title: Heterogeneity of expression of the calcium-binding protein calretinin in human colonic cancer cell lines.
abstract: We searched for the presence of calretinin (CR) in 12 colonic cancer cell lines using immunohistochemistry, Western blot analysis and the reverse transcriptase-polymerase chain reaction (RT-PCR). We were able to demonstrate that calretinin is expressed in the cell lines HT-29, WiDr, LoVo, LS180, CO112, CO115. SW480, SW620, COLO205 and SK-CO-1, while no detectable amounts were found in the cell lines SW1116 and Caco-2. In general, rapidly proliferating cell lines expressed calretinin, whereas the protein was absent from cell lines with a low multiplication rate. A possible role for calretinin in maintaining the proliferative cycle of tumor cells is discussed.
authors.affiliation: Institute of Histology and General Embryology, University of Fribourg, Switzerland.
authors.forename: V
authors.initials: V
authors.lastname: Gotzos
authors.forename: B
authors.initials: B
authors.lastname: Schwaller
authors.forename: J C
authors.initials: JC
authors.lastname: Gander
authors.forename: M
authors.initials: M
authors.lastname: Bustos-Castillo
authors.forename: M R
authors.initials: MR
authors.lastname: Celio
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D015153:Blotting, Western; D018938:Caco-2 Cells / Q000378:metabolism; D064032:Calbindin 2; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C576764:CALB2 protein, human; D064032:Calbindin 2; D009363:Neoplasm Proteins; D064030:S100 Calcium Binding Protein G