title: 19F-NMR studies of retinol transfer between cellular retinol binding proteins and phospholipid vesicles.
abstract: The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol and intracellular trafficking of retinol between sites of metabolic processing. 19F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Differences in how readily protein-bound retinol is released to lipid bilayers may lead to differences in how these two proteins modulate intracellular retinol metabolism.
authors.affiliation: Department of Medicine, Washington University, School of Medicine, St. Louis, MO 63110, USA.
authors.forename: D
authors.initials: D
authors.lastname: Rong
authors.forename: C L
authors.initials: CL
authors.lastname: Lin
authors.forename: D A
authors.initials: DA
authors.lastname: d'Avignon
authors.forename: A J
authors.initials: AJ
authors.lastname: Lovey
authors.forename: M
authors.initials: M
authors.lastname: Rosenberger
authors.forename: E
authors.initials: E
authors.lastname: Li
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli; D005461:Fluorine; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D012177:Retinol-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A / Q000378:metabolism*
chemical_list: D001059:Apoproteins; D008081:Liposomes; D011994:Recombinant Proteins; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A; D005461:Fluorine