Cloning of dnaK and dnaJ homologous genes from a purple non-sulfur bacterium Rhodopseudomonas species.
The dnaK and dnaJ genes were isolated as a cluster from a purple non-sulfur phototrophic bacterium, Rhodopseudomonas species No. 7 by a polymerase chain reaction (PCR) based method. The deduced products of dnaK (631 amino acids) and dnaJ (379 amino acids) were 67% and 56% identical to the respective Escherichia coli gene products. The functions of DnaK and DnaJ could be confirmed by complementation of the respective E. coli mutants.
Momma
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K
Research Institute of Innovative Technology for the Earth, Kyoto, Japan.
Inui
M
M
Yamagata
H
H
Yukawa
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D015183:Restriction Mapping; D012241:Rhodopseudomonas / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C063842:dnaK protein, E coli