Effect of alpha-tocopherol on isoproterenol induced myocardial infarction in rats--electrocardiographic biochemical and histological evidences.
The effect of alpha-tocopherol (6 mg/100 g body wt, orally, daily for 90 days) pretreatment in isoproterenol (20 mg/100 g body wt, subcutaneously, twice at an interval of two days at the end of the alpha-tocopherol pretreatment) induced myocardial infarction was studied in rats. Isoproterenol administered rats showed electrocardiographic changes suggestive of myocardial infarction with marked ST segment elevation, Q waves appearance and a significant increase in heart rate. In isoproterenol administered rats, a significant decrease was observed in the activities of marker enzymes such as aspartate amino transferase, alanine amino transferase, lactate dehydrogenase and creatine kinase in heart and aorta with a significant increase in their activities in serum. The levels of lipid peroxides in terms of "TBA reactants" increased significantly in serum, heart and aorta on isoproterenol administration. The histology of heart and aorta showed marked fragmentation of muscle fibres and necrotic lesions in isoproterenol administered rats. alpha-Tocopherol pretreated rats showed a near normal ECG pattern, levels of lipid peroxides, activities of marker enzymes and a near normal histology of heart and aorta on isoproterenol administration.
Ithayarasi
A P
AP
Department of Biochemistry, University of Madras.
Padmavathy
V N
VN
Shyamala Devi
C
C
D000818:Animals; D001011:Aorta / Q000201:enzymology; D002316:Cardiotonic Agents / Q000633:toxicity*; D004347:Drug Interactions; D004562:Electrocardiography; D006339:Heart Rate / Q000187:drug effects; D007545:Isoproterenol / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000097:blood / Q000378:metabolism; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced* / Q000503:physiopathology / Q000517:prevention & control*; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000627:therapeutic use*
D002316:Cardiotonic Agents; D008054:Lipid Peroxides; D014810:Vitamin E; D007545:Isoproterenol