Deoxyribonuclease I-facilitated electrotransfer of protein-DNA complexes from electrophoretic gels to nitrocellulose membranes.
A simple and reproducible technique for efficient transfer of protein-DNA complexes from electrophoretic gels to nitrocellulose membranes is described. Transfer of DNA-protein complexes may be difficult, especially when the DNA is of high molecular mass. A considerable improvement in the efficiency of transfer can be achieved by directly digesting the DNA in the gel by DNase I. The method is illustrated in the example of the preferential binding of histone H1 to superhelical plasmid DNA.
Ivanchenko
M
M
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-7305, USA.
Zlatanova
J
J
D000818:Animals; D002645:Chickens; D003101:Collodion; D004278:DNA, Superhelical / Q000302:isolation & purification / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*
D004278:DNA, Superhelical; D006657:Histones; D003101:Collodion; D003850:Deoxyribonuclease I