title: T-2 tetraol is cytotoxic to a chicken macrophage cell line.
abstract: Cytotoxic effects of T-2 tetraol, a T-2 toxin derivative, on the MQ-NCSU chicken macrophage cell line were quantified by direct in vitro exposure. Macrophage cultures were exposed to 1, 10, 20, 40, 80, 160, and 320 micrograms/mL of T-2 tetraol for 1 h. Macrophage viability after exposure to T-2 tetraol. Macrophage viability was reduced by increasing concentrations of T-2 tetraol (linear effect, P < or = 0.001; quadratic effect, P < or = 0.025). The ability of macrophages to adhere to glass surfaces was impaired by increasing concentrations of T-2 tetraol (linear effect, P < or = 0.003). This experiment demonstrates that T-2 tetraol is cytotoxic to chicken macrophages in vitro.
authors.affiliation: Department of Poultry Science, North Carolina State University, Raleigh 27695-7608, USA.
authors.forename: M T
authors.initials: MT
authors.lastname: Kidd
authors.forename: M A
authors.initials: MA
authors.lastname: Qureshi
authors.forename: W M
authors.initials: WM
authors.lastname: Hagler
authors.forename: R
authors.initials: R
authors.lastname: Ali
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D008264:Macrophages / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000633:toxicity
chemical_list: C046700:T-2 tetraol; D013605:T-2 Toxin