Oronasal leishmaniasis caused by a parasite with an unusual isoenzyme profile.
A 45-year-old Sudanese man from western Sudan presented with oronasal leishmaniasis of three years duration. He had no history of previous kala-azar or cutaneous leishmaniasis. The parasite isolated from the oral mucosa was characterized by isoenzymes using 12 enzymes and by polymerase chain reaction amplification of kinetoplast DNA using species-specific primers. The specific primers gave products indistinguishable from those of the Leishmania donovani complex. However, the isoenzyme profile showed a zymodeme pattern that was significantly different from the zymodemes previously reported in the Sudan and the Ethiopian region.
Ibrahim
M
M
Institute of Endemic Diseases, University of Khartoum, Sudan.
Suliman
A
A
Hashim
F A
FA
Khalil el-T
A
A
Evans
D A
DA
Kharazmi
A
A
el Hassan
A M
AM
D000818:Animals; D017931:DNA Primers; D018105:DNA, Kinetoplast / Q000032:analysis; D004592:Electrophoresis, Starch Gel; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007891:Leishmania / Q000145:classification / Q000201:enzymology*; D007893:Leishmania donovani / Q000145:classification / Q000235:genetics; D007896:Leishmaniasis / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000469:parasitology* / Q000473:pathology; D009297:Nasal Mucosa / Q000469:parasitology / Q000473:pathology; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013397:Sudan
D017931:DNA Primers; D018105:DNA, Kinetoplast; D007527:Isoenzymes