title: Allogeneic peripheral blood progenitor cell (PBPC) transplantation in children with haematological malignancies.
abstract: We report on five children with haematological malignancies who underwent allogeneic peripheral blood progenitor cell (PBPC) transplantation. PBPC were harvested from HLA-identical sibling donors after G-CSF (10 micrograms/kg/d s.c.) mobilization. Aphereses were carried out on day 5 after G-CSF using a Cobe Spectra blood cell separator. All PBPC allografts were cryopreserved before transplantation. The median of CD34+ cells and CD3+ cells infused were 14.1 x 10(6)/kg recipient body weight (range 4.92-22.3) and 2.40 x 10(8)/kg recipient body weight (range 0.54 4.82), respectively. Engraftment occurred in all cases. The median time to a neutrophil count > 0.5 x 10(9)/l and a platelet count > 20 x 10(9)/l were 15 and 14 d, respectively. The incidence of severe acute graft-versus-host disease was 20%. These data suggest that allogeneic PBPC transplantation might be an alternative to bone marrow transplantation in children.
authors.affiliation: Department of Paediatric Haematology and Oncology, Hospital Niño Jesús, Autonomous University of Madrid, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Diaz
authors.forename: A
authors.initials: A
authors.lastname: Alegre
authors.forename: M
authors.initials: M
authors.lastname: Villa
authors.forename: A
authors.initials: A
authors.lastname: Benito
authors.forename: M R
authors.initials: MR
authors.lastname: Bernardo
authors.forename: M
authors.initials: M
authors.lastname: Lopez-Botet
authors.forename: L
authors.initials: L
authors.lastname: Madero
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D006086:Graft vs Host Disease / Q000209:etiology; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D007223:Infant; D007937:Leukapheresis; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy*; D015477:Leukemia, Myelomonocytic, Chronic / Q000628:therapy*; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy*; D014184:Transplantation, Homologous; D016896:Treatment Outcome