N,N-dimethylsphingosine phosphorylation in human platelets.
Metabolism of sphingosine (Sph) derivatives in human platelets was examined. [3H]Sph was rapidly and heavily phosphorylated into sphingosine 1-phosphate, similarly in resting and stimulated platelets. [14C]N,N-dimethylsphingosine was stable in resting platelets, while it was converted into N,N-dimethylsphingosine 1-phosphate (DMS-1-P), although weakly, in platelets stimulated with thrombin or 12-O-tetradecanoylphorbol 13-acetate. This DMS-1-P formation was inhibited by staurosporine, a potent protein kinase inhibitor. [3H]C2-ceramide was unchanged both in resting and stimulated platelets. Our report is the first to describe production of DMS-1-P in a biological system.
Yatomi
Y
Y
Department of Laboratory Medicine, Yamanashi Medical University, Japan.
Ozaki
Y
Y
Satoh
K
K
Kume
S
S
Ruan
F
F
Igarashi
Y
Y
D001792:Blood Platelets / Q000378:metabolism*; D002518:Ceramides / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D015539:Platelet Activation; D015398:Signal Transduction; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D019311:Staurosporine / Q000494:pharmacology
D002518:Ceramides; C105100:N,N-dimethylsphingosine-1-phosphate; D019311:Staurosporine; C061800:N,N-dimethylsphingosine; D013110:Sphingosine