Cloning of the cDNA encoding rat homologue of the mismatch repair gene MSH2 and its expression during spermatogenesis.
A rat cDNA clone encoding the mismatch repair protein MSH2 has been isolated and characterized. The cDNA has an open reading frame of 2802 nucleotides in length coding for a protein of 933 amino acids (100 kDa). It shows significant homology to human and mouse MSH2. Northern blot analysis of rat MSH2 in the testes of rats of different ages showed maximum expression at 20 days, at which time the germ cells are undergoing premeiotic DNA replication. We observed down-regulation in the expression of rat MSH2 beyond 25 days by which time the germ cells have entered meiotic prophase.
Vani
R G
RG
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Rao
M R
MR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051718:MutS Homolog 2 Protein; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D051718:MutS Homolog 2 Protein