Oxidative stress-induced increase in intracellular Ca2+ and Ca(2+)-induced increase in oxidative stress: an experimental model using dissociated rat brain neurons.
In order to study the oxidative stress-induced change in intracellular concentration of Ca2+ ([Ca2+]i) and Ca(2+)-induced oxidative stress, effects of hydrogen peroxide and ionomycin, a calcium ionophore, on rat cerebellar neurons were examined using a flow cytometer and fluorescent dyes: fluo-3 for monitoring [Ca2+]i; 2',7'-dichlorofluorescin, for reactive oxygen species; and 5-chloromethylfluorescein, for cellular nonprotein thiols. Oxidative stress induced by hydrogen peroxide dose-dependently increased [Ca2+]i and decreased the content of nonprotein thiols. Ionomycin increased oxidative metabolism and decreased the content of nonprotein thiols. Results suggest that oxidative stress induces an increase in [Ca2+]i while an increase in [Ca2+]i increases oxidative stress in neurons.
Oyama
Y
Y
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Okazaki
E
E
Chikahisa
L
L
Nagano
T
T
Sadakata
C
C
D000814:Aniline Compounds; D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology*; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004996:Ethidium; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014966:Xanthenes
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D014966:Xanthenes; C059715:Fluo-3; D004996:Ethidium; D002118:Calcium