title: Altered organic anion and osmolyte content and excretion in rat polycystic kidney disease: an NMR study.
abstract: Polycystic kidney disease (PKD) is the fourth most common cause of end-stage renal disease and the most common potentially lethal inherited disease in humans. Early identification of carriers of dominant PKD in the absence of genetic markers is problematic in both humans and the Han:SPRD-cy/+ rat, a model of PKD that shares many features of human disease. We undertook a proton magnetic resonance imaging (MRI) study of young Han:SPRD-cy/+ and unaffected Han:SPRD(-)+/+ animals to determine whether carrier status could be identified based upon image appearance or signal characteristics. Affected animals demonstrated significant prolongation of longitudinal relaxation time (T1) and transverse relaxation time (T2) in both cystic renal cortex and noncystic renal medulla. Both of these measurements correlated significantly with whole kidney section tubular luminal space measurements, a correlate of water space, in the renal cortex, but only T1 in renal medulla showed a relationship to tubular luminal volume measured throughout the kidney. Urine and perchloric acid kidney extracts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy to test the hypothesis that imaging differences implied specific urinary and tissue biochemical differences between affected and normal animals. 1H-NMR spectra of urine from cy/+ animals showed significantly increased excretion of alanine, citrate, succinate, and, 2-oxoglutarate but not methylamine compounds compared with +/+ animals. 1H-NMR spectra of aqueous perchloric acid kidney extracts confirmed reduced concentrations of the above ions and others involved in the citric acid cycle, as well the osmolytes betaine, taurine, and glycerophosphocholine PKD in the Han:SPRD-cy/+ rat is associated with distinct early MRI changes and alterations in urinary and tissue levels of organic anions and osmolytes.
authors.affiliation: Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, Canada.
authors.forename: M R
authors.initials: MR
authors.lastname: Ogbron
authors.forename: S
authors.initials: S
authors.lastname: Sareen
authors.forename: J
authors.initials: J
authors.lastname: Prychitko
authors.forename: R
authors.initials: R
authors.lastname: Buist
authors.forename: J
authors.initials: J
authors.lastname: Peeling
mesh_terms: D000818:Animals; D000838:Anions / Q000378:metabolism*; D006580:Genetic Carrier Screening / Q000379:methods; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009994:Osmolar Concentration; D007690:Polycystic Kidney Diseases / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011922:Rats, Mutant Strains; D014556:Urine / Q000737:chemistry
chemical_list: D000838:Anions