Polyamine contents of human breast cancer cells treated with the cytotoxic agents chlorpheniramine and dehydrodidemnin B.
The cytotoxic agents chlorpheniramine and dehydrodidemnin B decreased the cell growth of estrogen receptor-negative human breast cancer cells MDA-MB231 and estrogen receptor-positive MCF-7, after 48 h treatment. Both agents reduced ornithine decarboxylase activity, but polyamine levels were increased in MDA-MB231 cells treated with dehydrodidemnin B. MCF-7 cells when treated with dehydrodidemnin B showed significant increases in spermidine and spermine contents. The results suggest that besides other effects, the cytotoxicity of DDB could be explained in part by the over-accumulation of spermidine and spermine.
Gómez-Fabre
P M
PM
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
de Pedro
E
E
Medina
M A
MA
Núñez de Castro
I
I
Márquez
J
J
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002744:Chlorpheniramine / Q000494:pharmacology*; D047630:Depsipeptides; D005260:Female; D006801:Humans; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010456:Peptides, Cyclic / Q000494:pharmacology*; D013095:Spermidine / Q000378:metabolism*; D013096:Spermine / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D047630:Depsipeptides; D010456:Peptides, Cyclic; D013096:Spermine; D002744:Chlorpheniramine; D009955:Ornithine Decarboxylase; D013095:Spermidine; C098980:plitidepsin