Lymph node localisation of biodegradable nanospheres surface modified with poloxamer and poloxamine block co-polymers.
Studies were performed to develop a sub-100 nm biodegradable colloidal system for the efficient delivery of drugs and diagnostic agents to the lymphatic system. Nanospheres of poly(lactide-co-glycolide) were prepared by interfacial polymer deposition. The nanospheres were coated with block co-polymers in order to modify their surface characteristics. Radiolabelling of the nanospheres for in vivo tracing was achieved by the incorporation of the lipophilic complex 111In-oxine during nanosphere preparation. In vitro stability of the radiolabelled nanospheres was determined in rat serum at 37 degrees C. The lymphatic distribution of the nanospheres was determined after subcutaneous administration to the rat. Lymphatic uptake of all coated systems was enhanced compared to the uncoated nanospheres, and a maximal uptake of 17% of the administered dose in the regional lymph nodes was achieved. These observations suggest that the nanospheres are suitable for diagnostic and therapeutic applications in clinical and experimental medicine.
Hawley
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AE
Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
Illum
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Davis
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D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D004337:Drug Carriers; D005029:Ethylenediamines; D019344:Lactic Acid; D008198:Lymph Nodes / Q000378:metabolism*; D008297:Male; D008863:Microspheres; D010316:Particle Size; D011060:Poloxalene; D011092:Polyethylene Glycols; D011098:Polyglactin 910 / Q000493:pharmacokinetics*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties
D001672:Biocompatible Materials; D004337:Drug Carriers; D005029:Ethylenediamines; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011098:Polyglactin 910; D011092:Polyethylene Glycols; D011060:Poloxalene