title: Antioxidant and prooxidant role of beta-carotene in murine normal and tumor thymocytes: effects of oxygen partial pressure.
abstract: The effects of the partial pressure of oxygen (pO2) on antioxidant efficiency of beta-carotene in inhibiting radical-initiated lipid peroxidation were studied in murine normal and tumor thymocytes. At 150 mm Hg pO2 (the pressure of oxygen in normal air), beta-carotene acted as an antioxidant, inhibiting radical-induced lipid peroxidation in both normal and tumor thymocytes. At 760 mm Hg p02, beta-carotene lost its antioxidant activity in normal thymocytes and exhibited a dose-dependent prooxidant effect in tumor thymocytes. In these cells, the prooxidant effect of beta-carotene was also accompanied by an increase of endogenous alpha-tocopherol loss. beta-Carotene radical-trapping and autooxidation reactions were faster at 760 mm Hg pO2 than at 150 mm Hg pO2 in both normal and tumor thymocytes and the carotenoid was more rapidly consumed in tumor cells. These data point out a key role of the oxygen tension on the antioxidant effectiveness of beta-carotene. They also show a selective prooxidant effect of beta-carotene under 100% oxygen in tumor cells.
authors.affiliation: Institute of General Pathology, Catholic University, Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Palozza
authors.forename: C
authors.initials: C
authors.lastname: Luberto
authors.forename: G
authors.initials: G
authors.lastname: Calviello
authors.forename: P
authors.initials: P
authors.lastname: Ricci
authors.forename: G M
authors.initials: GM
authors.lastname: Bartoli
mesh_terms: D000578:Amidines / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants; D005609:Free Radicals; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016877:Oxidants; D010100:Oxygen / Q000008:administration & dosage*; D010313:Partial Pressure; D013950:Thymus Gland / Q000187:drug effects* / Q000378:metabolism; D013953:Thymus Neoplasms / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism; D019207:beta Carotene / Q000494:pharmacology*
chemical_list: D000578:Amidines; D000975:Antioxidants; D005609:Free Radicals; D016877:Oxidants; D014970:Xanthines; D019207:beta Carotene; D014810:Vitamin E; D019820:Xanthine; C046728:2,2'-azobis(2-amidinopropane); D014969:Xanthine Oxidase; D010100:Oxygen