Detection of different metabolites in the rabbit lens by high resolution 1H NMR spectroscopy.
OBJECTIVE
To investigate the metabolic profile of the rabbit lens using high resolution 1H NMR spectroscopy including two-dimensional shift correlated (COSY) technique.


METHODS
Perchloric acid extracts of the rabbit lens were analysed with a Bruker AM-500 spectrometer and the metabolites were assigned in the spectra. Some of these were also quantified.


RESULTS
More than 20 metabolites were detected in the perchloric acid extract of a single lens, including amino acids, nucleotides and other related compounds. Of particular importance is the ability to detect and identify glutathione, myoinositol, scyllo-inositol and taurine.


CONCLUSIONS
The study demonstrated the potential of 1H NMR spectroscopy for monitoring the metabolic profile of the lens in normal and pathologic conditions.
Midelfart
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A
Department of Ophthalmology, Faculty of Medicine, Norwegian Institute of Technology, University of Trondheim, Norway.
Dybdahl
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A
Gribbestad
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S
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002682:Chinchilla; D007908:Lens, Crystalline / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009711:Nucleotides / Q000032:analysis*; D011817:Rabbits; D012680:Sensitivity and Specificity
D000596:Amino Acids; D009711:Nucleotides