Phosphodiesterase specific inhibitors control cell growth of a human neuroepithelioma cell line.
The effects of cyclic nucleotide phosphodiesterase (PDE) inhibitors on cell proliferation of SK-N-MC human neuroepithelioma cell line was studied. Clonal density experiments in the presence of 100 microM rolipram and zaprinast showed respectively 27% and 91% inhibition. The effects of PDE inhibitors were then investigated on crude cell extracts; the calculated IC50 were 32 microM for zaprinast and 16 nM for DC-TA-46; the latter inhibitor was used instead of rolipram for its higher efficacy. Dose-response experiments in clonal density conditions showed IC50 of 5 microM and 1.8 microM in the presence respectively of zaprinast and rolipram. These data show that both inhibitors are effective in reducing cell growth, although the response was quantitatively different.
Campagnolo
L
L
Dip. Biol. Cellulare e dello Sviluppo, Università di Roma La Sapienza, Italy.
Giorgi
M
M
Augusti-Tocco
G
G
D002455:Cell Division / Q000187:drug effects; D006801:Humans; D018241:Neuroectodermal Tumors, Primitive, Peripheral; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D011621:Pteridines / Q000494:pharmacology*; D011688:Purinones / Q000494:pharmacology*; D011760:Pyrrolidinones / Q000494:pharmacology*; D020889:Rolipram; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011621:Pteridines; D011688:Purinones; D011760:Pyrrolidinones; C081896:7-benzylamino-6-chloro-2-piperazino-4-pyrrolidinopteridine; C011145:zaprinast; D020889:Rolipram