Radiolabelling leukocytes with 99Tcm-stannous chloride colloid for the detection of inflammatory lesions.
Freshly prepared 99Tcm-stannous chloride colloid (99Tcm-SCC) was used to label human leukocytes. The radiolabelled leukocytes were then injected intravenously into rats bearing carrageenan-induced inflammation in their hindlimbs. Scintigraphic imaging (n = 3) and biodistribution studies (n = 4) 4 h post-injection were performed. The ratio of radioactivity (cpm/pixel) accumulated in the inflammation site to that in the corresponding area of the contralateral limb was 2.9 +/- 0.8, as calculated by region-of-interest analyses of the planar images. The distribution of radioactivity in the dissected tissue showed that the ratio of radioactivity (cpm/g) of infected tissue to that of a normal sample obtained from the contralateral limb was 2.6 +/- 0.5. The biodistribution study of dissected organs showed high uptake in liver (58.9 +/- 8.2%), spleen (4.3 +/- 1.8%) and lungs (6.3 +/- 2.4%). The results of this preliminary study indicate that 99Tcm-SCC can be used as an alternative agent for radiolabelling leukocytes for the detection of inflammatory lesions.
Yu
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CC
Nuclear Medicine Service, Brockton/West Roxbury Veterans Affairs Medical Center, MA 02132, USA.
Tow
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DE
Ahn
C S
CS
Syravanh
C
C
D000818:Animals; D002351:Carrageenan; D002470:Cell Survival; D015902:Gamma Cameras; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000000981:diagnostic imaging*; D017708:Leukocyte Transfusion; D007962:Leukocytes / Q000166:cytology; D015609:Organotechnetium Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D051381:Rats; D017971:Tin Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014343:Trypan Blue
D015609:Organotechnetium Compounds; D017971:Tin Compounds; C105829:technetium Tc 99m stannous chloride; D002351:Carrageenan; D014343:Trypan Blue