Structural analysis of the oligosaccharide-alditols released by reductive beta-elimination from the jelly coat of Rana utricularia eggs.
The O-linked oligosaccharides of the jelly coat surrounding the eggs of Rana utricularia were analysed by 1H-NMR spectroscopy. Comparison of their structures with those characterized from seven other amphibians confirms that the carbohydrate chains of the jelly coat mucins are markers of the species. The new sequence GlcNAc(beta1-3)GlcNAc(beta1-6)[Gal(beta1-3)]GalNAc-ol is characteristic of Rana utricularia. The presence of blood group A determinants constitutes the main feature of this mucin.
Morelle
W
W
Unité Mixte de Recherche du CNRS 111, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Strecker
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G
D000818:Animals; D001789:Blood Group Antigens / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D009077:Mucins / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry*; D010063:Ovum / Q000378:metabolism*; D011898:Ranidae; D013402:Sugar Alcohols / Q000737:chemistry*
D001789:Blood Group Antigens; D008562:Membrane Glycoproteins; D009005:Monosaccharides; D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols