Monocyte chemoattractant protein-2 can exert its effects through the MCP-1 receptor (CC CKR2B).
We studied the activities of the monocyte chemoattractant proteins MCP-1, MCP-2 and MCP-3 on human embryonic kidney 293-EBNA cells transfected with the MCP-1 receptor (CC CKR2B). At 4 nM, MCP-2 induced a Ca2+ influx which was as potent as that with MCP-1 at 4 nM, although the increase by MCP-2 became saturated at higher concentrations. In addition, all three MCPs showed dose-dependent inhibition of adenylyl cyclase activity stimulated by forskolin (IC50 values: 0.3 nM for MCP-1, 7 nM for MCP-2, and 1.5 nM for MCP-3). In conclusion, our data indicate that MCP-2 can exert its effects through the MCP-1 receptor, CC CKR2B.
Yamagami
S
S
Pharmaceutical Discovery Research Laboratories, Teijin Institute for Biomedical Research, Asahigaoka, Hino, Tokyo, Japan.
Tanaka
H
H
Endo
N
N
D000067956:Adenylyl Cyclase Inhibitors; D001665:Binding Sites; D001667:Binding, Competitive; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D018932:Chemokine CCL2 / Q000378:metabolism / Q000494:pharmacology; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D005576:Colforsin / Q000494:pharmacology; D016207:Cytokines; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008024:Ligands; D018945:Monocyte Chemoattractant Proteins / Q000378:metabolism* / Q000494:pharmacology; D054390:Receptors, CCR2; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D014162:Transfection
D000067956:Adenylyl Cyclase Inhibitors; C515450:CCL7 protein, human; C515453:CCL8 protein, human; C515334:CCR2 protein, human; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D016207:Cytokines; D004791:Enzyme Inhibitors; D008024:Ligands; D018945:Monocyte Chemoattractant Proteins; D054390:Receptors, CCR2; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D011994:Recombinant Proteins; D005576:Colforsin; D002118:Calcium