title: Molecular cloning and primary characterization of a single-chain antibody against human sarcoma-associated antigen p200.
abstract: BACKGROUND
  Monoclonal Antibody (MAb) 29-13 reacts with the human sarcoma-associated antigen (SAA) p200. We report here engineering and primary characterization of a single chain antibody (scFV2913).
  
  
  METHODS
  The scFV2913 recombinant gene, consisting of VH-linker-VK, was constructed with RT-PCR. This gene was cloned and expressed in E. coli. The renatured scFV2913 was used in the immunostaining study.
  
  
  RESULTS
  Consistent with its parent MAb 29-13, purified and renatured scFV2913 showed affinity and specificity to the SAA p200 according to the immuno-histochemical staining study of 99 specimens of human sarcomas and other tissues.
  
  
  CONCLUSIONS
  Due to its retained specificity and affinity, scFV2913 may be useful in immunodiagnosis and immunotherapy for sarcoma patients.
authors.affiliation: Department of Surgical Oncology, University of Illinois College of Medicine at Chicago, USA.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: J J
authors.initials: JJ
authors.lastname: Stastny
authors.forename: A
authors.initials: A
authors.lastname: Shilkaitis
authors.forename: X
authors.initials: X
authors.lastname: Liao
authors.forename: T K
authors.initials: TK
authors.lastname: Das Gupta
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D012509:Sarcoma / Q000276:immunology*; D057127:Single-Chain Antibodies
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; C100570:antigen p200; C104655:recombinant antibody scFV2913