Induction of apoptosis by ionizing radiation in Chinese hamster V79 cells and a radioresistant cell strain derived from V79.
DNA fragmentation into nucleosome ladder, a hall mark of apoptosis, could be obtained by as low as 0.58 Gy of gamma irradiation within 6 hr of irradiation which increased appreciably after 48 hr in V79 cells. In the same condition condensation of the nucleus and marginalization of the cytoplasm the characteristic morphology of apoptotic death were observed. Unirradiated controls had approximately 2% apoptotic cells. When cells were irradiated with 0.58 Gy, approximately 10% of the cells had the apoptotic morphology. This number increased to approximately 29% at 3.5 Gy dose. At a higher dose, apoptotic and necrotic cells were visualized. In radio resistant cells higher doses were required to induce morphological changes. The results indicated that gamma irradiation can induce apoptosis in Chinese hamster V79, fibroblast cell line and the radioresistant cell strain derived from V79 cells is also resistant to induction of apoptosis.
Ghosh
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R
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Bidhan Nagar, Calcutta, India.
Sengupta
S
S
Bhattacharyya
N P
NP
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004307:Dose-Response Relationship, Radiation; D011836:Radiation Tolerance