title: Role of neutrophils in a rat model of gastric ulcer recurrence caused by interleukin-1 beta.
abstract: The production of several cytokines such as interleukin (IL)-1 in gastric mucosa is increased in subjects infected with Helicobacter pylori, a bacterium associated with ulcer recurrence. This study was performed to determine whether the administration of IL-1 beta can cause recurrence of gastric ulcers in rats. Rats with healed ulcers received an injection of IL-1 beta (0.01 to 1 microgram/kg) or vehicle alone. Some rats received an injection of antiserum to rat neutrophils at the same time as 1 microgram/kg IL-1 beta or an injection of monoclonal antibodies against adhesion molecules (anti-intercellular adhesion molecule-1, anti-CD11a, and anti-CD11b) at 0, 12, and 24 hours after the initial injection. At this dose of IL-1 beta, the numbers of neutrophils and monocytes/macrophages infiltrating the scarred mucosa were higher at 12 and 24 hours than without injection of IL-1 beta. By 48 hours, seven of the eight bealed ulcers in the group treated with 1 microgram/kg IL-1 beta had recurred, as had one of the seven healed ulcers in the group given 0.1 microgram/kg IL-1 beta. No recurrence was found in the rats treated with 0.01 microgram/kg IL-1 beta or vehicle alone. Treatment with antiserum to neutrophils or antibodies to adhesion molecules inhibited both neutrophil infiltration into the scarred mucosa and the ulcer recurrence caused by IL-1 beta. These findings suggest possible mechanisms of recurrence of human peptic ulcers.
authors.affiliation: Third Department of Internal Medicine, Osaka City University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Arakawa
authors.forename: T
authors.initials: T
authors.lastname: Fukuda
authors.forename: K
authors.initials: K
authors.lastname: Higuchi
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D019342:Acetic Acid / Q000008:administration & dosage; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002452:Cell Count; D004724:Endoscopy; D005753:Gastric Mucosa / Q000473:pathology; D006801:Humans; D007274:Injections, Intraperitoneal; D018799:Intercellular Adhesion Molecule-1 / Q000494:pharmacology / Q000502:physiology; D007375:Interleukin-1 / Q000008:administration & dosage / Q000502:physiology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000494:pharmacology / Q000502:physiology; D016177:Macrophage-1 Antigen / Q000494:pharmacology / Q000502:physiology; D008297:Male; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012008:Recurrence; D013047:Specific Pathogen-Free Organisms; D013276:Stomach Ulcer / Q000473:pathology / Q000503:physiopathology*
chemical_list: D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1; D019342:Acetic Acid