title: A new high molecular weight agglutinin from garlic (Allium sativum).
abstract: Erythrocyte agglutination by lectins from Allium sativum was inhibited only by mannose of the sugars tested. However, asialofetuin was more effective inhibitor of agglutination as compared to mannose. This led to the use of an asialofetuin-silica affinity column to isolate agglutinins of 110 and 25 kDa (ASA110 and ASA25). While ASA25 is a dimeric protein comprising of subunits of 12.5 and 13.0 kDa, ASA110 is a glycoprotein of two identical subunits of 47 kDa. ASA110 revealed to have a high content of aspartic acid, glycine, leucine and serine but low content of cysteine and methionine. It contains 14 residues of neutral sugars in addition to 43 residues of hexosamines per mole of lectin and requires metal ions for its functional conformation. Serological cross-reactions with other species showed some common epitopes of ASA110 and ASA25 present in A. porrum, A. ascalonicum, Narcissus alba, PHA and Con A but not in A. cepa. ASA110 with CHO cells indicated it to be weakly cytotoxic with LD50 of 160 microg/ml.
authors.affiliation: Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, India.
authors.forename: A
authors.initials: A
authors.lastname: Gupta
authors.forename: R S
authors.initials: RS
authors.lastname: Sandhu
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001212:Asialoglycoproteins; D002846:Chromatography, Affinity; D060748:Fetuins; D005737:Garlic / Q000737:chemistry*; D006168:Guinea Pigs; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000302:isolation & purification*; D008970:Molecular Weight; D037121:Plant Lectins; D010946:Plants, Medicinal; D011487:Protein Conformation; D011817:Rabbits; D012822:Silicon Dioxide; D000509:alpha-Fetoproteins
chemical_list: D000596:Amino Acids; D001212:Asialoglycoproteins; D060748:Fetuins; D037102:Lectins; D037121:Plant Lectins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D012822:Silicon Dioxide