Early cell kinetic effects of some drugs on the rat corneal and conjunctival epithelia.
The early cell kinetic response in the rat corneal and conjunctival epithelia was studied after a single topical application of dipivefrine, adrenaline and timolol with and without benzalkonium chloride. Benzalkonium chloride alone in different concentrations, 0.004%, 0.01%, 0.02% and 0.04%, respectively, was also evaluated. The stathmokinetic method and the tritiated thymidine technique were used to estimate the mitotic rate and the labelling index. Dipivefrine (Propine) gave a significant depression of the mitotic rate almost to the same extent as adrenaline in the corneal epithelium, whereas no changes were found in the limbal area and in the conjunctiva. Timolol (Oftan) with or without benzalkonium chloride, and benzalkonium chloride in different concentrations gave no obvious changes of the mitotic rate. No distinct drug effects on the labelling index were observed.
Estil
S
S
Department of Ophthalmology, National Hospital, Oslo, Norway.
Haaskjold
E
E
Bjerknes
R
R
Refsum
S B
SB
D000818:Animals; D002453:Cell Cycle; D003228:Conjunctiva / Q000166:cytology / Q000187:drug effects*; D003315:Cornea / Q000166:cytology / Q000187:drug effects*; D004837:Epinephrine / Q000031:analogs & derivatives / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005260:Female; D008938:Mitosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013936:Thymidine
C015173:dipivefrin; D013936:Thymidine; D004837:Epinephrine