Development of PCR-SSOP for the identification of HLA-A*02 subtypes and determination of HLA-A*02 frequencies within different ethnic populations.
A PCR-SSOP typing method, involving a single PCR amplification in conjunction with 19 digoxigenin labelled oligonucleotide probes, has been developed for the identification of 17 known HLA-A*02 alleles. The method has been applied to four populations (Northern Ireland, Singapore Chinese, Shetland Island and Mexican) and percentages of HLA-A*02 alleles determined within each population.
Williams
F
F
Northern Ireland Tissue Typing Laboratory, City Hospital, Belfast, Northern Ireland, United Kingdom.
Middleton
D
D
Savage
D
D
Gorodezky
C
C
Wilson
D W
DW
Fitzgerald
J M
JM
Urbaniak
S J
SJ
D000483:Alleles; D002460:Cell Line; D005006:Ethnicity; D005787:Gene Frequency; D015789:HLA-A2 Antigen / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing; D016133:Polymerase Chain Reaction
D015789:HLA-A2 Antigen