title: Effect of nutrients on alginate synthesis in Azotobacter vinelandii and characterization of the produced alginate.
abstract: The role of nutrients on alginate production by Azotobacter vinelandii was studied in batch cultures. The largest amount of bacterial alginate was obtained in presence of: 0.3 g/l MgSO4.7H2O. 0.4 g/l NaCl, 42 mg/l CaCl2.2H2O,.4 mg/l KH2PO4, 16 mg/l K2HPO4, 2.5 mg/l FeSO4.7H2O, 2.9 mg/l H3BO3, 2 mg/l ZnSO4.7H2O, 2 mg/l Na2MoO4.2H2O, 0.3 mg/l CuSO4.5H2O, 0.2 mg/l MnCl2.4H2O. Alginate production was not enhanced by natural additives or inducing agents, except for acetate, which increased alginate yield. The pure alginate contained 0.36% ash and 0.4% protein. It is similar to algal alginate, but it has an extra acetyl group. It contains 69.5% M-M block, 27.5% M-G block and 3% G-G block.
authors.affiliation: Department of Botany and Microbiology, Faculty of Science, Alexandria University, Egypt.
authors.forename: S A
authors.initials: SA
authors.lastname: Sabry
authors.forename: K M
authors.initials: KM
authors.lastname: Ghanem
authors.forename: W A
authors.initials: WA
authors.lastname: Sabra
mesh_terms: D000464:Alginates / Q000032:analysis / Q000378:metabolism*; D016948:Azotobacter vinelandii / Q000378:metabolism*; D003470:Culture Media; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D018977:Micronutrients / Q000494:pharmacology; D012492:Salts / Q000494:pharmacology
chemical_list: D000464:Alginates; D003470:Culture Media; D006603:Hexuronic Acids; D018977:Micronutrients; D012492:Salts; D020723:Glucuronic Acid