title: Murine tissue inhibitor of metalloproteinases-4 (Timp-4): cDNA isolation and expression in adult mouse tissues.
abstract: We have isolated cDNA clones corresponding to a new member of the murine tissue inhibitor of metalloproteinase (TIMP) family, designated Timp-4. The nucleotide sequence predicts a protein of 22,609 Da that contains the characteristic 12 cysteine TIMP signature. TIMP-4 is more closely related to TIMP-2 and TIMP-3 than to TIMP-1 (48%, 45% and 38% identity, respectively). Analysis of Timp-4 mRNA expression in adult mouse tissues indicated a 1.2 kb transcript in brain, heart, ovary and skeletal muscle. This pattern of expression distinguishes Timp-4 from other Timps, suggesting that the TIMP-4 protein may be an important tissue-specific regulator of extracellular matrix remodelling.
authors.affiliation: Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
authors.forename: K J
authors.initials: KJ
authors.lastname: Leco
authors.forename: S S
authors.initials: SS
authors.lastname: Apte
authors.forename: G T
authors.initials: GT
authors.lastname: Taniguchi
authors.forename: S P
authors.initials: SP
authors.lastname: Hawkes
authors.forename: R
authors.initials: R
authors.lastname: Khokha
authors.forename: G A
authors.initials: GA
authors.lastname: Schultz
authors.forename: D R
authors.initials: DR
authors.lastname: Edwards
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D008297:Male; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D019714:Tissue Inhibitor of Metalloproteinases; D000097792:Tissue Inhibitor of Metalloproteinase-4
chemical_list: D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D008666:Metalloendopeptidases; D011506:Proteins; D012313:RNA; D019714:Tissue Inhibitor of Metalloproteinases; D000097792:Tissue Inhibitor of Metalloproteinase-4