title: A routine HPLC method for monitoring midazolam in serum.
abstract: A routine high-performance liquid chromatographic method for measuring midazolam in human serum has been developed. The sample preparation procedure consisted of simple liquid-liquid extraction with dichloromethane, followed by evaporation under nitrogen. The mobile phase used was a mixture of acetonitrile at 0.02 M and sodium acetate at pH 3.0 (80:20, v/v) and a flow-rate of 1.2 mL/min. The separation was performed on two cyanopropyl columns (150 x 4.6 mm). The detection was by UV absorption at 240 nm. A linear range from 10 to 1,000 ng/mL and a quantification limit of 7.4 ng/mL of serum was reached. The mean intra-assay and inter-assay reproducibility from serum sample spiked with 100 ng/mL were 4.1 and 4.7%, respectively. The recoveries from serum sample spiked with 50, 100, 500 ng/mL were 85.46, 85.38 and 85.57%, respectively. This method was developed to allow pharmacokinetics study of midazolam in young patients in short surgical interventions.
authors.affiliation: Department of Clinical Pharmacy, Faculty of Pharmaceutical and Biological Sciences, University Lille II, France.
authors.forename: P
authors.initials: P
authors.lastname: Odou
authors.forename: H
authors.initials: H
authors.lastname: Robert
authors.forename: M
authors.initials: M
authors.lastname: Luyckx
authors.forename: C
authors.initials: C
authors.lastname: Brunet
authors.forename: T
authors.initials: T
authors.lastname: Dine
authors.forename: B
authors.initials: B
authors.lastname: Gressier
authors.forename: M
authors.initials: M
authors.lastname: Cazin
authors.forename: J C
authors.initials: JC
authors.lastname: Cazin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood*; D008752:Methylene Chloride; D008832:Microchemistry; D008874:Midazolam / Q000097:blood*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006993:Hypnotics and Sedatives; D008752:Methylene Chloride; D008874:Midazolam