title: Binding of a soluble p70 killer cell inhibitory receptor to HLA-B*5101: requirement for all three p70 immunoglobulin domains.
abstract: Lysis of target cells by natural killer (NK) cells can be prevented by killer cell inhibitory receptors (KIR) specific for major histocompatibility complex class I molecules. Functional studies have identified two distinct p58 KIR, each reactive with a different group of HLA-C allotypes, and distinct p70 KIR specific for some HLA-B or HLA-A allotypes. The NK specificities for each group of HLA-C allotypes have been reproduced by direct binding of recombinant soluble p58 molecules. Here, we show that a soluble p70 KIR binds to HLA-B*5101, but not to HLA-A or HLA-C molecules. Truncated soluble forms of the HLA-B*5101-specific p70 KIR, including one with two immunoglobulin (Ig) domains reactive with a monoclonal antibody that blocks p70 KIR function, did not bind to HLA-B*5101, indicating that all three Ig domains are required for binding.
authors.affiliation: Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, Rockville, MD 20852-1727, USA.
authors.forename: S
authors.initials: S
authors.lastname: Rojo
authors.forename: N
authors.initials: N
authors.lastname: Wagtmann
authors.forename: E O
authors.initials: EO
authors.lastname: Long
mesh_terms: D015235:HLA-B Antigens / Q000378:metabolism*; D059949:HLA-B51 Antigen; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry*; D007694:Killer Cells, Natural / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology*; D011485:Protein Binding / Q000276:immunology; D011971:Receptors, Immunologic / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology
chemical_list: D015235:HLA-B Antigens; D059949:HLA-B51 Antigen; D007136:Immunoglobulins; D010446:Peptide Fragments; D011971:Receptors, Immunologic; C098698:p70 natural killer cell receptor