title: In-frame cloning of synthetic genes using PCR inserts.
authors.affiliation: Department of Biological Science, Philadelphia College of Pharmacy and Science, PA, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Pierce
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015195:Drug Design; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004926:Escherichia coli; D005813:Genes, Synthetic; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D013379:Substrate Specificity; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes