Micro-assay method for evaluating the allergenicity of the major soybean allergen, Gly m Bd 30K, with mouse antiserum and RBL-2H3 cells.
A micro-assay method for evaluating the allergenicity of soybean allergen was developed by using the mouse antiserum against Gly m Bd 30K, a major soybean allergen, and RBL-2H3, a rat mucosal mast cell line. The antiserum against Gly m Bd 30K was prepared by subcutaneously immunizing BALB/c mice with the allergen. The behavior by affinity-chromatography and the properties against heat treatment show that the reaginic activity of the antiserum resided in the IgE antibody specific for Gly m Bd 30K. The developed assay method is shown to be useful for simulating IgE mediated type-I allergy and to be highly sensitive for detecting the allergen.
Yamanishi
R
R
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
Tsuji
H
H
Bando
N
N
Yoshimoto
I
I
Ogawa
T
T
D000485:Allergens; D000818:Animals; D052179:Antigens, Plant; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005512:Food Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D006358:Hot Temperature; D007106:Immune Sera / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000276:immunology* / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D030262:Soybean Proteins; D018675:Toxicity Tests / Q000379:methods; D001619:beta-N-Acetylhexosaminidases / Q000187:drug effects / Q000378:metabolism
D000485:Allergens; D052179:Antigens, Plant; C103236:Gly m BD 30K protein, Glycine max; D007106:Immune Sera; D007074:Immunoglobulin G; D010940:Plant Proteins; D030262:Soybean Proteins; D007073:Immunoglobulin E; D001619:beta-N-Acetylhexosaminidases