title: Expression of small, therapeutic RNAs in human cell nuclei.
abstract: Effective intracellular expression of small RNA therapeutics depends on a number of factors. The RNA, whether antisense, ribozyme, or RNA aptamer, must be efficiently transcribed, stabilized against rapid degradation, folded correctly, and directed to the part of the cell where it can be most effective. To overcome a number of these problems we have been testing expression cassettes based on the human tRNA(met) and U6 snRNA promoters, in which transcripts encoding small RNA inserts are protected against attack from the 3' and Transient expression in cultured cells results in 10(9)-2 x 10(7) full-length transcripts per cell, depending partially on the promoter construct used but also on the nature of the insert RNA 5' gamma-Phosphate methylation (capping) depended, as expected, on the inclusion of specific U6 snRNA sequences from positions +19 to +27. In situ localization of the transcripts shows that both tRNA and U6 promoter transcripts give primarily punctate nuclear patterns, and that capping of transcripts is not required for nuclear retention. Several different insert RNAs directed against HIV-1 were tested by cotransfection with HIV-1 provirus and assay for subsequent viral reverse transcriptase production. These include antisense RNA, hairpin and hammerhead ribozymes, and RNA ligands (aptamers) for Tat and Rev RNA binding proteins. Results show that Rev-binding RNAs efficiently block HIV-1 gene expression, whereas other RNAs have little or no effected when expressed in these cassettes.
authors.affiliation: Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0606, USA.
authors.forename: P D
authors.initials: PD
authors.lastname: Good
authors.forename: A J
authors.initials: AJ
authors.lastname: Krikos
authors.forename: S X
authors.initials: SX
authors.lastname: Li
authors.forename: E
authors.initials: E
authors.lastname: Bertrand
authors.forename: N S
authors.initials: NS
authors.lastname: Lee
authors.forename: L
authors.initials: L
authors.lastname: Giver
authors.forename: A
authors.initials: A
authors.lastname: Ellington
authors.forename: J A
authors.initials: JA
authors.lastname: Zaia
authors.forename: J J
authors.initials: JJ
authors.lastname: Rossi
authors.forename: D R
authors.initials: DR
authors.lastname: Engelke
mesh_terms: D001483:Base Sequence; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015658:HIV Infections / Q000628:therapy*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011533:Proviruses / Q000235:genetics*; D016337:RNA, Catalytic; D012342:RNA, Small Nuclear / Q000235:genetics*
chemical_list: D016337:RNA, Catalytic; D012342:RNA, Small Nuclear