Structural polypeptides of different clinical strains of avian adenovirus type-1.
The polypeptides of two liver strains, two ovarian strains and one standard strain of avian adenovirus-1 (AAV-1) were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot using polyclonal antibodies to all the strains and isoelectric focusing (IEF). All the strains had 11 polypeptides and molecular weight varied between 9 and 100 kDa. The polypeptide pattern was similar in all the strains as revealed by SDS-PAGE and Western blot. However, some differences among the strains on the basis of isoelectric point of polypeptide were observed by IEF.
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Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, Bidhan Chandra Krishi Viswavidylaya, Mohanpur, Nadia, India.
Sarkar
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D000914:Antibodies, Viral / Q000276:immunology; D000259:Aviadenovirus / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008854:Microscopy, Electron; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification*
D000914:Antibodies, Viral; D014764:Viral Proteins