title: High-performance chromatographic assay for the sulphoxide metabolite of 2'-deoxy-3'-thiacytidine in human urine.
abstract: A high-performance liquid chromatographic method is described for the determination in human urine of GI138870X, the sulphoxide metabolite of a novel dideoxynucleoside analogue, 2'-deoxy-3'-thiacytidine (lamivudine). GI138870X was extracted from human urine using Empore SDB RPS solid-phase extraction disks prior to reversed-phase chromatography with UV detection. The method has shown to be valid over the concentration range 0.5-100 micrograms/ml using a 0.5-ml sample volume.
authors.affiliation: Bioanalysis and Drug Metabolism Department, GlaxoWellcome Research and Develpment, Greenford, Middleses, UK.
authors.forename: R S
authors.initials: RS
authors.lastname: Plumb
authors.forename: R D
authors.initials: RD
authors.lastname: Gray
authors.forename: A J
authors.initials: AJ
authors.lastname: Harker
authors.forename: S
authors.initials: S
authors.lastname: Taylor
mesh_terms: D019380:Anti-HIV Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003499:Cyclic S-Oxides / Q000652:urine*; D006801:Humans; D019259:Lamivudine / Q000031:analogs & derivatives* / Q000652:urine
chemical_list: D019380:Anti-HIV Agents; D003499:Cyclic S-Oxides; D019259:Lamivudine; C104840:lamivudine sulfoxide