A pseudorabies virus mutant with deletions in the latency and early protein O genes: replication, virulence, and immunity in neonatal piglets.
The pathogenicity of a double mutant of pseudorabies virus (PRV) with deletions in the latency gene and the early protein O gene was examined. In comparison to the parent Indiana-Funkhauser virus, the ability of this mutant to replicate and to cause disease in piglets is greatly reduced. At an infection dose that caused no clinical signs in 5-day-old neonatal piglets, this mutant was capable of eliciting solid protective immunity against a lethal PRV challenge. Thus, the double-gene deletion attenuates PRV but does not affect its immunogenicity. These features may be desirable for inclusion into future PRV vaccines.
Wesley
R D
RD
Virology Swine Research Unit, National Animal Disease Center, USDA, Ames, IA 50010, USA.
Cheung
A K
AK
D000818:Animals; D000831:Animals, Newborn; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D011557:Pseudorabies / Q000276:immunology* / Q000503:physiopathology; D015183:Restriction Mapping; D017384:Sequence Deletion; D013552:Swine; D014764:Viral Proteins / Q000235:genetics*; D014765:Viral Vaccines; D014774:Virulence; D014779:Virus Replication; D017201:Virus Shedding
D014764:Viral Proteins; D014765:Viral Vaccines; C070914:early protein O, Pseudorabies virus