroid hormone (PTH)-like assay. This cAMP stimulation was suppressed by the PTH analogue, 3-34 bPTH and also by heat and trypsin treatment of the media. Tests of conditioned media for PTH and parathyroid hormone related protein (PTHrP) immunoreactivity were negative, however, and no hybridisation to probes for PTH, PTHrP or human stanniocalcin was detected in tumor cell RNA on Northern blot. These data support the hypothesis that tumors responsible for oncogenic osteomalacia produce a humoral substance that reduces renal phosphate reabsorption and provide evidence that the factor may act via PTH/PTHrP receptors.
Nelson
A E
AE
Department of Molecular Genetics, Kolling Institute of Medical Research, Royal North Shore Hospital, Sydney, NSW, Australia.
Namkung
H J
HJ
Patava
J
J
Wilkinson
M R
MR
Chang
A C
AC
Reddel
R R
RR
Robinson
B G
BG
Mason
R S
RS
D000818:Animals; D001692:Biological Transport; D002999:Clone Cells; D017077:Culture Media, Conditioned; D000242:Cyclic AMP / Q000096:biosynthesis; D006393:Hemangiopericytoma / Q000150:complications / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D017674:Hypophosphatemia; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D009893:Opossums; D010018:Osteomalacia / Q000209:etiology* / Q000473:pathology; D010281:Parathyroid Hormone / Q000494:pharmacology; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D018016:Receptors, Parathyroid Hormone / Q000502:physiology; D014357:Trypsin; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D009363:Neoplasm Proteins; C470121:PTHLH protein, human; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D010710:Phosphates; D011506:Proteins; D012333:RNA, Messenger; D018016:Receptors, Parathyroid Hormone; C031617:parathyroid hormone (3-34); D000242:Cyclic AMP; D014357:Trypsin