Hydrophobic chromatography of the HL-60 cellular fraction co-binding with hexamethylene bisacetamide.
Methods of separating N-acetyl-1,6-diaminohexane (NADAH) and its immobilization to diol-silica have been developed. Hexamethylene bisacetamide (HMBA) and its metabolite NADAH are used as inducers of leukemia cell differentiation. The inducing mechanism of HMBA is still not clear. Experiments show that HMBA and NADAH undergo relatively strong hydrophobic reactions and do not readily undergo ion-exchange with the proteins of the cytosolic fraction of HL-60 cells during immobilization of NADAH; the retention time of the proteins was longer than that of the phosphatides. These results show that the adsorption of HMBA and NADAH to proteins was higher than that to phosphatides. The expected biospecific receptor binding with HMBA has not been found.
Xie
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D
Administration Department of Lanzhou General Hospital of Lanzhou Command PLA, China.
Li
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S
Ren
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L
D000081:Acetamides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism
D000081:Acetamides; D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; C050895:N-acetyl-1,6-diaminohexane; C014026:hexamethylene bisacetamide