OCI-5/rat glypican-3 binds to fibroblast growth factor-2 but not to insulin-like growth factor-2.
OCI-5 encodes the rat homologue of glypican-3, a membrane-bound heparan sulfate proteoglycan that is mutated in the Simpson-Golabi-Behmel overgrowth syndrome. OCI-5 and glypican-3 are 95% identical. It has been recently suggested that glypican-3 interacts with insulin-like growth factor-2 (IGF-2) and that this interaction regulates IGF-2 activity. We report here that we have transfected OCI-5 into two different cell lines, and we have not been able to detect an interaction between the OCI-5 proteoglycan produced by the transfected cells and IGF-2. On the other hand, we have found that OCI-5 interacts with FGF-2, as has already been shown for glypican-1. This interaction is mediated by the heparan sulfate chains of OCI-5 because it can be inhibited by heparin or by heparitinase.
Song
H H
HH
Department of Medical Biophysics, University of Toronto, Toronto, Ontario M4N 3M5, Canada.
Shi
W
W
Filmus
J
J
D000818:Animals; D002460:Cell Line; D017077:Culture Media, Conditioned; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D053673:Glypicans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D011485:Protein Binding; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014162:Transfection
D017077:Culture Media, Conditioned; D016222:Fibroblast Growth Factor 2; D053673:Glypicans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D007335:Insulin-Like Growth Factor II; D011509:Proteoglycans