title: Immune bioactivity in shellfish toward serum-free cultured human cell lines.
abstract: The biologically functional effect of eight kinds of hot-water extracts of shellfish on cultured human cell lines was examined in a serum-free medium model. Meretrix lusoria and Sinonovacula constricta extracts enhanced IgM secretion of both hybridoma HB4C5 and SI102 cells when cultured with the respective extracts. The purified principle exhibited remarked activity in the adsorbed fraction in hydroxyapatite and Concanavalin A columns. The extracts of Corbicula fluminea, Crassostreas gigas, Meretrix lusoria, Anadara granosa, and Sinonovacula constricta enhanced in nitroblue tetrazolium (NBT)-reducing ability of macrophage U-M cells. Meretrix lusoria, Anadara granosa, and Sinonovacula constricta were specifically cytotoxic to both cultures of MCF-7 breast cancer cells and HuH-6KK hepatoblastoma. These findings imply that the extracts of shellfish that were examined exhibited a differential effect on immune cells and tumor cells.
authors.affiliation: Department of Marine Food Science, National Taiwan Ocean University, Keelung, Republic of China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Kong
authors.forename: L C
authors.initials: LC
authors.lastname: Chiang
authors.forename: F
authors.initials: F
authors.lastname: Fang
authors.forename: K
authors.initials: K
authors.lastname: Shinohara
authors.forename: P
authors.initials: P
authors.lastname: Pan
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D005591:Chemical Fractionation; D016895:Culture Media, Serum-Free; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D006825:Hybridomas / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D007075:Immunoglobulin M / Q000187:drug effects / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D012758:Shellfish; D014020:Tissue Extracts / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology; D014867:Water
chemical_list: D016895:Culture Media, Serum-Free; D007075:Immunoglobulin M; D014020:Tissue Extracts; D014867:Water; D009580:Nitroblue Tetrazolium