Detection of IgG antibody to Epstein-Barr virus viral capsid antigen in saliva by antibody capture radioimmunoassay.
A 'G' antibody capture radioimmunoassay (GACRIA) to detect IgG to Epstein-Barr virus (EBV) viral capsid antigen (VCA) in saliva is described. The monoclonal antibody to EBV VCA used in the GACRIA bound non-specifically when testing saliva samples having a total IgG content of less than 2.0 mg/l, so giving false positive results. This problem was overcome by including 0.5% EBV-negative human serum in the monoclonal antibody diluent. The performance of the assay was then evaluated by comparing the GACRIA test on serum and saliva samples to indirect immunofluorescence assay (IFA) results on sera using a panel of paired serum/saliva samples. Compared to the corresponding serum IFA the saliva GACRIA had a sensitivity and specificity of 93.5 and 100%, respectively. Although less sensitive than IFA on serum samples, the saliva GACRIA is sufficiently sensitive to be used for epidemiological screening and will enable testing for anti-EBV VCA to be carried out easily and on a wide scale.
Vyse
A J
AJ
Enteric and Respiratory Virus Laboratory, London, UK.
Knowles
W A
WA
Cohen
B J
BJ
Brown
D W
DW
D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006566:Herpesviridae Infections / Q000276:immunology / Q000821:virology; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D012463:Saliva / Q000276:immunology / Q000821:virology*; D014412:Tumor Virus Infections / Q000276:immunology / Q000821:virology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G