The aetiological significance of human papillomavirus in bladder cancer.
OBJECTIVE
To determine the prevalence of human papillomavirus (HPV) DNA types 6, 11 and 16 in histological sections of human bladder cancer.


METHODS
Fifty-five formalin-fixed, paraffin-embedded bladder tumour specimens were analysed for the presence of HPV infection using Southern blotting DNA hybridization and radiolabelled probes for HPV DNA types 6/11 and 16.


RESULTS
Despite the detection of HPV DNA type 6, 11 and 16 in positive control samples and the successful detection of HPV DNA in anogenital cancer using the same technique, no HPV DNA was found in any of the bladder tumour specimens examined.


CONCLUSIONS
Using a technique with proven efficacy in the detection of HPV DNA from histological specimens, no HPV DNA was present in any of the bladder tumours examined. This finding is in agreement with most recently published studies suggesting that HPV has no significant role in the development of human bladder cancer.
Boucher
N R
NR
Department of Urology, Royal Hallamshire Hospital, Sheffield, UK.
Scholefield
J H
JH
Anderson
J B
JB
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D002295:Carcinoma, Transitional Cell / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000150:complications*; D016719:Radioimmunodetection; D014412:Tumor Virus Infections / Q000150:complications*; D001749:Urinary Bladder Neoplasms / Q000821:virology*
D004279:DNA, Viral