Regulated system for heterologous gene expression in Penicillium chrysogenum.
A system for regulated heterologous gene expression in the filamentous fungus Penicillium chrysogenum was established. This is the first heterologous expression system to be developed for this organism. Expression of a recombinant fungal xylanase gene (xylp) and the cDNA for the human tear lipocalin (LCNI) was achieved by placing the encoding sequences under the control of the repressible acid phosphatase gene (phoA) promoter of P. chrysogenum. Secreted recombinant proteins were detected in the growth media of transformed P. chrysogenum cells by means of bioassays, zymogramography, and Western blotting. Levels of transcription and amounts of recombinant proteins secreted varied among transformants, mainly due to the copy number and the integration site of the expression vector on the fungal chromosome.
Graessle
S
S
Institut für Mikrobiologie (Medizinische Fakultät), Universität Innsbruck, Austria.
Haas
H
H
Friedlin
E
E
Kürnsteiner
H
H
Stöffler
G
G
Redl
B
B
D002352:Carrier Proteins / Q000096:biosynthesis / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D005656:Fungal Proteins / Q000096:biosynthesis; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D006801:Humans; D054835:Lipocalin 1; D010408:Penicillium chrysogenum / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism; D011995:Recombination, Genetic; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis / Q000378:metabolism; D014170:Transformation, Genetic; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000096:biosynthesis / Q000378:metabolism
D002352:Carrier Proteins; D005656:Fungal Proteins; C518443:LCN1 protein, human; D054835:Lipocalin 1; D011994:Recombinant Proteins; D012471:Salivary Proteins and Peptides; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase