title: Direct thymic involvement in anterior chamber-associated immune deviation: evidence for a nondeletional mechanism of centrally induced tolerance to extrathymic antigens in adult mice.
abstract: Recent reports have suggested that the dichotomy between central (thymic) and peripheral T cell tolerance is not absolute and that self-tolerance in perinatal animals may also involve the intrathymic generation and release to the periphery of Ag-specific immunoregulatory T cells. We have expanded this concept to include tolerance to non self Ags administered extrathymically to adult animals. In this study, we use the anterior chamber-associated immune deviation (ACAID) to demonstrate that central regulation of acquired peripheral tolerance can be induced in adult mice by the intraocular administration of low doses of nonself Ag. The results show that adult thymectomy prevents the inhibition of trinitrophenol (TNP)-specific delayed-type hypersensitivity, which normally occurs after injection of TNP-BSA into the anterior chamber (AC) of the eye. Thymocytes obtained from mice 1 to 3 days, but not 5 to 7 days, after AC injection of TNP-BSA or BSA alone specifically transfer inhibition of delayed-type hypersensitivity to mice primed with the homologous Ag. The latter observation, when correlated with the time of onset of ACAID, suggests that immunoregulatory T cells are formed in the thymus within 24 h and are exported to the peripheral lymphoid tissues between 2 and 5 days after AC injection of Ag. Immunomagnetic separation of thymocytes revealed that the immunoregulatory activity resides within the minor subset of CD4-, CD8-, TCR-alphabeta+ cells, previously postulated to induce fas ligand-mediated apoptosis and Th1 to Th2 immune deviation. Hence, the present study identifies ACAID as a prototypical model of centrally induced, nondeletional tolerance to extrathymic nonself Ags.
authors.affiliation: Department of Pathology, University of Connecticut Health Center, Farmington 06030, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: I
authors.initials: I
authors.lastname: Goldschneider
authors.forename: D
authors.initials: D
authors.lastname: Foss
authors.forename: D Y
authors.initials: DY
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: O'Rourke
authors.forename: R E
authors.initials: RE
authors.lastname: Cone
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D017637:Clonal Deletion; D000939:Epitopes / Q000032:analysis; D005260:Female; D006968:Hypersensitivity, Delayed / Q000209:etiology / Q000517:prevention & control; D007267:Injections; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000145:classification; D013934:Thymectomy; D013950:Thymus Gland / Q000276:immunology* / Q000637:transplantation; D014301:Trinitrobenzenes / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000941:Antigens; D000939:Epitopes; D014301:Trinitrobenzenes; C038601:trinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine