The nucleotide sequence of the translated and untranslated regions of a cDNA for myotoxin a from the venom of prairie rattlesnake (Crotalus viridis viridis).
Myotoxin a, isolated from C. viridis viridis venom, is a myonecrotizing agent present in many snake venoms. A cDNA library was prepared from mRNA obtained from the venom glands of a C. viridis viridis. The complete base sequence of a cDNA corresponding to an mRNA encoding myotoxin a was determined. The 5' untranslated region has 15 nucleotides, while the 3' untranslated region has 109 nucleotides. The translated portion of the myotoxin a cDNA encodes a start methionine, a signal peptide, the myotoxin a peptide sequence, and an additional lysine residue. It is likely that myotoxin a is secreted as the cDNA encodes a signal peptide immediately 5' to the myotoxin a peptide code.
Norris
J W
JW
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins 80523, USA.
Fry
R M
RM
Tu
A T
AT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003435:Crotalid Venoms / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D018076:DNA, Complementary / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000187:drug effects; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000737:chemistry; D014118:Toxins, Biological / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*
D003435:Crotalid Venoms; D018076:DNA, Complementary; D012333:RNA, Messenger; D014118:Toxins, Biological; C018932:myotoxin A