Production and gene expression of IL-8-like cytokine GRO/CINC-1 in rat nasal mucosa.
Growth-regulated gene product/cytokine-induced neutrophil chemoattractant (GRO/CINC)-1 is a rat chemokine with structural and functional homology to human IL-8. Chemokines are a family of cytokines whose participation in nasal inflammation in vivo remains to be established. Using ELISA and RT-PCR, we investigated the production and gene expression of GRO/CINC-1 in rat nasal lavage and mucosa in vivo. GRO/CINC-1 in nasal lavage was produced by stimulation of LPS, ConA and IL1-beta. GRO/CINC-1 showed time- and dose-dependent production under all stimulants, but was more slowly induced by IL-1 beta. The steady-peak of the GRO/CINC-1 production remained at 3 h with LPS or ConA exposure, whereas it lasted 4 h or more after IL-1 beta exposure. At the time of peak production of GRO/CINC-1, we found that mRNA for the GRO/CINC-1 was induced in the nasal mucosa. The mRNA of the related inflammatory cytokines TNF-alpha and IFN-gamma were also expressed in nasal mucosa with stimulation of these reagents. Thus, this study revealed that exposure to bacterial endotoxin, mitogenic reagent and also IL-1 beta induced the production and gene expression of the neutrophil chemoattractant GRO/CINC-1 in rat nasal mucosa in vivo. This investigation of the characteristics of IL-8 family in nasal mucosa using rat models has extended the functional concept of cytokines in the inflammatory condition of nasal cavity in humans.
Himi
T
T
Department of Otolaryngology, Sapporo Medical University, School of Medicine, Japan.
Yoshioka
I
I
Kataura
A
A
D000818:Animals; D018925:Chemokines / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009297:Nasal Mucosa / Q000737:chemistry / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
D018925:Chemokines; D017931:DNA Primers; D016209:Interleukin-8; D012333:RNA, Messenger