title: Oronasal leishmaniasis caused by a parasite with an unusual isoenzyme profile.
abstract: A 45-year-old Sudanese man from western Sudan presented with oronasal leishmaniasis of three years duration. He had no history of previous kala-azar or cutaneous leishmaniasis. The parasite isolated from the oral mucosa was characterized by isoenzymes using 12 enzymes and by polymerase chain reaction amplification of kinetoplast DNA using species-specific primers. The specific primers gave products indistinguishable from those of the Leishmania donovani complex. However, the isoenzyme profile showed a zymodeme pattern that was significantly different from the zymodemes previously reported in the Sudan and the Ethiopian region.
authors.affiliation: Institute of Endemic Diseases, University of Khartoum, Sudan.
authors.forename: M
authors.initials: M
authors.lastname: Ibrahim
authors.forename: A
authors.initials: A
authors.lastname: Suliman
authors.forename: F A
authors.initials: FA
authors.lastname: Hashim
authors.forename: A
authors.initials: A
authors.lastname: Khalil el-T
authors.forename: D A
authors.initials: DA
authors.lastname: Evans
authors.forename: A
authors.initials: A
authors.lastname: Kharazmi
authors.forename: A M
authors.initials: AM
authors.lastname: el Hassan
mesh_terms: D000818:Animals; D017931:DNA Primers; D018105:DNA, Kinetoplast / Q000032:analysis; D004592:Electrophoresis, Starch Gel; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007891:Leishmania / Q000145:classification / Q000201:enzymology*; D007893:Leishmania donovani / Q000145:classification / Q000235:genetics; D007896:Leishmaniasis / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000469:parasitology* / Q000473:pathology; D009297:Nasal Mucosa / Q000469:parasitology / Q000473:pathology; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013397:Sudan
chemical_list: D017931:DNA Primers; D018105:DNA, Kinetoplast; D007527:Isoenzymes