A simple method to design PCR primer to detect genomic DNA of parasites and its application to Dirofilaria immitis.
A simple method to design a polymerase chain reaction (PCR) primer for parasites of interest was developed using Dirofilaria immitis as a test sample. The method involved the cloning and sequencing of randomly amplified DNA of the parasite, and designing a primer based on the resulting DNA sequence. Using the primer, DNA fragments of the expected length were amplified by a regular PCR with genomic DNA of Dirofilaria immitis.
Nagano
I
I
Department of Parasitology, Gifu University School of Medicine, Japan.
Zhiliang
W
W
Nakayama
M
M
Takahashi
Y
Y
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000096:biosynthesis; D018509:DNA, Helminth / Q000032:analysis*; D004183:Dirofilaria immitis / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D018509:DNA, Helminth