Proliferation of smooth muscle cells in acute allograft vascular rejection.
OBJECTIVE
To determine whether immune injury during acute cardiac rejection induces phenotypic modulation of arterial smooth muscle cells and lesion formation, we studied the expression of embryonic myosin heavy-chain isoform and degrees of intimal proliferation in aortas and coronary arteries of allografted rabbit hearts. Modulation of phenotype in arterial smooth muscle cells during acute vascular injury is a widely reported phenomenon, and proliferation and migration of medial smooth muscle cells contribute to development of intimal hyperplasia of arteries in response to immune injury.


METHODS
Rabbit hearts were heterotopically transplanted to the neck without immunosuppression. Hearts were harvested at 2, 5, 7, and 10 days after transplantation. Proliferation of smooth muscle cells was assessed by bromodeoxyuridine labeling. Staining for immunohistochemical indicators was done with use of monoclonal antibodies that recognize T lymphocytes and all types of smooth muscle cells (SM1), adult type of smooth muscle cells (SM2), and embryonic myosin heavy-chain isoform. Intimal thickening and luminal narrowing were assessed with a computer-assisted video image analysis system.


RESULTS
Intimal thickening and luminal narrowing in aortas and coronary arteries gradually increased in a time-dependent manner. The neointima thus formed consisted of proliferating smooth muscle cells positive for both SM1 and embryonic myosin heavy-chain isoform and massive T lymphocyte accumulation. Intimal proliferation was more prominent in aortas and large epicardial coronary arteries than in the intramyocardial small coronary arteries.


CONCLUSIONS
These findings suggest that allogeneic immune injury facilitates phenotypic modulation of smooth muscle cells, which may contribute to subsequent transplantation-associated atherosclerosis.
Amano
J
J
Second Department of Surgery, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
Ishiyama
S
S
Nishikawa
T
T
Tanaka
H
H
Nagai
R
R
Marumo
F
F
Hiroe
M
M
D000818:Animals; D001011:Aorta / Q000166:cytology*; D002455:Cell Division; D003331:Coronary Vessels / Q000166:cytology*; D006084:Graft Rejection; D016027:Heart Transplantation; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011817:Rabbits