title: A simple and sensitive quantitation of N,N-dimethyltryptamine by gas chromatography with surface ionization detection.
abstract: A simple and sensitive method for determination of N,N-dimethyltryptamine (DMT) by gas chromatography (GC) with surface ionization detection (SID) is presented. Whole blood or urine, containing DMT and gramine (internal standard), was subjected to solid-phase extraction with a Sep-Pak C18 cartridge before analysis by GC-SID. The calibration curve was linear in the DMT range of 1.25-20 ng/mL blood or urine. The detection limit of DMT was about 0.5 ng/mL (10 pg on-column). The recovery of both DMT and gramine spiked in biological fluids was above 86%.
authors.affiliation: Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ishii
authors.forename: H
authors.initials: H
authors.lastname: Seno
authors.forename: O
authors.initials: O
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Hattori
authors.forename: T
authors.initials: T
authors.lastname: Kumazawa
mesh_terms: D002138:Calibration; D002849:Chromatography, Gas / Q000295:instrumentation*; D006801:Humans; D004130:N,N-Dimethyltryptamine / Q000032:analysis* / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D004130:N,N-Dimethyltryptamine