Genomic structure and promoter analysis of the gene encoding MM3, a member of transmembrane 4 superfamily.
We have isolated genomic clones encoding hamster MM3, a member of transmembrane 4 superfamily (TM4SF). Nucleotide (nt) sequence analysis revealed that it is composed of 5 exons spanning about 8 kb. The exon-intron organization of the MM3 gene was quite different from those of other TM4SF members. We also identified its transcription start points (tsp) and the promoter region. Deletion analysis of the promoter revealed that about 160-bp region containing TATA-box, CAAT-box and GC-box was necessary for efficient transcription in cultured cells.
Kurihara
T
T
Department of Virology, University of Tokyo, Japan.
Kataoka
K
K
Hong
D
D
Shioda
S
S
Sugano
S
S
Mitamura
K
K
Maruyama
K
K
Yamaguchi
N
N
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D016385:TATA Box; D014158:Transcription, Genetic
D015703:Antigens, CD; C104953:MM3 protein, Mesocricetus auratus; D008565:Membrane Proteins; D009841:Oligonucleotides