Beneficial effects of fluosol-polyethylene glycol cardioplegia on cold, preserved rabbit heart.
BACKGROUND
Because of its high oxygen-carrying capacity, especially at low temperatures, fluosol may enhance heart preservation.


METHODS
Hearts of male New Zealand white rabbits (1.5-2.0 kg) were excised and flushed through the aorta with 0 degree C St. Thomas' Hospital solution, fluosol, or polyethylene glycol or fluosol-polyethylene glycol cardioplegic solution. Hearts were then stored for 12 hours at 0 degree C and reperfused with Krebs-Henseleit buffer at 36.5 degrees C for 60 minutes using a Langendorff system.


RESULTS
Myocardial contractile function was significantly greater in the fluosol-polyethylene glycol cardioplegia-preserved group (p < 0.01) and polyethylene glycol-cardioplegia preserved group (p < 0.05) than in the St. Thomas' Hospital solution-preserved group. The myocardial high-energy phosphate content was significantly higher in the fluosol-polyethylene glycol-cardioplegia-preserved group (p < 0.01), with reduced release of lactate dehydrogenase (p < 0.01) in comparison with the St. Thomas' Hospital solution-preserved group.


CONCLUSIONS
The addition of fluosol and polyethylene glycol to the cardioplegic solution may enhance long-term cold heart preservation.
Bhayana
J N
JN
Department of Surgery, State University of New York at Buffalo, USA.
Tan
Z T
ZT
Bergsland
J
J
Balu
D
D
Singh
J K
JK
Hoover
E L
EL
D000818:Animals; D001802:Blood Substitutes; D002314:Cardioplegic Solutions; D015925:Cryopreservation; D005466:Fluorocarbons; D006321:Heart; D006439:Hemodynamics; D008297:Male; D009206:Myocardium / Q000166:cytology; D009926:Organ Preservation; D011092:Polyethylene Glycols; D011817:Rabbits; D016277:Ventricular Function, Left
D001802:Blood Substitutes; D002314:Cardioplegic Solutions; D005466:Fluorocarbons; D011092:Polyethylene Glycols; C050455:fluosol