Cloning unmodified PCR products using engineered XcmI restriction sites in a portable cassette.
Testori
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A
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA.
Sollitti
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D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D004275:DNA, Ribosomal; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli; D005822:Genetic Vectors; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping
D017931:DNA Primers; D004275:DNA, Ribosomal; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA; C084267:endodeoxyribonuclease XcmI; D015252:Deoxyribonucleases, Type II Site-Specific