title: Stimulated secretion of lysosomal enzymes induced by drugs in transimmortalized proximal tubule mouse kidney cells.
abstract: We summarize the results of study of the properties of two models of transimmortalized proximal tubule epithelial cells derived from the kidneys of transgenic mice harboring the SV40 large T and little t antigens/L-pyruvate kinase hybrid gene. The two cell lines, referred to as PKSV-PCT and PKSV-PR cells, maintained for long-term passages the main biochemical and functional properties from the convoluted and terminal parts of the proximal tubule, respectively, from which they were derived. In PKSV-PCT cells, gentamicin induced lysosomal alkalinization, decreased the cellular N-acetyl-beta-D-glucuronidase, and stimulated its secretion in a dose-dependent manner. The results indicate that these models of mouse proximal cultured cells could be suitable models for the study of the cellular action of drugs.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, INSERM U246, Institut Fédératif de Recherche, Faculté de Médecine Xavier Bichat, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Vandewalle
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004798:Enzymes / Q000187:drug effects / Q000378:metabolism*; D005839:Gentamicins / Q000494:pharmacology*; D005966:Glucuronidase / Q000187:drug effects / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D051379:Mice
chemical_list: D004798:Enzymes; D005839:Gentamicins; D005966:Glucuronidase