Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice.
We examined the pathogenetic mechanism underlying the lack of bone resorption in osteosclerotic oc/oc mice. An immunoelectron microscopic analysis revealed that in the osteoclasts of these mice, no ruffled borders formed, and that vacuolar H+-ATPase (V-ATPase) was present throughout the cytoplasm but not on the apical membranes. The activity of V-ATPase in oc/oc mice was similar to that in normal mice. In normal spleen cell-derived osteoclast-like cells (OCLs), immunoreactivity for V-ATPase was detected in association with Triton X-100-insoluble cellular structure, but not in oc/oc spleen cell-derived OCLs. Moreover, in renal proximal convoluted tubules of oc/oc mice, the basal striation did not form. These results suggest that osteosclerosis in oc/oc mice is possibly due to the dissociation of V-ATPase and cytoskeleton in osteoclasts.
Nakamura
I
I
Department of Biochemistry, School of Dentistry, Showa University, Shinagawa-ku, Tokyo, Japan.
Takahashi
N
N
Udagawa
N
N
Moriyama
Y
Y
Kurokawa
T
T
Jimi
E
E
Sasaki
T
T
Suda
T
T
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001862:Bone Resorption; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000201:enzymology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000201:enzymology* / Q000648:ultrastructure; D010026:Osteosclerosis / Q000201:enzymology*; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D014617:Vacuoles / Q000201:enzymology
D000251:Adenosine Triphosphatases