title: Influence of anesthetics on the incidence of reperfusion-induced arrhythmias and sudden death in rats.
abstract: We compared the influence of pentobarbital (P, 60 mg/kg), urethane (U, 1.8 g/kg), and a contribution of diazepam with ketamine hydrochloride (D+K, 10 + 100 mg/kg) anesthesia on the incidence of ischemia/ reperfusion-induced arrhythmias in rats. In anesthetized rats, myocardial ischemia was produced by a 6-min ligation of the left main coronary artery, followed by 5 min of reperfusion. The incidence of reperfusion-induced ventricular fibrillation (VF) and ventricular tachycardia (VT) was markedly decreased in the D+K-anesthetized animals compared with the P-anesthetized group (VF, 46 vs. 89%; VT, 64 vs. 94%). The mean blood pressure (MBP) before coronary ligation was significantly lower in U-anesthetized animals (72 +/- 3.5 vs. 102 +/- 4.1 and 108 +/- 5.9 mm Hg in P and D+K, respectively). The MBP recovery was the best in the D+K-anesthetized group. In experimental work, pentobarbitone anesthesia may be recommended for studying arrhythmias, while the combination of diazepam and ketamine may be useful for hemodynamic investigations.
authors.affiliation: Department of Pharmacology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Baczkó
authors.forename: I
authors.initials: I
authors.lastname: Leprán
authors.forename: J G
authors.initials: JG
authors.lastname: Papp
mesh_terms: D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology*; D003645:Death, Sudden / Q000209:etiology*; D003975:Diazepam / Q000494:pharmacology; D006439:Hemodynamics / Q000187:drug effects; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000209:etiology*; D010424:Pentobarbital / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014520:Urethane / Q000494:pharmacology
chemical_list: D000777:Anesthetics; D014520:Urethane; D007649:Ketamine; D010424:Pentobarbital; D003975:Diazepam