title: A putative ABC-transport operon of Mycobacterium smegmatis.
abstract: We have recently described the mpr gene of Mycobacterium smegmatis whose product confers resistance to mycobacteriophages L5 and D29 when overproduced (Barsom and Hatfull (1996) Mol. Microbiol. 21, 159-170). We have determined the nt sequence of approximately 3.5 kb immediately adjacent to mpr which appears to encode components of an ATP-binding cassette (ABC) transport system. Four closely-spaced open reading frames (ORF) were identified although two of these may cooperate to produce an integral membrane component of the transport system via a programmed translational frameshift. Another putative protein is also predicted to be an integral membrane protein, while the third is an ABC-transporter protein. We propose that these three putative proteins form a mycobacterial membrane-bound complex involved in protein-dependent transport. This is the first ABC-transport system to be described in mycobacteria.
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, PA 15260, USA.
authors.forename: E K
authors.initials: EK
authors.lastname: Barsom
authors.forename: G F
authors.initials: GF
authors.lastname: Hatfull
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D002473:Cell Wall / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018965:Frameshifting, Ribosomal / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics*; D016366:Open Reading Frames; D009876:Operon; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D026901:Membrane Transport Proteins