Application of fluorescence in situ hybridization to detect residual leukemic cells with 9;22 and 15;17 translocations.
We performed fluorescence in situ hybridization (FISH) upon 9;22 and 15;17 translocation-positive bone marrow cells to monitor the clinical course of 46 patients with chronic myelocytic leukemia (CML) and nine with acute promyelocytic leukemia (AML M3) who received chemotherapy and/or bone marrow transplantation (BMT). M-BCR-ABL and PML-RAR alpha probes were used to detect translocations of t(9;22) and t(15;17), respectively. Signals from CML patients treated with interferon (17 patients) or BMT (29 patients) were 0.5-15% positive for the 9;22 translocation. Among nine M3 patients who received extensive chemotherapy or BMT, 1-5% were positive for the 15;17 translocation. A highly sensitive FISH procedure using both translocation probes and a whole chromosome Y probe was established and applied to eight sex-mismatched BMT patients (seven CML and one AML M3), in which 0.1-0.6% of signals positive for the specific translocations were detected. These results suggested that interphase FISH is powerful enough to identify minor cell populations of 9;22 or 15;17 translocations after therapy, as well as to detect specific chromosome abnormalities at diagnosis.
Tanaka
K
K
Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Minami-ku, Japan.
Arif
M
M
Eguchi
M
M
Kumaravel
T S
TS
Ueda
R
R
Ohno
R
R
Iwato
K
K
Kyo
T
T
Dohy
H
H
Kamada
N
N
D001853:Bone Marrow / Q000648:ultrastructure; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D015342:DNA Probes; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis / Q000235:genetics*; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis / Q000235:genetics*; D018613:Microscopy, Confocal; D018365:Neoplasm, Residual; D009504:Neutrophils / Q000648:ultrastructure; D014178:Translocation, Genetic
D015342:DNA Probes; D016044:Fusion Proteins, bcr-abl