title: Mutation in a new gene MAF1 affects tRNA suppressor efficiency in Saccharomyces cerevisiae.
abstract: Mutation in the MAF1 gene was identified in a screen for decreased efficiency of tRNA suppressor SUP11 in the yeast Saccharomyces cerevisiae (Sc). maf1-1 mutation exerts a dual phenotypic effect: antisuppression and temperature sensitive (ts) respiratory growth. MAF1, cloned by complementation of the ts phenotype of maf1-1, also alleviates the antisuppressor effect. The coding sequence of MAF1 is interrupted by an intron of 80 bp. The putative gene product, Maf1p, is a hydrophilic protein of 395 amino acids (aa) not showing significant similarity to known proteins which indicates that MAF1 encodes a novel protein. Maf1p may play a role in the tRNA biosynthetic pathway since a fragment of the RPO31/RPC160 gene encoding the largest subunit of RNA polymerase III was cloned as a multicopy suppressor of mafl-1.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland. magda@ibbrain.ibb.waw.pl
authors.forename: M
authors.initials: M
authors.lastname: Boguta
authors.forename: K
authors.initials: K
authors.lastname: Czerska
authors.forename: T
authors.initials: T
authors.lastname: Zoładek
mesh_terms: D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005784:Gene Amplification; D016153:Genes, Suppressor; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D011506:Proteins / Q000235:genetics; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors
chemical_list: D005656:Fungal Proteins; C104954:MAF1 protein, S cerevisiae; D011506:Proteins; C074265:SUP11 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012343:RNA, Transfer; D019883:Alkyl and Aryl Transferases; C068392:MOD5 protein, S cerevisiae; D012320:RNA Polymerase III