Phage-display libraries of murine and human antibody Fab fragments.
We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.
Engberg
J
J
Royal Danish School of Pharmacy, Department of Biology, Copenhagen, Denmark. je@charon.dfh.ok
Andersen
P S
PS
Nielsen
L K
LK
Dziegiel
M
M
Johansen
L K
LK
Albrechtsen
B
B
D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D051379:Mice
D007140:Immunoglobulin Fab Fragments