Differential regulation of fibroblast growth factor (FGF)-2 and FGF receptor 1 mRNAs and FGF-2 isoforms in spinal ganglia and sciatic nerve after peripheral nerve lesion.
To study the functional role of endogenous basic fibroblast growth factor-2 (FGF-2) during degeneration and regeneration of the sensory system, we have determined the expression and regulation of FGF-2 and FGF receptor (FGFR)-1 mRNAs in spinal ganglia and sciatic nerve during experimental transection and crush injury of the sciatic nerve. In contrast to levels of the FGFR-1 transcript, which is not altered, the level of FGF-2 mRNA is dramatically up-regulated in spinal ganglia after injury. In the proximal and distal nerve stumps both transcript levels are significantly elevated, albeit at different time points. The FGF-2 isoforms are differently up-regulated in spinal ganglia and sciatic nerve following peripheral nerve lesion. The differential response of FGF-2 mRNA and protein and of FGFR-1 mRNA in spinal ganglia and sciatic nerve after lesion is suggestive of different physiological functions: a local reaction at the lesion site where axonal regrowth occurs and a trophic reaction for the degenerating/regenerating sensory neurons.
Meisinger
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Institute of Anatomy, University of Freiburg, Germany.
Grothe
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D000818:Animals; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D005727:Ganglia, Spinal / Q000378:metabolism*; D007536:Isomerism; D009409:Nerve Crush; D009416:Nerve Regeneration; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics*; D012584:Sciatic Nerve / Q000378:metabolism*
D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2