title: Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization.
abstract: The peroxidase-mediated deposition of hapten- and fluorochrome-labeled tyramides has recently been shown to increase the sensitivity of immunofluorescence and fluorescence in situ hybridization techniques. We have evaluated a number of red, green, and blue fluorescent tyramides for detection of antigens in tissue sections and cytospin preparations and for the detection of hapten- and horseradish peroxidase-labeled probes hybridized in situ to cells and chromosomes. With few exceptions, all fluorescent tyramide-based methods provided a considerable increase in sensitivity compared to conventional immunofluorescence and FISH methods.
authors.affiliation: Department of Cytochemistry and Cytometry, Sylvius Laboratory, Leiden University, The Netherlands.
authors.forename: R P
authors.initials: RP
authors.lastname: van Gijlswijk
authors.forename: H J
authors.initials: HJ
authors.lastname: Zijlmans
authors.forename: J
authors.initials: J
authors.lastname: Wiegant
authors.forename: M N
authors.initials: MN
authors.lastname: Bobrow
authors.forename: T J
authors.initials: TJ
authors.lastname: Erickson
authors.forename: K E
authors.initials: KE
authors.lastname: Adler
authors.forename: H J
authors.initials: HJ
authors.lastname: Tanke
authors.forename: A K
authors.initials: AK
authors.lastname: Raap
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051381:Rats; D012680:Sensitivity and Specificity; D014439:Tyramine / Q000737:chemistry*; D014746:Vimentin / Q000032:analysis
chemical_list: D000941:Antigens; D005456:Fluorescent Dyes; D014746:Vimentin; D006735:Horseradish Peroxidase; D014439:Tyramine