Pneumostatic effect of gelatin-resorcinol formaldehyde-glutaraldehyde glue on thermal injury of the lung: an experimental study on rats.
OBJECTIVE
Recently, the use of gelatin-resorcinol formaldehyde-glutaraldehyde (GRFG) glue has been reported in vascular surgery, especially in surgery for acute aortic dissection. However, reports concerning its use in lung surgery are quite rare. Although the strong adhesion and tensile strength of GRFG glue to fresh incisional wounds of the lung has been demonstrated experimentally, the effectiveness of this adhesive on thermal injury with severe tissue degeneration has not yet been reported.


METHODS
We experimentally evaluated the ability of GRFG glue to seal air leaks through severely degenerated tissues after thermal injury on rat lung, and compared its performance with two reference adhesives: fibrin glue and EDH-adhesive.


RESULTS
The GRFG glue provided complete pneumostasis immediately after the sealing in the presence of positive pressure ventilation, unlike the other two reference adhesives. The fate and biocompatibility of the three glues were examined histologically at 1 h and 3, 8, and 20 days after treatment. The GRFG glue tightly adhered to the degenerated tissue surface and was gradually fragmented and absorbed. The healing process was favorable, indicating good biocompatibility. Local tissue irritability was negligible.


CONCLUSIONS
Even in the presence of tissue degeneration and positive pressure ventilation, the GRFG glue has proved efficacious as a surgical adhesive in lung surgery because of its ability to bind tissue rapidly and tightly.
Kodama
K
K
Department of Thoracic Surgery, Center for Adult Diseases, Osaka, Japan.
Doi
O
O
Higashiyama
M
M
Yokouchi
H
H
D000818:Animals; D002059:Burns, Inhalation / Q000473:pathology / Q000601:surgery*; D003485:Cyanates / Q000494:pharmacology; D003487:Cyanoacrylates / Q000494:pharmacology; D004338:Drug Combinations; D005557:Formaldehyde / Q000494:pharmacology*; D005780:Gelatin / Q000494:pharmacology*; D005976:Glutaral / Q000494:pharmacology*; D008168:Lung / Q000473:pathology / Q000601:surgery; D055370:Lung Injury; D008422:Materials Testing; D008478:Mediastinal Emphysema / Q000473:pathology / Q000601:surgery*; D011650:Pulmonary Alveoli / Q000293:injuries / Q000473:pathology / Q000601:surgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D012118:Resorcinols / Q000494:pharmacology*; D014014:Tissue Adhesives / Q000494:pharmacology*
D003485:Cyanates; D003487:Cyanoacrylates; D004338:Drug Combinations; D012118:Resorcinols; D014014:Tissue Adhesives; C083703:gelatin-resorcin-formaldehyde-glutaraldehyde adhesive; D005557:Formaldehyde; C010867:EDH adhesive; D005780:Gelatin; D005976:Glutaral