Primary structure of EPV20, a secretory glycoprotein containing a previously uncharacterized type of domain.
A 20-kDa glycoprotein, EPV20, was isolated from bovine milk and characterized. The primary structure was determined by cDNA and protein sequencing combined with mass spectrometry. EPV20 is a 130-residue polypeptide synthesized with a 19-residue signal peptide. The function of EPV20 is unknown, but it displays 79% sequence similarity to a putative protein deduced from a human testis cDNA sequence designated HE1 (human epididymis clone 1) (Kirchhoff, C., 1992. EMBL/GeneBank/DDBJ Databases, accession number X67698). Northern blot analysis showed the bovine EPV20 to be expressed in kidney, spleen, liver and mammary gland, but remarkably not in bovine testis. The six Cys residues of EPV20 were found to be disulfide-linked in a 1-6, 2-3 and 4 5 pattern. This disulfide arrangement has been observed in other proteins, e.g. in human prostatic acid phosphatase, but the spacing between the cystines differs. Therefore, EPV20 represents a new structure among the large group of proteins containing domains with three disulfide bonds.
Larsen
L B
LB
Protein Chemistry Laboratory, University of Aarhus, Denmark.
Ravn
P
P
Boisen
A
A
Berglund
L
L
Petersen
T E
TE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins; D002417:Cattle; D018076:DNA, Complementary / Q000235:genetics; D004220:Disulfides / Q000737:chemistry; D015870:Gene Expression; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D008894:Milk Proteins / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012333:RNA, Messenger / Q000235:genetics; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D018076:DNA, Complementary; D004220:Disulfides; D006023:Glycoproteins; D008894:Milk Proteins; C104585:NPC2 protein, human; D012333:RNA, Messenger; D033921:Vesicular Transport Proteins