Expression of mRNA for the proto-oncogene c-fos in rat basophilic leukaemia cells.
Recently, the expression of the mRNA for the proto-oncogene c-fos following activation of the high-affinity receptor for immunoglobulin E in rodent mast cells has been reported. In the present study we investigated different biochemical events that may play a role in signal transduction pathways culminating in the expression of c-fos mRNA in rat basophilic leukaemia cells. Similar to IgE-mediated cell degranulation we demonstrated inhibition of the c-fos signal in the absence of calcium and after preincubation of cells with the protein tyrosine kinase inhibitor genistein. Activation of RBL-2H3 cells by short term PMA treatment failed to induce cell degranulation or expression of mRNA for c-fos. Depletion of protein kinase C by PMA pre-treatment resulted in substantial inhibition of the c-fos signal. In contrast to IgE-mediated cell degranulation, expression of mRNA for c-fos was not dependent on continued receptor aggregation. In addition, we demonstrate that c-fos mRNA expression is not restricted to Fc epsilon RI activation but can be induced by a variety of IgE independent mechanisms including calcium influx by ionophore A 23187 and stimulation of G proteins.
Stephan
V
V
Department of Pediatrics, Heinrich Heine University, Düsseldorf, Germany.
Seibt
A
A
Körholz
D
D
Wahn
V
V
D016475:3T3 Cells; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression; D019833:Genistein; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D015471:Leukemia, Basophilic, Acute; D051379:Mice; D011493:Protein Kinase C; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D012333:RNA, Messenger; D051381:Rats; D017455:Receptors, IgE / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D004791:Enzyme Inhibitors; D007529:Isoflavones; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D017455:Receptors, IgE; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D002118:Calcium