Atomic force microscope imaging of chromosome structure during G-banding treatments.
Surface topography of human chromosomes was examined by atomic force microscopy during treatments for G-banding. Trypsin treatment resulted in a structural modification in the chromatin. Subsequent Giemsa staining caused a general swelling of the chromosomal surface that was greater in the areas of G-band positive regions. By means of a quantitative evaluation method we showed that the G-banding process produces a 10-fold enhancement of a pre-existing pattern of chromatin between G-band positive and G-band negative regions on mitotic chromosomes.
Musio
A
A
Dipartimento di Scienze dell' Ambiente e del Territorio, Università di Pisa, Italy.
Mariani
T
T
Frediani
C
C
Ascoli
C
C
Sbrana
I
I
D001399:Azure Stains; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D006801:Humans; D018625:Microscopy, Atomic Force; D014357:Trypsin / Q000378:metabolism
D001399:Azure Stains; D014357:Trypsin