Horseradish peroxidase-labeled oligonucleotides and fluorescent tyramides for rapid detection of chromosome-specific repeat sequences.
We present a sensitive and rapid fluorescence in situ hybridization (FISH) strategy for detecting chromosome-specific repeat sequences. It uses horseradish peroxidase (HRP)-labeled oligonucleotide sequences in combination with fluorescent tyramide-based detection. After in situ hybridization, the HRP conjugated to the oligonucleotide probe is used to deposit fluorescently labeled tyramide molecules at the site of hybridization. The method features full chemical synthesis of probes, strong FISH signals, and short processing periods, as well as multicolor capabilities.
van Gijlswijk
R P
RP
Department of Cytochemistry and Cytometry, Leiden University, The Netherlands.
Wiegant
J
J
Vervenne
R
R
Lasan
R
R
Tanke
H J
HJ
Raap
A K
AK
D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human / Q000235:genetics / Q000648:ultrastructure*; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D015345:Oligonucleotide Probes; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D014443:Tyrosine / Q000031:analogs & derivatives*
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; C105109:TSA-Direct BlueFISH; C105107:TSA-Direct GreenFISH; C105108:TSA-Direct RedFISH; D014443:Tyrosine; D006735:Horseradish Peroxidase