A novel tetracycline-dependent expression vector with low basal expression and potent regulatory properties in various mammalian cell lines.
The tetracycline-dependent expression system has gained increasing popularity for the expression of any gene of interest. Careful choice of the expression vector has been suggested to exploit the full potential of this system. A novel tetracycline-sensitive expression vector based on a modified mouse mammary tumor virus promoter achieved considerably improved regulatory properties in a series of cell lines tested under transient and stable conditions. Therefore, the applicability of the tetracycline-dependent expression system can be largely enhanced by careful adaptation of the expression vector to the host cell line.
Hoffmann
A
A
Max Planck Institute of Psychiatry, Molecular Neurobiology, Kraepelinstrasse 2-10, D-80804 Munich, Germany.
Villalba
M
M
Journot
L
L
Spengler
D
D
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D018374:LLC-PK1 Cells; D008322:Mammals; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic; D013552:Swine; D013752:Tetracycline / Q000494:pharmacology*; D014162:Transfection
D013752:Tetracycline