Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution.
A simple method for the isolation of alpha-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen alpha-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (> 95%) and purity (> 98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods e.g. chromatographic procedures.
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Institut für Medizinische Molekularbiologie, Medizinische, Universität zu Lübeck, Germany.
Brinckmann
J
J
Behrens
P
P
Müller
P K
PK
Bätge
B
B
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006901:Hydroxylysine / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D010434:Pepsin A / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013552:Swine
D000596:Amino Acids; D006901:Hydroxylysine; D012967:Sodium Dodecyl Sulfate; D003094:Collagen; D010434:Pepsin A; D006909:Hydroxyproline