Interleukin-1 beta induces nuclear factor kappa B in epithelial cells independently of the production of reactive oxygen intermediates.
A large body of work has been devoted to tumor necrosis factor alpha or interleukin-1 beta (IL-1 beta) signaling leading to the activation of the transcription factor nuclear factor-kappa B (NF-kappa B) in various cell types. Several studies have indicated that NF-kappa B activation depends strictly on the production of reactive oxygen intermediates. In this report, we first demonstrated that IL-1 beta is a potent activator of NF-kappa B in various epithelial transformed cell lines (OVCAR-3, SKOV-3, MCF7 A/Z). In these cells, IL-1 beta rapidly induces NF-kappa B through a complete degradation of I kappa B-alpha, while H2O2 activates NF-kappa B with slower kinetics through a partial degradation of I kappa B-alpha, p100 and p105. We showed that IL-1 beta-mediated induction of NF-kappa B in OVCAR-3 and in other epithelial cell lines does not proceed through the production of reactive oxygen intermediates, while the same cytokine activates NF-kappa B in lymphoid cells through the intracellular generation of H2O2. Our study demonstrated that several signaling pathways lead to the activation of NF-kappa B, following IL-1 beta treatment in different cell types.
Bonizzi
G
G
Laboratory of Medical Chemistry/Medical Oncology, University of Liège, Belgium.
Dejardin
E
E
Piret
B
B
Piette
J
J
Merville
M P
MP
Bours
V
V
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D007375:Interleukin-1 / Q000502:physiology*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D051841:Transcription Factor RelB; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D007375:Interleukin-1; D016328:NF-kappa B; D011518:Proto-Oncogene Proteins; C497593:RELB protein, human; D017382:Reactive Oxygen Species; C497594:Relb protein, mouse; D014157:Transcription Factors; D051841:Transcription Factor RelB; D006861:Hydrogen Peroxide