Oxygen dynamics during partial liquid ventilation in a sheep model of severe respiratory failure.
BACKGROUND
We evaluated the relationship of dose of perflubron and gas tidal volume to oxygen dynamics during partial liquid ventilation in the setting of respiratory failure.


METHODS
Lung injury was induced in 16 sheep by using right atrial injection of 0.15 ml/kg oleic acid. Animals were ventilated with 15 ml/kg gas tidal volume and stabilized. Animals were then divided into three groups: (1) gas ventilation with a tidal volume of 15 ml/kg (control, GV, n = 5); (2) partial liquid ventilation at a gas tidal volume of 15 ml/kg with 10 ml/kg incremental pulmonary dosage of perflubron from 10 to 50 ml/kg (best fill, BF, n = 6); (3) administration of 35 ml/kg perflubron pulmonary dose with 5 ml/kg incremental increase in gas tidal volume from 10 to 30 ml/kg (best tidal volume, BTV, n = 5).


RESULTS
Arterial oxygen saturation increased with increasing dose of perflubron and gas tidal volume (BF, p = 0.01; BTV, p = 0.001). A simultaneous trend toward a reduction in cardiac index was observed with increasing dose of perflubron (BF, p = 0.01). Maximal increase in mixed venous oxygen saturation was observed in the BF and BTV groups at a cumulative perflubron dose of 40 ml/kg and a gas tidal volume of 20 ml/kg, respectively.


CONCLUSIONS
In this sheep lung injury model oxygenation improves with incremental increases in perflubron dose or gas tidal volume, and the mixed venous oxygen saturation appears to be optimal at a cumulative perflubron dose of 40 ml/kg and a gas tidal volume of 20 ml/kg.
Parent
A C
AC
Department of Surgery, University of Michigan, Ann Arbor, USA.
Overbeck
M C
MC
Hirschl
R B
RB
D000818:Animals; D001784:Blood Gas Analysis; D004195:Disease Models, Animal; D005466:Fluorocarbons / Q000494:pharmacology*; D006439:Hemodynamics / Q000187:drug effects; D019301:Oleic Acid; D010100:Oxygen / Q000032:analysis / Q000097:blood*; D010108:Oxyhemoglobins / Q000378:metabolism; D012129:Respiratory Function Tests; D012131:Respiratory Insufficiency / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D012756:Sheep; D013990:Tidal Volume; D014691:Ventilation
D005466:Fluorocarbons; D010108:Oxyhemoglobins; D019301:Oleic Acid; D010100:Oxygen