title: Mitoxantrone-induced DNA strand breaks in cell-cultures of malignant human astrocytoma and glioblastoma tumors.
abstract: In this study mitoxantrone (Mtx) induced DNA strand breaks were measured with the alkaline elution technique in short term cell cultures derived from human gliomas. Glioblastomas or astrocytomas from 5 patients who underwent intracranial surgery were cultured and incubated i h with different concentrations of Mtx (0, 0.01, 0.1 and 1.0 microgram/ml). The alkaline elution method was modified to measure DNA lesions in human gliomas. Mtx induced DNA strand breaks in a dose dependent manner in all cell cultures tested. There was a linear increase of DNA strand break frequency induced by Mtx between 0.01-1.0 microgram/ml. concerning these in vitro data, Mtx might be potentially useful for the treatment of patients with malignant brain tumors.
authors.affiliation: Neurosurgical Department, University of Giessen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Senkal
authors.forename: J C
authors.initials: JC
authors.lastname: Tonn
authors.forename: R
authors.initials: R
authors.lastname: Schönmayr
authors.forename: W
authors.initials: W
authors.lastname: Schachenmayr
authors.forename: U
authors.initials: U
authors.lastname: Eickhoff
authors.forename: M
authors.initials: M
authors.lastname: Kemen
authors.forename: E
authors.initials: E
authors.lastname: Kollig
mesh_terms: D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000235:genetics*; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects*; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D008942:Mitoxantrone / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D008942:Mitoxantrone