title: Detached retina affects morphologic and biochemical changes in the retina adjacent to bullous retinal detachment in rabbits.
abstract: OBJECTIVE
  Long-term results, more than 10 years after successful retinal detachment surgery, have shown gradually decreasing visual acuity in some cases. It is unclear if reduced functional recovery postoperatively is caused by anatomic changes or biochemical disorders. To determine the etiology of the reduced visual acuity, we cytochemically examined the changes in the cellular responses of the edges of retinal detachments.
  
  
  METHODS
  We histochemically studied the glucose-6-phosphatase (G6P) and 5'-nucleotidase (5'-Nase) activity in the rabbit retina. Experimental rhegmatogenous retinal detachment was produced in a rabbit model after partial vitrectomy, followed by retinal tear formation.
  
  
  RESULTS
  Although 5'-Nase activity gradually decreased during the period of detachment, activity was still detectable after 24 weeks. G6P activity increased in the region of the detached neural retina. Around the border of the detached retina, the decrease in 5'-Nase activity extended approximately 140 micrometers into the adjacent attached retina at 2 weeks after detachment and 270 micrometers at 24 weeks.
  
  
  CONCLUSIONS
  These observations suggest that some anatomical and biochemical damages may occur in the retina adjacent to bullous retinal detachment and may explain the reduction in postoperative vision in some clinical cases.
authors.affiliation: Department of Ophthalmology, Kyoto University, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Honda
authors.forename: A
authors.initials: A
authors.lastname: Ohira
authors.forename: H
authors.initials: H
authors.lastname: Ishigooka
authors.forename: S
authors.initials: S
authors.lastname: Ueno
authors.forename: Y
authors.initials: Y
authors.lastname: Honda
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D005260:Female; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D006651:Histocytochemistry; D008297:Male; D011817:Rabbits; D012160:Retina / Q000201:enzymology / Q000648:ultrastructure; D012163:Retinal Detachment / Q000201:enzymology* / Q000209:etiology / Q000473:pathology*; D012167:Retinal Perforations / Q000150:complications; D014821:Vitrectomy / Q000009:adverse effects
chemical_list: D015720:5'-Nucleotidase; D005952:Glucose-6-Phosphatase