title: Transfection techniques for producing recombinant baculoviruses.
abstract: The production of recombinant baculoviruses usually employs cotransfection of insect tissue-culture cells with viral and transfer-plasmid DNAs. The preparation and storage of viral and plasmid DNAs suitable for optimal transfection of insect cells are discussed. Electroporation, calcium-phosphate, and lipofection transfection techniques are presented with a discussion of their relative advantages. The rates of recombinant virus formation are compared using viral infection/plasmid transfection protocols versus cotransfection of cells with transfer-plasmid and viral DNAs.
authors.affiliation: Boyce Thompson Institute at Cornell, Ithaca, NY 14853, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Trotter
authors.forename: H A
authors.initials: HA
authors.lastname: Wood
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D005822:Genetic Vectors; D011995:Recombination, Genetic; D018411:Spodoptera; D014162:Transfection / Q000379:methods*