VE-cadherin antibody accelerates neutrophil recruitment in vivo.
Neutrophils enter sites of inflammation by crossing the endothelial lining of the blood vessel wall. VE-cadherin is an endothelial specific, homophilic adhesion molecule located at the lateral cell surface. We have generated a monoclonal antibody against mouse VE-cadherin which inhibits electrical resistance of endothelial cell monolayers in vitro as well as aggregation of VE-cadherin transfected cells. In vivo, this antibody was found to increase vascular permeability and to accelerate the entry of neutrophils into chemically inflamed mouse peritoneum. Thus, VE-cadherin is essential for the integrity of the endothelial barrier in vivo. Our data suggest that opening of VE-cadherin mediated endothelial cell contacts may be a relevant step during neutrophil extravasation.
Gotsch
U
U
Institute of Cell Biology, ZMBE, University of Münster, Germany.
Borges
E
E
Bosse
R
R
Böggemeyer
E
E
Simon
M
M
Mossmann
H
H
Vestweber
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D016466:CHO Cells; D015820:Cadherins / Q000235:genetics / Q000276:immunology*; D002199:Capillary Permeability / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D002449:Cell Aggregation / Q000276:immunology*; D006224:Cricetinae; D051379:Mice; D009504:Neutrophils / Q000166:cytology* / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D014162:Transfection; D014407:Tumor Cells, Cultured; D000099152:Cadherin 5
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015820:Cadherins; D000099152:Cadherin 5