Inhibitory effect of chlorophyllin on the frequency of sister chromatid exchanges produced by benzo[a]pyrene in vivo.
The study was designed to determine the antigenotoxic potential of chlorophyllin (Chl), against the frequency of sister-chromatid exchanges (SCE) produced by benzo[a]pyrene (B[a]P) in vivo. We used the mouse bone marrow test system to measure the effect of a single injection of the compounds: 40 mg/kg of B[a]P, and 1 h later, 1.0, 2.0, 4.0 and 8.0 mg/kg of Chl. As controls we included both chemicals using the dosages mentioned above as well as mineral oil (0.25 mg/kg). The results indicated the following: (1) Chl per se was not genotoxic, showing SCE values in the range of the control level; (2) B[a]P increased the rate of SCEs three times in relation to the basal level; (3) the SCE level produced with B[a]P was diminished by all 4 doses of Chl, but better results were obtained with 2-4 mg/kg, a range which induced Inhibition Indices of 80.9% and 77.5% respectively; (4) the Average Generation Time Index was not modified by the compounds used in the experiment; and (5) the Mitotic Index also showed no significant modification induced by the chemicals, with respect to the control value.
Madrigal-Bujaidar
E
E
Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, México D.F., México.
Velázquez-Guadarrama
N
N
Díaz-Barriga
S
S
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology; D001854:Bone Marrow Cells; D002735:Chlorophyllides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D016587:Antimutagenic Agents; D002735:Chlorophyllides; D009153:Mutagens; D001564:Benzo(a)pyrene; C007020:chlorophyllin