title: Expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in reactive and neoplastic lymphoid cells.
abstract: We have studied the expression of gelatinase A, gelatinase B, interstitial collagenase, tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 in reactive lymphoid cells, as well as in a series of cell lines derived from neoplasms of B- and T-cell lineage. Using both Northern blot analysis and zymography, gelatinase B activity was detected by zymography in two Burkitt cell lines and in a tonsillar cell suspension, while gelatinase A and interstitial collagenase activities were not detected by either method. TIMP-1 expression was demonstrated by Northern blot analysis in the multipotential neoplastic K-562 cell line, the high grade Burkitt's B-cell lymphoma lines, isolated tonsillar B cells and at low levels in peripheral blood T cells, but was not expressed in any of the neoplastic T-cell lines or isolated peripheral blood B cells. In contrast, TIMP-2 expression was restricted to tissues containing cells of T-cell lineage with high levels being observed in the neoplastic T-cell lines and lower levels in normal peripheral blood T cells and hyperplastic tonsil. Expression of TIMP-1 and TIMP-2 was confirmed at the protein level by reverse zymography and immunofluorescence assays using antihuman TIMP polyclonal antibodies. Expression of gelatinase B by the high grade B-cell Burkitt's lymphoma cell lines is consistent with previous findings in large cell immunoblastic lymphomas and indicates that this enzyme may play an important role in high grade non-Hodgkin's lymphomas. TIMP expression correlated with cell lineage in that TIMP-1 was primarily observed in B cells and TIMP-2 was restricted to T cells.
authors.affiliation: Flow Cytometry Unit, National Cancer Institute, Bethesda, NIH, Bethesda, MD 20892, USA.
authors.forename: M
authors.initials: M
authors.lastname: Stetler-Stevenson
authors.forename: A
authors.initials: A
authors.lastname: Mansoor
authors.forename: M
authors.initials: M
authors.lastname: Lim
authors.forename: P
authors.initials: P
authors.lastname: Fukushima
authors.forename: J
authors.initials: J
authors.lastname: Kehrl
authors.forename: G
authors.initials: G
authors.lastname: Marti
authors.forename: K
authors.initials: K
authors.lastname: Ptaszynski
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: W G
authors.initials: WG
authors.lastname: Stetler-Stevenson
mesh_terms: D001402:B-Lymphocytes / Q000201:enzymology*; D015152:Blotting, Northern; D017364:Collagenases / Q000096:biosynthesis*; D018093:Gelatinases / Q000096:biosynthesis*; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D008213:Lymphocyte Activation; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D013601:T-Lymphocytes / Q000201:enzymology*; D019716:Tissue Inhibitor of Metalloproteinase-2; D019714:Tissue Inhibitor of Metalloproteinases; D014407:Tumor Cells, Cultured
chemical_list: D006023:Glycoproteins; D019714:Tissue Inhibitor of Metalloproteinases; D019716:Tissue Inhibitor of Metalloproteinase-2; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9