Free cytosolic calcium levels modify intracellular pH in rat pancreatic acini.
We have used BCECF- or Fura-2-loaded rat pancreatic acinar cells to investigate the relationship between Ca2+ mobilization and intracellular pH (pHi). Ca2+-mobilizing agonists CCK-8 and ACh induced a transient acidification totally dependent on release of Ca2+ from internal stores. Employment of different physiological tools including ionomycin and thapsigargin to increase the cytosolic Ca2+ concentration and capacitative calcium influx also induced cellular acidification. Application of 1mM LaCl3 reduced the CCK-8-evoked acidification. These data indicate that the mobilization of intracellular Ca2+ stores by CCK-8 decreases cellular pH by Ca2+/H+ exchanger.
González
A
A
Department of Physiology, University of Extremadura, Caceres, Spain.
Camello
P J
PJ
Pariente
J A
JA
Salido
G M
GM
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology; D002469:Cell Separation; D003600:Cytosol / Q000502:physiology; D005110:Extracellular Space / Q000187:drug effects / Q000502:physiology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007424:Intracellular Fluid / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012844:Sincalide / Q000494:pharmacology
D000252:Calcium-Transporting ATPases; D012844:Sincalide; D000109:Acetylcholine; D002118:Calcium