title: RHD epitope density profiles of RHD variant red cells analyzed by flow cytometry.
abstract: For DII and DIVa, RHD antigen sites per cell were tested previously only with a limited number of radio labelled anti-D. We determined RHD antigen densities (sites/cell) by flow cytometry with 64 anti-D in four partial RHD red cells. Epitope densities detected (mean +/- SD) were: DVII 8,000 +/- 1,665 (number of anti-D used for calculation: n = 55; percentage of reference cells: 47%); DNU (DIIlike) 6,869 +/- 1,381 (n = 47; 29%); and DHMii 20,626 +/- 4,320 (n = 55; 87%). One DIV cell revealed two peaks with a low fluorescence range of 18,158 +/- 2,504 (n = 11; 76%) and a high range of 35,477 +/- 2,485 (n = 6; 149%). We established epitope density profiles with a large panel of anti-D for four RHD variant cells and determined the number of RHD antigens per cell.
authors.affiliation: Abteilung für Transfusionsmedizin, Universität Ulm, Germany.
authors.forename: W A
authors.initials: WA
authors.lastname: Flegel
authors.forename: F F
authors.initials: FF
authors.lastname: Wagner
mesh_terms: D000939:Epitopes / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
chemical_list: D000939:Epitopes; D012204:Rh-Hr Blood-Group System