title: Isolation and characterization of chitinase from a flake-chitin degrading marine bacterium, Aeromonas hydrophila H-2330.
abstract: A flake-chitin degrading marine bacterium was isolated and identified as Aeromonas hydrophila. This strain secreted five chitinases and an beta-N-acetylglucosaminidase. The main chitinase (Chi-A) was purified and characterized. The optimum pH of Chi-A was 5-8, and the activity was inhibited by Hg2+ and Fe3+. Chi-A was different from chitinases of other Aeromonas species with respect to molecular weight (62,000) and insensitivity to monoiodoacetate. The amino-terminal amino acid sequence showed extensive similarity with chitinases from Gram-negative bacteria.
authors.affiliation: Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hiraga
authors.forename: L
authors.initials: L
authors.lastname: Shou
authors.forename: M
authors.initials: M
authors.lastname: Kitazawa
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Shimada
authors.forename: R
authors.initials: R
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Oda
mesh_terms: D016980:Aeromonas hydrophila / Q000737:chemistry / Q000145:classification / Q000201:enzymology*; D000595:Amino Acid Sequence; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D002686:Chitin; D002688:Chitinases