title: Myosin binding subunit of smooth muscle myosin phosphatase at the cell-cell adhesion sites in MDCK cells.
abstract: We examined the intracellular localization of the myosin binding subunit (MBS) of smooth muscle myosin phosphatase. In MDCK cells in a confluent monolayer of polarized epithelial sheet, MBS was concentrated to the cell-cell adhesion sites. Double-immunofluorescence analysis with anti-MBS and anti-beta-catenin antibodies showed that MBS was mainly localized at the adherens junction. Furthermore, MBS was translocated reversibly between the cytosol and the cell-cell adhesion sites during the formation and disappearance of cell-cell contacts. These data suggest that MBS may play an important role in the regulation of the cell-cell adhesion.
authors.affiliation: Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Inagaki
authors.forename: M
authors.initials: M
authors.lastname: Nishizawa
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Fujioka
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: S
authors.initials: S
authors.lastname: Tsujino
authors.forename: K
authors.initials: K
authors.lastname: Matsuzawa
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Kaibuchi
authors.forename: M
authors.initials: M
authors.lastname: Inagaki
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry* / Q000502:physiology; D002448:Cell Adhesion; D002450:Cell Communication; D002460:Cell Line; D016764:Cell Polarity; D002645:Chickens; D004285:Dogs; D009130:Muscle, Smooth / Q000201:enzymology* / Q000502:physiology; D043263:Myosin-Light-Chain Phosphatase; D009218:Myosins / Q000737:chemistry* / Q000502:physiology; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000502:physiology; D051381:Rats
chemical_list: D002352:Carrier Proteins; D010749:Phosphoprotein Phosphatases; D043263:Myosin-Light-Chain Phosphatase; D009218:Myosins