Approaches to high-volume screening assays of viral polymerases and related proteins.
Cole
J L
JL
Department of Antiviral Research, Merck Research Laboratories, West Point, Pennsylvania 19486, USA.
D001483:Base Sequence; D003124:Colorimetry / Q000379:methods; D004259:DNA-Directed DNA Polymerase / Q000032:analysis* / Q000378:metabolism; D019427:HIV Integrase / Q000032:analysis / Q000378:metabolism; D054303:HIV Reverse Transcriptase / Q000032:analysis* / Q000378:metabolism; D015497:HIV-1 / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000201:enzymology; D010761:Phosphorus Radioisotopes; D011865:Radioisotope Dilution Technique; D012190:Retroviridae / Q000201:enzymology*; D012588:Scintillation Counting / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods; D014158:Transcription, Genetic; D014316:Tritium; D014780:Viruses / Q000201:enzymology*
D010761:Phosphorus Radioisotopes; D014316:Tritium; D019427:HIV Integrase; D054303:HIV Reverse Transcriptase; D004259:DNA-Directed DNA Polymerase