title: Meiosis reinitiation of mussel oocytes involves L-type voltage-gated calcium channel.
abstract: In the present work we assessed the involvement of L-type voltage opening Ca2+ channels in KCl-induced meiosis reinitiation of metaphase-arrested blue mussel (Mytilus galloprovincialis) oocytes by performing binding assays with a tritiated dihydropyridine analog (+)PN 200110. Our data reveal the existence of a single class of dihydropyridine receptors in plasma membrane-enriched rough microsome preparations of mussel oocytes. The apparent affinity (Kd) of characterized receptors equals 1.32 +/- 0.21 microM while their maximal binding capacity (Bmax) is 620 +/- 150 pmol/mg protein. The comparison of the rank order of potency of analogs tested to: 1) inhibit [(+)-[3H]PN 200110 specific binding and 2) block KCl-induced meiosis reinitiation pointed to the pharmacological profile similar to but not identical with those previously described for mammalian dihydropyridine receptors. The efficiencies of all antagonists tested are linearly related (r = 0.995) in binding-(inhibition of [(+)-[3H]PN 200110 specific binding) and biological (inhibition of meiosis reinitiation) assays thus arguing for functional involvement of L-type Ca2+ channels in oocyte activation. Reversibility of antagonist actions on meiosis reinitiation and dependence of receptor binding characteristics on a membrane polarization state further suggested such a role.
authors.affiliation: Laboratoire de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique et Ecole Normale Supérieure de Lyon, France.
authors.forename: M
authors.initials: M
authors.lastname: Tomkowiak
authors.forename: P
authors.initials: P
authors.lastname: Guerrier
authors.forename: S
authors.initials: S
authors.lastname: Krantic
mesh_terms: D000818:Animals; D001665:Binding Sites; D049872:Bivalvia / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D020746:Calcium Channels, L-Type; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004095:Dihydropyridines / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D017275:Isradipine / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D014316:Tritium
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D004095:Dihydropyridines; D014316:Tritium; D011189:Potassium Chloride; C038806:1,4-dihydropyridine; D017275:Isradipine