Paediatric rhabdomyosarcoma: MyoD1 demonstration in routinely processed tissue sections using wet heat pretreatment (pressure cooking) for antigen retrieval.
OBJECTIVE
To investigate wet heat pretreatment (pressure cooking) as a means of antigen retrieval for demonstration of MyoD1 in paraffin wax embedded tissue.


METHODS
Routinely processed tissue sections of transmission electron microscope confirmed cases of rhabdomyosarcoma were stained immunohistochemically with the MyoD1 antibody. Antigen retrieval was achieved by wet heat pretreatment of the tissue sections.


RESULTS
MyoD1 was stained successfully in all seven cases. The protein was localised to nuclei and cytoplasm depending on the type of tumour cell.


CONCLUSIONS
Wet heat pretreatment for antigen retrieval from routinely processed tissue sections permits excellent subsequent immunostaining for MyoD1 in rhabdomyoblasts.
Engel
M E
ME
Department of Pathology, Red Cross Children's Hospital, Rondebosch, South Africa.
Mouton
S C
SC
Emms
M
M
D016591:Histocytological Preparation Techniques; D006801:Humans; D007150:Immunohistochemistry; D017570:MyoD Protein / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D012208:Rhabdomyosarcoma / Q000378:metabolism*
D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D009363:Neoplasm Proteins