Positional cloning of a gene for nematode resistance in sugar beet.
The Hs1(pro-1) locus confers resistance to the beet cyst nematode (Heterodera schachtii Schmidt), a major pest in the cultivation of sugar beet (Beta vulgaris L.). The Hs1(pro-1) gene was cloned with the use of genome-specific satellite markers and chromosomal break-point analysis. Expression of the corresponding complementary DNA in a susceptible sugar beet conferred resistance to infection with the beet cyst nematode. The native Hs1(pro-1) gene, expressed in roots, encodes a 282-amino acid protein with imperfect leucine-rich repeats and a putative membrane-spanning segment, features similar to those of disease resistance genes previously cloned from higher plants.
Cai
D
D
Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany.
Kleine
M
M
Kifle
S
S
Harloff
H J
HJ
Sandal
N N
NN
Marcker
K A
KA
Klein-Lankhorst
R M
RM
Salentijn
E M
EM
Lange
W
W
Stiekema
W J
WJ
Wyss
U
U
Grundler
F M
FM
Jung
C
C
D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D005816:Genetic Complementation Test; D007930:Leucine / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009348:Nematoda / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology; D010940:Plant Proteins; D018517:Plant Roots / Q000235:genetics / Q000469:parasitology; D014170:Transformation, Genetic; D014675:Vegetables / Q000235:genetics* / Q000469:parasitology*
D018076:DNA, Complementary; C103918:Hs1 pro-1 protein, Beta procumbens; D008565:Membrane Proteins; D010940:Plant Proteins; D007930:Leucine