Characterization of the 5' flanking region of the human complement factor H gene.
The promoter region of the human factor H gene was cloned and a 3 kb Eco RI fragment was sequenced. Primer extension and S1 nuclease analysis were used to determine the transcription start site, which was found to be 10-11 nucleotides upstream of the published cDNA sequence. No canonical TATA or CCAAT boxes were found in conjunction with this site. The sequence from the human H promoter region was compared to that from the mouse gene. There was a region of 800 bp that was 62.5% identical between the two sequences. The sequences of the two promoter regions were compared to a database of transcription factor binding sites. Five elements were identified that matched the consensus sequence 100% and were identical in the two promoter sequences. Promoter assays using the luciferase reporter gene demonstrated that this region contained a functional transcription start site and putative enhancer elements. U118-MG astroglioma cells and Hep3b hepatoma cells were incubated with various cytokines to measure effects on their factor H mRNA levels. Interferon-gamma (IFN-gamma), but not interleukin-1 (IL-1), tumour necrosis factor alpha (TNF-alpha) or IL-6, was able to increase the level of H mRNA in both cell lines.
Williams
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Department of Microbiology and Immunology, University of New Mexico School of Medicine, Albuquerque 87131-5276, USA.
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D000818:Animals; D001254:Astrocytoma; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D003001:Cloning, Molecular; D017242:Complement Factor H / Q000235:genetics*; D016207:Cytokines / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
C493495:CFH protein, human; D016207:Cytokines; D017242:Complement Factor H