Effect of drying conditions on the taxane content of the needles of ornamental Taxus.
The effect of drying intact clippings of Taxus on the recovery of taxol and related compounds was studied under different drying conditions which included tobacco drying barn, greenhouse, shadehouse, air conditioned laboratory, oven, and freeze-drying. For clippings dried under tobacco barn, greenhouse, oven, and freeze-drying conditions, nearly total recovery of the expected levels based upon projections from analysis of fresh biomass was observed for taxol and cephalomannine. However, only 75-80% of the expected values for 10-deacetyltaxol and 10-deacetylbaccatin III were found. When the length of drying was extended up to 10 and 15 days as in the shadehouse and laboratory conditions, the recovery of all taxanes was adversely affected.
el Sohly
H N
HN
National Center for the Development of Natural Products, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University 38677, USA.
Croom
E M
EM
el Kashoury
E A
EA
Joshi
A S
AS
Kopycki
W J
WJ
McChesney
J D
JD
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003890:Desiccation; D014197:Trees / Q000737:chemistry*
D000470:Alkaloids