title: Simultaneous display of different peptides on the surface of filamentous bacteriophage.
abstract: We have developed a new system for producing hybrid virions of filamentous bacteriophage fd simultaneously displaying two different peptides by infecting cells harbouring a plasmid containing a modified gene VIII with an engineered bacteriophage carrying a second and different copy of a modified gene VIII. The simultaneous display of different peptides has many potential applications in exploring the immune response and studying protein-protein interaction.
authors.affiliation: Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK.
authors.forename: P
authors.initials: P
authors.lastname: Malik
authors.forename: R N
authors.initials: RN
authors.lastname: Perham
mesh_terms: D000595:Amino Acid Sequence; D017912:Inovirus / Q000737:chemistry* / Q000235:genetics; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis* / Q000235:genetics; D015202:Protein Engineering; D014771:Virion / Q000737:chemistry / Q000235:genetics
chemical_list: D008565:Membrane Proteins; D010446:Peptide Fragments