In vivo expression of a full-length cDNA copy of potato leafroll virus (PLRV) in protoplasts and transgenic plants.
A full-length cDNA copy (PLRVfl) of potato leafroll virus (PLRV) was constructed and examined in vivo for its biological activities by transient expression experiments with plasmid DNA or in vitro transcribed RNA. In addition, PLRVfl cDNA was stably introduced into the genome of potato plants by Agrobacterium-mediated leaf disc transformation. Both transient and stable expression of PLRVfl resulted in the synthesis of genomic and subgenomic PLRV RNAs. Transgenic plants accumulated the 17-kDa movement protein and displayed the typical symptoms of PLRV infection. This is the first example of the constitutive expression of a phloem-limited virus in planta.
Prüfer
D
D
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Schmitz
J
J
Tacke
E
E
Kull
B
B
Rohde
W
W
D016960:Agrobacterium tumefaciens; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004279:DNA, Viral / Q000235:genetics* / Q000502:physiology; D005822:Genetic Vectors; D017793:Luteovirus / Q000235:genetics* / Q000502:physiology; D010935:Plant Diseases / Q000821:virology; D010944:Plants; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000821:virology; D011198:Solanum tuberosum / Q000821:virology*; D014779:Virus Replication
D018076:DNA, Complementary; D004279:DNA, Viral