In vitro effects of hyperthermia combined with cisplatin or peplomycin on the human maxillary carcinoma cell line IMC-2.
We examined the interactive effects of hyperthermia combined with cisplatin (CDDP) (0.5 micrograms/ml) or peplomycin (PEP) (1.0 microgram/ml) on surviving fractions of human maxillary carcinoma IMC-2 cells. Either CDDP or PEP enhanced the 44 degree C thermosensitivity of thermotolerant cells after heating at 42 degrees C for 2 hours. The development of thermotolerance at 42 degrees C with either of the two drugs for 2 hours was not inhibited by CDDP, but it was partially inhibited by PEP. Moreover, for PEP throughout the entire period of 42-44 degrees C step-up heating, the 44 degree C thermosensitivity of thermotolerant cells after heating at 42 degrees C with PEP for 2 hours was enhanced similarly to that at 44 degrees C with PEP. Heating at 42 degrees C combined with either of the two drugs showed a marked interactive effect.
Ohtsubo
T
T
Department of Otorhinolaryngology, Fukui Medical University, Japan.
Saito
H
H
Matsumoto
H
H
Hayashi
S
S
Shioura
H
H
Kitai
R
R
Saito
T
T
Kano
E
E
D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology* / Q000627:therapeutic use; D003131:Combined Modality Therapy; D006801:Humans; D006979:Hyperthermia, Induced; D008441:Maxillary Neoplasms / Q000473:pathology* / Q000628:therapy; D017663:Peplomycin / Q000494:pharmacology* / Q000627:therapeutic use; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D017663:Peplomycin; D002945:Cisplatin