title: A rapid tissue culture assay for the detection of okadaic acid and related compounds in mussels.
abstract: Okadaic acid, the main toxin of the diarrhoetic shellfish poisoning (DSP) group, shows high cytotoxicity to Buffalo green monkey (BGM) kidney cell cultures after different times according to concentration. A rapid method of detecting the presence of okadaic acid and related compounds in contaminated mussels by means of a cytotoxicity test is presented. The method is based on direct microscopic observation of toxin-induced morphological changes in BGM cell cultures. A high correlation (r = 0.950; P < 0.001) was found between the official mouse bioassay and the cytotoxicity test conducted on naturally contaminated samples of Mytilus galloprovincialis.
authors.affiliation: Reparto Igiene delle Technologie Alimentari Laboratorio Alimenti, Istituto Superiore di Sanità, Roma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Croci
authors.forename: L
authors.initials: L
authors.lastname: Cozzi
authors.forename: A
authors.initials: A
authors.lastname: Stacchini
authors.forename: D
authors.initials: D
authors.lastname: De Medici
authors.forename: L
authors.initials: L
authors.lastname: Toti
mesh_terms: D000818:Animals; D049872:Bivalvia; D002522:Chlorocebus aethiops; D046508:Culture Techniques; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity; D051379:Mice; D019319:Okadaic Acid / Q000032:analysis* / Q000633:toxicity
chemical_list: D008387:Marine Toxins; D019319:Okadaic Acid