Assays for poliovirus polymerase, 3D(Pol), and authentic RNA replication in HeLa S10 extracts.
Barton
D J
DJ
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville 32610, USA.
Morasco
B J
BJ
Flanegan
J B
JB
D001483:Base Sequence; D002846:Chromatography, Affinity / Q000379:methods; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004926:Escherichia coli; D006367:HeLa Cells; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D010448:Peptide Initiation Factors / Q000302:isolation & purification* / Q000378:metabolism; D017955:Poliovirus / Q000201:enzymology* / Q000235:genetics; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014158:Transcription, Genetic; D014779:Virus Replication
D007202:Indicators and Reagents; D010448:Peptide Initiation Factors; D012367:RNA, Viral; D011994:Recombinant Proteins; C090443:polymerase 3Dpol, poliovirus; D012324:RNA-Dependent RNA Polymerase; D012321:DNA-Directed RNA Polymerases