title: Protocol for site-directed mutagenesis in the preparation of large gene-targeting vectors.
authors.affiliation: University of Arkansas for Medical Sciences, Little Rock, USA. ppdetogni@acrc.uams.edu
authors.forename: P
authors.initials: P
authors.lastname: De Togni
mesh_terms: D000818:Animals; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors; D051379:Mice; D008822:Mice, Transgenic; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D018167:Receptors, Calcitriol / Q000235:genetics; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D017931:DNA Primers; D018167:Receptors, Calcitriol; D014764:Viral Proteins; C069983:gene 43 protein, Enterobacteria phage T4; D004259:DNA-Directed DNA Polymerase; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases