title: Identification and analysis of a lytic-phase origin of DNA replication in human herpesvirus 7.
abstract: Human herpesvirus 7 (HHV-7) DNA sequences colinear with the HHV-6 lytic-phase origin of DNA replication (oriLyt) were amplified by PCR. Plasmid constructs containing these sequences were replicated in HHV-7-infected cord blood mononuclear cells but not in HHV-6-infected cells. In contrast, plasmids bearing HHV-6 oriLyt were replicated in both HHV-6- and HHV-7-infected cells. Finally, the minimal HHV-7 DNA element necessary for replicator activity was mapped to a 600-bp region which contains two sites with high homology to the consensus binding site for the HHV-6 origin binding protein. At least one of these binding sites was shown to be essential for replicator function of HHV-7 oriLyt.
authors.affiliation: Department of Microbiology and Immunology, Medical Center, University of Rochester, New York 14642, USA.
authors.forename: N
authors.initials: N
authors.lastname: van Loon
authors.forename: C
authors.initials: C
authors.lastname: Dykes
authors.forename: H
authors.initials: H
authors.lastname: Deng
authors.forename: G
authors.initials: G
authors.lastname: Dominguez
authors.forename: J
authors.initials: J
authors.lastname: Nicholas
authors.forename: S
authors.initials: S
authors.lastname: Dewhurst
mesh_terms: D001483:Base Sequence; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000502:physiology; D016199:Herpesvirus 7, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D018741:Replication Origin; D014779:Virus Replication
chemical_list: D004279:DNA, Viral