Quantitative nucleic acids footprinting: thermodynamic and kinetic approaches.
Quantitative footprinting techniques allow a detailed analysis of the thermodynamic forces that characterize nucleic acid-ligand interactions and ligand-induced changes in nucleic acid structure by separately resolving the intrinsic and cooperative Gibbs free energy changes describing the reactions being investigated. A new implementation of the quantitative footprinting technique is the application of stopped-flow techniques to the study of kinetic reactions.
Petri
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V
Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
Brenowitz
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M
D001665:Binding Sites; D001709:Biotechnology; D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting / Q000379:methods*; D003850:Deoxyribonuclease I; D007700:Kinetics; D008024:Ligands; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics
D008024:Ligands; D012313:RNA; D004247:DNA; D003850:Deoxyribonuclease I