title: Augmented expression of the endothelin-A receptor gene in cultured mesangial cells from stroke-prone spontaneously hypertensive rats.
abstract: 1. To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET-A receptor (ET-AR) gene and the ET-B receptor (ET-BR) gene in cultured mesangial cells isolated from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET-AR predominantly. The level of the ET-AR mRNA in mesangial cells from SHRSP was 5-fold higher than that in the cells from WKY. The ET-BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET-AR gene was markedly augmented in mesangial cells from SHRSP.
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Hiraoka
authors.forename: H
authors.initials: H
authors.lastname: Arai
authors.forename: T
authors.initials: T
authors.lastname: Yoshimasa
authors.forename: K
authors.initials: K
authors.lastname: Takaya
authors.forename: Y
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: J
authors.initials: J
authors.lastname: Yamashita
authors.forename: S
authors.initials: S
authors.lastname: Suga
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
authors.forename: G
authors.initials: G
authors.lastname: Shirakami
authors.forename: H
authors.initials: H
authors.lastname: Itoh
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D002561:Cerebrovascular Disorders / Q000235:genetics / Q000503:physiopathology; D005920:Glomerular Mesangium / Q000378:metabolism*; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017466:Receptors, Endothelin / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D017466:Receptors, Endothelin