The use of osazones as matrices for the matrix-assisted laser desorption/ionization mass spectrometry of carbohydrates.
Fifteen sugar osazones were synthesized and evaluated for their use as matrix-assisted laser desorption/ ionization matrices. Various carbohydrate samples were analyzed, indicating that D- or L-arabinosazone, prepared from D- or L-arabinose and phenylhydrazine, gave the overall best performance. The arabinosazones facilitated superior analyses in providing a highly uniform distribution of sample molecules within the matrix microcrystals, and a possibility of using low laser energy for irradiation, leading to high spectral resolution and low matrix background. The arabinosazones with a moderate content of sodium ions promoted specific fragmentation for certain carbohydrates tested. The 50 fmol amounts of maltohexaose and maltoheptaose (present in 0.1 microliter of sample/matrix solution) were detected at a signal-to-background ratio of 3:1.
Chen
P
P
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Baker
A G
AG
Novotny
M V
MV
D001089:Arabinose / Q000737:chemistry; D005841:Gentisates; D005936:Glucans / Q000032:analysis; D006835:Hydrazones / Q000737:chemistry*; D062385:Hydroxybenzoates; D009005:Monosaccharides / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis; D012680:Sensitivity and Specificity; D012964:Sodium / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D005841:Gentisates; D005936:Glucans; D006835:Hydrazones; D062385:Hydroxybenzoates; D009005:Monosaccharides; D009844:Oligosaccharides; C016549:maltohexaose; C031146:maltoheptaose; D012964:Sodium; D001089:Arabinose; C010925:2,5-dihydroxybenzoic acid