Selenite suppression of cadmium-induced testicular apoptosis.
The characteristic apoptotic ladder-like patterns of rat testicular DNA on agarose gel electrophoresis which results from treatment with CdCl2 are suppressed by the administration of Na2SeO3. The examination of testicular tissue using an ELISA programmed cell death detection procedure confirmed this selenite suppression of cadmium-induced apoptosis. The administration of the Na2SeO3 at either 0.5, 1, 2 h prior to or 0.5, 1, 2 h after the administration of the CdCl2 appear to be almost equally effective at suppressing the apoptotic response. These results are in accord with previous studies on the Na2SeO3 suppression of cadmium induced necrotic changes in tissues and suggest that Na2SeO3 interferes with both necrosis and apoptosis.
Jones
M M
MM
Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.
Xu
C
C
Ladd
P A
PA
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002104:Cadmium / Q000633:toxicity*; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D004347:Drug Interactions; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar; D018038:Sodium Selenite / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*
D002104:Cadmium; D004247:DNA; D018038:Sodium Selenite