Vasoactive intestinal peptide modulates T lymphocyte migration in Peyer's patches of rat small intestine.
Although vasoactive intestinal peptide (VIP) has been postulated to function in modulation of T cell trafficking, the exact mechanism has not been elucidated in vivo. In the present study, the effects of VIP on T lymphocyte migration were examined in rat Peyer's patches. T lymphocytes collected from intestinal lymph of rats were labeled with carboxyfluorescein diacetate succinimidyl ester and injected into the jugular vein. Peyer's patches of the recipient rats were observed with intravital fluorescence microscopy. In vivo intra-arterial infusion of or in vitro incubation with VIP did not affect the initial lymphocyte interaction with postcapillary venules of Peyer's patches. However, these treatments with VIP significantly inhibited transendothelial migration and also significantly blocked the interstitial migration of T cells and inhibited their subsequent appearance in the interfollicular lymphatics. Treatment with adenosine 3',5'-cyclic monophosphate (cAMP)-inducing agents resulted in similar inhibitory effect on T lymphocyte migration in Peyer's patches. In conclusion, VIP has significant inhibitory effects on T lymphocyte migration in Peyer's patches, possibly mediated by elevation of the intracellular cAMP concentrations.
Miura
S
S
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Serizawa
H
H
Tsuzuki
Y
Y
Kurose
I
I
Suematsu
M
M
Higuchi
H
H
Shigematsu
T
T
Hokari
R
R
Hirokawa
M
M
Kimura
H
H
Ishii
H
H
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects*; D008196:Lymph / Q000502:physiology; D008297:Male; D010581:Peyer's Patches / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D015815:Cell Adhesion Molecules; D014660:Vasoactive Intestinal Peptide; D003994:Bucladesine; D000242:Cyclic AMP