Effect of ethylene thiourea on cultured rat embryos in the presence of hepatic microsomal fraction.
To investigate whether teratogenicity of ethylene thiourea (ETU) is due to ETU per se or to its metabolites, rat embryos cultured in vitro were exposed to 10 and 30 micrograms/ml of ETU in the presence or absence of rat liver microsomal fraction (S9mix). In the absence of S9mix, the incidence of morphological abnormalities increased dose-dependently. In the presence of S9mix, abnormal morphogenesis was almost absent in all embryos. These findings suggest that teratogenicity of ETU is due to ETU per se.
Iwase
T
T
Department of Anatomy II, Azabu University, School of Veterinary Medicine, Kanagawa, Japan.
Yamamoto
M
M
Shirai
M
M
Akahori
F
F
Masaoka
T
T
Takizawa
T
T
Arishima
K
K
Eguchi
Y
Y
D000014:Abnormalities, Drug-Induced; D000818:Animals; D001711:Biotransformation; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D008862:Microsomes, Liver / Q000378:metabolism*; D009024:Morphogenesis / Q000187:drug effects; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013723:Teratogens / Q000493:pharmacokinetics / Q000633:toxicity*; D013890:Thiourea / Q000493:pharmacokinetics* / Q000633:toxicity*
D013723:Teratogens; D013890:Thiourea