title: Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons.
abstract: Membrane excitability in different tissues is due, in large part, to the selective expression of distinct genes encoding the voltage-dependent sodium channel. Although the predominant sodium channels in brain, skeletal muscle, and cardiac muscle have been identified, the major sodium channel types responsible for excitability within the peripheral nervous system have remained elusive. We now describe the deduced primary structure of a sodium channel, peripheral nerve type 1 (PN1), which is expressed at high levels throughout the peripheral nervous system and is targeted to nerve terminals of cultured dorsal root ganglion neurons. Studies using cultured PC12 cells indicate that both expression and targeting of PN1 is induced by treatment of the cells with nerve growth factor. The preferential localization suggests that the PN1 sodium channel plays a specific role in nerve excitability.
authors.affiliation: Department of Neurobiology and Behavior, State University of New York at Stony Brook, 11794-5230, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Toledo-Aral
authors.forename: B L
authors.initials: BL
authors.lastname: Moss
authors.forename: Z J
authors.initials: ZJ
authors.lastname: He
authors.forename: A G
authors.initials: AG
authors.lastname: Koszowski
authors.forename: T
authors.initials: T
authors.lastname: Whisenand
authors.forename: S R
authors.initials: SR
authors.lastname: Levinson
authors.forename: J J
authors.initials: JJ
authors.lastname: Wolf
authors.forename: I
authors.initials: I
authors.lastname: Silos-Santiago
authors.forename: S
authors.initials: S
authors.lastname: Halegoua
authors.forename: G
authors.initials: G
authors.lastname: Mandel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002451:Cell Compartmentation; D018076:DNA, Complementary / Q000235:genetics; D005727:Ganglia, Spinal / Q000737:chemistry* / Q000166:cytology; D015870:Gene Expression; D015723:Gene Library; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D062556:NAV1.7 Voltage-Gated Sodium Channel; D016501:Neurites / Q000737:chemistry; D009474:Neurons / Q000737:chemistry*; D009479:Neuropeptides / Q000145:classification / Q000235:genetics*; D016716:PC12 Cells; D017933:Peripheral Nervous System / Q000737:chemistry* / Q000166:cytology; D016133:Polymerase Chain Reaction; D051381:Rats; D015222:Sodium Channels / Q000145:classification / Q000235:genetics*; D013569:Synapses / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D062556:NAV1.7 Voltage-Gated Sodium Channel; D009479:Neuropeptides; C503480:Scn9a protein, rat; D015222:Sodium Channels