title: Establishing a model to study the regulation of the lactose operon in Lactobacillus casei.
abstract: The chromosomally encoded lactose-specific phosphoenol pyruvate-dependent phosphotransferase system (PTS) has been investigated in Lactobacillus casei ATCC 393 [pLZ15-] and it was considered an excellent system to study the regulation of the lactose operon. This chromosomal operon has been cloned and sequenced, being 99% homologous to that encoded on the plasmid pLZ64. Expression of the lactose operon in different mutants of L. casei ATCC 393 [pLZ15-] and primer extension analysis revealed that it is subject to a dual regulation: (i) glucose repression possibly mediated by CcpA and PTS elements, and (ii) induction by lactose through transcriptional antitermination.
authors.affiliation: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Polígono de la Coma sln, Burjasot, Valencia, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Gosalbes
authors.forename: V
authors.initials: V
authors.lastname: Monedero
authors.forename: C A
authors.initials: CA
authors.lastname: Alpert
authors.forename: G
authors.initials: G
authors.lastname: Pérez-Martínez
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005798:Genes, Bacterial / Q000502:physiology; D017930:Genes, Reporter; D007763:Lac Operon / Q000502:physiology*; D007780:Lacticaseibacillus casei / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics*; D001616:beta-Galactosidase
chemical_list: D001616:beta-Galactosidase