title: Preferential potentiation by hypotonic cell swelling of muscarinic cation current in guinea pig ileum.
abstract: The effects of hypotonic cell swelling (HCS) on muscarinic receptor-activated cationic current in guinea pig ileal smooth muscle were investigated by the whole cell patch-clamp technique. With nystatin-perforated recording, reduced external tonicity from 312 to 262 mosM caused cell swelling but hardly affected the membrane currents activated by depolarization, such as outward-rectifying K and voltage-dependent Ca currents. In contrast, the inward current evoked by carbachol at -60 mV was greatly increased (approximately 50%) by the same extent of hypotonicity. This effect is likely to occur through potentiation of nonselective cation channels coupled to the muscarinic receptor (mNSCCs) and probably does not involve elevated intracellular Ca2+ concentration ([Ca2+]i), since neither removal of external Ca2+ nor [Ca2+]i buffering with 10 mM 1,2-bis-(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid significantly affected the results. Furthermore, the time course and degree of this potentiation closely matched those of video-microscopically monitored HCS. These results support the view that mechanosensitive modulation may be a powerful mechanism to regulate mNSCCs activity in gut smooth muscle, together with membrane potential and [Ca2+]i.
authors.affiliation: Second Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Waniishi
authors.forename: R
authors.initials: R
authors.lastname: Inoue
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002412:Cations / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004553:Electric Conductivity; D004594:Electrophysiology; D005260:Female; D006168:Guinea Pigs; D007038:Hypotonic Solutions / Q000494:pharmacology*; D007082:Ileum / Q000166:cytology / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology*; D008297:Male; D009116:Muscarine / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011976:Receptors, Muscarinic / Q000502:physiology; D013997:Time Factors
chemical_list: D002412:Cations; D007038:Hypotonic Solutions; D007473:Ion Channels; D011976:Receptors, Muscarinic; D009116:Muscarine; D002217:Carbachol