Molecular cloning and immunological characterisation of Cyn d 7, a novel calcium-binding allergen from Bermuda grass pollen.
A cDNA coding for a newly identified Bermuda grass pollen allergen, Cyn d 7, with significant sequence similarity to Ca2+-binding proteins, was isolated from a cDNA expression library using serum IgE from an allergic individual. The deduced amino acid sequence of Cyn d 7 contained two typical Ca2+-binding sites (EF hand domains). Depletion of Ca2+ with EGTA led to a loss of IgE-binding capacity of rCyn d 7. A synthetic peptide based on domain II showed high IgE reactivity. Cyn d 7 therefore represents a grass pollen allergen that belongs to a novel class of Ca2+-binding proteins.
Suphioglu
C
C
School of Botany, University of Melbourne, Parkville, Victoria, Australia. C.Suphioglu@botany.unimelb.edu.au
Ferreira
F
F
Knox
R B
RB
D000485:Allergens; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D003001:Cloning, Molecular; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D010940:Plant Proteins; D006109:Poaceae / Q000276:immunology* / Q000378:metabolism; D011058:Pollen; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D052179:Antigens, Plant; D002135:Calcium-Binding Proteins; C104593:Cyn d 7 protein, Cupressus arizonica; D010455:Peptides; D010940:Plant Proteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D002118:Calcium