The anti-tumoral activity of human glycoprotein HGP.92: a study with the mouse Lewis-lung-tumor cell.
The ability of a purified human glycoprotein (HGP.92) to exert anti-tumor activity was investigated in a mouse model using long-term readout assays. In vitro, in the presence of inflammatory mouse macrophages incubated with HGP.92, the growth of the mouse Lewis-lung-tumor cells (3LL) was decreased. This effect was concentration-dependent and required direct contact between tumor targets and HGP.92-treated macrophages. In addition, if the macrophage monolayer was depleted of HGP.92 before addition of the target cells, no more cytostatic effect was observed. This anti-tumor activity of HGP.92-treated mouse macrophage was partially abrogated by addition of catalase in the culture medium, but not by superoxide dismutase or scavengers of the hydroxyl radical and singlet oxygen. Moreover, this tumor-cell growth reduction was not dependent on nitric oxide. In vivo, multiple i.v. injections of HGP.92 (5 times, 3 days apart) during the first week and a half exerted significant anti-tumor activity, as assessed by the reduction of both the number and the size of the lung nodules 3 weeks after i.v. inoculation of 3LL cells.
Fontan
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Unité d'Immunophysiologie Cellulaire, Institut Pasteur, Paris, France. efontan@pasteur.fr
Briend
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Saklani-Jusforgues
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H
Fauve
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RM
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use*; D018827:Carcinoma, Lewis Lung / Q000628:therapy*; D002450:Cell Communication; D004340:Drug Contamination; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009088:Mucoproteins / Q000737:chemistry / Q000627:therapeutic use*; D009569:Nitric Oxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors; D058949:Uromodulin
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D008070:Lipopolysaccharides; D009088:Mucoproteins; D017382:Reactive Oxygen Species; C548612:UMOD protein, human; C548613:Umod protein, mouse; D058949:Uromodulin; D009569:Nitric Oxide