Bacillus subtilis bacteriophage SPP1 terminase has a dual activity: it is required for the packaging initiation and represses its own synthesis.
The B. subtilis bacteriophage SPP1 terminase, encoded by genes 1 and 2, is required for the initiation of headful packaging. The DNA segment to which gene 1 product (G/P) binds includes the pacL and pacR sites and the late PL1 and PL2 promoters from which genes 1 to 7 are transcribed. When SPP1wt or SPP1sus115 (gene 6-) phages were used to infect a B. subtilis sup0 strain, the gene 1 to 7 mRNA synthesis was reduced at late times of infection. This was not observed, however, when either chloramphenicol was added 7 min after infection with SPP1wt or when SPP1sus114 (gene 1-) or SPP1sus19 (gene 2-) were used to infect B. subtilis sup0 cells. These results suggest that the terminase enzyme functions as a repressor of its own transcription. G/P and B. subtilis RNA polymerase (RP) bind to the pacL segment, which contains the PL1 and PL2 promoter region. The binding of G/P to the pacL site does not seem to exclude RP from the promoters, despite of the overlapping of their binding sites. It is likely that the terminase protein does not repress transcription by a mere steric hindrance of RP binding.
Chai
S
S
Centro Nacional de Biotecnología, CSIC, Madrid, Spain.
Szepan
U
U
Alonso
J C
JC
D017103:Bacillus Phages / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D001412:Bacillus subtilis / Q000821:virology; D004247:DNA / Q000096:biosynthesis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D009876:Operon; D014176:Protein Biosynthesis; D012808:Sigma Factor / Q000378:metabolism; D014158:Transcription, Genetic; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics; D019065:Virus Assembly / Q000502:physiology*
D012808:Sigma Factor; D054334:Viral Regulatory and Accessory Proteins; C073671:SPP1 pac region protein, Bacteriophage SPP1; D004247:DNA; C063505:RNA polymerase sigma A; D012321:DNA-Directed RNA Polymerases; D004706:Endodeoxyribonucleases; C030894:terminase