Determination of vinca alkaloids in human plasma by liquid chromatography/atmospheric pressure chemical ionization mass spectrometry.
A sensitive assay was developed for the quantitation of vinblastine, desacetylvinblastine and vincristine using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS). Analyses were performed on an Ultrasphere C18 microbore column using ammonium acetate as mobile phase. The calibration curves were linear across the range of 0.51-4.00 ng/ml (0.63-4.93 nM) for vinblastine, 0.74-3.93 ng/ml (0.96-5.11 nM) for desacetylvinblastine and 0.30-3.95 ng/ml (0.36-4.79 nM) for vincristine. Vinca alkaloid concentrations were measured with an accuracy and precision within 11%. This assay could be implemented to determine the plasma concentrations for pharmacokinetic studies of vinblastine, desacetylvinblastine and vincristine in conjunction with clinical trials.
Ramírez
J
J
Department of Medicine, University of Chicago Medical Center, IL 60637, USA.
Ogan
K
K
Ratain
M J
MJ
D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry; D014747:Vinblastine / Q000097:blood; D014748:Vinca Alkaloids / Q000097:blood*; D014750:Vincristine / Q000097:blood
D014748:Vinca Alkaloids; D014750:Vincristine; D014747:Vinblastine