title: Cationic lipid-mediated NGF gene transfection increases neurofilament phosphorylation.
abstract: We examined the effect of cationic lipid-mediated gene transfection of nerve growth factor (NGF) in primary septo-hippocampal cell cultures. Rat NGF cDNA was subcloned into a pUC19-based plasmid containing a CMV promoter. Two days after NGF gene transfection in primary cell cultures, ELISA confirmed increases in NGF protein secretion from transfected cells. To study the biological effect of cationic lipid-mediated NGF gene transfection, we analyzed the amount of neurofilament protein from NGF-transfected cell cultures. Western blot and immunohistochemical analyses detected significant increases in the phosphorylated form of neurofilament proteins in the cultures after cationic lipid-mediated NGF cDNA transfection. Cationic lipid-mediated NGF cDNA transfection did rot cause significant changes in the total amount of neurofilament protein. Our studies suggest that cationic lipid-mediated NGF gene transfection can increase neurofilament phosphorylation but not total neurofilament protein.
authors.affiliation: Department of Neurosurgery, University of Texas Health Sciences Center at Houston 77030, USA.
authors.forename: K
authors.initials: K
authors.lastname: Yang
authors.forename: J J
authors.initials: JJ
authors.lastname: Xue
authors.forename: Y H
authors.initials: YH
authors.lastname: Qiu
authors.forename: J R
authors.initials: JR
authors.lastname: Perez-Polo
authors.forename: J
authors.initials: J
authors.lastname: Whitson
authors.forename: F
authors.initials: F
authors.lastname: Faustinella
authors.forename: A
authors.initials: A
authors.lastname: Kampfl
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Iwamoto
authors.forename: X S
authors.initials: XS
authors.lastname: Mu
authors.forename: G
authors.initials: G
authors.lastname: Clifton
authors.forename: R L
authors.initials: RL
authors.lastname: Hayes
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007150:Immunohistochemistry; D008055:Lipids / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000235:genetics* / Q000378:metabolism; D016900:Neurofilament Proteins / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection / Q000187:drug effects*
chemical_list: D008055:Lipids; D009414:Nerve Growth Factors; D016900:Neurofilament Proteins