Decreased expression of the very low density lipoprotein receptor mRNA in the cardiac ventricle of spontaneously hypertensive rats.
1. To elucidate the functional implication of very low density lipoprotein (VLDL) receptor, we studied the gene expression of VLDL receptor in rats. The VLDL receptor mRNA was highly expressed in the cardiac ventricle and skeletal muscle. Intermediate amounts of VLDL receptor mRNA were detected in adipose tissue, adrenal gland, brain and lung. Thus the tissue distribution of VLDL receptor mRNA in rats was similar to that reported previously in rabbits. 2. We studied the gene expression of the VLDL receptor in the heart of stroke-prone spontaneously hypertensive rats (SHRSP), an animal model for hypertension-induced cardiac hypertrophy. RNase protection assay showed that the level of ventricular VLDL receptor mRNA was already decreased to one half when hypertension was not fully developed, and further diminished to one fifth when cardiac hypertrophy was established. 3. It is reported that energy utilization in SHRSP hypertrophied myocardium is impaired. Our results suggest that inactive fatty acid metabolism in the ventricle of SHRSP is related to the lowered expression of the VLDL receptor which is postulated as a gate for triglyceride-rich lipoprotein particle.
Jingami
H
H
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Japan.
Masuzaki
H
H
Matsuoka
N
N
Nakagawa
O
O
Ogawa
Y
Y
Mizuno
M
M
Yamamoto
T
T
Nakao
K
K
D000818:Animals; D015152:Blotting, Northern; D006332:Cardiomegaly / Q000378:metabolism; D006352:Heart Ventricles / Q000201:enzymology / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000096:biosynthesis; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017208:Rats, Wistar; D018110:Receptors, Lipoprotein / Q000096:biosynthesis*; D012260:Ribonucleases / Q000378:metabolism
D008079:Lipoproteins, VLDL; D012333:RNA, Messenger; D018110:Receptors, Lipoprotein; D012260:Ribonucleases; D008071:Lipoprotein Lipase