Germ-line contribution of embryonic stem cells in chimeric mice: influence of karyotype and in vitro differentiation ability.
The effect of the karyotype and the ability to differentiate in vitro upon germ-line transmission by A3-1 embryonic stem (ES) cells in chimeric mice were examined. Germ-line transmission was confirmed in ES cells exhibiting 38% and more of the normal karyotype, but no chimeric mice and/or germ-line transmitters were observed regardless of the karyotype when the cystic embryoid body (CEB) was formed on day 8 and later in the suspension culture. Germ-line transmission of the ES cells was not significantly influenced by formation of the simple embryoid body (SEB). Germ-line transmitters were preferentially observed in chimeras when the ES cell contribution to coat color was markedly increased, but this contribution to coat color varied regardless of the karyotype or in vitro differentiation ability. These results suggest that A3-1 ES cells which exhibit CEB at 7 days after suspension culture and approximately 40% of normal karyotype are capable of germ-line transmission in chimeric mice.
Suzuki
H
H
Laboratory of Molecular Genetics and Embryology, CSK Research Park Inc., Tokyo, Japan.
Kamada
N
N
Ueda
O
O
Jishage
K
K
Kurihara
Y
Y
Kurihara
H
H
Terauchi
Y
Y
Azuma
S
S
Kadowaki
T
T
Kodama
T
T
Yazaki
Y
Y
Toyoda
Y
Y
D000818:Animals; D002454:Cell Differentiation; D002678:Chimera; D004622:Embryo, Mammalian / Q000166:cytology*; D005307:Fertilization in Vitro; D005854:Germ Cells; D007621:Karyotyping; D051379:Mice; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000166:cytology*