title: Amelioration of chronic inflammation by ingestion of elemental diet in a rat model of granulomatous enteritis.
abstract: The beneficial effect of elemental diet (ED) in the treatment of Crohn's disease is reported, although the exact mechanism for this remains to be elucidated. In this study the effects of ED on intestinal inflammation were investigated in a rat model of granulomatous enteritis. Intestinal inflammation was induced by a single intramural injection of peptidoglycan-polysaccharide (PG-PS) from group A streptococci into rat ileal Peyer's patches. A single injection of PG-PS in combination with fibrinogen, which retains PG-PS at the injection site, induced severe granulomatous inflammation associated with mucosal ulceration. Immunohistochemical study and immunocytochemical analysis of the cell suspension from Peyer's patches showed accumulation of macrophages and an increase in interleukin-2 receptor (IL-2R)-positive T cells after PG-PS treatment. Chemiluminescence (ChL) activity and nitrite and nitrate (NOx) levels in the mesenteric venous blood as well as Ca(2+)-independent (inducible) nitric oxide synthase (NOS) activity in Peyer's patches were increased by PG-PS treatment. In rats fed with ED, both macroscopic and histologic damage scores were significantly decreased as compared with those in rats fed with the control diet. ED inhibited the increase in the numbers of macrophages and IL-2R-positive T cells in Peyer's patches. Increased ChL activity, NOx levels, and Ca(2+)-independent NOS activity were also reduced significantly by feeding with ED. These data suggest that ED reduces progression of PG-PS-induced chronic intestinal inflammation by modulating activation of T cells, production of nitric oxide, and generation of oxygen free radicals.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Miura
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: N
authors.initials: N
authors.lastname: Ohkubo
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuzuki
authors.forename: D
authors.initials: D
authors.lastname: Fukumura
authors.forename: H
authors.initials: H
authors.lastname: Serizawa
authors.forename: I
authors.initials: I
authors.lastname: Kurose
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Ishii
mesh_terms: D000596:Amino Acids / Q000008:administration & dosage; D000818:Animals; D003424:Crohn Disease / Q000178:diet therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D005526:Food, Formulated; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D007413:Intestinal Mucosa / Q000201:enzymology / Q000473:pathology; D008297:Male; D009566:Nitrates / Q000097:blood; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009573:Nitrites / Q000097:blood; D010457:Peptidoglycan; D010581:Peyer's Patches / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013297:Streptococcus pyogenes
chemical_list: D000596:Amino Acids; D009566:Nitrates; D009573:Nitrites; D010457:Peptidoglycan; D019001:Nitric Oxide Synthase