[The sorption of antisense oligodeoxyribonucleotides by Mycoplasma cells].
The paper deals with kinetics of binding of antisense oligodesoxyribonucleotides, complementary to certain sequences 16 S RNA of mollicutes, by the cells of three representatives of class Mollicutes: Acholeplasma laidlawii PG-8. Mycoplasma pneumoniae FH and M. fermentans PG-18. It is shown that binding of antisense oligonucleotides by the mollicute cells depends on temperature and age of cultures. The highest level of sorption of labelled antisense oligodesoxyribonucleotides by the cells of mycoplasmas corresponded to the phase of logarithmic growth of each of the studied mollicute strains. The lengthening of nucleotide chain from 5 to 15 nucleotide bases did not result in the decrease of sorption of the studied oligodesoxyribonucleotides by the mollicute cells.
Egorov
O V
OV
Panchenko
L P
LP
Skripal'
I G
IG
D000042:Absorption; D000128:Acholeplasma laidlawii / Q000378:metabolism*; D001483:Base Sequence; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016858:Mycoplasma fermentans / Q000378:metabolism*; D009177:Mycoplasma pneumoniae / Q000378:metabolism*; D015345:Oligonucleotide Probes; D016376:Oligonucleotides, Antisense / Q000493:pharmacokinetics*; D012329:RNA, Bacterial / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D016376:Oligonucleotides, Antisense; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
Issledovanie sorbtsii antismyslovykh oligodeoksiribonukleotidov kletkami mikoplazm.