title: Chronic granulomatous disease in two children with recurrent infections: family studies using dihydrorhodamine-based flow cytometry.
abstract: We employed a recently published technique, flow cytometry using the cell permeant dye dihydrorhodamine, to analyze families of two patients with X-linked chronic granulomatous disease. The results illustrate the utility of this method in the diagnosis of this serious immunodeficiency disease and also in the identification of carriers.
authors.affiliation: Division of Developmental and Clinical Immunology, University of Alabama at Birmingham, USA.
authors.forename: T P
authors.initials: TP
authors.lastname: Atkinson
authors.forename: G M
authors.initials: GM
authors.lastname: Bonitatibus
authors.forename: R L
authors.initials: RL
authors.lastname: Berkow
mesh_terms: D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D006580:Genetic Carrier Screening; D008040:Genetic Linkage; D006105:Granulomatous Disease, Chronic / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014960:X Chromosome
chemical_list: D005456:Fluorescent Dyes; D013755:Tetradecanoylphorbol Acetate