Characterization of oligodeoxyribonucleotide-polyethylene glycol conjugates by electrospray mass spectrometry.
Electrospray mass spectrometry (ESMS) was used to characterize a number of differently functionalized polyethylene glycol-oligodeoxyribonucleotide conjugates. Sample preparation was found to be crucial to obtaining quality data. The resolution and precision of ESMS allowed for the identification of individual conjugates differing in MW by a single ethylene glycol unit with an accuracy of < or = 1 (0.02% of the molecular weight). In addition, ESMS was shown to be valuable in identifying chromatographically unresolvable components of a derivatized polyethylene glycol-oligonucleotide conjugate mixture.
Tarasow
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TM
NeXstar Pharmaceuticals Inc., Boulder, Colorado 80301, USA.
Tinnermeier
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D
Zyzniewski
C
C
D000577:Amides / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D009838:Oligodeoxyribonucleotides; D063088:Phosphoramides; D010756:Phosphoric Acids / Q000378:metabolism; D011092:Polyethylene Glycols
D000577:Amides; D009838:Oligodeoxyribonucleotides; D063088:Phosphoramides; D010756:Phosphoric Acids; C005438:phosphoramide; D011092:Polyethylene Glycols