[Isolation and characterization of Epstein-Barr virus-infected clones of a human T-lymphoblastoid cell line].
Infection of the human T-cell line MT-2 by Epstein-Barr virus(EBV) recombinants with a hygromycin resistance gene and subsequent selection with this drug permitted isolation and long-term maintenance of EBV-infected MT-2 clones. These MT-2 clones harbor monoclonal episomes of EBV DNA and stably express two EBV latent proteins, EBV nuclear antigen 1(EBNA1) and latent membrane protein 1 (LMP1). Some MT-2 clones also express EBNA3C, yet none of the clones express EBNA2. This pattern of EBV gene expression is similar to that observed in most cases of EBV-positive peripheral T-cell lymphoma. In addition to these latent viral functions, expression of some early EBV replicative genes, including BZLF1, was also detected. EBV recombinants with positive selection markers will be a useful tool in establishing experimental systems of persistent EBV infection in certain non-B-cell lineages.
Fujiwara
S
S
Department of Microbiology, Nihon University School of Medicine.
Nakamura
H
H
Iwakiri
D
D
Ono
Y
Y
D002460:Cell Line; D002999:Clone Cells; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000821:virology*; D014764:Viral Proteins / Q000032:analysis
D014764:Viral Proteins