Identification of the glycosylation site of a major soybean allergen, Gly m Bd 30K.
A major soybean allergen, Gly m Bd 30K, is a glycoprotein. A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrolyzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn170 residue. Furthermore, the sugar moiety of the allergen and peptide was shown to consist of mannose, N-acetylglucosamine, fucose, and xylose at a molar ratio of 3 : 2 : 1 : 1. These results indicate that Gly m Bd 30 K is a N-linked glycoprotein.
Bando
N
N
Department of Nutrition, School of Medicine, The University of Tokushima, Japan.
Tsuji
H
H
Yamanishi
R
R
Nio
N
N
Ogawa
T
T
D000485:Allergens / Q000032:analysis* / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism; D006031:Glycosylation; D008969:Molecular Sequence Data; D013025:Glycine max / Q000737:chemistry* / Q000276:immunology
D000485:Allergens; D006023:Glycoproteins