Use of Chemically Modified Silica with beta-Diketoamine Groups for Separation of alpha-Lactoalbumin from Bovine Milk Whey by Affinity Chromatography
Silica gel surface was chemically modified with beta-diketoamine groups by reacting the silanol from the silica surface with 3-aminopropyl-triethoxysilane and 3-bromopentanedione. With this material, copper ions were adsorbed from aqueous solutions. The chemical analysis of the silica-gel-immobilized acetylacetone provided a quantity of 0.67 mmol g-1 of organic groups attached to the support and 0.63 mmol g-1 of copper. This material was used as a stationary phase in IMAC (immobilized metal affinity chromatography), to separate alpha-lactoalbumin from bovine milk whey. The results showed an efficient separation in the chromatographic column. The possibility of reutilization of the stationary phase was also investigated.
Gambero
A
Departamento de Quimica, F.C. Unesp, Bauru, S.P., 17033-360, Brazil
Kubota
LT
Gushikem
Y
Airoldi
C
Granjeiro
JM
Taga
EM
Alcantara
EFC