title: "Megaprimer" method of PCR-based mutagenesis: the concentration of megaprimer is a critical factor.
authors.affiliation: Department of Medical Microbiology, MRC Pneumococcal Diseases Research Unit, School of Pathology, South African Institute for Medical Research, Johannesburg, South Africa. 174ant@chiron.wits.ac.za
authors.forename: A M
authors.initials: AM
authors.lastname: Smith
authors.forename: K P
authors.initials: KP
authors.lastname: Klugman
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D006602:Hexosyltransferases; D008969:Molecular Sequence Data; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D016133:Polymerase Chain Reaction; D013296:Streptococcus pneumoniae / Q000235:genetics; D013698:Templates, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase