Immunohistochemical study of hepatocellular carcinoma-specific aldehyde dehydrogenase.
Tumor-associated aldehyde dehydrogenase (ALDH) was reported in cases of human hepatocellular carcinoma and animal hepatoma models. This ALDH isozyme is similar to ALDH3 which exists in the stomach and lung; however, the biochemical and clinical significance of this unique ALDH isozyme have not been established. Human tumor-associated ALDH was purified, and polyclonal antibodies prepared. Using these antibodies, specific development of tumor-associated ALDH was confirmed by immunohistochemical techniques. It was found that about 50% of hepatocellular carcinomas reacted with the antibody. This unique ALDH isozyme may be a novel tumor marker of hepatocellular carcinoma.
Shibuya
A
A
Department of Internal Medicine, Kitasato University, School of Medicine, Sagamihara, Kanagawa, Japan.
Takeuchi
A
A
Shibata
H
H
Saigenji
K
K
Yoshida
A
A
D000444:Aldehyde Dehydrogenase / Q000276:immunology*; D000906:Antibodies; D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D046508:Culture Techniques; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000276:immunology*; D008113:Liver Neoplasms / Q000276:immunology*
D000906:Antibodies; D014408:Biomarkers, Tumor; D000444:Aldehyde Dehydrogenase