Mechanisms involved in the chemoprotective effects of rosemary extract studied in human liver and bronchial cells.
Natural polyphenols found in rosemary have not only potent antioxidant activities but also anticarcinogenic properties. We have studied some of the molecular mechanisms involved in their chemopreventive action using in vitro human liver and bronchial cell models. Rosemary extract, or its active components, carnosol or carnosic acid are potent inhibitors of DNA adduct formation induced by benzo(a)pyrene or aflatoxin B1. At least two mechanisms are involved in the anticarcinogenic action of rosemary extract: (i) inhibition of the metabolic activation of procarcinogens catalysed by the phase I cytochrome P450 enzymes; (ii) induction of the detoxification pathway catalysed by the phase II enzymes such as glutathione S-transferase.
Offord
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EA
Nestlé Research Centre, Lausanne, Switzerland. elizabeth.offord-cavin@chlsnr.nestrd.ch
Macé
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K
Avanti
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O
Pfeifer
A M
AM
D016604:Aflatoxin B1 / Q000494:pharmacology; D015153:Blotting, Western; D001980:Bronchi / Q000187:drug effects / Q000201:enzymology*; D002478:Cells, Cultured; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism*; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D018736:DNA Adducts / Q000096:biosynthesis / Q000187:drug effects; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009153:Mutagens / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D017365:Spices
D018736:DNA Adducts; D009153:Mutagens; D010936:Plant Extracts; D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1; D006899:Mixed Function Oxygenases; C104464:CYP3A protein, human; D019388:Cytochrome P-450 CYP1A2; D051544:Cytochrome P-450 CYP3A; D005982:Glutathione Transferase