Correlation of goblet cell density and mucosal epithelial membrane mucin expression with rose bengal staining in patients with ocular irritation.
OBJECTIVE
This study was designed to compare goblet cell densities and mucosal epithelial membrane mucin (MEM) expression in impression cytology specimens obtained from control subjects and patients with one of the following clinically defined diseases: aqueous tear deficiency (ATD) associated with Sjögren syndrome, ATD not associated with Sjögren syndrome, inflammatory Meibomian gland disease associated with rosacea, and Meibomian gland atrophy. These data were correlated with ocular surface rose Bengal staining scores, Schirmer scores, and HLA-DR antigen staining of conjunctival epithelial cells.


METHODS
Goblet cell density and MEM expression were studied by impression imprints with immunohistochemical staining using an anti-mucosal epithelial membrane mucin antibody in the temporal and inferior bulbar and inferior tarsal conjunctiva of study subjects.


RESULTS
Goblet cell density adjacent to the temporal limbus was significantly reduced at 3 mm posterior to the temporal limbus in both aqueous tear deficiency groups compared with the other groups and in patients with Sjögren syndrome compared with all other groups. In the inferior tarsus, goblet cell density was significantly reduced in patients with non-Sjögren syndrome ATD as compared with all other groups, except those with inflammatory Meibomian gland disease. Mucosal epithelial membrane mucin expression in the bulbar and tarsal conjunctiva was absent in a greater percentage of patients with Sjögren syndrome compared with all other groups. Total ocular surface rose Bengal staining scores were significantly higher in patients with Sjögren syndrome as compared with all other groups and in patients with non-Sjögren syndrome ATD as compared with control groups. Rose Bengal staining scores and Schirmer I test results (without anesthesia) were inversely correlated with bulbar, but not tarsal, conjunctival goblet cell densities, and with the absence of bulbar conjunctival MEM expression.


CONCLUSIONS
These results suggest that reduced goblet cell density and mucosal epithelial cell mucin expression could explain increased rose Bengal staining in patients with aqueous tear deficiency. In addition, MEM may be regarded as a marker for normal differentiation of ocular surface epithelia, with its absence signifying the development of squamous metaplasia.
Pflugfelder
S C
SC
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, FL 33136, USA.
Tseng
S C
SC
Yoshino
K
K
Monroy
D
D
Felix
C
C
Reis
B L
BL
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000378:metabolism; D015153:Blotting, Western; D002452:Cell Count; D003228:Conjunctiva / Q000378:metabolism* / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005128:Eye Diseases / Q000378:metabolism / Q000473:pathology; D005260:Female; D005456:Fluorescent Dyes; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D007124:Immunoenzyme Techniques; D007766:Lacrimal Apparatus Diseases / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008537:Meibomian Glands / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009077:Mucins / Q000096:biosynthesis*; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D012395:Rose Bengal; D012859:Sjogren's Syndrome / Q000378:metabolism* / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods; D013666:Tears / Q000378:metabolism
D005456:Fluorescent Dyes; D006684:HLA-DR Antigens; D009077:Mucins; D012395:Rose Bengal