Effects of methionine and Cu2+ on the expression of tyrosinase activity in Streptomyces castaneoglobisporus.
Streptomyces castaneoglobisporus HUT6202 expresses an enzyme, tyrosinase, responsible for the production of melanin-like pigments. The present study revealed that the tyrosinase synthesis by the microorganism was induced about 80-fold, when young cells cultured for 6 h were incubated with methionine (Met) to the mid-log phase of growth, in comparison to without this amino acid. The Met-induced tyrosinase synthesis was inhibited by the addition of rifampicin and chloramphenicol, suggesting that transcriptional and translational events are necessary for the induction. We found that the addition of Cu2+ to the culture medium brings forward the period of expression of Met-induced tyrosinase activity.
Ikeda
K
K
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine.
Masujima
T
T
Sugiyama
M
M
D001483:Base Sequence; D003300:Copper / Q000494:pharmacology*; D004790:Enzyme Induction; D008715:Methionine / Q000494:pharmacology*; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis / Q000187:drug effects; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology*; D014158:Transcription, Genetic / Q000187:drug effects
D003300:Copper; D008715:Methionine; D014442:Monophenol Monooxygenase