T-cell-independent antiviral B cell responses in CD45-deficient mice.
CD45 is expressed on all B cells and has been reported to be essential for their B cell receptor mediated stimulation. The present study addressed T-cell-independent B cell responses in CD45-deficient mice using the glycoprotein (G) of vesicular stomatitis virus (VSV) as a model antigen. VSV-G exists in two forms exhibiting different degrees of repetitiveness. In mice, the two forms of VSV-G induce either a type 1 or a type 2 T-cell-independent B cell response. We found that CD45-deficient mice mounted T-cell-independent B cell responses to both forms of VSV-G. This demonstrates that the B cell receptor complex is able to generate a functional signal in the absence of CD45, provided the cross-linking stimulus is sufficiently strong.
Bachmann
M F
MF
Department of Medical Biophysics, Ontario Cancer Institute, Toronto, Canada.
Kündig
T M
TM
Speiser
D E
DE
McKall-Faienza
K
K
Kishara
K
K
Mak
T W
TW
Ohashi
P S
PS
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D003432:Cross-Linking Reagents; D018390:Gene Targeting; D006023:Glycoproteins / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis; D066298:In Vitro Techniques; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000378:metabolism*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D051379:Mice; D008817:Mice, Mutant Strains; D009500:Neutralization Tests; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D003432:Cross-Linking Reagents; C028391:G protein, vesicular stomatitis virus; D006023:Glycoproteins; D007075:Immunoglobulin M; D008562:Membrane Glycoproteins; D011947:Receptors, Antigen, B-Cell; D014759:Viral Envelope Proteins; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1