title: Subcellular localization of myosin V in nerve growth cones and outgrowth from dilute-lethal neurons.
abstract: Myosin V-null mice (dilute-lethal mutants) exhibit apparent neurological defects that worsen from birth until death in the third postnatal week. Although myosin V is enriched in brain, the neuronal function of myosin V is unclear and the underlying cause of the neurological defects in these mice is unknown. To aide in understanding myosin V function, we examined the distribution of myosin V in the rodent superior cervical ganglion (SCG) growth cone, a well characterized neuronal structure in which myosin V is concentrated. Using affinity purified, myosin V-specific antibodies in immunofluorescence and immunoelectron microscopy, we observed that myosin V is concentrated in organelle-rich regions of the growth cone. Myosin V is present on a distinct population of small (50-100 nm) organelles, and on actin filaments and the plasma membrane. Myosin V-associated organelles are present on both microtubules and actin filaments. These results indicate that myosin V may be carried as a passenger on organelles that are transported along microtubules, and that these organelles may also be capable of movement along actin filaments. In addition, we found no abnormalities in outgrowth, morphology, or cytoskeletal organization of SCG growth cones from dilute-lethal mice. These results indicate that myosin V is not necessary for the traction force needed for growth cone locomotion, for organization of the actin cytoskeleton, or for filopodial dynamics.
authors.affiliation: Department of Anatomy and Neurobiology, Washington University, St Louis, Missouri 63110, USA.
authors.forename: L L
authors.initials: LL
authors.lastname: Evans
authors.forename: J
authors.initials: J
authors.lastname: Hammer
authors.forename: P C
authors.initials: PC
authors.lastname: Bridgman
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002462:Cell Membrane; D003599:Cytoskeleton / Q000378:metabolism; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D009218:Myosins / Q000032:analysis* / Q000276:immunology; D009474:Neurons / Q000737:chemistry*; D017830:Octoxynol / Q000737:chemistry; D015388:Organelles; D051381:Rats; D013347:Subcellular Fractions; D017783:Superior Cervical Ganglion / Q000737:chemistry*
chemical_list: D000199:Actins; D000906:Antibodies; D017830:Octoxynol; D009218:Myosins