Upregulation of actin gene expression in cells expressing exogenous beta-amyloid precursor protein.
We investigated the effects of beta-amyloid peptide precursor (APP) overexpression upon the levels of other mRNAs. Using quantitative slot-blot hybridization and immunoblot analysis we observed that enhanced levels of APP elevated the levels of beta-actin and beta-actin mRNA. Our results also suggest that the cytoplasmic domain of APP is crucial for the elevation in beta-actin gene expression.
Ramakrishna
N
N
NYS Institute for Basic Research in Developmental Disabilities, Staten Island 10314, USA.
Smedman
M
M
Ramakrishna
V
V
Gillam
B
B
D000199:Actins / Q000235:genetics*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D015870:Gene Expression; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D000199:Actins; D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger; D011994:Recombinant Proteins