title: Dosimetry of glycidyl ethers in mice by quantification of haemoglobin adducts.
abstract: Glycidyl ethers are used in epoxy resins. Epoxy resins are widely used in adhesives and coatings, as well as in electronics and structural composites, thus there is a potential of human exposure to glycidyl ethers. The aim of the present investigation was to explore the utility of haemoglobin adducts for biomonitoring of these types of compounds. Adducts to N-terminal valine were analysed by a modified Edman method with gas chromatographymass spectrometry (or tandem mass spectrometry) for adduct detection and quantification. Groups of three male mice (C3H/Hej) were administered 4 mg/mouse of allyl, butyl, phenyl or cresyl glycidyl ether (AGE, BGE, PGE or CGE) by i.p. injection. Blood samples were collected 24 h after treatment and assayed for haemoglobin adducts using the N-alkyl Edman method. Additional groups of AGE-treated mice were used to study dose response and adduct stability. The experiments with AGE indicate a linear dose response for adduct formation in the dose range studied (0, 2 and 4 mg/mouse). As expected for stable haemoglobin adducts, about 50% of the initial adduct level remained 21 days after exposure.
authors.affiliation: Department of Radiobiology, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
authors.forename: H L
authors.initials: HL
authors.lastname: Pérez
authors.forename: K
authors.initials: K
authors.lastname: Plná
authors.forename: S
authors.initials: S
authors.lastname: Osterman-Golkar
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D004852:Epoxy Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H
chemical_list: D004852:Epoxy Compounds; D006454:Hemoglobins; C020344:glycidyl ethers