The fourth component of the sarcoglycan complex.
We found a novel dystrophin-associated protein (DAP) exhibiting almost the same mobility as gamma-sarcoglycan on SDS-PAGE. This novel DAP with basic charge is separated from gamma-sarcoglycan by 2-dimensional PAGE or de-N-glycosylation followed by SDS-PAGE. This DAP is most likely the rabbit homologue of "delta-sarcoglycan", the gamma-sarcoglycan-like protein identified previously [Nigro et al. (1996) Hum. Mol. Genet. 5, 1179-1186], since an internal amino acid sequence from the DAP matched the predicted amino acid sequence of "human delta-sarcoglycan" within the limits of species difference and this DAP was recognized by anti-"delta-sarcoglycan" antibody. The DAP was found to be contained in the sarcoglycan fraction which was prepared by treatment of the dystrophin-DAP complex with n-octyl beta-D-glucoside and crosslinked with beta- and/or gamma-sarcoglycan by a chemical crosslinker, dithiobis(succinimidyl propionate). Therefore, we concluded that the DAP is the fourth component of the sarcoglycan complex.
Yoshida
M
M
Department of Cell Biology, National Institute of Neuroscience, NCNP, Kodaira, Tokyo, Japan. yoshida@ncnaxp.ncnp.go.jp
Noguchi
S
S
Wakabayashi
E
E
Piluso
G
G
Belsito
A
A
Nigro
V
V
Ozawa
E
E
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D003432:Cross-Linking Reagents; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D016189:Dystrophin / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D006868:Hydrolysis; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D011817:Rabbits; D049031:Sarcoglycans; D017386:Sequence Homology, Amino Acid
D003432:Cross-Linking Reagents; D003598:Cytoskeletal Proteins; D016189:Dystrophin; D008562:Membrane Glycoproteins; D049031:Sarcoglycans