Degeneration of Cajal-Retzius cells in the developing cerebral cortex of the mouse after ablation of meningeal cells by 6-hydroxydopamine.
In the central nervous system, the neurotoxic drug 6-hydroxydopamine (6-OHDA) selectively deletes central catecholaminergic neurons and meningeal cells. Meningeal cells are known to contribute to brain development and their specific degeneration leads to disorganized neuronal positioning. We have analyzed whether a particular population of cortical pioneer neurons, the Cajal-Retzius (CR) cells, which lie just below meningeal cells, is also affected by 6-OHDA treatment. We show that application of 6-OHDA to the cortical surface leads to a rapid degeneration of CR cells, without affecting other cortical neurons. The ablation of CR cells was prevented by normetanephrine, which blocks the 6-OHDA uptake into meningeal cells. These results indicate that the disappearance of CR cells after 6-OHDA treatment may be a result of the ablation of the meningeal cells and suggest a trophic dependence of CR cells upon meningeal cells.
Supèr
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Department of Animal and Plant Cell Biology, Faculty of Biology, University of Barcelona, Spain.
Martínez
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Soriano
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D000818:Animals; D064032:Calbindin 2; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology* / Q000254:growth & development*; D007150:Immunohistochemistry; D008578:Meninges / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D016627:Oxidopamine; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D013565:Sympatholytics
D064032:Calbindin 2; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G; D013565:Sympatholytics; D016627:Oxidopamine