Induction of cyclooxygenase-2 mRNA and protein following transient focal ischemia in the rat brain.
Expression of cyclooxygenase-2 (cox-2) mRNA and inducible heat-shock protein-70 (hsp-70) mRNA was studied with in situ hybridization techniques at 30 min and 4 h following 1 h transient middle cerebral artery (MCA) occlusion in the rat brain. In addition, immunoreactivity for cox-2 was studied after 8 h of reperfusion. Induction of hsp-70 and cox-2 mRNA was found in the brain side ipsilateral to MCA occlusion. Hsp-70 mRNA was induced in the parietal cortex and striatum within the territory of the occluded MCA. Induction of cox-2 mRNA was particularly seen in cortical layer II in the brain side ipsilateral to MCA occlusion. At 30 min of reperfusion, areas showing cox-2 mRNA induction included the cingulate and frontal cortices located perifocally to the areas showing hsp-70 mRNA induction, and the piriform cortex. At 4 h of reperfusion, induction of cox-2 mRNA was seen within the parietal cortex. At 8 h of reperfusion, immunoreactivity for cox-2 was mainly seen in the ipsilateral cortex. These results demonstrate that transient focal ischemia induces the expression of cox-2 mRNA and protein in discrete areas of the rat brain during reperfusion, which might lead to local increases of arachidonic acid metabolism.
Planas
A M
AM
Departament de Farmacologia i Toxicologia, CID, Barcelona, Spain.
Soriano
M A
MA
Rodríguez-Farré
E
E
Ferrer
I
I
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D004790:Enzyme Induction / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D011451:Prostaglandin-Endoperoxide Synthases