Expression of the microfilarial sheath protein 2 (shp2) of the filarial parasites Litomosoides sigmodontis and Brugia malayi.
The microfilarial sheaths of the filarial parasites Brugia malayi, Brugia pahangi, and Litomosoides sigmodontis consist of several parasite proteins, probably ranging between 7 and 10. The gene encoding sheath protein 2 (shp2), which is the object of this study, is transcribed in embryos and in the uterine epithelium; at least in B. malayi, it is translated in both tissues. Apparently, shp2 is synthesized as a monomer, exported by the respective cells, and integrated into the microfilarial sheath. In the sheath, it exists as a highly polymerized molecule cross-linked by cysteine formation and other covalent bonds, presumably epsilon-(gamma-glutamyl)-lysine links.
Conraths
F J
FJ
Institut für Parasitologie, Justus-Liebig-Universitat Giessen, Germany. franz.conraths@wus.bfav.de
Hirzmann
J
J
Hobom
G
G
Zahner
H
H
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D017178:Brugia malayi / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005370:Filarioidea / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D015801:Helminth Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D017403:In Situ Hybridization; D008297:Male; D008842:Microfilariae / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013045:Species Specificity; D014158:Transcription, Genetic
D000947:Antigens, Helminth; D015801:Helminth Proteins; D007106:Immune Sera; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C095048:microfilarial sheath protein, Helminth