title: Anaphylactic IgE and IgG1 production for hapten can be enhanced by contrast media.
abstract: Various side effects have been associated with the clinical use of contrast media. Immunological mechanisms have been proposed but there have been very few experimental studies with animal models. We have attempted to develop murine models to determine whether or not anaphylactic antibodies such as IgE and IgG1 against hapten (DNP) were enhanced with contrast medium (iopamidol) as an adjuvant or if the contrast medium itself produced antibodies of the IgE class. The results showed that anti-hapten IgE and IgG1 production was greatly enhanced with immunogen plus contrast medium. Anti-contrast medium antibodies of the IgE class could not be detected by PCA reactions. The enhancement of IgE and IgG1 production for hapten was associated with IL-4 release by the neutralization test used by monoclonal anti-IL-4 antibodies. This is the first observation to show that contrast media may have a strong adjuvant effect for the production of IgE and IgG1. This murine model demonstrates a possible immunological function of contrast media in vivo.
authors.affiliation: Department of Radiology, Juntendo University, School of Medicine, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyauchi
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.forename: C
authors.initials: C
authors.lastname: Ushiyama
authors.forename: Z
authors.initials: Z
authors.lastname: Ovary
authors.forename: K
authors.initials: K
authors.lastname: Okumura
authors.forename: H
authors.initials: H
authors.lastname: Katayama
mesh_terms: D000707:Anaphylaxis / Q000145:classification / Q000276:immunology*; D000818:Animals; D003287:Contrast Media / Q000009:adverse effects; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006433:Hemocyanins / Q000009:adverse effects / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D007479:Iopamidol / Q000009:adverse effects; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology
chemical_list: C042158:2,4-dinitrophenyl keyhole limpet hemocyanin; D003287:Contrast Media; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D006433:Hemocyanins; D007479:Iopamidol