Determination of 2-keto-3-deoxyoctulosonic acid (KDO) with high-performance anion-exchange chromatography (HPAEC): survey of stability of KDO and optimal hydrolytic conditions.
A new method for 2-keto-3-deoxy-octulosonic acid (KDO) determination using high-performance anion-exchange chromatography was developed. KDO is well separated from Neu5Ac, and the response was linear from 20 pmol to 5 nmol. The method was used to examine the stability of KDO under various hydrolytic, conditions and to survey optimal hydrolytic conditions for release of KDO.
Kiang
J
J
Biology Department, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Szu
S C
SC
Wang
L X
LX
Tang
M
M
Lee
Y C
YC
D002852:Chromatography, Ion Exchange / Q000379:methods*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000378:metabolism; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010504:Periodic Acid; D012475:Salmonella; D013400:Sugar Acids / Q000032:analysis*
D008070:Lipopolysaccharides; D013400:Sugar Acids; D010504:Periodic Acid; C002532:2-keto-3-deoxyoctonate; C009288:metaperiodate