Significant suppression of rat liver aldolase B by a toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl.
A toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of liver aldolase B in rats. Hepatic aldolase activity in PenCB-treated rats was significantly reduced to about 50% of that in free- and pair-fed control groups. The reduced aldolase activity following PenCB-treatment was due to the marked suppression of the expression of aldolase B shown by immunoblot analysis after SDS-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. The suppression of rat liver aldolase B could be a key biochemical lesion caused by PenCB.
Ishii
Y
Y
Faculty of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan.
Kato
H
H
Hatsumura
M
M
Ishida
T
T
Ariyoshi
N
N
Oguri
K
K
D000818:Animals; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000187:drug effects* / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D011506:Proteins; D011078:Polychlorinated Biphenyls; D005634:Fructose-Bisphosphate Aldolase; C023035:3,4,5,3',4'-pentachlorobiphenyl