title: A novel acute lymphoid leukaemia type BCR/ABL transcript in chronic myelogenous leukaemia.
abstract: Using a reverse transcription-polymerase chain reaction (RT-PCR), we identified a patient with typical clinical features of chronic myelogenous leukaemia (CML) in the chronic phase who showed no amplification of the CML-type BCR/ABL transcript. RT-PCR with primers detecting the acute lymphoid leukaemia (ALL)-type transcript disclosed a novel fragment co-amplified with an ALL-type fragment. Sequencing revealed the novel transcript to be a chimaeric mRNA produced by fusion of a segment of BCR exon 2 (e2) to ABL exon 2 (a2), with a 21 base-pair insertion of ABL intron 1b sequence between them. This transcript has not been reported previously.
authors.affiliation: The Third Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: M
authors.initials: M
authors.lastname: Karasawa
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: H
authors.initials: H
authors.lastname: Ogura
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: T
authors.initials: T
authors.lastname: Naruse
mesh_terms: D000328:Adult; D002678:Chimera; D016313:Genes, abl / Q000235:genetics*; D006801:Humans; D015466:Leukemia, Myeloid, Chronic-Phase / Q000150:complications / Q000235:genetics*; D008297:Male; D015513:Oncogene Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000150:complications / Q000235:genetics*; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D014158:Transcription, Genetic
chemical_list: D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr