title: Oligodendrocytes isolated from adult pig brain synthesise and release prostaglandins.
abstract: The synthesis and release of prostaglandins have been studied in oligodendrocytes isolated from adult pig brain. Radioactively labelled arachidonic acid and di-homo-y-linoleic acid were offered as precursors and their incorporation into individual phospholipids followed. Most of the labelling was incorporated into phosphatidylinositol, which may serve as a major source for precursors of the eicosanoid biosynthesis. Oligodendrocytes are capable of synthesising and releasing prostaglandins of the E- and F-series in vitro. Cyclooxygenase, the principal enzyme for prostaglandin synthesis, was localised in oligodendrocytes immunocytochemically by using a double-labelling technique which identified the oligodendrocytes via anti-galactosylcerebroside. The production and release of oligodendroglial prostaglandins indicate that oligodendrocytes themselves can modulate immune-mediated processes in the white matter of the central nervous system. Furthermore, prostaglandins may also play a role during remyelination.
authors.affiliation: Max Planck Institute for Experimental Medicine, Hermann-Rein-Strasse 3, D-37085 Göttingen, Germany.
authors.forename: H H
authors.initials: HH
authors.lastname: Althaus
authors.forename: C
authors.initials: C
authors.lastname: Siepl
mesh_terms: D015117:5,8,11,14-Eicosatetraynoic Acid / Q000493:pharmacokinetics; D015126:8,11,14-Eicosatrienoic Acid / Q000493:pharmacokinetics; D000527:Alprostadil / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011458:Prostaglandins E / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis*; D013552:Swine
chemical_list: D010743:Phospholipids; D011458:Prostaglandins E; D011460:Prostaglandins F; D015117:5,8,11,14-Eicosatetraynoic Acid; D000242:Cyclic AMP; D000527:Alprostadil; D015126:8,11,14-Eicosatrienoic Acid