title: Isolation of STLV-I from orangutan, a great ape species in Southeast Asia, and its relation to other HTLV-Is/STLV-Is.
abstract: To study the evolutionary origin of human T-lymphotropic virus type I/simian T-lymphotropic virus type I (HTLV-I/STLV-I), we isolated and characterized STLV-I from orangutans (Pongo pygmaeus). Plasma samples from 3 out of 41 animals examined were reactive by particle agglutination and immunofluorescence, and one of these three was confirmed to be anti-HTLV-I antibody-positive by western blotting (WB). Cultured peripheral blood mononuclear cells from the WB-positive orangutan were reactive to anti-STLV-I-positive rhesus monkey plasma. The proviral long terminal repeat region was amplified by polymerase chain reaction and sequenced. A phylogenetic analysis indicated that orangutan STLV-I is related to the Melanesian group of HTLV-Is and other Asian STLV-Is, but the degree of divergence is considerable.
authors.affiliation: Laboratory of Pathogenic Virus, Institute for Virus Research, Kyoto University, Sakyo-ku.
authors.forename: K
authors.initials: K
authors.lastname: Ibuki
authors.forename: E
authors.initials: E
authors.lastname: Ido
authors.forename: S
authors.initials: S
authors.lastname: Setiyaningsih
authors.forename: M
authors.initials: M
authors.lastname: Yamashita
authors.forename: L R
authors.initials: LR
authors.lastname: Agus
authors.forename: J
authors.initials: J
authors.lastname: Takehisa
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.forename: S
authors.initials: S
authors.lastname: Dondin
authors.forename: M
authors.initials: M
authors.lastname: Hayami
mesh_terms: D000818:Animals; D001483:Base Sequence; D015368:Human T-lymphotropic virus 1 / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011148:Pongo pygmaeus / Q000821:virology*; D015303:Simian T-lymphotropic virus 1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*