Increase of Cl- secretion induced by Kampo medicine (Japanese herbal medicine), Sai-rei-to, in Mongolian gerbil middle ear epithelium.
Sai-rei-to, a type of Kampo medicine (Japanese herbal medicine), has been shown to be clinically effective in treating patients with otitis media with effusion. The effect of Sai-rei-to on the ion transport of the middle ear surface epithelium cultured from the Mongolian gerbil was investigated by using an Ussing chamber. Application of Sai-rei-to to the mucosal bath but not the serosal bath induced an increase in the short-circuit current (I(SC)) in the basal state. The increase in I(SC) was almost completely inhibited by addition of diphenylamine-2-carboxylic acid but not by amiloride, indicating enhancement of Cl- secretion. On the basis of the lack of changes in the intracellular Ca2+ concentration and a sideness of action, the effect of Sai-rei-to on I(SC) is thought to be a direct and selective activation on the apical Cl- channel.
Ikeda
K
K
Department of Otorhinolaryngology, Tohoku University School of Medicine, Sendai, Japan.
Furukawa
M
M
Tanno
N
N
Yamaya
M
M
Takasaka
T
T
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D018118:Chloride Channels / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004432:Ear, Middle / Q000187:drug effects* / Q000378:metabolism; D017097:Electric Impedance; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005849:Gerbillinae; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D018118:Chloride Channels; D002712:Chlorides; D004365:Drugs, Chinese Herbal; D062367:ortho-Aminobenzoates; C080903:sairei-to; D000584:Amiloride; C001209:fenamic acid; D002118:Calcium