Pathophysiological and histomorphological evaluation of polyacryloylmorpholine vs polyethylene glycol modified superoxide dismutase in a rat model of ischaemia/reperfusion injury.
Twenty Wistar rats were divided into two groups. Both underwent acute ischaemia followed by reperfusion of the left hind limb. The first group was a control group while the second was treated with PAcM-SOD. The survival percentage of the limb after 10 days was 30% for the first group and 70% for the second. Neither linear regression nor correlation were found between groups as far as the survival percentage of the limb after 10 days and reperfusion pmO2 data were concerned. After ten days the histomorphological analysis was significant regarding the fibre diameter and the percentage of central located nuclei in the specimens of PAcM-SOD treated limbs compared to normal limbs, but not when compared to the muscular fibres of the control group. Comparing these results with others obtained with native SOD and monomethoxypoly(ethylene glycol) modified SOD (mPEG-SOD) used in the same experimental model, we can conclude that the clinical and morphological results were better using mPEG-SOD, and that PAcM-SOD does have a protective effect on ischaemic muscle damage, although it is not as effective as mPEG-SOD in preventing ischaemia/reperfusion injury.
Rocca
M
M
Department of Experimental Surgery, Istituto di Ricerca Codivilla-Putti I.O.R. and Surgical Pathophysiology, Medical School, University of Bologna, Italy.
Giavaresi
G
G
Caliceti
P
P
Veronese
F M
FM
Giardino
R
R
D000180:Acrylic Resins / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D006614:Hindlimb / Q000098:blood supply / Q000473:pathology; D016014:Linear Models; D010101:Oxygen Consumption / Q000502:physiology; D010313:Partial Pressure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000627:therapeutic use*
D000180:Acrylic Resins; C015460:poly(acryloylmorpholine); D011092:Polyethylene Glycols; D013482:Superoxide Dismutase