Apolipoprotein E genotyping and phenotyping in type II diabetes mellitus patients with hypertriglyceridemia.
OBJECTIVE
We investigated the possible effect of apolipoprotein E (apo E) polymorphism on hypertriglyceridemia in type II diabetes mellitus (DM) patients by both apo E genotyping and phenotyping methods.


METHODS
Eighty Korean type II DM patients were evaluated. Restriction isotyping after DNA amplification was used for apo E genotyping. The isoelectric focusing of neuraminidase-treated sera followed by immunoblotting was used for apo E phenotyping.


RESULTS
The concordant rate between apo E genotyping and phenotyping was 96.3%. Apo E genotype frequencies for all 77 concordant cases were as follows: 72.7% for epsilon 3/3; 16.9% for epsilon 3/4; 7.8% for epsilon 2/3; 1.3% for epsilon 2/4, 1.3% for epsilon 4/4; and 0% for epsilon 2/2. There were no significant differences in apo E genotype frequencies between hypertriglyceridemic (n = 42) and normotriglyceridemic (n = 35) groups.


CONCLUSIONS
Our findings could not support the association between apo E polymorphism and hypertriglyceridemia among type II DM patients.
Kim
J H
JH
Department of Clinical Pathology, Yongdong Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.
Lee
E J
EJ
Kwon
O H
OH
D000368:Aged; D000483:Alleles; D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics*; D002784:Cholesterol / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D005838:Genotype; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics / Q000502:physiology
D001057:Apolipoproteins E; D002784:Cholesterol