The effect in vitro of high-density lipoprotein from healthy and infected humans on the oxidative metabolism of polymorphonuclear leukocytes.
We studied the effects in vitro of high-density lipoprotein from healthy (N-HDL) and from infected humans (AP-HDL) on the oxidative metabolism of human polymorphonuclear leukocytes (PMN). Products of the H2O2-MPO-halide system were monitored by luminol-enhanced chemiluminescence and superoxide anion formation was monitored by lucigenin-enhanced chemiluminescence during stimulation of human PMN with phorbol myristate acetate (PMA) or an opsonized stimulus (OS). The results showed that N-HDL and AP-HDL affect the oxidative metabolism of PMN in different ways. The possible role of this effect is discussed.
Furlaneto
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Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brasil.
Campa
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D000166:Acridines; D002273:Carcinogens / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007239:Infections / Q000276:immunology / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology* / Q000382:microbiology; D009195:Peroxidase / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects* / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000166:Acridines; D002273:Carcinogens; D008075:Lipoproteins, HDL; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol; D006861:Hydrogen Peroxide; D009195:Peroxidase; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate