title: [Functional screening of p53 status in tumor cells using Saccharomyces cerevisiae].
abstract: We have detected both germ-line and somatic p53 mutations in lymphocytes, cell lines and tumor tissues using a functional analysis of p53 tumor suppressor gene based on yeast transcription assay. Through our screening projects of the p53 gene, a number of missense p53 mutations were identified as loss-of-function mutations. This method, previously termed FASAY, is rapid, sensitive, less-expensive and can be automated for screening both somatic and germ-line p53 mutations.
authors.affiliation: Dept. of Clinical Oncology, Tohoku University.
authors.forename: L Q
authors.initials: LQ
authors.lastname: Jia
authors.forename: C
authors.initials: C
authors.lastname: Ishioka
authors.forename: M
authors.initials: M
authors.lastname: Osada
authors.forename: M
authors.initials: M
authors.lastname: Gamo
authors.forename: H
authors.initials: H
authors.lastname: Shimodaira
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: R
authors.initials: R
authors.lastname: Kanamaru
mesh_terms: D016158:Genes, p53 / Q000235:genetics / Q000502:physiology; D018095:Germ-Line Mutation; D006801:Humans; D008214:Lymphocytes; D009154:Mutation; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured