Topical liposome delivery of molecules to hair follicles in mice.
The hair cycle consisting of growing and resting phases, is subject to widespread disease such as androgenic alopecia or loss of pigment which are in need of effective, targeted therapeutics. In order to develop a hair-follicle delivery system we demonstrate here that phosphatidylcholine liposomes entrapping either the fluorescent dye calcein or the pigment melanin can deliver these molecules into the hair follicle and hair shafts of mice when applied topically. Liposomal delivery of these molecules is time dependent. Negligible amounts of delivered molecules enter the dermis, epidermis or blood stream thereby demonstrating the enrichment of follicle delivery. Naked calcein and melanin are trapped in the stratum corneum and are unable to enter the follicle. The potential of the hair-follicle liposome delivery system for therapeutic use for hair disease is discussed.
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AntiCancer Inc., San Diego, CA 92111, USA.
Hoffman
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RM
D000287:Administration, Topical; D000818:Animals; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005069:Evaluation Studies as Topic; D005452:Fluoresceins / Q000008:administration & dosage / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000493:pharmacokinetics; D006201:Hair Diseases / Q000188:drug therapy; D018859:Hair Follicle / Q000187:drug effects* / Q000378:metabolism; D008081:Liposomes; D008543:Melanins / Q000008:administration & dosage / Q000493:pharmacokinetics; D051379:Mice; D013050:Spectrometry, Fluorescence
D004337:Drug Carriers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; D008543:Melanins; C007740:fluorexon