[Axonal regeneration of retinal ganglion cells].
We have developed retinal culture system of adult mammals to investigate neural regeneration from adult retinal ganglion cells (RGC). In this culture system, neurites were regenerated from RGCs of adult retinal explants. Investigation of neurotrophic effects on the neural regeneration showed that some interleukins and neurotrophins enhanced neurite regeneration from adult rat RGCs. We also found that the adult human retina had the ability of neural regeneration and that neurotrophins enhanced this ability. A novel neurotrophic factor secreted by adult rat hepatocytes also enhanced neurite regeneration not only in adult mice but also in aged RGCs. This result indicated the novel hepatocyte secreted factor is an activator which enhances neural regeneration of the aged retina. We concluded that even adult aged RGCs had the ability of axonal regeneration after injury and that neurotrophic factors might enhanced these abilities. Therefore neurotrophic factors might have practicable applications in drug treatments for intractable disease of the neural retina and optic nerve. Future progress of neuroscience is expected to rescue the retina from various diseases, and to render possible the transplantation of the retina and optic nerve.
Takano
M
M
Department of Ophthalmology, Yokohama City University School of Medicine, Kanagawa-ken, Japan.
D000328:Adult; D000818:Animals; D001369:Axons / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012165:Retinal Ganglion Cells / Q000502:physiology*
D007375:Interleukin-1; D015850:Interleukin-6; D009414:Nerve Growth Factors