The cDNA cloning and transient expression of a chicken gene encoding cytochrome P-450scc.
A cDNA clone, pcSCC1, isolated from a chicken adrenal gland library showed about 62% nucleotide sequence similarity to those of mammalian and rainbow trout P-450scc cDNAs and was proved to be the clone for chicken P-450scc by the enzymatic activity of converting 25-hydroxycholesterol to pregnenolone demonstrated in the transfected COS-7 cells. Comparison of deduced amino acid sequences shows significant conservation of heme- and steroid-binding domains but little similarity in the putative N-terminal presequence region among chicken, trout and mammals. The chicken cDNA lacks a polyadenylation signal in its 3'-UTR. Sequencing of a 3' end region of the P-450scc gene (CYP11A1) suggests that a region containing the polyadenylation signal is spliced out from the pre-mRNA.
Nomura
O
O
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Nakabayashi
O
O
Nishimori
K
K
Mizuno
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells / Q000378:metabolism; D002645:Chickens / Q000235:genetics*; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D008297:Male; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics; D010053:Ovary / Q000378:metabolism; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution; D014162:Transfection
D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; D002786:Cholesterol Side-Chain Cleavage Enzyme