title: Transcription regulation of the PDGF A-chain gene by first intron elements.
abstract: A cis-acting regulatory region within the first intron of the human platelet-derived growth factor (PDGF) A-chain gene has been identified that functions to negatively regulate transcription of PDGF A-chain promoter/CAT reporter constructs in both A172 and HeLa cells and that functions independent of position, orientation, and promoter context. Further dissection of this region revealed several independently acting negative regulatory elements that exhibited cell-type specificity. These results suggest that the first intron of the PDGF A-chain gene contains negative regulatory elements that may cooperate to regulate the cell-type specific expression of the PDGF A-chain gene.
authors.affiliation: Department of Medicine, The Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA.
authors.forename: M
authors.initials: M
authors.lastname: Nobuyoshi
authors.forename: X H
authors.initials: XH
authors.lastname: Lin
authors.forename: Y
authors.initials: Y
authors.lastname: Takimoto
authors.forename: T F
authors.initials: TF
authors.lastname: Deuel
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007438:Introns / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D010982:Platelet-Derived Growth Factor; D013937:Thymidine Kinase