Detection of DNA damage in stimulated human lymphocytes after adding cytostatic drugs in vitro. A model to demonstrate individual damage rates.
The DNA damaging effect of different concentrations of methotrexate, 6-mercaptopurine, 6-thioguanine and cisplatin was tested by nucleoid sedimentation in pokeweed mitogen (PWM)-stimulated lymphocytes of 16 healthy persons in vitro. The examined persons show an individual variation of DNA damage demonstrating individual differences in DNA repair. The method can be used to identify persons with a low DNA repair capacity, and possible cytogenetic side effects of cytostatic drugs can be calculated before starting a cancer therapy. In clinical practice the use of cytostatic drugs is limited because of the side effects on normal tissues. The cancer therapist can improve a cytostatic therapy when he obtains information about possible DNA damage of cytostatic drugs in cells of the patient at the beginning of the therapy.
Reitz
M
M
Clinic of Anesthesiology, University of Mainz, Germany.
Fritsch
G
G
Löber
G
G
Gutjahr
P
P
D000328:Adult; D000970:Antineoplastic Agents / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000000981:diagnostic imaging / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011859:Radiography; D013268:Stimulation, Chemical
D000970:Antineoplastic Agents; D011043:Pokeweed Mitogens