title: [Conformation properties of heme-containing proteins using spin labels].
abstract: The review briefly summarizes the results of spin-label studies of conformational transitions in monomeric globins (myoglobin, leghemoglobin, erythrocruorin) as well as in another heme-containing protein, cytochrome c, induced by heme ligands and pH. In contrast with integral methods of investigation, for proteins with a known spatial structure the use of the spin-labelling technique makes it possible to obtain information on the conformational behaviour of the isolated parts of the protein structure which, alongside with the results obtained by other physico-chemical methods, allows a conclusion on the nature of conformational movements in the protein during its functioning. The experimental results fit well into the model which represents a Mb-like structure as composed of three independent rigid helical fragments: AE(ABCDE), F and GH, whose reciprocal arrangement is controlled by N- and C-terminal salt bridges. Synchronous displacement of the above fragments relative to one another, which is due either to the ligand attachment and structural changes in the heme complex or to disturbances in ionic interactions at the N- and/or C-end(s) of the structure under effect of pH or allosteric effectors, might serve as a structural basis for homo- and heterotropic regulation in hemoglobin. Similarly, the spin label method allows for tracing and obtaining important information about local conformational events in cytochrome c which in the native protein are associated with a change in pH in the range of 5-13 and are accompanied by the substitution of the heme sixth ligand, Met-80, by Lys-79 (pH 9.3), and then by Tyr-67 (pK 11.1). Local changes in the conformation and dynamic properties of native cytochrome c provide, if necessary, global changes in its structure upon alkaline denaturation. It was found that substitution of the heme protein ligand, Met-80, by the external ligand, cyanide, markedly alters the dynamic properties of the polypeptide chain segment 67-75 adjacent to Met-80.
authors.forename: G B
authors.initials: GB
authors.lastname: Postnikova
mesh_terms: D006420:Hemeproteins / Q000737:chemistry*; D011487:Protein Conformation; D013113:Spin Labels
chemical_list: D006420:Hemeproteins; D013113:Spin Labels