Application of triphenyltetrazolium chloride in microbial limit test in pharmaceuticals and cosmetics. Multiple- tube method for yeasts detection.
The triphenyltetrazolium chloride (TTC) was studied as a means to detect yeasts growth in liquid medium, aiming its application to microbial limit test using Multiple-tube method, for testing pharmaceuticals or cosmetics samples with opacity. The results from most probable number (MPN) using TTC and sub-culture techniques obtained from the tests of simulated samples, spiked with Candida albicans and Sacharomyces cerevisiae, showed to be equivalents.
Ohara
M T
MT
Departamento de Farmácia, Faculdade de Ciĕncias Farmacĕuticas, Universidade de São Paulo, Brazil.
Saito
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D002176:Candida albicans / Q000187:drug effects; D003358:Cosmetics / Q000592:standards*; D004340:Drug Contamination / Q000517:prevention & control*; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013778:Tetrazolium Salts; D015003:Yeasts / Q000187:drug effects*
D003358:Cosmetics; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium