Cloning and sequencing of ribosomal protein L27a and a gene similar to human GS1 in Drosophila.
Two closely linked genes were identified and characterized in the 24F region on the left arm of chromosome 2 in Drosophila. One cDNA predicts a protein of 231 amino acids, with a molecular mass of 25.7 kDa. The predicted amino-acid sequence of this protein is 47.2% identical to that of the previously reported human GS1 protein, which is encoded by a gene that is of interest because it is one of the few X-linked genes that escapes X-inactivation. We have accordingly named our gene GS1like (GS1l). The second cDNA begins 383 bp proximal to the first. This cDNA encodes a protein of a predicted 149 amino acids and a molecular mass of 17.0 kDa. This protein represents a homolog of ribosomal protein L27a; thus, we have named the gene RpL27a. This gene might be responsible for the Minute mutation located at 24F. An rpL27a gene was previously localized to 87F/88A; thus, this gene might be present in two locations in Drosophila.
Soehnge
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Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77252, USA.
Huang
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Becker
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Conover
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D
Stern
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009708:Nucleotidases; D011506:Proteins / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D029721:Drosophila Proteins; D011506:Proteins; D012269:Ribosomal Proteins; C486159:RpL27A protein, Drosophila; D009708:Nucleotidases; C104955:PUDP protein, human