title: Solubility diagram of the Rhodobacter sphaeroides reaction center as a function of PEG concentration.
abstract: In order to quantify the effect of polyethylene glycol 4000 (PEG) on the solubility of an integral membrane protein, we have crystallized the photochemical reaction center from Rhodobacter sphaeroides Y by batch method on a large range of PEG. The measurement of the solubility diagram display a semi-logarithmic dependence of solubility versus PEG concentration. Comparison of our results with previously published ones [Odahara, T., Ataka, M. and Katsura, M. (1994) Acta Cryst. D50, 639-642] suggests a notable effect of additional 1,2,3-heptane-triol and/or temperature on photochemical reaction center solubility.
authors.affiliation: Laboratoire d'Enzymologie et de Biochimie Structurales, CNRS, Gif-sur-Yvette, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Gaucher
authors.forename: M
authors.initials: M
authors.lastname: Riès-Kautt
authors.forename: F
authors.initials: F
authors.lastname: Reiss-Husson
authors.forename: A
authors.initials: A
authors.lastname: Ducruix
mesh_terms: D003460:Crystallization; D007700:Kinetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D012995:Solubility
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D011092:Polyethylene Glycols