APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle.
The molecular mechanisms that link cell-cycle controls to the mitotic apparatus are poorly understood. A component of the Saccharomyces cerevisiae spindle, Ase1, was observed to undergo cell cycle-specific degradation mediated by the cyclosome, or anaphase promoting complex (APC). Ase1 was degraded when cells exited from mitosis and entered G1. Inappropriate expression of stable Ase1 during G1 produced a spindle defect that is sensed by the spindle assembly checkpoint. In addition, loss of ASE1 function destabilized telophase spindles, and expression of a nondegradable Ase1 mutant delayed spindle disassembly. APC-mediated proteolysis therefore appears to regulate both spindle assembly and disassembly.
Juang
Y L
YL
Department of Pediatric Oncology, The Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
Huang
J
J
Peters
J M
JM
McLaughlin
M E
ME
Tai
C Y
CY
Pellman
D
D
D000705:Anaphase; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism*; D016193:G1 Phase; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008938:Mitosis; D008969:Molecular Sequence Data; D009024:Morphogenesis; D016297:Mutagenesis, Site-Directed; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D013692:Telophase
D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins