title: Structural and functional conservation of the Caenorhabditis elegans timing gene clk-1.
abstract: Mutations in the Caenorhabditis elegans gene clk-1 affect biological timing and extend longevity. The gene clk-1 was identified, and the cloned gene complemented the clk-1 phenotypes and restored normal longevity. The CLK-1 protein was found to be conserved among eukaryotes, including humans, and structurally similar to the yeast metabolic regulator Cat5p (also called Coq7p). These proteins contain a tandem duplication of a core 82-residue domain. clk-1 complemented the phenotype of cat5/coq7 null mutants, demonstrating that clk-1 and CAT5/COQ7 share biochemical function and that clk-1 acts at the level of cellular physiology.
authors.affiliation: Department of Biology, McGill University, 1205 Avenue Docteur Penfield, Montréal, Québec, Canada H3A 1B1.
authors.forename: J J
authors.initials: JJ
authors.lastname: Ewbank
authors.forename: T M
authors.initials: TM
authors.lastname: Barnes
authors.forename: B
authors.initials: B
authors.lastname: Lakowski
authors.forename: M
authors.initials: M
authors.lastname: Lussier
authors.forename: H
authors.initials: H
authors.lastname: Bussey
authors.forename: S
authors.initials: S
authors.lastname: Hekimi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins; D016922:Cellular Senescence / Q000235:genetics*; D002874:Chromosome Mapping; D017124:Conserved Sequence; D005091:Exons; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D017238:Genes, Helminth; D005816:Genetic Complementation Test; D005990:Glycerol / Q000378:metabolism; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008136:Longevity / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010641:Phenotype; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012326:RNA Splicing; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C097734:CAT5 protein, S cerevisiae; C104339:CLK-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D005656:Fungal Proteins; D015801:Helminth Proteins; D029701:Saccharomyces cerevisiae Proteins; D005990:Glycerol