title: Cyclosporin A-induced changes in nitric oxide synthase activity in the rat kidney: in vitro and in vivo assay.
abstract: Cyclosporin A (CsA) is a cyclic undecapeptide that has been used extensively as an immunosuppressive drug in transplantation medicine and is known to interact with L-arginine dependent pathways. We studied the in vitro and in vivo effects of CsA on nitric oxide synthase (NOS) activity in the rat kidney. CsA in concentrations of 44-2200 nM in vitro, and 12.5 or 25 mg/kg body weight per 4 weeks treated rat kidneys in vivo, significantly stimulated NOS activity. CsA may alter the Ca2+/Cam-dependent NOS activity by interacting with rat kidney Ca2+/calmodulin dependent events.
authors.affiliation: Department of Medicine, University of Mississippi Medical Center, Jackson 39216, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Rao
authors.forename: V K
authors.initials: VK
authors.lastname: Kanji
authors.forename: V V
authors.initials: VV
authors.lastname: Setty
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: A K
authors.initials: AK
authors.lastname: Salahudeen
authors.forename: S K
authors.initials: SK
authors.lastname: Das
authors.forename: H H
authors.initials: HH
authors.lastname: Cohly
mesh_terms: D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism; D008297:Male; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007166:Immunosuppressive Agents; D011506:Proteins; D016572:Cyclosporine; D019001:Nitric Oxide Synthase