The isolation and characterization of an immunomodulatory and anti-tumor polysaccharide preparation from Flammulina velutipes.
Alkaline-soluble antitumor polysaccharide was prepared from the cell wall of the mushroom Flammulina velutipes. The backbones) of the polysaccharide is mainly composed of beta-(1-->3)-D-linked glucose and its molecular weight was estimated to be about 200 kD. The polysaccharide was found to be non-toxic by brine shrimp assay. When injected into mice intraperitoneally, the polysaccharide triggered proliferation of splenic lymphocytes and also vascular dilation and hemorrhage (VDH) response. The polysaccharide exhibited potent anti-tumor activity against sarcoma SC-180 in vivo but not in vitro.
Leung
M Y
MY
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Fung
K P
KP
Choy
Y M
YM
D000276:Adjuvants, Immunologic / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002473:Cell Wall / Q000737:chemistry; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D011134:Polysaccharides / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy; D013154:Spleen / Q000166:cytology
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D011134:Polysaccharides