title: Identification of rat serum alkaline phosphatase isoenzyme by means of wheat germ agglutinin.
abstract: Wheat germ agglutinin (WGA) precipitates bone type serum alkaline phosphatase (sALP) isoenzyme specifically. The precipitates are composed of the macromolecules of WGA and "bone type sALP" (WGA-ALP complex). In order to use bone type sALP as a marker in polyacrylamide gel electrophoresis (PAGE), a method to separate "bone type sALP" from the "WGA-ALP complex" was established by using N-acetyl-D-glucosamine (GlcNAc)-Sepharose 6E column chromatography. It was concluded that this method is useful for clinical examination in the rat.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: N
authors.initials: N
authors.lastname: Niwa
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: H
authors.initials: H
authors.lastname: Tsuge
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001842:Bone and Bones / Q000201:enzymology; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000097:blood*; D008297:Male; D051381:Rats; D014909:Wheat Germ Agglutinins
chemical_list: D007527:Isoenzymes; D014909:Wheat Germ Agglutinins; D000469:Alkaline Phosphatase