Iron modulation of LPS-induced manganese superoxide dismutase gene expression in rat tissues.
Mitochondrial superoxide dismutase (MnSOD) is usually diminished in cancer cells. We observed that in vivo treatment with LPS produces a strong increase of MnSOD mRNA levels and a weak induction of an inactive protein in rat hepatocarcinomas. In normal liver iron deficiency, obtained with desferrioxamine administration, produces a decrease in the MnSOD induction by LPS, indicating that such induction could depend on tissue iron content. However, no change in MnSOD mRNA has been observed in iron-overloaded tumor tissue. Thus, iron is possibly involved in the transcriptional regulation of the protein, in combination with some other unknown factor that appears to be deficient in tumor cells.
De Leo
M E
ME
Institute of General Pathology, Catholic University, Rome, Italy.
Landriscina
M
M
Palazzotti
B
B
Borrello
S
S
Galeotti
T
T
D000818:Animals; D003676:Deferoxamine / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007501:Iron / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000235:genetics*
D008070:Lipopolysaccharides; D007501:Iron; D013482:Superoxide Dismutase; D003676:Deferoxamine