Birth of a western lowland gorilla (Gorilla gorilla gorilla) following in vitro fertilization and embryo transfer.
A 21-year-old multiparous female exhibiting 31-41 day menstrual cycles was given hFSH (225 IU/day, Metrodin 75, from cycle day 3 through 9 (menses = day 1) and hCG (10,000 IU, Profasi, on day 10 to stimulate follicular development. At 35 h after hCG, under isoflurane (AErrane) anesthesia, follicles were aspirated by controlled suction under transvaginal ultrasound guidance. Metaphase II oocytes (n = 11) were placed in modified human tubal fluid (mHTF, 100 microliters) medium under oil at 37 degrees C in humidified 5% CO2. Frozen semen, collected by voluntary ejaculation, was thawed (70 degrees C H2O bath, 6 sec), diluted slowly, centrifuged, and resuspended in mHTF, and 160,000 motile spermatozoa/ml were added at 6 h after oocyte recovery. At 21 h postinsemination (p.i.) eight oocytes were at the two-cell stage, five were cryopreserved, and three were cultured to the six- to eight-cell stage in mHTF with granulosa cells before transcervical uterine transfer at 47 h p.i. using a Teflon catheter. Micronized progesterone (400 mg/d) was orally administered for 10 weeks posttransfer (p.t.). Ultrasound examination revealed a single fetus at 15 weeks p.t., and unassisted delivery of a live 1.37 kg female infant occurred at 29 weeks. Am. J. Primatol. 41:247-260, 1997.
Pope
C E
CE
Cincinnati Zoo and Botanical Garden, Center for Reproduction of Endangered Wildlife, Ohio, USA.
Dresser
B L
BL
Chin
N W
NW
Liu
J H
JH
Loskutoff
N M
NM
Behnke
E J
EJ
Brown
C
C
McRae
M A
MA
Sinoway
C E
CE
Campbell
M K
MK
Cameron
K N
KN
Owens
O M
OM
Johnson
C A
CA
Evans
R R
RR
Cedars
M I
MI
D000818:Animals; D015925:Cryopreservation / Q000662:veterinary; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D006071:Gorilla gorilla; D008597:Menstrual Cycle / Q000502:physiology; D009865:Oocytes; D012662:Semen Preservation / Q000662:veterinary
D005640:Follicle Stimulating Hormone