Identification of a new antibody population directed against a desmosomal plaque antigen in pemphigus vulgaris and pemphigus foliaceus.
Pemphigus vulgaris and pemphigus foliaceus are characterized by autoantibodies directed against transmembrane glycoproteins of desmosomes. F12, a human monoclonal autoantibody that binds to the desmosomal plaque, recognizes a 180-190-kDa doublet when immunoblotted against bovine tongue epithelium. Because F12 was derived from the peripheral blood lymphocytes of a patient with pemphigus vulgaris, we looked for the presence of anti-180-190-kDa antibodies in pemphigus vulgaris and pemphigus foliaceus serum. By immunoblot analysis, a third of the pemphigus serum contained anti-180-190-kDa antibodies that belonged to IgG subclass 1 or 3, unlike those that recognized desmogleins 1 and 3 (IgG4). By immunoelectron microscopy analysis on human oral mucosa and human skin with mAb to human IgG3, pemphigus serum containing anti-180-190 kDa antibodies recognized desmosomal plaques. The presence of antibodies with F12 properties in pemphigus serum was further demonstrated by a rabbit anti-F12 idiotype antiserum that allowed detection of F12 idiotype in serum with anti-180-190-kDa antibodies. These results indicate that some pemphigus vulgaris and pemphigus foliaceus serums contain antibodies that react with both intra- and extracellular structures of desmosomes and further demonstrate the heterogeneity of the autoimmune response in both types of pemphigus.
Joly
P
P
Groupe de Recherche en Immunopathologie (Institut Fedératif de Recherche Multidisciplinaire sur les Peptides, IFR 23), Faculte de Medecine et de Pharmacie, Center Hospitalier et Universitaire Charles Nicolle, Rouen, France.
Gilbert
D
D
Thomine
E
E
Zitouni
M
M
Ghohestani
R
R
Delpech
A
A
Lauret
P
P
Tron
F
F
D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001324:Autoantigens; D015820:Cadherins / Q000276:immunology*; D051183:Desmoglein 1; D051184:Desmoglein 3; D003896:Desmosomes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007158:Immunologic Techniques; D016253:Microscopy, Immunoelectron; D010392:Pemphigus / Q000097:blood / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001324:Autoantigens; D015820:Cadherins; C071894:DSG3 protein, human; D051183:Desmoglein 1; D051184:Desmoglein 3