Histidine ligand affinity chromatography.
The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of proteins, with relevant interaction mechanism are described. Practical protocols of sample quality, capacity and scaled up and scaled down operations are discussed. Possibilities of pyrogen removal from high value blood proteins and their simultaneous recovery in the pure form, using histidine immobilized sorbents are described.
Vijayalakshmi
M A
MA
Laboratoire d'Intéractions Moléculaires et de Technologie des Séparations, University of Technology at Compiègne, France.
D002846:Chromatography, Affinity; D006639:Histidine; D008024:Ligands
D008024:Ligands; D006639:Histidine