Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome.
The tylLM region of the tylosin biosynthetic gene cluster of Streptomyces fradiae contains four open reading frames (orfs1*-4*). The function of the orf1* product is not known. The product of orf2* (tylM2) is the glycosyltransferase that adds mycaminose to the 5-hydroxyl group of tylactone, the polyketide aglycone of tylosin (Ty). A methyltransferase, responsible for 3-N-methylation during mycaminose production, is encoded by orf3* (tylM1). The product of orf4* (cer) is crotonyl-CoA reductase, which converts acetoacetyl-CoA to butyryl-CoA for use as a 4C extender unit during tylactone production.
Gandecha
A R
AR
Department of Biochemistry, University of Leicester, UK.
Large
S L
SL
Cundliffe
E
E
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005944:Glucosamine / Q000031:analogs & derivatives / Q000096:biosynthesis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D005810:Multigene Family; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D015645:Tylosin / Q000096:biosynthesis* / Q000737:chemistry
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C013610:mycaminose; D005944:Glucosamine; D015645:Tylosin