title: Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80.
abstract: The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggest that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a BsoF I restriction site immediately adjacent to the repeat region.
authors.affiliation: Department of Biology, Florida International University, Miami 33199, USA. gdunca01@servax.fiu.edu
authors.forename: G T
authors.initials: GT
authors.lastname: Duncan
authors.forename: K
authors.initials: K
authors.lastname: Balamurugan
authors.forename: B
authors.initials: B
authors.lastname: Budowle
authors.forename: M L
authors.initials: ML
authors.lastname: Tracey
mesh_terms: D002878:Chromosomes, Human, Pair 1; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic
chemical_list: C069758:endodeoxyribonuclease Fnu4HI; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases