title: The MIC1 microneme protein of Toxoplasma gondii contains a duplicated receptor-like domain and binds to host cell surface.
abstract: The cDNA encoding the Toxoplasma gondii microneme protein MIC1 and the corresponding gene have been cloned and sequenced. The MIC1 gene contains three introns. The cDNA encodes a 456 amino acid (aa) sequence, with a typical signal sequence and no other trans-membrane domain. The protein contains a tandemly duplicated domain with conservation of cysteines and presents distant homology with the Plasmodium sp. microneme protein TRAP-SSP2. The MIC1 protein from tachyzoite lysates and a PMAL recombinant expressing the N-terminal duplicated domain of the protein bound to the surface of putative host cells, suggesting a possible involvement of MIC1 in host cell binding/recognition.
authors.affiliation: INSERM U42, Villeneuve d' Ascq, France.
authors.forename: M N
authors.initials: MN
authors.lastname: Fourmaux
authors.forename: A
authors.initials: A
authors.lastname: Achbarou
authors.forename: O
authors.initials: O
authors.lastname: Mercereau-Puijalon
authors.forename: C
authors.initials: C
authors.lastname: Biderre
authors.forename: I
authors.initials: I
authors.lastname: Briche
authors.forename: A
authors.initials: A
authors.lastname: Loyens
authors.forename: C
authors.initials: C
authors.lastname: Odberg-Ferragut
authors.forename: D
authors.initials: D
authors.lastname: Camus
authors.forename: J F
authors.initials: JF
authors.lastname: Dubremetz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014122:Toxoplasma / Q000235:genetics* / Q000378:metabolism; D014709:Vero Cells
chemical_list: D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; C105877:MIC1 protein, Toxoplasma gondii; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins