Thermodynamic analysis of antigen-antibody binding using biosensor measurements at different temperatures.
The thermodynamic parameters of the interaction between hen egg white lysozyme and Fab D1.3 were determined by measuring the temperature dependence of the ratio of its kinetic association and dissociation rate constants. Biosensor technology (BIAcore 2000) was used to measure the rate constants at temperatures ranging from 5 to 40 degrees C. The value of DeltaG degrees at 25 degrees C (-49 kJ M-1) calculated by this method was very close to that obtained previously from fluorescence quenching measurements (-48.5 kJ M-1). However, the value of DeltaH degrees measured at 25 degrees C by biosensor technology (-35 kJ M-1) was smaller than that determined previously by microcalorimetry (-90 kJ M-1). Another difference was the limited variation of ln K and DeltaG with temperature observed with BIAcore compared to the steady decrease of ln K with temperature found by calorimetry. Our data showed that the binding reaction was driven only by enthalpy below 23 degrees C, by enthalpy and entropy between 23 and 35 degrees C, and only by entropy above 35 degrees C. This suggests, inter alia, that the contribution from the enthalpy of hydration due to the water molecules present at the interface in the lysozyme-antibody complex is progressively eliminated as the temperature increases. Whereas calorimetric data pertain to all the components present in the sample, including solvent molecules, BIAcore measurements monitor only the physical association and dissociation of the two macromolecular species. The difference between the two sets of data may also reflect the complexity of the binding mechanism between lysozyme and Fab D1.3.
Zeder-Lutz
G
G
Immunochemistry Laboratory, UPR 9021 CNRS, IBMC 15 rue René Descartes, Strasbourg, 67084, France.
Zuber
E
E
Witz
J
J
Van Regenmortel
M H
MH
D000818:Animals; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D002151:Calorimetry; D002645:Chickens; D007140:Immunoglobulin Fab Fragments; D008956:Models, Chemical; D013816:Thermodynamics
D007140:Immunoglobulin Fab Fragments