The studies of HLA class II (HLA-DR) system by PCR-SSO typing and the relationship with serological method.
By using two methods together, namely, the conventional serological typing and the new method which is oligonucleotide typing, in order to study the HLA system, it was concluded that the new method was better. Some subtypes were detectable from the new method, whereas, the serological typing yielded negative results such as DRB1 subtype "0103". This method, therefore, provided more information in order to study the HLA system. However, the new method was twice as expensive as the conventional serological method.
Charoenwongse
P
P
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Kangwanshiratada
O
O
Chatsuwan
T
T
Boonnam
R
R
Tumwasorn
S
S
Bhattarakosol
P
P
D000328:Adult; D001788:Blood Grouping and Crossmatching; D003429:Cross Reactions; D000939:Epitopes; D005260:Female; D006684:HLA-DR Antigens / Q000145:classification* / Q000235:genetics; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods
D000939:Epitopes; D006684:HLA-DR Antigens; D015345:Oligonucleotide Probes