Structure determination of the O-antigenic polysaccharide from the enterotoxigenic Escherichia coli (ETEC) O101.
The O-antigenic polysaccharide of the lipopolysaccharide from the enterotoxigenic Escherichia coli O101 has been investigated. The composition and sequence of the repeating units was established by sugar and methylation analysis together with 1H and 13C NMR spectroscopy. The sequence was corroborated using the computer program CASPER. The structure of the repeating unit of the polysaccharide from E. coli O101 is as follows: -->6)-alpha-D-GlcpNAc-1-->4-alpha-D-GalpNAc-(1-->.
Staaf
M
M
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Urbina
F
F
Weintraub
A
A
Widmalm
G
G
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D012703:Serotyping
D004768:Enterotoxins; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial