[The DNA-binding activity of the membrane protein annexin II and its interaction with antibodies to the chromatin nuclear ribonucleoprotein (alpha-RNP)].
By screening with labeled Alu DNA, a clone was isolated from cDNA expression library, which appeared identical in sequence to the well-known Ca-phospholipid-binding protein annexin II. To evidence the DNA-binding activity of recombinant annexin II and its presence in the cell nucleus, we have expressed full-length mouse annexin II cDNA in bacteria with pGEMEX vector. The expressed protein was studied with electrophoretic mobility shift assay and for its reaction with polyclonal antibody to chromatin-associated ribonucleoprotein (alpha-RNP), which is one of the major acid-dissolvent components of the nucleus. The obtained results confirm the DNA-binding activity of recombinant annexin II. Annexin II reacts with polyclonal antibody to rat alpha-RNP. So, annexin II is a major nuclear DNA-binding protein in mammalian cells.
Krutilina
R I
RI
Babich
V S
VS
Kropotov
A V
AV
Mudrak
O S
OS
Chesnokov
I N
IN
Turoverova
L V
LV
Konstantinova
I M
IM
Tomilin
N V
NV
D000818:Animals; D017306:Annexin A2 / Q000378:metabolism*; D000906:Antibodies / Q000378:metabolism; D001483:Base Sequence; D002843:Chromatin / Q000276:immunology*; D004247:DNA / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000276:immunology* / Q000302:isolation & purification
D017306:Annexin A2; D000906:Antibodies; D002843:Chromatin; D004274:DNA, Recombinant; D008565:Membrane Proteins; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D004247:DNA
DNK-sviazyvaiushchaia aktivnost' primembrannogo belka anneksina II i ego vzaimodeĭstvie s antitelami k iadernomu ribonukleoteinu khromatina (al'fa-RNP).