Effects of swimming training on brown-adipose-tissue activity in obese ob/ob mice: GDP binding and UCP m-RNA expression.
Brown adipose tissue (BAT) of obese animals is generally in a relatively atrophied and thermogenically quiescent state. The aim of the current study was to investigate the effect of swimming training on BAT activity in lean and obese (ob/ob) mice. The trained mice underwent a 6-week endurance swimming training (1 h/day, 5 days/week) in water at 35-36 degrees C. The swimming training significantly increased BAT mass and its protein content in both the lean and obese mice, suggesting hypertrophy. After swimming training, the amounts of protein and guanosine 5'-diphosphate binding in the mitochondria recovered from BAT of both mice increased significantly as compared with the respective sedentary groups, whereas the uncoupling protein (UCP) content increased significantly only in lean mice. After swimming training, the level of UCP mRNA expression did not change substantially in lean mice but appeared to increase in obese mice. The results obtained here suggest that swimming training leads to an increase in the nonshivering thermogenesis of obese mice in addition to lean mice.
Ueno
N
N
Department of Public Health, Juntendo University, School of Medicine, Tokyo, Japan.
Oh-ishi
S
S
Kizaki
T
T
Nishida
M
M
Ohno
H
H
D002001:Adipose Tissue, Brown / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001835:Body Weight; D002352:Carrier Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D006153:Guanosine Diphosphate / Q000378:metabolism*; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D024101:Mitochondrial Proteins; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D009929:Organ Size; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D000071256:Uncoupling Protein 1; D006153:Guanosine Diphosphate