Effect of bacteria on survival and growth of Acanthamoeba castellanii.
The growth and survival of Acanthamoeba castellanii in the presence of Gram-negative bacteria such as Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens, and Stenotrophomonas maltophilia varied with the densities and species of bacteria. All species of bacteria suspended in a buffered saline at densities of 10(5) to 10(6)/ml supported the growth and survival of 10(6)/ml trophozoites of Acanthamoeba castellanii in a buffered saline solution. At densities of bacteria to amoebae of 100:1 or greater, growth and survival of A. castellanii were suppressed, particularly by P. aeruginosa. In an enrichment medium, the rapid growth of most co-inoculated bacteria inhibited the growth and survival of the amoeba.
Wang
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Georgia State University, Biology Department, P.O. Box 4010, Atlanta, GA 30303-4010, USA.
Ahearn
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D000048:Acanthamoeba / Q000254:growth & development*; D000818:Animals; D000898:Antibiosis; D018920:Coculture Techniques; D003470:Culture Media / Q000378:metabolism; D004926:Escherichia coli / Q000254:growth & development*; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D012706:Serratia marcescens / Q000254:growth & development*; D014974:Xanthomonas / Q000254:growth & development*
D003470:Culture Media