title: Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5.
abstract: A complementary DNA clone has been isolated that encodes a coxsackievirus and adenovirus receptor (CAR). When transfected with CAR complementary DNA, nonpermissive hamster cells became susceptible to coxsackie B virus attachment and infection. Furthermore, consistent with previous studies demonstrating that adenovirus infection depends on attachment of a viral fiber to the target cell, CAR-transfected hamster cells bound adenovirus in a fiber-dependent fashion and showed a 100-fold increase in susceptibility to virus-mediated gene transfer. Identification of CAR as a receptor for these two unrelated and structurally distinct viral pathogens is important for understanding viral pathogenesis and has implications for therapeutic gene delivery with adenovirus vectors.
authors.affiliation: Division of Infectious Diseases, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Bergelson
authors.forename: J A
authors.initials: JA
authors.lastname: Cunningham
authors.forename: G
authors.initials: G
authors.lastname: Droguett
authors.forename: E A
authors.initials: EA
authors.lastname: Kurt-Jones
authors.forename: A
authors.initials: A
authors.lastname: Krithivas
authors.forename: J S
authors.initials: JS
authors.lastname: Hong
authors.forename: M S
authors.initials: MS
authors.lastname: Horwitz
authors.forename: R L
authors.initials: RL
authors.lastname: Crowell
authors.forename: R W
authors.initials: RW
authors.lastname: Finberg
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D029822:Enterovirus B, Human / Q000378:metabolism* / Q000502:physiology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment; D014162:Transfection; D014779:Virus Replication
chemical_list: C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus