title: Results of microsurgical epididymal sperm aspiration (MESA) ans testicular sperm extraction (TESE) in azoospermic men using intracytoplasmic sperm injection (ICSI).
abstract: The rationale and results of using epididymal and testicular spermatozoa with intracytoplasmic sperm injection (ICSI) for zoospermic patients are reviewed. A total of 128 consecutive ICSI/MESA cycles and a total of 120 consecutive ICSI/TESE cycles were performed up to December 1994. The two-pronuclei fertilization rate per intact oocyte (observed after the injection) was 58% and 60%, respectively, when epididymal and testicular spermatozoa were used. The embryo transfer rate was similar for the two procedures (91% after ICSI/MESA and 90% after ICSI/TESE). Fifty women became pregnant (positive HCG) when epididymal spermatozoa were used (39% per cycle and 40% per embryo transfer). These results are comparable to those obtained when ejaculated spermatozoa are used.
authors.affiliation: Centre for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Ubaldi
authors.forename: M
authors.initials: M
authors.lastname: Camus
authors.forename: H
authors.initials: H
authors.lastname: Tournaye
authors.forename: K
authors.initials: K
authors.lastname: Clasen
authors.forename: Z
authors.initials: Z
authors.lastname: Nagy
authors.forename: J
authors.initials: J
authors.lastname: Smitz
authors.forename: A
authors.initials: A
authors.lastname: Van Steirteghem
authors.forename: P
authors.initials: P
authors.lastname: Devroey
mesh_terms: D002469:Cell Separation; D004822:Epididymis / Q000166:cytology / Q000601:surgery*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D001239:Inhalation; D008297:Male; D008866:Microsurgery; D009845:Oligospermia / Q000601:surgery*; D011247:Pregnancy; D013081:Sperm Motility; D013094:Spermatozoa; D013737:Testis / Q000166:cytology*