Hamster cardiac xenograft in rat ear: effect of cyclosporine A and leflunomide on survival.
The survival was determined of hamster-heart grafts subcutaneously placed in rat ears. Thirteen out of the 14 grafts tested in rats without immunosuppression were rejected by day 7 post-transplantation, and the one graft was rejected by day 9. Cyclosporine A fully protected the grafts for 2 weeks, the entire experimental period. By contrast, leflunomide seemed only to delay the graft rejection. The formation of antidonor antibodies was fully suppressed by both drugs. These results reveal a pivotal role of T-cells in this model. The results with leflunomide suggest that this drug preferentially affected humoral responses in our study.
Ikeda
K
K
Infectious Disease and Immunology Research Laboratory, Yamanouchi Pharmaceutical Co. Ltd, Ibaraki Prefecture, Japan.
Hirano
M
M
Takeuchi
M
M
Yamada
T
T
Tomioka
K
K
D000818:Animals; D000831:Animals, Newborn; D006224:Cricetinae; D016572:Cyclosporine / Q000494:pharmacology*; D004423:Ear / Q000502:physiology*; D005260:Female; D006085:Graft Survival / Q000187:drug effects*; D016027:Heart Transplantation / Q000276:immunology / Q000502:physiology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007555:Isoxazoles / Q000494:pharmacology*; D000077339:Leflunomide; D008297:Male; D008647:Mesocricetus; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014183:Transplantation, Heterologous / Q000276:immunology / Q000502:physiology*
D007166:Immunosuppressive Agents; D007555:Isoxazoles; D016572:Cyclosporine; D000077339:Leflunomide