Use of siderophores to type pseudomonads: the three Pseudomonas aeruginosa pyoverdine systems.
Eighty-eight Pseudomonas aeruginosa isolates, most of them from the Collection of Bacterial Strains of the Institut Pasteur, Paris, were analysed for their pyoverdine-mediated iron incorporation system by different methods, including pyoverdine isoelectrofocusing analysis, pyoverdine-mediated growth stimulation, immunoblot detection of (ferri)pyoverdine outer-membrane receptor and pyoverdine-facilitated iron uptake. The same grouping of the strains was reached by each of these methods, resulting in the classification of the P. aeruginosa isolates, even those which were devoid of pyoverdine production, into three different siderophore types. Forty-two percent of the strains were identified with the type-strain P. aeruginosa ATCC 15,692 (group I), 42% were identical with the second type-strain P. aeruginosa ATCC 27,853 (group II) and 16% reacted identically with the clinical isolate P. aeruginosa Pa6, whose pyoverdine was recognized in this study to be identical in structure to the pyoverdine produced by a natural isolate, P. aeruginosa strain R. No new pyoverdine species was detected among these strains.
Meyer
Jean-Marie
JM
Laboratoire de Microbiologie et de Génétique, Université Louis-Pasteur, Unité de Recherche Associée au Centre National de la Recherche Scientifique No. 1481, 28 rue Goethe, F-67000 Strasbourg, France.
Stintzi
Alain
A
Laboratoire de Microbiologie et de Génétique, Université Louis-Pasteur, Unité de Recherche Associée au Centre National de la Recherche Scientifique No. 1481, 28 rue Goethe, F-67000 Strasbourg, France.
De Vos
Daniel
D
Vlaams Interuniversitair Instituut Biotechnologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 St-Genesius-Rode, Belgium.
Cornelis
Pierre
P
Vlaams Interuniversitair Instituut Biotechnologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 St-Genesius-Rode, Belgium.
Tappe
Robert
R
Institut für Organische Chemie, Universität zu Köln, Greinstrasse 4, D-50939 Köln, Germany.
Taraz
Kambiz
K
Institut für Organische Chemie, Universität zu Köln, Greinstrasse 4, D-50939 Köln, Germany.
Budzikiewicz
Herbert
H
Institut für Organische Chemie, Universität zu Köln, Greinstrasse 4, D-50939 Köln, Germany.
D001425:Bacterial Outer Membrane Proteins / Q000145:classification / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001692:Biological Transport; D007501:Iron / Q000378:metabolism*; D009842:Oligopeptides; D010860:Pigments, Biological / Q000737:chemistry / Q000145:classification* / Q000378:metabolism / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000187:drug effects / Q000254:growth & development; D017262:Siderophores / Q000737:chemistry / Q000145:classification* / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; C082326:FpvA protein, Pseudomonas aeruginosa; D009842:Oligopeptides; D010860:Pigments, Biological; D017262:Siderophores; C042453:pyoverdin; D007501:Iron