Influence of glycoproteins B, C and D on the conversion of virus-to-cell attachment from heparin sensitivity to resistance.
Glycoprotein C-negative (gC-) mutants of herpes simplex virus type 1 (HSV-1) derived from strains KOS and ANGpath were used to analyse the influence of soluble heparin on the phase of adsorption/attachment of HSV-1 to cells. A dose of 200 micrograms/ml heparin given 20 mins after infection of cells with the gC- positive (gC+) strains KOS and ANGpath at 4 degrees C reduced the adsorption of infective particles to 20-30% of the controls. A weaker heparin effect was observed with gC- mutants. However, also the gC- mutants exhibited a short heparin-sensitive phase. Mutations in amino acids of gB or gD at positions 854 or 25 and 27, respectively, did not alter the attachment capacities of these HSV mutants in the presence of heparin despite their peculiar fusion properties and resistance to soluble gD. We conclude that HSV-1 strains exhibit a heparin-resistant phase of attachment, which is determined by gC. Lack of gC delays the heparin-resistant attachment phase of HSV-1 to cells.
Seck
T
T
Institute of Virology, Johannes Gutenberg University, Mainz, Germany.
Koch
O
O
Lingen
M
M
Falke
D
D
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004352:Drug Resistance, Microbial; D006493:Heparin / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D016296:Mutagenesis; D011189:Potassium Chloride / Q000494:pharmacology; D013696:Temperature; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C039447:glycoprotein D, Human herpesvirus 1; C038535:glycoprotein gC, herpes simplex virus type 1; D011189:Potassium Chloride; D006493:Heparin