DNAzol: a reagent for the rapid isolation of genomic DNA.
In this report, we present DNAzol, a patent-pending DNA isolation reagent containing guanidine thiocyanate and a detergent mixture. It is a complete, nontoxic and ready-to-use reagent for the isolation of genomic DNA from various biological sources. In the DNAzol protocol, a biological sample is homogenized (or lysed) in DNAzol, and the DNA is precipitated with ethanol, washed and dissolved in 8 mM NaOH. Following pH adjustment, the DNA can be used immediately for analysis or stored at 4 degrees C. The entire isolation can be completed in 20-30 min, and a wide range of DNA molecules can be isolated including genomic DNA and DNA fragments down to 0.1 kb in length. If necessary, samples can be stored in DNAzol at room temperature for extended periods of time. The isolated DNA is ready for PCR, Southern blotting and other molecular biology applications without any additional purification.
Chomczynski
P
P
Molecular Research Center, Cincinnati, OH, USA. piotr@mrcgene.com
Mackey
K
K
Drews
R
R
Wilfinger
W
W
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000302:isolation & purification*; D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D003902:Detergents; D004587:Electrophoresis, Agar Gel; D006146:Guanidines; D006801:Humans; D007202:Indicators and Reagents; D051379:Mice; D010944:Plants / Q000737:chemistry; D016133:Polymerase Chain Reaction; D051381:Rats; D013154:Spleen; D013623:Tail; D013861:Thiocyanates
D004279:DNA, Viral; D003902:Detergents; D006146:Guanidines; D007202:Indicators and Reagents; D013861:Thiocyanates; C054436:guanidine thiocyanate; D004247:DNA; D015247:Deoxyribonuclease HindIII