title: An alpha-E-catenin gene trap mutation defines its function in preimplantation development.
abstract: Catenins are proteins associated with the cytoplasmic domain of cadherins, a family of transmembrane cell adhesion molecules. The cadherin-catenin adhesion system is involved in morphogenesis during development and in the maintenance of the integrity of different tissue types. Using a gene trap strategy, we have isolated a mouse mutation for the gene encoding the alpha-E-catenin. This form of the alpha-catenin appears frequently coexpressed with E-cadherin in epithelial cell types. The mutation obtained eliminates the carboxyl-terminal third of the protein but nevertheless provokes a complete loss-of-function phenotype. Homozygous mutants show disruption of the trophoblast epithelium (the first differentiated embryonic tissue), and development is consequently blocked at the blastocyst stage. This phenotype parallels the defects observed in E-cadherin mutant embryos. Our results show the requirement of the alpha-E-catenin carboxy terminus for its function and represent evidence of the role of the alpha-E-catenin in vivo, identifying this molecule as the natural partner of the E-cadherin in trophoblast epithelium.
authors.affiliation: Abteilung Molekulare Zellbiologie, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Torres
authors.forename: A
authors.initials: A
authors.lastname: Stoykova
authors.forename: O
authors.initials: O
authors.lastname: Huber
authors.forename: K
authors.initials: K
authors.lastname: Chowdhury
authors.forename: P
authors.initials: P
authors.lastname: Bonaldo
authors.forename: A
authors.initials: A
authors.lastname: Mansouri
authors.forename: S
authors.initials: S
authors.lastname: Butz
authors.forename: R
authors.initials: R
authors.lastname: Kemler
authors.forename: P
authors.initials: P
authors.lastname: Gruss
mesh_terms: D000818:Animals; D001483:Base Sequence; D001755:Blastocyst; D015820:Cadherins / Q000378:metabolism*; D002478:Cells, Cultured; D002678:Chimera; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005314:Embryonic and Fetal Development; D004848:Epithelium; D005796:Genes / Q000235:genetics; D005838:Genotype; D007763:Lac Operon / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D017384:Sequence Deletion; D014327:Trophoblasts; D051178:alpha Catenin
chemical_list: D015820:Cadherins; C495272:Ctnna1 protein, mouse; D003598:Cytoskeletal Proteins; D051178:alpha Catenin