title: N,N-dimethylsphingosine phosphorylation in human platelets.
abstract: Metabolism of sphingosine (Sph) derivatives in human platelets was examined. [3H]Sph was rapidly and heavily phosphorylated into sphingosine 1-phosphate, similarly in resting and stimulated platelets. [14C]N,N-dimethylsphingosine was stable in resting platelets, while it was converted into N,N-dimethylsphingosine 1-phosphate (DMS-1-P), although weakly, in platelets stimulated with thrombin or 12-O-tetradecanoylphorbol 13-acetate. This DMS-1-P formation was inhibited by staurosporine, a potent protein kinase inhibitor. [3H]C2-ceramide was unchanged both in resting and stimulated platelets. Our report is the first to describe production of DMS-1-P in a biological system.
authors.affiliation: Department of Laboratory Medicine, Yamanashi Medical University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yatomi
authors.forename: Y
authors.initials: Y
authors.lastname: Ozaki
authors.forename: K
authors.initials: K
authors.lastname: Satoh
authors.forename: S
authors.initials: S
authors.lastname: Kume
authors.forename: F
authors.initials: F
authors.lastname: Ruan
authors.forename: Y
authors.initials: Y
authors.lastname: Igarashi
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D002518:Ceramides / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D015539:Platelet Activation; D015398:Signal Transduction; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D019311:Staurosporine / Q000494:pharmacology
chemical_list: D002518:Ceramides; C105100:N,N-dimethylsphingosine-1-phosphate; D019311:Staurosporine; C061800:N,N-dimethylsphingosine; D013110:Sphingosine