Development and validation of high-performance liquid chromatographic method for the determination of folic acid.
A high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 278 nm is presented for the determination of folic acid with 5-hydroxyindole-3-acetic acid as internal standard. Chromatographic separation was based on ion-pair HPLC with tetrabutylammonium hydroxide as the ion-pairing compound. The method is sensitive, lower limit of detection was 0.1 ng/ml, the limit of quantitation was 0.25 ng/ml, using a 25-microliter sample volume. The systems precision intra- and interassay CVs for folic acid were 1.4% and 2.5%, respectively.
Nagy
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E
Department of Pharmacology, University Medical School of Debrecen, Hungary.
Mezey
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G
Kemecsei
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E
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005492:Folic Acid / Q000032:analysis*; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions
D012996:Solutions; D006897:Hydroxyindoleacetic Acid; D005492:Folic Acid