Effect of nutrients on alginate synthesis in Azotobacter vinelandii and characterization of the produced alginate.
The role of nutrients on alginate production by Azotobacter vinelandii was studied in batch cultures. The largest amount of bacterial alginate was obtained in presence of: 0.3 g/l MgSO4.7H2O. 0.4 g/l NaCl, 42 mg/l CaCl2.2H2O,.4 mg/l KH2PO4, 16 mg/l K2HPO4, 2.5 mg/l FeSO4.7H2O, 2.9 mg/l H3BO3, 2 mg/l ZnSO4.7H2O, 2 mg/l Na2MoO4.2H2O, 0.3 mg/l CuSO4.5H2O, 0.2 mg/l MnCl2.4H2O. Alginate production was not enhanced by natural additives or inducing agents, except for acetate, which increased alginate yield. The pure alginate contained 0.36% ash and 0.4% protein. It is similar to algal alginate, but it has an extra acetyl group. It contains 69.5% M-M block, 27.5% M-G block and 3% G-G block.
Sabry
S A
SA
Department of Botany and Microbiology, Faculty of Science, Alexandria University, Egypt.
Ghanem
K M
KM
Sabra
W A
WA
D000464:Alginates / Q000032:analysis / Q000378:metabolism*; D016948:Azotobacter vinelandii / Q000378:metabolism*; D003470:Culture Media; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D018977:Micronutrients / Q000494:pharmacology; D012492:Salts / Q000494:pharmacology
D000464:Alginates; D003470:Culture Media; D006603:Hexuronic Acids; D018977:Micronutrients; D012492:Salts; D020723:Glucuronic Acid