Cholinergic stimulation of human microcytoma cell line H69.
Experiments were addressed to investigate the mechanisms by which cholinergic stimulation is coupled to the enhancement of proliferation of small cell lung cancer cells H69. Muscarinic stimulation triggers the release of cytosolic Ca2+ and of inositol(1,4,5)trisphosphate with comparable time courses. The presence of alpha-bungarotoxin or the absence of Ca2+ in external medium suppresses enhancement of clonal growth induced by brief applications of nicotine. Here we suggest that Ca2+ mobilization represents a trigger for the enhancement of small cell lung cancer cell proliferation upon cholinergic stimulation.
Fucile
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S
Laboratorio di Biofisica, Centro Ricerca Sperimentale Istituto Regina Elena, Rome, Italy.
Napolitano
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M
Mattei
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E
D000109:Acetylcholine / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D018288:Carcinoma, Small Cell / Q000378:metabolism* / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D004594:Electrophysiology; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D009116:Muscarine / Q000494:pharmacology; D018002:Receptors, Bradykinin / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D014407:Tumor Cells, Cultured
D018002:Receptors, Bradykinin; D009116:Muscarine; D000109:Acetylcholine; D002118:Calcium