Induction of hypoglycaemia in Japanese encephalitis virus infection: the role of T lymphocytes.
We report here development of hypoglycaemia in the convalescent phase of Japanese encephalitis virus (JEV) infection in mice by the induction of antigen-specific Ly1- 2+ T cells in the spleen which mediate hypoglycaemia through the generation of soluble T cell hypoglycaemic factor (TCHF). The TCHF acted in a dose-dependent manner and was found to be trypsin-sensitive and thermolabile. It was purified on Superose-12 high performance liquid chromatography (HPLC) gel filtration column and purified protein migrated as a approximately 25-kD band on SDS-PAGE. The JEV-induced hypoglycaemia coincided with an increased circulating glucagon level, without any alterations in blood insulin and growth hormone concentrations. These effects were mimicked by TCHF. These results indicate that JEV-primed T lymphocytes mediate hypoglycaemia through the production of a soluble hypoglycaemic factor.
Khanna
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N
Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
Mathur
A
A
Bharadwaj
M
M
Chaturvedi
U C
UC
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000627:therapeutic use; D000954:Antigens, Surface / Q000235:genetics; D001786:Blood Glucose / Q000032:analysis; D004672:Encephalitis, Japanese / Q000150:complications*; D005934:Glucagon / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism; D007003:Hypoglycemia / Q000209:etiology* / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D010641:Phenotype; D013154:Spleen / Q000737:chemistry / Q000502:physiology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology / Q000502:physiology
D000906:Antibodies; D000954:Antigens, Surface; D001786:Blood Glucose; D007328:Insulin; D010455:Peptides; D013006:Growth Hormone; D005934:Glucagon