title: The presence of a manganese-rich particle in lysosome of rat pancreas due to excess manganese treatment.
abstract: Manganese (Mn) accumulates at a higher level in the pancreas than in any other organs when excess Mn is administered to the rat. The present study is carried out to analyze the intracellular localization of Mn existed in the pancreatic cell of Mn-treated rats. Transmission electron microscope and X-ray micro-analysis connected with a rapid freezing fixation technique showed that a large amount of Mn was localized in lysosomal particles of the pancreatic cell of Mn-treated rats. The Mn-rich particles disappeared when the element-administration was discontinued, showing that the accumulation of Mn is reversible. To confirm that Mn is in the lysosomes, a centrifugal subcellular-fractionation and a neutron activation analysis were carried out. The result indicated that much Mn existed in the lysosomal fraction.
authors.affiliation: Faculty of Pharmacy, Meijo University, Nagoya, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: R
authors.initials: R
authors.lastname: Oshima
authors.forename: K
authors.initials: K
authors.lastname: Inagaki
authors.forename: S
authors.initials: S
authors.lastname: Aita
authors.forename: H
authors.initials: H
authors.lastname: Nisioka
authors.forename: Y
authors.initials: Y
authors.lastname: Kondo
authors.forename: H
authors.initials: H
authors.lastname: Ishizuka
authors.forename: J
authors.initials: J
authors.lastname: Takada
authors.forename: M
authors.initials: M
authors.lastname: Nishida
mesh_terms: D000818:Animals; D016616:Cryoultramicrotomy; D004577:Electron Probe Microanalysis; D008247:Lysosomes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008345:Manganese / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000737:chemistry
chemical_list: D008345:Manganese