Labeling Salmonella live vaccine strains with the lux operon from Vibrio fischeri improves their detection and discrimination from wild type.
Genetic labeling of Salmonella live vaccine strains, ZoosaloralR H and TAD Salmonella vacR T, with part of or the entire lux operon of the marine bacterium Vibrio fischeri allows for detection and discrimination of these vaccine strains in the course of routine bacteriological culture procedures, without disturbance of such procedures and without the requirement for extra materials and working steps. The label is either plasmid coded or chromosomally integrated by a Tn5 transposon vector. One of the plasmid constructs contains a truncated lux operon raising the requirement for the exogenous addition of the aldehyde substrat of the luciferase to induce light production. All strains constructed produced light sufficiently bright to detect their colonies on the surface of a routinely used plate in the dark with the naked eye.
Beyer
W
W
University of Hohenheim, Institute of Environmental and Animal Hygiene, Stuttgart, Germany.
Böhm
R
R
D000818:Animals; D001431:Bacteriological Techniques; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015169:Colony Count, Microbial / Q000379:methods; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D005243:Feces / Q000382:microbiology; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009876:Operon / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic; D012475:Salmonella / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control; D014611:Vaccination; D014612:Vaccines / Q000235:genetics*; D014733:Vibrio / Q000235:genetics*
D004251:DNA Transposable Elements; D014612:Vaccines; D008156:Luciferases