Immunopotentiating activity of Clostridium butyricum in mice.
Bacterial vaccine, as generated by heat-inactivated Clostridium butyricum cells, displayed antitumor activity against sarcoma 180 in DDY mice and antimetastatic activity against B16-F10 melanoma in BDF1 mice. According to our results, the vaccine has no direct growth inhibitory effect toward the tumor cell lines tested in this study. The vaccine increased gamma-interferon production, elicited delayed type hypersensitivity reaction, and enhanced IgM antibody formation and mitogenicity. The phagocytic activity of macrophage and killing activity of NK cells from mice were enhanced in a dose-dependent manner by stimulating with the heat-inactivated vaccine. Among those responses in the mice treated with CB, elevated NK cell activity may play a prominent role in manifesting antitumor activity in the B16-F10 metastasis experiment.
Wang
G R
GR
Department of Medicine, Taipei Medical College, Japan.
Chen
H Y
HY
Chen
C H
CH
Yeh
M Y
MY
Mikami
Y
Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D001428:Bacterial Vaccines / Q000494:pharmacology / Q000627:therapeutic use*; D003013:Clostridium / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D007111:Immunity, Cellular / Q000187:drug effects; D007075:Immunoglobulin M / Q000096:biosynthesis; D007369:Interferon Inducers / Q000494:pharmacology / Q000627:therapeutic use*; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000187:drug effects; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D008213:Lymphocyte Activation / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control / Q000556:secondary; D051379:Mice; D009368:Neoplasm Transplantation; D010587:Phagocytosis / Q000187:drug effects; D012510:Sarcoma 180 / Q000628:therapy*; D013047:Specific Pathogen-Free Organisms; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D007075:Immunoglobulin M; D007369:Interferon Inducers; D007371:Interferon-gamma