title: Detection and identification of microcystins in the drinking water of Haimen City, China.
abstract: Cyanobacterial toxins, microcystins, have a potent tumor-promoting activity. We investigated the level of microcystins in drinking water collected from 1992 to 1994 in Haimen City, China, where people who drink pond ditch water usually incurred a high incidence rate of hepatocellular carcinoma compared with those who drink well water. High-performance liquid chromatography, liquid chromatography/mass spectrometry (LC/MS), and protein phosphatase inhibition assay (pp assay) were used to identify and quantify the microcystins. Microcystin LR and [D-Asp3]microcystin LR were detected in 2 of 50 samples at a concentration less than 100 ng/L by LC/MS in 1992. Although no microcystins were found by the chemical method in 1993, 6 of 7 samples except for 3 tap water samples showed an approximate amount of 100 ng/L by using the pp assay in 1994. The obtained results supported the epidemiological results reported by Yu.
authors.affiliation: Faculty of Pharmacy, Meijo University, Tempaku, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Harada
authors.forename: M
authors.initials: M
authors.lastname: Oshikata
authors.forename: H
authors.initials: H
authors.lastname: Uchida
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: F
authors.initials: F
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Ueno
authors.forename: S Z
authors.initials: SZ
authors.lastname: Yu
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: G C
authors.initials: GC
authors.lastname: Chen
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000458:Cyanobacteria / Q000737:chemistry; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D014881:Water Supply / Q000032:analysis*
chemical_list: D001427:Bacterial Toxins; D010749:Phosphoprotein Phosphatases