The influence of surgical preparation on cyclic nucleotide synthesis in an organ culture of human saphenous vein.
OBJECTIVE
To investigate adenosine cyclic 3'5' monophosphate (cAMP) and guanosine cyclic 3'5' monophosphate (cGMP) synthesis in freshly isolated and surgically prepared human saphenous vein before and after culture.


METHODS
Bristol Heart Institute, Bristol, U.K.


METHODS
Freshly isolated and surgically prepared human saphenous vein was obtained from patients undergoing coronary artery bypass graft surgery. cAMP and cGMP synthesis, was assessed by radioimmunoassay in response to specific simulators in segments of saphenous veins after collection and following 14 days culture.


RESULTS
Immediately after collection there was a significant reduction in the synthesis of cAMP (forskolin and prostaglandin E1-stimulated) and cGMP (sodium nitroprusside-stimulated) in surgically prepared compared to freshly isolated saphenous veins. In contrast, following 14 days in culture, cAMP and cGMP synthesis was significantly elevated in surgically prepared compared to freshly isolated saphenous veins.


CONCLUSIONS
These data indicate that surgical preparation results in a marked reduction in cyclic nucleotide synthesis in saphenous vein which may be relevant to the pathophysiology of early vein graft failure. The normalisation of both cAMP and cGMP synthesis in surgically prepared veins following 14 days culture indicates that cyclic nucleotide synthesising capacity may not be a major determinant of neointima formation in this experimental model.
Jeremy
J Y
JY
University Department of Cardiac Surgery, Bristol Royal Infirmary, U.K.
Birkett
S D
SD
Bryan
A J
AJ
Angelini
G D
GD
D000109:Acetylcholine / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000527:Alprostadil / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D001026:Coronary Artery Bypass; D000242:Cyclic AMP / Q000096:biosynthesis*; D006152:Cyclic GMP / Q000096:biosynthesis*; D004247:DNA / Q000032:analysis; D006801:Humans; D008875:Middle Aged; D009924:Organ Culture Techniques; D011863:Radioimmunoassay; D012501:Saphenous Vein / Q000187:drug effects / Q000378:metabolism / Q000601:surgery*; D017539:Tunica Intima / Q000378:metabolism / Q000473:pathology
D005576:Colforsin; D000001:Calcimycin; D004247:DNA; D000242:Cyclic AMP; D000527:Alprostadil; D006152:Cyclic GMP; D000109:Acetylcholine