Molecular characterization of two species-specific tandemly repeated DNAs from entomopathogenic nematodes Steinernema and Heterorhabditis (Nematoda:Rhabditida).
Two AluI tandemly repeated DNAs were cloned from two entomopathogenic nematodes: the first one from Steinernema glaseri and the second one from Heterorhabditis bacteriophora. The monomeric units of these two satellite DNAs have a repeat length of 174 and 168 bp, respectively. These AluI repeated element families appear to constitute 5.5% of the S. glaseri genome and 5% of the H. bacteriophora genome. Their A + T contents were estimated at 55% and 57%. Moreover, the monomers of these two families are quite homogeneous in sequence, showing, on average, 3.9% and 2.7% divergence from their respective consensus sequence. These results suggest that some mechanism is acting to maintain the homogeneity of these repeated DNAs despite their abundance. We have also shown that these two DNA families are species-specific and therefore could be used for the identification of Steinernema and Heterorhabditis entomopathogenic nematode species.
Grenier
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E
Laboratoire de Biologie des Invertébrés, INRA, BP 2078, Antibes, France.
Laumond
C
C
Abad
P
P
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018509:DNA, Helminth / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D007313:Insecta / Q000469:parasitology*; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D012091:Repetitive Sequences, Nucleic Acid; D017172:Rhabditoidea / Q000235:genetics* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D018509:DNA, Helminth; D004276:DNA, Satellite