Sequence heterogeneity of an mpb70 gene analogue in Mycobacterium kansasii.
The protein antigen MPB70 is a major component of culture supernatants of Mycobacterium bovis and is an active ingredient of bovine PPD used for skin-testing cattle for tuberculosis. We have shown that Mycobacterium kansasii possesses a similar gene that cross-reacts in a PCR test for M. bovis. Single strand conformational polymorphism analysis, and the DNA sequence of the PCR product, shows differences between M. kansasii strains, supporting the suggestion that M. kansasii is not a homogeneous species.
Woolford
A J
AJ
Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, UK.
Hewinson
R G
RG
Woodward
M
M
Dale
J W
JW
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D002417:Cattle; D018740:Genetic Heterogeneity; D008969:Molecular Sequence Data; D009170:Nontuberculous Mycobacteria / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014380:Tuberculosis, Bovine / Q000235:genetics*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C060216:MPB70 protein, Mycobacterium bovis