title: Melatonin inhibits growth of cultured human uveal melanoma cells.
abstract: The effects of melatonin on the growth of human uveal melanoma cells were studied in vitro. Three continuous uveal melanoma cell lines were tested. Cells were plated into multi-well plates. After 24 h, melatonin was added to the medium at concentrations from 0.001 to 1000 nM. Cells were collected and counted after 5 days and compared with the controls. Melatonin inhibited the growth of melanoma cells in a dose-dependent manner (0.1-10 nM) with a mean inhibition rate of 50%. The uptake of bromodeoxyuridine (BrdU) by the melanoma cells was also measured. Melatonin inhibited the uptake of BrdU of melanoma cells at concentrations of 0.1-10 nM with a mean inhibition rate of 40%. These results indicate that melatonin may offer a new treatment for metastatic uveal melanoma.
authors.affiliation: Department of Pathology and Laboratory Medicine, New York Eye and Ear Infirmary, New York 10003, USA.
authors.forename: D N
authors.initials: DN
authors.lastname: Hu
authors.forename: J E
authors.initials: JE
authors.lastname: Roberts
mesh_terms: D001973:Bromodeoxyuridine / Q000493:pharmacokinetics; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D008545:Melanoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D008550:Melatonin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D001973:Bromodeoxyuridine; D008550:Melatonin