[The role of the transforming growth factor beta 1 (TGF-beta 1) and of vascular endothelial growth factor (VEGF) on the in vitro angiogenesis process].
We have studied the role of 2 exogenous cytokines, the TGF beta 1 and the VEGF, on the in vitro angiogenesis process. Endothelial cells were plated on fibrin matrices either with 2% or with 10% human serum in 199 medium. Forty-eight hours later, with 10% human serum, the capillary-like network index (the percentage of the culture area covered by the capillary network) was about 50%. Under these culture conditions, when TGF-beta 1 or VEGF were added, the capillary-like network index augmented and was nearing 75%. When the index is high, using confocal microscopy, we show that hollow capillaries are formed. Moreover, the addition of VEGF increased the kinetics of the capillary-like network formation. With 2% human serum, 48 h after seeding the capillary-like network index was about 75%. In this case, the addition of TGF-beta 1 decreased the network index, whereas the addition of VEGF increased the kinetics of its formation. These in vitro angiogenesis experiments show that the serological factors underline the 2 antagonist effects of TGF-beta 1, but have no detectable effects on the activator effects on the VEGF.
Vailhé
B
B
Laboratoire de bioénergétique, Université Joseph-Fourier, Grenoble, France.
Tranqui
L
L
D002455:Cell Division / Q000187:drug effects; D016228:Endothelial Growth Factors / Q000737:chemistry*; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D008222:Lymphokines / Q000737:chemistry*; D018613:Microscopy, Confocal; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors