title: Heterogeneity of hemoglobin A1d: assessment and partial characterization of two new minor hemoglobins, A1d3a and A1d3b, increased in uremic and diabetic patients, respectively.
abstract: We have separated and quantified two new minor hemoglobins named HbA1d3a and HbA1d3b. The level of HbA1d3a was significantly higher in uremic than in non-uremic patients (3.00 +/- 0.50% vs. 1.28 +/- 0.26% of total hemoglobin). It correlated well with carbamylated hemoglobin (r = 0.80, n = 81, p < 0.002) and with plasma urea concentration (r = 0.78, n = 81, p < 0.002). These data and the electrospray ionization mass spectrometric analysis provide strong evidence that HbA1d3a is an alpha-chain modified by carbamylation. The HbA1d3b level an diabetic patients was found to be 1.6-fold that in non-diabetic subjects (3.00 +/- 0.49 vs. 1.90 +/- 0.33). This was attributed to HbA1d3 modified by glycation. Indeed HbA1d3b correlated significantly with HbA1c (r = 0.71, p < 0.002) and with serum glucose level (r = 0.62, p < 0.002). These two new minor hemoglobins may serve as complements for the objective assessment of averaged long-term uremia and glycemia in uremic and diabetic patients.
authors.affiliation: Department of Clinical Chemistry, University Hospital, Albert-Ludwigs-Universität, Freiburg, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Bissé
authors.forename: P
authors.initials: P
authors.lastname: Huaman-Guillen
authors.forename: P
authors.initials: P
authors.lastname: Hörth
authors.forename: A
authors.initials: A
authors.lastname: Busse-Grawitz
authors.forename: M
authors.initials: M
authors.lastname: Lizama
authors.forename: A
authors.initials: A
authors.lastname: Krämer-Guth
authors.forename: W
authors.initials: W
authors.lastname: Haehnel
authors.forename: H
authors.initials: H
authors.lastname: Wieland
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003920:Diabetes Mellitus / Q000097:blood*; D018740:Genetic Heterogeneity; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000235:genetics; D006801:Humans; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014511:Uremia / Q000097:blood*
chemical_list: D006442:Glycated Hemoglobin; C093254:hemoglobin A1d