Preparation of surface-modified albumin nanospheres.
Surface-modified human serum albumin (HSA) nanospheres with a size of around 100 nm in diameter were prepared from poly(amidoamine)-poly(ethylene glycol) copolymer grafted human serum albumin (HSA-PAA-PEG) and poly(thioetheramido acid)-poly(ethylene glycol) copolymer grafted human serum albumin (HSA-PTAAC-PEG). The nanospheres were produced using a pH-coacervation method and cross-linked with glutaraldehyde. The cross-linking efficiency was affected by the type of albumin conjugate used. The zeta potential of the surface-modified nanospheres was significantly lower than that of unmodified particles. The existence of a hydrated steric barrier surrounding the nanospheres was confirmed by electrolyte- and pH-induced flocculation tests. The surface-modified nanospheres showed a reduced plasma protein adsorption on the particle surface compared with unmodified particles.
Lin
W
W
Department of Pharmaceutical Sciences, University of Nottingham, UK.
Garnett
M C
MC
Davies
M C
MC
Bignotti
F
F
Ferruti
P
P
Davis
S S
SS
Illum
L
L
D000327:Adsorption; D001798:Blood Proteins; D004337:Drug Carriers; D004573:Electrolytes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D008863:Microspheres; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D012709:Serum Albumin / Q000138:chemical synthesis / Q000737:chemistry*; D000075462:Serum Albumin, Human; D013499:Surface Properties
D001798:Blood Proteins; D004337:Drug Carriers; D004573:Electrolytes; D012709:Serum Albumin; C106417:human serum albumin-poly(amidoamine)-poly(ethylene glycol) copolymer; C106418:human serum albumin-poly(thioetheramido acid)-poly(ethylene glycol) copolymer; D011092:Polyethylene Glycols; D000075462:Serum Albumin, Human