Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant.
A PCR method for the rapid identification and discrimination of thermophilic Campylobacter jejuni and Campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceuE). A nucleotide sequence divergence of approximately 13% in the ceuE genes of C. jejuni and C. coli facilitated the design of two species-specific PCR primer sets. The specificity of the PCR amplification reactions was confirmed by using two nonradioactively labelled species-specific internal oligonucleotide hybridization probes for each of these species.
Gonzalez
I
I
BBSRC Institute of Food Research, Reading Laboratory, Earley Gate, United Kingdom.
Grant
K A
KA
Richardson
P T
PT
Park
S F
SF
Collins
M D
MD
D001483:Base Sequence; D002169:Campylobacter Infections / Q000382:microbiology; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D006801:Humans; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D015345:Oligonucleotide Probes