Antiviral activity of crude extracts of Guarea guidona.
Crude extracts of leaves and fruits of Guarea guidona were tested for antiviral activity against pseudorabies virus and foot-and-mouth disease virus in the IB-RS-2 pig cell line and against bovine herpesvirus-1 (BHV-1) in the GBK bovine cell line. The highest nontoxic doses of extracts from fruits and leaves were 125 micrograms/ml and 500 micrograms/ml. respectively. Crude extracts presented antiviral activity against pseudorabies virus with a decrease in virus titer of 3.0 log units at 500 micrograms/ml. Virucidal activity was not observed at 62.5 micrograms/ml. Preformed cell monolayers showed no cytotoxic effect after 48 h in the presence of 500 micrograms/ml in pig cells. G. guidona leaves did not induce an antiviral state but exhibited antiviral effects during the early stage of viral infection.
Simoni
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Seção ce Biologia Celular, Instituto Biológico, São Paulo, Brasil.
Munford
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Felicio
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Lins
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AP
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D005537:Aphthovirus / Q000187:drug effects*; D002417:Cattle; D002460:Cell Line / Q000187:drug effects; D002478:Cells, Cultured; D007242:Herpesvirus 1, Bovine / Q000187:drug effects*; D011558:Herpesvirus 1, Suid / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D013552:Swine
D000998:Antiviral Agents; D010936:Plant Extracts