Docetaxel enhances the expression of E-cadherin and carcinoembryonic antigen (CEA) on human colon cancer cell lines in vitro.
The in vitro effects of docetaxel on the human colon carcinoma cell lines-HT-29 and SW620 were studied by measuring the expression of the differentiation markers E-cadherin and CEA with a recently developed immunohistochemical assay. Docetaxel was shown to increase the expression of E-cadherin and CEA in the low concentration range of 3 x 10(-10) M - 3 x 10(-9) M for HT-29 cells and 0.6 - 3 x 10(-9) M for SW620 cells. Docetaxel strongly increased CEA (270%) and E-cadherin (160%) expression in HT-29 cells, compared with both marker expression in SW620 cells (60%). E-cadherin and CEA expression were found to be inversely correlated to the antiproliferating potential of docetaxel, which inhibits the cell proliferation of HT-29 cells at an IC50 of 6 x 10(-10) M and of SW620 cells at 8 x 10(-10) M. The data suggest, that in addition to its antiproliferative activity docetaxel also exhibits a strong differentiation inducing capacity at concentrations < 10(-9) M in vitro. These docetaxel effects were more pronounced on the differentiated HT-29 cells than on the poorly differentiated SW620 cells.
Eckert
K
K
Institut fur Pharmazie, Freie Universität Berlin, Germany.
Fuhrmann-Selter
T
T
Maurer
H R
HR
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D015820:Cadherins / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000737:chemistry / Q000188:drug therapy* / Q000473:pathology; D000077143:Docetaxel; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000494:pharmacology; D043823:Taxoids; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D015820:Cadherins; D002272:Carcinoembryonic Antigen; D043823:Taxoids; D000077143:Docetaxel; D017239:Paclitaxel