title: Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution.
abstract: A simple method for the isolation of alpha-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen alpha-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (> 95%) and purity (> 98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods e.g. chromatographic procedures.
authors.affiliation: Institut für Medizinische Molekularbiologie, Medizinische, Universität zu Lübeck, Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Açil
authors.forename: J
authors.initials: J
authors.lastname: Brinckmann
authors.forename: P
authors.initials: P
authors.lastname: Behrens
authors.forename: P K
authors.initials: PK
authors.lastname: Müller
authors.forename: B
authors.initials: B
authors.lastname: Bätge
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006901:Hydroxylysine / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D010434:Pepsin A / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013552:Swine
chemical_list: D000596:Amino Acids; D006901:Hydroxylysine; D012967:Sodium Dodecyl Sulfate; D003094:Collagen; D010434:Pepsin A; D006909:Hydroxyproline