Cloning and sequence analysis of a Bothrops jararaca cDNA encoding a precursor of seven bradykinin-potentiating peptides and a C-type natriuretic peptide.
A 1.8-kb cDNA clone was isolated from a Bothrops jararaca venom gland cDNA library that encodes a 256-aa precursor for bradykinin-potentiating peptides (angiotensin-converting enzyme inhibitors) and a C-type natriuretic peptide (CNP). The seven bradykinin-potentiating peptides are aligned tandemly after the hydrophobic signal peptide sequence, followed by a putative intervening sequence and a CNP at the C terminus. Northern blot analysis indicated the predominant expression of a 1.8-kb mRNA in the venom glands as well as in the spleen and the brain. Two lower intensity mRNA bands of 3.5 kb and 5.7 kb also hybridized to the cDNA clone. Radioimmunoassay for the CNP was performed using the antiserum against rat CNP. The presence of CNP immunoreactivity was detected in the low molecular weight fraction of the Bothrops jararaca venom.
Murayama
N
N
School of Pharmaceutical Sciences, Showa University, Shinagawa, Tokyo, Japan.
Hayashi
M A
MA
Ohi
H
H
Ferreira
L A
LA
Hermann
V V
VV
Saito
H
H
Fujita
Y
Y
Higuchi
S
S
Fernandes
B L
BL
Yamane
T
T
de Camargo
A C
AC
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D001483:Base Sequence; D017837:Bothrops / Q000235:genetics*; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000235:genetics* / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type; D011498:Protein Precursors / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification; D011863:Radioimmunoassay; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D000806:Angiotensin-Converting Enzyme Inhibitors; D003435:Crotalid Venoms; D018076:DNA, Complementary; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011506:Proteins; D020098:Natriuretic Peptide, C-Type