title: Effect of TYB-2285 on passive cutaneous anaphylaxis in rats.
abstract: 1. TYB-2285 (1-30 mg/kg p.o.) inhibited ovalbumin (OA)- and dinitrophenyl-Ascaris (DNPAs)-induced passive cutaneous anaphylaxis (PCA) in a dose-dependent manner. 2. The ED50 of TYB-2285 and ketotifen fumarate on OA-induced PCA were 0.5 and 3.9 mg/kg, respectively. The ED50 of TYP-2285 and amlexanox on DNP-As-induced PCA were 3.5 and 0.9 mg/ kg, respectively. 3. TYB-2285 (3-30 mg/kg p.o.) inhibited histamine consumption at the PCA site. 4. Unlike cyproheptadine or amlexanox, TYB-2285 (30 mg/kg p.o.) did not inhibit histamine-, serotonin-, ascites-, 48/80-, or A23187-induced capillary permeability. It inhibited dextran-induced capillary permeability slightly. 5. These results demonstrate that TYB-2285 inhibits PCA by inhibiting histamine release, although it does not inhibit capillary permeability.
authors.affiliation: Department of Pharmacology, Toyobo Co. Ltd., Shiga, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Tominaga
authors.forename: H
authors.initials: H
authors.lastname: Shutoh
authors.forename: H
authors.initials: H
authors.lastname: Hayashi
authors.forename: J
authors.initials: J
authors.lastname: Tsuji
authors.forename: A
authors.initials: A
authors.lastname: Koda
authors.forename: H
authors.initials: H
authors.lastname: Nagai
authors.forename: Y
authors.initials: Y
authors.lastname: Kumazawa
authors.forename: H
authors.initials: H
authors.lastname: Shimada
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000947:Antigens, Helminth / Q000276:immunology; D017165:Ascaris suum / Q000276:immunology; D002199:Capillary Permeability / Q000187:drug effects; D004136:Dinitrobenzenes / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000378:metabolism; D008297:Male; D009570:Nitriles / Q000494:pharmacology*; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D013997:Time Factors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000947:Antigens, Helminth; D004136:Dinitrobenzenes; D009570:Nitriles; C081050:3,5-bis(acetoxyacetylamino)-4-chlorobenzonitrile; D006632:Histamine; D010047:Ovalbumin