Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients.
A plasma PCR test, using a nonradioactive PCR plate assay, was evaluated for detection of human cytomegalovirus reactivation. This assay was compared to Southern blotting and found to perform well. As a noncompetitive method of quantitation, it was similar to a competitive method for detecting the number of genome copies per milliliter of plasma in marrow transplant recipients. This is a technically simplified assay with potential for adaptation to automation.
Gallez-Hawkins
G M
GM
Department of Pediatrics, City of Hope National Medical Center, Duarte, California 91010, USA.
Tegtmeier
B R
BR
ter Veer
A
A
Niland
J C
JC
Forman
S J
SJ
Zaia
J A
JA
D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000209:etiology / Q000821:virology*; D015342:DNA Probes / Q000235:genetics; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014773:Virology / Q000379:methods
D015342:DNA Probes; D004279:DNA, Viral