title: Quinine-induced hearing loss in the guinea pig is not affected by the Ca2+ channel antagonist verapamil.
abstract: It is well documented that quinine induces reversible hearing loss and tinnitus. The purpose in this study was to induce a quinine hearing loss and to investigate if verapamil, a Ca2+ channel antagonist of L-type might affect the response. Pigmented guinea pigs (n = 24) were anaesthetized by atropine. Hypnorm and midazolam but permitting spontaneous respiration. An electrode of platinum was placed on the round window and short (10 msec) tone pulses at 8 kHz were presented to the external ear. A typical deflection of the N1-wave was determined as the hearing threshold. Quinine hydrochloride 40 mg/kg and verapamil 1 mg/kg were given intravenously. Quinine induced a significant and reversible hearing loss (mean 16 dB). This hearing loss was not at all affected by verapamil given before or after quinine. Verapamil often caused acute cardiac arrest and particularly the combination verapamil followed by quinine-induced death to the animal. We conclude that verapamil and quinine had no in vivo interaction with regard to the hearing ability.
authors.affiliation: Department of Otolaryngology, Huddinge University Hospital, Stockholm, Sweden.
authors.forename: W
authors.initials: W
authors.lastname: Jäger
authors.forename: E
authors.initials: E
authors.lastname: Idrizbegovic
authors.forename: K K
authors.initials: KK
authors.lastname: Karlsson
authors.forename: G
authors.initials: G
authors.lastname: Alván
mesh_terms: D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects; D002121:Calcium Channel Blockers / Q000009:adverse effects / Q000494:pharmacology*; D003645:Death, Sudden / Q000209:etiology; D004195:Disease Models, Animal; D004347:Drug Interactions; D006168:Guinea Pigs; D006311:Hearing Disorders / Q000139:chemically induced*; D006323:Heart Arrest / Q000139:chemically induced; D006801:Humans; D011803:Quinine / Q000009:adverse effects / Q000494:pharmacology; D014012:Tinnitus / Q000139:chemically induced; D014700:Verapamil / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D011803:Quinine; D014700:Verapamil