title: Chromosomal deletions in Streptomyces griseus that remove the afsA locus.
abstract: We have recently constructed a physical map of the Streptomyces griseus 2247 genome using the restriction enzymes AseI and DraI, which revealed that this strain carries a 7.8 Mb linear chromosome. Based on this map, precise macrorestriction fragment and cosmid maps were constructed for both ends of the chromosome, which localized the afsA gene 150 Kb from the left end. Two afsA- mutants were found to have suffered chromosomal deletions that removed the afsA locus. The sizes of the deletions were 20 and 130 Kb at the right end and 180 and 350 kb at the left end, respectively. Hybridization experiments using cosmids carrying a deletion endpoint indicated that the ends of the chromosome in the mutants were fused to form a circular chromosome.
authors.affiliation: Department of Fermentation Technology, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Lezhava
authors.forename: D
authors.initials: D
authors.lastname: Kameoka
authors.forename: H
authors.initials: H
authors.lastname: Sugino
authors.forename: K
authors.initials: K
authors.lastname: Goshi
authors.forename: H
authors.initials: H
authors.lastname: Shinkawa
authors.forename: O
authors.initials: O
authors.lastname: Nimi
authors.forename: S
authors.initials: S
authors.lastname: Horinouchi
authors.forename: T
authors.initials: T
authors.lastname: Beppu
authors.forename: H
authors.initials: H
authors.lastname: Kinashi
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000096:biosynthesis; D002876:Chromosomes, Bacterial / Q000235:genetics* / Q000648:ultrastructure; D003360:Cosmids; D017353:Gene Deletion; D005798:Genes, Bacterial; D015183:Restriction Mapping; D013305:Streptomyces griseus / Q000235:genetics* / Q000378:metabolism
chemical_list: C025035:A-factor (Streptomyces); D015107:4-Butyrolactone