Deletion of Src homology 3 domain results in constitutive activation of Tec protein-tyrosine kinase.
Tec protein-tyrosine kinase (PTK) is the prototype of a new subfamily of non-receptor type PTKs, and is abundantly expressed in hematopoietic tissues. We have revealed that Tec is inducibly tyrosine-phosphorylated and activated by stimulation with a wide range of cytokines. To get more insight into the signaling mechanism through Tec, we have generated a constitutively active form of Tec PTK. Deletion of the Src homology (SH) 3 domain gave rise to a hyperphosphorylated and activated Tec kinase (Tec deltaSH3). The activity of Tec deltaSH3 was confirmed in 293 cells, as well as in cytokine-dependent hematopoietic cells (BA/F3). Tec deltaSH3 should be a useful tool to study the in vivo substrates of Tec PTK.
Yamashita
Y
Y
Department of Molecular Biology, Jichi Medical School, Kawachi-gun, Tochigi.
Miyazato
A
A
Ohya
K
K
Ikeda
U
U
Shimada
K
K
Miura
Y
Y
Ozawa
K
K
Mano
H
H
D000818:Animals; D001665:Binding Sites; D004789:Enzyme Activation; D017353:Gene Deletion; D051379:Mice; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D018909:src Homology Domains
C089848:Tec protein-tyrosine kinase; D011505:Protein-Tyrosine Kinases