Interaction of HPC-1/syntaxin 1A with the cytoskeletal protein, tubulin.
It is believed that HPC-1/syntaxin 1A regulates the intracellular membrane transport. We found a possible tubulin binding motif like sequence in residues 89 approximately 106 of HPC-1/syntaxin 1A. To determine whether or not HPC-1/syntaxin 1A binds to tubulin, we performed in vitro binding studies. We showed that both the rat brain and recombinant HPC-1/syntaxin 1A bound to tubulin in vitro. Competition experiments with synthetic peptides revealed that HPC-1/syntaxin 1A bound to tubulin at residues 89 approximately 106 which were supposed to constitute the tubulin binding consensus sequence.
Fujiwara
T
T
Department of Physiology, Kyorin University School of Medicine, Tokyo, Japan.
Yamamori
T
T
Yamaguchi
K
K
Akagawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D050827:Syntaxin 1; D014404:Tubulin / Q000378:metabolism*
D000954:Antigens, Surface; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C494213:Stx1a protein, rat; D050827:Syntaxin 1; D014404:Tubulin