Inhibition of anchorage-independent growth of ras-transformed cells on polyHEMA surface by antisense oligodeoxynucleotides directed against K-ras.
We examined the effect of antisense oligodeoxynucleotides (AS ODNs) directed against v-Ki-ras on the anchorage-independent growth of v-Ki-ras-transformed rat fibroblasts using plates coated with an antiadhesive polymer, poly(2-hydroxyethyl methacrylate) (polyHEMA). Among AS ODNs tested, 17mer AS ODN centered around codon 12 of v-Ki-ras inhibited the v-Ki-Ras expression and v-Ki-Ras-induced anchorage-independent growth, while its sense sequence did not affect it. Using polyHEMA plates, the direct addition of AS ODNs to the cells growing anchorage-independently and various treatment schedules of AS ODNs are now available to identify the most desirable conditions for AS ODNs treatment.
Kawada
M
M
Department of Bioactive Molecules, National Institute of Health, Tokyo, Japan.
Fukazawa
H
H
Mizuno
S
S
Uehara
Y
Y
D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects*; D002472:Cell Transformation, Viral; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D011905:Genes, ras; D008722:Methods; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D011102:Polyhydroxyethyl Methacrylate; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics; D051381:Rats
D016376:Oligonucleotides, Antisense; D011102:Polyhydroxyethyl Methacrylate; D015689:Oncogene Protein p21(ras); D016283:Proto-Oncogene Proteins p21(ras)