title: Inhibition of self-splicing group I intron RNA: high-throughput screening assays.
abstract: High-throughput screening assays have been developed to rapidly identify small molecule inhibitors targeting catalytic group I introns. Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis carinii or from bacteriophage T4 have been investigated. In vitro biochemical assays amenable to high-throughput screening have been established. Small molecules that inhibit the functions of group I introns have been identified. These inhibitors should be useful in better understanding ribozyme catalysis or in therapeutic intervention of group I intron-containing microorganisms.
authors.affiliation: Department of Chemistry, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, MI 48106, USA. meih@aa.wl.com
authors.forename: H Y
authors.initials: HY
authors.lastname: Mei
authors.forename: M
authors.initials: M
authors.lastname: Cui
authors.forename: S T
authors.initials: ST
authors.lastname: Sutton
authors.forename: H N
authors.initials: HN
authors.lastname: Truong
authors.forename: F Z
authors.initials: FZ
authors.lastname: Chung
authors.forename: A W
authors.initials: AW
authors.lastname: Czarnik
mesh_terms: D017122:Bacteriophage T4 / Q000235:genetics; D002384:Catalysis; D007438:Introns; D011010:Pneumocystis / Q000235:genetics; D012313:RNA / Q000235:genetics*; D012326:RNA Splicing
chemical_list: D012313:RNA