Direct evidence for uptake of intact liposomes encapsulating silver sulfadiazine by cultured human keratinocytes based on combined transmission electron microscopy and X-ray microanalysis.
Cultured human keratinocytes were exposed to liposomally encapsulated silver sulfadiazine, free silver sulfadiazine, silver sulfadiazine cream, and a corresponding vehicle for 5 min to 24 h. Phagocytosis of intact liposomes by keratinocytes was demonstrated in vitro by combined X-ray microanalysis and electron microscopy. Silver as an active part of the antimicrobial served as an electron-dense marker.
Schaller
M
M
Department of Dermatology, Ludwig Maximilians University, Munich, Germany.
Wurm
R
R
Korting
H C
HC
D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002214:Capsules; D002478:Cells, Cultured; D004337:Drug Carriers; D004577:Electron Probe Microanalysis; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000648:ultrastructure; D008081:Liposomes; D008854:Microscopy, Electron; D009824:Ointments; D010587:Phagocytosis; D012837:Silver Sulfadiazine / Q000008:administration & dosage / Q000493:pharmacokinetics*
D000891:Anti-Infective Agents, Local; D002214:Capsules; D004337:Drug Carriers; D008081:Liposomes; D009824:Ointments; D012837:Silver Sulfadiazine