Arterial wall mechanical characteristics after treatment in collagenase: an in vitro aneurysm model.
OBJECTIVE
To investigate the mechanical characteristics of canine aortas treated with buffered collagenase as a first step in developing an animal model of aortic aneurysm for the validation of stent-grafts.


METHODS
In vitro study of canine aortas.


METHODS
Canine thoraco-abdominal arteries were divided into the descending thoracic aorta the suprarenal artery and the infrarenal artery; these segments were incubated separately in a buffered collagenase solution for 1 to 6 hours. Some segments were left untreated as controls.


METHODS
Mean arterial wall thickness, measured with Vernier callipers and computerized histomorphometric methods and longitudinal tensile strength of control and treated vessel segments.


RESULTS
The arterial wall thickness decreased with incubation time. After 1 hour of incubation the reduction was approximately 15% for the descending thoracic aorta, 16% for the suprarenal artery and 18% for the infrarenal artery. After 6 hours the total reduction in wall thickness was 32%, 41% and 44% respectively for the 3 segments. The tensile strength of the treated arterial segments also decreased with the incubation period. Initially, the infrarenal segment displayed the greatest strength; however, this was reversed as the period of incubation increased. The inelastic limit of the descending thoracic aorta control segment was reached at 100% elongation, whereas that of the suprarenal artery was reached at 80% and that of the infrarenal artery was reached at 60% elongation. All of the arterial segments became weaker as the period of incubation in buffered collagenase increased.


CONCLUSIONS
This in vitro incubation technique successfully altered the structure of the collagen fibre network within the arterial wall. This method may be an option in developing an aneurysm model to test stent-grafts in vivo.
Dadgar
L
L
Department of Surgery, Université Laval, Quebec, Que.
Marois
Y
Y
Deng
X
X
Guidoin
R
R
D000783:Aneurysm; D000818:Animals; D001013:Aorta, Thoracic / Q000033:anatomy & histology / Q000187:drug effects; D001158:Arteries / Q000033:anatomy & histology* / Q000187:drug effects*; D017364:Collagenases / Q000494:pharmacology*; D004195:Disease Models, Animal; D004285:Dogs; D066298:In Vitro Techniques; D012077:Renal Artery / Q000033:anatomy & histology / Q000187:drug effects; D015203:Reproducibility of Results; D015607:Stents; D013718:Tensile Strength
D017364:Collagenases