title: Structure characterization of the central repetitive domain of high molecular weight gluten proteins. II. Characterization in solution and in the dry state.
abstract: The structure of the central repetitive domain of high molecular weight HMW) wheat gluten proteins was characterized in solution and in the dry state using HMW proteins Bx6 and Bx7 and a subcloned, bacterially expressed part of the repetitive domain of HMW Dx5. Model studies of the HMW consensus peptides PGQGQQ and GYYPTSPQQ formed the basis for the data analysis (van Dijk AA et al., 1997, Protein Sci 6:637-648). In solution, the repetitive domain contained a continuous nonoverlapping series of both type I and type II II beta-turns at positions predicted from the model studies; type II beta-turns occurred at QPGQ and QQGY sequences and type I beta-turns at YPTS and SPQQ. The subcloned part of the HMW Dx5 repetitive domain sometimes migrated as two bands on SDS-PAGE; we present evidence that this may be caused by a single amino acid insertion that disturbs the regular structure of beta-turns. The type I beta-turns are lost when the protein is dried on a solid surface, probably by conversion to type II beta-turns. The homogeneous type II beta-turn distribution is compatible with the formation of a beta-spiral structure, which provides the protein with elastic properties. The beta-turns and thus the beta-spiral are stabilized by hydrogen bonds within and between turns. Reformation of this hydrogen bonding network after, e.g., mechanical disruption may be important for the elastic properties of gluten proteins.
authors.affiliation: Department of Biochemistry and Biophysical Chemistry, University of Groningen, The Netherlands.
authors.forename: A A
authors.initials: AA
authors.lastname: Van Dijk
authors.forename: E
authors.initials: E
authors.lastname: De Boef
authors.forename: A
authors.initials: A
authors.lastname: Bekkers
authors.forename: L L
authors.initials: LL
authors.lastname: Van Wijk
authors.forename: E
authors.initials: E
authors.lastname: Van Swieten
authors.forename: R J
authors.initials: RJ
authors.lastname: Hamer
authors.forename: G T
authors.initials: GT
authors.lastname: Robillard
mesh_terms: D000595:Amino Acid Sequence; D002847:Chromatography, Agarose; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011994:Recombinant Proteins; D005983:Glutens