Detection of colorectal carcinoma cells in circulating peripheral blood by reverse transcription-polymerase chain reaction targeting cytokeratin-20 mRNA.
For the detection of circulating colorectal carcinoma cells, we investigated the presence of cytokeratin 20 (CK 20) mRNA in the peripheral blood of colorectal carcinoma patients. Application of our published technique resulted in analysis by reverse transcription followed by three-step nested polymerase chain reaction. This analysis could detect a single Colo 205 colon cancer cell mixed with 1 ml of blood. Our system also successfully detected the presence of CK 20 mRNA in actual patients' peripheral blood samples. Our highly sensitive and specific system for the detection of CK-20 mRNA from patients' peripheral blood thus seems to be useful for screening for circulating colorectal carcinoma cells.
Funaki
N O
NO
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Tanaka
J
J
Itami
A
A
Kasamatsu
T
T
Ohshio
G
G
Onodera
H
H
Monden
K
K
Okino
T
T
Imamura
M
M
D000328:Adult; D000368:Aged; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D015179:Colorectal Neoplasms / Q000097:blood* / Q000235:genetics* / Q000556:secondary; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics*; D053554:Keratin-20; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012333:RNA, Messenger / Q000097:blood* / Q000235:genetics*; D012334:RNA, Neoplasm / Q000097:blood* / Q000235:genetics*; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D017931:DNA Primers; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D007381:Intermediate Filament Proteins; C507499:KRT20 protein, human; D053554:Keratin-20; D012333:RNA, Messenger; D012334:RNA, Neoplasm