title: Purification of the lipophosphoglycan from two Leishmania mexicana strains by lectin affinity chromatography.
abstract: Two lipophosphoglycans (LPG) from two Leishmania mexicana (Soberano and Yucatan) strains, isolated in Mexico, were purified by affinity chromatography with the Con A lectin. The LPG from each strain, with a 10 kDa molecular weight, possesses two fractions: one with high mannose concentrations and the other with a more heterogeneous saccharidic composition; the mannose-rich fraction and the heterogeneous fraction from the Yucatan strain show a single band of identity with rabbit IgG against promastigotes from L. mexicana Yucatan. Antigen recognition was abolished by treating the high mannose LPGs with alpha-mannosidase. The presence of non reductor alpha-mannose sequences, as determinant epitopes in L. mexicana Soberano and Yucatan strains, was determined by mass spectrometry analysis and enzymatic cleavage.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, México, D.F.
authors.forename: P
authors.initials: P
authors.lastname: Gorocica
authors.forename: A
authors.initials: A
authors.lastname: Monroy
authors.forename: B
authors.initials: B
authors.lastname: Rivas
authors.forename: S
authors.initials: S
authors.lastname: Estrada-Parra
authors.forename: Y
authors.initials: Y
authors.lastname: Leroy
authors.forename: R
authors.initials: R
authors.lastname: Chávez
authors.forename: E
authors.initials: E
authors.lastname: Zenteno
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel; D003208:Concanavalin A; D005260:Female; D006028:Glycosphingolipids / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007894:Leishmania mexicana / Q000737:chemistry*; D013058:Mass Spectrometry; D011817:Rabbits
chemical_list: D006028:Glycosphingolipids; C008290:lipophosphonoglycan; D003208:Concanavalin A