Arrangement of the COOH-terminal and NH2-terminal domains of caldesmon bound to actin.
Smooth muscle caldesmon is a single polypeptide chain with its NH2- and COOH-terminal domains separated by a long alpha-helix. Caldesmon was labeled at either Cys-153 in the NH2 domain or Cys-580 in the COOH domain with a variety of fluorescence probes. Fluorescence intensity, peak position, and polarization of probes on Cys-580 were very sensitive to the binding to actin (with or without tropomyosin), whereas for probes on Cys-153, there was a lack of response, in reconstituted or native actin thin filaments. From fluorescence resonance energy transfer from donor labels on either caldesmon cysteine to acceptor labels on Cys-374 of actin, the distance between the donor and acceptor was estimated to be 27 A for the donor at Cys-580 and 65-80 A for the donor at Cys-153. These findings were the same for caldesmon prepared with or without heat treatment and with striated or smooth muscle actin. These results, together with previous knowledge that COOH-terminal fragments of caldesmon bind to actin whereas NH2-terminal fragments do not, indicate that, while the COOH domain of caldesmon is bound to actin, the NH2 domain is largely dissociated. Fluorescence quenching studies showed that actin binding to caldesmon greatly decreased the accessibility of probes at caldesmon Cys-580 to the quencher, whereas for probes at Cys-153, actin afforded much less, but significant, protection from quenching. Consequently, it appears that, although the NH2 domain is mostly dissociated, it spends some time in the vicinity of actin, through either a weak interaction with actin or collisions with actin and/or because of restricted flexibility which constrains the NH2 domain to be close to the actin filament. Since the NH2 domain of caldesmon binds to the neck region of myosin, a dissociated NH2 domain may account for caldesmon's ability to link myosin and actin filaments.
Graceffa
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Muscle Research Group, Boston Biomedical Research Institute, Massachusetts 02114, USA. graceffa@bbri.harvard.edu
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D007700:Kinetics; D008301:Maleimides / Q000378:metabolism; D009130:Muscle, Smooth / Q000737:chemistry; D009282:Naphthalenesulfonates / Q000378:metabolism; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013439:Sulfhydryl Reagents / Q000378:metabolism; D014335:Tropomyosin / Q000378:metabolism
D000199:Actins; D002148:Calmodulin-Binding Proteins; D003374:Coumarins; D005456:Fluorescent Dyes; D008301:Maleimides; D009282:Naphthalenesulfonates; D013439:Sulfhydryl Reagents; D014335:Tropomyosin; C006421:1,5-I-AEDANS; C038518:acrylodan; D015081:2-Naphthylamine; D003545:Cysteine