The effect of a high-protein diet on cystathionine beta-synthase activity and its transcript levels in rat liver.
Hepatic cystathionine beta-synthase activity was increased in rats fed a high-protein diet for three or five days as compared to rats given laboratory chow (day 0). In the low-protein diet group, the enzyme activity decreased after both feeding periods. Similar changes in the cystathionine beta-synthase mRNA levels were observed in the rats fed these diets. The changes in cystathionine beta-synthase activity caused by alteration of the dietary protein content were mainly due to changes in the mRNA levels.
Yamamoto
N
N
Fukuokajogakuin Junior College, Fukuoka, Japan.
Tanaka
T
T
Noguchi
T
T
D000818:Animals; D015152:Blotting, Northern; D001835:Body Weight; D003541:Cystathionine beta-Synthase / Q000235:genetics / Q000378:metabolism*; D004044:Dietary Proteins / Q000008:administration & dosage*; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D004044:Dietary Proteins; D012333:RNA, Messenger; D003541:Cystathionine beta-Synthase