Isolation of a cDNA encoding the human homolog of COX17, a yeast gene essential for mitochondrial copper recruitment.
The COX17 gene of Saccharomyces cerevisiae codes for a cytoplasmic protein essential for the expression of functional cytochrome oxidase. This protein has been implicated in targeting copper to mitochondria. To determine if Cox17p is present in mammalian cells, a yeast strain carrying a null mutation in COX17 was transformed with a human cDNA expression library. All the respiratory competent clones obtained from the transformations carried a common cDNA sequence with a reading frame predicting a product homologous to yeast Cox17p. The cloning of a mammalian COX17 homolog suggests that the encoded product is likely to function in copper recruitment in eucaryotic cells in general. Its presence in humans provides a possible target for genetically inherited deficiencies in cytochrome oxidase.
Amaravadi
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R
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Glerum
D M
DM
Tzagoloff
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A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D003300:Copper / Q000378:metabolism*; D000079922:Copper Transport Proteins; D018076:DNA, Complementary / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000378:metabolism; D004926:Escherichia coli; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
C100359:COX17 protein, S cerevisiae; C491006:COX17 protein, human; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D000079922:Copper Transport Proteins; D018076:DNA, Complementary; D018832:Molecular Chaperones; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D003300:Copper; D003576:Electron Transport Complex IV