[The detection of tramadol and its metabolites in urine by chromatographic methods].
The authors recommend detecting a compound drug tramadol and its metabolites in urinary extracts by thin-layer, gas-chromatographic, high-pressure liquid, and other chromatographic methods. The metabolites were identified with due consideration for their fragmentation during an electron strike thereof and their trimethylsilyl derivatives, mobility during separation in a thin layer, and results of selective extraction at various pH. The proposed scheme of biotransformation includes the formation of products of O- and N- demethylation, N, N- and N,O-didemethylation and hydroxylation of the cyclohexane fragment. The Rf metabolite values and their mass spectra are presented. During gas chromatographic analysis at an evaporator temperature at least 250 degrees C tramadol undergoes thermal destruction with the formation of water molecules and cyclohexane structure.
Veselovskaia
N V
NV
Kislun
Iu V
IuV
Eremin
S K
SK
Izotov
B N
BN
Viatchanina
A P
AP
Dorogokupets
O B
OB
Morozova
E B
EB
Kovalenko
A E
AE
D000701:Analgesics, Opioid / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods / Q000706:statistics & numerical data; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000706:statistics & numerical data; D002855:Chromatography, Thin Layer / Q000379:methods / Q000706:statistics & numerical data; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D014147:Tramadol / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000652:urine*
D000701:Analgesics, Opioid; D014147:Tramadol