Efficacy of hydroxyapatite ceramic as a carrier for recombinant human bone morphogenetic protein.
In this study, we investigated the efficacy of hydroxyapatite ceramic (HAP) as a carrier of bone morphogenetic protein (BMP) using porous HAP pellets artificially fabricated from limestone. After treatment with recombinant human BMP-2, the pellets were inserted beneath the cranial periosteum of rabbits, and the degree of osteogenesis was examined histologically. The degree of osteogenesis was also evaluated using image-analyzing procedures. Results showed that extensive bone formation had occurred around the pellets 3 weeks after insertion in the group that received pellets treated with recombinant human BMP alone as well as in the group that received recombinant human BMP in addition to type I collagen-treated HAP pellets. Subsequently, osteogenesis within the pellets slowly progressed over time, and by 9 weeks after insertion most of the pellet pores in both groups were filled with newly generated bone. The recombinant human BMP-collagen group, however, exhibited a significantly greater bone induction. These results indicated that if recombinant human BMP is used clinically in the future, artificially fabricated HAP would be a suitable carrier.
Ono
I
I
Division of Plastic and Reconstructive Surgery, Fukushima Medical College, Japan.
Gunji
H
H
Kaneko
F
F
Saito
T
T
Kuboki
Y
Y
D000704:Analysis of Variance; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D003094:Collagen / Q000008:administration & dosage; D004337:Drug Carriers; D006801:Humans; D006882:Hydroxyapatites; D010012:Osteogenesis / Q000187:drug effects*; D010521:Periosteum / Q000601:surgery; D019736:Prostheses and Implants; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage; D012535:Scalp / Q000601:surgery; D012886:Skull
D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D006882:Hydroxyapatites; D011994:Recombinant Proteins; D003094:Collagen