Photodynamic therapy of rat endometrium sensitized with tin ethyl etiopurpurin.
OBJECTIVE
To evaluate endometrial ablation in the rat using photodynamic therapy and the photosensitizer tin ethyl etiopurpurin (SnET2).


METHODS
Laboratory research.


METHODS
A pharmaceutical and device manufacturing company.


METHODS
Forty-five healthy female rats (age 8-10 wks).


METHODS
Groups of three to five rats were given SnET2 by either intrauterine or intravenous administration. Light treatment was given at either 3 or 24 hours after SnET2 administration at a light dose of 75, 150, or 200 J/cm.


RESULTS
A fluorescence detection system was employed to determine relative drug uptake of SnET2 into uterine tissue. The highest levels of SnET2 were detected at 3 hours. After light treatment, responses of uterine tissues were evaluated histologically. The best endometrial ablation was seen when SnET2 was given by intrauterine administration with light treatment at 150 J/cm 24 hours later. A consistent transmural response was seen with this route of administration at 200 J/cm. Intravenous SnET2 gave inconsistent responses. In light-only controls, all light doses caused no tissue response. The depth of necrosis in tissues treated with photodynamic therapy were light-dose dependent.


CONCLUSIONS
With either route of SnET2 administration, drug uptake was confirmed and a light-dose-dependent response in the walls of rat uterine horns was demonstrated.
Rocklin
G B
GB
PDT Inc., 7408 Hollister Avenue, Santa Barbara, CA 93117, USA.
Kelly
H G
HG
Anderson
S C
SC
Edwards
L E
LE
Gimpelson
R J
RJ
Perez
R E
RE
D000818:Animals; D004717:Endometrium / Q000528:radiation effects*; D005260:Female; D010778:Photochemotherapy; D011166:Porphyrins / Q000008:administration & dosage; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000473:pathology / Q000528:radiation effects
D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C063166:tin etiopurpurin