Molecular cloning and nucleotide sequencing of bioF (7-keto-8-amino pelargonic acid synthetase), bioC and bioD (dethiobiotin synthetase) genes of Erwinia herbicola.
The biotin operon of Erwinia herbicola Eho 10 was cloned and characterized by complementation of E. coli biotin mutants. The operon was found to contain five genes arranged in the order, bioABFCD. The nucleotide sequences of bioF (7-keto-8-aminopelargonic acid synthetase), bioC and bioD (dethiobiotin synthetase) were determined and analyzed. The nucleotide sequences and deduced amino acid sequences of bioFCD were compared with the corresponding sequences from Escherichia coli, Bacillus sphaericus, Serratia marcescens and Brevibacterium flavum.
Wu
C H
CH
Department of Biology, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC.
Bao
Y Y
YY
Shao
C P
CP
Shiuan
D
D
D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001710:Biotin / Q000235:genetics*; D019731:Carbon-Nitrogen Ligases; D004885:Erwinia / Q000201:enzymology* / Q000235:genetics*; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D017385:Sequence Homology
D001710:Biotin; D000217:Acyltransferases; C021929:8-amino-7-oxononanoate synthase; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C020024:dethiobiotin synthetase