Augmented expression of the endothelin-A receptor gene in cultured mesangial cells from stroke-prone spontaneously hypertensive rats.
1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET-A receptor (ET-AR) gene and the ET-B receptor (ET-BR) gene in cultured mesangial cells isolated from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET-AR predominantly. The level of the ET-AR mRNA in mesangial cells from SHRSP was 5-fold higher than that in the cells from WKY. The ET-BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET-AR gene was markedly augmented in mesangial cells from SHRSP.
Hiraoka
J
J
Graduate School of Human and Environmental Studies, Kyoto University, Japan.
Arai
H
H
Yoshimasa
T
T
Takaya
K
K
Miyamoto
Y
Y
Yamashita
J
J
Suga
S
S
Ogawa
Y
Y
Shirakami
G
G
Itoh
H
H
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D002561:Cerebrovascular Disorders / Q000235:genetics / Q000503:physiopathology; D005920:Glomerular Mesangium / Q000378:metabolism*; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017466:Receptors, Endothelin / Q000096:biosynthesis* / Q000235:genetics*
D017466:Receptors, Endothelin