Establishment and characterization of a novel human cell line exhibiting both immunophenotypic markers of monocyte/macrophage and natural killer cell lineages from peripheral blood of a patient with atopic dermatitis.
Large amounts of homogeneous cells are not always available for in vitro studies of inflammatory skin disorders. Here we demonstrate that a novel cell line, termed YAA, has been established from peripheral blood mononuclear cells, which were separated by the Ficoll method, of a patient with atopic dermatitis. YAA cells were grown in suspension culture. The cytochemical staining showed a positive reaction for alpha-naphthyl butyrate esterase, which was completely inhibited by sodium fluoride, but a negative result for periodic acid-Schiff, peroxidase and alkaline phosphatase. A large population of YAA cells exhibited phenotype of CD33 and CD56 but neither of CD2 nor CD3. The phorbol ester-stimulated YAA cells produced a considerable amount of tumor necrosis factor-alpha. These findings suggest that YAA might be a monocytoid line with an additional phenotype specific for natural killer cells.
Hamamoto
Y
Y
Department of Dermatology, Yamaguchi University School of Medicine, Japan.
Nagai
K
K
Muto
M
M
Nakamura
K
K
Yasui
H
H
Asagami
C
C
D000328:Adult; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D019070:Cell Lineage; D002875:Chromosomes / Q000145:classification / Q000235:genetics; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology; D003876:Dermatitis, Atopic / Q000097:blood* / Q000276:immunology; D006651:Histocytochemistry; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology
D000954:Antigens, Surface; D016207:Cytokines; D010835:Phytohemagglutinins