title: Expression and retinoid modulation of N-arginine dibasic convertase and an aminopeptidase-B in human neuroblastoma cell lines.
abstract: Under retinoic acid exposure, the three SK-N-BE(2)-derived human neuroblastoma cell lines, BE(2)-NA, BE(2)-SA and BE(2)-M17 undergo mainly differentiation, apoptosis or continue to proliferate, respectively. We have used this model system to study the modulation of the transcriptional expression of putative processing enzymes, two novel metallopeptidases; i.e. N-arginine dibasic convertase (NRD convertase; EC 3.4,24,61) and an aminopeptidase-B after exposure of the cells either to retinoic acid or to synthetic retinoid analogs. The data indicate that the two respective enzymes are differently modulated in the various cell lines. Whereas aminopeptidase-B expression is enhanced in most cases, NRD convertase appears to undergo opposite regulation in proliferating versus differentiating neuroblastoma cells. It is concluded that both genes might contain retinoic acid regulatory elements (RARE) in their promoters.
authors.affiliation: Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, URA CNRS 1682, Université Pierre et Marie Curie, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Draoui
authors.forename: L
authors.initials: L
authors.lastname: Bellincampi
authors.forename: V
authors.initials: V
authors.lastname: Hospital
authors.forename: S
authors.initials: S
authors.lastname: Cadel
authors.forename: T
authors.initials: T
authors.lastname: Foulon
authors.forename: A
authors.initials: A
authors.lastname: Prat
authors.forename: N
authors.initials: N
authors.lastname: Barré
authors.forename: U
authors.initials: U
authors.lastname: Reichert
authors.forename: G
authors.initials: G
authors.lastname: Melino
authors.forename: P
authors.initials: P
authors.lastname: Cohen
mesh_terms: D000626:Aminopeptidases / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D019281:Dimerization; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism; D009447:Neuroblastoma; D012333:RNA, Messenger / Q000032:analysis; D012176:Retinoids / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D012176:Retinoids; D000626:Aminopeptidases; C019721:aminopeptidase B; D008666:Metalloendopeptidases; C087976:nardilysin