title: Examination of virus shedding in semen from vaccinated and from previously infected boars after experimental challenge with porcine reproductive and respiratory syndrome virus.
abstract: Danish artificial insemination (AI) centres house several boars antibody positive to porcine reproductive and respiratory syndrome virus as well as PRRSV-naive boars which may become acutely infected. The risk of transmission of PRRSV by semen may therefore constitute a serious problem to the Danish pig industry. The use of a vaccination-program may be a way to avoid or reduce the problem. This study evaluates the use of two vaccines: One live, attenuated vaccine and one inactivated vaccine. A pronounced reduction in viremia and shedding of virus in semen was demonstrated by use of the live vaccine compared to the non-vaccinated control animals. In contrast, no changes in onset, level and duration of viremia and shedding of virus in semen were observed using the inactivated vaccine. Neither viremia nor seminal shedding of virus was detected in previously PRRSV-infected, PRRSV-antibody positive boars after challenge with a Danish field strain of PRRSV.
authors.affiliation: Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark.
authors.forename: T L
authors.initials: TL
authors.lastname: Nielsen
authors.forename: J
authors.initials: J
authors.lastname: Nielsen
authors.forename: P
authors.initials: P
authors.lastname: Have
authors.forename: P
authors.initials: P
authors.lastname: Baekbo
authors.forename: R
authors.initials: R
authors.lastname: Hoff-Jørgensen
authors.forename: A
authors.initials: A
authors.lastname: Bøtner
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001831:Body Temperature; D003718:Denmark; D004797:Enzyme-Linked Immunosorbent Assay; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control / Q000635:transmission*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology / Q000302:isolation & purification*; D012661:Semen / Q000821:virology*; D013552:Swine; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines