title: Nucleotide-sugar binding to ribosomes. Comparison of affinity and capacity of gastric mucosa and liver ribosomes to sugar-nucleotides.
abstract: Highly purified ribosomes from gastric mucosa and liver were isolated and tested for their affinity and capacity toward UDP- monosaccharide binding. Ribosomes from both tissues bind UDP-N-acetylglucosamine nearly at the same level with similar affinity, but binding capacity of gastric ribosomes to UDP-N-acetylgalactosamine was twice higher than in case of liver ribosomes, and with higher affinity than in case of UDP-N-acetylglucosamine. The binding of UDP-N-acetylgalactosamine to mucosa ribosomes requires the whole sugar-nucleotide structure.
authors.affiliation: Department of General and Organic Chemistry, Medical Academy of Białystok.
authors.forename: A
authors.initials: A
authors.lastname: Paszkiewicz-Gadek
authors.forename: A
authors.initials: A
authors.lastname: Gindzieński
authors.forename: H
authors.initials: H
authors.lastname: Porowska
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism*; D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis; D006031:Glycosylation; D008099:Liver / Q000378:metabolism*; D008297:Male; D009711:Nucleotides / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012270:Ribosomes / Q000378:metabolism*; D013552:Swine; D014530:Uridine Diphosphate / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D009711:Nucleotides; D014530:Uridine Diphosphate; D000116:Acetylgalactosamine; D000117:Acetylglucosamine