title: Molecular structure of the sarcomeric M band: mapping of titin and myosin binding domains in myomesin and the identification of a potential regulatory phosphorylation site in myomesin.
abstract: The M band of sarcomeric muscle is a highly complex structure which contributes to the maintenance of the regular lattice of thick filaments. We propose that the spatial coordination of this assembly is regulated by specific interactions of myosin filaments, the M band protein myomesin and the large carboxy-terminal region of titin. Corresponding binding sites between these proteins were identified. Myomesin binds myosin in the central region of light meromyosin (LMM, myosin residues 1506-1674) by its unique amino-terminal domain My1. A single titin immunoglobulin domain, m4, interacts with a myomesin fragment spanning domains My4-My6. This interaction is regulated by phosphorylation of Ser482 in the linker between myomesin domains My4 and My5. Myomesin phosphorylation at this site by cAMP-dependent kinase and similar or identical activities in muscle extracts block the association with titin. We propose that this demonstration of a phosphorylation-controlled interaction in the sarcomeric cytoskeleton is of potential relevance for sarcomere formation and/or turnover. It also reveals how binding affinities of modular proteins can be regulated by modifications of inter-domain linkers.
authors.affiliation: Max-Planck-Institute for Biophysical Chemistry, Department of Biochemistry, Göttingen, Germany.
authors.forename: W M
authors.initials: WM
authors.lastname: Obermann
authors.forename: M
authors.initials: M
authors.lastname: Gautel
authors.forename: K
authors.initials: K
authors.lastname: Weber
authors.forename: D O
authors.initials: DO
authors.lastname: Fürst
mesh_terms: D000409:Alanine; D000818:Animals; D010582:Bacteriophage lambda; D001665:Binding Sites; D002417:Cattle; D064211:Connectin; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D009218:Myosins / Q000378:metabolism*; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D012518:Sarcomeres / Q000737:chemistry*; D012694:Serine
chemical_list: D064211:Connectin; D008565:Membrane Proteins; D009124:Muscle Proteins; D011994:Recombinant Proteins; C577285:TTN protein, human; D012694:Serine; D011494:Protein Kinases; D009218:Myosins; D000409:Alanine