Direct enantiomeric separation of cathinone and one major metabolite on cellobiohydrolase (CBH-I) chiral stationary phase.
A direct, isocratic high-performance liquid chromatographic (HPLC) method is described for the enantiomeric separation of racemic cathinone and one of its metabolites (+/-)-norephedrine, on a protein-based chiral stationary phase (CSP), cellobiohydrolase, known as CBH-I, under reversed-phase conditions. It was determined that the S-(-)-enantiomer eluted first for cathinone while the 1S,2R-(+)-enantiomer eluted first for norephedrine. The procedure is simple with no sample derivatization required and a short assay time.
Aboul-Enein
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Bioanalytical and Drug Development Laboratory, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
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D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D000697:Central Nervous System Stimulants / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009994:Osmolar Concentration; D010665:Phenylpropanolamine / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
D000470:Alkaloids; D000697:Central Nervous System Stimulants; D010665:Phenylpropanolamine; C023665:cathinone; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase