title: 13C-NMR study of glucose and pyruvate catabolism in four acetogenic species isolated from the human colon.
abstract: Glucose fermentation by four acetogenic species (two Clostridium strains, one Streptococcus strain and Ruminococcus hydrogenotrophicus) isolated from the human colon was of a mixed-acid type, whereas pyruvate metabolism was characterised by homoacetogenesis. Acetate formation from [1-13C] and [2-13C]glucose was consistent with the formation of acetyl-SCoA from pyruvate generated by the Embden-Meyerhof-Parnas pathway. Labelling of lactate and ethanol demonstrated that these metabolites were formed by reduction of pyruvate and acetyl-SCoA, respectively. In contrast, the reductive pathway of acetate formation was the preferential means of re-oxidising cofactors formed during [1-13C]pyruvate catabolism.
authors.affiliation: Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Jouy en Josas, France.
authors.forename: M
authors.initials: M
authors.lastname: Leclerc
authors.forename: A
authors.initials: A
authors.lastname: Bernalier
authors.forename: M
authors.initials: M
authors.lastname: Lelait
authors.forename: J P
authors.initials: JP
authors.lastname: Grivet
mesh_terms: D000085:Acetates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D003013:Clostridium / Q000302:isolation & purification / Q000378:metabolism; D003106:Colon / Q000382:microbiology*; D004734:Energy Metabolism / Q000502:physiology; D005285:Fermentation / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D019289:Pyruvic Acid / Q000378:metabolism*; D013291:Streptococcus / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000085:Acetates; D002247:Carbon Isotopes; D002245:Carbon Dioxide; D019289:Pyruvic Acid; D005947:Glucose