Measurement of tumor oxygenation: a comparison between polarographic needle electrodes and a time-resolved luminescence-based optical sensor.
A novel oxygen sensor which does not rely on electrochemical reduction has been used to measure the oxygenation of the murine sarcoma F in a comparative study with an existing polarographic electrode that is available commercially. The prototype luminescence sensor yielded an oxygen distribution comparable with readings made using a pO2 histograph. The percentage of regions detected that had a pO2 less than 5 mm Hg was 79 and 75 using the Eppendorf pO2 histograph and the luminescence fiber optic sensor, respectively. These values were compatible with a measured radiobiologically hypoxic fraction of 67% in this tumor. The polarographic method detected more regions with a pO2 of 2.5 mm Hg or less (69%) compared with the optical sensor (50%) (P < 0.05). This could reflect differences in the oxygen use of the sensing devices. This initial assessment indicates the potential of a fiber-optic-based oxygen-monitoring system. Such a system should have several advantages including monitoring temporal oxygen changes in a given microregion and use with NMR procedures.
Collingridge
D R
DR
The Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, Middlesex, United Kingdom.
Young
W K
WK
Vojnovic
B
B
Wardman
P
P
Lynch
E M
EM
Hill
S A
SA
Chaplin
D J
DJ
D000818:Animals; D005260:Female; D005456:Fluorescent Dyes; D000860:Hypoxia / Q000175:diagnosis; D008163:Luminescent Measurements; D051379:Mice; D008808:Mice, Inbred CBA; D009942:Organometallic Compounds; D010100:Oxygen / Q000378:metabolism; D010618:Phenanthrolines; D011048:Polarography; D012428:Ruthenium; D012513:Sarcoma, Experimental / Q000098:blood supply* / Q000532:radiotherapy
D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D010618:Phenanthrolines; D012428:Ruthenium; C041132:tris(4,7-diphenyl-1,10-phenanthroline)ruthenium (II); D010100:Oxygen