Cyclic ADP-ribose measurements in rat pancreatic islets.
Cyclic ADP-ribose was measured by a radioimmunological procedure in rat pancreatic islets. The cyclic ADP-ribose content of the islets was much lower immediately after isolation (< or = 2.3 +/- 0.2 fmol/islet) than after a further incubation of 90 min in a salt-balanced medium (26.4 +/- 1.3 fmol/islet). Under the latter condition, the islet cyclic ADP-ribose content was not affected by the concentration of D-glucose and/or D-fructose in the incubation medium and, when expressed per micrograms protein, was 3- to 30-fold higher than that found in other biological samples such as liver, whole pancrease or tumoral islet cells. Although these findings might suggest a physiological role for cyclic ADP-ribose in islet cells, they argue against the view that the cyclic nucleotide acts as a coupling factor in the process of hexosestimulated insulin release.
Malaisse
W J
WJ
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Kanda
Y
Y
Inageda
K
K
Scruel
O
O
Sener
A
A
Katada
T
T
D000246:Adenosine Diphosphate Ribose / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D007515:Islets of Langerhans / Q000737:chemistry*; D008099:Liver / Q000737:chemistry; D010306:Parotid Gland / Q000737:chemistry; D051381:Rats; D013997:Time Factors
D000246:Adenosine Diphosphate Ribose