title: Dipodascus magnusii (Saccharomycetes) contains multiple glucose-6-phosphate dehydrogenases with different NAD+/NADP+ dependencies.
abstract: A cell-free extract of a morphologically unstable strain of Dipodascus magnusii contained six proteins with activity of glucose-6-phosphate dehydrogenase (G6PDH). Two of these proteins displayed only NADP(+)-dependent activity, two could utilize both NAD+ and NADP+, but had higher activity with NAD+, and two possessed only NAD(+)-dependent activity. When the cultivation was carried out in the presence of monoiodoacetic acid, only two proteins with G6PDH activity were produced, one of them NAD(+)-dependent and the other NADP(+)-dependent. In all cases, NAD(+)-dependent activity was less stable in the presence of proteinases than was the NADP(+)-dependent activity.
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic. blanka.kralova@vscht.cz
authors.forename: B
authors.initials: B
authors.lastname: Králová
authors.forename: O
authors.initials: O
authors.lastname: Valentová
authors.forename: K
authors.initials: K
authors.lastname: Demnerová
authors.forename: L
authors.initials: L
authors.lastname: Silhánková
mesh_terms: D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D004718:Saccharomycetales / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D007527:Isoenzymes; D009243:NAD; D009249:NADP; D005954:Glucosephosphate Dehydrogenase