The barley Mlo gene: a novel control element of plant pathogen resistance.
Mutation-induced recessive alleles (mlo) of the barley Mlo locus confer a leaf lesion phenotype and broad spectrum resistance to the fungal pathogen, Erysiphe graminis f. sp. hordei. The gene has been isolated using a positional cloning approach. Analysis of 11 mutagen-induced mlo alleles revealed mutations leading in each case to alterations of the deduced Mlo wild-type amino acid sequence. Susceptible intragenic recombinants, isolated from mlo heteroallelic crosses, show restored Mlo wild-type sequences. The deduced 60 kDa protein is predicted to be membrane-anchored by at least six membrane-spanning helices. The findings are compatible with a dual negative control function of the Mlo protein in leaf cell death and in the onset of pathogen defense; absence of Mlo primes the responsiveness for the onset of multiple defense functions.
Büschges
R
R
The Sainsbury Laboratory, John Innes Centre, Norwich, United Kingdom.
Hollricher
K
K
Panstruga
R
R
Simons
G
G
Wolter
M
M
Frijters
A
A
van Daelen
R
R
van der Lee
T
T
Diergaarde
P
P
Groenendijk
J
J
Töpsch
S
S
Vos
P
P
Salamini
F
F
Schulze-Lefert
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D016923:Cell Death / Q000235:genetics; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000032:analysis; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D001467:Hordeum / Q000166:cytology / Q000235:genetics* / Q000382:microbiology; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D009174:Mycoplasma; D010940:Plant Proteins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D011994:Recombinant Proteins
D018744:DNA, Plant; D005819:Genetic Markers; C104922:MLO protein, Hordeum vulgare; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011994:Recombinant Proteins