Effect of gene therapy with the herpes simplex virus-thymidine kinase gene on hepatic metastasis in murine colon cancer.
Hepatic metastasis of colon cancer is an important prognostic factor for survival. In this study, we examined the effect of gene therapy using the herpes simplex virus-thymidine kinase (HS-tk) gene with short-course ganciclovir (GCV) treatment for multiple hepatic metastases of murine colon cancer. Colon26 cells transfected with the HS-tk gene were found to be sensitive to GCV in a concentration-dependent way. On the other hand, induction of the HS-tk gene in the cells had no influence on cell growth in vitro. However, multiple hepatic metastases of Colon26 cells transfected with HS-tk gene were significantly suppressed by the GCV treatment. These results thus suggest that HS-tk gene therapy is useful for the treatment of hepatic metastasis in colon cancer.
Hayashi
S
S
Second Department of Surgery, Nagoya University School of Medicine, Japan.
Emi
N
N
Yokoyama
I
I
Uchida
K
K
Takagi
H
H
D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology / Q000628:therapy*; D005260:Female; D015774:Ganciclovir / Q000627:therapeutic use*; D015316:Genetic Therapy; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000556:secondary* / Q000628:therapy; D051379:Mice; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D013937:Thymidine Kinase; D015774:Ganciclovir