title: [PCR-detection of cytomegalovirus infection in oncologic and non-oncologic patients with organ and tissue transplantation].
abstract: Cytomegalovirus (CMV) infection often causes death after organ transplantation. Therefore, adequate prevention, diagnosis and treatment of this complication are of critical importance. The experience of establishing routine PCR-detection of CMV is presented. A widely accepted PCR protocol was used for clinical purposes. The strategy included 3 steps: 1) PCR amplification of CMV-sequences from leukocytes of patients; 2) visualization of PCR-product in polyacrylamide gel; 3) verification of the identity of PCR product by its hybridization to a membrane-bound specific probe. This approach allowed to detect CMV infection in 10 out of 28 bone marrow and in 4 out of 32 kidney recipients. The administration of specific antiviral treatment led to the elimination of CMV sequences from the blood.
authors.forename: I N
authors.initials: IN
authors.lastname: Aleksandrova
authors.forename: I V
authors.initials: IV
authors.lastname: Koshkarova
authors.forename: A A
authors.initials: AA
authors.lastname: Lyshchev
authors.forename: K O
authors.initials: KO
authors.lastname: Sitskaia
authors.forename: L S
authors.initials: LS
authors.lastname: Zubarovskaia
authors.forename: S L
authors.initials: SL
authors.lastname: Akimova
authors.forename: L N
authors.initials: LN
authors.lastname: Shabanova
authors.forename: G F
authors.initials: GF
authors.lastname: Pluzhnikova
authors.forename: V I
authors.initials: VI
authors.lastname: Rudenko
authors.forename: K P
authors.initials: KP
authors.lastname: Khanson
authors.forename: B V
authors.initials: BV
authors.lastname: Afanas'ev
mesh_terms: D000998:Antiviral Agents / Q000627:therapeutic use; D016026:Bone Marrow Transplantation; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification*; D006801:Humans; D016030:Kidney Transplantation; D014184:Transplantation, Homologous
chemical_list: D000998:Antiviral Agents; D015342:DNA Probes; D004279:DNA, Viral
vernacular_title: PTSR-diagnostika tsitomegalovirusnoĭ infektsii u onkologicheskikh i neonkologicheskikh bol'nykh, perenesshikh transplantatsiiu organov i tkaneĭ.