Is wheat germ agglutinin (WGA) a glycosylated protein?
Lectin isolated from wheat germ was purified by affinity chromatography on ovogel column, recrystallization and gel filtration on Ultrogel AcA 44 column, eluted with borate buffer to eliminate accidental lectin-ligand interaction products. In 87-times purified WGA preparation fucose, xylose, mannose, galactose and glucose were found, amounting 1.5% of lectin mass.
Kisiel
D G
DG
Department of General and Organic Chemistry, Medical Academy of Białystok.
Gindzieński
A
A
Zwierz
K
K
D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D014909:Wheat Germ Agglutinins / Q000032:analysis*
D014909:Wheat Germ Agglutinins