Can trifluoperazine protect mitochondria against reactive oxygen species-induced damage?
Trifluoperazine (TFP) (35 microM) prevents mitochondrial transmembrane potential (delta psi) collapse and swelling induced by 10 microM Ca2+ plus oxyradicals generated from delta-aminolevulinic acid autoxidation. In contrast with EGTA, TFP cannot restore the totally collapsed delta psi. So, TFP might not remove Ca2+ from its 'harmful site', but could impair the ROS-driven cross-linking between membrane-SH proteins. Our data are correlated with the protective uses of TFP against oxidative processes promoted by oxyradicals plus Ca2+.
Pereira
R de D
Rde D
Department of Biochemistry, Universidade Estadual Paulista (UNESP), Araraquara, São Paulo, Brazil.
Hermes-Lima
M
M
D000622:Aminolevulinic Acid / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000502:physiology*; D014268:Trifluoperazine / Q000494:pharmacology*
D017382:Reactive Oxygen Species; D014268:Trifluoperazine; D000622:Aminolevulinic Acid; D002118:Calcium