In vivo and in vitro clomipramine treatment decreases the migration of macrophages in the rat.
We studied the effect of in vitro and in vivo treatment with the tricyclic antidepressant clomipramine on spontaneous mobility and on N-formyl-L-methionil-L-leucyl-L-phenylalanine (fMLP)-induced chemotaxis of rat peritoneal macrophages. When added in vitro clomipramine was able to diminish (from 10(-4) to 10(-7) M) both spontaneous and stimulated migration of macrophages. A similar effect was observed after the in vivo administration of the drug. In fact, both spontaneous and fMLP-induced mobility of peritoneal macrophages in vitro were significantly reduced after the subcutaneous injection of 20 and 40 mg/kg of clomipramine in comparison to the chemotaxis of macrophages obtained from saline-treated animals. These results give further evidence that psychoactive drugs can affect some immune parameters, and could contribute to explain the antiinflammatory action of clomipramine.
Sacerdote
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Department of Pharmacology, University of Milan, Italy. sacerdot@imiucca.csi.uniml.it
Bianchi
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Panerai
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D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000008:administration & dosage / Q000494:pharmacology*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002997:Clomipramine / Q000008:administration & dosage / Q000494:pharmacology*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D007279:Injections, Subcutaneous; D008264:Macrophages / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000929:Antidepressive Agents, Tricyclic; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002997:Clomipramine