Cloning PCR products utilizing the T/A overhang and a kit.
Lail-Trecker
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Department of Anatomy, University of Connecticut Health Center, Farmington, USA.
D000818:Animals; D001483:Base Sequence; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry; D017931:DNA Primers; D004251:DNA Transposable Elements; D018076:DNA, Complementary / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase; D007202:Indicators and Reagents; D016254:Mutagenesis, Insertional; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000096:biosynthesis; D019914:Taq Polymerase
D015820:Cadherins; D017931:DNA Primers; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D004247:DNA; C072146:Pfu DNA polymerase; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase