Survey of sinonasal inverted papillomata for Epstein-Barr virus.
BACKGROUND
Several studies have indicated an etiologic role for viruses in the development of sinonasal inverted papillomata (IP). A recent report demonstrates a strong relationship (65%) between Epstein-Barr virus (EBV) and these lesions using polymerase chain reaction (PCR) analysis.


METHODS
The present study analyzes a series of paraffin-embedded tissues, comprising 25 surgically resected IPs and four fungiform papillomata (FP) for the presence of EBV using a sensitive in situ hybridization (ISH) assay and PCR.


RESULTS
None of the specimens examined showed evidence of EBV infection by ISH, and only two papillomata (one sinonasal IP and one FP) gave positive reactions for EBV using PCR.


CONCLUSIONS
These data challenge the previous report and suggest that EBV is not a significant etiopathologic factor to be considered in the development of sinonasal IP.
Dunn
S T
ST
Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
Clark
G D
GD
Cannon
T C
TC
Min
K W
KW
D000368:Aged; D001483:Base Sequence; D015139:Blotting, Southern; D046508:Culture Techniques; D006566:Herpesviridae Infections / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D015994:Incidence; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009669:Nose Neoplasms / Q000175:diagnosis / Q000209:etiology / Q000821:virology*; D010212:Papilloma / Q000175:diagnosis / Q000209:etiology / Q000821:virology*; D010256:Paranasal Sinuses / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology