Phototoxicity to sulphonamide derived oral antidiabetics and diuretics. Comparative in vitro and in vivo investigations.
The oral antidiabetics chlorpropamide, glibenclamide, glipizide, gliquiudone, glymidine, tolazamide and tolbutamide, and the diuretics bemetizide, bendroflumethiazide, benzylhydrochlorothiazide, bumetanide, butizide, chlortalidone, furosemide, hydrochlorothiazide, hydroflumethiazide, indapamide, piretanide, polythiazide, trichlormethiazide, and xipamide were investigated for potential phototoxicity in vitro using a cell culture model, and in vivo in hairless mice. After exposure to broad band UVA, the majority of the substances tested in vitro yielded a phototoxic action leading to loss of culture forming ability. In vivo, all tested substances induced edema or ulceration, and lead to a significantly increase in skin fold thickness of the mouse skin. In all, a number of substances not described to induce clinical photosensitivity nor phototoxicity in vitro or in vivo were detected in our testing. When determining potential photosensitizers, it seems important to utilize different test methods, as not all substances will exhibit action in a given assay.
Selvaag
E
E
Department of Dermatology, Ullevaal Hospital, University of Oslo, Norway.
Anholt
H
H
Moan
J
J
Thune
P
P
D000284:Administration, Oral; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004232:Diuretics / Q000528:radiation effects / Q000633:toxicity*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007004:Hypoglycemic Agents / Q000528:radiation effects / Q000633:toxicity*; D008027:Light; D051379:Mice; D008812:Mice, Hairless; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000528:radiation effects / Q000633:toxicity*; D013449:Sulfonamides / Q000528:radiation effects / Q000633:toxicity*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D002583:Uterine Cervical Neoplasms
D004232:Diuretics; D007004:Hypoglycemic Agents; D017319:Photosensitizing Agents; D013449:Sulfonamides; D004121:Dimethyl Sulfoxide