Structural gene (nirS) for the cytochrome cd1 nitrite reductase of Alcaligenes eutrophus H16.
Denitrification by Alcaligenes eutrophus H16 is genetically linked to megaplasmid pHG1. Unexpectedly, the gene encoding the nitrite reductase (nirS) was identified on chromosomal DNA. The nirS product showed extensive homology with periplasmic nitrite reductases of the heme cd1-type. Disruption of nirS abolished nitrite-reducing ability, indicating that NirS is the enzyme essential for denitrification in A.eutrophus.
Rees
E
E
Institut für Biologie, Humboldt-Universität zu Berlin, Germany.
Siddiqui
R A
RA
Köster
F
F
Schneider
B
B
Friedrich
B
B
D000421:Alcaligenes / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D002876:Chromosomes, Bacterial; D003580:Cytochromes; D003576:Electron Transport Complex IV / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009572:Nitrite Reductases / Q000235:genetics*; D009573:Nitrites / Q000378:metabolism*; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D003580:Cytochromes; D009573:Nitrites; D009572:Nitrite Reductases; C028123:nitrite reductase (NO-forming); D003576:Electron Transport Complex IV