Serological analysis of monoclonal anti-E and -e with Japanese E/e variant red cells.
The agglutination patterns have been analyzed for the reaction between 24 monoclonal antibodies (MAB) with specificity for the Rh antigen E or e and red cells of E/e variant from Japanese blood donors. The MABs were tested for direct agglutination of papain treated cells at 20 degrees C. The reactions of a full titration series of each MAB with a E/e variant cell were compared to the reactions of that MAB with normal E + e + cells. Sixteen anti-E were divided into a minimum of 6 different agglutination patterns with 8 examples of E variant cells. Eight anti-e gave a minimum of 5 different agglutination patterns with 6 examples of e variant cells.
Uchikawa
M
M
Blood Group Section, Japanese Red Cross Central Blood Centre, Tokyo, Japan.
Toyoda
C
C
Shinozaki
K
K
Suzuki
Y
Y
Tsuneyama
H
H
Juji
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D012699:Serology
D000911:Antibodies, Monoclonal; D012204:Rh-Hr Blood-Group System