Two-dimensional gel analysis of [35S]methionine labelled and phosphorylated proteins present in virions and light particles of herpes simplex virus type 1, and detection of potentially new structural proteins.
Cells infected with herpes simplex virus (HSV) synthesize both infectious viruses and non-infectious light particles (L-particles). The latter contain the envelope and tegument components of the virions, but lack virus capsid and DNA. Electrophoresis in SDS-polyacrylamide gels (SDS-PAGE) has been used extensively for analysis of structural proteins in virions and L-particles. Two-dimensional (2-D) gel electrophoresis, however has a markedly higher resolution, and in the present work we have used this technique to study both [35S]methionine labelled and phosphorylated structural proteins in virions and L-particles. Proteins were assigned to the tegument or the envelope by the analysis of L-particles. Localization of structural proteins was also determined by stepwise solubilization in the presence of the neutral detergent NP-40 and NaCl, and by isolation of capsids from nuclei of infected cells. Different steps in posttranslational modification can be detected by 2-D gel electrophoresis such that a single polypeptide may appear as several spots. This was most clearly observed for some of the HSV-encoded glycoproteins which were shown to exist in multiple forms in the virion. Some polypeptides apparently not identified previously were either capsid associated, or localized in the tegument or envelope. The degrees of phosphorylation in L-particles and virions are almost identical for some proteins, but markedly different for others. Thus, glycoprotein E of HSV-1 is for the first time shown to be phosphorylated, and most heavily so in virions. The IE VMW)110 protein represents a group of proteins which are more phosphorylated in L-particles than in virions. Attempts are made to correlate the proteins detected by 2-D analysis with those previously separated by SDS-PAGE.
Sathananthan
B
B
Bergen High Technology Centre, University of Bergen, Norway.
Rødahl
E
E
Ekberg
T
T
Langeland
N
N
Haarr
L
L
D000818:Animals; D002213:Capsid / Q000302:isolation & purification; D006224:Cricetinae; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D018259:Herpesvirus 1, Human / Q000737:chemistry* / Q000378:metabolism*; D007668:Kidney; D008715:Methionine / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate; D012995:Solubility; D013462:Sulfur Radioisotopes; D015678:Viral Structural Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D014771:Virion / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D010750:Phosphoproteins; D013462:Sulfur Radioisotopes; D015678:Viral Structural Proteins; D012967:Sodium Dodecyl Sulfate; D008715:Methionine