Histochemical study on xanthine oxidase activity in the normal rabbit cornea and lens and after repeated irradiation of the eye with UVB rays.
In the normal rabbit cornea and lens the activity of xanthine oxidase, an enzyme belonging to oxidases generating reactive oxygen species (ROS), is present in the corneal epithelium as well as endothelium and lens epithelium. Repeated irradiation of the eyes with UVB rays (5 min 1 x daily, for 1 to 4 days) caused a gradual increase of xanthine oxidase activity, particularly in the corneal epithelium. Application of catalase, a scavenger of hydrogen peroxide, to the eye surface during the irradiation diminished the increase of xanthine oxidase activity. On the contrary, the pretreatment of the rabbit eyes with 3-aminotriazole, an inhibitor of catalase, for 3 days before the irradiation enhanced the increase of xanthine oxidase activity. In comparison to untreated eyes, protracted irradiation of the eyes with UVB rays (up to 10 days) caused a decrease of xanthine oxidase activity in the same cell layers of the cornea and lens. It is suggested that xanthine oxidase is involved in the generation of ROS in the anterior eye segment during early irradiation of the eyes with UVB rays and participates in its damage. Prolonged repeated irradiation of the eye (5 min 1 x daily for 5 to 10 days) caused a decrease of xanthine oxidase activity in the cornea and lens which is attributed to profound damage of the whole anterior eye segment.
Cejkova
J
J
Laboratory of Biochemistry and Histochemistry, Institute of Experimental Medicine, Academy of Sciences of Czech Republic.
Lojda
Z
Z
D000640:Amitrole / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002682:Chinchilla; D003315:Cornea / Q000201:enzymology* / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D006651:Histocytochemistry; D007908:Lens, Crystalline / Q000201:enzymology* / Q000528:radiation effects; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays; D014969:Xanthine Oxidase / Q000378:metabolism* / Q000528:radiation effects
D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D002374:Catalase; D014969:Xanthine Oxidase; D000640:Amitrole