Characterization and mapping of the Xiphophorus maculatus (Teleostei: Poeciliidae) RPS15 gene.
We have determined the nucleotide sequence and gene map location of the Xiphophorus maculatus homologue of RPS15 (ribosomal protein S15, alias RIG). The Xiphophorus RPS15 cDNA encodes 145 amino acids, which show 94% identity compared to deduced mammalian and avian RPS15 amino acid sequences. At the nucleotide level, 84% sequence identity is maintained between the fish and human gene, while homologous amphibian and avian sequences show about 80% nucleotide identity compared to the Xiphophorus sequence. Nucleotide identity substantially decreases when the fish gene is compared to Arabidopsis S15 (64%) and yeast S21 (55%) genes. Genetic linkage analysis of an RPS15 restriction fragment length polymorphism in backcross hybrids generated from the cross X. helleri x (X. maculatus Jp 163 B x X. helleri) demonstrated linkage of Xiphophorus RPS15 to the EGFR, UMPK and YES loci in Xiphophorus Linkage Group VI.
Walter
R B
RB
Department of Biology, Southwest Texas State University, San Marcos, USA.
Obermoeller
R D
RD
Moore
D D
DD
Lacson
J M
JM
Della Coletta
L
L
McEntire
B B
BB
Morizot
D C
DC
Nairn
R S
RS
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003532:Cyprinodontiformes / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
D018076:DNA, Complementary; D012269:Ribosomal Proteins; C036004:ribosomal protein S15