title: P53 gene mutations in osteosarcomas in the dog.
abstract: Osteosarcomas in 18 dogs were examined for the presence of p53 mutations in exons 4-8 by single strand conformation polymorphism (SSCP) analysis, followed by sequence analysis in tumors demonstrating abnormal bands in the SSCP analysis. P53 mutations were found in four of the primary tumors in 17 dogs. Metastases studied in two of these dogs in which the primary tumor contained only wild type p53 did not contain mutations, nor those of one dog in which the primary tumor was not studied. The alterations that were found included three missense mutations and one 3 bp insertion.
authors.affiliation: Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
authors.forename: I S
authors.initials: IS
authors.lastname: van Leeuwen
authors.forename: C J
authors.initials: CJ
authors.lastname: Cornelisse
authors.forename: W
authors.initials: W
authors.lastname: Misdorp
authors.forename: S A
authors.initials: SA
authors.lastname: Goedegebuure
authors.forename: J
authors.initials: J
authors.lastname: Kirpensteijn
authors.forename: G R
authors.initials: GR
authors.lastname: Rutteman
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000662:veterinary*; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D016158:Genes, p53 / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics / Q000662:veterinary*; D018807:Polymorphism, Single-Stranded Conformational