Early effects of basic fibroblast growth factor on foetal rat mesencephalic cell suspensions.
Mesencephalic cell suspensions are used, experimentally but also clinically, to compensate for neurological deficiencies, by implantation into the striatum. Here, we have studied the metabolism of mesencephalic cell suspensions obtained from rat embryos by measuring heat dissipation, oxygen consumption, ATP and lactate production. The effect of basic fibroblast growth factor (bFGF) at a 50 ng/ml concentration on these parameters was studied in order to assess the effect of in vitro exposure of cell suspensions to this trophic factor. Heat production and oxygen consumption were low, as could be expected from an immature nervous tissue, and they further decreased after addition of bFGF. This trophic factor decreased the total ATP concentration and increased the lactate production. The viability of the cell suspensions was reduced by nearly a half, 2 h after the addition of bFGF, and numerous fragmented nuclei were observed. It seems that, in contrast to the neuroprotective effect of bFGF on mesencephalic cultures and nigrostriatal neurons, this factor could have an initial sorting effect in the development of mesencephalic structures.
Espejo
M
M
Unitat de Bioquímica, Universitat de Barcelona, Spain.
Roig
T
T
Cutillas
B
B
Bermúdez
J
J
Bartrons
R
R
Ambrosio
S
S
D000165:Acridine Orange; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001562:Benzimidazoles; D002151:Calorimetry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D005333:Fetus / Q000166:cytology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005456:Fluorescent Dyes; D007773:Lactates / Q000378:metabolism; D008636:Mesencephalon / Q000166:cytology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats
D001562:Benzimidazoles; D005456:Fluorescent Dyes; D007773:Lactates; D016222:Fibroblast Growth Factor 2; D000255:Adenosine Triphosphate; D000165:Acridine Orange; C017807:bisbenzimide ethoxide trihydrochloride