Trace analysis of albendazole and its sulphoxide and sulphone metabolites in milk by liquid chromatography.
Analytical methodology for determination of albendazole and its sulphoxide and sulphone metabolites in milk at levels down to 2-5 ng/ml has been developed. Extraction was carried out with ethyl acetate under alkaline conditions, and extracts were analyzed on a silica-based C18 column in the presence of positively-charged pairing ions. Accuracy data showed overall recoveries ranged from 78.4% to 100%, whereas precision data, based on within and between-day variation, suggested overall precision values better than 4.9%. The method was successfully applied to determine residues in milk of a dairy cow orally given albendazole.
Fletouris
D J
DJ
Laboratory of Milk Hygiene and Technology, School of Veterinary Medicine, Aristotle University, Thessoloniki, Greece.
Botsoglou
N A
NA
Psomas
I E
IE
Mantis
A I
AI
D015766:Albendazole / Q000031:analogs & derivatives / Q000032:analysis*; D000818:Animals; D000871:Anthelmintics / Q000032:analysis; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D005240:Feasibility Studies; D005260:Female; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013450:Sulfones / Q000032:analysis; D013454:Sulfoxides / Q000032:analysis
D000871:Anthelmintics; D013450:Sulfones; D013454:Sulfoxides; C027187:albendazole sulfone; D015766:Albendazole; C027186:albendazole sulfoxide