title: The effect of Iloprost (ZK 36374) on creatine phosphate, adenine nucleotides and superoxide dismutase activity in ischaemic canine myocardium.
abstract: Ischaemic injury was produced in the dog heart by occluding the left anterior descending coronary artery just below the second diagonal branch for the duration of 3 h. A prostacyclin analogue, ZK 36374 (Iloprost) was administered (10 micrograms/kg) just before the coronary artery occlusion. During 3 h occlusion ATP levels in the ischaemic area declined from 65.4 +/- 7.1 mM/g protein in sham-operated group to 31.5 +/- 5.2 microM/g protein in no-drug group and to 44.1 +/- 5.0 microM/g protein in Iloprost group. Creatine phosphate decreased from 112 +/- 14 microM/g protein, to 81 +/- 10 microM/g protein in no-drug group and to 95 +/- 12 microM/g protein in Iloprost group. The energy charge (ATP + 0.5 ADP/ATP + ADP + AMP) decreased slightly but not significantly in no-drug and Iloprost group. Three hours of LAD occlusion produced a significant fall in SOD activity in the ischaemic heart in comparison to the non-ischaemic and Iloprost-treated hearts.
authors.affiliation: Department of Anaesthesiology, Medical Academy, Agricultural University, Lublin, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Ogonowska-Kobusiewicz
authors.forename: W
authors.initials: W
authors.lastname: Kobusiewicz
authors.forename: J
authors.initials: J
authors.lastname: Michalak
authors.forename: A
authors.initials: A
authors.lastname: Ledwozyw
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D016285:Iloprost / Q000494:pharmacology*; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D009206:Myocardium / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D010725:Phosphocreatine / Q000032:analysis* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D000227:Adenine Nucleotides; D014665:Vasodilator Agents; D010725:Phosphocreatine; D013482:Superoxide Dismutase; D016285:Iloprost