title: Thrombolysis-induced coronary reperfusion causes acute and massive interstitial release of cardiac muscle cell proteins.
abstract: OBJECTIVE
  Reperfusion of the infarct-related artery in patients with acute myocardial infarction limits infarct size, but also causes accelerated release into plasma of cardiac tissue proteins. The latter effect could reflect either enhanced protein washout from the heart or abrupt disruption of myocyte membranes. The present study indicates that the latter mechanism prevails.
  
  
  METHODS
  In 26 patients, patency of the infarct-related artery was determined by coronary angiography 90 min and 5-7 days after thrombolytic treatment. Continuous electrocardiography was performed during the first 24 h after admission. Cumulative release of myoglobin (Mb) and creatine kinase (CK) into plasma was calculated from frequently sampled plasma concentrations.
  
  
  RESULTS
  In patients with a patent infarct-related artery after 90 min, onset of a rapid (> 50%) decrease in ST-vector magnitude coincided with an equally rapid increase in QRS-vector magnitude, and with a sudden onset of release into plasma of Mb as well as CK. In these patients, a maximal initial release rate was observed and cumulative release conformed closely to a simple model for sudden interstitial liberation of proteins. In contrast, protein release started more gradually and could not be fitted to this model, in patients with persistent occlusion of the infarct-related artery at 90 min and absence of ST-vector normalisation.
  
  
  CONCLUSIONS
  Previous studies have demonstrated significant myocardial salvage by timely reperfusion therapy. Nevertheless, this study indicates that the moment of recanalisation of the infarct-related artery coincides with sudden and massive disruption of myocyte membranes. Attenuation of this effect, if possible, could further improve the benefits of reperfusion therapy.
authors.affiliation: Thorax Center, University Hospital Rotterdam, Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Cobbaert
authors.forename: W T
authors.initials: WT
authors.lastname: Hermens
authors.forename: P P
authors.initials: PP
authors.lastname: Kint
authors.forename: P J
authors.initials: PJ
authors.lastname: Klootwijk
authors.forename: F
authors.initials: F
authors.lastname: Van de Werf
authors.forename: M L
authors.initials: ML
authors.lastname: Simoons
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017023:Coronary Angiography; D003402:Creatine Kinase / Q000097:blood*; D015716:Electrocardiography, Ambulatory; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000097:blood; D009211:Myoglobin / Q000097:blood*; D012044:Regression Analysis; D015912:Thrombolytic Therapy; D013997:Time Factors
chemical_list: D009211:Myoglobin; D003402:Creatine Kinase