title: Development of PCR-SSOP for the identification of HLA-A*02 subtypes and determination of HLA-A*02 frequencies within different ethnic populations.
abstract: A PCR-SSOP typing method, involving a single PCR amplification in conjunction with 19 digoxigenin labelled oligonucleotide probes, has been developed for the identification of 17 known HLA-A*02 alleles. The method has been applied to four populations (Northern Ireland, Singapore Chinese, Shetland Island and Mexican) and percentages of HLA-A*02 alleles determined within each population.
authors.affiliation: Northern Ireland Tissue Typing Laboratory, City Hospital, Belfast, Northern Ireland, United Kingdom.
authors.forename: F
authors.initials: F
authors.lastname: Williams
authors.forename: D
authors.initials: D
authors.lastname: Middleton
authors.forename: D
authors.initials: D
authors.lastname: Savage
authors.forename: C
authors.initials: C
authors.lastname: Gorodezky
authors.forename: D W
authors.initials: DW
authors.lastname: Wilson
authors.forename: J M
authors.initials: JM
authors.lastname: Fitzgerald
authors.forename: S J
authors.initials: SJ
authors.lastname: Urbaniak
mesh_terms: D000483:Alleles; D002460:Cell Line; D005006:Ethnicity; D005787:Gene Frequency; D015789:HLA-A2 Antigen / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing; D016133:Polymerase Chain Reaction
chemical_list: D015789:HLA-A2 Antigen