Slide-based laser scanning cytometry.
OBJECTIVE
To show that laser scanning cytometry (LSCM) can provide data equivalent to flow cytometry (FCM) data and furnish a number of benefits, including cell relocation for visualization and several additional measurement features that may make it more suitable than FCM for pathology laboratories.


METHODS
A laser scanning cytometer, the LSC, was developed. Several instruments, at sites in the United States and Japan during the last two years, provided data characterizing the instrument and its usefulness.


RESULTS
Data describing the sensitivity, precision, accuracy, utility of added measurement features and cell relocation capabilities of the LSC are presented. The data illustrate the applicability of the LSC to multiparameter DNA ploidy studies, resolution of phases of the cell cycle and cytogenetics.


CONCLUSIONS
Because it is microscope based and measures cells on a slide, not in a flow chamber; records the position of each cell on the slide; and has higher resolution, LSCM provides a number of benefits that may make it more suitable than FCM for pathology laboratories.
Kamentsky
L A
LA
CompuCyte Corporation, Cambridge, Massachusetts 02139, USA.
Burger
D E
DE
Gershman
R J
RJ
Kamentsky
L D
LD
Luther
E
E
D000818:Animals; D002453:Cell Cycle; D002843:Chromatin / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis; D004867:Equipment Design; D005470:Fluorometry / Q000295:instrumentation*; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation*; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation; D007834:Lasers; D008853:Microscopy / Q000295:instrumentation*; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology; D011003:Ploidies; D012680:Sensitivity and Specificity
D002843:Chromatin; D004273:DNA, Neoplasm