title: High-speed DNA sequencing in ultrathin slab gels.
abstract: There have been many recent theoretical and technical advances in the sequencing of DNA in ultrathin slab gels. These include recent experimental progress in four areas: DNA polymerases; DNA template preparation; the separation and detection of DNA bands; and base-calling algorithms. The Zimm-Lumpkin theory of electrophoresis provides a new and improved framework for understanding the operation of sequencing gels.
authors.affiliation: Visible Genetics Inc, 700 Bay Street, Suite 1000, Box 333, Toronto M5G 1Z6, Ontario, Canada.
authors.forename: T D
authors.initials: TD
authors.lastname: Yager
authors.forename: J M
authors.initials: JM
authors.lastname: Dunn
authors.forename: J K
authors.initials: JK
authors.lastname: Stevens
mesh_terms: D000465:Algorithms; D001709:Biotechnology; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018076:DNA, Complementary / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005069:Evaluation Studies as Topic; D015870:Gene Expression; D016678:Genome; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000639:trends
chemical_list: D018076:DNA, Complementary; D004247:DNA; D004259:DNA-Directed DNA Polymerase