Identification of human and mouse GP-1, a putative member of a novel G-protein family.
To identify genes induced in monocytes by interferon-gamma, we carried out PCR-based cDNA subtraction and subsequent differential display on mRNA isolated from a human monocytic leukemia cell line, THP-1. We detected a novel gene encoding a protein bearing GTP-binding motifs, the characteristics of GTP-binding proteins (G-proteins). We also identified the mouse homologue of this gene and designated the gene GP-1. The amino acid sequence of GP-1 deduced from the nucleotide sequence is highly conserved in human and mouse (97% identical over the entire protein), suggesting a fundamental physiological role for this molecule. As amino acid sequences of GTP-binding motifs of human and mouse GP-1 are practically identical to those of recently identified putative G-proteins of nematode, AGP-1 and CGP-1, these proteins are likely to be members of the same, novel G-protein family. GP-1 mRNA was readily detected in mouse brain, thymus, lung, and kidney, while GP-1 mRNA is rarely expressed in liver.
Senju
S
S
Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Sciences, Japan.
Nishimura
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary; D019204:GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; C484499:Gtpbp1 protein, mouse; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; C105125:GTPBP1 protein, human; D020559:Monomeric GTP-Binding Proteins