title: Phenotypic characterisation of intestinal lymphocytes in ovine paratuberculosis by immunohistochemistry.
abstract: Characterisation of the T-cell subsets in intestinal lesions in sheep with paratuberculosis may contribute to our understanding of the pathogenesis of this disease. To determine the phenotype and distribution of lymphocytes in the normal sheep intestinal mucosa and in Mycobacterium avium subspecies paratuberculosis infected sheep, immunohistochemistry was performed on 12 normal sheep and 18 naturally infected, clinically diseased sheep of which 12 showed lepromatous and six tuberculoid forms of the disease. Immunoperoxidase staining was carried out on frozen sections of ileum using monoclonal antibodies against ovine CD4, CD8, and gamma delta T-cell receptor (TCR) markers. In all three sample groups, cells appeared to be non-randomly distributed throughout the lamina propria. Higher densities of lymphocytes were present in villus than in crypt areas. CD8+ cells were located principally around the epithelial basement membrane, whereas CD4+ cells were localised towards the central villus area of the lamina propria. Lymphocytes bearing the gamma delta T-cell receptor were more widely distributed, both in epithelial and lamina propria compartments. Ileum with tuberculoid lesions had higher densities of CD4 and gamma delta T-cell subsets while lepromatous lesions had lower densities of CD4 and CD8 cells compared with normal tissues. The median relative percentage of CD4+ cells was increased and that of CD8+ cells decreased in tuberculoid cases, with a corresponding increase in the CD4:CD8 ratio, while the relative percentage of gamma delta + cells was increased in lepromatous cases.
authors.affiliation: Department of Veterinary Pathology, University of Edinburgh, Midlothian, UK. David.Little@ed.ac.uk
authors.forename: D
authors.initials: D
authors.lastname: Little
authors.forename: H M
authors.initials: HM
authors.lastname: Alzuherri
authors.forename: C J
authors.initials: CJ
authors.lastname: Clarke
mesh_terms: D000818:Animals; D016516:CD4-CD8 Ratio / Q000662:veterinary; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D007150:Immunohistochemistry; D016130:Immunophenotyping / Q000662:veterinary; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology; D018655:Lymphocyte Count / Q000662:veterinary; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology; D009928:Organ Specificity / Q000276:immunology; D010283:Paratuberculosis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D012756:Sheep; D016176:T-Lymphocyte Subsets / Q000145:classification* / Q000276:immunology