title: Incidence of apoptotic bodies in membrana granulosa of the patients participating in an in vitro fertilization program.
abstract: OBJECTIVE
  To investigate the incidence of apoptotic bodies in mural granulosa cell masses and cumulus cell masses.
  
  
  METHODS
  Nonrandomized, prospective study.
  
  
  METHODS
  Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, Japan.
  
  
  METHODS
  One hundred twenty-nine normally ovulating women underwent ovulation induction for IVF-ET with GnRH analogue (GnRH-a) and gonadotropins.
  
  
  METHODS
  Patients underwent follicle aspiration after the administration of hCG.
  
  
  METHODS
  The nuclei of recovered granulosa cells were examined by fluorescence microscopy and the incidence of apoptotic bodies was tabulated.
  
  
  RESULTS
  The incidence of apoptotic bodies was significantly higher in mural granulosa cell masses than in cumulus cell masses in the entire group of 129 patients. Both incidence of apoptotic bodies of mural granulosa cell masses and cumulus cell masses were significantly higher in patients with less than six follicular oocytes compared with patients with six or more oocytes. Nonpregnant patients showed significantly higher incidence of apoptotic bodies in mural granulosa cell masses compared with pregnant patients.
  
  
  CONCLUSIONS
  These results indicate that mural granulosa cell masses and cumulus cell masses may have different functions in follicular maturation. The incidence of apoptotic bodies in mural granulosa cell masses can be used as an indicator of success of IVF.
authors.affiliation: Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakahara
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: N
authors.initials: N
authors.lastname: Ohta
authors.forename: N
authors.initials: N
authors.lastname: Sakai
authors.forename: N
authors.initials: N
authors.lastname: Tezuka
authors.forename: M
authors.initials: M
authors.lastname: Hiroi
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000328:Adult; D017209:Apoptosis; D002452:Cell Count; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D007247:Infertility, Female / Q000473:pathology; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology; D006080:Ovarian Follicle / Q000473:pathology*; D011247:Pregnancy; D011446:Prospective Studies