Antitumor effect of CPT-11, a camptothecin derivative, on human testicular tumor xenografts in nude mice.
OBJECTIVE
The antitumor effect of CPT-11, a camptothecin derivative, on two human testicular embryonal carcinomas (TTSC-2 and TTSC-3) heterotransplanted into-nude mice was studied.


METHODS
Tumor-bearing nude mice were given daily intraperitoneal injections of the anticancer drugs in 0.1 ml saline 3 times at 3-day intervals. At the end of the experiments tumors were resected and subjected to light-microscopic observation.


RESULTS
When 10, 30 and 50 mg/kg of CPT-11 was administered to tumor-bearing mice intraperitoneally, the antitumor effect of CPT-11 was observed dose-dependently in both TTSC-2 and TTSC-3. When 30 mg/kg of CPT-11 was administered in combination with CDDP, complete tumor regression was observed in both TTSC-2 and TTSC-3 tumors. Histological findings correlated well with the decrease in tumor volume of treated tumors. No mice died after treatment with CPT-11 in a single-agent and combination chemotherapy.


CONCLUSIONS
Chemotherapy with CPT-11 was an effective and safe method against human testicular tumors heterotransplanted in nude mice.
Miki
T
T
Department of Urology, Osaka University Medical School, Japan.
Sawada
M
M
Nonomura
N
N
Kojima
Y
Y
Okuyama
A
A
Maeda
O
O
Saiki
S
S
Kotake
T
T
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000627:therapeutic use*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D016190:Carboplatin / Q000008:administration & dosage / Q000031:analogs & derivatives; D002945:Cisplatin / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D005047:Etoposide / Q000008:administration & dosage; D006801:Humans; D000077146:Irinotecan; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009944:Organoplatinum Compounds / Q000008:administration & dosage; D018243:Teratocarcinoma / Q000188:drug therapy*; D013736:Testicular Neoplasms / Q000188:drug therapy*; D059004:Topoisomerase I Inhibitors; D014183:Transplantation, Heterologous; D014747:Vinblastine / Q000008:administration & dosage
D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D009944:Organoplatinum Compounds; D059004:Topoisomerase I Inhibitors; C058816:DWA 2114; D014747:Vinblastine; D005047:Etoposide; D000077146:Irinotecan; C053989:nedaplatin; D016190:Carboplatin; D002945:Cisplatin; D002166:Camptothecin