title: Identification of a T lineage antigen in the catfish.
abstract: A murine monoclonal antibody produced against catfish thymocytes and immunoglobulin-negative lymphocytes in the blood identified a catfish T cell antigen designated CfT1. The CfT1 antigen was found to be expressed on thymocytes, a subpopulation of the lymphoid cells in blood and other lympho-hemopoietic tissues, and a T cell line, but was not expressed by erythrocytes, thrombocytes, myeloid cells, B cells or macrophage cell lines. Stimulation of blood mononuclear cells with the T cell mitogen, concanavalin A, resulted in an increased frequency of CfT1+ cells. Conversely, lipopolysaccharide stimulation increased the number of IgM+ B cells and decreased the frequency of CfT1+ cells. The CfT1 antigen was defined as a single chain protein of M(r) 35,000 lacking N- and O-linked sugars. The CfT1 molecule thus provides a T lineage-specific marker in this bony fish representative.
authors.affiliation: Department of Microbiology, University of Alabama at Birmingham 35294-3300, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Passer
authors.forename: C H
authors.initials: CH
authors.lastname: Chen
authors.forename: N W
authors.initials: NW
authors.lastname: Miller
authors.forename: M D
authors.initials: MD
authors.lastname: Cooper
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D002460:Cell Line; D007059:Ictaluridae; D007120:Immunochemistry; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D009928:Organ Specificity / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D008934:Mitogens