Studies of DEAE-dextran-mediated gene transfer.
DEAE-dextran-mediated gene transfer was studied for the introduction of pSV2neo DNA into Fisher-rat 3T3 (FR3T3) cells. Zeta (zeta) potentials of the DEAE-dextran-DNA complexes and FR3T3 cells were found to be dependent on the concentration of DEAE-dextran in the medium. The maximum transfection efficiency occurred at a DEAE-dextran/DNA ratio of 50:1 or thereabouts. The interaction between DNA and cells is determined by the adsorption process. The results obtained, along with the correlation between the kinetic adsorption behaviour of 3H-labelled DNA and the transfection efficiency, indicated that adsorption of DEAE-dextran-DNA complexes to the negatively charged cell surfaces, due to electrostatic and dispersion attraction, plays the decisive role in determining the DNA transfection efficiencies.
Yang
Y W
YW
School of Pharmacy, College of Medicine, National Taiwan University, Taipei.
Yang
J C
JC
D016475:3T3 Cells; D000818:Animals; D003637:DEAE-Dextran / Q000737:chemistry*; D004274:DNA, Recombinant; D051379:Mice; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection
D004274:DNA, Recombinant; D003637:DEAE-Dextran