Epstein-Barr virus (herpesvirus 4) types A, B and herpesvirus-6 variants A and B in patients presenting with recurrent upper respiratory inflammation, persistent paravertebral thoraco-lumbar muscle spasm and fatigue by polymerase chain reaction (PCR).
DNA from Epstein-Barr virus (EBV) Types A, B and Herpesvirus-6 (HHV-6) Variants A and B was detected by the Polymerase chain reaction (PCR) in the saliva of 51 non-immunocompromised donor patients and in the blood of seventy non-immunocompromised donor patients with specific signs and symptoms. The minimum selection criteria for each patient included acute or recurrent upper respiratory infection, unilateral thoracolumbar muscle spasm and fatigue. PCR DNA detection in the saliva of selected donors revealed 80% of the donors had either Type A or B EBV (41 of 51), 34.1% Type B EBV only (14 of 41), 9% Type A only (4 of 41), and 56.1% Type A and B EBV (23 of 41). HHV-6 DNA was detected in 45.0% (23 of 51). PCR for EBV in blood of selected donors revealed 68.5% Type A or B EBV (48 of 70), 0% type B EBV alone, 64.8% Type A EBV only (31 of 48) and 35.4% both Type A and B EBV (17 of 48). HHV-6 was detected in 96.4% (64 of 70). The association of Type B EBV in the pathogenesis of these patients is explored based on the PCR quantitation of B type EBV DNA present in the samples.
McGuire
T
T
McGuire Clinic, Seat Pleasant, Maryland 20743, USA.
Greally
J
J
Leon
J
J
Luka
J
J
D000914:Antibodies, Viral / Q000097:blood; D001416:Back Pain / Q000821:virology; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007958:Leukocyte Count; D018763:Muscle Fatigue; D009128:Muscle Spasticity / Q000821:virology*; D016133:Polymerase Chain Reaction; D012008:Recurrence; D012141:Respiratory Tract Infections / Q000821:virology*; D012463:Saliva / Q000276:immunology / Q000821:virology; D014777:Virus Diseases / Q000175:diagnosis* / Q000276:immunology
D000914:Antibodies, Viral; D004279:DNA, Viral