31P NMR studies of human skin using a modified zig-zag surface coil.
Results are presented from an in vivo study of human skin in which a zig-zag surface coil together with a three-pulse Fourier Series Window (FSW) protocol have been used to obtain minimally contaminated 31P skin spectra of the posterior calf. Phantom experiments indicate that while the fall-off in the B1 field profile of the zig-zag surface coil is superior to conventional surface coil designs, it is still not sufficient on its own to reduce muscle signal contamination to acceptable levels in human studies. The additional spatial localization required is provided by the FSW, which reduces signal contamination to less than 30% of the total signal collected. In a study of 18 normal controls the measured pH was found to be relatively high (7.39 +/- 0.08), while the ratios of skin PCr/ beta-ATP (1.04 +/- 0.35) and PCr/Pi (1.67 +/- 0.4) were found to be low compared with that of skeletal muscle. In addition, substantial signal from phosphomonoesters and phosphodiesters were also observed.
Cowie
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AG
Nuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, UK.
Bastin
M E
ME
Manners
D N
DN
Hands
L J
LJ
Styles
P
P
Radda
G K
GK
D000255:Adenosine Triphosphate / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D005260:Female; D005583:Fourier Analysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010725:Phosphocreatine / Q000378:metabolism; D010758:Phosphorus; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism*
D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate