Pulsed-field gel electrophoresis is more discriminating than multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis for typing pyogenic streptococci.
The SmaI restriction endonuclease digestion patterns of chromosomal DNAs from 99 pyogenic streptococci belonging to Lancefield group A (41 Streptococcus pyogenes), group C (seven S.dysgalactiae, 11 "S. equisimilis", three S. equi, eight S. zooepidemicus) and group G (25 human group G Streptococcus, four S. canis) were analyzed by pulsed-field gel electrophoresis (PFGE), and the results were compared with those previously obtained by multilocus enzyme electrophoresis (MLEE) and random amplified polymorphic DNA analysis (RAPD). PFGE revealed 93 distinct types among the 99 strains, and no patterns were common to strains of different species. The discriminatory power of PFGE was greater than that of MLEE and RAPD for groups A and G streptococci. The polymorphism among group C streptococci was similar with the three techniques. PFGE is, therefore, the most efficacious method for epidemiological typing of pyogenic streptococci.
Bert
F
F
Hôpital Beaujon, service de Microbiologie, 100 boulevard du Général Leclerc, 92210 Clichy, France.
Branger
C
C
Lambert-Zechovsky
N
N
D004269:DNA, Bacterial / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D017720:Molecular Epidemiology / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D013297:Streptococcus pyogenes / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial