title: Thrombopoietin is synergistic with other hematopoietic growth factors and physiologic platelet agonists for platelet activation in vitro.
abstract: Thrombopoietin (TPO) is the primary physiologic regulator of platelet production. The effect of TPO on platelet function, both alone and in combination with other hematopoietic growth factors, adenosine diphosphate (ADP), and epinephrine, was investigated using fluorescent-labeled antibodies to the activation-dependent antigen CD62 (P-selectin) and flow cytometry. TPO stimulated CD62 expression on normal human platelets, and this expression was completely inhibited by the soluble extracellular domain of the TPO receptor, MPL. The growth factors granulocyte colony-stimulating factor (G-CSF) and erythropoietin (EPO), but not interleukin-3 (IL-3) or stem-cell factor (SCF), also stimulated platelet activation. The combination of EPO, SCF, ADP, and epinephrine with TPO were synergistic for platelet CD62 expression. These data further support a role for TPO in modulating platelet function.
authors.affiliation: Department of Internal Medicine, University of California at Davis School of Medicine, California, USA.
authors.forename: T
authors.initials: T
authors.lastname: Wun
authors.forename: T
authors.initials: T
authors.lastname: Paglieroni
authors.forename: W P
authors.initials: WP
authors.lastname: Hammond
authors.forename: K
authors.initials: K
authors.lastname: Kaushansky
authors.forename: D C
authors.initials: DC
authors.lastname: Foster
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology*; D004357:Drug Synergism; D004837:Epinephrine / Q000494:pharmacology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006801:Humans; D015539:Platelet Activation / Q000187:drug effects*; D013926:Thrombopoietin / Q000494:pharmacology*
chemical_list: D016298:Hematopoietic Cell Growth Factors; D000244:Adenosine Diphosphate; D013926:Thrombopoietin; D004837:Epinephrine