Erinacin, an antihaemorrhagic factor from the European hedgehog, Erinaceus europaeus.
An antihaemorrhagic factor named erinacin was purified from the skeletal muscle extract of the European hedgehog, Erinaceus europaeus, by ammonium sulfate precipitation followed by various steps of ion-exchange (DEAE-cellulose), absorption chromatography (hydroxylapatite), and gel filtration (cellofine gel). A 625-fold purification was achieved with an overall yield of 19% antihaemorrhagic activity. The protein effectively inhibited the activity of Bothrops jararaca venom haemorrhagin and did not inhibit the enzymatic activity of trypsin and chymotrypsin. Erinacin is a large molecule (about 1,000,000 mol. wt). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of two subunits: one with an apparent mol. wt of 35,000 forming a larger subunit (350,000) by cross-linking with disulfide bridges, and a second with a mol. wt of 39,000 without disulfides. Dissociation of erinacin into its subunits resulted in complete loss of its antihaemorrhagic activity.
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Zentrum der Rechtsmedizin, University of Frankfurt, Germany.
Omori-Satoh
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Yamakawa
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Nagaoka
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Y
D000818:Animals; D000997:Antivenins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006364:Hedgehogs; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000493:pharmacokinetics / Q000494:pharmacology; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology
D000997:Antivenins; D001798:Blood Proteins; D009124:Muscle Proteins; D011480:Protease Inhibitors; C003941:antihemorrhagic factor; C105466:erinacin protein, Erinaceus europaeus