Inhibitory effect of crocetin on benzo(a)pyrene genotoxicity and neoplastic transformation in C3H10T1/2 cells.
Crocetin is a major component in the fruit of Gardenia jasminoides Ellis, a Chinese herbal medicine. In the work, we investigate the protective action and mechanism against benzo(a)pyrene [B(a)P]-induced genotoxicity and neoplastic transformation with a non-toxic dose of crocetin (0.01-0.10 mM) for 1 hour prior to the administration of 0.1 mM B(a)P. B(a)P genotoxicity was inhibited significantly by crocetin in a dose responsive manner. Pretreating C3H10T1/2 cells with crocetin (0.1 mM) also caused a decrease in the covalent binding of B(a)P-diol-epoxide to DNA, to about half that of cells without crocetin treatment. Crocetin also inhibited B(a)P-induced transformations. When the culture was treated with crocetin (0.01, 0.05 and 0.10 mM) for 7 days, the transformation frequencies were lower than that of the culture without crocetin treatment. Furthermore, pretreating cells with crocetin (0.01-0.10 mM) also caused an increase in the activity of GSH S-transferase (GST) to 18-71% that of the cells without crocetin treatment. These results suggest that the inhibition by crocetin of B(a)P-induced genotoxicity and neoplastic transformation in C3H10T1/2 cells is due to a mechanism that increases the activity of GST and decreases the formation of a B(a)P-DNA adduct.
Chang
W C
WC
Department of Medical Technology, China Journal College of Medical Technology, Tainan, Taiwan, Republic of China.
Lin
Y L
YL
Lee
M J
MJ
Shiow
S J
SJ
Wang
C J
CJ
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000494:pharmacology / Q000633:toxicity*; D002338:Carotenoids / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000187:drug effects*; D018736:DNA Adducts / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D005982:Glutathione Transferase / Q000187:drug effects* / Q000378:metabolism; D014801:Vitamin A / Q000031:analogs & derivatives
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D018736:DNA Adducts; C056612:benzo(a)pyrene-DNA adduct; C487773:trans-sodium crocetinate; D014801:Vitamin A; D001564:Benzo(a)pyrene; D002338:Carotenoids; D005982:Glutathione Transferase