[Oocysts of Cryptosporidium sp in feces: comparison of the modified Kinyoun and Heine methods].
The diagnosis of intestinal infection by Cryptosporidium sp is crucial today; with the progression of the AIDS epidemic, many cases of cryptosporidiosis have appeared in this setting and in other immunodeficiency diseases. We compared the advantages and disadvantages of Heine's method and modified Kinyoun's method in the following parameters: morphology of the parasite, quantitation of cysts, stability of the staining characteristics timewise on the slides and time spend at staining. All positive fecal smears were obtained from patients with AIDS. The sensitivity of these two techniques was the same. The choice should be made by the best aspects of each method. Heine's was better for quantitation of the cysts and was faster. Kinyoun's was better for conserving the stained smear.
Amato Neto
V
V
Laboratório de Investigação Médica-Parasitologia, Universidade de São Paulo.
Braz
L M
LM
Di Pietro
A O
AO
Módolo
J R
JR
D017088:AIDS-Related Opportunistic Infections / Q000469:parasitology*; D000818:Animals; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D010274:Parasitology / Q000379:methods