Migration of guinea pig airway epithelial cells in response to bombesin analogues.
Bombesin-like peptides within neuroepithelial cells elicit proliferation of normal and malignant airway epithelial cells. It is not clear that these peptides also elicit epithelial cell migration, a necessary component of airway repair after injury. We studied the effects of the bombesin analogues, gastrin releasing peptide (GRP) and neuromedin B (NMB), on guinea pig tracheal epithelial cell (GPTEC) migration. Primary GPTEC were allowed to migrate through 8-microm-pore gelatin-coated filters for 6 h in a chemotaxis chamber, after which the number of migrated cells per 10 high power fields (10 hpf) were counted. Both neuropeptides elicited migration of GPTEC: 24.8 +/- 4.5 cells for 10(-11) M NMB (P < 0.001 versus control, n = 4) and 16.8 +/- 1.2 cells for 10(-12) M GRP (P < 0.001 versus control, n = 8). Migration was attenuated substantially by a bombesin receptor antagonist. To investigate further the relationship of migration through a filter to the repair of a damaged epithelium, we studied the repair of epithelial cells by video microscopy. A 0.3- to 0.5-microm2 wound was created in a confluent monolayer of GPTEC, and wound closure was followed over 24 h. There was no significant acceleration in the rate of repair of GRP- or NMB-stimulated monolayers compared to control. These data demonstrate that GRP and NMB elicit migration of airway epithelial cells but may not play a significant role in the early repair of the airway epithelium in culture.
Kim
J S
JS
Department of Medicine, University of Chicago, Illinois 60637, USA.
McKinnis
V S
VS
White
S R
SR
D000818:Animals; D001839:Bombesin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D002633:Chemotaxis / Q000502:physiology*; D004847:Epithelial Cells; D019886:Gastrin-Releasing Peptide; D006020:Glycopeptides / Q000494:pharmacology; D006168:Guinea Pigs; D007328:Insulin / Q000494:pharmacology; D008297:Male; D018715:Microscopy, Video; D015260:Neprilysin / Q000037:antagonists & inhibitors; D015287:Neurokinin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology; D018004:Receptors, Bombesin / Q000037:antagonists & inhibitors; D014945:Wound Healing / Q000502:physiology*
D006020:Glycopeptides; D007328:Insulin; D010455:Peptides; D011480:Protease Inhibitors; D018004:Receptors, Bombesin; C057644:bombesin, Leu(13)-psi-CH2NH-Leu(14)-; D019886:Gastrin-Releasing Peptide; D015287:Neurokinin B; C038393:neuromedin B; D015260:Neprilysin; D001839:Bombesin; C008890:phosphoramidon