title: Detection of antibodies against porcine parvovirus nonstructural protein NS1 may distinguish between vaccinated and infected pigs.
abstract: The humoral antibody response against the nonstructural protein NS1 and the structural protein VP2 of porcine parvovirus (PPV) was evaluated by immuno-peroxidase test (IPT) and enzyme linked immuno sorbent assay (ELISA) using recombinant PPV antigens. The coding sequence for NS1 and VP2 was inserted into the baculovirus. Autographa californica nuclear polyhedrosis virus (AcNPV) genome resulting in two recombinant baculoviruses AcNPV-NS1 and AcNPV-VP2, respectively. Sf9 cells (Spodoptora frugidiperda) inoculated with AcNPV-NS1 producing recombinant nonstructural protein (rNS1) and AcNPV-VP2 producing recombinant virion protein (rVP2) were used in IPT and ELISA to analyse serum antibodies. Pigs vaccinated with an inactivated whole virus vaccine and experimentally infected pigs were studied. Significant titers against rVP2 were obtained in both vaccinated and infected pigs. Specific antibodies against rNS1 could only be detected in infected pigs and NS1 may in this way allow the specific detection of infected animals. Analysis of serum samples collected up to 18 days post infection (p.i.) from four pigs experimentally infected with PPV showed that antibodies against rNS1 and rVP2 could in all cases be detected on day 9 p.i. Two individual pigs were inoculated twice with PPV and the antibody response was followed 89 days after second inoculation. Serum antibodies against both rVP2 and rNS1 could be detected for this period of time.
authors.affiliation: Danish Veterinary Institute For Virus Research, Kalvehave, Denmark. esm@sviv.lfm.dk
authors.forename: E S
authors.initials: ES
authors.lastname: Madsen
authors.forename: K G
authors.initials: KG
authors.lastname: Madsen
authors.forename: J
authors.initials: J
authors.lastname: Nielsen
authors.forename: M H
authors.initials: MH
authors.lastname: Jensen
authors.forename: J C
authors.initials: JC
authors.lastname: Lei
authors.forename: P
authors.initials: P
authors.lastname: Have
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D001483:Base Sequence; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D010322:Parvoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D017992:Parvovirus / Q000276:immunology* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D018411:Spodoptera; D013552:Swine; D013553:Swine Diseases; D014162:Transfection; D014611:Vaccination / Q000662:veterinary*; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000276:immunology*; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; C106202:NS1 protein, parvovirus; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines