Chiral separation of chloroquine using heparin as a chiral selector in high-performance liquid chromatography.
The chiral selectivity of a commercially available heparin affinity column was investigated for chloroquine. The (+) enantiomer eluted first, which is consistent with results reported previously using heparin as a chiral additive in capillary zone electrophoresis. The effects of pH, ionic strength, and organic modifier on the enantiodiscrimination were explored. A combination of electrostatic and hydrophobic interactions seems to play an important role in the enantiodiscrimination exhibited by this novel phase.
Staicup
A M
AM
Department of Chemistry, University of Hawaii-Manoa, Honolulu 96822, USA.
Gahm
K H
KH
Baldueza
M
M
D002738:Chloroquine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006493:Heparin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D002738:Chloroquine; D006493:Heparin