Expression of MAGE genes in osteosarcoma.
Expression of MAGE genes that encode tumor-rejection antigens recognized by cytotoxic T lymphocytes with major histocompatibility complex class-I antigens was investigated in human osteosarcomas (20 cell lines and eight fresh tumor tissues). MAGE-1, 2, 3, 4, and 6 genes were expressed at the mRNA level in 11 (52.4%), 10 (47.6%), 10 (47.6%) one (4.8%), and 10 (47.6%) of 21 tumor cell lines, respectively, and in five (62.5%), six (75%), five (62.5%), one (12.5%), and five (62.5%) of eight fresh tumor tissues as determined by the reverse transcription-polymerase chain reaction method. MAGE-1 or 4 protein was detected by immunoblot analysis in eight of 11 or one of one tumor cell lines, respectively, where it was expressed at the mRNA level. Major histocompatibility complex class-I antigens were expressed in 19 of 21 tumor cell lines. These results suggest that MAGE tumor-rejection antigens are expressed in substantial numbers of osteosarcomas in a major histocompatibility class-I-restricted manner.
Sudo
T
T
Department of Immunology, Kurume University School of Medicine, Japan.
Kuramoto
T
T
Komiya
S
S
Inoue
A
A
Itoh
K
K
D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics*; D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D058950:Melanoma-Specific Antigens; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000502:physiology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; C072011:MAGEA1 protein, human; C072013:MAGEA3 protein, human; C095204:MAGEA4 protein, human; C095203:MAGEA6 protein, human; C072012:MAGEB2 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D012333:RNA, Messenger