The augmenting effect of OK432-stimulated B cells on the in vitro generation of anti-tumor cytotoxic T lymphocytes from tumor-draining lymph node cells: the possible role of interleukin-12.
Effect of a local injection with a streptococcal preparation OK432 on the in vitro generation of anti-tumor cytotoxic T lymphocytes (CTLs) from tumor-draining lymph nodes (LN) was investigated. A peritumoral injection with OK432 on days 2, 4, 6 and 8 significantly increased both the total cell number and the proportion of B cells in the draining LN cells on day 10 after a subcutaneous inoculation with B16 melanoma. In an in vitro proliferative assay, OK432 showed a stimulatory effect on both normal splenic T and B cells. In a cytolytic assay, the OK432-injected B16-draining LN cells showed a higher level of anti-B16 CTL activity than the B16-draining LN cells after in vitro restimulation. This augmenting effect of OK432 was dependent on the B cells. Moreover, nonadherent cells from the OK432-injected B16-draining LN cells showed a low but significantly higher level of anti-B16 CTL activity than those from the B 16-draining LN cells after in vitro restimulation, whereas this augmenting effect of OK432 was abolished by the in vitro addition of anti-interleukin (IL)-12 monoclonal antibody. Collectively, these findings suggest that the augmenting effect of a local injection with OK432 on the potential of tumor-draining LN cells to turn into anti-tumor CTLs after in vitro restimulation was at least in part due to IL-12 derived from the OK432-stimulated B cells.
Shinomiya
Y
Y
Department of Immunology, Medical Institute of Bioregulation Kyushu University, Fukuoka, Japan.
Harada
M
M
Tamada
K
K
Kurosawa
S
S
Okamoto
T
T
Terao
H
H
Takenoyama
M
M
Ito
O
O
Hirashima
C
C
Li
T
T
Shirakusa
T
T
Nomoto
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000382:microbiology; D001667:Binding, Competitive / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D005260:Female; D018664:Interleukin-12 / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D016399:Lymphoma, T-Cell; D008545:Melanoma; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D013291:Streptococcus; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000276:immunology / Q000382:microbiology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000382:microbiology
D000911:Antibodies, Monoclonal; D018664:Interleukin-12