[Characterization of spontaneous liver metastatic model of BALB/c retroperitoneal sarcoma (LMFS) injected in footpad and antitumor effects of HCFU].
A new spontaneous liver metastasis model from LMFS tumor, a retroperitoneal sarcoma of BALB/c mouse origin, was reported. After a subcutaneous injection of LMFS cells in the footpad, amputation of the leg was performed every 5 days from day 5 to day 20, and mice were killed at day 25 under anesthesia. The LMFS cells proliferated at the inoculation site (100% take), and all mice operated after day 15 had metastatic nodules spontaneously in the liver. Local recurrence was not observed in any cases. Liver weight was correlated with tumor growth in the liver. The combination therapy of operation and HCFU was examined in comparison with the footpad injection model. The foot on the injection side was amputated on day 12 with or without HCFU administration, and mice were sacrificed on day 33 under anesthesia. Histological liver metastases were as follows: 1. no treatments: 100%; 2. early phase chemotherapy without operation: 80%; 3. operation without chemotherapy: 80%; 4. postoperative chemotherapy: 20%; 5. preoperative chemotherapy: 40%; and 6. late phase chemotherapy without operation: 100%. These results indicate that operation with chemotherapy is the most useful treatment for liver metastases. Using this liver metastatic model, we are investigating a new combination therapy of operation and anti-cancer drugs for liver metastases.
Fujii
Y
Y
Dept. of Surgery, University of Tokyo.
Itoyanagi
H
H
Hirose
I
I
Hasumi
K
K
Eriguchi
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D003131:Combined Modality Therapy; D005472:Fluorouracil / Q000031:analogs & derivatives / Q000494:pharmacology; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009929:Organ Size; D012186:Retroperitoneal Neoplasms / Q000473:pathology* / Q000601:surgery; D012513:Sarcoma, Experimental / Q000556:secondary* / Q000601:surgery; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; C017367:carmofur; D005472:Fluorouracil