title: Osteoclast precursors in bone marrow and peritoneal cavity.
abstract: Osteoclasts differentiate from cells that share some phenotypes with mature macrophages and monocytes, but early precursors for osteoclasts still remain obscure. To characterize osteoclast precursors, using monoclonal anti-c-Fms and anti-c-Kit antibodies, bone marrow cells were separated and the frequency of clonogenic progenitors were measured. Osteoclast precursors in the bone marrow mainly expressed c-Kit and diminished in frequency when they expressed c-Fms. In contrast to bone marrow, the precursors in the peritoneal cavity were enriched with a population of c-Fms+. Injection of these antibodies into mice demonstrated that peritoneal osteoclast precursors were sensitive to anti-c-Fms but not to anti-c-Kit antibodies, whereas those in bone marrow only declined in the presence of both antibodies. Meanwhile, c-Fms as opposed to c-Kit played an essential role in the generation of osteoclasts in cultures. We also compared osteoclast precursors with colony forming cells (CFU-M) by a macrophage colony stimulating factor. CFU-M in bone marrow decreased when anti-c-Kit antibody was administered and no CFU-M was detected in peritoneum. In this study, we show differences between proliferative potential osteoclast precursors maintained in bone marrow and peritoneum and between CFU-M and osteoclast precursors.
authors.affiliation: Department of Immunology, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: A
authors.initials: A
authors.lastname: Miyamoto
authors.forename: T
authors.initials: T
authors.lastname: Yamane
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
authors.forename: S
authors.initials: S
authors.lastname: Sugawara
authors.forename: S
authors.initials: S
authors.lastname: Nishikawa
authors.forename: S
authors.initials: S
authors.lastname: Nishikawa
authors.forename: T
authors.initials: T
authors.lastname: Sudo
authors.forename: H
authors.initials: H
authors.lastname: Yamazaki
authors.forename: T
authors.initials: T
authors.lastname: Kunisada
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015415:Biomarkers; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured / Q000737:chemistry / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010010:Osteoclasts / Q000737:chemistry / Q000166:cytology*; D010529:Peritoneal Cavity / Q000166:cytology*; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis / Q000378:metabolism; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*
chemical_list: D015415:Biomarkers; D019009:Proto-Oncogene Proteins c-kit