99Tcm-phenylene imine phenol as a potential leukocyte labelling agent.
The aim of this study was to evaluate 99Tcm-labelled phenylene imine phenol (99Tcm-Ph(IP)2) as a leukocyte label. The 99Tcm complex exhibits a neutral lipophilic character. The radiochemical purity of the 99Tcm-Ph(IP)2 was high both soon after labelling (94.5 +/- 1.2%, n = 7) and at 24 h (89.7 +/- 1.0%, n = 7). In a comparative study, the labelling efficiency of 99Tcm-Ph(IP)2 and 99Tcm-hexamethylpropylene amine oxime (99Tcm-HMPAO) labelled leukocytes was 47.1 +/- 3.5% (n = 7) and 58.5 +/- 7.0% (n = 7) respectively. The viability of labelled leukocytes was greater than 90% for both 99Tcm complexes. The elution ratios of labelled leukocytes with 99Tcm-Ph(IP)2 at 1, 4 and 24 h were 10.1 +/- 3.5%, 17.2 +/- 2.7% and 23.0 +/- 2.4% (n = 7) respectively, while those with 99Tcm-HMPAO were 10.8 +/- 1.2%, 18.3 +/- 2.0% and 37.0 +/- 3.7% (n = 7) respectively. In conclusion, 99Tcm-Ph(IP)2, with good stability and significant leukocyte labelling efficiency, is a potential leukocyte label.
Huang
W T
WT
Department of Nuclear Science, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.
Lo
J M
JM
Kao
C H
CH
Wang
S J
SJ
D000818:Animals; D002470:Cell Survival; D004396:Coloring Agents; D006801:Humans; D007097:Imines; D066298:In Vitro Techniques; D007700:Kinetics; D007962:Leukocytes / Q000166:cytology; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D010091:Oximes / Q000493:pharmacokinetics; D051381:Rats; D019690:Technetium Tc 99m Exametazime; D014018:Tissue Distribution; D014343:Trypan Blue
D004396:Coloring Agents; D007097:Imines; D015609:Organotechnetium Compounds; D010091:Oximes; C105828:technetium Tc 99m phenylene imine phenol; D019690:Technetium Tc 99m Exametazime; D014343:Trypan Blue