Is the CvaA protein, encoded within the colicin V export gene cvaA, required for colicin V transport?
The antibacterial peptide toxin colicin V is exported from Escherichia coli cells by a signal sequence-independent, ABC export system. Export requires at least three proteins-membrane fusion protein CvaA, ABC export protein CvaB, and outer membrane protein TolC. The cvaA gene also encodes a second protein, CvaA, initiated from an in-frame translational re-start within the cvaA coding sequence. To determine whether the internally encoded CvaA protein also functions in the export pathway, the putative start codons for CvaA were mutagenized, while maintaining CvaA function. Elimination of CvaA translation caused no change in colicin V export levels, indicating that the CvaA protein is not required in the secretion pathway.
Skvirsky
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Department of Biology, University of Massachusetts Boston 02125, USA. skvirsky@umbsky.cc.umb.edu
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D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001693:Biological Transport, Active; D003087:Colicins / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D003087:Colicins; C096447:CvaA protein, E coli; D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D008565:Membrane Proteins