[Functional screening of p53 status in tumor cells using Saccharomyces cerevisiae].
We have detected both germ-line and somatic p53 mutations in lymphocytes, cell lines and tumor tissues using a functional analysis of p53 tumor suppressor gene based on yeast transcription assay. Through our screening projects of the p53 gene, a number of missense p53 mutations were identified as loss-of-function mutations. This method, previously termed FASAY, is rapid, sensitive, less-expensive and can be automated for screening both somatic and germ-line p53 mutations.
Jia
L Q
LQ
Dept. of Clinical Oncology, Tohoku University.
Ishioka
C
C
Osada
M
M
Gamo
M
M
Shimodaira
H
H
Suzuki
T
T
Kanamaru
R
R
D016158:Genes, p53 / Q000235:genetics / Q000502:physiology; D018095:Germ-Line Mutation; D006801:Humans; D008214:Lymphocytes; D009154:Mutation; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured