Amplification, cloning and sequencing of the sigmaC-encoded gene of avian reovirus.
The sigmaC-encoding cDNA of avian reovirus (ARV) 1733 strain was amplified, cloned and sequenced using double nested polymerase chain reaction (PCR). The ARV sigmaC protein is a minor component of the outer capsid that induces type-specific neutralization antibodies. Four overlapping sigmaC-encoding cDNA fragments were obtained. Together, the four fragments represented the whole coding sequence. The nucleotide and deduced amino acid sequences of sigmaC-encoded gene of U.S. (S1133 and 1733) and Australian isolates (RAM-1 and SOM-4) were compared. The U.S. isolates were closely related, but different from Australian isolates. The degree of differences between the U.S. and Australian isolates was over 44.89% at both the nucleotide and deduced amino acid levels and suggested that the virus is evolving separately in different continents. The deduced amino acid sequences of ARV sigmaC indicated a heptapeptide repeat in the N-terminal region of ARV sigmaC existed in all ARVs. The results suggested that ARV sigmaC is structurally related to mammalian reovirus (MRV) sigma1.
Liu
H J
HJ
Department of Poultry Science, Alabama Agriculture Experiment Station, Auburn University, 36849-5416, USA.
Giambrone
J J
JJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D008969:Molecular Sequence Data; D018165:Orthoreovirus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics*
D036022:Capsid Proteins; D004279:DNA, Viral; D016601:RNA-Binding Proteins; D014764:Viral Proteins; C053179:sigma protein 3, Reovirus