High-throughput microsatellite analysis using fluorescent dUTPs for high-resolution genetic mapping of the mouse genome.
The use of fluorescent end-labeled primers has proved successful for rapid, semiautomated genotyping of microsatellite loci. However, custom synthesis is expensive and costs can be prohibitive when a wide range of markers is to be analyzed for only a few genotypings. This particularly applies to high-resolution genetic mapping in the mouse either in the construction of global maps or in the production of local high-resolution genetic maps for positional cloning. We demonstrate here the use of fluorescent dUTPs for cost-effective, high-throughput microsatellite genotyping in the mouse. This alternative to the use of fluorescent end-labeled primers for semiautomated genotyping is potentially applicable to the construction of linkage maps in other species.
Rhodes
M
M
MRC Human Genome Mapping Project Resource Centre, Hinxton, Cambridge, UK.
Dearlove
A
A
Straw
R
R
Fernando
S
S
Evans
A
A
Greener
M
M
Lacey
T
T
Kelly
M
M
Gibson
K
K
Brown
S D
SD
Mundy
C
C
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; D005819:Genetic Markers; D005838:Genotype; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; D005819:Genetic Markers