Optimization of the production of a bacteriocin from Haloferax mediterranei Xia3.
The optimal conditions for the production of the halocin H1, a 31 kDa bacteriocin-like molecule produced by the extreme halophilic Archaea Haloferax mediterranei Xia3 active against Gram-negative haloarchaea, was characterized. The physico-chemical conditions required for the optimal production of halocin H1 are similar to those found in the habitat in which the microorganism was isolated: 20% salt concentration and temperature range between 37 and 42 degrees C. Optimal antimicrobial activity was obtained using 0.5% of N-Z amine E as nutrient.
Platas
G
G
Centro de Biología Molecular, Universidad Autónoma de Madrid, Cantoblanco, España. gonzalo_platas@merck.com
Meseguer
I
I
Amils
R
R
D001430:Bacteriocins / Q000096:biosynthesis* / Q000302:isolation & purification; D003470:Culture Media / Q000494:pharmacology; D016931:Halobacteriales / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D001430:Bacteriocins; D003470:Culture Media; D012965:Sodium Chloride