Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization.
The peroxidase-mediated deposition of hapten- and fluorochrome-labeled tyramides has recently been shown to increase the sensitivity of immunofluorescence and fluorescence in situ hybridization techniques. We have evaluated a number of red, green, and blue fluorescent tyramides for detection of antigens in tissue sections and cytospin preparations and for the detection of hapten- and horseradish peroxidase-labeled probes hybridized in situ to cells and chromosomes. With few exceptions, all fluorescent tyramide-based methods provided a considerable increase in sensitivity compared to conventional immunofluorescence and FISH methods.
van Gijlswijk
R P
RP
Department of Cytochemistry and Cytometry, Sylvius Laboratory, Leiden University, The Netherlands.
Zijlmans
H J
HJ
Wiegant
J
J
Bobrow
M N
MN
Erickson
T J
TJ
Adler
K E
KE
Tanke
H J
HJ
Raap
A K
AK
D000818:Animals; D000941:Antigens / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051381:Rats; D012680:Sensitivity and Specificity; D014439:Tyramine / Q000737:chemistry*; D014746:Vimentin / Q000032:analysis
D000941:Antigens; D005456:Fluorescent Dyes; D014746:Vimentin; D006735:Horseradish Peroxidase; D014439:Tyramine