Peroxidase activity may play a role in the cytotoxic effect of indole acetic acid.
Peroxidase activity in neutrophils is higher than in thioglycollate macrophages, while in lymphocytes this enzyme activity is very low. Indole-3-acetic acid is oxidized by peroxidase and the role of this enzyme in the cytotoxic effect of the compound was evaluated by measuring oxygen consumption, light emission and cell death in neutrophils, macrophages and lymphocytes. The increase in light emission, oxygen consumption and rate of cell death in cells cultured in the presence of indole-3-acetic acid presented a direct correlation with the peroxidase activity of the cells as follows: neutrophils > thioglycollate macrophages > resident macrophages > lymphocytes. Indeed, in lymphocytes that possess very low peroxidase activity, indole-3-acetic acid did not result in an increase in light emission or oxygen consumption and it was not cytotoxic.
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Department of Biochemistry, University of São Paulo, Brazil. mpdemelo@BMB.ICB1.USP.BR
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D000818:Animals; D002478:Cells, Cultured; D007210:Indoleacetic Acids / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D009195:Peroxidase / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D009195:Peroxidase