title: Recognition of cytoplasmic yeast antigens of Cryptococcus neoformans var. neoformans and Cryptococcus neoformans var. gattii by immune human sera.
abstract: The humoral immune response of patients infected with Cryptococcus neoformans var. neoformans and C. neoformans var. gattii to cytoplasmic (non-capsular) antigens from the two varieties of Cryptococcus has been investigated. Cytoplasmic antigens from C. neoformans (one clinical isolate and one acapsular mutant of var. neoformans and two clinical isolates from var. gattii) were subject to isoelectric focusing, SDS-PAGE and Western blotting; patients sera was then used in the immunoenzyme development of the Western blots. The humoral response from the 20 patients (all HIV+) infected with var. neoformans against the var. neoformans antigens was predominantly IgG based, with a large number of bands recognised: the most commonly recognised bands were at 26, 52, 74, 100, 115 and 144 kDa. The IgM response was less pronounced and the IgA response was practically non-existent. The humoral response of the sera from the 15 patients (all but one HIV-) infected with var. gattii against var. gattii antigens was also predominantly IgG based with bands at 37, 55, 65, 74, 94 and 115 kDa being most commonly recognised. Periodate treatment of cytoplasmic antigens reduced the intensity of antigen recognition, though it did not absolutely destroy reactivity to any individual antigen. Comparison of immunodevelopment of cytoplasmic antigens from both varieties grown at 25 degrees C and 37 degrees C revealed that culture temperature made no differences in the number of bands recognised although there were differences in the intensity of recognition. This is the first report on the pattern of serological recognition of the non-capsular antigens from the two varieties of Cryptococcus and it identifies a number of major antigenic components.
authors.affiliation: Dermatology Unit, St Johns Institute of Dermatology, Guy's Hospital, London, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Hamilton
authors.forename: J I
authors.initials: JI
authors.lastname: Figueroa
authors.forename: L
authors.initials: L
authors.lastname: Jeavons
authors.forename: R A
authors.initials: RA
authors.lastname: Seaton
mesh_terms: D000908:Antibodies, Fungal / Q000097:blood*; D000946:Antigens, Fungal / Q000276:immunology*; D015153:Blotting, Western; D003453:Cryptococcosis / Q000175:diagnosis / Q000382:microbiology; D003455:Cryptococcus neoformans / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology* / Q000382:microbiology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007525:Isoelectric Focusing; D008297:Male; D012703:Serotyping
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal