title: Two-phase endolymphatic hydrops: a new dynamic guinea pig model.
abstract: The classical guinea pig model for Meniere's disease, in which endolymphatic hydrops was achieved by destruction of the endolymphatic sac and obliteration of the endolymphatic duct, is a non-physiological profound model with shortcomings in relation to Meniere's disease as seen in patients. We developed a more subtle animal model; the two-phase endolymphatic hydrops. This model is based on a combination of chronic endolymphatic sac dysfunction, induced by slight destruction of the most distal part of the endolymphatic sac, and acute stress-induced endolymph production by stimulation of the Na/K-ATPase in the stria vascularis with aldosterone. Light microscopy of the fluid compartments of four groups of cochleas was used to examine them for the presence of endolymphatic hydrops: i) Normal (control) cochleas showed no hydrops; ii) some of the non-operated (no destruction) aldosterone-treated cochleas showed small degrees of hydrops mainly present in the basal turns; iii) mild dissection of the endolymphatic sac without administration of aldosterone produced a hydrops which was mainly present in the cochlear apex; iv) combination of chronic endolymphatic sac dysfunction and acute attacks of endolymph production by aldosterone administration revealed the most severe degrees of hydrops in all cochlear windings, damage to cochlear structures, and cellular disturbances of the epithelial lining of the endolymphatic sac. This new model may represent a more physiologic and dynamic approach to Meniere's disease and may explain the etiology of many symptoms in patients such as the fluctuant nature and the types of sensoneuronal hearing losses.
authors.affiliation: Department of Otorhinolaryngology, University Hospital Groningen, The Netherlands.
authors.forename: E A
authors.initials: EA
authors.lastname: Dunnebier
authors.forename: J M
authors.initials: JM
authors.lastname: Segenhout
authors.forename: H P
authors.initials: HP
authors.lastname: Wit
authors.forename: F W
authors.initials: FW
authors.lastname: Albers
mesh_terms: D000450:Aldosterone / Q000493:pharmacokinetics / Q000494:pharmacology; D000818:Animals; D003051:Cochlea / Q000187:drug effects / Q000503:physiopathology; D004195:Disease Models, Animal; D018159:Endolymphatic Hydrops / Q000473:pathology* / Q000503:physiopathology; D004712:Endolymphatic Sac / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D006168:Guinea Pigs; D008575:Meniere Disease / Q000473:pathology / Q000503:physiopathology; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism
chemical_list: D000450:Aldosterone; D000254:Sodium-Potassium-Exchanging ATPase