Antibody against synthetic rat PTH peptide (1-34) blocks PTH-mediated cAMP formation and phosphate transport in opossum kidney cells.
A novel antibody against synthetic rat parathyroid hormone (rPTH(1-34)) was successfully produced in rabbits at a titer of 1:3,000. The ability of this antibody to block PTH was studied utilizing the hormone-sensitive cAMP formation and Na(+)-dependent phosphate transport in the opossum kidney (OK) cells. rPTH peptide(1-34) stimulated cAMP formation and inhibited Na(+)-dependent phosphate transport in OK cells in a dose-dependent manner. Treatment of OK cells with the antisera significantly decreased the level of cAMP and attenuated the inhibition of Na(+)-dependent phosphate transport in response to rPTH(1-34) at a dilution of 1:1,000. Nonimmune rabbit sera at the same dilution did not influence these hormone-sensitive effects. We conclude that antibody against synthetic PTH peptide can be used to study the biological activities of this hormone.
Zhang
Z
Z
Department of Pediatrics, University of Mississippi Medical Center, Jackson 39216, USA.
Raj
H G
HG
Reddy
R L
RL
Baliga
R
R
D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D009893:Opossums; D010281:Parathyroid Hormone / Q000502:physiology*; D010710:Phosphates / Q000378:metabolism* / Q000493:pharmacokinetics; D011817:Rabbits; D051381:Rats; D012964:Sodium / Q000378:metabolism; D013268:Stimulation, Chemical; D019379:Teriparatide / Q000276:immunology*
D000906:Antibodies; D010281:Parathyroid Hormone; D010710:Phosphates; D019379:Teriparatide; D012964:Sodium; D000242:Cyclic AMP