Sequence-specific cleavage of yeast tRNA(Phe) with oligonucleotides conjugated to a diimidazole construct.
Oligonucleotide derivatives conjugated to a chemical construction with two histamine residues imitating the catalytic center of ribonuclease A have been synthesized. In experiments with the conjugates complementary to the 3'-end and to the variable loop and the T loop of yeast tRNA(Phe), it was shown that the compounds can accomplish sequence-specific cleavage of the target RNA in physiologic conditions.
Vlassov
V
V
Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Abramova
T
T
Godovikova
T
T
Giege
R
R
Silnikov
V
V
D001483:Base Sequence; D007093:Imidazoles / Q000737:chemistry*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D012331:RNA, Fungal / Q000187:drug effects*; D012360:RNA, Transfer, Phe / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology; D015003:Yeasts / Q000235:genetics
D007093:Imidazoles; D016376:Oligonucleotides, Antisense; D012331:RNA, Fungal; D012360:RNA, Transfer, Phe; D012259:Ribonuclease, Pancreatic