title: Characterization of the diversity and the transferrin-binding domain of gonococcal transferrin-binding protein 2.
abstract: The molecular weight heterogeneities of Tbp1 and Tbp2 among a panel of 45 gonococcal isolates were assessed. The tbpB genes from four of these strains were sequenced to characterize the Tbp2 sequence diversity among gonococci. By expressing truncated versions of gonococcal Tbp2, we delimited the extent of Tbp2 necessary for transferrin binding in a Western blot.
authors.affiliation: Department of Medicine, School of Medicine, University of North Carolina at Chapel Hill 27599, USA. cornelis@med.unc.edu
authors.forename: C N
authors.initials: CN
authors.lastname: Cornelissen
authors.forename: J E
authors.initials: JE
authors.lastname: Anderson
authors.forename: P F
authors.initials: PF
authors.lastname: Sparling
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D033862:Iron-Binding Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D011990:Receptors, Transferrin / Q000737:chemistry* / Q000235:genetics; D017421:Sequence Analysis; D014168:Transferrin / Q000378:metabolism*; D033882:Transferrin-Binding Protein B; D033863:Transferrin-Binding Proteins
chemical_list: D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D011990:Receptors, Transferrin; D014168:Transferrin; D033882:Transferrin-Binding Protein B; D033863:Transferrin-Binding Proteins