title: Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome.
abstract: The tylLM region of the tylosin biosynthetic gene cluster of Streptomyces fradiae contains four open reading frames (orfs1*-4*). The function of the orf1* product is not known. The product of orf2* (tylM2) is the glycosyltransferase that adds mycaminose to the 5-hydroxyl group of tylactone, the polyketide aglycone of tylosin (Ty). A methyltransferase, responsible for 3-N-methylation during mycaminose production, is encoded by orf3* (tylM1). The product of orf4* (cer) is crotonyl-CoA reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender unit during tylactone production.
authors.affiliation: Department of Biochemistry, University of Leicester, UK.
authors.forename: A R
authors.initials: AR
authors.lastname: Gandecha
authors.forename: S L
authors.initials: SL
authors.lastname: Large
authors.forename: E
authors.initials: E
authors.lastname: Cundliffe
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005944:Glucosamine / Q000031:analogs & derivatives / Q000096:biosynthesis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D005810:Multigene Family; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D015645:Tylosin / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C013610:mycaminose; D005944:Glucosamine; D015645:Tylosin