title: The interorganellar interaction between distinct human mitochondria with deletion mutant mtDNA from a patient with mitochondrial disease and with HeLa mtDNA.
abstract: For the examination of possible intermitochondrial interaction of human mitochondria from different cells, cybrids were constructed by introducing HeLa mitochondria into cells with respiration-deficient (rho-) mitochondria. Respiration deficiency was due to the predominance of mutant mtDNA with a 5,196-base pair deletion including five tRNA genes (DeltamtDNA5196). The HeLa mtDNA and DeltamtDNA5196 encoded chloramphenicol-resistant (CAPr) and chloramphenicol-sensitive (CAPs) 16 S rRNA, respectively. The first evidence for the interaction was that polypeptides exclusively encoded by DeltamtDNA5196 were translated on the introduction of HeLa mitochondria, suggesting supplementation of the missing tRNAs by rho- mitochondria from HeLa mitochondria. Second, the exchange of mitochondrial rRNAs was observed; even in the presence of CAP, CAPs DeltamtDNA5196-specific polypeptides as well as those encoded by CAPr HeLa mtDNA were translated in the cybrids. These phenomena can be explained assuming that the translation in rho- mitochondria was restored by tRNAs and CAPr 16 S rRNA supplied from HeLa mitochondria, unambiguously indicating interorganellar interaction. These observations introduce a new concept of the dynamics of the mitochondrial genetic system and help in understanding the relationship among mtDNA mutations and expression of human mitochondrial diseases and aging.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Ibaraki 305, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Takai
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: Y i
authors.initials: Yi
authors.lastname: Goto
authors.forename: I
authors.initials: I
authors.lastname: Nonaka
authors.forename: J I
authors.initials: JI
authors.lastname: Hayashi
mesh_terms: D002702:Chloramphenicol Resistance; D002999:Clone Cells / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000737:chemistry*; D003576:Electron Transport Complex IV / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008854:Microscopy, Electron; D008928:Mitochondria / Q000737:chemistry*; D014176:Protein Biosynthesis / Q000187:drug effects; D012336:RNA, Ribosomal, 16S / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S; D003576:Electron Transport Complex IV