Effects and mechanisms of environmental temperature on carp (Cyprinus carpio) anti-DNP antibody response and non-specific cytotoxic cell activity: a kinetic study.
The effect of environmental temperatures on immune competence was investigated in carp which were subjected to changes in water temperature. The activity of non-specific cytotoxic cells (NCC) against P815 target cells, and the anti-DNP antibody response were evaluated until day 56 after transfer. Low environmental temperature (12 +/- 0.5 degrees C) enhanced NCC activity and decreased antibody production. In contrast a high environmental temperature (28 +/- 0.5 degrees C) was without effect on these parameters when compared to the standard temperature (20 +/- 0.5 degrees C). The results showed a maximum effect of low environmental temperature on day 28 and an adaptation in these immune responses 56 days following transfer. Collectively, the results indicated that non-specific immunity tends to offset specific immune suppression at low environmental temperatures. To determine the mechanism(s) by which environmental temperature affects cellular immune function, membrane fluidity measurements and sialic acid titration, as well as stress assessment by plasma cortisol measurement, were determined on day 28. Taken together, the results revealed a direct effect of temperature on cellular immune function which is modulated by membrane fluidity and sugar concentration and not by stress induction.
Le Morvan
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C
Laboratory of General and Comparative Immunophysiology, UER of Sciences, Limoges, France.
Deschaux
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P
Troutaud
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D
D019297:2,4-Dinitrophenol / Q000276:immunology; D000818:Animals; D000917:Antibody Formation / Q000276:immunology* / Q000528:radiation effects*; D002347:Carps / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000276:immunology* / Q000528:radiation effects*; D004140:Dinitrophenols / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000528:radiation effects; D007700:Kinetics; D013696:Temperature
D004140:Dinitrophenols; D019297:2,4-Dinitrophenol