Expression of basic fibroblast growth factor and its mRNA in advanced uterine cervical cancers.
To know the potential of growth, invasion and metastasis of uterine cervical cancer cells associated with neovascularization, the expression of basic fibroblast growth factor (FGF) and its mRNA in uterine cervical cancers and normal uterine cervices as controls were determined by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction-Southern blot (RT-PCR-SB), respectively. Then, the relations between the expression and the histological grading and clinical staging in cervical cancers were analyzed. The levels of basic FGF and its mRNA were significantly higher in advanced primary uterine cervical cancers, regardless of histological type. Therefore, this status might contribute to the acceleration of growth, invasion, and metastasis with neovascularization in advanced uterine cervical cancers.
Fujimoto
J
J
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu City, Japan.
Ichigo
S
S
Hori
M
M
Hirose
R
R
Sakaguchi
H
H
Tamaya
T
T
D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology
D009363:Neoplasm Proteins; D016222:Fibroblast Growth Factor 2; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases