Discovery of a novel human G protein-coupled receptor gene (GPR25) located on chromosome 1.
We amplified human genomic DNA by the polymerase chain reaction (PCR) using oligonucleotides based on the primary sequence of the genes encoding the somatostatin receptors (SSTR) and the somatostatin-like receptor gene SLC-1. One resultant DNA fragment was used to screen a genomic DNA library resulting in the isolation of a gene, GPR25, encoding an additional member of the G protein-coupled receptor family (GPCR). GPR25 is intronless throughout its open reading frame (ORF) and encodes a protein of 360 amino acids. The receptor encoded by GPR25 shares highest identity to the receptor encoded by GPR15, angiotensin II type 1A receptor, and somatostatin receptor 5. Northern analysis found no transcripts expressed in liver or any of the 12 brain regions analyzed. Fluorescence in situ hybridization analysis localized GPR25 to chromosome 1q32.1.
Jung
B P
BP
Department of Pharmacology, University of Toronto, Ontario, Canada.
Nguyen
T
T
Kolakowski
L F
LF
Lynch
K R
KR
Heng
H H
HH
George
S R
SR
O'Dowd
B F
BF
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D017931:DNA Primers; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled; D017481:Receptors, Somatostatin / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; C103675:GPR25 protein, human; C103263:MCHR1 protein, human; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D017481:Receptors, Somatostatin; D019204:GTP-Binding Proteins