title: Manipulating photosynthesis.
abstract: The levels of individual photosynthetic proteins can be independently decreased by the Agrobacterium-mediated transformation of plants with antisense RNA constructs. Protocols for the introduction of such constructs into Agrobacterium, the Agrobacterium-mediated transformation of tobacco leaf disks, and the screening and analysis of the transgenic plants produced are described.
authors.affiliation: School of Biological Sciences, University of Manchester, UK. Jacqui.Knight@man.ac.i.uk
authors.forename: J S
authors.initials: JS
authors.lastname: Knight
authors.forename: F
authors.initials: F
authors.lastname: Madueño
authors.forename: S A
authors.initials: SA
authors.lastname: Barnes
authors.forename: J C
authors.initials: JC
authors.lastname: Gray
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D005822:Genetic Vectors; D010788:Photosynthesis; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D016372:RNA, Antisense; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D016372:RNA, Antisense