title: Analysis of HLA-DRB1 alleles using PCR-RFLP and PCR-MPH.
abstract: In this study we compare the results of HLA-DRB1 genotyping by PCR-RFLP and PCR-MPH. HLA-DR specificities were also performed by LCT. Samples were obtained from 20 Thai patients who were on the waiting list for kidney transplant. DNA was extracted by phenol-chloroform extraction. It was found that the results gave complete agreement with two methods of DNA typing, however, there were 3 discrepancies in assigning serologic DR specificities and DNA subtypes (p = 0.0001) which were due to the cross reactive antibodies and the lack of potent antisera to define proper HLA-DR subtypes by LCT. These PCR techniques can be applied to identify other alleles such as HLA-DPB1 and HLA-DQB1 which will improve the standard histocompatibility testing in the future.
authors.affiliation: Department of Pathology, Pramongkutklao College of Medicine, Bangkok, Thailand.
authors.forename: O N
authors.initials: ON
authors.lastname: Thalang
authors.forename: C
authors.initials: C
authors.lastname: Sukha
authors.forename: C
authors.initials: C
authors.lastname: Suwanasophon
authors.forename: R
authors.initials: R
authors.lastname: Maneemaroj
authors.forename: Y
authors.initials: Y
authors.lastname: Okubo
authors.forename: N
authors.initials: N
authors.lastname: Tatsumi
mesh_terms: D000483:Alleles; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013785:Thailand
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains