Guide RNA-independent and guide RNA-dependent uridine insertion into cytochrome b mRNA in a mitochondrial lysate from Leishmania tarentolae. Role of RNA secondary structure.
A primer extension assay was used for the detection of uridine insertions occurring in vitro in synthetic pre-edited cytochrome b mRNA during incubation with a Leishmania tarentolae mitochondrial extract. Two different activities were detected that inserted uridines within the first two editing sites: one that is dependent on the secondary structure of the mRNA but is independent of both exogenous and endogenous guide RNA, and a second that does not put the same structural constraints on the mRNA, but is dependent on the presence of a cognate guide RNA.
Connell
G J
GJ
Howard Hughes Medical Institute, University of California, Los Angeles, California 90095-1662, USA.
Byrne
E M
EM
Simpson
L
L
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003573:Cytochrome b Group / Q000235:genetics*; D007891:Leishmania / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA; D017394:RNA, Guide, Kinetoplastida / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014529:Uridine / Q000235:genetics*
D003573:Cytochrome b Group; C034477:RNA primers; D012333:RNA, Messenger; D012313:RNA; D014529:Uridine; D017394:RNA, Guide, Kinetoplastida