Preparation of a monoclonal antibody against soybean nodule uricase (nod-35), and immunoblot analysis of the expression of nod-35 in tissues of various legumes.
Immunoblot analysis showed that uricases in non-ureide-transporting determinate nodules (Canavalia gladiata and Lotus japonicus) did not react with a monoclonal antibody against soybean nodule uricase, suggesting different immunological reactivities from those of uricases of ureide-transporting legumes.
Tajima
S
S
Dept. of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Takane
K
K
Ohkawa
K
K
Sugimoto
A
A
Okazaki
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D007887:Fabaceae / Q000378:metabolism; D015870:Gene Expression; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000276:immunology*; D010946:Plants, Medicinal; D013025:Glycine max / Q000201:enzymology*; D014407:Tumor Cells, Cultured; D014503:Urate Oxidase / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D010940:Plant Proteins; D014503:Urate Oxidase