Transferable hyperproduction of TEM-1 beta-lactamase in Shigella flexneri due to a point mutation in the pribnow box.
TEM-1 hyperproduction in two ampicillin-sulbactam-resistant Shigella flexneri strains was studied. In both strains the blaTEM gene was encoded as a single copy on a large conjugatively transferable plasmid. A single G-->T transversion at position 1 of the -10 consensus sequence was identified to be the mechanism of TEM-1 hyperproduction.
Siu
L K
LK
Department of Microbiology, Queen Mary Hospital Compound, University of Hong Kong, Hong Kong.
Ho
P L
PL
Yuen
K Y
KY
Wong
S S
SS
Chau
P Y
PY
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D015169:Colony Count, Microbial; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012763:Shigella flexneri / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1