Cloning of a novel member of the G protein-coupled receptor family related to peptide receptors.
We have used PCR with degenerate oligonucleotide primers to clone novel members of the G protein-coupled receptor (GPCR) superfamily. We report here a novel gene, CEPR, which encodes a candidate receptor that is most similar to the peptide receptor family. The coding region of the human CEPR gene predicts a seven transmembrane domain (TM) receptor of 375 amino acids. CEPR has 28-30 percent amino acid identity to angiotensin II and interleukin 8 receptors, and slightly lower percent identity to many other GPCRs. Northern blot analysis reveals a 3.3 kb CEPR transcript in different regions of human brain and in various peripheral tissues. The ubiquitous tissue distribution of CEPR, its expression in early development, and its conservation in evolution indicate a potentially important biological function for this receptor and its putative peptide ligand.
Feng
Y
Y
Institute for Metabolic Disorders, Bayer Corporation, West Haven, Connecticut 06516, USA.
Gregor
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D019204:GTP-Binding Proteins / Q000502:physiology; D015870:Gene Expression; D005796:Genes; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D018000:Receptors, Peptide / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014018:Tissue Distribution
D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018000:Receptors, Peptide; D019204:GTP-Binding Proteins