Role of bcl-2 in the development of lymphoid cells from the hematopoietic stem cell.
To investigate the role of bcl-2 in lymphohematopoiesis, a long-term bone marrow reconstitution system was established. Transplantation of 1,000 c-Kit+ Sca-1+ and lineage markers negative cells from bcl-2-l-mouse bone marrow resulted in long-term reconstitution of nonlymphoid cells. However, T cells were totally absent and B-lymphocyte development was severely impaired at a very early stage of differentiation in the chimeric mouse. On the other hand, transplantation of day 14 fetal liver cells from bcl-2-l-mice resulted in generation of both T and B cells in the recipient, albeit transiently. These data suggest that bcl-2 plays a critical role in the development of lymphoid progenitor cells from the hematopoietic stem cell (HSC), but is not essential for the development of nonlymphoid cells and the self-renewal of HSC. In addition, lymphopoiesis from fetal liver HSC appears to be less dependent on bcl-2 than adult bone marrow HSC.
Matsuzaki
Y
Y
Department of Immunology, University of Tsukuba, Ibaraki, Japan.
Nakayama
K
K
Nakayama
K
K
Tomita
T
T
Isoda
M
M
Loh
D Y
DY
Nakauchi
H
H
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D006084:Graft Rejection / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D007156:Immunologic Memory / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects
D019253:Proto-Oncogene Proteins c-bcl-2