Repression of the 5-lipoxygenase gene by c-myb overexpression in differentiated HL-60 cells.
This paper reports on the involvement of c-MYB in the regulation of 5-lipoxygenase gene expression during differentiation of human HL-60 cells. We demonstrate that c-MYB binds the 5-lipoxygenase promoter in undifferentiated cells but not in DMSO-differentiated cells. Also, we show that overexpression of c-myb cDNA in differentiated HL-60 cells represses the 5-lipoxygenase gene expression.
Ponton
A
A
Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, P.Q., Canada.
Thirion
J P
JP
Sirois
P
P
D001094:Arachidonate 5-Lipoxygenase / Q000187:drug effects / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D011994:Recombinant Proteins; D015534:Trans-Activators; D001094:Arachidonate 5-Lipoxygenase; D004121:Dimethyl Sulfoxide