title: Isolation and characterization of the integration host factor genes of Pasteurella haemolytica.
abstract: Using a bacteriophage lambda complementation system in Escherichia coli, we cloned genes encoding subunits of the heterodimeric DNA binding/bending protein, integration host factor, from the bovine pathogen, Pasteurella haemolytica. Complementation of ihfA and ihfB mutations in E. coli demonstrated that the P. haemolytica gene products form functional heterologous heterodimers. The ihfA and ihfB genes encode polypeptides predicted to be 99 and 93 amino acids long, respectively, and are very similar to integration host factor subunits from other Gram-negative bacteria, although phylogenetic analysis indicated that the P. haemolytica sequences are distantly related to those from other bacteria. Most significant amino acid differences were restricted to the amino-terminal domains of the predicted peptides.
authors.affiliation: Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030, USA. sarahh@bcm.tmc.edu
authors.forename: S K
authors.initials: SK
authors.lastname: Highlander
authors.forename: O
authors.initials: O
authors.lastname: Garza
authors.forename: B J
authors.initials: BJ
authors.lastname: Brown
authors.forename: S
authors.initials: S
authors.lastname: Koby
authors.forename: A B
authors.initials: AB
authors.lastname: Oppenheim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D034982:Integration Host Factors; D016978:Mannheimia haemolytica / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D034982:Integration Host Factors