CGP 39653 binding in the chick CNS after NMDA receptor antagonist treatment.
To analyze the affect of blocking neuronal activity on NMDA receptor levels during development, we have injected chick embryos with the competitive NMDA receptor antagonist NPC 12626 from E17 to E19. Brains from drug-treated (n = 7) and control (n = 6) embryos (E20) were processed for receptor autoradiography using the NMDA competitive antagonist [3H]CGP 39653. NPC 12626 treatment caused a significant 24 to 46% increase in [3H]CGP 39653 binding in both the forebrain and cerebellar cortex. The results support the hypothesis that NMDA receptor levels are regulated by activity-dependent mechanisms.
White
B H
BH
Department of Pediatrics, Georgetown University Medical Center, Washington, DC, USA.
Vogel
M W
MW
D015763:2-Amino-5-phosphonovalerate / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry / Q000187:drug effects; D002490:Central Nervous System / Q000187:drug effects / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002642:Chick Embryo; D018691:Excitatory Amino Acid Antagonists / Q000378:metabolism*; D009068:Movement / Q000187:drug effects; D016548:Prosencephalon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011689:Purkinje Cells / Q000187:drug effects / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
D018691:Excitatory Amino Acid Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; C069052:CGP 39653; D015763:2-Amino-5-phosphonovalerate