The differentiation antigen Ly-6E.1 is expressed in mouse metastatic tumor cell lines.
We report the cloning of the mouse surface GPI-anchored Ly-6E.1 protein from a highly metastatic mouse adenocarcinoma cell line CSML-100 by differential display. The expression is specific for the metastatic cell line as the closely related, non-metastatic mouse adenocarcinoma cell line CSML-0 does not express Ly-6E.1. Northern blot analysis reveals expression in a number of mouse tumour cell lines, exclusively metastatic ones. To date, active Ly-6A/E has only been described in lymphoid cells. The correlation between Ly-6E.1 expression, and the ability to metastasize, is discussed.
Cohn
M A
MA
Danish Cancer Society, Department of Molecular Cancer Biology, Copenhagen. cohn@biobase.dk
Kramerov
D
D
Hulgaard
E F
EF
Lukanidin
E M
EM
D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology; D000595:Amino Acid Sequence; D000818:Animals; D000950:Antigens, Ly / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D000950:Antigens, Ly; D018076:DNA, Complementary; D017261:Glycosylphosphatidylinositols