title: Effects of temperature on hematologic and serum biochemical profiles of hybrid striped bass (Morone chrysops x Morone saxatilis).
abstract: OBJECTIVE
  To investigate the effect of water temperature on hematologic and biochemical analytes in hybrid striped bass.
  
  
  METHODS
  Hybrid striped bass (reciprocal cross: female Morone chrysops x male M saxatilis) maintained in 2,000-L tanks with undergravel filters.
  
  
  METHODS
  Fish were acclimated to 10, 18, 24, and 29 C water for 6 weeks prior to sample collection. Hematologic and serum biochemical profiles were then determined. Values were compared among the various temperatures, and with reference intervals previously determined.
  
  
  RESULTS
  Most values were within or slightly outside the established reference intervals. The following analytes deviated notably from the reference interval: leukocyte, lymphocyte, and monocyte counts were lower than the reference intervals at 10 C; glucose values were lower at 10 and 18 C; calcium values were higher at 10 and 18 C; and total protein, albumin, globulin, and chloride values were higher at 29 C.
  
  
  CONCLUSIONS
  Separate reference intervals should be developed for analytes which, because of temperature, deviate notably from the reference interval. Modifications of the established reference intervals, by including fish from varied temperatures, should allow use of one reference interval for analytes, with only slight variation attributable to temperature.
  
  
  CONCLUSIONS
  Determining the effects of temperature on the hematologic and biochemical values helps develop clinical pathology as a diagnostic tool in fish.
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA.
authors.forename: T C
authors.initials: TC
authors.lastname: Hrubec
authors.forename: J L
authors.initials: JL
authors.lastname: Robertson
authors.forename: S A
authors.initials: SA
authors.lastname: Smith
mesh_terms: D000064:Acclimatization; D000469:Alkaline Phosphatase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001492:Bass / Q000097:blood* / Q000235:genetics; D001786:Blood Glucose / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis*; D002118:Calcium / Q000097:blood*; D003433:Crosses, Genetic; D004573:Electrolytes / Q000097:blood; D004906:Erythrocyte Count; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006824:Hybridization, Genetic; D007958:Leukocyte Count; D018655:Lymphocyte Count; D008297:Male; D009000:Monocytes; D010976:Platelet Count; D012016:Reference Values; D012709:Serum Albumin / Q000032:analysis; D012712:Serum Globulins / Q000032:analysis; D013696:Temperature
chemical_list: D001786:Blood Glucose; D001798:Blood Proteins; D004573:Electrolytes; D006454:Hemoglobins; D012709:Serum Albumin; D012712:Serum Globulins; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase; D002118:Calcium