Effects of interleukin-6 on in vitro cell attachment, migration and invasion of human ovarian carcinoma.
High levels of IL-6 have been observed in ascitic fluid in patients with ovarian cancer. In this study, we examined the effects of IL-6 on in vitro invasiveness of human ovarian cancer cells. Human ovarian cancer cells, NOM1 and SKOV, were used. Cell attachment to Matrigel, migration, and invasion were examined with or without IL-6. Zymography was performed to analyze gelatinase production by these cells. NOM1 cell attachment was increased by IL-6 (0 to 10 ng/ml). In a migration assay, IL-6 ranging from 0 to 10 ng/ml showed chemotactic and/or chemokinetic activities for the two cell lines. The invasiveness of these cells was significantly enhanced by IL-6 at the above concentrations. However, IL-6 did not modulate the production of either MMPs or TIMPs. IL-6 affects cell invasion through its effects on attachment and migration in human ovarian cancer cells. IL-6 might thus contribute to the progression of this disease.
Obata
N H
NH
Department of Gynecology and Obstetrics, Nagoya University School of Medicine, Japan.
Tamakoshi
K
K
Shibata
K
K
Kikkawa
F
F
Tomoda
Y
Y
D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen; D004338:Drug Combinations; D005260:Female; D018093:Gelatinases / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D007797:Laminin; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D014176:Protein Biosynthesis; D011509:Proteoglycans; D019716:Tissue Inhibitor of Metalloproteinase-2; D019714:Tissue Inhibitor of Metalloproteinases
D004338:Drug Combinations; D006023:Glycoproteins; D015850:Interleukin-6; D007797:Laminin; D011509:Proteoglycans; D019714:Tissue Inhibitor of Metalloproteinases; C059485:matrigel; D019716:Tissue Inhibitor of Metalloproteinase-2; D003094:Collagen; D018093:Gelatinases