Effects of a nitric oxide donor and nitric oxide synthase inhibitors on acid secretion of isolated rabbit gastric glands.
The present study aims to investigate the effect of nitric oxide (NO) on histamine-stimulated acid secretion of gastric glands. An NO donor, sodium nitroprusside (SNP), and inhibitors of NO synthase such as NG-nitro-L-arginine methyl ester (L-NAME) and NG-nitro-L-arginine (L-NNA) with or without L-arginine, a substrate for NO synthase, were added to isolated rabbit gastric glands with the secretagogues. Acid secretion, intracellular concentrations of cGMP and cAMP and lactic dehydrogenase (LDH) release were determined. L-NAME and L-NNA increased stimulated acid secretion, which was reversed by L-arginine. SNP inhibited stimulated acid secretion concentration-dependently; the inhibition was accompanied by an increase in the intracellular cGMP, but neither related to cAMP nor LDH release. In conclusion, NO produced in large quantity by an NO donor inhibits histamine-stimulated acid secretion of isolated rabbit gastric glands.
Kim
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Department of Pharmacology, Yonsei University College of Medicine, Seoul, Korea.
Kim
K H
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D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D019335:Nitroarginine; D009599:Nitroprusside / Q000494:pharmacology*; D011817:Rabbits
D009599:Nitroprusside; D019335:Nitroarginine; D009569:Nitric Oxide; D006632:Histamine; D000242:Cyclic AMP; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP; D019331:NG-Nitroarginine Methyl Ester