Rapid sequence-independent cellular response to oligodeoxynucleotides.
The presence of receptors for oligodeoxynucleotides (OdN) on the surface of L929 cells has previously been described. To study the possible coupling of the receptor to cellular signal transducing systems, the effect of phosphodiester OdN of different sequences on cellular phospholipase C and protein kinase C (PKC) activities in L929 fibroblasts was studied. Treatment of cells with OdN induced an increase in 32P labeling of phosphatidic acid which was accompanied by a gradual decrease in diacylglycerol. These effects seem to be independent of the OdN sequence. PKC activity in membranes isolated from OdN-treated cells was found to be lower than that in membranes of control cells. SDS-PAGE of the 32P-labeled cellular proteins revealed that OdN treatment caused a decrease in phosphorylation of the 26 and 73 kDa cellular proteins in the cells.
Balakireva
L A
LA
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Academy of Sciences, Russian Federation. lis@genome.nsk.su
Levashova
Z B
ZB
Chroboczek
J
J
Vlassov
V V
VV
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010712:Phosphatidic Acids / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism*
D004075:Diglycerides; D009838:Oligodeoxyribonucleotides; D010712:Phosphatidic Acids; D011956:Receptors, Cell Surface; D011493:Protein Kinase C; D010738:Type C Phospholipases