Cytotoxicity and immunosuppressive activity of withanolides from Discopodium penninervium.
The cytotoxicity and immunosuppressive activities of jaborosalactone-L and three 16 alpha-oxygenated withanolides from the leaves of Discopodium penninervium were studied. Jaborosalactone-L showed cytotoxicity only to the murine macrophage cell line, RAW 264.7, but the 16 alpha-oxygenated withanolides exhibited cytotoxicity to both human (COR-L23 and ECV 304) and murine (L929 and RAW 264.7) carcinoma cell lines with IC50 values ranging from 1.2-150 microM. The non-toxic concentrations of these withanolides were also found to reduce the proliferative response of both inactive and E. coli lipopolysaccharide and concanavalin A activated rat spleen cells in vitro.
Habtemariam
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Department of Physiology and Pharmacology, University of Strathclyde, Glasgow, U.K.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D007166:Immunosuppressive Agents; D004875:Ergosterol