A protein inhibitor of hepatic drug metabolizing enzymes from Ehrlich ascites cells.
Hepatic drug metabolizing enzymes were significantly decreased in Ehrlich ascites tumour-bearing mice. A protein inhibitor of hepatic drug metabolizing enzymes was isolated from Ehrlich ascites cells and purified. This involved ammonium sulphate fractionation (60-80%), DEAE, phosphocellulose, Sephadex G-100 and hydroxyapatite column chromatography. Purification attained was 800-fold. The inhibitory protein was effective in decreasing all the components of hepatic mixed-function-oxidase system and drug metabolizing enzymes both in vivo and in vitro. This novel inhibitor may have potential applications in chemical carcinogenesis.
Kamat
J P
JP
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Mumbai, India.
D000633:Aminopyrine N-Demethylase / Q000037:antagonists & inhibitors; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology / Q000378:metabolism*; D065607:Cytochrome P-450 Enzyme Inhibitors; D015786:Cytochromes b5 / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D005260:Female; D014453:Glucuronosyltransferase / Q000037:antagonists & inhibitors; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors*; D009251:NADPH-Ferrihemoprotein Reductase / Q000037:antagonists & inhibitors
D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D015786:Cytochromes b5; D006899:Mixed Function Oxygenases; D000633:Aminopyrine N-Demethylase; D009251:NADPH-Ferrihemoprotein Reductase; D014453:Glucuronosyltransferase