title: Flow cytometric analysis of lymphocytes in cerebrospinal fluid in patients with tick-borne encephalitis.
abstract: BACKGROUND
  Cerebrospinal fluid (CSF) lymphocyte subsets were examined by flow cytometry in 33 patients with tick-borne encephalitis (TBE) in order to determine their values.
  
  
  METHODS
  Lymphocytes were isolated from CSF and lymphocyte subsets were determined: lymphocytes T (CD3+), lymphocytes B (CD19+), NK cells (CD3-CD56+), helper T cells (CD3+CD4+) and cytotoxic T cells (CD3+CD8+). The expression of IL-2 receptors (CD25+) and transferrin receptors (CD71+) on T cells and HLA-DR molecules on T cell subsets was examined. Furthermore, possible relationships among different TBE patient population variables (gender, age, severity of disease, duration of meningitis) were considered.
  
  
  RESULTS
  The analyses of the CSF lymphocyte population subsets are presented. Lymphocytes T (CD3+) were significantly higher in the CSF than in the peripheral blood as was the case with the T cells that expressed transferrin receptors (CD71). Lymphocytes B (CD19+) and NK cells (CD3-CD56+) prevailed in the peripheral blood. In the early course of the disease, a higher expression of HLA-DR molecules on T lymphocytes was observed, while later a higher expression of IL-2 receptors (CD25+) was observed.
  
  
  CONCLUSIONS
  Significant differences in lymphocyte subsets between the CSF and the peripheral blood were found. Significant time-dependent changes of CSF lymphocyte subsets during course of infection were observed. The results of the present study give us deeper insight into CNS cellular immunopathogenic mechanisms in patients with TBE.
authors.affiliation: Department of Infectious Diseases, University Medical Centre, Ljubljana, Slovenia.
authors.forename: J
authors.initials: J
authors.lastname: Tomazic
authors.forename: A
authors.initials: A
authors.lastname: Ihan
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes; D002469:Cell Separation; D002555:Cerebrospinal Fluid / Q000166:cytology*; D004675:Encephalitis, Tick-Borne / Q000097:blood / Q000134:cerebrospinal fluid*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007694:Killer Cells, Natural; D008297:Male; D013601:T-Lymphocytes
chemical_list: D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M