Cloning and expression analysis of a gene that shows developmental regulation upon tuberization in potato.
Differential screening of a potato leaf cDNA library with cDNA probes made from tuberizing and non-tuberizing Solanum demissum plants led to the identification of a clone that is upregulated in leaves and other tissues upon tuberization. This clone was also shown to have a high level of expression in green tomato fruit, its expression falling off as the fruit turns red. No sucrose or hormonal regulation of the expression of this clone was observed and it did not respond to wounding or heat stress. Clone 32B is 532 bp long and contains an open reading frame encoding a small protein of 98 amino acids. The deduced protein sequence has a putative signal peptide for ER transport and a 10 amino acid domain in the C-terminal region of the protein, both of which are also found in the cotton LEA5, Arabidopsis Di21 and the mungbean Arg2 proteins.
Jackson
S
S
Centro de Investigacion y Desarrollo, C.S.I.C., Barcelona, Spain.
Gascón
J
J
Carrera
E
E
Monte
E
E
Prat
S
S
D000595:Amino Acid Sequence; D015342:DNA Probes; D018076:DNA, Complementary; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D008969:Molecular Sequence Data; D018515:Plant Leaves; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry*; D018517:Plant Roots; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000235:genetics / Q000254:growth & development*
D015342:DNA Probes; D018076:DNA, Complementary; D010940:Plant Proteins