title: SBF cell cycle regulator as a target of the yeast PKC-MAP kinase pathway.
abstract: Protein kinase C (PKC) signaling is highly conserved among eukaryotes and has been implicated in the regulation of cellular processes such as cell proliferation and growth. In the budding yeast, PKC1 functions to activate the SLT2(MPK1) mitogen-activated protein (MAP) kinase cascade, which is required for the maintenance of cell integrity during asymmetric cell growth. Genetic studies, coimmunoprecipitation experiments, and analysis of protein phosphorylation in vivo and in vitro indicate that the SBF transcription factor (composed of Swi4p and Swi6p), an important regulator of gene expression at the G1 to S phase cell cycle transition, is a target of the Slt2p(Mpk1p) MAP kinase. These studies provide evidence for a direct role of the PKC1 pathway in the regulation of the yeast cell cycle and cell growth and indicate that conserved signaling pathways can act to control key regulators of cell division.
authors.affiliation: Department of Biology, Yale University, Post Office Box 208103, New Haven, CT 06520-8103, USA.
authors.forename: K
authors.initials: K
authors.lastname: Madden
authors.forename: Y J
authors.initials: YJ
authors.lastname: Sheu
authors.forename: K
authors.initials: K
authors.lastname: Baetz
authors.forename: B
authors.initials: B
authors.lastname: Andrews
authors.forename: M
authors.initials: M
authors.lastname: Snyder
mesh_terms: D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002453:Cell Cycle; D016213:Cyclins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D016193:G1 Phase; D015966:Gene Expression Regulation, Fungal; D020928:Mitogen-Activated Protein Kinases; D010766:Phosphorylation; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D016196:S Phase; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic; D015003:Yeasts / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D016213:Cyclins; D005656:Fungal Proteins; C090559:PCL2 protein, S cerevisiae; C081540:SBF protein, S cerevisiae; C075111:SWI6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C070839:PCL1 protein, S cerevisiae; C097993:Pkc1 protein, Trichoderma reesei; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; C072724:SLT2 protein, S cerevisiae