title: The C-terminus of glycosylphosphatidylinositol-specific phospholipase D is essential for biological activity.
abstract: Glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) (EC 3.1.4.50) from mammalian serum is a 115 kDa glycoprotein consisting of 816 amino acids. We found that C-terminal deletions of only two to five amino acids reduced GPI-PLD enzymatic activity by roughly 70% as compared to wild-type protein. C-terminal deletions of more than five amino acids resulted in a complete loss of GPI-PLD enzymatic activity. Point mutations at position 811 indicate that Tyr-811 may play a major role in maintaining the biological activity of GPI-PLD.
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Bern, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Stadelmann
authors.forename: P
authors.initials: P
authors.lastname: Bütikofer
authors.forename: A
authors.initials: A
authors.lastname: König
authors.forename: U
authors.initials: U
authors.lastname: Brodbeck
mesh_terms: D000818:Animals; D019556:COS Cells; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics; D010739:Phospholipase D / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014357:Trypsin; D014443:Tyrosine / Q000378:metabolism
chemical_list: D017261:Glycosylphosphatidylinositols; D010446:Peptide Fragments; D014443:Tyrosine; D010739:Phospholipase D; C065017:glycoprotein phospholipase D; D014357:Trypsin