Intercalation of ethidium and analogues with nucleic acids: a molecular orbital study.
Semiempirical calculations suggest that the intercalation complexes of phenanthridinium cations 1-4 with G-C/C-G and 1 with A-U/U-A are stabilized by frontier orbital interactions between the LUMO of the intercalator and the HOMOs of the adjacent purine bases. The charge on the ring nitrogen of 1-4 appears to be necessary for the orbital interactions, lowering the LUMO, facilitating mixing of this orbital with the HOMOs of the adjacent purine bases to give an extended HOMO stabilizing the complex and resulting in the bathochromic shift in the electron absorption spectrum. Noncationic phenanthridine 5 shows no frontier orbital interactions in the forced intercalation complex with G-C/C-G. The results of the calculations parallel experimental T(m) values.
Patterson
S E
SE
Department of Chemistry, Georgia State University, Atlanta 30303, USA.
Coxon
J M
JM
Strekowski
L
L
D004996:Ethidium / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D013816:Thermodynamics
D007364:Intercalating Agents; D009696:Nucleic Acids; D004996:Ethidium