title: Substrate specificities of alpha-galactosidases from yeasts.
abstract: Twenty-nine strains of yeasts, which are capable of using galactose, melibiose, or raffinose, were screened for alpha-galactosidase production. Among the strains, 5 produced intracellular and extracellular alpha-galactosidases, and 2 produced only intracellular enzyme. Substrate specificities of these enzymes were explored using 6(3)-alpha-D-galactosyl-1,4-beta-D-mannotriose and 6(3)-alpha-D-galactosyl-1,4-beta-D-mannotetraose. All enzymes liberated the terminal galactose from 6(3)-alpha-D-galactosyl-1,4-beta-D-mannotriose, but did not the stub galactose from 6(3)-alpha-D-galactosyl-1,4-beta-D-mannotetraose.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: C H
authors.initials: CH
authors.lastname: Tan
authors.forename: T
authors.initials: T
authors.lastname: Shimokawa
authors.forename: H
authors.initials: H
authors.lastname: Turakainen
authors.forename: I
authors.initials: I
authors.lastname: Kusakabe
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism*; D009598:Nitrophenylgalactosides / Q000378:metabolism; D013379:Substrate Specificity; D015003:Yeasts / Q000201:enzymology*; D000519:alpha-Galactosidase / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D009598:Nitrophenylgalactosides; C059200:4-nitrophenylgalactoside; D000519:alpha-Galactosidase