[The cytogenetic study of established Syrian hamster cell lines. II. A comparative analysis of the karyotypes of cell lines HaK, CER and BHK-21 (C-13)].
A comparative cytogenetic investigation of the Syrian hamster HaK, CER cell lines and BHK-21 (C-13) sublines was undertaken using G- and C-banding, and silver staining of chromosomes. BHK-21 (C-13) cell sublines were found to have diploid associated chromosome sets, whereas HaK and CER cell lines are characterized with triploid and tetraploid associated chromosome sets, resp. In sum, in all the analysed cell lines 100 marker chromosomes were identified, with 85 ones having unique morphology. The finding indicates a nonaccidental appearance of lesions for normal chromosomes in forming markers. Another arrangement of "hot break-points" on chromosomes is more typical of HaK and CER cell lines, than of BHK-21 (C-13) sublines. The localization of "hot break-points" on chromosomes in BHK-21 (C-13) sublines is specific of the Syrian hamster cells transformed with oncogenic viruses.
Urmanova
M A
MA
Tsareva
A A
AA
D000818:Animals; D002460:Cell Line / Q000648:ultrastructure*; D002478:Cells, Cultured; D002642:Chick Embryo; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D006224:Cricetinae; D004622:Embryo, Mammalian; D007621:Karyotyping; D007668:Kidney; D008647:Mesocricetus; D008677:Metaphase