Effects of histone acetylation on chromatin structure.
The effect of histone acetylation was monitored on CHO chromatin structure, following the addition of 7 mM Na-butyrate to the cell culture medium. The properties of both control and hyperacetylated chromatins and nuclei were investigated by circular dichroism, ethidium bromide intercalation, differential scanning calorimetry, and affinity chromatography. Our results are compatible with modest but significant alterations in the various levels of chromatin organization, as a result of the charge neutralization of some lysine residues within the N-terminal region of the histonic octamer. Namely, large statistically significant differences do exist in the heat capacity thermograms of native nuclei, where unfolding into single nucleofilament of the highly packed native chromatin superfiber appears associated with acetylation; at the same time CD, EB, and affinity chromatography point to modest but consistent differences in the compactness of isolated nucleosomes and polynucleosomes.
Gavazzo
P
P
Institute of Biophysics, School of Medicine, University of Genoa, Italy.
Vergani
L
L
Mascetti
G C
GC
Nicolini
C
C
D000107:Acetylation; D000818:Animals; D016466:CHO Cells; D002151:Calorimetry; D002843:Chromatin / Q000737:chemistry*; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D006224:Cricetinae; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
D002843:Chromatin; D006657:Histones