title: Differential expression of CD44s and CD44v10 proteins and syndecan in normal and irradiated mouse epidermis.
abstract: The role of the CD44s adhesion molecule, its epithelial isoforms and its relationship to epidermal proteoglycans such as syndecan was studied in normal and irradiated mouse skin. In normal mouse skin, only 10% of basal cells are strongly CD44s-immunopositive, with a cytoplasmic expression pattern. Double-label experiments with the basal cell marker keratin 14 confirmed the epithelial nature of the strongly CD44s-positive cell type in the basal layer. Some spinous keratinocytes and the majority of the remaining basal cells exhibited a weak membranous staining pattern. In contrast, the epithelial isoform, CD44v10, was strongly present in all basal and suprabasal epithelial cells of the epidermis, with a membranous staining pattern. Syndecan was found in the granular layer of the normal epidermis only. After 1 week of daily irradiation, the entire basal cell layer of the epidermis expressed CD44s in the membrane, but with a varying degree of staining intensity. This reactivity spread to the upper spinous layer after 3 weeks of treatment. In hyperproliferative epidermis, there was no difference in the staining patterns between CD44s and CD44v10. The expression of syndecan switched from the granular layer to the basal and lower spinous layers after 2 weeks of daily irradiation. Immunoreactivity for syndecan was also strongly enhanced in the dermis of irradiated samples. The results suggest an important role for syndecan and CD44 in proliferative processes during radiation-induced accelerated repopulation.
authors.affiliation: Department of Radiation Biology, St. Bartholomew's Hospital, London, UK.
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Kasper
authors.forename: A
authors.initials: A
authors.lastname: Bierhaus
authors.forename: S
authors.initials: S
authors.lastname: Langer
authors.forename: I
authors.initials: I
authors.lastname: Peterson
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: K R
authors.initials: KR
authors.lastname: Trott
mesh_terms: D000818:Animals; D002455:Cell Division / Q000502:physiology; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism* / Q000528:radiation effects; D005260:Female; D018960:Hyaluronan Receptors / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D008815:Mice, Inbred Strains; D016253:Microscopy, Immunoelectron; D016612:Paraffin Embedding; D011509:Proteoglycans / Q000096:biosynthesis*; D053667:Syndecans; D014965:X-Rays
chemical_list: D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D053667:Syndecans