Bovine erythrocyte haemolysates enhance plasminogen activation by tissue-type plasminogen activator.
An active fraction that accelerates plasminogen activation by tissue-type plasminogen activator (t-PA) was purified from a haemolysate of bovine erythrocytes. When the haemolysate was mixed with t-PA, it produced a 2- to 3-fold increase in plasminogen activation as measured by an insoluble fibrinolytic assay system and a soluble amidolytic assay system with the chromogenic substrate S-2251. Zymographic analysis showed that, while the haemolysate increased t-PA activity, it did not alter the electrophoretic characteristics of the t-PA nor did it induce any fibrinolysis in the absence of t-PA or plasminogen. The haemolysate was devoid of plasmin and plasminogen activator activity but was most effective in accelerating plasminogen activation by t-PA in the presence of substrate. Based on the purification characteristics of the active fraction in the haemolysate, it appears to have a molecular weight of less than 10 kDa.
Yamada
M
M
Department of Bioresource Development, School of Bioresources, Hiroshima Prefectural University, Japan.
Horiuchi
T
T
Oribe
T
T
Yamamoto
S
S
Sugie
I
I
Gentry
P A
PA
D000818:Animals; D001685:Biological Factors / Q000097:blood* / Q000302:isolation & purification; D002417:Cattle; D002863:Chromogenic Compounds; D004789:Enzyme Activation; D004912:Erythrocytes / Q000378:metabolism*; D005342:Fibrinolysis; D006461:Hemolysis; D007700:Kinetics; D008970:Molecular Weight; D009842:Oligopeptides / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000302:isolation & purification / Q000378:metabolism*
D001685:Biological Factors; D002863:Chromogenic Compounds; D009842:Oligopeptides; C018788:valyl-leucyl-lysine 4-nitroanilide; D010958:Plasminogen; D010959:Tissue Plasminogen Activator