[Electron spin resonance determination of copper binding site on  Escherichia coli cell membrane].
The electron-spin relaxation of Escherichia coli cytoplasmic membrane strong binding Cu-centers was investigated by means of the microwave power saturation of the electronic spin resonance signal. It has been established, that copper centres in the strong binding sites of the cytoplasmic membrane E.coli may be represented in the following way: 1) isolate copper complexes with small speed of the spin-lattice relaxation; 2) isolate copper complexes with increased speed of spin-lattice relaxation by means of interaction with rapidly relaxation centres; 3) dipol-binding clasters; 4) ESR-nondetectable at T = 40 K, exchange-binding clasters, which cause increasing of the spin-lattice relaxation speed for isolate copper complexes.
Volodina
L A
LA
Burbaev
D Sh
DSh
Lebedev
V S
VS
Moroz
I A
IA
D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism*; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000378:metabolism*
D003300:Copper