The cellular localization of vasoactive intestinal peptide (VIP) in the mouse median eminence by immuno-electron microscopy.
The aim of this study was to examine, by use of pre-embedding immunocytochemistry, the ultrastructural localization of vasoactive intestinal peptide (VIP) immunoreactivity in the mouse median eminence. VIP immunoreactivity was observed in axonal profiles. The VIP-immunoreactive axonal profiles were in close proximity to non-immunoreactive axonal profiles that contained dense granular vesicles and clear vesicles and also to processes of tanycytes. VIP-immunoreactive terminals were observed in the proximity of the perivascular space and in the neuropil. Our results suggest that VIP-immunoreactive axon terminals may possibly interact with other non-immunoreactive axon terminals containing peptide and/or other transmitters at the level of the median eminence or may be released to the portal vasculature thereby to effect anterior pituitary cells.
Dalcik
H
H
Department of Hisotology and Embryology, Gulhane Military Medical Academy, Ankara, Turkey.
Chen
I L
IL
Dalcik
C
C
Phelps
C J
CJ
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D008473:Median Eminence / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D051379:Mice; D016253:Microscopy, Immunoelectron; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D016707:Tissue Fixation; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D014660:Vasoactive Intestinal Peptide