Hemolysins and plasmid profiles of Vibrio parahaemolyticus.
Forty clinical isolates of Vibrio parahaemolyticus were studied for the production of the thermostable direct hemolysin (TDH), and the TDH-related hemolysin (TRH) including the respective encoding genes, tdh and trh. The presence of TDH and its encoding genes were found amongst 95% of the strains, whereas the TRH was absent amongst these isolates. Thirty-two isolates were found to be plasmid-free, whereas eight isolates possessed plasmids with sizes ranging from 2.4 > or = 23 kb. Using a DNA probe coding for the homologous region of the tdh and trh, it was found that the tdh genes were present on the chromosomal DNA.
Vadivelu
J
J
Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
Puthucheary
S D
SD
Mitin
A
A
Wan
C Y
CY
van Melle
B
B
Puthucheary
J A
JA
D001431:Bacteriological Techniques; D015342:DNA Probes; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000382:microbiology*; D005759:Gastroenteritis / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000097:blood*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D014735:Vibrio Infections / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000235:genetics*
D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D006460:Hemolysin Proteins