title: DNA sequencing using a four-color confocal fluorescence capillary array scanner.
abstract: The design, construction and operation of a four-color capillary array electrophoresis scanner are presented. The use of sensitive energy transfer primers facilitates four-color detection of the DNA sequencing fragments following excitation at a single laser wavelength (488 nm). This scanner collects fluorescence data from up to 25 capillaries in parallel. The resulting four-color image files are automatically reduced to four-color line plots, and a base-calling program (Sax) is used to call the sequence. The performance of this system for DNA sequencing is demonstrated by examining twelve different motifs of the hypervariable region I of human mitochondrial (mt) DNA obtained from a Sierra Leone population.
authors.affiliation: Department of Chemistry, School of Public Health, University of California, Berkeley 94720, USA.
authors.forename: I
authors.initials: I
authors.lastname: Kheterpal
authors.forename: J R
authors.initials: JR
authors.lastname: Scherer
authors.forename: S M
authors.initials: SM
authors.lastname: Clark
authors.forename: A
authors.initials: A
authors.lastname: Radhakrishnan
authors.forename: J
authors.initials: J
authors.lastname: Ju
authors.forename: C L
authors.initials: CL
authors.lastname: Ginther
authors.forename: G F
authors.initials: GF
authors.lastname: Sensabaugh
authors.forename: R A
authors.initials: RA
authors.lastname: Mathies
mesh_terms: D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods; D012807:Sierra Leone
chemical_list: D004272:DNA, Mitochondrial