title: Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells.
abstract: A putative second envelope glycoprotein (E2) of hepatitis C virus (HCV) was constitutively produced in a Chinese hamster ovary cell line stably transformed with a plasmid expressing E2 protein under the control of an exogenous promoter and a signal sequence. E2 protein that lacked part of the C-terminal hydrophobic region was glycosylated with high-mannose type oligosaccharides and retained in the cells. On the other hand, E2 protein lacking the entire C-terminal hydrophobic region was glycosylated with complex type oligosaccharides (complex form) and excreted into the culture medium. Immunoreactivity of the high-mannose and complex forms of E2 proteins against sera from HCV infected patients were analyzed. We found that the antigenicity of the complex form of E2 protein was greater than that of the high-mannose form of E2 protein. This result indicated that the complex form of the E2 protein is superior for use in diagnosing HCV infection.
authors.affiliation: Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Inudoh
authors.forename: H
authors.initials: H
authors.lastname: Nyunoya
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Hijikata
authors.forename: N
authors.initials: N
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Shimotohno
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D006031:Glycosylation; D018936:Hepatitis C Antigens / Q000276:immunology*; D008358:Mannose / Q000097:blood; D008403:Mass Screening; D009844:Oligosaccharides / Q000737:chemistry; D010957:Plasmids / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D018936:Hepatitis C Antigens; D009844:Oligosaccharides; D014759:Viral Envelope Proteins; D013762:Tetrahydrofolate Dehydrogenase; D008358:Mannose