title: A method to determine the filter matrix in four-dye fluorescence-based DNA sequencing.
abstract: In a previous paper (Yin et al., Electrophoresis 1996, 17, 1143-1150), an automated method for matrix determination in four-dye fluorescence-based DNA sequencing was presented. As a continuation of that work, we have developed an alternative method to estimate the matrix from raw sequence data. The method uses an iterative clustering technique to associate each 4 x 1 data vector with one column of the desired filter matrix, using Kullback's I-divergence as a distance measure. The method requires less preprocessing of the data and less computation than the approach described by Yin et al. (Electrophoresis 1996, 17, 1143-1150). An example demonstrating applicability of the proposed method to Applied Biosystems sequencer data is given.
authors.affiliation: Biomedical Computer Laboratory, Washington University, St. Louis, MO 63130, USA.
authors.forename: W
authors.initials: W
authors.lastname: Huang
authors.forename: Z
authors.initials: Z
authors.lastname: Yin
authors.forename: D R
authors.initials: DR
authors.lastname: Fuhrmann
authors.forename: D J
authors.initials: DJ
authors.lastname: States
authors.forename: L J
authors.initials: LJ
authors.lastname: Thomas
mesh_terms: D000465:Algorithms; D005456:Fluorescent Dyes; D008433:Mathematics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes