Effects of adjuvant on neuropeptide-like immunoreactivity in experimentally induced temporomandibular arthritis in rats.
Substance P (SP)-, neurokinin A (NKA)-, calcitonin gene-related peptide (CGRP)- and neuropeptide Y (NPY)-like immunoreactivities (-LI) were examined in cerebrospinal fluid (CSF), plasma and temporomandibular joint (TMJ) perfusates in rats 1 and 12 h after inoculation at the base of the tail (0.05 ml) or injection into the right TMJ (0.01 ml) of heat-killed Mycobacterium butyricum in paraffin oil. In the rats inoculated at the base of the tail (polyarthritic rats), there was a significant increase of CGRP-LI and NKA-LI. The changes in neuropeptide-LI were not as marked in the CSF of rats injected with adjuvant in one TMJ (monoarthritic rats) as in the polyarthritic group. Instead, the most significant changes in the monoarthritic rats were seen in the perfusates of both TMJs. The increases in SP-, NKA-, CGRP- and NPY-LI were significant for both TMJs and more pronounced than in the polyarthritic rats. The results show that inoculation of adjuvant at the base of the tail induces significant changes of neuropeptide-LI predominantly in CSF, whilst an intra-articular injection induces bilateral changes in neuropeptide-LI in joint perfusate. Therefore, two different neural mechanisms may be involved early in adjuvant-induced poly- and monoarthritis.
Carleson
J
J
Department of Physiology and Pharmacology, Division of Physiology II, Stockholm, Sweden.
Alstergren
P
P
Appelgren
A
A
Appelgren
B
B
Kopp
S
S
Srinivasan
G R
GR
Theodorsson
E
E
Lundeberg
T
T
D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000276:immunology; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D009161:Mycobacterium; D015213:Neuroimmunomodulation / Q000502:physiology*; D015288:Neurokinin A / Q000032:analysis; D009478:Neuropeptide Y / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D013373:Substance P / Q000032:analysis; D013582:Synovial Fluid / Q000737:chemistry; D013705:Temporomandibular Joint Disorders / Q000276:immunology* / Q000378:metabolism
D001426:Bacterial Proteins; D009478:Neuropeptide Y; D009479:Neuropeptides; D013373:Substance P; D015288:Neurokinin A; D015740:Calcitonin Gene-Related Peptide