Maturation of respiratory syncytial virus within HEp-2 cell cytoplasm.
Electron microscopy of HEp-2 cells infected with respiratory syncytial virus (RSV) strain Long revealed the maturation of RSV on an ultrastructural level. The results showed that the virus maturated by two different pathways. In one of them, the virus assembled and matured before reaching the plasma membrane on the internal vesicle membrane within cytoplasm. The mature virus was delivered to the plasma membrane and to the extracellular space most likely by the transport vesicles and exocytosis. In the other pathway, the virus matured on the plasma membrane as described with other members of the family Paramyxoviridae. Using monoclonal antibodies (MoAbs), we localized viral nucleoprotein (NP) and envelope proteins in cytoplasm by immunoelectron microscopy (IEM).
Arslańagic
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E
Department of Virology 1, National Institute of Health, Tokyo, Japan.
Matsumoto
M
M
Suzuki
K
K
Nerome
K
K
Tsutsumi
H
H
Hung
T
T
D000911:Antibodies, Monoclonal; D003593:Cytoplasm / Q000821:virology; D015707:HN Protein; D006801:Humans; D016253:Microscopy, Immunoelectron; D019251:Nucleocapsid / Q000378:metabolism / Q000648:ultrastructure; D012136:Respiratory Syncytial Viruses / Q000254:growth & development* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism / Q000648:ultrastructure; D014771:Virion / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D015707:HN Protein; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G