title: Birth of a western lowland gorilla (Gorilla gorilla gorilla) following in vitro fertilization and embryo transfer.
abstract: A 21-year-old multiparous female exhibiting 31-41 day menstrual cycles was given hFSH (225 IU/day, Metrodin 75, from cycle day 3 through 9 (menses = day 1) and hCG (10,000 IU, Profasi, on day 10 to stimulate follicular development. At 35 h after hCG, under isoflurane (AErrane) anesthesia, follicles were aspirated by controlled suction under transvaginal ultrasound guidance. Metaphase II oocytes (n = 11) were placed in modified human tubal fluid (mHTF, 100 microliters) medium under oil at 37 degrees C in humidified 5% CO2. Frozen semen, collected by voluntary ejaculation, was thawed (70 degrees C H2O bath, 6 sec), diluted slowly, centrifuged, and resuspended in mHTF, and 160,000 motile spermatozoa/ml were added at 6 h after oocyte recovery. At 21 h postinsemination (p.i.) eight oocytes were at the two-cell stage, five were cryopreserved, and three were cultured to the six- to eight-cell stage in mHTF with granulosa cells before transcervical uterine transfer at 47 h p.i. using a Teflon catheter. Micronized progesterone (400 mg/d) was orally administered for 10 weeks posttransfer (p.t.). Ultrasound examination revealed a single fetus at 15 weeks p.t., and unassisted delivery of a live 1.37 kg female infant occurred at 29 weeks. Am. J. Primatol. 41:247-260, 1997.
authors.affiliation: Cincinnati Zoo and Botanical Garden, Center for Reproduction of Endangered Wildlife, Ohio, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Pope
authors.forename: B L
authors.initials: BL
authors.lastname: Dresser
authors.forename: N W
authors.initials: NW
authors.lastname: Chin
authors.forename: J H
authors.initials: JH
authors.lastname: Liu
authors.forename: N M
authors.initials: NM
authors.lastname: Loskutoff
authors.forename: E J
authors.initials: EJ
authors.lastname: Behnke
authors.forename: C
authors.initials: C
authors.lastname: Brown
authors.forename: M A
authors.initials: MA
authors.lastname: McRae
authors.forename: C E
authors.initials: CE
authors.lastname: Sinoway
authors.forename: M K
authors.initials: MK
authors.lastname: Campbell
authors.forename: K N
authors.initials: KN
authors.lastname: Cameron
authors.forename: O M
authors.initials: OM
authors.lastname: Owens
authors.forename: C A
authors.initials: CA
authors.lastname: Johnson
authors.forename: R R
authors.initials: RR
authors.lastname: Evans
authors.forename: M I
authors.initials: MI
authors.lastname: Cedars
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000662:veterinary; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D006071:Gorilla gorilla; D008597:Menstrual Cycle / Q000502:physiology; D009865:Oocytes; D012662:Semen Preservation / Q000662:veterinary
chemical_list: D005640:Follicle Stimulating Hormone