title: Murine epidermal lipoxygenase (Aloxe) encodes a 12-lipoxygenase isoform.
abstract: Using a combination of conventional screening procedures and polymerase chain reaction cloning, we have isolated a cDNA encoding an epidermis-type 12-lipoxygenase (e12-lipoxygenase) from mouse epidermis. The open reading frame corresponds to a protein of 662 amino acids and was found to be 99.8% identical to the ORF of an epidermal lipoxygenase gene Aloxe, described recently [Van Dijk et al. (1995) Biochim. Biophys. Acta 1259, 4-8]. When expressed in human embryonic kidney cells the recombinant protein could be shown to synthesize 12(S)-HETE from arachidonic acid. By fluorescence in situ hybridization the e12-lipoxygenase gene was localized to chromosome band 11 B1-B3.
authors.affiliation: Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Kinzig
authors.forename: G
authors.initials: G
authors.lastname: Fürstenberger
authors.forename: F
authors.initials: F
authors.lastname: Bürger
authors.forename: S
authors.initials: S
authors.lastname: Vogel
authors.forename: K
authors.initials: K
authors.lastname: Müller-Decker
authors.forename: A
authors.initials: A
authors.lastname: Mincheva
authors.forename: P
authors.initials: P
authors.lastname: Lichter
authors.forename: F
authors.initials: F
authors.lastname: Marks
authors.forename: P
authors.initials: P
authors.lastname: Krieg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000096:biosynthesis* / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D007668:Kidney; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012867:Skin / Q000201:enzymology*; D014162:Transfection
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007527:Isoenzymes; D001092:Arachidonate 12-Lipoxygenase