Expression and induction by IL-6 of the normal and variant genes for human plasminogen.
We examined the promoter activity of the gene for human plasminogen (PLG) employing its 1.1 kb fragment of the 5'-flanking region inserted in front of a reporter gene. Deletion analysis revealed that a region surrounding the transcription start site was essential for the PLG expression. Since the PLG gene has three sequences for the interleukin-6 (IL-6) responsive element, we examined the effect of IL-6 on the PLG expression. IL-6 stimulation of PLG resulted in a 2.5-fold increase in its transcription. This is also true for the PLG gene of a case with dysplasminogenemia. Although the patient's gene had six mutations in the 5'-flanking region, its promoter activity was 1.8-fold that of normal PLG.
Kida
M
M
Department of Molecular Pathological Biochemistry, Yamagata University School of Medicine, Japan.
Wakabayashi
S
S
Ichinose
A
A
D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D005347:Fibroblasts; D015723:Gene Library; D017930:Genes, Reporter; D014644:Genetic Variation; D005822:Genetic Vectors; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D007700:Kinetics; D008113:Liver Neoplasms; D010958:Plasminogen / Q000096:biosynthesis* / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis
D015850:Interleukin-6; D011994:Recombinant Proteins; D010958:Plasminogen; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase