Manipulating photosynthesis.
The levels of individual photosynthetic proteins can be independently decreased by the Agrobacterium-mediated transformation of plants with antisense RNA constructs. Protocols for the introduction of such constructs into Agrobacterium, the Agrobacterium-mediated transformation of tobacco leaf disks, and the screening and analysis of the transgenic plants produced are described.
Knight
J S
JS
School of Biological Sciences, University of Manchester, UK. Jacqui.Knight@man.ac.i.uk
Madueño
F
F
Barnes
S A
SA
Gray
J C
JC
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D005822:Genetic Vectors; D010788:Photosynthesis; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D016372:RNA, Antisense; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D016372:RNA, Antisense