Characterization of leukotriene production in vivo and in vitro in resident and elicited peritoneal macrophages in chickens and mice.
Previously, we reported differences in arachidonic acid metabolism in elicited chicken peritoneal macrophages when compared with murine resident and elicited peritoneal macrophages. We now describe leukotriene (LT) production in the same systems, using resident (murine) and inflammatory macrophages (from both species). Inflammatory (4- or 42-g Sephadex-elicited) peritoneal macrophages from chickens lacked the capacity to produce LT in vivo (following opsonized zymosan [OZ] stimulation) or in vitro, in response to A23187. In addition, chicken macrophages were unable to metabolize exogenously added LTC4 or LTD4 in vitro. In contrast, resident murine peritoneal macrophages produced measurable quantities of LTs (in vivo) within 5 min with an 8-fold increase after 45 min. LTC4 was effectively converted to LTE4 in vivo in a time-dependent manner (65% LTC4/35% LTE4 after 5 min stimulation with OZ and 6% LTC4/94% LTE4 after 60 min stimulation), but no in vitro. The lack of LTC4 metabolism to LTE4 in vitro could not be explained by cell-cell interaction between adherent and nonadherent cells. LTD4 was not detected under any experimental condition. Murine peritoneal cells incubated with LTD4 (with or without agonist) produced LTE4 in a time-dependent fashion. Addition of L-cysteine (a dipeptidase inhibitor) did not explain the lack of detectable levels of LTD4 following intraperitoneal stimulation with OZ. These results suggest that elicited chicken peritoneal macrophages are incapable of producing LTs compared to murine peritoneal macrophages. In addition, these studies fail to explain the different product profiles with in vivo stimulation of murine peritoneal macrophages as compared to in vitro stimulation.
Whelan
J
J
Lipids Research Laboratory, Cornell University, Ithaca, NY, USA.
Golemboski
K A
KA
Broughton
K S
KS
Kinsella
J E
JE
Dietert
R R
RR
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002645:Chickens; D003545:Cysteine / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D017997:Leukotriene C4 / Q000378:metabolism; D017998:Leukotriene D4 / Q000378:metabolism; D017999:Leukotriene E4 / Q000378:metabolism; D015289:Leukotrienes / Q000378:metabolism*; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015054:Zymosan / Q000494:pharmacology
D003911:Dextrans; D007476:Ionophores; D015289:Leukotrienes; D017997:Leukotriene C4; D000001:Calcimycin; D017998:Leukotriene D4; D017999:Leukotriene E4; D015054:Zymosan; C025614:sephadex; D003545:Cysteine