title: Hemolysins and plasmid profiles of Vibrio parahaemolyticus.
abstract: Forty clinical isolates of Vibrio parahaemolyticus were studied for the production of the thermostable direct hemolysin (TDH), and the TDH-related hemolysin (TRH) including the respective encoding genes, tdh and trh. The presence of TDH and its encoding genes were found amongst 95% of the strains, whereas the TRH was absent amongst these isolates. Thirty-two isolates were found to be plasmid-free, whereas eight isolates possessed plasmids with sizes ranging from 2.4 > or = 23 kb. Using a DNA probe coding for the homologous region of the tdh and trh, it was found that the tdh genes were present on the chromosomal DNA.
authors.affiliation: Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: J
authors.initials: J
authors.lastname: Vadivelu
authors.forename: S D
authors.initials: SD
authors.lastname: Puthucheary
authors.forename: A
authors.initials: A
authors.lastname: Mitin
authors.forename: C Y
authors.initials: CY
authors.lastname: Wan
authors.forename: B
authors.initials: B
authors.lastname: van Melle
authors.forename: J A
authors.initials: JA
authors.lastname: Puthucheary
mesh_terms: D001431:Bacteriological Techniques; D015342:DNA Probes; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000382:microbiology*; D005759:Gastroenteritis / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000097:blood*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D014735:Vibrio Infections / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D006460:Hemolysin Proteins