Iron repressible outer membrane proteins of Moraxella bovis and demonstration of siderophore-like activity.
Moraxella bovis (strain Epp 63), grown in RPMI 1640 medium supplemented with desferrioxamine mesylate (0.05 mg/ml) resulted in cell free culture supernatants with an increased chromeazurol-S response indicating the presence of high affinity iron binding ligand(s). Supernatants of cultures where growth occurred in tryptic soy broth, RPMI 1640, or RPMI 1640-desferrioxamine supplemented with ferrous sulfate (10 micrograms/ml) were negative on the chromeazurol-S test. Growth of M. bovis in RPMI 1640 or RPMI 1640-desferrioxamine medium induced the expression of previously unrecognized outer membrane proteins whose expression was repressed when the medium was supplemented with iron and which were not produced when growth occurred in tryptic soy broth.
Fenwick
B
B
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.
Rider
M
M
Liang
J
J
Brightman
A
A
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D003470:Culture Media; D003676:Deferoxamine / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D008024:Ligands; D016955:Moraxella bovis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D008024:Ligands; C015076:chrome azurol S; D007501:Iron; D003676:Deferoxamine