Long-term stability of albumin, protein HC, immunoglobulin G, kappa- and lambda-chain-immunoreactivity, orosomucoid and alpha 1-antitrypsin in urine stored at -20 degrees C.
The stability of albumin, protein HC, immunoglobulin G, kappa- and lambda-chain immunoreactivity, orosomucoid and alpha 1-antitrypsin in urine stored at -20 degrees C for up to 24 months was investigated. Significant decreases of the median concentration values for protein HC, IgG and alpha 1-antitrypsin were observed for native urine. Addition to urine of a preservative solution containing benzamidinium chloride, EDTA, tris(hydroxymethyl)-aminomethane and azide prevented the decreases of the concentration values for protein HC and IgG but not for alpha 1-antitrypsin. The median concentration values for albumin, orosomucoid and kappa- and lambda-chain immunoreactivity did not change significantly upon storage of native urine, nor for urine with the preservative solution.
Tencer
J
J
Department of Nephrology, Lund University Hospital, Sweden.
Thysell
H
H
Andersson
K
K
Grubb
A
A
D000419:Albuminuria / Q000652:urine*; D000510:Alpha-Globulins / Q000652:urine*; D015925:Cryopreservation; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine*; D007145:Immunoglobulin kappa-Chains / Q000652:urine*; D007146:Immunoglobulin lambda-Chains / Q000652:urine*; D007674:Kidney Diseases / Q000175:diagnosis / Q000652:urine; D009961:Orosomucoid / Q000652:urine*; D013048:Specimen Handling; D013997:Time Factors; D000515:alpha 1-Antitrypsin / Q000652:urine*
D000510:Alpha-Globulins; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D009961:Orosomucoid; D000515:alpha 1-Antitrypsin; C016001:alpha-1-microglobulin