A mouse cytomegalovirus glycoprotein retains MHC class I complexes in the ERGIC/cis-Golgi compartments.
The principle by which mouse cytomegalovirus blocks antigen presentation in the MHC class I pathway was investigated. The responsible gene m152, encoding a type I transmembrane glycoprotein of 40 kDa, is a member of a gene family located in the right-hand terminal region of the 230 kb virus genome. Expression of m152 in murine and human cells arrested the export of mouse class I complexes from the ER-Golgi intermediate compartment/cis-Golgi compartment and inhibited lysis by cytotoxic T cells. The plasma membrane transport of human MHC class I molecules was not affected. The deletion of the cytoplasmic tail of gp40 did not lift its effect on class I molecule export, indicating that this protein differs in its functions from known immunosubversive viral gene products and represents a novel principle by which a herpesvirus shuts off MHC class I function.
Ziegler
H
H
Max von Pettenkofer Institut, Ludwig-Maximilians-Universitat, Munchen, Federal Republic of Germany.
Thale
R
R
Lucin
P
P
Muranyi
W
W
Flohr
T
T
Hengel
H
H
Farrell
H
H
Rawlinson
W
W
Koszinowski
U H
UH
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000382:microbiology; D001692:Biological Transport; D002451:Cell Compartmentation; D003593:Cytoplasm / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D005814:Genes, Viral; D006056:Golgi Apparatus / Q000378:metabolism*; D006183:H-2 Antigens / Q000378:metabolism*; D006566:Herpesviridae Infections / Q000276:immunology*; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D007739:L Cells; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D018146:Muromegalovirus / Q000276:immunology*; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication
D006183:H-2 Antigens; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C104783:m152 protein, cytomegalovirus; D006596:Hexosaminidases