The contribution of peripheral bradykinin B2 receptors to carrageenan-evoked oedema and spinal c-Fos expression in rats.
Intraplantar co-injection of HOE140 (D-Arg-[Hyp3,Thi5,D-Tic7,Oic8]bradykinin), a selective bradykinin B2 receptor antagonist (0.1, 1 and 10 micrograms), with carrageenan dose-dependently (r = 0.66, P < 0.01) reduced the carrageenan-evoked total number of c-Fos protein-like immunoreactive (c-Fos-LI) neurones (23 +/- 5%, 35 +/- 6% and 50 +/- 5% reduction; P < 0.01, P < 0.001 and P < 0.001, respectively). These reducing effects were dose-dependent for the number of c-Fos-LI neurones in both superficial (r = 0.70, P < 0.01) and deep (r = 0.53, P < 0.05) laminae. Intraplantar co-injection of HOE140 (0.1, 1 and 10 micrograms) with carrageenan significantly reduced the carrageenan-evoked paw (25 +/- 7%, 41 +/- 6% and 41 +/- 3% reduction; P < 0.001 for all) and ankle (46 +/- 6%, 61 +/- 5% and 61 +/- 5% reduction; P < 0.001 for all) oedema. Our results provide further evidence for the involvement of peripheral bradykinin B2 receptors in carrageenan-induced inflammatory nociceptive transmission.
Buritova
J
J
Unité de Recherches de Physiopharmacologie du Système Nerveux, INSERM U.161, Paris, France. buritova@broca.inserm.fr
Chapman
V
V
Honoré
P
P
Besson
J M
JM
D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000494:pharmacology; D065168:Bradykinin Receptor Antagonists; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D005528:Foot / Q000473:pathology; D006614:Hindlimb / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D043782:Receptor, Bradykinin B2; D013116:Spinal Cord / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*
D065168:Bradykinin Receptor Antagonists; D016760:Proto-Oncogene Proteins c-fos; D043782:Receptor, Bradykinin B2; C065679:icatibant; D002351:Carrageenan; D001920:Bradykinin