A disc plate assay for characterization of the effect of interaction between polyenes and azoles on growth of Candida albicans CA12.
A simple, reproducible and efficient assay was described for visual demonstration that triazole fluconazole interfered with the anti-Candida albicans activity of polyene amphotericin B. The assay also indicated the existence of more than one antifungal mechanism involving imidazole ketoconazole.
Cao
B
B
Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
Tsang
P W
PW
Tsang
D N
DN
Samaranayake
L P
LP
Wang
J
J
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D004336:Drug Antagonism; D015725:Fluconazole / Q000494:pharmacology*; D007654:Ketoconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*
D000935:Antifungal Agents; D000666:Amphotericin B; D015725:Fluconazole; D007654:Ketoconazole