Evidence that heteronuclear proteins interact with XIST RNA in vitro.
The process of X chromosome inactivation results in the transcriptional silencing of one of the two X chromosomes in mammalian females. A large heterogeneous nuclear RNA that is expressed exclusively from the inactive X chromosome (XIST--X Inactive Specific Transcripts) has been implicated in the inactivation process. The XIST RNA colocalizes with the inactive X chromosome and therefore proteins that interact with the XIST RNA may be involved in the inactivation of the X chromosome. In order to identify such proteins we have used an in vitro UV light cross-linking technique to detect nuclear proteins associating with sections of the XIST RNA. The strongest interaction detected by this technique was between a pair of approximately 40 kDa proteins and a 5' region of the XIST RNA which contains a series of well-conserved tandem repeats. Immunoprecipitation suggested that these proteins may be the heteronuclear proteins hnRNPC1/C2.
Brown
C J
CJ
Department of Medical Genetics, University of British Columbia, Vancouver.
Baldry
S E
SE
D000818:Animals; D001667:Binding, Competitive; D005786:Gene Expression Regulation; D006801:Humans; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D012332:RNA, Heterogeneous Nuclear / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic; D014960:X Chromosome
D012332:RNA, Heterogeneous Nuclear; D016601:RNA-Binding Proteins