Catalysis of protein folding by symmetric chaperone complexes.
The GroE chaperones of Escherichia coli assist protein folding under physiological and heat shock conditions in an ATP-dependent way. Although a number of details of assisted folding have been elucidated, the molecular mechanism of the GroE cycle remains unresolved. Here we present an experimental system that allows the direct analysis of the GroE-mediated folding cycle under stringent conditions. We demonstrate that the GroE proteins efficiently catalyze the folding of kinetically trapped folding intermediates of a mutant of maltose-binding protein (MBP Y283D) in an ATP-dependent way. GroES plays a key role in this reaction cycle, accelerating the folding of the substrate protein MBP Y283D up to 50-fold. Interestingly, catalysis of the folding reaction requires the formation of symmetrical football-shaped GroEL x GroES2 particles and the intermediate release of the nonnative protein from the chaperone complex. Our results show that, in the presence of GroES, the complex architecture of the GroEL toroids allows maintenance of two highly interregulated rings simultaneously active in protein folding.
Sparrer
H
H
Institut für Biophysik & Physikalische Biochemie, Universität Regensburg, Germany.
Rutkat
K
K
Buchner
J
J
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins / Q000378:metabolism* / Q000648:ultrastructure; D002384:Catalysis; D018835:Chaperonin 10 / Q000378:metabolism* / Q000648:ultrastructure; D018834:Chaperonin 60 / Q000378:metabolism* / Q000648:ultrastructure; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins / Q000378:metabolism* / Q000648:ultrastructure; D008954:Models, Biological; D009004:Monosaccharide Transport Proteins; D011485:Protein Binding; D017510:Protein Folding
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; C461599:maltose transport system, E coli