title: Inhibitory effects of caffeine on Ca2+ influx and histamine secretion independent of cAMP in rat peritoneal mast cells.
abstract: 1. Caffeine did not evoke Ca2+ mobilization and histamine secretion. 2. Caffeine, as well as other methylxanthines but not forskolin or 8 bromo-cAMP, inhibited Ca2+ responses from compound 48/80. 3. Evoked histamine secretion was severely reduced by caffeine but not by cAMP analogs. 4. In beta-escin-permeabilized cells, caffeine did not affect resting and IP3-stimulated 45Ca2+ release, but it inhibited Ca(2+)-induced histamine secretion. 5. These results indicate that caffeine inhibits Ca2+ influx and Ca2+ efficacy in the secretory apparatus independent of cAMP, resulting in the inhibition of secretagogs-evoked histamine secretion from rat mast cells.
authors.affiliation: Department of Toxicology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Teraoka
authors.forename: H
authors.initials: H
authors.lastname: Akiba
authors.forename: R
authors.initials: R
authors.lastname: Takai
authors.forename: T
authors.initials: T
authors.lastname: Taneike
authors.forename: T
authors.initials: T
authors.lastname: Hiraga
authors.forename: A
authors.initials: A
authors.lastname: Ohga
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability; D000242:Cyclic AMP / Q000494:pharmacology*; D004928:Escin; D006636:Histamine Release / Q000187:drug effects*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D014970:Xanthines / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D014970:Xanthines; D002110:Caffeine; D003189:p-Methoxy-N-methylphenethylamine; D004928:Escin; D000242:Cyclic AMP; D002118:Calcium