Lactoferrin as a suppressor of cell migration of gastrointestinal cell lines.
The effects of lactoferrin (Lf), an iron-binding glycoprotein, on cell migration were investigated. Lf inhibited the cell migration of three gastrointestinal cell lines (Caco-2 cells, AGS cells, and IEC-18 cells) in vitro. Both iron-saturated (holo) and iron-depleted (apo) Lf showed this inhibitory effect. Chelation of iron in the culture medium by desferrioxamine did not affect the activity of either form of Lf. A pepsin hydrolysate of Lf exhibited effectiveness similar to that of intact Lf. These results demonstrate a novel activity of Lf and suggest a potential role for this molecule in gastrointestinal wound healing, which is independent of its iron-binding capacity.
Nakajima
M
M
Nutritional Science Laboratory, Morinaga Milk Industry Company, Ltd., Kanagawa, Japan.
Shinoda
I
I
Samejima
Y
Y
Miyauchi
H
H
Fukuwatari
Y
Y
Hayasawa
H
H
D000230:Adenocarcinoma; D000818:Animals; D001059:Apoproteins / Q000494:pharmacology; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects*; D003110:Colonic Neoplasms; D003676:Deferoxamine / Q000494:pharmacology; D004068:Digestive System Physiological Phenomena; D006801:Humans; D007413:Intestinal Mucosa; D007700:Kinetics; D007781:Lactoferrin / Q000494:pharmacology*; D051381:Rats; D013274:Stomach Neoplasms
D001059:Apoproteins; C039223:apolactoferrin; D007781:Lactoferrin; D003676:Deferoxamine