Identification of two palmitoyl groups in octopus rhodopsin.
We determined the structure and site of fatty acid incorporated in octopus rhodopsin using a combination of fluorescence label and enzymatic cleavage methods in conjunction with fast-atom bombardment (FAB) mass spectrometry. A single peptide containing two adjacent cysteines, Cys337 and Cys338, was successfully isolated using the fluorescence from a dye conjugated to Cys345. The FAB mass spectrometric analysis of the peptide (323Phe-340Phe) revealed that two palmitoyl groups are linked to Cys337 and Cys338 via thioester bonds in octopus rhodopsin as in bovine rhodopsin.
Nakagawa
M
M
Department of Life Science, Himeji Institute of Technology, Hyogo, Japan.
Iwasa
T
T
Kikkawa
S
S
Takao
T
T
Shimonishi
Y
Y
Tsuda
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D006031:Glycosylation; D008969:Molecular Sequence Data; D049831:Octopodiformes; D019308:Palmitic Acid / Q000737:chemistry*; D012243:Rhodopsin / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D019308:Palmitic Acid; D012243:Rhodopsin