Chromosomal assignment of 311 sequences transcribed in human adult testis.
A total of 311 expressed sequence tags (ESTs) derived from human adult testis have been assigned to human chromosomes by Southern analysis of a monochromosome somatic cell hybrid panel. Over 70% of the ESTs show conservation to hamster and mouse DNA, and the overall distribution of transcripts correlates well with physical chromosome size and to a greater extent with male meiotic chromosome length. The notable exception is the X chromosome, for which the number of testis-derived ESTs is greatly underrepresented. This finding may reflect inactivation of the X chromosome during the meiotic phase of spermatogenesis and a consequent selection against large numbers of X-linked germ cell transcripts. Further analysis of the distribution of testis ESTs showed that the EST density remains significantly correlated with the recombination density of each autosome. Analysis of a comparable number (320) of brain EST autosome assignments showed no similar correlation. These data suggest a specific association between transcription in testis tissue and male meiotic recombination.
Jones
M H
MH
Department of Pathology, University of Cambridge, England, United Kingdom.
Zhang
Y
Y
Tirosvoutis
K N
KN
Davey
P M
PM
Webster
A R
AR
Walsh
D
D
Spurr
N K
NK
Affara
N A
NA
D000328:Adult; D000818:Animals; D002874:Chromosome Mapping; D006224:Cricetinae; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic
D018076:DNA, Complementary