[Complex-formation of phospholipase A2 with fragments of nucleic acids and approaches to its elimination].
Formation of phospholipase A2 stable complexes with nucleic acid fragments was observed upon the isolation of the enzyme from pig pancreas. This complex could be disrupted by neither DNA precipitation with protamine chloride and cetyltrimethylammonium bromide nor chromatography on DEAE-cellulose and CM-cellulose or hydroxyapatite. The treatment of the complexes with 1 M acetic acid followed by gel-chromatography on Sephadex G-75 in 1 M acetic acid resulted in complete separation of active phospholipase A2 from DNA fragments.
Litvinko
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Drozhdeniuk
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AP
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D053938:DNA Fragmentation; D010179:Pancreas / Q000201:enzymology; D010741:Phospholipases A / Q000737:chemistry / Q000302:isolation & purification*; D054467:Phospholipases A2; D013552:Swine
D004247:DNA; D010741:Phospholipases A; D054467:Phospholipases A2