Cloning and characterization of the groE locus from Actinobacillus pleuropneumoniae.
A 4.4-kb DNA fragment was cloned from Actinobacillus pleuropneumoniae (strain 4074, serotype 1) by genetic complementation with Escherichia coli groES-groEL mutant strains. Sequence analysis of this fragment revealed a purine nucleoside phosphorylase (DeoD)-encoding gene homolog (deoD), heat-shock response-encoding genes for the small (groES) and large subunits (groEL) and a partial open reading frame encoding an alcohol dehydrogenase homolog (adhE). The predicted amino-acid sequence of groES and groEL genes showed extensive sequence identity (80-95%) with other Pasteurellaceae. The gene organization surrounding the groE locus was different from that of Haemophilus infuenzae. When expressed in E. coli, groES-groEL genes were capable of complementing the growth of a lambda lytic phage, indicating a structural as well as functional conservation.
Vézina
G
G
Département de Chimie-Biologie, Université du Québec à Trois-Rivières, Canada.
Sirois
M
M
Clairoux
N
N
Boissinot
M
M
D016977:Actinobacillus pleuropneumoniae / Q000201:enzymology / Q000235:genetics*; D000426:Alcohol Dehydrogenase / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D018833:Chaperonins; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011683:Purine-Nucleoside Phosphorylase / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C028715:GroE protein, E coli; D006360:Heat-Shock Proteins; D000426:Alcohol Dehydrogenase; D011683:Purine-Nucleoside Phosphorylase; D018833:Chaperonins