Helicobacter pylori antibody titres in serum, plasma and successively thawed specimens: implications for epidemiological and clinical studies.
Agreement between Helicobacter pylori IgG antibodies measured using the Pylori-set EIA-G kit in serum, plasma and successively thawed specimens was studied and the implications for epidemiological and clinical studies assessed. Plasma titres may differ from serum titres by -6% to +8% and therefore may be substituted for serum. The change in titre around the cut off value was -0.31 (se = 5.7, p = 0.96) per thaw. The estimated maximum drop after three thawings, 34.5, would result in only a small decrease in sensitivity (1.3%). For qualitative epidemiological studies, this additional misclassification rate is relatively small. However, positive titres did reduce over successive thawings, with the estimated maximum drop being 11.4% per thaw. Therefore, thawing does need to be considered as a contributing factor when interpreting titre drops in eradication trials. Baseline and follow up specimens from clinical studies should be thawed once only and tested concurrently.
Pearce
D C
DC
Department of Public Health and Community Medicine, Ballarat Base Hospital, University of Melbourne.
Peach
H G
HG
Farish
S J
SJ
D000907:Antibodies, Bacterial / Q000097:blood*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D015203:Reproducibility of Results; D013048:Specimen Handling
D000907:Antibodies, Bacterial