[Use of aerosol A-300 amd GF/F (GF/C) filters for purifying fragments of DNA, plasmid DNA, and RNA].
Aerosil A-300 and GF/F (GF/C) filters were used for purifying plasmid DNA and intact RNA as well as for the recovery of DNA fractionated on agarose gels. In the presence of guanidinium thiocyanate Aerosil A-300 and GF/F (GF/C) filters selectively bind nucleic acids, while proteins, agarose polysaccharides and salts are washed off. Nucleic acids desorbed from the Aerosil and filters are available immediately as substrates: DNA for restriction analysis, ligation and sequencing; RNA--for RT-PCR.
Gribanov
P G
PG
Shcherbakov
A V
AV
Perevozchikova
N A
NA
Gusev
A A
AA
D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004274:DNA, Recombinant / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D008970:Molecular Weight; D010957:Plasmids; D012313:RNA / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*
D004274:DNA, Recombinant; D012313:RNA; D012822:Silicon Dioxide; D004247:DNA