Endogenous alkaloids in man. XXVI. Determination of the dopaminergic neurotoxin 1-trichloromethyl-1,2,3,4-tetrahydro-beta-carboline (TaClo) in biological samples using gas chromatography with selected ion monitoring.
Highly chlorinated beta-carbolines have a potential in vivo relevance to Parkinson's disease. In this paper, a gas chromatographic method for the determination of the neurotoxic 1-trichloromethyl-1,2,3,4-tetrahydro-beta-carboline (TaClo), the condensation product of tryptamine and chloral hydrate, is described. The specific and sensitive assay involves purification of the biological samples by solid-phase extraction with C18 cartridges, derivatization with heptafluorobutyric anhydride, and chromatography on a non-polar fused-silica capillary column. Detection of TaClo was achieved by the registration of characteristic mass fragments of the TaClo heptafluorobutyric amide derivative using selected ion monitoring. The method was utilized to detect and quantify TaClo in blood, urine, bile, faeces, and brain tissue of rats treated with this alkaloid-type heterocycle. Four-fold deuterium-labelled TaClo was used as an internal standard.
Bringmann
G
G
Institute of Organic Chemistry, University of Würzburg, Germany.
Friedrich
H
H
Birner
G
G
Koob
M
M
Sontag
K H
KH
Heim
C
C
Kolasiewicz
W
W
Fähr
S
S
Stäblein
M
M
God
R
R
Feineis
D
D
D000818:Animals; D002243:Carbolines / Q000032:analysis* / Q000493:pharmacokinetics / Q000633:toxicity; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D009420:Nervous System / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D002243:Carbolines; C067372:1-trichloromethyl-1,2,3,4-tetrahydro-beta-carboline