A suppressor of mutations in the region adjacent to iterons of pSC101 ori.
Some single-base changes in a 14-bp region (the downstream region) adjacent to three repeated sequences (iterons) in pSC101 ori are very deleterious for replication. We isolated a host suppressor mutation for one of these mutations and found that the suppressor suppressed all the mutations tested in the downstream region. The nucleotide sequence of the suppressor revealed that the suppressor gene was identical to dksA, which encodes a multicopy suppressor of the heat shock gene dnaK.
Ohkubo
S
S
Institute for Gene Research, Kanazawa University, Japan.
Yamaguchi
K
K
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D016153:Genes, Suppressor; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D018741:Replication Origin; D013489:Suppression, Genetic; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C090751:dksA protein, E coli