[Expression of various monoclonal antibodies in nodules and band stage in Dupuytren's disease].
In 42 seronegative patients (n = 6 normal fascia, n = 12 nodal stage of Dupuytren's contracture, n = 24 stage IV) we performed immunohistochemical examinations of specimens of four locations of the digitopalmar fascia by means of indirect immunofluorescence tests. We determined the expression of monoclonal antibodies against vimentin, leucocyte common antigen (LCA, CD 45), four macrophage-antigens (27E10, RM3/1, 25F9, CD68), PDGFR-beta subunit (platelet-derived growth factor receptor). In 12 specimens of nodal stage disease macrophage-antigens typical for late inflammatory phase was detected. Other macrophage phenotypes were found to a lesser degree. All of these antigens were negative in normal palmar fascia and only sporadically positive in stage IV disease. PDGFR, which has not been investigated in digitopalmar flexion contracture before, has been expressed in cells of Dupuytren's disease as well as in normal fascia to a minor degree. In nodal stage disease, PDGFR-expression was not generally increased, although the majority of these cases showed intensively dyed clusters. EGFR could neither be detected in nodal or cord-stage of Dupuytren's disease, nor in normal digitopalmar fascia.
Brenner
P
P
Klinik für Plastische, Hand- und Wiederherstellungschirurgie der Medizinischen Hochschule Hannover.
Sachse
C
C
Reichert
B
B
Berger
A
A
D000911:Antibodies, Monoclonal; D004387:Dupuytren Contracture / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017493:Leukocyte Common Antigens / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000276:immunology; D011446:Prospective Studies; D017479:Receptors, Platelet-Derived Growth Factor / Q000276:immunology; D014746:Vimentin / Q000276:immunology
D000911:Antibodies, Monoclonal; D010982:Platelet-Derived Growth Factor; D014746:Vimentin; D017479:Receptors, Platelet-Derived Growth Factor; D017493:Leukocyte Common Antigens
Expression von diversen monoklonalen Antikörpern im Knoten- und Strangstadium des Morbus Dupuytren.