title: Developmental regulation of tissue transglutaminase during human placentation and expression in neoplastic trophoblast.
abstract: The expression of tissue transglutaminase (tTG) was studied during the formation of the normal human placenta and in molar pregnancies and choriocarcinoma, in order to correlate its expression with the functional characteristics of the recognized trophoblast cell types. tTG expression was found to be developmentally regulated. Before 6-7 weeks' gestation, only the chorionic villous cytotrophoblast expresses tTG. Thereafter the overlying syncytiotrophoblast becomes positive. tTG expression is gradually downregulated in the intermediate trophoblast cells emerging from the tips of the chorionic villi invading the uterine tissue. In the decidual wall, the intermediate trophoblast does not express tTG, whereas scattered syncytial cells, the placental bed giant cells, express tTG. Villi from complete hydatidiform mole (CHM) show tTG expression in both the cyto- and the syncytiotrophoblast. The intermediate trophoblast cells from CHM show heterogeneous tTG expression, with a majority of negative cells, whereas extravillous syncytia always express tTG. In choriocarcinoma, the tumour cells show heterogeneous tTG expression, with a majority of positive cells. Analysis of tTG protein and mRNA in placental extracts by Western and Northern blotting did not provide evidence for expression of the truncated form of tTG found in some cell types. The regulated expression of tTG in the normal placenta suggests that the enzyme is involved in important trophoblastic functions and may participate in the control of invasion.
authors.affiliation: Department of Medical Biochemistry, University of Aarhus, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Hager
authors.forename: J
authors.initials: J
authors.lastname: Gliemann
authors.forename: S
authors.initials: S
authors.lastname: Hamilton-Dutoit
authors.forename: P
authors.initials: P
authors.lastname: Ebbesen
authors.forename: U
authors.initials: U
authors.lastname: Koppelhus
authors.forename: P H
authors.initials: PH
authors.lastname: Jensen
mesh_terms: D015152:Blotting, Northern; D002822:Choriocarcinoma / Q000201:enzymology; D002824:Chorionic Villi / Q000201:enzymology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D006828:Hydatidiform Mole / Q000201:enzymology; D007124:Immunoenzyme Techniques; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011503:Transglutaminases / Q000378:metabolism*; D014327:Trophoblasts / Q000201:enzymology*; D014594:Uterine Neoplasms / Q000201:enzymology*
chemical_list: D011503:Transglutaminases