Antiproliferative potency of structurally distinct dietary flavonoids on human colon cancer cells.
Dietary flavonoids are known to be antiproliferative and may play an important role in cancer chemoprevention, especially cancers of the gastrointestinal tract, because of a direct contact with food. This study was designed to compare the antiproliferative potency of several structurally distinct dietary flavonoids in colon cancer cells, Caco-2 and HT-29, and in rat non-transformed intestinal crypt cells, IEC-6. Flavonoids varied significantly in their antiproliferative potency depending on the structural features but the observations were consistent among the three cell lines studied. Of the two most potent flavonoids, quercetin and genistein, the effect was found to be dose-dependent and chromatin condensation, an indication of apoptosis, was noticed. Quercetin was found to distribute throughout the cell with higher amounts in the perinuclear and nucleoli areas. The lack of specific cell membrane enrichment by quercetin was consistent with its lack of effect on the transepithelial resistance. While several flavonoids including quercetin were found to be unstable, the chemical instability did not correlate with the antiproliferative potency, although it may contribute to the antiproliferative effect.
Kuo
S M
SM
Nutrition Program, State University of New York at Buffalo, 14214, USA. smkuo@acsu.buffalo.edu
D000818:Animals; D017209:Apoptosis; D018938:Caco-2 Cells / Q000187:drug effects* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects / Q000473:pathology; D004355:Drug Stability; D005419:Flavonoids / Q000378:metabolism / Q000494:pharmacology*; D019833:Genistein; D019073:HT29 Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007529:Isoflavones / Q000737:chemistry; D008051:Lipid Bilayers / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011794:Quercetin / Q000737:chemistry; D051381:Rats
D005419:Flavonoids; D007529:Isoflavones; D008051:Lipid Bilayers; D011794:Quercetin; D019833:Genistein