title: Synthesis of secretory protein in regenerating liver of rat after partial hepatectomy.
abstract: Albumin immunoreactivity in the liver was examined on days 2, 5 and 10 after two-thirds partial hepatectomy by light and ultrastructural immunoperoxidase methods and the ultrastructural area of the rough endoplasmic reticulum (ER) in hepatocytes was measured. Albumin immunoreactivity was seen in the rough ER and Golgi apparatus of all hepatocytes in the hepatectomized liver and ultrastructural analysis showed a significantly greater area of rough ER on day 5 than on days 2 or 10. Albumin mRNA was studied by the in situ hybridization technique using radioisotopes and their numbers were determined visually. Albumin mRNA was present as grains in all hepatocytes and the grains varied in number during regeneration of the liver, being more abundant on day 5 than on days 2 or 10. The activity of [3H]-leucine incorporated into albumin synthesis, an indicator of translational activity, was higher on days 5 and 10 than on day 2 and was highest on day 5. In conclusion, albumin synthesis varied during liver regeneration after partial hepatectomy, being reduced at the peak of cell proliferation on day 2 and being most active on day 5.
authors.affiliation: Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Iwai
authors.forename: M
authors.initials: M
authors.lastname: Kashiwadani
authors.forename: Y
authors.initials: Y
authors.lastname: Harada
authors.forename: K
authors.initials: K
authors.lastname: Tada
authors.forename: Y
authors.initials: Y
authors.lastname: Ishii
authors.forename: Y
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: T
authors.initials: T
authors.lastname: Nakashima
authors.forename: T
authors.initials: T
authors.lastname: Okanoue
authors.forename: K
authors.initials: K
authors.lastname: Kashima
authors.forename: Y
authors.initials: Y
authors.lastname: Ibata
mesh_terms: D000418:Albumins / Q000096:biosynthesis*; D000818:Animals; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D006498:Hepatectomy; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000418:Albumins; D007930:Leucine