title: Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways.
abstract: Pex14p, an S. cerevisiae peroxin, is attached to the outer face of the peroxisomal membrane and is a component of the protein import machinery. Pex14p interacts with both the PTS1 and PTS2 receptors. It is the only known peroxisomal membrane protein that binds the PTS2 receptor and might thus mediate the membrane docking event of PTS2-dependent protein import. These results suggest that the two import pathways overlap and, furthermore, that Pex14p represents the point of convergence. Pex14p also interacts with two other membrane-bound peroxins including Pex13p, another binding protein for the PTS1 receptor. The data presented here are consistent with the idea of a common translocation machinery for both PTS-dependent protein import pathways in the peroxisomal membrane.
authors.affiliation: Abteilung für Zellbiochemie, Ruhr-Universität Bochum, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Albertini
authors.forename: P
authors.initials: P
authors.lastname: Rehling
authors.forename: R
authors.initials: R
authors.lastname: Erdmann
authors.forename: W
authors.initials: W
authors.lastname: Girzalsky
authors.forename: J A
authors.initials: JA
authors.lastname: Kiel
authors.forename: M
authors.initials: M
authors.lastname: Veenhuis
authors.forename: W H
authors.initials: WH
authors.lastname: Kunau
mesh_terms: D001692:Biological Transport / Q000502:physiology; D002352:Carrier Proteins; D003593:Cytoplasm / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D026901:Membrane Transport Proteins; D008830:Microbodies / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000502:physiology; D000074401:Peroxins; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D011485:Protein Binding / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C499195:PEX14 protein, S cerevisiae; D000074401:Peroxins; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D018160:Receptors, Cytoplasmic and Nuclear; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins