The proteoglycan decorin links low density lipoproteins with collagen type I.
Decorin is a small dermatan sulfate-rich proteoglycan which binds to collagen type I in vitro and in vivo. In atherosclerotic lesions the contents of low density lipoprotein (LDL), decorin, and collagen type I are increased, and ultrastructural studies have suggested an association between LDL and collagen in the lesions. To study interactions between LDL, decorin, and collagen type I, we used solid phase systems in which LDL was coupled to a Sepharose column, or in which LDL, decorin, or collagen type I was attached to microtiter wells. The interaction between LDL and decorin in the fluid phase was evaluated using a gel mobility shift assay. We found that LDL binds to decorin by ionic interactions. After treatment with chondroitinase ABC, decorin did not bind to LDL, showing that the glycosaminoglycan side chain of decorin is essential for LDL binding. Acetylated and cyclohexanedione-treated LDL did not bind to decorin, demonstrating that both lysine and arginine residues of apoB-100 are necessary for the interaction. When collagen type I was attached to the microtiter plates, only insignificant amounts of LDL bound to the collagen. However, if decorin was first allowed to bind to the collagen, binding of LDL to the decorin-collagen complexes was over 10-fold higher than to collagen alone. Thus, decorin can link LDL with collagen type I in vitro, which suggests a novel mechanism for retention of LDL in collagen-rich areas of atherosclerotic lesions.
Pentikäinen
M O
MO
Wihuri Research Institute, Kalliolinnantie 4, SF-00140 Helsinki, Finland.
Oörni
K
K
Lassila
R
R
Kovanen
P T
PT
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D058575:Decorin; D016326:Extracellular Matrix Proteins; D008077:Lipoproteins, LDL / Q000378:metabolism*; D011485:Protein Binding; D011509:Proteoglycans / Q000378:metabolism*
D058575:Decorin; D016326:Extracellular Matrix Proteins; D008077:Lipoproteins, LDL; D011509:Proteoglycans; D003094:Collagen