title: In vitro activity of omeprazole in combination with several antimicrobial agents against clinical isolates of Helicobacter pylori.
abstract: An agar dilution checkerboard method was used to evaluate the in vitro activity of omeprazole combined with clarithromycin, amoxicillin, and ceftibuten, respectively, against clinical isolates of Helicobacter pylori. Mueller-Hinton agar plus 5% horse blood, an inoculum of 10(6) cfu/ml, and incubation of 72 h in a CO2 atmosphere were used. With the omeprazole and clarithromycin combination, synergism was observed in 51.5% and partial synergism in 39.3% of 33 strains; with omeprazole and amoxicillin, synergism was observed in 3% and partial synergism in 60.6% of 33 strains; and with omeprazole and ceftibuten, synergism was observed in 33.3% and partial synergism in 50% of 24 strains. Antagonism between omeprazole and each antibiotic was exhibited in 0%, 6.1%, and 4.1% of these groups of strains, respectively. Of the antibiotic combinations tested, omeprazole plus clarithromycin exhibited synergism (partial or total) in the highest percentage of strains.
authors.affiliation: Department of Microbiology, Hospital Universitario de la Princesa, Diego de León, Madrid, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Alarcón
authors.forename: D
authors.initials: D
authors.lastname: Domingo
authors.forename: I
authors.initials: I
authors.lastname: Sánchez
authors.forename: F
authors.initials: F
authors.lastname: Díaz de Rojas
authors.forename: M
authors.initials: M
authors.lastname: López-Brea
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D001706:Biopsy; D000077722:Ceftibuten; D002511:Cephalosporins / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology*; D004336:Drug Antagonism; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D004724:Endoscopy; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009853:Omeprazole / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology*; D013270:Stomach / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D000897:Anti-Ulcer Agents; D002511:Cephalosporins; D010406:Penicillins; D000658:Amoxicillin; D017291:Clarithromycin; D000077722:Ceftibuten; D009853:Omeprazole