Daidzein stimulation of bone resorption in pit formation assay.
We found that daidzein stimulated bone resorption in the pit formation assay at the concentration of 10(-8)-10(-10) M. On the other hand, genistein and ipriflavone at these concentrations did not have any effect on pit formation. We speculated that daidzein had the ability to induce osteoclasts directly or indirectly from their progenitors and might be a tool by study osteoclast differentiation.
Tobe
H
H
Pharma Research and Development Division, Hoechst Japan Limited, Saitama, Japan.
Komiyama
O
O
Komiyama
Y
Y
Maruyama
H B
HB
D000818:Animals; D001862:Bone Resorption; D001842:Bone and Bones / Q000187:drug effects*; D002452:Cell Count / Q000187:drug effects; D002478:Cells, Cultured; D004968:Estrogens, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D019833:Genistein; D066298:In Vitro Techniques; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects
D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; C004742:daidzein; C018986:ipriflavone; D019833:Genistein