Monoclonal yeast killer toxin-like candidacidal anti-idiotypic antibodies.
Rat monoclonal yeast killer toxin (KT)-like immunoglobulin M (IgM) anti-idiotypic antibodies (KT-IdAbs) were produced by idiotypic vaccination with a mouse monoclonal antibody (MAb; MAb KT4) that neutralized a Pichia anomala KT characterized by a wide spectrum of antimicrobial activity. The characteristics of the KT-IdAbs were demonstrated by their capacity to compete with the KT to the idiotype of MAb KT4 and to interact with putative KT cell wall receptors (KTRs) of sensitive Candida albicans cells. The internal-image properties of KT-IdAbs were proven by their killer activity against KT-sensitive yeasts. This lethal effect was abolished by prior adsorption of KT-IdAbs with MAb KT4. These findings stressed the potential importance of antibody-mediated immunoprotection against candidiasis and suggested a feasible experimental approach for producing antimicrobial receptor antibodies without purifying the receptor. KT-IdAbs might represent the basis for producing engineered derivatives with a high potential for effective therapeutic antifungal activity.
Polonelli
L
L
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università degli Studi di Parma, Italy. lucpol@ipruniv.cce.unipr.it
Séguy
N
N
Conti
S
S
Gerloni
M
M
Bertolotti
D
D
Cantelli
C
C
Magliani
W
W
Cailliez
J C
JC
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology / Q000628:therapy; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007075:Immunoglobulin M; D007167:Immunotherapy; D055646:Killer Factors, Yeast; D051379:Mice; D009183:Mycotoxins / Q000276:immunology*; D010843:Pichia / Q000276:immunology; D051381:Rats
D000888:Antibodies, Anti-Idiotypic; D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D055646:Killer Factors, Yeast; D009183:Mycotoxins