title: Multiple promoter elements differentially regulate the expression of the mouse tenascin gene.
abstract: Tenascin (TN) is an extracellular matrix glycoprotein that is expressed in a characteristic spatiotemporal pattern during development and is up-regulated in the adult during tumorigenesis, wound healing, and nerve regeneration. In previous studies, we identified a promoter within the proximal 250 bp upstream of the mouse TN gene that contains several putative regulatory elements that are conserved among vertebrate TN genes. We have identified four different DNA elements within this promoter and show that they contribute in different ways to TN gene expression in NIH 3T3 fibroblasts, C6 glioma cells, and N2A neuroblastoma cells. These elements comprise a binding site for Krox proteins, one for nuclear factor 1, an octamer motif that binds POU-homeodomain proteins, and a novel TN control element. The nuclear factor 1 and TN control element had positive effects on TN promoter activity and formed similar DNA-protein complexes with nuclear extracts from all three cell lines. The Krox element had a negative effect on TN promoter activity in N2A cells, a positive effect in C6 cells, and no effect in NIH 3T3 cells. Two DNA binding complexes, one correlated with the negative and the other with the positive activities of the Krox element, were found to contain the protein Krox24. In cotransfection experiments, the octamer motif was required for induction of TN promoter activity by the POU-homeodomain protein Brn2 in N2A cells but was inactive in C6 cells. Consistent with these findings, N2A cells transfected with Brn2 formed octamer-binding complexes containing N-Oct3, the transcriptionally active form of Brn2, whereas complexes formed in C6 cells contained only N-Oct5A and N-Oct5B. Our results provide a striking example of the diversity of regulatory mechanisms that can be called forth by combining different promoter motifs with transcriptional activators or repressors.
authors.affiliation: Department of Neurobiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Copertino
authors.forename: G M
authors.initials: GM
authors.lastname: Edelman
authors.forename: F S
authors.initials: FS
authors.lastname: Jones
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000235:genetics; D051766:Early Growth Response Protein 1; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018398:Homeodomain Proteins; D017874:Immediate-Early Proteins; D051379:Mice; D008969:Molecular Sequence Data; D025542:Neurofibromin 1; D009687:Nuclear Proteins / Q000378:metabolism; D050809:POU Domain Factors; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011506:Proteins / Q000378:metabolism; D017384:Sequence Deletion / Q000235:genetics; D019063:Tenascin / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D018398:Homeodomain Proteins; D017874:Immediate-Early Proteins; D025542:Neurofibromin 1; D009687:Nuclear Proteins; D050809:POU Domain Factors; D011506:Proteins; D019063:Tenascin; D014157:Transcription Factors; C085076:transcription factor Brn-2