Structure and functional significance of the carbohydrates of the LH/CG receptor.
The LH/CG appears to contain 1-4 bi- or multi-antennary complex-type N-linked oligosaccharide side chains, which appear to locate apart from the hormone-binding regions. The exact sites to which the N-linked chains are attached remain to be delineated. The carbohydrates of the mature membrane-inserted receptor do not contribute to either specific high-affinity ligand-binding or signal transduction of the receptor. Thus, the polypeptide core of the receptor is responsible for both high affinity binding and dictating the hormone specificity. Moreover, the deglycosylated receptor, once inserted to the plasma membrane in a functionally mature form, retains its functional conformation or permits the conformational change that is required for coupling of the receptor to effector enzymes. Addition of oligosaccharides to the nascent LH/CG receptor but not their subsequent conversion to complex-type ones appears to be required for acquiring the hormone-binding conformation. On the other hand, neither addition of oligosaccharides to the nascent receptor, nor their further maturation are needed for the transport of the receptor to the plasma membrane. Thus, one function of the N-linked oligosaccharides in the LH/CG receptor appears to be to direct the proper folding of the receptor.
Rajaniemi
H J
HJ
Biocenter Oulu, University of Oulu, Finland.
Petäjä-Repo
U E
UE
Pietilä
E M
EM
D000818:Animals; D002241:Carbohydrates / Q000737:chemistry / Q000502:physiology; D006801:Humans; D017510:Protein Folding; D011974:Receptors, LH / Q000737:chemistry* / Q000502:physiology*; D015398:Signal Transduction; D013329:Structure-Activity Relationship
D002241:Carbohydrates; D011974:Receptors, LH