In vitro assessment of the influence of different leucocyte count lavaged from normal and inflamed cow uterus on the mouse embryo development.
The aim of this study was to evaluate the effect of different leucocyte count from normal and inflamed uterus in excess of PMNs on the development of mouse embryos during the morula or blastocyst stages in vitro. Results showed that cultivation of blastocysts in the presence of leucocytes washed from the uterus, after mediation of specific or nonspecific endometritis at a concentration of 1 x 10(4), led to significant inhibition of embryo development. No similar observations were made with blastocysts cultivated in the presence of leucocytes obtained from the normal uterus at 2-4 days after spontaneous ovulation.
Kluciński
W
W
Department of Internal Medicine, Faculty of Veterinary Medicine, Warsaw Agricultural University, Poland.
Sitarska
E
E
Dembele
K
K
Winnicka
A
A
Sikora
J
J
Kleczkowski
M
M
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology* / Q000503:physiopathology; D002454:Cell Differentiation / Q000502:physiology; D018920:Coculture Techniques / Q000379:methods / Q000662:veterinary; D005314:Embryonic and Fetal Development / Q000502:physiology*; D004716:Endometritis / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary*; D005260:Female; D007958:Leukocyte Count / Q000662:veterinary; D007962:Leukocytes / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D007507:Therapeutic Irrigation / Q000379:methods / Q000662:veterinary; D013997:Time Factors; D014599:Uterus / Q000166:cytology* / Q000473:pathology* / Q000502:physiology