title: SUG1, a putative transcriptional mediator and subunit of the PA700 proteasome regulatory complex, is a DNA helicase.
abstract: Yeast SUG1 was originally characterized as a transcriptional mediator for the GAL4 transactivator. A similar role in vertebrates was suggested by the ligand-enhanced interaction between mammalian homologues of yeast SUG1 and the ligand-dependent activating domain (AF-2) of nuclear receptors. SUG1 was also shown to be a component of the PA700 regulatory complex of the 26 S proteasome and a member of a large family of putative ATPases. However, no catalytic function has yet been attributed to SUG1. We show here that SUG1 is a 3'-5' DNA helicase whose activity is dependent on an intact ATP binding domain. The sedimentation heterogeneity of mammalian SUG1 suggests that it may be associated with distinct protein complexes and therefore play multiple roles.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM/ULP, Collège de France, BP163 F-67404 Illkirch Cedex, C. U. de Strasbourg, France.
authors.forename: R A
authors.initials: RA
authors.lastname: Fraser
authors.forename: M
authors.initials: M
authors.lastname: Rossignol
authors.forename: D J
authors.initials: DJ
authors.lastname: Heard
authors.forename: J M
authors.initials: JM
authors.lastname: Egly
authors.forename: P
authors.initials: P
authors.lastname: Chambon
mesh_terms: D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D004265:DNA Helicases / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D046988:Proteasome Endopeptidase Complex; D011506:Proteins / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D017421:Sequence Analysis
chemical_list: D005656:Fungal Proteins; C085834:PA700 proteasome activator; D011506:Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases; C069670:RPT6 protein, S cerevisiae; D004265:DNA Helicases