title: WO-2, a stable aneuploid derivative of Candida albicans strain WO-1, can switch from white to opaque and form hyphae.
abstract: Candida albicans strain WO-2 was isolated as a spontaneous derivative of the white-opaque switching strain WO-1. The electrophoretic karyotype of WO-2 lacks two bands which are found in the parent. These bands correspond to one homologue of chromosome 7 and to a translocation product containing parts of chromosomes 6 and 5. Probing a blot of the karyotype demonstrated that the genetic material in these bands had been lost, yielding an aneuploid strain. UV-irradiation experiments showed that auxotrophs due to mutation in genes located in this region predominated, supporting the conclusion that WO-2 is partially haploid. WO-2 contained about 10% of its genome in the haploid state, and it grew with a doubling time of about twice that of its parent. However, it was able to undergo both the yeast-to-hyphal transition and the white-opaque transition. Hence, these processes do not require perfect diploidy.
authors.affiliation: Department of Genetics and Cell Biology, University of Minnesota, 1445 Gortner Avenue, St Paul, MN 55108, USA.
authors.forename: B B
authors.initials: BB
authors.lastname: Magee
authors.affiliation: Department of Genetics and Cell Biology, University of Minnesota, 1445 Gortner Avenue, St Paul, MN 55108, USA.
authors.forename: P T
authors.initials: PT
authors.lastname: Magee
mesh_terms: D000782:Aneuploidy; D002176:Candida albicans / Q000166:cytology / Q000235:genetics*; D002874:Chromosome Mapping; D015321:Gene Rearrangement; D016681:Genome, Fungal; D009024:Morphogenesis / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic; D014178:Translocation, Genetic