2-phenylethylamine catabolism by Escherichia coli K-12: gene organization and expression.
A gene encoding phenylacetaldehyde dehydrogenase (PAD), the enzyme involved together with a copper-topaquinone-containing amine oxidase in the initial steps of 2-phenylethylamine catabolism, was located at 31.1 min on the Escherichia coli K-12 genetic map. It was immediately adjacent to the gene encoding the amine oxidase but transcribed in the opposite direction. The purified PAD acted almost equally well on phenylacetaldehyde, 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde. It had a subunit size of 54 kDa and its deduced amino acid sequence was approximately 40% identical to various eukaryotic and prokaryotic aldehyde dehydrogenases. A third gene encoding a positive regulatory protein required for expression of the amine oxidase and PAD genes was located next to the PAD gene. A gene previously located in this position was reported to encode a second amine oxidase but this was not confirmed. The nucleotide sequence from 1447 to 1450 kb on the E. coli K-12 physical map has been determined.
Hanlon
Steven P
SP
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK.
Hill
Terena K
TK
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK.
Flavell
Margaret A
MA
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK.
Stringfellow
Joseph M
JM
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK.
Cooper
Ronald A
RA
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK.
D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing); D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D010627:Phenethylamines / Q000378:metabolism*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013379:Substrate Specificity; D014158:Transcription, Genetic
D029968:Escherichia coli Proteins; D010627:Phenethylamines; C029261:phenethylamine; D000445:Aldehyde Oxidoreductases; C053855:phenylacetaldehyde dehydrogenase; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors