title: An experimental study on effects of occlusion of the cystic duct in mice with or without emptying of the gallbladder.
abstract: To study consequences of cystic duct occlusion, 121 NMRI mice were operated: 40 had cystic duct ligature (L group), 41 had cystic duct ligature and evacuation of bile from the gallbladder (LE group), and 40 were sham-operated (S group). Ten mice from each group were sacrificed at 1 week, 1, 3 and 6 months, respectively. All but 2 mice showed signs of wellbeing during the observation time, and weight gain was the same in the three groups. Distended gallbladder occurred in 14 animals, 7 each in the L and the LE group, more frequent after 3 and 6 months. In the remainder mice the gallbladders were smaller than at the time of operation (p < 0.0001). Fibrosis and adhesions around the gallbladder were frequent in the L and the LE groups, and more pronounced in the latter (p = 0.0001). At microscopy there were signs of inflammation in the gallbladder wall in 16 cases in the L group, 28 in the LE group and 2 in the S group, and the degree of inflammation was more pronounced in the LE group than in the L group (p = 0.002). Eleven mice in the LE group had empyema, and slight intralumininal inflammation was more frequent in the LE group than in the L group (p < 0.0001). In conclusion, despite a high incidence of microscopic inflammation the study gave no indication that occlusion of the cystic duct did serious harm to the mice in this series.
authors.affiliation: Institute for Surgical Research, National Hospital, University of Oslo, Norway.
authors.forename: E
authors.initials: E
authors.lastname: Trondsen
authors.forename: A R
authors.initials: AR
authors.lastname: Rosseland
authors.forename: O P
authors.initials: OP
authors.lastname: Clausen
authors.forename: A
authors.initials: A
authors.lastname: Bakka
authors.forename: E
authors.initials: E
authors.lastname: Engh
authors.forename: E A
authors.initials: EA
authors.lastname: Rødland
authors.forename: A O
authors.initials: AO
authors.lastname: Aasen
mesh_terms: D000818:Animals; D002764:Cholecystitis / Q000209:etiology* / Q000473:pathology; D001651:Cholestasis, Extrahepatic / Q000473:pathology*; D003549:Cystic Duct; D005260:Female; D051379:Mice