The cloning and characterization of human MyD88: a member of an IL-1 receptor related family.
Murine MyD88, an RNA with homology both to the interleukin-1 receptor signaling domain and to 'death-domains', is rapidly upregulated during differentiation of the myeloleukemic cell line M1. We have cloned the human homologue of murine MyD88 and re-evaluated the murine sequence. The open reading frame for both species encodes a 296 amino acid protein, which for murine MyD88 is 53 amino acids longer than originally published. Human MyD88 cDNA is encoded by 5 exons, and maps to chromosome 3p21.3-p22 by fluorescence in situ hybridization (FISH). Overexpression of the death domain region leads to transcriptional activation of the IL-8 promoter.
Bonnert
T P
TP
Immunex Corporation, Seattle, WA 98101, USA. tim_bonnert@merck.com
Garka
K E
KE
Parnet
P
P
Sonoda
G
G
Testa
J R
JR
Sims
J E
JE
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D000943:Antigens, Differentiation; D001483:Base Sequence; D019556:COS Cells; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016209:Interleukin-8 / Q000235:genetics; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88; D016376:Oligonucleotides, Antisense; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1 / Q000737:chemistry / Q000235:genetics; D014162:Transfection
D048868:Adaptor Proteins, Signal Transducing; D000943:Antigens, Differentiation; D018076:DNA, Complementary; D016209:Interleukin-8; C507779:MYD88 protein, human; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D016376:Oligonucleotides, Antisense; D011506:Proteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1