Expression cloning of GABA(B) receptors uncovers similarity to metabotropic glutamate receptors.
GABA (gamma-amino-butyric acid), the principal inhibitory neurotransmitter in the brain, signals through ionotropic (GABA(A)/ GABA(c)) and metabotropic (GABA(B)) receptor systems. Here we report the cloning of GABA(B) receptors. Photoaffinity labelling experiments suggest that the cloned receptors correspond to two highly conserved GABA(B) receptor forms present in the vertebrate nervous system. The cloned receptors negatively couple to adenylyl cyclase and show sequence similarity to the metabotropic receptors for the excitatory neurotransmitter L-glutamate.
Kaupmann
K
K
Novartis Pharma Inc., Research Department, Therapeutic Area Nervous System, Basel, Switzerland.
Huggel
K
K
Heid
J
J
Flor
P J
PJ
Bischoff
S
S
Mickel
S J
SJ
McMaster
G
G
Angst
C
C
Bittiger
H
H
Froestl
W
W
Bettler
B
B
D000262:Adenylyl Cyclases / Q000378:metabolism; D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D001386:Azides / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D019556:COS Cells; D002460:Cell Line; D002490:Central Nervous System / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli; D018756:GABA Antagonists / Q000494:pharmacology; D058786:GABA-B Receptor Agonists; D058788:GABA-B Receptor Antagonists; D017403:In Situ Hybridization; D008024:Ligands; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D018079:Receptors, GABA / Q000235:genetics* / Q000378:metabolism; D018080:Receptors, GABA-B / Q000235:genetics* / Q000378:metabolism; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D000345:Affinity Labels; D001386:Azides; D001565:Benzoates; C104981:CGP 64213; C104980:CGP 71872; D018076:DNA, Complementary; D018756:GABA Antagonists; D058786:GABA-B Receptor Agonists; D058788:GABA-B Receptor Antagonists; D008024:Ligands; D009943:Organophosphorus Compounds; D012333:RNA, Messenger; D018079:Receptors, GABA; D018080:Receptors, GABA-B; D018094:Receptors, Metabotropic Glutamate; D011994:Recombinant Proteins; D000262:Adenylyl Cyclases