title: Temporins, antimicrobial peptides from the European red frog Rana temporaria.
abstract: A cDNA library from the skin of Rana temporaria has been screened using a cDNA fragment probe that encodes the signal peptide of the precursor of esculentin from the skin secretion of Rana esculenta. With this approach, the cDNAs encoding the precursors of three peptides were isolated. Subsequently, the peptides predicted from the sequence of the cloned cDNAs as well as several structurally related peptides could be isolated from the skin secretion of R. temporaria. These peptides, which were named temporins, have a length of 10-13 residues and show some sequence similarity to hemolytic peptides isolated from Vespa venom [Argiolas, A. & Pisano, J. J. (1984) J. Biol. Chem. 259, 10106-10111]. Natural and synthetic temporins have antibacterial activity against gram-positive bacteria, but they are not hemolytic. Temporins are the smallest antibacterial peptides hitherto found in nature.
authors.affiliation: Istituto Pasteur-Fondazione Cenci Bolognetti, Università La Sapienza, Roma, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Simmaco
authors.forename: G
authors.initials: G
authors.lastname: Mignogna
authors.forename: S
authors.initials: S
authors.lastname: Canofeni
authors.forename: R
authors.initials: R
authors.lastname: Miele
authors.forename: M L
authors.initials: ML
authors.lastname: Mangoni
authors.forename: D
authors.initials: D
authors.lastname: Barra
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D011896:Rana temporaria; D012867:Skin / Q000737:chemistry
chemical_list: D029845:Amphibian Proteins; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D010455:Peptides; D011498:Protein Precursors; D011506:Proteins; C092874:esculentin protein, Rana esculenta; C104501:temporin