title: Phenotypic expression of a mannose-sensitive hemagglutinin by a Vibrio cholerae O1 E1Tor strain and evaluation of its role in intestinal adherence and colonization.
abstract: A Vibrio cholerae O1 strain (1150) of the EIT or biotype and Ogawa serotype with haemagglutination (HA) activity was subjected to TnphoA mutagenesis. Out of several mutants isolated, one HA- and another HA+ mutant were further characterised. The HA- mutant showed about 50% reduction in its intestinal adherence capacity in vitro and about 9-fold decrease of its colonisation ability in vivo, as compared to the wild-type strain. Subsequent studies showed that the HA activity of strain 1150 was mediated by a mannose-sensitive haemagglutinin (MSHA). Thus, the phenotypic expression of MSHA appears to be partly responsible for the intestinal adherence and colonisation properties of strain 1150.
authors.affiliation: Department of Microbiology, Bose Institute, Calcutta, India.
authors.forename: S
authors.initials: S
authors.lastname: Mukhopadhyay
authors.forename: C
authors.initials: C
authors.lastname: Ghosh
authors.forename: A C
authors.initials: AC
authors.lastname: Ghose
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D015686:Gene Products, env / Q000235:genetics / Q000502:physiology; D006388:Hemagglutinins / Q000235:genetics / Q000502:physiology*; D066298:In Vitro Techniques; D007422:Intestines / Q000382:microbiology; D008358:Mannose / Q000494:pharmacology; D051379:Mice; D009154:Mutation; D010641:Phenotype; D011817:Rabbits; D014734:Vibrio cholerae / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*
chemical_list: D015686:Gene Products, env; D006388:Hemagglutinins; D008358:Mannose