Comparative effects of pravastatin-induced DNA synthesis on serum-stimulated synchronized and proliferative rat mesangial cells.
To compare the effects of pravastatin, a 3-hydroxy-3-methylglutaryl-Coenzyme A (HMG-CoA) reductase inhibitor, on DNA synthesis in synchronized and proliferative rat glomerular mesangial cells (MCs), growth-arrested and proliferative MCs were exposed to medium containing 20% or 10% FBS, respectively, to induce mitogenesis. Different doses of pravastatin (10(-3)-10(-9) M) were added for 48 h. DNA synthesis was assessed by Bromodeoxyuridine (BrdU) incorporation. Pravastatin caused a significant (P < 0.05) dose-dependent reduction in BrdU incorporation in both serum-stimulated synchronized and proliferative MCs, but higher doses are required in the latter.
Higueruelo
S
S
Nephrology Research Unit, Hospital Universitario Germans Trias i Pujol, Badalona, Spain.
Romero
R
R
D000818:Animals; D000963:Antimetabolites; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008297:Male; D017035:Pravastatin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000963:Antimetabolites; D004791:Enzyme Inhibitors; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D004247:DNA; D001973:Bromodeoxyuridine; D017035:Pravastatin