title: Serodiagnostic value of ELISA in pulmonary tuberculosis in Turkey where tuberculosis is highly prevalent.
abstract: We studied the diagnostic utility of ELISA in pulmonary tuberculosis (TBC) using serologically active glycolipid antigens. Twenty-seven patients who were smear positive, and 30 patients who were smear negative, but with evidence of active TBC (sputum culture positive in 10, response to anti-TBC chemotherapy in 20) were included in the study group. Twenty cured patients who had been free of TBC for at least 1 year, 50 TBC-free persons with PPD results of 0-10 mm and more than 10 mm, and 21 patients with active inflammatory diseases other than TBC formed the control groups. Sensitivity and specificity were 96 and 91%, respectively. We think that serologically active glycolipid antigens are as sensitive and specific as other purified antigens, and even superior to them from the point of view of their production: their extraction is quicker and easier.
authors.affiliation: Department of Medicine, Faculty of Medicine, Cukurova University, Adana, Turkey.
authors.forename: U B
authors.initials: UB
authors.lastname: Doğan
authors.forename: H S
authors.initials: HS
authors.lastname: Aksu
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000302:isolation & purification; D000995:Antitubercular Agents / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006017:Glycolipids / Q000302:isolation & purification; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000276:immunology*; D015995:Prevalence; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology; D014421:Turkey / Q000453:epidemiology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000995:Antitubercular Agents; D006017:Glycolipids