Regulation of the ecdysone receptor, USP, E75 and MHR3 mRNAs by 20-hydroxyecdysone in the GV1 cell line of the tobacco hornworm, Manduca sexta.
The responsiveness of several nuclear transcription factor genes to 20-hydroxyecdysone (20E) was characterized in an embryonic cell line, GV1, from Manduca sexta. The mRNA for the Manduca ecdysone receptor (MsEcR) was present in the GV1 cells and transiently increased 2.3-fold by 5 h after the addition of 2 micrograms/ml (4 x 10(-6) M) 20-hydroxyecdysone (20E). In contrast, Manduca ultraspiracle (MsUSP) mRNA level in the GV1 cells decreased slowly to half of its initial level by 12 h when exposed to the same concentration of 20E. The mRNAs for two putative transcription factors, MsE75 and MHR3, were induced in the GV1 cells by 20E, with that for E75 appearing within 1 h whereas that for MHR3 within 2 h. The ED50S for induction of MsE75 and MHR3 gene expression in GV1 cells were 1.2 x 10(-6) and 2.5 x 10(-6) M 20E, respectively.
Lan
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Department of Zoology, University of Washington, Seattle 98195-1800, USA.
Wu
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Riddiford
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D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004441:Ecdysterone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D019476:Insect Proteins; D018402:Manduca / Q000166:cytology; D012333:RNA, Messenger; D011956:Receptors, Cell Surface / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C087173:E75 protein, insect; D019476:Insect Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D014157:Transcription Factors; C066030:USP protein, Drosophila; C055469:ecdysone receptor; D004441:Ecdysterone