title: Assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for the presence of bai-like genes.
abstract: Eubacterium sp. strain VPI 12708 has several bile acid-inducible (bai) genes which encode enzymes in the bile acid 7 alpha-dehydroxylation (7 alpha DeOH) pathway. Twelve 7 alpha DeOH-positive intestinal bacterial strains were assayed for 7 alpha DeOH activity, and 13 strains were tested for hybridization with bai genes. Cholic acid 7 alpha DeOH activity varied greatly (> 100-fold) among these strains. Southern blot experiments showed that DNA prepared from 7 of 13 strains hybridized with at least one of the bai genes from Eubacterium sp. strain VPI 12708.
authors.affiliation: Department of Microbiology and Immunology, Virginia Commonwealth University-Medical College of Virginia, Richmond 23298-0678, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Doerner
authors.forename: F
authors.initials: F
authors.lastname: Takamine
authors.forename: C P
authors.initials: CP
authors.lastname: LaVoie
authors.forename: D H
authors.initials: DH
authors.lastname: Mallonee
authors.forename: P B
authors.initials: PB
authors.lastname: Hylemon
mesh_terms: D015139:Blotting, Southern; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism*; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism; D005051:Eubacterium / Q000235:genetics* / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005798:Genes, Bacterial; D006900:Hydroxylation
chemical_list: D002793:Cholic Acids; D019826:Cholic Acid