[Inflammatory cytokine mediated anti-anabolic effects: a potential mechanism in rheumatoid cartilage degeneration].
Increased levels of inflammatory cytokines such as II-1 and TNF-alpha are described in rheumatoid and osteoarthritic synovial fluid. These mediators are also very well established anti-anabolic modulators of chondrocyte synthetic activity in vitro. Our study aimed to investigate, whether chondrocytes in rheumatoid and osteoarthritic cartilage in situ show reaction pattern compatible with the putative effects of these modulatory agents. Immunohistochemical analysis using type II collagen specific antibodies showed considerable loss of staining in many sites of osteoarthritic and rheumatoid articular cartilage. mRNA analysis showed besides an overall activation of synthetic activity in rheumatoid and osteoarthritic cartilage, a decreased expression of cartilage matrix proteins in the upper zone. The cease of the anabolic activity of rheumatoid and osteoarthritic chondrocytes and the increased catabolism of matrix components contributes to the anabolic-catabolic imbalance in rheumatoid and osteoarthritic cartilage and is suggestive to be a crucial event in the progress of the disease. It correlates well to the putative anti-anabolic effect of inflammatory cytokines such as II-1 and TNF-alpha and could indicate a potential role of these mediators in rheumatoid and osteoarthritic cartilage destruction.
Aigner
T
T
Pathologisches Institut, Universität, Erlangen.
Vornehm
S I
SI
Belke
J
J
von der Mark
K
K
Kirchner
T
T
D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000473:pathology*; D002358:Cartilage, Articular / Q000276:immunology / Q000473:pathology*; D003094:Collagen / Q000032:analysis; D016207:Cytokines / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D007249:Inflammation; D007375:Interleukin-1 / Q000502:physiology; D010003:Osteoarthritis / Q000276:immunology / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D016207:Cytokines; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D003094:Collagen
Entzündungsmediatoren-vermittelte anti-anabolische Effekte: ein potentieller Mechanismus der rheumatischen Knorpeldegeneration.