title: Rapid gene transfer into cultured hippocampal neurons and acute hippocampal slices using adenoviral vectors.
abstract: Primary cultures of hippocampal neurons were infected with an adenovirus coding for beta-galactosidase. Expression could be detected as early as 4 h after infection and steadily increased to high levels at 24 h without evidence for a functional impairment of the infected neurons. Similarly, adenovirus-mediated gene transfer into acute hippocampal slices was detectable 4 h after infection and could be localized to discrete areas of the CA1 region by microinjection of the virus stock solution. Infected slices were still suitable for electrophysiological experiments.
authors.affiliation: Abteilung Neurochemie, Max-Planck-Institut für Psychiatrie, Martinsried, Germany. griesbec@alf.biochem.mpg.de
authors.forename: O
authors.initials: O
authors.lastname: Griesbeck
authors.forename: M
authors.initials: M
authors.lastname: Korte
authors.forename: C
authors.initials: C
authors.lastname: Gravel
authors.forename: T
authors.initials: T
authors.lastname: Bonhoeffer
authors.forename: H
authors.initials: H
authors.lastname: Thoenen
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D006624:Hippocampus / Q000648:ultrastructure / Q000821:virology*; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001616:beta-Galactosidase