Detection of 13C-labeled metabolites in the in vivo canine heart by B1 insensitive heteronuclear coherent polarization transfer and comparison of signal enhancement with NOE.
A recently developed adiabatic coherent polarization transfer enhancement technique [H. Merkle, H. Wei, M. Garwood, K. Uğurbil. J. Magn. Reson, 99, 480-494 (1992)] was employed to perform 13C spectroscopy in the intact canine heart in vivo during [2-13C]-acetate infusion into the left descending coronary artery, the results were compared with 13C spectra obtained with conventionally employed nuclear Overhauser enhancement. The results demonstrate that both methods can be performed by using surface coils to obtain in vivo 13C spectra and that coherent polarization transfer provides better enhancement than NOE for [2-13C]-acetate but not for short T2 compounds.
Wei
H
H
Department of Radiology, University of Minnesota Medical School, Minneapolis 55455, USA.
Merkle
H
H
Xu
Y
Y
Ellermann
J
J
Sipprell
K
K
Uğurbil
K
K
D000085:Acetates / Q000378:metabolism*; D000818:Animals; D002247:Carbon Isotopes; D002952:Citric Acid Cycle; D004285:Dogs; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009206:Myocardium / Q000378:metabolism*
D000085:Acetates; D002247:Carbon Isotopes