Electrospray mass spectrometric characterization of a corticosteroid dimer.
18-Hydroxy-11-deoxycorticosterone forms a non-polar dimer under acidic conditions. The dimer was purified by high performance liquid chromatography and further analysed by electrospray mass spectrometry. The purified dimer has a molecular weight of 656 Da with two free ketone groups and no free hydroxyl functions. The mass spectrometric data suggest a 20,21,20',21'-anhydro dimer structure for the dimer. Microbore high performance liquid chromatography/electrospray mass spectrometry of rat adrenal extracts indicated that both 18-OH-B and 18-OH-DOC were present, although no 18-OH-DOC dimer was detected.
Rendell
N B
NB
Department of Clinical Pharmacology, Royal Postgraduate Medical School, London, UK.
Taylor
G W
GW
Vinson
G P
GP
D000305:Adrenal Cortex Hormones / Q000737:chemistry* / Q000302:isolation & purification; D000311:Adrenal Glands / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D013058:Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar
D000305:Adrenal Cortex Hormones