Electrically induced NGF production by astroglial cells.
A controlled culture system has been developed to induce nerve growth factor (NGF) production in astroglial cells that are cultured on an electrode surface. The electrode potential is alternatively modulated at an amplitude of 300 mV and a frequency of 10 Hz. The electric stimulation triggers NGF production and secretion. The mechanism of the electrically induced NGF production is discussed in association with protein kinase C (PKC) activation.
Koyama
S
S
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Haruyama
T
T
Kobatake
E
E
Aizawa
M
M
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009414:Nerve Growth Factors / Q000096:biosynthesis*
D009414:Nerve Growth Factors; D002118:Calcium