Antibody specific for extra domain B of fibronectin demonstrates elevated levels of both extra domain B(+) and B(-) fibronectin in osteoarthritic canine cartilage.
A fusion protein containing the alternately spliced Extra Domain B [ED-B(+)] sequence of canine fibronectin was expressed in E. coli and the purified protein was used to produce an antibody specific for the ED-B(+) segment. This antibody recognized canine cartilage fibronectin but not canine plasma or synovial fluid fibronectin. Using this antibody, it was determined that ED-B(+) fibronectin increased proportionally with the total fibronectin in osteoarthritic cartilage. From this result, it was concluded that the chondrocyte is the source of much of the elevated fibronectin that appears in osteoarthritic cartilage.
Zang
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DW
James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.
Burton-Wurster
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N
Lust
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D000581:Amidohydrolases; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002356:Cartilage / Q000276:immunology / Q000378:metabolism*; D002358:Cartilage, Articular / Q000378:metabolism; D004285:Dogs; D005353:Fibronectins / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry; D010003:Osteoarthritis / Q000378:metabolism*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D013582:Synovial Fluid / Q000378:metabolism
D000906:Antibodies; D005353:Fibronectins; D011993:Recombinant Fusion Proteins; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase