Elaidic and trans-vaccenic acids in plasma phospholipids as indicators of dietary intake of 18:1 trans-fatty acids.
Octadecenoic (18:1) trans-fatty acid fractions from margarine, butter and plasma phospholipids (PL) were isolated by silver ion TLC, and nine positional isomers (n-11-n-3) were identified by GC-MS based on their ozonolysis products. The GC analysis of the isolated fractions gave similar peak profiles and separated seven trans-isomers (n-11-n-6 and n-3). Without a preceding isolation step, the reproducibility of the GC method for plasma PL elaidic (18:1 n 9 trans) and trans-vaccenic acids (n-7) was 3.4 and 2.7% (R.S.D.), respectively. These trans-isomers were rapidly incorporated and cleared in plasma PL and they closely reflected both increased and decreased intake of 18:1 trans-fatty acids during moderate fat substitutions. Significant associations between high-density lipoprotein cholesterol (HDL-C) and PL elaidic and trans-vaccenic acids appeared in habitual margarine users only.
Seppänen-Laakso
T
T
Department of Pharmacy, FIN-00014 University of Helsinki, Finland.
Laakso
I
I
Backlund
P
P
Vanhanen
H
H
Viikari
J
J
D000328:Adult; D002079:Butter; D002855:Chromatography, Thin Layer; D004041:Dietary Fats / Q000008:administration & dosage* / Q000097:blood; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008383:Margarine; D019301:Oleic Acid / Q000097:blood*; D009829:Oleic Acids / Q000097:blood*; D000069463:Olive Oil; D010743:Phospholipids / Q000097:blood* / Q000737:chemistry; D010938:Plant Oils
D004041:Dietary Fats; D009829:Oleic Acids; D000069463:Olive Oil; D010743:Phospholipids; D010938:Plant Oils; D019301:Oleic Acid; C011459:elaidic acid; D002079:Butter; D008383:Margarine; C050413:11-octadecenoic acid