A large open reading frame (orf1995) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein.
An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival.
Boudreau
E
E
Département de Biochimie, Faculté des sciences et de génie, Université Laval, Québec, Canada.
Turmel
M
M
Goldschmidt-Clermont
M
M
Rochaix
J D
JD
Sivan
S
S
Michaels
A
A
Leu
S
S
D020418:Algal Proteins; D000595:Amino Acid Sequence; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000254:growth & development; D002736:Chloroplasts / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid
D020418:Algal Proteins; D008565:Membrane Proteins