title: Systemic osteopenia and mineralization defect in patients with ankylosing spondylitis.
abstract: OBJECTIVE
  Low bone mass, vertebral osteopenia, and fractures have been recognized in patients with ankylosing spondylitis (AS). However, there are few data about bone histology and histomorphometric changes in these patients. To shed light on bone alterations of these patients, we carried out a study including static and dynamic variables of bone of patients with AS, using iliac crest bone biopsy.
  
  
  METHODS
  16 white men with AS, mean age 34 +/- 3 years (15 to 55), mean duration of disease 11 +/- 2 years (6 months to 27) underwent bone biopsy for mineralized bone histology and evaluation of histomorphometric variables.
  
  
  RESULTS
  14 patients presented osteopenia, 10 mineralization defects, and 3 osteomalacia. Trabecular bone mass, trabecular wall thickness, trabecular plate, and wall thickness were significantly lower than the control values. Comparing bone formation variables we found that the relative osteoid volume and the thickness of osteoid were significantly greater than control values (p < 0.05); comparing bone resorption variables we found that the bone osteoclast interface and the eroded surface were similar to that obtained in male controls. Analyzing dynamic variables, we observed that mineral apposition rate and doubly labeled osteoid were significantly less than the control group (p < 0.05), and mineralization lag time was statistically greater than the control group (p < 0.01). There was positive correlation between the duration of disease with relative (r = 0.513, p < 0.05) and absolute osteoid volume (r = 0.590, p < 0.05). There was negative correlation between disease duration and eroded surface (r = -0.616, p < 0.01) and bone osteoclast interface (r = -0.481, p < 0.05). There was positive correlation between duration of disease and singly labeled trabeculae (r = 0.680, p < 0.01), duration of disease and singly labeled osteoid seam, and duration of disease and mineralization lag time (r = 0.439, p < 0.05).
  
  
  CONCLUSIONS
  Low bone mass in male patients with AS may also be related to mineralization defect. As bone resorption variables were normal in our patients, it is possible that the reduced bone mass seen in AS is due to a depression in bone formation rather than an increase in bone resorption.
authors.affiliation: Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brazil.
authors.forename: V L
authors.initials: VL
authors.lastname: Szejnfeld
authors.forename: M C
authors.initials: MC
authors.lastname: Monier-Faugere
authors.forename: B J
authors.initials: BJ
authors.lastname: Bognar
authors.forename: M B
authors.initials: MB
authors.lastname: Ferraz
authors.forename: H H
authors.initials: HH
authors.lastname: Malluche
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy; D015519:Bone Density; D001851:Bone Diseases, Metabolic / Q000209:etiology / Q000473:pathology*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology*; D002113:Calcification, Physiologic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011859:Radiography; D013167:Spondylitis, Ankylosing / Q000150:complications / Q000473:pathology*