Acute exposure to ozone does not influence neuroreceptor density and sensitivity in guinea pig lung.
The effects of acute exposure of guinea pigs to 3 ppm of ozone for 2 h on the receptor density and sensitivity of the muscarinergic-, the histaminergic- and the beta-adrenergic receptor systems were studied, in order to provide more insight in the complex mechanisms underlying the well known ozone-induced changes in receptor functionality. The exposure to ozone did not change either the total amount of receptors present in lung tissue, nor the receptor sensitivity of the systems studied. Although no effects were observed, this does not yet fully exclude the receptor system for being a target of ozone exposure. The receptor function can be changed after exposure to ozone, e.g., the coupling with the G-protein can be influenced. Furthermore, the G-protein itself may have been altered or changes can occur at lower levels in the receptor signal transmission route leading to functional changes after stimulation of the receptor with an agonist.
van Hoof
H J
HJ
Leiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, The Netherlands. vanhoof@chem.vu.nl
van Acker
F A
FA
Voss
H P
HP
van Bree
L
L
Bast
A
A
D000818:Animals; D006168:Guinea Pigs; D008168:Lung / Q000187:drug effects*; D008297:Male; D010126:Ozone / Q000633:toxicity*; D011485:Protein Binding / Q000187:drug effects; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism; D011969:Receptors, Histamine H1 / Q000187:drug effects / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000187:drug effects* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
D011943:Receptors, Adrenergic, beta; D011969:Receptors, Histamine H1; D011976:Receptors, Muscarinic; D010126:Ozone