Synthesis and utilization of GDP-D-arabinopyranoside.
We describe a procedure for the enzymatic synthesis of labeled or unlabeled GDP-D-arabinopyranoside. This method uses two enzymes purified from pig kidney: an L-fucokinase and a GDP-L-fucose pyrophosphorylase. The isolated GDP-D-[3H]arabinose served as a precursor for arabinose addition to lipophosphoglycan (LPG) of Leishmania major, using a parasite membrane fraction as the source of arabinosyltransferase. The procedures described provide a useful means for obtaining radiolabeled GDP-D-arabinopyranoside to study synthesis of D-arabinopyranoside-containing glycoconjugates.
Park
S
S
Department of Biochemistry and Molecular Biology, University of Arkanasas for Medical Sciences, Little Rock 72211, USA.
Pastuszak
I
I
Mengeling
B J
BJ
Turco
S J
SJ
Elbein
A D
AD
D000818:Animals; D001089:Arabinose / Q000031:analogs & derivatives*; D002850:Chromatography, Gel; D003421:Crithidia; D006156:Guanosine Diphosphate Sugars / Q000138:chemical synthesis* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D018320:Leishmania major; D009713:Nucleotidyltransferases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D013403:Sugar Phosphates / Q000138:chemical synthesis; D013552:Swine
D006156:Guanosine Diphosphate Sugars; D013403:Sugar Phosphates; C104973:arabinose-1-phosphate; C088421:guanidine diphosphate-arabinopyranose; D001089:Arabinose; D017853:Phosphotransferases (Alcohol Group Acceptor); C020153:fucokinase; D009713:Nucleotidyltransferases; C026916:fucose-1-phosphate guanylyltransferase