Immunoreactive cytokines within primates.
Peripheral blood mononuclear cells of primates (man, orang utan, gorilla, baboon), rodents (mouse, rat), carnivores (cat, dog), artiodactyls (cattle, goat, pig) and perissodactyls (horse) were isolated and stimulated with mitogens (5 micrograms/ml LPS, 5 micrograms/ml PHA) at 37 degrees C. Cytokines immunoreactive to monoclonal antibodies (mAb) directed to human cytokines (TNF-alpha, IL-1 alpha, IL-2, IL-6, IFN-gamma) could be detected by enzyme-linked immunosorbent assay (ELISA) in the case of primates only. The mAb used did not recognize cytokines of the other mammalian species investigated. The results demonstrate the close relationship within the primates from the immunophysiological point of view.
Ahne
W
W
Institute of Zoology, Fishery Biology and Fish Diseases, Faculty of Veterinary Medicine, University of Munich, Germany.
Mayr
A
A
Wiesner
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D002415:Cats; D002417:Cattle; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006041:Goats; D006071:Gorilla gorilla; D006736:Horses; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D010215:Papio; D010802:Phylogeny; D010835:Phytohemagglutinins / Q000494:pharmacology; D011148:Pongo pygmaeus; D011323:Primates / Q000276:immunology* / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D013552:Swine
D000911:Antibodies, Monoclonal; D016207:Cytokines; D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins