title: [The use of the polymerase chain reaction in analyzing ancient DNAs (exemplified by that of the Enmynveem mammoth)].
abstract: DNA was isolated from the Enmynveem mammoth muscles, and the control region and cytochrome b gene of the mitochondrial genome were analyzed by polymerase chain reaction (PCR). The mammoth DNA was amplified by both the classical PCR (two primer system) and the single-primer PCR (spPCR) resulting in DNA fragments up to 1600 bp long. Restriction analysis of the mitochondrial cytochrome b gene was carried out. Cytochromes b genes in three Elephantidae genera were compared.
authors.forename: B A
authors.initials: BA
authors.lastname: Maliarchuk
authors.forename: M V
authors.initials: MV
authors.lastname: Derevenko
authors.forename: A G
authors.initials: AG
authors.lastname: Lapinskiĭ
authors.forename: L L
authors.initials: LL
authors.lastname: Solovenchuk
mesh_terms: D000818:Animals; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics; D004606:Elephants / Q000235:genetics*; D008969:Molecular Sequence Data; D010163:Paleontology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping / Q000379:methods; D012800:Siberia
chemical_list: D003573:Cytochrome b Group; D017931:DNA Primers; D004272:DNA, Mitochondrial; D004247:DNA
vernacular_title: Ispol'zovanie polimeraznoĭ tsepnoĭ reaktsii v analize drevnikh DNK (na primere Enmynveemskogo mamonta).