The tyrosine kinase inhibitor methyl 2,5-dihydroxycinnimate disrupts changes in the actin cytoskeleton required for neurite formation.
In the current studies, we investigated the relationship between tyrosine phosphorylation and neurite formation. In SH-SY5Y neuroblastoma cells, the tyrosine kinase inhibitor methyl 2, 5-dihydroxycinnimate blocked neurite formation on laminin. This corresponded with inhibition of paxillin and focal adhesion kinase tyrosine phosphorylation as well as a disruption of actin filament organization and actin polymerization. This suggests that tyrosine phosphorylation helps direct changes in the actin cytoskeleton required for neurite formation.
Leventhal
P S
PS
Department of Neurology, University of Michigan, Ann Arbor 48109-0588, USA.
Feldman
E L
EL
D000199:Actins / Q000378:metabolism*; D002478:Cells, Cultured; D002934:Cinnamates / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D016501:Neurites / Q000187:drug effects*
D000199:Actins; D002934:Cinnamates; C067472:methyl 2,5-dihydroxycinnamate