title: Laser Doppler measurements of inner ear blood flow during experimental thrombosis of cochlear blood vessels in the guinea pig.
abstract: This study investigated the vascular effect of ferromagnetic obstruction of cochlear blood vessels in the guinea pig using dual-channel laser Doppler flowmetry. To improve this technique, we tested new types of magnets and iron spheres. In so doing, the cochlear temperature was lowered selectively and general hypothermia was avoided. The success of vascular impairment in the inner ear was found to depend on the experimental conditions used. Given normothermic conditions (38 degrees C body temperature), a clear reduction in cochlear blood flow (CBF) was found in only about 30% of the animals tested when an aluminium-nickel-cobalt alloy magnet and carbonyl iron spheres were used, while this ratio increased to about 80% under general hypothermia (33 degrees C). Using a stronger neodymium-iron-boron magnet and smaller-sized iron spheres, we found the success of vascular obstruction to be approximately 70% under normothermia and 100% with local hypothermia (to 33 degrees C) of the cochlea. Although the extent of vascular impairment revealed a considerable interindividual variation, the present findings demonstrate that ferromagnetic intervention in CBF with dual-channel laser Doppler flowmetry can be used to investigate the effect of quantified cochlear ischemia on inner ear physiology in the guinea pig model and test various therapeutic strategies.
authors.affiliation: Universitätsklinikum Charité, Humboldt-Universität Berlin, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Scheibe
authors.forename: H
authors.initials: H
authors.lastname: Haupt
authors.forename: H
authors.initials: H
authors.lastname: Grunert
mesh_terms: D000497:Alloys; D000535:Aluminum; D000818:Animals; D001831:Body Temperature; D001895:Boron; D003035:Cobalt; D003051:Cochlea / Q000098:blood supply*; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000098:blood supply*; D005260:Female; D006168:Guinea Pigs; D007036:Hypothermia, Induced; D007501:Iron; D054354:Iron Carbonyl Compounds; D007511:Ischemia / Q000503:physiopathology; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation / Q000379:methods; D008280:Magnetics; D008297:Male; D008863:Microspheres; D009354:Neodymium; D009532:Nickel; D009942:Organometallic Compounds; D012039:Regional Blood Flow; D013927:Thrombosis / Q000209:etiology / Q000503:physiopathology*
chemical_list: D000497:Alloys; D009942:Organometallic Compounds; D054354:Iron Carbonyl Compounds; D009354:Neodymium; D003035:Cobalt; D009532:Nickel; D000535:Aluminum; D007501:Iron; D001895:Boron