Cross protective immunity conferred by a marker-free aroA mutant of Pasteurella multocida.
The aroA gene from Pasteurella multocida serotype A:1 (X-73) was cloned by complementation of the Escherichia coli aroA mutant AB2829 with a DNA library constructed in pUC18. The cloned aroA gene was inactivated by deletion of a 300 bp internal sequence and reintroduced by homologous recombination into the chromosome of X-73 and P-1059 (serotype A:3) using a Pasteurella-E. coli shuttle vector pPBA1100. By subjecting the transformed cells to repeated subculturing in the presence of antibiotic selection coupled with auxotrophic enrichment, marker-free aroA mutants of X-73 and of P-1059 were isolated and designated PMP1 and PMP3, respectively. PMP1 and PMP3 were highly attenuated and capable of conferring complete protection against subsequent lethal challenge infection in a mouse model. Moreover, PMP3-immunized mice were protected against heterologous challenge infection with serotype A:1 or A:4.
Homchampa
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Department of Microbiology, Monash University, Clayton, Australia.
Strugnell
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Adler
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D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases; D000818:Animals; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D015415:Biomarkers; D003001:Cloning, Molecular; D003429:Cross Reactions; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation / Q000276:immunology*; D010326:Pasteurella Infections / Q000276:immunology / Q000517:prevention & control*; D016979:Pasteurella multocida / Q000235:genetics* / Q000276:immunology*; D017384:Sequence Deletion / Q000276:immunology; D014166:Transferases / Q000235:genetics / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014774:Virulence
D001428:Bacterial Vaccines; D015415:Biomarkers; D014613:Vaccines, Attenuated; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase