title: Ozone depletes tocopherols and tocotrienols topically applied to murine skin.
abstract: To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG-treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRF-treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Thiele
authors.forename: M G
authors.initials: MG
authors.lastname: Traber
authors.forename: M
authors.initials: M
authors.lastname: Podda
authors.forename: K
authors.initials: K
authors.lastname: Tsang
authors.forename: C E
authors.initials: CE
authors.lastname: Cross
authors.forename: L
authors.initials: L
authors.lastname: Packer
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D005260:Female; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D051379:Mice; D008812:Mice, Hairless; D010083:Oxidants, Photochemical / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity*; D000073878:Palm Oil; D010938:Plant Oils; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010083:Oxidants, Photochemical; D010938:Plant Oils; D014810:Vitamin E; D000073878:Palm Oil; D010126:Ozone