Multichannel microchip electrospray mass spectrometry.
Microfabricated multiple-channel glass chips were successfully interfaced to an electrospray ionization mass spectrometer (ESI-MS). The microchip device was fabricated by standard photolithographic, wet chemical etching, and thermal bonding procedures. A high voltage was applied individually from each buffer reservoir for spraying sample sequentially from each channel. With the sampling orifice of the MS grounded, it was found that a liquid flow of 100-200 nL/min was necessary to maintain a stable electrospray. The detection limit of the microchip MS experiment for myoglobin was found to be lower than 6 x 10(-8) M. Samples in 75% methanol were successfully analyzed with good sensitivity, as were aqueous samples. The parallel mutliple-channel microchip system allowed ESI-MS analysis of different samples of standard peptides and proteins in one chip.
Xue
Q
Q
Barnett Institute, Northeastern University, Boston, Massachusetts 02115, USA.
Foret
F
F
Dunayevskiy
Y M
YM
Zavracky
P M
PM
McGruer
N E
NE
Karger
B L
BL
D013058:Mass Spectrometry / Q000295:instrumentation*; D008838:Microcomputers; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis
D010455:Peptides; D011506:Proteins