Cysteine biosynthesis in higher plants: a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph.
A cDNA clone, Sat-52, encoding a novel isoform of serine acetyltransferase (EC 2.3.1.30) was isolated by functional complementation of an Escherichia coli cysE mutant defective in serine acetyltransferase. The 1158 base pair clone contains a full-length open reading frame encoding a deduced protein of 312 amino acids with an M(r) of 32.77 kDa. Northern analysis revealed a single transcript of ca 1.19 kb that did not increase in abundance under sulfate limitation. Genomic Southern hybridization suggests the presence of a single copy of the Sat-52 gene.
Howarth
J R
JR
Plant Sciences Laboratory, University of St. Andrews, UK.
Roberts
M A
MA
Wray
J L
JL
D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D003545:Cysteine / Q000096:biosynthesis*; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D017343:Genes, Plant; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D009154:Mutation; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D051050:Serine O-Acetyltransferase
D018076:DNA, Complementary; D018744:DNA, Plant; D000123:Acetyltransferases; D051050:Serine O-Acetyltransferase; D003545:Cysteine