title: Plasma glutathione S-transferase alpha 1-1 levels in patients with chronic liver disorders.
abstract: Recent data suggest that plasma levels of the phase II detoxification enzyme glutathione S-transferase alpha may be a sensitive indicator of hepatocellular integrity in acute liver disorders but little information is available in chronic hepatic disorders. Using a newly developed enzyme linked immunosorbent assay, glutathione S-transferase A1-1 (GSTA1-1) levels were measured in 279 plasma samples from patients with chronic liver disorders. Results were categorized as normal or elevated plasma GSTA1-1 and normal or elevated plasma aspartate aminotransferase (AST) levels. In 24 patients with alcoholic liver cirrhosis, plasma GSTA1-1 levels were not significantly different from a group of 350 healthy controls and only one patient (4%) had an elevated GSTA1-1 level while 10 (42%) patients had elevated AST activities. In samples from patients with primary biliary cirrhosis (n = 150), primary sclerosing cholangitis (n = 26) or chronic hepatitis (n = 79) significantly (P < 0.0001) elevated plasma GSTA1-1 concentrations were detected in 25 (17%), 7 (27%) and 17 (22%) of the samples, respectively. AST activities were increased in a higher percentage of samples in all three disorders: 89%, 88%, and 57%, respectively. Plasma GSTA1-1 and AST levels were significantly correlated (P < 0.005) in the above mentioned disorders but not in alcoholic liver cirrhosis. It is concluded that plasma GSTA1-1 is not a sensitive parameter for the detection of hepatocellular damage in chronic liver disorders.
authors.affiliation: Department of Gastroenterology and Hepatology, University Hospital St. Radboud, Njimegen, The Netherlands. T.Mulder@gastro.azn.nl
authors.forename: T P
authors.initials: TP
authors.lastname: Mulder
authors.forename: A R
authors.initials: AR
authors.lastname: Janssens
authors.forename: W C
authors.initials: WC
authors.lastname: de Bruin
authors.forename: W H
authors.initials: WH
authors.lastname: Peters
authors.forename: M P
authors.initials: MP
authors.lastname: Cooreman
authors.forename: J B
authors.initials: JB
authors.lastname: Jansen
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D002908:Chronic Disease; D005982:Glutathione Transferase / Q000097:blood*; D006801:Humans; D008107:Liver Diseases / Q000097:blood* / Q000201:enzymology*
chemical_list: D005982:Glutathione Transferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase