Reversible inhibition of lambda exonuclease with high pressure.
The hydrolytic activity of lambda exonuclease, a highly processive enzyme, is inhibited by high pressure. When assayed at 67 MPa, the activity is 87% of the atmospheric rate; increasing the pressure to 336 MPa causes a greater than 99% inhibition of the enzyme activity. Decreasing the pressure from 336 MPa to 67 MPa at 20 degrees C reverses the inhibition. The use of hydrostatic pressure to control the activity of lambda exonuclease has potential practical application in DNA sample preparation, analysis, and sequencing.
Rudd
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EA
BioSeq, Inc., Woburn, Massachusetts 01801-6307, USA.
D001274:Atmospheric Pressure; D004247:DNA / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D007700:Kinetics; D011312:Pressure; D013379:Substrate Specificity; D014764:Viral Proteins
D014764:Viral Proteins; D004247:DNA; D005090:Exodeoxyribonucleases; C043586:exo protein, Bacteriophage lambda