Expression of HLA-DR and cytokine genes on interferon-gamma-stimulated human salivary gland cell line.
Stimulation of a cultured human salivary gland (HSG) cell line by interferon (IFN)-gamma leads to HLA-DR gene expression concomitant with inflammatory cytokine genes such as IL-1 beta, tumor necrosis factor (TNF)-alpha, and IL-6 in vitro. IFN-gamma-induced HLA-DR mRNA expression was clearly detected at 2 h after the stimulation, and thereafter its level of gene expression increased until day 7 on HSG cells by reverse transcriptase (RT)-PCR. Immunofluorescence analysis revealed that cytoplasmic HLA-DR immunoreactivity was detected for the first time at 2 days after the stimulation, and its immunoreactivity increased gradually until day 7, while no immunoreactivity with HLA-DP and HLA-DQ was observed at any of the days. In addition, the expression of IL-1 beta, TNF-alpha, and IL-6 on the IFN-gamma-stimulated HSG cells was detected by immunohistochemistry and RT-PCR analysis. These results indicate that human salivary gland cells can be induced to express HLA-DR mRNA by IFN-gamma concomitant with inflammatory cytokine gene expressions such as IL-1 beta, TNF-alpha, and IL-6.
Hamano
H
H
Department of Pathology, Tokushima University School of Dentistry, Japan.
Haneji
N
N
Yanagi
K
K
Ishimaru
N
N
Hayashi
Y
Y
D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D005802:Genes, MHC Class II / Q000276:immunology*; D006684:HLA-DR Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D012468:Salivary Gland Neoplasms; D012469:Salivary Glands / Q000187:drug effects* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D016207:Cytokines; D006684:HLA-DR Antigens; D007371:Interferon-gamma