Recovery of waterborne oocysts of Cryptosporidium from water samples by the membrane-filter dissolution method.
The cellulose-acetate membrane (CAM)-filter dissolution method implemented into a Millipore Glass Microanalysis system was used for recovery of Cryptosporidium parvum oocysts seeded into 25 l of drinking water in polyethylene carboy aspirator bottles. CAM-entrapped oocysts were detected by immunofluorescence microscopy. From 65 to 94 oocysts/l (mean 75 oocysts/l), 34.7% overall of the inoculated oocysts, were unrecovered as determined after the water had been drained from the bottle, rinsed with 1 l of eluting fluid (EF), and CAM-filtered. Efficiency rates of oocyst recovery ranged from 24.0% to 64.0% (mean 44.1%), without the use of EF and from 72.1% to 82.3% (mean 78.8%) when EF was used. To ensure a high recovery efficiency of Cryptosporidium oocysts from sampled water by the CAM-filter dissolution method, it is recommended that 1 l of EF per 25 l of water be used.
Graczyk
T K
TK
Department of Molecular Microbiology and Immunology, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205, USA.
Cranfield
M R
MR
Fayer
R
R
D000818:Animals; D002417:Cattle; D003457:Cryptosporidiosis / Q000635:transmission; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D005374:Filtration; D006801:Humans; D014867:Water / Q000469:parasitology*; D014881:Water Supply; D015053:Zygote
D014867:Water