Stimulation of primed neutrophils by soluble immune complexes.
Soluble IgG-containing immune complexes are unable to initiate oxidant production in unprimed neutrophils. However, priming of the neutrophils with either granulocyte-macrophage colony-stimulating factor (GM-CSF) or cytochalasin B prior to exposure to these complexes results in activation of a rapid and extensive secretion of reactive oxidants. In this study, we have investigated the ability of soluble immune complexes to: (1) induce oxidant generation; (2) bind to the cell surface; and (3) induce Ca2+ transients in neutrophils primed by incubation with GM-CSF or cytochalasin B. Our findings give new insight into the molecular processes involved in the "priming" phenomenon.
Watson
F
F
Department of Biochemistry, University of Liverpool, U.K.
Edwards
S W
SW
D000936:Antigen-Antibody Complex / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D017136:Ion Transport / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects*; D012995:Solubility
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D003571:Cytochalasin B; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D002118:Calcium