title: Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenemia assay.
abstract: Quantitative cytomegalovirus antigenemia and DNAemia were determined in peripheral leukocytes of 25 patients stored for up to 72 h at room temperature (RT) and 4 degrees C before processing. Numbers of antigen-positive cells significantly decreased with time. The decline was greater at RT than at 4 degrees C. In contrast, no significant alterations in DNAemia occurred.
authors.affiliation: Institut für Medizinische Mikrobiologie und Immunologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Schäfer
authors.forename: W
authors.initials: W
authors.lastname: Tenschert
authors.forename: K
authors.initials: K
authors.lastname: Gutensohn
authors.forename: R
authors.initials: R
authors.lastname: Laufs
mesh_terms: D000956:Antigens, Viral / Q000097:blood*; D001800:Blood Specimen Collection; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005914:Globins / Q000235:genetics; D006801:Humans; D007962:Leukocytes / Q000821:virology; D010750:Phosphoproteins / Q000097:blood / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014763:Viral Matrix Proteins / Q000097:blood / Q000276:immunology; D014773:Virology / Q000379:methods
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D005914:Globins