title: Crystal structure of colicin Ia.
abstract: The ion-channel forming colicins A, B, E1, Ia, Ib and N all kill bacterial cells selectively by co-opting bacterial active-transport pathways and forming voltage-gated ion conducting channels across the plasma membrane of the target bacterium. The crystal structure of colicin Ia reveals a molecule 210 A long with three distinct functional domains arranged along a backbone of two extraordinarily long alpha-helices. A central domain at the bend of the hairpin-like structure mediates specific recognition and binding to an outer-membrane receptor. A second domain mediates translocation across the outer membrane via the TonB transport pathway; the TonB-box recognition element of colicin Ia is on one side of three 80 A-long helices arranged as a helical sheet. A third domain is made up of 10 alpha-helices which form a voltage-activated and voltage-gated ion conducting channel across the plasma membrane of the target cell. The two 160 A-long alpha-helices that link the receptor-binding domain to the other domains enable the colicin Ia molecule to span the periplasmic space and contact both the outer and plasma membranes simultaneously during function.
authors.affiliation: S-964 Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448, USA.
authors.forename: M
authors.initials: M
authors.lastname: Wiener
authors.forename: D
authors.initials: D
authors.lastname: Freymann
authors.forename: P
authors.initials: P
authors.lastname: Ghosh
authors.forename: R M
authors.initials: RM
authors.lastname: Stroud
mesh_terms: D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D003087:Colicins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D007473:Ion Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation
chemical_list: D003087:Colicins; D007473:Ion Channels