High-speed DNA sequencing in ultrathin slab gels.
There have been many recent theoretical and technical advances in the sequencing of DNA in ultrathin slab gels. These include recent experimental progress in four areas: DNA polymerases; DNA template preparation; the separation and detection of DNA bands; and base-calling algorithms. The Zimm-Lumpkin theory of electrophoresis provides a new and improved framework for understanding the operation of sequencing gels.
Yager
T D
TD
Visible Genetics Inc, 700 Bay Street, Suite 1000, Box 333, Toronto M5G 1Z6, Ontario, Canada.
Dunn
J M
JM
Stevens
J K
JK
D000465:Algorithms; D001709:Biotechnology; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018076:DNA, Complementary / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005069:Evaluation Studies as Topic; D015870:Gene Expression; D016678:Genome; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000639:trends
D018076:DNA, Complementary; D004247:DNA; D004259:DNA-Directed DNA Polymerase