Assay for the determination of urinary 8-hydroxy-2'-deoxyguanosine by high-performance liquid chromatography with electrochemical detection.
A high-performance liquid chromatographic procedure with electrochemical detection is described for the determination of urinary 8-hydroxy-2'-deoxyguanosine, a major oxidative DNA lesion induced by radical forming agents. A two-step solid-phase extraction procedure was followed for extracting 8-hydroxy-2'-deoxyguanosine from human urine and the analysis was performed on a RP-18 analytical column under isocratic conditions. The limit of detection of 8-hydroxy-2'-deoxyguanosine in urine was found to be 0.9 nM. The non-invasive assay provides an indirect measurement of oxidative DNA damage.
Germadnik
D
D
Department of Occupational Medicine, University of Vienna/Vienna General Hospital, Austria.
Pilger
A
A
Rüdiger
H W
HW
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine