title: Amyloid beta peptides stimulate tissue-type plasminogen activator but not recombinant prourokinase.
abstract: Tissue-type plasminogen activator (rt-PA) and prourokinase (rscu-PA) have been tested with respect to the influence of amyloid beta peptides on plasminogen activation which was monitored by cleavage of the chromogenic plasmin substrate S-2251. It was shown that rt-PA is stimulated by amyloid beta peptides at concentrations of 10 micrograms/ml in contrast to prourokinase, which does not alter its catalytic properties in presence of amyloid beta peptides. The stimulation of rt-PA can be inhibited by tranexamic acid indicating a molecular mode of stimulation similar to the fibrin mediated stimulation of rt-PA.
authors.affiliation: Gruenenthal GmbH, Department of Molecular Pharmacology, Aachen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wnendt
authors.forename: I
authors.initials: I
authors.lastname: Wetzels
authors.forename: W A
authors.initials: WA
authors.lastname: Günzler
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000933:Antifibrinolytic Agents / Q000494:pharmacology; D002863:Chromogenic Compounds; D003864:Depression, Chemical; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D011994:Recombinant Proteins / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014148:Tranexamic Acid / Q000494:pharmacology; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D000933:Antifibrinolytic Agents; D002863:Chromogenic Compounds; D011994:Recombinant Proteins; D014148:Tranexamic Acid; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator