title: Histological, histochemical, and fine structural observations on the lymph node of the common seal (Phoca vitulina) and the grey seal (Halichoerus grypus).
abstract: The recent seal death epizootic prompted interest in their immune system, for which no current morphological data were available.
  
  
  
  Lymph nodes from adult harbor seals (Phoca vitulina) and grey seals (Halichoerus grypus) were investigated by light microscopy, electron microscopy, and lectin histochemistry.
  
  
  
  No significant differences in the lymph node morphology were found between the two species, and the overall organization of the nodes comprises of capsule, trabeculae, cortex, paracortex, and medulla. Capsule and trabeculae are composed of tightly packed collagen and elastic fibrils and are rich in fibroblasts, myofibroblasts, and smooth muscle cells. Unmyelinated nerve fibers are common. The cortex contains numerous secondary follicles with well-developed germinal centers and paracortical areas with high endothelial venules. Antigen-presenting cells and phagocytic macrophages were abundantly present. The medullary cords contain numerous plasma cells. Fibroblastic reticulum cells are common throughout the parenchyma and transverse the sinus. Marginal, radial, and medullary sinuses are lined by littoral cells. The visceral lining of the sinuses is marked by macrophages and by numerous mast cells.
  
  
  
  The morphology of seal lymph nodes does not differ significantly from that of terrestrial mammals. Earlier functional conclusions concerning seal lymph nodes are substantiated by this morphological study.
authors.affiliation: Anatomische Anstalt, University of Munich, Federal Republic of Germany.
authors.forename: U
authors.initials: U
authors.lastname: Welsch
authors.forename: S
authors.initials: S
authors.lastname: Schwertfirm
authors.forename: K
authors.initials: K
authors.lastname: Skirnisson
authors.forename: U
authors.initials: U
authors.lastname: Schumacher
mesh_terms: D000818:Animals; D006651:Histocytochemistry / Q000379:methods; D037102:Lectins; D008198:Lymph Nodes / Q000033:anatomy & histology* / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D046048:Seals, Earless / Q000033:anatomy & histology* / Q000276:immunology
chemical_list: D037102:Lectins