title: Anti-glomerular basement membrane antibody-induced inflammation in rat cochlear plexus.
abstract: Autoimmune inner ear diseases have been recognized recently, but the mechanism of hearing loss is still unclear. This study was aimed to induce immunological inflammation in cochlea to establish a model and to examine an effect of prednisolone on the induction. Lewis rats were immunized with normal rabbit IgG in complete Freund's adjuvant, and then injected intravenously with rabbit anti-rat glomerular basement membrane (GBM) antibody twice (anti-GBM Ab group). The binding of anti-GBM antibody and inflammatory cell infiltration in the cochlear plexus were investigated using immunofluorescence microscopy. Immunofluorescence microscopic examination revealed specific binding of anti-GBM Ab to basement membrane of capillaries in the cochlear plexus. Compared to normal rats injected with saline, larger numbers of infiltrated CD4-positive T cells (p < 0.01) and monocytes/macrophages (p < 0.01) were observed. Furthermore, prednisolone suppressed the infiltration of CD4-positive cells (p < 0.05) and monocytes/macrophages (p < 0.01). These results suggest that an inflammatory reaction was induced by binding of anti-basement membrane antibody to cochlear capillaries in inner ear and that this inflammation in cochlea could be reduced by prednisolone.
authors.affiliation: Department of Otolaryngology, Niigata University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Satoh
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D000906:Antibodies / Q000276:immunology*; D001323:Autoantibodies; D001485:Basement Membrane / Q000276:immunology*; D001665:Binding Sites; D003051:Cochlea / Q000276:immunology*; D005260:Female; D007249:Inflammation / Q000188:drug therapy / Q000209:etiology* / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D011239:Prednisolone / Q000627:therapeutic use; D011817:Rabbits; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D000906:Antibodies; D001323:Autoantibodies; C051951:antiglomerular basement membrane antibody; D011239:Prednisolone