title: Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) in toxicological analysis. Studies on the detection of clobenzorex and its metabolites within a systematic toxicological analysis procedure by GC-MS and by immunoassay and studies on the detection of alpha- and beta-amanitin in urine by atmospheric pressure ionization electrospray LC-MS.
abstract: GC-MS is the method of choice for toxicological analysis of toxicants volatile in GC while non-volatile and/or thermally labile toxicants need LC-MS for their determination. Studies are presented on the toxicological detection of the amphetamine-like anorectic clobenzorex in urine by GC-MS after acid hydrolysis, extraction and acetylation and by fluorescence polarization immunoassay (FPIA, TDx (meth)amphetamine II). After ingestion of 60 mg of clobenzorex, the parent compound and/or its metabolites could be detected by GC-MS for up to 84 h or by FPIA for up to 60 h. Since clobenzorex shows no cross-reactivity with the used immunoassay, the N-dealkylated metabolite amphetamine is responsible for the positive TDx results. The intake of clobenzorex instead of amphetamine can be differentiated by GC-MS detection of hydroxyclobenzorex which is detectable for at least as long as amphetamine. In addition, the described GC-MS procedure allows the simultaneous detection of most of the toxicologically relevant drugs. Furthermore, studies are described on the atmospheric pressure ionization electrospray LC-MS detection of alpha- and beta-amanitin, toxic peptides of amanita mushrooms, in urine after solid-phase extraction on RP-18 columns. Using the single ion monitoring mode with the ions m/z 919 and 920 the amanitins could be detected down to 10 ng/ml of urine which allows us to diagnose intoxications with amanita mushrooms.
authors.affiliation: Department of Toxicology, University of Saarland, Homburg (Saar), Germany.
authors.forename: H H
authors.initials: HH
authors.lastname: Maurer
authors.forename: T
authors.initials: T
authors.lastname: Kraemer
authors.forename: O
authors.initials: O
authors.lastname: Ledvinka
authors.forename: C J
authors.initials: CJ
authors.lastname: Schmitt
authors.forename: A A
authors.initials: AA
authors.lastname: Weber
mesh_terms: D000546:Amanitins / Q000652:urine*; D000662:Amphetamines / Q000652:urine*; D016231:Fluorescence Polarization Immunoassay; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D013997:Time Factors; D014116:Toxicology / Q000379:methods*
chemical_list: D000546:Amanitins; D000662:Amphetamines; C049197:beta-amanitin; C000490:clobenzorex