CRF2-4: isolation of cDNA clones encoding the human and mouse proteins.
To search for genes which encode human interferon receptor molecules, we applied the technique of exon trapping to human chromosome 21 yeast artificial chromosomes (YACs). A full length cDNA encoding human CRF2-4 was identified which is shown here to be widely expressed in human tissues. The human CRF2-4 cDNA was used as a probe to screen a mouse macrophage cDNA library. A mouse CRF2-4 cDNA was isolated which encodes a 349-amino-acid (aa) polypeptide that is 69% identical at the aa level with its human counterpart. Mouse CRF2-4 is predicted to be another member of the interferon receptor-class II cytokine receptor family.
Gibbs
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Department of Surgery, San Francisco VA Medical Center, CA 94131, USA. gibbs.verna@sanfrancisco.va.gov
Pennica
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002891:Chromosomes, Human, Pair 21; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D005091:Exons; D005821:Genetic Techniques; D006801:Humans; D053710:Interleukin-10 Receptor beta Subunit; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D018121:Receptors, Cytokine / Q000235:genetics* / Q000378:metabolism*; D017471:Receptors, Interferon / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; C081846:IL10RB protein, human; D053710:Interleukin-10 Receptor beta Subunit; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D017471:Receptors, Interferon