title: [Purification of EcoO44I restriction endonuclease in Escherichia coli O44 isolated from an affected human].
abstract: A restriction endonuclases (ENase) designated EcoO44I was purified without non specific nucleases from enteropathogenic Escherichia coli O44 Hiromi strain of affected human origin. The yield was 1, 100 units/g of wet cells. The EcoO44I ENase recognized and cleaved the specific sequence of 5'-GGTCTC-3' (1/5) as was the case with Eco31I or BsaI ENase. Because of the stability and high yield, EcoO44I would be useful for recombinant DNA technology after isolation of EcoO44-positive, avirulent mutant strains of E. coli O44 Hiromi.
authors.forename: M
authors.initials: M
authors.lastname: Miyahara
authors.forename: N
authors.initials: N
authors.lastname: Shinohara
authors.forename: K
authors.initials: K
authors.lastname: Mise
mesh_terms: D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans
chemical_list: D004262:DNA Restriction Enzymes