Clustering of Argentinean tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene.
The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were made. A partial sequence of the N gene was able to classify our local isolates within three Tospovirus species previously described (Tomato spotted wilt virus, TSWV; tomato chlorotic spot virus, TCSV and groundnut ringspot virus, GRSV). With the sequence data currently available, we performed a cladistic phylogenetic analysis which gave a possible genealogy among members of the Tospovirus genus.
Dewey
R
R
Instituto de Bioquímica y Biología Molecular (IBBM), Fac. Cs. Exactas, Universidad Nacional de La Plata, Argentina.
Semorille
L
L
Crisci
J
J
Grau
O
O
D000595:Amino Acid Sequence; D001118:Argentina; D001483:Base Sequence; D003001:Cloning, Molecular; D005814:Genes, Viral; D008969:Molecular Sequence Data; D019251:Nucleocapsid / Q000235:genetics*; D019590:Nucleocapsid Proteins; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid; D017865:Tospovirus / Q000145:classification* / Q000235:genetics; D014158:Transcription, Genetic
D019590:Nucleocapsid Proteins; D012367:RNA, Viral; C099603:nucleocapsid protein, Tospovirus