Isolation and characterization of the carotenoid biosynthesis genes of Flavobacterium sp. strain R1534.
The Gram-negative bacterium Flavobacterium sp. strain R1534 is a natural producer of zeaxanthin. A 14 kb genomic DNA fragment of this organism has been cloned and a 5.1 kb piece containing the carotenoid biosynthesis genes sequenced. The carotenoid biosynthesis cluster consists of five genes arranged in at least two operons. The five genes are necessary and sufficient for the synthesis of zeaxanthin. The encoded proteins have significant homology to the crtE, crtB, crtY, crtI and crtZ gene products of other carotenogenic organisms. Biochemical assignment of the individual gene products was done by HPLC analysis of the carotenoid accumulation in Escherichia coli host strains transformed with plasmids carrying deletions of the Flavobacterium sp. strain R1534 carotenoid biosynthesis cluster.
Pasamontes
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F. Hoffmann-La Roche Ltd., Vitamins and Fine Chemicals Division, Basel, Switzerland. luis.pasamontes@roche.com
Hug
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Tessier
M
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Hohmann
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Schierle
J
J
van Loon
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AP
D019883:Alkyl and Aryl Transferases; D015139:Blotting, Southern; D002338:Carotenoids / Q000235:genetics* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D001330:Electronic Data Processing; D004926:Escherichia coli / Q000235:genetics; D005417:Flavobacterium / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D016680:Genome, Bacterial; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D019753:Intramolecular Lyases; D007535:Isomerases / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D009876:Operon / Q000235:genetics; D010802:Phylogeny; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014166:Transferases / Q000235:genetics; D014170:Transformation, Genetic; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics
D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D002338:Carotenoids; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D007535:Isomerases; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase