Immobilized Lotus tetragonolobus agglutinin binds oligosaccharides containing the Le(x) determinant.
A defined set of oligosaccharides and glycopeptides containing alpha-linked fucose were used to examine the specificity of the immobilized fucose-binding lectin Lotus tetragonolobus agglutinin (LTA1), also known as lotus lectin. Glycans containing the Lewis x determinant (Le(x)) Gal beta 1-4[Fuc alpha 1-3]GlcNAc beta 1-3-R were significantly retarded in elution from high density LTA-Emphaze columns. The lectin also bound the fucosylated lacdiNAc trisaccharide GalNAc beta 1-4[Fuc alpha 1-3]GlcNAc. The lectin did not bind glycans containing either sialylLe(x) or VIM-2 determinants, nor did it bind the isomeric Le(x), Gal beta 1-3[Fuc alpha 1-4]GlcNAc-R. Although 2'-fucosyllactose Fuc alpha 1-2Gal beta 1-4Glc) was retarded in elution from the columns, larger glycans containing the H-antigen Fuc alpha 1-2Gal beta 1-3(4)GlcNAc-R interacted poorly with immobilized LTA. Our results demonstrate that immobilized LTA is effective in isolating glycans containing the Le(x) antigen and is useful in analyzing specific fucosylation of glycoconjugates.
Yan
L
L
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
Wilkins
P P
PP
Alvarez-Manilla
G
G
Do
S I
SI
Smith
D F
DF
Cummings
R D
RD
D000373:Agglutinins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D019556:COS Cells / Q000737:chemistry / Q000378:metabolism; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D005643:Fucose / Q000737:chemistry / Q000378:metabolism; D005647:Fucosyltransferases / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D037102:Lectins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D007983:Lewis Blood Group Antigens / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000276:immunology*; D009961:Orosomucoid / Q000737:chemistry / Q000378:metabolism; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000378:metabolism
D000373:Agglutinins; D006023:Glycoproteins; D037102:Lectins; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D009961:Orosomucoid; D037121:Plant Lectins; D011134:Polysaccharides; D011994:Recombinant Proteins; D000509:alpha-Fetoproteins; C027112:fucose-binding lectin; D005643:Fucose; D005647:Fucosyltransferases