Cyclin A message stability varies with the cell cycle.
Progression through the cell cycle in somatic eukaryotic cells is regulated by variations in the levels of cyclin proteins. These protein levels are in turn regulated by cyclical oscillations in their mRNA levels. We show here that regulation of RNA stability plays a role in the mechanisms underlying cell cycle progression. Both cyclin A and B1 messages are expressed at high levels in G2-M and at low levels in early G1. The half-lives of their messages mirror this pattern, long in G2-M (>8 h) and short in early G1 (1-2 h). However, there is evidence of specificity to these changes, because the cyclin A message becomes stable at the G1-S boundary, whereas the cyclin B1 message is unstable until later in S phase. Furthermore, although cyclin B1 mRNA levels are lowered after irradiation because of enhanced instability, cyclin A mRNA levels and message stability are unaffected by irradiation. Additional evidence of specificity was found in an analysis of cyclin E mRNA stability, which remains constant through the cell cycle, although the cyclin E message displays cell cycle-dependent changes in expression. These studies suggest that specific alterations in RNA stability are an important component in regulating the expression of cyclins A and B1 and hence in controlling the cell cycle.
Maity
A
A
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
McKenna
W G
WG
Muschel
R J
RJ
D015152:Blotting, Northern; D002453:Cell Cycle / Q000502:physiology*; D019926:Cyclin B; D056744:Cyclin B1; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D005786:Gene Expression Regulation; D006207:Half-Life; D006367:HeLa Cells; D006801:Humans; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors
C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D016213:Cyclins; D012333:RNA, Messenger