Dose-response of X-irradiated human and equine lymphocytes.
We have investigated and compared DNA damage and cell killing induced in human and equine lymphocytes after in vitro X-irradiation. Our data show that the cytogenetic and the lethality effects are both greater in equine lymphocytes, but that the difference is wider for lethality. The ratios between doses inducing the same effect are 1.3, 1.7 and 9.4 for the number of binucleated cells with micronuclei, micronucleus frequency in binucleated cells and DNA synthesis inhibition, respectively. The very different radiosensitivity observed for the two mammalian species encourages us to use their lymphocytes in cell radiobiology studies.
Catena
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C
Division of Toxicology, ENEA CR Casaccia, Rome, Italy.
Asprea
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L
Carta
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S
Tortora
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G
Conti
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D
Parasacchi
P
P
Righi
E
E
D000818:Animals; D016923:Cell Death / Q000528:radiation effects; D002467:Cell Nucleus / Q000528:radiation effects; D002478:Cells, Cultured; D002875:Chromosomes / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis; D004249:DNA Damage / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006736:Horses; D006801:Humans; D008214:Lymphocytes / Q000000981:diagnostic imaging*; D011859:Radiography
D004247:DNA