Growth of hepatitis A virus in murine cells.
In order to investigate the growth of hepatitis A virus (HAV) in murine cells, L929 cells of the established mouse cell line were transfected with the virion RNA or infected with the virions and examined for the formation of negative-strand RNA and the rise of the viral infectivity titer. In both the transfected and infected cells, the formation of negative-strand HAV RNA was assayed by the reverse transcription-polymerase chain reaction (RT-PCR). In the transfected cells, infectious HAV of an average titer of 10(1.8) TCID50/dish was obtained. The experiment with the virion infection was further extended by using other mouse cell lines, namely Balb/3T3 clone A31, NIH/3T3, and Swiss/3T3. Here, only NIH/3T3 cells were found capable to support the formation of negative-strand HAV RNA. Thus some murine cell lines are considered to have a complete cellular machinery for supporting the growth of HAV, though the efficiency of virus growth therein was considerably lower as compared to that in the susceptible primate cells.
Takeda
Y
Y
Department of Virology, Niigata University School of Medicine, Japan.
Ashida
M
M
Hamada
C
C
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D006507:Hepatovirus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral / Q000032:analysis; D014162:Transfection; D014771:Virion; D014776:Virus Cultivation
D012367:RNA, Viral