Identification of second malignancies on effusions and fine-needle aspirates using a panel of monoclonal antibodies.
The longer survival of neoplastic patients achieved through improvements of therapeutic regimens has increased the relative risk of developing a second primary tumour (SPT). In this context, conventional cytopathology can define tumour histotype only in a small fraction of cases. In this study, we have evaluated whether selected combinations of monoclonal antibodies (MAbs) to tumour-associated antigens (TAAs) can increase the accuracy of conventional morphology in detecting second primary tumours (SPTs) in two particularly difficult areas of cytodiagnosis, namely that of effusions and pulmonary fine-needle aspirates (FNAs). The immunocytochemical (ICC) analysis of 334 cytological specimens demonstrated that the use of our selected panel of MAbs could allow a more efficient identification of SPTs in comparison with conventional morphology. This diagnostic improvement was statistically significant (P < 0.0001). The present findings show that the immunophenotyping of effusions and FNAs, providing a more accurate and objective identification of SPTs, may have significant therapeutic and epidemiological relevance.
Mottolese
M
M
Regina Elena Cancer Institute, Rome, Italy.
Venturo
I
I
Rinaldi
M
M
Lopez
M
M
Bigotti
G
G
Benevolo
M
M
Natali
P G
PG
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D001202:Ascitic Fluid / Q000473:pathology*; D001707:Biopsy, Needle; D003937:Diagnosis, Differential; D006801:Humans; D016130:Immunophenotyping; D008168:Lung / Q000473:pathology*; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology*; D009362:Neoplasm Metastasis / Q000175:diagnosis; D016609:Neoplasms, Second Primary / Q000276:immunology / Q000473:pathology*; D010996:Pleural Effusion / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm