TCR V alpha chain expression influences reactivity to the hapten TNP.
We have recently demonstrated a remarkable selection of in vitro cultivated, TNP-specific polyclonal T cell lines for the expression of a TCR beta chain encoded by the V beta 8.2 gene. The goal of the present study was to analyse V alpha usage in V beta 8.2 T cells responsive to TNP, using TNP-specific T cell lines derived from three common strains of mice, as well as from V beta 8.2 transgenic mice. Results indicate that in vitro TNP stimulation of T cells from TNP-immune mice results in significant skewing of V alpha usage among responding V beta 8.2+ T cells, with overexpression observed for V alpha 3.2 and V alpha 8. These results indicate that V alpha expression influences recognition of TNP by T cells, and suggest that the hapten TNP might be recognized like typical peptide antigens by combinatorial TCR alpha and beta contact sites.
Dieli
F
F
Institute of General Pathology, University of Palermo, Italy.
Asherson
G L
GL
Tomonari
K
K
Sireci
G
G
Caccamo
N
N
Salerno
A
A
D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D005810:Multigene Family / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014301:Trinitrobenzenes / Q000276:immunology*
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006241:Haptens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014301:Trinitrobenzenes