Cloning of the human Gfi-1 gene and its mapping to chromosome region 1p22.
Recently the rat and mouse Growth Factor Independence (Gfi-1) genes have been cloned (Gilks et al., 1993; Zoring et al; 1996). This gene allows cells in culture to overcome the depletion of growth factors in the culture medium and maintain their proliferative potential. As part of a cloning strategy to isolated genes from human chromosome 1p22 which are associated with a constitutional chromosome translocation from a patient with stage 4S neuroblastoma, we have identified the human homologue of the Gfi gene and defined a 50 Kb map position within a well characterised YAC contig from the region. The full length cDNA sequence is 81% homologous with the rodent counterparts and, at the protein level, is even more highly conserved.
Roberts
T
T
Department of Neurosciences, Research Institute, Cleveland Clinic Foundation, Ohio 44195, USA.
Cowell
J K
JK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
D004268:DNA-Binding Proteins; C498275:GFI1 protein, human; C498276:Gfi1 protein, rat; D014157:Transcription Factors