title: Enhancing effect of non-ionic surfactant on the inactivation of lipopolysaccharide by steam-heat treatment I.
abstract: Polyoxyethylene (20) sorbitan mono-fatty acid esters strongly enhanced the inactivation of lipopolysaccharide (LPS) by steam-heat treatment at 121 degrees C, as assayed by using the Limulus amebocyte lysate (LAL) and the pyrogen test. In an aqueous solution containing 0.1% surfactant, the decrease of LPS (1 microgram/ml) from E. coli 055:B5 at 121 degrees C followed first-order kinetics. Based on the LAL assay, 0.1% surfactant was essential to achieve 3-log cycle reduction of LPS with concomitant loss of pyrogenicity by steam-heat treatment for 20 min at 121 degrees C. Steam-heat treatment for 20 min at 121 degrees C in the absence of surfactant was insufficient to achieve depyrogenation. Polyoxyethylene (9) lauryl ether and decaglycerin mono-laurate similarly enhanced depyrogenation by steam-heat treatment. The effects of all the surfactants were concentration-dependent for all of the six kinds of LPS examined.
authors.affiliation: Pharmaceutical Research Center, Shiseido Company, Yokohama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Bamba
authors.forename: R
authors.initials: R
authors.lastname: Matsui
authors.forename: K
authors.initials: K
authors.lastname: Watabe
mesh_terms: D005240:Feasibility Studies; D005334:Fever / Q000209:etiology / Q000517:prevention & control; D006358:Hot Temperature; D006801:Humans; D007263:Infusions, Parenteral / Q000009:adverse effects; D008070:Lipopolysaccharides / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D008070:Lipopolysaccharides; D013501:Surface-Active Agents