title: Difference in gene expression for matrix metalloproteinase-1 between early and advanced hepatocellular carcinomas.
abstract: The histological observation that well-differentiated cancer cells in early hepatocellular carcinoma (HCC) invade portal tracts and/or fibrous bands and that these fibrous tissues then disappear suggests the participation of matrix metalloproteinase-1 (MMP-1) in the degradation of fibrous tissue. To confirm this hypothesis, the authors investigated the localization of both the MMP-1 protein and its messenger RNA (mRNA) in early HCC immunohistochemically and by in situ hybridization using complementary DNA (cDNA) and synthetic antisense probe of MMP-1; they then compared the results with those in advanced HCC. MMP-1 gene transcripts and protein were observed in well-differentiated cancer cells of early HCC but not in moderately or poorly differentiated cancer cells. Thus, cancer cells producing MMP-1 in early HCC may destroy the portal tract tissue adjacent to the cancer lesion and/or the fibrous band of cirrhosis. These results seem to have demonstrated a difference in the mechanism of cancer growth and invasion between early and advanced HCCs.
authors.affiliation: Department of Community Health, School of Medicine, Tokai University, Isehara City, Kanagawa, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Okazaki
authors.forename: N
authors.initials: N
authors.lastname: Wada
authors.forename: M
authors.initials: M
authors.lastname: Nakano
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Takasaki
authors.forename: M
authors.initials: M
authors.lastname: Doi
authors.forename: K
authors.initials: K
authors.lastname: Kameyama
authors.forename: Y
authors.initials: Y
authors.lastname: Otani
authors.forename: K
authors.initials: K
authors.lastname: Kubochi
authors.forename: M
authors.initials: M
authors.lastname: Niioka
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology*; D017364:Collagenases / Q000032:analysis* / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology*; D008297:Male; D020781:Matrix Metalloproteinase 1; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D009363:Neoplasm Proteins; D012333:RNA, Messenger; D017364:Collagenases; D020781:Matrix Metalloproteinase 1