title: Rapid and efficient one-tube PCR-based mutagenesis method.
authors.affiliation: Microbiology Department, Temple University Medical School, Philadelphia, PA, USA.
authors.forename: V
authors.initials: V
authors.lastname: Picard
authors.forename: S C
authors.initials: SC
authors.lastname: Bock
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000138:chemical synthesis*; D004262:DNA Restriction Enzymes; D004259:DNA-Directed DNA Polymerase; D004926:Escherichia coli; D007202:Indicators and Reagents; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D019914:Taq Polymerase; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D007202:Indicators and Reagents; C072146:Pfu DNA polymerase; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes