Effect of 5,6,7,8-tetrahydroneopterin on oxidative modification of low-density lipoprotein, and its uptake in the macrophage-like cell line J774.
Since oxidative modification of low-density lipoprotein (LDL) and uptake of the oxidized LDL by macrophages are closely related to the pathogenesis of atherosclerosis, we examined the inhibitory effect of 5,6,7,8-tetrahydroneopterin (NPH4) on Cu(II)-induced lipid peroxidation of LDL. NPH4 significantly inhibited both lipid peroxidation and the associated increase of electrophoretic mobility of ApoB protein. Furthermore, NPH4 suppressed the uptake of oxidized [14C]oleyl-esterified LDL by the macrophage-like cell line J774. NPH4 may be a candidate for the treatment of atherosclerosis and other active oxygen-related diseases.
Iizuka
H
H
Research Institute for Biosciences, Science University of Tokyo, Chiba, Japan.
Watanabe
Y
Y
Kojima
S
S
D000975:Antioxidants / Q000494:pharmacology*; D001055:Apolipoproteins B / Q000737:chemistry; D001708:Biopterins / Q000031:analogs & derivatives* / Q000494:pharmacology; D001797:Blood Protein Electrophoresis; D002460:Cell Line; D003300:Copper / Q000494:pharmacology; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000378:metabolism
D000975:Antioxidants; D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D010713:Phosphatidylcholines; D001708:Biopterins; C023455:tetrahydroneopterin; D003300:Copper