Effect of fasting on the contractile activity and metabolism of labelled glucose and arachidonic acid in uteri isolated from intact and ovariectomized rat.
The effects of fasting for 4 days on the isometric developed tension (IDT) and on the metabolism of labelled glucose and arachidonic acid in uteri from intact and spayed (25 days) rats, were explored. Starvation produces a fall in the contractile activity of intact rats, while in ovariectomized ones, no differences can be seen with respect to their controls. Fasting produces a fall in the glucose metabolism of both intact and ovariectomized rats, being more noticeable in the former group. Indomethacin (5 x 10(-6) M) increases the metabolism of labelled glucose in all experimental groups, significantly. The metabolism of exogenous arachidonic acid into different eicosanoids, PGE2, PGF2 alpha, 6-keto-F1 alpha and TXB2, shows that total food deprivation diminishes significantly the production of PGE2 in intact rats. In contrast, in ovariectomized starved rats, PGE2 increases markedly. The rest of the metabolites studied are not influenced by fasting. These results show that the effects of fasting on the contractile activity and on the release of some metabolites from arachidonic acid by the uteri isolated from intact rats are not seen in ovariectomized animals.
Casalino-Matsuda
S M
SM
Cátedra de Fisiolgía, Facultad de Odontología, Universidad Nacional de Córdoba, Ciudad Universitaria, Argentina.
Linares
J A
JA
Finkelberg
A B
AB
Goldraij
A
A
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000527:Alprostadil / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001835:Body Weight; D002245:Carbon Dioxide / Q000378:metabolism; D015237:Dinoprost / Q000378:metabolism; D005215:Fasting; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007537:Isometric Contraction / Q000502:physiology; D010052:Ovariectomy; D051381:Rats; D013929:Thromboxane B2 / Q000378:metabolism; D014590:Uterine Contraction / Q000187:drug effects*; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D002245:Carbon Dioxide; D016718:Arachidonic Acid; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D000527:Alprostadil; D005947:Glucose; D007213:Indomethacin