[The expression of transforming and epidermal growth factors in organ cultures of embryonic lungs].
Bronchial branching of the early embryonic lung rudiments (Day 12 of pregnancy) cultivated on polycarbonic millipore TM filters was studied on the total preparations. Each lobe of the embryonic lung of the 12-day embryos has three to four terminal buds. After four days of cultivation, their number increased to 18. Immuno-reactive staining of EGF and its receptor was observed both in the cells lining the primitive airway and in the cells and extracellular matrix surrounding the airway of embryonic lung explants cultivated for four and 10 days on TM and MF filters. Expression of TGF-alpha was co-localized with that of EGF but on MF filters only. This mat be connected with the phenomenon of autoinduction.
Popova
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NV
Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow.
Rossi
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L
D000818:Animals; D001980:Bronchi / Q000196:embryology / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism; D005865:Gestational Age; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology / Q000378:metabolism*; D051379:Mice; D008851:Micropore Filters; D009924:Organ Culture Techniques; D016211:Transforming Growth Factor alpha / Q000378:metabolism*
D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D066246:ErbB Receptors
Ekspressiia transformiruiushchego i épidermal'nogo faktorov rosta v organnykh kul'turakh émbrional'nykh legkikh.