In vitro activities of tetracycline & ciprofloxacin against Chlamydia trachomatis isolates from conjunctivitis patients.
Twenty seven Chlamydia trachomatis isolates from patients of conjunctivitis were tested for their in vitro sensitivities to ciprofloxacin and tetracycline in cyclohexamide treated McCoy cells on cover slip (shell vial) cultures. After a 48 h exposure of chlamydia infected monolayers to varying concentrations of each of the drugs, the cover slips were processed and stained for detection of major out membrane protein of C. trachomatis by fluorescent antibody test (FAT) using fluorescein conjugated monoclonal. Minimum inhibitory concentration (MIC90) and minimum lethal concentration (MLC90) of ciprofloxacin were 2.9 micrograms/ml and 5.7 micrograms/ml and for tetracycline 9.1 micrograms/ml and 18.0 micrograms/ml respectively. Ciprofloxacin may have a more promising role in treating chlamydial conjunctivitis than the commonly used tetracycline.
Madhavan
H N
HN
Vision Research Foundation, Madras.
Roy
S
S
Malathy
J
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002692:Chlamydia trachomatis / Q000187:drug effects*; D002939:Ciprofloxacin / Q000494:pharmacology*; D003231:Conjunctivitis / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D013752:Tetracycline / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D002939:Ciprofloxacin; D013752:Tetracycline