title: False positives from the yeast two-hybrid system.
abstract: Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methods-reagents, available on the Internet. This month's column discusses false positives found when using the yeast two-hybrid system to isolate interactive proteins. For details on how to partake in the newsgroup, see the accompanying box.
authors.affiliation: National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA. pnh@ncifcrf.gov
authors.forename: P N
authors.initials: PN
authors.lastname: Hengen
mesh_terms: D005978:Glutathione / Q000096:biosynthesis / Q000235:genetics; D006824:Hybridization, Genetic / Q000235:genetics*; D011237:Predictive Value of Tests; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D011993:Recombinant Fusion Proteins; D005978:Glutathione