Protective immunity induced by a Yersinia enterocolitica serovar 0:8 cellular extract.
The purpose of this study was to investigate the protective power of a cellular extract (CE) from Y. enterocolitica 0:8 grown in condition of expression of chromosomal antigens. Mice were immunized by s.c. route and challenged with: 0 LD50 (1 x 10(4) CFU/ml). Immunoblotting showed that CE-specific serum reacted with several CE antigens. Prominent bands, of molecular weights 60 and 35.5, were present in cytoplasmic and membrane fraction, respectively. The lipopolysaccharide (LPS) was detected in CE. These findings suggest that chromosomally-encoded antigens present in CE may induce protection against Y. enterocolitica infection. Both humoral and cellular immune response contribute to protection in mice.
Di Genaro
M S
MS
Facultad de Química, Bioquimica y Farmacia, Universidad Nacional de San Luis, Argentina.
Escudero
M E
ME
Maccioni
M
M
Stefanini de Guzmán
A M
AM
D019264:Adoptive Transfer; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D002876:Chromosomes, Bacterial / Q000235:genetics; D005260:Female; D007111:Immunity, Cellular; D007114:Immunization; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D013047:Specific Pathogen-Free Organisms; D015009:Yersinia Infections / Q000276:immunology / Q000517:prevention & control; D015008:Yersinia enterocolitica / Q000145:classification / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides