Brain substrates activated by electroacupuncture (EA) of different frequencies (II): Role of Fos/Jun proteins in EA-induced transcription of preproenkephalin and preprodynorphin genes.
Antisense oligodeoxynucleotides (ODNs) of c-fos and/or c-jun were used in this study to investigate the role of Fos and Jun proteins in electroacupuncture (EA)-induced transcription of the opioid genes, preproenkephalin (PPE), preprodynorphin (PPD) and proopiomelanocortin (POMC). As the results showed, EA-induced Fos and fun expression was blocked efficiently and specifically by e-fos and c-jun antisense ODNs, respectively. This treatment significantly prevented EA-induced PPD, but not PPE, mRNA expression. These results suggest that Fos and Jun proteins are involved in PPD rather than PPE gene transcription activated by EA stimulation.
Guo
H F
HF
Neuroscience Research Center, Beijing Medical University, PR China. huifu@cshl.org
Tian
J
J
Wang
X
X
Fang
Y
Y
Hou
Y
Y
Han
J
J
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004399:Dynorphins / Q000235:genetics*; D015671:Electroacupuncture; D004745:Enkephalins / Q000235:genetics*; D005260:Female; D017403:In Situ Hybridization; D011498:Protein Precursors / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic / Q000235:genetics*
D004745:Enkephalins; D011498:Protein Precursors; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; C036448:pre-prodynorphin; D004399:Dynorphins; C033660:preproenkephalin