title: Pharmacokinetic studies on 5-aminolevulinic acid-induced protoporphyrin IX accumulation in tumours and normal tissues.
abstract: Laser-induced fluorescence (LIF) for in vivo point monitoring and fluorescence microscopy incorporating a CCD camera were used to study the fluorescence distribution of 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) in tumours. Fluorescence in a chemically-induced adenocarcinoma in the liver of rats and in an aggressive basal cell carcinoma in a patient were studied after intravenous injection of ALA at a dose of 30 mg/kg body weight. The LIF technique demonstrated slightly more ALA-induced PpIX fluorescence in the tumour than in the surrounding normal liver and abdominal muscle of rats. The visible parts of the human basal cell carcinoma exhibited strong ALA-induced fluorescence, while this fluorescence was much weaker in the necrotic areas of the tumour and in the surrounding normal skin.
authors.affiliation: Department of Surgical Oncology, Norwegian Radium Hospital, Montebello, Oslo, Norway.
authors.forename: H
authors.initials: H
authors.lastname: Heyerdahl
authors.forename: I
authors.initials: I
authors.lastname: Wang
authors.forename: D L
authors.initials: DL
authors.lastname: Liu
authors.forename: R
authors.initials: R
authors.lastname: Berg
authors.forename: S
authors.initials: S
authors.lastname: Andersson-Engels
authors.forename: Q
authors.initials: Q
authors.lastname: Peng
authors.forename: J
authors.initials: J
authors.lastname: Moan
authors.forename: S
authors.initials: S
authors.lastname: Svanberg
authors.forename: K
authors.initials: K
authors.lastname: Svanberg
mesh_terms: D000230:Adenocarcinoma / Q000139:chemically induced / Q000378:metabolism*; D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000818:Animals; D002280:Basal Cell Carcinoma / Q000139:chemically induced / Q000378:metabolism*; D004347:Drug Interactions; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000378:metabolism* / Q000494:pharmacology*; D011524:Protoporphyrins / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D011920:Rats, Inbred WF; D012867:Skin / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX