The presence of GPI-linked protein(s) in an archaeobacterium, Sulfolobus acidocaldarius, closely related to eukaryotes.
GPI-anchored proteins are distributed ubiquitously in eukaryotes, but not in procaryotes. By metabolic-labeling of Sulfolobus acidocaldarius cells, 14C-radiolabeled precursors of GPI and caldarchaetidylinositol were incorporated into 120, 143 and 185 kDa proteins. The 185 kDa protein was specifically solubilized by bacterial phosphatidylinositol-specific phospholipase C. Therefore, Sulfolobus proved to contain at least one GPI-anchored proteins.
Kobayashi
T
T
Department of Microbial Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan.
Nishizaki
R
R
Ikezawa
H
H
D019143:Evolution, Molecular; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D010802:Phylogeny; D011506:Proteins / Q000737:chemistry*; D016945:Sulfolobus acidocaldarius / Q000737:chemistry*
D017261:Glycosylphosphatidylinositols; D011506:Proteins