Interaction of kunjin virus with octyl-D-glucoside extracted Vero cell plasma membrane.
Initial experiments using whole cells have shown that there were specific and saturable interactions between kunjin (KUN) virus and receptor molecules on the Vero cell surfaces. Solubilisation of Vero cell plasma membranes with octyl-D-glucoside (OG) yielded an extract which also interacted specifically with KUN virus. This was proven using electron microscopy. When the virus-OG-extract complex was exposed onto Vero cell monolayers, no KUN virus was observed to enter into the whole cells. This would imply that there was virus-receptor interaction with the OG-extract leaving no free virus to attach to the whole cells. The attachment kinetics of KUN virus was studied further using the Scatchard analysis which indicated the involvement of more than one interactive macromolecule in the attachment event.
Sankaran
D
D
Department of Microbiology, National University of Singapore.
Lau
L C
LC
Ng
M L
ML
D000818:Animals; D002457:Cell Extracts; D002462:Cell Membrane / Q000821:virology*; D002522:Chlorocebus aethiops; D003902:Detergents / Q000737:chemistry*; D018349:Encephalitis Viruses, Japanese / Q000378:metabolism* / Q000648:ultrastructure; D005960:Glucosides / Q000737:chemistry*; D007700:Kinetics; D011991:Receptors, Virus / Q000378:metabolism*; D014709:Vero Cells
D002457:Cell Extracts; D003902:Detergents; D005960:Glucosides; D011991:Receptors, Virus; C018619:octyl-beta-D-glucoside