title: Acetylcholine, outer hair cell electromotility, and the cochlear amplifier.
abstract: The dominant efferent innervation of the cochlea terminates on outer hair cells (OHCs), with acetylcholine (ACh) being its principal neurotransmitter. OHCs respond with a somatic shape change to alterations in their membrane potential, and this electromotile response is believed to provide mechanical feedback to the basilar membrane. We examine the effects of ACh on electromotile responses in isolated OHCs and attempt to deduce the mechanism of ACh action. Axial electromotile amplitude and cell compliance increase in the presence of the ligand. This response occurs with a significantly greater latency than membrane current and potential changes attributable to ACh and is contemporaneous with Ca2+ release from intracellular stores. It is likely that increased axial compliance largely accounts for the increase in motility. The mechanical responses are probably related to a recently demonstrated slow efferent effect. The implications of the present findings related to commonly assumed efferent behavior in vivo are considered.
authors.affiliation: Auditory Physiology Laboratory (The Hugh Knowles Center), Department of Neurobiology, Institute for Neuroscience, Northwestern University, Evanston, Illinois 60208, USA.
authors.forename: P
authors.initials: P
authors.lastname: Dallos
authors.forename: D Z
authors.initials: DZ
authors.lastname: He
authors.forename: X
authors.initials: X
authors.lastname: Lin
authors.forename: I
authors.initials: I
authors.lastname: Sziklai
authors.forename: S
authors.initials: S
authors.lastname: Mehta
authors.forename: B N
authors.initials: BN
authors.lastname: Evans
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D003051:Cochlea / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects*
chemical_list: D000109:Acetylcholine