In vivo effects of fluconazole on lymphocyte subpopulations of the thymus and spleen in mice: flow cytometry analysis.
Fluconazole is a triazole compound developed for the therapy of fungal infections, especially for systemic fungal infections. In this study 2.5 and 5.0 mg oral doses of fluconazole per kg of body weight were administered to mice for 3-28 days, followed by lymphocyte phenotyping by flow cytometry analysis on a Becton-Dickinson FACScan. The results show that administration of fluconazole neither reduced body and organ weights nor thymocyte and spleen cell count. In the thymus and spleen the distribution of all the lymphocyte subpopulations studied was unchanged. These results suggest that fluconazole has no in vivo effects on the quantity of lymphocyte subpopulations in mice. The results tend to support the use of fluconazole in immunosuppressed patients.
Liu
J
J
Institute of Pharmacology and Toxicology, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Mannheim, Germany.
Mengel
K
K
Friedberg
K D
KD
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D017252:CD3 Complex / Q000096:biosynthesis; D015704:CD4 Antigens / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D015725:Fluconazole / Q000008:administration & dosage / Q000494:pharmacology*; D007441:Intubation, Gastrointestinal; D018655:Lymphocyte Count / Q000187:drug effects; D016131:Lymphocyte Subsets / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D011947:Receptors, Antigen, B-Cell / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013950:Thymus Gland / Q000166:cytology* / Q000187:drug effects
D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D011947:Receptors, Antigen, B-Cell; D015725:Fluconazole