title: Distribution of Glut1 glucose transporters in different brain structures compared to glucose utilization and capillary density of adult rat brains.
abstract: Glut1 is a specific transporter system that mediates glucose transfer across the blood-brain barrier (BBB). Although the main location of Glut1 is in the capillary endothelium of the brain, its local distribution in different brain regions is not as well defined. In the present investigation, the local pattern of Glut1 distribution was determined in 13 brain structures using an immunoautoradiographic method developed for this purpose. A polyclonal antibody directed against the C-terminal amino acid sequence of Glut1 was applied to cryosections of rat brains. A secondary antibody was added that had been coupled to [35S]. Results show a heterogeneous distribution of Glut1 in the brain with activities of [35S] ranging from 65% below to 15% above the mean. White matter activity was lower than gray matter activity. For comparison, capillary sections were counted in corresponding cryosections by indirect immunofluorescence using fibronectin antibodies. In addition, local cerebral glucose utilization (LCGU) was analyzed in identical brain structures of conscious rats by the quantitative autoradiographic 2-deoxyglucose method. Significant correlations were found between Glut1 density and either LCGU or capillary density. Results indicate a tight coupling of Glut1 transporter density and capillary density to the LCGU of different BBB structures in adult rats.
authors.affiliation: Department of Physiology, University of Heidelberg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Zeller
authors.forename: S
authors.initials: S
authors.lastname: Rahner-Welsch
authors.forename: W
authors.initials: W
authors.lastname: Kuschinsky
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply* / Q000378:metabolism; D001923:Brain Chemistry; D002196:Capillaries; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; C495679:Slc2a1 protein, rat; D005947:Glucose