title: Vanadium affects vacuolation and phosphate metabolism in Hansenula polymorpha.
abstract: The yeast Hansenula polymorpha is able to grow on vanadate concentrations that are toxic to other organisms. Transmission electron microscopy analysis showed that H. polymorpha cells growing on a vanadate-containing medium undergo a significant increase in cell vacuolation and a thickening of the cell wall; the presence of small cytoplasmic vesicles and an increase in cristae at the level of the plasma membrane were also observed. These ultrastructural modifications were accompanied by a change in the intracellular polyphosphate level, as shown by in vivo 31P-NMR. The involvement of these observed changes in vanadium detoxification is discussed.
authors.affiliation: Dipartimento di Biotecnologie Agrarie ed Ambientali, Università degli studi di Ancona, Italy. ilaria@anvax1.cineca.it
authors.forename: I
authors.initials: I
authors.lastname: Mannazzu
authors.forename: E
authors.initials: E
authors.lastname: Guerra
authors.forename: R
authors.initials: R
authors.lastname: Strabbioli
authors.forename: A
authors.initials: A
authors.lastname: Masia
authors.forename: G B
authors.initials: GB
authors.lastname: Maestrale
authors.forename: M A
authors.initials: MA
authors.lastname: Zoroddu
authors.forename: F
authors.initials: F
authors.lastname: Fatichenti
mesh_terms: D002455:Cell Division / Q000187:drug effects; D004352:Drug Resistance, Microbial; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D010710:Phosphates / Q000378:metabolism*; D010759:Phosphorus Isotopes; D010843:Pichia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014617:Vacuoles / Q000187:drug effects* / Q000648:ultrastructure; D014638:Vanadates / Q000494:pharmacology; D014639:Vanadium / Q000494:pharmacology*
chemical_list: D010710:Phosphates; D010759:Phosphorus Isotopes; D014639:Vanadium; D014638:Vanadates