title: An AluI fragment isolated from lake trout (Salvelinus namaycush), maps to the intergenic spacer region of the rDNA cistron.
abstract: The relationship between a 217-bp AluI fragment (SnAluI-33c) from lake trout (Salvelinus namaycush) which hybridizes to the nucleolar organizer regions (NORs) and the ribosomal RNA genes was examined by Southern analysis and comparative hybridization. Restriction enzymes with recognition sites mapped in the lake trout rDNA cistron were used to digest genomic DNA into fragments of predetermined size. Comparison of the hybridization pattern of SnAluI-33c with those of two rDNA-specific probes placed this fragment within the intergenic spacer region of the rDNA cistron, approximately 3 kb upstream (5') of the 18S gene. This finding is consistent with in situ hybridization experiments showing hybridization of this fragment to sites of rDNA [Reed, K.M. and Phillips, R.B., Cytogenet. Cell Genet. 70 (1995) 104-107]. Based on cross hybridization and sequence comparisons, homologous sequences are present in other salmonid species.
authors.affiliation: Department of Biological Sciences, University of Wisconsin-Milwaukee 53201, USA. reed@alpha2.csd.uwm.edu
authors.forename: K M
authors.initials: KM
authors.lastname: Reed
authors.forename: T H
authors.initials: TH
authors.lastname: Oakley
authors.forename: R B
authors.initials: RB
authors.lastname: Phillips
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017124:Conserved Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012474:Salmon / Q000235:genetics; D013045:Species Specificity; D014337:Trout / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases