The effect of sterols on the sensitivity of membranes to the channel-forming antifungal antibiotic, syringomycin E.
The ability of three sterols of different structure to influence the interaction of syringomycin E (an antifungal antibiotic that forms voltage dependent channels in planar lipid bilayers) with a planar lipid bilayer was evaluated. The rate of increase of bilayer conductance induced by syringomycin E was about 1000-times less in bilayers containing 50 mol% of cholesterol compared to bilayers without sterols. The effect of ergosterol (the primary sterol of fungal cells) on the sensitivity of bilayers to syringomycin E was much weaker than that of cholesterol, while stigmasterol (one of the main sterols of plant cells) did not significantly influence the ability of syringomycin E to induce a conductance increase in the bilayer. None of the sterols altered the single channel conductance properties of syringomycin E. These observations suggest that cholesterol affects the sensitivity of target membranes to syringomycin E by enlarging the energy barrier for channel formation rather than participating in channel formation itself.
Feigin
A M
AM
Monell Chemical Senses Center, Philadelphia, PA 19104-3308, USA. feigin@pobox.upenn.edu
Schagina
L V
LV
Takemoto
J Y
JY
Teeter
J H
JH
Brand
J G
JG
D000935:Antifungal Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D002782:Cholestenes / Q000494:pharmacology*; D004553:Electric Conductivity; D007473:Ion Channels / Q000187:drug effects* / Q000502:physiology; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines
D000935:Antifungal Agents; D001426:Bacterial Proteins; D002782:Cholestenes; D007473:Ion Channels; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; C032677:syringomycin; C042511:1,2-dioleoylphosphatidylserine; C020888:1,2-dielaidoylphosphatidylethanolamine