Nidulal, a novel inducer of differentiation of human promyelocytic leukemia cells from Nidula candida.
Nidulal (1), a novel inducer of differentiation of human HL-60 promyelocytic leukemia cells, was isolated from fermentations of the basidiomycete Nidula candida together with low amounts of niduloic acid (2). Both compounds are bisabolane sesquiterpenes. Their structures were elucidated by spectroscopic methods. In reporter gene assays nidulal (1) preferentially activated the transcription factor complex AP-1-mediated expression of secreted alkaline phosphatase in COS-7 cells. In addition nidulal (1) and niduloic acid (2) exhibited weak cytotoxic and antibiotic activities.
Erkel
G
G
Lehrbereich Biotechnologíe, Universität Kaiserslautern, Germany.
Becker
U
U
Anke
T
T
Sterner
O
O
D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017209:Apoptosis; D001487:Basidiomycota / Q000378:metabolism*; D019556:COS Cells; D002454:Cell Differentiation; D002460:Cell Line; D003509:Cyclohexanecarboxylic Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D053938:DNA Fragmentation; D004353:Drug Evaluation, Preclinical; D005658:Fungi / Q000187:drug effects; D005663:Furans / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009152:Mutagenicity Tests; D012486:Salmonella typhimurium / Q000187:drug effects; D015398:Signal Transduction; D013141:Spiro Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014158:Transcription, Genetic
D000900:Anti-Bacterial Agents; D003509:Cyclohexanecarboxylic Acids; D005663:Furans; D013141:Spiro Compounds; D018808:Transcription Factor AP-1; C104483:nidulal; C104484:niduloic acid; D000469:Alkaline Phosphatase