A monoclonal antibody (1F3) to human glomerular epithelial cells: a new marker for renal epithelial cell injury.
In order to identify a new cell surface antigen as a potential marker of renal epithelial cell injury, we produced a monoclonal antibody (Mab), 1F3, by immunizing mice with cultured human glomerular cells. Immunofluorescence (IF) and immunoelectron microscopy (IEM) studies demonstrated that 1F3-recognizing antigen (1F3 antigen) was strongly expressed on the cell surface of glomerular podocytes and very weakly on parietal epithelial cells. 1F3 antigen was not expressed in any other cells in the normal kidney. Immunoprecipitation analysis using metabolically labeled glomeruli revealed that 1F3 recognized a 125-kD protein under reducing conditions. IF studies of biopsy specimens from patients with a variety of glomerular and tubulointerstitial diseases showed that 1F3 antigen was almost negative in cellular crescents but was strongly expressed in fibrocellular crescents. When glomerular sclerosis appeared, the expression of 1F3 antigen decreased in sclerotic areas of glomeruli. 1F3 antigen became positive in atrophic tubules that were seen in diseased kidneys. Severity of tubular atrophy correlated well with the extent of tubular expression of 1F3 antigen. These results indicate that Mab, 1F3 marks phenotypic changes of renal epithelial cells under disease conditions and may be a useful marker for progressive kidney diseases.
Kagami
S
S
Department of Pediatrics, School of Medicine, University of Tokushima, Japan.
Morimoto
Y
Y
Okada
K
K
Yasutomo
K
K
Kuhara
T
T
Kuroda
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000954:Antigens, Surface / Q000276:immunology*; D015415:Biomarkers; D001706:Biopsy; D004848:Epithelium / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D005921:Glomerulonephritis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D007684:Kidney Tubules / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D010641:Phenotype; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015415:Biomarkers