False positives from the yeast two-hybrid system.
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methods-reagents, available on the Internet. This month's column discusses false positives found when using the yeast two-hybrid system to isolate interactive proteins. For details on how to partake in the newsgroup, see the accompanying box.
Hengen
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PN
National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA. pnh@ncifcrf.gov
D005978:Glutathione / Q000096:biosynthesis / Q000235:genetics; D006824:Hybridization, Genetic / Q000235:genetics*; D011237:Predictive Value of Tests; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D005978:Glutathione