title: Coding sequence of a hyaluronan synthase homologue expressed during expansion of the mouse cumulus-oocyte complex.
abstract: Maturation of mammalian cumulus cell-oocyte complex in the preovulatory follicle involves the deposition of a hyaluronan-rich extracellular matrix by the cumulus cells. In this study, we report the complete coding sequence of a novel mouse hyaluronan synthase homologue expressed in cumulus cell-oocyte complexes induced to expand in vivo. The time frame of mRNA expression of this molecule correlates well with previous biochemical and immunohistochemical findings on the initiation of hyaluronan synthesis in maturing preovulatory follicles. Evolutionary comparison of the vertebrate hyaluronan synthase homologues indicates that there are at least two genes that code for these proteins.
authors.affiliation: Department of Biomedical Engineering, The Cleveland Clinic Foundation, Ohio 44195, USA. fulop@bme.ri.ccf.org
authors.forename: C
authors.initials: C
authors.lastname: Fülöp
authors.forename: A
authors.initials: A
authors.lastname: Salustri
authors.forename: V C
authors.initials: VC
authors.lastname: Hascall
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D005260:Female; D015870:Gene Expression; D014453:Glucuronosyltransferase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016695:Glycosyltransferases; D006801:Humans; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000096:biosynthesis; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000201:enzymology*; D006080:Ovarian Follicle / Q000201:enzymology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014166:Transferases; D029867:Xenopus Proteins
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D029867:Xenopus Proteins; D006820:Hyaluronic Acid; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases