Purification and properties of poly(3-hydroxybutyrate) depolymerase from the fungus Paecilomyces lilacinus D218.
Poly(3-hydroxybutyrate) depolymerase was purified to homogeneity from the culture filtrate of Paecilomyces lilacinus D218 by column chromatography on CM-Toyopearl 650M and hydroxylapatite. The molecular weight of the enzyme was estimated to be 48,000 by SDS-PAGE. Maximal activity was observed near pH 7.0 and 45 degrees C. The Km and Vmax values for PHB were 0.13(mg/ml) and 3750 (U/mg protein), respectively. The enzyme hydrolyzed PHB and p-nitrophenyl fatty acids but not polycaprolactone and triglycerides.
Oda
Y
Y
Department of Food Science and Technology, Fukuyama University, Fukuyama, Hiroshima 729-02, Japan.
Osaka
H
H
Urakami
T
T
Tonomura
K
K
D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004229:Dithiothreitol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005033:Ethylmaleimide / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000378:metabolism; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D019216:Metals, Heavy / Q000494:pharmacology; D010143:Paecilomyces / Q000201:enzymology*; D011091:Polyesters / Q000378:metabolism; D011136:Polysorbates / Q000494:pharmacology; D013379:Substrate Specificity; D014105:Tosyl Compounds / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D002265:Carboxylic Ester Hydrolases; D004229:Dithiothreitol; D004492:Edetic Acid; D005033:Ethylmaleimide; D005227:Fatty Acids; D006885:Hydroxybutyrates; D007461:Iodoacetates; D019807:Iodoacetic Acid; D019216:Metals, Heavy; D011091:Polyesters; D011136:Polysorbates; D014105:Tosyl Compounds; D014280:Triglycerides; C016240:polycaprolactone; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C003614:4-toluenesulfonyl fluoride; C022417:poly-beta-hydroxybutyrate depolymerase