title: Analysis of bovine herpesvirus 4 genomic regions located outside the conserved gammaherpesvirus gene blocks.
abstract: Bovine herpesvirus 4 (BHV-4) DNA sequences located outside the gene blocks conserved among the gammaherpesviruses BHV-4, herpesvirus saimiri (HVS) and Epstein-Barr virus (EBV) were analysed. Twelve potential open reading frames (ORFs) were found. Protein database comparisons showed that no ORF translation products were similar to proteins encoded by alpha- or betaherpesviruses. Nevertheless, six of the ORFs were homologous in amino acid sequences to proteins encoded by HVS but apparently not to those encoded by EBV. Furthermore, the location and orientation of these six ORFs in the BHV-4 genome were similar to the corresponding ORFs in the HVS genome. No genes homologous to known cellular genes were found in the BHV-4 genome; this feature is the major difference between the BHV-4 and HVS genomes with regards to the overall gene content.
authors.affiliation: Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liege, Belgium. viro@stat.fmv.ulg.ac.be
authors.forename: P
authors.initials: P
authors.lastname: Lomonte
authors.forename: M
authors.initials: M
authors.lastname: Bublot
authors.forename: V
authors.initials: V
authors.lastname: van Santen
authors.forename: G M
authors.initials: GM
authors.lastname: Keil
authors.forename: P P
authors.initials: PP
authors.lastname: Pastoret
authors.forename: E
authors.initials: E
authors.lastname: Thiry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017124:Conserved Sequence; D018142:Gammaherpesvirinae / Q000235:genetics*; D016679:Genome, Viral; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA