Cartilage markers in synovial fluid in symptomatic knee osteoarthritis.
OBJECTIVE
To investigate if the relative content of aggrecan and cartilage oligomeric matrix protein (COMP) in synovial fluid lavage samples differs between individuals with knee pain with or without evidence of radiological knee osteoarthritis (joint space narrowing).


METHODS
In a community based cohort of 204 individuals aged 35-54 years with chronic (> 3 months duration) knee pain, a subgroup of 45 subjects with radiographic osteoarthritis, grade I according to the Ahlbäck classification, was randomly chosen; 45 individuals, age and sex matched from the same cohort with chronic knee pain but with normal radiographs, served as controls, Knee joint fluid was obtained by a standardised lavage procedure. The concentrations of aggrecan core protein epitopes and a cartilage matrix protein (COMP) were determined by immunoassays and the aggrecan/COMP concentration ratio was calculated.


RESULTS
The aggrecan/COMP ratio was higher (P < 0.001) in the group with radiographic osteoarthritis than in the control group.


CONCLUSIONS
The higher aggrecan/COMP ratios in osteoarthritis could reflect increased cartilage matrix turnover in osteoarthritis with predominant release of aggrecan fragments. Synovial fluid analysis of cartilage markers holds promise as a useful means of monitoring changes in the cartilage turnover in studies of pathogenic mechanisms in osteoarthritis.
Petersson
I F
IF
Department of Rheumatology, Lund University, Sweden.
Sandqvist
L
L
Svensson
B
B
Saxne
T
T
D000328:Adult; D053674:Aggrecans; D015415:Biomarkers / Q000032:analysis; D064236:Cartilage Oligomeric Matrix Protein; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D015331:Cohort Studies; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007719:Knee Joint / Q000000981:diagnostic imaging; D037181:Lectins, C-Type; D008297:Male; D064235:Matrilin Proteins; D008875:Middle Aged; D010003:Osteoarthritis / Q000000981:diagnostic imaging / Q000378:metabolism*; D010146:Pain / Q000378:metabolism; D011509:Proteoglycans / Q000032:analysis*; D011859:Radiography; D013582:Synovial Fluid / Q000737:chemistry*
D053674:Aggrecans; D015415:Biomarkers; D064236:Cartilage Oligomeric Matrix Protein; D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D064235:Matrilin Proteins; D011509:Proteoglycans; C577553:TSP5 protein, human