title: Magnesium ions are required for HEp-2 cell adhesion by enteroaggregative strains of Escherichia coli O126:H27 and O44:H18.
abstract: Enteroaggregative strains of Escherichia coli, belonging to serotypes O44:H18 and O126:H27, were used to show that magnesium ions were essential for the adhesion of these enteroaggregative strains to HEp-2 cells. The removal of Mg2+ ions from culture media was correlated with the inability of strains to produce an outer membrane-associated protein of 18 kDa and a pellicle. It was concluded that magnesium ions were directly involved with the expression of an 18 kDa outer membrane-associated protein by strains of E. coli O126:H27 and O44:H18, and that the outer membrane-associated protein was involved in both HEp-2 adhesion and pellicle formation.
authors.affiliation: Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
authors.forename: H
authors.initials: H
authors.lastname: Chart
authors.forename: J
authors.initials: J
authors.lastname: Spencer
authors.forename: H R
authors.initials: HR
authors.lastname: Smith
authors.forename: B
authors.initials: B
authors.lastname: Rowe
mesh_terms: D000918:Antibody Specificity; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000502:physiology; D006528:Carcinoma, Hepatocellular; D002460:Cell Line / Q000382:microbiology; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000145:classification / Q000187:drug effects*; D006801:Humans; D015151:Immunoblotting; D008274:Magnesium / Q000494:pharmacology*; D008970:Molecular Weight
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002614:Chelating Agents; C026058:EDDA; D004492:Edetic Acid; D008274:Magnesium