Rab proteins in gastric parietal cells: evidence for the membrane recycling hypothesis.
The gastric parietal cell secretes large quantities of HCl into the lumen of the gastric gland in response to secretagogues such as histamine. In the membrane recycling hypothesis, this secretory activity requires the trafficking of the gastric H+/K(+)-ATPase to the cell surface from intracellular tubulovesicles. The Rab subclass of small GTP-binding proteins is thought to confer specificity to vesicle transport throughout the secretory pathway, and previous investigations established that Rab11 is highly expressed in gastric parietal cells. Recent discoveries in intra-Golgi transport and neuronal synaptic vesicle fusion have fortuitously converged on an evolutionarily conserved protein complex involved in vesicle docking and fusion. Recent results indicate that Rab11 is involved in the apical targeting of vesicles in parietal cells and other epithelial cells throughout the gastrointestinal tract. In support of the membrane recycling hypothesis, Rab co-segregates with H+/K(+)-ATPase in parietal cells. The presence of Rab11 on tubulovesicles supports a role for this Rab protein in recycling vesicle trafficking.
Calhoun
B C
BC
Department of Medicine, Medical College of Georgia, Augusta 30912-3175, USA.
Goldenring
J R
JR
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D019204:GTP-Binding Proteins / Q000502:physiology*; D017506:H(+)-K(+)-Exchanging ATPase; D006801:Humans; D010295:Parietal Cells, Gastric / Q000502:physiology*; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
D019204:GTP-Binding Proteins; D017506:H(+)-K(+)-Exchanging ATPase; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins