title: Beet soil-borne virus RNA 2: similarities and dissimilarities to the coat protein gene-carrying RNAs of other furoviruses.
abstract: The complete sequence of the 3454 nt of RNA 2 of the Ahlum isolate of beet soil-borne furovirus (BSBV) has been determined starting with two short stretches of cloned cDNA. Unknown parts of the sequence were amplified by means of RT-PCR techniques using combinations of specific and random primers. BSBV RNA 2 is more similar in its genetic organization to potato mop top virus (PMTV) RNA 3 than to any other furoviral RNA, although it is more than 1100 nt longer. Its 3'-end, unlike that of PMTV RNA 3, has the potential to fold into a tRNA-like structure. It contains one large open reading frame for a readthrough protein with a molecular mass of 104 kDa (104K protein) which is interrupted internally by an amber stop codon terminating the coding region for a protein of 19 kDa (19K), most likely the viral coat protein (CP). The readthrough domain of the 104K protein is much larger than that of PMTV, but the N- and C-proximal portions of these domains are similar for the two viruses. No serological relationships were found between the particles of the two viruses, although more than 50% of the amino acid sequences of the putative CPs are identical.
authors.affiliation: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut fürBiochemie und Pflanzenvirologie, Braunschweig, Germany. r.koenig@bba.d400.de
authors.forename: R
authors.initials: R
authors.lastname: Koenig
authors.forename: U
authors.initials: U
authors.lastname: Commandeur
authors.forename: S
authors.initials: S
authors.lastname: Loss
authors.forename: C
authors.initials: C
authors.lastname: Beier
authors.forename: A
authors.initials: A
authors.lastname: Kaufmann
authors.forename: D E
authors.initials: DE
authors.lastname: Lesemann
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010942:Plant Viruses / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D014675:Vegetables / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; D014764:Viral Proteins; C103974:coat protein, Beet soil-borne virus