Salazosulfapyridine suppresses chondrocyte mediated degradation induced by interleukin 1beta.
OBJECTIVE
To investigate the effects of salazosulfapyridine (SASP) and methotrexate (MTX) on interleukin (IL)-1beta treated rabbit chondrocytes.


METHODS
Normal rabbit chondrocytes were cultured to confluency. IL-1beta was added to serum-free culture medium in the presence or absence of SASP or MTX. After 2 days' incubation, the effects were evaluated from the responses of metalloproteinases, glycosaminoglycan (GAG), and prostaglandin E2 (PGE2).


RESULTS
SASP and MTX suppressed GAG and collagenase release into the culture medium from IL-1beta stimulated rabbit chondrocytes in a dose dependent manner. Only SASP suppressed stromelysin and PGE2 release.


CONCLUSIONS
SASP may have a protective effect on cartilage degradation of patients with rheumatoid arthritis.
Nose
M
M
Central Research Laboratories, Santen Pharmaceutical Co. Ltd., Osaka, Japan.
Sasano
M
M
Kawashima
Y
Y
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D015232:Dinoprostone / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D007719:Knee Joint; D008666:Metalloendopeptidases / Q000378:metabolism; D008727:Methotrexate / Q000494:pharmacology; D011817:Rabbits; D012460:Sulfasalazine / Q000494:pharmacology*
D006025:Glycosaminoglycans; D007375:Interleukin-1; D012460:Sulfasalazine; D008666:Metalloendopeptidases; D015232:Dinoprostone; D008727:Methotrexate