Detection and characterisation of anti-endomysial antibody in coeliac disease using human umbilical cord.
OBJECTIVE
To verify the effectiveness of human umbilical cord (HUC) in the detection of anti-endomysial antibodies (AEA) in coeliac disease and to characterize further these antibodies by studying tissue adsorption characteristics and antibody inhibition studies.


METHODS
AEA were detected on HUC and primate oesophagus in a blind study, using sera from 46 patients with untreated coeliac disease and 108 controls. Tissue adsorption studies were performed using homogenized tissue from rodent liver, HUC, primate oesophagus and human liver. Sera were adsorbed with each of these homogenates and antibody was detected using HUC, primate oesophagus and rat kidney. In the inhibition experiments AEA was detected on HUC, and inhibition of binding was attempted by pre-incubating the sections with antibodies against collagen types I, III and IV.


RESULTS
The sensitivity of AEA was 91% when detected on HUC, 89% when detected on primate oesophagus (93% and 91%, respectively, after exclusion of 1 patient with IgA deficiency). Specificity was 100% for both assays. Tissue adsorption studies showed identical results for AEA detected on both HUC or primate oesophagus, whereas antireticulin antibody was adsorbed only by rodent tissue. Blocking of the HUC with anticollagen antibodies did not prevent binding of AEA.


CONCLUSIONS
HUC is an effective substrate for the detection of AEA and may be superior to primate oesophagus. The antibody detected by HUC shows identical tissue adsorption specificities to that detected on primate oesophagus.
Yiannakou
J Y
JY
Department of Gastroenterology, St. Thomas Hospital, London, UK.
Dell'Olio
D
D
Saaka
M
M
Ellis
H J
HJ
Rosen-Bronson
S
S
Dumonde
D C
DC
Ciclitira
P J
PJ
D000293:Adolescent; D000328:Adult; D000818:Animals; D001323:Autoantibodies / Q000302:isolation & purification*; D002446:Celiac Disease / Q000175:diagnosis / Q000276:immunology*; D002648:Child; D003094:Collagen / Q000276:immunology; D004947:Esophagus / Q000276:immunology; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000706:statistics & numerical data; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000276:immunology*; D051381:Rats; D012452:Saguinus; D012680:Sensitivity and Specificity; D014470:Umbilical Cord / Q000276:immunology*
D001323:Autoantibodies; D003094:Collagen