Laser scanning cytometry in pathology of solid tumors. A review.
OBJECTIVE
To review application of laser scanning cytometry (LSC) to analyze several different parameters of human solid tumors in relation to the cell cycle.


METHODS
Tissue sections of cytology specimens were stained for specific parameters and analyzed by LSC.


RESULTS
Examples of LSC analysis of the expression of ER, Ki-67, cyclin B1, BrdUrd and ploidy are given.


CONCLUSIONS
LSC provides rapid, high-precision measurement of the chosen parameters or constituents of each cell in a population of cells and correlates those measurements with the visual image of the corresponding cell.
Gorczyca
W
W
Department of Pathology, New York Medical College, Valhalla 10595, USA.
Darzynkiewicz
Z
Z
Melamed
M R
MR
D017209:Apoptosis; D001943:Breast Neoplasms / Q000737:chemistry; D002453:Cell Cycle; D002455:Cell Division; D018844:Cyclin-Dependent Kinases / Q000032:analysis; D016213:Cyclins / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D005470:Fluorometry / Q000295:instrumentation*; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation*; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D019394:Ki-67 Antigen / Q000032:analysis; D007834:Lasers; D009363:Neoplasm Proteins / Q000032:analysis*; D009369:Neoplasms / Q000737:chemistry / Q000473:pathology*; D011003:Ploidies; D011956:Receptors, Cell Surface / Q000032:analysis
D016213:Cyclins; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; D018844:Cyclin-Dependent Kinases