Characterization of a novel rice bZIP protein which binds to the alpha-globulin promoter.
Many plant basic leucine-zipper (bZIP) proteins have been isolated several of which have been shown to play a role in seed-specific gene expression. We isolated a novel bZIP protein (REB) gene encoding 425 amino acid residues from rice endosperm, which is similar to Opaque-2 heterodimerizing protein (OHP) of maize. The gene product, termed REB, contains Pro- and Gly-rich regions at its N terminus, followed by the typical basic and leucine-repeat regions. Recombinant REB binds to the region from -754 to -562 in the alpha-globulin gene promoter, but not to promoters of other major storage genes such as glutelin, prolamin and albumin. The 5' region of the alpha-globulin gene possesses three binding sites for REB, which were determined as GCCACGT(A/C)AG, by using synthetic oligonucleotides. A Super-shift assay using anti-REB antibody suggested that REB is a major DNA-binding protein for the alpha-globulin gene promoter in rice endosperm.
Nakase
M
M
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Japan.
Aoki
N
N
Matsuda
T
T
Adachi
T
T
D000510:Alpha-Globulins / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D002467:Cell Nucleus / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D017343:Genes, Plant; D016350:Leucine Zippers / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry* / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012639:Seeds / Q000235:genetics
D000510:Alpha-Globulins; D050976:Basic-Leucine Zipper Transcription Factors; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D010940:Plant Proteins; C104734:REB protein, Oryza sativa; D011993:Recombinant Fusion Proteins