Nucleic acid scanning-by-hybridization of enterohemorrhagic Escherichia coli isolates using oligodeoxynucleotide arrays.
Nucleic acid scanning by hybridization (NASBH) is a non-electrophoretic typing strategy that uses gridded oligonucleotides to reproducibly characterize arbitrarily amplified nucleic acid sequences. Membrane-bound arrays of terminally-degenerate oligonucleotides were hybridized to DNA amplification fingerprinting (DAF) products from enterohemorrhagic Escherichia coli O157:H7 isolates. Numerical and cluster analysis of 64 isolates, selected by DAF to represent a single dominant amplification type identified 14 hybridization types. Results show that NASBH is a powerful alternative for the identification of closely related bacteria, can be used successfully in epidemiological studies, and holds potential in general nucleic acid diagnostics.
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Digital Diagnostica S.R.L., Maldonado, Uruguay.
Caetano-Anollés
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D016172:DNA Fingerprinting; D004269:DNA, Bacterial; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D015203:Reproducibility of Results
D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides