In vitro effects of anthelmintics on the histochemistry of Haemonchus contortus and Trichuris globulosa.
In vitro incubation of Haemonchus contortus (RUD., 1803) and Trichuris globulosa (v. LINSTOW, 1901) were proformed for 10-12 h in Tyrode's solution and 10 and 50 micrograms/ml concentrations each of albendazole (ABZ), fenbendazole (FBZ), thiophenate (TP), dl-tetramisole and oxyclozanide (TO), dl-tetramisole HCI (TMS) and levamisole HCI (LMS) to study morphological and histochemical alterations. The major structural changes observed after treatment with all the drugs were vacuolation in the intestine of H. contortus except with TP treatment and disruption of the epithelium in T. globulosa except with TMS treatment. The other major alteration in T. globulosa was the loss of muscle striations after TP, TO, TMS and LMS treatments. All the six anthelmintics reduced the quantity of neutral mucopolysaccharides in the intestine of H. contortus and T. globulosa except with TMS and LMS treatments in the latter. Acidic mucopolysaccharides detected in the microvilli of the intestine of H. contortus were lost after TO and ABZ treatments. The loss of lipids from the intestine was evident after TO, ABZ, FBZ and TP treatments in H. contortus and by all the drugs in T. globulosa. LMS treatment caused accumulation of very large lipid droplets in the intestine of H. contortus.
Kaur
M
M
Department of Zoology, Punjab Agricultural University, Ludhiana, India.
Sood
M L
ML
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D050260:Carbohydrate Metabolism; D006041:Goats; D006189:Haemonchus / Q000187:drug effects*; D050356:Lipid Metabolism; D012756:Sheep; D014258:Trichuris / Q000187:drug effects*
D000871:Anthelmintics