Development of aberrant crypt foci involves a fission mechanism as revealed by isolation of aberrant crypts.
Morphological analysis of isolated colonic crypts in rats, postnatally, indicated that the crypts reproduce themselves by a fission mechanism, the division beginning at the crypt base and proceeding upwards until there are two separate crypts. Occasionally, before the separation is complete, a second fission process starts on one or both sides of a bifurcating crypt and a triple-branched or quadruple-branched crypt results. Analysis of isolated aberrant crypt foci (ACF) in rats treated with 1,2-dimethylhydrazine revealed that the development of ACF consisting of multiple crypts is also due to a fission mechanism. Initially, an indentation appears at the base of a single ACF crypt, with subsequent formation of a bifurcation and eventual crypt division.
Fujimitsu
Y
Y
Laboratory of Pathology, Aichi Cancer Research Institute, Chikusa-ku, Nagoya.
Nakanishi
H
H
Inada
K
K
Yamachika
T
T
Ichinose
M
M
Fukami
H
H
Tatematsu
M
M
D019813:1,2-Dimethylhydrazine; D000818:Animals; D003106:Colon / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D004127:Dimethylhydrazines / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009153:Mutagens / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344
D004127:Dimethylhydrazines; D009153:Mutagens; D019813:1,2-Dimethylhydrazine