title: [The use of pluripotent mouse embryo stem cells for the production of chimeric animals].
abstract: Embryonic stem (ES) cells derived from pluripotent cells of the early mouse embryos provide a powerful tool for genome manipulation in mammals. Conditions for maintaining and preservation of pluripotent properties of embryonic stem (ES) cells in culture using different tests are described. A simple aggregation of pluripotent ES cells with morulae-stage embryos for derivation of chimaeras is considered.
authors.forename: I P
authors.initials: IP
authors.lastname: Savchenkova
authors.forename: Z M
authors.initials: ZM
authors.lastname: Fleischmann
authors.forename: J
authors.initials: J
authors.lastname: Bulla
authors.forename: G
authors.initials: G
authors.lastname: Brem
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002449:Cell Aggregation; D002478:Cells, Cultured; D002678:Chimera; D003584:Cytological Techniques; D005260:Female; D016256:Lewis X Antigen / Q000032:analysis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009028:Morula / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology / Q000276:immunology
chemical_list: D016256:Lewis X Antigen; D000469:Alkaline Phosphatase
vernacular_title: Ispol'zovanie pliuripotentnykh émbrional'nykh stvolovykh kletok myshi dlia polucheniia khimernykh zhivotnykh.