[Role of intercellular adhesion molecule (ICAM)-1 in the interaction between glomerular endothelial cells and granulocytes].
The present study was designed to investigate the role of intercellular adhesion molecule (ICAM)-1 in the interaction between glomerular endothelial cells (GEN) and granulocytes. Expression of ICAM-1 was promoted after stimulation with tumor necrosis factor (TNF)-alpha. This effect was dose- and time-dependent. Granulocytes attached to GEN in accordance with the increased expression of ICAM-1 on the cells stimulated with TNF-alpha. In addition, the adhesion of granulocytes to activated GEN with TNF-alpha stimulated specific [51Cr] release from the monolayers. Furthermore, monoclonal antibodies to TNF-alpha dose-dependently inhibited the adhesion of granulocytes to activated GEN. In summary, ICAM-1 is related to the glomerular endothelial cell injury induced by granulocytes activated with TNF-alpha, suggesting that ICAM-1 may play a role in the initiation of certain types of glomerulonephritis with granulocyte infiltration.
Miwa
N
N
Department of Medicine, Tokyo Women's Medical College, Japan.
Nitta
K
K
Yumura
W
W
Nihei
H
H
D000818:Animals; D002417:Cattle; D002450:Cell Communication / Q000502:physiology*; D004727:Endothelium / Q000166:cytology; D006098:Granulocytes / Q000166:cytology*; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D007678:Kidney Glomerulus / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1