Direct detection of Campylobacter jejuni in chicken cecal contents by PCR.
Campylobacter jejuni in chicken feces was detected by PCR and Southern blot hybridization (SBH). The detection limits of C. jejuni in chicken feces were 34,000 cells by PCR and 340 cells by SBH. Some cecal contents of chickens up to 3 weeks old were C. jejuni positive by SBH whereas all of them were negative by PCR. Two of 51 cecal contents of 18-day-old chicken embryos were C. jejuni positive by PCR and SBH; but, C. jejuni were not isolated from the samples by conventional culture with selective enrichment.
Chuma
T
T
Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University, Japan.
Yano
K
K
Omori
H
H
Okamoto
K
K
Yugi
H
H
D000375:Aging; D000818:Animals; D015139:Blotting, Southern / Q000379:methods; D016123:Campylobacter jejuni / Q000302:isolation & purification*; D002432:Cecum / Q000382:microbiology*; D002642:Chick Embryo; D002645:Chickens / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005766:Gastrointestinal Contents / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity