Cloning and sequence of a gene for a homologue of the C subunit of the V-ATPase from the salivary gland of the tick Amblyomma americanum (L).
A 1084 base pair partial cDNA showing similarity to the C subunit of the vacuolar ATPase (V-ATPase) was isolated on a clone from a cDNA library made from salivary glands from 3-day-old feeding adult Amblyomma americanum (L.) female ticks. The 5' end was completed using primer extension and the two pieces joined to form a complete cDNA of 1373 bp. This mRNA is expressed in embryos and the salivary glands of unfed adults and adult females at all stages of feeding. Specific inhibitors of the V-ATPase decrease the rate of dopamine-stimulated secretion of isolated salivary glands, but not as much as ouabain, an inhibitor of the Na+, K+ ATPase, indicating that a V-ATPase may participate in the mechanism of salivary fluid secretion in A. americanum, but the volume of saliva secreted is more dependent on an active Na+, K+ ATPase.
McSwain
J L
JL
Department of Entomology, Oklahoma State University, Stillwater 74078, USA.
Luo
C
C
deSilva
G A
GA
Palmer
M J
MJ
Tucker
J S
JS
Sauer
J R
JR
Essenberg
R C
RC
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D018942:Macrolides; D008969:Molecular Sequence Data; D010042:Ouabain / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000235:genetics*; D012469:Salivary Glands / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013987:Ticks / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D025262:Vacuolar Proton-Translocating ATPases
D000900:Anti-Bacterial Agents; D018076:DNA, Complementary; D018942:Macrolides; C057620:bafilomycin A; D010042:Ouabain; C036978:concanamycin A; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D005033:Ethylmaleimide