Analysis of HLA-DRB1 alleles using PCR-RFLP and PCR-MPH.
In this study we compare the results of HLA-DRB1 genotyping by PCR-RFLP and PCR-MPH. HLA-DR specificities were also performed by LCT. Samples were obtained from 20 Thai patients who were on the waiting list for kidney transplant. DNA was extracted by phenol-chloroform extraction. It was found that the results gave complete agreement with two methods of DNA typing, however, there were 3 discrepancies in assigning serologic DR specificities and DNA subtypes (p = 0.0001) which were due to the cross reactive antibodies and the lack of potent antisera to define proper HLA-DR subtypes by LCT. These PCR techniques can be applied to identify other alleles such as HLA-DPB1 and HLA-DQB1 which will improve the standard histocompatibility testing in the future.
Thalang
O N
ON
Department of Pathology, Pramongkutklao College of Medicine, Bangkok, Thailand.
Sukha
C
C
Suwanasophon
C
C
Maneemaroj
R
R
Okubo
Y
Y
Tatsumi
N
N
D000483:Alleles; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013785:Thailand
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains