title: Epitope mapping of a monoclonal antibody specific to feline panleukopenia virus and mink enteritis virus.
abstract: To obtain monoclonal antibodies (MAbs) specific to feline panleukopenia virus (FPLV) and mink enteritis virus (MEV), 15 hybridomas secreting MAbs against MEV-Abashiri were established and the properties of the MAbs were analyzed. The cross-reactivity of MAbs revealed that one MAb, P2-215 was specific for FPLV and MEV, whereas the remaining fourteen MAbs reacted with canine parvovirus (CPV), FPLV, and MEV. Epitope analyses using various CPV/MEV chimeric viruses revealed that the MAb P2-215 recognized the epitope comprised of amino acid 93-Lys in VP2, which is known to be FPLV and MEV-specific.
authors.affiliation: Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Horiuchi
authors.forename: M
authors.initials: M
authors.lastname: Mochizuki
authors.forename: N
authors.initials: N
authors.lastname: Ishiguro
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: M
authors.initials: M
authors.lastname: Shinagawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D002415:Cats; D003429:Cross Reactions; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D028301:Feline Panleukopenia Virus / Q000276:immunology*; D006825:Hybridomas; D008907:Mink
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes