Plasminogen activator inhibitor-1 accelerates lung metastasis formation of human fibrosarcoma cells.
We have studied the effects of PAI-1 on the formation of metastasis using human fibrosarcoma cells and DNA transfection. A clone (1-3C), which shows low constitutive expression of PAI-1 and low metastatic potential to the lung, was selected from human fibrosarcoma cell line HT-1080. Newly-derived clones transfected with human PAI-1 cDNA showed a 3-5 fold increase in the antigen level of PAI-1. Further, these clones demonstrated a significant increase in both the number and incidence of lung metastases when inoculated into the tail vein of athymic mice. PAI-1 could be a prognostic marker and a target for understanding the physiology of metastasis, as well as for the treatment and prevention of hematogenous lung metastasis.
Tsuchiya
H
H
Department of Orthopaedic Surgery, School of Medicine, Kanazawa University, Japan.
Sunayama
C
C
Okada
G
G
Matsuda
E
E
Tomita
K
K
Binder
B R
BR
D000818:Animals; D005260:Female; D005354:Fibrosarcoma / Q000473:pathology*; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis
D017395:Plasminogen Activator Inhibitor 1; D014568:Urokinase-Type Plasminogen Activator