A chromosome-specific microdissected library increases marker density on bovine chromosome 1.
Genetic resolution of bovine chromosome 1 (BTA1) linkage group was significantly increased by screening for microsatellite clones a microdissected library constructed from a bovine cell line carrying a t(1;29) translocation. Eighty-five percent of the microsatellites (ms) (46/54) identified were informative in the USDA/MARC mapping population, and 96% of these ms (44/46) linked to BTA1 (LOD > 3.0). When merged with 40 existing BTA1 markers the genetic map spanned 153.8 cM (sex-averaged interval, 1.9 cM). The fourfold improvement in marker density of BTA1 provides a genetic map that enhances mapping of quantitative trait loci and implementation of marker assisted selection.
Sonstegard
T S
TS
Abel Ponce de León
F
F
Beattie
C W
CW
Kappes
S M
SM
D000818:Animals; D002417:Cattle; D002875:Chromosomes / Q000235:genetics*; D005819:Genetic Markers; D015698:Genomic Library
D005819:Genetic Markers