[Low molecular weight GTPase of the rac family from the bovine retina. Structure of cDNA and its expression in a prokaryotic system].
cDNA with a high degree of homology to the rac1 gene of the Ras-like protein from the HL-60 cell line was isolated from a bovine retina cDNA library. The homologies of the nucleotide and deduced amino acid sequences were 90 and 98%, respectively. The product of cDNA expression in E. coli cells purified by chromatography displayed GTP-binding and GTPase activities. The data suggest that the Rac1 protein is one of the low-molecular GTP-binding proteins in photoreceptor membranes of vertebrates.
Petrov
V M
VM
Tkachuk
S V
SV
Lipkin
V M
VM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018076:DNA, Complementary / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D020558:GTP Phosphohydrolases / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics; D020690:GTPase-Activating Proteins; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000235:genetics*; D012160:Retina / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D020703:ras GTPase-Activating Proteins
D018076:DNA, Complementary; D020690:GTPase-Activating Proteins; D011506:Proteins; D020703:ras GTPase-Activating Proteins; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins
Nizkomolekuliarnaia GTP-aza semeĭstva rac iz setchatki byka. Struktura kDNK i ee ékspressiia v prokarioticheskoĭ sisteme.