Simultaneous restitution of matrix and cells in gastric ulcer: use of a combined in-situ hybridization and immunohistochemistry technique applicable to paraffin-embedded tissue.
The restoration of gastric tissue after ulceration involves cellular and matrix components. Our aim was to investigate the kinetics of collagen expression and cellular proliferation in an animal model of gastric ulcer. To demonstrate the expression of type I and IV collagen mRNAs by proliferating cells, a method combining in-situ hybridization and immunohistochemistry was devised. In order to avoid the disadvantages of radioisotopes, digoxigenin-labeled RNA-riboprobes were utilized and combined with single-step immunohistochemistry. This method proved sensitive enough to detect type I and IV procollagen mRNA transcripts in the submucosal area beneath the ulcer crater or adjacent to the ulcer rim. In addition, a subset of cells transcribing either procollagen type I or IV RNA was concomitantly positive for proliferating cell nuclear antigen by immunohistochemistry. Focal proliferation of cells simultaneously expressing extracellular matrix components may therefore occur in the gastric submucosa after ulceration, starting as soon as 3 days after the insult and continuing for several weeks. The devised method of combined in-situ hybridization and immunohistochemistry can be used with standard paraffin-embedded tissues, yields results within 2 days, and avoids radioisotopes.
Pohle
T
T
Department of Internal Medicine B, University of Münster, Albert-Scheitzer-Str. 33, D-48129, Münster, Germany.
Drees
M
M
Gillessen
A
A
Shahin
M
M
Voss
B
B
Domschke
W
W
D019342:Acetic Acid; D000818:Animals; D003094:Collagen / Q000032:analysis / Q000235:genetics; D004076:Digoxigenin; D005109:Extracellular Matrix / Q000737:chemistry* / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D010232:Paraffin; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology; D016707:Tissue Fixation
D015347:RNA Probes; D012333:RNA, Messenger; D010232:Paraffin; D003094:Collagen; D004076:Digoxigenin; D019342:Acetic Acid