Efficient transduction of green fluorescent protein in spinal cord neurons using adeno-associated virus vectors containing cell type-specific promoters.
In this study, we have evaluated the capacity of recombinant adeno-associated virus (rAAV) vectors, containing cell type-specific promoters, to transduce neurons in vivo in the normal adult rat spinal cord. The neuron-specific enolase (NSE) promoter and the platelet-derived growth factor (PDGF) B-chain promoter were used to direct expression of a 'humanized' form of the gene for green fluorescent protein (GFP). Neuron-specific rAAVs were injected into the mid-cervical regions of adult rat spinal cords. At 10-14 days, expression was detected in all animals and persisted for up to 15 weeks. Immunocytochemical and morphological profiles of transduced cells were consistently neuronal, and there was no evidence of transgene expression in glial elements. Transduction efficiencies for the NSE and PDGF rAAVs were estimated at 15 and 45 infectious particles per GFP-positive neuron, respectively, in the absence of detectable adenovirus. This study strongly supports a role for rAAV vectors in CNS gene therapy and lays the groundwork for delivery of more functional genes to spinal cord neurons as a possible way to enhance spinal cord repair following injury.
Peel
A L
AL
Department of Neuroscience, University of Florida Brain Institute, Guinesville 32607, USA.
Zolotukhin
S
S
Schrimsher
G W
GW
Muzyczka
N
N
Reier
P J
PJ
D000818:Animals; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D008856:Microscopy, Fluorescence; D009474:Neurons; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats; D013116:Spinal Cord; D013997:Time Factors; D019076:Transgenes
D008164:Luminescent Proteins; D010982:Platelet-Derived Growth Factor; D049452:Green Fluorescent Proteins; D010751:Phosphopyruvate Hydratase