Transfer of attaching and effacing from a strain of enteropathogenic Escherichia coli to E. coli K-12.
The ability to cause attaching and effacing (AE) lesions in intestinal epithelial cells is an essential virulence trait of enteropathogenic E. coli (EPEC) that requires several chromosomal genes acting in concert with one another. In this study, we show that the ability to cause AE lesions can be transferred by conjugal mating from a high frequency recombinant (Hfr) derivative of a rabbit EPEC strain, E. coli RDEC-1, to a strain of E. coli K-12. Although the recipient acquired a considerable amount of donor DNA during the transfer process, it expressed the AE phenotype phenotype only weakly. The findings suggest the AE is a multigene phenomenon, the genes for which may not reside on a single region of the bacterial chromosome.
O'Gorman
L E
LE
Department of Microbiology and Infectious Diseases, Royal Children's Hospital, Australia.
Krejany
E O
EO
Bennett-Wood
V R
VR
Robins-Browne
R M
RM
D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism; D015139:Blotting, Southern; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000382:microbiology / Q000648:ultrastructure; D004926:Escherichia coli / Q000472:pathogenicity*; D015964:Gene Expression Regulation, Bacterial; D018014:Gene Transfer Techniques; D007082:Ileum / Q000382:microbiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D010957:Plasmids; D011817:Rabbits; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial