TNF-alpha, IL-1 alpha, IL-6 and ICAM-1 expression in human keratinocytes stimulated in vitro with Escherichia coli heat-shock proteins.
Bacterial heat-shock proteins (HSPs) from Escherichia coli (GroES, GroEL and DnaK) were studied for their ability to induce by themselves the expression and release of tumour necrosis factor-alpha (TNF-alpha), interleukin-1 alpha (IL-1 alpha), interleukin-6 (IL-6) and intercellular adhesion molecule-1 (ICAM-1) by cultured human keratinocytes. The surface expression of ICAM-1 was also investigated. In the supernatants of untreated cells none or a minimal amount of these molecules was found. After 48 h of stimulation with GroEL significant amounts of TNF-alpha, IL-1 alpha, IL-6 and soluble ICAM-1 were detected, reaching maximum concentrations at 1 microgram ml-1. The same effect was elicited by DnaK but to a lesser extent. Treatment of keratinocytes with GroEL and DnaK also increased TNF-alpha, IL-1 alpha, IL-6 and ICAM-1 mRNA levels. GroES showed significant activity only on the expression and release of IL-6. GroEL and DnaK were also able to up-regulate the surface expression of ICAM-1 on keratinocytes. The effects on ICAM-1 expression seemed to be directly due to HSPs and not mediated via cytokines. Furthermore, these effects were due to the properties of HSPs because they were inhibited by specific monoclonal antibodies. These findings support the potential role of HSPs in modulating cell interactions during immunological and inflammatory responses in the skin.
Marcatili
Antonella
A
Istituto di Microbiologia, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
de I'Ero
Gabriella Cipollaro
GC
Istituto di Microbiologia, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
Galdiero
Marilena
M
Istituto di Microbiologia, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
Folgore
Antonio
A
Istituto di Microbiologia, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
Petrillo
Giulio
G
Istituto di Clinica Dermosifilopatica, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D018835:Chaperonin 10 / Q000494:pharmacology; D018834:Chaperonin 60 / Q000494:pharmacology; D004926:Escherichia coli / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000209:etiology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D015854:Up-Regulation
D018835:Chaperonin 10; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1