title: Effect of ethylene thiourea on cultured rat embryos in the presence of hepatic microsomal fraction.
abstract: To investigate whether teratogenicity of ethylene thiourea (ETU) is due to ETU per se or to its metabolites, rat embryos cultured in vitro were exposed to 10 and 30 micrograms/ml of ETU in the presence or absence of rat liver microsomal fraction (S9mix). In the absence of S9mix, the incidence of morphological abnormalities increased dose-dependently. In the presence of S9mix, abnormal morphogenesis was almost absent in all embryos. These findings suggest that teratogenicity of ETU is due to ETU per se.
authors.affiliation: Department of Anatomy II, Azabu University, School of Veterinary Medicine, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iwase
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Shirai
authors.forename: F
authors.initials: F
authors.lastname: Akahori
authors.forename: T
authors.initials: T
authors.lastname: Masaoka
authors.forename: T
authors.initials: T
authors.lastname: Takizawa
authors.forename: K
authors.initials: K
authors.lastname: Arishima
authors.forename: Y
authors.initials: Y
authors.lastname: Eguchi
mesh_terms: D000014:Abnormalities, Drug-Induced; D000818:Animals; D001711:Biotransformation; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D008862:Microsomes, Liver / Q000378:metabolism*; D009024:Morphogenesis / Q000187:drug effects; D009924:Organ Culture Techniques; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013723:Teratogens / Q000493:pharmacokinetics / Q000633:toxicity*; D013890:Thiourea / Q000493:pharmacokinetics* / Q000633:toxicity*
chemical_list: D013723:Teratogens; D013890:Thiourea