title: Further evidence for a common mechanism for shedding of cell surface proteins.
abstract: Pro-TNF alpha, Steel factor, type II IL-1R and IL-2R alpha were expressed in COS-7 cells and the generation of their soluble forms was examined. The release of all four proteins was strongly stimulated by the phorbol ester PMA and completely blocked by a hydroxamate-based inhibitor of metalloproteases. COS-7 cell membranes were found to cleave various synthetic pro-TNF alpha peptides with the same specificity as a partially purified TNF alpha converting enzyme purified from human monocytic cells, suggesting that the same enzyme may be responsible for at least some of the COS-7 cell shedding activity.
authors.affiliation: Immunex Corporation, Seattle, WA 98101, USA. mullbergj@immunex.com
authors.forename: J
authors.initials: J
authors.lastname: Müllberg
authors.forename: C T
authors.initials: CT
authors.lastname: Rauch
authors.forename: M F
authors.initials: MF
authors.lastname: Wolfson
authors.forename: B
authors.initials: B
authors.lastname: Castner
authors.forename: J N
authors.initials: JN
authors.lastname: Fitzner
authors.forename: C
authors.initials: C
authors.lastname: Otten-Evans
authors.forename: K M
authors.initials: KM
authors.lastname: Mohler
authors.forename: D
authors.initials: D
authors.lastname: Cosman
authors.forename: R A
authors.initials: RA
authors.lastname: Black
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D004151:Dipeptides / Q000494:pharmacology; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000378:metabolism; D019089:Stem Cell Factor / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004151:Dipeptides; D006877:Hydroxamic Acids; D008565:Membrane Proteins; C092152:N-((2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl)-3-(2'-naphthyl)alanylalanine, 2-aminoethylamide; D018123:Receptors, Interleukin; D019089:Stem Cell Factor; D014409:Tumor Necrosis Factor-alpha