title: Intragenic complementation at the human argininosuccinate lyase locus. Identification of the major complementing alleles.
abstract: To determine the molecular and biochemical basis of intragenic complementation observed at the human argininosuccinate lyase (ASL) locus, we identified the ASL alleles in ASL-deficient cell strains with two unique complementation phenotypes: (i) frequent complementers, strains that participated in the majority of complementation events, and (ii) high activity complementers, strains in which complementation was associated with a relatively high level of restoration of ASL activity. Four mutations (Q286R, D87G, A398D, and a deletion of exon 13) were identified in the four strains examined. One of the two frequent complementers was homozygous, and the other heterozygous, for the Q286R allele. Similarly, one of the two high activity complementers was homozygous, and the other heterozygous, for the D87G allele. When the Q286R and D87G mutations were introduced by site-directed mutagenesis into wild-type ASL cDNA, each conferred loss of ASL activity in COS cell transfection assays. To test directly the hypothesis that intragenic complementation occurs at the ASL locus, one of the major complementation events observed previously, between strains carrying the Q286R and D87G alleles, was reconstructed in COS cell transfection assays. A partial restoration of ASL activity, comparable with the increase seen in the fibroblast complementation analysis, was observed on joint cotransfection of these two alleles. The results provide molecular confirmation of the major features of the ASL mutant complementation map, identify the Q286R and D87D alleles as the frequent and high activity complementing alleles, respectively, and provide direct proof of intragenic complementation at the ASL locus.
authors.affiliation: Department of Genetics, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada.
authors.forename: D C
authors.initials: DC
authors.lastname: Walker
authors.forename: J
authors.initials: J
authors.lastname: Christodoulou
authors.forename: H J
authors.initials: HJ
authors.lastname: Craig
authors.forename: L R
authors.initials: LR
authors.lastname: Simard
authors.forename: L
authors.initials: L
authors.lastname: Ploder
authors.forename: P L
authors.initials: PL
authors.lastname: Howell
authors.forename: R R
authors.initials: RR
authors.lastname: McInnes
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001123:Argininosuccinate Lyase / Q000235:genetics*; D056807:Argininosuccinic Aciduria; D019556:COS Cells; D005816:Genetic Complementation Test; D006579:Heterozygote; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
chemical_list: D001123:Argininosuccinate Lyase