title: (+)cis-dioxolane stimulation of cytosolic calcium oscillations and phasic contractions of myometrial smooth muscle.
abstract: These studies sought to determine the intracellular mechanisms underlying muscarinic receptor stimulated phasic contractions of myometrial smooth muscle. Utilizing cytosolic calcium imaging studies with Fura-2 loaded muscle strips, along with in vitro isometric contraction studies, we have demonstrated that phasic myometrial contractions stimulated in response to (+)cis-dioxolane, a muscarinic selective acetylcholine agonist, are mediated by activation of the M3 receptor subtype resulting in stimulation of the phosphatidylinositol signaling pathway and the generation of cytosolic calcium oscillations.
authors.affiliation: Department of Obstetrics & Gynecology, University of Chicago, Illinois 60637, USA.
authors.forename: M
authors.initials: M
authors.lastname: Phillippe
authors.forename: A
authors.initials: A
authors.lastname: Basa
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004148:Dioxolanes / Q000494:pharmacology*; D005260:Female; D018721:Muscarinic Agonists / Q000494:pharmacology*; D009215:Myometrium / Q000378:metabolism*; D009355:Neomycin / Q000494:pharmacology; D010507:Periodicity; D010716:Phosphatidylinositols / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D014590:Uterine Contraction
chemical_list: D004148:Dioxolanes; D018721:Muscarinic Agonists; D010716:Phosphatidylinositols; D011976:Receptors, Muscarinic; D009355:Neomycin; D002118:Calcium; C010962:formal glycol