title: [Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins].
abstract: Bacillus megaterium accumulates poly-(3-hydroxybutyrate) (PHB) as a reserve material in intracellular granules. In this work we described a method for the preparation of PHB granules from B. megaterium PV447 and the analysis by polyacrylamide gel electrophoresis of the associated proteins. By comparison with another species a function is proposed for some of these proteins.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Bueńos Aires, Argentina.
authors.forename: G J
authors.initials: GJ
authors.lastname: Vázquez
authors.forename: R
authors.initials: R
authors.lastname: Wieczoreck
authors.forename: A
authors.initials: A
authors.lastname: Steinbüchel
authors.forename: B S
authors.initials: BS
authors.lastname: Méndez
mesh_terms: D000217:Acyltransferases / Q000302:isolation & purification; D001410:Bacillus megaterium / Q000737:chemistry* / Q000201:enzymology / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002458:Cell Fractionation; D002844:Chromatium / Q000201:enzymology; D003594:Cytoplasmic Granules / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006885:Hydroxybutyrates / Q000032:analysis*; D037102:Lectins / Q000302:isolation & purification; D037121:Plant Lectins; D011091:Polyesters / Q000032:analysis*; D013045:Species Specificity; D000094122:Polyhydroxybutyrates
chemical_list: D000217:Acyltransferases; D001426:Bacterial Proteins; D006885:Hydroxybutyrates; D037102:Lectins; D037121:Plant Lectins; D011091:Polyesters; C008825:phasin; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C077225:poly(3-hydroxyalkanoic acid) synthase