title: Photodynamic therapy of rat endometrium sensitized with tin ethyl etiopurpurin.
abstract: OBJECTIVE
  To evaluate endometrial ablation in the rat using photodynamic therapy and the photosensitizer tin ethyl etiopurpurin (SnET2).
  
  
  METHODS
  Laboratory research.
  
  
  METHODS
  A pharmaceutical and device manufacturing company.
  
  
  METHODS
  Forty-five healthy female rats (age 8-10 wks).
  
  
  METHODS
  Groups of three to five rats were given SnET2 by either intrauterine or intravenous administration. Light treatment was given at either 3 or 24 hours after SnET2 administration at a light dose of 75, 150, or 200 J/cm.
  
  
  RESULTS
  A fluorescence detection system was employed to determine relative drug uptake of SnET2 into uterine tissue. The highest levels of SnET2 were detected at 3 hours. After light treatment, responses of uterine tissues were evaluated histologically. The best endometrial ablation was seen when SnET2 was given by intrauterine administration with light treatment at 150 J/cm 24 hours later. A consistent transmural response was seen with this route of administration at 200 J/cm. Intravenous SnET2 gave inconsistent responses. In light-only controls, all light doses caused no tissue response. The depth of necrosis in tissues treated with photodynamic therapy were light-dose dependent.
  
  
  CONCLUSIONS
  With either route of SnET2 administration, drug uptake was confirmed and a light-dose-dependent response in the walls of rat uterine horns was demonstrated.
authors.affiliation: PDT Inc., 7408 Hollister Avenue, Santa Barbara, CA 93117, USA.
authors.forename: G B
authors.initials: GB
authors.lastname: Rocklin
authors.forename: H G
authors.initials: HG
authors.lastname: Kelly
authors.forename: S C
authors.initials: SC
authors.lastname: Anderson
authors.forename: L E
authors.initials: LE
authors.lastname: Edwards
authors.forename: R J
authors.initials: RJ
authors.lastname: Gimpelson
authors.forename: R E
authors.initials: RE
authors.lastname: Perez
mesh_terms: D000818:Animals; D004717:Endometrium / Q000528:radiation effects*; D005260:Female; D010778:Photochemotherapy; D011166:Porphyrins / Q000008:administration & dosage; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000473:pathology / Q000528:radiation effects
chemical_list: D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C063166:tin etiopurpurin