title: Human sperm morphometry and smoking, caffeine, and alcohol consumption.
abstract: The purpose of this study is to determine whether sperm nuclear size, shape, and chromatin texture parameters are associated with lifestyle exposures including smoking, caffeine, and alcohol consumption. Eighty-six healthy male volunteers (ages 18-35), recruited through newspaper advertisements, provided a semen, blood, and urine sample and completed a questionnaire concerning demographic and lifestyle exposures. Sperm nuclear size, shape, and chromatin texture parameters were measured using computerized image analysis. Results indicated no associations between the sperm nuclear morphometric parameters and age, smoking, or alcohol consumption. There was weak evidence for an association with caffeine intake. In conclusion, the lifestyle factors smoking, caffeine intake, and alcohol consumption do not appear to significantly affect sperm nuclear size, shape, or chromatin texture in this study population.
authors.affiliation: Department of Epidemiology, University of North Carolina, Chapel Hill 27599-7400, USA.
authors.forename: M F
authors.initials: MF
authors.lastname: Vine
authors.forename: R W
authors.initials: RW
authors.lastname: Setzer
authors.forename: R B
authors.initials: RB
authors.lastname: Everson
authors.forename: A J
authors.initials: AJ
authors.lastname: Wyrobek
mesh_terms: D000328:Adult; D000434:Alcoholic Beverages / Q000009:adverse effects*; D002110:Caffeine / Q000009:adverse effects*; D002467:Cell Nucleus / Q000187:drug effects; D002843:Chromatin / Q000187:drug effects; D000431:Ethanol / Q000009:adverse effects; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007620:Karyometry; D008019:Life Style; D008297:Male; D012907:Smoking / Q000009:adverse effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D002110:Caffeine; D000431:Ethanol