Detection of mutagenicity in Ames test using a metalloporphyrin/oxidant model system for cytochrome P450.
A chemical model system for cytochrome P450, a porphyrin and an oxidant, was used in Ames assay as a substitute for S9 mix. In the presence of tetrakis(pentafluorophenyl)porphyrinatoiron(III) chloride [Fe(F5P)Cl] and tert-butyl hydroperoxide (t-BuOOH), mutagenicity of N-nitrosodibutylamine (NDB) in Salmonella typhimurium TA1535 was detected. The mutagenicity depended on the pre-incubation period, and also on the concentration of an oxidant and of bacteria. In the chemical model system, pH affected the mutagenicity of NDB, which suggested that as observed in an enzymatic activating system, the mutagenicity was due to the labile alkylating species which was derived from NDB activated in the chemical activation system and was sensitive to pH. Under the optimum conditions; a higher concentration of an oxidant, a higher concentration of bacterial culture, and a weakly acidic medium, mutagenicity of N-nitrosodipropylamine in S. typhimurium TA1535 was also detected. Besides N-nitrosodialkylamines, 2-aminofluorene (2-AF) and benzo[a]pyrene (BaP) were also used as mutagens. Mutagenicity of 2-AF and BaP in S. typhimurium TA1538 were both detected in the same system as used in detecting the mutagenicity of N-nitrosodialkylamines. Ames test using a metalloporphyrin/oxidant model system makes it possible to detect mutagenicity derived from both base pair substitution mutagens and frameshift mutagens without using enzymatic activating system. These results demonstrate that the assay with the chemical model system is useful in detecting unstable unknown active mutagens or investigating the mechanisms of the metabolic pathway of mutagens or carcinogens in a protein-free medium.
Okochi
E
E
Kyoritsu College of Pharmacy, Tokyo, Japan.
Kurahashi
A
A
Mochizuki
M
M
D001564:Benzo(a)pyrene / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005290:Ferric Compounds / Q000494:pharmacology; D005449:Fluorenes / Q000494:pharmacology; D016368:Frameshift Mutation; D006863:Hydrogen-Ion Concentration; D008665:Metalloporphyrins / Q000494:pharmacology; D009152:Mutagenicity Tests; D009602:Nitrosamines / Q000494:pharmacology*; D016877:Oxidants / Q000378:metabolism; D010545:Peroxides / Q000494:pharmacology; D017354:Point Mutation; D012486:Salmonella typhimurium / Q000235:genetics*; D020122:tert-Butylhydroperoxide; D005464:Fluorobenzenes; D005466:Fluorocarbons
D001564:Benzo(a)pyrene; D002273:Carcinogens; D003577:Cytochrome P-450 Enzyme System; D005290:Ferric Compounds; D005449:Fluorenes; D008665:Metalloporphyrins; D009602:Nitrosamines; D016877:Oxidants; D010545:Peroxides; D020122:tert-Butylhydroperoxide; C093411:tetrakis(pentafluorophenyl)porphyrinato iron(III) chloride; D005464:Fluorobenzenes; D005466:Fluorocarbons; C013161:N-nitroso(di-n-propyl)amine; C012177:2-aminofluorene; C012279:dibutylnitrosamine