The MIC1 microneme protein of Toxoplasma gondii contains a duplicated receptor-like domain and binds to host cell surface.
The cDNA encoding the Toxoplasma gondii microneme protein MIC1 and the corresponding gene have been cloned and sequenced. The MIC1 gene contains three introns. The cDNA encodes a 456 amino acid (aa) sequence, with a typical signal sequence and no other trans-membrane domain. The protein contains a tandemly duplicated domain with conservation of cysteines and presents distant homology with the Plasmodium sp. microneme protein TRAP-SSP2. The MIC1 protein from tachyzoite lysates and a PMAL recombinant expressing the N-terminal duplicated domain of the protein bound to the surface of putative host cells, suggesting a possible involvement of MIC1 in host cell binding/recognition.
Fourmaux
M N
MN
INSERM U42, Villeneuve d' Ascq, France.
Achbarou
A
A
Mercereau-Puijalon
O
O
Biderre
C
C
Briche
I
I
Loyens
A
A
Odberg-Ferragut
C
C
Camus
D
D
Dubremetz
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014122:Toxoplasma / Q000235:genetics* / Q000378:metabolism; D014709:Vero Cells
D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; C105877:MIC1 protein, Toxoplasma gondii; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins