GAK: a cyclin G associated kinase contains a tensin/auxilin-like domain.
We have cloned a cDNA encoding a novel association partner of cyclin G by West-Western blotting. The cDNA encodes a protein that harbors a Ser/Thr protein kinase-like catalytic domain at the N-terminal. Hence, we named it GAK (cyclin G-associated kinase). The long C-terminal extension shares homology with tensin and auxilin, and contains a leucine zipper region. Co-immunoprecipitation and Western blotting showed that GAK and cyclin G associate together in vivo. GAK also co-precipitated with CDK5, and CDK5 was found to be associated with cyclin G. We also showed by BIAcore analysis that the GAK-cyclin G interaction was direct.
Kanaoka
Y
Y
Department of Molecular Genetics, Osaka University, Yamadaoka, Suita, Japan.
Kimura
S H
SH
Okazaki
I
I
Ikeda
M
M
Nojima
H
H
D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003001:Cloning, Molecular; D056746:Cyclin G; D056766:Cyclin G1; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D007700:Kinetics; D008840:Microfilament Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry; D011233:Precipitin Tests; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D000070876:Tensins
D033942:Adaptor Proteins, Vesicular Transport; D000911:Antibodies, Monoclonal; C534704:Ccng1 protein, rat; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins; D018076:DNA, Complementary; C103904:Gak protein, rat; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D000070876:Tensins; C000603372:Tns1 protein, rat; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases