Protective effect of vitamin E on chromium (VI)-induced cytotoxicity and lipid peroxidation in primary cultures of rat hepatocytes.
Pretreatment of primary cultures of rat hepatocytes with alpha-tocopherol succinate (vitamin E) for 20 h prior to exposure to K2Cr2O7 resulted in a marked decrease of chromium (VI)-induced cytotoxicity, as evaluated by the leakage of lactate dehydrogenase, without affecting cellular uptake and the subcellular distribution of chromium. The levels of chromium (VI)-induced lipid peroxidation, as monitored by malondialdehyde formation, were also inhibited by pretreatment with the vitamin. Pretreatment with vitamin E normalized the levels of nonenzymatic antioxidants such as glutathione and vitamin C suppressed by dichromate, and caused a distinct accumulation of vitamin E in hepatocytes. However, vitamin E pretreatment did not affect the activities of enzymatic antioxidants including glutathione reductase, superoxide dismutase, and catalase suppressed by dichromate. These results indicate that the protective effect of vitamin E against chromium (VI)-induced cytotoxicity as well as lipid peroxidation, may be associated more with the level of nonenzymatic antioxidants than the activity of enzymatic antioxidants.
Susa
N
N
Department of Veterinary Public Health, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Ueno
S
S
Furukawa
Y
Y
Sugiyama
M
M
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D002840:Chromates / Q000378:metabolism / Q000633:toxicity*; D004336:Drug Antagonism; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D017680:Potassium Compounds / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D018675:Toxicity Tests; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D002840:Chromates; D017680:Potassium Compounds; D014810:Vitamin E; C027373:potassium chromate(VI)