title: [Pneumococcal meningitis resistant to penicillin and nosocomial transmission in pediatric hospitals confirmed by genomic analysis].
abstract: BACKGROUND
  Careful epidemiological studies and sophisticated diagnostic procedures are necessary to prove that bacterial infection is nosocomial in origin. DNA finger printing method can be useful with this aim in view.
  
  
  METHODS
  A 11 month-old girl suffered from a febrile pneumonia. She developed acute meningitis 15 days later; culture of CSF grew Streptococcus pneumoniae, serotype 23 F, resistant to beta-lactamines, erythromycin and cotrimoxazole. She died 24 hours later. Five days after this death, a 5 month-old infant hospitalized in the next bed developed an acute pulmonary infection due to the same strain with the same bacterial characteristics; this patient was cured with cefotaxime plus vancomycin and gentamicin. Randomly amplified polymorphic DNA analysis showed an identical profile of both strains.
  
  
  CONCLUSIONS
  This is the first case of meningitis due to penicillin-resistant Streptococcus pneumoniae (PRSP) associated with nosocomial spread between two children in adjacent beds. This case suggests that it is necessary to isolate patients with PRSP infection during hospitalization.
authors.affiliation: Service de microbiologie, hôpital Saint-Vincent-de-Paul, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Raymond
authors.forename: E
authors.initials: E
authors.lastname: Bingen
authors.forename: N
authors.initials: N
authors.lastname: Brahimi
authors.forename: M
authors.initials: M
authors.lastname: Bergeret
authors.forename: C
authors.initials: C
authors.lastname: Doit
authors.forename: J
authors.initials: J
authors.lastname: Badoual
authors.forename: D
authors.initials: D
authors.lastname: Gendrel
mesh_terms: D003428:Cross Infection; D005260:Female; D006776:Hospitals, Pediatric; D006801:Humans; D007223:Infant; D008586:Meningitis, Pneumococcal / Q000235:genetics / Q000635:transmission*; D008826:Microbial Sensitivity Tests; D010403:Penicillin Resistance; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping