A 38 bp repeat sequence within the pea seed storage protein promoter of legA is a binding site for a nuclear DNA-binding protein.
This paper describes a target sequence for a pea seed nuclear DNA-binding protein (of Pisum sativum) present at the cell expansion phase. Electromobility shift assays as well as in situ copper-phenanthroline footprinting have shown that this protein binds to an imperfect repeat sequence of the legA promoter located -404 tot -367 relative to the transcriptional start site, a region designated USR1 (-549 tot -316). Competition assays showed that the same or a related protein binds to USR2 located at -833 to -582. Another protein that does not recognize the repeat sequence as a target site binds adjacent but upstream to the repeat sequence in USR1. It is suggested that these protein-DNA interactions are initial events in the assembly of a transcriptional activation complex.
Howley
P M
PM
Biological Sciences Department, University of Durham, UK.
Gatehouse
J A
JA
D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D000097793:Legumins
D004268:DNA-Binding Proteins; D010940:Plant Proteins; D000097793:Legumins