Sequencing, analysis and expression in Escherichia coli of a gene encoding a 15 kDa Cryptosporidium parvum protein.
A previous paper presented data on a cDNA sequence encoding a protein associated with the AIDS related pathogen, Cryptosporidium parvum. However, the position of the start codon was uncertain, and the 5' end was continuous, lending doubt about the size and complete sequence of the final protein product. Herein we present the complete gene sequence and conclude the predicted size of the putative protein to be 16.2 kDa.
Khramtsov
N V
NV
Division of Biology, Kansas State University, Manhattan 66506, USA.
Oppert
B
B
Montelone
B A
BA
Upton
S J
SJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016785:Cryptosporidium parvum / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification
D015800:Protozoan Proteins; D011994:Recombinant Proteins