title: Determination of a new antiulcer agent, YJA-20379-2, in human plasma and urine by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic method was developed for the determination of a new antiulcer agent, YJA-20379-2, in human plasma and urine. The sample preparation was simple: 2.5-volume of acetonitrile was added to the biological sample to deproteinize. A 50-microliter aliquot of the supernatant was injected onto a C18 reversed-phase column. The mobile phase employed was methanol-0.1M Sørensen phosphate buffer of pH 7.0-H2O (75:2:25, v/v/v), and was run at a flow-rate of 1.0 ml/min. The column effluent was monitored by ultraviolet detector at 295 nm. The retention time for YJA-20379-2 was approximately 7.0 min. The detection limits for YJA-20379-2 in human plasma and urine were both 100 ng/ml. The coefficients of variation of the assay (within-day and between-day) were generally low (below 9.16%) for both the human plasma and urine. No interference from endogenous substances was found.
authors.affiliation: College of Pharmacy, Seoul National University, Shinlim-Dong, Kwanak-Gu, Korea.
authors.forename: W H
authors.initials: WH
authors.lastname: Yoon
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: S Y
authors.initials: SY
authors.lastname: Chung
authors.forename: M S
authors.initials: MS
authors.lastname: Chang
authors.forename: W S
authors.initials: WS
authors.lastname: Choi
authors.forename: S K
authors.initials: SK
authors.lastname: Sohn
authors.forename: M G
authors.initials: MG
authors.lastname: Lee
mesh_terms: D000897:Anti-Ulcer Agents / Q000097:blood* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007093:Imidazoles / Q000097:blood* / Q000652:urine*
chemical_list: D000897:Anti-Ulcer Agents; D007093:Imidazoles; C105753:YJA 20379-2