Regulation of arachidonic acid release and prostaglandin formation by cell-cell adhesive interactions in wound repair.
Foetal calf serum (FCS) and platelet-derived growth factor (PDGF) induced arachidonate release in subconfluent murine 3T6 fibroblast cultures. However, the magnitude of this effect decreased significantly as the cell culture became confluent. Wound-induced injury of the fibroblast monolayer initiated a repair process that was potentiated by FCS or PDGF and which restored the integrity of cell monolayer. Under these experimental conditions, FCS and PDGF induced phospholipase A2 activity and subsequent arachidonic acid mobilization and production of eicosanoids in wounded fibroblast cultures. Finally, it is demonstrated that prostanoids, specifically prostaglandin E2, play an important role in cell proliferation induced by FCS and PDGF during wound repair.
Moreno
J J
JJ
Departamento de Ciencias Fisiológicas, Unidad de Fisiología, Facultad de Farmacia, Universidad de Barcelona, Av. Joan XXIII s/n, Barcelona E-08028, Spain.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*
D001095:Arachidonic Acids; D010982:Platelet-Derived Growth Factor; D011453:Prostaglandins