title: Rapid molecular haplotyping of the first exon of the human dopamine D4 receptor gene by heteroduplex analysis.
abstract: The dopamine D4 receptor gene (DRD4) and its products are of great interest in many neurospsychiatric disorders. There are at least five expressed polymorphisms in exons 1 and 3, plus rare expressed variants, all of which may have functional relevance. Several studies have described methods for studying the exon 3 polymorphisms, especially the VNTR; fewer reports have documented the exon 1 polymorphisms and variants of DRD4. We report here a simple, rapid, nonisotopic, nondenaturing heteroduplex method for determining the molecular haplotype composed of the two more polymorphic systems of the first exon of DRD4: the 12 bp duplication and 13 bp deletion. This method will facilitate future research on expressed variation of this gene.
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors.forename: F M
authors.initials: FM
authors.lastname: Chang
authors.forename: K K
authors.initials: KK
authors.lastname: Kidd
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons / Q000235:genetics; D005838:Genotype; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D050638:Receptors, Dopamine D4; D017384:Sequence Deletion / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: C493599:DRD4 protein, human; D009692:Nucleic Acid Heteroduplexes; D017448:Receptors, Dopamine D2; D050638:Receptors, Dopamine D4