Aspergillus nidulans maintains short telomeres throughout development.
We report the identification and cloning of the telomeres of the filamentous fungus,Aspergillus nidulans. We have identified three classes of cloned chromosomal ends based on the telomere-associated sequences (TASs) and demonstrated that the telomeric repeat sequence is TTAGGG, identical to that found in vertebrates, including humans, and some lower eukaryotes. One category of telomere clones was found to contain internal, variant TAAGGG repeats. The A.nidulans telomeric tract length is strikingly short (4-22 repeats). We demonstrate that telomere length is remarkably stable in different cell types and at altered growth temperatures, suggesting a highly regulated mechanism for length control.
Bhattacharyya
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Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0414, USA. anamitra@cgl.ucsf.edu
Blackburn
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EH
D001233:Aspergillus nidulans / Q000254:growth & development*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry; D004706:Endodeoxyribonucleases / Q000378:metabolism; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000235:genetics*; D013696:Temperature
D004271:DNA, Fungal; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31