Regional differences in NaCl-induced increase of the potency of bicuculline to displace [3H]muscimol binding.
It has been shown that the potency of bicuculline to displace [3H]muscimol binding to crude brain membranes can be enhanced markedly by different anions. This study shows that although bicuculline alone was a more potent displacer of [3H]muscimol binding in cortical than in cerebellar membranes, the NaCl (250 mM)-induced leftward shift of the bicuculline inhibition curve of [3H]muscimol binding was considerably higher in cerebellum than in cortex. The some concentration of NaCl failed to affect either the affinity or the density of cortical and cerebellar [3H]muscimol binding sites. The results suggest that sodium chloride is able to reveal regional differences in bicuculline potency.
Bujas
M
M
Ruder Bosković Institute, Laboratory for Molecular Neuropharmacology, Zagreb, Croatia.
Tvrdeić
A
A
Pericić
D
D
D000704:Analysis of Variance; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology*; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D004357:Drug Synergism; D018756:GABA Antagonists / Q000494:pharmacology*; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D009118:Muscimol / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000494:pharmacology*; D014316:Tritium
D018756:GABA Antagonists; D014316:Tritium; D009118:Muscimol; D012965:Sodium Chloride; D001640:Bicuculline