Physical map and cosmid contig encompassing a new interstitial deletion of the X-linked lymphoproliferative syndrome region.
The X-linked lymphoproliferative syndrome (XLP) is an inherited immuno-deficiency to Epstein-Barr virus infection that has been mapped to chromosome Xq25. Molecular analysis of XLP patients from ten different families identified a small interstitial constitutional deletion in 1 patient (XLP-D). This deletion, initially defined by a single marker, DF83, known to map to interval Xq24-q26.1, is nested within a previously reported and much larger deletion in another XLP patient (XLP-739). A cosmid minilibrary was constructed from a single mega-YAC and used to establish a contig encompassing the whole XLP-D deletion and a portion of the XLP-739 deletion. Based on this contig, the size of the XLP-D deletion can be estimated at 130 kb. The identification of this minimal deletion, within which at least a portion of the XLP gene is likely to reside, should greatly facilitate efforts in isolating the gene.
Lamartine
J
J
International Agency for Research on Cancer, Lyon, France.
Nichols
K E
KE
Yin
L
L
Krainer
M
M
Heitzmann
F
F
Bernard
A
A
Gaudi
S
S
Lenoir
G M
GM
Sullivan
J L
JL
Ikeda
J E
JE
Porta
G
G
Schlessinger
D
D
Romeo
G
G
Haber
D A
DA
Sylla
B S
BS
Harkin
D P
DP
D000293:Adolescent; D002460:Cell Line; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D003360:Cosmids; D017353:Gene Deletion; D008040:Genetic Linkage; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000235:genetics*; D008297:Male; D015183:Restriction Mapping; D013577:Syndrome; D014960:X Chromosome