A gamma delta T cell specific surface receptor (WC1) signaling G0/G1 cell cycle arrest.
Three monoclonal antibodies (mAb; SC-6, SC-12, and SC-29) reactive with the gammadelta T cell-restricted antigen WC1 were obtained immunizing mice with an ovine interleukin (IL)-2-dependent gammadelta T cell line. These mAb strongly inhibited DNA synthesis in IL-2-dependent gammadelta T cell lines with cell cycle arrest in G0/G1 phase, but did not induce apoptosis. The mAb-induced growth arrest was reversible, either by removing the mAb or by co-culture with mitogen or anti-CD3 in the presence of IL-2. In contrast, addition of phorbol ester, ionomycin and IL-2 had no effect on the mAb-induced growth arrest. The observations define a biologically important role for the cell surface molecule WC1 in the regulation of gammadelta T cell proliferation and also provide a suitable system to study the relevant signal transduction events.
Takamatsu
H H
HH
Department of Immunology, Institute for Animal Health, Pirbright Laboratory, GB.
Kirkham
P A
PA
Parkhouse
R M
RM
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D002417:Cattle; D002453:Cell Cycle; D002478:Cells, Cultured; D005434:Flow Cytometry; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D012756:Sheep; D016176:T-Lymphocyte Subsets / Q000166:cytology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; C086500:DMBT1 protein, Bos taurus; D008562:Membrane Glycoproteins; D016692:Receptors, Antigen, T-Cell, gamma-delta