Evaluation of HBsAg carrier rate in acute viral hepatitis and high risk individuals using RPHA and ELISA.
A total of 600 individuals including 500 cases of hepatitis and 100 individuals at high risk for developing hepatitis were screened for hepatitis B surface antigen (HBsAg) using reverse passive haemagglutination (RPHA) and enzyme linked immunosorbent assay (ELISA). HBsAg carrier rate in clinically diagnosed cases of hepatitis was 38% and 32.4% by RPHA and ELISA respectively. In high risk individuals, the carrier rate was 14% by RPHA and 11% by ELISA. Taking ELISA as gold standard, RPHA showed 5.33% false positivity and 0.33% false negativity. The over all correlation between RPHA and ELISA was to the tune of 82.66%.
Korgaonkar
A
A
Department of Microbiology, Goa Medical College, Bamboli.
Verenkar
M P
MP
Savio
R
R
Pinto
M J
MJ
Singh
I
I
D000208:Acute Disease; D002353:Carrier State / Q000453:epidemiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006386:Hemagglutination Tests / Q000379:methods; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006525:Hepatitis, Viral, Human / Q000453:epidemiology*; D006801:Humans
D006514:Hepatitis B Surface Antigens