title: Hydrodynamic properties of nitrogenase--the MoFe protein from Azotobacter vinelandii studied by dynamic light scattering and hydrodynamic modelling.
abstract: Experimental results for the nitrogenase MoFe protein from Azotobacter vinelandii obtained by dynamic light scattering (DLS) are presented. The translational diffusion coefficient was determined to D = (4.0 +/- 0.2) x 10(-7) cm2/s. Complementary, we have performed hydrodynamic model calculations based on the X-ray crystallographic data of the MoFe protein. The calculated transport coefficient suggests that the size and shape of the protein in solution is consistent with that in the crystal structure.
authors.affiliation: Universität Bielefeld, Fakultät für Chemie, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Hellweg
authors.forename: W
authors.initials: W
authors.lastname: Eimer
authors.forename: E
authors.initials: E
authors.lastname: Krahn
authors.forename: K
authors.initials: K
authors.lastname: Schneider
authors.forename: A
authors.initials: A
authors.lastname: Müller
mesh_terms: D016948:Azotobacter vinelandii / Q000201:enzymology*; D018360:Crystallography, X-Ray; D008027:Light; D008958:Models, Molecular; D015394:Molecular Structure; D008983:Molybdoferredoxin / Q000737:chemistry*; D011487:Protein Conformation; D012542:Scattering, Radiation
chemical_list: D008983:Molybdoferredoxin