Effects of chelation therapy on hepatic glutathione, lipid peroxidation and phospholipid contents in lead-poisoned rats.
Hepatic lipid peroxidation, glutathione and phospholipid contents of homogenate prepared from the liver of lead-intoxicated male rats treated with 0.3 m mol/kg CaNa2EDTA and DMSA for 8 weeks, either alone or in combination, were investigated. A significant increase in hepatic malondialdehyde (MDA) and a reduction in glutathione levels was noticed. While a marginal decrease in phosphatidyl choline (PC) level was noticed, no effect on phospholipid contents was seen. Treatment with all the three chelating agents elicited decrease in PC level. DMSA alone was partially effective in restoring lead-induced altered hepatic glutathione and MDA levels. Combined treatment may have an adverse effects on hepatic tissue and does not seem to produce immediate recoveries in the lead-induced hepatic damage.
Sachan
A S
AS
Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior.
Kannan
G M
GM
Kumar
P
P
Flora
S J
SJ
D000818:Animals; D002614:Chelating Agents / Q000627:therapeutic use; D015913:Chelation Therapy; D004492:Edetic Acid / Q000627:therapeutic use; D005978:Glutathione / Q000378:metabolism*; D007855:Lead Poisoning / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D015227:Lipid Peroxidation / Q000502:physiology*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D004113:Succimer / Q000627:therapeutic use
D002614:Chelating Agents; D010743:Phospholipids; D008315:Malondialdehyde; D004492:Edetic Acid; D004113:Succimer; D005978:Glutathione