title: Morphometry and autoradiography of altered rat enamel protein processing due to chronic exposure to fluoride.
abstract: Female Sprague-Dawley rats had 6 weeks of 0 (control), 75 or 100 parts/10(6) sodium fluoride in their drinking water. Whole mandibular incisors were removed, fixed, demineralized and sections prepared for light-microscopic morphometric analysis of dose-related alterations in enamel protein retention. Other rats given 0 and 75 parts/10(6) only (control and experimental groups) were used for autoradiographic evaluation of alterations in enamel protein removal 35S-methionine was applied directly over secretory ameloblasts at the end of the fifth week of fluoride exposure. Incisors were removed either 5 or 7 days later and processed for autoradiographic analysis. The results indicated: (1) extended retention of enamel proteins in fluoride-exposed maturation enamel as well as reduced enamel protein synthesis and/or secretion in the secretory stage; (2) negative linear correlation between extended enamel protein retention and reduced enamel protein secretion among groups; and (3) repression of enamel protein removal. The data are also consistent with the concept that the fluoride effect is multifactorial.
authors.affiliation: Department of Oral Biology, University of Illinois at Chicago 60612, USA.
authors.forename: R
authors.initials: R
authors.lastname: Zhou
authors.forename: A E
authors.initials: AE
authors.lastname: Zaki
authors.forename: D R
authors.initials: DR
authors.lastname: Eisenmann
mesh_terms: D000565:Ameloblasts / Q000187:drug effects* / Q000378:metabolism; D000566:Amelogenesis / Q000187:drug effects; D000704:Analysis of Variance; D000818:Animals; D001345:Autoradiography; D003743:Dental Enamel / Q000187:drug effects*; D003746:Dental Enamel Proteins / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005459:Fluorides / Q000494:pharmacology*; D009050:Fluorosis, Dental / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003746:Dental Enamel Proteins; D005459:Fluorides