Albumin standards and the measurement of serum albumin with bromcresol green. 1971.
A rapid and reliable method for measuring serum albumin employing bromcresol green is described. The addition of albumin to a solution of bromcresol green in a 0.075 M succinate buffer pH 4.20 results in an increase in absorbance at 628 nm. The absorbance-concentration relationship is linear for samples containing up to 6 g/dl albumin. Bilirubin, moderate lipemia, and salicylate do not interfere with the analysis. The use of nonionic surfactant (Brij-35) reduces the absorbance of the blank, prevents turbidity and provides linearity. The results by this method agree very well with those obtained by electrophoresis and salt fractionation. The method is simple, it has excellent precision and the reagents are stable. A protein standard is introduced which can be employed for both the total serum proteins and albumin determinations.
Dumas
B T
BT
Department of Clinical Pathology, School of Medicine, University of Alabama, Birmingham 35233, USA.
Watson
W A
WA
Biggs
H G
HG
D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D001961:Bromcresol Green; D003124:Colorimetry / Q000266:history; D049673:History, 20th Century; D006801:Humans; D012015:Reference Standards; D012709:Serum Albumin / Q000032:analysis*; D013053:Spectrophotometry / Q000266:history
D012709:Serum Albumin; D001961:Bromcresol Green