Biology of Amazonian anopheline. XX. Ontogeny of esterases, leucine aminopeptidase and alpha-glycerophosphate dehydrogenase in Anopheles (Nyssorhynchus) darlingi Root, 1926 (Diptera, Culicidae).
The esterases, leucine aminopeptidase and alpha-glycerophosphate dehydrogenase revealed modifications in gene expressions during the development of Anopheles darlingi. The esterases showed five activity bands, 1 and 2 being more deeply stained during the larval stages than in pupae or adults, esterases 3 and 4 more deeply stained in pupae and adults whereas esterase 5 was present throughout development. Leucine aminopeptidase showed five activity bands: LAP2 and LAP5 were characteristic of larvae, LAP3 was specific for pupae and adults, LAP4 was detected only in pupae, and LAP1 and LAP6 were detected in all stages. alpha-Glycerophosphate dehydrogenase presented one activity band on starch gel whose intensity increased with development. Two activity bands were detected on polyacrylamide gel (alpha-GPDH1 and alpha-GPDH2) in 4th-instar larvae (old pigmented larvae) and this activity increased with development.
Dos Santos
J M
JM
INPA, C.P.478, Manaus, AM, Brasil.
Tadei
W P
WP
Contel
E P
EP
D000818:Animals; D000852:Anopheles / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D004950:Esterases / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D007931:Leucyl Aminopeptidase / Q000235:genetics* / Q000378:metabolism
D005993:Glycerolphosphate Dehydrogenase; D004950:Esterases; D007931:Leucyl Aminopeptidase