Identification of a novel antiapoptotic protein, GAM-1, encoded by the CELO adenovirus.
We have developed a simple screening method to identify genes that mimic bcl-2 or adenovirus E1B 19K in enhancing cell survival after transfection and have used this method to identify such a gene in the avian adenovirus CELO. The gene encodes a novel 30-kDa nuclear protein, which we have named GAM-1, that functions comparably to Bcl-2 and adenovirus E1B 19K in blocking apoptosis. However, GAM-1 has no sequence homology to Bcl-2, E1B 19K, or any other known antiapoptotic proteins and thus defines a novel antiapoptotic function.
Chiocca
S
S
Institute for Molecular Pathology, Vienna, Austria.
Baker
A
A
Cotten
M
M
D017501:Adenovirus E1B Proteins / Q000502:physiology; D000595:Amino Acid Sequence; D017209:Apoptosis; D000259:Aviadenovirus / Q000235:genetics*; D002467:Cell Nucleus / Q000821:virology; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology
D017501:Adenovirus E1B Proteins; C105052:CELO protein, adenovirus; D019253:Proto-Oncogene Proteins c-bcl-2; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins; D004253:DNA Nucleotidylexotransferase