title: Role of V-ATPase-rich cells in acidification of the male reproductive tract.
abstract: Specialized proton-secreting cells play important physiological roles in a variety of tissues. On the basis of the immunocytochemical detection of carbonic anhydrase and V-ATPase in distinct epithelial cells of the epididymis and vas deferens, we predicted that the vacuolar V-ATPase that is located on the apical membrane of these cells should be a major contributor to luminal acidification in parts of the male reproductive tract. Physiological studies using the proton-selective vibrating probe in the vas deferens confirmed this hypothesis. As discussed recently, maintenance of the pH of the reproductive tract is probably under tight physiological control, by analogy with the situation in the kidney. Manipulation of luminal pH might, therefore, provide a point of intervention for the regulation of male fertility. In addition, it is possible that some cases of unexplained male infertility might result from defective acidification, resulting either from pathological states or potentially from environmental factors that may inhibit proton secretory pathways.
authors.affiliation: Renal Unit, Massachusetts General Hospital, Boston, USA. brown@receptor.mgh.harvard.edu
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: P J
authors.initials: PJ
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Breton
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004822:Epididymis / Q000201:enzymology / Q000502:physiology; D005298:Fertility; D005837:Genitalia, Male / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000201:enzymology; D007668:Kidney / Q000502:physiology; D018942:Macrolides; D008297:Male; D008870:Microtubules / Q000502:physiology; D009928:Organ Specificity; D054328:Proton Pump Inhibitors; D017494:Proton Pumps / Q000502:physiology*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats; D025262:Vacuolar Proton-Translocating ATPases; D014649:Vas Deferens / Q000201:enzymology / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D018942:Macrolides; D054328:Proton Pump Inhibitors; D017494:Proton Pumps; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D002256:Carbonic Anhydrases