Is HSP70 involved in nitric oxide-induced protection of the heart?
It is known that HSP70 plays an important role in the antiischaemic effect of adaptation to stress. The aim of our study was to verify the hypothesis that nitric oxide (NO) may contribute to the activation of HSP70 synthesis and to enhance thereby the resistance of organism to the ischaemic and reperfusion damages. We observed that heat shock potentiated NO production in the heart. NO formation was completely blocked by the NO synthase inhibitor N omega-nitro-L-arginine (L-NNA). L-NNA also significantly attenuated the heat shock-induced accumulation of HSP70 (by 45% in heart). Both heat shock and NO donor induced time- and concentration-dependent HSP70 synthesis in the culture of human hepatoblastoma cells Hep G2. Prior injection of NO donor (30-100 mg per rat) exerted a dose-dependent protective effect on the isolated heart in ischaemia and reperfusion within 24 hours. We suggest that NO is involved in the activation of HSP70 synthesis which can play an important role in the delayed protective effect of NO donors.
Malyshev IYu
Institute of General Pathology and Pathophysiology, Moscow, Russia.
Malugin
A V
AV
Manukhina
E B
EB
Larionov
N P
NP
Malenyuk
E B
EB
Malysheva
E V
EV
Mikoyan
V D
VD
Vanin
A F
AF
D000818:Animals; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000502:physiology*; D018197:Hepatoblastoma; D006358:Hot Temperature; D006801:Humans; D007501:Iron / Q000494:pharmacology; D008297:Male; D017202:Myocardial Ischemia / Q000201:enzymology*; D009569:Nitric Oxide / Q000096:biosynthesis / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009589:Nitrogen Oxides / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D018840:HSP70 Heat-Shock Proteins; D009589:Nitrogen Oxides; D009569:Nitric Oxide; C073661:dinitrosyl iron complex; D007501:Iron; D019001:Nitric Oxide Synthase