title: Identification of a human endogenous LTR-like sequence using HIV-1 LTR specific primers.
abstract: Using 'consensus' primers derived from the LTR region of 15 HIV-1 isolates, a fragment of 583 bp was amplified from human DNA. Even though specificity was confirmed by Southern blot analysis with a conserved LTR oligonucleotide probe, no significant homologies were detected to either retroviral regions or human or non-human published sequences. Nevertheless, when used as a probe, the 583-bp fragment identified a unique DNA sequence in the human genome on chromosome 1, and cross-reactive sequences in monkey, but not mouse, DNA. This novel, unique and conserved sequence of 583 bp was used to isolate a human HS-1 clone in which the structural property of a viral LTR could be identified.
authors.affiliation: Istituto di Oncologia, University of Padova, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Menin
authors.forename: S
authors.initials: S
authors.lastname: Indraccolo
authors.forename: M
authors.initials: M
authors.lastname: Montagna
authors.forename: B
authors.initials: B
authors.lastname: Corneo
authors.forename: L
authors.initials: L
authors.lastname: Bonaldi
authors.forename: C
authors.initials: C
authors.lastname: Leib-Mösch
authors.forename: L
authors.initials: L
authors.lastname: Chieco-Bianchi
authors.forename: E
authors.initials: E
authors.lastname: D'Andrea
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004247:DNA