Intravenous catecholamines alter hepatic blood flow in conscious dogs with experimental hepatic denervation.
Hepatic blood flow is regulated by the autonomic nervous system; however, blood flow through the denervated liver has been controversial. The purpose of this study is to evaluate the changes in hepatic blood flow caused by experimental hepatic denervation during the intravenous infusions of catecholamines (dopamine or dobutamine) with or without prior administration of alpha-adrenoceptor antagonist (phenoxybenzamine) or beta-adrenoceptor antagonist (propranolol). The liver blood flow was measured using transit time ultrasonic flow meter probes at the portal vein and hepatic artery in conscious dogs which (a) underwent hepatic denervation (denervation group, n = 9) and (b) were intact (control group, n = 10). Norepinephrine concentrations in the liver were determined to evaluate the effects of hepatic denervation and were decreased at 1 week and 4 weeks after hepatic denervation. In the control group, dopamine and dobutamine produced an increase of portal venous blood flow (PVF). Conversely, hepatic denervation reduced the increase in PVF by dopamine and dobutamine. Dopamine with prior administration of phenoxybenzamine produced a much larger increase in PVF in both groups. Pretreatment of propranolol in both groups abolished the increasing effects of dopamine and dobutamine in PVF. Dopamine reduced the hepatic arterial blood flow (HAF) regardless of hepatic denervation. During dobutamine infusion, HAF was decreased by hepatic denervation and prior administration of propranolol. These results suggest that hepatic denervation reduces the increasing effects of catecholamines on the hepatic blood flow through greater enhancement of alpha-adrenergic effects than of beta-adrenergic effects in the hepatic vascular autonomic nerve response.
Kato
M
M
First Department of Surgery, Nagoya University School of Medicine, Japan.
Nimura
Y
Y
Miyachi
M
M
Kitagawa
Y
Y
Watanabe
T
T
Kawabata
Y
Y
Akiyama
H
H
D000818:Animals; D002395:Catecholamines / Q000097:blood*; D004280:Dobutamine / Q000494:pharmacology; D004285:Dogs; D004298:Dopamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D008099:Liver / Q000098:blood supply* / Q000294:innervation*; D008102:Liver Circulation / Q000187:drug effects*; D008297:Male; D012039:Regional Blood Flow / Q000187:drug effects
D002395:Catecholamines; D004280:Dobutamine; D004298:Dopamine