Improvement of blood and fly gut processing for PCR diagnosis of trypanosomosis.
We have adapted a simple and efficient technique to detect trypanosomes in human blood, without DNA purification, and increased the sensitivity threshold to 1 parasite in 1 ml. We have then applied it for detection of parasites in midguts of tsetse flies, negative by microscopy. This technique has been developed for field conditions and could greatly facilitate epidemiological studies.
Penchenier
L
L
Laboratoire de recherche sur les trypanosomes OCEAC, BP 288, Yaoundé, Cameroun.
Dumas
V
V
Grebaut
P
P
Reifenberg
J M
JM
Cuny
G
G
D000818:Animals; D006801:Humans; D007422:Intestines / Q000469:parasitology; D008722:Methods; D016133:Polymerase Chain Reaction; D014347:Trypanosoma brucei gambiense; D014348:Trypanosoma congolense; D014353:Trypanosomiasis, African / Q000097:blood / Q000175:diagnosis*; D014370:Tsetse Flies / Q000469:parasitology*