Eotaxin is a potent chemotaxin for human basophils.
We studied the effect of eotaxin, a novel eosinophil-active CC chemokine with high target cell specificity, on human basophils. Eotaxin induced higher levels of chemotactic response with a lower ED50 compared with RANTES in basophils; half-maximal migration occurred at a concentration of approximately 3 nM. On the other hand, it exerted only a marginal effect on either histamine release or leukotriene C4 generation. In addition, nested PCR amplification experiments revealed the expression of CC CKR3, a putative receptor for eotaxin, on basophils. Since accumulation of both basophils and eosinophils is an important aspect of allergic inflammation, eotaxin potentially plays a pathogenic role in allergic disorders by inducing migration of both of these cell types.
Yamada
H
H
Department of Medicine and Physical Therapy, University of Tokyo School of Medicine, Japan.
Hirai
K
K
Miyamasu
M
M
Iikura
M
M
Misaki
Y
Y
Shoji
S
S
Takaishi
T
T
Kasahara
T
T
Morita
Y
Y
Ito
K
K
D001491:Basophils / Q000166:cytology / Q000187:drug effects*; D054413:Chemokine CCL11; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil / Q000494:pharmacology*; D016207:Cytokines / Q000494:pharmacology*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
C515456:CCL11 protein, human; D054413:Chemokine CCL11; D018925:Chemokines; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil; D016207:Cytokines; D012333:RNA, Messenger