Chronic extracellular acidosis induces plasmalemmal vacuolar type H+ ATPase activity in osteoclasts.
Proton extrusion into an extracellular resorption compartment is an essential component of bone degradation by osteoclasts. Chronic metabolic acidosis is known to induce negative calcium balance and bone loss by stimulating osteoclastic bone resorption, but the underlying mechanism is not known. The present studies were undertaken to evaluate whether chronic acidosis affects proton extrusion mechanisms in osteoclasts cultured on glass coverslips. Acidosis, mimicked experimentally by maintaining the cells at extracellular pH 6.5, rapidly lowered intracellular pH to 6.8. However, after 2 hours, a proportion of cells demonstrated the capacity to restore intracellular pH to near normal levels. To define the mechanism responsible for this recovery, the activity of individual H+ transport pathways was analyzed. We found that chronic acid treatment for up to 6 h did not significantly affect the cellular buffering power or Na+/H+ antiport activity. In contrast, chronic acidosis activated vacuolar H+ pumps in the osteoclasts. Although only approximately 5% of the control cells displayed proton pump activity, about 40% of cells kept at extracellular pH 6. 5 for 4-6 h were able to recover from the acute acid load by means of bafilomycin A1-sensitive proton extrusion. Conversely, the H+-selective conductance recently described in the plasma membrane of osteoclasts was clearly inhibited in the cells exposed to chronic acidosis. Following acid treatment, the activation threshold of the H+ conductance was shifted to more positive potentials, and the current density was significantly reduced. Considered together, these results suggest that induction of plasmalemmal vacuolar type ATPase activity by chronic acidosis, generated either systemically due to metabolic disease or locally at sites of inflammation, is likely to stimulate osteoclastic bone resorption and thus to promote bone loss.
Nordström
T
T
Division of Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8.
Shrode
L D
LD
Rotstein
O D
OD
Romanek
R
R
Goto
T
T
Heersche
J N
JN
Manolson
M F
MF
Brisseau
G F
GF
Grinstein
S
S
D000138:Acidosis / Q000201:enzymology*; D000818:Animals; D001693:Biological Transport, Active; D001862:Bone Resorption; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000502:physiology; D004790:Enzyme Induction; D005110:Extracellular Space / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D010010:Osteoclasts / Q000201:enzymology*; D018408:Patch-Clamp Techniques; D006180:Proton-Translocating ATPases / Q000096:biosynthesis*; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases; D015032:Zinc / Q000502:physiology
D017923:Sodium-Hydrogen Exchangers; D012964:Sodium; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D015032:Zinc