title: Strongylus asini (Nematoda, Strongyloidea): genetic relationships with other Strongylus species determined by ribosomal DNA.
abstract: Genomic DNA was isolated from adult Strongylus asini collected from zebra. The second ribosomal transcribed spacer (ITS-2) was amplified and sequenced using polymerase chain reaction (PCR) based techniques. The DNA sequence was compared with previously published data for 3 related Strongylus species. A PCR-linked restriction fragment length polymorphism method allowed the 4 species to be differentiated unequivocally. The ITS-2 sequence of S. asini was found to be more similar to those of S. edentatus (87.1%) and S. equinus (95.3%) than to that of S vulgaris (73.9%). This result confirms that S. Asini and S vulgaris represent separate species and supports the retention of the 4 species within 1 genus.
authors.affiliation: Department of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia.
authors.forename: G C
authors.initials: GC
authors.lastname: Hung
authors.forename: D E
authors.initials: DE
authors.lastname: Jacobs
authors.forename: R C
authors.initials: RC
authors.lastname: Krecek
authors.forename: R B
authors.initials: RB
authors.lastname: Gasser
authors.forename: N B
authors.initials: NB
authors.lastname: Chilton
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009276:Namibia; D010527:Perissodactyla / Q000469:parasitology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013019:South Africa; D013323:Strongylus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal