title: The detection of gene mutation in the tubular sperm of Muta Mice following a single intraperitoneal treatment with methyl methanesulphonate or ethylnitrosourea.
abstract: Transgenic mouse assays, such as Muta Mouse, provide a method to predict the potential target organ carcinogenicity of chemical compounds. As part of a collaborative study, the effects of the direct-acting mutagens, methyl methanesulphonate (MMS) and ethylnitrosourea (ENU), were investigated for gene mutation in the tubular sperm of Muta Mice testes after a single intraperitoneal exposure. Groups of male Muta Mice were dosed intraperitoneally with either 1/15 M phosphate buffer, pH 6.0 (vehicle control), 40 mg/kg methyl methanesulphonate (MMS) or 150 mg/kg ethylnitrosourea (ENU). The animals were sacrificed 14 days after the single dose. Mutation frequencies were determined in tubular sperm DNA. The results showed a mean mutation frequency (MF) of 2.1 x 10(5) (64 mutants per 3.05 x 10(6) PFU) for the 10 vehicle-treated mice, a mean MF of 2.8 x 10(5) (78 mutants per 2.75 x 10(6) PFU) for the 10 MMS-treated mice and a mean MF of 9.1 x 10(5) (194 mutants per 2.14 x 10(6) PFU) for the 8 ENU-treated mice; this latter value representing a 4.5-fold increase over the vehicle control values.
authors.affiliation: Corning Hazleton (Europe), North Yorkshire, UK.
authors.forename: T M
authors.initials: TM
authors.lastname: Brooks
authors.forename: S W
authors.initials: SW
authors.lastname: Dean
mesh_terms: D000818:Animals; D004247:DNA / Q000187:drug effects / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005038:Ethylnitrosourea / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D051379:Mice; D008822:Mice, Transgenic; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012671:Seminiferous Tubules / Q000166:cytology; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D009153:Mutagens; D004247:DNA; D008741:Methyl Methanesulfonate; D005038:Ethylnitrosourea