title: Physical mapping of chromosome 6: a strategy for the rapid generation of sequence-ready contigs.
abstract: The development of radiation hybrid (RH) mapping (Cox et al., 1990) and the availability of large numbers of STS markers, together with extensive bacterial clone resources provided a means to accelerate the process of mapping a human chromosome and preparing bacterial clone contigs ready to sequence. Our aim is to construct physical clone maps covering those regions of chromosome 6 that are not currently extensively mapped, and use these to determine the DNA sequence of the whole chromosome. We report here a strategy which initially involves establishing a high density framework map using RH mapping. The framework markers are then used for the identification of bacterial genomic clones covering the chromosome. The bacterial clones are analysed by restriction enzyme fingerprinting and STS-content analysis to identify sequence-ready contigs. Contig gap closure will also be performed by clone walking.
authors.affiliation: Sanger Centre, Huxton, Cambridge, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Mungall
authors.forename: C A
authors.initials: CA
authors.lastname: Edwards
authors.forename: S A
authors.initials: SA
authors.lastname: Ranby
authors.forename: S J
authors.initials: SJ
authors.lastname: Humphray
authors.forename: R W
authors.initials: RW
authors.lastname: Heathcott
authors.forename: C M
authors.initials: CM
authors.lastname: Clee
authors.forename: C L
authors.initials: CL
authors.lastname: East
authors.forename: E
authors.initials: E
authors.lastname: Holloway
authors.forename: A P
authors.initials: AP
authors.lastname: Butler
authors.forename: C F
authors.initials: CF
authors.lastname: Langford
authors.forename: R
authors.initials: R
authors.lastname: Gwilliam
authors.forename: K M
authors.initials: KM
authors.lastname: Rice
authors.forename: G L
authors.initials: GL
authors.lastname: Maslen
authors.forename: N P
authors.initials: NP
authors.lastname: Carter
authors.forename: M T
authors.initials: MT
authors.lastname: Ross
authors.forename: P
authors.initials: P
authors.lastname: Deloukas
authors.forename: D R
authors.initials: DR
authors.lastname: Bentley
authors.forename: I
authors.initials: I
authors.lastname: Dunham
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D003001:Cloning, Molecular; D016172:DNA Fingerprinting / Q000379:methods; D018076:DNA, Complementary; D015870:Gene Expression; D005819:Genetic Markers; D005822:Genetic Vectors; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D018076:DNA, Complementary; D005819:Genetic Markers