title: A large open reading frame (orf1995) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein.
abstract: An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival.
authors.affiliation: Département de Biochimie, Faculté des sciences et de génie, Université Laval, Québec, Canada.
authors.forename: E
authors.initials: E
authors.lastname: Boudreau
authors.forename: M
authors.initials: M
authors.lastname: Turmel
authors.forename: M
authors.initials: M
authors.lastname: Goldschmidt-Clermont
authors.forename: J D
authors.initials: JD
authors.lastname: Rochaix
authors.forename: S
authors.initials: S
authors.lastname: Sivan
authors.forename: A
authors.initials: A
authors.lastname: Michaels
authors.forename: S
authors.initials: S
authors.lastname: Leu
mesh_terms: D020418:Algal Proteins; D000595:Amino Acid Sequence; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000254:growth & development; D002736:Chloroplasts / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid
chemical_list: D020418:Algal Proteins; D008565:Membrane Proteins