Replaceable polymers in DNA sequencing by capillary electrophoresis.
Much progress has been made in the development of replaceable sieving polymers and capillary coatings for high-performance DNA sequencing by capillary electrophoresis. Several studies of parameters that affect resolution, read length and reproducibility have begun to reveal the physical mechanisms acting on single-stranded DNA during electrophoresis through semidilute polymer solutions. Recently developed electro-osmosis-inhibiting matrix polymers have simplified the process of coating capillaries, facilitating the automation of high-throughput parallel systems for large-scale sequencing.
Quesada
M A
MA
Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA. quesada@genome1.bio.bnl.gov
D001709:Biotechnology; D019075:Electrophoresis, Capillary / Q000379:methods*; D005069:Evaluation Studies as Topic; D008956:Models, Chemical; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012996:Solutions
D011108:Polymers; D012996:Solutions