Haloanaerobium congolense sp. nov., an anaerobic, moderately halophilic, thiosulfate- and sulfur-reducing bacterium from an African oil field.
A strictly anaerobic, moderately halophilic, Gram-negative, non-motile rod-shaped bacterium was isolated from an oil-well head sample of an offshore Congolese oil field. The strain, designated SEBR 4224T (T = type strain), grew optimally at 42 degrees C and pH 7.0 in a complex medium containing 10% NaCl with a generation time of 2.5 h. Strain SEBR 4224T grew on a range of carbohydrates including fructose, galactose, D-glucose, maltose, D-mannose, D-ribose, sucrose, and trehalose. Yeast extract and/or bio-Trypcase was required for growth on carbohydrates and could not be replaced with amino acids and/or vitamins. The end-products from glucose fermentation were acetate, H2, and CO2. Thiosulfate and elemental sulfur were used as electron acceptors. Thiosulfate improved carbohydrate utilization and biomass yields. The G + C content of the isolate was 34 mol%. Ribosomal 16S rRNA sequence analysis showed that strain SEBR 4224T is a new member of the genus Haloanaerobium. The lack of DNA homology with H. acetoethylicum, its closest relative, as determined by DNA-DNA hybridization supports the designation of strain SEBR 4224T as a new species, Haloanaerobium congolense sp. nov. The type strain is SEBR 4224T (= DSM 11287).
Ravot
G
G
Laboratoire ORSTOM de Microbiologie Anaérobies, Université de Provence, CESB-ESIL, Marseille, France.
Magot
M
M
Ollivier
B
B
Patel
B K
BK
Ageron
E
E
Grimont
P A
PA
Thomas
P
P
Garcia
J L
JL
D001431:Bacteriological Techniques; D050260:Carbohydrate Metabolism; D002473:Cell Wall / Q000648:ultrastructure; D002965:Classification; D003223:Congo; D003596:Cytosine; D004269:DNA, Bacterial / Q000032:analysis; D005285:Fermentation; D005838:Genotype; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D006147:Guanine; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009821:Oils; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012492:Salts / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013455:Sulfur / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism*
D004269:DNA, Bacterial; D009821:Oils; D012336:RNA, Ribosomal, 16S; D012492:Salts; D013885:Thiosulfates; D006147:Guanine; D013455:Sulfur; D003596:Cytosine