title: Glucosyltransferase activity of commercial juice-processing enzyme preparations.
abstract: Of various commercial enzyme preparations examined, Cytolase M102 was found to contain the highest glucosyltransferase activity (55 U ml-1). It rapidly converted maltose to panose (Glc alpha-->6 Glc alpha l-->4 Glc) with a Vmax value of 5.8 mmol l-1 min-1 at 50 degrees C in 0.05 mol l-1 sodium acetate buffer (pH 4.4). The Km value of the enzyme for maltose was 750 mmol l-1. Yields of panose and glucose after 45 min of reaction, for example, were 47.2% and 52.8%, respectively, on the basis of the amount of maltose consumed.
authors.affiliation: Department of Food Science and Technology, Cornell University, Geneva, New York, USA.
authors.forename: Y D
authors.initials: YD
authors.lastname: Hang
authors.forename: E E
authors.initials: EE
authors.lastname: Woodams
mesh_terms: D004798:Enzymes / Q000032:analysis* / Q000378:metabolism; D005511:Food Handling; D005516:Food Microbiology; D005638:Fruit / Q000378:metabolism; D005658:Fungi / Q000201:enzymology*; D005936:Glucans / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008320:Maltose / Q000378:metabolism; D015394:Molecular Structure; D013997:Time Factors
chemical_list: D004798:Enzymes; D005936:Glucans; C008763:panose; D008320:Maltose; D005964:Glucosyltransferases; D005947:Glucose