Inhibition of self-splicing group I intron RNA: high-throughput screening assays.
High-throughput screening assays have been developed to rapidly identify small molecule inhibitors targeting catalytic group I introns. Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis carinii or from bacteriophage T4 have been investigated. In vitro biochemical assays amenable to high-throughput screening have been established. Small molecules that inhibit the functions of group I introns have been identified. These inhibitors should be useful in better understanding ribozyme catalysis or in therapeutic intervention of group I intron-containing microorganisms.
Mei
H Y
HY
Department of Chemistry, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, MI 48106, USA. meih@aa.wl.com
Cui
M
M
Sutton
S T
ST
Truong
H N
HN
Chung
F Z
FZ
Czarnik
A W
AW
D017122:Bacteriophage T4 / Q000235:genetics; D002384:Catalysis; D007438:Introns; D011010:Pneumocystis / Q000235:genetics; D012313:RNA / Q000235:genetics*; D012326:RNA Splicing
D012313:RNA