The improved PAS-ELISA method for the diagnosis of the pathogen for banana mosaic heart rot disease.
As a fast and practical immunological test, ELISA has been widely applied both in research and diagnosis of plant viruses [1]. In its research utility, PAS-ELISA has been proven as an effective measure to obtain CMV-free micropropagated plantlets. In large-scale micropropagation of banana plantlets, we have been using it in our Quality Control Department to prepare CMV-free and/or TMV-free germplasm. From large-scale testing, we have found that component(s) in banana tissue can cause a nonspecific reaction which severely interferes with the diagnostic results, making the average optical density (OD490nm) value a much higher false positive, leading to an abnormally high percentage loss of the germplasm prepared. The procedure for preparation of the antigen extract from banana tissue has been modified to eliminate the false positive.
Huang
M
M
Shilong Agristar Dexing Aseptic Cultured Plant Co. Ltd. Dongwan, China.
Liao
Z
Z
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005638:Fruit / Q000821:virology*; D009029:Mosaic Viruses / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology*