title: Contrasting effects of ultraviolet-A and ultraviolet-B exposure on induction of contact sensitivity in human skin.
abstract: Ultraviolet-B (UVB), in addition to direct effects on DNA, induces immunological changes in the skin that predispose to the development of skin cancer. Whether ultraviolet-A (UVA) induces similar changes is unknown. This effect can be investigated in humans in vivo using epicutaneous antigens as a model of tumour antigens. Volunteers (n = 46) were randomly assigned to received no sensitization, sensitization with the allergen diphenylcyclopropenone (DPCP) on non-UV-exposed normal skin, or sensitization with DPCP on skin exposed to three minimal erythema doses (MED) of either UVA or UVB radiation 3 days before sensitization. Three weeks after sensitization all volunteers were challenged with five different concentrations of DPCP. The challenge reactions were scored clinically and the increase in skin thickness was measured using a micrometer. Sensitization on UVB-exposed skin reduced the immunization rate compared with sensitization on non-irradiated skin (P < 0.03) as previously described. In contrast, sensitization on skin exposed to three MED UVA radiation did not result in a decreased immunization rate compared with non-irradiated skin. These results indicate that in humans erythemagenic doses of UVA radiation do not reduce the immunization rates to epicutaneous allergens. Thus UVB and UVA irradiation have contrasting effects on cell-mediated immunity in humans. These findings may at least in part explain the less carcinogenic effect of UVA irradiation.
authors.affiliation: Department of Dermatology, University of Copenhagen, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Skov
authors.forename: H
authors.initials: H
authors.lastname: Hansen
authors.forename: J N
authors.initials: JN
authors.lastname: Barker
authors.forename: J C
authors.initials: JC
authors.lastname: Simon
authors.forename: O
authors.initials: O
authors.lastname: Baadsgaard
mesh_terms: D000293:Adolescent; D000328:Adult; D003521:Cyclopropanes / Q000008:administration & dosage / Q000276:immunology; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology; D004890:Erythema / Q000209:etiology / Q000276:immunology; D006801:Humans; D011897:Random Allocation; D012867:Skin / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D003521:Cyclopropanes; C029402:diphenylcyclopropenone