Geroprotector efficiency depends on viability of control population: life span investigation in D. melanogaster.
Ability of the radical spin trap 4-hydroxy-2,2,6,6,-tetramethyl-l-piperidine-N-oxyl (4-hydroxy-TemPO, (4HT)), to delay senescence in D. melanogaster has been studied. The spin trap, at a concentration of 0.2%, was added to the culture medium at the flies development stage. Reliable correlation (r = 0.76, P < 0.05) has been found between the viability of a control population, assessed by the mean life span (LS) value, and the level of LS alteration after geroprotector treatment in a corresponding experimental population. Thereby the local minimum values and local decreases in the mean LS in successive generations of the control line are associated most likely with LS extension effects in the corresponding experimental groups after 4HT treatment, and on the contrary, the local maximum values and local increases in the mean LS of the control line are associated more probably either with LS decreasing effects in the corresponding experimental groups or with the lack of significant differences between the control and experimental groups. A possible mechanism of the discovered phenomenon is discussed, based on the concept about free-radical activity variations. A probability approach to the evaluation of geroprotector efficiency in serial studies is proposed.
Izmaylov
D M
DM
Department of Biological and Chemical Kinetics, N.N. Semenov Institute of Chemical Physics, Russian Academy of Science, Moscow, Russia.
Obukhova
L K
LK
D000375:Aging / Q000187:drug effects*; D000818:Animals; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000502:physiology; D005260:Female; D008136:Longevity / Q000187:drug effects; D008297:Male; D013113:Spin Labels
D003497:Cyclic N-Oxides; D013113:Spin Labels; C001803:tempol