Acute in vitro production of acylation stimulating protein in differentiated human adipocytes.
We have previously shown that in normolipidemic healthy adults, plasma acylation stimulating protein (ASP) increases postprandially and is produced in vitro by cultured differentiated human adipocytes. The present studies were undertaken to examine the influence of specific plasma components on endogenous ASP production in cultured human adipocytes. The results demonstrate that neither glucose nor fatty acids (over a wide range of concentrations) had any substantial effect on ASP production. Insulin increased ASP production up to 2-fold (208% +/- 18%, P < 0.01). However, the most profound increase in ASP was generated by the addition of chylomicrons to the cell culture medium. Chylomicrons (CHYLO) obtained from postprandial plasma increased ASP production in a time- and concentration-dependent manner, producing up to a 150-fold increase in ASP at the highest concentration of CHYLO tested (500 microg triacylglycerol/mL medium (P < 0.001)). By contrast, very low (VLDL), high (HDL), and low density lipoproteins (LDI) had only marginal effects. The effects on ASP parallelled the changes in adipocyte C3 secretion (the precursor protein of ASP). As with ASP, glucose, oleate, insulin, and hepatic lipoproteins (VLDL, LDL, and HDL) had little or no effect on C3 secretion. In contrast, CHYLO had an even greater effect on C3 secretion than on ASP generation. Finally, the effects of CHYLO on generation of ASP and C3 were not dependent on lipolysis of CHYLO by lipoprotein lipase (LPL). These results are consistent with the changes in plasma ASP seen postprandially, and suggests a role of ASP as a positive feedback regulator of triacylglycerol synthesis in adipose tissue.
Maslowska
M
M
McGill Unit for the Prevention of Cardiovascular Disease, Royal Victoria Hospital, Montreal, Quebec, Canada.
Scantlebury
T
T
Germinario
R
R
Cianflone
K
K
D017667:Adipocytes / Q000378:metabolism*; D000704:Analysis of Variance; D001798:Blood Proteins / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D015926:Complement C3a / Q000031:analogs & derivatives*; D005246:Feedback; D006801:Humans; D014280:Triglycerides / Q000378:metabolism*
D001798:Blood Proteins; D014280:Triglycerides; C044541:complement C3a, des-Arg-(77)-; D015926:Complement C3a