title: Determination of antifungals in rodent diet by supercritical fluid extraction followed by packed column supercritical fluid chromatography with ultraviolet detection.
abstract: Supercritical fluid extraction (SFE) followed by packed column supercritical fluid chromatography with ultraviolet detection was evaluated as a quantitative method for determining 4 antifungals (fluconazole, tioconazole, hexaconazole, and UK-47,265) in rodent diet. Chromatography was achieved with a cyano-bonded silica column, UV detection at 210 nm, and methanol-modified supercritical carbon dioxide as mobile phase. The effects of modifier concentration, temperature, and column pressure on antifungal retention time was studied. Off-line SFE was optimized at 2 spike levels, ranging from 0.5 to 10 g/kg, for each of the 4 antifungals. Average recoveries ranged from 79.0% for UK-47,265 to 96.5% for hexaconazole. Overall, the procedure provides a suitable method for analyzing antifungals in spiked rodent diet.
authors.affiliation: University of Northumbria at Newcastle, Department of Chemical and Life Sciences, Newcastle upon Tyne, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Dean
authors.forename: I A
authors.initials: IA
authors.lastname: Fowlis
authors.forename: S M
authors.initials: SM
authors.lastname: Hitchen
authors.forename: S
authors.initials: S
authors.lastname: Khundker
authors.forename: E
authors.initials: E
authors.lastname: Ludkin
authors.forename: F
authors.initials: F
authors.lastname: Normand
authors.forename: P
authors.initials: P
authors.lastname: Jones
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000032:analysis* / Q000378:metabolism; D002853:Chromatography, Liquid; D004032:Diet; D015725:Fluconazole / Q000032:analysis* / Q000378:metabolism; D005504:Food Analysis / Q000592:standards; D005506:Food Contamination; D007093:Imidazoles / Q000032:analysis* / Q000378:metabolism; D012015:Reference Standards; D012377:Rodentia; D013056:Spectrophotometry, Ultraviolet; D014230:Triazoles / Q000032:analysis* / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D007093:Imidazoles; D014230:Triazoles; C057282:dichlorophenyl-bis-triazolylpropanol; D015725:Fluconazole; C020527:tioconazole