Assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for the presence of bai-like genes.
Eubacterium sp. strain VPI 12708 has several bile acid-inducible (bai) genes which encode enzymes in the bile acid 7 alpha-dehydroxylation (7 alpha DeOH) pathway. Twelve 7 alpha DeOH-positive intestinal bacterial strains were assayed for 7 alpha DeOH activity, and 13 strains were tested for hybridization with bai genes. Cholic acid 7 alpha DeOH activity varied greatly (> 100-fold) among these strains. Southern blot experiments showed that DNA prepared from 7 of 13 strains hybridized with at least one of the bai genes from Eubacterium sp. strain VPI 12708.
Doerner
K C
KC
Department of Microbiology and Immunology, Virginia Commonwealth University-Medical College of Virginia, Richmond 23298-0678, USA.
Takamine
F
F
LaVoie
C P
CP
Mallonee
D H
DH
Hylemon
P B
PB
D015139:Blotting, Southern; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism*; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism; D005051:Eubacterium / Q000235:genetics* / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005798:Genes, Bacterial; D006900:Hydroxylation
D002793:Cholic Acids; D019826:Cholic Acid