Interactions of 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene with mitochondrial oxidative phosphorylation.
The effects of DDE (2,2-bis(p-chlorophenyl)-1,1-dichloroethylene), the major metabolite of DDT (2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane), on rat liver mitochondrial bioenergetic activities were examined. The approach developed by M. D. Brand (Biochim Biophys Acta 1018: 128-133, 1990) was used to assess the effects of DDE because it is possible to discriminate the sites of action of compounds having pleiotypic effects on oxidative phosphorylation. Data were further confirmed using a "classical" approach, including measurements of transmembrane potential, respiratory indexes, enzymatic activities and membrane permeability to protons. DDE up to 40 nmol/mg protein affected the proton motive force generating system. In fact, DDE interacted with succinate dehydrogenase (complex II), decreasing respiration and membrane potential. In this concentration range, the permeability of the inner membrane to protons remained intact. Only higher concentrations (> or = 80 nmol/mg) increased permeability to protons, uncoupling oxidation from phosphorylation. The phosphorylative system was not affected because the rate of ATP synthesis was unchanged. In addition, data from carbonyl cyanide m-chlorophenylhydrazone-uncoupled rotenone-inhibited preparations or submitochondrial particles indicated that F0F1 ATPase activity is not affected by DDE. Therefore, DDE inhibition of complex II and putative inhibition of succinate translocation explain the depression of mitochondrial respiration. The use of appropriate substrates and assay conditions indicates that complexes I, III and IV were not affected by DDE. The uncoupling of oxidative phosphorylation at high concentrations (> 80 nmol DDE/mg protein) was probably related to deleterious effects on the integrity of the mitochondrial membrane. We confirmed that the technique originally proposed by Brand is useful for characterizing the effects of xenobiotics on oxidative phosphorylation. In addition, data provided by this technique closely agree with data from classical studies.
Ferreira
F M
FM
Department of Zoology, University of Coimbra, Portugal.
Madeira
V M
VM
Moreno
A J
AJ
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D003633:Dichlorodiphenyl Dichloroethylene / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D003633:Dichlorodiphenyl Dichloroethylene; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000255:Adenosine Triphosphate