Destabilisation of native tertiary structural interactions is linked to helix-induction by 2,2,2-trifluoroethanol in proteins.
The effect of 2,2,2-trifluoroethanol (TFE) on the structure of an all beta-sheet protein, cardiotoxin analogue 111 (CTX III) from the Taiwan cobra (Naja naja atra) is studied. It is found that high concentrations (> 80% v/v) of TFE induced a beta-sheet to alpha-helix structural transition. It is found that in denatured and reduced CTX III (rCTX III) helical conformation is induced even upon addition of low concentrations (> 10% v/v) of TFE. Using three other proteins, namely, ribonuclease A (RNase A), lysozyme and alpha-lactalbumin, it is been observed that helix-induction by TFE is intricately linked to drastic destabilization of native tertiary structural interactions in the proteins.
Sivaraman
T
T
Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
Kumar
T K
TK
Yu
C
C
D002942:Circular Dichroism; D004179:Cobra Cardiotoxin Proteins / Q000737:chemistry; D007768:Lactalbumin / Q000737:chemistry / Q000187:drug effects; D009113:Muramidase / Q000737:chemistry / Q000187:drug effects; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary / Q000187:drug effects; D017434:Protein Structure, Tertiary / Q000187:drug effects*; D011506:Proteins / Q000737:chemistry* / Q000187:drug effects; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000187:drug effects; D014270:Trifluoroethanol / Q000737:chemistry / Q000494:pharmacology*
D004179:Cobra Cardiotoxin Proteins; D011506:Proteins; C082202:cardiotoxin III, Naja naja atra; D014270:Trifluoroethanol; D007768:Lactalbumin; D012259:Ribonuclease, Pancreatic; D009113:Muramidase