title: Mutational analysis of the feedback sites of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli.
abstract: In Escherichia coli, aroF, aroG, and aroH encode 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase isozymes that are feedback inhibited by tyrosine, phenylalanine, and tryptophan, respectively. In vitro chemical mutagenesis of the cloned aroG gene was used to identify residues and regions of the polypeptide essential for phenylalanine feedback inhibition.
authors.affiliation: Central Research Laboratory, Ajinomoto Co., Inc., Kawasaki, Japan. lt_kikuchi@te10.ajinomoto.co.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: K
authors.initials: K
authors.lastname: Tsujimoto
authors.forename: O
authors.initials: O
authors.lastname: Kurahashi
mesh_terms: D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000187:drug effects / Q000235:genetics*; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005246:Feedback; D007527:Isoenzymes / Q000187:drug effects / Q000235:genetics*; D010649:Phenylalanine / Q000096:biosynthesis / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D014364:Tryptophan / Q000096:biosynthesis; D014443:Tyrosine / Q000096:biosynthesis
chemical_list: D007527:Isoenzymes; D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan; D010723:3-Deoxy-7-Phosphoheptulonate Synthase