title: Aerosolization of infectious virus by excimer laser.
abstract: OBJECTIVE
  To determine the potential for aerosolization of infectious virus present within the tear film during excimer laser photoablation of the cornea.
  
  
  METHODS
  Cell monolayers infected with herpes simplex virus or adenovirus, simulating virus-infected corneas, were ablated with the 193-nm excimer laser. Adjacent dishes containing noninfected cell monolayers were subsequently assayed for viral infection.
  
  
  RESULTS
  Viral spread to sentinel dishes occurred with both herpes simplex and adenovirus. The titer of virus present in the infected cell monolayers influenced the likelihood of spread to adjacent dishes. The presence of a vacuum aspiration system appeared to influence the direction of virus spread, with dishes located in the direction of the vacuum most likely to contain virus.
  
  
  CONCLUSIONS
  The potential for aerosolization of infectious virus exists with photoablation using a large-diameter excimer laser beam. Our experimental design, however, does not prove that spread of infectious virus is likely to occur in the clinical setting. Appropriate measures should be taken to reduce the possibility of the spread of virus from the patient to the surgeon, other medical staff, or other patients.
authors.affiliation: Doheny Eye Institute, Los Angeles, CA 90033, USA.
authors.forename: L B
authors.initials: LB
authors.lastname: Moreira
authors.forename: D
authors.initials: D
authors.lastname: Sanchez
authors.forename: M D
authors.initials: MD
authors.lastname: Trousdale
authors.forename: D
authors.initials: D
authors.lastname: Stevenson
authors.forename: F
authors.initials: F
authors.lastname: Yarber
authors.forename: P J
authors.initials: PJ
authors.lastname: McDonnell
mesh_terms: D000258:Adenovirus Infections, Human / Q000635:transmission*; D000260:Adenoviruses, Human / Q000502:physiology*; D000391:Air Microbiology; D000818:Animals; D002522:Chlorocebus aethiops; D006561:Herpes Simplex / Q000635:transmission*; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D054018:Lasers, Excimer; D008175:Lung Neoplasms / Q000821:virology; D018903:Photorefractive Keratectomy; D013552:Swine; D014407:Tumor Cells, Cultured; D014709:Vero Cells / Q000821:virology; D014776:Virus Cultivation