A rapid serological detection method for squamous cell carcinoma.
OBJECTIVE
We conjugated the squamous cell carcinoma (CA)-associated antibody (anti-YM antibody) with gelatin particles. We found that these particles reacted specifically with the tumor-associated antigen (YM antigen) in serum samples from patients with squamous cell CA of the uterine cervix and various organs (GPA reaction).


METHODS
1) We performed immunohistochemical studies of the YM antigen in dysplasia and invasive squamous cell CA of the uterine cervix; 2) We measured the GPA reaction in various malignant and non-malignant gynecologic diseases.


RESULTS
1) Immunohistochemical localization of the YM antigen was over 75% in cases of dysplasia and invasive squamous cell CA of the uterine cervix, and was 21% in normal squamous epithelium of the uterine cervix. 2) The GPA reaction in gynecologic diseases was positive in 57.1% of the dysplasia cases; in CIS cases, 69.2%; in cervical CA, 83.0%; in corpus CA, 25.0%; in leiomyoma 16.7%; and in pregnant serum samples, 66.7%, 3) GPA reactions in non-ob-gyn cases were positive in 68.8% of the cases of squamous cell CA of the head and neck; 66.7% in cases of squamous cell CA of the lung; and 25.0% in adenoCA of the lungs whereas the sera of patients with benign tumors and of healthy persons were free of the YM antigen.


CONCLUSIONS
We concluded that this newly developed GPA-reaction measurement is a simple and reliable serologic test for detecting the initial stage of squamous cell CA of various organs and for monitoring its postoperative status.
Yoshizawa
H
H
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa, Japan.
Yamauchi
S
S
Kenjo
T
T
Kuroshima
Y
Y
Makino
T
T
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000912:Antibodies, Neoplasm / Q000302:isolation & purification; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000302:isolation & purification; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D005260:Female; D006258:Head and Neck Neoplasms / Q000175:diagnosis; D006801:Humans; D011247:Pregnancy; D011817:Rabbits; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor