Hemoperfusion with alpha-amanitin: an in vitro study.
The authors carried out sixteen hemoperfusions with alpha-amanitin in vitro in a closed system using active charcoal, Amberlite XAD-2 and Amberlite XAD-4 in hemoperfusion capsules. As a perfusion solution 4 liters of 0.9% NaCl solution was used. The alpha-amanitin concentration in the solution was 8.3 +/- 0.36 mg/L. Individual hemoperfusion lasted 5 hours. Two hundred and forty minutes of Amberlite XAD-2 hemoperfusion led to the zero values of alpha-amanitin concentration in 0.9% NaCl solution. When using active charcoal the adsorption capacity of the hemoperfusion capsule was already exhausted at 120 min. The results gathered suggest that the most effective alpha-amanitin hemoperfusion in vitro was obtained with Amberlite XAD-2 and the least effective with active charcoal. The authors recommend the use of hemoperfusion with Amberlite XAD-2 for acute intoxication with Amanita phalloides in humans up to 24-36 hours after poisoning.
Mydlík
M
M
4th Medical Clinic, Medical School, University of P.J. Safárik, Kosice, Slovak Republic.
Derzsiová
K
K
Klán
J
J
Zíma
T
T
D000327:Adsorption; D000546:Amanitins / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D002606:Charcoal / Q000378:metabolism; D015314:Dialysis Solutions / Q000592:standards; D006464:Hemoperfusion; D006801:Humans; D066298:In Vitro Techniques; D009145:Mushroom Poisoning / Q000628:therapy; D010316:Particle Size; D012117:Resins, Synthetic / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry / Q000378:metabolism
D000546:Amanitins; D015314:Dialysis Solutions; D012117:Resins, Synthetic; D002606:Charcoal; D012965:Sodium Chloride; C046797:amberlite