Radioprotective efficacy of HT + AET in encapsulated form.
Entrapment of 5-hydroxyl-L-tryptophan (HT) in erythrocyte ghost prepared by hypotonic method and high voltage electric discharge method are nearly same. Release of HT with beta-aminoethylisothiuronium bromide hydrobromide (HT + AET) in in vitro system is rapid but only a portion of the entrapped amount is released. Release of HT + AET in serum marginally increases at 2 hr. Compared to release in in vitro medium the release in serum is less. Survival studies with Swiss albino mice indicates that compared to HT alone, the combination of HT + AET shows about 9 times percentage survival. The same combination in the encapsulated form show comparable percentage survival though the amount needed is 1/200th times compared to free form.
Upadhyay
S N
SN
Radiation Biology Department, Institute of Nuclear Medicine & Allied Sciences, Delhi, India.
Dey
T B
TB
Ghose
A
A
D000818:Animals; D004339:Drug Compounding / Q000379:methods; D004560:Electricity; D004910:Erythrocyte Membrane; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D011817:Rabbits; D011837:Radiation-Protective Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D012701:Serotonin / Q000008:administration & dosage* / Q000097:blood; D000338:beta-Aminoethyl Isothiourea / Q000008:administration & dosage* / Q000493:pharmacokinetics
D011837:Radiation-Protective Agents; D000338:beta-Aminoethyl Isothiourea; D012701:Serotonin