Developmental regulation of alpha-tubulin mRNAs during the differentiation of cultured cerebellar granule cells.
T alpha 1 and T26 alpha-tubulin mRNA expression was examined during the differentiation of rat cerebellar granule cells in vitro and in situ. High levels of T alpha 1 transcript correlated with neurons extending processes and hence may implicate T alpha 1 with neuritogenesis. In comparison, T26 labeling was much less prominent, appeared more constitutive and was possibly associated with cell proliferation. Such profiles indicate that the different isotypes play different roles in the assembly and function of microtubules during neuronal differentiation.
Przyborski
S A
SA
Department of Biomedical Science, University of Sheffield, Western Bank, UK.
Cambray-Deakin
M A
MA
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D014404:Tubulin / Q000235:genetics*
D012333:RNA, Messenger; D014404:Tubulin