In vitro chemosensitivity of human pancreatic cancer cell lines.
CONCLUSIONS
These results show that eight pancreatic cancer cell lines are broadly sensitive to CDDP, and that chemotherapy for pancreatic cancer may improve the prognosis by more effective drug delivery to cancer cells.


BACKGROUND
Chemotherapy for pancreatic cancer does not satisfactorily improve prognosis. The efficacy of chemotherapy depends on choosing sensitive anticancer drugs.


METHODS
The in vitro chemosensitivity of eight human pancreatic cancer cell lines was investigated. Growth inhibition was measured by 3H-thymidine incorporation assays for doxorubicin hydrochloride (ADM), mitomycin C (MMC), cisplatin (CDDP), and etoposide (VP-16), and by Alamar Blue assay for (AB assay) 5-fluorouracil (5-FU). The cells were exposed to ADM, MMC, CDDP, and VP-16 for 2 h, and 5-FU for 72 h. From the dose-response curves, the 50% growth inhibition (IC50) level for each drug was estimated.


RESULTS
The IC50 after 2 h of exposure of each of the eight kinds of cell lines to each anticancer drug ranged from 0.12-8.2 micrograms/mL for ADM, 0.066-25 micrograms/mL for MMC, 0.57-7 micrograms/mL for CDDP, 0.68-300 micrograms/mL for VP-16. IC50 after 72 h of exposure to 5-FU ranged from 1.8-23 micrograms/mL.
Sawabe
Y
Y
Second Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Yamagishi
H
H
Yamaguchi
N
N
Yamamura
Y
Y
Oka
T
T
D000368:Aged; D000963:Antimetabolites / Q000633:toxicity; D000970:Antineoplastic Agents / Q000633:toxicity*; D018270:Carcinoma, Ductal, Breast / Q000188:drug therapy*; D002945:Cisplatin / Q000633:toxicity; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity; D005047:Etoposide / Q000633:toxicity; D005472:Fluorouracil / Q000633:toxicity; D006801:Humans; D008297:Male; D008875:Middle Aged; D016685:Mitomycin / Q000633:toxicity; D010190:Pancreatic Neoplasms / Q000188:drug therapy*; D013997:Time Factors; D018675:Toxicity Tests / Q000379:methods*; D014407:Tumor Cells, Cultured
D000963:Antimetabolites; D000970:Antineoplastic Agents; D016685:Mitomycin; D005047:Etoposide; D004317:Doxorubicin; D002945:Cisplatin; D005472:Fluorouracil