title: Spontaneous establishment of an Epstein-Barr virus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient.
abstract: An Epstein-Barr virus (EBV)-infected fibroblast line, designated DSEK, was spontaneously established from synovial tissue of a patient with rheumatoid arthritis (RA). DSEK cells expressed EBV nuclear antigens EBNA-1 and EBNA-2 and latent membrane protein LMP-1. Cell surface markers of DSEK cells were similar to those of EBV-negative fibroblast clones derived from synoviocytes and were negative for lymphocyte and macrophage markers. DSEK cells expressed CD44, CD58, and HLA-DR antigens and spontaneously produced interleukin-10 basic fibroblast growth factor and transforming growth factor beta1. These results indicate that rheumatoid synoviocytes can be a target for EBV infection and suggest that EBV may play a role in the pathogenesis of RA.
authors.affiliation: Department of Internal Medicine, Saitama Medical Center, Kawagoe, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Koide
authors.forename: K
authors.initials: K
authors.lastname: Takada
authors.forename: M
authors.initials: M
authors.lastname: Sugiura
authors.forename: H
authors.initials: H
authors.lastname: Sekine
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
authors.forename: S
authors.initials: S
authors.lastname: Uchida
authors.forename: T
authors.initials: T
authors.lastname: Abe
mesh_terms: D001172:Arthritis, Rheumatoid / Q000473:pathology / Q000821:virology*; D015415:Biomarkers; D002455:Cell Division; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000821:virology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D013582:Synovial Fluid
chemical_list: D015415:Biomarkers; D016207:Cytokines