Synthetic study of phosphopeptides related to heat shock protein HSP27.
Two kinds of phosphoserine-containing peptides related to HSP27 were synthesized by the Boc- or Fmoc-mode solid-phase method based on prephosphorylation strategy. In the case of the Boc strategy, the O-phosphono group of the phosphoserine residue was protected with the cyclopentyl or cyclohexyl group. On the other hand, N'-Fmoc-O-[(benzyloxy)-hydroxyphosphinyl]serine was employed in case of the Fmoc strategy. Consequently, it has become-feasible to utilize conventional solid-phase methods for synthesizing any phosphopeptides which are required to elucidate biochemical significance of protein phosphorylation.
Wakamiya
T
T
Department of Chemistry, Faculty of Science and Technology, Kinki University, Osaka, Japan.
Togashi
R
R
Nishida
T
T
Saruta
K
K
Yasuoka
J
J
Kusumoto
S
S
Aimoto
S
S
Kumagaye
K Y
KY
Nakajima
K
K
Nagata
K
K
D002851:Chromatography, High Pressure Liquid; D006360:Heat-Shock Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010748:Phosphopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment
D006360:Heat-Shock Proteins; D010748:Phosphopeptides