Identification of a Zn(2+)-sensitive component of Ehrlich cell plasma membrane redox system by CHAPS-agarose-polyacrylamide electrophoresis and in situ staining of activity.
A procedure based on CHAPS-agarose-polyacrylamide electrophoresis and in situ staining of activity was used to detect a Zn(2+)-sensitive component of Ehrlich cell plasma membrane redox system. The procedure is so powerful that it allows to use crude plasma membrane fractions and can be easily adapted for use in an electrophoretic approach to the purification of this protein.
Rodríguez-Caso
L
L
Laboratorio de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
Rodríguez-Agudo
D
D
del Castillo-Olivares
A
A
Márquez
J
J
Núñez de Castro
I
I
Medina
M A
MA
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002462:Cell Membrane / Q000201:enzymology*; D002793:Cholic Acids; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D051379:Mice; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*; D014407:Tumor Cells, Cultured; D015032:Zinc
D002793:Cholic Acids; D010088:Oxidoreductases; D015032:Zinc; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate