Identification of fatty acid methyl esters (FAMEs) in postmortem tissue. A new marker of alcohol abuse?
Based on a method that combines thin layer chromatography and gas chromatography, it proved possible to detect postmortem fatty acid methyl esters (FAMEs) in human tissues (pancreas, liver, heart and adipose tissue). The highest concentrations were found in the pancreas, medium concentrations in the liver and adipose tissue and the lowest concentration in heart tissue. All tissues contained higher concentrations of unsaturated FAMEs than saturated FAMEs. In order to evaluate the influence of alcohol consumption on the formation of FAMEs, the decreased were divided into three groups: control group, chronic alcoholics (who were alcohol free at the time of death), and acutely alcohol-intoxicated subjects (so intoxicated at the time of death with no evidence of chronic alcohol abuse). In comparison to the control group, the organs of the chronic alcoholics showed only slight values, the tissues of the acutely intoxicated subjects were obviously higher. Based on the varying concentration distribution in the three groups, it seemed that postmortem differentiation of the alcohol consumption which existed before the time of death would be possible. After further investigation with a greater number of samples, FAMEs could become a useful supplement to existing alcohol markers in the future.
Emrich
J
J
Department of Alcohol Research, University of Göttingen, Germany.
Sprung
R
R
Sammler
J
J
Remberg
G
G
D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000437:Alcoholism / Q000175:diagnosis* / Q000378:metabolism; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005229:Fatty Acids, Monounsaturated / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D000432:Methanol / Q000097:blood; D008875:Middle Aged; D010179:Pancreas / Q000378:metabolism*; D011180:Postmortem Changes
D005229:Fatty Acids, Monounsaturated; D000432:Methanol