Structures and activity of cellulase inhibitors enzymatically synthesized from cellooligosaccharides and 1-deoxynojirimycin.
Cellulase inhibitors were synthesized from cellooligosaccharides and 1-deoxynojirimycin (DNJ) by transglycosylation, using a commercial cellulase. The structures of these cellulase inhibitors were proved to be 4-O-beta-cellobiosyl-DNJ, 4-O-beta-D-glucopyranosyl-DNJ and 6-O-beta-cellobiosyl-DNJ by the results of 13C- and 1H-NMR analyses. The inhibitory activity of each inhibitor was investigated against several carboxymethylcellulases.
Kawaguchi
T
T
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture, Japan.
Sugimoto
K
K
Hayashi
H
H
Arai
M
M
D017485:1-Deoxynojirimycin / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002480:Cellulase / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013329:Structure-Activity Relationship
D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D017485:1-Deoxynojirimycin; D002480:Cellulase