title: A novel bend of DNA CIT: changeable bending-center sites of an intrinsic curvature under temperature conditions.
abstract: We found a novel DNA curvature, which has changeable bending-center sites of an intrinsic curvature under temperature conditions (CIT) in the cyanobacterium strain Microcystis aeruginosa K-81. Circular permutation analyses (CPA) for CIT under different temperature conditions (4-50 degrees C) revealed that the changeable bending-center sites are located in the 5'-upstream region (-141 to -184) of the psbA2 gene, encoding the D1 protein homolog for photosynthesis. The nucleotide sequence around the bending center contains several dT (deoxy thymine) tracts, which seem to be a pivotal determinant for CIT.
authors.affiliation: Division of Biotechnology, Ibaraki University, Japan.
authors.forename: G K
authors.initials: GK
authors.lastname: Agrawal
authors.forename: M
authors.initials: M
authors.lastname: Asayama
authors.forename: M
authors.initials: M
authors.lastname: Shirai
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry* / Q000187:drug effects; D004214:Distamycins / Q000494:pharmacology; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D045332:Photosystem II Protein Complex; D010957:Plasmids; D015183:Restriction Mapping; D013696:Temperature
chemical_list: D000998:Antiviral Agents; D004269:DNA, Bacterial; D004214:Distamycins; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C030000:stallimycin