Tolerogenic capacity of poly(ethylene glycol) (PEG)--modified ovalbumins in relation to their immunoreactivity towards anti-ovalbumin antibody.
Ovalbumin (OVA) was chemically modified with poly(ethylene glycol) (PEG) derivatives: activated PEG2, 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine and activated PM13, copolymer of poly(oxyethylene) allyl methyl diether and maleic anhydride. Pretreatment of BALB/c mice with PEG2- or PM13-OVA suppressed the production of both IgG- and IgE-class anti-OVA antibodies induced by subsequent immunizations with unmodified OVA. PEG2-OVA had higher potency to suppress OVA-specific immune response than PM13-OVA. Although extensive modification (62%) of amino groups in the OVA molecule with activated PEG2 was required to diminish most of its immunoreactivity towards anti-OVA antibodies, only a low degree of modification (27%) was sufficient to induce tolerogenicity to OVA. Thus, the loss of immunoreactivity of PEG2-OVA was not prerequisite for its tolerogenic capacity. Moreover, the use of completely denatured PEG2-OVA immunogen lead to the loss of its ability to induce immune tolerance to native OVA. These observations are discussed in relation to the regulatory mechanism of immune response.
Saito
T
T
Toin Human Science and Technology Center, Department of Materials Science and Technology, Toin University of Yokohama, Japan.
Nishimura
H
H
Sekine
T
T
Urushibara
T
T
Kodera
Y
Y
Hiroto
M
M
Matsushima
A
A
Inada
Y
Y
D000818:Animals; D000906:Antibodies; D001672:Biocompatible Materials / Q000737:chemistry; D002645:Chickens; D005260:Female; D007108:Immune Tolerance; D007114:Immunization; D008297:Male; D008299:Maleic Anhydrides / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology*; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; D001672:Biocompatible Materials; D008299:Maleic Anhydrides; C054144:polyoxyethylene allylmethyl diether-maleic anhydride copolymer; D011092:Polyethylene Glycols; D010047:Ovalbumin