An enzyme immunoassay for prostaglandin E2 using biotin-prostaglandin B2 conjugate as a tracer.
Prostaglandin E2 (PGE2) was converted into prostaglandin B2 (PGB2) by alkaline treatment and quantitated by a novel and specific competitive enzyme immunoassay using an anti-PGB2 antibody and a biotin-PGB2 conjugate as a tracer. This assay was relatively specific for PGE1 and PGE2 (n-6 type); the reactivity for PGE3 (n-3 type) was below 2% of that for n-6 type, and was applicable to the quantitation of PGE2 synthesized in lipopolysaccharide-stimulated peritoneal macrophages from mice fed either a high linoleate or a high alpha-linolenate diet.
Watanabe
S
S
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Japan.
Yoshida
C
C
Kobayashi
T
T
Okuyama
H
H
D000818:Animals; D001710:Biotin; D015232:Dinoprostone / Q000032:analysis*; D007124:Immunoenzyme Techniques; D051379:Mice; D011456:Prostaglandins B / Q000032:analysis*; D011817:Rabbits
D011456:Prostaglandins B; D001710:Biotin; C042026:prostaglandin B2; D015232:Dinoprostone