title: Clustering of Argentinean tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene.
abstract: The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were made. A partial sequence of the N gene was able to classify our local isolates within three Tospovirus species previously described (Tomato spotted wilt virus, TSWV; tomato chlorotic spot virus, TCSV and groundnut ringspot virus, GRSV). With the sequence data currently available, we performed a cladistic phylogenetic analysis which gave a possible genealogy among members of the Tospovirus genus.
authors.affiliation: Instituto de Bioquímica y Biología Molecular (IBBM), Fac. Cs. Exactas, Universidad Nacional de La Plata, Argentina.
authors.forename: R
authors.initials: R
authors.lastname: Dewey
authors.forename: L
authors.initials: L
authors.lastname: Semorille
authors.forename: J
authors.initials: J
authors.lastname: Crisci
authors.forename: O
authors.initials: O
authors.lastname: Grau
mesh_terms: D000595:Amino Acid Sequence; D001118:Argentina; D001483:Base Sequence; D003001:Cloning, Molecular; D005814:Genes, Viral; D008969:Molecular Sequence Data; D019251:Nucleocapsid / Q000235:genetics*; D019590:Nucleocapsid Proteins; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid; D017865:Tospovirus / Q000145:classification* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D019590:Nucleocapsid Proteins; D012367:RNA, Viral; C099603:nucleocapsid protein, Tospovirus