title: High-performance liquid chromatography assay of N,N-dimethyl-p-toluidine released from bone cements: evidence for toxicity.
abstract: Five commercially available bone cements were analysed by high-performance liquid chromatography for detecting the residual content of an accelerator, the amine N,N-dimethyl-p-toluidine (DMPT), after curing. It was found that the concentration of DMPT in aqueous extracts decreases with time, being almost absent 7 days after curing. Differences were noticed among the cements; residual DMPT is higher in cements prepared with higher content of the amine. It is verified that DMPT's toxic effect on cell cultures is dose-related; a delay in the cell replication cycle is induced in vitro. Damage is reversible, thus justifying the low bone cement toxicity that is clinically ascertained.
authors.affiliation: Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Stea
authors.forename: D
authors.initials: D
authors.lastname: Granchi
authors.forename: C
authors.initials: C
authors.lastname: Zolezzi
authors.forename: G
authors.initials: G
authors.lastname: Ciapetti
authors.forename: M
authors.initials: M
authors.lastname: Visentin
authors.forename: D
authors.initials: D
authors.lastname: Cavedagna
authors.forename: A
authors.initials: A
authors.lastname: Pizzoferrato
mesh_terms: D001843:Bone Cements / Q000737:chemistry* / Q000633:toxicity; D001859:Bone Neoplasms; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007700:Kinetics; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D012516:Osteosarcoma; D014052:Toluidines / Q000032:analysis* / Q000633:toxicity; D014407:Tumor Cells, Cultured
chemical_list: D001843:Bone Cements; D014052:Toluidines; C015835:dimethyl-4-toluidine