title: Multichannel microchip electrospray mass spectrometry.
abstract: Microfabricated multiple-channel glass chips were successfully interfaced to an electrospray ionization mass spectrometer (ESI-MS). The microchip device was fabricated by standard photolithographic, wet chemical etching, and thermal bonding procedures. A high voltage was applied individually from each buffer reservoir for spraying sample sequentially from each channel. With the sampling orifice of the MS grounded, it was found that a liquid flow of 100-200 nL/min was necessary to maintain a stable electrospray. The detection limit of the microchip MS experiment for myoglobin was found to be lower than 6 x 10(-8) M. Samples in 75% methanol were successfully analyzed with good sensitivity, as were aqueous samples. The parallel mutliple-channel microchip system allowed ESI-MS analysis of different samples of standard peptides and proteins in one chip.
authors.affiliation: Barnett Institute, Northeastern University, Boston, Massachusetts 02115, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Xue
authors.forename: F
authors.initials: F
authors.lastname: Foret
authors.forename: Y M
authors.initials: YM
authors.lastname: Dunayevskiy
authors.forename: P M
authors.initials: PM
authors.lastname: Zavracky
authors.forename: N E
authors.initials: NE
authors.lastname: McGruer
authors.forename: B L
authors.initials: BL
authors.lastname: Karger
mesh_terms: D013058:Mass Spectrometry / Q000295:instrumentation*; D008838:Microcomputers; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis
chemical_list: D010455:Peptides; D011506:Proteins