Electron paramagnetic resonance studies of proteins in ozone-treated erythrocyte membranes.
The sulfhydryl group specific spin labels have been used to study the membrane proteins in the bovine and human erythrocyte membranes treated with ozone. The ratio hw/hs, determined from the respective peak amplitudes of the resulting EPR spectra of 3-maleimido-1-oxyl-2,2,5,5-tetramethylpyrrolidine bound to the ozone treated membranes, was shown to be elevated following an increased ozone concentration. The ratio of the central to high-field peak amplitude h0/h-1, a measure of the rotational correlation time, determined from EPR spectra of ozonized membranes covalently labeled with 4-(iodoacetamido)-1-oxyl-2,2,5, 5-tetramethylpyrrolidine was shown to be diminished. The changes in the erythrocyte membranes treated with ozone, observed for both spin labels, indicate a dose dependent increase in the mobility of membrane proteins.
Wróbel
A
A
Institute of Physics, Technical University of Wrocław, Poland.
Jezierski
A
A
Gomułkiewicz
J
J
D000818:Animals; D002417:Cattle; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects*; D006801:Humans; D008565:Membrane Proteins / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D008565:Membrane Proteins; D013113:Spin Labels; C029910:3-(2-iodoacetamido)-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl; C024516:N-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolidinyl)maleimide; D010126:Ozone