A role for the sodium, potassium adenosine triphosphatase (Na+,K+ ATPase) enzyme in degranulation of rat basophilic leukaemia cells.
BACKGROUND
A circulating inhibitor of the sodium, potassium adenosine triphosphatase (Na+,K+ ATPase) enzyme has been described in allergic subjects. Recent studies have suggested that the Na+,K+ ATPase, enzyme may be involved in the signal transduction pathways of various cell types and that inhibition of its activity can modulate histamine release from basophils and mast cells.


OBJECTIVE
The purpose of this study was to determine if modulation of Na+,K+ ATPase activity alters degranulation in the 2H3 subline of rat basophilic leukaemia cells (RBL-2H3), a mucosal mast cell model bearing high-affinity Fc receptors for IgE.


METHODS
Degranulation was measured by the release of both exogenous serotonin and endogenous histamine. Na+,K+ ATPase activity was assessed by ouabain-sensitive [86rubidium] uptake ([86Rb] uptake) and ex situ enzyme activity.


RESULTS
Ouabain-sensitive [86Rb] uptake and degranulation increased in parallel and in a dose-response fashion with increasing Fc receptor cross-linking. Additionally, incubation with ouabain, a known inhibitor of Na+,K+ ATPase activity, decreased both anti-IgE and calcium ionophore-induced degranulation, but increased spontaneous degranulation, each in a dose-response manner. Moreover, the effect of ouabain on degranulation was reversed by rinsing and mimicked by other known inhibitors of Na+,K+ ATPase activity. Finally, in the absence of anti-IgE or calcium ionophore, stimulation of ouabain-sensitive [86Rb] uptake by the sodium (Na+) ionophore monensin was associated with a corresponding dose-response increase in ouabain-sensitive degranulation. These experiments demonstrate that ouabain-sensitive [86Rb] uptake increases following IgE receptor cross-linking in RBL-2H3, and that factors which modulate Na+,K+ ATPase activity in these cells may also regulate degranulation.


CONCLUSIONS
The results of this study suggest an important role for Na+,K+ ATPase activation in the signal transduction pathway of stimulated RBL-2H3.
Gentile
D A
DA
Department of Pediatrics, School of Medicine, University of Pittsburgh, Children's Hospital of Pittsburgh, PA 15213, USA.
Skoner
D P
DP
D000818:Animals; D001491:Basophils / Q000502:physiology*; D015550:Cell Degranulation / Q000502:physiology*; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006632:Histamine / Q000378:metabolism; D006636:Histamine Release; D015471:Leukemia, Basophilic, Acute / Q000201:enzymology*; D008407:Mast Cells / Q000502:physiology; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D012414:Rubidium Radioisotopes / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000502:physiology*; D014407:Tumor Cells, Cultured
D004791:Enzyme Inhibitors; D012414:Rubidium Radioisotopes; D012701:Serotonin; D010042:Ouabain; D006632:Histamine; D000254:Sodium-Potassium-Exchanging ATPase