Identification and analysis of a lytic-phase origin of DNA replication in human herpesvirus 7.
Human herpesvirus 7 (HHV-7) DNA sequences colinear with the HHV-6 lytic-phase origin of DNA replication (oriLyt) were amplified by PCR. Plasmid constructs containing these sequences were replicated in HHV-7-infected cord blood mononuclear cells but not in HHV-6-infected cells. In contrast, plasmids bearing HHV-6 oriLyt were replicated in both HHV-6- and HHV-7-infected cells. Finally, the minimal HHV-7 DNA element necessary for replicator activity was mapped to a 600-bp region which contains two sites with high homology to the consensus binding site for the HHV-6 origin binding protein. At least one of these binding sites was shown to be essential for replicator function of HHV-7 oriLyt.
van Loon
N
N
Department of Microbiology and Immunology, Medical Center, University of Rochester, New York 14642, USA.
Dykes
C
C
Deng
H
H
Dominguez
G
G
Nicholas
J
J
Dewhurst
S
S
D001483:Base Sequence; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000502:physiology; D016199:Herpesvirus 7, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D018741:Replication Origin; D014779:Virus Replication
D004279:DNA, Viral