title: Measurement of tumor oxygenation: a comparison between polarographic needle electrodes and a time-resolved luminescence-based optical sensor.
abstract: A novel oxygen sensor which does not rely on electrochemical reduction has been used to measure the oxygenation of the murine sarcoma F in a comparative study with an existing polarographic electrode that is available commercially. The prototype luminescence sensor yielded an oxygen distribution comparable with readings made using a pO2 histograph. The percentage of regions detected that had a pO2 less than 5 mm Hg was 79 and 75 using the Eppendorf pO2 histograph and the luminescence fiber optic sensor, respectively. These values were compatible with a measured radiobiologically hypoxic fraction of 67% in this tumor. The polarographic method detected more regions with a pO2 of 2.5 mm Hg or less (69%) compared with the optical sensor (50%) (P < 0.05). This could reflect differences in the oxygen use of the sensing devices. This initial assessment indicates the potential of a fiber-optic-based oxygen-monitoring system. Such a system should have several advantages including monitoring temporal oxygen changes in a given microregion and use with NMR procedures.
authors.affiliation: The Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, Middlesex, United Kingdom.
authors.forename: D R
authors.initials: DR
authors.lastname: Collingridge
authors.forename: W K
authors.initials: WK
authors.lastname: Young
authors.forename: B
authors.initials: B
authors.lastname: Vojnovic
authors.forename: P
authors.initials: P
authors.lastname: Wardman
authors.forename: E M
authors.initials: EM
authors.lastname: Lynch
authors.forename: S A
authors.initials: SA
authors.lastname: Hill
authors.forename: D J
authors.initials: DJ
authors.lastname: Chaplin
mesh_terms: D000818:Animals; D005260:Female; D005456:Fluorescent Dyes; D000860:Hypoxia / Q000175:diagnosis; D008163:Luminescent Measurements; D051379:Mice; D008808:Mice, Inbred CBA; D009942:Organometallic Compounds; D010100:Oxygen / Q000378:metabolism; D010618:Phenanthrolines; D011048:Polarography; D012428:Ruthenium; D012513:Sarcoma, Experimental / Q000098:blood supply* / Q000532:radiotherapy
chemical_list: D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D010618:Phenanthrolines; D012428:Ruthenium; C041132:tris(4,7-diphenyl-1,10-phenanthroline)ruthenium (II); D010100:Oxygen