Chronic ethanol enhances NMDA-induced AP-1 activity in cultured rat cerebellar granule cells.
We examined the effects of chronic ethanol exposure (50 mM; 3 days) on N-methyl-D-aspartate (NMDA)- and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-induced AP-1 transcription factor DNA binding activity in primary cultures of rat cerebellar granule cells. Chronic ethanol exposure enhanced NMDA-stimulated AP-1 binding activity, with no corresponding change in AMPA-stimulated AP-1 binding. Supershift analysis with specific antibodies against the members of Fos and Jun protein families showed that the NMDA-induced AP-1 protein complex consisted predominantly of c-Fos and Jun D proteins. Chronic ethanol treatment by itself did not change the protein composition of the AP-1 complex.
Cebers
G
G
Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden.
Hou
Y N
YN
Cebere
A
A
Terenius
L
L
Liljequist
S
S
D000818:Animals; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004357:Drug Synergism; D004586:Electrophoresis; D000431:Ethanol / Q000494:pharmacology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D007118:Immunoassay; D016202:N-Methylaspartate / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000187:drug effects; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects; D018808:Transcription Factor AP-1 / Q000096:biosynthesis*
D002492:Central Nervous System Depressants; D018690:Excitatory Amino Acid Agonists; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D018808:Transcription Factor AP-1; D000431:Ethanol; D016202:N-Methylaspartate