Mouse p150Glued (dynactin 1) cDNA sequence and evaluation as a candidate for the neuromuscular disease mutation mnd2.
p150Glued (Dynactin 1) is a component of the dynactin complex that is essential for retrograde axonal transport in neurons. The mouse p150Glued cDNA was isolated from mouse brain by RT-PCR. The complete sequence of the full length mouse cDNA was determined, including 19 bp of 5' UTR, 3843 bp of coding sequence, and 162 bp of 3' UTR. The predicted protein has a molecular mass of 142 kDa and exhibits 95% amino acid sequence identity to the predicted amino acid sequence of human p150Glued (DCTN1). The mouse Dctn1 gene was previously mapped in the central region of mouse chromosome 6, close to the neuromuscular disease gene mnd2. Northern blot analysis, complete sequencing of the cDNA, Western blot, and functional tests of the protein did not detect any abnormalities of p150Glued in mnd2 mice.
Jang
W
W
Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48108-0618, USA.
Weber
J S
JS
Tokito
M K
MK
Holzbaur
E L
EL
Meisler
M H
MH
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary; D000072159:Dynactin Complex; D004398:Dyneins / Q000235:genetics*; D005069:Evaluation Studies as Topic; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008868:Microtubule Proteins / Q000235:genetics*; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009468:Neuromuscular Diseases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
C000606360:DCTN1 protein, human; D018076:DNA, Complementary; C000606364:Dctn1 protein, mouse; D000072159:Dynactin Complex; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D004398:Dyneins