[PCR-detection of cytomegalovirus infection in oncologic and non-oncologic patients with organ and tissue transplantation].
Cytomegalovirus (CMV) infection often causes death after organ transplantation. Therefore, adequate prevention, diagnosis and treatment of this complication are of critical importance. The experience of establishing routine PCR-detection of CMV is presented. A widely accepted PCR protocol was used for clinical purposes. The strategy included 3 steps: 1) PCR amplification of CMV-sequences from leukocytes of patients; 2) visualization of PCR-product in polyacrylamide gel; 3) verification of the identity of PCR product by its hybridization to a membrane-bound specific probe. This approach allowed to detect CMV infection in 10 out of 28 bone marrow and in 4 out of 32 kidney recipients. The administration of specific antiviral treatment led to the elimination of CMV sequences from the blood.
Aleksandrova
I N
IN
Koshkarova
I V
IV
Lyshchev
A A
AA
Sitskaia
K O
KO
Zubarovskaia
L S
LS
Akimova
S L
SL
Shabanova
L N
LN
Pluzhnikova
G F
GF
Rudenko
V I
VI
Khanson
K P
KP
Afanas'ev
B V
BV
D000998:Antiviral Agents / Q000627:therapeutic use; D016026:Bone Marrow Transplantation; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification*; D006801:Humans; D016030:Kidney Transplantation; D014184:Transplantation, Homologous
D000998:Antiviral Agents; D015342:DNA Probes; D004279:DNA, Viral
PTSR-diagnostika tsitomegalovirusnoĭ infektsii u onkologicheskikh i neonkologicheskikh bol'nykh, perenesshikh transplantatsiiu organov i tkaneĭ.