[The use of the polymerase chain reaction in analyzing ancient DNAs (exemplified by that of the Enmynveem mammoth)].
DNA was isolated from the Enmynveem mammoth muscles, and the control region and cytochrome b gene of the mitochondrial genome were analyzed by polymerase chain reaction (PCR). The mammoth DNA was amplified by both the classical PCR (two primer system) and the single-primer PCR (spPCR) resulting in DNA fragments up to 1600 bp long. Restriction analysis of the mitochondrial cytochrome b gene was carried out. Cytochromes b genes in three Elephantidae genera were compared.
Maliarchuk
B A
BA
Derevenko
M V
MV
Lapinskiĭ
A G
AG
Solovenchuk
L L
LL
D000818:Animals; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics; D004606:Elephants / Q000235:genetics*; D008969:Molecular Sequence Data; D010163:Paleontology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping / Q000379:methods; D012800:Siberia
D003573:Cytochrome b Group; D017931:DNA Primers; D004272:DNA, Mitochondrial; D004247:DNA
Ispol'zovanie polimeraznoĭ tsepnoĭ reaktsii v analize drevnikh DNK (na primere Enmynveemskogo mamonta).