Myosin binding subunit of smooth muscle myosin phosphatase at the cell-cell adhesion sites in MDCK cells.
We examined the intracellular localization of the myosin binding subunit (MBS) of smooth muscle myosin phosphatase. In MDCK cells in a confluent monolayer of polarized epithelial sheet, MBS was concentrated to the cell-cell adhesion sites. Double-immunofluorescence analysis with anti-MBS and anti-beta-catenin antibodies showed that MBS was mainly localized at the adherens junction. Furthermore, MBS was translocated reversibly between the cytosol and the cell-cell adhesion sites during the formation and disappearance of cell-cell contacts. These data suggest that MBS may play an important role in the regulation of the cell-cell adhesion.
Inagaki
N
N
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan.
Nishizawa
M
M
Ito
M
M
Fujioka
M
M
Nakano
T
T
Tsujino
S
S
Matsuzawa
K
K
Kimura
K
K
Kaibuchi
K
K
Inagaki
M
M
D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry* / Q000502:physiology; D002448:Cell Adhesion; D002450:Cell Communication; D002460:Cell Line; D016764:Cell Polarity; D002645:Chickens; D004285:Dogs; D009130:Muscle, Smooth / Q000201:enzymology* / Q000502:physiology; D043263:Myosin-Light-Chain Phosphatase; D009218:Myosins / Q000737:chemistry* / Q000502:physiology; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000502:physiology; D051381:Rats
D002352:Carrier Proteins; D010749:Phosphoprotein Phosphatases; D043263:Myosin-Light-Chain Phosphatase; D009218:Myosins