Measurement of glucose and water transport in the human duodenum in vivo using a dialysis bag.
OBJECTIVE
Only a few studies have evaluated duodenal absorptive or secretory function in humans. In the present study we determined duodenal glucose and water transport in humans in vivo.


METHODS
Duodenal glucose and water transport were studied in 27 healthy volunteers using a modification of the dialysis bag technique for measuring rectal water and sodium transport.


RESULTS
Net glucose absorption increased progressively over the range of glucose concentrations studied (10 mM to 100 mM) from 0.21 +/- 0.19 to 1.76 +/- 0.15 mM/cm2/90 min. Maximum water absorption occurred from the 10 mM glucose solution (35.87 +/- 7.5 microliters/cm2/90 min) and at a significantly greater rate than from the 80 mM glucose (11.60 +/- 4.0 microliters/cm2/90 min) and the 100 mM glucose (14.90 +/- 1.7 microliters/cm2/90 min solution.


CONCLUSIONS
This study demonstrates that glucose and water are absorbed by the human duodenum in vivo and that the dialysis bag technique can be adapted to measure transport processes in areas of the intestine other than the rectum.
Rolston
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Department of Gastroenterology, Christian Medical College and Hospital, Vellore, India.
D000328:Adult; D001692:Biological Transport; D001834:Body Water / Q000378:metabolism*; D003956:Dialysis / Q000295:instrumentation; D004386:Duodenum / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D008297:Male; D009994:Osmolar Concentration
D005947:Glucose