Rapid gene transfer into cultured hippocampal neurons and acute hippocampal slices using adenoviral vectors.
Primary cultures of hippocampal neurons were infected with an adenovirus coding for beta-galactosidase. Expression could be detected as early as 4 h after infection and steadily increased to high levels at 24 h without evidence for a functional impairment of the infected neurons. Similarly, adenovirus-mediated gene transfer into acute hippocampal slices was detectable 4 h after infection and could be localized to discrete areas of the CA1 region by microinjection of the virus stock solution. Infected slices were still suitable for electrophysiological experiments.
Griesbeck
O
O
Abteilung Neurochemie, Max-Planck-Institut für Psychiatrie, Martinsried, Germany. griesbec@alf.biochem.mpg.de
Korte
M
M
Gravel
C
C
Bonhoeffer
T
T
Thoenen
H
H
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D006624:Hippocampus / Q000648:ultrastructure / Q000821:virology*; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase