Expression and properties of feline herpesvirus type 1 gD (hemagglutinin) by a recombinant baculovirus.
We constructed a recombinant baculovirus expressing feline herpesvirus type I (FHV-1) gD in insect cells (Sf9 cells). The expressed product was identified as FHV-1 gD by a panel of monoclonal antibodies specific for the FHV-1 gD, and had an apparent molecular mass of approximately 49 kDa, which was less than that of the authentic FHV-1 gD. When the FHV-1 gD protein were expressed in Sf9 cells and CRFK cells in the presence of tunicamycin, the FHV-1 gD exhibited a molecular mass of 41 kDa. It was shown that the gD protein was transported to the surface of recombinant virus-infected Sf9 cells when examined by membrane-immunofluorescence analysis, and that the gD expressed on the surface of Sf9 cells adsorbed feline erythrocytes. Mice inoculated with a lysate of Sf9 cells expressing FHV-1 gD induced antibodies with virus-neutralizing and hemagglutination-inhibition activities. Therefore, the expressed gD appears to be biologically authentic. These data suggested that recombinant FHV-1 gD produced in Sf9 cells may be a useful immunogen as a feline vaccine.
Maeda
K
K
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Ono
M
M
Kawaguchi
Y
Y
Niikura
M
M
Okazaki
K
K
Yokoyama
N
N
Tokiyoshi
Y
Y
Tohya
Y
Y
Mikami
T
T
D000818:Animals; D002371:Cat Diseases / Q000821:virology; D002415:Cats; D004912:Erythrocytes / Q000276:immunology / Q000821:virology; D006389:Hemagglutinins, Viral / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D006561:Herpes Simplex / Q000662:veterinary / Q000821:virology; D051379:Mice; D009500:Neutralization Tests; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D018139:Simplexvirus / Q000235:genetics*; D018411:Spodoptera / Q000235:genetics; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D006389:Hemagglutinins, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C488986:glycoprotein D, Feline herpesvirus 1