[The determination of the group classification of sperm by the ABO system by means of the immunofluorescence reaction in a quantitative modification].
Differentiating lysis with proteinase K helps purify the preparations from stains on material evidences and thus permits the detection of more spermatozoa. Quantitative immunofluorescence and the Stat software helps objectively assess the results of the test by mathematical processing and more reliably detect this or that AB0 antigen in the spermatozoa.
Sidorov
V L
VL
Gavshinskaia
N S
NS
Maiatskaia
M V
MV
Afinogenov
I L
IL
Smirnov
V A
VA
D000017:ABO Blood-Group System / Q000032:analysis*; D002021:Buffers; D019286:Endopeptidase K; D005455:Fluorescent Antibody Technique / Q000706:statistics & numerical data; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008297:Male; D013094:Spermatozoa / Q000145:classification* / Q000276:immunology*
D000017:ABO Blood-Group System; D002021:Buffers; D007202:Indicators and Reagents; D019286:Endopeptidase K