Presence of argininosuccinate synthetase in glial cells as revealed by peptide-specific antisera.
The presence and the possibility of induction of argininosuccinate synthetase in a glial cell line were investigated. For this purpose, antisera were produced against peptides representing partial sequences 196-222 and 337-349, respectively, of the mouse liver enzyme. Both antisera were shown to be monospecific for argininosuccinate synthetase. In Western blot experiments, immunoreactivity was found in mouse liver and brain homogenates. Only weak immunoreactivity was detectable in homogenates of cultured glioma cells, C6-BU-1. However, when the glioma cells were treated with either bacterial lipopolysaccharide, interferon-gamma, or a combination of both, argininosuccinate synthetase immunoreactivity was increased. The findings demonstrate that this enzyme is present in glial cells and is induced under conditions which stimulate persistent production of nitric oxide. The antisera will be a valuable tool for further investigations on arginine synthesis in brain as well as peripheral cells.
Schmidlin
A
A
Physiologisch-chemisches Institut der Universität, Tübingen, Germany.
Kalbacher
H
H
Wiesinger
H
H
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001124:Argininosuccinate Synthase / Q000032:analysis*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009457:Neuroglia / Q000166:cytology / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism; D010455:Peptides / Q000276:immunology; D011817:Rabbits; D014407:Tumor Cells, Cultured
D000906:Antibodies; D010455:Peptides; D009569:Nitric Oxide; D001124:Argininosuccinate Synthase