title: Production and characterization of a new monoclonal antibody effective in recognizing the CD3 T-cell associated antigen in formalin-fixed embedded tissue.
abstract: Phenotypic analysis of lymphoproliferative disorders is now considered mandatory for accurate classification which is the basis for optimum patient management. This is presently carried out in most cases using a range of antibodies recognizing B and T-cell antigens effective in paraffin sections, and an antibody to CD 3 is currently a key member of such panels, indicating T-cell phenotype. Current antibodies to CD3 are polyclonal with the inherent disadvantages of this type of reagent compared to monoclonal antibodies. In this study, we have used a recombinant fusion protein representing part of the epsilon subunit of the CD3 molecule to generate a novel monoclonal antibody (NCL-CD3-PS1) effective in paraffin sections. The antibody has been characterized biochemically and by immunohistochemistry using a wide range of normal and pathological tissues. Lineage and phenotype specificity have been supported in our study and results from other laboratories are awaited with interest.
authors.affiliation: University Department of Pathology, Royal Victoria Infirmary, University of Newcastle upon Tyne, UK.
authors.forename: M
authors.initials: M
authors.lastname: Steward
authors.forename: R
authors.initials: R
authors.lastname: Bishop
authors.forename: N H
authors.initials: NH
authors.lastname: Piggott
authors.forename: I D
authors.initials: ID
authors.lastname: Milton
authors.forename: B
authors.initials: B
authors.lastname: Angus
authors.forename: C H
authors.initials: CH
authors.lastname: Horne
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry*; D017252:CD3 Complex / Q000032:analysis / Q000276:immunology*; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000473:pathology; D016612:Paraffin Embedding; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry*; D016707:Tissue Fixation
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D011994:Recombinant Proteins; D005557:Formaldehyde