Application of capillary gas chromatography to the study of hydrolysis of the nerve agent VX in rat plasma.
We present here a gas chromatography technique allowing the detection and quantification of VX [O-ethyl S-(2-diisopropylaminoethyl)methylphosphonothiolate] as well as its P-S bond hydrolysis product diisopropylaminoethanethiol directly from spiked rat plasma. This technique was applied to study VX hydrolysis in rat plasma. We observed that 53 +/- 4% of 374 microM VX disappeared from spiked plasma after 2 h. VX disappearance was mainly related to enzymatic cleavage of the P-S bond (Km = 2.5 mM and Vmax = 13.3 nmol min-1 ml-1 of rat plasma). The activity was totally inhibited by 1 mM Hg2+ and was also inhibited by metal chelators.
Bonierbale
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Laboratoire d'Analyses Toxicologiques, Centre d'Etudes du Bouchet, Vert-Le-Petit, France.
Debordes
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Coppet
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D000818:Animals; D043303:Aryldialkylphosphatase; D002614:Chelating Agents / Q000494:pharmacology; D002619:Chemical Warfare Agents / Q000032:analysis*; D002800:Cholinesterase Inhibitors / Q000097:blood; D002849:Chromatography, Gas; D004950:Esterases / Q000378:metabolism; D005021:Ethylamines / Q000097:blood; D006868:Hydrolysis; D008297:Male; D008628:Mercury / Q000494:pharmacology; D009946:Organothiophosphorus Compounds / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000097:blood
D043303:Aryldialkylphosphatase; D002614:Chelating Agents; D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D004950:Esterases; D005021:Ethylamines; D008628:Mercury; D009946:Organothiophosphorus Compounds; D013438:Sulfhydryl Compounds; C105867:2-(N,N-diisopropylamino)ethanethiol; C009680:VX-agent