title: Abnormal expression of the proliferating cell nuclear antigen (PCNA) in the spinal cord of the hypomyelinated Jimpy mutant mice.
abstract: In the present study, assessment of the expression of the proliferating cell nuclear antigen (PCNA), a nuclear acidic protein necessary for DNA replication that is expressed through the cell cycle, was used to investigate the proliferative capability of glial cells in the hypomyelinated Jimpy mutant mice. Spinal cords from 10-12 and 20-22 day Jimpy and normal animals were used for quantitative microscopic image analysis. Simultaneous demonstration of cycling cells and oligodendroglia, astroglia or microglia was achieved through the sequential combination of PCNA immunostaining and selective markers for these glial cells. Our results revealed that the density of PCNA-positive cells was higher in Jimpy than in normal spinal cords, this difference being more pronounced at 20-22 days than at 10-12 days and more so in white than in gray matter. In addition, Jimpy glial cells exhibited an abnormal PCNA expression, as demonstrated by quantification of the intensity of nuclear immunostaining. In comparison to normal animals, the percentage of PCNA-positive cells showing intensely stained nuclei was higher in Jimpy. About 50% of PCNA-positive cells in the Jimpy white matter were identified as cells from the oligodendrocyte line, 30% were microglial cells and 20% were astrocytes. The expression of PCNA in relation to the proliferative capability and possible cell cycle abnormalities of the different glial cell types in Jimpy is discussed.
authors.affiliation: Department of Cell Biology and Physiology, Faculty of Medicine, Autonomous University of Barcelona, Bellaterra, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Vela Hernández
authors.forename: I
authors.initials: I
authors.lastname: Dalmau
authors.forename: B
authors.initials: B
authors.lastname: González
authors.forename: B
authors.initials: B
authors.lastname: Castellano
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D037102:Lectins; D051379:Mice; D008810:Mice, Inbred C57BL; D008816:Mice, Jimpy; D017628:Microglia / Q000502:physiology; D004676:Myelin Basic Protein / Q000096:biosynthesis; D009186:Myelin Sheath / Q000502:physiology*; D009457:Neuroglia / Q000378:metabolism; D009836:Oligodendroglia / Q000502:physiology; D037121:Plant Lectins; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D037102:Lectins; D004676:Myelin Basic Protein; D037121:Plant Lectins; D018809:Proliferating Cell Nuclear Antigen; C034981:tomato lectin