Effect of polyanions on ferricytochrome c.
Effects of two polyanions (heparin, Nafion) on absorption and CD spectra in the Soret region, and on fluorescence of ferricytochrome c were investigated. The conformational state of ferricyt c in the complexes with studied polyanions is significantly different. Whereas heparin affects the structure in the heme region of ferricyt c only slightly (ferricyt c remains in a native-like state), the influence of Nafion is more expressive (the Met-80-heme iron bond and the deeply buried region around Trp-59 are perturbed). Shift of the pK of the transition from neutral low-spin state to acidic high-spin state to higher pH values was observed.
Sedlák
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Department of Biochemistry, Faculty of Science, P.J. Safárik University, Kosice, Slovakia.
D000818:Animals; D002942:Circular Dichroism; D003574:Cytochrome c Group / Q000737:chemistry*; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006736:Horses; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013054:Spectrophotometry, Atomic
D003574:Cytochrome c Group; D005465:Fluorocarbon Polymers; C040402:perfluorosulfonic acid; D006493:Heparin