Immunoelectron microscopy shows a clustered distribution of NADPH oxidase components in the human neutrophil plasma membrane.
The NADPH oxidase that produces superoxide in professional phagocytic cells is a flavocytochrome b electron transport chain in the membrane, a heterodimer of gp91phox and p22phox, that is activated by a number of cytosolic proteins, including p47phox, p67phox, and the small GTP-binding protein p21rac, which translocate to the membrane and attach to the flavocytochrome on activation. The components of this oxidase were localized on the cytoplasmic surface of the plasma membrane of adherent unroofed neutrophils by immunolabeling. Components of the NADPH oxidase and p21rac were found together in punctate clusters occupying 0.03-0.1 microm2 of the cytoplasmic surface of the plasma membrane where the density of labeling of the cytosolic components was increased after stimulation with phorbol myristate acetate.
Wientjes
F B
FB
Department of Medicine, University College London, United Kingdom.
Segal
A W
AW
Hartwig
J H
JH
D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000201:enzymology; D003599:Cytoskeleton / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000032:analysis*; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis*; D016253:Microscopy, Immunoelectron; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000737:chemistry*; D009504:Neutrophils / Q000201:enzymology*; D013194:Staining and Labeling; D020744:rac GTP-Binding Proteins
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins