title: Synthesis of methyl 6-O-beta-inulotriosyl-alpha-D-glucopyranoside by intermolecular transglycosylation reaction of cycloinulo-oligosaccharide fructanotransferase.
abstract: Incubation of cycloinulohexaose and methyl alpha-D-glucopyranoside in the presence of cycloinulo-oligosaccharide fructanotransferase gave some hetero-oligosaccharides. The main product was a tetrasaccharide whose sugar composition was methyl alpha-D-glucopyranoside-D-fructose in a ratio 1:3. This oligosaccharide was isolated from the reaction mixture by charcoal-column chromatography and was identified as methyl O-beta-D-fructofuranosyl-(2--> 1)-O-beta-D-fructofuranosyl-(2-->1)-O-beta-D-fructofuranosyl-(2-->6)- alpha-D-glucopyranoside (methyl 6-O-beta-inulotriosyl-alpha-D-glucopyranoside), by two-dimensional NMR spectroscopy.
authors.affiliation: Department of Biology, Osaka Kyoiku University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawamura
authors.forename: H
authors.initials: H
authors.lastname: Nakai
authors.forename: T
authors.initials: T
authors.lastname: Uchiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
authors.forename: M
authors.initials: M
authors.lastname: Sawada
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006602:Hexosyltransferases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D009005:Monosaccharides; D009844:Oligosaccharides; C102956:cycloinulohexaose CF6; C105302:methyl 6-O-inulotriosylglycopyranoside; D006602:Hexosyltransferases; C062589:cycloinulo-oligosaccharide fructanotransferase