title: The Leu-enkephalin-encoding sequence DNA-binding factor (LEF) is the transcription factor YY1.
abstract: The Leu-enkephalin-encoding sequence DNA-binding factor (LEF) with high affinity for the Leu-enkephalin-encoding sequences in the prodynorphin and proenkephalin genes has earlier been identified. This factor is composed of three subunits of about 60, 70 (the major DNA-binding subunit), and 95 kDa, respectively. Estimated molecular mass, sequence specificity of DNA-binding, and supershift/inhibition with specific antibodies in a band shift assay showed that the DNA-binding subunit of LEF is identical to the multifunctional transcription factor YY1. However, an antibody against the C-terminus of YY1 distinguished the YY1 complexes with a Leu-enkephalin-encoding sequence and canonical YY1 binding site oligonucleotides, suggesting different protein conformations in complexes with these two DNA fragments.
authors.affiliation: Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden. georgy.bakalkin@knv.ki.se
authors.forename: G
authors.initials: G
authors.lastname: Bakalkin
authors.forename: T
authors.initials: T
authors.lastname: Yakovleva
authors.forename: L
authors.initials: L
authors.lastname: Terenius
mesh_terms: D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004399:Dynorphins / Q000235:genetics; D004723:Endorphins / Q000235:genetics; D004743:Enkephalin, Leucine / Q000235:genetics*; D050981:Erythroid-Specific DNA-Binding Factors; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011487:Protein Conformation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D050986:YY1 Transcription Factor
chemical_list: D004268:DNA-Binding Proteins; D004723:Endorphins; D050981:Erythroid-Specific DNA-Binding Factors; D009838:Oligodeoxyribonucleotides; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494700:Yy1 protein, rat; D004743:Enkephalin, Leucine; D004399:Dynorphins; C036200:rimorphin; D004247:DNA