title: Primary structure and properties of the Na+/glucose symporter (Sg1S) of Vibrio parahaemolyticus.
abstract: Previously, we cloned and sequenced a DNA fragment from Vibrio parahaemolyticus and found four open reading frames (ORFs). Here, we clearly demonstrate that one of the ORFs, ORF1, is the gene (sglS) encoding a Na+/glucose symporter (SglS). We characterize the Na+/glucose symporter produced in Escherichia coli mutant (JM1100) cells which lack original glucose transport activity and galactose transport activity. We also show that phlorizin, a potent inhibitor of the SGLT1 Na+/glucose symporter of animal cells, inhibited glucose transport, but not galactose transport, via the SglS system.
authors.affiliation: Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
authors.forename: R I
authors.initials: RI
authors.lastname: Sarker
authors.forename: W
authors.initials: W
authors.lastname: Ogawa
authors.forename: T
authors.initials: T
authors.lastname: Shimamoto
authors.forename: T
authors.initials: T
authors.lastname: Shimamoto
authors.forename: T
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D001426:Bacterial Proteins; D001692:Biological Transport; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames; D010695:Phlorhizin / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000378:metabolism*; D051247:Sodium-Glucose Transport Proteins; D013379:Substrate Specificity; D014736:Vibrio parahaemolyticus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009004:Monosaccharide Transport Proteins; D051247:Sodium-Glucose Transport Proteins; C099811:sodium glucose symporter, Vibrio parahaemolyticus; D012964:Sodium; D010695:Phlorhizin; D005947:Glucose; D005690:Galactose