Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene.
Yarrowia lipolytica SEC62 cDNA was cloned by functional complementation of a thermo-sensitive sec62 Saccharomyces cerevisiae mutant strain. The Y. lipolytica SEC62 promoter region was amplified by the inverse polymerase chain reaction (PCR). The cDNA codes for a 396 amino-acid protein with two potential trans-membrane domains. Y. lipolytica Sec62p behaves as an integral membrane protein as shown by Western blotting. Y. lipolytica SEC62 cDNA is able to complement a S. cerevisiae sec62 null mutant strain confirming functional conservation, although only 53.6% amino-acid similarity is observed between Y. lipolytica and S. cerevisiae Sec62.
Swennen
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Laboratoire de Génétique Moléculaire et Cellulaire, INRA, CNRS, INA Paris-Grignon, F-78850 Thiverval Grignon, France.
Joyet
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Gaillardin
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D000595:Amino Acid Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004271:DNA, Fungal / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000276:immunology; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C074213:SEC62 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins