BofC encodes a putative forespore regulator of the Bacillus subtilis sigma K checkpoint.
A mutation, bofC1, that restores sigma K activation in Bacillus subtilis strains unable to produce active sigma G has been identified. This mutation defines a new sporulation gene, bofC, that has been cloned and sequenced and encodes a 19 kDa protein. bofC is transcribed in the forespore by RNA polymerase associated with the transcription factors sigma F (E sigma F) and sigma G (E sigma G). BofC acts negatively on SpoIVB and the results described suggest that BofC regulates SpoIVB activity and its intercompartmental signalling role in the sigma K checkpoint.
Gomez
Manuel
M
Department of Microbiology, University of Pennsylvania School of Medicine,Philadelphia, PA 19104-6076,USA.
Cutting
Simon M
SM
Department of Microbiology, University of Pennsylvania School of Medicine,Philadelphia, PA 19104-6076,USA.
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002451:Cell Compartmentation; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005796:Genes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C104572:BofC protein, Bacillus subtilis; D008565:Membrane Proteins; D014157:Transcription Factors; C084190:sigma K; C088067:spoIV protein, Bacillus megaterium