Effects of an Escherichia coli ilvA mutant gene encoding feedback-resistant threonine deaminase on L-isoleucine production by Brevibacterium flavum.
A mutated ilvA gene of Escherichia coli encoding a threonine deaminase that is resistant to feedback inhibition by L-isoleucine was obtained. It was functional in Brevibacterium flavum, and a wild strain of B. flavum into which it was introduced became able to convert exogeneous L-homoserine and L-threonine to L-isoleucine. When it was introduced into a L-threonine-producing B. flavum strain, the transformant accumulated 20 g/liter L-isoleucine from 100 g/liter glucose.
Hashiguchi
K
K
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Kojima
H
H
Sato
K
K
Sano
K
K
D001951:Brevibacterium / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D007532:Isoleucine / Q000096:biosynthesis* / Q000494:pharmacology; D009154:Mutation; D013912:Threonine / Q000096:biosynthesis; D013913:Threonine Dehydratase / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D014169:Transformation, Bacterial
D007532:Isoleucine; D013912:Threonine; D013913:Threonine Dehydratase