title: High affinity iron acquisition in Rhizobium leguminosarum requires the cycHJKL operon and the feuPQ gene products, which belong to the family of two-component transcriptional regulators.
abstract: The cycHJKL operon of Rhizobium leguminosarum has previously been shown to be involved in the maturation of cytochrome c, possibly by its involvement in the covalent attachment of haem to the apoprotein. Mutations in the cycHJKL genes abolish symbiotic nitrogen fixation. Here, we show that cyc mutants are pleiotropically defective. They have lost a high affinity iron acquisition system due to their failure to make or to export siderophores. They also accumulate protoporphyrin IX, the immediate precursor of haem. A model to account for these phenotypes is presented. Immediately upstream of cycH is a gene, lipA, which is predicted to encode an outer-membrane lipoprotein. Further upstream of lipA, there are two other genes, whose products are similar in sequence to the widespread family of two-component transcriptional regulators. These two genes, feuP and feuQ, did not affect the transcription of lipA, or of the cycHJKL operon. However, a mutation in feuQ also led to the loss of the high affinity iron uptake system, although siderophores were still produced.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
authors.forename: Kay H
authors.initials: KH
authors.lastname: Yeoman
authors.affiliation: John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
authors.forename: Maria-Jesus
authors.initials: MJ
authors.lastname: Delgado
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
authors.forename: Margaret
authors.initials: M
authors.lastname: Wexler
authors.affiliation: John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
authors.forename: J Allan
authors.initials: JA
authors.lastname: Downie
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
authors.forename: Andrew W B
authors.initials: AWB
authors.lastname: Johnston
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D010641:Phenotype; D016961:Rhizobium leguminosarum / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C093104:CycJ protein, Bacteria; C093105:CycK protein, Bacteria; C104567:FeuP protein, bacteria; C104568:FeuQ protein, bacteria; D008565:Membrane Proteins; D014157:Transcription Factors; D007501:Iron