Analysis of the mutagenic potential of ENU and MMS in germ cells of male C57BL/6 lacI transgenic mice.
Mutant frequencies in male germ cells were determined in mice 3 days after exposure to saline, methylmethane sulfonate (MMS), or ethylnitrosourea (ENU). DNA was isolated from seminiferous tubules by a modified version of the drop dialysis method. A 5-fold increase in mutant frequency was observed in mice treated with ENU. No statistically significant increase was observed in mice treated with MMS.
Winegar
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RA
SRI International, Menlo Park, CA 94025, USA. richard_winegar@qm.sri.com
Carr
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Mirsalis
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JC
D000818:Animals; D004247:DNA / Q000187:drug effects / Q000302:isolation & purification; D005038:Ethylnitrosourea / Q000633:toxicity*; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012671:Seminiferous Tubules / Q000166:cytology; D013094:Spermatozoa / Q000187:drug effects*
D009153:Mutagens; D004247:DNA; D008741:Methyl Methanesulfonate; D005038:Ethylnitrosourea