title: Molecular cloning of ozone-inducible protein from Pinus sylvestris L. with high sequence similarity to vertebrate 3-hydroxy-3-methylglutaryl-CoA-synthase.
abstract: We have isolated a Pinus sylvestris cDNA encoding a globular protein of 474 amino acids with a predicted molecular weight of 52,995 Da. The deduced amino acid sequence showed 41.9% identity and 13.6% similarity to mammalian cytosolic 3-hydroxy-3-methylglutaryl-CoA-synthase (HMGS). Treatment of Scots pine seedlings with ozone resulted in a transient increase of a 1.95 kb transcript, whereas a 1.2 kb mRNA decreased transiently, indicating a possible influence of ozone on isoprenoid biosynthesis.
authors.affiliation: GSF-National Research Center for Environment and Health, Institute of Biochemical Plant Pathology, Neuherberg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Wegener
authors.forename: W
authors.initials: W
authors.lastname: Gimbel
authors.forename: T
authors.initials: T
authors.lastname: Werner
authors.forename: J
authors.initials: J
authors.lastname: Hani
authors.forename: D
authors.initials: D
authors.lastname: Ernst
authors.forename: H
authors.initials: H
authors.lastname: Sandermann
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000235:genetics*; D008969:Molecular Sequence Data; D010126:Ozone / Q000494:pharmacology*; D041605:Pinus sylvestris; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D018749:RNA, Plant / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C104996:ozone-inducible protein, Pinus sylvestris; D010126:Ozone; D006904:Hydroxymethylglutaryl-CoA Synthase