title: [The detection of tramadol and its metabolites in urine by chromatographic methods].
abstract: The authors recommend detecting a compound drug tramadol and its metabolites in urinary extracts by thin-layer, gas-chromatographic, high-pressure liquid, and other chromatographic methods. The metabolites were identified with due consideration for their fragmentation during an electron strike thereof and their trimethylsilyl derivatives, mobility during separation in a thin layer, and results of selective extraction at various pH. The proposed scheme of biotransformation includes the formation of products of O- and N- demethylation, N, N- and N,O-didemethylation and hydroxylation of the cyclohexane fragment. The Rf metabolite values and their mass spectra are presented. During gas chromatographic analysis at an evaporator temperature at least 250 degrees C tramadol undergoes thermal destruction with the formation of water molecules and cyclohexane structure.
authors.forename: N V
authors.initials: NV
authors.lastname: Veselovskaia
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Kislun
authors.forename: S K
authors.initials: SK
authors.lastname: Eremin
authors.forename: B N
authors.initials: BN
authors.lastname: Izotov
authors.forename: A P
authors.initials: AP
authors.lastname: Viatchanina
authors.forename: O B
authors.initials: OB
authors.lastname: Dorogokupets
authors.forename: E B
authors.initials: EB
authors.lastname: Morozova
authors.forename: A E
authors.initials: AE
authors.lastname: Kovalenko
mesh_terms: D000701:Analgesics, Opioid / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods / Q000706:statistics & numerical data; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000706:statistics & numerical data; D002855:Chromatography, Thin Layer / Q000379:methods / Q000706:statistics & numerical data; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D014147:Tramadol / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000652:urine*
chemical_list: D000701:Analgesics, Opioid; D014147:Tramadol