title: On the mechanism of the differential effects of NS004 and NS1608 in smooth muscle cells from guinea pig bladder.
abstract: Our recent study revealed that the reported BK(Ca) channel openers NS004 (5-trifluoromethyl-(5-chloro-2-hydroxyphenyl)-1,3-dihydro-2H-benzimidazo le-2-one) and its analog NS1608 (N-(3-(trifluoromethyl)phenyl)-N'-(2-hydroxy-5-chlorophenyl)urea) produced a differential pattern of action on the BK(Ca) channel in porcine coronary arterial cells (Hu, S., H.S. Kim and C.A. Fink, 1995, Differential effects of the BK(Ca) channel openers NS004 and NS1608 in porcine coronary arterial cells, Eur. J. Pharmacol. 294, 357). In this study, using the patch-clamp method on the smooth muscle cells from guinea pig bladder detrusor, the activity profile of NS004 and NS1608 on the whole-cell BK(Ca) current (I(BK)) was examined and found to be similar to that in coronary arterial cells. NS004 did not significantly modify I(BK) at concentrations below 5 microM, but robustly augmented I(BK) at concentrations greater than 20 microM. With a minimum effective concentration of 0.5 microM, NS1608 caused potentiation of I(BK) with a bell-shaped concentration-response relationship. The activation was maximized between 5-10 microM and substantially attenuated at higher concentrations. The behavior of single BK(Ca) channels in the presence of NS004 and NS1608 was scrutinized to elucidate the mechanism underlying their distinct patterns of action. The channel open-state probability (NPo) was increased by NS004 at 0.5, 5 and 50 microM to a respective 1.75 +/- 0.38, 4.05 +/- 0.90, and 15.01 +/- 3.66 fold (n = 7) of the control by increasing the open time and the frequency of opening while having no effect on the single BK(Ca) channel conductance. NS1608 at 0.5 and 5 microM increased NPo to 1.69 +/- 0.43 and 18.03 +/- 6.64 fold of the control (n = 4), respectively. NS1608 at higher concentrations repeatedly blocked channel openings as evidenced by the highly flickering open state, though the overall open time and frequency of opening remained high. The diminished activation of I(BK) by 50 microM NS1608 manifested therefore a net result of these two opposing actions on the single channels. Thus, the two structurally related BK(Ca) channel openers interact distinctly with the channel gating components.
authors.affiliation: Research Department, Pharmaceuticals Division, Ciba-Geigy Corp., Summit, NJ 07901, USA.
authors.forename: S
authors.initials: S
authors.lastname: Hu
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002733:Chlorophenols / Q000494:pharmacology*; D004533:Egtazic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D010671:Phenylurea Compounds / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects* / Q000502:physiology; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology
chemical_list: D001562:Benzimidazoles; D002733:Chlorophenols; D010671:Phenylurea Compounds; D015221:Potassium Channels; C103727:NS 1608; D004533:Egtazic Acid; C083230:NS 004; D002118:Calcium