Effect of a retroviral immunodeficiency syndrome on murine cytomegalovirus-induced hepatitis.
We have studied the effects of LP-BM5 MuLV-induced murine acquired immunodeficiency syndrome (MAIDS) on concomitant murine cytomegalovirus (MCMV) infection in the livers of C57BL mice. A delayed inflammatory response in livers of mice with MAIDS (M+) on day 4 was associated with impaired clearance of MCMV-infected cells 6 days after infection. This correlated with increased levels of inflammation and serum alanine transaminase. The latter reflects enhanced hepatic necrosis, which was evident histologically. Delayed-type hypersensitivity responses to MCMV antigen were unimpaired in M+ mice and were mediated by CD8+ cells. Depletion of NK1.1+ cells from M+ mice increased MCMV replication and associated liver damage on day 6, whereas CD8+ depletion had little effect. In contrast, in the presence of CD8+ cells M- C57BL mice did not require NK1.1+ cells for control of hepatic MCMV infection, but dual NK1.1+ and CD8+ depletion dramatically potentiated hepatic MCMV replication. Our results suggest that M+ mice may acquire non-NK1.1+ and non-CD8+ cells that are able to partially control hepatic MCMV infection. These findings are discussed with reference to mortality in M+ mice after high-dose MCMV infection, as this is initially delayed but ultimately higher than in M- controls.
Peacock
C D
CD
Department of Microbiology, University of Western Australia, Nedlands, Australia.
Olver
S D
SD
Price
P
P
D000818:Animals; D018414:CD8-Positive T-Lymphocytes; D002452:Cell Count; D005260:Female; D006524:Hepatitis, Viral, Animal / Q000150:complications / Q000473:pathology* / Q000821:virology; D006566:Herpesviridae Infections / Q000150:complications / Q000473:pathology*; D006968:Hypersensitivity, Delayed; D007694:Killer Cells, Natural / Q000502:physiology; D008099:Liver / Q000473:pathology / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000473:pathology; D018146:Muromegalovirus / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D015996:Survival Rate; D014779:Virus Replication