title: Autocrine IL-2-dependent growth of a newly established CD3+, CD16-, CD56+, CD57+, J(H)-, TCRbeta-, TCRgamma- leukemia cell line (NOI-90).
abstract: Leukemic cells from a 45-year-old male patient with a CD3+, CD56+, CD57+, CD7+ acute lymphoblastic leukemia were cultured in vitro in the absence of any added growth factor for up to 6 years and a continuous lymphoblastoid cell line (NOI-90) was established. NOI-90 cells have the same phenotype and karyotype as initial leukemic cells. Southern blot of DNA from NOI-90 cells showed that TCRbeta, TCRgamma, and J(H) were in germ line. Two and 25% of NOI-90 cells were positive when stained with the IOT14 and 7G7/B6 moAbs, which recognize the CD25 molecule (IL-2R alpha chain); moreover, 4% and 13% of the cells were positive when stained with the TU-27 and mik beta3 moAbs which recognize the CD122 molecule (IL-2Rbeta chain). Equilibrium binding experiments with radiolabelled IL-2 revealed the presence of a small number of high affinity IL-2R on both fresh and continuously growing cells. Media conditioned by NOI-90 cells could induce proliferation of an IL-2-dependent cell line and this IL-2 activity could be detected by a sensitive immunoenzymatic assay using antibodies recognizing distinct epitopes of IL-2. Moreover, IL-2 activity could be adsorbed by immunoaffinity on anti-IL-2 polyclonal purified IgG and the retained molecule displayed a m.w. of 14.5 kDa in SDS-PAGE. In addition, IL-2 immunoreactive molecules could be revealed in the cytoplasm of the cells. Finally, IL-2 fixed on the cell membrane could be detected by indirect immunofluorescence. Although added IL-2 could not induce cell proliferation, monoclonal antibodies against CD25, CD122 and IL-2 could specifically inhibit spontaneous cell proliferation in a dose-dependent manner. NOI-90 cells failed to demonstrate any cytotoxic activity against the K-562, Raji or Daudi cells. These findings indicate that NOI-90 cells are of non-T, non-B, origin lacking NK activity but proliferate under an autocrine pathway which involves, at least partly, the IL-2/IL-2R system.
authors.affiliation: Oncogenese Appliquee, INSERM U268, Hopital Paul Brousse, Villejuif, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Sahraoui
authors.forename: M
authors.initials: M
authors.lastname: Perraki
authors.forename: M
authors.initials: M
authors.lastname: Theodoropoulou
authors.forename: M
authors.initials: M
authors.lastname: Allouche
authors.forename: A
authors.initials: A
authors.lastname: Tsapis
authors.forename: A
authors.initials: A
authors.lastname: Ammar
authors.forename: C
authors.initials: C
authors.lastname: Clemenceau
authors.forename: S
authors.initials: S
authors.lastname: Bokogiorgou
authors.forename: T
authors.initials: T
authors.lastname: Yacci
authors.forename: G
authors.initials: G
authors.lastname: Katrinakis
authors.forename: C
authors.initials: C
authors.lastname: Jasmin
authors.forename: V
authors.initials: V
authors.lastname: Georgoulias
mesh_terms: D017252:CD3 Complex / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis; D018959:CD57 Antigens / Q000032:analysis; D017077:Culture Media, Conditioned / Q000494:pharmacology; D054278:Embryonal Carcinoma Stem Cells; D017809:Fatal Outcome; D019084:Fluorescent Antibody Technique, Indirect; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D017452:Receptors, IgG / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D018959:CD57 Antigens; D017077:Culture Media, Conditioned; D007143:Immunoglobulin Heavy Chains; D007376:Interleukin-2; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D017452:Receptors, IgG