title: Syndet is a novel SNAP-25 related protein expressed in many tissues.
abstract: SNAP-25 is a synaptosomal associated protein localized at the plasma membrane of nerve terminals. SNAP-25 associates with syntaxin 1 and vesicle-associated membrane protein-2 (VAMP-2) and is thought to form a complex essential for neurotransmitter release. We have identified syndet, a novel protein related to the family of SNAP-25 isoforms. Like SNAP-25, syndet has regions with high probability of forming coiled coils, a cysteine rich-domain, and lacks a signal sequence or transmembrane domains. Syndet is tightly bound to membranes, possibly by acylation within the cysteine-rich domain. Syndet is expressed in non-neuronal tissues. In adipocytes, syndet is found at the plasma membrane and in an intracellular compartment. The identification of syndet supports the hypothesis that multiple SNAP-25 related proteins ensure specificity of vesicle fusion at the cell surface.
authors.affiliation: Department of Anatomy and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA.
authors.forename: G
authors.initials: G
authors.lastname: Wang
authors.forename: J W
authors.initials: JW
authors.lastname: Witkin
authors.forename: G
authors.initials: G
authors.lastname: Hao
authors.forename: V A
authors.initials: VA
authors.lastname: Bankaitis
authors.forename: P E
authors.initials: PE
authors.lastname: Scherer
authors.forename: G
authors.initials: G
authors.lastname: Baldini
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D050825:Synaptosomal-Associated Protein 25
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C494208:SNAP25 protein, human; C107017:Snap23 protein, mouse; C494209:Snap25 protein, mouse; D050825:Synaptosomal-Associated Protein 25