title: Higher-order organization of subrepeats and the evolution of cervid satellite I DNA.
abstract: Based on sequence analyses of 17 complete centromeric DNA monomers from ten different deer species, a model is proposed for the genesis, evolution, and genomic organization of cervid satellite I DNA. All cervid satellite I DNA arose from the initial amplification of a 31-bp DNA sequence. These 31-bp subrepeats were organized in a hierarchical fashion as 0.8-kb monomers in plesiometacarpalia deer and 1-kb monomers in telemetacarpalia deer. The higher-order repeat nature of cervid centromeric satellite DNA monomers accounts for their high intragenomic and intraspecific sequence conservation. Such high intraspecific sequence conservation validates the use of a single cervid satellite I DNA monomer from each deer species for interspecific sequence comparisons to elucidate phylogenetic relationships. Also, a specific 0.18-kb tandem duplication was observed in all 1-kb monomers, implying that 1-kb cervid satellite I DNA monomers arose from an unequal crossover event between two similar 0.8-kb ancestral DNA sequences.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Lee
authors.forename: D R
authors.initials: DR
authors.lastname: Court
authors.forename: C
authors.initials: C
authors.lastname: Cho
authors.forename: J L
authors.initials: JL
authors.lastname: Haslett
authors.forename: C C
authors.initials: CC
authors.lastname: Lin
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics*; D003670:Deer / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
chemical_list: D004276:DNA, Satellite