Identification of a human endogenous LTR-like sequence using HIV-1 LTR specific primers.
Using 'consensus' primers derived from the LTR region of 15 HIV-1 isolates, a fragment of 583 bp was amplified from human DNA. Even though specificity was confirmed by Southern blot analysis with a conserved LTR oligonucleotide probe, no significant homologies were detected to either retroviral regions or human or non-human published sequences. Nevertheless, when used as a probe, the 583-bp fragment identified a unique DNA sequence in the human genome on chromosome 1, and cross-reactive sequences in monkey, but not mouse, DNA. This novel, unique and conserved sequence of 583 bp was used to isolate a human HS-1 clone in which the structural property of a viral LTR could be identified.
Menin
C
C
Istituto di Oncologia, University of Padova, Italy.
Indraccolo
S
S
Montagna
M
M
Corneo
B
B
Bonaldi
L
L
Leib-Mösch
C
C
Chieco-Bianchi
L
L
D'Andrea
E
E
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D017931:DNA Primers; D004247:DNA