Reduced glutathione and S-acetylglutathione as selective apoptosis-inducing agents in cancer therapy.
The effect of reduced glutathione (GSH) and S-acetylglutathione (S-acglu) treatment on several tumor cell lines and normal cells in vitro was investigated. GSH and S-acglu applied at concentrations of 1 mM and 2 mM induced apoptosis in malignant cells as shown by DNA-fragmentation and staining of apoptotic cells with 7-amino-actinomycin D while viability and growth of normal cells were not significantly influenced by this treatment. The results demonstrated that GSH and S-acglu may be selective inducers of apoptosis in malignant cells.
Donnerstag
B
B
Department of Biological Chemistry, University Medical Center, Franfurt am Main, Germany.
Ohlenschlager
G
G
Cinatl
J
J
Amrani
M
M
Hofmann
D
D
Flindt
S
S
Treusch
G
G
Träger
L
L
D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D004587:Electrophoresis, Agar Gel; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006801:Humans; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D004273:DNA, Neoplasm; C096306:S-acetylglutathione; D005978:Glutathione