title: Neutrophil margination, sequestration, and emigration in the lungs of L-selectin-deficient mice.
abstract: These studies tested the hypothesis that L-selectin plays a role in neutrophil traffic in the lungs, particularly in neutrophil margination, sequestration, and emigration, using L-selectin-deficient mice. No defect in neutrophil margination within either capillaries or arterioles and venules was observed in uninflamed lungs of L-selectin-deficient mice. The initial rapid sequestration of neutrophils within the pulmonary capillaries 1 min after intravascular injection of complement fragments was not prevented. In contrast, L-selectin did contribute to the prolonged neutrophil sequestration (> or = 5 min). Interestingly, neutrophil accumulation within noncapillary microvessels required L-selectin at both 1 and 5 min after complement injection. During bacterial pneumonias, L-selectin played a role in neutrophil accumulation within noncapillary microvessels in response to either Escherichia coli or Streptococcus pneumoniae and within capillaries in response to E. coli but not S. pneumoniae. However, L-selectin was not required for emigration of neutrophils or edema in response to either organism. These studies demonstrate a role for L-selectin in the prolonged sequestration of neutrophils in response to intravascular complement fragments, in the intracapillary accumulation of neutrophils during E. coli-induced pneumonia, and in the accumulation of neutrophils within noncapillary microvessels when induced by either intravascular complement fragments or
authors.affiliation: Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
authors.forename: N A
authors.initials: NA
authors.lastname: Doyle
authors.forename: S D
authors.initials: SD
authors.lastname: Bhagwan
authors.forename: B B
authors.initials: BB
authors.lastname: Meek
authors.forename: G J
authors.initials: GJ
authors.lastname: Kutkoski
authors.forename: D A
authors.initials: DA
authors.lastname: Steeber
authors.forename: T F
authors.initials: TF
authors.lastname: Tedder
authors.forename: C M
authors.initials: CM
authors.lastname: Doerschuk
mesh_terms: D000818:Animals; D001160:Arterioles / Q000276:immunology; D002196:Capillaries / Q000276:immunology; D002448:Cell Adhesion / Q000235:genetics* / Q000276:immunology*; D002465:Cell Movement / Q000235:genetics* / Q000276:immunology*; D003165:Complement System Proteins / Q000494:pharmacology; D004487:Edema / Q000276:immunology; D004926:Escherichia coli; D007249:Inflammation / Q000235:genetics*; D019041:L-Selectin / Q000235:genetics*; D007958:Leukocyte Count; D008168:Lung / Q000276:immunology*; D051379:Mice; D008817:Mice, Mutant Strains; D016296:Mutagenesis; D009504:Neutrophils / Q000276:immunology* / Q000502:physiology*; D018410:Pneumonia, Bacterial / Q000276:immunology; D013296:Streptococcus pneumoniae; D014699:Venules / Q000276:immunology
chemical_list: D019041:L-Selectin; D003165:Complement System Proteins