Lethality induced by stannous chloride on Escherichia coli AB1157: participation of reactive oxygen species.
Stannous chloride (SnCl2) has been widely used in nuclear medicine as a reducing agent of pharmaceutical products radiolabelled with technetium-99m. To verify whether the lethality induced by this salt could be mediated by reactive oxygen species (ROS), Escherichia coli cultures were treated with SnCl2 in the presence of catalase, ROS scavengers or metal-ion chelators. The inactivation effect, as measured by survival determination, was abolished by thiourea, sodium benzoate, dipyridyl or catalase. The results suggest the participation of ROS, generated by a Fenton-like reaction, in the lethal effect induced by SnCl2.
Dantas
F J
FJ
UERJ, Inst de Biologia, Dept de Biofisica e Biometria, Rio de Janeiro, Brazil.
Moraes
M O
MO
Carvalho
E F
EF
Valsa
J O
JO
Bernardo-Filho
M
M
Caldeira-de-Araújo
A
A
D015082:2,2'-Dipyridyl / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D019817:Benzoic Acid; D002374:Catalase / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D003470:Culture Media; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D007202:Indicators and Reagents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013890:Thiourea / Q000494:pharmacology; D017971:Tin Compounds / Q000633:toxicity*
D001565:Benzoates; D002614:Chelating Agents; D003470:Culture Media; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D017971:Tin Compounds; C023599:stannous chloride; D015082:2,2'-Dipyridyl; D019817:Benzoic Acid; D002374:Catalase; D013890:Thiourea