Modulations of breast fibroblast and carcinoma cell interactions by a dextran derivative (CMDB7).
Modulation of interactions between human breast carcinoma cells (MCF-7ras) and fibroblasts from normal breast tissue (MG3) and from a post-radiation fibrosis with recurrent breast carcinoma (FPR7) by a dextran derivative (CMDB7) was investigated. In a coculture system, MCF-7ras proliferation was increased (50%) by fibroblasts, and fibroblasts showed a 84% growth increase with MCF-7ras. This co-stimulation did not depend on fibroblast origin. CMDB7 can inhibit the growth of MCF-7ras but not the growth of fibroblasts. The addition of CMDB7 blocks the MCF-7ras stimulation by fibroblasts but not the fibroblast stimulation by MCF-7ras. Fibroblast-Conditioned Medium stimulated over 2-3 fold MCF-7ras's DNA synthesis but CMDB7 did not influence this paracrine stimulation. IGFI, IGFII stimulated MCF-7ras's DNA synthesis whereas bFGF inhibited it. CMDB7 did not block IGFI and IGFII's stimulatory effects but increased bFGF's inhibitory effects. These results indicate that CMDB7 can inhibit fibroblast-breast cancer cell interactions possibly by interfering with paracrine growth factor loops.
Liu
J
J
Laboratoire of Oncologie Cellulaire et Moléculaire, Université Paris 13, Bobigny, France.
Bagheri-Yarmand
R
R
Xia
Y
Y
Crépin
M
M
D001943:Breast Neoplasms / Q000473:pathology*; D002450:Cell Communication / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D018920:Coculture Techniques; D003911:Dextrans / Q000494:pharmacology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D014407:Tumor Cells, Cultured
D003911:Dextrans; D016222:Fibroblast Growth Factor 2