title: Immunohistochemical characterization of monoclonal antibodies (PDs) as markers of the periderm in the developing chicken embryo.
abstract: The outermost surface cell layer of the developing embryo, the periderm, arises from the initial single layer of ectoderm and is eventually exfoliated from the stratified epidermis, which has the same ectodermal origin. In this study, monoclonal antibodies against chicken limb bud ectoderm were generated and screened for those which stained the periderm. Four separate antibodies termed PD2, 3, 7 and 9 were obtained from 180 mixed hybridomas. These PD antibodies stained the periderm selectively at all stages examined (stage 20-42). By correlating the results of immunohistochemistry with observations made by transmission electron microscopy, it was revealed that PD antibodies stained both the squamous periderm at an early stage and rounded bulging peridermal cells just before exfoliation. Therefore we feel that PD antibodies may be useful in further systematic investigations of the development and function of the chicken embryonic periderm.
authors.affiliation: Division of Electron Microscopy, Dental Research Center, Nihon University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Isokawa
authors.forename: J
authors.initials: J
authors.lastname: Honma
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Aoki
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Yokoyama
authors.forename: S
authors.initials: S
authors.lastname: Endo
authors.forename: Y
authors.initials: Y
authors.lastname: Toda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology*; D002642:Chick Embryo; D004475:Ectoderm / Q000276:immunology* / Q000648:ultrastructure; D004817:Epidermis / Q000196:embryology* / Q000276:immunology; D004848:Epithelium / Q000196:embryology / Q000276:immunology; D018878:Limb Buds / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation