A rapid plate assay for screening L-asparaginase producing micro-organisms.
A pH and dye-based fast procedure for screening L-asparaginase producing micro-organisms is reported. The procedure is suitable for bacterial and fungal screening. The results are obtained within 24 and 48 h for bacteria and fungi, respectively. The results correlate with quantitative estimations in culture broths.
Gulati
R
R
Department of Microbiology, University of Delhi South Campus, India.
Saxena
R K
RK
Gupta
R
R
D001215:Asparaginase / Q000378:metabolism*; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification*; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D001215:Asparaginase