Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines.
In recent years there has been considerable progress towards the development of expression systems for the display of heterologous polypeptides and, to a lesser extent, oligosaccharides on the surface of bacteria or yeast. The availability of protein display vectors has in turn provided the impetus for a range of exciting technologies. Polypeptide libraries can be displayed in bacteria and screened by cell sorting techniques, thus simplifying the isolation of proteins with high affinity for ligands. Expression of antigens on the surface of nonvirulent microorganisms is an attractive approach to the development of high-efficacy recombinant live vaccines. Finally, cells displaying protein receptors or antibodies are of use for analytical applications and bioseparations.
Georgiou
G
G
Department of Chemical Engineering, University of Texas, Austin. gg@che.utexas.edu
Stathopoulos
C
C
Daugherty
P S
PS
Nayak
A R
AR
Iverson
B L
BL
Curtiss
R
R
D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D005544:Forecasting; D015723:Gene Library; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D013210:Staphylococcus / Q000737:chemistry / Q000235:genetics; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; D010455:Peptides; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic