Methods for the detection and enumeration of Alicyclobacillus acidoterrestris and investigation of growth and production of taint in fruit juice and fruit juice-containing drinks.
Methods for the detection of Alicyclobacillus acidoterrestris at a level of 1 cell per 100 ml and enumeration with a sensitivity of 5 cells ml-1 were developed. Spores of A. acidoterrestris survived pasteurization and outgrew and multiplied at a similar rate to vegetative cells in both orange juice and apple juice. Alicyclobacillus acidoterrestris grew readily in orange juice, apple juice and a non-carbonated fruit juice-containing drink at temperatures of 25-44 degrees C producing a taint and elevated levels (1-100 ppb) of guaiacol. Isolates of A. acidoterrestris can be identified using the DuPont RiboPrinter. It was isolated from apple drinks, apple juice concentrate and freshly squeezed orange juice.
Pettipher
G L
GL
Reading Scientific Services Ltd, Lord Zuckerman Research Centre, Whiteknights, UK.
Osmundson
M E
ME
Murphy
J M
JM
D001406:Bacillaceae / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D002957:Citrus / Q000382:microbiology; D015169:Colony Count, Microbial; D005516:Food Microbiology; D005638:Fruit / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry; D006139:Guaiacol / Q000378:metabolism; D006358:Hot Temperature; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000254:growth & development
D006139:Guaiacol