title: ADAM 13: a novel ADAM expressed in somitic mesoderm and neural crest cells during Xenopus laevis development.
abstract: Embryonic development involves a series of cell adhesive interactions that provide mechanical and instructive information required for morphogenesis. The ADAMs family of membrane-anchored proteins, containing a disintegrin and metalloprotease domain, is well suited for participating in such developmental events. They encode not only a potential adhesive function, through an integrin-binding disintegrin domain, but also a potential antiadhesive function, through a zinc-dependent metalloprotease domain. In order to investigate the role of ADAMs in early development we cloned a cDNA encoding a novel member of the ADAM family from a Xenopus laevis neurula stage library. We call this cDNA, and the 915-amino-acid protein it encodes, ADAM 13, X-ADAM 13 RNA is expressed during embryogenesis from the midblastula stage through tadpole stage 45. X-ADAM 13 is localized to somitic mesoderm and cranial neural crest cells during gastrulation, neurulation, and in tail bud stages. Sequence analyses of the X-ADAM 13 metalloprotease and disintegrin domains indicate that the protein is likely to be involved in both proteolytic and cell-adhesive functions. The X-ADAM 13 sequence is most closely related to that of mouse meltrin alpha, which is implicated in myoblast fusion. Our data suggest that X-ADAM 13 may be involved in neural crest cell adhesion and migration as well as myoblast differentiation.
authors.affiliation: Department of Cell Biology, Health Sciences Center, University of Virginia, Charlottesville 22908, USA.
authors.forename: D
authors.initials: D
authors.lastname: Alfandari
authors.forename: T G
authors.initials: TG
authors.lastname: Wolfsberg
authors.forename: J M
authors.initials: JM
authors.lastname: White
authors.forename: D W
authors.initials: DW
authors.lastname: DeSimone
mesh_terms: D016475:3T3 Cells; D051722:ADAM Proteins; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019483:Disintegrins / Q000235:genetics / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000378:metabolism; D006801:Humans; D008322:Mammals; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D008666:Metalloendopeptidases / Q000235:genetics / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D019170:Somites / Q000378:metabolism*; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology
chemical_list: D018076:DNA, Complementary; D019483:Disintegrins; D008565:Membrane Proteins; D012333:RNA, Messenger; D029867:Xenopus Proteins; D051722:ADAM Proteins; C104937:ADAM33 protein, Xenopus; D008666:Metalloendopeptidases