title: Recombinant adeno-associated virus mediates a high level of gene transfer but less efficient integration in the K562 human hematopoietic cell line.
abstract: We tested the ability of a recombinant adeno-associated virus (rAAV) vector to express and integrate exogenous DNA into human hematopoietic cells in the absence of selection. We developed an rAAV vector, AAV-tNGFR, carrying a truncated rat nerve growth factor receptor (tNGFR) cDNA as a cell surface reporter under the control of the Moloney murine leukemia virus (MoMuLV) long terminal repeat. An analogous MoMuLV-based retroviral vector (L-tNGFR) was used in parallel, and gene transfer and expression in human hematopoietic cells were assessed by flow cytometry and DNA analyses. Following gene transfer into K562 cells with AAV-tNGFR at a multiplicity of infection (MOI) of 13 infectious units (IU), 26 to 38% of cells expressed tNGFR on the surface early after transduction, but the proportion of tNGFR expressing cells steadily declined to 3.0 to 3.5% over 1 month of culture. At an MOI of 130 IU, nearly all cells expressed tNGFR immediately posttransduction, but the proportion of cells expressing tNGFR declined to 62% over 2 months of culture. The decline in the proportion of AAV-tNGFR-expressing cells was associated with ongoing losses of vector genomes. In contrast, K562 cells transduced with the retroviral vector L-tNGFR expressed tNGFR in a constant fraction. Integration analyses on clones showed that integration occurred at different sites. Integration frequencies were estimated at about 49% at an MOI of 130 and 2% at an MOI of 1.3. Transduction of primary human CD34+ progenitor cells by AAV-tNGFR was less efficient than with K562 cells and showed a declining percentage of cells expressing tNGFR over 2 weeks of culture. Thus, purified rAAV caused very high gene transfer and expression in human hematopoietic cells early after transduction, which steadily declined during cell passage in the absence of selection. Although the efficiency of integration was low, overall integration was markedly improved at a high MOI. While prolonged episomal persistence may be adequate for gene therapy of nondividing cells, a very high MOI or improvements in basic aspects of AAV-based vectors may be necessary to improve integration frequency in the rapidly dividing hematopoietic cell population.
authors.affiliation: Division of Research Immunology/Bone Marrow Transplantation, Childrens Hospital Los Angeles, University of Southern California School of Medicine, USA.
authors.forename: P
authors.initials: P
authors.lastname: Malik
authors.forename: S A
authors.initials: SA
authors.lastname: McQuiston
authors.forename: X J
authors.initials: XJ
authors.lastname: Yu
authors.forename: K A
authors.initials: KA
authors.lastname: Pepper
authors.forename: W J
authors.initials: WJ
authors.lastname: Krall
authors.forename: G M
authors.initials: GM
authors.lastname: Podsakoff
authors.forename: G J
authors.initials: GJ
authors.lastname: Kurtzman
authors.forename: D B
authors.initials: DB
authors.lastname: Kohn
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D051381:Rats; D017475:Receptors, Nerve Growth Factor / Q000235:genetics*; D011995:Recombination, Genetic; D012641:Selection, Genetic; D014407:Tumor Cells, Cultured; D016662:Virus Integration
chemical_list: D018952:Antigens, CD34; D017475:Receptors, Nerve Growth Factor; D004247:DNA