Method of site-directed mutagenesis using long primer-unique site elimination and exonuclease III.
Nicolas
G
G
INSERM U409, Faculté de Médecine Bichat, Paris, France. gnicolas@infobiogen.fr
Pedroni
S
S
Fournier
C
C
Gautero
H
H
Lecomte
M C
MC
D017931:DNA Primers; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic / Q000235:genetics
D017931:DNA Primers; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D015245:Deoxyribonuclease BamHI