Determination of 5-aminolaevulinic acid dehydratase activity in erythrocytes and porphobilinogen in urine by micellar electrokinetic capillary chromatography.
A micellar electrokinetic capillary chromatographic (MECC) method has been developed and optimised for the separation of 5-aminolaevulinic acid (ALA) and porphobilinogen (PBG). The running buffer consisted of a mixture of 20 mM sodium phosphate and 20 mM sodium borate containing 50 mM sodium dodecyl sulphate (SDS) adjusted to pH 9.5 with 1 M NaOH. The running voltage and temperature were 20-25 kV and 30 degrees C, respectively. The MECC method for the analysis of PBG is fast and simple and is useful for the screening of PBG in the urine of patients suspected to have acute intermittent porphyria (AIP), and for the confirmation of lead exposure by measuring red-cell ALA-dehydratase (ALA-D) activity with ALA as the enzyme substrate.
Luo
J L
JL
MRC Toxicology Unit, University of Leicester, UK.
Deka
J
J
Lim
C K
CK
D000622:Aminolevulinic Acid / Q000378:metabolism; D019075:Electrophoresis, Capillary; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007855:Lead Poisoning / Q000097:blood / Q000201:enzymology; D008823:Micelles; D011162:Porphobilinogen / Q000652:urine*; D000623:Porphobilinogen Synthase / Q000097:blood*; D017118:Porphyria, Acute Intermittent / Q000652:urine; D013056:Spectrophotometry, Ultraviolet
D008823:Micelles; D011162:Porphobilinogen; D000622:Aminolevulinic Acid; D000623:Porphobilinogen Synthase