Protein kinase C does not mediate the dopamine-dependent modulation of glutamate receptors in retinal horizontal cells of the perch (Perca fluviatilis).
The whole-cell patch-clamp technique was employed to record membrane currents from cultured horizontal cells of the perch (Perca fluviatilis). The cells were voltage clamped and slowly superfused with an extracellular solution containing L-glutamate. The glutamate concentration in the bath was continuously measured with the help of photodiode and a dye which accumulated in the bath together with the agonist. The PKA-activator forskolin mimicked the effect of dopamine and enhanced glutamate-induced currents, while application of the PKC stimulator PMA or the synthetic diacylglycerol analogue OAG had no significant effects on the dose-response curves of glutamate induced-currents. These results may indicate that the modulation of glutamate receptors in fish horizontal cells is not mediated via a PKC-dependent pathway.
Schmidt
K F
KF
Physiologisches Institut der Justus-Liebig-Universität, Glessen, Germany.
D000818:Animals; D018929:Cell Culture Techniques; D005576:Colforsin / Q000494:pharmacology; D004298:Dopamine / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D010471:Perches / Q000378:metabolism*; D011493:Protein Kinase C / Q000502:physiology*; D017470:Receptors, Glutamate / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D017470:Receptors, Glutamate; D005576:Colforsin; D018698:Glutamic Acid; D011493:Protein Kinase C; D004298:Dopamine