title: A trypsin inhibitor from snail medic seeds active against pest proteases.
abstract: A protein trypsin inhibitor from seeds of snail medic (Medicago scutellata), MsTI, has been purified by ion-exchange chromatography, gel-filtration chromatography and reverse-phase HPLC. The protein inhibits the catalytic activity of bovine beta-trypsin, with an apparent Kd of 1.8 x 10(-9), but exhibits no activity towards bovine alpha-chymotrypsin. Moreover, MsTI inhibits the trypsin-like proteinase activity present in larvae of the crop pests Adoxophyes orana, Hyphantria cunea, Lobesia botrana and Ostrinia nubilalis. The complete amino acid sequence of MsTI consists of 62 residues corresponding to a M(r) of 6925. Sequence comparison shows that MsTI exhibits significant similarity to other proteins belonging to the Bowman-Birk trypsin inhibitor family, and the closest identity (81%) with the wound-induced trypsin inhibitor from Medicago sativa leaves.
authors.affiliation: Istituto di Fisiologia Veterinaria e Biochimica, Università di Milano, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Ceciliani
authors.forename: A
authors.initials: A
authors.lastname: Tava
authors.forename: R
authors.initials: R
authors.lastname: Iori
authors.forename: M
authors.initials: M
authors.lastname: Mortarino
authors.forename: M
authors.initials: M
authors.lastname: Odoardi
authors.forename: S
authors.initials: S
authors.lastname: Ronchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002384:Catalysis; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019075:Electrophoresis, Capillary; D007915:Lepidoptera / Q000201:enzymology*; D000455:Medicago sativa / Q000737:chemistry*; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D014357:Trypsin