Highly sensitive method to detect mRNAs in individual cells by direct RT-PCR using Tth DNA polymerase.
A new method for detecting the expression of low-abundance mRNA molecules has been developed that combines the sensitivity of PCR, the high efficiency and specificity of reverse transcription (RT) using Tth DNA polymerase at high temperature, and the enhancement of sensitivity and specificity of nested PCR. This method is highly sensitive, reproducible and allows the detection of mRNAs in individual cells by direct RT-PCR.
Chiocchia
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INSERM Unité 283, Hôpital COCHIN Paris, France.
Smith
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KA
D000818:Animals; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006358:Hot Temperature; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D013945:Thymoma; D013953:Thymus Neoplasms; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase