Requirement of cofactors for RXR/RAR-mediated transcriptional activation in vitro.
Using crude in vitro systems, we have previously shown that RXR/RAR heterodimers are able to activate transcription from the RAR beta 2 promoter in a retinoid-dependent manner. Here we demonstrate that cofactors distinct from general transcription factors or receptors are required to mediate retinoic acid-dependent transcription in vitro.
Valcárcel
R
R
European Molecular Biology Laboratory, Gene Expression Programme, Heidelberg, Germany.
Meyer
M
M
Meisterernst
M
M
Stunnenberg
H G
HG
D002457:Cell Extracts; D002474:Cell-Free System; D006367:HeLa Cells; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D000072482:Retinoic Acid Receptor alpha; D047488:Retinoid X Receptors; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019473:Transcription Factors, TFII / Q000302:isolation & purification; D015533:Transcriptional Activation / Q000502:physiology*; D014212:Tretinoin / Q000494:pharmacology
D002457:Cell Extracts; C000607071:RARA protein, human; D018168:Receptors, Retinoic Acid; D011993:Recombinant Fusion Proteins; D000072482:Retinoic Acid Receptor alpha; D047488:Retinoid X Receptors; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C082925:retinoic acid receptor beta; D014212:Tretinoin