Transformation of the oomycete Saprolegnia monoïca to hygromycin-B resistance.
Saprolegnia monoïca was stably transformed to hygromycin resistance using the plasmids pTH210 and pHAM34H containing a bacterial phosphotransferase gene fused to regulatory sequences from genes of another Oomycete. Vectors pBT6, pCM54, used to transform higher fungi, yielded no stable transformants. DNA hybridizations indicated that transformation resulted from a single-copy integration of the transforming vector. Development of non-resistant subcultures from transformed colonies revealed that the transgene could become silent and was not uniformly expressed in the transformants.
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Laboratoire de Biologie Cellulaire Fongique, CGMC UMR CNRS 5534, Université Lyon 1, 43, Boulevard 11 Novembre 1918, F-69622 Villeurbanne Cedex, France.
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D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005822:Genetic Vectors; D006921:Hygromycin B / Q000494:pharmacology; D009868:Oomycetes / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013045:Species Specificity; D014170:Transformation, Genetic
D004271:DNA, Fungal; D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; D006921:Hygromycin B; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase