Nitric oxide and beta-adrenergic agonist-induced bronchial arterial vasodilation.
In anesthetized sheep, we measured bronchial blood flow (Qbr) by an ultrasonic flow probe to investigate the interaction between inhaled nitric oxide (NO; 100 parts/million) given for 5 min and 5 ml of aerosolized isoetharine (1.49 x 10(-2) M concentration). NO and isoetharine increased Qbr from 26.5 +/- 6.5 to 39.1 (SE) +/- 10.6 and 39.7 +/- 10.7 ml/min, respectively (n = 5). Administration of NO immediately after isoetharine further increased Qbr to 57.3 +/- 15.1 ml/min. NO synthase inhibitor N(omega)-nitro-L-arginine methyl ester hydrochloride (L-NAME; 30 mg/kg, in 20 ml saline given i.v.) decreased Qbr to 14.6 +/- 2.6 ml/min. NO given three times alternately with isoetharine progressively increased Qbr from 14.6 +/- 2.6 to 74.3 +/- 17.0 ml/min, suggesting that NO and isoetharine potentiate vasodilator effects of each other. In three other sheep, after L-NAME three sequential doses of isoetharine increased Qbr from 10.2 +/- 3.4 to 11.5 +/- 5.7, 11.7 +/- 4.7, and 13.3 +/- 5.7 ml/min, respectively, indicating that effects of isoetharine are predominantly mediated through synthesis of NO. When this was followed by three sequential administrations of NO, Qbr increased by 146, 172, and 185%, respectively. Thus in the bronchial circulation, there seems to be a close interaction between adenosine 3',5'-cyclic monophosphate- and guanosine 3',5'-cyclic monophosphate-mediated vasodilation.
Charan
N B
NB
Pulmonary Research Laboratory, Veterans Affairs Medical Center, Boise, Idaho 83702, USA. ncharan@micron.net
Johnson
S R
SR
Lakshminarayan
S
S
Thompson
W H
WH
Carvalho
P
P
D000818:Animals; D001981:Bronchial Arteries / Q000187:drug effects*; D007528:Isoetharine / Q000494:pharmacology*; D009569:Nitric Oxide / Q000494:pharmacology*; D012756:Sheep; D014664:Vasodilation / Q000187:drug effects*
D009569:Nitric Oxide; D007528:Isoetharine