Sensitive gas chromatographic method for determination of mercapturic acids in human urine.
A method was developed for sensitive determination of the specific benzene metabolite S-phenylmercapturic acid and the corresponding toluene metabolite S-benzylmercapturic acid in human urine for non-occupational and occupational exposure. The sample preparation procedure consists of liquid extraction of urine samples followed by precolumn derivatization and a clean-up by normal-phase HPLC. Determination of analytes occurs by gas chromatography with electron-capture detection. With this highly sensitive method (detection limits 60 and 65 ng/l, respectively) urinary S-phenylmercapturic and S-benzylmercapturic acid concentrations for non-occupationally exposed persons (e.g. non-smokers) can be measured precisely in one chromatographic run. Validation of the method occurred by comparison with a HPLC method we have published recently.
Einig
T
T
Medizinisches Institut für Umwelthygiene, Abteilung Biochemic, Düsseldorf, Germany.
Dunemann
L
L
Dehnen
W
W
D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods*; D004781:Environmental Exposure; D006801:Humans; D016273:Occupational Exposure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012907:Smoking
C053003:S-phenyl-N-acetylcysteine; D000111:Acetylcysteine; C029319:S-benzyl-N-acetyl-L-cysteine