title: Structure and expression of a cluster of glutathione S-transferase genes from a marine fish, the plaice (Pleuronectes platessa).
abstract: Glutathione S-transferases are involved in the detoxification of reactive electrophilic compounds, including intracellular metabolites, drugs, pollutants and pesticides. A cluster of three glutathione S-transferase genes, designated GSTA, GSTA1 and GSTA2, was isolated from the marine flatfish, plaice (Pleuronectes platessa). GSTA and GSTA1 code for protein products with 76% amino acid identity. GSTA2 appears to contain a single nucleotide deletion which would render any product non-functional. All of these genes consist of six exons of similar sizes and greater than 70% nucleotide identity, and are interrupted by five introns of differing sizes. GSTA and GSTA1 mRNAs were present in a range of tissues, while GSTA2 mRNA was no detected. Expression of GSTA mRNA was increased in plaice intestine and spleen by pretreatment with beta-naphthoflavone, and expression of both GSTA and GSTA1 mRNAs was increased in plaice liver and gill by pretreatment with the peroxisome proliferating agent perfluoro-octanoic acid.
authors.affiliation: NERC Unit of Aquatic Biochemistry, University of Stirling, Scotland, U.K.
authors.forename: M J
authors.initials: MJ
authors.lastname: Leaver
authors.forename: J
authors.initials: J
authors.lastname: Wright
authors.forename: S G
authors.initials: SG
authors.lastname: George
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005412:Flatfishes / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes; D005880:Gills / Q000201:enzymology; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D007422:Intestines / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000187:drug effects / Q000201:enzymology; D019324:beta-Naphthoflavone / Q000494:pharmacology
chemical_list: D004251:DNA Transposable Elements; D019324:beta-Naphthoflavone; D005982:Glutathione Transferase