[The neurochemical, molecular and ultrastructural mechanisms of the formation of latent postresuscitation encephalopathy].
The content of total RNA and DNA, activity of Ca2+, Mg(2+)-dependent DNA endonuclease, and ultrastructural changes in nerve tissue cells were examined in the brain cortex of narcotized dogs 1 to 3 months after a 4-hour hemorrhagic shock (arterial pressure 40 mm Hg). A new variant of reconstruction of cell membranes and organelles formed by them was revealed, developing in the brain neurons in the course of adaptation during the first-third months of the postshock period. Evidently, the molecular base of development of an atypical variant of cell structure rearrangement in the remote period after shock is the internucleosomal fragmentation of a part of the DNA of nerve cells resultant from DNA endonucleolysis and subsequent information disintegration of a cell as a system. This distorts the process of biosynthesis of supramolecular ensembles, specifically, of nerve cell biomembranes.
Kozhura
V L
VL
Solov'eva
Zh V
ZhV
Novoderzhkina
I S
IS
Nosova
N V
NV
D000818:Animals; D001706:Biopsy; D001923:Brain Chemistry; D001927:Brain Diseases / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism / Q000648:ultrastructure; D004285:Dogs; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D012151:Resuscitation / Q000009:adverse effects*; D012771:Shock, Hemorrhagic / Q000150:complications / Q000378:metabolism / Q000473:pathology; D013997:Time Factors