title: One-step optimization using touchdown and stepdown PCR.
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, USA.
authors.forename: K H
authors.initials: KH
authors.lastname: Roux
authors.forename: K H
authors.initials: KH
authors.lastname: Hecker
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D007202:Indicators and Reagents; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase