title: In vivo and in vitro studies on the stereoselective hydrolysis of tri- and diglycerides by gastric and pancreatic lipases.
abstract: The stereoselectivity of dog gastric and dog pancreatic lipases was investigated both in vitro, under simulated physiological conditions, and in vivo, during the digestion of a liquid test meal. In vitro it was observed that although both lipases had a stereopreference for the sn-3 position in triglycerides, it was about three times higher in the case of the gastric lipase. On the other hand, both lipases clearly showed a comparable enantioselectivity for the sn-1 position when a racemic diolein was used as the substrate. In the case of pancreatic lipase, the enantiomeric excess of 1,2-sn-diolein generated in vitro by the hydrolysis of triolein was found to decrease significantly, and even to be slightly reversed, at high rates of hydrolysis (above 50%) due to the further stereoselective hydrolysis of diglycerides into monoglycerides. This finding may explain the low enantiomeric excess of the diglycerides observed in vivo during the early phase of intraduodenal digestion when pancreatic lipase plays a predominant role and the rate of triolein hydrolysis is already high. On the other hand, a large enantiomeric excess of 1,2-sn-diolein generated from triolein was always the fingerprint of the gastric lipase in vitro even at high hydrolysis rates. This fingerprinting of gastric lipase was observed during both the intragastric phase and the late intestinal phase of lipolysis. This feature was therefore taken as an index to determine the respective roles of gastric and pancreatic lipases during in vivo lipolysis. To the best of our knowledge, this is the first time that stereoselectivity has been used as a tool to discriminate between the activities of two enzymes hydrolyzing the same substrate in vivo.
authors.affiliation: Laboratoire de Lipolyse Enzymatique UPR 9025-CNRS. 31, Marseille, France.
authors.forename: F
authors.initials: F
authors.lastname: Carrière
authors.forename: E
authors.initials: E
authors.lastname: Rogalska
authors.forename: C
authors.initials: C
authors.lastname: Cudrey
authors.forename: F
authors.initials: F
authors.lastname: Ferrato
authors.forename: R
authors.initials: R
authors.lastname: Laugier
authors.forename: R
authors.initials: R
authors.lastname: Verger
mesh_terms: D000818:Animals; D004075:Diglycerides / Q000378:metabolism*; D004285:Dogs; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D013237:Stereoisomerism; D013270:Stomach / Q000201:enzymology*; D013379:Substrate Specificity; D014304:Triolein / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D014304:Triolein; D008049:Lipase; C013965:diolein