Transient expression of alpha-1B adrenoceptor messenger ribonucleic acids in the rat superior cervical ganglion during postnatal development.
We have examined the developmental profile of the alpha-1 and alpha-2 adrenergic receptor messenger ribonucleic acids expression in the rat superior cervical ganglion. The expression of the six messenger ribonucleic acids was studied using reverse transcription-polymerase chain reaction. At four weeks, the dominant messenger ribonucleic acids transcripts in this sympathetic ganglion were alpha-1C, alpha-2A, alpha-2B and alpha-2C. The expression of alpha-1 genes in the superior cervical ganglion appears to be regulated during postnatal development in that two alpha-1 (alpha-1B, alpha-1C) genes were expressed at birth, three, seven and 14 days postnatal but no amplified product for alpha-1B was detected at 28 days and in the aged animals, while the alpha-1C transcript continued to be expressed. No amplified product for alpha-1D was detected in superior cervical ganglion at any of the ages studied. While all three alpha-2 genes were expressed in the superior cervical ganglion at four weeks the dominant alpha-2 messenger ribonucleic acids transcript expressed in the superior cervical ganglion was alpha-2A. This pattern of alpha-2 adrenoceptor gene expression was maintained from birth, throughout development and into old age. These results suggest that the expression of alpha-1 adrenergic receptors in the superior cervical ganglion is regulated developmentally while the expression of alpha-2 genes remains unchanged.
Vidovic
M
M
Division of Neuroscience, The John Curtin School of Medical Research, The Australian National University, Canberra.
Hill
C E
CE
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D018507:Gene Expression Regulation, Developmental; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011921:Rats, Inbred WKY; D011942:Receptors, Adrenergic, alpha / Q000096:biosynthesis*; D018340:Receptors, Adrenergic, alpha-1 / Q000096:biosynthesis*; D017783:Superior Cervical Ganglion / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic
C547718:Adra1b protein, rat; D012333:RNA, Messenger; D011942:Receptors, Adrenergic, alpha; D018340:Receptors, Adrenergic, alpha-1