title: Eimeria tenella: genetic recombination of markers for precocious development and arprinocid resistance.
abstract: A mating was made between two populations of Eimeria tenella possessing the complementary traits of normal development (virulence) + resistance to the anticoccidial drug arprinocid or precocious development (attenuation) + drug-sensitivity. A small number of "recombinant" oocysts was recovered. The inheritance of markers from both parents into 22 cloned lines derived from the recombinant oocysts was confirmed by analyses of restriction fragment length polymorphisms of four repetitive DNA sequences.
authors.affiliation: Institute for Animal Health, Compton Laboratory, Berks, England.
authors.forename: M W
authors.initials: MW
authors.lastname: Shirley
authors.forename: D A
authors.initials: DA
authors.lastname: Harvey
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D003049:Coccidiostats / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000032:analysis; D004351:Drug Resistance / Q000235:genetics; D016786:Eimeria tenella / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005819:Genetic Markers; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic
chemical_list: D003049:Coccidiostats; D016054:DNA, Protozoan; D005819:Genetic Markers; C016245:arprinocid; D000225:Adenine