The carotenoids beta-carotene, canthaxanthin and zeaxanthin inhibit macrophage-mediated LDL oxidation.
Human monocyte-macrophages were incubated for 24 h in Ham's F-10 medium with human low-density lipoprotein (LDL) in the presence or absence of beta-carotene, canthaxanthin or zeaxanthin, at final concentrations of 2.5, 12.5 and 25 mg/l. LDL oxidation, measured by agarose gel electrophoresis, the thiobarbituric acid assay and gas chromatography, was inhibited by each of the carotenoids in a concentration-dependent manner. Canthaxanthin was more effective when incorporated into LDL before addition to the cultures whereas beta-carotene and zeaxanthin were more effective when added simultaneously with LDL. The results suggest that dietary carotenoids might help slow atherosclerosis progression.
Carpenter
K L
KL
Department of Pathology, University of Cambridge, UK. klhc@mole.bio.cam.ac.uk
van der Veen
C
C
Hird
R
R
Dennis
I F
IF
Ding
T
T
Mitchinson
M J
MJ
D000975:Antioxidants / Q000494:pharmacology*; D016644:Canthaxanthin / Q000494:pharmacology*; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008077:Lipoproteins, LDL / Q000187:drug effects* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000975:Antioxidants; D008077:Lipoproteins, LDL; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D016644:Canthaxanthin