Optical tweezers-based immunosensor detects femtomolar concentrations of antigens.
We used optical tweezers (optical trapping technology) to measure the force required to separate antigen-antibody bonds. Under competitive-binding conditions, we used the force determination to detect and measure protein antigen concentrations as small as 1 fmol/L (10(-15) mol/L).
Helmerson
K
K
Atomic Physics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Kishore
R
R
Phillips
W D
WD
Weetall
H H
HH
D000906:Antibodies / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000378:metabolism; D000941:Antigens / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D007834:Lasers; D008832:Microchemistry; D008863:Microspheres; D055095:Optics and Photonics; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens; D012710:Serum Albumin, Bovine