title: Endothelial stunning and myocyte recovery after reperfusion of jeopardized muscle: a role of L-arginine blood cardioplegia.
abstract: Ischemia and reperfusion may damage myocytes and endothelium in jeopardized hearts. This study tested whether (1) endothelial dysfunction (reduced nitric oxide release) exists despite good contractile performance and (2) supplementation of blood cardioplegic solution with nitric oxide precursor L-arginine augments nitric oxide and restores endothelial function. Among 30 Yorkshire-Duroc pigs, 6 received standard glutamate/aspartate blood cardioplegic solution without global ischemia. Twenty-four underwent 20 minutes of 37 degrees C global ischemia. Six received normal blood reperfusion. In 18, the aortic clamp remained in place 30 more minutes and all received 3 infusions of blood cardioplegic solution. In 6, the blood cardioplegic solution was unaltered; in 6, the blood cardioplegic solution contained L-arginine (a nitric oxide precursor) at 2 mmol/L; in 6, the blood cardioplegic solution contained the nitric oxide synthase inhibitor L-nitro arginine methyl ester (L-NAME) at 1 mmol/L. Complete contractile and endothelial recovery occurred without ischemia. In jeopardized hearts, complete systolic recovery followed infusion of blood cardioplegic solution and of blood cardioplegic solution plus L-arginine. Conversely, contractility recovered approximately 40% after infusion of normal blood and blood cardioplegic solution plus L-NAME. Postischemic nitric oxide production fell 50% in the groups that received blood cardioplegic solution and blood cardioplegic solution plus L-NAME but was increased in the group that received blood cardioplegic solution L-arginine. In vivo endothelium-dependent vasodilator responses to acetylcholine recovered 75% +/- 5% of baseline in the blood cardioplegic solution plus L-arginine group, but less than 20% of baseline in other jeopardized hearts. Endothelium-independent smooth muscle responses to sodium nitroprusside were relatively unaltered. Myeloperoxidase activity (neutrophil accumulation) was similar in the blood cardioplegic solution (without ischemia) and blood cardioplegic solution plus L-arginine groups (0.01 +/- 0.002 vs 0.013 +/- 0.003 microgram/gm tissue). Myeloperoxidase activity was raised substantially to 0.033 +/- 0.002 microgram/gm after exposure to normal blood and to 0.025 +/- 0.003 microgram/gm after infusion of blood cardioplegic solution and was highest at 0.053 +/- 0.01 microgram/gm with exposure to blood cardioplegic solution plus L-NAME in jeopardized hearts. The discrepancy between contractile recovery and endothelial dysfunction in jeopardized muscle can be reversed by adding L-arginine to blood cardioplegic solution.
authors.affiliation: Division of Cardiothoracic Surgery, UCLA School of Medicine 90095-1741, USA.
authors.forename: A
authors.initials: A
authors.lastname: Mizuno
authors.forename: R
authors.initials: R
authors.lastname: Baretti
authors.forename: G D
authors.initials: GD
authors.lastname: Buckberg
authors.forename: H H
authors.initials: HH
authors.lastname: Young
authors.forename: J
authors.initials: J
authors.lastname: Vinten-Johansen
authors.forename: X L
authors.initials: XL
authors.lastname: Ma
authors.forename: L J
authors.initials: LJ
authors.lastname: Ignarro
mesh_terms: D000818:Animals; D002314:Cardioplegic Solutions; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000502:physiology; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D017682:Myocardial Stunning / Q000517:prevention & control*; D009206:Myocardium / Q000166:cytology* / Q000201:enzymology; D019331:NG-Nitroarginine Methyl Ester / Q000627:therapeutic use*; D009195:Peroxidase / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature; D014664:Vasodilation / Q000502:physiology
chemical_list: D002314:Cardioplegic Solutions; D004791:Enzyme Inhibitors; D009195:Peroxidase; D019331:NG-Nitroarginine Methyl Ester