title: [Detection of mycobacterial DNA with polymerase chain reaction in eye discharge and gastric juices in a case of scleritis].
abstract: We report a case of mycobacterial scleritis in which prompt diagnosis was made by the detection of mycobacterial DNA with polymerase chain reaction (PCR) in eye discharge and gastric juices, when conventional tests were negative. A 77-year-old woman who had a past history of pulmonary tuberculosis visited the outpatient clinic of Tokai University Hospital complaining of pain in her right eye. She was diagnosed as having scleritis and uveitis. There were no indications of active tuberculosis. We examined the gastric juices, sputum, and eye discharge by microscopy, culture, and PCR for detection of mycobacterium. The results of microscopy and culture were negative, but with PCR we detected atypical mycobacterium in eye discharge and gastric juices. After oral treatment with antituberculosis agents, the patient's eye symptoms disappeared. Detecting mycobacterial DNA with PCR could be useful for early diagnosis of mycobacterial scleritis, so that treatment with antituberculosis agents could be started.
authors.affiliation: Department of Ophthalmology, Tokai University School of Medicine, Kanagawa-ken, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tanemoto
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
authors.forename: K
authors.initials: K
authors.lastname: Kigasawa
authors.forename: H
authors.initials: H
authors.lastname: Obazawa
authors.forename: H
authors.initials: H
authors.lastname: Fusegawa
authors.forename: H
authors.initials: H
authors.lastname: Miyachi
authors.forename: Y
authors.initials: Y
authors.lastname: Ando
mesh_terms: D000368:Aged; D005122:Exudates and Transudates / Q000382:microbiology; D005123:Eye / Q000378:metabolism; D005260:Female; D005750:Gastric Juice / Q000382:microbiology*; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015423:Scleritis / Q000382:microbiology*; D014392:Tuberculosis, Ocular / Q000382:microbiology*