Design and production of novel tetravalent bispecific antibodies.
We have produced novel bispecific antibodies by fusing the DNA encoding a single chain antibody (ScFv) after the C terminus (CH3-ScFv) or after the hinge (Hinge-ScFv) with an antibody of a different specificity. The fusion protein is expressed by gene transfection in the context of a murine variable region. Transfectomas secrete a homogeneous population of the recombinant antibody with two different specificities, one at the N terminus (anti-dextran) and one at the C terminus (anti-dansyl). The CH3-ScFv antibody, which maintains the constant region of human IgG3, has some of the associated effector functions such as long half-life and Fc receptor binding. The Hinge-ScFv antibody which lacks the CH2 and CH3 domains has no known effector functions.
Coloma
M J
MJ
Department of Microbiology and Molecular Genetics, University of California at Los Angeles 90095, USA.
Morrison
S L
SL
D000818:Animals; D018033:Antibodies, Bispecific / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D000915:Antibody Affinity; D002850:Chromatography, Gel; D003619:Dansyl Compounds; D015195:Drug Design; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000096:biosynthesis* / Q000737:chemistry; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000737:chemistry; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007700:Kinetics; D008024:Ligands; D051379:Mice; D009101:Multiple Myeloma; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D014162:Transfection
D018033:Antibodies, Bispecific; D003619:Dansyl Compounds; D007127:Immunoglobulin Constant Regions; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D008024:Ligands; D011993:Recombinant Fusion Proteins