Characterization of binding interactions by isothermal titration calorimetry.
Isothermal titration calorimetry is a high-accuracy method for measuring binding affinities. Titration calorimetry is a universal method that has broad impact throughout biotechnology. In recent years, microcalorimeters that are capable of characterizing binding interactions of biological macromolecules have become commercially available. Results from these studies are providing new insight into the molecular nature of macromolecular interactions.
Doyle
ML
Macromolecular Sciences Department, UE-0447-B SmithKline Beecham Pharmaceuticals 709 Swedeland Road King of Prussia PA 19406-0939 USA Michael_L_Doyle@sbphrd.com