title: Expression of the GABAA receptor delta subunit is selectively modulated by depolarization in cultured rat cerebellar granule neurons.
abstract: The levels of several GABAA receptor subunit mRNAs increase as cerebellar granule neurons migrate to their adult positions and receive excitatory mossy fiber input. Despite the temporal similarity of these increases in transcript expression in vivo, studies in cultured granule neurons demonstrated that the subunit mRNAs are differentially regulated. To address the possibility that neuronal activity regulates transcript expression, GABAA receptor subunit mRNA levels were assessed in cultured granule neurons grown in chemically defined, serum-free medium containing either nondepolarizing (5 mM) or depolarizing (25 mM) KCl concentrations. Whereas the delta subunit mRNA was almost undetectable in cultures maintained in nondepolarizing medium, an eightfold increase occurred between days 2 and 4 in cultures grown in depolarizing medium. Furthermore, delta subunit transcript expression was reduced by 76 +/- 6% when neurons in depolarizing medium were switched into nondepolarizing medium. The importance of depolarization in the initiation and maintenance of subunit transcript expression in granule neurons was selective for the GABAA receptor delta subunit. These changes in transcript expression involved calcium entry through L-type calcium channels. Nifedipine treatment (1 microM) both reduced intracellular calcium and decreased delta subunit mRNA expression by 79 +/- 4%. Furthermore, inhibition of Ca2+/calmodulin-dependent protein kinases (CaM kinases) by KN-62 (1 microM) also reduced delta subunit transcript expression. These studies demonstrate that KCl-induced depolarization, a condition that mimics the effects of neuronal activity, selectively modulates GABAA receptor delta subunit mRNA expression through a pathway involving calcium entry and activation of a CaM kinase.
authors.affiliation: Departments of Neurosciences, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4965, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Gault
authors.forename: R E
authors.initials: RE
authors.lastname: Siegel
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000502:physiology*; D002614:Chelating Agents / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D008564:Membrane Potentials / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009543:Nifedipine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000737:chemistry / Q000235:genetics*; D013449:Sulfonamides; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D002614:Chelating Agents; D016895:Culture Media, Serum-Free; D004791:Enzyme Inhibitors; D007546:Isoquinolines; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D013449:Sulfonamides; D013779:Tetrodotoxin; C063302:KN 62; D011189:Potassium Chloride; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D009543:Nifedipine; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide