Changes in protein composition during chick embryo retina development.
Two dimensional gel electrophoresis was employed to analyse the protein composition of chick embryo retina during "in ovo" development in order to individuate some components which are typical of different stages of growth. The study of protein composition of 7-day-old chick embryo retinas revealed the presence of about eighty different soluble components. In four of these proteins the staining intensity decreased during retina development. One of these components, of 35 KD with an isoelectric point of about 5.15, was partially purified by means of preparative isoelectric focusing. Other components, which were absent at day 7 appeared during the second week of development and were clearly visualised at day 16. These results were also compared with those obtained by means of 2DGE performed on liver and brain of chick embryo at day 7 and 16 of development.
Carabillò
M
M
Istituto di Chimica Biologica, Università of Palermo, Policlinico, Italy.
Gerbino
E
E
Main
H
H
Calvaruso
G
G
Tesoriere
G
G
D000367:Age Factors; D000818:Animals; D002642:Chick Embryo; D004527:Egg Proteins / Q000302:isolation & purification / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007525:Isoelectric Focusing; D012160:Retina / Q000737:chemistry / Q000196:embryology*; D013997:Time Factors
D004527:Egg Proteins; D005136:Eye Proteins