title: Adipose tissue lipolysis and hormone-sensitive lipase expression during very-low-calorie diet in obese female identical twins.
abstract: Eight pairs of obese female monozygotic twins were subjected to a 4-week, very-low-calorie diet (VLCD) that induced a decrease in mean body mass index from 32.9 +/- 1.1 to 29.7 +/- 1.1 kg/m2. Infusion of the beta-adrenergic agonist, isoproterenol, induced an increase in plasma levels of nonesterified fatty acids and glycerol that was more pronounced during than before VLCD. sc fat biopsies were obtained before and during VLCD to study adipocyte lipolysis. beta-adrenergic sensitivity was moderately improved during VLCD. Basal and stimulated lipolyses, and hormone-sensitive lipase activity and protein levels were increased during VLCD. Before VLCD, intrapair resemblance was found for basal and stimulated lipolysis rates. In response to the treatment, intrapair resemblance was observed for basal lipolysis and for lipolysis stimulated with agents acting on plasma membrane receptors. These results suggest that the increase of basal lipolysis during VLCD is caused by an increase of hormone-sensitive lipase expression. They support the notion that the genotype may play a role in regulating the changes of adipose tissue lipolysis rates observed during VLCD.
authors.affiliation: Fourth Department of Medicine, Charles University, Prague 2, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Stich
authors.forename: I
authors.initials: I
authors.lastname: Harant
authors.forename: I
authors.initials: I
authors.lastname: De Glisezinski
authors.forename: F
authors.initials: F
authors.lastname: Crampes
authors.forename: M
authors.initials: M
authors.lastname: Berlan
authors.forename: M
authors.initials: M
authors.lastname: Kunesova
authors.forename: V
authors.initials: V
authors.lastname: Hainer
authors.forename: M
authors.initials: M
authors.lastname: Dauzats
authors.forename: D
authors.initials: D
authors.lastname: Rivière
authors.forename: M
authors.initials: M
authors.lastname: Garrigues
authors.forename: C
authors.initials: C
authors.lastname: Holm
authors.forename: M
authors.initials: M
authors.lastname: Lafontan
authors.forename: D
authors.initials: D
authors.lastname: Langin
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D018663:Adrenergic Agents / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000328:Adult; D004038:Diet, Reducing; D002149:Energy Intake; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy / Q000378:metabolism*; D002787:Sterol Esterase / Q000378:metabolism*; D014430:Twins, Monozygotic
chemical_list: D018663:Adrenergic Agents; D000318:Adrenergic beta-Agonists; D002787:Sterol Esterase; D007545:Isoproterenol; D004837:Epinephrine