title: Cloning of cDNA encoding the rice 22 kDa protein of Photosystem II (PSII-S) and analysis of light-induced expression of the gene.
abstract: Cloning of rice cDNA encoding the chlorophyll-binding 22 kDa protein of Photosystem II (PSII-S) and the light-induced expression of the gene are reported. One of the light-responsive cDNA clones, isolated by screening with a light-specific subtracted cDNA probe, was shown to encode PSII-S of rice. Genomic Southern analysis suggested that the PSII-S gene, psbS, is a single-copy gene in rice. A brief exposure to red light induced a severalfold increase in the steady state level of PSII-S transcripts in etiolated seedlings. The red light effect was reversed by far-red light, suggesting involvement of phytochrome in the PSII-S gene regulation. Prolonged exposure (3 h) to blue light, however, revealed a much stronger effect than red light on the accumulation of PSII-S transcripts in the etiolated seedlings. In dark-adapted green plants, prolonged exposure to blue light induced re-accumulation of transcripts encoding PSII-S, whereas red light had little effect.
authors.affiliation: National Institute of Agrobiological Resources, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: E
authors.initials: E
authors.lastname: Harada
authors.forename: M
authors.initials: M
authors.lastname: Kasai
authors.forename: K
authors.initials: K
authors.lastname: Shoji
authors.forename: M
authors.initials: M
authors.lastname: Miyao
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D003624:Darkness; D018506:Gene Expression Regulation, Plant; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D012639:Seeds / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins