title: Proviral organization and sequence analysis of feline immunodeficiency virus isolated from a Pallas' cat.
abstract: The nucleotide sequence and genomic organization have been determined for a highly cytopathic feline immunodeficiency virus (FIV) isolated from a Pallas' cat. The 9747-bp provirus of this virus, FIV-Oma, has typical lentivirus organization with LTRs, gag, pol, and env open reading frames (ORFs), putative vif and rev ORFs, and an ORF similar to ORF2/ORFA of domestic cat FIV isolates. Although the FIV-Oma provirus is 300 to 600 bp longer than other FIV proviruses, these additional bases are distributed throughout the genome. Phylogenetic analysis of a conserved region of the pol gene suggests that FIV-Oma is more closely related to some of the puma and lion lentiviruses than it is to domestic cat FIV isolates; however, many regions of the genome exhibit extensive nucleotide sequence divergence. None of the eight molecular proviral clones isolated from a genomic library are infectious, but we have constructed an infectious, cytopathic clone of FIV-Oma from subcloned and PCR-amplified fragments of these proviral clones. This clone will be useful for identifying the genetic determinants of FIV-Oma's biological activities.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Barr
authors.forename: L
authors.initials: L
authors.lastname: Zou
authors.forename: F
authors.initials: F
authors.lastname: Long
authors.forename: W A
authors.initials: WA
authors.lastname: Hoose
authors.forename: R J
authors.initials: RJ
authors.lastname: Avery
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D004279:DNA, Viral; D016679:Genome, Viral; D016089:Immunodeficiency Virus, Feline / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016086:Lentivirus / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011533:Proviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis*; D017423:Sequence Analysis, RNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral