title: Impact of social confrontation on rat CD4 T cells bearing different CD45R isoforms.
abstract: The impact of social defeat on lymphocyte subpopulations and T helper subsets was investigated in Long Evans rats. CD4 T helper cell subsets with distinct functional properties and different cytokine profiles can be distinguished by using the mAbs OX-22 (anti-CD45RC) and OX-7 (anti-CD90, Thy1.1). Male intruders were exposed for 2, 6, or 48 h to aggressive resident pairs. All intruders were attacked upon introduction and were defeated as indicated by frequent display of full submissive postures. After 2 and 48 h of confrontation, drastic but differential effects on blood leukocyte numbers, CD4 and CD8a cells, and CD4 subsets were evident. However, after 6 h of confrontation most lymphocyte subset numbers corresponded to baseline levels. Focusing on CD4 subsets after 2 h of confrontation, we demonstrated that only the number of the CD45RC-CD90(-) subset declines, whereas neither the number of the CD45RC+CD90(-) subset nor the number of the CD45RC-CD90(+) subset (recent thymic emigrants) was influenced. Con A stimulation of sorted subsets identified the CD45RC-CD90(-) as a poor producer of IFN-gamma. The data clearly demonstrate that social factors might differentially influence not only T cell subsets but also T helper cell subsets with distinct cytokine profiles in a possibly time-dependent manner. Such a stress-induced shift toward a CD45RC+CD90(-)-dominated milieu may have important consequences in interpreting results obtained from mitogenic stimulation of blood lymphocytes and cytokine production profiles measured after such a stimulation. In addition, a shift toward a CD45RC+CD90(-) dominance may modify the type and magnitude of immune response, at least temporarily.
authors.affiliation: Norman Cousins Program in Psychoneuroimmunology, UCLA, Los Angeles, California, 90095, USA.
authors.forename: V
authors.initials: V
authors.lastname: Stefanski
authors.forename: G F
authors.initials: GF
authors.lastname: Solomon
authors.forename: A S
authors.initials: AS
authors.lastname: Kling
authors.forename: J
authors.initials: J
authors.lastname: Thomas
authors.forename: S
authors.initials: S
authors.lastname: Plaeger
mesh_terms: D000818:Animals; D002452:Cell Count; D005260:Female; D007962:Leukocytes / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D051381:Rats; D012931:Social Environment; D013601:T-Lymphocytes / Q000378:metabolism*