title: P-element inserts in transgenic flies: a cautionary tale.
abstract: The fruitfly, Drosophila melanogaster, can be transfected with P-elements and induced to overexpress a transfected gene whose impact on lifespan can be measured. Here, it is reported that in previous experiments a transfected gene was not expressed. This suggested a new statistical analysis indicating that (1) the size of the insert, the position and the interactions of the insert with the genetic backgrounds into which the P-element are inserted have effects on lifespan similar to those attributed to overexpression; (2) these effects occur without expression of the transfected gene; and (3) effects of interactions with backgrounds and effects of positions are as large as responses to six generations of strong directional artificial selection. Reports of effects of overexpression of transfected genes on lifespan in Drosophila melanogaster may be experimental artefacts. Credible experiments on the phenotypic effects of transgenesis need proper controls for the effects of insert size and position and should estimate the magnitudes of interactions of treatment with genetic backgrounds.
authors.affiliation: Zoology Institute, University of Basle, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Kaiser
authors.forename: M
authors.initials: M
authors.lastname: Gasser
authors.forename: R
authors.initials: R
authors.lastname: Ackermann
authors.forename: S C
authors.initials: SC
authors.lastname: Stearns
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D008297:Male; D016254:Mutagenesis, Insertional; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D019076:Transgenes
chemical_list: D004251:DNA Transposable Elements; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors