Inhibitory effect of green tea on injury to a cultured renal epithelial cell line, LLC-PK1.
When cells from a cultured renal epithelial cell line, LLC-PK1, were cultured under hypoxic conditions (oxygen concentration of 2% or less) before reoxygenation was applied (95% air, 5% CO2), the leakage of lactate dehydrogenase (LDH) into the medium increased. This phenomenon was inhibited in the presence of dimethyl sulfoxide, a hydroxyl radical scavenger, suggesting the involvement of free radicals. Such oxidative stress was significantly inhibited by a green tea extract, and more potently by a tannin mixture. On the other hand, under ordinary culture conditions (95%, air, 5% CO2), there was cell injury, although the LDH leakage was less than that under hypoxia/reoxygenation, and such injury was inhibited by the green tea extract and the tannin mixture.
Yokozawa
T
T
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University, Japan.
Dong
E
E
Chung
H Y
HY
Oura
H
H
Nakagawa
H
H
D000818:Animals; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D018374:LLC-PK1 Cells; D018384:Oxidative Stress / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D013552:Swine; D013634:Tannins / Q000494:pharmacology; D013662:Tea / Q000737:chemistry*
D016166:Free Radical Scavengers; D005971:Glutamates; D010936:Plant Extracts; D013634:Tannins; D013662:Tea; C026166:theanine; D007770:L-Lactate Dehydrogenase; D010100:Oxygen; D004121:Dimethyl Sulfoxide