Intracellular thiopurine nucleotides and azathioprine myelotoxicity in organ transplant patients.
OBJECTIVE
Despite widespread use of azathioprine in organ transplant recipients, the mechanism of its myelotoxicity remains unclear. The aim of this study was to assess the importance of thiopurine metabolites on bone marrow toxicity.


METHODS
We investigated the relationship between intracellular concentrations of 6-thioguanine (6-TGN), 6-mercaptopurine (6-MPN) and 6-thioxanthine (6 TXN) nucleotides and the absolute count of white or red cells in forty-seven lung or heart/lung transplant patients after oral administration of azathioprine.


RESULTS
No significant correlation between red cell concentrations of 6-TGN or total thiopurine metabolites and white or red cell counts was found, with no difference between the sexes. Likewise, high 6-TGN levels were not related to bone marrow depletion.


CONCLUSIONS
These results suggest that red blood cell 6-TGN alone do not predict the haematopoietic toxicity of azathioprine.
Boulieu
R
R
Université Claude bernard Lyon 1, Institut des Sciences Pharmaceutiques et Biologiques, Laboratoire de Pharmacie Clinique, France.
Lenoir
A
A
Bertocchi
M
M
Mornex
J F
JF
D000293:Adolescent; D000328:Adult; D000368:Aged; D001379:Azathioprine / Q000009:adverse effects*; D001853:Bone Marrow / Q000187:drug effects*; D005260:Female; D016041:Heart-Lung Transplantation; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D016040:Lung Transplantation; D008297:Male; D015122:Mercaptopurine / Q000097:blood*; D008875:Middle Aged; D013866:Thioguanine / Q000097:blood*; D014970:Xanthines / Q000097:blood*
D007166:Immunosuppressive Agents; D014970:Xanthines; C054700:6-thioxanthine; D015122:Mercaptopurine; D013866:Thioguanine; D001379:Azathioprine