Micropreparation of hemopoietic stem cells from the mouse bone marrow suspension by gravitational field-flow fractionation.
Gravitational field-flow fractionation is a relatively simple experimental technique. This method was used for the characterization of stem cells from mouse bone marrow. Because these cells are bigger than the other cells in bone marrow, it is possible to separate them from the mixture. The fractions collected after passing through the separation channel were characterized using a Coulter Counter and used for transplantation into irradiated mice.
Urbánková
E
E
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
Vacek
A
A
Chmelík
J
J
D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology