title: Markers of type D retroviruses in children with Burkitt's-type lymphoma.
abstract: Antibodies to gag-coded proteins of type D retroviruses have been detected in children with lymphadenopathy [1]. We tested 41 HIV noninfected children with lymphoproliferative diseases (27 cases of Burkitt's-type lymphoma, six cases of Hodgkin's disease, four cases of T-cell lymphoma, three cases of lymphoblastic lymphoma and one case of large-cell anaplastic lymphoma) for the presence of type D retroviral serological and genetical markers. Twenty-five healthy donors were tested as a control. DNA samples from peripheral blood lymphocytes were analyzed by the polymerase chain reaction (PCR) and Southern blotting for the presence of type D retroviral related sequences. MPMV pro-pol specific sequences have been detected in 18 out of 27 children with Burkitt's-type lymphoma. By means of Western blotting, six patients positive in PCR/Southern blotting analysis were also found to contain Mason-Pfizer monkey virus (MPMV) specific antibodies, in their sera. All children with other lymphoproliferative diseases as well as healthy donors were negative in PCR/Southern blotting and Western blotting analysis. These data suggest the possible association of type D retroviral markers with Burkitt's-type lymphoma of children.
authors.affiliation: Cancer Virus Immunology Laboratory, Blokhin Cancer Research Center of RAMS, Moscow, Russia.
authors.forename: J G
authors.initials: JG
authors.lastname: Kzhyshkowska
authors.forename: A V
authors.initials: AV
authors.lastname: Kiselev
authors.forename: G A
authors.initials: GA
authors.lastname: Gordina
authors.forename: V I
authors.initials: VI
authors.lastname: Kurmashow
authors.forename: N M
authors.initials: NM
authors.lastname: Portjanko
authors.forename: A S
authors.initials: AS
authors.lastname: Ostashkin
authors.forename: K V
authors.initials: KV
authors.lastname: Ilyin
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000032:analysis / Q000302:isolation & purification; D015139:Blotting, Southern; D015153:Blotting, Western; D002051:Burkitt Lymphoma / Q000209:etiology / Q000821:virology*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D006689:Hodgkin Disease / Q000209:etiology / Q000821:virology; D006801:Humans; D008214:Lymphocytes; D017728:Lymphoma, Large-Cell, Anaplastic / Q000209:etiology / Q000821:virology; D016399:Lymphoma, T-Cell / Q000209:etiology / Q000821:virology; D008232:Lymphoproliferative Disorders / Q000209:etiology / Q000821:virology; D008297:Male; D016093:Mason-Pfizer monkey virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000209:etiology / Q000821:virology; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral