Monoclonal antibody detection of Pseudomonas spp. in refrigerated meat by an indirect ELISA.
Monoclonal antibodies generated against live cells of Pseudomonas fluorescens have been used in an indirect ELISA format for the detection of Pseudomonas spp. in refrigerated meat. The detection threshold for the ELISA assay developed in this work was 10(4) cfu cm-2.
Gutierrez
R
R
Departamento de Nutrición y Bromatologia III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
García
T
T
González
I
I
Sanz
B
B
Hernández
P E
PE
Martín
R
R
D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005506:Food Contamination; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D011549:Pseudomonas / Q000276:immunology* / Q000302:isolation & purification; D011551:Pseudomonas fluorescens / Q000276:immunology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal