title: Listeria monocytogenes Scott A transports glucose by high-affinity and low-affinity glucose transport systems.
abstract: Listeria monocytogenes transported glucose by a high-affinity phosphoenolpyruvate-dependent phosphotransferase system and a low-affinity proton motive force-mediated system. The low-affinity system (Km = 2.9 mM) was inhibited by 2-deoxyglucose and 6-deoxyglucose, whereas the high-affinity system (Km = 0.11 mM) was inhibited by 2-deoxyglucose and mannose but not 6-deoxyglucose. Cells and vesicles artificially energized with valinomycin transported glucose or 2-deoxyglucose at rates greater than those of de-energized cells, indicating that a membrane potential could drive uptake by the low-affinity system.
authors.affiliation: Department of Food Science and Technology, University of Nebraska-Lincoln 68583-0919, USA.
authors.forename: C
authors.initials: C
authors.lastname: Parker
authors.forename: R W
authors.initials: RW
authors.lastname: Hutkins
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D008089:Listeria monocytogenes / Q000378:metabolism* / Q000648:ultrastructure; D008358:Mannose / Q000494:pharmacology; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D018892:Proton-Motive Force; D011523:Protoplasts / Q000648:ultrastructure
chemical_list: D003847:Deoxyglucose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose; C037904:6-deoxyglucose; D008358:Mannose