title: Crystallization and preliminary X-ray diffraction studies of aSFP, a bovine seminal plasma protein with a single CUB domain architecture.
abstract: Bovine acidic seminal fluid protein (aSFP) is a 1.29 kDa polypeptide of the spermadhesin family built by a single CUB domain architecture. The CUB domain is an extracellular module present in 16 functionally diverse proteins. To determine the three-dimensional structure of aSFP, the protein was crystallized at 21 degrees C by vapor diffusion in hanging drops, using ammonium sulfate, pH 4.7, and polyethyleneglycol 4,000 as precipitants, containing 10% dioxane to avoid the formation of clustered crystals. Elongated prismatic crystals with maximal size of 0.6 x 0.3 x 0.2 mm3 diffract to beyond 1.9 A resolution and belong to space group P2(1)2(1)2(1), with cell parameters a = 52.4 A, b = 41.5 A, c = 48.2 A. There is one aSFP molecule per asymmetric unit, which corresponds to a crystal volume per unit molecular mass of 2.04 A3/Da, and analytical ultracentrifugation analysis show that aSFP is a monomeric protein.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Oeiras, Portugal.
authors.forename: J M
authors.initials: JM
authors.lastname: Dias
authors.forename: A L
authors.initials: AL
authors.lastname: Carvalho
authors.forename: I
authors.initials: I
authors.lastname: Kölln
authors.forename: J J
authors.initials: JJ
authors.lastname: Calvete
authors.forename: E
authors.initials: E
authors.lastname: Töpfer-Petersen
authors.forename: P F
authors.initials: PF
authors.lastname: Varela
authors.forename: A
authors.initials: A
authors.lastname: Romero
authors.forename: C
authors.initials: C
authors.lastname: Urbanke
authors.forename: M J
authors.initials: MJ
authors.lastname: Romão
mesh_terms: D000818:Animals; D002417:Cattle; D003460:Crystallization; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000737:chemistry*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D013816:Thermodynamics; D014961:X-Ray Diffraction
chemical_list: D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein