Cloning and characterization of a cotton lipid transfer protein gene specifically expressed in fiber cells.
A cotton genomic library was screened using a fiber-specific cDNA (GH3) encoding a lipid transfer protein (LTP). One genomic clone (1.7 kb DNA insert) containing the Ltp gene (Ltp6) was sequenced and characterized. The Ltp6 contains an open reading frame of 360 bp, which is interrupted by a single intron (136 bp) located in the region corresponding to the C-terminal of the protein. The derived amino-acid sequence of LTP6 is 64% homologous to that of GH3. Like the GH3 gene, the Ltp6 is specifically expressed in fiber cells in a temporal manner. However, its expression level is lower than that of GH3.
Ma
D P
DP
Department of Biochemistry and Molecular Biology, Mississippi State University 39762 USA. dml@ra.msstate.edu
Liu
H C
HC
Tan
H
H
Creech
R G
RG
Jenkins
J N
JN
Chang
Y F
YF
D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D003368:Gossypium / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D030262:Soybean Proteins
D052179:Antigens, Plant; D002352:Carrier Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; C070797:GH3 protein, soybean; D010940:Plant Proteins; D030262:Soybean Proteins; C511920:lipid transfer proteins, plant