Cloricromene reduces myocardial infarct size in rabbits when administered during the early reperfusion period.
Cloricromene is a coumarin derivative without anticoagulant activities that has recently been found to decrease myocardial infarct size after an ischemic-reperfusion injury. This study seeks to determine when the cardioprotective action of cloricromene is exerted in an in vivo rabbit model of ischemic-reperfusion injury. Forty-nine rabbits subjected to 30 min of coronary occlusion and 120 min of reperfusion were randomized into five groups: VEH (n = 11) received saline vehicle; IR (n = 9) received an infusion of cloricromene starting at the onset of ischemia at 8 micrograms.kg-1.min-1; R(-5)(n = 9) and R(+30)(n = 9) received an infusion of cloricromene at 8 micrograms.kg-1.min-1 starting 5 min before reperfusion and 30 min after reperfusion, respectively; and RB(-5)(n = 11) received 300 micrograms/kg bolus of cloricromene 5 min before reperfusion followed by an infusion of 8 micrograms.kg-1.min-1. All infusions were continued until the end of the reperfusion period. Myocardial infarct size was significantly reduced in groups IR, R(-5), and RB(-5). We conclude that cloricromene's effective time of action occurs prior to the first 30 min of the reperfusion period.
Zvara
D A
DA
Department of Anesthesia, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27157-1009, USA.
Galaska
H J
HJ
Castellano
V P
VP
Vinten-Johansen
J
J
Royster
R L
RL
Williams
M W
MW
Murphy
B A
BA
Kim
E J
EJ
D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage*; D001794:Blood Pressure; D002866:Chromonar / Q000008:administration & dosage / Q000031:analogs & derivatives*; D003374:Coumarins / Q000008:administration & dosage*; D006339:Heart Rate; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology; D011817:Rabbits; D013997:Time Factors
D000925:Anticoagulants; D003374:Coumarins; C025945:cloricromen; D002866:Chromonar