Gene identification in 1.6-Mb region of the Down syndrome region on chromosome 21.
The Down syndrome (DS) region has been defined by analyses of partial trisomy 21. The 2.5-Mb region between D21S17 and ERG is reportedly responsible for the main features of DS. Within this 2.5-Mb region, we focused previously on a distal 1.6-Mb region from an analysis of Japanese DS patients with partial trisomy 21. Previously we also performed exon-trapping and direct cDNA library screening of a fetal brain cDNA library and identified a novel gene TPRD. Further screening of a fetal heart cDNA library was performed and a total of 44 possible exons and 97 cDNA clones were obtained and mapped on a BamH1 map. By rescreening other cDNA libraries and a RACE reaction, we isolated nearly full-length cDNAs of three additional genes [holocarboxylase synthetase (HCS), G protein-coupled inward rectifier potassium channel 2 (GIRK2), and a human homolog of Drosophila minibrain gene (MNB)] and a coding sequence of a novel inward rectifier potassium channel-like gene (IRKK). The gene distribution and direction of transcription were determined by mapping both ends of the cDNA sequences. We found that these genes, except IRKK, are expressed ubiquitously and are relatively large, extending from 100 kb to 300 kb on the genome. These nearly full-length cDNA sequences should facilitate understanding of the detailed genome structure of the DS region and help to elucidate their role in the etiology of DS.
Ohira
M
M
Laboratory of Gene Structure 1, Kazusa DNA Research Institute, Chiba, Japan. oohira@kazusa.or.jp
Seki
N
N
Nagase
T
T
Suzuki
E
E
Nomura
N
N
Ohara
O
O
Hattori
M
M
Sakaki
Y
Y
Eki
T
T
Murakami
Y
Y
Saito
T
T
Ichikawa
H
H
Ohki
M
M
D000595:Amino Acid Sequence; D001921:Brain / Q000378:metabolism; D019731:Carbon-Nitrogen Ligases; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004314:Down Syndrome / Q000235:genetics*; D005318:Fetal Heart / Q000378:metabolism; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D006801:Humans; D008025:Ligases / Q000235:genetics; D008969:Molecular Sequence Data; D015221:Potassium Channels / Q000235:genetics; D024661:Potassium Channels, Inwardly Rectifying; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011505:Protein-Tyrosine Kinases; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D000097603:Dyrk Kinases
D019731:Carbon-Nitrogen Ligases; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D008025:Ligases; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases; D000097603:Dyrk Kinases; C023312:holocarboxylase synthetases