[Tissue engineering of human cartilage tissue for reconstructive surgery using biocompatible resorbable fibrin gel and polymer carriers].
Current practical approaches in cartilage engineering still face problems with three dimensional cell distribution or require components for cell immobilization, raising biocompatibility problems. In this study, we present a new model using cells cross-linked by fibrin within biocompatible resorbable polymers. Both components have been in clinical use for a long time. Immunohistochemical procedures showed that this model provides optimal requirements for in vitro cartilage production. Immunochemically, cartilage-specific extracellular components such as proteoglycan, chondroitin sulfate and collagen II were characterized. Histomorphological methods showed a mechanically stable tissue compound that lasted for at least 5 weeks. This model may be the first to provide all biocompatible requirements for in vitro production of autologous cartilage transplants for reconstructive surgery.
Haisch
A
A
HNO-Klinik und Poliklinik, Universitätsklinikum Charité, Medizinische Fakultät der Humboldt-Universität zu Berlin.
Schultz
O
O
Perka
C
C
Jahnke
V
V
Burmester
G R
GR
Sittinger
M
M
D001672:Biocompatible Materials; D002356:Cartilage / Q000166:cytology* / Q000637:transplantation; D002809:Chondroitin Sulfates / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D046508:Culture Techniques; D015718:Fibrin Tissue Adhesive; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D012038:Regeneration / Q000502:physiology*; D012225:Rhinoplasty / Q000379:methods*
D001672:Biocompatible Materials; D015718:Fibrin Tissue Adhesive; D006025:Glycosaminoglycans; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002809:Chondroitin Sulfates; D003094:Collagen
Tissue-engineering humanen Knorpelgewebes für die rekonstruktive Chirurgie unter Verwendung biokompatibler resorbierbarer Fibringel- und Polymervliesstrukturen.