Ligand-induced rapid desensitization causes structural alteration of rat ovarian LH/hCG receptor.
The role of the physical state of ovarian membranes was studied in the process of the early desensitization of the LH/hCG receptor. Thirty min after injection of a desensitizing dose of hCG to rats, the hCG-responsive adenylylcyclase activity was reduced, whereas hCG binding to ovarian membranes was still normal. Early desensitization decreased rigidity of membrane lipids, determined by fluorescence polarization of DPH. Possible structure-functional properties of the LH/hCG receptor were analyzed by thermal perturbation technique. Desensitization decreased thermal stability of the LH/hCG receptor in membranes and in proteoliposomes. Desensitization modified the quenching of protein fluorescence and intrinsic fluorescence spectral properties of membranes. The Stern-Volmer constants for control and desensitized membranes were found to be 4.3 and 5.5, respectively, indicating that desensitization elevated the accessibility of fluorophores for acrylamide. The changes of the physical properties of membranes resulting from desensitization was exhibited solely in the treatment with hCG in vivo, but not in vitro. The results suggest that the hCG-induced alteration of the physical state of luteal membranes may be a requirement for the induction of changes that lead to desensitization.
Kolena
J
J
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava; Slovak Republic. Kolena@UEE.SAVBA.SK
Scsuková
S
S
Jezová
M
M
Vranová
J
J
Tatara
M
M
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000378:metabolism / Q000494:pharmacology*; D004161:Diphenylhexatriene; D004355:Drug Stability; D004361:Drug Tolerance; D005260:Female; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006358:Hot Temperature; D010053:Ovary / Q000737:chemistry* / Q000378:metabolism; D011555:Pseudopregnancy; D051381:Rats; D017208:Rats, Wistar; D011974:Receptors, LH / Q000737:chemistry* / Q000187:drug effects*; D013050:Spectrometry, Fluorescence
D006063:Chorionic Gonadotropin; D005456:Fluorescent Dyes; D011974:Receptors, LH; D004161:Diphenylhexatriene; D000262:Adenylyl Cyclases