title: Do sheep really have problems with cardiopulmonary bypass for total artificial heart implantation?
abstract: Although the use of sheep in total artificial heart (TAH) implantation has many advantages, they are known to show a significant morbidity rate on cardiopulmonary bypass (CPB); this has been considered to be a major limiting factor in using them for TAH experiments. We conducted a series of ovine CPB experiments to evaluate the sheep's pathophysiological response to CPB. CPB-related hemolysis, bleeding, and lung dysfunction were analyzed in 5 sheep, which had undergone CPB, used at our hospital for TAH implantation. Four of the 5 sheep survived the experimental procedures, and 3 of them survived on a long-term basis. Unacceptable degrees of hemolysis related to CPB were not observed. Postoperative bleeding was not remarkable, and coagulation test results did not show significant abnormal findings. Acute lung injuries of a mild to moderate degree were found mainly at the microscopic level, but rarely had clinical significance. In conclusion, this experiment suggests that sheep can be used for the animal model for TAH implantation with acceptable risk on CPB circuits and techniques are used.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Korea.
authors.forename: W G
authors.initials: WG
authors.lastname: Kim
authors.forename: Y T
authors.initials: YT
authors.lastname: Kim
authors.forename: S K
authors.initials: SK
authors.lastname: Park
authors.forename: H C
authors.initials: HC
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Suh
authors.forename: J W
authors.initials: JW
authors.lastname: Park
authors.forename: W K
authors.initials: WK
authors.lastname: Paik
authors.forename: B H
authors.initials: BH
authors.lastname: Lee
authors.forename: B G
authors.initials: BG
authors.lastname: Min
authors.forename: J R
authors.initials: JR
authors.lastname: Rho
mesh_terms: D015104:3,4-Methylenedioxyamphetamine / Q000097:blood; D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects / Q000662:veterinary*; D005260:Female; D006354:Heart, Artificial; D006461:Hemolysis; D006470:Hemorrhage / Q000209:etiology / Q000662:veterinary; D008168:Lung / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D011183:Postoperative Complications / Q000662:veterinary; D018570:Risk Assessment; D012756:Sheep / Q000502:physiology / Q000601:surgery*; D012757:Sheep Diseases / Q000209:etiology; D013927:Thrombosis / Q000209:etiology / Q000662:veterinary; D001620:beta-Thromboglobulin / Q000032:analysis
chemical_list: D001620:beta-Thromboglobulin; D015104:3,4-Methylenedioxyamphetamine