Murine cytomegalovirus inactivated by sodium periodate is innocuous and immunogenic in mice and protects them against death and infection.
Sodium periodate (10 mM, 4 degrees C) inactivated murine cytomegalovirus (MCMV) very rapidly (loss of 2 to 3 log of viral infectivity per minute). Periodate-treated MCMV (PI-MCMV) was shown to be innocuous in mice, as determined by the inability of the virus to replicate. PI-MCMV induced a strong humoral immune response, with a high level of neutralizing antibodies. Mice immunized with PI-MCMV were protected against death and infection, when a lethal challenge with the virulent virus was administered 3 weeks after immunization and from death but not infection when virulent virus was administered at 3 months. Finally, no reactivation of potentially latent challenge virus (sublethal dose at 3 weeks) was observed in animals immunosuppressed at 6 months after immunization. Taken together, these results suggest that periodate could serve as an inactivating agent to prepare killed vaccines.
Geoffroy
F
F
Laboratoire d'immunochimie, Faculté de Médecine Lyon-Sud, Oullins, France.
Moachon
N
N
Rodwell
J
J
Quash
G A
GA
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000517:prevention & control*; D005260:Female; D007114:Immunization; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018146:Muromegalovirus / Q000187:drug effects* / Q000276:immunology / Q000472:pathogenicity; D010504:Periodic Acid; D015164:Vaccines, Inactivated; D014774:Virulence; D017735:Virus Latency; D014779:Virus Replication
D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D010504:Periodic Acid; C009288:metaperiodate