title: Immunohistochemical detection of p53 protein in rhabdomyosarcoma: association with clinicopathological features and outcome.
abstract: OBJECTIVE
  Alteration in the p53 tumor suppressor gene is the most common tumor specific genetic change identified in most major cancer types including rhabdomyosarcomas. To investigate the overexpression of p53 and its relation to clinical features and outcome in patients with rhabdomyosarcoma (RMS), an immunocytochemical study was performed.
  
  
  METHODS
  Formalin-fixed paraffin embedded tissue sections obtained from 42 cases of RMS were immunostained with a mouse monoclonal antibody p53-D07. Staining was assessed by evaluating the percentage of p53 immunopositive cancer cell nuclei.
  
  
  RESULTS
  Nuclear accumulation of p53 protein was detected in 8 of 42 (19%) samples. Clinical analyses of patients demonstrated no correlation between positive staining and age, sex, histological subtype, stage and overall survival. This analysis, however, was limited by the small number of patients who demonstrated p53 immunostaining. Nonetheless, a statistically significant association was observed between p53 expression and adverse outcome. Nuclear p53 expression was associated with disease progression or recurrence (p <0.001) and with a worse event free survival (p = 0.0015).
  
  
  CONCLUSIONS
  The nuclear p53 immunoreaction rate is low in RMS, but p53 expression appears to correlate with poor prognosis.
authors.affiliation: Department of Pediatric Oncology, University of Istanbul, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Ayan
authors.forename: O
authors.initials: O
authors.lastname: Dŏgan
authors.forename: R
authors.initials: R
authors.lastname: Kebudi
authors.forename: B
authors.initials: B
authors.lastname: Bavbek
authors.forename: C
authors.initials: C
authors.lastname: Alatli
authors.forename: S
authors.initials: S
authors.lastname: Dervişoğlu
authors.forename: R
authors.initials: R
authors.lastname: Dişçi
authors.forename: M
authors.initials: M
authors.lastname: Demiryont
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D014408:Biomarkers, Tumor; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D051379:Mice; D011379:Prognosis; D012208:Rhabdomyosarcoma / Q000378:metabolism* / Q000503:physiopathology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D016159:Tumor Suppressor Protein p53