The beta-phaseolin 5' matrix attachment region acts as an enhancer facilitator.
MARs found flanking the beta-phaseolin gene (phas) were tested for insulating activity in an enhancer blocking assay. True insulators should block enhancer dependent expression of a reporter gene when placed between the enhancer and a promoter. Insertion of phas 3' MAR or coding sequences lowered CaMV 35S enhancer driven GUS expression from the phas basal promoter, indicating a distance dependence of the 35S enhancer. 5' MAR or 5' MAR core fragments could not act as independent enhancers when fused to the phas basal promoter, and did not lower expression when inserted in the enhancer blocking assay construct, indicating that they facilitated 35S enhancer expression at a distance when located between the enhancer and the promoter.
van der Geest
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AH
Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, College Station 77843-3155, USA.
Hall
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TC
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D007887:Fabaceae / Q000235:genetics; D017343:Genes, Plant; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal
D004268:DNA-Binding Proteins; D010940:Plant Proteins; C048826:phaseolin protein, Phaseolus vulgaris