title: Characterization of a mutant of Vibrio vulnificus for heme utilization.
abstract: Vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. This process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin IX). In the present study, we isolated and characterized a mutant for protoheme utilization. One mutant isolated by treatment with a chemical mutagen was shown to be unable to use either protoheme or heme proteins, but multiplied in a medium supplemented with an iron siderophore, such as iron-vulnibactin. Like a wild-type strain, the mutant sensed iron depletion, so that the 74- and 79-kDa outer membrane proteins were expressed under iron-regulated conditions. Both the parent and mutant strains secreted hemolysin independent of the iron concentration of the medium. Whole cells of either of the strains were equally capable of binding of hematin. Taken together, the data suggest that the mutant may have a mutation in a gene encoding an inner membrane or a periplasmic protein which transports protoheme or iron dissociated from protoporphyrin IX into the cell.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University, Japan. miyoshi@pheasant.pharm.okayama-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Miyoshi
authors.forename: Y
authors.initials: Y
authors.lastname: Inami
authors.forename: Y
authors.initials: Y
authors.lastname: Moriya
authors.forename: T
authors.initials: T
authors.lastname: Kamei
authors.forename: M M
authors.initials: MM
authors.lastname: Rahman
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Tomochika
authors.forename: S
authors.initials: S
authors.lastname: Shinoda
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis; D006427:Hemin / Q000378:metabolism* / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000378:metabolism; D009154:Mutation / Q000502:physiology; D011485:Protein Binding / Q000502:physiology; D014733:Vibrio / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D006427:Hemin