An unbiased analysis of V(H)-D-J(H) sequences from B-1a, B-1b, and conventional B cells.
Previous studies conclude that the repertoire of B-1a (CD5+ B) cells is highly restricted. Studies here, which use FACS sorting and single-cell PCR methodology to develop an unbiased representation of the IgH repertoires of B-1a, B-1b, and conventional B cells from the peritoneal cavity, demonstrate that the B-1a cell repertoire is more diverse than previously thought. Furthermore, adult B-1a cells have significantly fewer noncoded nucleotide (N) insertions than conventional B cells. However, B-1a cells are not defined by the absence of these regions, since such insertions are present in two-thirds of B-1a cell transcripts. All three B cell populations use a wide spectrum of V(H), D, and J(H) elements and display considerable diversity in complementarity-determining region 3 (CDR3). However, characteristic differences in the repertoires of all three B cell populations also exist, suggesting different selective and/or developmental forces act to shape each repertoire.
Kantor
A B
AB
Department of Genetics, Stanford University Medical Center, CA 94305, USA. aaron@amcell.com
Merrill
C E
CE
Herzenberg
L A
LA
Hillson
J L
JL
D000818:Animals; D000916:Antibody Diversity; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002469:Cell Separation; D005434:Flow Cytometry; D015322:Gene Rearrangement, B-Lymphocyte; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger