title: Detection of polymorphisms in the human urokinase-type plasminogen activator gene.
abstract: Expressed polymorphisms in the genes encoding components of the fibrinolytic cascade could have implications for the predisposition to thrombolytic disorders and/or for tumor metastasis. The occurrence of published two amino acid sequences at position 194 of the human urokinase-type plasminogen activator prompted us to search by SSCP for frequent polymorphisms in several exons of the gene. Surprisingly, only one sequence was detected in codon 194 (> 200 alleles). Two polymorphisms were observed in this study: the most frequent one, a C to T change near the beginning of exon 8, is probably silent; a less frequent polymorphism results in the replacement of a Leu residue by a Pro, in the kringle domain.
authors.affiliation: Département de Pathologie, Centre médical universitaire, Geneva, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Conne
authors.forename: M
authors.initials: M
authors.lastname: Berczy
authors.forename: D
authors.initials: D
authors.lastname: Belin
mesh_terms: D000483:Alleles; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
chemical_list: D014568:Urokinase-Type Plasminogen Activator