Systematic multivariate optimization of separation of auxins by capillary electrophoresis based on overlapping resolution mapping scheme.
In this paper, the overlapping resolution mapping (ORM) scheme in the separation of 15 plant auxins by capillary electrophoresis. An interpretative optimization approach was utilised to predict the optimum buffer pH, and the concentrations of alpha-, beta-, and gamma-cyclodextrins. A four-dimensional overlapping resolution mapping scheme was developed to optimize these four parameters simultaneously. By conducting sixteen prelanned experiments, global optimum conditions for the separation within an acceptable analysis time could be obtained.
Liu
J
J
Department of Chemistry, National University of Singapore, Republic of Singapore.
Li
S F
SF
D002021:Buffers; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry*; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins
D002021:Buffers; D003505:Cyclodextrins; D007210:Indoleacetic Acids; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; C031215:betadex; C023792:gamma-cyclodextrin; C032613:alpha-cyclodextrin