title: Overexpression of nestin and vimentin in the ependyma of spinal cords from hydrocephalic infants.
abstract: The ependyma of the spinal central canal in cases of hydrocephalus shows abnormalities which vary with the aetiology of ventricular dilatation. To determine whether these ependymal changes are developmental or reactive in nature, immunohistochemical findings were compared between nine normal controls and 12 cases of hydrocephalus (three each of congenital aqueductal stenosis. Dandy-Walker malformation, Chiari type II malformation, and post-haemorrhagic hydrocephalus) using antisera to nestin, vimentin and glial fibrillary acidic protein. The main pathological findings were disruption of ependymal layer, apparent pseudostratification of ependyma, expansion, cleft or syrinx formation in relation to the central canal, and ependymal rosette formation. In normal developing fetal spinal cord, nestin and vimentin were expressed mainly in pseudostratified ependymal cells and radial fibres in the median septum. In cases with congenital hydrocephalus (congenital aqueductal stenosis. Dandy-Walker malformation, and Chiari type II malformation), nestin was overexpressed in immature ependymal cells, and strong vimentin immunoreactivity was detected in the long tract of radial fibres in the median septum. Nestin and vimentin were also expressed in small cells and their fibres which covered areas denuded of ependymal cells in cases of Chiari type II malformation and post-haemorrhagic hydrocephalus. Two conclusions are suggested by this report. First, the ependyma of the spinal central canal in congenital hydrocephalus shows a delay in maturation of radial glial cells into mature astrocytes and ependymal cells. Second, areas of ependymal denudation may be repaired by the immature glial cells derived from subependymal cells.
authors.affiliation: Department of Pathology, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Takano
authors.forename: L E
authors.initials: LE
authors.lastname: Becker
mesh_terms: D004805:Ependyma / Q000378:metabolism*; D005260:Female; D006801:Humans; D006849:Hydrocephalus / Q000378:metabolism*; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins; D064231:Nestin; D013116:Spinal Cord / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*
chemical_list: D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D014746:Vimentin