Extracellular ATP-stimulated increase of cytosolic cAMP in HL-60 cells.
Extracellular ATP increases intracellular Ca2+ concentration ([Ca2+]i) through activation of P2U purinoceptors in HL-60 cells. We found that extracellular ATP also increased the intracellular cAMP level in undifferentiated HL-60 cells. ATP and the hydrolysis-resistant ATP analogue adenosine 5'-O-(3-thiotriphosphate) triggered cAMP production in a concentration-dependent manner. The effects of ATP analogues on the [Ca2+]i elevation was distinguished from their effects on cAMP generation. Reactive Blue 2, the antagonist of P2 purinoceptors, inhibited ATP-induced cAMP generation in a concentration-dependent manner without inhibiting Ca2+ mobilization. The results suggest that a distinct P2 purinoceptor, one different from P2U, is linked to adenylyl cyclase in HL-60 cells.
Choi
S Y
SY
Department of Life Science, Pohang University of Science and Technology, Republic of Korea.
Kim
K T
KT
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis*; D003600:Cytosol / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D018048:Receptors, Purinergic P2 / Q000502:physiology
D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D002118:Calcium