The 72nd codon change of p53 in primary renal cell carcinoma was confirmed as a polymorphism among Japanese.
OBJECTIVE
The role of p53 mutation in renal cell carcinoma (RCC) is not fully understood particularly among Japanese, although frequent loss of heterozygosity (LOH) has been demonstrated at polymorphic exon 4.


METHODS
We examined the p53 gene in 43 primary RCC samples by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis for exons 4-9 and restriction fragment length polymorphism (RFLP) analysis.


RESULTS
In PCR-SSCP analysis, no apparent mobility shift was observed regardless of the clinical stage of the disease, histological subtype or malignancy grade of the tumor. In RFLP analysis, none of 13 informative cases showed gross alteration of the gene.


CONCLUSIONS
We conclude that mutations of the p53 gene are not major events in the development and progression of RCC among Japanese.
Kageyama
Y
Y
Department of Urology, School of Medicine, Tokyo Medical and Dental University, Japan.
Yamamura
Y
Y
Oshima
H
H
Ikawa
Y
Y
D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D003062:Codon / Q000235:genetics; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational
D003062:Codon