title: Cytochalasin J treatment significantly alters mitotic spindle microtubule organization and kinetochore structure in PtK1 cells.
abstract: It has previously been demonstrated that treatment of mitotic PtK1 cells with 10-20 microg/ml cytochalasin J (CJ) blocks or slows chromosome motion and has a significant effect on spindle architecture [Snyder and Cohen, 1995: Cell Motil. Cytoskeleton 32:245-257]. Spindle microtubules (MTs) were shown to reorganize within the spindle domain, with kinetochore MTs (kMTs) reduced in number and non-kinetochore MTs (nkMTs) shown to splay outside the original spindle domain. In some cases, bundles of MTs were shown to be refocused away from the original spindle poles, creating the appearance of a multi-polar spindle. In this paper we use serial section electron microscopy, coupled with computer-assisted reconstruction techniques, to determine the rearrangement of spindle MTs and chromosome position following brief treatments of mitotic cells with 10-20 microg/ml CJ at various stages of mitosis. CJ treatment of prometaphase cells reduces the number of kMTs and the size and organization of the kinetochore lamina. Instead of kinetochore bundles of MTs aligned parallel to one another and running from kinetochore to pole, this class of MTs is highly fragmented. Non-kinetochore MTs are also highly fragmented, usually less than 2 microm long, and remain relatively straight over short distances, with some MTs arranged at an oblique angle to the longitudinal spindle axis. In approximately 30% of cells treated with CJ, the failure of a small number of chromosomes to attach to spindle fibers can be documented. These chromosomes show a significant change in the organization of the kinetochore laminae. Light microscopic analysis of cells treated with CJ reveals loss of chromosome congression, with chromsomes usually located at the periphery of the spindle and some completely detached from the spindle. Cells treated with 10 microg/ml CJ for 10 min and released into tissue culture medium show a resumption of chromosome motion within a few minutes, both during congression and anaphase. Where kMTs are inserted into kinetochores, chromosome motion is seen; where chromosomes fail to attach to the spindle, no chromosome motion is observed. Cells treated in metaphase show a delayed entry into anaphase and a reduced rate of anaphase A, with the arms of some chromosomes remaining in the interzone region. Our results suggest that CJ-sensitive molecules play a role in the organization of spindle MTs, as well as their functional association to kinetochores.
authors.affiliation: Department of Biological Sciences, University of Denver, CO 80208, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Wrench
authors.forename: J A
authors.initials: JA
authors.lastname: Snyder
mesh_terms: D000818:Animals; D002460:Cell Line; D003572:Cytochalasins / Q000494:pharmacology*; D007091:Image Processing, Computer-Assisted; D018386:Kinetochores / Q000187:drug effects* / Q000648:ultrastructure; D008394:Marsupialia; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008941:Spindle Apparatus / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D003572:Cytochalasins; C106137:cytochalasin J