Expression of a 26S proteasome ATPase subunit, MS73, in muscles that undergo developmentally programmed cell death, and its control by ecdysteroid hormones in the insect Manduca sexta.
MS73, an ATPase regulatory subunit of the 26S proteasome in the moth Manduca sexta, is shown to be expressed at a high level only in muscles that are undergoing developmentally programmed cell death, or which are destined to do so. The amount of MS73 is increased by more than two-fold just before death in each of three different muscles that die at different times, under different developmental controls. An ecdysteroid (moulting hormone) agonist, RH-5849, that prevents the occurrence of programmed cell death in two of these muscles also prevents the normally occurring rise in level of MS73 in these muscles. This evidence is consistent with a role for MS73 in programmed cell death.
Löw
P
P
School of Biology and Biochemistry, University of Bath, Claverton Down, UK.
Bussell
K
K
Dawson
S P
SP
Billett
M A
MA
Mayer
R J
RJ
Reynolds
S E
SE
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004441:Ecdysterone / Q000819:agonists / Q000494:pharmacology*; D006834:Hydrazines / Q000494:pharmacology; D018402:Manduca / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D008675:Metamorphosis, Biological / Q000187:drug effects; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex
D006834:Hydrazines; C056565:1,2-dibenzoyl-tert-butylhydrazine; D004441:Ecdysterone; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; D000251:Adenosine Triphosphatases