[Purification of EcoO44I restriction endonuclease in Escherichia coli O44 isolated from an affected human].
A restriction endonuclases (ENase) designated EcoO44I was purified without non specific nucleases from enteropathogenic Escherichia coli O44 Hiromi strain of affected human origin. The yield was 1, 100 units/g of wet cells. The EcoO44I ENase recognized and cleaved the specific sequence of 5'-GGTCTC-3' (1/5) as was the case with Eco31I or BsaI ENase. Because of the stability and high yield, EcoO44I would be useful for recombinant DNA technology after isolation of EcoO44-positive, avirulent mutant strains of E. coli O44 Hiromi.
Miyahara
M
M
Shinohara
N
N
Mise
K
K
D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans
D004262:DNA Restriction Enzymes