Isolation of monochromosomal hybrids for mouse chromosomes 3, 6, 10, 12, 14, and 18.
Mouse/human somatic cell hybrids constitute a valuable resource for both genetic and physical mapping. In this report, we describe the production and characterization of a series of six monochromosomal hybrids generated by fusion of murine micro-cells with intact human recipient cells. The presence of each mouse chromosome was characterized by PCR analysis and the integrity of the mouse chromosome retained in the hybrids confirmed by fluorescence in situ hybridization (FISH) analysis.
Sabile
A
A
Unité de Génétique Moléculaire Murine, Institut Pasteur, Paris, France.
Poras
I
I
Cherif
D
D
Goodfellow
P
P
Avner
P
P
D000818:Animals; D002460:Cell Line; D002875:Chromosomes; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016133:Polymerase Chain Reaction