Cytotoxic effect of sodium nitroprusside on PC12 cells.
To investigate the biochemical mechanism responsible for NO-induced neurotoxicity, the effect of sodium nitroprusside(SNP), a NO-generating agent, on PC12 cells was studied. The cell density was dose-dependently inhibited by SNP. Neuronally differentiated PC12 cells showed a higher resistance to SNP than the undifferentiated cells. The inhibitory effect was enhanced by 8-Br-cGMP, and reduced by methylene blue. However, 8-Br-cGMP alone had no significant cytotoxicity. SNP also inhibited [3H]-thymidine incorporation into the cells in a dose-dependent manner. The dose response curves for reducing cell density and for inhibiting thymidine incorporation, were found to be virtually superimposable. These results suggested that cytotoxicity elicited by NO seemed to be due to inhibition of DNA synthesis in PC12 cells.
Nakamura
Y
Y
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Yasuda
M
M
Fujimori
H
H
Kiyono
M
M
Pan-Hou
H
H
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism / Q000633:toxicity; D009599:Nitroprusside / Q000378:metabolism / Q000633:toxicity*; D016716:PC12 Cells; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D009599:Nitroprusside; C016276:8-bromocyclic GMP; D009569:Nitric Oxide; D006152:Cyclic GMP; D013936:Thymidine