Characterization of monoglucuronides of vitamin D2 and 25-hydroxyvitamin D2 in rat bile using high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.
The characterization of vitamin D2 3-glucuronide, 25-hydroxyvitamin D2 3-glucuronide and 25-hydroxyvitamin D2 25-glucuronide, biliary metabolites obtained from rats dosed with vitamin D2 and 25-hydroxyvitamin D2 per os, was carried out using HPLC-atmospheric pressure chemical ionization (APCI)-MS. The glucuronide obtained from bile specimens was identified by comparison of its chromatographic behaviour with an authentic sample using HPLC-APCI-MS operating in the negative-ion mode. Methylation of the respective fraction with diazomethane gave the methyl ester, which was also confirmed by HPLC-APCI-MS operating in the positive-ion mode. The (M-H)- and (M + NH4)+ ions were monitored in the selected-ion monitoring mode.
Shimada
K
K
Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Mitamura
K
K
Nakatani
I
I
D015652:25-Hydroxyvitamin D 2 / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D001646:Bile / Q000737:chemistry*; D002853:Chromatography, Liquid; D004872:Ergocalciferols / Q000032:analysis*; D005965:Glucuronates / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar
C106028:25-hydroxyvitamin D2 3-glucuronide; D004872:Ergocalciferols; D005965:Glucuronates; D015652:25-Hydroxyvitamin D 2; C036286:vitamin D2 glucosiduronate; C028862:25-hydroxyvitamin D2-25-glucuronide