Overexpression of glutathione S-transferase pi messenger RNA and its relationship to gene amplification in head and neck squamous cell carcinoma.
Human glutathione S-transferase pi has been known to be a good marker for several tumor types because of the high frequency with which it is overexpressed. In order to determine whether GST pi is useful as an indicator for head and neck cancers, expression of GST pi was investigated by Northern analysis. Overexpression of mRNA was detected in 9 of 36 primary head and neck squamous cell carcinomas. To examine the relationship between overexpression and amplification of GST pi gene, Southern analysis was performed on all samples. Only 3 of the 36 tumors showed amplification GST pi genes, indicating that gene amplification may not play a key role in GST pi mRNA overexpression in these cancers.
Pavelic
Z P
ZP
Department of Otolaryngology-Head and Neck Surgery, College of Medicine, University of Cincinnati, OH 45267, USA.
Wang
X
X
Li
Y
Y
Gleich
L
L
Gluckman
J L
JL
Stambrook
P J
PJ
D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis; D005784:Gene Amplification; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000032:analysis / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D007822:Laryngeal Neoplasms / Q000201:enzymology / Q000235:genetics; D009062:Mouth Neoplasms / Q000201:enzymology / Q000235:genetics; D009364:Neoplasm Recurrence, Local / Q000201:enzymology / Q000235:genetics; D009367:Neoplasm Staging; D009959:Oropharyngeal Neoplasms / Q000201:enzymology / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000032:analysis
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D007527:Isoenzymes; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase