Histochemical study of the influence of transplanted teeth with periodontal ligament of the binding of peanut agglutinin in rat dorsal skin.
Our previous study showed that the expression of carbohydrate residues in junctional epithelium (JE) after resection is closely related to attachment and stratification along the dental root surface. However, the influence of the periodontal ligament on carbohydrate expression has still not been clarified. In this study we examined the relationship between the presence of periodontal ligament and the expression of carbohydrate residues on epithelium regenerating along root surfaces. We transplanted extracted rat molars with or without periodontal ligament tissue, repeatedly frozen and thawed teeth with ligament and demineralized teeth without ligament into the dorsal skin of rats. After 2, 3, 5, 7, 10, 14 or 21 d, dorsal cutaneous tissues containing transplanted teeth were resected, fixed, decalcified and embedded in paraffin. Serial sections were stained histochemically with the HRP-conjugated lectin, peanut agglutinin (PNA) to observe the expression of carbohydrate residues in regenerating epithelium. Histochemical observation revealed that lectin binding reactions were changed from the characteristics of skin to those of JE when the regenerating epithelium was attached and stratified along the tooth with unfrozen or frozen tissue. These results suggested that the structural formation and expression of PNA in regenerating epithelium around the root surface were influenced by not only the tooth but also by the periodontal ligament.
Abe
Y
Y
Department of Periodontology, Nagasaki University School of Dentistry, Japan.
Hara
Y
Y
Kato
I
I
D000818:Animals; D010367:Arachis; D050260:Carbohydrate Metabolism; D002448:Cell Adhesion; D004396:Coloring Agents; D003648:Decalcification Technique; D062109:Dermatologic Surgical Procedures; D004846:Epithelial Attachment / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005500:Follow-Up Studies; D005615:Freezing; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism*; D008297:Male; D016612:Paraffin Embedding; D019887:Peanut Agglutinin; D010513:Periodontal Ligament / Q000378:metabolism / Q000637:transplantation*; D037121:Plant Lectins; D051381:Rats; D011917:Rats, Inbred Lew; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012038:Regeneration; D012867:Skin / Q000378:metabolism*; D016707:Tissue Fixation; D014070:Tooth / Q000378:metabolism / Q000637:transplantation*; D014092:Tooth Root / Q000473:pathology
D004396:Coloring Agents; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011956:Receptors, Cell Surface