title: Quantitative nucleic acids footprinting: thermodynamic and kinetic approaches.
abstract: Quantitative footprinting techniques allow a detailed analysis of the thermodynamic forces that characterize nucleic acid-ligand interactions and ligand-induced changes in nucleic acid structure by separately resolving the intrinsic and cooperative Gibbs free energy changes describing the reactions being investigated. A new implementation of the quantitative footprinting technique is the application of stopped-flow techniques to the study of kinetic reactions.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
authors.forename: V
authors.initials: V
authors.lastname: Petri
authors.forename: M
authors.initials: M
authors.lastname: Brenowitz
mesh_terms: D001665:Binding Sites; D001709:Biotechnology; D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting / Q000379:methods*; D003850:Deoxyribonuclease I; D007700:Kinetics; D008024:Ligands; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D008024:Ligands; D012313:RNA; D004247:DNA; D003850:Deoxyribonuclease I