title: Demonstration of the existence of mRNAs encoding N1/cif and N2/cit sodium/nucleoside cotransporters in rat brain.
abstract: Nucleoside transport may be involved in the regulation of extracellular levels of adenosine, an inhibitory neuromodulator in the central nervous system. Previous reports have provided functional evidence for Na+-dependent nucleoside transport in rat brain. We isolated total RNA from various regions of rat brain and tested for the presence of mRNA for two recently cloned Na+/nucleoside cotransporters using reverse transcriptase PCR (RT-PCR). Messenger RNA for a pyrimidine-selective Na+/nucleoside cotransporter mRNA (rCNT1) was detected in samples from each brain region tested by RT-PCR amplification of a 309-bp DNA product. Southern blot and sequence analysis confirmed that this product was derived from rCNT1 mRNA. A purine-selective Na+/nucleoside cotransporter mRNA (rCNT2, also termed SPNT) was detected throughout brain by amplifying a 235-bp DNA product, the sequence of which was identical to that published. These experiments demonstrate the presence of both rCNT1 and rCNT2 mRNA in rat brain.
authors.affiliation: Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
authors.forename: C M
authors.initials: CM
authors.lastname: Anderson
authors.forename: W
authors.initials: W
authors.lastname: Xiong
authors.forename: J D
authors.initials: JD
authors.lastname: Young
authors.forename: C E
authors.initials: CE
authors.lastname: Cass
authors.forename: F E
authors.initials: FE
authors.lastname: Parkinson
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics*; D008297:Male; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D011684:Purine Nucleosides / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D011684:Purine Nucleosides; D012333:RNA, Messenger; C065171:cif nucleoside transporter; D012964:Sodium