Effect of colchiceine and ursodeoxycholic acid on hepatocyte and erythrocyte membranes and liver histology in experimentally induced carbon tetrachloride cirrhosis in rats.
Colchiceine and ursodeoxycholic acid (UDCA) are drugs currently in use as therapy for different types of liver damage. We evaluated their ability to reverse the damage induced by carbon tetrachloride (CCl4) in rats. Six groups were analysed: (1) CCl4 (0.4 g kg(-1), i.p., three times a week) for 13 weeks; (2) CCl4 for 8 weeks followed by colchiceine (60 microg kg(-1)) + CCl4 for 5 weeks; (3) CCl4 for 8 weeks and thereafter UDCA (25 mg kg(-1)) + CCl4 for 5 weeks. Groups 4, 5 and 6 were appropriate controls of colchiceine, UDCA and vehicles respectively. Na+,K+- and Ca2+-ATPase activities and the cholesterol-phospholipid (CH/PL) ratio from erythrocyte and hepatocyte membranes were quantified. Membrane enzymatic activities and CH/PL ratios were affected more in group 1 than groups 2 and 3. We concluded that colchiceine and UDCA were effective drugs in this model of liver damage.
Nava-Ocampo
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Unidad de Investigacion Medica en Farmacologia, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, IMSS, Mexico.
Suster
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Muriel
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D000818:Animals; D000252:Calcium-Transporting ATPases / Q000097:blood / Q000737:chemistry; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002784:Cholesterol / Q000097:blood / Q000737:chemistry; D003078:Colchicine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D008099:Liver / Q000187:drug effects* / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D010743:Phospholipids / Q000097:blood / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood / Q000737:chemistry; D014580:Ursodeoxycholic Acid / Q000494:pharmacology* / Q000627:therapeutic use
D010743:Phospholipids; D014580:Ursodeoxycholic Acid; D002784:Cholesterol; D002251:Carbon Tetrachloride; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; C012852:colchiceine; D003078:Colchicine