title: Effects of interferon-alpha (IFN-alpha) administration on leucocytes in healthy humans.
abstract: Plasma concentrations of IFN-alpha are increased in several inflammatory conditions. Several lines of evidence indicate that IFN-alpha has anti-inflammatory properties. To study the effects of IFN-alpha on leucocyte subsets and activation and on cytokines, we administered IFN-alpha (rhIFN-alpha2b; 5 x 10(6) U/m2) to eight healthy human subjects in a randomized controlled cross-over study and analysed changes in circulating leucocytes and parameters for neutrophil and monocyte activation. After administration of IFN-alpha, neutrophil counts increased, monocyte counts decreased transiently, whereas the number of lymphocytes, basophils and eosinophils showed a sustained decrease. IFN-alpha administration was also associated with neutrophil activation, reflected in an increase in the plasma concentrations of elastase-alpha1-antitrypsin complexes and lactoferrin. Serum neopterin, a marker for monocyte activation, was significantly increased 10 h after administration of IFN-alpha. IFN-alpha significantly increased plasma concentrations of IL-6, IL-8 and IL-10. Although IL-1 and tumour necrosis factor (TNF) remained undetectable, plasma concentrations of soluble TNF receptors p55 and p75 increased after IFN-alpha administration. We conclude that IFN-alpha induces multiple alterations in the distribution and functional properties of leucocytes. IFN-alpha exerts pro- as well as anti-inflammatory effects within the cytokine network.
authors.affiliation: Department of Internal Medicine, Academic Medical Centre, University of Amsterdam, The Netherlands.
authors.forename: E P
authors.initials: EP
authors.lastname: Corssmit
authors.forename: R
authors.initials: R
authors.lastname: Heijligenberg
authors.forename: C E
authors.initials: CE
authors.lastname: Hack
authors.forename: E
authors.initials: E
authors.lastname: Endert
authors.forename: H P
authors.initials: HP
authors.lastname: Sauerwein
authors.forename: J A
authors.initials: JA
authors.lastname: Romijn
mesh_terms: D000328:Adult; D018592:Cross-Over Studies; D016207:Cytokines / Q000032:analysis; D006801:Humans; D007370:Interferon Type I / Q000008:administration & dosage*; D007958:Leukocyte Count / Q000187:drug effects; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D008297:Male; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D011994:Recombinant Proteins
chemical_list: D016207:Cytokines; D007370:Interferon Type I; D011994:Recombinant Proteins