Species-specific beta-N-acetylgalactosaminylation of serum IgG proteins.
Lectin blot analysis of bovine, goat, human, rabbit and mouse serum immunoglobulin G (IgG) samples revealed that Wisteria floribunda agglutinin (WFA) binds to the heavy chains of bovine, goat and human serum IgG proteins but not those of the rabbit and mouse proteins. WFA-positive light chain bands were also detected in bovine, goat and human serum IgG samples only after the filters were treated with Arthrobacter ureafaciens sialidase. The WFA-binding to these IgG proteins was abolished by treatment of the filter with sialidase and then beta-N-acetylhexosaminidase or N-glycanase prior to incubation with the lectin. WFA-agarose column chromatography of the oligosaccharides released by hydrazinolysis from the IgG samples followed by reduction with NaB3H4 revealed that 0.15, 0.09 and 0.07% of the total oligosaccharides from bovine, goat and human serum IgG samples bind to the column, respectively. Partial characterization of WFA-positive bovine IgG oligosaccharides by Bio-Gel P-4 column chromatography suggested that the major oligosaccharide is of non-fucosylated biantennary complex-type. These results indicate that beta-N-acetylgalactosaminylation occurs to N-linked sugar chains of heavy and light chains of IgG proteins in a species-specific manner.
Aoki
N
N
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Japan.
Matsuda
T
T
Sakiyama
T
T
Iwatsuki
K
K
Furukawa
K
K
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D006041:Goats; D006596:Hexosaminidases / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D037102:Lectins; D051379:Mice; D037121:Plant Lectins; D011817:Rabbits; D034781:Receptors, N-Acetylglucosamine; D013045:Species Specificity; D048828:beta-N-Acetyl-Galactosaminidase
D007074:Immunoglobulin G; D037102:Lectins; D037121:Plant Lectins; D034781:Receptors, N-Acetylglucosamine; C040129:wisteria lectin; D006596:Hexosaminidases; D048828:beta-N-Acetyl-Galactosaminidase