title: Immune response to vaccination with DNA encoding the bovine viral diarrhea virus major glycoprotein gp53 (E2).
abstract: Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle which has not been controlled by classical vaccination. The region encoding the BVDV major glycoprotein gp53 (E2) known to possess virus-neutralizing activity was cloned into a mammalian expression vector under the human cytomegalovirus (CMV) intermediate early promoter. Intramuscular and intradermal administration of the recombinant plasmid DNA into BALB/c mice induced BVDV gp53-specific antibody responses to both biotypes (cytopathic and noncytopathic) of BVDV genotype 1, and to cytopathic BVDV genotype 2. BVDV-neutralizing antibodies were generated against BVDV genotype 1 strains and they also persisted 6 months after the last injection.
authors.affiliation: Faculté de médecine vétérinaire, Université de Montréal, Départment de pathologie et microbiologie, St-Hyacinthe, Que, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Harpin
authors.forename: B
authors.initials: B
authors.lastname: Talbot
authors.forename: M
authors.initials: M
authors.lastname: Mbikay
authors.forename: Y
authors.initials: Y
authors.lastname: Elazhary
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology / Q000517:prevention & control*; D019556:COS Cells / Q000382:microbiology; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004279:DNA, Viral / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008845:Microinjections; D009500:Neutralization Tests; D010957:Plasmids; D014162:Transfection; D014611:Vaccination; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D018076:DNA, Complementary; D004279:DNA, Viral; D014759:Viral Envelope Proteins; C076551:gp53, bovine viral diarrhea virus