Molecular cloning and functional expression of a serotonin receptor from Caenorhabditis elegans.
A cDNA encoding a serotonin receptor has been isolated from a Caenorhabditis elegans mixed stage cDNA library. The nematode serotonin receptor, designated 5HT-Ce, was permanently expressed in murine Ltk-cells, where it mediates adenylate cyclase attenuation. Sequence analysis and the pharmacological profiles demonstrate its relatedness not only to Drosophila and Lymnae 5HT receptors but also to mammalian 5HT1a receptors. The 5HT-Ce-gene does not map close to the position of any known serotonergic mutations.
Olde
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Neurogenetics Section, National Institute for Neurological Disorders and Stroke, NIH, Bethesda, MD, USA.
McCombie
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D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D019556:COS Cells / Q000201:enzymology; D017173:Caenorhabditis elegans / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D011985:Receptors, Serotonin / Q000235:genetics* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D012701:Serotonin / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D018076:DNA, Complementary; D011985:Receptors, Serotonin; D012701:Serotonin; D000262:Adenylyl Cyclases