title: A study of Escherichia coli adenylosuccinate synthetase association states and the interface residues of the homodimer.
abstract: The state of aggregation of adenylosuccinate synthetase from Escherichia coli is a point of controversy, with crystal structures indicating a dimer and some solution studies indicating a monomer. Crystal structures implicate Arg143 and Asp231 in stabilizing the dimer, with Arg143 interacting directly with bound IMP of the 2-fold related subunit. Residue Arg143 was changed to Lys and Leu, and residue Asp231 was changed to Ala. Matrix-assisted laser desorption ionization mass spectroscopy and analytical ultracentrifugation of the wild-type and the mutant enzymes indicate a mixture of monomers and dimers, with a majority of the enzyme in the monomeric state. In the presence of active site ligands, the wild-type enzyme exists almost exclusively as a dimer, whereas the mutant enzymes show only slightly decreased dissociation constants for the dimerization. Initial rate kinetic studies of the wild-type and mutant enzymes show similar kcat and Km values for aspartate. However, increases in the Km values of GTP and IMP are observed for the mutant. Changes in dissociation constants for IMP are comparable with changes in Km values. Our results suggest that IMP binding induces enzyme dimerization and that two residues in the interface region, Arg143 and Asp231, play significant roles in IMP and GTP binding.
authors.affiliation: Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Gorrell
authors.forename: R B
authors.initials: RB
authors.lastname: Honzatko
authors.forename: H J
authors.initials: HJ
authors.lastname: Fromm
mesh_terms: D000265:Adenylosuccinate Synthase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation
chemical_list: D000265:Adenylosuccinate Synthase