Aminopeptidase A antiserum inhibits intracerebroventricular angiotensin II-induced dipsogenic and pressor responses.
Angiotensin II increases drinking and blood pressure when administered intracerebroventricularly. Intracerebroventricular injections of antiserum with anticatalytic activity against aminopeptidase A, the principal enzyme that metabolizes angiotensin II to angiotensin III, reduced the drinking and blood pressure responses to 10 pmol angiotensin II by 73% and 59%, respectively. APA antiserum had no effect on responses to angiotensin III administered intracerebroventricularly. A Glu-thiol inhibitor of aminopeptidase A also reduced angiotensin II-induced drinking. These results suggest that metabolism of angiotensin II to angiotensin III is an obligatory activation step for the brain angiotensin system.
Song
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Department of Pharmacology, Mount Sinai School of Medicine of the City University of New York, NY 10029, USA.
Wilk
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S
Healy
D P
DP
D000626:Aminopeptidases / Q000276:immunology*; D000804:Angiotensin II / Q000276:immunology / Q000494:pharmacology*; D000805:Angiotensin III / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D000918:Antibody Specificity; D001794:Blood Pressure / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D043384:Glutamyl Aminopeptidase; D007276:Injections, Intraventricular; D008297:Male; D008666:Metalloendopeptidases / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014662:Vasoconstrictor Agents / Q000276:immunology / Q000494:pharmacology*
D019138:Antibodies, Blocking; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D000805:Angiotensin III; D000626:Aminopeptidases; D043384:Glutamyl Aminopeptidase; D008666:Metalloendopeptidases