title: Optimised method for determination of ticlopidine in serum by high performance liquid chromatography.
abstract: In this study we verified whether high performance liquid chromatography with UV detection (HPLC-UV) could be optimised and hence become a useful method for measuring ticlopidine concentrations in patient blood. The extraction step was improved by adding isoamylic alcohol in the extraction mixture, resulting in a better recovery. Moreover, the extraction efficiency was consistently ameliorated by evaporation to dryness of the organic extract; we could therefore utilise a lower (213 nm) wavelength, corresponding to the maximum of absorption, achieving a better sensitivity. With these technical improvements, the limit of quantitation is 0.02 microgram/ml, and the limit of detection is 0.01 microgram/ml, hence comparable to levels obtained with gas-liquid chromatography with nitrogen detection (GC-NPD). In addition, HPLC method, if compared with GC-NPD, is simpler and can be easily used for routine determinations of a lot of serum samples.
authors.affiliation: Laboratorio di Tossicologia, Istituto di Medicina Legale e delle Assicurazioni, Ospedale Maggiore, Trieste.
authors.forename: G
authors.initials: G
authors.lastname: Furlan
authors.forename: N
authors.initials: N
authors.lastname: Malusà
authors.forename: A
authors.initials: A
authors.lastname: Strohmayer
authors.forename: F B
authors.initials: FB
authors.lastname: Klugmann
authors.forename: G
authors.initials: G
authors.lastname: Decorti
authors.forename: L
authors.initials: L
authors.lastname: Candussio
authors.forename: S
authors.initials: S
authors.lastname: Klugmann
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010975:Platelet Aggregation Inhibitors / Q000097:blood*; D015203:Reproducibility of Results; D013988:Ticlopidine / Q000097:blood*
chemical_list: D010975:Platelet Aggregation Inhibitors; D013988:Ticlopidine