title: Volatile general anaesthetic actions on recombinant nACh alpha 7, 5-HT3 and chimeric nACh alpha 7-5-HT3 receptors expressed in Xenopus oocytes.
abstract: The effect of halothane and isoflurane was studied on the function of recombinant neurotransmitter receptors expressed in Xenopus oocytes. Both anaesthetics inhibited nicotinic acetylcholine type alpha 7 (nACh alpha 7) receptor-mediated responses, potentiated 5-hydroxytryptamine type 3 (5-HT3) receptor-mediated responses at low agonist concentrations, and inhibited the function of a chimeric receptor (with the N-terminal domain from the nACh alpha 7 receptor and the transmembrane and C-terminal domains from the 5-HT3 receptor) in a manner similar to that of the nACh alpha 7 receptor. Since the N-terminal domain of the chimeric receptor was from the nACh alpha 7 receptor, the observations suggest that the inhibition involves the N-terminal domain of the receptor.
authors.affiliation: Laboratory of Molecular & Cellular Neurobiology, National Institute on Alcohol Abuse & Alcoholism, National Institutes of Health, Bethesda, MD 20892-8205, USA.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Oz
authors.forename: R R
authors.initials: RR
authors.lastname: Stewart
authors.forename: R W
authors.initials: RW
authors.lastname: Peoples
authors.forename: F F
authors.initials: FF
authors.lastname: Weight
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D018680:Cholinergic Antagonists / Q000494:pharmacology*; D006221:Halothane / Q000494:pharmacology; D007530:Isoflurane / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000187:drug effects*; D011985:Receptors, Serotonin / Q000187:drug effects*; D011994:Recombinant Proteins / Q000378:metabolism; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*; D014981:Xenopus
chemical_list: D018685:Anesthetics, Inhalation; D018680:Cholinergic Antagonists; D011950:Receptors, Cholinergic; D011985:Receptors, Serotonin; D011994:Recombinant Proteins; D017366:Serotonin Receptor Agonists; D007530:Isoflurane; D006221:Halothane