Effect of vitamin E and indomethacin treatment on adenylate cyclase activity, PGE2 and cAMP levels in murine melanoma cells.
Malignant murine melanoma (BL6) cells cultured in vitro were supplemented with indomethacin (0.15 microM) and varying levels (1-10 micrograms/ml) of vitamin E succinate. The effect of combined indomethacin and vitamin E succinate treatment on the growth as well as the levels of adenylate cyclase activity, prostaglandin E2 (PGE2) and cyclic adenosine monophosphate (cAMP) were determined in these cells. BL6 cells supplemented with 0.15 microM indomethacin and 1-10 micrograms/ml vitamin E succinate showed a significant (P < or = 0.05) decrease in growth at 1 microgram/ml vitamin E succinate, while at 3-10 micrograms/ml, no significant increase or decrease in growth was observed when compared to control cultures (OE). Results from studies of adenylate cyclase activity in BL6 cells showed no significant increase or decrease in enzyme activity, nor were the levels of PGE2 and cAMP affected when the cells were supplemented with 0.15 microM indomethacin and 1-10 micrograms/ml vitamin E succinate.
Ottino
P
P
Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, South Africa.
Duncan
J R
JR
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology*; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D024505:Tocopherols; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D014810:Vitamin E; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D015232:Dinoprostone; D024505:Tocopherols; D007213:Indomethacin