title: Interleukin-2 (IL-2) and IL-6 regulate c-fos protooncogene expression in human pituitary adenoma explants.
abstract: We have previously shown that interleukin-2 (IL-2) and IL-6, which are expressed in the anterior pituitary, affect anterior pituitary cell proliferation in normal rats and cell lines. Here we examined their effects on the c-fos expression by human anterior pituitary adenomas. Adenoma cells in culture do not express c-fos mRNA. In adenoma explants, however, c-fos expression was detected and was regulated by IL-2 or IL-6. In different tumors (ACTH-, PRL-, GH-secreting and non functioning adenomas), these interleukins had inhibitory or stimulatory effects but the kind of response does not seem to be associated to tumor type or size. Using blocking antibodies, we observed that intrinsic IL-2 and IL-6 regulate c-fos expression in the same way. Our data suggest that IL-2 and IL-6 are not only involved in the regulation of pituitary adenoma function but may also, given the role of c-fos in cell proliferation, be implicated in the development of human pituitary adenomas.
authors.affiliation: Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors.forename: M P
authors.initials: MP
authors.lastname: Pereda
authors.forename: V
authors.initials: V
authors.lastname: Goldberg
authors.forename: A
authors.initials: A
authors.lastname: Chervín
authors.forename: G
authors.initials: G
authors.lastname: Carrizo
authors.forename: A
authors.initials: A
authors.lastname: Molina
authors.forename: J
authors.initials: J
authors.lastname: Andrada
authors.forename: J
authors.initials: J
authors.lastname: Sauer
authors.forename: U
authors.initials: U
authors.lastname: Renner
authors.forename: G K
authors.initials: GK
authors.lastname: Stalla
authors.forename: E
authors.initials: E
authors.lastname: Arzt
mesh_terms: D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D016762:Genes, fos / Q000235:genetics*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D010903:Pituitary Gland, Anterior; D010911:Pituitary Neoplasms / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D007376:Interleukin-2; D015850:Interleukin-6; D012333:RNA, Messenger; D012334:RNA, Neoplasm