title: Report on a patient with paroxysmal cold hemoglobinuria.
abstract: Antibodies against the human blood group P antigen (anti-P alloantibodies) agglutinate phenotype P1 and P2 erythrocytes treated with papain at 4 degrees C but not phenotype PK and p erythrocytes. This condition is referred to as an autoimmune disease of paroxysmal cold hemoglobinuria (PCH), and the anti-P specificity is found in the cold hemagglutinins. Serum from a patient suspected to be suffering from PCH by the cold auto-agglutination properties was tested for anti-P specificity, using papain-treated O blood group erythrocytes. The serum-mediated hemagglutination and the serum and complement-mediated immunohemolysis were inhibited by globoside (P antigen GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc-ceramide) and Forssman glycosphingolipid (GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc-ceramide). Therefore, we concluded that she had PCH. She was completely cured 6 months later.
authors.affiliation: Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
authors.forename: P R
authors.initials: PR
authors.lastname: Subbarayan
authors.forename: T
authors.initials: T
authors.lastname: Shichishima
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Maruyama
authors.forename: M
authors.initials: M
authors.lastname: Naiki
authors.forename: S
authors.initials: S
authors.lastname: Maruyama
mesh_terms: D005260:Female; D005915:Globosides / Q000276:immunology*; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D006801:Humans; D007518:Isoantibodies / Q000097:blood*; D008875:Middle Aged
chemical_list: D005915:Globosides; D007518:Isoantibodies