title: Subconductance states of a mutant NMDA receptor channel kinetics, calcium, and voltage dependence.
abstract: The kinetic properties of main and subconductance states of a mutant mouse N-methyl-D-aspartate (NMDA) receptor channel were examined. Recombinant receptors made of zeta-epsilon 2 (NR1-NR2B) subunits having asparagine-to-glutamine mutations in the M2 segment (zeta N598Q/epsilon 2N589Q) were expressed in Xenopus oocytes. Single channel currents recorded from outside-out patches were analyzed using hidden Markov model techniques. In Ca(2+)-free solutions, an open receptor channel occupies a main conductance (93 pS) and a subconductance (62 pS) with about equal probability. There are both brief and long-lived subconductance states, but only a single main level state. At -80 mV, the lifetime of the main and the longer-lived sub level are both approximately 3.3 ms. The gating of the pore and the transition between conductance levels are essentially independent processes. Surprisingly, hyperpolarization speeds both the sub-to-main and main-to-sub transition rate constants (approximately 120 mV/e-fold change), but does not alter the equilibrium occupancies. Extracellular Ca2+ does not influence the transition rate constants. We conclude that the subconductance levels arise from fluctuations in the energetics of ion permeation through a single pore, and that the voltage dependence of these fluctuations reflects the modulation by the membrane potential of the barrier between the main and subconductance conformations of the pore.
authors.affiliation: Department of Biophysical Sciences, State University of New York at Buffalo 14214, USA.
authors.forename: L S
authors.initials: LS
authors.lastname: Premkumar
authors.forename: F
authors.initials: F
authors.lastname: Qin
authors.forename: A
authors.initials: A
authors.lastname: Auerbach
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D004594:Electrophysiology; D015640:Ion Channel Gating / Q000502:physiology; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008390:Markov Chains; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008818:Mice, Neurologic Mutants; D008954:Models, Biological; D009154:Mutation / Q000502:physiology*; D018408:Patch-Clamp Techniques; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics* / Q000378:metabolism*; D012815:Signal Processing, Computer-Assisted
chemical_list: D007473:Ion Channels; D016194:Receptors, N-Methyl-D-Aspartate; D002118:Calcium