Differential gene expression in human mammary carcinoma cells: identification of a new member of a receptor family.
Differential gene expression in mammary carcinoma cell lines MCF-7 and MCF-7ADR was investigated by Differential Display Technique. These two cell lines represent a model system for progression of mammary carcinoma: MCF-7 is estradiol-dependent for growth, estrogen-receptor positive, tamoxifen responsive, vimentin negative, Adriamycin sensitive and not invasive in vitro or in vivo while MCF-7ADR is estradiol-independent for growth, estrogen-receptor negative, tamoxifen resistant, vimentin positive, Adriamycin resistant and invasive in vitro and in vivo. Here we describe the identification of a new receptor expressed exclusively in MCF-7ADR. The receptor covers 157 amino acids with a predicted topology of four transmembrane and two extracellular domains. Additional members of the gene family are: peripheral membrane protein PMP22, epithelial membrane protein EMP-1 and squamous cell differentiation marker CL-20.
Schiemann
S
S
Boehringer Mannheim GmbH, Penzberg, Germany.
Rückels
M
M
Engelholm
L H
LH
Schwirzke
M
M
Brünner
N
N
Weidle
U H
UH
D000595:Amino Acid Sequence; D001483:Base Sequence; D001943:Breast Neoplasms / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000502:physiology; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D008565:Membrane Proteins; D009185:Myelin Proteins; D009363:Neoplasm Proteins; C073687:PMP22 protein, human; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C097051:epithelial membrane protein-1