(+)cis-dioxolane stimulation of cytosolic calcium oscillations and phasic contractions of myometrial smooth muscle.
These studies sought to determine the intracellular mechanisms underlying muscarinic receptor stimulated phasic contractions of myometrial smooth muscle. Utilizing cytosolic calcium imaging studies with Fura-2 loaded muscle strips, along with in vitro isometric contraction studies, we have demonstrated that phasic myometrial contractions stimulated in response to (+)cis-dioxolane, a muscarinic selective acetylcholine agonist, are mediated by activation of the M3 receptor subtype resulting in stimulation of the phosphatidylinositol signaling pathway and the generation of cytosolic calcium oscillations.
Phillippe
M
M
Department of Obstetrics & Gynecology, University of Chicago, Illinois 60637, USA.
Basa
A
A
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004148:Dioxolanes / Q000494:pharmacology*; D005260:Female; D018721:Muscarinic Agonists / Q000494:pharmacology*; D009215:Myometrium / Q000378:metabolism*; D009355:Neomycin / Q000494:pharmacology; D010507:Periodicity; D010716:Phosphatidylinositols / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D014590:Uterine Contraction
D004148:Dioxolanes; D018721:Muscarinic Agonists; D010716:Phosphatidylinositols; D011976:Receptors, Muscarinic; D009355:Neomycin; D002118:Calcium; C010962:formal glycol