Alterations in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV in quinolone-resistant clinical isolates of Klebsiella pneumoniae.
We determined a partial sequence of the Klebsiella pneumoniae parC gene, including the region analogous to the quinolone resistance-determining region of the Escherichia coli gyrA gene, and examined 26 clinical strains of K. pneumoniae for an association of alterations in GyrA and ParC with susceptibilities to quinolones. The study suggests that in K. pneumoniae DNA gyrase is a primary target of quinolones and that ParC alterations play a complementary role in the development of higher-level fluoroquinolone resistance.
Deguchi
T
T
Department of Urology, Gifu University School of Medicine, Japan.
Fukuoka
A
A
Yasuda
M
M
Nakano
M
M
Ozeki
S
S
Kanematsu
E
E
Nishino
Y
Y
Ishihara
S
S
Ban
Y
Y
Kawada
Y
Y
D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology; D001483:Base Sequence; D002511:Cephalosporins / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D027081:DNA Gyrase; D027101:DNA Topoisomerase IV; D004250:DNA Topoisomerases, Type II / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D016133:Polymerase Chain Reaction
D000890:Anti-Infective Agents; D002511:Cephalosporins; D009268:Nalidixic Acid; D002939:Ciprofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II