Antibodies to macrophage inflammatory protein-1beta in preoptic area of rats fail to suppress PGE2 hyperthermia.
This study determined whether macrophage inflammatory protein-1beta (MIP-1beta) plays a role in the hyperthermia caused by prostaglandin E2 (PGE2) given intracerebroventricularly (i.c.v.) in the rat. In these experiments, anti-murine MIP-1beta antibody (anti-MIP-1beta) was micro-injected in the anterior hypothalamic, preoptic area (AH/POA) just before i.c.v. PGE2. The results showed that anti-MIP-1beta failed to alter the PGE2 hyperthermia. However, immunocytochemical studies revealed MIP-1beta immunoreactivity detectable in both the organum vasculosum laminae terminalis (OVLT) and AH/POA in the febrile rat. These data thus demonstrate that MIP-1beta is sequestered in diencephalic structures underlying thermoregulation even though it is not involved in PGE2 hyperthermia. This dissociation supports the viewpoint that at least two distinct systems exist in the brain which underlie a febrile response: MIP-1beta underlies one component whereas PGE2 comprises the other.
Armengol
J A
JA
Departamento de Ciencias Morfologicas Facultad de Medicina, Universidad de Sevilla, Spain.
Benamar
K
K
Fernández-Alonso
A
A
Sancibrián
M
M
Myers
R D
RD
Miñano
F J
FJ
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000494:pharmacology*; D054407:Chemokine CCL4; D015232:Dinoprostone; D005334:Fever / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D007031:Hypothalamus / Q000378:metabolism; D007032:Hypothalamus, Anterior / Q000378:metabolism; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D019402:Macrophage Inflammatory Proteins / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008845:Microinjections; D011301:Preoptic Area / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D015232:Dinoprostone