Identification of a frameshift mutation resulting in premature termination and loss of cell wall anchoring of the PAc antigen of Streptococcus mutans GS-5.
Most strains of Streptococcus mutans possess a 190-kDa protein antigen (PAc) on their cell surfaces, while strain GS-5 produces extracellularly a 155-kDa PAc protein. The pac gene of strain GS-5 consists of 3,477 bp and codes for a protein of 1,158 amino acids. One insertion of an adenine into the 3,469th, 3,470th, or 3,471st position from the start codon results in a frameshift mutation at codon 1157 with subsequent termination after 3 additional codons.
Murakami
Y
Y
Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka, Japan.
Nakano
Y
Y
Yamashita
Y
Y
Koga
T
T
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D001426:Bacterial Proteins; D001483:Base Sequence; D015153:Blotting, Western; D002473:Cell Wall / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D016368:Frameshift Mutation; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010957:Plasmids; D013295:Streptococcus mutans / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D014169:Transformation, Bacterial
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; C055764:S-layer proteins