title: Partial identification of spirochaetes from two dairy cows with digital dermatitis by polymerase chain reaction analysis of the 16S ribosomal RNA gene.
abstract: Specimens taken postmortem from typical lesions of digital dermatitis in two dairy cows were tested by the polymerase chain reaction (PCR) for the presence of a spirochaetal 16S rRNA gene. Seven different assays detected the gene in the samples from both cows. Two of the PCR products were sequenced and a comparison of the nucleotide sequences revealed that the spirochaete belonged to the genus Treponema and was closely related to Treponema denticola. A PCR specific for the detection of the digital dermatitis-associated treponeme was developed.
authors.affiliation: Department of Bacteriology, Central Veterinary Laboratory, Addlestone, Surrey.
authors.forename: S G
authors.initials: SG
authors.lastname: Rijpkema
authors.forename: G P
authors.initials: GP
authors.lastname: David
authors.forename: S L
authors.initials: SL
authors.lastname: Hughes
authors.forename: M J
authors.initials: MJ
authors.lastname: Woodward
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003872:Dermatitis / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014208:Treponema / Q000145:classification / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S