title: [The production of HIV recombinant proteins and their immunochemical characteristics].
abstract: Several Escherichia coli strains producing HIV-specific recombinant proteins including env-1, env-2, and gag sequences have been obtained using different gene engineering techniques. These proteins have been isolated and purified from bacterial lysates by ion exchange chromatography on DEAE-Toyopearl and Streamline-SP columns. Polyacrylamide gel electrophoresis and immunoblotting have been used to prove recombinant proteins purity and to identify them. These proteins have been shown to be adequate as antigen preparations for enzyme immunoassay test-systems aimed at anti-HIV antibodies detection.
authors.forename: D L
authors.initials: DL
authors.lastname: Martynenko
authors.forename: T A
authors.initials: TA
authors.lastname: Serhieieva
authors.forename: R M
authors.initials: RM
authors.lastname: Chumak
authors.forename: N V
authors.initials: NV
authors.lastname: Ivans'ka
authors.forename: M P
authors.initials: MP
authors.lastname: Zavelevych
mesh_terms: D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D015497:HIV-1 / Q000378:metabolism*; D015498:HIV-2 / Q000378:metabolism*; D015151:Immunoblotting; D007120:Immunochemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D014764:Viral Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D014764:Viral Proteins
vernacular_title: Oderzhannia rekombinatnykh bilkiv VIL ta ïkh imunokhimichna kharakterystyka.