title: Human monoclonal antibody 99mTc-88BV59: detection of colorectal cancer, recurrent or metastatic disease and immunogenicity assessment.
abstract: This study presents immunoscintigraphic results in 24 patients suffering from primary colorectal cancer, recurrent or metastatic disease after the injection of 1197-1351 MBq technetium-99m labelled totally human monoclonal antibody 88BV59. Labelling efficacy of 99mTc-88BV59 ranged from 97% to 99%. Immunoscintigraphy was performed 18-20 h after injection. Scintigraphic findings were compared with those of computed tomography (CT). Patients underwent surgery in order to evaluate immunoscintigraphic findings histologically. Sera of the patients (before injection and 1 and 3 months post infusion) were analysed for the presence of human anti-human antibodies (HAHA). None of the patients showed a HAHA response as assessed by a solid-phase ELISA assay. The antibody scan detected about 25% more lesions than CT. In the detection of extrahepatic disease, the sensitivity of the antibody scan proved to be 68%, whereas the sensitivity of CT was 41%.
authors.affiliation: Department of Nuclear Medicine, Johann Wolfgang Goethe University Medical Center, Frankfurt/Main, Germany.
authors.forename: B J
authors.initials: BJ
authors.lastname: Krause
authors.forename: R P
authors.initials: RP
authors.lastname: Baum
authors.forename: E
authors.initials: E
authors.lastname: Staib-Sebler
authors.forename: M
authors.initials: M
authors.lastname: Lorenz
authors.forename: A
authors.initials: A
authors.lastname: Niesen
authors.forename: G
authors.initials: G
authors.lastname: Hör
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D015179:Colorectal Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000000981:diagnostic imaging* / Q000276:immunology; D016719:Radioimmunodetection; D012680:Sensitivity and Specificity; D013667:Technetium; D014057:Tomography, X-Ray Computed
chemical_list: D000911:Antibodies, Monoclonal; D013667:Technetium