title: Expression of tissue inhibitor of metalloproteinase-1 and type IV collagenase/gelatinase messenger RNAs in human breast cancer.
abstract: The relationship between malignant epithelial cell growth and the formation of tumor stroma is poorly understood. To investigate the roles of type IV collagenases/gelatinases and tissue inhibitor of metalloproteinase-1 (TIMP-1) in human breast cancer, we localized their messenger RNAs (mRNAs) in normal and malignant breast tissue. Type IV collagenase/gelatinase mRNAs were expressed at low levels by some tumor cells. High levels of TIMP-1 mRNA were found in all areas where tissue remodeling was evident, particularly in cells at the tumor-stromal interface. Small blood vessels in both the tumor and stroma were often outlined by high levels of TIMP-1 mRNA. These results indicate that TIMP-1 and type IV collagenases/gelatinases are expressed independently in breast cancer.
authors.affiliation: Laboratory of Cellular Carcinogenesis and Tumor Promotion, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: C K
authors.initials: CK
authors.lastname: Lindsay
authors.forename: U P
authors.initials: UP
authors.lastname: Thorgeirsson
authors.forename: H
authors.initials: H
authors.lastname: Tsuda
authors.forename: S
authors.initials: S
authors.lastname: Hirohashi
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D001943:Breast Neoplasms / Q000098:blood supply / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017364:Collagenases / Q000235:genetics* / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000378:metabolism; D011480:Protease Inhibitors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D018952:Antigens, CD34; D006023:Glycoproteins; D011480:Protease Inhibitors; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9