Mannoproteins from Candida albicans elicit a Th-type-1 cytokine profile in human Candida specific long-term T cell cultures.
Since T lymphocytes are active producers of regulatory cytokines, long-term T cell cultures (LTTC) specific for a major mannoproteic antigen (MP) of Candida albicans from peripheral blood mononuclear cells (PBMC) of healthy donors were generated and their cytokine profile studied. LTTC consisted of an expanded CD3CD4 T-helper (Th) populations, with a high proportion of CD45R0 T memory cells. Stimulation of LTTC by MP induced a Th-1 type cytokine profile, as indicated by the presence of IFN-gamma and the absence of IL-5 (both as mRNA and protein) in cell cultures and supernatants. These results suggest a predominant Th1 response elicited by C. albicans mannoprotein antigen in human PBMC.
La Sala
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Department of Bacteriology, Istituto Superiore di Sanità, Roma, Italy.
Urbani
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Torosantucci
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Cassone
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Ausiello
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CM
D000946:Antigens, Fungal / Q000276:immunology* / Q000302:isolation & purification; D002176:Candida albicans / Q000737:chemistry / Q000276:immunology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007376:Interleukin-2 / Q000494:pharmacology; D017493:Leukocyte Common Antigens / Q000032:analysis; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D008775:Methylprednisolone / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology
D000946:Antigens, Fungal; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; C025363:mannoproteins; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens; D008775:Methylprednisolone