Modulation of cAMP-dependent protein kinase by derivatives of chondroitin sulfate.
Here, we report investigations about the direct effect of glycosaminoglycans, such as dermatan sulfate, chondroitin 4- and 6-sulfate upon cAMP-dependent protein kinase activity. The results indicate that glycosaminoglycans strongly influence the phosphorylation activity of this enzyme against histone type IIa and [Val6, Ala7]-kemptide. While chondroitin 4-sulfate and dermatan sulfate exhibit inhibitory effects, chondroitin 6-sulfate shows a stimulating effect. In addition, the chondroitin 6-sulfate is also able to reduce the chondroitin 4-sulfate and dermatan sulfate specific inhibition.
Dittmann
J
J
Institut für Physiologische Chemie, Tierärztliche Hochschule Hannover, Germany.
Krischek
C
C
Harisch
G
G
D000818:Animals; D002417:Cattle; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D003871:Dermatan Sulfate / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology; D010766:Phosphorylation
D006657:Histones; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates; D017868:Cyclic AMP-Dependent Protein Kinases