Structural studies of the acidic exopolysaccharide produced by a mucoid strain of Burkholderia cepacia, isolated from cystic fibrosis.
The acidic exopolysaccharide produced by a mucoid strain of Burkholderia cepacia isolated from a cystic fibrosis patient, was purified by cetyltrimethylammonium bromide precipitation and/or anion-exchange chromatography. Based on the sugar composition and permethylation analyses, supported by GLC-MS and NMR spectroscopy analyses, the repeating-unit of the polysaccharide was established as -->3)-beta-D-Glcp-(1-->3)-[4,6-O-(1-carboxyethylidene)]-alpha-D-Gal p-(1-->.
Cérantola
S
S
Département des Glycoconjugués et Biomembranes, Laboratoire de Pharmacologie et Toxicologie Fondamentales du CNRS, Toulouse, France.
Marty
N
N
Montrozier
H
H
D016956:Burkholderia cepacia / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003550:Cystic Fibrosis / Q000382:microbiology*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006025:Glycosaminoglycans / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D019289:Pyruvic Acid / Q000032:analysis
D006025:Glycosaminoglycans; D011135:Polysaccharides, Bacterial; C046129:mucoid exopolysaccharide (P aeruginosa); D019289:Pyruvic Acid; D005947:Glucose; D005690:Galactose