Aggravation of murine experimental allergic encephalomyelitis by administration of T-cell receptor gammadelta-specific antibody.
Experimental allergic encephalomyelitis (EAE) is thought to be dominantly mediated by Ag-specific CD4+ MHC class II-restricted T-cells. Recent reports demonstrated accumulation of gammadelta T-cells in active multiple sclerosis (MS) plaque and infiltration into brains with EAE. However, the role of gammadelta T-cells in pathogenesis of EAE remains unknown. In the present study we have examined EAE mice administered T-cell receptor (TCR) gammadelta-specific mAb (UC7-13D5) to elucidate the potential role of gammadelta T-cells in the pathogenesis of EAE. MAb treatment led to transient depleting gammadelta T-cells in vivo. MAb-treated EAE mice showed aggravation and disease recurrence and also increased Ag-specific proliferative responses. Semiquantitative PCR analysis demonstrated an increased level of IFN-gamma mRNA expression in splenocytes from mAb-treated EAE mice during the induction and pre-relapse phase, however, aggravation and disease recurrence have not been suggested to be directly mediated by IFN-gamma in the present study. Our results imply that gammadelta T-cells play a preventing role in the recurrence of EAE.
Kobayashi
Y
Y
Department of Neurology, Nagoya University School of Medicine, Showa-ku, Japan. yakoba@tsuru.med.nagoya-u.ac.jp
Kawai
K
K
Ito
K
K
Honda
H
H
Sobue
G
G
Yoshikai
Y
Y
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology / Q000503:physiopathology*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000473:pathology; D014158:Transcription, Genetic
D000906:Antibodies; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D007371:Interferon-gamma