Cloning and characterization of a Neurospora crassa ribosomal protein gene, crps-7.
A Neurospora crassa ribosomal protein gene, crps-7, was isolated from a genomic DNA library closely linked to the morphological gene ro-2. Sequence analysis and a computerized database search revealed a high degree of homology to the Xenopus laevis rps8 and rat rps7 gene, as well as to uncharacterized ORFs from two yeast species. Comparison with a nearly full-length cDNA clone revealed two introns, one of which is in a conserved position shared with the Xenopus gene. Although a number of sequence motifs common to other N. crassa ribosomal protein genes are present upstream of the crps-7 gene, mRNA abundance is not tightly regulated by carbon availability. Relative transcript levels during nitrogen limitation and thermal stress were also examined.
Vierula
P J
PJ
Department of Biology, Carleton University, Ottawa, Ontario K1S 5B6, Canada.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002244:Carbon / Q000494:pharmacology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D018076:DNA, Complementary / Q000235:genetics; D005656:Fungal Proteins; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D016681:Genome, Fungal; D018869:Heat-Shock Response; D007438:Introns; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D016366:Open Reading Frames; D012331:RNA, Fungal / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012269:Ribosomal Proteins / Q000235:genetics*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013395:Sucrose / Q000378:metabolism; D014158:Transcription, Genetic; D014982:Xenopus laevis
D018076:DNA, Complementary; D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C105221:crps-7 protein, Neurospora crassa; D013395:Sucrose; D002244:Carbon; D009584:Nitrogen