Pharmacokinetic studies on 5-aminolevulinic acid-induced protoporphyrin IX accumulation in tumours and normal tissues.
Laser-induced fluorescence (LIF) for in vivo point monitoring and fluorescence microscopy incorporating a CCD camera were used to study the fluorescence distribution of 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) in tumours. Fluorescence in a chemically-induced adenocarcinoma in the liver of rats and in an aggressive basal cell carcinoma in a patient were studied after intravenous injection of ALA at a dose of 30 mg/kg body weight. The LIF technique demonstrated slightly more ALA-induced PpIX fluorescence in the tumour than in the surrounding normal liver and abdominal muscle of rats. The visible parts of the human basal cell carcinoma exhibited strong ALA-induced fluorescence, while this fluorescence was much weaker in the necrotic areas of the tumour and in the surrounding normal skin.
Heyerdahl
H
H
Department of Surgical Oncology, Norwegian Radium Hospital, Montebello, Oslo, Norway.
Wang
I
I
Liu
D L
DL
Berg
R
R
Andersson-Engels
S
S
Peng
Q
Q
Moan
J
J
Svanberg
S
S
Svanberg
K
K
D000230:Adenocarcinoma / Q000139:chemically induced / Q000378:metabolism*; D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000818:Animals; D002280:Basal Cell Carcinoma / Q000139:chemically induced / Q000378:metabolism*; D004347:Drug Interactions; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000378:metabolism* / Q000494:pharmacology*; D011524:Protoporphyrins / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D011920:Rats, Inbred WF; D012867:Skin / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX