Inhalation of diesel exhaust enhances antigen-specific IgE antibody production in mice.
To examine the effects of diesel exhaust (DE) inhalation on IgE antibody production, BALB/c mice were exposed to 0 (control), 3.0 and 6.0 mg/m3 DE inhalation for 3 weeks. Intranasal sensitization with ovalbumin (OA) three times at intervals of 3 weeks was conducted immediately before, immediately after and 3 weeks after DE inhalation. Body weight and thymus weight for the DE-exposed and control mice were essentially the same but spleen weight in mice exposed to 6 mg/m3 significantly increased. Anti-OA IgE antibody titers in the sera of mice exposed to 6 mg/m3 was significantly higher than the control. Total IgE and anti-OA IgG in sera for DE-exposed and control mice remained basically the same. To investigate cytokine production in mice exposed to 6 mg/m3, spleen cells from DE-exposed and control mice were stimulated with OA in vitro and cytokine production in the culture supernatants was measured by ELISA. In vitro antigen-stimulated interleukin-4 (IL-4) and -10 (IL-10) production in spleen cells of exposed mice significantly increased compared to the control. In vitro interferon (IFN)-gamma production in spleen cells of exposed mice markedly decreased. DE inhalation is thus shown to have adverse effect on antigen-specific IgE antibody production in mice through alteration of the cytokine network.
Fujimaki
H
H
National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki, Japan.
Saneyoshi
K
K
Shiraishi
F
F
Imai
T
T
Endo
T
T
D000280:Administration, Inhalation; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D000939:Epitopes / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D013154:Spleen / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism; D013950:Thymus Gland / Q000033:anatomy & histology / Q000187:drug effects; D001335:Vehicle Emissions / Q000633:toxicity*
D000939:Epitopes; D001335:Vehicle Emissions; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin