Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis.
A genetic typing method for the mouse obese (ob) mutation by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Three genotypes (ob/ob, ob/+ and +/+) of the ob mouse are rapidly differentiated with this assay. Since only a small biopsy specimen (tail end) is necessary for the genotyping, the ob mouse at any age is typable and can be used in subsequent breeding or research. Obese homozygotes (ob/ob) were efficiently produced by mating heterozygotes (ob/+) only, which were selected by using the PCR-RFLP assay.
Hirasawa
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T
Center for Experimental Animals Development, Shionogi & Co., Ltd., Shiga, Japan.
Ohara
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T
Makino
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S
D000818:Animals; D004262:DNA Restriction Enzymes; D005838:Genotype; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008820:Mice, Obese / Q000235:genetics*; D009154:Mutation; D009765:Obesity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004262:DNA Restriction Enzymes