title: Inducible expression of a Porphyromonas gingivalis W83 membrane-associated protease.
abstract: The Tpr protease of Porphyromonas gingivalis W83 is a membrane-associated enzyme capable of hydrolyzing a chromogenic bacterial collagenase substrate. An isogenic mutant lacking a functional tpr gene had a greatly reduced ability to hydrolyze the collagenase substrate. Activity was restored to the tpr mutant by introducing a shuttle plasmid containing the tpr gene. Expression of the gene is induced by nutrient limitation, as shown by enzymatic and Northern analyses.
authors.affiliation: Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Park
authors.forename: B
authors.initials: B
authors.lastname: Lu
authors.forename: C
authors.initials: C
authors.lastname: Mazur
authors.forename: B C
authors.initials: BC
authors.lastname: McBride
mesh_terms: D017364:Collagenases / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000235:genetics*; D008666:Metalloendopeptidases / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000201:enzymology*
chemical_list: D008565:Membrane Proteins; D010450:Endopeptidases; D017364:Collagenases; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase