Elimination of blood-derived macrophages inhibits the release of interleukin-1 and the entry of leukocytes into the cerebrospinal fluid in experimental pneumococcal meningitis.
Parameters of inflammation during pneumococcal meningitis were determined in rabbits after monocyte elimination by dichloromethylene diphosphonate (Cl(2)MDP)-containing mannosylated liposomes in comparison with untreated controls. Monocyte depletion reduced the migration of white blood cells into the cerebrospinal fluid (CSF) (medians: 42 versus 2146/mm3 at 18 h, 323 versus 7413/mm3 at 24 h p.i., p < 0.01). CSF IL-1beta concentrations were lower in depleted animals (379 versus 3282 pg/ml, 24 h p.i., p < 0.01), whereas TNF-alpha concentrations were not different. Monocyte-depleted animals lost body temperature during the experiment carried out in anaesthesia (p = 0.01) indicating that macrophages are necessary for thermogenesis during meningitis.
Zysk
G
G
Department of Neurology, University of Gottingen, Germany.
Brück
W
W
Huitinga
I
I
Fischer
F R
FR
Flachsbarth
F
F
van Rooijen
N
N
Nau
R
R
D000818:Animals; D001773:Blood Cells / Q000187:drug effects / Q000502:physiology*; D001831:Body Temperature; D002465:Cell Movement; D002555:Cerebrospinal Fluid / Q000166:cytology*; D004002:Clodronic Acid / Q000494:pharmacology; D007375:Interleukin-1 / Q000378:metabolism*; D007962:Leukocytes / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008586:Meningitis, Pneumococcal / Q000134:cerebrospinal fluid* / Q000473:pathology; D011817:Rabbits; D013997:Time Factors
D007375:Interleukin-1; D004002:Clodronic Acid