title: Characterization of post mortem arterial tissue using time-resolved photoacoustic spectroscopy at 436, 461 and 532 nm.
abstract: Time-resolved photoacoustic spectroscopy has been used to characterize post mortem arterial tissue for the purpose of discriminating between normal and atheromatous areas of tissue. Ultrasonic thermoelastic waves were generated in post mortem human aorta by the absorption of nanosecond laser pulses at 436, 461 and 532 nm produced by a frequency doubled Q-switched Nd:YAG laser in conjunction with a gas filled Raman cell. A PVDF membrane hydrophone was used to detect the thermoelastic waves. At 436 nm, differences in the photoacoustic signatures of normal tissue and atherorma were found to be highly variable. At 461 nm, there was a clear and reproducible difference between the photacoustic response of atheroma and normal tissue as a result of increased optical attenuation in atheroma. At 532 nm, the generation of subsurface thermoelastic waves provided a means of determining the structure and thickness of the tissue sample. It is suggested that pulsed photoacoustic spectroscopy at 461 and 532 nm may find application in characterizing arterial tissue in situ by providing information about both the composition and thickness of the vessel wall.
authors.affiliation: Department of Medical Physics and Bioengineering, University College London, UK.
authors.forename: P C
authors.initials: PC
authors.lastname: Beard
authors.forename: T N
authors.initials: TN
authors.lastname: Mills
mesh_terms: D001011:Aorta; D001158:Arteries / Q000473:pathology*; D001161:Arteriosclerosis / Q000473:pathology*; D002102:Cadaver; D004548:Elasticity; D006801:Humans; D007281:Ink; D007834:Lasers; D008962:Models, Theoretical; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods; D013997:Time Factors; D017539:Tunica Intima / Q000473:pathology; D017540:Tunica Media / Q000473:pathology