title: Positional cloning of a gene for nematode resistance in sugar beet.
abstract: The Hs1(pro-1) locus confers resistance to the beet cyst nematode (Heterodera schachtii Schmidt), a major pest in the cultivation of sugar beet (Beta vulgaris L.). The Hs1(pro-1) gene was cloned with the use of genome-specific satellite markers and chromosomal break-point analysis. Expression of the corresponding complementary DNA in a susceptible sugar beet conferred resistance to infection with the beet cyst nematode. The native Hs1(pro-1) gene, expressed in roots, encodes a 282-amino acid protein with imperfect leucine-rich repeats and a putative membrane-spanning segment, features similar to those of disease resistance genes previously cloned from higher plants.
authors.affiliation: Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Cai
authors.forename: M
authors.initials: M
authors.lastname: Kleine
authors.forename: S
authors.initials: S
authors.lastname: Kifle
authors.forename: H J
authors.initials: HJ
authors.lastname: Harloff
authors.forename: N N
authors.initials: NN
authors.lastname: Sandal
authors.forename: K A
authors.initials: KA
authors.lastname: Marcker
authors.forename: R M
authors.initials: RM
authors.lastname: Klein-Lankhorst
authors.forename: E M
authors.initials: EM
authors.lastname: Salentijn
authors.forename: W
authors.initials: W
authors.lastname: Lange
authors.forename: W J
authors.initials: WJ
authors.lastname: Stiekema
authors.forename: U
authors.initials: U
authors.lastname: Wyss
authors.forename: F M
authors.initials: FM
authors.lastname: Grundler
authors.forename: C
authors.initials: C
authors.lastname: Jung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D005816:Genetic Complementation Test; D007930:Leucine / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009348:Nematoda / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology; D010940:Plant Proteins; D018517:Plant Roots / Q000235:genetics / Q000469:parasitology; D014170:Transformation, Genetic; D014675:Vegetables / Q000235:genetics* / Q000469:parasitology*
chemical_list: D018076:DNA, Complementary; C103918:Hs1 pro-1 protein, Beta procumbens; D008565:Membrane Proteins; D010940:Plant Proteins; D007930:Leucine