A case of foodborne listeriosis in Sweden.
A 70-year-old woman fell seriously ill overnight with meningitis and was admitted to hospital. Cerebrospinal fluid culture yielded Listeria monocytogenes. One of the first problems in solving a human case of listeriosis suspected to be foodborne is to find the foods likely to have been transmitting L. monocytogenes. Two enrichment procedures and a direct plating procedure were used for isolation of the bacteria from different food items collected from the patient's refrigerator, local retail store and producer. Samples of vacuum-packed products of sliced pork brawn, sliced cooked medwurst and berliner wurst of the same brand harboured L. monocytogenes. Serotyping and restriction enzyme analysis (REA) with pulsed-field gel electrophoresis (PFGE) were used to characterize and compare 41 isolates, including the human strain. At least three clones were present in the foods investigated, and one of these was identical to the human clone. This clone was present in samples of medwurst from the patient's refrigerator and the local retail store. This is, to our knowledge, the first proven foodborne case of listeriosis reported in Sweden.
Loncarevic
S
S
Department of Food Hygiene, Faculty of Vetennary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden. Semir.Loncarevic@Lmhyg.shu.se
Danielsson-Tham
M L
ML
Mårtensson
L
L
Ringnér
A
A
Runehagen
A
A
Tham
W
W
D000368:Aged; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008088:Listeriosis / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000635:transmission*; D008460:Meat / Q000382:microbiology*; D016920:Meningitis, Bacterial / Q000175:diagnosis*; D017720:Molecular Epidemiology; D012150:Polymorphism, Restriction Fragment Length; D000091402:Prohibitins; D011635:Public Health Administration; D012703:Serotyping; D013548:Sweden
D003470:Culture Media; D004269:DNA, Bacterial; C000717331:PHB2 protein, human; D000091402:Prohibitins