title: pH regulation of voltage-dependent K+ channels in canine pulmonary arterial smooth muscle cells.
abstract: Voltage-dependent K+ currents (Kv) may play a role in hypoxic pulmonary vaso constriction. The effects of changes in extracellular pH (pHo) and intracellular pH (pHi) on Kv currents in smooth muscle cells isolated from canine pulmonary artery were studied using the amphotericin B perforated-patch technique for whole-cell recording. Under these conditions, cellular mechanisms for pHi regulation remain intact, and the effects of pHo were examined by directly changing the pH of external solutions and changes in pHi were produced by external application of weak extracellular acids and bases and the cation/H+ ionophore, nigericin. Ca(2+)-free external solutions were used to isolate whole-cell Kv currents from contaminating Ca(2+)-activated K+ currents. Extracellular acidification (pHo = 6.4-7.0) reduced Kv currents, produced a positive voltage shift in steady-state activation and reduced maximum Kv conductance (gK). Extracellular alkalinization (pHo = 8.0-8.4) increased Kv currents, produced a small negative voltage shift in steady-state activation, and increased gK. Intracellular acidification produced by exposure of cells to external sodium butyrate (20 mM) or nigericin (5 micrograms/ml) increased Kv currents, produced a negative voltage shift in steady-state activation, and increased gK. Intracellular alkalinization produced by exposure of cells to external trimethylamine (20 mM) reduced Kv currents, produced a small positive voltage shift in steady-state activation and reduced gK. These results suggest that the effects of pHo and pHi on Kv currents are distinctly different, but are consistent with reported effects of pHo and pHi on hypoxic pulmonary vasoconstriction, suggesting that such modulation may be mediated in party by pH-induced alterations in Kv channel activity.
authors.affiliation: Department of Physiology and Cell Biology/351, University of Nevada School of Medicine, Reno 89557-0046, USA.
authors.forename: D S
authors.initials: DS
authors.lastname: Ahn
authors.forename: J R
authors.initials: JR
authors.lastname: Hume
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism; D004285:Dogs; D004594:Electrophysiology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011651:Pulmonary Artery / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D018118:Chloride Channels; D015221:Potassium Channels; D000666:Amphotericin B; D002118:Calcium