Identification of region specific genes by chromosome microdissection.
Chromosome microdissection is an extremely useful molecular cytogenetic tool for the characterization of chromosomal abnormalities in tumor cells. Although it has been used primarily in conjunction with fluorescence in situ hybridization (FISH), microdissection has also been useful for molecular analysis via microclone library construction. Recently, microdissection has been applied to the isolation of region specific cDNAs with sufficient success that gene discovery can now be added to the list of applications of this versatile molecular cytogenetic technique.
Meltzer
P S
PS
Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda, Maryland 20892-4470, USA.
Guan
X Y
XY
Su
Y A
YA
Gracia
E
E
Trent
J M
JM
D002874:Chromosome Mapping / Q000379:methods*; D018076:DNA, Complementary / Q000302:isolation & purification; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods
D018076:DNA, Complementary