Monoamine oxidase activity in the uterine and mesenteric arteries, vessels of ovarian pedicle and myometrium of pigs during the oestrous cycle.
Activities of total monoamine oxidase (MAO-T) and of both subtypes: MAO-A and MAO-B were examined in the uterine artery, vessels of ovarian pedicle (subovarian veno-arterial network), mesenteric artery and myometrium on the 0-2nd, 13-14th and 16-18th days of the oestrous cycle of pigs. It was found that in the uterine artery MAO-T, MAO-A and MAO-B activities were the highest on the 0-2nd days (100%); on day 13-14 they were lower by 85.4, 84.8 and 87.4% (p < 0.01), respectively, and on days 16-18 they were lower by 52.4, 57.5 and by 54.1% (p < 0.01) in comparison with the value on the 0-2nd days of ovarian cycle. The activities of MAO-T, MAO-A and MAO-B in the vessels of ovarian pedicle on the 0-2nd days were the lowest, whereas on days 13-14 they were the highest (p < 0.01), MAO-T by 445.3%, MAO-A by 315.6%, MAO-B by 344.7%, and they were higher on days 16-18 by 133.8% (p < 0.01), 85.9 (p < 0.05) and by 281.5% (p < 0.01) in comparison with the value on the 0-2nd day of ovarian cycle, respectively. The activities of examined enzymes in mesenteric artery were higher on 0-2nd and 16-18th days, whereas MAO-T and MAO-B they were lower by 39.0% and MAO-A by 22.3% (p < 0.05) on 13-14th days of oestrous cycle. In myometrium the activities of MAO-T, MAO-A and MAO-B on the 0-2nd days (100%) were the lowest, whereas on days 13-14 the activities were the highest (p < 0.01), MAO-T by 326.3%, MAO-A by 363.3% and MAO-B by 364.1%, and on days 16-18 the MAO-T and MAO-B activities were also higher by 99.4% and 150.7% (p < 0.01) respectively, whereas MAO-A activity was similar to the value on the 0-2nd days. The high MAO activity in the vessels of pig ovarian pedicle may be a significant factor in the ovarian vasotone decrease and increase in the ovarian blood flow during the luteal phase of ovarian cycle.
Dynarowicz
I
I
Department of Pharmacology, Faculty of Veterinary Medicine, Olsztyn University of Agriculture and Technology, Poland.
Szurmiński
M
M
D000704:Analysis of Variance; D000818:Animals; D001158:Arteries / Q000201:enzymology; D004971:Estrus / Q000378:metabolism / Q000502:physiology*; D005260:Female; D008638:Mesenteric Arteries / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000032:analysis* / Q000502:physiology; D009215:Myometrium / Q000098:blood supply*; D010053:Ovary / Q000098:blood supply*; D012039:Regional Blood Flow / Q000502:physiology; D013050:Spectrometry, Fluorescence / Q000662:veterinary; D013552:Swine / Q000378:metabolism / Q000502:physiology*; D014599:Uterus / Q000098:blood supply*
D008995:Monoamine Oxidase