title: Transformation of the mycotoxin ochratoxin A in plants: 1. Isolation and identification of metabolites formed in cell suspension cultures of wheat and maize.
abstract: The metabolism of the mycotoxin ochratoxin A in plant cells was investigated using cell suspension cultures of wheat and maize. A number of metabolites were detected by HPLC-chromatography with fluorescence detection. The main metabolites were ochratoxin alpha, ochratoxin A methyl ester, two isomers of hydroxyochratoxin A, and the glucosides and methyl esters of both hydroxyochratoxin A isomers. The compounds were isolated by TLC and preparative HPLC and identified by mass spectrometry and specific enzymic reactions.
authors.affiliation: Bayerische Landesanstalt für Ernährung, Abteilung Ernährung, München, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Ruhland
authors.forename: G
authors.initials: G
authors.lastname: Engelhardt
authors.forename: W
authors.initials: W
authors.lastname: Schäfer
authors.forename: P R
authors.initials: PR
authors.lastname: Wallnöfer
mesh_terms: D001711:Biotransformation; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D009183:Mycotoxins / Q000493:pharmacokinetics*; D009793:Ochratoxins / Q000493:pharmacokinetics*; D011874:Radiometry; D013050:Spectrometry, Fluorescence; D014908:Triticum / Q000166:cytology / Q000378:metabolism*; D003313:Zea mays / Q000166:cytology / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A