title: Lectin histochemistry during in vitro capacitation and acrosome reaction in boar spermatozoa: new lectins for evaluating acrosomal status of boar spermatozoa.
abstract: Six peroxidase (HRP)-conjugated lectins have been used to analyze the distribution of carbohydrates in ejaculated boar spermatozoa during in vitro capacitation and acrosome reaction in air-dried preparations. The membranes of boar spermatozoa were positive to WGA and Con-A in the acrosome, middle piece and principal piece. PNA labelled the acrosomal region. DBA, UEA-I and LTA bound weakly or not to the spermatozoa. The lectin labelling pattern did not change during the in vitro capacitation. A diminution in the proportion of spermatozoa with acrosome positive to lectins PNA, WGA and Con-A was observed after coincubation with homologous immature oocytes. A positive correlation existed between the percentages of lectins bound to spermatozoa and the triple stain technique. The results show that peroxidase-conjugated lectins may be useful in determining acrosomal integrity of boar spermatozoa during in vitro capacitation and acrosome reaction.
authors.affiliation: Department of Animal Pathology, Reproduction and Obstetrics, Veterinary Faculty, University of Murcia, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Vazquez
authors.forename: E
authors.initials: E
authors.lastname: Martinez
authors.forename: L M
authors.initials: LM
authors.lastname: Pastor
authors.forename: J
authors.initials: J
authors.lastname: Roca
authors.forename: C
authors.initials: C
authors.lastname: Matas
authors.forename: A
authors.initials: A
authors.lastname: Calvo
mesh_terms: D000177:Acrosome / Q000648:ultrastructure*; D000818:Animals; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000378:metabolism; D004396:Coloring Agents; D005307:Fertilization in Vitro; D006651:Histocytochemistry; D066298:In Vitro Techniques; D037102:Lectins; D008297:Male; D013075:Sperm Capacitation / Q000502:physiology*; D013094:Spermatozoa / Q000648:ultrastructure*; D013552:Swine
chemical_list: D004396:Coloring Agents; D037102:Lectins