Frequent association of alternative splicing of NER, a nuclear hormone receptor gene in cancer tissues.
We have detected frequent alternative splicing of a gene that encodes NER, a protein homologous to the retinoic acid receptors, in cancer cells. Western and immunohistochemical analyses disclosed accumulation of a large amount of the aberrant NER product, generated by alternative splicing that caused skipping of an exon corresponding to the DNA-binding domain, in the nucleoli of cells of cancer cell lines and primary cancer tissues. The aberrant protein was detected in 116 of 228 primary cancers developed in various tissues including breast and colon, but was absent in the corresponding normal tissues; it was also detected in 31 of 39 cancer cell lines. This observation may imply that the aberrant NER product has some relation to the development and/or progression of cancers in a variety of human tissues.
Saito
H
H
Department of Human Genome Analysis, Cancer Institute, Toshima-ku, Tokyo, Japan.
Nakatsuru
S
S
Inazawa
J
J
Nishihira
T
T
Park
J G
JG
Nakamura
Y
Y
D017398:Alternative Splicing; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics; D003110:Colonic Neoplasms / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism; D017931:DNA Primers; D018450:Disease Progression; D004938:Esophageal Neoplasms / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D000071518:Liver X Receptors; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D057093:Orphan Nuclear Receptors; D010051:Ovarian Neoplasms / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics; D011987:Receptors, Steroid / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D017931:DNA Primers; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid