Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5.
A complementary DNA clone has been isolated that encodes a coxsackievirus and adenovirus receptor (CAR). When transfected with CAR complementary DNA, nonpermissive hamster cells became susceptible to coxsackie B virus attachment and infection. Furthermore, consistent with previous studies demonstrating that adenovirus infection depends on attachment of a viral fiber to the target cell, CAR-transfected hamster cells bound adenovirus in a fiber-dependent fashion and showed a 100-fold increase in susceptibility to virus-mediated gene transfer. Identification of CAR as a receptor for these two unrelated and structurally distinct viral pathogens is important for understanding viral pathogenesis and has implications for therapeutic gene delivery with adenovirus vectors.
Bergelson
J M
JM
Division of Infectious Diseases, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
Cunningham
J A
JA
Droguett
G
G
Kurt-Jones
E A
EA
Krithivas
A
A
Hong
J S
JS
Horwitz
M S
MS
Crowell
R L
RL
Finberg
R W
RW
D000260:Adenoviruses, Human / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D029822:Enterovirus B, Human / Q000378:metabolism* / Q000502:physiology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment; D014162:Transfection; D014779:Virus Replication
C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus