title: Aspects of protein bound copper in sheep plasma and its release in vitro especially after treatment with ammonium tetrathiomolybdate.
abstract: The distribution of protein-bound copper in sheep plasma between a globulin and an albumin fraction, as well as copper release in vitro by means of various agents, either from the above-mentioned fractions or from those formed after ammonium tetrathiomolybdate (TTM) treatment, were studied. Copper bound in both plasma globulin and albumin (CuGA) is 95.1% of the total plasma copper (CuT); copper release from the globulin fraction by means of trichloracetic acid (TCA) or cyanides (CN-) is relatively low, 17.1% and 25.8% respectively, while there is not any copper release after TTM treatment; however, the copper release from the albumin fraction by means of TCA and CN- is relatively high, 59.2% and 74.9%, respectively, while the treatment with TTM has the same effect of no copper release as mentioned above for the globulin fraction.
authors.affiliation: Chemistry Laboratory of the Veterinary Department, Aristotle University of Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Ayiannidis
authors.forename: S
authors.initials: S
authors.lastname: Giroussi
authors.forename: A
authors.initials: A
authors.lastname: Voulgaropoulos
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D003300:Copper / Q000097:blood*; D005916:Globulins / Q000378:metabolism; D008982:Molybdenum / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D012709:Serum Albumin / Q000378:metabolism; D012756:Sheep
chemical_list: D001798:Blood Proteins; D005916:Globulins; D012709:Serum Albumin; D003300:Copper; D008982:Molybdenum; C020809:tetrathiomolybdate