title: [Use of aerosol A-300 amd GF/F (GF/C) filters for purifying fragments of DNA, plasmid DNA, and RNA].
abstract: Aerosil A-300 and GF/F (GF/C) filters were used for purifying plasmid DNA and intact RNA as well as for the recovery of DNA fractionated on agarose gels. In the presence of guanidinium thiocyanate Aerosil A-300 and GF/F (GF/C) filters selectively bind nucleic acids, while proteins, agarose polysaccharides and salts are washed off. Nucleic acids desorbed from the Aerosil and filters are available immediately as substrates: DNA for restriction analysis, ligation and sequencing; RNA--for RT-PCR.
authors.forename: P G
authors.initials: PG
authors.lastname: Gribanov
authors.forename: A V
authors.initials: AV
authors.lastname: Shcherbakov
authors.forename: N A
authors.initials: NA
authors.lastname: Perevozchikova
authors.forename: A A
authors.initials: AA
authors.lastname: Gusev
mesh_terms: D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004274:DNA, Recombinant / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D008970:Molecular Weight; D010957:Plasmids; D012313:RNA / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004274:DNA, Recombinant; D012313:RNA; D012822:Silicon Dioxide; D004247:DNA