title: Postsynaptic element contributes to the delay in synaptogenesis in synapsin I-deficient neurons.
abstract: In a previous study, we reported a retardation in process outgrowth and synapse formation in cultured hippocampal neurons from synapsin I-deficient mice. Here we investigated whether this delay in synaptogenesis was attributable to pre- or postsynaptic elements. The experimental paradigm used in this study involved the establishment of heterochronic cocultures of neurons from wild-type and synapsin I-deficient neurons established synapses with mature wild-type postsynaptic elements after 24 and 72 h, respectively. In contrast, synapsin I-deficient postsynaptic elements were able to receive synapses only after 9 days in culture, representing a 5-day delay compared to controls. The results suggest a broad role of synapsin I in the structural development of the synapse, participating directly or indirectly in the maturation of both presynaptic and postsynaptic sites.
authors.affiliation: Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
authors.forename: A
authors.initials: A
authors.lastname: Ferreira
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: L S
authors.initials: LS
authors.lastname: Chin
authors.forename: P
authors.initials: P
authors.lastname: Greengard
authors.forename: K S
authors.initials: KS
authors.lastname: Kosik
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002452:Cell Count; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology / Q000648:ultrastructure; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000648:ultrastructure; D051379:Mice / Q000196:embryology; D009474:Neurons / Q000378:metabolism* / Q000502:physiology* / Q000648:ultrastructure; D013569:Synapses / Q000502:physiology*; D016704:Synapsins / Q000172:deficiency*; D013997:Time Factors
chemical_list: D016704:Synapsins