The uptake of Mn-DPDP by hepatocytes is not mediated by the facilitated transport of pyridoxine.
Manganese-dipyridoxal diphosphate (Mn-DPDP) is a liver-selective contrast agent selectively taken up by the hepatocytes. Because of the analogy of structure with pyridoxine (vitamin B6), it was previously suggested that this compound can be selectively taken up by the facilitated transport of vitamers B6. To understand the uptake mechanism, an in vivo binding study was performed based on a competition between 54Mn-DPDP and pyridoxine on the one hand, and Mn-DPDP and [3H]pyridoxine on the other. We found that the [3H]pyridoxine levels in the liver were not significantly different 5 min after intravenous administration of several doses of Mn-DPDP (5 nmol/kg to 50 mumol/kg): 5.0 +/- 0.3% of the injected dose/g tissue. The content of 54Mn (administered as 54Mn-DPDP) in the liver was not affected by a saturation dose of pyridoxine (1 mmol/kg) and was found to be constant (+/- 10% of the injected dose/g tissue) for 60 min. These experiments showed that the uptake of Mn-DPDP is not mediated by the transporter of pyridoxine.
Gallez
B
B
Laboratory of Medicinal Chemistry, University of Louvain, Brussels, Belgium.
Baudelet
C
C
Adline
J
J
Charbon
V
V
Lambert
D M
DM
D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport; D003287:Contrast Media / Q000737:chemistry / Q000493:pharmacokinetics*; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008345:Manganese; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D011736:Pyridoxine / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*
D003287:Contrast Media; D008345:Manganese; D011732:Pyridoxal Phosphate; D004492:Edetic Acid; D011736:Pyridoxine; C060076:N,N'-bis(pyridoxal-5-phosphate)ethylenediamine-N,N'-diacetic acid