title: Development and function of T cells in T cell antigen receptor/CD3 zeta knockout mice reconstituted with Fc epsilon RI gamma.
abstract: Engagement of alpha-beta T cell receptors (TCRs) induces many events in the T cells bearing them. The proteins that transduce these signals to the inside of cells are the TCR-associated CD3 polypeptides and zeta-zeta or zeta-eta dimers. Previous experiments using knockout (KO) mice that lacked zeta (zeta KO) showed that zeta is required for good surface expression of TCRs on almost all T cells and for normal T cell development. Surprisingly, however, in zeta KO mice, a subset of T cells in the gut of both zeta KO and normal mice bore nearly normal levels of TCR on its surface. This was because zeta was replaced by the Fc epsilon RI gamma (FcR gamma). These cells were relatively nonreactive to stimuli via their TCRs. In addition, a previous report showed that zeta replacement by the FcR gamma chain also might occur on T cells in mice bearing tumors long term. Again, these T cells were nonreactive. To understand the consequences of zeta substitution by FcR gamma for T cell development and function in vivo, we produced zeta KO mice expressing FcR gamma in all of their T cells (FcR gamma TG zeta KO mice). In these mice, TCR expression on immature thymocytes was only slightly reduced compared with controls, and thymocyte selection occurred normally and gave rise to functional, mature T cells. Therefore, the nonreactivity of the FcR gamma + lymphocytes in the gut or in tumor-bearing mice must be caused by some other phenomenon. Unexpectedly, the TCR levels of mature T cells in FcR gamma TG zeta KO mice were lower than those of controls. This was particularly true for the CD4+ T cells. We conclude that FcR gamma can replace the functions of zeta in T cell development in vivo but that TCR/CD3 complexes associated with FcR gamma rather than zeta are less well expressed on cells. Also, these results revealed a difference in the regulation of expression of the TCR/CD3 complex on CD4+ and CD8+ T cells.
authors.affiliation: Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Howard Hughes Medical Institute, Denver, CO 80206, USA.
authors.forename: C P
authors.initials: CP
authors.lastname: Liu
authors.forename: W J
authors.initials: WJ
authors.lastname: Lin
authors.forename: M
authors.initials: M
authors.lastname: Huang
authors.forename: J W
authors.initials: JW
authors.lastname: Kappler
authors.forename: P
authors.initials: P
authors.lastname: Marrack
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000276:immunology; D017252:CD3 Complex / Q000737:chemistry* / Q000502:physiology; D002454:Cell Differentiation; D003602:Cytotoxicity, Immunologic; D006410:Hematopoiesis; D007108:Immune Tolerance; D018928:Immunity, Mucosal; D051379:Mice; D018345:Mice, Knockout; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry* / Q000502:physiology; D017455:Receptors, IgE / Q000737:chemistry* / Q000502:physiology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology
chemical_list: D001324:Autoantigens; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D017455:Receptors, IgE