title: Compact tunable Cr:LiSAF laser for infrared matrix-assisted laser desorption/ionization.
abstract: A tunable Cr:LiSAF laser-pumped optical parametric oscillator was used for mid-infrared matrix-assisted laser desorption/ionization (MALDI) mass spectrometry experiments. The mass spectra of substance P, bovine insulin and pd(T)10 in the 2.88-2.96 microns range showed excellent single shot signal quality (signal-to-noise-ratio, resolution) but poor shot-to-shot reproducibility. The reproducibility is expected to improve with more stable laser design. No correlation was found between the absorption spectrum of the matrix and the MALDI response.
authors.affiliation: Department of Chemistry, George Washington University, Washington, D.C. 20052, USA.
authors.forename: M
authors.initials: M
authors.lastname: Sadeghi
authors.forename: Z
authors.initials: Z
authors.lastname: Olumee
authors.forename: X
authors.initials: X
authors.lastname: Tang
authors.forename: A
authors.initials: A
authors.lastname: Vertes
authors.forename: Z X
authors.initials: ZX
authors.lastname: Jiang
authors.forename: A J
authors.initials: AJ
authors.lastname: Henderson
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: C R
authors.initials: CR
authors.lastname: Prasad
mesh_terms: D000818:Animals; D002417:Cattle; D007259:Infrared Rays; D007328:Insulin / Q000032:analysis; D007834:Lasers; D009696:Nucleic Acids / Q000032:analysis; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation*; D013055:Spectrophotometry, Infrared; D013373:Substance P / Q000032:analysis
chemical_list: D007328:Insulin; D009696:Nucleic Acids; D013373:Substance P