Identification of mRNA-rich keratinocytes in the basal/suprabasal layers of psoriatic skin.
In this study, we examined the relative amounts of mRNA expressed in normal versus psoriatic epidermis, using in situ hybridization with a biotinylated oligonucleotide poly d(T) probe. The hybridization image was analyzed by Laser Scanning Confocal Microscopy (LSCM). In normal human skin, hybridization signals were detected homogeneously throughout the epidermis, mostly in nucleus. These signals disappeared following RNase T2 or RNase A treatment, indicating that the target for this probe is RNA; by implication, mRNA. In psoriatic lesions, the overall signal intensity was significantly elevated, especially in the basal/suprabasal layers. Moreover, those signals were most prominent in the cytoplasm, not in the nucleus. In contrast, the signals of uninvolved epidermis adjacent to the psoriatic lesions were indistinguishable from those of normal skin in both signal intensity and hybridization profile. Our data are consistent with the notion that one of the characteristic features of psoriasis is an elevated (or uncontrolled) synthesis of mRNA and proteins.
Yokota
K
K
Department of Dermatology, Hokkaido University School of Medicine, Sapporo, Japan.
Matsue
H
H
Shibaki
A
A
Kawashima
T
T
Kobayashi
H
H
Ohkawara
A
A
D001707:Biopsy, Needle; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000648:ultrastructure*; D006801:Humans; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D011565:Psoriasis / Q000276:immunology / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012016:Reference Values
D012333:RNA, Messenger