Regulation of leptin receptor and NPY gene expression in hypothalamus of leptin-treated obese (ob/ob) and cold-exposed lean mice.
Leptin receptor gene expression has been measured in arcuate and ventromedial hypothalamic nuclei. Receptor mRNA in both hypothalamic areas was higher in obese mice than in lean littermates. Twice daily leptin administration for 7 days profoundly affected food intake, reduced leptin receptor mRNA in the arcuate nucleus, and had a similar effect on neuropeptide Y gene expression. A single leptin injection was ineffective. Exposure of lean mice to cold for 24 h caused an induction of leptin receptor and NPY mRNA which was normalized when animals were returned to the warm. Regulation of receptor gene expression may be an important component in the reading of the leptin signal.
Mercer
J G
JG
Molecular Neuroendocrinology Group, Rowett Research Institute, Aberdeen, UK. jgm@rri.sari.ac.uk
Moar
K M
KM
Rayner
D V
DV
Trayhurn
P
P
Hoggard
N
N
D000064:Acclimatization; D000704:Analysis of Variance; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis*; D003080:Cold Temperature; D005247:Feeding Behavior / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007031:Hypothalamus / Q000378:metabolism*; D017403:In Situ Hybridization; D020738:Leptin; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*; D014697:Ventromedial Hypothalamic Nucleus / Q000378:metabolism
D002352:Carrier Proteins; D020738:Leptin; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D017476:Receptors, Neuropeptide Y; D011994:Recombinant Proteins; C515447:leptin receptor, mouse