The primary structure of the Cl(-)-translocating ATPase, b subunit of Acetabularia acetabulum, which belongs to the F-type ATPase family.
The genes possibly encoding the b subunit (50 kDa) of the Cl(-)-translocating ATPase of Acetabularia acetabulum were cloned from total RNA and from poly(A)+ RNA and sequenced. The deduced amino acid sequence of the open reading frame consisted of 478 amino acids and showed high similarity to the beta subunit of chloroplast F1-ATPase. Gene fragments encoding the putative beta subunit of chloroplast F1- (273 bp) and mitochondrial F1-ATPases (332 bp) were also cloned from A. acetabulum and sequenced, respectively. The deduced amino acid sequence of the chloroplast F1-ATPase showed 92.5% identity to be primary structure of the b subunit of the Cl(-)-translocating ATPase, while the nucleotide sequences were 79.9% identical. The deduced amino acid sequence of the latter was 77.3% identical to that of the b subunit of the Cl(-)-translocating ATPase and the nucleotide sequences were 67.5% identical. By Northern analysis, these three beta-like genes were demonstrated to be transcribed with different sizes of RNA species. A putative chloroplast F1-beta fragment also hybridized with chloroplast DNA isolated from the organism.
Moritani
C
C
Faculty of Health and Welfare Science, Okayama Prefectural University, Soja, Japan.
Ohhashi
T
T
Kadowaki
H
H
Tagaya
M
M
Fukui
T
T
Lottspeich
F
F
Oesterhelt
D
D
Ikeda
M
M
D000076:Acetabularia / Q000737:chemistry*; D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D027321:Anion Transport Proteins; D001426:Bacterial Proteins / Q000276:immunology; D001483:Base Sequence; D001693:Biological Transport, Active; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000145:classification / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D027321:Anion Transport Proteins; D001426:Bacterial Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D000251:Adenosine Triphosphatases; C031652:anion-sensitive ATPases