High incidence of a T nucleotide at the second position of codon 97 in Vdelta2-(D)-Jdelta1 junctional sequences of human normal skin gamma delta T-cells.
In this investigation, we have demonstrated that in-frame polyclonal Vdelta2-(D)-Jdelta1 junctional sequences from human skin gammadelta T-cells contain a high incidence of T nucleotides at the second position of codon 97. Analysis of the deduced amino acid sequences based on in-frame Vdelta2-(D)-Jdelta1 junctional nucleotide sequences from normal skin gammadelta T-cells revealed a high incidence of the amino acids valine and leucine at position 97. These results are consistent with the studies in peripheral blood gammadelta T-cells, but not with previous findings in skin gammadelta T-cells, where random nucleotides were observed in the second position of codon 97 of Vdelta2-(D)-Jdelta1 junctional sequences and only a small minority of the deduced Vdelta2-(D)-Jdelta 1 amino acid sequences showed the amino acids valine and leucine at codon 97. Therefore, our findings indicate that the human skin gamma delta T-cells with a T-cell receptor consisting of a Vdelta2-(D)-Jdelta1-C delta1 chain are not a subset distinct from the subpopulation of human peripheral blood gammadelta T-cells expressing the same chain.
Alaibac
M
M
Unit of Dermatology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Chu
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AC
D003062:Codon; D006801:Humans; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012867:Skin / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine
D003062:Codon; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013936:Thymidine