Non-muscarinic- and non-adrenergic-mediated effects of lindane on phosphoinositide hydrolysis in rat brain cortex slices.
The influence of lindane upon phosphatidylinositol hydrolysis in rat brain cortex slices has been investigated using anion-exchange chromatography in order to separate the water-soluble inositol metabolites. Acetylcholine, noradrenaline, and lindane induce the accumulation of myo-[2-3H]inositol as the water-soluble inositol metabolites. However, the cholinergic muscarinic antagonist atropine inhibited the stimulatory response of carbachol, but practically unmodified the effect that lindane has on inositol phosphate production. Also, prazosin anti-alpha 1 adrenoreceptors blocked noradrenaline-induced phosphoinositide hydrolysis, but had no effect on lindane-induced increase of inositol phosphate levels. The results suggest that lindane does not exert a general effect on the receptor-stimulated formation of inositol phosphates by both muscarinic and alpha 1-adrenergic agonists.
Pajuelo
L
L
Departamento de Bioquimica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
Sánchez-Alonso
J A
JA
del Hoyo
N
N
Pulido
J A
JA
Pérez-Albarsanz
M A
MA
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D006868:Hydrolysis; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011941:Receptors, Adrenergic / Q000187:drug effects*; D011976:Receptors, Muscarinic / Q000187:drug effects*
D007306:Insecticides; D010716:Phosphatidylinositols; D011941:Receptors, Adrenergic; D011976:Receptors, Muscarinic; D001556:Hexachlorocyclohexane; D001285:Atropine; D002217:Carbachol; D009638:Norepinephrine; D011224:Prazosin