title: Leishmania aethiopica derived from diffuse leishmaniasis patients preferentially induce mRNA for interleukin-10 while those from localized leishmaniasis patients induce interferon-gamma.
abstract: Exposure of peripheral blood mononuclear cells to promastigotes of Leishmania aethiopica derived from patients with the self-limiting, localized form of the disease (local cutaneous leishmaniasis; LCL) preferentially induced mRNA for interferon (IFN)-gamma but little for interleukin (IL)-10. In contrast, stimulation of the same cells with promastigotes derived from patients with the persistent, disseminated form of the disease (diffuse cutaneous leishmaniasis; DCL) stimulated the expression of IL-10 rather than IFN-gamma. In general, parasites derived from LCL patients induced more expression of other cytokines tested, including IL-4, IL-6, and transforming growth factor-beta, although tumor necrosis factor-alpha was equivalent in cultures stimulated with LCL or DCL promastigotes. The results suggest that the antigen-specific immunosuppression observed in DCL patients and the resulting clinical picture could in part be due to the properties of the infecting parasite to induce more IL-10 than IFN-gamma.
authors.affiliation: Section of Infectious Disease, Karolinska Institute at Huddinge Hospital, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Akuffo
authors.forename: K
authors.initials: K
authors.lastname: Maasho
authors.forename: M
authors.initials: M
authors.lastname: Blostedt
authors.forename: B
authors.initials: B
authors.lastname: Höjeberg
authors.forename: S
authors.initials: S
authors.lastname: Britton
authors.forename: M
authors.initials: M
authors.lastname: Bakhiet
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D007891:Leishmania / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D016753:Interleukin-10; D007371:Interferon-gamma