Inhibitory effects of caffeic acid ethyl ester on H2O2-induced cytotoxicity and DNA single-strand breaks in Chinese hamster V79 cells.
Cytotoxicity and DNA single-strand breaks caused by H2O2 were assessed by a colony formation assay and a DNA precipitation assay, respectively, with Chinese hamster V79 cells. In both assays, caffeic acid ethyl ester showed protective effects. The structure-activity relationship showed that the o-dihydroxy structure of caffeic acid ethyl ester was essential for the protective effects.
Nakayama
T
T
School of Food and Nutritional Sciences, University of Shizuoka, Japan.
Yamada
M
M
Osawa
T
T
Kawakishi
S
S
D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D004347:Drug Interactions; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D013329:Structure-Activity Relationship
D002109:Caffeic Acids; C099085:ethyl ferulate; C032773:ethyl caffeate; D004247:DNA; D006861:Hydrogen Peroxide