Benzophenone photoprobes for phosphoinositides, peptides and drugs.
Benzophenones (BP) and related aryl ketone photophores have become established as the photoactivatable group of choice for high-efficiency covalent modification of hydrophobic regions of binding proteins, including enzymes and receptors that recognize peptide hormones, (oligo) nucleotides and nucleosides, phosphoinositides, inositol polyphosphates and a wide variety of therapeutic molecules. This review presents the advantages of BP as photoaffinity labels and provides specific examples from the last 3 years of applications of BP-containing ligands used in biochemistry.
Prestwich
G D
GD
Department of Chemistry, University at Stony Brook, NY, USA. gprestwich@deans.pharm.utah.edu
Dormán
G
G
Elliott
J T
JT
Marecak
D M
DM
Chaudhary
A
A
D000345:Affinity Labels / Q000138:chemical synthesis / Q000737:chemistry*; D001577:Benzophenones / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010716:Phosphatidylinositols / Q000032:analysis*; D018462:Photobiology / Q000379:methods*; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry*
D000345:Affinity Labels; D001577:Benzophenones; D010455:Peptides; D004364:Pharmaceutical Preparations; D010716:Phosphatidylinositols; D017319:Photosensitizing Agents; C047723:benzophenone