title: Use of immobilized bacteria to treat industrial wastewater containing a chlorinated pyridinol.
abstract: Pseudomonas sp. strain M285 immobilized on diatomaceous earth beads was used to remove 3,5,6-trichloro-2-pyridinol (TCP) from industrial wastewater. Batch studies showed that immobilized Pseudomonas sp. strain M285 mineralized [2,6-14C]TCP rapidly; about 75% of the initial radioactivity was recovered as 14CO2. Transformation of TCP was inhibited by high concentrations of salt, and addition of osmoprotectants (proline and betaine at 1 mM) did not reduce the adverse effect of salt. TCP-containing wastewater (60-140 mg/l) was passed through columns containing immobilized Pseudomonas sp. strain M285 at increasing flow rates and increasing TCP concentrations; TCP removal of 80%-100% was achieved. Addition of nutrients, such as glucose and yeast extract, retarded TCP degradation. Growing cell cultures were found to be better inocula for immobilization than resting cells.
authors.affiliation: Laboratory of Soil Biochemistry, Pennsylvania State University, University Park 16802, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.forename: K D
authors.initials: KD
authors.lastname: Racke
authors.forename: J M
authors.initials: JM
authors.lastname: Bollag
mesh_terms: D001622:Betaine / Q000494:pharmacology; D001673:Biodegradation, Environmental; D019149:Bioreactors; D007220:Industrial Waste; D011392:Proline / Q000494:pharmacology; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D011728:Pyridones / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000295:instrumentation*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D011728:Pyridones; D014874:Water Pollutants, Chemical; D001622:Betaine; C012587:3,5,6-trichloro-2-pyridinol; D011392:Proline