title: Protection of cattle against Fasciola hepatica infection by vaccination with glutathione S-transferase.
abstract: Glutathione S-transferase (GST) from the liver fluke Fasciola hepatica was assessed as a vaccine immunogen in cattle in a number of immunological adjuvants. Significant reductions in fluke burdens (49-69%) were only observed in cattle vaccinated with GST in Quil Alsqualene Montanide (SM) and PLG microspheres in SM but there was no correlation between anti-GST IgG titres and protection. In separate experiments, animals vaccinated with GST in Quil AlSM were still significantly protected (48%, P < 0.05) 6 months after boosting and no significant differences in protection were seen when the metacercarial challenge was given over 1 month instead of as a single bolus. Inhibition of GST enzyme activity in vitro by cattle antisera did not correlate with reduced fluke burdens.
authors.affiliation: Ciba Animal Health Research, Centre de Recherches Agricoles, St. Aubin, Switzerland.
authors.forename: C A
authors.initials: CA
authors.lastname: Morrison
authors.forename: T
authors.initials: T
authors.lastname: Colin
authors.forename: J L
authors.initials: JL
authors.lastname: Sexton
authors.forename: F
authors.initials: F
authors.lastname: Bowen
authors.forename: J
authors.initials: J
authors.lastname: Wicker
authors.forename: T
authors.initials: T
authors.lastname: Friedel
authors.forename: T W
authors.initials: TW
authors.lastname: Spithill
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000906:Antibodies / Q000097:blood; D001822:Body Burden; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005210:Fasciola hepatica; D005211:Fascioliasis / Q000517:prevention & control / Q000662:veterinary*; D005982:Glutathione Transferase / Q000032:analysis / Q000037:antagonists & inhibitors / Q000276:immunology*; D008297:Male; D062605:Quillaja Saponins; D012503:Saponins; D017186:Titrimetry; D014200:Trematoda; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D062605:Quillaja Saponins; D012503:Saponins; C046386:Quil A; D005982:Glutathione Transferase