Reactive oxygen species as mediators of the transformed phenotype.
Reactive oxygen species (ROS) are known to be involved in different pro- and anticarcinogenic mechanisms. However, their influence on the maintenance of the transformed phenotype has not been studied so far. Here we show that the anchorage-independent growth of transformed murine fibroblasts is inhibited by antioxidants and radical scavengers in a concentration-dependent and reversible manner. These agents also reduce TGF-beta-dependent stimulation of colony formation in soft agar, pointing to their specific interference with TGF-beta-triggered signal chains involved in the maintenance of the transformed state.
Jürgensmeier
J M
JM
Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Germany.
Panse
J
J
Schäfer
R
R
Bauer
G
G
D016475:3T3 Cells / Q000187:drug effects / Q000473:pathology; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D003114:Colony-Forming Units Assay; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D051379:Mice; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000494:pharmacology
D017382:Reactive Oxygen Species; D016212:Transforming Growth Factor beta; D004121:Dimethyl Sulfoxide