Genomic structure and promoter characterization of the human ACTH receptor gene.
One kb of the 5'-flanking region of the human ACTH receptor gene was isolated and partially characterized. Transient transfections with hGH reporter confirmed the promoter activity in Y1 cells. Putative elements for transcription factors involved in regulation were noted in the sequence. The promoter activity of some of the constructs is responsive to a treatment by forskolin, due to the presence of several CRE-like sequences.
Naville
D
D
INSERM-INRA U418, Hôpital Debrousse, Lyon, France.
Jaillard
C
C
Barjhoux
L
L
Durand
P
P
Bégeot
M
M
D000306:Adrenal Cortex Neoplasms; D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D017931:DNA Primers; D005091:Exons; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D018043:Receptors, Corticotropin / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D016385:TATA Box; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D016376:Oligonucleotides, Antisense; D018043:Receptors, Corticotropin