title: Transforming growth factor-beta stimulates bone ongrowth. Hydroxyapatite-coated implants studied in dogs.
abstract: Unloaded cylindrical grit-blasted titanium (Ti-6A-4V) implants (6 x 10 mm) coated with hydroxyapatite ceramic were inserted into the proximal part of the humerus of 20 skeletally mature Labrador dogs. The implants were initially surrounded by a 2 mm gap. In 10 dogs, HA-coated implants without growth factor were inserted in one humerus and implants with 0.3 microgram rhTGF-beta 1 adsorbed onto the HA coating were inserted in the contralateral humerus. In another group of 10 dogs, a dose of 3.0 micrograms rhTGF-beta 1 was tested in a similar design. All dogs were killed at 6 weeks after treatment. Results were evaluated by histomorphometry and mechanical push-out testing. Bone ongrowth was increased by one third, using the 0.3 mg rhTGF-beta 1 stimulation. Bone volume in the gap and mechanical testing showed no statistically significant differences between control and rhTGF-beta 1 stimulated implants. RhTGF-beta 1 only moderately enhanced bone ongrowth to hydroxyapatite-coated implants.
authors.affiliation: Department of Orthopedics, University Hospital of Aarhus, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Lind
authors.forename: S
authors.initials: S
authors.lastname: Overgaard
authors.forename: T
authors.initials: T
authors.lastname: Nguyen
authors.forename: B
authors.initials: B
authors.lastname: Ongpipattanakul
authors.forename: C
authors.initials: C
authors.lastname: Bünger
authors.forename: K
authors.initials: K
authors.lastname: Søballe
mesh_terms: D000497:Alloys; D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000166:cytology; D004285:Dogs; D006811:Humerus; D006882:Hydroxyapatites / Q000494:pharmacology*; D016348:Osseointegration / Q000187:drug effects*; D019736:Prostheses and Implants; D011994:Recombinant Proteins; D014025:Titanium / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D000497:Alloys; D006882:Hydroxyapatites; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C031462:titanium alloy (TiAl6V4); D014025:Titanium