Metanestin, a glycoprotein with metastasis-associated expression in transitional cell carcinoma of the urinary bladder.
A 60-kDa glycoprotein named metanestin was identified by molecular cloning. The glycoprotein had a twice-repeated motif of Pro-Gly-Pro-Gly and carried metastasis-associated carbohydrate epitopes. The antibody to the protein portion of metanestin strongly reacted with lymph-node metastasis of transitional-cell carcinoma of the urinary bladder, but the reactivity to the primary tumor was generally weaker and the normal urothelium was scarcely reactive. Thus both metanestin and the carbohydrate on it showed metastasis-associated expression. In addition, we identified a 100-kDa glycoprotein with a 4-times-repeated motif of Pro-Ala-Pro-Ala. The antibody to the 100-kDa glycoprotein showed reactivity similar to that to metanestin.
Takemoto
M
M
Department of Urology, Faculty of Medicine, Kagoshima University, Japan.
Shirahama
T
T
Miyauchi
T
T
Matsusako
T
T
Kaneda
N
N
Muramatsu
H
H
Ozawa
M
M
Ohi
Y
Y
Muramatsu
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D002295:Carcinoma, Transitional Cell / Q000378:metabolism / Q000473:pathology*; D002460:Cell Line; D003001:Cloning, Molecular; D000939:Epitopes / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D008207:Lymphatic Metastasis; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*
D014408:Biomarkers, Tumor; D000939:Epitopes; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D011993:Recombinant Fusion Proteins; C104590:metanestin protein, human