Interleukin-1 beta potentiates bradykinin-induced macromolecular efflux from hamster oral mucosa.
The purpose of this study was to determine whether interleukin-1 beta (IL-1 beta) elicits macromolecular efflux from the in situ oral mucosa and whether it amplifies that evoked by bradykinin. Using intravital microscopy, we found that suffusion of recombinant human IL-1 beta (50 ng/ml) had no significant effects on leaky site formation and increase in clearance of fluorescein isothiocyanate-labeled dextran (mol mass 70 kDa) from the hamster cheek pouch. However, it significantly potentiated bradykinin-induced macromolecular efflux (P < 0.05). The potentiating effects of IL-1 beta on bradykinin-induced responses were abrogated by a bradykinin B2-receptor antagonist and by a recombinant human IL-1-receptor antagonist. They were not mediated by substance P, prostaglandins, or changes in vasomotor tone. IL-1 beta had no significant effects on adenosine-induced macromolecular efflux. Collectively, these data indicate that IL-1 beta potentiates bradykinin-induced macromolecular efflux from the in situ hamster oral mucosa in a specific fashion. We suggest that this interaction could play a role in the pathogenesis of oral mucosa inflammation.
Gao
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Department of Medicine, University of Illinois at Chicago, USA.
Rubinstein
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D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D065168:Bradykinin Receptor Antagonists; D006224:Cricetinae; D004357:Drug Synergism; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D046911:Macromolecular Substances; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects* / Q000378:metabolism*; D064729:Neurokinin-1 Receptor Antagonists; D011994:Recombinant Proteins
D065168:Bradykinin Receptor Antagonists; D007375:Interleukin-1; D046911:Macromolecular Substances; D064729:Neurokinin-1 Receptor Antagonists; D011994:Recombinant Proteins; D000241:Adenosine; D001920:Bradykinin; D007213:Indomethacin