Alanylglutamine-enriched total parenteral nutrition improves protein metabolism more than branched chain amino acid-enriched total parenteral nutrition in protracted peritonitis.
Branched chain amino acids (BCAAs) and glutamine are both recommended in catabolic states. The object of this study was to compare the efficacies of alanylglutamine (Ala-Gln)-enriched and BCAA-enriched total parenteral nutrition (TPN) on the protein kinetics in peritonitis. Rats were divided into Ala-Gln and BCAA groups after intraperitoneal implantation of an osmotic pump, delivering a continuous infusion of Escherichia coli. Glutamine composed 30.0% (w/v) of the total amino acids in the Ala-Gln group, and BCAA composed 30.5% (w/v) of the total amino acids in the BCAA group. The two solutions were isocaloric and isonitrogenous. Whole body protein turnover and organ fractional protein synthetic rates (FSR) were measured on days 3 and 5. Serum amino acid levels and mucosal morphology were determined. Ala-Gln group had higher rates of whole body protein turnover, and hepatic FSR on both days. Serum glutamine levels correlated with hepatic and muscle FSR. Ala-Gln TPN group had greater mucosal thickness, numbers of mitoses per crypt, and FSR in distal intestine. Ala-Gln-enriched TPN may be a useful nutritional treatment modality in sepsis.
Naka
S
S
Department of Surgery, The University of Tokyo, Japan.
Saito
H
H
Hashiguchi
Y
Y
Lin
M T
MT
Furukawa
S
S
Inaba
T
T
Fukushima
R
R
Wada
N
N
Muto
T
T
D000596:Amino Acids / Q000097:blood; D000597:Amino Acids, Branched-Chain / Q000627:therapeutic use*; D000818:Animals; D004151:Dipeptides / Q000627:therapeutic use*; D004195:Disease Models, Animal; D008297:Male; D010289:Parenteral Nutrition, Total / Q000379:methods*; D010538:Peritonitis / Q000097:blood / Q000378:metabolism* / Q000628:therapy*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D004151:Dipeptides; D011506:Proteins; C054122:alanylglutamine