Ethanol in utero induces epithelial cell damage and altered kinetics in the developing rat intestine.
The effect of prenatal ethanol exposure on the intestinal maturation of rat fetuses was investigated to understand the nutritional alterations found in the offspring of alcoholic mothers. Female Wistar rats were maintained on solid diet and 25% ethanol solution as drinking fluid during pregnancy, and non-alcoholic isocaloric pregnant mothers were used as controls. At birth, intestines from unsuckled pups were removed for study. The weight and length of the intestine decreased significantly when ethanol was present in utero. Ultrastructural evaluation of the epithelium revealed loss of contact between neighboring enterocytes and abnormal dilation of the cisternae of the Golgi apparatus in ethanol-exposed pups. Further, increased lysosome-like vesiculation and enhanced lysosomal beta-galactosidase activity was observed in these neonates. The total number of absorptive enterocytes in the epithelium was reduced by 30% in ethanol-exposed neonates as compared to controls, due to altered cell growth and death during fetal life. Ethanol in utero stimulated epithelial cell migration which compensated cell loss, as demonstrated by 5'-Bromodeoxyuridine labeling. These findings could have important implications for the assimilation of nutrients and failure to thrive in infants with fetal alcohol syndrome.
Estrada
G
G
Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Spain.
Del Rio
J A
JA
García-Valero
J
J
López-Tejero
M D
MD
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000196:embryology / Q000473:pathology; D008247:Lysosomes / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D001616:beta-Galactosidase / Q000378:metabolism
D000431:Ethanol; D001616:beta-Galactosidase