title: Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination.
abstract: The detailed genomic organization of a gene-dense region at human chromosome 12p13, spanning 223 kb of contiguous sequence, was determined. This region is composed of 20 genes and several other expressed sequences. Experimental tools including RT-PCR and cDNA sequencing, combined with gene prediction programs, were utilized in the analysis of the sequence. Various computer software programs were employed for sequence similarity searches and functional predictions. The high number of genes with diverse functions and complex transcriptional patterns make this region ideal for addressing challenges of gene discovery and genomic characterization amenable to large-scale sequence analysis.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA. ma029926@bcm.tmc.edu
authors.forename: M A
authors.initials: MA
authors.lastname: Ansari-Lari
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
authors.forename: D M
authors.initials: DM
authors.lastname: Muzny
authors.forename: W
authors.initials: W
authors.lastname: Lee
authors.forename: R A
authors.initials: RA
authors.lastname: Gibbs
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D015870:Gene Expression / Q000235:genetics; D015698:Genomic Library; D006019:Glycolysis / Q000235:genetics; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D017422:Sequence Analysis, DNA / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics; D012984:Software
chemical_list: D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C095278:atrophin-1; D012194:RNA-Directed DNA Polymerase