Transposon mutagenesis of Haemophilus paragallinarum with Tn916.
The Gram-positive transposon Tn916 was introduced into two strains of Haemophilus paragallinarum by electroporation of the suicide plasmid pAM120 (pGL101::Tn916). Tetracycline resistant mutants of H. paragallinarum strains Tw-1 and 221 were recovered at frequencies of 2.1 x 10(-6) and 4.5 x 10(-7) per viable cell electroporated, respectively. Tn916 generates stable single insertions within different sites of the H. paragallinarum chromosome. One Tw-1::Tn916 mutant had the Tn916 insertion in the cryptic plasmid p250. This study indicates the potential use of Tn916 as a genetic tool for insertional mutagenesis of H. paragallinarum.
Gonzales
A E
AE
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602-487, USA.
Glisson
J R
JR
Jackwood
M W
MW
D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D018274:Electroporation; D006094:Gram-Positive Bacteria / Q000235:genetics; D006190:Haemophilus / Q000235:genetics*; D016254:Mutagenesis, Insertional; D010957:Plasmids; D015183:Restriction Mapping; D013753:Tetracycline Resistance / Q000235:genetics
D004251:DNA Transposable Elements; D004269:DNA, Bacterial