title: [O2 sensitive L-lactate biosensors with enzyme membranes based on L-lactate-2-monooxygenase and L-lactate-oxidase with electroanalytic comparison].
abstract: O2-sensitive biosensors using oxidase membranes have acquired considerable electro-analytical importance. Since some of these O2-converting enzymes also produce H2O2, the use of additive reagents for the O2-free breakdown of the H2O2 in the second reaction has repeatedly been reported. In contrast to L-lactate oxidase, L-lactate-2-monooxygenase converts its substrate without producing H2O2. Employing reference sera, tests with L-lactate showed that bioelectrochemical membrane electrodes with H2O2-producing enzymes of high purity, require no additive reagents to ensure reliable analysis. Continuous measurements with citrated blood using the principle of intermediate carrier analysis are demonstrated.
authors.affiliation: ZABS Zentrum für Angewandte Bioelektrochemische Sensorik, Gesellschaft für Forschung, Entwicklung und Produktion mbH, Marburg/Lahn.
authors.forename: H
authors.initials: H
authors.lastname: Guntermann
authors.forename: K
authors.initials: K
authors.lastname: Herna
authors.forename: M M
authors.initials: MM
authors.lastname: Schindler
authors.forename: J G
authors.initials: JG
authors.lastname: Schindler
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis; D004867:Equipment Design; D006801:Humans; D006861:Hydrogen Peroxide; D019344:Lactic Acid / Q000097:blood*; D006899:Mixed Function Oxygenases / Q000097:blood*; D010101:Oxygen Consumption / Q000502:physiology*; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose; D019344:Lactic Acid; D006861:Hydrogen Peroxide; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase