title: Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adeno-associated virus vectors.
abstract: We previously found that gene transduction by adeno-associated virus (AAV) vectors in cell culture can be stimulated over 100-fold by treatment of the target cells with agents that affect DNA metabolism, such as irradiation or topoisomerase inhibitors. Here we show that previous gamma-irradiation increased the transduction rate in mouse liver by up to 900-fold, and the topoisomerase inhibitor etoposide increased transduction by about 20-fold. Similar rates of hepatic transduction were obtained by direct injection of the liver or by systemic delivery via tail vein injection. Hepatocytes were much more efficiently transduced than other cells after systemic delivery, and up to 3% of all hepatocytes could be transduced after one vector injection. The presence of wild-type AAV, which contaminates many AAV vector preparations, was required to observe a full response to gamma-irradiation. Injection of mice with AAV vectors encoding human clotting factor IX after gamma-irradiation resulted in synthesis of low levels of human clotting factor IX for the 5-month period of observation. These studies show the potential of targeted gene transduction of the liver by AAV vectors for treatment of various hematological or metabolic diseases.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Koeberl
authors.forename: I E
authors.initials: IE
authors.lastname: Alexander
authors.forename: C L
authors.initials: CL
authors.lastname: Halbert
authors.forename: D W
authors.initials: DW
authors.lastname: Russell
authors.forename: A D
authors.initials: AD
authors.lastname: Miller
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000235:genetics*; D004791:Enzyme Inhibitors; D005047:Etoposide / Q000494:pharmacology; D005164:Factor IX / Q000096:biosynthesis* / Q000235:genetics; D005720:Gamma Rays; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D007275:Injections, Intravenous; D008099:Liver / Q000378:metabolism* / Q000821:virology; D051379:Mice; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D059004:Topoisomerase I Inhibitors; D014407:Tumor Cells, Cultured
chemical_list: D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D059004:Topoisomerase I Inhibitors; D005047:Etoposide; D005164:Factor IX