title: Instability, unfolding and aggregation of human lysozyme variants underlying amyloid fibrillogenesis.
abstract: Tissue deposition of soluble proteins as amyloid fibrils underlies a range of fatal diseases. The two naturally occurring human lysozyme variants are both amyloidogenic, and are shown here to be unstable. They aggregate to form amyloid fibrils with transformation of the mainly helical native fold, observed in crystal structures, to the amyloid fibril cross-beta fold. Biophysical studies suggest that partly folded intermediates are involved in fibrillogenesis, and this may be relevant to amyloidosis generally.
authors.affiliation: Immunological Medicine Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
authors.forename: D R
authors.initials: DR
authors.lastname: Booth
authors.forename: M
authors.initials: M
authors.lastname: Sunde
authors.forename: V
authors.initials: V
authors.lastname: Bellotti
authors.forename: C V
authors.initials: CV
authors.lastname: Robinson
authors.forename: W L
authors.initials: WL
authors.lastname: Hutchinson
authors.forename: P E
authors.initials: PE
authors.lastname: Fraser
authors.forename: P N
authors.initials: PN
authors.lastname: Hawkins
authors.forename: C M
authors.initials: CM
authors.lastname: Dobson
authors.forename: S E
authors.initials: SE
authors.lastname: Radford
authors.forename: C C
authors.initials: CC
authors.lastname: Blake
authors.forename: M B
authors.initials: MB
authors.lastname: Pepys
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D000686:Amyloidosis / Q000201:enzymology / Q000235:genetics*; D002942:Circular Dichroism; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D006358:Hot Temperature; D006801:Humans; D008107:Liver Diseases / Q000235:genetics / Q000473:pathology; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D017354:Point Mutation; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000682:Amyloid; D011994:Recombinant Proteins; D009113:Muramidase