Biochemical and genetic characterization of Rbf1p, a putative transcription factor of Candida albicans.
A Candida albicans gene encoding a novel DNA-binding protein that bound to the RPG box of Saccharomyces cerevisiae and the telomeric repeat sequence of C. albicans was previously cloned and designated RBF1 (RPG-box-binding factor). In this report, determination of the functional domains of the protein is described. The DNA-binding domain was 140 aa in length, was centrally located between two glutamine-rich regions, and correlated with transcriptional activation in S. cerevisiae. The results, together with the previous finding that showed its predominant localization in the nucleus, suggest that this DNA-binding protein could be a transcription factor. Disruption of the functional RBF1 gene of C. albicans strains caused an alteration in cell morphology to the filamentous form on all solid and liquid media tested. Thus, we speculate that Rbf1p may be involved in the regulation of the transition between yeast and filamentous forms at the level of transcription.
Ishii
Nobuya
N
Department of Mycology, Nippon Roche Research Center, Kajiwara 200, Kamakura, Kanagawa-Prefecture, Japan.
Yamamoto
Mutumi
M
Department of Mycology, Nippon Roche Research Center, Kajiwara 200, Kamakura, Kanagawa-Prefecture, Japan.
Yoshihara
Fumie
F
Department of Mycology, Nippon Roche Research Center, Kajiwara 200, Kamakura, Kanagawa-Prefecture, Japan.
Arisawa
Mikio
M
Department of Mycology, Nippon Roche Research Center, Kajiwara 200, Kamakura, Kanagawa-Prefecture, Japan.
Aoki
Yuhko
Y
Department of Mycology, Nippon Roche Research Center, Kajiwara 200, Kamakura, Kanagawa-Prefecture, Japan.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001665:Binding Sites; D002176:Candida albicans / Q000235:genetics*; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005973:Glutamine; D009024:Morphogenesis / Q000235:genetics; D009687:Nuclear Proteins; D011487:Protein Conformation; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics*
C104798:AlgK protein, Pseudomonas aeruginosa; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; C104796:RBF1 protein, Candida albicans; D014157:Transcription Factors; D005973:Glutamine