Determination of captopril in plasma by high-performance liquid chromatography for pharmacokinetic studies.
A high-performance liquid chromatographic method is described for the determination of free captopril in human plasma N-Acetyl-L-cysteine (NAC) was used as an internal standard. Plasma samples were immediately derivatized with N-(1-pyrenyl)maleimide (NPM) and stabilized with 11 M HCl. The drug of interest was isolated using a liquid-liquid extraction with ethyl acetate and separation was obtained using a reversed-phase column under isocratic conditions with fluorescence detection. The sample volume was 150 microliters plasma. The intra- and inter-day accuracy and precision, determined as relative error and coefficient of variation respectively, were less than 10%. The lower limit of quantitation, based on standards with acceptable coefficients of variation, was 25 ng/ml. No endogenous compounds were found to interfere. The linearity was assessed in the range of 25-600 ng/ml. This method has been demonstrated to be suitable for pharmacokinetic studies in humans.
Arroyo
C
C
Hospital Sta. Creu i St. Pau, Pharmacy Department, Barcelona, Spain.
López-Calull
C
C
García-Capdevila
L
L
Gich
I
I
Barbanoj
M
M
Bonal
J
J
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D000959:Antihypertensive Agents / Q000097:blood* / Q000493:pharmacokinetics; D002216:Captopril / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes; D006801:Humans; D008301:Maleimides; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D005456:Fluorescent Dyes; D008301:Maleimides; D002216:Captopril; C003944:N-(3-pyrene)maleimide