title: Manganese-dependent Dopa/tyrosine sulfation in HepG2 human hepatoma cells: novel Dopa/tyrosine sulfotransferase activities associated with the human monoamine-form phenol sulfotransferase.
abstract: Human monoamine (M)-form phenol sulfotransferase (PST) was PCR-cloned and transiently expressed in COS-7 cells. The recombinant enzyme was demonstrated to display not only the previously reported sulfotransferase activity toward dopamine, but also novel manganese-dependent Dopa/tyrosine sulfotransferase activities. These results imply a new functional role of the human M-form PST in the homeostatic regulation of Dopa and tyrosine.
authors.affiliation: Department of Biochemistry, University of Texas Health Center at Tyler 75710, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: J
authors.initials: J
authors.lastname: Katafuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Takami
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Suiko
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
authors.forename: M C
authors.initials: MC
authors.lastname: Liu
mesh_terms: D000818:Animals; D015239:Arylsulfotransferase / Q000378:metabolism; D019556:COS Cells; D015246:Deoxyribonuclease EcoRI; D006801:Humans; D008345:Manganese / Q000378:metabolism* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D015238:Sulfotransferases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D011994:Recombinant Proteins; D008345:Manganese; C097311:Dopa-tyrosine sulfotransferase; D015238:Sulfotransferases; D015239:Arylsulfotransferase; D015246:Deoxyribonuclease EcoRI