The cowpea mosaic virus RNA 1-encoded 112 kDa protein may function as a VPg precursor in vivo.
Processing of the 112 kDa ('112K') protein encoded by cowpea mosaic virus RNA 1 was examined in cowpea mesophyll protoplasts using a transient expression system. Cleavage of the 112K protein occurred via two alternative pathways either into VPg and 110K (24K + 87K) or into 26K (VPg + 24K) and 87K proteins. The 26K protein can be further cleaved into VPg and 24K proteins. The results support a model in which the 112K protein functions as the precursor of VPg during initiation of replication.
Peters
S A
SA
Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.
Mesnard
J M
JM
Kooter
I M
IM
Verver
J
J
Wellink
J
J
van Kammen
A
A
D017798:Comovirus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D008970:Molecular Weight; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D011523:Protoplasts / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry*; D014162:Transfection; D014758:Viral Core Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014779:Virus Replication
D011498:Protein Precursors; D012367:RNA, Viral; D014758:Viral Core Proteins