HLA-DRB1*04 high resolution typing.
High resolution typing for HLA-DR4 is required to identify the individual subtypes. In this study a panel of DR4-positive samples was typed by both sequencing-based typing (SBT) and oligohybridization (PCR sequence-specific oligonucleotide; PCR-SSO). SBT reveals the highest resolution; moreover, ambiguous DRB1*04 allelic combinations can be resolved by a selective amplification of the individual alleles and subsequent sequencing. An extended DR4-specific PCR-SSO makes high resolution typing possible; however, an additional protocol is required to resolve ambiguities.
Bouwens-Rombouts
A G
AG
Department of Pathology, University Hospital, Utrecht, The Netherlands.
Verduyn
W
W
Versluis
L F
LF
Schreuder
G M
GM
Tilanus
M G
MG
Giphart
M
M
D000483:Alleles; D004277:DNA, Single-Stranded; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D015804:HLA-DR4 Antigen / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction
D004277:DNA, Single-Stranded; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; D059811:HLA-DRB1 Chains; D009838:Oligodeoxyribonucleotides