title: The precursor protein of the structural apolipoproteins of lipophorin: cDNA and deduced amino acid sequence.
abstract: A 10 138 bp cDNA from the fat body of the tobacco hornworm, Manduca sexta, which encodes the precursor protein for apolipophorin aPOLP-1 and -II, the structural apolipoproteins of the insect lipoprotein, lipophorin, has been cloned and sequenced. The cDNA has a single 9915 bp open reading frame beginning at an initiating ATG at bp 59 and extending to a stop codon at position 9974. This open reading frame encodes a 3305 amino acid protein with a molecular mass of 366 812 Da. Signal peptide cleavage is predicted to occur after residue 23, leaving a 3,282 amino acid precursor protein. The precursor protein is arranged with apoLp-II at the amino terminal end and apoLp-I at the carboxy terminal end. At present, the site of cleavage of the precursor protein to generate apoLp-I and -II is unknown.
authors.affiliation: Department of Biochemistry, University of Arizona, Tucson 85721, USA.
authors.forename: K
authors.initials: K
authors.lastname: Sundermeyer
authors.forename: J K
authors.initials: JK
authors.lastname: Hendricks
authors.forename: S V
authors.initials: SV
authors.lastname: Prasad
authors.forename: M A
authors.initials: MA
authors.lastname: Wells
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D018076:DNA, Complementary; D008074:Lipoproteins / Q000235:genetics*; D018402:Manduca / Q000378:metabolism*; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D001053:Apolipoproteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D008074:Lipoproteins; D011498:Protein Precursors; C032830:lipophorin