Determination of omeprazole in human plasma by high-performance liquid chromatography.
A new method for the determination of omeprazole in human plasma was developed. Omeprazole was extracted from plasma with toluene-isoamylalcohol (95:5, v/v), the organic phase was evaporated, dissolved in the mobile phase and injected into a reversed-phase C18 column. Flunitrazepam was used as an internal standard. The mobile phase consisted of 47% methanol and 53% of 0.1 M dipotassium hydrogenphosphate, pH 7.8. The spectrophotometric detection was performed at 302 nm. Limit of quantitation was 9.7 ng/ml and the calibration curve was linear up to 1240 ng/ml.
Macek
J
J
Pharmakl s.r.o., Prague, Czech Republic.
Ptácek
P
P
Klíma
J
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005445:Flunitrazepam; D006801:Humans; D007202:Indicators and Reagents; D009853:Omeprazole / Q000097:blood*; D000439:Pentanols; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents; D014050:Toluene
D007202:Indicators and Reagents; D000439:Pentanols; D012997:Solvents; D014050:Toluene; D005445:Flunitrazepam; C029683:isopentyl alcohol; D009853:Omeprazole