title: Changes in plasma arginine vasotocin (AVT) concentration and dorsal aortic blood pressure following AVT injection in the teleost Platichthys flesus.
abstract: Dorsal aortic blood pressure and plasma arginine vasotocin (AVT) concentrations have been assessed in free swimming, chronically cannulated flounder following AVT injection. Intraarterial AVT at doses of 4.76 x 10(-12) mol.kg-1 and greater caused a biphasic change in blood pressure (an initial fall followed by a sustained pressor response). Doses above 4.76 x 10(-12) mol.kg-1 were associated with plasma AVT concentrations (20 min after injection) 2-3 orders of magnitude greater than the physiological range and must be considered pharmacological. Injection of the lowest pressor AVT dose (4.76 x 10(-12) mol.kg-1), 20 min after injection, increased plasma AVT concentrations to 23.4 +/- 6.1 fmol x ml-1. This increase is close to plasma AVT concentrations recently reported in untreated fish; however, in the initial period after injection plasma levels were calculated to be considerably higher than the physiological range. These results confirm that AVT is pressor in flounder but suggest that the pressor response may occur only at circulating AVT levels above the normal physiological range. The biphasic response to AVT and the differing responses to mammalian V1 and V2 type receptor agonists in the current work suggests that AVT may contribute to regional blood flow distribution in teleosts rather than blood pressure regulation.
authors.affiliation: School of Biological Sciences, University of Manchester, United Kingdom.
authors.forename: J M
authors.initials: JM
authors.lastname: Warne
authors.forename: R J
authors.initials: RJ
authors.lastname: Balment
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology; D001794:Blood Pressure / Q000187:drug effects*; D003894:Deamino Arginine Vasopressin / Q000031:analogs & derivatives / Q000494:pharmacology; D005432:Flounder / Q000502:physiology*; D007700:Kinetics; D010121:Oxytocin / Q000031:analogs & derivatives / Q000494:pharmacology; D017483:Receptors, Vasopressin / Q000819:agonists; D014668:Vasotocin / Q000097:blood* / Q000494:pharmacology*
chemical_list: D017483:Receptors, Vasopressin; C034862:vasotocin receptor; C047063:oxytocin, Phe(2)-Orn(8)-; D010121:Oxytocin; C007223:vasopressin, 1-deamino-4-Val-8-Arg-; D003894:Deamino Arginine Vasopressin; D014668:Vasotocin