title: Thermal cycle dideoxy DNA sequencing.
abstract: Thermal cycle dideoxy DNA sequencing eliminates the requirements for independent primer annealing and double-stranded DNA denaturation steps. The method enables sequencing from nanogram amounts of DNA from double-stranded and single-stranded PCR products, and plasmid or phage DNA templates. Thermal cycle sequencing also enables direct sequencing from bacterial colonies or phage plaques. Protocols using the Vent exo- DNA polymerase, helpful suggestions, and a troubleshooting guide are also presented.
authors.affiliation: New England Biolabs, Inc., Beverly, MA 01915, USA.
authors.forename: B E
authors.initials: BE
authors.lastname: Slatko
mesh_terms: D015224:Dideoxynucleosides; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D015224:Dideoxynucleosides