Ovariectomy and estradiol affect postingestive controls of sucrose licking.
Ovariectomy (OVX) has been shown to increase, and estradiol replacement to decrease, meal size in rats. Because little is known about how estradiol influences meals, we conducted two experiments to examine the effects of OVX and beta-estradiol 3-benzoate (EB) replacement on the microstructure of licking behavior. In both experiments, patterns of licking were analyzed in adult female Sprague-Dawley rats during an 0.8 M sucrose test meal. In Experiment 1, meal microstructure was determined preOVX and 10-12 days postOVX. Rate of licking following OVX was not changed during min 1 of the meal, but was significantly faster during min 2-4 of the meal (p < 0.03). The numbers of bursts (runs of licks separated by 250-500 ms) and numbers of clusters (runs of licks separated by > 500 ms) were significantly increased during min 2-4 (p < 0.05). In Experiment 2, OVX rats received EB replacement. Rate of licking after EB replacement was not changed during min 1 of the meal, but was significantly slower during the remainder of the meal (min 2-4, min 5-7, and min 8-10). Burst size, cluster size, and interburst interval were less after EB replacement during min 5-7 of the test meal (all p < 0.05). Because both OVX and EB replacement failed to alter the rate of licking during min 1, estrogen did not appear to alter the palatability of sucrose. OVX and EB replacement did appear to affect a postingestive mechanism(s) that is engaged within 2-4 min of meal onset.
Hrupka
B J
BJ
Department of Psychiatry, Cornell University Medical College, White Plains 10605, USA. hrupka@med.cornell.edu
Smith
G P
GP
Geary
N
N
D000818:Animals; D001066:Appetite / Q000502:physiology; D019422:Dietary Sucrose / Q000008:administration & dosage*; D004958:Estradiol / Q000502:physiology*; D004971:Estrus / Q000502:physiology; D005260:Female; D009042:Motivation; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012528:Satiety Response / Q000502:physiology*; D013649:Taste / Q000502:physiology*
D019422:Dietary Sucrose; D004958:Estradiol