A comparison of the inhibitory effect of cromoline and nedocromil Na on histamine release from airway metachromatic cells and from peripheral basophils.
Metachromatic cells are increased in the airways of asthmatic patients. We obtained metachromatic cells from asthmatic airways using an induced sputum technique. The histamine release following ConA, anti-IgE and anti-IgE + IL3 from those cells were evaluated before and following the addition of cromoline Na and nedocromil Na. Metachromatic cells had a higher rate of spontaneous histamine release when compared to peripheral basophils. Cromoline Na and nedocromil Na inhibited histamine release from triggered metachromatic cells but not from peripheral basophils. It is concluded that airway metachromatic cells from asthmatics behave differently than peripheral basophils.
Kivity
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Institute of Pulmonary and Allergic Diseases, Tel-Aviv Sourasky Medical Center, Israel.
On
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A
Agami
O
O
Levo
Y
Y
Fireman
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E
D000328:Adult; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D001249:Asthma / Q000097:blood / Q000276:immunology*; D001491:Basophils / Q000187:drug effects*; D001980:Bronchi / Q000166:cytology; D003208:Concanavalin A / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology*; D005260:Female; D005541:Forced Expiratory Volume; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007377:Interleukin-3 / Q000494:pharmacology; D008297:Male; D017835:Nedocromil / Q000494:pharmacology*; D013183:Sputum / Q000166:cytology
D018927:Anti-Asthmatic Agents; D000888:Antibodies, Anti-Idiotypic; D007377:Interleukin-3; D017835:Nedocromil; D003208:Concanavalin A; D007073:Immunoglobulin E; D004205:Cromolyn Sodium