title: A serine alkaline protease from the fungus Conidiobolus coronatus with a distinctly different structure than the serine protease subtilisin Carlsberg.
abstract: In view of the functional similarities between subtilisin Carlsberg and the alkaline protease from Conidiobolus coronatus, the biochemical and structural properties of the two enzymes were compared. In spite of their similar biochemical properties, e.g., pH optima, heat stability, molecular mass, pI, esterase activity, and inhibition by diisopropyl fluorophosphate and phenylmethlysulfonylfluoride, the proteases were structurally dissimilar as revealed by (1) their amino acid compositions, (2) their inhibition by subtilisin inhibitor, (3) their immunological response to specific anti-Conidiobolus protease antibody, and (4) their tryptic peptide maps. Our results demonstrate that although they are functionally analogous, the Conidiobolus protease is structurally distinct from subtilisin Carlsberg. The Conidiobolus protease was also different from other bacterial and animal proteases (e.g. pronase, protease K, trypsin, and chymotrypsin) as evidenced by their lack of response to anti-Conidiobolus protease antibody in double diffusion and in neutralization assays. The Conidiobolus serine protease fails to obey the general rule that proteins with similar functions have similar primary sequences and, thus, are evolutionarily related. Our results strengthen the concept of convergent evolution for serine proteases and provide basis for research in evolutionary relationships among fungal, bacterial, and animal proteases.
authors.affiliation: Biochemical Sciences Division, National Chemical Laboratory, Pune-411008, India.
authors.forename: S
authors.initials: S
authors.lastname: Phadtare
authors.forename: M
authors.initials: M
authors.lastname: Rao
authors.forename: V
authors.initials: V
authors.lastname: Deshpande
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000908:Antibodies, Fungal / Q000276:immunology; D002918:Chymotrypsin / Q000032:analysis; D019286:Endopeptidase K / Q000032:analysis; D004773:Entomophthora / Q000201:enzymology*; D005459:Fluorides / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009500:Neutralization Tests; D010449:Peptide Mapping; D010710:Phosphates / Q000494:pharmacology; D010802:Phylogeny; D011402:Pronase / Q000032:analysis; D012697:Serine Endopeptidases / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D015842:Serine Proteinase Inhibitors / Q000378:metabolism; D013381:Subtilisins / Q000037:antagonists & inhibitors / Q000737:chemistry; D014357:Trypsin / Q000032:analysis
chemical_list: D000596:Amino Acids; D000908:Antibodies, Fungal; D010710:Phosphates; D015842:Serine Proteinase Inhibitors; C012980:fluorophosphate; D012697:Serine Endopeptidases; D013381:Subtilisins; D002918:Chymotrypsin; D014357:Trypsin; D019286:Endopeptidase K; D011402:Pronase; D005459:Fluorides