Does receptor-'Ck' deficiency initiate leukaemia?
We have developed a polyclonal monospecific antibody (designated Ab-Ck) which recognises specifically a novel cell surface Receptor-'Ck', having inherent ability to regulate mevalonate pathway that is vital for many cellular functions ranging from growth control to differentiation. By using this 'Ab-Ck' as a probe, the Western blot analysis of protein fractions derived from various types of normal and cancerous cells/tissues revealed not only that this Receptor-'Ck' was ubiquitously present in various human organs, especially adrenal cortex, aorta, liver and brain, but also that leukaemic cell lines as well as lymphocytes from CML patients were specifically and selectively deficient in this receptor. Based upon these observations, we propose that leukaemic process may be initiated as a consequence of the deficiency of this novel Receptor-'Ck'.
Kaul
D
D
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Singh
J
J
D000906:Antibodies; D000918:Antibody Specificity; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000648:ultrastructure; D005260:Female; D005909:Glioblastoma / Q000648:ultrastructure; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000209:etiology*; D008099:Liver / Q000648:ultrastructure; D010051:Ovarian Neoplasms / Q000648:ultrastructure; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis* / Q000172:deficiency* / Q000276:immunology; D011973:Receptors, LDL / Q000032:analysis* / Q000172:deficiency* / Q000276:immunology; D014407:Tumor Cells, Cultured
D000906:Antibodies; D011973:Receptors, LDL; D020794:Receptor Protein-Tyrosine Kinases