Both E12 and E47 allow commitment to the B cell lineage.
The E2A gene products, E12 and E47, are required for proper B cell development. Mice lacking the E2A gene products generate only a very small number of B220+ cells, which lack immunoglobulin DJ(H) rearrangements. We have now generated mice expressing either E12 or E47. B cell development in mice expressing E12 but lacking E47 is perturbed at the pro-B cell stage, and these mice lack IgM+B220+ B cells in both bone marrow and spleen. IgM+B220+ B cells can be detected, albeit at significantly reduced levels, in the bone marrow and spleen of mice lacking E12. Ectopic expression of both E12 and E47 in a null mutant background shows that E12 and E47 act in concert to promote B lineage development. Taken together, the data indicate that both E12 and E47 allow commitment to the B cell lineage and act synergistically to promote B lymphocyte maturation.
Bain
G
G
Department of Biology, University of California, San Diego, La Jolla 92093, USA.
Robanus Maandag
E C
EC
te Riele
H P
HP
Feeney
A J
AJ
Sheehy
A
A
Schlissel
M
M
Shinton
S A
SA
Hardy
R R
RR
Murre
C
C
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D004357:Drug Synergism; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D051820:TCF Transcription Factors; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D014158:Transcription, Genetic / Q000235:genetics / Q000276:immunology
D004268:DNA-Binding Proteins; D051820:TCF Transcription Factors; C544913:Tcf7l1 protein, mouse; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors