title: Use of antibodies against the human antigen of erythroblasts for the detection of nucleated erythrocytes in the maternal circulation.
abstract: In the present study, we tried to detect nucleated erythrocytes in maternal blood by means of the HAE9 antibody that binds to the antigen of erythroblasts (Ag-Eb) on nucleated erythroid cells. Umbilical cord blood and placental cells, which are known to contain a high number of nucleated erythrocytes, indeed demonstrated a high percentage of Ag-Eb-positive cells. In comparison, HAE9 stained up to 4.5% of the peripheral blood cells collected from pregnant women, but not those of nonpregnant women or normal men. These results, demonstrating a simple and reliable method for the identification of nucleated erythrocytes in the maternal circulation using the anti-Ag-Eb antibody, might open new possibilities for the detection of nucleated erythrocytes in maternal blood.
authors.affiliation: Department of Embryology and Teratology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Savion
authors.forename: H
authors.initials: H
authors.lastname: Carp
authors.forename: J
authors.initials: J
authors.lastname: Shepshelovich
authors.forename: J
authors.initials: J
authors.lastname: Irlin
authors.forename: M
authors.initials: M
authors.lastname: Kostykov
authors.forename: A
authors.initials: A
authors.lastname: Fein
authors.forename: V
authors.initials: V
authors.lastname: Toder
mesh_terms: D000911:Antibodies, Monoclonal; D000941:Antigens / Q000097:blood; D002467:Cell Nucleus; D004900:Erythroblasts / Q000276:immunology*; D004912:Erythrocytes / Q000648:ultrastructure*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D011247:Pregnancy
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens