Interleukin-13 inhibits cytokine secretion by blood monocytes from patients with IgA nephropathy.
In this study we have examined the effects of recombinant human interleukin (IL)-13 on peripheral blood monocytes (PBM) from patients with IgA nephropathy (IgAN). Significantly increased spontaneous and lipopolysaccharide (LPS)-stimulated secretion of tumor necrosis factor-alpha (TNF) and IL-8 was determined in PBM cultures of IgAN patients compared to those of normal controls. In the present study, IL-13 inhibited the spontaneous as well as the LPS-stimulated cytokine secretion of PBM in IgAN. Significant inhibitory effect of IL-13 was observed in cultures of PBM from IgAN patients as well as from normal persons. When both LPS and anti-IL-13 antibody were added together to the PBM, a further increase of LPS-enhanced secretion of cytokines occurred. Taken together, these results indicate that IL-13 down-regulates the secretion of TNF and IL-8 from IgAN PBM.
Matsumoto
K
K
Second Department of Internal Medicine, Nihon University School of Medicine, Itabashi-ku, Tokyo, Japan.
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005922:Glomerulonephritis, IGA / Q000276:immunology*; D006801:Humans; D018793:Interleukin-13 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D011239:Prednisolone / Q000494:pharmacology / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D018793:Interleukin-13; D016209:Interleukin-8; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D011239:Prednisolone