Presence of paf-acether in human blood after thin-layer chromatography, but not after high-performance liquid chromatography purification.
After HPLC purification of human blood extracts, paf-acether (paf) was found exclusively as a lipoprotein-bound compound (lipopaf), whereas free-paf was absent. When the same samples (or lipopaf recovered from HPLC) were purified by TLC, both free-paf and lipopaf were detected. The free-paf detected in blood samples could thus result from lipopaf dissociation during TLC purification.
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INSERM U 200, Clamart, France.
Benveniste
J
J
D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006801:Humans; D008074:Lipoproteins / Q000032:analysis / Q000378:metabolism; D010972:Platelet Activating Factor / Q000032:analysis* / Q000378:metabolism
D008074:Lipoproteins; D010972:Platelet Activating Factor