title: A unique DNA sequence of human enterotoxigenic Escherichia coli enterotoxin encoded by chromosomal DNA.
abstract: We detected Ent plasmids in 300 strains of human enterotoxigenic Escherichia coli, but one strain, E. coli 240-3, had neither a small nor a large plasmid and encoded the heat-labile enterotoxin (LTh(240-3)) gene on its chromosome. DNA sequences showed that LTh(240-3) differed by 12 and 14 base pairs from LT (LTh) and LT (LTp) from human H10407 and porcine EWD299 strains, respectively. In deduced precursor toxins, LTh(240-3), LTh and LTp differed from LTh, LTp and LTh(240-3) at nine, eight and eleven positions, respectively. These data suggest that although LTh(240-3) encoded in the chromosome is antigenically similar to LTh, it cannot be grouped with LTh due to differences in its DNA and amino acids sequences.
authors.affiliation: Department of Microbiology, Fujita Health University School of Medicine, Aichi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Imamura
authors.forename: N
authors.initials: N
authors.lastname: Kido
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Kawase
authors.forename: A
authors.initials: A
authors.lastname: Miyama
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000595:Amino Acid Sequence; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins