Substrate specificities of alpha-galactosidases from yeasts.
Twenty-nine strains of yeasts, which are capable of using galactose, melibiose, or raffinose, were screened for alpha-galactosidase production. Among the strains, 5 produced intracellular and extracellular alpha-galactosidases, and 2 produced only intracellular enzyme. Substrate specificities of these enzymes were explored using 6(3)-alpha-D-galactosyl-1,4-beta-D-mannotriose and 6(3)-alpha-D-galactosyl-1,4-beta-D-mannotetraose. All enzymes liberated the terminal galactose from 6(3)-alpha-D-galactosyl-1,4-beta-D-mannotriose, but did not the stub galactose from 6(3)-alpha-D-galactosyl-1,4-beta-D-mannotetraose.
Yoshida
S
S
Institute of Applied Biochemistry, University of Tsukuba, Japan.
Tan
C H
CH
Shimokawa
T
T
Turakainen
H
H
Kusakabe
I
I
D005656:Fungal Proteins / Q000378:metabolism*; D009598:Nitrophenylgalactosides / Q000378:metabolism; D013379:Substrate Specificity; D015003:Yeasts / Q000201:enzymology*; D000519:alpha-Galactosidase / Q000378:metabolism*
D005656:Fungal Proteins; D009598:Nitrophenylgalactosides; C059200:4-nitrophenylgalactoside; D000519:alpha-Galactosidase