Determination of furosine in hair by liquid chromatography-tandem mass spectrometry.
A sensitive and reliable analytical method for determining furosine in hair has been developed using liquid chromatography-tandem mass spectrometry. Fructose-N(omega)-formyl-d4-DL-lysine was synthesized and used as an internal standard. By the present method, glycated lysine levels in hair could be determined as fructose-lysine in only 0.1 mg of hair sample, ranging from 35.1-72.6 ng/mg hair among five healthy volunteers, which corresponded to 0.0635-0.153% of the total lysine contents in hair determined by amino acid analysis.
Takemura
A
A
Product Development Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
Muramatsu
S
S
Kobayashi
N
N
Nakagawa
A
A
Kitazawa
E
E
Uematsu
T
T
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005632:Fructose / Q000032:analysis; D006031:Glycosylation; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D011786:Quality Control; D012680:Sensitivity and Specificity
C018948:furosine; D005632:Fructose; D008239:Lysine