title: Rhesus macaques previously infected with simian/human immunodeficiency virus are protected from vaginal challenge with pathogenic SIVmac239.
abstract: Nontraumatic vaginal inoculation of rhesus macaques with a simian/human immunodeficiency virus (SIV/HIV) chimera containing the envelope gene from HIV-1 89.6 (SHIV 89.6) results in systemic infection (Y. Lu, B. Brosio, M. Lafaile, J. Li, R. G. Collman, J. Sodroski, and C. J. Miller, J. Virol. 70:3045-3050, 1996). A total of five rhesus macaques have each been infected by exposure to at least three intravaginal inoculations of SHIV 89.6. The SHIV 89.6 infection is characterized by a transient viremia that evokes humoral and cellular immune responses to HIV and SIV antigens, but disease does not develop in animals infected with SHIV 89.6. To determine if a previous infection with SHIV 89.6 by vaginal inoculation could protect animals from vaginal challenge with pathogenic SIV, all five animals were intravaginally inoculated twice with pathogenic SIV-mac239. After challenge, all of the SHIV-immunized animals had low or undetectable viral RNA levels in plasma compared to control animals. Three of the five of the SHIV-immunized animals remained virus isolation negative for more than 8 months, while two became virus isolation positive. The presence of SIV Gag-specific cytotoxic T lymphocytes in peripheral blood mononuclear cells and SIV-specific antibodies in cervicovaginal secretions at the time of challenge was associated with resistance to pathogenic SIV infection after vaginal challenge. These results suggest that protection from sexual transmission of HIV may be possible by effectively stimulating both humoral and cellular antiviral immunity in the systemic and genital mucosal immune compartments.
authors.affiliation: California Regional Primate Research Center, Department of Pathology, School of Veterinary Medicine, University of California Davis, 95616, USA. cjmiller@ucdavis.edu
authors.forename: C J
authors.initials: CJ
authors.lastname: Miller
authors.forename: M B
authors.initials: MB
authors.lastname: McChesney
authors.forename: X
authors.initials: X
authors.lastname: Lü
authors.forename: P J
authors.initials: PJ
authors.lastname: Dailey
authors.forename: C
authors.initials: C
authors.lastname: Chutkowski
authors.forename: D
authors.initials: D
authors.lastname: Lu
authors.forename: P
authors.initials: P
authors.lastname: Brosio
authors.forename: B
authors.initials: B
authors.lastname: Roberts
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015483:HIV Antibodies / Q000032:analysis; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008253:Macaca mulatta; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D012367:RNA, Viral / Q000097:blood; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014621:Vagina
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D012367:RNA, Viral