title: Antibodies to maitotoxin elicited by immunization with toxin-fragment conjugates.
abstract: Maitotoxin is a water-soluble polyether toxin produced by the benthic dinoflagellate, Gambierdiscus toxicus. Toxin fragments generated by periodate oxidation were conjugated to keyhole limpet hemocyanin, and the resulting immunogens were used to elicit maitotoxin-specific antibodies in mice. A competitive immunoassay developed with polyclonal IgG antibodies detected approximately 3 nM purified maitotoxin standard (IC50 approximately 13 nM; 45 ng/ml) but did not detect other polyether marine toxins. These are the first reported maitotoxin-specific antibodies.
authors.affiliation: Hawaii Biotechnology Group, Inc., Aiea 96701, USA.
authors.forename: G S
authors.initials: GS
authors.lastname: Bignami
authors.forename: D F
authors.initials: DF
authors.lastname: Waller
authors.forename: T
authors.initials: T
authors.lastname: Yasumoto
mesh_terms: D000818:Animals; D000992:Antitoxins / Q000096:biosynthesis*; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000096:biosynthesis; D000922:Immunotoxins / Q000276:immunology*; D008387:Marine Toxins / Q000276:immunology*; D051379:Mice; D035964:Oxocins; D010446:Peptide Fragments / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D000095702:Polyether Toxins
chemical_list: D000992:Antitoxins; D002352:Carrier Proteins; D006433:Hemocyanins; D007074:Immunoglobulin G; D000922:Immunotoxins; D008387:Marine Toxins; D035964:Oxocins; D010446:Peptide Fragments; D012710:Serum Albumin, Bovine; C020394:maitotoxin; D000095702:Polyether Toxins; C032808:keyhole-limpet hemocyanin