Human megakaryoblastic proliferation and differentiation events observed by phase-contrast cinematography.
Microcinematographic documentation of mitoses, amitoses, endomitoses, or cytoplasmic fusion shortly after completion of mitoses was done in bone marrow specimens of patients with quantitative platelet disorders and controls. In patients with platelet disorders, most mitoses with cell duplication occurred in large promegakaryocytes after 4-fold nuclear and cytoplasmic enhancement. Normal specimens showed polyploidization happening in small megakaryoblasts, while mitoses with cell duplication were seen only after cultivation in freeze-thawed sera of patients with platelet disorders. Frequently, lymphocytes were observed to contact megakaryoblastic cells undergoing mitoses, amitoses and endomitoses and to enter the cytoplasm of megakaryocytes (emperipolesis), leaving it again after several hours.
Boll
I T
IT
Neukölln Hospital, Berlin Free University, Germany.
Domeyer
C
C
Bührer
C
C
D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D006410:Hematopoiesis; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology*; D018715:Microscopy, Video