Chromogenic lactate-leukocyte esterase substrates.
The first successful reported use of lactate-based chromogenic, colorimetric substrates for a serine protease-based enzyme is described. A series of hydroxy-protected 5-phenyl-3-hydroxypyrrolyl L-lactate substrates of the general formula RO-Lac-OPP were prepared and formulated into reagents for the determination of leukocytes in dry phase formats.
Johnson
G M
GM
Diagnostics Division, Bayer Corporation, Elkhart, Indiana 46515, USA.
Schaeper
R
R
D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002863:Chromogenic Compounds / Q000138:chemical synthesis*; D003124:Colorimetry / Q000379:methods; D006868:Hydrolysis; D019344:Lactic Acid / Q000378:metabolism*; D011933:Reagent Kits, Diagnostic
D002863:Chromogenic Compounds; D011933:Reagent Kits, Diagnostic; D019344:Lactic Acid; C021443:leukocyte esterase; D002265:Carboxylic Ester Hydrolases