Differentiation between bacterial species and sub-species by pyrolysis mass spectrometry of extracted DNA.
Whole cell samples from cultures of Staphylococcus aureus, S. hominis, S. epidermidis and Streptococcus pyogenes were analysed by pyrolysis mass spectrometry and the results were compared with Py-MS-derived analyses of samples of DNA extracted from the same organisms. Py-MS analysis differentiated the four organisms in both circumstances. These results challenge previous assumptions that Py-MS is restricted to detecting phenotypic differences, although the basis for the differentiation of the DNA extracts has yet to be determined.
Mathers
K
K
Department of Microbiology, Medical School, Newcastle-upon-Tyne, UK.
Freeman
R
R
Sisson
P R
PR
Lightfoot
N F
NF
D004269:DNA, Bacterial / Q000032:analysis*; D013058:Mass Spectrometry; D013211:Staphylococcus aureus / Q000145:classification*; D013212:Staphylococcus epidermidis / Q000145:classification*; D013297:Streptococcus pyogenes / Q000145:classification*
D004269:DNA, Bacterial