1H and 13C NMR analysis of the pentasaccharide Gal beta (1-->4)GlcNAc beta (1-->3)-[GlcNAc beta (1-->6)]Gal beta (1-->4)GlcNAc synthesized by the mid-chain beta-(1-->6)-D-N-acetylglucosaminyltransferase of rat serum.
Chemical shifts and coupling constants of completely assigned 1H and 13C NMR spectra at 500 MHz, as well as ROESY and HMBC connectivities were used to establish the structure of the pentasaccharide Gal beta (1-->4)GlcNAc beta (1-->3)[GlcNAc beta (1-->6)]Gal beta (1-->4)GlcNAc, synthesized by the action of the mid-chain beta-(1-->6)-D-N-acetylglucosaminyltransferase of rat serum from UDP-GlcNAc and the linear tetrasaccharide Gal beta (1-->4)GlcNAc beta (1-->3)Gal beta (1-->4)GlcNAc.
Maaheimo
H
H
Institute of Biotechnology, University of Helsinki, Finland.
Räbinä
J
J
Renkonen
O
O
D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005690:Galactose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats
D009005:Monosaccharides; D009844:Oligosaccharides; C105300:galactopyranosyl-1-4-N-acetylglucosaminyl-1-3-(N-acetylglucosaminyl-1-6)galactopyranosyl-1-4-N-acetylglucosamine; D017351:N-Acetylglucosaminyltransferases; D000117:Acetylglucosamine; D005690:Galactose