title: Airway hyperresponsiveness in a rat model of chronic bronchitis: role of C fibers.
abstract: We evaluated the role of C fibers in the development of airway hyperresponsiveness in a rat model of chronic bronchitis. Neonatal rats were treated with capsaicin (50 mg/kg, subcutaneously), a procedure which results in permanent depletion of tachykinins from the lungs and airways as well as degeneration of C fibers. Control rats were treated with the vehicle used to dissolve capsaicin. Three months later, rats from both groups were exposed either to SO2 gas (250 ppm, 5 h/d, 5 d/wk for 4 wk) or to filtered air for the same period of time. One day after the last exposure, rats were anesthetized and instrumented for the measurement of pulmonary resistance (R(L)), dynamic compliance (Cdyn), and airway responsiveness to inhaled aerosolized methacholine. There was a small (30%) but significant increase in R(L) in neonatal capsaicin- but not vehicle-treated rats exposed to SO2. Chronic exposure to SO2 resulted in increased airway responsiveness in both groups of rats, but the effect was more pronounced in the neonatal capsaicin-treated animals in which the doses of methacholine required to double R(L) or decrease Cdyn by 50% decreased 6.3-fold and 4.6-fold, respectively, compared with only 2.2- and 1.3-fold decreases in vehicle-treated rats. Morphometric analysis of histologic sections of airways demonstrated that the average area of smooth muscle in the airway wall, normalized by the length of basement membrane, was significantly greater in SO2 compared with air-exposed capsaicin-treated rats, but not in vehicle-treated control rats (p < 0.012). The maximal tension generated by tracheal rings in response to cholinergic agonists was also significantly increased by SO2 exposure in neonatal capsaicin-treated, but not vehicle-treated rats (p < 0.002). These results support the hypothesis that rather than contributing to the pathophysiologic manifestations of bronchitis, C fibers limit the development of airway obstruction and airway hyperresponsiveness during induction of chronic bronchitis by SO2 exposure. The enhanced contractile responses of airways from the SO2-exposed neonatal capsaicin-treated rats may result from increased airway smooth muscle mass and contribute to the increased airway responsiveness observed in these animals.
authors.affiliation: Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
authors.forename: N C
authors.initials: NC
authors.lastname: Long
authors.forename: J G
authors.initials: JG
authors.lastname: Martin
authors.forename: R
authors.initials: R
authors.lastname: Pantano
authors.forename: S A
authors.initials: SA
authors.lastname: Shore
mesh_terms: D000818:Animals; D001980:Bronchi / Q000187:drug effects / Q000294:innervation* / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000503:physiopathology*; D001985:Bronchial Provocation Tests; D001991:Bronchitis / Q000139:chemically induced / Q000503:physiopathology*; D016085:Bronchoconstrictor Agents; D002211:Capsaicin / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D016210:Methacholine Chloride; D009412:Nerve Fibers / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015656:Respiratory Mechanics / Q000187:drug effects / Q000502:physiology; D013458:Sulfur Dioxide / Q000633:toxicity; D014132:Trachea / Q000187:drug effects / Q000503:physiopathology
chemical_list: D016085:Bronchoconstrictor Agents; D013458:Sulfur Dioxide; D016210:Methacholine Chloride; D002211:Capsaicin