Macrophage recognition of LDL modified by levuglandin E2, an oxidation product of arachidonic acid.
Levuglandin (LG) E2, a secoprostanoic acid levulinaldehyde derivative, is a product of free radical oxidation that forms covalent adducts with lysyl residues on proteins. Treatment of LDL with LGE2 leads to uptake and degradation by mouse peritoneal macrophages. Oxidized LDL, but not acetyl LDL efficiently competed for binding and uptake of LGE2-modified 125I-LDL. This result suggests that LGE2-modified LDL was recognized by a class of scavenger receptor that demonstrated ligand specificity for oxidized LDL but not for acetyl LDL.
Hoppe
G
G
Department of Cell Biology, Research Institute, Cleveland Clinic Foundation, OH 44195, USA.
Subbanagounder
G
G
O'Neil
J
J
Salomon
R G
RG
Hoff
H F
HF
D000107:Acetylation; D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001665:Binding Sites; D016166:Free Radical Scavengers / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008315:Malondialdehyde / Q000494:pharmacology; D051379:Mice; D008956:Models, Chemical; D015394:Molecular Structure; D011458:Prostaglandins E / Q000494:pharmacology*
D000447:Aldehydes; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D011458:Prostaglandins E; C099252:oxidized low density lipoprotein; D016718:Arachidonic Acid; D008315:Malondialdehyde; C047684:levuglandin E2; C027576:4-hydroxy-2-nonenal