The highly immunogenic enolase and Hsp70p are adventitious Candida albicans cell wall proteins.
Screening cDNA libraries with polyclonal antibody preparations against Candida albicans yeast or mycelial cell walls resulted in isolation of several positive clones. Some of them encoded enolase; others encoded a protein of the 70 kDa heat-shock protein family (Hsp70p), etc. The presence of these cytosolic proteins in the cell wall of actively growing C. albicans was discovered by analytical (SDS-PAGE and Western blot) and cytological (indirect immunofluorescence) experiments. Supplementation of cell cultures with papulacandin B, an antibiotic that inhibits formation of the beta-glucan skeleton, resulted in the release of enolase to the supernatant fluids; this release was prevented when 0.6 M KCl was present as an osmotic stabilizer. The cell wall of C. albicans incorporated exogenously added proteins (enolase and Escherichia coli and C., albicans cytosolic proteins). The presence in the C. albicans cell wall of enolase, Hsp70p, and probably other intracellular proteins that are highly immunogenic might help the fungal cells to evade the host defences, and consequently could represent a survival mechanism for C. albicans 'in vivo'.
Eroles
Pilar
P
Secci Departamental de Microbiologa, Facultat de Farmcia, Universitat de Valncia, Avgda. V. Andrs Estelles s/n, 46100 Burjassot, Valncia, Spain.
Sentandreu
Maria
M
Secci Departamental de Microbiologa, Facultat de Farmcia, Universitat de Valncia, Avgda. V. Andrs Estelles s/n, 46100 Burjassot, Valncia, Spain.
Elorza
M Victoria
MV
Secci Departamental de Microbiologa, Facultat de Farmcia, Universitat de Valncia, Avgda. V. Andrs Estelles s/n, 46100 Burjassot, Valncia, Spain.
Sentandreu
Rafael
R
Secci Departamental de Microbiologa, Facultat de Farmcia, Universitat de Valncia, Avgda. V. Andrs Estelles s/n, 46100 Burjassot, Valncia, Spain.
D000327:Adsorption; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000908:Antibodies, Fungal / Q000276:immunology; D000935:Antifungal Agents / Q000494:pharmacology; D000946:Antigens, Fungal / Q000235:genetics*; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D002451:Cell Compartmentation; D002473:Cell Wall / Q000737:chemistry / Q000276:immunology*; D003600:Cytosol / Q000737:chemistry / Q000201:enzymology / Q000276:immunology; D004926:Escherichia coli; D019084:Fluorescent Antibody Technique, Indirect; D005936:Glucans / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000276:immunology*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000908:Antibodies, Fungal; D000935:Antifungal Agents; D000946:Antigens, Fungal; D005936:Glucans; D018840:HSP70 Heat-Shock Proteins; C020789:papulacandin B; D010751:Phosphopyruvate Hydratase