title: Flux Analysis of Glucose Metabolism in Rhizopus oryzae for the Purpose of Increasing Lactate Yields
abstract: A flux analysis of glucose metabolism in the filamentous fungus Rhizopus oryzae was achieved using a specific radioactivity curve-matching program, TFLUX. Glycolytic and tricarboxylic acid cycle intermediates labeled through the addition of extracellular [U-14C]glucose were isolated and purified for specific radioactivity determinations. This information, together with pool sizes and the rates of glucose utilization and end product production, provided input for flux maps of the metabolic network under two different experimental conditions. Based upon the flux analysis of this system, a mutant of R. oryzae with higher lactate and lower ethanol yields than the parent was sought for and found.
authors.affiliation: Division of Biological Sciences, University of Montana, Missoula, Montana, 59812
authors.initials: A
authors.lastname: Longacre
authors.initials: JM
authors.lastname: Reimers
authors.initials: JE
authors.lastname: Gannon
authors.initials: BE
authors.lastname: Wright