title: Evidence that KpsT, the ATP-binding component of an ATP-binding cassette transporter, is exposed to the periplasm and associates with polymer during translocation of the polysialic acid capsule of Escherichia coli K1.
abstract: KpsT utilizes ATP to effect translocation of the polysialic acid capsule of Escherichia coli K1. We have previously proposed a mechanistic model for the action of this protein. Here, we provide evidence to support two predictions of the model: that KpsT associates with polymer and that KpsT is accessible from the periplasmic surface of the inner membrane.
authors.affiliation: Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Bliss
authors.forename: R P
authors.initials: RP
authors.lastname: Silver
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D016667:Bacterial Capsules / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D011233:Precipitin Tests; D012794:Sialic Acids / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012794:Sialic Acids; C021319:polysialic acid; C068103:kpsT protein, E coli; D000255:Adenosine Triphosphate; D014357:Trypsin