title: Effect of gene therapy with the herpes simplex virus-thymidine kinase gene on hepatic metastasis in murine colon cancer.
abstract: Hepatic metastasis of colon cancer is an important prognostic factor for survival. In this study, we examined the effect of gene therapy using the herpes simplex virus-thymidine kinase (HS-tk) gene with short-course ganciclovir (GCV) treatment for multiple hepatic metastases of murine colon cancer. Colon26 cells transfected with the HS-tk gene were found to be sensitive to GCV in a concentration-dependent way. On the other hand, induction of the HS-tk gene in the cells had no influence on cell growth in vitro. However, multiple hepatic metastases of Colon26 cells transfected with HS-tk gene were significantly suppressed by the GCV treatment. These results thus suggest that HS-tk gene therapy is useful for the treatment of hepatic metastasis in colon cancer.
authors.affiliation: Second Department of Surgery, Nagoya University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: N
authors.initials: N
authors.lastname: Emi
authors.forename: I
authors.initials: I
authors.lastname: Yokoyama
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: H
authors.initials: H
authors.lastname: Takagi
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology / Q000628:therapy*; D005260:Female; D015774:Ganciclovir / Q000627:therapeutic use*; D015316:Genetic Therapy; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000556:secondary* / Q000628:therapy; D051379:Mice; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D013937:Thymidine Kinase; D015774:Ganciclovir