title: Cell adhesion molecule expression within human glomerular and kidney organ culture.
abstract: A glomerular and kidney organ culture method was developed to study cytokine inducibility of adhesion molecules and MHC antigen expression. Glomerular cells showed constitutive expression of intercellular cell adhesion molecule-1 (ICAM-1) and MHC class I and II antigens, but not vascular cell adhesion molecule-1 (VCAM-1), E-selectin, or P-selectin. Expression of E-selectin on glomerular endothelial cells (ECs) was induced by interleukin-1 (IL-1), tumour necrosis factor (TNF), interferon-gamma (IFN-gamma) and granulocyte/ macrophage-colony stimulating factor (GM-CSF); this induction was inhibited by transforming growth factor beta (TGF beta) and by IL-4. P-selectin expression was never seen within glomeruli. VCAM-1 was constitutively expressed by Bowman's capsule and proximal tubules and was weakly induced on glomerular ECs by TNF and IL-4 in combination. Glomerular endothelial ICAM-1 expression was increased by IL-1, TNF, IFN-gamma, and GM-CSF, while TGF beta inhibited induction by TNF and IL-1. Expression of MHC class I and II antigens by glomerular ECs was constitutive; further upregulation of MHC class II by IFN-gamma was observed. These studies suggest that leukocyte-endothelial cell interactions that occur within the kidney follow broadly similar principles as are proposed to occur elsewhere in the body but, in addition, there are subtle differences that reflect local conditions.
authors.affiliation: Department of Medicine (CCRIS), Medical School, University of Birmingham, U.K.
authors.forename: C O
authors.initials: CO
authors.lastname: Savage
authors.forename: C J
authors.initials: CJ
authors.lastname: Brooks
authors.forename: D
authors.initials: D
authors.lastname: Adu
authors.forename: G
authors.initials: G
authors.lastname: Richards
authors.forename: A J
authors.initials: AJ
authors.lastname: Howie
mesh_terms: D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016207:Cytokines / Q000276:immunology; D006680:HLA Antigens / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007668:Kidney / Q000276:immunology*; D007678:Kidney Glomerulus / Q000276:immunology; D009924:Organ Culture Techniques; D019087:Selectins / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D006680:HLA Antigens; D019087:Selectins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1