Tamm-Horsfall glycoprotein (THG) is a binder for surface membrane proteins on blood cells and glomerular mesangial cells.
A macromolecule with a molecular weight of 90-100 kDa was purified from normal human pregnancy urine. The molecule was proved to be the Tamm-Horsfall glycoprotein (THG) by Western blot analysis. The macromolecule contains carbohydrate as detected by an enzyme immunoassay. Functionally, the glycoprotein can adhere to and stimulate the thymidine incorporation of human mononuclear cells (MNC) in modest degree via its membranotropic property. In addition to MNC, the protein can also bind to the surface of human polymorphonuclear neutrophils (PMN), red blood cells (RBC) and rat glomerular mesangial cells (RMC). Western blot analysis of various cell lysates with/without proteinase K pretreatment before cell lysis revealed that a 60 kDa and a molecule larger that 94 kDa on the surface of PMN, a 60 kDa protein on MNC and a 32 kDa protein on RBC are the binding molecules for THG. In contrast, many proteins on the surface of RMC could be bound by THG. Immunoprecipitation of membranous iodinated MNC lysates also confirmed that the 60 kDa molecule on MNC is the binding protein for THG. A number of monosaccharide including N-acetylneuraminic acid, N-acetyl-galactosamine, N-acetyl-glucosamine and alpha-methyl-D-mannoside could not inhibit the mitogenic effect of THG on human mononuclear cells. These results suggest that THG is capable of reacting with surface membrane proteins on different cells, but not through the specific carbohydrate-containing lectin-like receptors on the cell surface.
Yu
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CL
Section of Allergy, Immunology & Rheumatology, Veterans General Hospital-Taipei, National Yang-Ming University, Taiwan.
Tsai
C Y
CY
Sun
K H
KH
Hsieh
S C
SC
Tsai
Y Y
YY
Tsai
S T
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Yu
H S
HS
Han
S H
SH
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism* / Q000652:urine; D004232:Diuretics / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005920:Glomerular Mesangium / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000097:blood* / Q000378:metabolism* / Q000652:urine; D009005:Monosaccharides / Q000494:pharmacology; D009088:Mucoproteins / Q000097:blood* / Q000378:metabolism* / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood* / Q000378:metabolism* / Q000652:urine; D051381:Rats; D012680:Sensitivity and Specificity; D058949:Uromodulin
D001798:Blood Proteins; D004232:Diuretics; D008565:Membrane Proteins; D009005:Monosaccharides; D009088:Mucoproteins; D011257:Pregnancy Proteins; C548612:UMOD protein, human; C548614:Umod protein, rat; D058949:Uromodulin