title: Establishment and characterization of human gastric carcinoma cell lines.
abstract: We report 8 newly established gastric-carcinoma cell lines (SNU-216, 484, 520, 601, 620, 638, 668, 719) from Korean patients. Morphologic study was carried out using light and electron microscopes. CEA, alpha FP, and CA 19-9 and TPA in supernatant and in cell lysate were measured by radioimmunoassay. p53 and c-Ki-ras gene mutations were screened and confirmed by sequencing. The cell lines, derived from tumors with moderate differentiation, grew as a diffuse monolayer, and those from tumors with poor differentiation and minimal desmoplasia grew exclusively as non-adherent. Out of the 8 gastric-cancer cell lines, 5 had detectable levels of CEA both in supernatant and in cell lysate; there was no expression or secretion of alpha FP in these cells; 4 cell lines showed high levels of CA 19-9 in cell pellets. All cell lines except SNU-484 had high concentrations of TPA both in cell lysate and in supernatants. p53 mutation was found in 6 cell lines (75%): 2 (SNU-216 and SNU-668) had mutations in exon 6, and other 3 in exon 8. The c-Ki-ras mutation was found in 2 cell lines (25%), SNU-601 and SNU-668. The former showed GGT-to-GAT transition mutation at codon 12, while the latter showed CAA-to-AAA transversion mutation at codon 61. DNA profiles using restriction endonuclease HinfI and polymorphic DNA probes ChdTC-15 and ChdTC-114 showed different unique patterns; which suggests that these cell lines are unique and not cross-contaminated. We believe that the newly characterized gastric-cancer cell lines presented in this paper will provide a useful in vitro model for studies related to human gastric cancer.
authors.affiliation: Laboratory of Cell Biology, Cancer Research Institute, Korean Cell Line Bank, Korean Cell Line Research Foundation, Seoul, Republic of Korea.
authors.forename: J G
authors.initials: JG
authors.lastname: Park
authors.forename: H K
authors.initials: HK
authors.lastname: Yang
authors.forename: W H
authors.initials: WH
authors.lastname: Kim
authors.forename: J K
authors.initials: JK
authors.lastname: Chung
authors.forename: M S
authors.initials: MS
authors.lastname: Kang
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Oh
authors.forename: H S
authors.initials: HS
authors.lastname: Park
authors.forename: K S
authors.initials: KS
authors.lastname: Yeo
authors.forename: S H
authors.initials: SH
authors.lastname: Kang
authors.forename: S Y
authors.initials: SY
authors.lastname: Song
authors.forename: Y K
authors.initials: YK
authors.lastname: Kang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Bang
authors.forename: Y H
authors.initials: YH
authors.lastname: Kim
authors.forename: J P
authors.initials: JP
authors.lastname: Kim
mesh_terms: D000328:Adult; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology*