title: Subunit change in cytochrome c oxidase: identification of the oxygen switch in Dictyostelium.
abstract: Cytochrome c oxidase (COX) has a complex modular structure in eukaryotes. Depending on growth conditions, interchangeable isoforms of selected subunits are synthesized and combined to the evolutionarily conserved catalytic core of the enzyme. In Dictyostelium this structural make-up is regulated by oxygen and involves two forms of the smallest subunit, termed VIIe and VIIs. Here we show that, in spite of a considerable sequence divergency, they are encoded by adjacent genes, linked 'tail to head' by only 800 bp. Deletion analyses reveal the presence of a short intergenic segment acting as an oxygen transcriptional switch. This structural organization and the different stability of the two subunit isoforms offer a molecular explanation for the extraordinary sensitivity to oxygen of the switching mechanism.
authors.affiliation: Centro CNR Biomembrane and Dipartimento di Scienze Biomediche Sperimentali, Padova, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Bisson
authors.forename: S
authors.initials: S
authors.lastname: Vettore
authors.forename: E
authors.initials: E
authors.lastname: Aratri
authors.forename: D
authors.initials: D
authors.lastname: Sandona
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000235:genetics; D004023:Dictyostelium / Q000201:enzymology* / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics; D008969:Molecular Sequence Data; D010100:Oxygen / Q000494:pharmacology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D016053:RNA, Protozoan / Q000032:analysis; D015183:Restriction Mapping; D017384:Sequence Deletion; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D016054:DNA, Protozoan; D012333:RNA, Messenger; D016053:RNA, Protozoan; D003576:Electron Transport Complex IV; D010100:Oxygen