title: Evaluation of a new measurement procedure for the concentration of ferritin in serum.
abstract: The aim of this study was the evaluation of an immunoturbidimetric measurement procedure for the concentration of ferritin in serum, based on the new Tina-quant Ferritin reagents kit (Boehringer Mannheim) in which the antibody-coated latex particles have been modified with regard to the previous Tina-quant Ferritin reagents kit. The evaluation was carried out using the BM/Hitachi 917 Analyzer. The evaluation included the within-run and between-day imprecisions estimation, the comparison of measurement procedures and the assessment of the measuring range. An additional study of between-day imprecision was done using 4 microl and 20 microl of sample. Detection limit was studied using BM/Hitachi 917 (4 microl and 20 microl of sample), ES 300 and Cobas-Core analyzers. The coefficients of variation obtained in the between-day imprecision study are lower when 20 microl of sample is used instead of 4 microl. In the measurement procedure using 20 microl, the coefficient of variation observed at physiological concentrations is 2.4%. The detection limit with 20 microl of sample is lower than with 4 microl. Therefore, in ferropenic anaemia studies it is convenient to use the measurement procedure with 20 microl sample volumes, since it possesses the best metrological characteristics for this purpose.
authors.affiliation: Servei de Bioquímica Clínica, Ciutat Sanitària i Universitària de Bellvitge, Barcelona, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Palencia-Domínguez
authors.forename: J M
authors.initials: JM
authors.lastname: Martínez-Cervera
authors.forename: X
authors.initials: X
authors.lastname: Fuentes-Arderiu
mesh_terms: D005069:Evaluation Studies as Topic; D005293:Ferritins / Q000097:blood*; D006801:Humans; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic; D005293:Ferritins