Differential expression of alpha 2-adrenoceptor subtypes in purified rat pancreatic islet A- and B-cells.
In the endocrine pancreas, alpha 2-adrenoceptor stimulation reduces glucose-induced insulin secretion from islet B-cells. There is, however, controversy with regard to the effects of alpha 2-agonists on islet A-cell function. In this paper, we have used RNA samples prepared from whole rat islets and from FACS-purified rat A- and B-cells to study alpha 2-adrenoceptor subtype expression. RNase protection assays detected transcripts encoding alpha 2a and alpha 2b subtypes in the RNA pool of rat islets. Reverse transcription-PCR revealed that transcripts for all three alpha 2-adrenoceptor subtypes are present in rat islet cells in purified A-cell RNA. In contrast, RT-PCR of islet B-cell RNA yielded products corresponding to alpha 2a and alpha 2c, with no evidence for the presence of alpha 2b. Thus, the results reveal that both islet cell types express more than one receptor subtype and suggest that the distribution of the subtypes may differ between rat islet A- and B-cells.
Chan
S L
SL
Department of Biological Sciences, University of Keele, Staffs, UK.
Perrett
C W
CW
Morgan
N G
NG
D000818:Animals; D015870:Gene Expression; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012313:RNA; D051381:Rats; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics*; D012260:Ribonucleases / Q000378:metabolism; D014158:Transcription, Genetic
D018341:Receptors, Adrenergic, alpha-2; D012313:RNA; D012260:Ribonucleases