Plastination of the larynx for whole-organ sectioning.
Whole-organ sectioning is an important technique for the assessment of laryngeal pathology. Since currently established methods require prior decalcification which causes morphological changes, the critical border area between cartilage and surrounding soft tissue cannot be investigated in the same specimen and morphometric studies are not possible. Plastination is a laboratory technique that has previously demonstrated its capacity to overcome these shortcomings. In so doing water and lipids are replaced by curable polymer within the laryngeal cells making decalcification unnecessary. In the present study, more than 50 human larynges were processed using block plastination (BP) and sheet plastination (SP). For BP the complete organ was plastinated as a whole and then cut into thin serial sections. For SP the fresh organ was sliced first and plastinated in a second step. Findings demonstrated that SP allowed for the production of whole-organ sections within a period of 1 week only. Section thicknesses were as thin as 15 mm using a diamond wire saw and an ultramilling device. Sectioning was possible in both coronary and horizontal planes. Following BP, specimens were cut in an industrial cutting machine to thicknesses of about 0.6 mm. Shrinkage of tissue was less than 10% for both methods. In all, SP was technically superior to routine paraffin histology, although cutting equipment is very expensive and delicate in handling. At present the technique of BP is the method of choice for macromorphometrical investigations on serial sections of the human larynx.
Sittel
C
C
Department of Otorhinolaryngology/Head and Neck Surgery, University of Cologne, Germany.
Eckel
H E
HE
Sprinzl
G M
GM
Stennert
E
E
D000096:Acetone; D001834:Body Water; D003365:Costs and Cost Analysis; D003648:Decalcification Technique; D017110:Freeze Substitution; D006801:Humans; D007817:Laryngeal Cartilages / Q000033:anatomy & histology; D007822:Laryngeal Neoplasms / Q000473:pathology; D007830:Larynx / Q000033:anatomy & histology*; D008055:Lipids; D008752:Methylene Chloride; D008867:Microtomy / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D016612:Paraffin Embedding; D016613:Plastic Embedding / Q000379:methods*; D011108:Polymers; D012117:Resins, Synthetic; D013499:Surface Properties; D014618:Vacuum
D008055:Lipids; D011108:Polymers; D012117:Resins, Synthetic; D000096:Acetone; D008752:Methylene Chloride