title: Decreased N-myc expression in human medulloblastoma cell lines during differentiation.
abstract: Medulloblastoma is the most common primitive neuroectodermal tumor of the central nervous system, and shares some biochemical and immunological features with neuroblastoma. It could be suggested that N-myc expression in medulloblastoma is correlated with primitiveness or cell differentiation, as in neuroblastoma. N-myc expression in two human medulloblastoma cell lines (ONS-76 and -81) was investigated during drug-induced differentiation by immunocytochemistry and Western blotting. The growth rates of the two medulloblastoma cell lines showed a marked decrease and morphological differentiation occurred after treatment with 1 mM dibutyryl cyclic-adenosine 3',5'-monophosphate (dbt-cAMP). The decrease in N-myc expression with differentiation of cells was confirmed by immunocytochemistry and Western blotting. We believe that decreased N-myc expression is correlated with cell differentiation, and a decrease in the primitiveness of medulloblastoma cells.
authors.affiliation: Department of Neurosurgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Moriuchi
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: Y
authors.initials: Y
authors.lastname: Miyao
authors.forename: H
authors.initials: H
authors.lastname: Kishima
authors.forename: M
authors.initials: M
authors.lastname: Okawa
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D002454:Cell Differentiation; D002455:Cell Division; D006801:Humans; D007150:Immunohistochemistry; D008527:Medulloblastoma / Q000737:chemistry / Q000473:pathology*; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D016271:Proto-Oncogene Proteins c-myc