Stimulation of insulin release by alpha-D-glucose pentaacetate.
Insulin release from rat pancreatic islets was stimulated by alpha-D-glucose pentaacetate (1.7 mM), but not by an equimolar concentration of beta-D-galactose pentaacetate. The secretory response to alpha-D-glucose pentaacetate was not reproduced by D-glucose and/or acetate, tested at concentrations equimolar to that of the hexose ester, and failed to be adversely affected by 3-O-methyl-D-glucose, even when used at a concentration sufficient to inhibit glucose-stimulated insulin release. These findings suggest that the insulinotropic action of alpha-D-glucose pentaacetate is attributable, in part at least, to the intracellular generation of D-glucose from the ester. The present work thus introduces selected esters of D-glucose as tools for the cellular supply of the hexose by a process that does apparently not involve its carrier-mediated transport across the plasma membrane.
Malaisse
W J
WJ
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Jijakli
H
H
Kadiata
M M
MM
Sener
A
A
Kirk
O
O
D019325:3-O-Methylglucose / Q000494:pharmacology; D000818:Animals; D004347:Drug Interactions; D005260:Female; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D005973:Glutamine / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D007651:Keto Acids / Q000494:pharmacology; D007930:Leucine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D007651:Keto Acids; D005973:Glutamine; D019325:3-O-Methylglucose; C105128:glucose pentaacetate; C013082:alpha-ketoisocaproic acid; D007930:Leucine; D005947:Glucose