A rapid silver staining and destaining technique for the nucleolus organizer region.
Silver staining of nucleolar organizing regions (NOR) is common, but a standard protocol is lacking. A modification of a rapid silver nitrate staining technique for NORs is presented here. Advantages of the modified technique include reliability, speed, cost and the fact that it can be carried out in the light.
Dhar
P K
PK
Department of Anatomy, Human Genetics Section, Kasturba Medical College, Manipal, Karnataka, India.
Kumar
M R
MR
Nayak
S
S
Rao
T R
TR
Joseph
A
A
Devi
S
S
Kumari
U
U
Bhat
S M
SM
Bhat
K R
KR
D006801:Humans; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D009369:Neoplasms / Q000473:pathology / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D012835:Silver Nitrate / Q000737:chemistry; D016622:Silver Staining / Q000379:methods*
D007202:Indicators and Reagents; D012835:Silver Nitrate; D006861:Hydrogen Peroxide