Decrease in the expression of a novel TGF beta1-inducible and ras-recision gene, TSC-36, in human cancer cells.
TSC-36, a TGF beta1-inducible gene, encodes a polypeptide that shows significant similarity to SPARC (secreted protein rich in cysteine), and follistatin, an activin-binding protein. The expression of the TSC-36 gene was reported to be extinguished in v-ras-transformed mouse fibroblastic cells, and also was found also to be abrogated in cells transformed with v-myc. The level of expression was, however, not affected in cells transformed with v-src, v-abl, or v-raf. The TSC-36 cDNA was first isolated from mouse cells, and recently its rat and human homologues have been reported which show striking similarity with each other. In various human tumor cells, TSC-36 mRNA was almost undetectable. TSC-36 mRNA was detected in various mouse organs, but its level was the highest in the lung. TSC-36 mRNA level was the highest in the lung among mouse organs, and on in situ hybridization, the TSC-36 transcript was detected in the alveolar epithelium but not in the bronchial epithelium. These features suggest possible usage of TSC-36 as one of the markers in human tumors.
Mashimo
J
J
Department of Microbiology, Showa University School of Pharmaceutical Sciences, Shinagawa-ku, Tokyo, Japan.
Maniwa
R
R
Sugino
H
H
Nose
K
K
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D038702:Follistatin-Related Proteins; D005786:Gene Expression Regulation; D011905:Genes, ras; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009856:Oncogene Proteins, Viral / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution
D038702:Follistatin-Related Proteins; D006023:Glycoproteins; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger