title: In vitro activation of mouse spleen cells by a lysate of Theileria sergenti-infected bovine red blood cells.
abstract: A lysate of Theileria sergenti-infected bovine red blood cells induced in vitro proliferation of mouse spleen cells in a 2-3-day incubation period. Experiments using B or T cell-enriched preparations showed that a B cell (B220+ CD3- population) was responsible for this non-specific proliferation. In the culture supernatant of spleen cells stimulated with the lysate, considerable amounts of interleukin 2 (IL-2) and gamma-interferon (IFN-gamma) were revealed after 2 days, followed by production of immunoglobulin with a peak at 4-6 days. Thus, it was suggested that a lysate antigen(s) of T. sergenti-infected red blood cells was able to stimulate mouse spleen cells to produce IL-2 and IFN-gamma, which in turn induced the proliferation of B cells and production of immunoglobulin.
authors.affiliation: Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yanagisawa
authors.forename: T
authors.initials: T
authors.lastname: Kamiyama
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000469:parasitology*; D006461:Hemolysis; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology*; D013156:Splenectomy; D013601:T-Lymphocytes / Q000276:immunology*; D016794:Theileria / Q000276:immunology*
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma