Molecular cloning and in vitro expression of C. elegans and parasitic nematode ionotropic receptors.
The free living nematode, C. elegans is understood at a level of detail equalled by few other organisms, and much of the cell biology and sequence information is proving of considerable utility in the study of parasitic nematodes. Already, C. elegans provides a convenient vehicle for investigating anthelmintic drug action and resistance mechanisms. Among the ionotropic receptors, with their important roles in the behaviour and development of the organism, are targets for anthelmintics. The subunits of nicotinic acetylcholine receptors of C. elegans form a large and diverse multigene family. Members of this family are among the 11 genes associated with resistance to the anthelmintic drug levamisole.
Fleming
J T
JT
Massachusetts General Hospital Cancer Center, Charlestown 02129, USA.
Baylis
H A
HA
Sattelle
D B
DB
Lewis
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics; D003001:Cloning, Molecular; D018509:DNA, Helminth; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics; D009348:Nematoda / Q000235:genetics / Q000378:metabolism; D017459:Receptors, Amino Acid / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D018079:Receptors, GABA / Q000235:genetics; D017470:Receptors, Glutamate / Q000235:genetics; D017981:Receptors, Neurotransmitter / Q000737:chemistry / Q000235:genetics*; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000235:genetics; D019837:Ryanodine Receptor Calcium Release Channel; D017386:Sequence Homology, Amino Acid
D015220:Calcium Channels; D018509:DNA, Helminth; C506926:ITPR1 protein, human; D053496:Inositol 1,4,5-Trisphosphate Receptors; D009124:Muscle Proteins; D017459:Receptors, Amino Acid; D018160:Receptors, Cytoplasmic and Nuclear; D018079:Receptors, GABA; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D011978:Receptors, Nicotinic; D019837:Ryanodine Receptor Calcium Release Channel