The effect of alpha-trinositol (D-myo-inositol 1,2,6-trisphosphate) on formalin-evoked spinal amino acid and prostaglandin E2 levels.
The effect of the inositol trisphosphate analog alpha-trinositol on noxious-evoked behavior, amino acid and prostaglandin E2 (PGE2) release was examined in unanesthetized rats using intrathecal microdialysis probes. Subcutaneous injection of 50 microliters 5% formalin solution produced two phases of pain-like behavior and significant elevation of glutamate, aspartate, glycine, taurine and serine during phase 1. PGE2 concentrations were increased during both phases 1 and 2. Intraperitoneal delivery of 300 mg/kg alpha-trinositol significantly suppressed both phases 1 and 2 of formalin-induced behavior and the associated elevation of amino acids and PGE2. These data demonstrate that the antinociceptive effect of alpha-trinositol corresponds to suppression of noxious-evoked release of amino acids and PGE2 from the spinal cord.
Malmberg
A B
AB
Department of Physiology and Pharmacology, University of Göteborg, Sweden. annikam@phy.ucsf.edu
Hedner
T
T
Fallgren
B
B
Calcutt
N A
NA
D000596:Amino Acids / Q000134:cerebrospinal fluid*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000134:cerebrospinal fluid / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D015232:Dinoprostone / Q000134:cerebrospinal fluid*; D005260:Female; D005557:Formaldehyde; D007295:Inositol Phosphates / Q000134:cerebrospinal fluid / Q000494:pharmacology*; D017551:Microdialysis; D010147:Pain Measurement / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*
D000596:Amino Acids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D007295:Inositol Phosphates; D005557:Formaldehyde; D015232:Dinoprostone; C059368:atrinositol