Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenemia assay.
Quantitative cytomegalovirus antigenemia and DNAemia were determined in peripheral leukocytes of 25 patients stored for up to 72 h at room temperature (RT) and 4 degrees C before processing. Numbers of antigen-positive cells significantly decreased with time. The decline was greater at RT than at 4 degrees C. In contrast, no significant alterations in DNAemia occurred.
Schäfer
P
P
Institut für Medizinische Mikrobiologie und Immunologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany.
Tenschert
W
W
Gutensohn
K
K
Laufs
R
R
D000956:Antigens, Viral / Q000097:blood*; D001800:Blood Specimen Collection; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005914:Globins / Q000235:genetics; D006801:Humans; D007962:Leukocytes / Q000821:virology; D010750:Phosphoproteins / Q000097:blood / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014763:Viral Matrix Proteins / Q000097:blood / Q000276:immunology; D014773:Virology / Q000379:methods
D000956:Antigens, Viral; D004279:DNA, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D005914:Globins