title: Cytokine levels after open and laparoscopic cholecystectomy.
abstract: A prospective study of serum cytokine levels was performed in patients randomly assigned to undergo either laparoscopic cholecystectomy (LC) or open cholecystectomy (OC). The kinetics of serum interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10), and cortisol were studied in both groups of patients. Cytokine and cortisol levels were measured in serum samples from patients who underwent either LC (n = 14) or OC (n = 14) using enzyme-linked immunosorbent assay and radioimmunoassay, respectively. Serum samples were obtained 24 h before surgery and 24 h and 7 days after surgery. IL-6 levels differed significantly (p < 0.05) between the LC and OC groups. IL-1 beta, IL-10, TNF-alpha and cortisol levels showed no significant differences (p > 0.05). Kinetic studies of IL-6, IL-1 beta and TNF-alpha levels revealed them to behave similarly, 24 h after surgery the levels of these cytokines were higher than those 24 h before surgery. These levels normalized by 7 days after surgery. Cytokine concentrations were always higher in the OC group than in the LC group. IL-1 beta and IL-10 levels were the most stable in both groups, though cortisol levels were also fairly stable.
authors.affiliation: Department of Morphological Sciences and Surgery (Surgical Research Laboratory), Faculty of Medicine, University of Alcalá, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Bellón
authors.forename: L
authors.initials: L
authors.lastname: Manzano
authors.forename: L
authors.initials: L
authors.lastname: Bernardos
authors.forename: N
authors.initials: N
authors.lastname: Ga-Honduvilla
authors.forename: A
authors.initials: A
authors.lastname: Larrad
authors.forename: J
authors.initials: J
authors.lastname: Buján
authors.forename: M
authors.initials: M
authors.lastname: Alvarez-Mon
mesh_terms: D000328:Adult; D002763:Cholecystectomy / Q000379:methods; D017081:Cholecystectomy, Laparoscopic / Q000379:methods*; D002769:Cholelithiasis / Q000097:blood* / Q000601:surgery; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D011446:Prospective Studies; D011863:Radioimmunoassay
chemical_list: D016207:Cytokines; D006854:Hydrocortisone