title: Effect of temperature on stability of eight components of porcine gallbladder bile.
abstract: Our aim was to evaluate the stability of eight components of porcine bile (pH, total and ionized calcium, total and unconjugated bilirubin, phospholipid, cholesterol, and bile salts) over a 17-day period at three temperatures: -15, 4, and 37 degrees C. The pH and concentrations of total and ionized calcium, phospholipid, cholesterol, and bile salts were stable over 17 days. Total bilirubin was stable at -15 degrees C, but declined over 17 days by approximately 25% at 4 degrees C and 70% at 37 degrees C (P < 0.003). A rapid increase in the unconjugated bilirubin was seen within two days at all temperatures to between 7.5 and 12 times the levels at day 0 (P < 0.009). Thereafter unconjugated bilirubin at -15 and 4 degrees C continued to increase at a much slower rate. By contrast, unconjugated bilirubin at 37 degrees C declined beginning on day 4 and fell to 1.33 times levels at day 0 by day 17 (P < 0.002). We conclude that bile can be stored at -15 degrees C for 17 days with stability of most components, but unconjugated bilirubin will rise. The loss in total bilirubin is significant at 37 degrees C.
authors.affiliation: Department of Medicine, Northwestern University Medical Center, Evanston Hospital, Illinois 60201, USA.
authors.forename: H B
authors.initials: HB
authors.lastname: Chodash
authors.forename: T K
authors.initials: TK
authors.lastname: Tsang
authors.forename: J M
authors.initials: JM
authors.lastname: Pollack
authors.forename: R E
authors.initials: RE
authors.lastname: Eisenman
authors.forename: R M
authors.initials: RM
authors.lastname: Rege
authors.forename: J D
authors.initials: JD
authors.lastname: Ostrow
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry*; D001647:Bile Acids and Salts / Q000032:analysis; D001663:Bilirubin / Q000032:analysis; D002118:Calcium / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010743:Phospholipids / Q000032:analysis; D013552:Swine; D013696:Temperature; D013997:Time Factors
chemical_list: D001647:Bile Acids and Salts; D010743:Phospholipids; D002784:Cholesterol; D001663:Bilirubin; D002118:Calcium