Molecular beacons: trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria.
Molecular beacons are fluorescent probes developed for solution rather than membrane hybridization. We have investigated the utility of these probes to study rumen microbial ecology. Two cellulolytic species, Ruminococcus albus and Fibrobacter succinogenes, were tested. Membrane and solution hybridizations gave similar results in competition experiments with cocultures of R. albus 8 and F. succinogenes S85.
Schofield
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Department of Animal Science, Cornell University, Ithaca, New York 14853, USA.
Pell
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Krause
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D000818:Animals; D001419:Bacteria / Q000235:genetics*; D004463:Ecology; D005456:Fluorescent Dyes; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012417:Rumen / Q000382:microbiology*; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D012336:RNA, Ribosomal, 16S