A novel transcription factor and two members of the Sp 1 multigene family regulate the activity of the alpha 2 (VI) collagen promoter.
For a better understanding of the processes that lead to the activation or inhibition of type VI collagen synthesis, we have identified and characterized the cis-acting elements of the chicken alpha 2 (VI) collagen promoter. This promoter encompasses four sites, termed S1, S2, X and S3, which interact with nuclear factors. Sites S1, S2 and S3 are each recognized by the same two proteins that belong to the Sp 1 multigene family. Site X appears to interact with a novel transcription factor of 43 kDa. When a fragment containing all four of the elements is placed in front of a reporter gene, the resulting construct is able to induce a high level of transcription in chicken fibroblasts. As soon as a single element is deleted from this construct, the activity decreases drastically. Thus, the four elements are essential for the transcriptional activation of the alpha 2 (VI) collagen gene.
Willimann
T E
TE
M. E. Müller-Institute for Biomechanics, University of Bern, Switzerland.
Maier
R
R
Trueb
B
B
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002642:Chick Embryo; D002645:Chickens; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics*; D018983:DNA Footprinting; D004742:Enhancer Elements, Genetic; D005347:Fibroblasts; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017384:Sequence Deletion; D016329:Sp1 Transcription Factor / Q000235:genetics*; D018808:Transcription Factor AP-1 / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D009841:Oligonucleotides; D016329:Sp1 Transcription Factor; D018808:Transcription Factor AP-1; D003094:Collagen