Biochemical lesions of platelets stored as concentrates in PVC bags.
Acid-base status of platelet suspension during storage is a measure of the gas permeability of the bag material. To assess the efficacy of the bags available in our market to store platelets, we compared biochemical lesions of platelets stored in an Indian polyvinyl chloride (PVC) triple bag against a Japanese PVC bag standardized for 5 days platelet storage. Platelet concentrates prepared in both control and test PVC bags were stored for 72 h. Two ml samples were drawn 1 h after preparation, and then at 24 h intervals, for analysis. Our data show that the mean pH value in the test bags was maintained above 6.5. However, the CO2 tension was high and O2 tension was low. We also analyzed malondialdehyde (MDA) formation which is a measure of arachidonic acid metabolism, and seemed to be unaffected in stored platelets. Lactate dehydrogenase (LDH) was not released into the plasma excessively and hence significant platelet lysis was absent during storage. Hypotonic shock response (HSR) of platelets stored in both test and control bags was comparable, indicating the possibility of satisfactory post-transfusion recovery.
Krishnan
L K
LK
Thrombosis Research Unit, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Thiruvananthapuram.
Mathai
J
J
Sulochana
P V
PV
Jacob
J
J
Sivakumar
R
R
D001792:Blood Platelets; D001793:Blood Preservation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011143:Polyvinyl Chloride
D011143:Polyvinyl Chloride