Use of antibodies against the human antigen of erythroblasts for the detection of nucleated erythrocytes in the maternal circulation.
In the present study, we tried to detect nucleated erythrocytes in maternal blood by means of the HAE9 antibody that binds to the antigen of erythroblasts (Ag-Eb) on nucleated erythroid cells. Umbilical cord blood and placental cells, which are known to contain a high number of nucleated erythrocytes, indeed demonstrated a high percentage of Ag-Eb-positive cells. In comparison, HAE9 stained up to 4.5% of the peripheral blood cells collected from pregnant women, but not those of nonpregnant women or normal men. These results, demonstrating a simple and reliable method for the identification of nucleated erythrocytes in the maternal circulation using the anti-Ag-Eb antibody, might open new possibilities for the detection of nucleated erythrocytes in maternal blood.
Savion
S
S
Department of Embryology and Teratology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel.
Carp
H
H
Shepshelovich
J
J
Irlin
J
J
Kostykov
M
M
Fein
A
A
Toder
V
V
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000097:blood; D002467:Cell Nucleus; D004900:Erythroblasts / Q000276:immunology*; D004912:Erythrocytes / Q000648:ultrastructure*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D000941:Antigens