Sequencing and mapping the Arabidopsis genome: a weed model for real crops.
Arabidopsis is a crucifer weed with a small genome of about 120 Mbp which has been chosen as a model species for plant molecular genetics. Four years ago, a consortium of nine French laboratories, including ours, initiated a project aimed at mapping the transcribed regions of the genome. The strategy employed was to systematically and randomly sequence cDNA clones isolated from libraries made from different tissues and organs of plants grown under various physiological conditions. The consortium released about 7,000 expressed sequenced tags (ESTs) in the dbEST database corresponding to approximately 3,500 unique genes. In the next phase of the programme, a YAC library with average inserts of 500 kbp has been prepared. We have now started to use the EST information to map the cDNA clones on these YACs. The most recent aspect of Arabidopsis sequencing is the ESSA (European Scientists Sequencing Arabidopsis) project, in which the aim is to describe 2.5 Mbp by the end of 1996. Genomic sequencing has revealed a very high gene density. Comparison of present genomic sequencing results with the EST data suggests that up to half of the genes might already be tagged with an EST. In collaboration with Carlos Quiros' group in Davis we have also analysed the conservation of a 30 kbp locus (Em 1, a late embryogenesis abundant protein gene) on chromosome 3 between Arabidopsis and several Brassica species. Progress on these various aspects will be reviewed. We shall also present some sequence comparisons between Arabidopsis and rice ESTs. These results suggest that it should be possible in the very near future to map a pool of common genes onto many different plant genomes. This should provide a common framework to integrate maps from different species and facilitate mapbased cloning of genes of agronomical importance.
Delseny
M
M
Physiologie et Biologie Moléculaire des Plantes, CNRS Unite 565, University of Perpignan, France.
Raynal
M
M
Laudié
M
M
Varoquaux
F
F
Comella
P
P
Wu
H J
HJ
Cooke
R
R
Grellet
F
F
D017360:Arabidopsis / Q000235:genetics*; D002874:Chromosome Mapping; D018076:DNA, Complementary; D015870:Gene Expression; D017343:Genes, Plant; D008957:Models, Genetic; D012275:Oryza / Q000235:genetics; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary