Changing course of growing axons in the optic chiasm of the mouse.
The distribution of retinofugal fibres has been studied by electron microscopy throughout the extent of the developing mouse optic nerve and chiasm at embryonic day (E) 16, in order to determine the course of fibre growth. Growth cones and mature axons, which are randomly distributed in bundles in the extracranial optic nerve, segregate in the juxtachiasmatic optic nerve. Here, growth cones accumulate in subpial regions amongst the endfeet of radial glia, whereas axons lie in the depths of the nerve. Surprisingly, however, growth cones move away from this region toward the ventricular zone in the lateral and midline parts of the chiasm, only to return to subpial regions once more before entering the optic tract, where fibres are again in an age-related order. Superficially, mature axons mingle with growth cones in the chiasm and near the beginning of the optic tract, suggesting that the age-related order begins to be reestablished before growth cones enter the tract. Deep and superficial regions of the pathway were examined in different planes of section. Specialised membrane relationships between retinofugal fibres and radial glial cells were also studied in deep and superficial regions of the lateral part of the chiasm. In addition, the distribution of retinofugal fibre bundles in the adult mouse was looked at by using light microscopy. The changing fibre positions noted in the embryo are maintained in the adult.
Colello
S J
SJ
Department of Human Anatomy, University of Oxford, United Kingdom.
Coleman
L A
LA
D000818:Animals; D001369:Axons / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009412:Nerve Fibers / Q000502:physiology* / Q000648:ultrastructure; D009457:Neuroglia / Q000166:cytology; D009897:Optic Chiasm / Q000196:embryology / Q000648:ultrastructure*; D009900:Optic Nerve / Q000196:embryology / Q000648:ultrastructure*