title: The structure, function and distribution of the mouse TWIK-1 K+ channel.
abstract: The two P domain K+ channel mTWIK-1 has been cloned from mouse brain. In Xenopus oocytes, mTWIK-1 currents are K+-selective, instantaneous, and weakly inward rectifying. These currents are blocked by Ba2+ and quinine, decreased by protein kinase C and increased by internal acidification. The apparent molecular weight of mTWIK-1 in brain is 81 kDa. A 40 kDa form is revealed after treatment with a reducing agent, strongly suggesting that native mTWIK-1 subunits dimerize via a disulfide bridge. TWIK-1 mRNA is expressed abundantly in brain and at lower levels in lung, kidney, and skeletal muscle. In situ hybridization shows that mTWIK-1 expression is restricted to a few brain regions, with the highest levels in cerebellar granule cells, brainstem, hippocampus and cerebral cortex.
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, 660, Valbonne, France.
authors.forename: F
authors.initials: F
authors.lastname: Lesage
authors.forename: I
authors.initials: I
authors.lastname: Lauritzen
authors.forename: F
authors.initials: F
authors.lastname: Duprat
authors.forename: R
authors.initials: R
authors.lastname: Reyes
authors.forename: M
authors.initials: M
authors.lastname: Fink
authors.forename: C
authors.initials: C
authors.lastname: Heurteaux
authors.forename: M
authors.initials: M
authors.lastname: Lazdunski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001464:Barium / Q000494:pharmacology; D001483:Base Sequence; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D019281:Dimerization; D017403:In Situ Hybridization; D008564:Membrane Potentials; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009865:Oocytes; D015221:Potassium Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D024683:Potassium Channels, Tandem Pore Domain; D011803:Quinine / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014981:Xenopus
chemical_list: D018076:DNA, Complementary; C486372:Kcnk1 protein, mouse; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D012333:RNA, Messenger; D001464:Barium; D011803:Quinine