Direct assay of tinidazole in human serum by micellar liquid chromatography.
A liquid chromatographic procedure for the direct determination of tinidazole in human serum is presented. It includes the use of a micellar mobile phase consisting of SDS (5.10(-2) M): propan-1-ol; (94:6, v/v) and a mu Bondapak CN column with UV detection at 320 nm. No solvent extraction or deproteinization are necessary. The linearity (0.1-10 mg L), the precision (3%), the reproducibility (1.3%), the recovery (99%), and the detection limit (0.1 mg L) in the tinidazole determination are comparable and sometimes greater than the corresponding tinidazole parameters when deproteinization and conventional reversed-phase HPLC are used. One hundred injections of serum samples do not affect the column life. The procedure is applied to ascertain the pharmacokinetics of 10 mg/kg of tinidazole.
Habel
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Insitut de Chimie, USTHB, Alger, Algeria.
Guermouche
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Guermouche
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D000097:Acetonitriles; D000994:Antitrichomonal Agents / Q000097:blood*; D001798:Blood Proteins; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008823:Micelles; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014011:Tinidazole / Q000097:blood*
D000097:Acetonitriles; D000994:Antitrichomonal Agents; D001798:Blood Proteins; D008823:Micelles; D014011:Tinidazole; C032159:acetonitrile