Levels and activity of brain protein kinase C alpha and zeta during the aging of the medfly.
Brain protein kinase C (PKC) activity, as well as PKC alpha and PKC zeta levels detected by immunoblotting, were monitored during the lifespan of the Mediterranean fruit-fly Ceratitis capitata. PKC activity increased in the particulate fraction during the last stages of the life of C. capitata. Immunoblotting studies with an anti-PKC alpha antibody also demonstrated increased enzyme levels in the particulate fraction. Cytosolic levels of PKC zeta decreased in the terminal phase of the lifespan of the fly, whereas levels of membrane-bound PKC zeta increased at that stage. Results thus indicate that during C. capitata final phase of life a translocation of PKC alpha and PKC zeta to the particulate fraction occurs, and therefore both kinases could be involved in the terminal process of this fruit-fly.
Galve-Roperh
I
I
Departamento de Bioquímica y Biologia Molecular I, Fac. Químicas, Universidad Complutense, Ciudad Universitaria, Madrid, Spain.
Malpartida
J M
JM
García-Barreno
P
P
Haro
A
A
Laviada
I D
ID
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D046508:Culture Techniques; D004175:Diptera; D007150:Immunohistochemistry; D011493:Protein Kinase C / Q000378:metabolism*
D011493:Protein Kinase C