Vasodilator effects of C-type natriuretic peptide on cerebral arterioles in rats.
The vasodilator effects of C-type natriuretic peptide (CNP) were investigated in isolated rat cerebral arterioles. CNP caused dose-dependent vasodilation, maximally by 10.0 +/- 2.2% at 10(-6) M. The median effective concentration (EC50) was 5.2 x 10(-10) M. In contrast, atrial natriuretic peptide and B-type natriuretic peptide, other members of the natriuretic peptide family, produced little or no vasodilation. Pretreatment with methylene blue (10(-4) M) abolished CNP-induced vasodilation, whereas pretreatment with NG-monomethyl-L-arginine or indomethacin did not inhibit vasodilation. Thus, CNP is suggested to cause significant vasodilation in cerebral arterioles via a cyclic guanosine monophosphate-dependent mechanism.
Mori
Y
Y
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
Takayasu
M
M
Suzuki
Y
Y
Shibuya
M
M
Yoshida
J
J
Hidaka
H
H
D000818:Animals; D001160:Arterioles / Q000187:drug effects; D001921:Brain / Q000098:blood supply*; D066298:In Vitro Techniques; D020098:Natriuretic Peptide, C-Type; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014665:Vasodilator Agents / Q000494:pharmacology*
D011506:Proteins; D014665:Vasodilator Agents; D020098:Natriuretic Peptide, C-Type