Cloning of biogenic amine receptors from moths (Bombyx mori and Heliothis virescens).
Based on the similarity of genes which code for guanine-nucleotide binding protein- (G-protein-) coupled receptors, cDNA clones encoding new members of the receptor family have been isolated from Bombyx mori and Heliothis virescens. The deduced protein structures exhibit highest similarity to tyramine/octopamine and serotonin receptors of Drosophila. One of the receptor clones (K50Hel) was permanently expressed in the mammalian cell line LLC-PK1. In stimulation experiments its responded to octopamine leading to an inhibition of adenylate cyclase activity in a dose-dependent manner. Pharmacological studies revealed a higher affinity for mianserin than for yohimbine suggesting, that the K50Hel clone encoded a neuronal type 3 octopamine receptor. As revealed by in situ hybridization, this receptor type is expressed in the central nervous system and antennae of moth.
von Nickisch-Rosenegk
E
E
University of Stuttgart-Hohenheim, Institute for Zoophysiology, Germany.
Krieger
J
J
Kubick
S
S
Laage
R
R
Strobel
J
J
Strotmann
J
J
Breer
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D018374:LLC-PK1 Cells; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D009655:Octopamine / Q000378:metabolism*; D017982:Receptors, Biogenic Amine / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013552:Swine
D018076:DNA, Complementary; D017982:Receptors, Biogenic Amine; C027604:norsynephrine receptor; C066175:tyramine receptor; D009655:Octopamine