title: Temporal studies on the deposition of complement on human colostrum IgA and serum IgG immobilized on methylated silicon.
abstract: The temporal deposition of selected complement proteins from human serum onto immobilized human colostrum immunoglobulin (Ig)A and human IgG on hydrophobic silicon was studied by ellipsometry-antibody techniques after incubations at 37 degrees C for up to 1 h. In parallel experiments the serum soluble iC3b, C4d, and Bb were detected by enzyme-linked immunosorbent assay techniques. The IgA-coated surfaces showed activation via the alternative pathway, and displayed a lag phase in the deposition of increased amounts of serum proteins, and anti-C3c and antiproperdin. Anti-IgG, -C1q, -C4, -factor H and -factor B were not deposited at any time to IgA surfaces. Upon coating of the surface with IgG, the classical pathway was rapidly activated and bound, then anti-C3c, antiproperdin, and after short serum incubation times, also anti-C1q and anti-IgG. When factor B-depleted or heat-treated sera were used, the observation was that properdin deposited onto IgG-coated surfaces from both. Ellipsometry and antibody techniques offer a convenient and rapid way to indicate the activation of the complement system on solid surfaces and facilitates a time-resolved determination of the activation pathway(s).
authors.affiliation: Laboratory of Applied Physics, Linköping University, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Tengvall
authors.forename: A
authors.initials: A
authors.lastname: Askendal
authors.forename: I
authors.initials: I
authors.lastname: Lundström
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D003126:Colostrum / Q000276:immunology*; D051561:Complement C3 Convertase, Alternative Pathway; D003179:Complement C3b / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism; D015935:Complement C4b; D003170:Complement Pathway, Alternative; D003165:Complement System Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D007074:Immunoglobulin G / Q000097:blood / Q000378:metabolism*; D066298:In Vitro Techniques; D008422:Materials Testing; D008745:Methylation; D010446:Peptide Fragments / Q000378:metabolism; D011247:Pregnancy; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D003181:Complement C4; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D010446:Peptide Fragments; D003179:Complement C3b; D015935:Complement C4b; C032261:complement C4d; D003165:Complement System Proteins; D051561:Complement C3 Convertase, Alternative Pathway; D012825:Silicon