Compact tunable Cr:LiSAF laser for infrared matrix-assisted laser desorption/ionization.
A tunable Cr:LiSAF laser-pumped optical parametric oscillator was used for mid-infrared matrix-assisted laser desorption/ionization (MALDI) mass spectrometry experiments. The mass spectra of substance P, bovine insulin and pd(T)10 in the 2.88-2.96 microns range showed excellent single shot signal quality (signal-to-noise-ratio, resolution) but poor shot-to-shot reproducibility. The reproducibility is expected to improve with more stable laser design. No correlation was found between the absorption spectrum of the matrix and the MALDI response.
Sadeghi
M
M
Department of Chemistry, George Washington University, Washington, D.C. 20052, USA.
Olumee
Z
Z
Tang
X
X
Vertes
A
A
Jiang
Z X
ZX
Henderson
A J
AJ
Lee
H S
HS
Prasad
C R
CR
D000818:Animals; D002417:Cattle; D007259:Infrared Rays; D007328:Insulin / Q000032:analysis; D007834:Lasers; D009696:Nucleic Acids / Q000032:analysis; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation*; D013055:Spectrophotometry, Infrared; D013373:Substance P / Q000032:analysis
D007328:Insulin; D009696:Nucleic Acids; D013373:Substance P