title: A hemoglobin-based blood substitute: transplanting a novel allosteric effect of crocodile Hb.
abstract: Recombinant DNA technology has enabled the large scale production of human hemoglobin in bacteria and yeast. This has opened up a way to produce a hemoglobin-based blood substitute which could replace conventional blood transfusion in some situations. Using our understanding of the structure-function relationships and evolutionary history of hemoglobin it has been possible to improve the oxygen transport properties of the molecule and solve a number of problems associated with the use of natural hemoglobin as a cell-free blood substitute.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, U.K.
authors.forename: N
authors.initials: N
authors.lastname: Komiyama
authors.forename: J
authors.initials: J
authors.lastname: Tame
authors.forename: K
authors.initials: K
authors.lastname: Nagai
mesh_terms: D000489:Alligators and Crocodiles / Q000097:blood*; D000818:Animals; D001802:Blood Substitutes / Q000378:metabolism*; D006454:Hemoglobins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D011994:Recombinant Proteins