Neutralizing activity and antibody reactivity toward immunogenic regions of the human T cell leukemia virus type I surface glycoprotein in sera of infected patients with different clinical states.
The induction of specific neutralizing antibodies is an important part of vaccine strategy against human T cell leukemia virus type I (HTLV-I). A recently developed reporter gene induction assay was used to detect and quantify neutralizing antibodies in sera of HTLV-I-infected patients with different clinical states: Most sera (73/89) displayed an inhibitory activity. Neutralizing antibodies were more frequently detected in sera of patients with tropical spastic paraparesis/HTLV-associated myelopathy (TSP/HAM) or sicca syndrome (SS) (100%) than in sera of patients with adult T cell leukemia (ATL; 50%) or of asymptomatic carriers (AS; 83%). The mean titers in the different groups were significantly different (ATL < AS < TSP/HAM and SS). The antibody reactivity detected by the reporter gene inhibition assay was significantly related to the recognition of the neutralizable immunodominant domain (aa 175-199) of the surface envelope glycoprotein, indicating the importance of this region for potential vaccines.
Astier-Gin
T
T
INSERM, Université de Bordeaux II, France.
Portail
J P
JP
Londos-Gagliardi
D
D
Moynet
D
D
Blanchard
S
S
Dalibart
R
R
Pouliquen
J F
JF
Georges-Courbot
M C
MC
Hajjar
C
C
Sainte-Foie
S
S
Guillemain
B
B
D018604:Epitope Mapping; D015686:Gene Products, env / Q000276:immunology*; D015481:HTLV-I Antibodies / Q000276:immunology*; D015486:HTLV-I Antigens / Q000276:immunology*; D015490:HTLV-I Infections / Q000276:immunology*; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D015458:Leukemia, T-Cell / Q000276:immunology; D009500:Neutralization Tests; D015493:Paraparesis, Tropical Spastic / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology*; D012859:Sjogren's Syndrome / Q000276:immunology
D015686:Gene Products, env; D015481:HTLV-I Antibodies; D015486:HTLV-I Antigens; D015682:Retroviridae Proteins, Oncogenic; C055266:gp46 protein, Human T-cell leukemia virus type I