title: TNF-alpha, IL-1 alpha, IL-6 and ICAM-1 expression in human keratinocytes stimulated in vitro with Escherichia coli heat-shock proteins.
abstract: Bacterial heat-shock proteins (HSPs) from Escherichia coli (GroES, GroEL and DnaK) were studied for their ability to induce by themselves the expression and release of tumour necrosis factor-alpha (TNF-alpha), interleukin-1 alpha (IL-1 alpha), interleukin-6 (IL-6) and intercellular adhesion molecule-1 (ICAM-1) by cultured human keratinocytes. The surface expression of ICAM-1 was also investigated. In the supernatants of untreated cells none or a minimal amount of these molecules was found. After 48 h of stimulation with GroEL significant amounts of TNF-alpha, IL-1 alpha, IL-6 and soluble ICAM-1 were detected, reaching maximum concentrations at 1 microgram ml-1. The same effect was elicited by DnaK but to a lesser extent. Treatment of keratinocytes with GroEL and DnaK also increased TNF-alpha, IL-1 alpha, IL-6 and ICAM-1 mRNA levels. GroES showed significant activity only on the expression and release of IL-6. GroEL and DnaK were also able to up-regulate the surface expression of ICAM-1 on keratinocytes. The effects on ICAM-1 expression seemed to be directly due to HSPs and not mediated via cytokines. Furthermore, these effects were due to the properties of HSPs because they were inhibited by specific monoclonal antibodies. These findings support the potential role of HSPs in modulating cell interactions during immunological and inflammatory responses in the skin.
authors.affiliation: Istituto di Microbiologia, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Marcatili
authors.affiliation: Istituto di Microbiologia, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
authors.forename: Gabriella Cipollaro
authors.initials: GC
authors.lastname: de I'Ero
authors.affiliation: Istituto di Microbiologia, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
authors.forename: Marilena
authors.initials: M
authors.lastname: Galdiero
authors.affiliation: Istituto di Microbiologia, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Folgore
authors.affiliation: Istituto di Clinica Dermosifilopatica, Seconda Universit degli Studi di Napoli, Facolt di Medicina e Chirurgia, Larghetto S.Aniello a Caponapoli, 2, 80138 Napoli, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Petrillo
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D018835:Chaperonin 10 / Q000494:pharmacology; D018834:Chaperonin 60 / Q000494:pharmacology; D004926:Escherichia coli / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000209:etiology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1