title: Influence of simulated bed rest and intermittent weight bearing on single skeletal muscle fiber function in aged rats.
abstract: OBJECTIVE
  To characterize specific musculoskeletal contractile property changes that occur during inactivity and intermittent weight bearing in aged muscle.
  
  
  METHODS
  Randomized control trial.
  
  
  METHODS
  A controlled laboratory environment.
  
  
  METHODS
  Fifteen aged rats were randomly assigned to control (CON), hindlimb unweighted (HU), and hindlimb unweighted with intermittent weight bearing (HU-IWB) groups.
  
  
  METHODS
  The HU and HU-IWB rats were suspended for 1 week. The HU-IWB animals were unsuspended four times daily allowing 15 minutes of weight-bearing.
  
  
  METHODS
  Muscle weights, muscle fiber diameter, peak absolute force, peak specific tension (P0), and maximal shortening velocity (V0).
  
  
  RESULTS
  In comparison to CON animals, the soleus (SOL) wet weight was significantly (p < or = .05) reduced by 19% and 6% in HU and HU-IWB animals, respectively. SOL single fiber analysis showed no difference in fiber diameter between the three groups. However, peak absolute force and P0 of SOL type I fibers were significantly (p < or = .05) reduced in the HU group compared to CON values. V0 of SOL fibers increased with HU. In comparison to CON animals, the gastrocnemius (GAS) wet weight was significantly reduced by 9% and 8% in HU and HU-IWB animals, respectively.
  
  
  CONCLUSIONS
  Inactivity significantly altered the contractile properties of single fibers isolated from aged mammalian SOL skeletal muscle. Furthermore, minimal weight bearing attenuated these detrimental effects induced by inactivity in the SOL. However, this weight-bearing protocol did not attenuate the inactivity-induced alterations in aged mammalian GAS skeletal muscle.
authors.affiliation: Master of Physical Therapy Program, College of St. Catherine, Minneapolis, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Alley
authors.forename: L V
authors.initials: LV
authors.lastname: Thompson
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001510:Bed Rest; D001835:Body Weight; D018482:Muscle, Skeletal / Q000502:physiology*; D009929:Organ Size; D051381:Rats; D016474:Weight-Bearing