[Expression of synthetic gene for human angiogenin in E. coli cells].
A synthetic gene for human angiogenin was cloned into pRTang under control of the Proteus mirabilis recA promoter. After induction with mitomycin C, Escherichia coli cells transformed with pRTang produced an additional 14.2-kDa protein. According to electrophoretic and immunochemical analyses, this protein was identical to human angiogenin.
Nikonova
A A
AA
Seregin
S V
SV
Chikaev
N A
NA
Mishin
V P
VP
Babkina
I N
IN
Mertvetsov
N P
NP
D043925:Angiogenesis Inducing Agents / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005813:Genes, Synthetic; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D011513:Proteus mirabilis / Q000235:genetics; D012259:Ribonuclease, Pancreatic
D043925:Angiogenesis Inducing Agents; D011506:Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic
Ekspressiia sinteticheskogo gena angiogenina cheloveka v kletkakh E. coli.