Volatile general anaesthetic actions on recombinant nACh alpha 7, 5-HT3 and chimeric nACh alpha 7-5-HT3 receptors expressed in Xenopus oocytes.
The effect of halothane and isoflurane was studied on the function of recombinant neurotransmitter receptors expressed in Xenopus oocytes. Both anaesthetics inhibited nicotinic acetylcholine type alpha 7 (nACh alpha 7) receptor-mediated responses, potentiated 5-hydroxytryptamine type 3 (5-HT3) receptor-mediated responses at low agonist concentrations, and inhibited the function of a chimeric receptor (with the N-terminal domain from the nACh alpha 7 receptor and the transmembrane and C-terminal domains from the 5-HT3 receptor) in a manner similar to that of the nACh alpha 7 receptor. Since the N-terminal domain of the chimeric receptor was from the nACh alpha 7 receptor, the observations suggest that the inhibition involves the N-terminal domain of the receptor.
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Laboratory of Molecular & Cellular Neurobiology, National Institute on Alcohol Abuse & Alcoholism, National Institutes of Health, Bethesda, MD 20892-8205, USA.
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Stewart
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Peoples
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D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D018680:Cholinergic Antagonists / Q000494:pharmacology*; D006221:Halothane / Q000494:pharmacology; D007530:Isoflurane / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000187:drug effects*; D011985:Receptors, Serotonin / Q000187:drug effects*; D011994:Recombinant Proteins / Q000378:metabolism; D017366:Serotonin Receptor Agonists / Q000494:pharmacology*; D014981:Xenopus
D018685:Anesthetics, Inhalation; D018680:Cholinergic Antagonists; D011950:Receptors, Cholinergic; D011985:Receptors, Serotonin; D011994:Recombinant Proteins; D017366:Serotonin Receptor Agonists; D007530:Isoflurane; D006221:Halothane