Identification of a Trypanosoma cruzi antigenic epitope implicated in the infectivity of fibroblast LLC-MK2 cells.
In the present paper we describe the identification of an antigenic epitope that appears to be associated with the surface membrane of the Trypanosoma cruzi parasite, probably implicated in infectivity. Anti-TcMe antibodies inhibited the infectivity of fibroblast LLC-MK2 cells by 34% relative to a preimmune serum. The epitope was specifically recognized by 55% of the sera from 80 chagasic patients. The anti-TcMe antibody immunoprecipitated proteins of about 60 and 40 kDa from epimastigote and cell-culture trypomastigote forms, respectively. It is likely that the 60- and 40-kDa proteins are processed from higher-molecular-weight precursors, since the antibody immunoprecipitated protein fractions in the range of 115-150 kDa from in vitro translation products of poly A+ RNA.
Martín
F
F
Departamento de Biología Molecular, Instituto de Parasitologia y Biomedicina, López Neyra, CSIC, Granada, Spain.
Puertas
C
C
Thomas
M C
MC
Marañón
C
C
Patarroyo
M E
ME
Martín
J
J
Alonso
C
C
López
M C
MC
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D002460:Cell Line; D000939:Epitopes / Q000276:immunology*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D011817:Rabbits; D014349:Trypanosoma cruzi / Q000276:immunology*
D000953:Antigens, Protozoan; D000939:Epitopes