title: Spectrin localizations in the chicken bursa of Fabricius at different stages of development.
abstract: Before and after hatching, light microscopic and ultrastructural observations of spectrin were performed immunohistochemically in the chicken bursa of Fabricius. Before hatching, the frequency of spectrin-positive cells was very low. Among the spectrin-positive cells spectrin was mostly detected in patchy or diffuse form in the cytoplasm and rarely seen at surface membranes. Although cortical lymphocytes were spectrin-negative, numerous medullary lymphocytes were spectrin-positive after hatching. In the medullary spectrin-positive cells, staining intensity was uniform. Spectrins showed ultrastructural heterogeneity after hatching. Although a new type of spectrin localization associated with surface membranes was observed, this type of spectrin localization was not prominent. The increased number of spectrin-positive cells, uniform staining intensity and the localization of spectrin associated with surface membranes seem to coincide with B cell differentiation.
authors.affiliation: Department of Bacteriology, Saitama Medical School, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Moriya
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism / Q000648:ultrastructure; D002060:Bursa of Fabricius / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens / Q000502:physiology*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D013049:Spectrin / Q000378:metabolism*
chemical_list: D013049:Spectrin