title: Molecular cloning of the nemA gene encoding N-ethylmaleimide reductase from Escherichia coli.
abstract: Using the gene mapping membrane technique, we identified a gene (nemA) that encodes N-ethylmaleimide reductase in Escherichia coli. The open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39,514 Da. The deduced amino acid sequence showed a high degree of homology (87% identical) with the pentaerythritol tetranitrate reductase of Enterobacter cloacae and the morphinone reductase of Pseudomonas putida (52% identical).
authors.affiliation: Department of Pharmaceutical Sciences, Tohoku University Hospital, Aoba-ku, Sendai, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: Y
authors.initials: Y
authors.lastname: Tomioka
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Yonezawa
authors.forename: T
authors.initials: T
authors.lastname: Hishinuma
authors.forename: M
authors.initials: M
authors.lastname: Mizugaki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005033:Ethylmaleimide / Q000378:metabolism*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*
chemical_list: D010088:Oxidoreductases; D005033:Ethylmaleimide