Limitations of alginate gels as a culture model for the study of the effects of UVA radiation on human dermal fibroblasts.
Several studies were undertaken to develop three-dimensional (3-D) cell culture models that allow conditions closer to the in vivo situation. To this end, alginate gels were tested as a 3-D cell culture model that might be useful in the study of the effects of UVA on human dermal fibroblasts. Cell culture in alginate gels and the irradiation conditions were optimized. Results showed that optimized cultures in alginate gels experienced considerable cell death on UVA irradiation compared to the classical monolayer cell culture. Viability tests (cell counting and neutral red assay) were performed to show that only UVA-irradiated alginate gels were responsible for this cytotoxicity. The implication of oxygen species in the phototoxicity induced by ultraviolet light has already been described; for this reason we investigated whether oxygen species were involved in the cytotoxicity induced by alginate upon UVA irradiation. It appeared that superoxide anion is not implicated.
Le Tallec
L P
LP
Laboratoire de Pharmacologie Cellulaire de l'Ecole Pratique des Hautes Etudes, Paris, France.
Korwin-Zmijowska
C
C
Adolphe
M
M
D000464:Alginates / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D005782:Gels; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D007223:Infant; D008875:Middle Aged; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays
D000464:Alginates; D003470:Culture Media; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid