[Molecular characterization of HTLV-II from an intravenous drug addict with AIDS in Argentina].
Ten intravenous drug user AIDS patients were studied to determine the presence of HTLV-I/II infection. Sera were screened by particle agglutination test and by an in house indirect immunofluorescence assay using MT2 (HTLV-I) and C19 (HTLV-II) producing cell lines. Out of the ten sera, one was confirmed HTLV-II positive by Western blot. Peripheral blood mononuclear cells were obtained from this patient, separated through a Ficoll-Hypaque gradient and DNA was extracted and amplified by semi-nested PCR. The amplified product obtained was cloned and sequenced. Results demonstrated the presence of HTLV-II proviral DNA and comparison of the obtained sequence with different HTLV-II prototypes led to classify the virus into subtype a. This is the first molecular characterization of HTLV-II in an intravenous drug user in Argentina.
Biglione
M
M
Centro Nacional de Referencia para el SIDA, Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Avila
M M
MM
Biglione
J
J
Weisburd
G
G
Libonatti
O
O
Gessain
A
A
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000328:Adult; D001118:Argentina; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000302:isolation & purification; D015491:HTLV-II Infections / Q000150:complications* / Q000821:virology; D015367:Human T-lymphotropic virus 2 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D015819:Substance Abuse, Intravenous / Q000150:complications*
D004279:DNA, Viral