Selective depletion of CD8-positive leukocytes does not alter mercuric chloride induced acute renal failure in the rat.
The process of injury and regeneration in different models of acute renal failure is accompanied by the transient interstitial accumulation of mononuclear leukocytes. The relationship between these accumulated cells and the onset and progression of the regeneration process resulting in the complete functional and morphological recovery is still a matter of debate. In this process cell subsets may either be selectively important or combine to a communicative network, signalling the cells at the site of injury. In a first trial to investigate this hypothesis, the CD8-positive subset of leukocytes, consisting mainly of cytotoxic and suppressor T lymphocytes and to a lesser extent of natural killer cells, was depleted in vivo in rats by means of a monoclonal antibody directed against CD8. Although the depletion obtained evidently prevented the infiltration of these cells into the renal interstitium, it could not influence neither the development nor the resolution of renal insufficiency in response to mercuric chloride administration as compared with control animals who had received an irrelevant isotype-matched monoclonal antibody. The extent of renal damage was unaffected as were onset and duration of renal epithelial cell proliferation. Consequently, these data do not support a major role for the CD8-positive cell subset per se in the development of acute nephrotoxic injury and subsequent regeneration.
Ghielli
M
M
Department of Nephrology-Hypertension, University of Antwerp, Belgium.
Verstrepen
W A
WA
Dauwe
S
S
Nouwen
E J
EJ
De Broe
M E
ME
D058186:Acute Kidney Injury / Q000139:chemically induced / Q000276:immunology*; D000818:Animals; D001835:Body Weight; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D003404:Creatinine / Q000097:blood; D004202:Disinfectants; D004848:Epithelium / Q000473:pathology; D005260:Female; D007686:Kidney Tubules, Distal / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000473:pathology; D008627:Mercuric Chloride; D009336:Necrosis; D009395:Nephritis, Interstitial / Q000139:chemically induced / Q000276:immunology*; D011507:Proteinuria; D051381:Rats; D017208:Rats, Wistar
D004202:Disinfectants; D008627:Mercuric Chloride; D003404:Creatinine