Blockage of muscle and neuronal nicotinic acetylcholine receptors by fluoxetine (Prozac).
Fluoxetine (Prozac), a widely used antidepressant, is said to exert its medicinal effects almost exclusively by blocking the serotonin uptake systems. The present study shows that both muscle and neuronal nicotinic acetylcholine receptors are blocked, in a noncompetitive and voltage-dependent way, by fluoxetine, which also increases the rate of desensitization of the nicotinic receptors. Because these receptors are very widely distributed in the both central and peripheral nervous systems, the blocking action of fluoxetine on nicotinic receptors may play an important role in its antidepressant and other therapeutical effects. Our findings will help to understand the mode of action of fluoxetine, and they may also help to develop more specific medicinal drugs.
García-Colunga
J
J
Department of Psychobiology, University of California, Irvine 92697-4550, USA.
Awad
J N
JN
Miledi
R
R
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000494:pharmacology*; D005473:Fluoxetine / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects; D008564:Membrane Potentials; D051379:Mice; D009132:Muscles / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D009865:Oocytes; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology; D014981:Xenopus
D018687:Antidepressive Agents, Second-Generation; D007473:Ion Channels; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D005473:Fluoxetine; D012701:Serotonin; D000109:Acetylcholine