title: In vivo and in vitro clomipramine treatment decreases the migration of macrophages in the rat.
abstract: We studied the effect of in vitro and in vivo treatment with the tricyclic antidepressant clomipramine on spontaneous mobility and on N-formyl-L-methionil-L-leucyl-L-phenylalanine (fMLP)-induced chemotaxis of rat peritoneal macrophages. When added in vitro clomipramine was able to diminish (from 10(-4) to 10(-7) M) both spontaneous and stimulated migration of macrophages. A similar effect was observed after the in vivo administration of the drug. In fact, both spontaneous and fMLP-induced mobility of peritoneal macrophages in vitro were significantly reduced after the subcutaneous injection of 20 and 40 mg/kg of clomipramine in comparison to the chemotaxis of macrophages obtained from saline-treated animals. These results give further evidence that psychoactive drugs can affect some immune parameters, and could contribute to explain the antiinflammatory action of clomipramine.
authors.affiliation: Department of Pharmacology, University of Milan, Italy. sacerdot@imiucca.csi.uniml.it
authors.forename: P
authors.initials: P
authors.lastname: Sacerdote
authors.forename: M
authors.initials: M
authors.lastname: Bianchi
authors.forename: A E
authors.initials: AE
authors.lastname: Panerai
mesh_terms: D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000008:administration & dosage / Q000494:pharmacology*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002997:Clomipramine / Q000008:administration & dosage / Q000494:pharmacology*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D007279:Injections, Subcutaneous; D008264:Macrophages / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000929:Antidepressive Agents, Tricyclic; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002997:Clomipramine