title: Deletion of Src homology 3 domain results in constitutive activation of Tec protein-tyrosine kinase.
abstract: Tec protein-tyrosine kinase (PTK) is the prototype of a new subfamily of non-receptor type PTKs, and is abundantly expressed in hematopoietic tissues. We have revealed that Tec is inducibly tyrosine-phosphorylated and activated by stimulation with a wide range of cytokines. To get more insight into the signaling mechanism through Tec, we have generated a constitutively active form of Tec PTK. Deletion of the Src homology (SH) 3 domain gave rise to a hyperphosphorylated and activated Tec kinase (Tec deltaSH3). The activity of Tec deltaSH3 was confirmed in 293 cells, as well as in cytokine-dependent hematopoietic cells (BA/F3). Tec deltaSH3 should be a useful tool to study the in vivo substrates of Tec PTK.
authors.affiliation: Department of Molecular Biology, Jichi Medical School, Kawachi-gun, Tochigi.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: A
authors.initials: A
authors.lastname: Miyazato
authors.forename: K
authors.initials: K
authors.lastname: Ohya
authors.forename: U
authors.initials: U
authors.lastname: Ikeda
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Ozawa
authors.forename: H
authors.initials: H
authors.lastname: Mano
mesh_terms: D000818:Animals; D001665:Binding Sites; D004789:Enzyme Activation; D017353:Gene Deletion; D051379:Mice; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D018909:src Homology Domains
chemical_list: C089848:Tec protein-tyrosine kinase; D011505:Protein-Tyrosine Kinases