Deoxycholic acid and SCFA-induced apoptosis in the human tumor cell-line HT-29 and possible mechanisms.
Short chain fatty acids (propionate and butyrate) and deoxycholic acid (DCA) are able to induce apoptosis in HT-29 colonic tumor cell line, but DCA induces a much higher level of apoptosis than butyrate and propionate. Mixtures of DCA with butyrate or propionate enhance the effect of the single components. Apoptosis is not affected by the PKC, PTK or de novo mRNA and protein synthesis inhibitors, so that the involvement of these enzymes and processes is ruled out. In contrast, DCA-induced apoptosis is directly related to [Ca2+]i concentration as demonstrated by the apoptosis inhibition caused by [Ca2+]i chelator BAPTA/AM.
Marchetti
C
C
Department of Cell and Molecular Biology, University of Perugia, Italy.
Migliorati
G
G
Moraca
R
R
Riccardi
C
C
Nicoletti
I
I
Fabiani
R
R
Mastrandrea
V
V
Morozzi
G
G
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002118:Calcium / Q000032:analysis; D002614:Chelating Agents / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000737:chemistry / Q000473:pathology*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D002087:Butyrates; D002614:Chelating Agents; D004791:Enzyme Inhibitors; D007529:Isoflavones; D011422:Propionates; D011500:Protein Synthesis Inhibitors; D003840:Deoxycholic Acid; D020148:Butyric Acid; D003609:Dactinomycin; D004533:Egtazic Acid; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D003513:Cycloheximide; D019833:Genistein; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium