Analysis of whole-cell fatty acid profiles of verotoxigenic Escherichia coli and Salmonella enteritidis with the microbial identification system.
Differentiation of strains within bacterial species, based on gas chromatographic analysis of whole-cell fatty acid profiles, was assessed with 115 strains of verotoxigenic Escherichia coli and 315 strains of Salmonella enteritidis. Fatty acid-based subgroups within each of the two species were generated. Variability of fatty acid profiles observed in repeat preparations from the same strain approached that observed between subgroups, limiting the usefulness of using fatty acid profiles to subgroup verotoxigenic E. coli and S. enteritidis strains.
Steele
M
M
Agriculture and Food Laboratory Services Centre, Ontario Ministry of Agriculture Food and Rural Affairs, Guelph, Canada.
McNab
W B
WB
Read
S
S
Poppe
C
C
Harris
L
L
Lammerding
A M
AM
Odumeru
J A
JA
D001427:Bacterial Toxins / Q000096:biosynthesis*; D015373:Bacterial Typing Techniques; D002849:Chromatography, Gas; D004926:Escherichia coli / Q000737:chemistry / Q000145:classification*; D005227:Fatty Acids / Q000032:analysis*; D015203:Reproducibility of Results; D012477:Salmonella enteritidis / Q000737:chemistry / Q000145:classification*; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D005227:Fatty Acids; D022622:Shiga Toxin 1