title: Effects of recombinant bactericidal, permeability-increasing protein on bacterial translocation and pulmonary neutrophil sequestration in burned mice.
abstract: Burn injury induces bacterial translocation (BT) from the gut in multiple animal models. Etiologic factors contributing to BT may be an ischemia-reperfusion injury to the gut, the release of inflammatory cytokines, oxygen metabolites and other mediators, and cytotoxic effects mediated by endotoxin (lipopolysaccharide). Bactericidal, permeability-increasing protein is a neutrophil granule protein with potent bactericidal and lipopolysaccharide-neutralizing activities. The use of this protein has not been previously reported in a burn-injury model. The purpose of this study was to determine whether recombinant bactericidal, permeability-increasing protein (rBPI23) affects the incidence of BT and myeloperoxidase content in lung tissue (a measure of leukocyte sequestration) in a burn-injury model. Mice received a 32% total body surface area, full-thickness, scald burn, and 10 mg/kg body weight rBPI23 in saline solution was given by intraperitoneal injection at 0, 3, and 6 hours after the burn. Control animals received intraperitoneal saline solution only. All animals received a total of 1 ml saline solution intraperitoneally immediately after burn injury for fluid resuscitation. At 24 hours after burn injury, mesenteric lymph nodes (MLN) were harvested, homogenized, and plated. Lung tissue was harvested and assayed for myeloperoxidase. Burned mice treated with rBPI23 had significantly (p = 0.005, Fisher's Exact Test, two-tailed) decreased incidence of BT, compared to burned mouse controls. Leukosequestration into lung tissues was not affected by rBPI23. Postburn administration of rBPI23 reduces but does not abolish the incidence of BT after burn injury in mice, perhaps by reducing intestinal injury during burn shock and the ischemia-reperfusion period by inhibiting the effects of lipopolysaccharide. An alternate explanation may be that rBPI23 could increase clearance and killing of bacteria by host defenses.
authors.affiliation: Department of Surgery, University of California, San Diego Medical Center 92103, USA.
authors.forename: O H
authors.initials: OH
authors.lastname: Rennekampff
authors.forename: M
authors.initials: M
authors.lastname: Tenenhaus
authors.forename: J
authors.initials: J
authors.lastname: Hansbrough
authors.forename: V
authors.initials: V
authors.lastname: Kiessig
authors.forename: R L
authors.initials: RL
authors.lastname: Zapata-Sirvent
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D018988:Bacterial Translocation; D001798:Blood Proteins / Q000494:pharmacology*; D002056:Burns / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology*; D005260:Female; D008168:Lung / Q000201:enzymology / Q000473:pathology*; D008198:Lymph Nodes / Q000382:microbiology; D008565:Membrane Proteins; D051379:Mice; D009504:Neutrophils / Q000473:pathology*; D009195:Peroxidase / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology*; D012774:Shock, Traumatic / Q000209:etiology / Q000382:microbiology / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C021623:bactericidal permeability increasing protein; D009195:Peroxidase