title: Crenarchaeota in Lake Michigan sediment.
abstract: RNA from Lake Michigan sediment was hybridized with a DNA probe for archaeal 16S rRNA. There was a peak of archaeal rRNA abundance in the oxic zone and another immediately below it. Six contributing species were identified by PCR amplification of extracted DNA with primers specific for archaeal rDNA: two related to Methanosarcina acetivorans and four related to marine crenarchaeotal sequences. rRNA quantification using a DNA probe specific for this crenarchaeotal assemblage showed it is most abundant in the oxic zone, where it accounts for about 10% of total archaeal rRNA.
authors.affiliation: Department of Civil Engineering, Northwestern University, Evanston, Illinois 60201, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: MacGregor
authors.forename: D P
authors.initials: DP
authors.lastname: Moser
authors.forename: E W
authors.initials: EW
authors.lastname: Alm
authors.forename: K H
authors.initials: KH
authors.lastname: Nealson
authors.forename: D A
authors.initials: DA
authors.lastname: Stahl
mesh_terms: D001105:Archaea / Q000302:isolation & purification*; D001483:Base Sequence; D019015:Geologic Sediments; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S