Direct interaction of the mitochondrial membrane protein carnitine palmitoyltransferase I with Bcl-2.
To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in coimmunoprecipitations using two different kinds of anti-Bcl-2 antibodies.
Paumen
M B
MB
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
Ishida
Y
Y
Han
H
H
Muramatsu
M
M
Eguchi
Y
Y
Tsujimoto
Y
Y
Honjo
T
T
D000818:Animals; D017209:Apoptosis; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship
D008565:Membrane Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D002334:Carnitine O-Palmitoyltransferase