title: Flow cytometric platelet enumeration utilizing monoclonal antibody CD42a.
abstract: We investigated a flow cytometric method with monoclonal antibody CD42a as a potential reference method for platelet enumeration by blood count analysers. Using peripheral blood samples from 25 healthy individuals we obtained significant (P < 0.0001) correlations between the results obtained by a FACScan method and similar cell counters (TOA Medical Electronics, Kobe, Japan): r = 0.960 (FACScan vs SSF), r = 0.958 (FACScan vs SE-9000A,B), r = 0.949 (FACScan vs K-4500A) and r = 0.954 (FACScan vs K-4500B). This flow cytometric method for counting platelets using the monoclonal antibody CD42a can be used to check the calibration of the platelet count by blood cell analysers.
authors.affiliation: Scientific Division, R&D, TOA Medical Electronics Co., Ltd, Kobe, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Ishii
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
mesh_terms: D000911:Antibodies, Monoclonal; D003627:Data Interpretation, Statistical; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D010976:Platelet Count; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000276:immunology*; D012015:Reference Standards
chemical_list: D000911:Antibodies, Monoclonal; D019038:Platelet Glycoprotein GPIb-IX Complex