Primary structure and expression of matrilin-2, the closest relative of cartilage matrix protein within the von Willebrand factor type A-like module superfamily.
A mouse cDNA encoding a novel member of the von Willebrand factor type A-like module superfamily was cloned. The protein precursor of 956 amino acids consists of a putative signal peptide, two von Willebrand factor type A-like domains connected by 10 epidermal growth factor-like modules, a potential oligomerization domain, and a unique segment, and it contains potential N-glycosylation sites. A sequence similarity search indicated the closest relation to the trimeric cartilage matrix protein (CMP). Since they constitute a novel protein family, we introduce the term matrilin-2 for the new protein, reserving matrilin-1 as an alternative name for CMP. A 3. 9-kilobase matrilin-2 mRNA was detected in a variety of mouse organs, including calvaria, uterus, heart, and brain, as well as fibroblast and osteoblast cell lines. Expressed human and rat cDNA sequence tags indicate a high degree of interspecies conservation. A group of 120-150-kDa bands was, after reduction, recognized specifically with an antiserum against the matrilin-2-glutathione S-transferase fusion protein in media of the matrilin-2-expressing cell lines. Assuming glycosylation, this agrees well with the predicted minimum Mr of the mature protein (104,300). Immunolocalization of matrilin-2 in developing skeletal elements showed reactivity in the perichondrium and the osteoblast layer of trabecular bone. CMP binds both collagen fibrils and aggrecan, and because of the similar structure and complementary expression pattern, matrilin-2 is likely to perform similar functions in the extracellular matrix assembly of other tissues.
Deák
F
F
Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, P. O. Box 521, Szeged H-6701, Hungary.
Piecha
D
D
Bachrati
C
C
Paulsson
M
M
Kiss
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D064236:Cartilage Oligomeric Matrix Protein; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000235:genetics; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D064235:Matrilin Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011498:Protein Precursors / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D016415:Sequence Alignment; D014018:Tissue Distribution; D014841:von Willebrand Factor / Q000737:chemistry*
D064236:Cartilage Oligomeric Matrix Protein; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C577539:MATN1 protein, human; C577541:MATN2 protein, human; C577540:Matn1 protein, mouse; C577542:Matn2 protein, mouse; D064235:Matrilin Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; C577553:TSP5 protein, human; D014841:von Willebrand Factor