Lipid peroxidation in ethylene glycol induced hyperoxaluria and calcium oxalate nephrolithiasis.
OBJECTIVE
To determine if lipid peroxidation plays a role in renal injury associated with experimental nephrolithiasis.


METHODS
Hyperoxaluria was produced in rats by ethylene glycol in drinking water. At 15, 30 and 60 days of treatment, urinary oxalate, lipid peroxide, calcium oxalate crystals, enzymes and tissue lipid peroxide were measured.


RESULTS
Urinary oxalate increased significantly at all time periods and was associated with crystalluria. Lipid peroxides in kidney tissue and urine increased at all time periods. Tissue calcium oxalate crystal deposits from 0 to 1+ were present on day 15, but present in all animals on days 30 and 60. Renal tubular cell damage was confirmed by an increase in urinary marker enzymes.


CONCLUSIONS
Renal cell damage is associated with lipid peroxide production indicating cell injury due to the production of free radicals. The damage appears due primarily to hyperoxaluria and is augmented by crystal deposition in the renal tubules.
Thamilselvan
S
S
Department of Pathology, College of Medicine, University of Florida, Gainesville 32610, USA.
Hackett
R L
RL
Khan
S R
SR
D000818:Animals; D002129:Calcium Oxalate / Q000652:urine; D019855:Ethylene Glycol; D005026:Ethylene Glycols; D006959:Hyperoxaluria / Q000139:chemically induced / Q000378:metabolism*; D007669:Kidney Calculi / Q000209:etiology* / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D005026:Ethylene Glycols; D002129:Calcium Oxalate; D019855:Ethylene Glycol