Interaction between Ustilago maydis REC2 and RAD51 genes in DNA repair and mitotic recombination.
A gene encoding a Ustilago maydis Rad51 orthologue has been isolated, rad51-1, a mutant constructed by disrupting the gene, was as sensitive to killing by ultraviolet light and gamma radiation as the rec2-1 mutant and slightly more sensitive to killing by methyl methanesulfonate. There was no suppression of killing by ultraviolet light when a rec2-1 strain was transformed with a multicopy plasmid containing RAD51, nor was there suppression when rad51-1 was transformed with a multicopy plasmid containing REC2. Recombination proficiency as measured by a gap repair assay was diminished in both rec2-1 and rad51-1 strains. In rec2-1 the frequency of recombination was decreased, but the spectrum of events was similar to that observed in wild type, while in rad51-1 the frequency as well as the spectrum of recombination events were different. Studies with the rec2-1 rad51-1 double mutant indicated that there was epistasis in the action of REC2 and RAD51 in certain repair and recombination functions, but some measure of independent action in other functions.
Ferguson
D O
DO
Hearst Microbiology Research Center, Department of Microbiology, Cornell University Medical College, New York, New York 10021, USA.
Rice
M C
MC
Rendi
M H
MH
Kotani
H
H
Kmiec
E B
EB
Holloman
W K
WK
D000595:Amino Acid Sequence; D001483:Base Sequence; D015825:Chromosomes, Fungal; D004260:DNA Repair; D004271:DNA, Fungal; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005720:Gamma Rays; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D008938:Mitosis; D008969:Molecular Sequence Data; D010957:Plasmids; D051135:Rad51 Recombinase; D011995:Recombination, Genetic; D014466:Ultraviolet Rays; D014588:Ustilago / Q000235:genetics*
D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C088888:REC2 protein, Ustilago maydis; D051135:Rad51 Recombinase