title: Determination of 2-keto-3-deoxyoctulosonic acid (KDO) with high-performance anion-exchange chromatography (HPAEC): survey of stability of KDO and optimal hydrolytic conditions.
abstract: A new method for 2-keto-3-deoxy-octulosonic acid (KDO) determination using high-performance anion-exchange chromatography was developed. KDO is well separated from Neu5Ac, and the response was linear from 20 pmol to 5 nmol. The method was used to examine the stability of KDO under various hydrolytic, conditions and to survey optimal hydrolytic conditions for release of KDO.
authors.affiliation: Biology Department, Johns Hopkins University, Baltimore, Maryland 21218, USA.
authors.forename: J
authors.initials: J
authors.lastname: Kiang
authors.forename: S C
authors.initials: SC
authors.lastname: Szu
authors.forename: L X
authors.initials: LX
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Tang
authors.forename: Y C
authors.initials: YC
authors.lastname: Lee
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000378:metabolism; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010504:Periodic Acid; D012475:Salmonella; D013400:Sugar Acids / Q000032:analysis*
chemical_list: D008070:Lipopolysaccharides; D013400:Sugar Acids; D010504:Periodic Acid; C002532:2-keto-3-deoxyoctonate; C009288:metaperiodate