[Feasibility of obtaining a highly active mutant of Streptomyces hygroscopicus SU-A5 using induced mutagenesis].
Mutagenic and lethal effects of gamma-radiation, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquanidine-1-oxide were investigated on spore suspensions of Streptomyces hygroscopicus SU-A5. It was shown that all the agents induced high percentage of plus mutants. The most stable and active ones were induced by gamma-radiation. The frequency of the plus mutants and the variation coefficient were used as the criteria of the mutagen efficiency. The successive exposure to gamma-radiation, MNNG and gamma-radiation resulted in the formation of a mutant with the activity 3 times higher than that of the parent strain.
Kenarova
A
A
Balutsov
V
V
Ali
K
K
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D005240:Feasibility Studies; D005720:Gamma Rays; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D016296:Mutagenesis; D009153:Mutagens / Q000633:toxicity*; D012641:Selection, Genetic; D013171:Spores, Bacterial / Q000187:drug effects / Q000528:radiation effects; D013302:Streptomyces / Q000235:genetics* / Q000302:isolation & purification
D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide
Issledovanie vozmozhnosti polucheniia vysokoaktivnogo mutanta Streptomyces hygroscopicus SU-A5 putem indutsirovannogo mutageneza.