The structure of the muscle protein complex 4Ca2+.troponin C.troponin I. Monte Carlo modeling analysis of small-angle X-ray data.
Analysis of scattering data based on a Monte Carlo integration method was used to obtain a low resolution model of the 4Ca2+.troponin C.troponin I complex. This modeling method allows rapid testing of plausible structures where the best fit model can be ascertained by a comparison between model structure scattering profiles and measured scattering data. In the best fit model, troponin I appears as a spiral structure that wraps around 4Ca2+.troponin C which adopts an extended dumbbell conformation similar to that observed in the crystal structures of troponin C. The Monte Carlo modeling method can be applied to other biological systems in which detailed structural information is lacking.
Olah
G A
GA
Chemical Science and Technology Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Trewhella
J
J
D000818:Animals; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008962:Models, Theoretical; D009010:Monte Carlo Method; D018482:Muscle, Skeletal / Q000502:physiology; D009502:Neutrons; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012542:Scattering, Radiation; D019209:Troponin C / Q000737:chemistry* / Q000378:metabolism; D019210:Troponin I / Q000737:chemistry* / Q000378:metabolism; D014961:X-Ray Diffraction / Q000379:methods
D019209:Troponin C; D019210:Troponin I; D002118:Calcium