Reciprocal gene expression of rat fibroglycan and beta-actin during the course of regeneration after D-galactosamine liver injury.
OBJECTIVE
Fibroglycan (FG) is a major heparan sulfate proteoglycan (HSPG) in the rat liver that is mainly distributed on the surface of hepatocytes. HSPG may play some important roles in the regeneration of liver by interacting with various growth factors such as bFGF and HB-EGF. However, little is known about the function of FG. We reported that after injury caused by D-galactosamine, regeneration started on the following day and peaked on day 2. To clarify the function of FG in liver regeneration, we investigated the gene expression of FG during regeneration after D-galactosamine injury.


METHODS
Rats were given D-galactosamine on day 0. Liver RNA was collected from day 0 to day 7. The gene expression of FG and beta-actin (as a representative cytoskeleton) was examined by Northern and/or Slot blotting.


RESULTS
FG gene expression was markedly decreased on day 2, but totally recovered on day 3. In contrast, beta-actin gene expression was markedly increased on day 2 and returned to the normal level on day 3. Expression of the FG and beta-actin genes was reciprocal.


CONCLUSIONS
FG expression is transiently suppressed when cytoskeleton gene expression is enhanced at the early phase of liver regeneration.
Shinji
T
T
First Department of Internal Medicine, Okayama University Medical School.
Koide
N
N
Hada
H
H
Sasaki
S
S
Oka
T
T
Takayama
N
N
Shiraha
S
S
Ujike
K
K
Nakamura
M
M
Hanafusa
T
T
Yumoto
Y
Y
Tsuji
T
T
D000199:Actins / Q000235:genetics*; D000818:Animals; D005688:Galactosamine; D015870:Gene Expression; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D009336:Necrosis; D011509:Proteoglycans / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D053669:Syndecan-2
D000199:Actins; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D012333:RNA, Messenger; C508247:Sdc2 protein, rat; D053669:Syndecan-2; D005688:Galactosamine