[A novel method of subtraction cDNA hybridization allowing cloning of genes showing differential expression in cell micropopulations].
A novel, efficient and simple technique that combines subtractive hybridization with kinetic enrichment is proposed for obtaining enriched cDNA. The method is based on the use of a set of special primers that allow for the selective amplification by PCR only of differentially distributed sequences. Using the proposed technique, cDNA of a new gene XEp-1, specifically expressed in the presumptive epidermis of Xenopus laevis, was cloned, starting with the stage of midgastrula.
Vasil'ev
O L
OL
Luk'ianov
S A
SA
Beliavskiĭ
A V
AV
Kazanskaia
O V
OV
Zaraĭskiĭ
A G
AG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D005801:Genes, Homeobox; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D014982:Xenopus laevis
D018076:DNA, Complementary
Novyĭ metod vychitaiushcheĭ gibridizatsii kDNK, pozvoliaiushchiĭ klonirovat' geny, differentsial'no ékspressiruiushchiesia v mikropopuliatsiiakh kletok.