title: Transient up-regulation of P2Y2 nucleotide receptor mRNA expression is an immediate early gene response in activated thymocytes.
abstract: In studies designed to understand the roles of P2 nucleotide receptors in differentiation of T lymphocytes, we observed a transient and protein synthesis-independent enhancement of mRNA expression for the G protein-coupled P2Y2 receptor in mouse thymocytes after the addition of steroid hormone or T cell receptor (TCR) crosslinking by anti-TCR mAb. Conversely, dexamethasone-induced increases in mRNA expression for the ligand-gated ion channel P2X1 receptor was detected in rat, but not mouse, thymocytes, raising questions about the previously suggested role of P2X1 receptors in thymocyte apoptosis. Flow cytometry analysis of thymocyte subsets excluded the possibility that the observed increases in P2Y2 receptor mRNA expression were due to the enrichment of steroid-treated cells with an P2Y2 mRNA-rich thymocyte subset. Triggering of TCR-mediated intracellular signaling pathways through crosslinking of TCR or by addition of phorbol ester and Ca2+ ionophore also resulted in the up-regulation of P2Y2, but not P2X1, receptor mRNA. It is proposed that the rapid increase of P2Y2 receptor mRNA expression could be a common early event in responses of T cells to different activating stimuli. Taken together with the recently discovered ability of nucleotide receptor-initiated signaling to antagonize or enhance the effects of TCR crosslinking or steroids on thymocytes, the observed rapid up-regulation of P2Y2 receptor mRNA expression may reflect an immediate early gene response where newly expressed cell surface nucleotide receptors provide regulatory feedback signaling from extracellular ATP in the T cell differentiation process.
authors.affiliation: Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: M
authors.initials: M
authors.lastname: Koshiba
authors.forename: S
authors.initials: S
authors.lastname: Apasov
authors.forename: V
authors.initials: V
authors.lastname: Sverdlov
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Erb
authors.forename: J T
authors.initials: JT
authors.lastname: Turner
authors.forename: G A
authors.initials: GA
authors.lastname: Weisman
authors.forename: M V
authors.initials: MV
authors.lastname: Sitkovsky
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D017781:Genes, Immediate-Early / Q000276:immunology*; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics*; D016131:Lymphocyte Subsets; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D018048:Receptors, Purinergic P2 / Q000235:genetics*; D058469:Receptors, Purinergic P2X; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
chemical_list: D005938:Glucocorticoids; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D003907:Dexamethasone; D003513:Cycloheximide