Molecular cloning of the nemA gene encoding N-ethylmaleimide reductase from Escherichia coli.
Using the gene mapping membrane technique, we identified a gene (nemA) that encodes N-ethylmaleimide reductase in Escherichia coli. The open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39,514 Da. The deduced amino acid sequence showed a high degree of homology (87% identical) with the pentaerythritol tetranitrate reductase of Enterobacter cloacae and the morphinone reductase of Pseudomonas putida (52% identical).
Miura
K
K
Department of Pharmaceutical Sciences, Tohoku University Hospital, Aoba-ku, Sendai, Japan.
Tomioka
Y
Y
Suzuki
H
H
Yonezawa
M
M
Hishinuma
T
T
Mizugaki
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005033:Ethylmaleimide / Q000378:metabolism*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*
D010088:Oxidoreductases; D005033:Ethylmaleimide