title: The putative LEF-1 proteins from two distinct Choristoneura fumiferana multiple nucleopolyhedroviruses share domain homology to eukaryotic primases.
abstract: We have identified the lef-1 genes from two multiple nucleopolyhedroviruses that infect natural populations of Choristoneura fumiferana. The lef-1 genes in both viruses are directly upstream and in the opposite orientation of their respective ecdysteroid UDP-glucosyltransferase (egt) genes. This gene organization pattern is similar to that found in the genomes of AcMNPV and of OpMNPV. As well, the coding regions and putative protein sequences share a high degree of similarity. Alignment of the predicted amino acid sequences of all known baculovirus lef-1 genes suggests that the LEF-1 proteins have a relatively high degree of conservation, particularly at four identified and distinct domains. Moreover, LEF-1 proteins bear clear similarity to some eukaryotic primases, predominately at three of the four domains where certain amino acids are absolutely conserved.
authors.affiliation: Canadian Forest Service, Sault Ste. Marie, ON, Canada.
authors.forename: J W
authors.initials: JW
authors.lastname: Barrett
authors.forename: H A
authors.initials: HA
authors.lastname: Lauzon
authors.forename: P S
authors.initials: PS
authors.lastname: Mercuri
authors.forename: P J
authors.initials: PJ
authors.lastname: Krell
authors.forename: S S
authors.initials: SS
authors.lastname: Sohi
authors.forename: B M
authors.initials: BM
authors.lastname: Arif
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence; D019915:DNA Primase; D005057:Eukaryotic Cells; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000737:chemistry* / Q000235:genetics; D012316:RNA Nucleotidyltransferases / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D014764:Viral Proteins; C081258:late expression factor 1, baculovirus; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases