Cloning of a cDNA that encodes farnesyl diphosphate synthase and the blue-light-induced expression of the corresponding gene in the leaves of rice plants.
A cDNA encoding farnesyl diphosphate synthase (FPPS), a key enzyme in isoprenoid biosynthesis, was isolated from a cDNA library constructed from mRNA that had been prepared from etiolated rice (Oriza sativa L. variety Nipponbare) seedlings after three hours of illumination by a subtraction method. The putative polypeptide deduced from the 1289 bp nucleotide sequence consisted of 353 amino acids and had a molecular mass of 40 676 Da. The predicted amino acid sequence exhibited high homology to those of FPPS from Arabidopsis (73% to type 1, 72% to type 2) and white lupin (74%). Southern blot analysis showed that the rice genome might contain only one gene for FPPS. The highest level of expression of the gene was demonstrated in leaves by RNA blot analysis. Moreover, light, in particular blue light, effectively enhanced expression of the gene.
Sanmiya
K
K
BioScience Center, Nagoya University, Japan.
Iwasaki
T
T
Matsuoka
M
M
Miyao
M
M
Yamamoto
N
N
D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D017343:Genes, Plant / Q000235:genetics*; D051228:Geranyltranstransferase; D008027:Light; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D014166:Transferases / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051228:Geranyltranstransferase