Micellar electrokinetic chromatography as a fast screening method for the determination of the doping agents furosemide and piretanide in urine.
The possibility of using micellar electrokinetic chromatography for the screening of the loop diuretics piretanide and furosemide in urine was studied. A fast and simple method with good repeatability is described. The method was applied to urine samples collected from a healthy volunteer after oral administration of therapeutic doses of each compound. Positive identification in the urine matrix was possible through recording diode array spectra.
Lalljie
S P
SP
Department of Organic Chemistry, University of Ghent, Belgium.
Barroso
M B
MB
Steenackers
D
D
Alonso
R M
RM
Jiménez
R M
RM
Sandra
P
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D004232:Diuretics / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D004300:Doping in Sports; D004563:Electrochemistry; D005665:Furosemide / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D006801:Humans; D008823:Micelles; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000652:urine*
D004232:Diuretics; D008823:Micelles; D013449:Sulfonamides; D005665:Furosemide; C015451:piretanide