Cloning of human and mouse cDNAs encoding novel zinc finger proteins expressed in cerebellum and hippocampus.
We identified a novel gene, kf-1, highly expressed in the normal cerebellum but not in the cerebral cortex, the expression of which could have been augmented in the cerebral cortex of a sporadic Alzheimer's disease patient. We cloned human and mouse entire kf-1 cDNAs encoding conserved 79 kDa proteins containing a zinc-binding RING-H2 finger motif at the carboxy-terminus as found in acetylcholine receptor-associated protein (RAPsyn). The 3'-untranslated regions are highly conserved between human and mouse as to constitute a common mRNA secondary structure. In situ hybridization analysis of mouse brain sections revealed strong kf-1 expression in the cerebellum and hippocampus. We propose that KF-1 is involved in membranous protein-sorting apparatus similarly to RAPsyn. We mapped the human kf-1 gene to 2p11.2.
Yasojima
K
K
Department of Biochemistry and Molecular Genetics, Kyoto Prefectural University of Medicine, Japan.
Tsujimura
A
A
Mizuno
T
T
Shigeyoshi
Y
Y
Inazawa
J
J
Kikuno
R
R
Kuma
K
K
Ohkubo
K
K
Hosokawa
Y
Y
Ibata
Y
Y
Abe
T
T
Miyata
T
T
Matsubara
K
K
Nakajima
K
K
Hashimoto-Gotoh
T
T
D000544:Alzheimer Disease / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002531:Cerebellum / Q000378:metabolism*; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C105129:RNF103 protein, human; D044767:Ubiquitin-Protein Ligases