In vivo cytogenetic damage revealed by FISH analysis of micronuclei in uncultured human T lymphocytes.
FISH analysis of micronuclei in uncultured human T lymphocytes provides a convenient new possibility to assess structural and numerical chromosome damage in vivo. In women. T-cell micronuclei mostly contained whole chromosomes (71.6%), especially the X chromosome (28.5%). Cell culture (72 h) enhanced the frequency of micronuclei harboring acentric fragments 2.9 fold and the X chromosome 1.5 fold. X-chromosome-positive micronuclei were particularly prevalent (42.0%) in binucleate cells produced by cytochalasin B, a cytokinesis inhibitor used to identify cells that have divided in vitro. This was explained by a decrease in autosome-containing and an increase in X-positive micronuclei.
Surrallés
J
J
Department of Industrial Hygiene and Toxicology, Finnish Institute of Occupational Health, Helsinki Finland.
Falck
G
G
Norppa
H
H
D000328:Adult; D000368:Aged; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003571:Cytochalasin B / Q000494:pharmacology; D005260:Female; D006801:Humans; D048629:Micronuclei, Chromosome-Defective / Q000648:ultrastructure*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000648:ultrastructure*; D014960:X Chromosome
D003571:Cytochalasin B