Identifying changes in gene expression in the nervous system: mRNA differential display.
The majority of cellular and developmental processes are characterized by changes in gene expression. Recently, several polymerase chain reaction-based methods have been introduced for detecting genes whose expressions differ between cells or tissues. It is now possible to investigate whether novel gene expression occurs within many systems as a response to extracellular signals, and to identify systematically those genes. The characterization of cell-, tissue- or stage-specific gene expression will broaden our understanding of many complex biological processes.
Livesey
F J
FJ
MRC Laboratory of Molecular Biology, Cambridge, UK.
Hunt
S P
SP
D000818:Animals; D015870:Gene Expression; D006801:Humans; D009420:Nervous System / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*
D012333:RNA, Messenger