Characterization of human cyclooxygenase 2 gene promoter localization of a TGF-beta response element.
We characterized 2.3 kb of 5'-flanking region of the human cyclooxygenase 2 gene and analyzed its promoter activity. We localized three positive and two negative cis-acting regulatory regions and a TGF-beta response region. Basal promoter activity was located between -77 and +99 bp. Maximal promoter activity was observed from -1838 to +99 bp. A TGF-beta response region was found between -454 and -288 bp. This region included an NF-I site. Electrophoretic mobility shift assays showed that TGF-beta caused a marked change in the pattern and intensity of this NF-I like protein complex.
Yang
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X
Department of Biochemistry, Boston University School of Medicine, MA 02118, USA.
Hou
F
F
Taylor
L
L
Polgar
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P
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D051546:Cyclooxygenase 2; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008168:Lung / Q000201:enzymology; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D007527:Isoenzymes; D008565:Membrane Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D004247:DNA; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases