title: Rhamnogalacturonan II, a dominant polysaccharide in juices produced by enzymic liquefaction of fruits and vegetables.
abstract: Rhamnogalacturonan II (RG-II), a small complex pectic polysaccharide, is released from apple (Malus domestica), carrot (Daucus carota), and tomato (Solanum lycopersicum) by treatment with two commercial liquefying enzyme preparations. RG-II was isolated by size-exclusion chromatography from apple, tomato, and carrot juices obtained by enzymic liquefaction. All the RG-IIs contained the diagnostic sugars, apiose, 2-O-methyl-L-fucose, 2-O-methyl-D-xylose, aceric acid, Kdo and Dha. Glycosyl-linkage compositions of the neutral and acidic sugars, including aceric acid, were consistent with the hypothetical model described for sycamore RG-II confirming the conservation of RG-II in plants. Thus, when pectinolytic enzyme preparations are used to process fruits and vegetables, RG-II is released as a main soluble polysaccharide fraction while other pectic polysaccharides are heavily degraded.
authors.affiliation: Institut National de la Recherche Agronomique, Unité de Recherches des Polymères et des Techniques Physico-Chimiques, Montpellier, France.
authors.forename: T
authors.initials: T
authors.lastname: Doco
authors.forename: P
authors.initials: P
authors.lastname: Williams
authors.forename: S
authors.initials: S
authors.lastname: Vidal
authors.forename: P
authors.initials: P
authors.lastname: Pellerin
mesh_terms: D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D018552:Daucus carota / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D018551:Solanum lycopersicum / Q000737:chemistry*; D009005:Monosaccharides / Q000032:analysis; D010368:Pectins / Q000737:chemistry* / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism
chemical_list: D009005:Monosaccharides; C042492:rhamnogalacturonan II; D010368:Pectins; D011096:Polygalacturonase