Genetic defect of the sodium pump-defective mutant Nap-1 from the marine Vibrio alginolyticus.
The marine bacterium, Vibrio alginolyticus, has a respiratory chain-linked Na(+)-translocating NADH-quinone reductase (NQR). Among several mutant cells defective in Na+ pump activity, Nap1 was a very stable mutant and a spontaneous revertant could not be isolated from Nap1. Using genetic information from the recently sequenced nqr operon, the genetic defects in Nap1 were examined, and the sodium pump-defective mutant Nap1 was found to be caused by the insertion of a 1.2 kbp DNA fragment into the C-terminal region of nqr6 gene.
Hayashi
M
M
Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Fujii
J
J
Unemoto
T
T
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D011808:Quinone Reductases / Q000235:genetics*; D014733:Vibrio / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C084142:sodium-translocating NADH-quinone reductase; D011808:Quinone Reductases