The effects of cortical stimulation, anesthesia and recording site on somatosensory evoked potentials in the rat.
The purpose of this study was to standardize the method of spinal cord monitoring with evoked potentials in the rat. Seventeen male Wistar rats were anesthetized with alpha-chloralose and urethane. Somatosensory evoked potential (SEP) and cerebellar evoked potential (CEP) following sciatic nerve stimulation were mapped at different time points after induction of anesthesia. SEP peaks at latencies of 13-18 ms (P13, N18) were localized to an extremely small area over the sensory cortex. In contrasts, a negative peak of the SEP at 11 ms (N11) and the CEP were widely distributed over the cerebral or cerebellar surface. Anesthesia significantly influenced the cortical components of the SEP. In 10 rats, MEP or posterior fossa evoked potential (PFEP) following stimulation of the sensorimotor or cerebellar cortices respectively, were recorded at T9. Stimulation of different points produced little change on the waveforms of the MEP or PFEP. Successive recordings of MEP and SEP revealed that the P13-N18 complex of the SEP was markedly suppressed after MEP recordings were made. In conclusion, this study identified several factors which alter SEP waveforms in the rat including location of recording, anesthesia and sequence with respect to MEP recording. MEP by stimulation of the same sensory cortex as SEP recordings should not be used for concurrent monitoring, since cortical stimulation will change the waveforms of the SEP.
Koyanagi
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Canadian Paraplegic Association Spinal Cord Injury Research Laboratory, Toronto Hospital, Ontario, Canada.
Tator
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D000758:Anesthesia; D000818:Animals; D001921:Brain / Q000502:physiology*; D001931:Brain Mapping; D004558:Electric Stimulation; D005073:Evoked Potentials, Somatosensory / Q000502:physiology*; D008297:Male; D009132:Muscles / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000502:physiology