A routine HPLC method for monitoring midazolam in serum.
A routine high-performance liquid chromatographic method for measuring midazolam in human serum has been developed. The sample preparation procedure consisted of simple liquid-liquid extraction with dichloromethane, followed by evaporation under nitrogen. The mobile phase used was a mixture of acetonitrile at 0.02 M and sodium acetate at pH 3.0 (80:20, v/v) and a flow-rate of 1.2 mL/min. The separation was performed on two cyanopropyl columns (150 x 4.6 mm). The detection was by UV absorption at 240 nm. A linear range from 10 to 1,000 ng/mL and a quantification limit of 7.4 ng/mL of serum was reached. The mean intra-assay and inter-assay reproducibility from serum sample spiked with 100 ng/mL were 4.1 and 4.7%, respectively. The recoveries from serum sample spiked with 50, 100, 500 ng/mL were 85.46, 85.38 and 85.57%, respectively. This method was developed to allow pharmacokinetics study of midazolam in young patients in short surgical interventions.
Odou
P
P
Department of Clinical Pharmacy, Faculty of Pharmaceutical and Biological Sciences, University Lille II, France.
Robert
H
H
Luyckx
M
M
Brunet
C
C
Dine
T
T
Gressier
B
B
Cazin
M
M
Cazin
J C
JC
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood*; D008752:Methylene Chloride; D008832:Microchemistry; D008874:Midazolam / Q000097:blood*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006993:Hypnotics and Sedatives; D008752:Methylene Chloride; D008874:Midazolam