Highly purified CD25- resting T cells cannot be infected de novo with HIV-1.
Previous studies have demonstrated that the expression of CD25 can distinguish CD25- latently infected cells from CD25+ cells actively producing virus. Our studies were designed to characterize the nature and stability of the viral genome in CD25- quiescent HIV-1-infected cells and to determine whether these cells could be infected de novo with HIV-1. Our results show that: (i) When unfractionated peripheral blood mononuclear cells are first infected with HIV-1 and the CD25- cells then isolated, the latter contain only incomplete DNA transcripts and no full-length DNA or 2-LTR circles. Phytohemagglutinin activation of these CD25- cells results in the generation of full-length viral DNA and p24 production. (ii) When CD25- CD4+ cells are first purified from peripheral blood mononuclear cells and then incubated with HIV-1, viral DNA cannot be detected, suggesting that these purified cells cannot be infected. Furthermore, CD25-adherent cells do not facilitate the infection of CD4+ CD25- T cells when they were present at the time of incubation with HIV-1. Taken together, these studies suggest either that (i) the CD25- cells containing incomplete DNA transcripts are derived from infected-activated CD25+ cells, which subsequently become CD25- or (ii) the presence of CD25+ cells is required for the infection of CD25- cells in vitro.
Chou
C S
CS
Department of Microbiology, The University of Texas Southwestern Medical Center, Dallas 75235, USA.
Ramilo
O
O
Vitetta
E S
ES
D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002448:Cell Adhesion; D002469:Cell Separation; D004279:DNA, Viral / Q000096:biosynthesis; D016679:Genome, Viral; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000254:growth & development*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008213:Lymphocyte Activation; D015375:Receptors, Interleukin-2; D013601:T-Lymphocytes / Q000821:virology*; D017735:Virus Latency
D004279:DNA, Viral; D016655:HIV Core Protein p24; D015375:Receptors, Interleukin-2