C/EBP alpha is a major activator for the transcription of rat Cu/Zn superoxide dismutase gene in liver cell.
The rat Cu/Zn superoxide dismutase (SOD1) is expressed in all tissues. Sequence analysis of the SOD1 promoter region showed that none of the cis-elements of hepatocyte-specific nuclear factors (HNF) were observed. The cis-element of C/EBP alpha in the proximal region of the SOD1 promoter and the high level of C/EBP alpha in the liver tissue led us to focus on the transcriptional regulation of the SOD1 gene by C/EBP alpha. Cotransfection assays with the plasmid expressing transcription factor C/EBP alpha showed that C/EBP alpha transactivated SOD1 gene by 27 fold. The marked transactivation and direct binding of C/EBP alpha to the SOD1 promoter were confirmed by deletion analyses and mobility shift assays. These results suggested that C/EBP alpha plays a major role in the tissue distribution of SOD1.
Kim
Y H
YH
Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University, South Korea.
Yoo
H Y
HY
Chang
M S
MS
Jung
G
G
Rho
H M
HM
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D004247:DNA; D004268:DNA-Binding Proteins / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology*; D011401:Promoter Regions, Genetic; D051381:Rats; D013482:Superoxide Dismutase / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D004247:DNA; D013482:Superoxide Dismutase