Human interleukin-2 could bind to opioid receptor and induce corresponding signal transduction.
Interleukin-2 (IL-2) was found to have an analgesic effect in the peripheral nervous system. Both electrophysiological and behavioural experiments suggested an anti-noceiceptive effect of IL-2 in animals. Biochemical experiments revealed that IL-2 could displace diprenorphine binding in both NG 108-15 cells and HEK 293 cells transfected with pCDNA3-DOR (delta opioid receptor). Furthermore, IL-2 significantly inhibited cAMP production stimulated by forskolin in both NG 108-15 and transfected HEK 293 cells, indicating that the binding of IL-2 to the delta opioid receptor is functional.
Wang
Y
Y
Shanghai Institutes of Biochemistry, China.
Pei
G
G
Cai
Y C
YC
Zhao
Z Q
ZQ
Wang
J B
JB
Jiang
C L
CL
Zheng
Z C
ZC
Liu
X Y
XY
D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002415:Cats; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D004174:Diprenorphine / Q000493:pharmacokinetics; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism* / Q000494:pharmacology*; D008297:Male; D009292:Narcotic Antagonists / Q000493:pharmacokinetics; D009434:Neural Pathways / Q000187:drug effects; D017933:Peripheral Nervous System / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D011957:Receptors, Opioid / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D017465:Receptors, Opioid, delta / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D014162:Transfection
D000700:Analgesics; D007376:Interleukin-2; D009292:Narcotic Antagonists; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D004174:Diprenorphine; D000242:Cyclic AMP