title: Characterization of the genes encoding a receptor-like histidine kinase and a cognate response regulator from a biphenyl/polychlorobiphenyl-degrading bacterium, Rhodococcus sp. strain M5.
abstract: We report the cloning, sequence, and expression of the bpdS and bpdT genes from Rhodococcus sp. strain M5, which are believed to encode the first two-component signal transduction system in the genus Rhodococcus, which potentially regulates biphenyl/polychlorobiphenyl metabolism in M5. BpdT has a typical responses regulator sequence (209 amino acids; 23 kDa), whereas BpdS, the predicted histidine kinase component, is an unusually large transmembrane protein (1,576 amino acids; 170 kDa) that contains ATP-binding and leucine-rich repeat motifs and some conserved residues of protein kinases. Expression of bpdST, like that of the bpdC1C2BADE degradative operon, is inducible by biphenyl.
authors.affiliation: Biotechnology Research Institute, National Research Council Canada, Montréal, Québec.
authors.forename: D
authors.initials: D
authors.lastname: Labbé
authors.forename: J
authors.initials: J
authors.lastname: Garnon
authors.forename: P C
authors.initials: PC
authors.lastname: Lau
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001713:Biphenyl Compounds / Q000378:metabolism*; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D005809:Genes, Regulator / Q000235:genetics; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011078:Polychlorinated Biphenyls / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics*; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D012240:Rhodococcus / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D008565:Membrane Proteins; D011078:Polychlorinated Biphenyls; D011494:Protein Kinases; D012329:RNA, Bacterial; D012333:RNA, Messenger; C105454:BpdT protein, Rhodococcus sp. M5; C010574:biphenyl; C105453:BpdS protein, Rhodococcus sp. M5