Linkage map of Syrian hamster with restriction landmark genomic scanning.
We have constructed the linkage map with precise genetic analysis of the Syrian hamster, Mesocricetus auratus, according to the restriction landmark genomic scanning (RLGS) spot mapping method. Although only 3.2-6.6% of the total RLGS spots between the two strains, ACN and BIO 14.6, showed genetic variance, 572 loci were found to be polymorphic. Out of 569 RLGS loci and 3 other loci, 531 were mapped with the backcross (ACN x BIO 14.6) F1 x BIO 14.6. The cumulative map was 1111.6 cM, indicating that the spots/loci are located throughout the genome at 1.94 cM intervals on average. Thus, RLGS provides us with a rapid tool to construct the genetic map of any species, even if it has less genetic variation.
Okuizumi
H
H
Genome Science Laboratory, Tsukuba Life Science Center, Ibaraki, Japan.
Ohsumi
T
T
Sakaki
N
N
Imoto
H
H
Mizuno
Y
Y
Hanami
T
T
Yamashita
H
H
Kamiya
M
M
Takada
S
S
Kitamura
A
A
Muramatsu
M
M
Nishimura
M
M
Mori
M
M
Matsuda
Y
Y
Tagaya
O
O
Okazaki
Y
Y
Hayashizaki
Y
Y
D000818:Animals; D006224:Cricetinae; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D008297:Male; D008647:Mesocricetus / Q000235:genetics*; D015183:Restriction Mapping