[Comparison of the results of determining antibiotic sensitivity on AGV medium and on Mueller-Hinton and isosensitest agars].
To evaluate the adequacy of AGV agar for antimicrobial susceptibility testing, the susceptibility of a range of bacteria to 10 antimicrobials on AGV, Mueller-Hinton and isoSensitest agars, all supplemented with 5 per cent lyzed horse blood was determined. Disc tests were used. In general, AGV agar gave identical susceptibility results to Mueller-Hinton and isoSensitest agars for common gram positive and gram negative bacteria with most of the tested microbials excluding sulphonamides and trimethoprim. With those latter antimicrobials inhibition zones for susceptible organisms were not formed on AGV agar whereas large zones were present on Mueller-Hinton and isoSensitest agars. This discrepancy probably can be explained by the presence of high levels of thymidine in AGV agar; too high to be corrected even by the addition of 5 per cent lysed horse blood. AGV agar is possible to use for susceptibility testing with most of the microbials excluding trimethoprim and sulphonamides.
Kozlov
R S
RS
London Hospital Medical College, UK.
Strachunskiĭ
L S
LS
Livermor
D M
DM
Stetsiuk
O U
OU
Shavrikova
E P
EP
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004058:Diffusion; D005069:Evaluation Studies as Topic; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D003470:Culture Media; D000362:Agar