Anchoring of bovine chromosomes 4, 6, 7, 10, and 14 linkage group telomeric ends via FISH analysis of lambda clones.
We report the placement of 34 new microsatellite (ms) markers, isolated from a lambda phage genomic clone library, on the bovine genetic map by linkage to published markers. Five of these markers lie at or near the ends of linkage groups and are used to establish chromosomal coverage and orientation. Fluorescence in situ hybridization (FISH) analysis demonstrates that the linkage groups on the U.S. Meat Animal Research Center (MARC) map extend to the telomeric region of Chromosomes (Chrs) 7 and 10. Linkage groups on Chrs 4, 6, and 14 appear to be less inclusive.
Smith
T P
TP
U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, P.O. Box 166, Clay Center, Nebraska 68933-0166, USA.
Lopez-Corrales
N
N
Grosz
M D
MD
Beattie
C W
CW
Kappes
S M
SM
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D008040:Genetic Linkage; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats
D017931:DNA Primers