Cysteine-rich FGF receptor regulates intracellular FGF-1 and FGF-2 levels.
The cysteine-rich FGF receptor (CFR) is a 150-kD membrane-associated glycoprotein that specifically binds FGFs. CFR protein is not detectable at the cell surface and immunocytochemistry with anti-CFR antibodies demonstrates that CFR is concentrated in the Golgi apparatus. These data suggest CFR does not function as a plasma membrane FGF receptor. CFR expressed in chinese hamster ovary cells reduces the intracellular accumulation of exogenously applied FGF-1 and FGF-2. A mutant CFR lacking the juxtamembrane, transmembrane and intracellular domains is unable to alter intracellular FGF levels. Mutant CFR is detected throughout the cell, indicating that the domains absent in mutant CFR are required for appropriate subcellular localization and the regulation of intracellular FGF levels. Although the activation of plasma membrane receptors is necessary for cellular responses to FGFs, a requirement for intracellular FGF has also been proposed. The subcellular localization of CFR and its ability to regulate the levels of intracellular FGFs suggests that CFR may be involved in intracellular FGF trafficking and the regulation of cellular responses to FGFs.
Zuber
M E
ME
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47906, USA.
Zhou
Z
Z
Burrus
L W
LW
Olwin
B B
BB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive / Q000502:physiology; D001692:Biological Transport / Q000502:physiology; D016466:CHO Cells / Q000502:physiology; D006224:Cricetinae; D003545:Cysteine / Q000502:physiology*; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006056:Golgi Apparatus / Q000737:chemistry; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D017434:Protein Structure, Tertiary; D017468:Receptors, Fibroblast Growth Factor / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013347:Subcellular Fractions / Q000737:chemistry
D007457:Iodine Radioisotopes; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D003545:Cysteine