title: Adenovirus E1a interferes with expression of vaccinia viral genes.
abstract: The 12S and 13S cDNAs of the oncogene E1a encoded by the early region of adenovirus 12 (Ad12) were overexpressed using the T7/encephalomyocarditis (EMC)/vaccinia hybrid expression system. The E1a proteins were stable for at least 12 h in monkey epithelial BSC1 cells. The E1a proteins were recognized by a rabbit polyclonal antibody and displayed phosphorylation patterns similar to those displayed by the E1a proteins expressed in Ad12-transformed cells. Expression of E1a proteins by recombinant vaccinia virus led to inhibition of vaccinia viral protein synthesis which was observed as soon as 6 h after infection. This suppression was mediated by both the 12S and the 13S products of Ad12E1a and to a somewhat lesser extent by the 13S product of Ad2E1a. The inhibition of vaccinia virus gene expression resulted in enhanced survival of vaccinia virus-infected cells. These results suggest that the proteins encoded by the E1a sequester a viral or a cellular product(s) that is essential for the expression of vaccinia virus-encoded genes.
authors.affiliation: Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Strauss
authors.forename: O
authors.initials: O
authors.lastname: Elroy-Stein
authors.forename: R
authors.initials: R
authors.lastname: Ehrlich
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D017500:Adenovirus E1A Proteins / Q000235:genetics*; D017398:Alternative Splicing; D002461:Cell Line, Transformed; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D011499:Protein Processing, Post-Translational; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics*; D014762:Viral Interference; D014764:Viral Proteins
chemical_list: D017500:Adenovirus E1A Proteins; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases