Removal after addition of NO-generating agents and 8-bromo-cyclic GMP causes morphological change of cultured cerebellar astrocytes: a new mode of NO action.
The effect of nitric oxide (NO) on cell morphology was investigated in primary cultures of cerebellar astrocytes. Although the addition of NO donors to the culture medium did not change glial morphology, their removal did, with the form of astrocytes changing from flat to process bearing. This 'removal effect' may be a new mode of NO action.
Tomita
A
A
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Yoshida
S
S
Yano
M
M
Kirino
Y
Y
Kawahara
S
S
Shimizu
H
H
D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects*; D006152:Cyclic GMP / Q000494:pharmacology*; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D009599:Nitroprusside; D009569:Nitric Oxide; D006152:Cyclic GMP