title: Occurrence of paralytic shellfish poison in Bangladeshi freshwater puffers.
abstract: Two species of freshwater puffer fish, Tetraodon cutcutia and Chelonodon patoca, collected from several locations in Bangladesh, showed lethal potency in mice ranging from 2.0 to 40.0 MU/g tissue as paralytic shellfish poison. In both species, toxicity of the skin was generally higher than the other tissues examined (muscle, liver and ovary). Water-soluble toxins from T. cutcutia were partially purified by activated charcoal treatment followed by column chromatographies using Bio-Gel P-2 and Bio-Rex 70. Analyses by cellulose acetate membrane electrophoresis and high-performance liquid chromatography with fluorometric detection demonstrated that the toxins were composed of saxitoxin, decarbamoylsaxitoxin, gonyautoxins 2 and 3, decarbamoylgonyautoxins 2 and 3, and three unidentified components which are possibly related to paralytic shellfish poison.
authors.affiliation: Laboratory of Marine Botany and Environmental Science, Faculty of Fisheries, Kagoshima University, Japan.
authors.forename: L
authors.initials: L
authors.lastname: Zaman
authors.forename: O
authors.initials: O
authors.lastname: Arakawa
authors.forename: A
authors.initials: A
authors.lastname: Shimosu
authors.forename: Y
authors.initials: Y
authors.lastname: Onoue
mesh_terms: D000818:Animals; D001459:Bangladesh; D002851:Chromatography, High Pressure Liquid; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D005397:Fish Venoms / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D012015:Reference Standards; D012530:Saxitoxin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity*
chemical_list: D005397:Fish Venoms; D012530:Saxitoxin; C034903:decarbamylsaxitoxin; C013007:gonyautoxins