title: Clinical reactivity to beef in children allergic to cow's milk.
abstract: BACKGROUND
  Cow's milk is one of the most common food allergens in children. Limited information is available on the prevalence of reactivity to a related food source, beef. The purposes of this study were to examine the prevalence of symptomatic sensitivity to beef in a selected pediatric population and to determine the frequency of concomitant reactivity to cow's milk and beef.
  
  
  METHODS
  Children referred for assessment of atopic dermatitis and possible food hypersensitivity were evaluated for symptomatic reactivity to beef by double-blind placebo-controlled food challenges (DBPCFCs) and subsequent open feedings of beef. Sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblot, and immunodot blot analyses were performed with patients' sera on preparations of beef extracts subjected to different cooking conditions: raw (no heating), medium, and well-cooked.
  
  
  RESULTS
  Eleven of 335 children referred for evaluation of atopic dermatitis and possible food hypersensitivity were found to have symptomatic sensitivity to beef; eight were also sensitive to milk, as demonstrated in previous DBPCFCs. Eight patients reacted to beef during DBPCFC, and three tolerated beef in a DBPCFC and well-cooked beef in an open challenge but reacted to ingestion of less well-cooked beef. SDS-PAGE of raw beef revealed at least 24 protein fractions. Several protein bands in raw beef appeared to denature with heating. Bovine serum albumin and bovine gamma globulin were heat-labile in the beef extract, but six protein fractions persisted even after heating the beef extract for 2 hours at 85 degrees C. IgE from patients reacting to rare and well-cooked beef bound up to six of these heat-resistant fractions, but IgE from patients reacting only to rare beef failed to bind any of these fractions with one exception. In addition, patients reacting to rare and well-cooked beef had specific IgE to a 17.8 kd fraction, which was only weakly recognized by one patient reacting only to rare beef.
  
  
  CONCLUSIONS
  Specific IgE antibodies to heat-labile beef proteins might explain why some patients can tolerate well-cooked beef but not medium-rare and rare beef. Patients reacting only to rare beef may not need to maintain a complete beef elimination diet.
authors.affiliation: Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Werfel
authors.forename: S K
authors.initials: SK
authors.lastname: Cooke
authors.forename: H A
authors.initials: HA
authors.lastname: Sampson
mesh_terms: D000293:Adolescent; D000818:Animals; D001249:Asthma / Q000150:complications; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000150:complications; D004044:Dietary Proteins / Q000032:analysis* / Q000276:immunology*; D004311:Double-Blind Method; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005511:Food Handling / Q000379:methods; D005512:Food Hypersensitivity / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D007223:Infant; D008460:Meat / Q000009:adverse effects*; D016269:Milk Hypersensitivity / Q000150:complications* / Q000276:immunology*; D012221:Rhinitis, Allergic, Perennial / Q000150:complications; D006255:Rhinitis, Allergic, Seasonal / Q000150:complications; D012710:Serum Albumin, Bovine / Q000032:analysis; D012882:Skin Tests; D014020:Tissue Extracts / Q000032:analysis / Q000276:immunology
chemical_list: D004044:Dietary Proteins; D007074:Immunoglobulin G; D014020:Tissue Extracts; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E