Molecular cloning of a putative serotonin receptor gene from barnacle, Balanus amphitrite.
We isolated a putative serotonin receptor gene from a genomic library of the barnacle, balanus amphitrite Darwin, using an Ncol fragment of the barnacle G protein-coupled receptor gene that is homologous to the alpha 2-adrenoceptor. The cloned genomic DNA had no intron and specified an open reading frame of 1137 base pairs encoding 379 amino acids (aa). The predicted aa sequence has a typical seven hydrophobic transmembrane spanning region and a consensus G protein-binding motif. This receptor was most homologous to the human 5HT1A receptor and closely related to other 5HT1 receptor subtypes.
Kawahara
H
H
Marine Biotechnology Institute Co. Ltd., Shimizu Laboratory, Japan.
Isoai
A
A
Shizuri
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011985:Receptors, Serotonin / Q000235:genetics* / Q000378:metabolism; D044263:Receptors, Serotonin, 5-HT1; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D001468:Thoracica / Q000145:classification / Q000235:genetics*
D011985:Receptors, Serotonin; D044263:Receptors, Serotonin, 5-HT1; D004247:DNA; D019204:GTP-Binding Proteins