Stroke-prone SHR and arteriolipidosis-prone SHR as models for atherosclerosis: their mechanisms and application for nutritional and pharmacological studies.
1. Peritoneal macrophages (Mphi) were harvested from non-stimulated 2 month old SHRSP and WKY, and incubated with acetyl LDL or [14C]-oleate-albumin complex to check total, free and esterified cholesterol (Cho) accumulation as well as acy coenzyme A-cholesterol acyltransferase (ACAT) activity and its activation by hyperlipidaemic serum. 2. Total and esterified Cho accumulation as well as ACAT activity were enhanced in Mphi from SHRSP compared with those from WKY. 3. To observe the dietary or pharmacological influence on atherogenesis, arteriolipidosis-prone SHR (ALR) were given a high fat cholesterol diet with 3% taurine or a Ca blocker (Nilvadipine, N; 30 mg/kg), both of which significantly decreased serum Cho level and clearly attenuated arterial fat deposition.
Yamori
Y
Y
World Health Organization Collaborating Centre for Research on Primary Prevention of Cardiovascular Diseases, Life Science, Kyoto University, Japan.
Murakami
S
S
Nara
Y
Y
Ikeda
K
K
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001161:Arteriosclerosis / Q000188:drug therapy / Q000503:physiopathology*; D002561:Cerebrovascular Disorders / Q000235:genetics / Q000503:physiopathology*; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood; D004195:Disease Models, Animal; D066298:In Vitro Techniques; D008064:Lipidoses / Q000235:genetics / Q000503:physiopathology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D002788:Cholesterol Esters; D002784:Cholesterol