title: A study of the aneugenic activity of trichlorfon detected by centromere-specific probes in human lymphoblastoid cell lines.
abstract: The potential of the pesticide trichlorfon to induce mitotic aneuploidy has been investigated in genetically engineered human lymphoblastoid cell lines. Trichlorfon failed to induce micronuclei in the AHH-1 and MCL-5 cell lines when treated in media at normal cell culture pH (pH 7.3). Under a treatment pH of 5.5, trichlorfon exposures resulted in the induction of both chromosome loss and chromosome non-disjunction as measured by fluorescence in situ hybridisation (FISH) using a pan-centromeric probe for all human centromeres and centromere probes specific for chromosomes 2, 7 and 18. At treatment concentrations greater than 20 micrograms/ml trichlorfon also induced structural chromosome damage resulting in the production of centromere negative micronuclei.
authors.affiliation: School of Biological Sciences, University of Wales, Swansea, UK.
authors.forename: A T
authors.initials: AT
authors.lastname: Doherty
authors.forename: S
authors.initials: S
authors.lastname: Ellard
authors.forename: E M
authors.initials: EM
authors.lastname: Parry
authors.forename: J M
authors.initials: JM
authors.lastname: Parry
mesh_terms: D000782:Aneuploidy; D001402:B-Lymphocytes; D002460:Cell Line; D002503:Centromere; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007306:Insecticides / Q000633:toxicity*; D015162:Micronucleus Tests; D009630:Nondisjunction, Genetic; D014236:Trichlorfon / Q000633:toxicity*
chemical_list: D015342:DNA Probes; D007306:Insecticides; D014236:Trichlorfon