The TEL gene products: nuclear phosphoproteins with DNA binding properties.
The human TEL gene is involved in several 12p13 chromosomal abnormalities present in various human hematological malignancies, the most frequent being the t(12;21)(p13;q22), specific for childhood acute lymphoblastic leukemia. The predicted product of TEL harbours an amino acid region similar to the ETS DNA binding domain. We now report the isolation of the murine TEL cDNA and the characterization of the human TEL proteins. Human and murine TEL proteins are particularly homologous within their aminoterminal regions and their ETS domains. TEL proteins are nuclear and display specific DNA binding activity toward classical ETS binding sites. In addition, we show that TEL mRNAs initiate translation at either of the two first inframe ATGs (codon 1 and 43) to encode 50 kDa and 57 kDa TEL proteins. In vivo, each of these primary translational products is modified by multiple phosphorylation events.
Poirel
H
H
U 301 de l'Institut National de la Santé et de la Recherche Médicale (INSERM) and SD 401 No. 301 CNRS, Paris, France.
Oury
C
C
Carron
C
C
Duprez
E
E
Laabi
Y
Y
Tsapis
A
A
Romana
S P
SP
Mauchauffe
M
M
Le Coniat
M
M
Berger
R
R
Ghysdael
J
J
Bernard
O A
OA
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D019556:COS Cells; D002881:Chromosomes, Human, Pair 12; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015448:Leukemia, B-Cell / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000302:isolation & purification; D010750:Phosphoproteins / Q000235:genetics* / Q000302:isolation & purification; D010766:Phosphorylation; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger / Q000302:isolation & purification; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured; D000097764:ETS Translocation Variant 6 Protein
D004247:DNA; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D000097764:ETS Translocation Variant 6 Protein