Sodium-dependent adenosine transport is diminished in brush border membrane vesicles from hypothyroid rat kidney.
Studies of the uptake of [3H]adenosine ([3H]ADO) were performed using brush border membrane vesicles (BBMV) from normal (N) and hypothyroid (Tx) rat kidneys, to test if decreased Na+ reabsorption in hypothyroidism might be associated with abnormalities in ADO transport. [3H]ADO uptake (1-10 micromol) for both conditions was measured in the presence of Na+ (10-150 mmol/l); the effects of dipyridamole (10 micromol/l) and 1, 3-dipropyl-8-(2-amino-4-chlorophenyl)xanthine (PACPX, 10 micromol/l) were also studied. Na+-stimulated ADO uptake was decreased in Tx BBMV. Michaelis-Menten constants showed a decreased ADO carrier affinity (Km 2.46 +/- 0.14 in N, vs Km 4.46 +/- 0.88 micromol/l in Tx, P<0.05), with no change in the number of carriers (Vmax 295 +/- 25 in N, vs 229.2 +/- 56 pmol.min-1.mg protein in Tx). Na+ affinity remained unchanged (K Na+ 11.5 +/- 3.5 in N, vs K Na+ 12.72 +/- 0.7 mmol/l in Tx). Inhibition of Na+-dependent ADO transport was 50% in N as opposed to 58% in Tx with dipyridamole, and 72% in N versus 47% in Tx with PACPX. These results suggest that decreased Na+-dependent ADO cotransport contributes to the diminished tubular reabsorption that occurs in hypothyroidism.
Martínez
F
F
Department of Pharmacology, Facultad de Medicina de la UASLP, Av. Venustiano Carranza No. 2405, 78210-Los Filtros, San Luis Potosí, Mexico.
Franco
M
M
Quintana
A
A
Herrera-Acosta
J
J
D000241:Adenosine / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007037:Hypothyroidism / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000494:pharmacology*
D012964:Sodium; D000241:Adenosine