Identification of exons in a region of human chromosome 6q known to contain tumour suppressor genes.
An important objective in human genomic mapping is the isolation of expressed sequences from defined chromosomal regions. The 6q27 region of the long arm of chromosome 6 is an important target in this endeavour because a number of tumour suppressor genes have been mapped to this region. We have used exon trapping and amplification in conjunction with a chromosome specific genomic library to generate ESTs in 6q27. Nine novel expressed sequences have been identified and these provide additional markers and potential candidate genes for the tumour suppressor genes known to be in 6q27.
Barua
U
U
MRC Human Biochemical Genetics Unit, Wolfson House, London, UK.
Rousseaux
S
S
Edwards
Y H
YH
D000818:Animals; D019556:COS Cells; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D003360:Cosmids; D018076:DNA, Complementary; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014162:Transfection
D018076:DNA, Complementary