title: The structure and function of a soybean beta-glucan-elicitor-binding protein.
abstract: beta-Glucan elicitor (GE), released from the cell wall of the phytopathogenic fungus Phytophthora megasperma by soybean glucanases, causes defense reactions in soybean. A GE-binding protein (GEBP) was purified from the membrane fraction of soybean root cells, and its cDNA was isolated. Expression of the cDNA clone in tobacco suspension cultured cells and in Escherichia coli conferred GE-binding activity to both. An antibody against the recombinant protein was found to inhibit the GE binding with the soybean cotyledon membrane fraction as well as the resulting accumulation of phytoalexin. Immunolocalization assays indicated that the GEBPs are located in the plasma membrane of root cells. These results suggest that the cDNA encodes a GE receptor and may mediate the signaling of the elicitor.
authors.affiliation: Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Fukuura, Kanazawa-ku, Yokohama, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Umemoto
authors.forename: M
authors.initials: M
authors.lastname: Kakitani
authors.forename: A
authors.initials: A
authors.lastname: Iwamatsu
authors.forename: M
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: N
authors.initials: N
authors.lastname: Yamaoka
authors.forename: I
authors.initials: I
authors.lastname: Ishida
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005936:Glucans / Q000302:isolation & purification / Q000378:metabolism*; D037102:Lectins; D058619:Maltose-Binding Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins; D010838:Phytophthora; D018517:Plant Roots / Q000737:chemistry; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D011993:Recombinant Fusion Proteins; D017421:Sequence Analysis; D015398:Signal Transduction; D013025:Glycine max / Q000737:chemistry / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D029968:Escherichia coli Proteins; D005936:Glucans; D037102:Lectins; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; C037937:glucan-binding proteins; C461599:maltose transport system, E coli