An assessment of the Sartorius MD8 microbiological air sampler.
Tests described in this paper show that gelatine membrane filters used in the MD8 microbiological air sampling system collected monodispersed aerosols between 0.7 and 1.0 microns containing viable Bacillus subtilis var. niger spores, with an efficiency of 99.9995%. Gelatine membrane filters linked to the MD8 control pump system were as effective as the well established Casella slit-to-agar device for collecting some viable bacteria, nebulized under controlled experimental conditions and naturally occurring airborne micro-organisms in a pharmaceutical plant. By using a long flexible hose connection to the control pump, the head could be positioned where sampling was required in locations remote from the pump exhaust, making it suitable for microbiological monitoring in critical locations such as laminar flow stations and isolators.
Parks
S R
SR
Biosafety Investigation Unit, Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK.
Bennett
A M
AM
Speight
S E
SE
Benbough
J E
JE
D000336:Aerosols; D000391:Air Microbiology; D001412:Bacillus subtilis / Q000302:isolation & purification; D004784:Environmental Monitoring / Q000295:instrumentation; D004926:Escherichia coli / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005374:Filtration / Q000295:instrumentation; D007779:Lactobacillus acidophilus / Q000302:isolation & purification; D008567:Membranes, Artificial; D013171:Spores, Bacterial
D000336:Aerosols; D008567:Membranes, Artificial