title: [Role of intercellular adhesion molecule (ICAM)-1 in the interaction between glomerular endothelial cells and granulocytes].
abstract: The present study was designed to investigate the role of intercellular adhesion molecule (ICAM)-1 in the interaction between glomerular endothelial cells (GEN) and granulocytes. Expression of ICAM-1 was promoted after stimulation with tumor necrosis factor (TNF)-alpha. This effect was dose- and time-dependent. Granulocytes attached to GEN in accordance with the increased expression of ICAM-1 on the cells stimulated with TNF-alpha. In addition, the adhesion of granulocytes to activated GEN with TNF-alpha stimulated specific [51Cr] release from the monolayers. Furthermore, monoclonal antibodies to TNF-alpha dose-dependently inhibited the adhesion of granulocytes to activated GEN. In summary, ICAM-1 is related to the glomerular endothelial cell injury induced by granulocytes activated with TNF-alpha, suggesting that ICAM-1 may play a role in the initiation of certain types of glomerulonephritis with granulocyte infiltration.
authors.affiliation: Department of Medicine, Tokyo Women's Medical College, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Miwa
authors.forename: K
authors.initials: K
authors.lastname: Nitta
authors.forename: W
authors.initials: W
authors.lastname: Yumura
authors.forename: H
authors.initials: H
authors.lastname: Nihei
mesh_terms: D000818:Animals; D002417:Cattle; D002450:Cell Communication / Q000502:physiology*; D004727:Endothelium / Q000166:cytology; D006098:Granulocytes / Q000166:cytology*; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D007678:Kidney Glomerulus / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1