Tissue-specific subunit of the mouse cytosolic chaperonin-containing TCP-1.
We have cloned a novel Tcp-1-related mouse testis cDNA encoding a polypeptide of 531 amino acids which shares 81.2% identity with the zeta subunit of the mouse cytosolic chaperonin-containing TCP-1 (CCT). Immunoblot analysis of mouse testis CCT subunits separated by 2-dimensional gel electrophoresis indicates that this novel gene, Cctz-2, encodes a CCT subunit of Mr 57 000 and pI 7.1. Cctz-2 mRNA is detected only in testis whereas the other Cctz gene, Cctz-1, is expressed in all tissues investigated. The CCTzeta-2 subunit may have specific functions in the folding of testicular proteins and for interactions with testicular molecular chaperones.
Kubota
H
H
Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Hynes
G M
GM
Kerr
S M
SM
Willison
K R
KR
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D007526:Isoelectric Point; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D013737:Testis / Q000378:metabolism*
C533303:CCT6B protein, human; D018076:DNA, Complementary; C533315:Tcp1 protein, mouse; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins