A transcriptional activation domain of ATMYB2, a drought-inducible Arabidopsis Myb-related protein.
Trans-activation activity of ATMYB2, a drought-inducible Myb-related protein in Arabidopsis thaliana, was analyzed using a transient assay of Arabidopsis leaf protoplasts. ATMYB2 activated the transcription of a reporter gene from the MYB-binding site in a sequence-specific manner. Deletion of the C-terminal region of ATMYB2 reduced the trans-activation of the reporter gene, indicating that the acidic region at the C-terminus of ATMYB2 is required for transcriptional activation. The domain exchange analysis with the yeast GAL4 revealed that the C-terminal acidic region of ATMYB2 contains a sufficient domain for trans-activation. These results indicate that ATMYB2 acts as a transcriptional activator and that the C-terminal acidic region of ATMYB2 can function as a transcriptional activation domain.
Urao
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T
Biological Resources Division, Japan International Research Center for Agricultural Science (JIRCAS), Ibaraki, Japan.
Noji
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M
Yamaguchi-Shinozaki
K
K
Shinozaki
K
K
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics*; D029681:Arabidopsis Proteins; D004190:Disasters; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D010940:Plant Proteins / Q000235:genetics*; D011523:Protoplasts; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014867:Water / Q000378:metabolism*
D029681:Arabidopsis Proteins; C104175:MYB2 protein, Arabidopsis; D010940:Plant Proteins; D015534:Trans-Activators; D014867:Water