Structural and functional analysis of the 5'-flanking region of the human insulin-like growth factor binding protein (IGFBP)-4 gene.
More than 3 kb of the human (h)IGFBP-4 gene 5'-flanking region was sequenced and assessed for promoter activity. The hIGFBP-4 promoter resides within a CpG island and demonstrates strong basal activity in human osteoblast-like osteosarcoma and COS-7 monkey kidney cells. Transient transfection of cells with hIGFBP-4 promoter-linked deletion constructs demonstrated that multiple cooperating cis-acting elements within 836 bp of the 5'-flanking region contributed to overall promoter strength.
Qin
X
X
J.L. Pettis Memorial Veterans' Medical Center, Department of Medicine, Loma Linda University, CA 92357, USA.
Morales
S
S
Lee
K W
KW
Boonyaratanakornkit
V
V
Baylink
D J
DJ
Mohan
S
S
Strong
D D
DD
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002460:Cell Line; D018899:CpG Islands; D004247:DNA / Q000235:genetics; D006801:Humans; D018974:Insulin-Like Growth Factor Binding Protein 4 / Q000235:genetics*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D014162:Transfection
D018974:Insulin-Like Growth Factor Binding Protein 4; D016376:Oligonucleotides, Antisense; D004247:DNA