Influence of blood sample oxygen tension on blood glucose concentration measured using an enzyme-electrode method.
OBJECTIVE
To determine the accuracy of a bedside glucometer with an enzyme-electrode sensor based on enzyme oxidation by glucose oxidase.


METHODS
Prospective, cross-sectional clinical study.


METHODS
Operating room in a public hospital.


METHODS
Fifty-four patients undergoing surgical procedures for a derivation (n = 17) and a validation (n= 37) study.


METHODS
Arterial blood samples were obtained via a 20-gauge cannula inserted into each patient's radial artery.


RESULTS
Glucose measurements and arterial blood gas analyses were concurrently performed, using 48 blood samples for the derivation study and 45 blood samples for the validation study of this technique. Blood glucose concentrations were measured with both a bedside glucometer using an enzyme-electrode method and a laboratory glucometer based on the colorimetric method. The bedside glucometer consistently underestimated the glucose concentrations and the underestimation was related to the sample oxygen tension but not to hematocrit, plasma protein, creatinine, uric acid, or bilirubin. The present investigation used the following correction formula: (corrected glucose value) = (glucose concentration obtained by a bedside glucometer) + 0.1 x (sample oxygen tension) + 16. The corrected data were in agreement with the laboratory-determined glucose values (i.e., the mean difference and precision were 0.4 and 7.1 mg/dL, respectively). A validation study confirmed the generalization of the present correction formula which facilitates a more accurate estimation of blood glucose concentrations.


CONCLUSIONS
Blood glucose values measured using a bedside glucometer in this study were influenced by the sample oxygen tension. We used a corrective equation which improved the accuracy of estimating blood glucose values to a clinically acceptable range.
Kurahashi
K
K
Department of Anesthesia, Odawara Municipal Hospital, Japan.
Maruta
H
H
Usuda
Y
Y
Ohtsuka
M
M
D000328:Adult; D001784:Blood Gas Analysis; D001786:Blood Glucose / Q000187:drug effects*; D004566:Electrodes; D005260:Female; D005949:Glucose Oxidase / Q000494:pharmacology; D006801:Humans; D007362:Intensive Care Units; D008297:Male; D019095:Point-of-Care Systems; D015203:Reproducibility of Results
D001786:Blood Glucose; D005949:Glucose Oxidase