Methods and reagents. Reducing background colonies with positive selection vectors.
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses the pros and cons of eliminating unwanted background colonies by using the positive selection vector pZErO. For details on how to partake in the newsgroup, see the accompanying box.
Hengen
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PN
National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA. pnh@ncifcrf.gov
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D001761:Bleomycin / Q000494:pharmacology; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D011933:Reagent Kits, Diagnostic / Q000191:economics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D012641:Selection, Genetic; D001616:beta-Galactosidase / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C068356:CcdB protein, Plasmid F; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D001761:Bleomycin; C105427:Zeocin; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase