Molecular cloning and sequence analysis of the mouse protein C inhibitor gene.
The gene encoding mouse protein C inhibitor (mPCI) was isolated and its nucleotide sequence determined. Alignment of the genomic sequence with that of a cDNA obtained from mouse testis revealed that the mPCI gene (like the human counterpart) is composed of five exons and four introns with highly conserved exon/intron boundaries. It encodes a pre-polypeptide of 405 amino acids, which shows 63% identity with human PCI (hPCI). The putative reactive site is identical to that of hPCI from P5 to P3', suggesting a similar protease specificity. Also the putative heparin binding sites and 'hinge' regions are highly homologous in mouse and hPCI.
Zechmeister-Machhart
M
M
Department of Vascular Biology and Thrombosis Research, University of Vienna, Austria.
Hufnagl
P
P
Uhrin
P
P
Korschineck
I
I
Binder
B R
BR
Geiger
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D018046:Protein C Inhibitor / Q000235:genetics* / Q000378:metabolism*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018046:Protein C Inhibitor