title: [A study on complicated chromosome translocation by fluorescence in situ hybridization (FISH) of G-banded chromosomes].
abstract: An experimental method of fluorescence in situ hybridization (FISH) with conventionally G-band chromosomes was developed to analyze the complex chromosome rearrangements of patients. In order to obtain optimal results of both G-banding and fluorescence signals, it was essential that G-banded chromosomes were fixed with formaldehyde prior to fluorescence in situ hybridization. The karyotype of a patient with spontaneous abortion might be described as 46, XX, t(1; 5; 12) (1pter-->1q25 : : 12q24-->12qter; 5qter-->5p11 : : 1q25-->1qter; 12pter-->12q24 : : 5p11-->5pter) by the analysis of conventional cytogenetics. However, her karyotype should be identified as 46, XX, t(1; 5; 12) (1pter-->1q23 : : 12q22-->12qter; 5qter-->5p11 : : 1q25-->1qter; 12pter-->12q22 : : 1q23-->1q25 : : 5p11-->5pter) with this newly established method. This investigation indicates that this technique described above is a powerful tool to detect complex chromosome rearrangements of patients.
authors.affiliation: Nanjing Normal University.
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000328:Adult; D002871:Chromosome Banding; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D014178:Translocation, Genetic