Nonrandom gain of chromosome 7 in central neurocytoma: a chromosomal analysis and fluorescence in situ hybridization study.
Central neurocytoma is a benign, slow-growing neoplasm with favourable prognosis. Biomolecular analysis has failed to demonstrate significant alterations, and no cytogenetic alterations have been reported. In this study we demonstrate chromosome 7 gain in three of nine neurocytomas (33%). Traditional cytogenetic analysis performed in four of the nine cases identified trisomy 7 as the sole chromosomal abnormality in one case. Interphase cytogenetics utilizing fluorescent in situ hybridization (FISH) on cell suspensions from formalin-fixed paraffin-embedded tumour tissue performed in all nine cases detected trisomy 7 in two more cases and tetrasomy in another. Our results suggest that chromosome 7 gain is a feature of neuroectodermal tumorigenesis, possibly conferring growth advantage on the neoplastic cells. FISH on interphase nuclei is a valuable adjunct in the genetic evaluation of rare central nervous system neoplasms with low baseline proliferative activity.
Taruscio
D
D
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
Danesi
R
R
Montaldi
A
A
Cerasoli
S
S
Cenacchi
G
G
Giangaspero
F
F
D000328:Adult; D002551:Cerebral Ventricle Neoplasms / Q000235:genetics; D002869:Chromosome Aberrations / Q000235:genetics*; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D003582:Cytogenetics / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D018306:Neurocytoma / Q000235:genetics*; D014314:Trisomy / Q000235:genetics*