title: The distal limb environment regulates MyoD accumulation and muscle differentiation in mouse-chick chimaeric limbs.
abstract: Differentiation of muscle and cartilage within developing vertebrate limbs occurs in a proximodistal progression. To investigate the cues responsible for regulating muscle pattern, mouse myoblasts were implanted into early chick wings prior to endogenous chick muscle differentiation. Fetal myogenic cells originating from transgenic mice carrying a lacZ reporter were readily detected in vivo after implantation and their state of differentiation determined with species-specific antibodies to MyoD and myosin heavy chain. When mouse myogenic cells are implanted at the growing tip of early stage 21 limbs MyoD expression is suppressed and little differentiation of the mouse cells is detected initially. At later stages ectopically implanted mouse cells come to lie within muscle masses, re-express MyoD and differentiate in parallel with differentiating chick myoblasts. However, if mouse cells are implanted either proximally at stage 21 or into the limb tip at stage 24, situations in which mouse cells encounter endogenous differentiating chick myoblasts earlier, MyoD suppression is not detected and a higher proportion of mouse cells differentiate. Mouse cells that remain distal to endogenous differentiating myogenic cells are more likely to remain undifferentiated than myoblasts that lie within differentiated chick muscle. Undifferentiated distal mouse cells are still capable of differentiating if explanted in vitro, suggesting that myoblast differentiation is inhibited in vivo. In vitro, MyoD is suppressed in primary mouse myoblasts by the addition of FGF2 and FGF4 to the culture media. Taken together, our data suggest that the inhibition of myogenic differentiation in the distal limb involves MyoD suppression in myoblasts, possibly through an FGF-like activity.
authors.affiliation: MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, The Randall Institute, King's College London, UK.
authors.forename: L G
authors.initials: LG
authors.lastname: Robson
authors.forename: S M
authors.initials: SM
authors.lastname: Hughes
mesh_terms: D000818:Animals; D019521:Body Patterning; D002450:Cell Communication; D002454:Cell Differentiation; D002642:Chick Embryo; D002999:Clone Cells; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008808:Mice, Inbred CBA; D009132:Muscles / Q000166:cytology / Q000196:embryology* / Q000637:transplantation; D017570:MyoD Protein / Q000096:biosynthesis*; D013234:Stem Cells; D013997:Time Factors; D018183:Transplantation Chimera; D014921:Wings, Animal / Q000196:embryology
chemical_list: D017570:MyoD Protein