title: Effects of 3,4-methylenedioxymethamphetamine in decomposing tissues on the development of Parasarcophaga ruficornis (Diptera:Sarcophagidae) and detection of the drug in postmortem blood, liver tissue, larvae, and puparia.
abstract: Larvae of Parasarcophaga ruficornis (Fabricius) (Diptera: Sarcophagidae) were reared on tissues from rabbits administered different dosages of 3,4-methylenedioxymethamphetamine to study the effects of this drug on the development of this insect species. The rabbits were given 11, 22.5, and 67 mg of the drug via ear vein infusion. These dosages correspond to a 0.5, 1.0, and 2.0 median lethal dose. Larvae from colonies fed on tissues from the rabbit receiving 67 mg and the control developed more rapidly from hours 24 through 114. The period required for completion of larval development was significantly shorter for the larvae from the colony fed on liver tissues from the rabbit receiving the 67 mg dosage. This colony also had the lowest total mortality rate during development. No significant differences were observed among the colonies in the duration of the puparial period. Analyses by liquid chromatography/mass spectrometry of the larvae and empty puparial cases following base extraction detected the drug in quantities directly related to the dosage of the drug administered to the rabbits serving as a food source. These results have implications for the determination of time since death when gauged by the development of this insect species.
authors.forename: M L
authors.initials: ML
authors.lastname: Goff
authors.forename: M L
authors.initials: ML
authors.lastname: Miller
authors.forename: J D
authors.initials: JD
authors.lastname: Paulson
authors.forename: W D
authors.initials: WD
authors.lastname: Lord
authors.forename: E
authors.initials: E
authors.lastname: Richards
authors.forename: A I
authors.initials: AI
authors.lastname: Omori
mesh_terms: D000818:Animals; D004175:Diptera / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development*; D013287:Illicit Drugs / Q000097:blood* / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D008099:Liver / Q000737:chemistry / Q000187:drug effects*; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000097:blood* / Q000494:pharmacology*; D011180:Postmortem Changes; D011679:Pupa / Q000187:drug effects; D011817:Rabbits
chemical_list: D013287:Illicit Drugs; D018817:N-Methyl-3,4-methylenedioxyamphetamine