Cytogenetic analysis of gliomas by in situ hybridization of stereotactic biopsy material.
Chromosome analysis of brain tumours can provide important pathobiological data; however, cytogenetic tools are so far not routinely applied for diagnosis. In the present study 25 paraffin embedded stereotactic biopsies from 19 glioma patients were studied using in situ hybridization of chromosome #10 and #15 using biotinylated pericentromeric probes. Numerical changes of chromosome #10 are frequent alterations in glioblastoma. Quantification of chromosome #15 served as a control in order to exclude artificial monosomies or nonspecific changes. The number of chromosomes in at least 200 cells were counted for each specimen. 18 of 25 biopsies could be evaluated quantitatively. The small volume of probes was not a limiting factor for analysis. Quantification of "nonspecific" chromosome #15 revealed single spots in 22-41% of all cells in the 18 biopsies. Chromosome #10 showed single spots in a range between 34 and 44% of counted nuclei in 13/18 biopsies. In 5 out of 18 biopsies 51-60% monosomies were found: in this subgroup were 4 high grade gliomas. These cases were interpreted as monosomy of chromosome #10. The results demonstrate feasibility and quantitative evaluability of cytogenetic analysis in stereotactic biopsy material using in situ hybridization.
Lippitz
B E
BE
Department of Neurosurgery, Medical Faculty, Technical University Aachen, Federal Republic of Germany.
Scheitinger
C
C
Scholz
M
M
Weis
J
J
Gilsbach
J M
JM
Füzesi
L
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000235:genetics / Q000473:pathology; D001706:Biopsy; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations / Q000235:genetics; D002879:Chromosomes, Human, Pair 10; D002884:Chromosomes, Human, Pair 15; D015342:DNA Probes; D005260:Female; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009006:Monosomy; D009837:Oligodendroglioma / Q000235:genetics / Q000473:pathology; D013238:Stereotaxic Techniques
D015342:DNA Probes