Modulation of oscillator interactions in the crab stomatogastric ganglion by crustacean cardioactive peptide.
The modulation of the pyloric rhythm of the stomatogastric ganglion of the crab, Cancer borealis, by crustacean cardioactive peptide (CCAP) is described. CCAP activated pyloric rhythms in most silent preparations, and altered the phase relationships of pyloric motor neuron firing in all preparations. In CCAP, the pyloric rhythms were characterized by long lateral pyloric (LP) neuron bursts of action potentials. The threshold for CCAP action was approximately 10(-10) M, with increasing effects at higher CCAP concentrations. The changes in motor pattern evoked by CCAP produced significant changes in LP-innervated muscle movement. These movements were additionally potentiated by CCAP applications to isolated nerve-muscle preparations. Thus, enhanced motor neuron firing and increase of the gain of the neuromuscular junctions are likely to operate coordinately in response to hormonally released CCAP. High CCAP concentrations sometimes resulted in modification of the normal 1:1 alternation between the pyloric dilator (PD) and LP neurons to patterns of 2:1, 3:1, or 4:1 alternation. CCAP seems to activate slow intrinsic oscillations in the LP neuron, as well as enhance faster oscillations in the pacemaker group of PD/anterior burster (AB) neurons. Simulations of fast and slow oscillators with reciprocal inhibitory coupling suggest mechanisms that could account for the mode switch from 1:1 alternation to multiple PD bursts alternating with one LP neuron burst.
Weimann
J M
JM
Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA.
Skiebe
P
P
Heinzel
H G
HG
Soto
C
C
Kopell
N
N
Jorge-Rivera
J C
JC
Marder
E
E
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D003386:Brachyura / Q000502:physiology*; D002116:Calcitonin / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D017952:Ganglia, Invertebrate / Q000187:drug effects* / Q000502:physiology; D005769:Gastrointestinal Motility / Q000187:drug effects*; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009469:Neuromuscular Junction / Q000187:drug effects / Q000502:physiology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology; D010507:Periodicity; D011708:Pylorus / Q000294:innervation*
D010446:Peptide Fragments; C037553:katacalcin; D002116:Calcitonin