Isolation and analysis of a novel gene, HXC-26, adjacent to the rab GDP dissociation inhibitor gene located at human chromosome Xq28 region.
We screened potential promoter regions from NotI-linking cosmid clones mapped on human chromosome Xq28 region with our constructed trapping vector and isolated six fragments containing transcription activity. Using one of the obtained fragments as a probe, a novel gene was isolated by screening a human skeletal muscle cDNA library. The isolated cDNA, termed HXC-26, contained an open reading frame of 975 nucleotides encoding 325 amino acids (38,848 Da). The HXC-26 gene was composed of 13 exons that span approximately 8 kb. Several potential GC boxes were found in the putative promoter region, but no typical TATA box. The HXC-26 gene associated with a CpG island was located adjacent to the rab GDP dissociation inhibitor (GDI) gene.
Toyoda
A
A
Department of Bioengineering, Faculty of Engineering, Soka University, Tokyo, Japan. atoyoda@t.soka.ac.jp
Sakai
T
T
Sugiyama
Y
Y
Kusuda
J
J
Hashimoto
K
K
Maeda
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018899:CpG Islands; D019204:GTP-Binding Proteins / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D020730:Guanine Nucleotide Dissociation Inhibitors; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014960:X Chromosome / Q000235:genetics*; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
D020730:Guanine Nucleotide Dissociation Inhibitors; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D019204:GTP-Binding Proteins