Effect of pre-induction of heat shock proteins on indomethacin-induced small-intestinal lesion in rats.
Systemic hyperthermia induces the synthesis of heat shock proteins (HSPs) in several organs. However, the mechanism of induction and the functions of HSPs in the small-intestinal mucosa have not been established. We examined the expression of HSPs in the small-intestinal mucosa after systemic hyperthermia, and evaluated the cytoprotective function of pre-induced HSPs on experimentally induced mucosal damage. HSP expression was investigated by Western blot and densitometric analysis before and after hyperthermia (42.5 degrees C; 20 min). Expression of a 72-kDa heat shock protein (HSP72) and a 73-kDa heat shock protein (HSP73), both of which are endogenous cytoprotectants in vitro significantly increased, peaking 6-9 h after hyperthermia, without any pathologic alterations, whereas the expression of a 60-kDa heat shock protein (HSP60) did not increase. To investigate the influence of pre-induction of HSPs on small-intestinal damage, rats received indomethacin (10 mg/kg; orally) with or without pre-treatment with hyperthermia. Small-intestinal damage caused by indomethacin was not influenced by pre-induction of HSP72 and HSP73. We demonstrated that systemic hyperthermia induced HSP72 and HSP73, although pre-induction of these proteins did not have a cytoprotective function in the small-intestinal damage caused by indomethacin.
Jin
M
M
First Department of Internal Medicine, Akita University School of Medicine, Japan.
Otaka
M
M
Otani
S
S
Okuyama
A
A
Itoh
S
S
Iwabuchi
A
A
Sasahara
H
H
Fujimori
S
S
Itoh
H
H
Tashima
Y
Y
Masamune
O
O
D000818:Animals; D005334:Fever / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000502:physiology; D007213:Indomethacin / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D006360:Heat-Shock Proteins; D007213:Indomethacin