title: Binding of microtubules to transitional elements in oligodendrocytes of the myelin mutant taiep rat.
abstract: The presence of microtubules physically bound to smooth endoplasmic reticulum profiles of oligodendrocytes constitutes the most conspicuous feature observed in the myelin mutant taiep rat. The endoplasmic reticulum membranes associated with microtubules were morphologically characterized as transitional elements that constitute the intermediate compartment according to their topographic location close to the cis-Golgi apparatus. The development of this surprising microtubular defect appears to be associated with the early events of myelination. Transitional elements associated with microtubules operate in protein transport from endoplasmic reticulum to cis-Golgi. This microtubular defect could explain the dysmyelination and neurologic alterations observed in taiep rats. Moreover, these findings allow us to propose that there is a blockage of protein traffic at the intermediate compartment of taiep oligodendrocytes, a situation that could explain the hypomyelinated axons observed in this myelin mutant. The binding of microtubules to membranous organelles promotes the stabilization of microtubules, a feature that has important implications regarding its accumulation within the cytoplasm of oligodendrocytes during the temporal evolution of this neurologic disorder. The taiep rat is a myelin mutant with a long survival and could be a useful model for understanding dysmyelinating diseases in which the intracellular transport of myelin components is affected.
authors.affiliation: Facultad de Ciencias, Universidad de Valparaíso, Chile.
authors.forename: E
authors.initials: E
authors.lastname: Couve
authors.forename: J F
authors.initials: JF
authors.lastname: Cabello
authors.forename: J
authors.initials: J
authors.lastname: Krsulovic
authors.forename: M
authors.initials: M
authors.lastname: Roncagliolo
mesh_terms: D000818:Animals; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D018870:Endoplasmic Reticulum, Rough / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000648:ultrastructure*; D009836:Oligodendroglia / Q000648:ultrastructure*; D009900:Optic Nerve / Q000648:ultrastructure*; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012270:Ribosomes / Q000648:ultrastructure