Polysaccharides in fungi. XXXVIII. Anti-diabetic activity and structural feature of a galactomannan elaborated by Pestalotiopsis species.
A fungus of Pestalotiopsis species produced an extracellular, water-soluble polysaccharide (PS-N). PS-N exhibited significant hypoglycemic activity in streptozotocin-induced diabetic mice following intraperitoneal administration and had an effect on oral glucose tolerance in normal mice following oral administration. PS-N ([alpha]D +34.5 degrees in water) was homogeneous on gel chromatography, it is composed of galactose and mannose in a molar ratio of 1:9, and its molecular weight was estimated by gel chromatography to be about 24,000. Its structure was investigated by a combination of chemical and spectroscopic methods. The results indicated that PS-N, a highly branched galactomannan, is composed of beta-(1-->3)-linked D-galactofuranosyl and non-reducing terminal beta-D-galactofuranosyl residues, in addition to alpha-D-mannopyranosyl residues of a yeast mannan type.
Kiho
T
T
Gifu Pharmaceutical University, Japan.
Itahashi
S
S
Sakushima
M
M
Matsunaga
T
T
Usui
S
S
Ukai
S
S
Mori
H
H
Sakamoto
H
H
Ishiguro
Y
Y
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D005690:Galactose / Q000031:analogs & derivatives; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D008297:Male; D008351:Mannans / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D003904:Mitosporic Fungi / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013311:Streptozocin; D013329:Structure-Activity Relationship
D007004:Hypoglycemic Agents; D008351:Mannans; D011134:Polysaccharides; C012990:galactomannan; D013311:Streptozocin; D005690:Galactose