Use of polymerase chain reaction in analysing recombinant cDNA clones encoding storage proteins of chickpea Cicer arietinum L.
A simple and reliable method was undertaken for the use of polymerase chain reaction in analyzing cDNA clones. Amplification was done of the inserts from positive legumin clones isolated from a cDNA library constructed from developing chickpea cotyledons in the expression vector, gt11. Amplification was made simple by using oligonucleotide primers which allowed convenient sizing, subcloning and sequencing of inserts by di-deoxy chain termination method. This simple method may provide opportunity to isolate large number of agronomically important genes from gene libraries.
Saha
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National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India.
Kansal
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Koundal
K R
KR
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D000097793:Legumins
D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D000097793:Legumins