Capillary zone electrophoresis of oligosaccharides derivatized with N-(4-aminobenzoyl)-L-glutamic acid for ultraviolet absorbance detection.
A charged and strongly UV-absorbing tag, N-(4-aminobenzoyl)-L-glutamic acid) (ABG), was coupled to oligosaccharides by reductive amination under mild conditions. The effectiveness of ABG as a derivatization agent is shown through the separation of isomaltooligosaccharides from a dextran hydrolysate. The minimum detectable quantities in the subpicomole range are demonstrated.
Plocek
J
J
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Novotny
M V
MV
D000586:Amination; D003911:Dextrans / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005971:Glutamates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D003911:Dextrans; D005971:Glutamates; D009844:Oligosaccharides; C011398:4-aminobenzoylglutamic acid