Production of monoclonal antibodies against quail Pax-1.
Monoclonal antibodies (MAbs) against quail Pax-1 were generated using a fusion protein of the C-terminal part of Pax-1 and glutathione S-transferase. The MAbs generated could detect quail Pax-1 protein by Western blotting and immunoprecipitate it from whole cell extracts of quail embryos, indicating their usefulness for biochemical analyses of Pax-1 protein function in development or oncogenesis.
Kaneko
K
K
Division of Molecular Immunology, School of Medicine, Chiba University, Japan.
Taniguchi
M
M
Isono
K
K
Koseki
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007132:Immunoglobulin Isotypes / Q000737:chemistry; D051761:Paired Box Transcription Factors; D011233:Precipitin Tests; D011784:Quail / Q000235:genetics / Q000276:immunology*; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D007132:Immunoglobulin Isotypes; D051761:Paired Box Transcription Factors; D014157:Transcription Factors; C070835:PAX1 transcription factor