title: Angiotensin type-1 (AT1) receptor gene expression in primarily cultured human arterial umbilical endothelial cells.
abstract: The aim of this study was to investigate the interaction of angiotensin II (Ang II) and human umbilical arterial endothelial cells (HUAEC). Specific binding of 125I-ANG II to primarily cultured HUAEC showed a K(D) of 1.98 +/- 0.53 x 10(-9) M (n = 5) with a maximum binding site of 2.84 +/- 1.07 x 10(-13) mol/mg protein (n = 5). In later passages (third and fifth subculture), this binding site was no longer detectable. Gene expression analysis revealed a strong expression of the angiotensin type-I receptor (AT1-R) in the primarily cultured HUAEC, with a decrease in additional passages. In primarily cultured HUAEC, Ang II (10(-10)-10(-6) M) induced a concentration-dependent increase in intracellular free calcium ([Ca2+]i) that could be blocked by a preincubation with candesartan (TCV-112) but not by PD123319. These data show the expression of the AT1-R in primary cultures of HUAEC. The Ang II-induced increase in [Ca2+]i seems to be mediated by this receptor.
authors.affiliation: Medizinische Universitáts-Poliklinik, Bonn, Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Ko
authors.forename: B
authors.initials: B
authors.lastname: Glodny
authors.forename: S
authors.initials: S
authors.lastname: Stier
authors.forename: G
authors.initials: G
authors.lastname: Totzke
authors.forename: G
authors.initials: G
authors.lastname: Nickenig
authors.forename: R
authors.initials: R
authors.lastname: Düsing
authors.forename: A
authors.initials: A
authors.lastname: Sachinidis
authors.forename: H
authors.initials: H
authors.lastname: Vetter
mesh_terms: D000804:Angiotensin II / Q000378:metabolism* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D011945:Receptors, Angiotensin / Q000235:genetics*; D014469:Umbilical Arteries
chemical_list: D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D002118:Calcium