[Isolation and physico-chemical characteristics of Staphylococcus hyaluronidase].
A scheme for purification of hyaluronidase from Staphylococcus aureus 0-15 that allows to produce a 550-fold-purified preparation with a high specific activity was developed. Hyaluronidase was found to consist of two molecular forms with different molecular weights. Staphylococcus hyaluronidase referred to as a complex preparation and its molecular forms have action optimum at pH 5.0-7.2 and at a temperature between 30 and 37 degrees C. The enzymes were highly specific to their substrate: they catalyzed the depolimerization of hyaluronic acid but did not split the other glycosaminoglycans.
Turmanidze
T S
TS
Kvesitadze
G I
GI
Mikanadze
I U
IU
Shanidze
M G
MG
Loladze
M Z
MZ
Birkadze
T V
TV
Alibegashvili
M G
MG
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000302:isolation & purification*; D013210:Staphylococcus / Q000201:enzymology*
D001426:Bacterial Proteins; D006821:Hyaluronoglucosaminidase