Variability of mitochondrial subgenomic molecules in the meristematic cells of higher plants.
MtDNAs from BY-2 cells and rice root were analyzed by random amplified polymorphic DNA (RAPD) assay and Southern hybridization analysis. A number of differences were observed in the RAPD patterns amplified from mtDNAs sampled at different phases of the BY-2 cell culture. RAPD fragments also varied with the template DNAs derived from various areas of rice root tip. When a RAPD fragment was hybridized to restriction fragments of whole DNAs, isolated from the distal area of the apical meristem and differentiated elongation zone of a root, two distinct stoichiometric differences were observed in the hybridization signals. This suggests that the organization of mt-genome in prototypic cells in the root apical meristem differs from that found in the differentiated cells.
Suzuki
T
T
Department of Plant Sciences, Graduate School of Science, University of Tokyo, Japan.
Kawano
S
S
Sakai
A
A
Hirai
A
A
Kuroiwa
T
T
D015139:Blotting, Southern; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D063245:Plant Development; D018517:Plant Roots / Q000235:genetics; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction / Q000379:methods*; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000254:growth & development
D004272:DNA, Mitochondrial; D018744:DNA, Plant