High efficiency transformation of Bacillus brevis by electroporation.
Various conditions in the Bacillus brevis transformation by electroporation were investigated to increase the efficiency. Competent cell volume and presence of polyethylene glycol 6000 and single stranded DNA on pulsing were critical for the efficiency. As a result, we established the improved method by which 10(7)-10(8) transformants/microgram plasmid DNA could be obtained.
Okamoto
A
A
Department of Brewing and Fermentation, Tokyo University of Agriculture, Japan.
Kosugi
A
A
Koizumi
Y
Y
Yanagida
F
F
Udaka
S
S
D001407:Bacillus / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D014169:Transformation, Bacterial
D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols