title: Differential regulation of fast axonally transported proteins during the early response of rat retinal ganglion cells to axotomy.
abstract: We have found that the early response of axotomized rat retinal ganglion cells is characterized by the differential regulation of a number of fast axonally transported proteins. The abundance of 23 radiolabeled fast transported proteins was analyzed at 2 and 5 days after axotomy using two-dimensional gel electrophoresis. Corresponding changes in retinal GAP-43 mRNA were measured using northern analysis. Within 2 days of injury, > 40% of the transported proteins analyzed, including GAP-43, showed increased labeling above control levels. Approximately 13% of transported proteins decreased below control levels, whereas the remainder did not change. Five days after axotomy, only GAP-43 and another fast transported protein, C3, continued to sustain measurable increased labeling above control levels; all previously elevated proteins appeared to have been down-regulated by this time, which corresponds to the onset of cell death. These differential changes were accompanied by parallel increases in GAP-43 mRNA. These results suggest that the molecular changes within rat retinal ganglion cells are differentially regulated within two stages subsequent to damage, initial regenerative growth followed by cell death.
authors.affiliation: Neuroscience Research Laboratories, Florida Atlantic University, Boca Raton 33431-6415, USA.
authors.forename: L
authors.initials: L
authors.lastname: Wodarczyk
authors.forename: V K
authors.initials: VK
authors.lastname: Merrill
authors.forename: G W
authors.initials: GW
authors.lastname: Perry
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D003714:Denervation; D005260:Female; D005470:Fluorometry; D019922:GAP-43 Protein; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D013997:Time Factors
chemical_list: D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger