Complete coding sequence, exon/intron arrangement and chromosome location of ZNF45, a KRAB-domain-containing gene.
Zinc finger genes represent a large multigene family present in mammalian and other genomes. A subgroup of these genes contain a conserved motif, the KRAB domain, at the NH2-terminal region, which was recently shown to posses a potent transcriptional repression activity. For one such gene, ZNF45, we determined the complete coding sequence, along with the exon/intron arrangement and the splice junctions. Exon 2 codes exclusively for the KRAB-A element of 42 amino acids, and exon 4 contains the 19 zinc finger repeats. In addition, we generated an EST (expressed sequence tag) from the 3'-end of ZNF45 that we used for polymerase chain reaction screening of a P1-derived genomic library. We isolated a 65-kb clone that was used for localizing this gene on chromosome 19q13.2 by fluorescence in situ hybridization.
Constantinou Deltas
C
C
The Cyprus Institute of Neurology and Genetics, Nicosia. deltasco@mdrtc.cing.ac.cy
Bashiardes
E
E
Patsalis
P C
PC
Hadjimarcou
M
M
Kroisel
P M
PM
Ioannou
P A
PA
Roses
A D
AD
Lee
J E
JE
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D016384:Consensus Sequence; D005091:Exons / Q000235:genetics; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D051741:Kruppel-Like Transcription Factors; D008969:Molecular Sequence Data; D005810:Multigene Family; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics*; D016415:Sequence Alignment
D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; C105105:ZNF45 protein, human