The glycosylation of the influenza A virus hemagglutinin by mammalian cells. A site-specific study.
We have characterized the glycans at individual sites on the hemagglutinin of three influenza A variants to obtain information on the role of cell-specific glycosylation in determining the receptor binding properties of this virus. The variants differ in whether they have a glycosylation site at residue 129 on the tip of the hemagglutinin and whether amino acid 184 (near to the receptor binding site) is His or Asn. We found that all sites on each variant are glycosylated in Madin-Darby bovine kidney cells, that the glycosylation is site-specific, and that the glycans at the same site in each variant are highly similar. One site that is buried in the hemagglutinin trimer contains only oligomannose glycans. The remaining sites carry complex glycans of increasing size as the distance of the site from the viral membrane decreases. Most of these complex glycans are terminated with alpha-galactose residues, a consequence in bovine cells of the removal of terminal sialic acids by the viral neuraminidase. Although the glycans at residue 129 are among the smallest on the molecule, they are large enough to reach the receptor binding pocket on their own and adjacent monomers. The results suggest that the reduction in receptor binding observed with Madin-Darby bovine kidney cell-grown virus is due to the combined effect of large complex glycans at the tip of the hemagglutinin and a His to Asn substitution close to the receptor binding pocket.
Mir-Shekari
S Y
SY
Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks, Oxford OX1 3QU, United Kingdom.
Ashford
D A
DA
Harvey
D J
DJ
Dwek
R A
RA
Schulze
I T
IT
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002417:Cattle; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006026:Glycoside Hydrolases