title: Increased incidence of disomic sperm nuclei in a 47,XYY male assessed by fluorescent in situ hybridization (FISH).
abstract: Using triple-colour fluorescent in situ hybridization in decondensed sperm heads, we assessed the sex-chromosome distribution in spermatozoa from a 47,XYY male compared with controls. The incidence of spermatozoa with 24,XY (0.30%) and 24,YY (1.01%) disomy was significantly higher than in our control series. Diploid meiocytes present in the ejaculate were mainly 47,XYY (60.6-86.7%), and haploid meiocytes were mainly 24,XY (78.1%).These results suggest that, although the extra Y chromosome is thought to be eliminated during spermatogenesis, XYY germ cells can complete meiosis and produce disomic spermatozoa.
authors.affiliation: Unitat de Biologia Cellular, Facultat de Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Blanco
authors.forename: C
authors.initials: C
authors.lastname: Rubio
authors.forename: C
authors.initials: C
authors.lastname: Simon
authors.forename: J
authors.initials: J
authors.lastname: Egozcue
authors.forename: F
authors.initials: F
authors.lastname: Vidal
mesh_terms: D000328:Adult; D000782:Aneuploidy; D002467:Cell Nucleus / Q000648:ultrastructure; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008540:Meiosis; D013094:Spermatozoa / Q000166:cytology* / Q000648:ultrastructure; D013194:Staining and Labeling; D014997:XYY Karyotype