Evidence that the carboxy groups of Asp158 in papain and caricain have abnormally low pKa values and thus do not contribute the key ionisations with pKa 4 that generate catalytic competence.
Noble
M
M
Department of Biochemistry, Queen Mary & Westfield College, University of London, UK.
Abramson
D
D
Verma
C
C
Brocklehurst
K
K
D001224:Aspartic Acid; D001665:Binding Sites; D002384:Catalysis; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D010206:Papain / Q000737:chemistry* / Q000378:metabolism*; D010940:Plant Proteins; D017354:Point Mutation; D011487:Protein Conformation; D013053:Spectrophotometry
D010940:Plant Proteins; D001224:Aspartic Acid; D003546:Cysteine Endopeptidases; D010206:Papain; C039974:caricain