Methanoplanus petrolearius sp. nov., a novel methanogenic bacterium from an oil-producing well.
A disc-shaped methanogenic bacterium designated strain SEBR 4847T (T = type strain) was isolated from a sample collected from an African offshore oil field. Strain SEBR 4847T was non-motile, had a G + C content of 50 mol% and produced methane from H2 + CO2, formate, and CO2 + propanol. Strain SEBR 4847T grew optimally at 37 degrees C; no growth was observed at 25 degrees C or 45 degrees C. It grew in the presence of up to 50 g/l NaCl; 10-30 g/l was required for optimal growth. The optimum pH for growth was 7.0. Doubling time was about 10 h under optimal conditions. Based on 16S rRNA sequence analysis, the isolate was identified as a new species of the genus Methanoplanus and designated Methanoplanus petrolearius sp. nov. The type strain is SEBR 4847T (= OCM 486).
Ollivier
B
B
Laboratoire ORSTOM de Microbiologie des Anaérobies, Université de Provence, CESB-ESIL, Marseille, France.
Cayol
J L
JL
Patel
B K
BK
Magot
M
M
Fardeau
M L
ML
Garcia
J L
JL
D001431:Bacteriological Techniques; D002965:Classification; D003470:Culture Media; D003596:Cytosine; D004269:DNA, Bacterial / Q000032:analysis; D019605:Euryarchaeota / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification*; D006147:Guanine; D016701:Guinea; D008969:Molecular Sequence Data; D009821:Oils; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017423:Sequence Analysis, RNA; D012988:Soil Microbiology
D003470:Culture Media; D004269:DNA, Bacterial; D009821:Oils; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D006147:Guanine; D003596:Cytosine