[Pillin structural gene and bacterial adhesion to cultured cell of Pseudomonas aeruginosa isolated from clinical materials].
Thirty-three stocks of Pseudomonas aeruginosa which is an important etiologic agent of opportunistic infections were clinically isolated. The pillin structural gene pilA of the stocks were amplified by polymerase chain reaction (PCR) and classified into 3 groups; 2000 bp (16 stocks; 48.5%), 1300 bp (11 stocks; 33.3%), 550 bp (6 stocks; 18.2%). The adhesiveness of the stocks to cultured human lung cancer origin calu-1 was also determined, their adhesion rate per cell were 39.2%, 24.8%, 22.1% in average respectively. Thus clinically most common 2000 pb group is remarkably easier to adhere to calu-1. Serotypes of the strains were examined to reveal the difference of the distribution that F. G. I types were dominant in 2000 pb group, but E types were major in 1300 bp and 550 bp groups. These data suggest that the gene arrangement of pilA influences adhesiveness to cultured cell and antigenicity of bacteria.
Nakazawa
Y
Y
Internal Medicine, Jikei University School of Medicine.
D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002478:Cells, Cultured; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000502:physiology*
D001425:Bacterial Outer Membrane Proteins; D036781:Fimbriae Proteins