Diversity of the 32-kilodalton protein gene may form a basis for species determination of potentially pathogenic mycobacterial species.
In this study, partial gene sequences of the mycobacterial 32-kDa protein gene were determined by PCR-based sequencing. A total of 50 strains representing 18 potentially pathogenic mycobacterial species were studied. In 10 cases, all three strains of the species studied were identical, and intraspecies variability was found in 6 cases. Thus, the 32-kDa protein gene may be a good target for identification of mycobacteria by PCR-based sequencing.
Soini
H
H
Department in Turku, National Public Health Institute, Finland.
Viljanen
M K
MK
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009161:Mycobacterium / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S