In vitro assessment of acetic-acid-soluble proteins (glutenin) toxicity in celiac disease.
Acetic-acid-soluble storage proteins from gluten of the bread wheat cv. Sprint 3 were fractionated by adsorption chromatography on 2000 A controlled-pore glass (CPG) beads, and glutenin polymers with molecular mass higher than 10(7) Da and free from monomeric gliadins were recovered. The glutenin polymers were found to consist of high-molecular-weight (HMW) and low-molecular-weight (LMW) glutenin subunits. Peptic-tryptic (PT) digests of glutenins were examined for their agglutination activity on human myelogenous leukemia K 562(S) cells, agglutination being strongly correlated with toxicity for the celiac intestine. The peptide fraction at a concentration of 1 g/L of culture medium was able to agglutinate 30% of K 562(S) cells, suggesting a moderate toxic effect. This toxicity may be accounted for by homologies in amino acid sequences between glutenin subunits and alpha/beta- and gamma-gliadins.
De Vincenzi
M
M
Laboratorio di Metabolismo e Biochimica Patologica, Istituto Superiore di Sanità, Roma, Italy.
Luchetti
R
R
Peruffo
A D
AD
Curioni
A
A
Pogna
N E
NE
Gasbarrini
G
G
D019342:Acetic Acid / Q000737:chemistry; D000327:Adsorption; D000372:Agglutination Tests; D000595:Amino Acid Sequence; D002446:Celiac Disease / Q000378:metabolism* / Q000503:physiopathology; D005591:Chemical Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D005898:Glass; D005903:Gliadin / Q000302:isolation & purification / Q000378:metabolism; D005983:Glutens / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology; D008863:Microspheres; D008970:Molecular Weight; D011108:Polymers; D016062:Porosity; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000378:metabolism
D011108:Polymers; D005983:Glutens; D005903:Gliadin; C037302:glutenin; D019342:Acetic Acid