title: [Utilization of different microbiological markers in the study of Haemophilus influenzae].
abstract: The present study includes 178 Haemophilus influenzae strains isolated in different pediatric hospitals from Havana, Cuba, during 1991-1994, associated to divers infections (meningitis, respiratory sepsis, primary bacteremia). A combination of various typing and subtyping methods was used as epidemiological markers: serotyping (slide agglutination with diagnostical serum a-f and latex agglutination), biotyping according to Killian's procedures (by determination of indole production, urease and ornithine decarboxylase activity), subtyping by fermentative profiles according to Roberts' methods (glucose, maltose, xylose and fructose) and outer membrane protein profile subtyping (vesicles extraction by a modified Barenkamp's method, analysis by lineal and gradient SDS-PAGE and assessment according to our own classification system). Serotype b was identified in 89.3%, biotype I was the most frequent (79.1%), other biotypes (II, III, IV and V) were also identified. Fermentative profile D (glucose, maltose, xylose and fructose positive) was the most frequent (52.8%) while profile G (glucose, maltose, xylose positive and fructose negative) represented 20.2%. Other known profiles were present. PA2 (33.7%) was the most frequent OMP subtype. Even though 11 different protein subtypes were found, the 77.5% of the strains were located in only three OMP electrophoretic subtypes (PA2, PC1, LA2).
authors.affiliation: Departamento de Investigaciones Meningeas Bacterianas, Instituto Finlay, La Habana, Cuba.
authors.forename: L E
authors.initials: LE
authors.lastname: Azahares Romero
authors.forename: M
authors.initials: M
authors.lastname: Cuello
authors.forename: A H
authors.initials: AH
authors.lastname: Callis
authors.forename: R
authors.initials: R
authors.lastname: Llanes
authors.forename: L
authors.initials: L
authors.lastname: Estrada
authors.forename: M F
authors.initials: MF
authors.lastname: Pérez
authors.forename: I
authors.initials: I
authors.lastname: Collada
authors.forename: G
authors.initials: G
authors.lastname: Sierra
mesh_terms: D000372:Agglutination Tests; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D015415:Biomarkers; D050260:Carbohydrate Metabolism; D002675:Child, Preschool; D003470:Culture Media / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D006192:Haemophilus Infections / Q000382:microbiology; D006193:Haemophilus influenzae / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn
chemical_list: D001425:Bacterial Outer Membrane Proteins; D015415:Biomarkers; D003470:Culture Media