title: Molecular follow-up of a preinvasive bronchial lesion treated by 13-cis-retinoic acid.
abstract: We report the result of the follow-up molecular analysis of a bronchial carcinoma in situ treated by 13-cis-retinoic acid, which relapsed 9 months after cessation of drug therapy. Loss of heterozygosity at 3p21 and 9p22 genomic sequences were assessed by polymerase chain reaction (PCR) after microdissection of the dysplastic epithelia. Despite a transient regression of the lesion to a lower grade with treatment, molecular analysis showed the persistence of a 3p and 9p deletion in all the bronchial biopsies taken in the same area during a 1 year follow-up, preceding by 9 months the recurrence of the carcinoma in situ. Our findings suggest that molecular follow-up analysis can help to assess the persistence of a malignant clone within a bronchial epithelium that displays a more benign phenotype under retinoid treatment on follow-up. Molecular analysis may be of great importance to evaluate the effects of chemoprevention and to determine the duration of such intervention in responder patients.
authors.affiliation: Institut National de la Santé et de la Recherche Medicale (Unite 295),Faculté de Medecine et de Pharmacie de Rouen, France.
authors.forename: L
authors.initials: L
authors.lastname: Thiberville
authors.forename: P
authors.initials: P
authors.lastname: Payne
authors.forename: J
authors.initials: J
authors.lastname: Metayer
authors.forename: J
authors.initials: J
authors.lastname: Vielkinds
authors.forename: J
authors.initials: J
authors.lastname: LeRiche
authors.forename: B
authors.initials: B
authors.lastname: Palcic
authors.forename: S
authors.initials: S
authors.lastname: Lam
mesh_terms: D001984:Bronchial Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D002278:Carcinoma in Situ / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005500:Follow-Up Studies; D006801:Humans; D015474:Isotretinoin / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness
chemical_list: D004273:DNA, Neoplasm; D015474:Isotretinoin