Biochemical characterization of FMDV A10 and A22 subtypes by PAGE and IEF.
Both polyacrylamide gel electrophoresis (PAGE) and iso-electric focusing (IEF) have been standardized using the sucrose density gradient purified 146S particles of FMD virus subtypes A10 and A22. Differences in the molecular weights of structural proteins (VP1, VP2 and VP3 of two subtypes (A10 and A22) of FMDV have been revealed in PAGE but no appreciable differences in the pI of VP1, VP2 and VP3 is found in IEF.
Nandi
S
S
FMD Research Centre, Indian Veterinary Research Institute, Hebbal, Bangalore, India.
Suryanarayana
V V
VV
Banumathi
N
N
Sen
A K
AK
D005537:Aphthovirus / Q000737:chemistry*; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008970:Molecular Weight; D015678:Viral Structural Proteins / Q000032:analysis* / Q000302:isolation & purification*
D015678:Viral Structural Proteins