title: Alternative splicing of the erythropoietin receptor gene correlates with erythroid differentiation in rat hematopoietic and leukemic cells.
abstract: An alternative splicing of the rat erythropoietin receptor (EpoR) gene was identified in normal and erythroleukemia cells. A 105 bp insert was found at a region corresponding to the extracellular domain of EpoR. The alternative transcript was translated to a soluble EpoR (EpoR-S) expressed in spleen, bone marrow, and cultured erythroleukemia cells in addition to the full-length EpoR (EpoR-F). One of the rat erythroleukemia sublines, K4DT, which partially lost erythroid phenotypes and manifested monocyte/macrophage characteristics also lacked EpoR-S expression. Thus, expression of EpoR-S may play an important role in differentiation of rat erythroid cells.
authors.affiliation: Second Department of Pathology, Kobe University School of Medicine, Kusunoki-cho, Chuo-ku, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fujita
authors.forename: R
authors.initials: R
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Kitada
authors.forename: R
authors.initials: R
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Kitazawa
authors.forename: F
authors.initials: F
authors.lastname: Ashoori
authors.forename: P
authors.initials: P
authors.lastname: Liang
authors.forename: H
authors.initials: H
authors.lastname: Saya
authors.forename: T
authors.initials: T
authors.lastname: Serikawa
authors.forename: S
authors.initials: S
authors.lastname: Maeda
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D015672:Erythroid Precursor Cells / Q000166:cytology*; D005914:Globins / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017467:Receptors, Erythropoietin / Q000235:genetics*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D012333:RNA, Messenger; D017467:Receptors, Erythropoietin; D005914:Globins