Gelatinase activity and the occurrence of cerebral aneurysms.
OBJECTIVE
Cerebral aneurysms are associated with decreased arterial collagen content; however, whether this deficiency results from impaired collagen synthesis or enhanced collagen degradation is unknown. This study tested the hypothesis that enhanced collagen degradation, not impaired collagen synthesis, is associated with the occurrence of cerebral aneurysms.


METHODS
Cultured skin fibroblasts and serum samples were studied in patients with angiographic evidence of aneurysm (n = 31) and control subjects (n = 14). Transcription of the type III collagen gene was assessed with the use of Northern blots prepared from RNA harvested from confluent cultured fibroblasts. Translation of type III collagen was assessed by Western blot analysis of proteins produced by cultured skin fibroblasts. Collagen metabolism was assessed by radioimmunoassay for type I (PICP) and type III (PIIINP) procollagen peptides in conditioned tissue culture media and serum. We assessed collagen degradation in serum and conditioned tissue culture media by evaluating gelatinase activity using quantitative zymography.


RESULTS
Type III collagen synthesis was the same in aneurysm and control patients. Neither the molecular weight nor the relative amount of type III collagen mRNA differed between aneurysm and control patient fibroblasts. Western blot analysis revealed no difference in the relative amount or molecular weight of procollagen III synthesized by aneurysm and control cells. Aneurysm patients had a threefold increase in native serum gelatinase activity compared with control subjects (P = .004). This increase occurred along with serum evidence of increased collagen metabolism. Serum levels of PICP (P = .03) and PIIINP (P = .02) were decreased in aneurysm patients. Elevated serum gelatinase activity and altered collagen metabolism could not be explained by enhanced secretion of gelatinase by cultured fibroblasts or altered net collagen synthesis by fibroblasts. High serum gelatinase activity was more common in men than in women (P = .04).


CONCLUSIONS
These findings are consistent with the hypothesis that accelerated enzymatic degradation of collagens and other structural proteins compromises the mechanical integrity of the cerebral vessel wall and leads to conditions that favor aneurysm formation.
Chyatte
D
D
Cerebrovascular Research Laboratory, Cleveland Clinic Foundation, Ohio 44195, USA. chyattd@cesmtp.ccf.org
Lewis
I
I
D000368:Aged; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D018093:Gelatinases / Q000097:blood / Q000378:metabolism*; D006801:Humans; D002532:Intracranial Aneurysm / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012016:Reference Values; D012867:Skin / Q000201:enzymology / Q000473:pathology
D017077:Culture Media, Conditioned; D010446:Peptide Fragments; D011347:Procollagen; D012333:RNA, Messenger; D003094:Collagen; D018093:Gelatinases