Rapid method for the analysis of itraconazole and hydroxyitraconazole in serum by high-performance liquid chromatography.
An assay for the determination of itraconazole and its active metabolite hydroxyitraconazole in serum has been developed, using ketoconazole as internal standard. The procedure involved a one-step liquid-liquid extraction of the drug, its metabolite and the internal standard, followed by their separation by reversed-phase HPLC. In this paper the validation of this method is described.
Compas
D
D
Laboratory of the Pharmacy Department, University Hospital Vrije Universiteit, Amsterdam, Netherlands.
Touw
D J
DJ
de Goede
P N
PN
D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D017964:Itraconazole / Q000031:analogs & derivatives* / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; C084794:hydroxyitraconazole; D017964:Itraconazole