Cyclic electron transfer in Heliobacillus mobilis involving a menaquinol-oxidizing cytochrome bc complex and an RCI-type reaction center.
Flash-induced absorption changes arising from b-type hemes were studied on whole cells of Heliobacillus mobilis under physiological and redox-controlled conditions. The sensitivity of the monitored redox changes to inhibitors of cytochrome bc complexes and the redox potential dependence of reduction and oxidation reactions of cytochrome b-hemes demonstrate that the respective b-hemes are part of a cytochrome bc complex. Both the half-time and the extent of flash-induced reduction of cytochrome b titrated with apparent potentials of about -60 and -50 mV (both n = 2), respectively, i.e., close to the Em,7 value of the menaquinone (MK) pool, indicating a collisional interaction between menaquinol and the Qo site of the cytochrome bc complex. At strongly reducing ambient potentials (< -150 mV), a net flash-induced oxidation of b-hemes was observed in agreement with the Em,7 values of the individual hemes of -90 mV (b(h)) and -190 mV (b(l)) determined in equilibrium redox titrations on membrane fragments. From the extent of photooxidized b- and c-type hemes as well as P798+, a stoichiometry of 0.6-0.75 cytochrome bc complexes per photosynthetic reaction center was estimated. The kinetic behavior and also the energy profiles for Q-cycle turnover of the heliobacterial complex are compared to those of cytochrome bc1 complexes from purple bacteria and of cytochrome b6f complexes from chloroplasts.
Kramer
D M
DM
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340, USA. dkramer@wsu.edu
Schoepp
B
B
Liebl
U
U
Nitschke
W
W
D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D003573:Cytochrome b Group / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D004563:Electrochemistry; D004579:Electron Transport; D007700:Kinetics; D008027:Light; D008565:Membrane Proteins / Q000378:metabolism; D009284:Naphthols / Q000378:metabolism*; D010084:Oxidation-Reduction; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism; D013053:Spectrophotometry; D013729:Terpenes / Q000378:metabolism*; D014812:Vitamin K / Q000378:metabolism*
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D003574:Cytochrome c Group; D008565:Membrane Proteins; D009284:Naphthols; D045322:Photosynthetic Reaction Center Complex Proteins; D011809:Quinones; D013729:Terpenes; D014812:Vitamin K