Inhibition of metastasis by a dialysable factor in fetal bovine serum in B16 melanoma cells.
Fetal bovine serum (FBS) supplemented to the culture medium inhibited the metastasis of B16BL6 melanoma cells in a dose-dependent manner. This metastasis-inhibiting activity was accompanied by growth-promoting activity, up to the concentration of 10% FBS. However, among the clones isolated from B16BL6 cells, there were clones in which FBS significantly inhibited the metastasis without promoting their growth. Dialysis (cutoff M.W. 8000 Da) removed the metastasis-inhibiting activity but not the growth-promoting activity from FBS. These results indicate that FBS has metastasis-inhibiting activity which is independent of growth-promoting activity, and that the metastasis-inhibiting activity is carried by molecules removed by dialysis.
Ota
T
T
Department of Pathology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
Maeda
M
M
Matsui
T
T
Kohno
H
H
Tanino
M
M
Odashima
S
S
D000818:Animals; D002417:Cattle; D003470:Culture Media / Q000737:chemistry; D016207:Cytokines / Q000302:isolation & purification / Q000494:pharmacology*; D003956:Dialysis; D005312:Fetal Blood / Q000737:chemistry*; D008297:Male; D008546:Melanoma, Experimental / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL
D003470:Culture Media; D016207:Cytokines