title: [Autologous keratinocyte culture on hyaluronic acid ester membranes: an alternative in complicated wound management?].
abstract: Cultivation and transplantation of autologous keratinocytes has been used in the last 15 years to treat complicated wounds of different origin. In spite of excellent technical advancements and clinical experiences cultured keratinocyte grafting still is associated with practical limitations. Application of hyaluronic acid ester membranes as carrier substrate for the transfer of keratinocytes allows improved graft handling: reduces total time required for tissue cultivation and furthermore enhances vitality of the keratinocytes because of possible grafting at semiconfluence.
authors.affiliation: Zentrum der Chirurgie-Klinik für Unfallchirurgie, Klinikum der Johann-Wolfgang-Goethe-Universität, Frankfurt a. M.
authors.forename: D
authors.initials: D
authors.lastname: Hollander
authors.forename: M
authors.initials: M
authors.lastname: Stein
authors.forename: A
authors.initials: A
authors.lastname: Bernd
authors.forename: J
authors.initials: J
authors.lastname: Windolf
authors.forename: R
authors.initials: R
authors.lastname: Wagner
authors.forename: A
authors.initials: A
authors.lastname: Pannike
mesh_terms: D000368:Aged; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D004952:Esters; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid; D015603:Keratinocytes / Q000637:transplantation*; D007871:Leg Ulcer / Q000628:therapy*; D014182:Transplantation, Autologous; D014947:Wounds and Injuries / Q000628:therapy*
chemical_list: D003470:Culture Media; D004952:Esters; D006820:Hyaluronic Acid