Effect of thrombin and PDGF on endothelin production in cultured mesangial cells derived from spontaneously hypertensive rats.
1. Basal endothelin-1 (ET-1) production in mesangial cells of spontaneously hypertensive rats (SHR) was not different from that of Wistar-Kyoto (WKY) rats, although a trend toward increased ET-1 production was observed in these cells of SHR. 2. Thrombin and platelet-derived growth factor (PDGF) stimulated ET-1 production in a concentration-dependent manner in these cells of both rat strains, but thrombin- and PDGF-induced stimulation of ET-1 production were clearly greater in cells of SHR than WKY rats. 3. The protein kinase C (PKC)-activating phorbol ester, phorbol myristate acetate, stimulated ET-1 production in cells of both rat strains, but this stimulation was significantly greater in cells of SHR than in cells of WKY rats. 4. An inactive enantiomer of phorbol ester, 4alpha-PDD, had no effect on the ET-1 production in these cells of both rat strains. 5. Neither thrombin nor PDGF stimulated ET-1 production in PKC-depleted cells of both rat strains.
Ikeda
M
M
First Department of Internal Medicine, Osaka City University Medical School, Japan.
Kohno
M
M
Horio
T
T
Yasunari
K
K
Yokokawa
K
K
Kano
H
H
Minami
M
M
Hanehira
T
T
Fukui
T
T
Takeda
T
T
D000818:Animals; D002478:Cells, Cultured; D016232:Endothelins / Q000096:biosynthesis*; D004789:Enzyme Activation / Q000187:drug effects; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013917:Thrombin / Q000494:pharmacology*
D016232:Endothelins; D010982:Platelet-Derived Growth Factor; D011493:Protein Kinase C; D013917:Thrombin