title: Simultaneous determination of acrolein, malonaldehyde and 4-hydroxy-2-nonenal produced from lipids oxidized with Fenton's reagent.
abstract: Ethyl linoleate, ethyl linolenate, ethyl arachidonate and cod liver oil were oxidized with Fenton's reagent. Acrolein, malonaldehyde and 4-hydroxynonenal formed were derivatized to N-methylpyrazoline, N-methylpyrazole and 5-(1'-hydroxyhexyl)-1-methyl-2-pyrazoline with N-methylhydrazine, respectively. The derivatives were simultaneously analysed by gas chromatograph equipped with a fused silica capillary column and a nitrogen-phosphorus detector. The maximum amounts of acrolein (9.7 +/- 2.11 nmol/ml) and malonaldehyde (61.18 +/- 6.51 nmol/ml) were formed from cod liver oil. The highest amount of 4-hydroxynonenal (6.83 +/- 0.53 nmol/ml) was produced from ethyl arathidonate.
authors.affiliation: Department of Environmental Toxicology, University of California, Davis 95616, USA.
authors.forename: T
authors.initials: T
authors.lastname: Miyake
authors.forename: T
authors.initials: T
authors.lastname: Shibamoto
mesh_terms: D000171:Acrolein / Q000032:analysis / Q000378:metabolism*; D000447:Aldehydes / Q000032:analysis / Q000378:metabolism*; D001095:Arachidonic Acids / Q000737:chemistry; D002849:Chromatography, Gas; D003060:Cod Liver Oil / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000032:analysis / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008041:Linoleic Acids / Q000737:chemistry; D008042:Linolenic Acids / Q000737:chemistry; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism*; D010084:Oxidation-Reduction; D012015:Reference Standards
chemical_list: D000447:Aldehydes; D001095:Arachidonic Acids; D003432:Cross-Linking Reagents; C045076:Fenton's reagent; D008041:Linoleic Acids; D008042:Linolenic Acids; C035723:ethyl arachidonate; D008315:Malondialdehyde; D000171:Acrolein; D003060:Cod Liver Oil; D006861:Hydrogen Peroxide; D007501:Iron; C027576:4-hydroxy-2-nonenal; C007678:ethyl linoleate; C039896:ethyl linolenate