title: Local accumulation of alpha-spectrin-related protein under plasma membrane during capping and phagocytosis in Acanthamoeba.
abstract: During capping and phagocytosis the interaction between cluster cell surface receptors and the submembraneous actin-based skeleton may be mediated by spectrin-like proteins. To test this possibility we examined the localization of an alpha-spectrin immunoanalogue, that had been previously identified in whole extracts of Acanthamoeba, during capping of Con A receptors and during phagocytosis of Con A-coated yeast. During capping alpha-spectrin and filamentous actin co-migrated with the Con A receptors and accumulated in the region of cap formation, as demonstrated by double immunofluorescence studies. Immunoelectron microscopy revealed submembraneous location of alpha-spectrin in cells exposed to Con A, both at the time of initial cross-linking and during accumulation of alpha-spectrin in the region of the cap. Phagocytosis studies showed that alpha-spectrin and actin filaments were concentrated around phagocytic cups that enclosed ConA-coated yeast upon internalization. The proteins also surrounded nascent phagosomes present in the vicinity of the plasma membrane but were absent at the later time point of phagosome maturation. These data demonstrate a correlation between clustering of cell surface receptors and submembraneous localization of alpha-spectrin, suggesting an involvement of spectrin-like proteins in mediating the interaction of receptor clusters with the actin cytoskeleton.
authors.affiliation: Nencki Institute of Experimental Biology, Department of Cell Biology, Warsaw, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Kwiatkowska
authors.forename: A
authors.initials: A
authors.lastname: Sobota
mesh_terms: D000048:Acanthamoeba / Q000378:metabolism* / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D010587:Phagocytosis; D011506:Proteins / Q000378:metabolism; D011940:Receptor Aggregation; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D013049:Spectrin / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D000199:Actins; D011506:Proteins; D011952:Receptors, Concanavalin A; D013049:Spectrin