Cloning, functional expression and tissue distribution of rabbit alpha 1d-adrenoceptor.
We have cloned a cDNA encoding rabbit alpha 1d-adrenoceptor from the rabbit liver cDNA library. The deduced amino-acid sequence of this clone encodes a protein of 576 amino acids that shows strong sequence homology to previously cloned human, rat and mouse alpha 1d-adrenoceptors. The pharmacological radioligand binding properties of this clone expressed in COS-7 cells were similar to those of rat alpha 1d-adrenoceptors. Competitive RT/PCR assays revealed wide tissue distribution of the alpha 1d-adrenoceptor mRNA in rabbit, especially abundant in vas deferens, aorta, prostate and cerebral cortex.
Suzuki
F
F
Department of Pharmacology, Fukui Medical School, Japan.
Miyamoto
S
S
Takita
M
M
Oshita
M
M
Watanabe
Y
Y
Kakizuka
A
A
Narumiya
S
S
Taniguchi
T
T
Muramatsu
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D018340:Receptors, Adrenergic, alpha-1 / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
C547719:ADRA1D protein, human; C547720:Adra1d protein, mouse; C547721:Adra1d protein, rat; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1