title: Effects of nucleotides on cyanide-resistant respiratory activity in mitochondria isolated from antimycin A-treated yeast Hansenula anomala.
abstract: Mitochondria were isolated from Hansenula anomala induced for cyanide-resistant respiration by antimycin A-treatment. Cyanide-resistant respiratory activity in isolated mitochondria was stimulated by AMP, ADP, dAMP, and GMP, but not by ATP, adenosine, cAMP, 2'(3')-AMP, CMP, and UMP. Effects of nucleotides were also observed on cyanide-resistant and salicylhydroxamate-sensitive decylubiquinol oxidase activity. Carbonylcyanide m-chlorophenylhydrazone, oligomycin, and carboxyatractyloside did not affect activation of decylubiquinol oxidase activity by AMP. It is suggested that purine nucleoside 5'-monophosphate or diphosphate stimulates alternative oxidase activity from the outer surface of the mitochondrial inner membrane with a mechanism different from respiratory control. Alternative oxidase activity might be controlled by adenine nucleotides posttranscriptionally in fungi.
authors.affiliation: Department of Biochemistry, Niigata College of Pharmacy, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sakajo
authors.forename: N
authors.initials: N
authors.lastname: Minagawa
authors.forename: A
authors.initials: A
authors.lastname: Yoshimoto
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000968:Antimycin A / Q000494:pharmacology*; D003486:Cyanides / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009711:Nucleotides / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D010843:Pichia / Q000187:drug effects* / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors
chemical_list: D000227:Adenine Nucleotides; D003486:Cyanides; D009711:Nucleotides; D000968:Antimycin A; D006180:Proton-Translocating ATPases; D010100:Oxygen