title: Arbitrary primed PCR rules out Clostridium difficile cross-infection among patients in a haematology unit.
abstract: Eight out of 20 (40%) patients with haematological malignancies hospitalized in the same unit of our hospital from 24 January to 24 April 1995, suffered from diarrhoea due to Clostridium difficile. The C. difficile isolates were characterized by serotyping and by arbitrary primed polymerase chain reaction (AP-PCR) using three different 10-mer oligonucleotides. It was found by serotyping that five patients had non-typeable isolates and three had serogroup H isolates. The AP-PCR typed all the isolates and yielded various patterns suggesting that there had been no cross-transmission between the patients. Control faecal sample cultures showed that two patients were still carrying the same isolates after specific treatment with vancomycin or metronidazole, and that one patient had acquired an isolate with a new AP-PCR type. AP-PCR was found to be a rapid, effective discriminative method for the immediate epidemiological tracking of hospital-acquired infections due to C difficile.
authors.affiliation: Service de Microbiologie, Hôpital Beaujon, Clichy, France.
authors.forename: F
authors.initials: F
authors.lastname: Lemann
authors.forename: C
authors.initials: C
authors.lastname: Chambon
authors.forename: F
authors.initials: F
authors.lastname: Barbut
authors.forename: C
authors.initials: C
authors.lastname: Gardin
authors.forename: J
authors.initials: J
authors.lastname: Brière
authors.forename: N
authors.initials: N
authors.lastname: Lambert-Zechovsky
authors.forename: C
authors.initials: C
authors.lastname: Branger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016360:Clostridioides difficile / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003428:Cross Infection / Q000382:microbiology*; D003967:Diarrhea / Q000382:microbiology*; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology; D005260:Female; D019337:Hematologic Neoplasms / Q000150:complications*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping / Q000379:methods