Recombinant fibre proteins of human adenoviruses Ad9, Ad15 and Ad19: localization of the haemagglutination properties and the type-specific determinant.
The adenovirus fibre carries the type-specific gamma determinant the existence of which was suggested by haemagglutination inhibition tests. Furthermore, the fibre is thought to be responsible for the agglutination of monkey, rat and human erythrocytes. In order to verify that the haemagglutination properties and the type-specific gamma determinant are located on the fibre knob domain, several recombinant fibre proteins of subgenus D adenoviruses were constructed, expressed in HeLa cells and tested in haemagglutination and haemagglutination inhibition tests. Our data showed that the epitopes responsible for the interaction with erythrocytes and the gamma determinant are located on the fibre knob domain.
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Institut für Virologie und Seuchenhygiene, Medizinische Hochschule Hannover, Germany.
Adrian
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Pring-Akerblom
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D000260:Adenoviruses, Human / Q000276:immunology*; D000818:Animals; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D000939:Epitopes / Q000032:analysis; D006367:HeLa Cells; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000032:analysis*; D006801:Humans; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000276:immunology
D036022:Capsid Proteins; D000939:Epitopes; D006389:Hemagglutinins, Viral; D011993:Recombinant Fusion Proteins; C018315:hexon capsid protein, Adenovirus