title: Anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence.
abstract: Aiming to assess the presence of selected anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis. 25 central and lateral upper incisors presenting with radiographic evidence of chronic apical periodontitis were studied. The pulp chamber was opened under aseptic conditions and samples of the root canal content were collected with sterile absorbent paper points, which were placed and dispersed in test tubes containing reduced transport medium RTT. Aliquots were dried on glass slides and stained by indirect immunofluorescence for detection of Actinomyces viscosus, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia. The results showed a positive indirect immunofluorescence reaction in 24 of the 25 samples. Fourteen were positive for the specie Actinomyces viscosus, 12 for Prevotella intermedia, 10 for Fusobacterium nucleatum and 4 for Porphyromonas gingivalis. A semiquantitative assay was easily implemented for assessment of degree of infection by the organisms in individual cases.
authors.affiliation: Department of Pediatric Dentistry, Ribeiräo Preto School of Pharmacy USP, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Assed
authors.forename: I Y
authors.initials: IY
authors.lastname: Ito
authors.forename: M R
authors.initials: MR
authors.lastname: Leonardo
authors.forename: L A
authors.initials: LA
authors.lastname: Silva
authors.forename: D E
authors.initials: DE
authors.lastname: Lopatin
mesh_terms: D016924:Actinomyces viscosus / Q000302:isolation & purification; D000328:Adult; D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D002908:Chronic Disease; D003786:Dental Pulp Cavity / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D006801:Humans; D010485:Periapical Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000302:isolation & purification; D014092:Tooth Root / Q000382:microbiology