Establishment and characterization of an abrin-resistant cell line.
An abrin-resistant cell line, CHOR 3-4, was isolated from CHOK1 cells which were resistant to a high concentration of abrin (160 ng/ml), and had a 1,000-fold higher resistance to abrin that of CHOK1 cells. CHOR 3-4 cells were about 25-fold more resistant than CHOK1 cells to the N-glycosidase activity of abrin, which was measured by hydrolyzing the N-glycosidic bond of adenine 4324 nucleotide from 3' end of mammalian 28S rRNA. However, the isolated polysomes of CHOR 3-4 cells had the same sensitivity to abrin as those of CHOK1 cells. On measuring the binding of 125I-abrin to CHOR 3-4 cells, it was decreased to about 20% that of CHOK1 cells. This indicates that the mechanism of the resistance of CHOR 3-4 to abrin is due to the alteration of glycoproteins or glycolipids of cell membrane.
Ko
J L
JL
Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, ROC.
Lin
J Y
JY
D000036:Abrin / Q000378:metabolism / Q000633:toxicity*; D002460:Cell Line; D004351:Drug Resistance; D011132:Polyribosomes / Q000187:drug effects
D000036:Abrin