title: B7.2 expressed by T cells does not induce CD28-mediated costimulatory activity but retains CTLA4 binding: implications for induction of antitumor immunity to T cell tumors.
abstract: The B7 family of costimulatory molecules provides the second signal necessary for activation of T cells. In the absence of the second signal, responding T cells become anergic. Although predominantly expressed on professional APCs, recent evidence shows that the B7 molecules are also expressed on T cells. To study the functions of B7 molecules on T cells, we transfected murine B7.1 (CD80) and B7.2 (CD86) cDNAs into the EL4 T cell thymoma cell line and examined the transfectants for their ability to costimulate T cell proliferation in vitro and to induce antitumor immunity in vivo. Here we show that although EL4-B7.1 cells costimulate T cells and induce tumor regression, EL4-B7.2 transfectants failed to costimulate T cell proliferation or induce tumor regression. To understand the cellular basis for this difference, we examined the binding of EL4-B7.1 and EL4-B7.2 to CTLA4 and CD28. Whereas EL4-B7.1 cells bound both CTLA4-Ig and CD28-Ig, EL4-B7.2 transfectants preferentially bound CTLA4-Ig, but not CD28-Ig. Similar binding data were obtained with freshly isolated murine T cells, which have been shown to constitutively express B7.2. Our data suggest, therefore, that B7.2 expressed on T cells may not costimulate but instead inhibit the T cell response by preferential binding to CTLA4.
authors.affiliation: Division of Hematologic Malignancies, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Greenfield
authors.forename: E
authors.initials: E
authors.lastname: Howard
authors.forename: T
authors.initials: T
authors.lastname: Paradis
authors.forename: K
authors.initials: K
authors.lastname: Nguyen
authors.forename: F
authors.initials: F
authors.lastname: Benazzo
authors.forename: P
authors.initials: P
authors.lastname: McLean
authors.forename: P
authors.initials: P
authors.lastname: Höllsberg
authors.forename: G
authors.initials: G
authors.lastname: Davis
authors.forename: D A
authors.initials: DA
authors.lastname: Hafler
authors.forename: A H
authors.initials: AH
authors.lastname: Sharpe
authors.forename: G J
authors.initials: GJ
authors.lastname: Freeman
authors.forename: V K
authors.initials: VK
authors.lastname: Kuchroo
mesh_terms: D000069594:Abatacept; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology*; D000943:Antigens, Differentiation / Q000378:metabolism*; D051940:B7-2 Antigen; D018106:CD28 Antigens / Q000378:metabolism / Q000502:physiology*; D060908:CTLA-4 Antigen; D002455:Cell Division; D005260:Female; D018796:Immunoconjugates; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011485:Protein Binding / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013945:Thymoma / Q000276:immunology* / Q000517:prevention & control; D013953:Thymus Neoplasms / Q000276:immunology* / Q000517:prevention & control; D014162:Transfection / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D051940:B7-2 Antigen; D018106:CD28 Antigens; D060908:CTLA-4 Antigen; C497965:Cd86 protein, mouse; C556707:Ctla4 protein, mouse; D018796:Immunoconjugates; D008562:Membrane Glycoproteins; D000069594:Abatacept