title: Description of an in vitro angiogenesis model designed to test antiangiogenic molecules.
abstract: Angiogenesis is involved in numerous pathologies. Studies with in vitro models allow the description and analysis of the different steps involved in this process under defined culture conditions. We describe a controllable and reproducible in vitro model. We assessed the usefulness of this model with two different cell lines: human umbilical vein endothelial cells and bovine retinal endothelial cells. These cells reorganize themselves and change their phenotypes within 24 h after seeding under our culture conditions (low human serum percentage, defined cell density, fibrin matrix) to form 'capillary-like structures' (CLS) in vitro. We showed that, depending on the cell line used, the fibrinolytic activity of the cells was a determining factor which could induce or prevent the formation of the CLS. Inhibitors of angiogenesis can be tested using such a model.
authors.affiliation: Laboratoire de Bioénergétique Fondamentale et Appliquée, Université Joseph Fourier, Grenoble, France.
authors.forename: B
authors.initials: B
authors.lastname: Vailhé
authors.forename: X
authors.initials: X
authors.lastname: Ronot
authors.forename: M
authors.initials: M
authors.lastname: Lecomte
authors.forename: N
authors.initials: N
authors.lastname: Wiernsperger
authors.forename: L
authors.initials: L
authors.lastname: Tranqui
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects*; D005342:Fibrinolysis / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D012160:Retina / Q000166:cytology / Q000187:drug effects; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects
chemical_list: D043925:Angiogenesis Inducing Agents