title: The 5' terminal oligopyrimidine tract confers translational control on TOP mRNAs in a cell type- and sequence context-dependent manner.
abstract: TOP mRNAs are vertebrate transcripts which contain a 5'terminal oligopyrimidine tract (5'TOP), encode for ribosomal proteins and elongation factors 1alpha and 2, and are candidates for growth-dependent translational control mediated through their 5'TOP. In the present study we show that elongation factor 2 (EF2) mRNA is translationally regulated in a growth-dependent manner in cells of hematopoietic origin, but not in any of three different non-hematopoietic cell lines studied. Human beta1-tubulin mRNA is a new member of the family which contains all the hallmarks of a typical TOP mRNA, yet its translation is refractory to growth arrest of any of the examined cell lines. Transfection experiments indicate that the first 29 and 53 nucleotides of the mRNAs encoding EF2 and beta1-tubulin, respectively, contain all the translational cis-regulatory elements sufficient for ubiquitously conferring growth-dependent translational control on a reporter mRNA. These results suggest that the distinct translational regulation of TOP mRNAs reflects downstream sequences which can override the regulatory features of the 5'TOP in a cell type-specific manner. This notion is further supported by the fact that mutations within the region immediately downstream of the 5'TOP of rpS16 mRNA confer onto the resulting transcripts growth-dependent translational control with a cell type specificity similar to that displayed by EF2 mRNA.
authors.affiliation: Department of Biochemistry, The Hebrew University Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Avni
authors.forename: Y
authors.initials: Y
authors.lastname: Biberman
authors.forename: O
authors.initials: O
authors.lastname: Meyuhas
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D002461:Cell Line, Transformed; D006224:Cricetinae; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009154:Mutation; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000235:genetics*; D014176:Protein Biosynthesis; D011743:Pyrimidines; D012333:RNA, Messenger / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012269:Ribosomal Proteins / Q000235:genetics; D014404:Tubulin / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D011743:Pyrimidines; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D014404:Tubulin; C023509:ribosomal protein S16