title: The relationships among IGF-1, DNA content, and protein accumulation during skeletal muscle hypertrophy.
abstract: Insulin-like growth factor-1 (IGF-1) is known to have anabolic effects on skeletal muscle cells. This study examined the time course of muscle hypertrophy and associated IGF-1 peptide and mRNA expression. Data were collected at 3, 7, 14, and 28 days after surgical removal of synergistic muscles of both normal and hypophysectomized (HX) animals. Overloading increased the plantaris (Plant) mass, myofiber size, and protein-to-body weight ratio in both groups (normal and HX; P < 0.05). Muscle IGF-1 peptide levels peaked at 3 (normal) and 7 (HX) days of overloading with maximum 4.1-fold (normal) and 6.2-fold (HX) increases. Increases in muscle IGF-1 preceded the hypertrophic response. Total DNA content of the overloaded Plant increased in both groups. There was a strong positive relationship between IGF-1 peptide and DNA content in the overloaded Plant from both groups. These results indicate that 1) the muscles from rats with both normal and severely depressed systemic levels of IGF-1 respond to functional overload with an increase in local IGF-1 expression and 2) this elevated IGF-1 may be contributing to the hypertrophy response, possibly via the mobilization of satellite cells to provide increases in muscle DNA.
authors.affiliation: Department of Physiology and Biophysics, University of California, Irvine 92697, USA.
authors.forename: G R
authors.initials: GR
authors.lastname: Adams
authors.forename: F
authors.initials: F
authors.lastname: Haddad
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism*; D005260:Female; D006984:Hypertrophy / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000473:pathology*; D011506:Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009124:Muscle Proteins; D011506:Proteins; D007334:Insulin-Like Growth Factor I; D004247:DNA