title: Simple extractive derivatization of methamphetamine and its metabolites in biological materials with Extrelut columns for their GC-MS determination.
abstract: A simple, extractive heptafluoro-n-butyrylation with Extrelut columns was devised to simultaneously measure methamphetamine (MAMP), amphetamine (AMP), 4-hydroxymethamphetamine (HMAMP), and 4-hydroxyamphetamine (HAMP) in biological materials by gas chromatography-mass spectrometry (GC-MS) using 4-methoxymethamphetamine-d5 as the internal standard. Human urine, human whole blood, and porcine skeletal muscle spiked with the stimulant standards were used for evaluating the method. After deproteinization and adjustment of the pH to 12.6, the sample was applied to an Extrelut column. Using the present method, AMP, MAMP, and HMAMP could be determined in an actual forensic case study.
authors.affiliation: Department of Forensic Medicine, Fukuoka University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: S
authors.initials: S
authors.lastname: Kashimura
authors.forename: Y
authors.initials: Y
authors.lastname: Hieda
authors.forename: M
authors.initials: M
authors.lastname: Kageura
mesh_terms: D000661:Amphetamine / Q000032:analysis*; D000818:Animals; D000697:Central Nervous System Stimulants / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000737:chemistry; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods; D013552:Swine; D010136:p-Hydroxyamphetamine / Q000032:analysis*
chemical_list: D000697:Central Nervous System Stimulants; D008694:Methamphetamine; C005877:pholedrine; D000661:Amphetamine; D010136:p-Hydroxyamphetamine