title: Use of enzyme-linked-immunosorbent assay for detection of IgG and IgM antibodies to Fusobacterium necrophorum in cattle.
abstract: An enzyme-linked-immunosorbent assay (ELISA) with HCl heat-extracted antigen of Fusobacterium necrophorum was conducted to detect specific immunoglobulins G and M in infected cattle. The ELISA revealed an increase (> 0.40) in specific IgG in most of the animals with hepatic abscesses but not that in specific IgM. All the lesions were positive for F. necrophorum. These findings indicated that the ELISA for immunoglobulin G detection may prove to be a useful tool for predictive serodiagnosis of F. necrophorum infection in cattle.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kanoe
authors.forename: T
authors.initials: T
authors.lastname: Hirabayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: Y
authors.initials: Y
authors.lastname: Uraoka
authors.forename: S
authors.initials: S
authors.lastname: Taguchi
authors.forename: S
authors.initials: S
authors.lastname: Motoyoshi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005674:Fusobacterium Infections / Q000175:diagnosis; D005675:Fusobacterium necrophorum / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M