NMR evidence for a novel asparagine-linked oligosaccharide on cellobiohydrolase I from Trichoderma reesei RUTC 30.
The primary structure of a novel phosphate-containing oligosaccharide, isolated from T. reesei cellobiohydrolase I, was determined by NMR techniques. The new compound has the same structure as GlcMan7GlcNAc2, but it is extended by one alpha-mannopyranosyl unit (Man-P) through a phosphate link. Three different heteronuclear (31P-1H) NMR techniques were used to prove that the phosphate links the glycosidic site of Man-P with C-6 of unit Man-B. The presence of mannoses linked through a phosphate diester resembles glycosyl synthesis in yeast.
De Bruyn
A
A
Laboratory of Organic Chemistry, University of Ghent, Belgium.
Maras
M
M
Schraml
J
J
Herdewijn
P
P
Contreras
R
R
D001216:Asparagine / Q000032:analysis* / Q000737:chemistry; D002240:Carbohydrate Sequence; D002480:Cellulase / Q000737:chemistry*; D043366:Cellulose 1,4-beta-Cellobiosidase; D005656:Fungal Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D014242:Trichoderma / Q000201:enzymology*
D005656:Fungal Proteins; D009844:Oligosaccharides; D001216:Asparagine; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase