title: Absence of binding of human salivary glycoprotein to human gingival fibroblast-like cells in vitro.
abstract: The aim of this study was to determine whether human high molecular weight salivary glycoprotein binds in vitro to human gingival fibroblast-like cells. Primary monolayer cultures of 2 human gingival fibroblast-like cell lines were incubated with a high molecular weight fraction of salivary glycoprotein which expressed blood group A activity and glycoprotein-cell binding probed using an FITC-conjugated mouse monoclonal antibody to human blood group A antigen. Surface fluorescence of protein-treated cells was found to be no greater than that of untreated or serum-treated control cultures. As significant binding of salivary glycoprotein to gingival fibroblast-like cells does not occur in vitro, saliva-mediated inhibition of fibroblast attachment to hydroxyapatite is not dependent on specific ligand-lectin interactions.
authors.affiliation: Department of Orthodontics, School of Dentistry, University of Mainz, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Zentner
authors.forename: T G
authors.initials: TG
authors.lastname: Heaney
mesh_terms: D001790:Blood Physiological Phenomena; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D005881:Gingiva / Q000166:cytology; D006801:Humans; D009077:Mucins / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D012463:Saliva / Q000502:physiology; D012471:Salivary Proteins and Peptides / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D009077:Mucins; D012471:Salivary Proteins and Peptides