title: Interaction of recombinant rat nucleoside diphosphate kinase alpha with bleached bovine retinal rod outer segment membranes: a possible mode of pH and salt effects.
abstract: An attempt was made to reveal the mode of action of protons and salts on the recently discovered GTP gamma S-dependent interaction of bovine retinal rod outer segments (ROS)1 nucleoside diphosphate kinase (NDP kinase) with the complex between bleached visual receptor rhodopsin and retinal G-protein transducin in bovine ROS membranes. The properties of recombinant rat NDP kinase alpha, that is immunologically similar to the soluble NDP kinase from bovine ROS preparation, have been studied in solution by means of protein fluorescence at different pH and salt concentrations and results were compared with pH and salt effects on the binding of NDP kinase alpha to bleached bovine ROS membranes. The results suggest that NDP kinase alpha itself may serve as a target for protons and salts and mediates their effects on the interaction between the enzyme and ROS membranes.
authors.affiliation: Department of Molecular Biology, Tokyo Metropolitan Institute of Gerontology, Japan.
authors.lastname: Orlov NYa
authors.forename: T G
authors.initials: TG
authors.lastname: Orlova
authors.forename: Y K
authors.initials: YK
authors.lastname: Reshetnyak
authors.forename: E A
authors.initials: EA
authors.lastname: Burstein
authors.forename: N
authors.initials: N
authors.lastname: Kimura
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D009701:Nucleoside-Diphosphate Kinase / Q000187:drug effects / Q000502:physiology*; D011485:Protein Binding; D011522:Protons; D051381:Rats; D011994:Recombinant Proteins; D012243:Rhodopsin / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000187:drug effects / Q000502:physiology*; D012492:Salts / Q000494:pharmacology*; D015293:Transducin / Q000378:metabolism
chemical_list: D011522:Protons; D011994:Recombinant Proteins; D012492:Salts; D012243:Rhodopsin; D009701:Nucleoside-Diphosphate Kinase; D015293:Transducin