Aromatic DNA adducts in foundry workers in relation to exposure, life style and CYP1A1 and glutathione transferase M1 genotype.
Levels of aromatic DNA adducts in foundry workers and controls were followed at four annual samplings. During this time exposure to polycyclic aromatic hydrocarbons (PAH) decreased and the level of DNA adducts decreased accordingly. In the total group exposure was related to the level of adducts. Adduct levels correlated with urinary 1-hydroxypyrene (LOGU1OH), air benzo[a]pyrene, weekly working hours and daily cigarette consumption. In a multivariate model 1-hydroxypyrene had a consistent effect. Neither glutathione transferase M1 (GSTM1) nor cytochrome P450 1A1 (CYP1A1) genotypes had clear effects. Yet the individuals lacking GSTM1 had a stronger effect of LOGU1OH and some effect by other sources of PAH, such as charcoal broiled food, although all these variables were not significant in the multivariate model. The rare individuals with a CYP1A1 polymorphism MspI containing an amino acid change at isoleucine had an increased level of adducts. The results showed that the postlabelling method used was able to detect an increase in aromatic DNA adducts in leukocytes when exposure to benzo[a]pyrene in air was approximately 5 ng/m3. At such low levels smoking and charcoal broiled food may be important contributors to adducts.
Hemminki
K
K
Department of Biosciences, Karolinska Institute, Novum, Huddinge, Sweden.
Dickey
C
C
Karlsson
S
S
Bell
D
D
Hsu
Y
Y
Tsai
W Y
WY
Mooney
L A
LA
Savela
K
K
Perera
F P
FP
D000328:Adult; D000704:Analysis of Variance; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D018736:DNA Adducts / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008019:Life Style; D008297:Male; D016273:Occupational Exposure; D011083:Polycyclic Compounds / Q000097:blood*; D012044:Regression Analysis
D018736:DNA Adducts; D011083:Polycyclic Compounds; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase