Enhanced delivery of synthetic oligonucleotides to human leukaemic cells by liposomes and immunoliposomes.
The ability of pH-sensitive liposomes and immunoliposomes to deliver synthetic antisense oligonucleotides (oligos) into human myeloid and lymphoid leukaemia cells was examined. The cellular uptake of an 18mer anti-myb oligonucleotide encapsulated in liposomes was from three- to five-fold higher than that of 32P-oligos alone. In addition, anti-CD32 or anti-CD2 immunoliposomes improved the delivery of oligos to leukaemic cells carrying the appropriate receptor for the specific antibody-linked immunoliposome. The uptake of oligos was twice that of the liposome or non-specific immunoliposome encapsulated oligos. These findings support the use of liposomes or immunoliposomes to deliver antisense oligos into human leukaemic cells.
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Department of Haematology, Royal North Shore Hospital, St Leonards, NSW, Australia.
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D002784:Cholesterol; D004337:Drug Carriers; D005960:Glucosides; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018796:Immunoconjugates / Q000008:administration & dosage*; D007938:Leukemia / Q000473:pathology*; D008081:Liposomes; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D019301:Oleic Acid; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage* / Q000138:chemical synthesis; D010714:Phosphatidylethanolamines; D014407:Tumor Cells, Cultured
D004337:Drug Carriers; D005960:Glucosides; D018796:Immunoconjugates; D008081:Liposomes; D016376:Oligonucleotides, Antisense; D010714:Phosphatidylethanolamines; C018619:octyl-beta-D-glucoside; D019301:Oleic Acid; C020888:1,2-dielaidoylphosphatidylethanolamine; D002784:Cholesterol