Effect of capsianoside, a diterpene glycoside, on tight-junctional permeability.
Previous work (Hashimoto et al., (1994) Biosci. Biotech. Biochem. 58, 1345) revealed that a sweet pepper extract enhanced the tight-junctional (TJ) permeability of a human intestinal Caco-2 cell monolayer. In the present study, the substance which modulated the TJ permeability was chromatographically purified from the extract. The active substances were identified as capsianosides A-F, diterpene glycosides. Treatment of the cells with capsianoside F, the most active compound, decreased the cellular G-actin content by 40% and increased the F-actin content by 16%. The effect of capsianoside F was significantly suppressed by disturbing the cytoskeletal structure with cytochalasin D at a low dose (50 ng/ml). These results suggest that capsianosides affected the cytoskeletal function by modulating the reorganization of actin filaments, by which the TJ structure and permeability were changed. The possible involvement of a PKC inhibition in the mechanism of an increase in TJ permeability is also suggested.
Hashimoto
K
K
School of Food and Nutritional Sciences, University of Shizuoka, Japan. hashimot@fnsl.u-shizuoka-ken.ac.jp
Kawagishi
H
H
Nakayama
T
T
Shimizu
M
M
D000199:Actins / Q000032:analysis; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002614:Chelating Agents; D003599:Cytoskeleton / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry; D011060:Poloxalene / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D019108:Tight Junctions / Q000187:drug effects*; D014675:Vegetables / Q000737:chemistry
D000199:Actins; D002614:Chelating Agents; D004224:Diterpenes; D006027:Glycosides; D010936:Plant Extracts; D013501:Surface-Active Agents; D011060:Poloxalene; D011493:Protein Kinase C