title: Epidermal growth factor selectively enhances functional enterocyte adaptation after massive small bowel resection.
abstract: After massive small bowel resection, the intestine adapts to compensate. In addition to proliferation, enterocytes also undergo selective functional adaptation. In this study we examined the effect of intraperitoneal administration of epidermal growth factor (EGF) on the expression of the brush border dissacharidase sucrase, the sodium glucose cotransporter (SGLT1), and the sodium-potassium ATPase pump (NaK ATPase) by enterocytes in the remnant intestine after massive small bowel resection. Adult Lewis rats underwent either ileal transection or 70% proximal intestinal resection. These animals were subdivided into groups that received either saline or EGF intraperitoneally for 1 week. Ilea from each group were harvested 4 weeks postoperatively. Enterocytes were separated from these segments by calcium chelation. The total protein from the isolated cells was subjected to Western blot analysis. Administration of EGF to animals that underwent transection did not significantly alter the expression of sucrase, SGLT1, or NaK ATPase. After intestinal resection, the expressions of sucrase and SGLT1 were significantly increased. The combination of EGF administration and intestinal resection resulted in a further increase in SGLT1 expression. The intraperitoneal administration of EGF selectively enhanced the expression of SGLT1 by enterocytes after massive small bowel resection. Administration of EGF to sham-operated animals did not have similar effects. These results suggest that EGF augments the adaptive response and may therefore have a therapeutic role in the management of patients with short bowel syndrome.
authors.affiliation: Department of Surgery, Sepulveda Veterans Administration Medical Center, Los Angeles, California, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Dunn
authors.forename: C P
authors.initials: CP
authors.lastname: Parungo
authors.forename: E W
authors.initials: EW
authors.lastname: Fonkalsrud
authors.forename: D W
authors.initials: DW
authors.lastname: McFadden
authors.forename: S W
authors.initials: SW
authors.lastname: Ashley
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D015153:Blotting, Western; D004247:DNA / Q000032:analysis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D007082:Ileum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism / Q000601:surgery*; D008562:Membrane Glycoproteins / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D051269:Sodium-Glucose Transporter 1; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013393:Sucrase / Q000378:metabolism
chemical_list: D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D011506:Proteins; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D004815:Epidermal Growth Factor; D004247:DNA; D013393:Sucrase; D000254:Sodium-Potassium-Exchanging ATPase