title: Comparison of two pseudorabies virus vaccines, that differ in capacity to reduce virus excretion after a challenge infection, in their capacity of reducing transmission of pseudorabies virus.
abstract: Pseudorabies virus (PRV) vaccines are often compared for their capacity to reduce virus excretion after a challenge infection. Vaccines, used for the eradication of PRV, however, should reduce transmission of PRV among pigs. The purpose of this study was to investigate whether the amount of virus excreted after a challenge infection is an accurate measure of the capacity of a vaccine to reduce transmission of PRV among pigs. Two experiments were carried out, each using two groups of 10 pigs. The pigs in group one were intramuscularly vaccinated once with the glycoprotein E (gE)-negative vaccine X, the pigs in group two with the gE-negative strain 783. Eight weeks later, 5 pigs in each group were inoculated with wild-type PRV. A gE-ELISA was used to detect PRV infection. The transmission of PRV was estimated from the number of contact infections and expressed as the reproduction ratio R. The inoculated pigs vaccinated with vaccine X shed significantly more virus than the inoculated pigs vaccinated with strain 783. However, despite the difference in virus excretion, the transmission of PRV between the two groups did not differ. We conclude that virus excretion is not an accurate measure for determining vaccine effectiveness. However, R of vaccine X (R = 0.98) was not significantly below one, whereas R of vaccine 783 (R = 0) was significantly below one. Consequently, we cannot exclude the possibility that major outbreaks of PRV occur among pigs vaccinated with vaccine X.
authors.affiliation: DLO-Institute of Animal Science and Health (ID-DLO), Lelystad, The Netherlands. a.bouma@id.dlo.nl
authors.forename: A
authors.initials: A
authors.lastname: Bouma
authors.forename: M C
authors.initials: MC
authors.lastname: De Jong
authors.forename: T G
authors.initials: TG
authors.lastname: Kimman
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000821:virology; D002478:Cells, Cultured; D011558:Herpesvirus 1, Suid / Q000302:isolation & purification*; D007668:Kidney; D009960:Oropharynx / Q000821:virology; D011557:Pseudorabies / Q000517:prevention & control* / Q000635:transmission*; D022421:Pseudorabies Vaccines; D012044:Regression Analysis; D013552:Swine; D014765:Viral Vaccines; D017201:Virus Shedding
chemical_list: D022421:Pseudorabies Vaccines; D014765:Viral Vaccines