Total and birch pollen-specific human IgE in mice with severe combined immunodeficiency transplanted with human peripheral blood lymphocytes: donor dependence, seasonal variation and in vivo half-life.
BACKGROUND
There is a need for animal in vivo models in the study of human allergy. The aim of the present experiments was to study production and catabolism of human IgE in mice with severe combined immunodeficiency transplanted with human peripheral blood lymphocytes (hu-PBL-SCID mice).


METHODS
Groups of SCID mice were transplanted intraperitoneally with hu-PBL from the same three donors in five experiments. Subgroups of transplanted mice were immunized with birch pollen. Production of human total and birch pollen-specific IgE in the hu-PBL-SCID mice was analyzed over a 7-week period.


RESULTS
Human IgE was detected in 93% of the hu-PBL-SCID mice, and the production showed reproducible donor-dependent kinetics. Production of birch pollen-specific human IgE, however, was seen only in mice transplanted with cells from birch pollen-allergic donors. A greater proportion of the mice produced specific IgE when the experiment was started in, or some months after a birch pollen season with high pollen counts. The half-lives of passively transferred human IgE were determined to be 24.0 and 23.4 h for total and birch pollen-specific IgE, respectively.


CONCLUSIONS
This study demonstrates that human IgE production in hu-PBL-SCID mice is very reproducible when the same donor is used several times. Specific IgE production in recipient mice seems to require the use of cell donors with the actual specific allergy, and is most readily obtained during or after a period of donor allergen exposure. The short half-lives found indicate that hu-PBL-SCID mice have a high ongoing production of human IgE.
Steinsvik
T E
TE
Department of Environmental Medicine, National Institute of Public Health, Oslo, Norway.
Aaberge
I S
IS
Gaarder
P I
PI
Bjønness
U
U
Løvik
M
M
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D005434:Flow Cytometry; D006207:Half-Life; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood; D017493:Leukocyte Common Antigens / Q000378:metabolism; D017710:Lymphocyte Transfusion; D008297:Male; D051379:Mice; D016513:Mice, SCID; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D016511:Severe Combined Immunodeficiency / Q000276:immunology*; D014183:Transplantation, Heterologous
D000485:Allergens; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D017493:Leukocyte Common Antigens