A murine model of pulmonary damage induced by lipopolysaccharide via intranasal instillation.
This study examines the intranasal instillation of lipopolysaccharide (LPS) into BALB/c mice causing acute pulmonary damage, due to neutrophil infiltration and sepsis. A dose response with LPS showed that an intranasal instillation of 167 microg/ml (10 microg/mouse) caused acute lung injury within 2-4 h and reached maximal damage at 24-48 h. We found the method of LPS administration for induction of acute pulmonary damage to be crucial. After 24 h post-LPS injection, a comparison showed a substantial increase in pulmonary damage with intranasal instillation of LPS. As for intravenous injection, it showed a baseline effect. This study indicates that LPS administered intranasally causes acute pulmonary damage, whereas with intravenous and intraperitoneal endotoxin administration a tissue-specific or similar degree of pulmonary injury may not develop.
Szarka
R J
RJ
Alberta Research Council, Carbohydrate Chemistry, Edmonton, Canada.
Wang
N
N
Gordon
L
L
Nation
P N
PN
Smith
R H
RH
D000208:Acute Disease; D000281:Administration, Intranasal; D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004927:Escherichia coli Infections / Q000139:chemically induced / Q000473:pathology*; D005260:Female; D007275:Injections, Intravenous; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000633:toxicity*; D008171:Lung Diseases / Q000139:chemically induced / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011552:Pseudomonas Infections / Q000139:chemically induced / Q000473:pathology*
D008070:Lipopolysaccharides