title: Morphology and steroidogenesis of cultured granulosa cells obtained from ovaries of women treated with ionizing radiation.
abstract: The object of the study was the morphology and steroidogenesis of cultured granulosa cells obtained from 6 women aged 28-39 years who, because of Ib cervix carcinoma, were treated with ionizing radiation and later underwent surgery. It was observed that the granulosa cells were viable, had strong proliferative ability, and formed a monolayer on day 2 of culture. Contrary to our expectations, these cells produced larger amounts of steroids in culture than the control cells harvested from normal ovaries in late follicular phase. It was also found that the cells treated with ionizing radiation responded to exogenous gonadotropins with higher production of progesterone and estradiol than the controls. It is concluded that the increase in metabolic activity by granulosa cells from ovaries which had been indirectly affected by ionizing radiation is manifested by the stimulating influence of radiation on steroidogenesis.
authors.affiliation: Department of Reproduction, Medical Academy, Poznań, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Skrzypczak
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000532:radiotherapy; D002478:Cells, Cultured / Q000378:metabolism / Q000528:radiation effects; D004958:Estradiol / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D010053:Ovary / Q000166:cytology*; D011374:Progesterone / Q000096:biosynthesis / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*; D013739:Testosterone / Q000096:biosynthesis / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000532:radiotherapy
chemical_list: D013256:Steroids; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol