title: Extracts from Viscum and Crataegus are cytotoxic against larynx cancer cells.
abstract: The effects of hexanoic extracts of Viscum cruciatum Sieber parasitic on Crataegus monogyna Jacq. (I), Crataegus monogyna Jacq. parasitized with Viscum cruciatum Sieber (II), and Crataegus monogyna Jacq. non-parasitized (III), and of a triterpenes enriched fractions isolated from I, II and III (CFI, CFII, CFIII respectively), on the growth of HEp-2 cells have been evaluated. All the samples demonstrated significant cytotoxic activity against cultured HEp-2 cells, and all of them showed a stronger in vitro activity than 6-mercaptopurine solution used as a positive control. With the hexanoic extracts I, II and III almost similar activity was obtained, but the hexanoic extract I showed comparably better results. Almost complete inhibition was observed with triterpenes-enriched fractions CFI, CFII and CFIII, at the dose 6 micrograms/ml, after 72 h of treatment. The most intense response was obtained with the triterpenes-enriched fraction CFIII (from Crataegus monogyna non-parasitized), where the inhibition was 93%, but the fraction CFI and CFII showed similar inhibition (92% and 83%).
authors.affiliation: Departamento Farmacia y Tecnologia Farmacéutica, Facultad de Farmacia, Sevilla, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Sáenz
authors.forename: M C
authors.initials: MC
authors.lastname: Ahumada
authors.forename: M D
authors.initials: MD
authors.lastname: García
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D007822:Laryngeal Neoplasms; D014784:Mistletoe; D010936:Plant Extracts; D010946:Plants, Medicinal; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts