title: A molecular predator and its prey: coupled isothermal amplification of nucleic acids.
abstract: BACKGROUND
  A novel approach to the study of in vitro evolution is provided by the investigation of continuous, functionally coupled, amplifying systems. To date, in vitro evolution experiments have focused on issues of mutation and selection. Our work contributes to the new field of in vitro molecular ecology studies in which detailed information about the relationship between sequence changes and molecular interactions is obtained. Predator-prey systems are interesting in this context both in terms of evolutionary limits and in terms of the potential kinetic properties of oscillation and spatial pattern formation. Such molecular predator-prey models can be extended to a further negative-interaction mode, viral-host molecular evolution.
  
  
  RESULTS
  A simple, nonfunctional predator-prey system based on the self-sustained sequence replication reaction is proposed. Coupling within the system is achieved using the single-stranded DNA intermediate of one cycle, the prey cycle, as primer for the second one, the predator cycle. Hybridization by complementary base pairing is the second order reaction step underlying the predation. Single steps of the whole reaction system have been investigated by radiolabeling. Each isolated subsystem operates according to the proposed reaction scheme, and evidence for an efficient coupling of both subsystems according to the proposed mechanism was found.
  
  
  CONCLUSIONS
  Simple, interacting model systems based on nucleic acids can be designed and constructed for the study of coevolution. The results of studies such as the one described here will provide a basis for the construction of coupled systems of ribozymes, from which point the engineering of catalytic units for applications in biotechnology is feasible.
authors.affiliation: Institute for Molecular Biotechnology, Jena, Germany. wlotzka@imb-jena.de
authors.forename: B
authors.initials: B
authors.lastname: Wlotzka
authors.forename: J S
authors.initials: JS
authors.lastname: McCaskill
mesh_terms: D001483:Base Sequence; D005075:Biological Evolution; D017931:DNA Primers; D004277:DNA, Single-Stranded; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D008957:Models, Genetic; D008962:Models, Theoretical; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011235:Predatory Behavior; D011401:Promoter Regions, Genetic; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013698:Templates, Genetic; D014764:Viral Proteins
chemical_list: D017931:DNA Primers; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012194:RNA-Directed DNA Polymerase; D012321:DNA-Directed RNA Polymerases