title: Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients.
abstract: A plasma PCR test, using a nonradioactive PCR plate assay, was evaluated for detection of human cytomegalovirus reactivation. This assay was compared to Southern blotting and found to perform well. As a noncompetitive method of quantitation, it was similar to a competitive method for detecting the number of genome copies per milliliter of plasma in marrow transplant recipients. This is a technically simplified assay with potential for adaptation to automation.
authors.affiliation: Department of Pediatrics, City of Hope National Medical Center, Duarte, California 91010, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: Gallez-Hawkins
authors.forename: B R
authors.initials: BR
authors.lastname: Tegtmeier
authors.forename: A
authors.initials: A
authors.lastname: ter Veer
authors.forename: J C
authors.initials: JC
authors.lastname: Niland
authors.forename: S J
authors.initials: SJ
authors.lastname: Forman
authors.forename: J A
authors.initials: JA
authors.lastname: Zaia
mesh_terms: D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000209:etiology / Q000821:virology*; D015342:DNA Probes / Q000235:genetics; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014773:Virology / Q000379:methods
chemical_list: D015342:DNA Probes; D004279:DNA, Viral