title: Use of capillary electrophoresis to measure dimerization of glycopeptide antibiotics.
abstract: Capillary electrophoretic methods were used to examine dimerization and estimate dimerization constants (Kdim) for the glycopeptide antibiotics vancomycin, ristocetin A, and LY264826 (A82846B). The Kdim for LY264826 was 60- and 200-fold higher than the Kdim for ristocetin A and vancomycin, respectively. Dimerization of vancomycin measured in the presence of the cell wall analog N, N'-diacetyl-L-Lys-D-Ala-D-Ala was enhanced 200-fold; however, dimerization of ristocetin A was antagonized by the presence of N, N'-diacetyl-L-Lys-D-Ala-D-Ala. The relative differences in Kdim determined by capillary electrophoresis in general follow the same trend as those observed using nuclear magnetic resonance spectroscopy and sedimentation equilibrium.
authors.affiliation: Infectious Diseases Research, Eli Lilly and Company, Indianapolis, Indiana, 46285, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: LeTourneau
authors.forename: N E
authors.initials: NE
authors.lastname: Allen
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007700:Kinetics; D011487:Protein Conformation; D012310:Ristocetin / Q000737:chemistry; D014640:Vancomycin / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; C057893:chloroorienticin A; D012310:Ristocetin; C016719:ristocetin A; D014640:Vancomycin