High-performance liquid chromatographic determination of N-alpha-acetyl-L-carnosine in equine plasma.
N-alpha-Acetyl-L-carnosine (NAcCAR) in perchloric acid extracts of equine plasma was assayed by high-performance liquid chromatography on a 3 microns Hypersil ODS (150 x 4.6 mm I.D.) column eluted with 5 mM phosphoric acid-1 mM triethylamine, pH 2.58. NAcCAR was isolated by solid-phase extraction on Isolute PRS (propylsulphonyl) columns. The HPLC mean retention time for NAcCAR was 5.9 +/- 0.2 min. The recovery from plasma by solid-phase extraction was 93.9-99.7% and lower limit of detection in plasma was 0.18 microM. The normal NAcCAR concentration in equine plasma was 2.4 +/- 0.3 microM. The method was applied to the determination of plasma concentrations following oral and intravenous NAcCAR administration.
Dunnett
M
M
Equine Sports Medicine Unit, Royal Veterinary College, North Mymms, Hertfordshire, UK.
D000818:Animals; D002336:Carnosine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D006639:Histidine / Q000097:blood / Q000378:metabolism; D006736:Horses / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D006639:Histidine; D002336:Carnosine