title: Overexpression of intercellular adhesion molecule-1 (ICAM-1) in pancreatic adenocarcinoma in comparison with normal pancreas.
abstract: To elucidate the role of intercellular adhesion molecules (ICAMs), which has not been well understood in pancreas, we investigated the localization and expression of ICAM-1 by immunohistochemistry and in situ hybridization (ISH) in pancreatic adenocarcinoma and in normal pancreas. The localizations of ICAM-2 and ICAM-3 were also investigated by immunohistochemistry. In normal pancreas, acinar cells, duct epithelial cells, and Langerhans islet cells failed to stain with anti-ICAM-1, anti-ICAM-2, and anti-ICAM-3 antibodies. These cells showed no expression of ICAM-1 mRNA. On the other hand, various percentages of carcinoma cells were stained with anti-ICAM-1 antibody, while no carcinoma cells were stained with anti-ICAM-2 and anti-ICAM-3 antibodies. ICAM-1 mRNA expression was also observed in carcinoma cells, and ICAM-1 mRNA expression was associated with localization of the ICAM-1 protein. These results suggest that ICAM-1 expression is up-regulated in pancreatic adenocarcinoma cells and that ICAM-1 is involved in malignant processes in pancreas.
authors.affiliation: Department of General Surgery, University of Ulm, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Shimoyama
authors.forename: F
authors.initials: F
authors.lastname: Gansauge
authors.forename: S
authors.initials: S
authors.lastname: Gansauge
authors.forename: U
authors.initials: U
authors.lastname: Widmaier
authors.forename: T
authors.initials: T
authors.lastname: Oohara
authors.forename: H G
authors.initials: HG
authors.lastname: Beger
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000378:metabolism*; D015703:Antigens, CD / Q000032:analysis; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules / Q000032:analysis; D015342:DNA Probes; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis* / Q000235:genetics*; D010179:Pancreas / Q000737:chemistry / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; D015342:DNA Probes; C065289:ICAM2 protein, human; C483616:ICAM3 protein, human; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1