Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase.
A highly cross-reactive monoclonal antibody (MAb), 2G10, was found to react in a conserved region of Escherichia coli RNA polymerase sigma70. The epitope was localized to amino acids 470 to 486, which included part of conserved region 3.1. The epitope for MAb 3D3, a MAb which maps close to the 2G10 epitope, was also determined.
Breyer
M J
MJ
McArdle Laboratory for Cancer Research, University of Wisconsin--Madison, 53706, USA.
Thompson
N E
NE
Burgess
R R
RR
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001419:Bacteria / Q000201:enzymology / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000276:immunology; D018604:Epitope Mapping; D000939:Epitopes / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology* / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D012808:Sigma Factor / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases