Peculiarities of the cellular ion-transport function of the gastrointestinal tract in hypertensive rats.
1. A study of gastric and intestinal mucosa samples of 15 SHR and 15 normotensive rats of the line WKY was performed by proton-pulsed NMR with a pulsed magnetic field gradient. 2. The diffusion decay shape under the effect of the magnetic field gradient for intestinal mucosa cells in SHR and WKY appeared to be similar and differed drastically for gastric mucosa cells. The signal population of slow protons in SHR was lower and, with regard to fast protons, was higher than that in WKY. 3. The amount of slow protons is connected with low molecular cytoskeleton proteins. The decrease of their amount in hypertensive rats can be used as a test for cell membrane defects and explains, to some extent, the increase of membrane permeability for ions in the case of essential hypertension. This phenomenon is observed only in an acid medium (with increased proton concentration).
Smirnov
Y
Y
Department of Propaedeutics Internal Diseases, Kazan State Medical University, Tatarstan, Russia.
Oslopov
V
V
Sorokina
N
N
Maklakov
A
A
Stolpner
E
E
D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004058:Diffusion; D004064:Digestive System / Q000166:cytology / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011522:Protons; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D003598:Cytoskeletal Proteins; D011522:Protons