title: Studies on stannous fluoride toothpaste and gel (1). Antimicrobial properties and staining potential in vitro.
abstract: Stannous fluoride (SF) in a toothpaste vehicle has the potential to provide anticaries and plaque inhibitory benefits through the fluoride and antimicrobial stannous moieties respectively. Dental staining, however, can occur by precipitation of dietary chromogens onto the tooth surface by stannous ions. These studies in vitro compare the antimicrobial profile and propensity to cause tea staining of a number of stannous fluoride formulations. The formulations used were 2 SF toothpaste products (SF1, SF2), 2 experimental SF plus stannous pyrophosphate toothpastes (SFSP1, SFSP2), a SF gel (G) and a NaF toothpaste (C). Maximum inhibitory dilution values against a range of oral bacteria were determined by agar dilution. Tea staining was measured spectrophotometrically on saliva coated clear acrylic blocks exposed to slurries of the paste or gel. All formulations showed antimicrobial activity with the order of greatest activity downwards being C, SF2, SF1, SFSP1, SFSP2 and G. Tea staining at 10 exposures was in the following descending order of optical density SFSP1, SFSP2, G. C, SF1, SF2, water control. The antimicrobial profile of G was similar to that of SF, whereas that of the other formulations were varied but similar to a detergent profile. The difference in staining suggested considerable variation in availability of stannous ions in the formulations. However, the propensity for stannous ions to stain must be balanced against the stain removal propensity of the contained detergents in the toothpaste formulations. In conclusion, the variation in antimicrobial activity and more particularly staining activity of the formulations suggest the products will vary in activity in vivo.
authors.affiliation: Division of, Restorative Dentistry, Dental School, University of Bristol, England.
authors.forename: W
authors.initials: W
authors.lastname: Wade
authors.forename: M
authors.initials: M
authors.lastname: Addy
authors.forename: J
authors.initials: J
authors.lastname: Hughes
authors.forename: S
authors.initials: S
authors.lastname: Milsom
authors.forename: F
authors.initials: F
authors.lastname: Doherty
mesh_terms: D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology*; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D018565:Food-Drug Interactions; D005782:Gels; D008826:Microbial Sensitivity Tests; D013662:Tea / Q000009:adverse effects / Q000737:chemistry; D014002:Tin Fluorides / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D014003:Tin Polyphosphates / Q000737:chemistry / Q000494:pharmacology; D014075:Tooth Discoloration / Q000139:chemically induced*; D014100:Toothpastes
chemical_list: D000891:Anti-Infective Agents, Local; D005782:Gels; D013662:Tea; D014002:Tin Fluorides; D014003:Tin Polyphosphates; D014100:Toothpastes