title: Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB), a neural member of the EGF family of differentiation factors, is implicated in neurite formation.
abstract: Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB) was identified by combining binding assays with immunological screens in the chicken nervous system as a novel member of the EGF family of differentiation factors. cDNA cloning indicates that CALEB is a multidomain protein that consists of an NH2-terminal glycosylation region, a leucine-proline-rich segment, an acidic box, a single EGF-like domain, a transmembrane, and a short cytoplasmic stretch. In the developing nervous system, CALEB is associated with glial and neuronal surfaces. CALEB is composed of a 140/130-kD doublet, an 80-kD band, and a chondroitinsulfate-containing 200-kD component. The latter two components are expressed in the embryonic nervous system and are downregulated in the adult nervous system. CALEB binds to the extracellular matrix glycoproteins tenascin-C and -R. In vitro antibody perturbation experiments reveal a participation of CALEB in neurite formation in a permissive environment.
authors.affiliation: Max-Delbruck-Centrum fur Molekulare Medizin, Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schumacher
authors.forename: H
authors.initials: H
authors.lastname: Volkmer
authors.forename: F
authors.initials: F
authors.lastname: Buck
authors.forename: A
authors.initials: A
authors.lastname: Otto
authors.forename: A
authors.initials: A
authors.lastname: Tarnók
authors.forename: S
authors.initials: S
authors.lastname: Roth
authors.forename: F G
authors.initials: FG
authors.lastname: Rathjen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D030161:Avian Proteins; D001369:Axons / Q000378:metabolism; D001923:Brain Chemistry; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens; D002809:Chondroitin Sulfates / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000276:immunology / Q000378:metabolism* / Q000502:physiology*; D007930:Leucine; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism* / Q000502:physiology*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism* / Q000502:physiology*; D016501:Neurites / Q000276:immunology / Q000502:physiology*; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D011946:Receptors, Antigen / Q000378:metabolism; D012160:Retina / Q000378:metabolism; D013379:Substrate Specificity; D019063:Tenascin / Q000378:metabolism
chemical_list: D019138:Antibodies, Blocking; D030161:Avian Proteins; C104596:CALEB protein, chicken; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D008562:Membrane Glycoproteins; C102222:NRCAM protein, Gallus gallus; D009419:Nerve Tissue Proteins; D011946:Receptors, Antigen; D019063:Tenascin; C028641:Tn receptor; D004815:Epidermal Growth Factor; D002809:Chondroitin Sulfates; D007930:Leucine