title: Synthetic study of phosphopeptides related to heat shock protein HSP27.
abstract: Two kinds of phosphoserine-containing peptides related to HSP27 were synthesized by the Boc- or Fmoc-mode solid-phase method based on prephosphorylation strategy. In the case of the Boc strategy, the O-phosphono group of the phosphoserine residue was protected with the cyclopentyl or cyclohexyl group. On the other hand, N'-Fmoc-O-[(benzyloxy)-hydroxyphosphinyl]serine was employed in case of the Fmoc strategy. Consequently, it has become-feasible to utilize conventional solid-phase methods for synthesizing any phosphopeptides which are required to elucidate biochemical significance of protein phosphorylation.
authors.affiliation: Department of Chemistry, Faculty of Science and Technology, Kinki University, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Wakamiya
authors.forename: R
authors.initials: R
authors.lastname: Togashi
authors.forename: T
authors.initials: T
authors.lastname: Nishida
authors.forename: K
authors.initials: K
authors.lastname: Saruta
authors.forename: J
authors.initials: J
authors.lastname: Yasuoka
authors.forename: S
authors.initials: S
authors.lastname: Kusumoto
authors.forename: S
authors.initials: S
authors.lastname: Aimoto
authors.forename: K Y
authors.initials: KY
authors.lastname: Kumagaye
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Nagata
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006360:Heat-Shock Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010748:Phosphopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D006360:Heat-Shock Proteins; D010748:Phosphopeptides