Biochemical alterations in paromomycin-treated Leishmania donovani promastigotes.
Paromomycin is used for the treatment of leishmaniasis in humans, but little is known about its mechanism of action. Investigating the effect of this antibiotic on promastigotes of Leishmania donovani, we showed that inhibition of the multiplication of these parasites could be related to its effect on RNA synthesis and to modifications of membranous polar lipids and membrane fluidity, leading to altered membrane permeability.
Maarouf
M
M
Institut de Chimie des Substances Naturelles, C.N.R.S., Gif-sur-Yvette, France.
Lawrence
F
F
Brown
S
S
Robert-Gero
M
M
D000563:Amebicides / Q000494:pharmacology; D000818:Animals; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D016054:DNA, Protozoan / Q000096:biosynthesis; D006801:Humans; D007893:Leishmania donovani / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D009355:Neomycin / Q000494:pharmacology; D010303:Paromomycin / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000096:biosynthesis; D016053:RNA, Protozoan / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014529:Uridine / Q000378:metabolism
D000563:Amebicides; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D010303:Paromomycin; D002701:Chloramphenicol; D003513:Cycloheximide; D007930:Leucine; D009355:Neomycin; D013936:Thymidine; D014529:Uridine