title: Nucleolar organizer regions in a model of cell hyperactivity and regression.
abstract: Nucleolar organizer regions stained with colloidal silver techniques (AgNOR) evidence sites of active rRNA transcription. It has been proved that AgNOR undergo a rise in number and variations in size and shape in conditions which traditionally involve enhanced cell proliferation and rRNA transcription. AgNOR have been described as a marker of malignant transformation in multiple entities. Our laboratory has previously described their value as markers of radioinduced damage. The finding, at light microscopy level, that silver staining persisted at later post-irradiation times when cells are characteristically inactive, prompted the present study to correlate findings at light microscopy level with the ultrastructural analysis of nucleoli and their AgNOR in a model of irradiated skin. We herein attempt to explain the biological significance of AgNOR variations in the different phases of radioinduced response (which involves cellular hyperactivity followed by regressive features). Ten Wistar rats were submitted to local irradiation of the left leg (the shielded right leg was used as control) with 50 Gy x rays and killed 15 days post- irradiation. Silver staining was performed on ultrathin sections. In the basal layer of control epithelium silver affinity was established for fibrillar centers (FC) and fibrillar dense components (DFC). During the phase of radioinduced hyperplasia (1-3 days post-exposure) basal cells exhibit large reticular nucleoli, with irregular contours and silver staining on DFC. In the regressive phase (4-5 days post-irradiation) silver staining persists despite the halt in transcriptional activity, associated to homogeneous and compact nucleoli. These findings suggest caution in the interpretation of silver staining patterns.
authors.affiliation: Dept. of Radiobiology, National Atomic Energy Commission, Capital Federal, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Morales
authors.forename: A E
authors.initials: AE
authors.lastname: Schwint
authors.forename: M E
authors.initials: ME
authors.lastname: Itoiz
mesh_terms: D000818:Animals; D002455:Cell Division; D003102:Colloids; D005528:Foot / Q000473:pathology / Q000528:radiation effects; D006965:Hyperplasia; D015603:Keratinocytes / Q000528:radiation effects / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D011833:Radiation Injuries, Experimental / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D016622:Silver Staining; D014158:Transcription, Genetic
chemical_list: D003102:Colloids; D012335:RNA, Ribosomal