Characterization of the 6'-N-aminoglycoside acetyltransferase gene aac(6')-Im [corrected] associated with a sulI-type integron.
The amikacin resistance gene aac(6')-Im [corrected] from Citrobacter freundii Cf155 encoding an aminoglycoside 6'-N-acetyltransferase was characterized. The gene was identified as a coding sequence of 521 bp located down-stream from the 5' conserved segment of an integron. The sequence of this aac(6')-Im [corrected] gene corresponded to a protein of 173 amino acids which possessed 64.2% identity in a 165-amino-acid overlap with the aac(6')-Ia gene product (F.C. Tenover, D. Filpula, K.L. Phillips, and J. J. Plorde, J. Bacteriol. 170:471-473, 1988). By using PCR, the aac(6')-Im [corrected] gene could be detected in 8 of 86 gram-negative clinical isolates from two Belgian hospitals, including isolates of Citrobacter, Klebsiella spp., and Escherichia coli. PCR mapping of the aac(6')-Im [corrected] gene environment in these isolates indicated that the gene was located within a sulI-type integron; the insert region is 1,700 bases long and includes two genes cassettes, the second being ant (3")-Ib.
Hannecart-Pokorni
E
E
Pasteur Institute-Brussels, Belgium.
Depuydt
F
F
de wit
L
L
van Bossuyt
E
E
Content
J
J
Vanhoof
R
R
D000123:Acetyltransferases / Q000235:genetics*; D000583:Amikacin / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001530:Belgium; D016971:Citrobacter freundii / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D018432:Drug Resistance, Multiple; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006801:Humans; D007709:Klebsiella / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C091882:Sul1 protein, E coli; D000583:Amikacin; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase