title: An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes.
abstract: An 18.3 kb DNA segment from yeast Saccharomyces cerevisiae VII encompasses the previously characterized MEP1, NUP57 and PPT1 genes as well as seven new open reading frames (ORFs) of at least 100 residues. G6358 is an ubiquitous glutamine-dependent asparagine synthase. G6362 is membrane protein highly homologous to a protein of unknown function in the yeast Schizosaccharomyces pombe. Three ORFs (G6324, G6335 and G6365) have no significant homology with previously reported proteins of characteristic motifs. G6321 and G6359, enclosed in longer ORFs, are not likely to be coding. The segment also contains tRNA genes for Asn, Arg and Ile as well as sigma element and two solo deltas. ORFs and genetic elements are named according to a preliminary working nomenclature.
authors.affiliation: Unité de Biochimie Physiologique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Van Dyck
authors.forename: H
authors.initials: H
authors.lastname: Tettelin
authors.forename: B
authors.initials: B
authors.lastname: Purnelle
authors.forename: A
authors.initials: A
authors.lastname: Goffeau
mesh_terms: D029681:Arabidopsis Proteins; D002352:Carrier Proteins / Q000235:genetics; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D003360:Cosmids; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D008190:Lyases / Q000235:genetics; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D019761:Phosphorus-Oxygen Lyases; D010940:Plant Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D014157:Transcription Factors
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D005656:Fungal Proteins; C071720:MTF1 protein, S cerevisiae; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C074489:RPL9 protein, Arabidopsis; D008190:Lyases; C019895:chorismate synthase; D019761:Phosphorus-Oxygen Lyases