QM is a novel zinc-binding transcription regulatory protein: its binding to c-Jun is regulated by zinc ions and phosphorylation by protein kinase C.
A novel method was developed for cloning of zinc-binding proteins. We used 65Zn2+ as a probe to screen a human lung cDNA library, and isolated QM using this approach. QM appears to be a negative regulator of c-Jun that acts by binding to the leucine zipper region of c-Jun. We demonstrated that QM bound zinc ions and that such binding was necessary for the interaction of QM with c-Jun. We also showed that protein kinase C introduced about 1 mol of phosphate into 1 mol of QM. The binding of QM to c-Jun was decreased by 60% when QM had been phosphorylated. These results suggest that QM is a novel zinc-binding transcription regulatory protein and that interaction between QM and c-Jun is regulated by zinc ions and phosphorylation.
Inada
H
H
Department of Molecular and Cellular Pharmacology, Mie University School of Medicine, Tsu, Japan.
Mukai
J
J
Matsushima
S
S
Tanaka
T
T
D001665:Binding Sites; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D015723:Gene Library; D005982:Glutathione Transferase; D006801:Humans; D007700:Kinetics; D016350:Leucine Zippers; D008168:Lung / Q000378:metabolism; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins; D014157:Transcription Factors / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D025521:Tumor Suppressor Proteins; D015032:Zinc / Q000378:metabolism* / Q000494:pharmacology
D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D016755:Proto-Oncogene Proteins c-jun; C000630686:RPL10 protein, human; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D005982:Glutathione Transferase; D011493:Protein Kinase C; D015032:Zinc