[The production of HIV recombinant proteins and their immunochemical characteristics].
Several Escherichia coli strains producing HIV-specific recombinant proteins including env-1, env-2, and gag sequences have been obtained using different gene engineering techniques. These proteins have been isolated and purified from bacterial lysates by ion exchange chromatography on DEAE-Toyopearl and Streamline-SP columns. Polyacrylamide gel electrophoresis and immunoblotting have been used to prove recombinant proteins purity and to identify them. These proteins have been shown to be adequate as antigen preparations for enzyme immunoassay test-systems aimed at anti-HIV antibodies detection.
Martynenko
D L
DL
Serhieieva
T A
TA
Chumak
R M
RM
Ivans'ka
N V
NV
Zavelevych
M P
MP
D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D015497:HIV-1 / Q000378:metabolism*; D015498:HIV-2 / Q000378:metabolism*; D015151:Immunoblotting; D007120:Immunochemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D014764:Viral Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
D011994:Recombinant Proteins; D014764:Viral Proteins
Oderzhannia rekombinatnykh bilkiv VIL ta ïkh imunokhimichna kharakterystyka.