Lymphocyte adhesive interactions with cultured parotid salivary gland epithelial cells from rats.
Cellular interactions control lymphocyte localization within salivary gland tissues and contribute to the immune defense of oral surfaces. We examined lymphocyte adherence to cultured parotid cells using an in vitro assay and found good correlation with previously reported binding to parotid gland frozen sections. Thoracic duct lymphocytes (TDL) bound to parotid cells in greater numbers than thymocytes (74 vs 11 cells/mm2). B cells showed preferential adherence compared to T cells (75% vs 28%). TDL binding was inhibited by sodium azide or cytochalasin B (60% and 80%, respectively). EDTA inhibition (63%) was restored by replacing calcium (9%) but not magnesium (65%). Binding was inhibited by fucoidin or phosphomannan (approximately 70%). Fibronectin peptides had no effect. Culture supernatants were inhibitory for TDL adherence (60%), suggesting that molecules involved in lymphocyte localization may be shed and that parotid cell cultures will be useful for ligand isolation and characterization.
O'Sullivan
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Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Montgomery
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PC
D000818:Animals; D001386:Azides / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008351:Mannans / Q000494:pharmacology; D010306:Parotid Gland / Q000166:cytology / Q000276:immunology*; D011134:Polysaccharides / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D019810:Sodium Azide; D013897:Thoracic Duct / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology
D001386:Azides; D015815:Cell Adhesion Molecules; D008351:Mannans; D011134:Polysaccharides; D003571:Cytochalasin B; C005437:phosphomannan; C007789:fucoidan; D019810:Sodium Azide; D004492:Edetic Acid