title: Detection of different metabolites in the rabbit lens by high resolution 1H NMR spectroscopy.
abstract: OBJECTIVE
  To investigate the metabolic profile of the rabbit lens using high resolution 1H NMR spectroscopy including two-dimensional shift correlated (COSY) technique.
  
  
  METHODS
  Perchloric acid extracts of the rabbit lens were analysed with a Bruker AM-500 spectrometer and the metabolites were assigned in the spectra. Some of these were also quantified.
  
  
  RESULTS
  More than 20 metabolites were detected in the perchloric acid extract of a single lens, including amino acids, nucleotides and other related compounds. Of particular importance is the ability to detect and identify glutathione, myoinositol, scyllo-inositol and taurine.
  
  
  CONCLUSIONS
  The study demonstrated the potential of 1H NMR spectroscopy for monitoring the metabolic profile of the lens in normal and pathologic conditions.
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Norwegian Institute of Technology, University of Trondheim, Norway.
authors.forename: A
authors.initials: A
authors.lastname: Midelfart
authors.forename: A
authors.initials: A
authors.lastname: Dybdahl
authors.forename: S
authors.initials: S
authors.lastname: Gribbestad
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002682:Chinchilla; D007908:Lens, Crystalline / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009711:Nucleotides / Q000032:analysis*; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D000596:Amino Acids; D009711:Nucleotides