High-performance chromatographic assay for the sulphoxide metabolite of 2'-deoxy-3'-thiacytidine in human urine.
A high-performance liquid chromatographic method is described for the determination in human urine of GI138870X, the sulphoxide metabolite of a novel dideoxynucleoside analogue, 2'-deoxy-3'-thiacytidine (lamivudine). GI138870X was extracted from human urine using Empore SDB RPS solid-phase extraction disks prior to reversed-phase chromatography with UV detection. The method has shown to be valid over the concentration range 0.5-100 micrograms/ml using a 0.5-ml sample volume.
Plumb
R S
RS
Bioanalysis and Drug Metabolism Department, GlaxoWellcome Research and Develpment, Greenford, Middleses, UK.
Gray
R D
RD
Harker
A J
AJ
Taylor
S
S
D019380:Anti-HIV Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003499:Cyclic S-Oxides / Q000652:urine*; D006801:Humans; D019259:Lamivudine / Q000031:analogs & derivatives* / Q000652:urine
D019380:Anti-HIV Agents; D003499:Cyclic S-Oxides; D019259:Lamivudine; C104840:lamivudine sulfoxide