Increase in lysophosphatidylethanolamine in the cell membrane upon the regulated exocytosis of pancreatic acinar AR42J cells.
A specific cytosolic phospholipase A2 inhibitor, arachidonyl trifluoromethyl ketone (AACOCF3), was found to inhibit the regulated exocytosis of pancreatic acinar AR42J cells. When AR42J cells were stimulated with cholecystokinin octapeptide (CCK-8), the regulated exocytosis monitored by amylase release was rapidly activated and increased by 2.5-fold during one hour. After AR42J cells were treated with AACOCF3, amylase release by CCK-8 remained at the basal level. Thus, changes in the composition of membrane phospholipids before and after stimulation were investigated. Within 1 min after CCK-8 stimulation, lysophosphatidylethanolamine (lysoPE) in the cellular membranes of AR42J was increased while lysophosphatidylcholine stayed unchanged. In the presence of AACOCF3, lysoPE was not increased by CCK-8. Those results indicate that the increment of lysoPE is linked to the regulated exocytosis.
Ikeda
Y
Y
Research Institute for Food Science, Kyoto University, Japan.
Fukuoka
S
S
Kito
M
M
D000681:Amylases / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D008246:Lysophospholipids / Q000737:chemistry / Q000378:metabolism*; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D010179:Pancreas / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010741:Phospholipases A / Q000187:drug effects / Q000378:metabolism; D054467:Phospholipases A2; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D012844:Sincalide / Q000494:pharmacology
D001095:Arachidonic Acids; D004791:Enzyme Inhibitors; D008246:Lysophospholipids; D008563:Membrane Lipids; D010743:Phospholipids; C008301:lysophosphatidylethanolamine; C081565:arachidonyltrifluoromethane; D003907:Dexamethasone; D010741:Phospholipases A; D054467:Phospholipases A2; D000681:Amylases; D012844:Sincalide