[Sequence analysis of the carboxy terminal of the Epstein-Barr virus LMP-1 oncogene in Epstein-Barr virus associated disorders].
The LMP-1 oncogene (BNLF-1 gene) of Epstein-Barr virus(EBV) is the only EBV protein with known oncogenic potential. The presence of 30-base pair(bp) deletion mutants within the carboxy terminal end of the LMP-1 oncogene of EBV has been reported in EBV-associated neoplasms. We analysed the 30-bp deletion and the single-base mutations of the LMP-1 gene in 13 spontaneously established lymphoblastoid cell lines (LCLs) from peripheral blood mononuclear cells of 3 healthy children, 4 patients with EBV-unrelated acute febrile illnesses, 3 patients with infectious mononucleosis (IM), and 3 patients with chronic active EBV infection (CEBV), and 6 frozen samples from 4 patients with CEBV and 2 patients with EBV-associated hemophagocytic syndrome (EBV-AHS). For molecular analysis of the carboxy terminal end of the LMP-1 gene, PCR was performed using primers spanning the carboxy terminal region of the LMP-1 gene. Direct sequence analysis of the PCR products revealed identical 30-bp deletion in 14 of 19 samples (74%). Six point mutations at nucleotide positions 168357, 168355, 168320, 168308, 168295, and 168225 were frequently identified regardless of disease atatus. Our findings revealed the carboxy terminal end of the LMP-1 gene was mutational hot spots. The 30-bp deletion mutant is widely spread in the Japanese population and is not implicated in EBV-associated lymphoproliferative diseases.
Itakura
O
O
Department of Pediatrics, Hokkaido University School of Medicine.
Kikuta
H
H
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006566:Herpesviridae Infections / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D017421:Sequence Analysis; D014412:Tumor Virus Infections / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D009856:Oncogene Proteins, Viral; D014763:Viral Matrix Proteins