title: Development of an ELISA for maduramicin and determination of the depletion kinetics of maduramicin residues in poultry.
abstract: Polyclonal antibodies against the coccidiostat maduramicin were produced by the immunization of rabbits with a conjugate between maduramicin and human serum albumin. The antibodies exhibited minimal cross-reactivity with structurally similar ionophoric coccidiostats (monensin, salinomycin, narasin and laslocid). An enzyme-linked immunosorbent assay (ELISA) was developed for maduramicin in poultry tissue using these antibodies. The ELISA had a limit of detection of approximately 0.02 ng/g in tissue (ng/ml in serum) and a limit of determination of 1.0 ng/g in tissue (ng/ml in serum). The half-life of maduramicin was measured in broiler chickens that had been treated with a therapeutic dose of maduramicin for 14 days. Following withdrawal of the medicated feed, the mean half-lives of maduramicin were 20, 39 and 13 h in liver, muscle and serum, respectively. At the recommended withdrawal period (5 days) the mean residual maduramicin concentration in liver was 1.6 ng/g, in muscle was 1.5 ng/g and in serum was 0.05 ng/ml.
authors.affiliation: Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, UK.
authors.forename: D G
authors.initials: DG
authors.lastname: Kennedy
authors.forename: W J
authors.initials: WJ
authors.lastname: Blanchflower
authors.forename: B C
authors.initials: BC
authors.lastname: O'Dornan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D002645:Chickens / Q000378:metabolism*; D004350:Drug Residues / Q000032:analysis* / Q000493:pharmacokinetics; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D006207:Half-Life; D006801:Humans; D007783:Lactones / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D007783:Lactones; C018091:maduramicin