Adhesion properties of human alveolar macrophages with respect to extracellular matrix components and chemotactic agonists.
Adherence has an essential impact on the differentiation and activation of tissue dwelling monocytes/macrophages. We have considered the effect of selected chemotactic agonists (fMLP, RANTES, IL-8) on the adhesion properties of human alveolar macrophages prepared by bronchoalveolar lavage. The macrophages were co-incubated in buffer alone or buffer supplemented with respective agonist, for different time points, in culture wells precoated with albumin, vitronectin and fibronectin, respectively. The macrophages displayed a gradual increase in adhesion in all three surfaces and discriminated between the different matrix components, but did not respond to the selected agonists with increased adhesion.
Lundahl
J
J
Department of Clinical Immunology and Transfusion Medicine, Karolinska Hospital, Stockholm, Sweden.
Eklund
A
A
Halldén
G
G
Sköld
C M
CM
D000328:Adult; D000418:Albumins / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002448:Cell Adhesion / Q000187:drug effects*; D018946:Chemokine CCL5 / Q000494:pharmacology*; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D008262:Macrophage Activation; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D011485:Protein Binding; D013997:Time Factors; D019096:Vitronectin / Q000378:metabolism
D000418:Albumins; D018946:Chemokine CCL5; D005353:Fibronectins; D016209:Interleukin-8; D019096:Vitronectin; D009240:N-Formylmethionine Leucyl-Phenylalanine