[Structure and expression of the porcine skeletal muscle ryanodine receptor gene].
The ryanodine receptors (RYR) are a family of intracellular Ca2+ release channels that were first identified in the terminal cistenae of the sarcoplasmic reticulum of the skeletal and cardiac muscle. Mutations within the skeletal muscle isoform were shown to cause malignant hyperthermia in swine and man. We have analysed the genomic structure of the porcine skeletal muscle ryanodine receptor and its expression using chimeric reporter gene constructs consisting of the RYR1 gene promoter and the chloramphenicol acetyltransferase gene after transfection in muscle and non-muscle cells.
Brenig
B
B
Tierärztlichen Institut, Universität Göttingen.
Schmoelzl
S
S
Leeb
T
T
Wen
G
G
Reinhart
B
B
Baum-Gartner
B
B
Krempler
A
A
D000818:Animals; D001483:Base Sequence; D015220:Calcium Channels / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry; D015870:Gene Expression; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D019837:Ryanodine Receptor Calcium Release Channel; D013552:Swine; D014162:Transfection
D015220:Calcium Channels; D009124:Muscle Proteins; D019837:Ryanodine Receptor Calcium Release Channel; D004247:DNA