DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes.
DNA fragments coding for the ribosomal RNA and the surface array proteins of Campylobacter fetus have been cloned from a genomic library constructed in Escherichia coli. They were used in the molecular characterization of C. fetus (subsp. fetus; subsp. venerealis) strains by restriction fragment length polymorphism (RFLP) method. Ribotyping results showed that all strains of the two subspecies can be classified under one ribogroup implying very close relatedness. The sapA gene DNA marker, however, discriminated all the strains regardless of the subspecies when chromosomal DNA was restricted with HindIII, HaeIII, XbaI or EcoRV. These results illustrate that the sapA probe is potentially useful in fingerprinting C. fetus strains and in determining the relationships of strains for epidemiological purposes.
Denes
A S
AS
Animal Diseases Research Institute Nepean, Ont, Canada.
Lutze-Wallace
C L
CL
Cormier
M L
ML
Garcia
M M
MM
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins; D002169:Campylobacter Infections / Q000175:diagnosis* / Q000662:veterinary*; D002168:Campylobacter fetus / Q000145:classification* / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases; D002876:Chromosomes, Bacterial; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D003937:Diagnosis, Differential; D005260:Female; D015698:Genomic Library; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D012756:Sheep; D012757:Sheep Diseases
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008562:Membrane Glycoproteins; D012335:RNA, Ribosomal; C055764:S-layer proteins; D015247:Deoxyribonuclease HindIII; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases; C024403:GGCC-specific type II deoxyribonucleases