Plasma and erythrocyte vitamin E content in asymptomatic hypercholesterolemic subjects.
The present study was designed to assess plasma and erythrocyte vitamin E concentrations in 57 asymptomatic hypercholesterolemic (HC) men compared with 56 normocholesterolemic (NC) men. Vitamin E concentrations were determined by using a reversed-phase HPLC method. Compared with NC subjects, HC men had a significantly lower red blood cell (RBC) vitamin E content in spite of their normal plasma vitamin E concentration. This study demonstrates that total plasma vitamin E concentration is not a suitable predictor of cell vitamin E status and suggests an abnormal transfer of tocopherol between plasma and RBCs in HC men. Moreover, the RBCs of HC men were more susceptible to a peroxidative stress. The strong correlation between RBC susceptibility to oxidation and RBC vitamin E content suggests that the low RBC vitamin E content found in HC men has physiological consequences on the RBC oxidation.
Simon
E
E
Laboratoire de Biochimie, Hôpital Broussais, Paris, France.
Paul
J L
JL
Soni
T
T
Simon
A
A
Moatti
N
N
D000578:Amidines / Q000494:pharmacology; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D008297:Male; D018384:Oxidative Stress; D012016:Reference Values; D014810:Vitamin E / Q000097:blood*
D000578:Amidines; D008078:Cholesterol, LDL; D014810:Vitamin E; C046728:2,2'-azobis(2-amidinopropane); D002784:Cholesterol