title: Cloning and characterization of a human leptin receptor using a biologically active leptin immunoadhesin.
abstract: Leptin, the product of the ob gene, is a hormone secreted by fat cells which is primarily involved in the regulation of body weight. We have generated a leptin immunoadhesin (leptin-IgG) which was more potent than leptin alone at reducing body weight and food intake when injected into ob/ob mice. This molecule was used to identify high affinity binding sites on human embryonic 293 kidney cells and subsequently to isolate a cDNA encoding the leptin receptor from this cell line by expression cloning. This receptor corresponds to the short form of the recently isolated leptin receptor. Analysis of the expression pattern of the two forms of receptor by Northern blot, in situ hybridization and quantitative PCR showed that the receptor is expressed in most tissues but that the long form is prevalent in the hypothalamus.
authors.affiliation: Department of Molecular Oncology, Genentech Inc., South San Francisco, California 94080, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Luoh
authors.forename: F
authors.initials: F
authors.lastname: Di Marco
authors.forename: N
authors.initials: N
authors.lastname: Levin
authors.forename: M
authors.initials: M
authors.lastname: Armanini
authors.forename: M H
authors.initials: MH
authors.lastname: Xie
authors.forename: C
authors.initials: C
authors.lastname: Nelson
authors.forename: G L
authors.initials: GL
authors.lastname: Bennett
authors.forename: M
authors.initials: M
authors.lastname: Williams
authors.forename: S A
authors.initials: SA
authors.lastname: Spencer
authors.forename: A
authors.initials: A
authors.lastname: Gurney
authors.forename: F J
authors.initials: FJ
authors.lastname: de Sauvage
mesh_terms: D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D017403:In Situ Hybridization; D020738:Leptin; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D007074:Immunoglobulin G; C515448:LEPR protein, human; D020738:Leptin; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D011993:Recombinant Fusion Proteins; C515447:leptin receptor, mouse