title: A chromosome-specific microdissected library increases marker density on bovine chromosome 1.
abstract: Genetic resolution of bovine chromosome 1 (BTA1) linkage group was significantly increased by screening for microsatellite clones a microdissected library constructed from a bovine cell line carrying a t(1;29) translocation. Eighty-five percent of the microsatellites (ms) (46/54) identified were informative in the USDA/MARC mapping population, and 96% of these ms (44/46) linked to BTA1 (LOD > 3.0). When merged with 40 existing BTA1 markers the genetic map spanned 153.8 cM (sex-averaged interval, 1.9 cM). The fourfold improvement in marker density of BTA1 provides a genetic map that enhances mapping of quantitative trait loci and implementation of marker assisted selection.
authors.forename: T S
authors.initials: TS
authors.lastname: Sonstegard
authors.forename: F
authors.initials: F
authors.lastname: Abel Ponce de León
authors.forename: C W
authors.initials: CW
authors.lastname: Beattie
authors.forename: S M
authors.initials: SM
authors.lastname: Kappes
mesh_terms: D000818:Animals; D002417:Cattle; D002875:Chromosomes / Q000235:genetics*; D005819:Genetic Markers; D015698:Genomic Library
chemical_list: D005819:Genetic Markers