Noradrenaline inhibits the programmed cell death induced by 1,25-dihydroxyvitamin D3 in glioma.
The rat glioma cell line C6.9 has been recently reported to respond to 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) by the induction of a programmed cell death. Since, in vivo, glial cells are thought to be exposed to several neurotransmitters, we investigated the possibility of a neurotransmitter-mediated inhibition of this active cell death process. Noradrenaline and the beta-adrenoceptor agonist isoproterenol showed significant inhibition of the 1,25(OH)2D3-induced programmed cell death. The beta-adrenoceptor antagonist propanolol reversed this inhibition, while the alpha-adrenoceptor antagonist yohimbin was devoid of any effect. This suggests that the efficiency of antiproliferative vitamin D-related therapies could be influenced by endogenous levels of noradrenaline.
Canova
C
C
INSERM U 298, Centre Hospitalier Universitaire, Angers, France.
Baudet
C
C
Chevalier
G
G
Brachet
P
P
Wion
D
D
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000473:pathology*; D002117:Calcitriol / Q000037:antagonists & inhibitors* / Q000633:toxicity; D053938:DNA Fragmentation / Q000187:drug effects; D005910:Glioma / Q000473:pathology*; D007545:Isoproterenol / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D014407:Tumor Cells, Cultured
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D018377:Neurotransmitter Agents; D011433:Propranolol; D002117:Calcitriol; D007545:Isoproterenol; D009638:Norepinephrine