Syntenic assignment of dopamine tautomerase (DCT) to bovine chromosome 12.
Heterologous primers were used to amplify an exon and intron-containing segment of the bovine homologue of the human dopachrome tautomerase gene. After confirmation of homology by sequence analysis (exon sequence similarity greater than 90%), bovine-specific primers were developed for synteny mapping purposes. The dopachrome tautomerase gene was assigned to bovine chromosome 12 (BTA12) with 97% concordance to the coagulation factor 10 locus. Together with previous synteny mapping of bovine chromosome 12 genes, fms-related tyrosine kinase, esterase D and 5-hydroxytryptamine receptor 2, this assignment further indicates conservation between human chromosome 13q and bovine chromosome 12.
Sun
H S
HS
Department of Animal Sciences, University of Wisconsin, Madison, California 53706, USA.
Womack
J E
JE
Kirkpatrick
B W
BW
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000378:metabolism*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D017124:Conserved Sequence; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000235:genetics*; D051379:Mice; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D017931:DNA Primers; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase