title: [A novel method of subtraction cDNA hybridization allowing cloning of genes showing differential expression in cell micropopulations].
abstract: A novel, efficient and simple technique that combines subtractive hybridization with kinetic enrichment is proposed for obtaining enriched cDNA. The method is based on the use of a set of special primers that allow for the selective amplification by PCR only of differentially distributed sequences. Using the proposed technique, cDNA of a new gene XEp-1, specifically expressed in the presumptive epidermis of Xenopus laevis, was cloned, starting with the stage of midgastrula.
authors.forename: O L
authors.initials: OL
authors.lastname: Vasil'ev
authors.forename: S A
authors.initials: SA
authors.lastname: Luk'ianov
authors.forename: A V
authors.initials: AV
authors.lastname: Beliavskiĭ
authors.forename: O V
authors.initials: OV
authors.lastname: Kazanskaia
authors.forename: A G
authors.initials: AG
authors.lastname: Zaraĭskiĭ
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D005801:Genes, Homeobox; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D014982:Xenopus laevis
chemical_list: D018076:DNA, Complementary
vernacular_title: Novyĭ metod vychitaiushcheĭ gibridizatsii kDNK, pozvoliaiushchiĭ klonirovat' geny, differentsial'no ékspressiruiushchiesia v mikropopuliatsiiakh kletok.