title: Antibody mediated ligation of platelet/endothelial cell adhesion molecule-1 (PECAM-1) on neutrophils enhances adhesion to cultured human dermal microvascular endothelial cells.
abstract: We investigated the effects on leukocyte adhesion to cultured human dermal microvascular endothelial (DMvE) cell by antibody mediated ligation of platelet/endothelial cell adhesion molecule-1 (PECAM-1) on neutrophils. Preincubation of neutrophils with anti-PECAM-1 antibody markedly enhanced their adhesion to DMvE cells, while the adhesion-increasing action was cancelled out by anti-CR3 or anti-LFA-1 beta (CD18) antibody. These findings suggest that ligation of PECAM-1 on neutrophils increases their adhesion to cultured DMvE cells depending on the beta 2 integrin family, and PECAM-1 may have a crucial role in dermal inflammation.
authors.affiliation: Department of Pathology, Kagawa Medical University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kuwabara
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
authors.forename: M
authors.initials: M
authors.lastname: Oryu
authors.forename: H
authors.initials: H
authors.lastname: Uda
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D002448:Cell Adhesion / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D006801:Humans; D008854:Microscopy, Electron; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D019408:Platelet Endothelial Cell Adhesion Molecule-1