title: Expression of the potato leafroll virus ORF0 induces viral-disease-like symptoms in transgenic potato plants.
abstract: The role of the open reading frame 0 (ORF0) of luteoviruses in the viral infection cycle has not been resolved, although the translation product (p28) of this ORF has been suggested to play a role in host recognition. To investigate the function of the potato leafroll luteovirus (PLRV) p28 protein, transgenic potato plants were produced containing the ORF0. In the lines in which the ORF0 transcripts could be detected by Northern (RNA) analysis, the plants displayed an altered phenotype resembling virus-infected plants. A positive correlation was observed between levels of accumulation of the transgenic transcripts and severity of the phenotypic aberrations observed. In contrast, potato plants transformed with a modified, untranslatable ORF0 sequence were phenotypically indistinguishable from wild-type control plants. These results suggest that the p28 protein is involved in viral symptom expression. Southern blot analysis showed that the transgenic plants that accumulated low levels of ORF0 transcripts detectable only by reverse transcription-polymerase chain reaction, contained methylated ORF0 DNA sequences, indicating down-regulation of the transgene provoked by the putatively unfavorable effects p28 causes in the plant cell.
authors.affiliation: DLO Research Institute for Plant Protection (IPO-DLO), Wageningen, The Netherlands. F.vanderWilk@ipo.dlo.nl
authors.forename: F
authors.initials: F
authors.lastname: van der Wilk
authors.forename: P
authors.initials: P
authors.lastname: Houterman
authors.forename: J
authors.initials: J
authors.lastname: Molthoff
authors.forename: F
authors.initials: F
authors.lastname: Hans
authors.forename: B
authors.initials: B
authors.lastname: Dekker
authors.forename: J
authors.initials: J
authors.lastname: van den Heuvel
authors.forename: H
authors.initials: H
authors.lastname: Huttinga
authors.forename: R
authors.initials: R
authors.lastname: Goldbach
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D017793:Luteovirus / Q000235:genetics* / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010641:Phenotype; D030821:Plants, Genetically Modified; D012367:RNA, Viral / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins