Granulocyte macrophage colony stimulating factor pretreatment induces an increase of rat Kupffer cells with enhanced cytotoxicity in vitro and prevention of tumor outgrowth in vivo.
Male Wag rats were pretreated for 7 days with 1000 U/ml recombinant murine granulocyte macrophage colony stimulating factor (rmGM-CSF). Rat Kupffer cells (KC) were isolated by a enzymatic method. We injected decreasing numbers of CC531 tumor cells in the portal system. Mean KC yield increased from 1.5 +/- 0.2 to 2.2 +/- 0.2 (p < 0.05). Mean percentage of KC-mediated cytotoxicity against CC531 increased from 20.0 +/- 0.5 to 42 +/- 1.0 after rmGM-CSF (p < 0.05). At 1 x 10(5) CC531 tumor cells we demonstrated prevention of formation of small foci of CC52+ tumor cells. We demonstrate increased isolated KC with enhanced cytotoxic capacity after rmGM-CSF. rmGM-CSF induced prevention of minimal residual disease in the rat liver.
Schuurman
B
B
Department of Surgical Oncology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
Koenen
H J
HJ
Beelen
R H
RH
Meyer
S
S
D000818:Animals; D002277:Carcinoma / Q000188:drug therapy; D016923:Cell Death / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology* / Q000627:therapeutic use; D007150:Immunohistochemistry; D007728:Kupffer Cells / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control / Q000556:secondary; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D016179:Granulocyte Colony-Stimulating Factor; C022616:thiazolyl blue; D013936:Thymidine