Therapeutic potential of antiidiotypic single chain antibodies with yeast killer toxin activity.
Single chain fragment (ScFv) antiidiotypic antibodies (antilds) of a killer toxin (KT) from the yeast Pichia anomala have been produced by recombinant DNA methodology from the splenic lymphocytes of mice immunized by idiotypic vaccination with a KT-neutralizing monoclonal antibody (Mab KT4). ScFv KT-like antilds (KTIdAb) react with specific Candida albicans KT cell wall receptors (KTR) exerting a candidacidal activity in vitro could be neutralized by adsorption with Mab KT4. ScFv KTIdAb displayed an effective therapeutic activity in an experimental model of rat candidal vaginitis.
Magliani
W
W
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università degli Studi di Parma, Italy.
Conti
S
S
de Bernardis
F
F
Gerloni
M
M
Bertolotti
D
D
Mozzoni
P
P
Cassone
A
A
Polonelli
L
L
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002176:Candida albicans / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D002181:Candidiasis, Vulvovaginal / Q000188:drug therapy*; D004274:DNA, Recombinant; D015195:Drug Design; D005260:Female; D005818:Genetic Engineering; D000922:Immunotoxins / Q000494:pharmacology / Q000627:therapeutic use*; D055646:Killer Factors, Yeast; D051379:Mice; D008826:Microbial Sensitivity Tests; D009183:Mycotoxins / Q000096:biosynthesis / Q000494:pharmacology / Q000627:therapeutic use*; D010843:Pichia; D051381:Rats
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D004274:DNA, Recombinant; D000922:Immunotoxins; D055646:Killer Factors, Yeast; D009183:Mycotoxins