The murine AIDS virus Gag precursor protein binds to the SH3 domain of c-Abl.
The Pr60gag protein of the murine AIDS (MAIDS) defective virus promotes the proliferation of the infected target B cells and is responsible for inducing a severe immunodeficiency disease. Using the yeast two-hybrid system, we identified the SH3 domain of c-Abl as interacting with the proline-rich p12 domain of Pr60gag. The two proteins were shown to associate in vitro and in vivo in MAIDS virus-infected B cells. Overexpression of Pr60(gag) in these cells led to a detectable increase of the levels of c-Abl protein and to its translocation at the membrane. These results suggest that this viral protein serves as a docking site for signaling molecules and that c-Abl may be involved in the proliferation of infected B cells.
Dupraz
P
P
Laboratory of Molecular Biology, Clinical Research Institute of Montréal, Québec, Canada.
Rebai
N
N
Klein
S J
SJ
Beaulieu
N
N
Jolicoeur
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D016315:Proto-Oncogene Proteins c-abl / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D018909:src Homology Domains
D015683:Gene Products, gag; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D016315:Proto-Oncogene Proteins c-abl