title: Autoantibodies, immunoglobulins, complement and circulating immune complexes in acute malaria.
abstract: BACKGROUND
  Malaria caused by Plasmodium vivax and Plasmodium falciparum is common in the Indian subcontinent. Studies conducted elsewhere have suggested that malarial infection causes intense immunostimulation. We screened patients with malarial infection for autoantibodies and measured the immunoglobulin, circulating immune complex and complement levels to determine the extent of immunological alterations in these patients.
  
  
  METHODS
  One hundred adults with acute malarial infection confirmed by examination of the peripheral blood smear and 25 age- and sex-matched controls were studied. An autoantibody screen and serum immunoglobulin complement (C3 and C4) and circulating immune complex levels were measured at the time of admission and 4 weeks after they became afebrile. A direct Coomb's test was also done.
  
  
  RESULTS
  Anti-ssDNA, anti-dsDNA and rheumatoid factor were positive at the time of admission in 51, 30 and 38 patients respectively. None of the controls were positive for these autoantibodies except for one who was positive for rheumatoid factor. The IgM, IgG and IgA levels were raised in 16, 25 and 36 patients respectively. Circulating immune complex levels were raised in 32 patients and complement C3 and C4 were low in 8 and 31 patients. Follow up studies at 4 weeks in 19 patients showed that the autoantibodies were negative. However, the immunoglobulin, C4 and circulating immune complex levels remained elevated. Six per cent of patients had a positive direct Coomb's test with reticulocytosis at the time of presentation.
  
  
  CONCLUSIONS
  Acute malarial infection can cause false-positive results for anti-ssDNA, anti-dsDNA and rheumatoid factor and may also cause a rise in the serum immunoglobulin, complement and circulating immune complex levels.
authors.affiliation: Government Medical College and New Civil Hospital, Surat, Gujarat, India.
authors.forename: K N
authors.initials: KN
authors.lastname: Jhaveri
authors.forename: K
authors.initials: K
authors.lastname: Ghosh
authors.forename: D
authors.initials: D
authors.lastname: Mohanty
authors.forename: B D
authors.initials: BD
authors.lastname: Parmar
authors.forename: R R
authors.initials: RR
authors.lastname: Surati
authors.forename: H M
authors.initials: HM
authors.lastname: Camoens
authors.forename: S H
authors.initials: SH
authors.lastname: Joshi
authors.forename: Y S
authors.initials: YS
authors.lastname: Iyer
authors.forename: A
authors.initials: A
authors.lastname: Desai
authors.forename: S S
authors.initials: SS
authors.lastname: Badakere
mesh_terms: D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis*; D003165:Complement System Proteins / Q000032:analysis*; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007194:India; D016778:Malaria, Falciparum / Q000276:immunology*; D016780:Malaria, Vivax / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D007136:Immunoglobulins; D003165:Complement System Proteins