title: Lack of chondroitin sulphate epitope in the proliferating zone of the growth plate of chicken tibia.
abstract: Monoclonal antibodies specific to chondroitin sulphate (CS-56) and keratan sulphate (AH12) were used to localize proteoglycans in the proximal tibial articular cartilage and growth plate of broiler chickens. There was no CS-56 labelling in the proliferative zone of the growth plate. In contrast, intense labelling with this antibody was observed in the transitional and hypertrophic zones of the growth plate and the articular cartilage. This was confirmed by extracting chondroitin sulphate fractions from different zones of the growth plate and articular cartilage, and examining their antigenicities to CS-56 by ELISA inhibition assay. It was suggested that the maturation of chondrocytes in the growth plate is related to the production of chondroitin sulphate with CS-56 epitope, which may be a prerequisite for normal endochondral bone formation in the chicken tibia. The role of chondroitin sulphate recognized by CS-56 in the articular cartilage is unknown.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: J S
authors.initials: JS
authors.lastname: Sim
authors.forename: S
authors.initials: S
authors.lastname: Imai
authors.forename: T
authors.initials: T
authors.lastname: Koga
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D002809:Chondroitin Sulfates / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006132:Growth Plate / Q000737:chemistry*; D007124:Immunoenzyme Techniques; D013977:Tibia / Q000737:chemistry*; D014574:Uronic Acids / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D014574:Uronic Acids; D002809:Chondroitin Sulfates