Cloning and sequencing of trehalose synthase gene from Thermus aquaticus ATCC33923.
The gene encoding trehalose synthase (catalyzing the conversion of maltose into alpha, alpha-trehalose by transglucosylation) was cloned from Thermus aquaticus ATCC33923. Sequence analysis revealed a 2892 bp synthase gene and a 963 residue amino-acid sequence. The 547 N-terminal residues were homologous to the full-length synthase from Pimelobacter sp. R48 (53.8% identity).
Tsusaki
K
K
Amase Institute, Hayashibara Biochemical Laboratories Inc., Okayama, Japan.
Nishimoto
T
T
Nakada
T
T
Kubota
M
M
Chaen
H
H
Fukuda
S
S
Sugimoto
T
T
Kurimoto
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D013824:Thermus / Q000235:genetics*
D005964:Glucosyltransferases; C099692:trehalose synthase