title: Nitrosated glycine derivatives as a potential source of O6-methylguanine in DNA.
abstract: The nitrosated bile acid conjugate N-nitrosoglycocholic acid reacts with DNA to give, rise to several adducts including O6-carboxymethylguanine and, unexpectedly, O6-methylguanine (O6-MG). O6-MG is well established as a toxic and promutagenic lesion and is a substrate for the DNA repair protein O6-alkylguanine-DNA-alkyltransferase. In contrast, O6-carboxymethylguanine is not repaired by this protein. Similar results have been obtained for other nitrosated glycine derivatives, which suggests that O6-MG, which has been observed in DNA from human gastrointestinal tissues, may be derived from intragastric nitrosation of glycine or related compounds.
authors.affiliation: MRC Toxicology Unit, Hodgkin Building, University of Leicester, United Kingdom.
authors.forename: D E
authors.initials: DE
authors.lastname: Shuker
authors.forename: G P
authors.initials: GP
authors.lastname: Margison
mesh_terms: D004247:DNA / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism*; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D009602:Nitrosamines / Q000378:metabolism*
chemical_list: D009602:Nitrosamines; D006147:Guanine; C034937:N-nitrosoglycocholic acid; D004247:DNA; C008449:O-(6)-methylguanine; D005998:Glycine