Cooperative inhibition of acetylcholinesterase activities by hexachlorophene in human erythrocytes.
Hexachlorophene (HCP), pentachlorophenol (PCP), 2,4,5-trichlorophenol (2,4,5-TCP) and 2,4,6-trichlorophenol (2,4,6-TCP) all hemolyzed washed human erythrocytes and inhibited acetylcholinesterase (AchE) activities in erythrocyte membrane. HCP was much more potent in either effect than any other compound examined. The inhibition of AchE activities by HCP was reversed on adding albumin. The dose-response curves by HCP and PCP were sigmoidal, indicating cooperative inhibition, while those by 2,4,5-TCP and 2,4,6-TCP were not. Furthermore, the cooperativity of the inhibition by HCP was greater than by PCP. Differing from that by PCP, the cooperativity of inhibition increased depending on the temperature (13, 25, 37 degrees C) and decreased when the membrane was treated with Triton X-100. The cooperativity was also decreased in the presence of albumin. On a Scatchard plot analysis, erythrocyte membranes appeared to have multiple binding sites of different affinities for HCP; binding of HCP to the low affinity site [dissociation constant (Kd) 4.7 x 10(-5) M] seemed to be responsible for the observed cooperative inhibition of AchE activities. Neither neostigmine nor fenitrothion altered the cooperativity. HCP seems to be the most potent cooperative inhibitor of AchE in human erythrocyte membranes known to date. HCP may be useful to examine AchE and milieu in human erythrocyte membranes.
Matsumura
H
H
Department of Environmental Toxicology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Matsuoka
M
M
Igisu
H
H
Ikeda
M
M
D000328:Adult; D002733:Chlorophenols / Q000097:blood / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000097:blood / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D006461:Hemolysis / Q000187:drug effects; D006582:Hexachlorophene / Q000097:blood / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D013696:Temperature
D002733:Chlorophenols; D002800:Cholinesterase Inhibitors; D006582:Hexachlorophene