title: High efficiency transformation of Bacillus brevis by electroporation.
abstract: Various conditions in the Bacillus brevis transformation by electroporation were investigated to increase the efficiency. Competent cell volume and presence of polyethylene glycol 6000 and single stranded DNA on pulsing were critical for the efficiency. As a result, we established the improved method by which 10(7)-10(8) transformants/microgram plasmid DNA could be obtained.
authors.affiliation: Department of Brewing and Fermentation, Tokyo University of Agriculture, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Okamoto
authors.forename: A
authors.initials: A
authors.lastname: Kosugi
authors.forename: Y
authors.initials: Y
authors.lastname: Koizumi
authors.forename: F
authors.initials: F
authors.lastname: Yanagida
authors.forename: S
authors.initials: S
authors.lastname: Udaka
mesh_terms: D001407:Bacillus / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D014169:Transformation, Bacterial
chemical_list: D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols