title: Genome organization, natural genetic engineering and adaptive mutation.
abstract: Bacterial evolution is considered in the light of molecular discoveries about genome organization, biochemical mechanisms of genetic change, and cellular control networks. Prokaryotic genetic determinants are organized as modular composites of coding sequences and protein-factor binding sites joined together during evolution. Studies of genetic change have revealed the existence of biochemical functions capable of restructuring the bacterial genome at various levels and joining together different sequence elements. These natural genetic engineering systems can be subject to regulation by signal transduction networks conveying information about the extracellular and intracellular environments. Mu-mediated araB-lacZ coding sequence fusions provide one example of adaptive mutation (increased formation of useful mutations under selection) and illustrate how physiological regulation can modulate the activity of a natural genetic engineering system under specific conditions.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637, USA. jsha@midway.uchicago.edu
authors.forename: J A
authors.initials: JA
authors.lastname: Shapiro
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome; D007763:Lac Operon / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012641:Selection, Genetic; D015398:Signal Transduction; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); D001616:beta-Galactosidase