Characterization of Serpulina hyodysenteriae isolates of serotypes 8 and 9 by random amplification of polymorphic DNA analysis.
A PCR-based DNA fingerprinting method termed RAPD (Random Amplification of Polymorphic DNA), or AP-PCR (for Arbitrary Primed PCR) was used to detect sequence diversity among reference strains and isolates of Serpulina hyodysenteriae. RAPD fingerprinting of 20 S. hyodysenteriae isolates of serotypes 8 or 9 from Quebec was generated with 2 different 10-base primers used independently. Reference strains and field isolates belonging to serotypes 8 or 9 revealed polymorphisms in RAPD fingerprints with both primers. Interspecies polymorphisms were observed by RAPD analysis of S. hyodysenteriae representing serotypes 1 to 9, S.innocens, and 5 other weakly beta-hemolytic intestinal spirochetes. A dendrogram based on the analysis of RAPD profiles of the strains tested with one of the primers (#17), permitted the clustering of these strains into 11 divisions. The predominance of particular RAPD profiles among S. hyodysenteriae isolates isolated from cases of swine dysentery in different herds suggested that certain S. hyodysenteriae types could be epidemiologically important. Our results indicate that RAPD could be used as a typing method for S. hyodysenteriae and as an epidemiological method for identifying spirochetes isolated from swine.
Dugourd
D
D
Groupe de Recherche sur les Maladies Infectieuses du Porc Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Qué., Canada.
Jacques
M
M
Bigras-Poulin
M
M
Harel
J
J
D001483:Base Sequence; D017401:Brachyspira hyodysenteriae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012016:Reference Values; D012703:Serotyping; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial