The effect of Iloprost (ZK 36374) on creatine phosphate, adenine nucleotides and superoxide dismutase activity in ischaemic canine myocardium.
Ischaemic injury was produced in the dog heart by occluding the left anterior descending coronary artery just below the second diagonal branch for the duration of 3 h. A prostacyclin analogue, ZK 36374 (Iloprost) was administered (10 micrograms/kg) just before the coronary artery occlusion. During 3 h occlusion ATP levels in the ischaemic area declined from 65.4 +/- 7.1 mM/g protein in sham-operated group to 31.5 +/- 5.2 microM/g protein in no-drug group and to 44.1 +/- 5.0 microM/g protein in Iloprost group. Creatine phosphate decreased from 112 +/- 14 microM/g protein, to 81 +/- 10 microM/g protein in no-drug group and to 95 +/- 12 microM/g protein in Iloprost group. The energy charge (ATP + 0.5 ADP/ATP + ADP + AMP) decreased slightly but not significantly in no-drug and Iloprost group. Three hours of LAD occlusion produced a significant fall in SOD activity in the ischaemic heart in comparison to the non-ischaemic and Iloprost-treated hearts.
Ogonowska-Kobusiewicz
M
M
Department of Anaesthesiology, Medical Academy, Agricultural University, Lublin, Poland.
Kobusiewicz
W
W
Michalak
J
J
Ledwozyw
A
A
D000227:Adenine Nucleotides / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D016285:Iloprost / Q000494:pharmacology*; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D009206:Myocardium / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D010725:Phosphocreatine / Q000032:analysis* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology*
D000227:Adenine Nucleotides; D014665:Vasodilator Agents; D010725:Phosphocreatine; D013482:Superoxide Dismutase; D016285:Iloprost