title: Functional expression on human trophoblasts of interleukin 4 and interleukin 7 receptor complexes with a common gamma chain.
abstract: The gamma chain in the high-affinity receptor complex for interleukin 2(IL-2) is used in receptor complexes for IL-4 and IL-7. We examined expression and function of the gamma chain in trophoblast cells. Flow cytometric studies demonstrated that the human placental cell line and choriocarcinoma cell lines expressed the IL-2R gamma chain. None of these cell lines expressed the IL-2R alpha and beta chains, whereas the alpha chains of IL-4R and IL-7R were present. The gamma chain and IL-4R and IL-7R mRNA were detected in human placental trophoblasts by the in situ hybridization method. The release of human chorionic gonadotropin by trophoblasts was accelerated by IL-4 and IL-7 in a concentration-dependent manner. These findings indicate that IL-4 and IL-7 are involved in trophoblast function.
authors.affiliation: Department of Obstetrics and Gynecology, Nara Medical University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: N
authors.initials: N
authors.lastname: Harada
authors.forename: N
authors.initials: N
authors.lastname: Ishii
authors.forename: T
authors.initials: T
authors.lastname: Morii
authors.forename: S
authors.initials: S
authors.lastname: Sakakura
authors.forename: M
authors.initials: M
authors.lastname: Enomoto
authors.forename: H
authors.initials: H
authors.lastname: Umekage
authors.forename: K
authors.initials: K
authors.lastname: Nishikawa
authors.forename: N
authors.initials: N
authors.lastname: Narita
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Sugamura
authors.forename: H
authors.initials: H
authors.lastname: Morikawa
mesh_terms: D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000378:metabolism; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D007378:Interleukins / Q000494:pharmacology; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018123:Receptors, Interleukin / Q000235:genetics* / Q000378:metabolism; D019948:Receptors, Interleukin-4; D020395:Receptors, Interleukin-7; D014327:Trophoblasts / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D006063:Chorionic Gonadotropin; D007378:Interleukins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D019948:Receptors, Interleukin-4; D020395:Receptors, Interleukin-7