title: Isolation of the tetrathionate hydrolase from Thiobacillus acidophilus.
abstract: An enzyme capable of hydrolysing tetrathionate was purified from cell-free extracts of Thiobacillus acidophilus. The purified enzyme converts tetrathionate into thiosulfate, sulfur and sulfate. In addition, pentathionate could also be converted by the same enzyme. Measurement of the enzyme activity during purification is based on the absorbance of the initial intermediates formed from tetrathionate in the ultraviolet region, which have not been identified. Enzyme activity could also be measured by the scattering of insoluble sulfur in the visible region. The purified enzyme has a pH optimum of 2.5 and a temperature optimum of 65 degrees C. Enzyme activity is strongly stimulated by the presence of sulfate ions. The purified enzyme is a dimer with two identical subunits of 48 kDa. The ultraviolet-visible absorption spectra and denaturation experiments indicate the presence of an organic cofactor.
authors.affiliation: Kluyver Laboratory of Biotechnology, Department of Microbiology and Enzymology, Delft, The Netherlands.
authors.forename: G A
authors.initials: GA
authors.lastname: De Jong
authors.forename: W
authors.initials: W
authors.lastname: Hazeu
authors.forename: P
authors.initials: P
authors.lastname: Bos
authors.forename: J G
authors.initials: JG
authors.lastname: Kuenen
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D004789:Enzyme Activation; D004790:Enzyme Induction; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis / Q000187:drug effects; D013379:Substrate Specificity; D013431:Sulfates / Q000494:pharmacology; D013696:Temperature; D013776:Tetrathionic Acid / Q000378:metabolism*; D013855:Thiobacillus / Q000201:enzymology* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D013431:Sulfates; D013776:Tetrathionic Acid; D006867:Hydrolases; C104756:tetrathionate hydrolase