title: Hepatitis C virus NS5B protein is a membrane-associated phosphoprotein with a predominantly perinuclear localization.
abstract: Hepatitis C virus NS5B protein is an RNA-dependent RNA polymerase. To investigate the properties and function of this protein, we have expressed the NS5B protein in insect and mammalian cells. NS5B was found to be present as fine speckles in the cytoplasm, particularly concentrated in the perinuclear region, suggesting its association with the nuclear membrane, the endoplasmic reticulum, or the Golgi complex. This conclusion was supported by the biochemical demonstration that NS5B was associated with the membranes in the cells. Furthermore, it was shown that NS5B protein is a phosphoprotein. These properties may be related to its function as an RNA polymerase.
authors.affiliation: Institute of Environment and Life Science, The Hallym Academy of Sciences, Hallym University, Chuncheon, Korea.
authors.forename: S B
authors.initials: SB
authors.lastname: Hwang
authors.forename: K J
authors.initials: KJ
authors.lastname: Park
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: Y C
authors.initials: YC
authors.lastname: Sung
authors.forename: M M
authors.initials: MM
authors.lastname: Lai
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology / Q000648:ultrastructure; D016174:Hepacivirus / Q000201:enzymology*; D009685:Nuclear Envelope / Q000201:enzymology / Q000648:ultrastructure; D017924:Nucleopolyhedroviruses; D015388:Organelles / Q000201:enzymology / Q000648:ultrastructure; D010750:Phosphoproteins / Q000032:analysis / Q000096:biosynthesis; D010766:Phosphorylation; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D018411:Spodoptera; D013347:Subcellular Fractions / Q000201:enzymology; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D010750:Phosphoproteins; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase