title: CRF2-4: isolation of cDNA clones encoding the human and mouse proteins.
abstract: To search for genes which encode human interferon receptor molecules, we applied the technique of exon trapping to human chromosome 21 yeast artificial chromosomes (YACs). A full length cDNA encoding human CRF2-4 was identified which is shown here to be widely expressed in human tissues. The human CRF2-4 cDNA was used as a probe to screen a mouse macrophage cDNA library. A mouse CRF2-4 cDNA was isolated which encodes a 349-amino-acid (aa) polypeptide that is 69% identical at the aa level with its human counterpart. Mouse CRF2-4 is predicted to be another member of the interferon receptor-class II cytokine receptor family.
authors.affiliation: Department of Surgery, San Francisco VA Medical Center, CA 94131, USA. gibbs.verna@sanfrancisco.va.gov
authors.forename: V C
authors.initials: VC
authors.lastname: Gibbs
authors.forename: D
authors.initials: D
authors.lastname: Pennica
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002891:Chromosomes, Human, Pair 21; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D005091:Exons; D005821:Genetic Techniques; D006801:Humans; D053710:Interleukin-10 Receptor beta Subunit; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D018121:Receptors, Cytokine / Q000235:genetics* / Q000378:metabolism*; D017471:Receptors, Interferon / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; C081846:IL10RB protein, human; D053710:Interleukin-10 Receptor beta Subunit; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D017471:Receptors, Interferon