Assay of bropirimine in rat plasma by means of robotic solid-phase extraction and high-performance liquid chromatography.
A rapid and simple high-performance liquid chromatography (HPLC) method incorporating automated solid-phase extraction (SPE) is described for the determination of bropirimine, 2-amino-5-bromo-6-phenyl-4(3H)-pyrimidinone, in plasma samples from various species. Using an automated sample processor, plasma samples were loaded onto C18 SPE columns and the drug eluted with ethanol-methylene chloride (10:90, v/v). The extracts were analyzed by reversed-phase HPLC using a C8 column with a mobile phase consisting of acetonitrile-water-trifluoroacetic acid (20:80:0.1, v/v/v). The UV absorbance of the column effluent was monitored at a wavelength of 292 nm. Linear calibration curves were obtained in the range of 0.01 to 22 micrograms/ml. Precision (< or = 4% relative standard deviation) and accuracy (< or = 5% error) were acceptable at the concentrations evaluated. Application of this method to the quantitation of bropirimine in rat plasma for a toxicokinetic/bioavailability study is reported.
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Pharmacia and Upjohn, Kalamazoo, MI 49007, USA.
Walters
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D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D003596:Cytosine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D004355:Drug Stability; D007275:Injections, Intravenous; D007369:Interferon Inducers / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D051381:Rats; D015203:Reproducibility of Results; D012371:Robotics; D012680:Sensitivity and Specificity; D013997:Time Factors
D007369:Interferon Inducers; D003596:Cytosine; C033560:bropirimine