title: Characterization of oligodeoxyribonucleotide-polyethylene glycol conjugates by electrospray mass spectrometry.
abstract: Electrospray mass spectrometry (ESMS) was used to characterize a number of differently functionalized polyethylene glycol-oligodeoxyribonucleotide conjugates. Sample preparation was found to be crucial to obtaining quality data. The resolution and precision of ESMS allowed for the identification of individual conjugates differing in MW by a single ethylene glycol unit with an accuracy of < or = 1 (0.02% of the molecular weight). In addition, ESMS was shown to be valuable in identifying chromatographically unresolvable components of a derivatized polyethylene glycol-oligonucleotide conjugate mixture.
authors.affiliation: NeXstar Pharmaceuticals Inc., Boulder, Colorado 80301, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Tarasow
authors.forename: D
authors.initials: D
authors.lastname: Tinnermeier
authors.forename: C
authors.initials: C
authors.lastname: Zyzniewski
mesh_terms: D000577:Amides / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D009838:Oligodeoxyribonucleotides; D063088:Phosphoramides; D010756:Phosphoric Acids / Q000378:metabolism; D011092:Polyethylene Glycols
chemical_list: D000577:Amides; D009838:Oligodeoxyribonucleotides; D063088:Phosphoramides; D010756:Phosphoric Acids; C005438:phosphoramide; D011092:Polyethylene Glycols