cDNA cloning of eel (Anguilla anguilla) somatolactin.
The nucleotide sequence of the cDNA coding for eel somatolactin (SL), a pituitary hormone belonging to the growth hormone (GH)/prolactin (PRL) family, has been determined. The full-length eel SL cDNA (1213 bp) encodes a protein of 229 amino acids (aa), with a putative signal peptide of 24 aa and a mature protein of 204 aa. Eel SL contains seven Cys residues found to be characteristic of SLs, and two potential N-glycosylation sites. Significant sequence homology between eel and seven other fish SLs (42% aa identity, 63% aa similarity) reveal SL to be highly conserved. A higher sequence identity of SL to GH than PRL is suggested by the comparison of these hormones in eel and chum salmon.
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Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex, UK.
Todd
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Rand-Weaver
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D000595:Amino Acid Sequence; D000811:Anguilla / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D029941:Fish Proteins; D005399:Fishes / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics; D008969:Molecular Sequence Data; D010907:Pituitary Hormones / Q000235:genetics*; D011388:Prolactin / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; C064448:somatolactin protein, fish; D011388:Prolactin; D013006:Growth Hormone