title: A transcriptional activation domain of ATMYB2, a drought-inducible Arabidopsis Myb-related protein.
abstract: Trans-activation activity of ATMYB2, a drought-inducible Myb-related protein in Arabidopsis thaliana, was analyzed using a transient assay of Arabidopsis leaf protoplasts. ATMYB2 activated the transcription of a reporter gene from the MYB-binding site in a sequence-specific manner. Deletion of the C-terminal region of ATMYB2 reduced the trans-activation of the reporter gene, indicating that the acidic region at the C-terminus of ATMYB2 is required for transcriptional activation. The domain exchange analysis with the yeast GAL4 revealed that the C-terminal acidic region of ATMYB2 contains a sufficient domain for trans-activation. These results indicate that ATMYB2 acts as a transcriptional activator and that the C-terminal acidic region of ATMYB2 can function as a transcriptional activation domain.
authors.affiliation: Biological Resources Division, Japan International Research Center for Agricultural Science (JIRCAS), Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Urao
authors.forename: M
authors.initials: M
authors.lastname: Noji
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.forename: K
authors.initials: K
authors.lastname: Shinozaki
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics*; D029681:Arabidopsis Proteins; D004190:Disasters; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D010940:Plant Proteins / Q000235:genetics*; D011523:Protoplasts; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014867:Water / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C104175:MYB2 protein, Arabidopsis; D010940:Plant Proteins; D015534:Trans-Activators; D014867:Water