Mapping and characterization of the origin of DNA replication of porcine circovirus.
The origin of DNA replication of porcine circovirus (PCV) was mapped to a 111-bp fragment. On top of a hairpin, a nonanucleotide (TAGTATTAC) homologous to nonanucleotides of other viruses was identified. Mutation of this element abolishes replication. PCV may be related to a virus family characterized by single-stranded circular DNA genomes, rolling-circle replication, and homology of their rep proteins.
Mankertz
A
A
Fachbereich Gentechnik und Genetik, Robert Koch-Institut, Berlin, Germany.
Persson
F
F
Mankertz
J
J
Blaess
G
G
Buhk
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D017925:Circovirus / Q000235:genetics*; D004279:DNA, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D018741:Replication Origin; D017386:Sequence Homology, Amino Acid; D013552:Swine
D004279:DNA, Viral