title: Schistosoma japonicum: immunolocalization of paramyosin during development.
abstract: This paper describes the localization of paramyosin immunoreactivity in Schistosoma japonicum and represents the first comparative immunolocalization study among schistosome adult, cercariae and lung schistosomula by electron microscopy. A polyclonal antibody was utilized to immunolabel paramyosin or paramyosin-like proteins. Paramyosin was localized within the muscle layer of all 3 developmental stages. Furthermore, paramyosin was localized within granules of the post-acetabular glands of cercariae, and within the tegument matrix and surface of lung schistosomules. Adults and cercariae did not display any detectable paramyosin on the surface or within the tegument. The possible functions of paramyosin within S. japonicum and the relevance of these findings in relation to the reported protective properties of paramyosin as an anti-schistosome vaccine target molecule are discussed.
authors.affiliation: Analytical Electron Microscopy Facility, Queensland University of Technology, Brisbane, Australia. g.gobert@qut.edu.au
authors.forename: G N
authors.initials: GN
authors.lastname: Gobert
authors.forename: D J
authors.initials: DJ
authors.lastname: Stenzel
authors.forename: M K
authors.initials: MK
authors.lastname: Jones
authors.forename: D E
authors.initials: DE
authors.lastname: Allen
authors.forename: D P
authors.initials: DP
authors.lastname: McManus
mesh_terms: D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D012549:Schistosoma japonicum / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure; D012554:Schistosomiasis japonica / Q000469:parasitology*; D012908:Snails; D014335:Tropomyosin / Q000032:analysis* / Q000276:immunology / Q000502:physiology
chemical_list: D014335:Tropomyosin