title: A contribution to the systematization of bovine herpesvirus 1 based on genomic mapping by restriction fragment pattern analysis.
abstract: Fourteen isolates of bovine herpesvirus 1 (BHV-1) found representative of more than 100 isolates studied, were compared by restriction fragment pattern analyses and molecularly characterized. A number of evolutionary links between the variants originally associated with infectious bovine rhinotracheitis and the variants originally associated with infectious pustular vulvovaginitis were identified. These findings, as well as the lack of any correlation between genome type and clinical manifestation, confirm that there is no phylogenetic basis for a distinction between groups of strains associated with genital and respiratory disease. Two attenuated vaccine strains can be identified as deviating from field isolates.
authors.affiliation: Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark.
authors.forename: L S
authors.initials: LS
authors.lastname: Christensen
authors.forename: K G
authors.initials: KG
authors.lastname: Madsen
authors.forename: B
authors.initials: B
authors.lastname: Nylin
authors.forename: L
authors.initials: L
authors.lastname: Rønsholt
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D002417:Cattle; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D016679:Genome, Viral; D007242:Herpesvirus 1, Bovine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015183:Restriction Mapping / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D015247:Deoxyribonuclease HindIII; C024406:endodeoxyribonuclease HpaI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific