Glutamate dehydrogenase antigen detection in Plasmodium falciparum infections.
The usefulness of malaria diagnosis by Plasmodium falciparum GDH (NADP+), obtained by affinity chromatography, is demonstrated in ELISA assays, testing IgG antibodies against GDH (NADP+) from patients with acute malaria, who have had two or more episodes of malaria, or from sera of hyperimmune patients. GDH (NADP+) thermal stability was demonstrated in a high heat resistance assay. The immunofluorescence assay demonstrated that anti-culture (P. falciparum) supernatant serum and anti-GDH (NADP+) of Proteus spp. recognized epitopes in Venezuelan isolates, and Colombian and Brasilian malarial strains. The antigen is soluble, with high specificity, is a potent immunogen and is thermoresistant.
de Dominguez
N
N
Universidad Central de Venezuela Tropical Medicine Institute Immunochemistry Department, Caracas, Venezuela.
Rodriguez-Acosta
A
A
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay; D005969:Glutamate Dehydrogenase / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000469:parasitology; D051379:Mice; D010963:Plasmodium falciparum / Q000201:enzymology*; D012995:Solubility
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D005969:Glutamate Dehydrogenase