The applicability of hematoxylin-eosin staining plus fluorescence or confocal laser scanning microscopy to the study of elastic fibers in cartilages.
This study focuses on the use of hematoxylin-eosin staining plus fluorescence microscopy for the investigation of elastic fibers in some elastic cartilages. We have observed that elastic fibers are consistently imaged by the proposed procedure and the resolution attained is similar to that obtained with the classical Weigert's fuchsin-resorcin. The results also demonstrate that elastin autofluorescence gives little or no contribution to the final fluorescence and that the use of the confocal laser scanning microscope adds to the resolution, permits the use of thicker sections and reveals of minute structural at features. We conclude that this is a relevant tool in elastin research.
de Carvalho
H F
HF
Department of Cell Bilogy, UNICAMP, Campinas, Brazil.
Taboga
S R
SR
D000818:Animals; D002356:Cartilage / Q000648:ultrastructure; D004285:Dogs; D004547:Elastic Tissue / Q000648:ultrastructure*; D004801:Eosine Yellowish-(YS) / Q000008:administration & dosage; D005456:Fluorescent Dyes / Q000008:administration & dosage; D006416:Hematoxylin / Q000008:administration & dosage; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D012118:Resorcinols / Q000008:administration & dosage; D012394:Rosaniline Dyes / Q000008:administration & dosage
D005456:Fluorescent Dyes; D012118:Resorcinols; D012394:Rosaniline Dyes; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin