Serological characterization of Actinobacillus pleuropneumoniae biotype 2 strains isolated from pigs in two Danish herds.
Eight Actinobacillus pleuropneumoniae biotype 2 strains were isolated in pure culture from lungs of pigs originating from two Danish herds with growing and finishing pigs. The antigenic properties were studied by indirect haemagglutination (IHA) and immunodiffusion (ID) tests using soluble surface antigens and by enzyme-linked immunosorbent assays (ELISA) using capsular enriched fractions and LPS. In all tests the strains proved antigenically homogeneous and serologically distinct from the known biotype 1 and 2 serotypes. Thus, the strains represent a new serotype which is provisionally proposed as biotype 2 serotype 14.
Nielsen
R
R
Danish Veterinary Laboratory, Copenhagen V, Denmark.
Andresen
L O
LO
Plambeck
T
T
Nielsen
J P
JP
Krarup
L T
LT
Jorsal
S E
SE
D000189:Actinobacillus Infections / Q000145:classification / Q000175:diagnosis / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000302:isolation & purification; D000818:Animals; D003168:Complement Fixation Tests; D003718:Denmark; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D015151:Immunoblotting; D005779:Immunodiffusion; D007841:Latex Fixation Tests; D008070:Lipopolysaccharides / Q000032:analysis; D008168:Lung / Q000382:microbiology*; D011001:Pleuropneumonia / Q000145:classification / Q000175:diagnosis / Q000662:veterinary*; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases
D008070:Lipopolysaccharides