title: The presence of GPI-linked protein(s) in an archaeobacterium, Sulfolobus acidocaldarius, closely related to eukaryotes.
abstract: GPI-anchored proteins are distributed ubiquitously in eukaryotes, but not in procaryotes. By metabolic-labeling of Sulfolobus acidocaldarius cells, 14C-radiolabeled precursors of GPI and caldarchaetidylinositol were incorporated into 120, 143 and 185 kDa proteins. The 185 kDa protein was specifically solubilized by bacterial phosphatidylinositol-specific phospholipase C. Therefore, Sulfolobus proved to contain at least one GPI-anchored proteins.
authors.affiliation: Department of Microbial Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: R
authors.initials: R
authors.lastname: Nishizaki
authors.forename: H
authors.initials: H
authors.lastname: Ikezawa
mesh_terms: D019143:Evolution, Molecular; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D010802:Phylogeny; D011506:Proteins / Q000737:chemistry*; D016945:Sulfolobus acidocaldarius / Q000737:chemistry*
chemical_list: D017261:Glycosylphosphatidylinositols; D011506:Proteins