Absence of linkage for "endothelial" nitric oxide synthase locus to blood pressure in Dahl rats.
Nitric oxide is thought to be involved in blood pressure regulation. Nitric oxide synthase (NOS) genes are logical candidates for genetic hypertension. Of the three known forms of NOS, the "neuronal" and "inducible" Nos genes have been tested as candidate genes for causing inherited hypertension in Dahl salt-sensitive rats. In the present work, we analyzed the endothelial Nos gene, designated Nos3, directly and indirectly for cosegregation with blood pressure in six F2 populations independently generated from crosses of Dahl salt-sensitive rats with rats of various other strains. The Nos3 alleles did not cosegregate with blood pressure in these populations. Therefore, Nos3 is an improbable, if not impossible, candidate gene for causing hypertension in the Dahl salt-sensitive rat.
Deng
A Y
AY
Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo 43699-0008, USA. adeng@vortex.mco.edu
Rapp
J P
JP
D000818:Animals; D017920:Antiporters / Q000235:genetics; D001483:Base Sequence; D001794:Blood Pressure / Q000235:genetics*; D027963:Chloride-Bicarbonate Antiporters; D017931:DNA Primers / Q000737:chemistry; D004195:Disease Models, Animal; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D006973:Hypertension / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D011919:Rats, Inbred Strains
D017920:Antiporters; D027963:Chloride-Bicarbonate Antiporters; D017931:DNA Primers; D005819:Genetic Markers; D019001:Nitric Oxide Synthase