title: A high-performance liquid chromatography analysis of plasma artemisinin using a glassy carbon electrode for reductive electrochemical detection.
abstract: A high-performance liquid chromatography assay equipped with a glassy carbon electrode for electrochemical detection (HPLC-ECD) was developed at reductive mode for the analysis of artemisinin, the antimalarial drug from Artemisia annua (Asteraceae) in human plasma. This method was selective, sensitive, and produced satisfactory recovery, precision, and accuracy. Analysis of plasma samples from 8 male volunteers given 10 mg kg-1 of artemisinin orally as an aqueous suspension showed a mean peak plasma concentration (Cmax) of 580.89 ng ml-1 +/- 88.64 SD at 2.5 h +/- 0.5 SD after dosing, and the mean area under the plasma concentration-time curve (AUC0-infinity) was 2227.57 ng h ml-1 +/- 677.22 SD. In addition, the elimination rate constant (Ke), elimination half-life (t1/2), and apparent volume of distribution (Vd) were calculated to be 0.2971 h-1 +/- 0.0644 SD, 2.42 h +/- 0.46 SD, and 16.26 l kg-1 +/- 3.44 SD, respectively.
authors.affiliation: School of Pharmaceutical Science, University of Science Malaysia, Penang, Malaysia.
authors.forename: K L
authors.initials: KL
authors.lastname: Chan
authors.forename: K H
authors.initials: KH
authors.lastname: Yuen
authors.forename: S
authors.initials: S
authors.lastname: Jinadasa
authors.forename: K K
authors.initials: KK
authors.lastname: Peh
authors.forename: W T
authors.initials: WT
authors.lastname: Toh
mesh_terms: D000328:Adult; D000962:Antimalarials / Q000097:blood* / Q000493:pharmacokinetics; D037621:Artemisinins; D002244:Carbon; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D000962:Antimalarials; D037621:Artemisinins; D012717:Sesquiterpenes; D002244:Carbon; C031327:artemisinin