Polyphasic taxonomic study of clinically significant actinomadurae including the description of Actinomadura latina sp.nov.
Thirty-one strains received either as Actinomadura madurae or Actinomadura pelletieri were assigned to four phena, clusters 1 to 4, in a numerical phenetic survey of the genus Actinomadura. Clusters 2 and 4 corresponded to the validly described species A. madurae and A. pelletieri whereas clusters 1 and 3 encompassed strains received as A. madurae and A. pelletieri, respectively. The two clusters that contained A. madurae strains formed a single taxon when a dataset lacking the antibiotic sensitivity entries was examined. Results from pyrolysis mass spectrometric and DNA amplification fingerprinting analyses underpinned the taxonomic status of clusters 2, 3 and 4 and suggested that the A. madurae strains accounted for a relatively wide range of variation. It is proposed that the cluster 3 strains be given species status within the genus Actinomadura given the congruence found between the chemical, molecular and numerical phenetic data. The name Actinomadura latina is proposed for the new taxon. The type strain is DSM 43382.
Trujillo
M E
ME
Department of Microbiology, Medical School, Newcastle-upon-Tyne, UK.
Goodfellow
M
M
D000192:Actinomycetales / Q000145:classification*; D004269:DNA, Bacterial / Q000032:analysis; D005784:Gene Amplification; D006801:Humans; D013058:Mass Spectrometry
D004269:DNA, Bacterial