title: Hepatic allograft procurement from non-heart-beating donors: limits of warm ischemia in porcine liver transplantation.
abstract: To investigate the tolerance to warm ischemia of liver grafts from non-heart-beating donors, porcine orthotopic liver transplantation was performed using grafts obtained at various periods after cardiac arrest. Graft viability was investigated in relation to changes in hepatic adenine nucleotide metabolism. In donors, livers were divided into four groups according to warm ischemic time after cardiac arrest (group 1: 0 min, n=3; group 2: 30 min, n=3; group 3: 60 min, n=5; group 4: 90 min, n=4). Thereafter, the livers were flushed and preserved for 4 hr using 4 degrees C Euro-Collins solution. After surgery, all of the recipients in groups 1, 2, and 3 survived more than 4 days, except for one pig in group 3 that died of bleeding from an arterial catheter on day 2. By contrast, all of the recipients in group 4 died within 12 hr. The serum glutamic oxaloacetic transaminase concentration at 4 hr after reperfusion of the graft was significantly higher in group 4 (mean+/-SE, 2563+/-556 IU/L) than in groups 1, 2, and 3 (298+/-29 IU/L, 1226+/-222 IU/L, and 1181+/-174 IU/L, respectively). The adenylate energy charge of the liver graft recovered at 1 hr after reperfusion of the graft to 0.852+/-0.013, 0.845+/-0.003, and 0.842+/-0.003 in groups 1, 2, and 3, respectively. The recovery was significantly suppressed in group 4 (0.796+/-0.011). The hepatic adenosine triphosphate concentration also was significantly lower in group 4 compared with the other groups. The present study suggests that liver allografts can be used from non-heart-beating donors subjected to warm ischemia for less than 60 min. Postoperative survival is associated with prompt recovery of the adenylate energy charge of the liver graft.
authors.affiliation: Department of Surgery, Institute of Clinical Medicine, Tsukuba University, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takada
authors.forename: H
authors.initials: H
authors.lastname: Taniguchi
authors.forename: K
authors.initials: K
authors.lastname: Fukunaga
authors.forename: K
authors.initials: K
authors.lastname: Yuzawa
authors.forename: M
authors.initials: M
authors.lastname: Otsuka
authors.forename: T
authors.initials: T
authors.lastname: Todoroki
authors.forename: T
authors.initials: T
authors.lastname: Iijima
authors.forename: K
authors.initials: K
authors.lastname: Fukao
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004734:Energy Metabolism; D006323:Heart Arrest; D006358:Hot Temperature; D007511:Ischemia / Q000517:prevention & control*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism; D016031:Liver Transplantation / Q000401:mortality / Q000473:pathology; D016019:Survival Analysis; D013552:Swine; D014019:Tissue Donors; D009927:Tissue and Organ Procurement; D014184:Transplantation, Homologous
chemical_list: D000227:Adenine Nucleotides; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases