Chloroplastic isoforms of DnaJ and GrpE in pea.
The folding and assembly of proteins in cells often requires the assistance of molecular chaperones such as the Hsp70 and Hsp60 heat shock proteins. Hsp70 chaperones cooperate with DnaJ and GrpE homologues to ensure a productive folding cycle. In this study we describe the gene for the first chloroplast localized DnaJ homologue and present evidence that the gene product is at least partially associated with the inner envelope membrane. Immunoblot analysis also provides evidence for the presence of a GrpE homologue in plastids.
Schlicher
T
T
Botanisches Institut, Universitat Kiel, Germany.
Soll
J
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry; D002736:Chloroplasts / Q000378:metabolism*; D017343:Genes, Plant; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry; D010944:Plants / Q000378:metabolism; D017510:Protein Folding; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; C068113:GrpE protein, Bacteria; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins