Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments.
Aatsinki
J T
JT
Biocenter Oulu, University of Oulu, Finland.
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers; D018076:DNA, Complementary; D004926:Escherichia coli; D007202:Indicators and Reagents; D008297:Male; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D013737:Testis; D013824:Thermus / Q000201:enzymology; D016963:Thermus thermophilus / Q000201:enzymology
D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary; D007202:Indicators and Reagents; D012194:RNA-Directed DNA Polymerase