title: Two amino acid amidohydrolase genes encoding L-stereospecific carbamoylase and aminoacylase are organized in a common operon in Bacillus stearothermophilus.
abstract: The L-carbamoylase gene (amaB) upstream of the previously detected L-aminoacylase gene (amaA) in the Bacillus stearothermophilus NCIB8224 strain was identified in this study. The amaB and amaA genes are cotranscribed as a single mRNA from the same transcriptional start. The two-ama-gene operon is conserved in B. stearothermophilus strains. A cross-activity of L-carbamoylase towards respective substrates for L-aminoacylase supports the hypothesis of a common ancestor for both amino acid amidohydrolase genes.
authors.affiliation: Laboratoire de Biotechnologie, Faculté des Sciences et des Techniques, Université de Nantes, France.
authors.forename: N
authors.initials: N
authors.lastname: Batisse
authors.forename: P
authors.initials: P
authors.lastname: Weigel
authors.forename: M
authors.initials: M
authors.lastname: Lecocq
authors.forename: V
authors.initials: V
authors.lastname: Sakanyan
mesh_terms: D000581:Amidohydrolases / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D005798:Genes, Bacterial; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D000581:Amidohydrolases; C019672:aminoacylase I; C104561:N-carbamoyl-L-amino-acid hydrolase