Clostridium perfringens toxins absorbed on filter paper--a simple method for sample conservation.
Clostridium perfringens type C and type D toxins were absorbed on filter paper, dried and stored at room temperature (18-20 degrees C), at 37 degrees C, at 4 degrees C and at -20 degrees C. Type specific toxin was correctly identified in the EIA for 74 days. Absorption on filter paper may offer a simple method for conservation and transport of post mortem samples from cases of suspected enterotoxaemia.
Röhner
E
E
Institute of Animal Hygiene and Environmental Hygiene, Freie Universität Berlin.
Younan
M
M
Müller
W
W
D000042:Absorption; D000818:Animals; D000829:Animals, Domestic; D001344:Autopsy; D001427:Bacterial Toxins / Q000032:analysis*; D002788:Cholesterol Esters / Q000032:analysis*; D003015:Clostridium Infections / Q000473:pathology / Q000662:veterinary; D003016:Clostridium perfringens; D003470:Culture Media; D019446:Endotoxemia / Q000473:pathology / Q000662:veterinary; D007124:Immunoenzyme Techniques; D010209:Paper; D011726:Pyridinium Compounds / Q000032:analysis*; D013048:Specimen Handling / Q000379:methods
D001427:Bacterial Toxins; C031884:CPB protein, Clostridium perfringens; D002788:Cholesterol Esters; D003470:Culture Media; D011726:Pyridinium Compounds; C075403:cholesterylphosphorylethylpyridinium