Molecular cloning and expression of a novel Staphylococcus aureus antigen.
A novel gene encoding an antigen from Staphylococcus aureus was isolated from an expression library by screening with antisera from patients with deep Staphylococcus aureus infections. In one positive clone an open reading frame, named ORF-2, was identified. Recombinant ORF-2 protein reacted with human immune serum. ORF-2 was shown to be present in other Staphylococcus aureus strains, but not in related species.
Rieneck
K
K
Institute for Inflammation Research, Laboratory of Medical Immunology, TA 7544, Rigshospitalet, Copenhagen, N. Denmark. rieneck@biobase.dk
Renneberg
J
J
Diamant
M
M
Gutschik
E
E
Bendtzen
K
K
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013203:Staphylococcal Infections / Q000276:immunology; D013211:Staphylococcus aureus / Q000235:genetics* / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D011994:Recombinant Proteins