title: Hepatobiliary transport of IgA in the golden Syrian hamster (Mesocricetus auratus).
abstract: Do hamsters, like rats, rabbits and mice, possess an hepatocyte 'IgA pump' whereby circulating plasma polymeric IgA (pIgA) is actively transported into bile, against a concentration gradient, via the polymeric Ig receptor or secretory component (SC)? Precipitating antisera, raised against rat Igs and serum proteins, and crossreacting with their hamster homologues, detected hamster SC by immunoelectrophoresis in bile, but not serum. Gel filtration of hamster bile indicated that free SC eluted between IgG and albumin, as for other mammals. Hamster bile IgA was pIgA, and was true secretory IgA (SIgA) by its reaction with anti-SC antiserum and by SDS-PAGE with reduction. Hamster serum IgA comprised both pIgA and IgA monomers. Mean bile-to-serum concentration ratios (B/S) for IgA, IgG, transferrin and albumin, measured by radial immunodiffusion, were 2.65, 0.019, 0.024, and 0.016, respectively, demonstrating strongly selective enrichment of bile in IgA. Human 125I-labelled dimeric IgA was injected into the circulation of five hamsters with cannulated bile ducts; 20% of the [125I]IgA (> 95% precipitable by trichloroacetic acid) was recovered in bile within 5 h, a figure close to that for mice, but smaller than that for rats and rabbits. The data suggest that bile significantly contributes to hamster intestinal SIgA, as shown for rats, rabbits and mice. This could be relevant to studies where hamsters are used as an experimental model for infection by the human intestinal pathogen, Clostridium difficile.
authors.affiliation: Catholic University of Louvain, International Institute of Cellular and Molecular Pathology, Brussels, Belgium. vaerman@mexp.ucl.ac.be
authors.forename: J P
authors.initials: JP
authors.lastname: Vaerman
authors.forename: A
authors.initials: A
authors.lastname: Langendries
mesh_terms: D000818:Animals; D001646:Bile / Q000276:immunology* / Q000378:metabolism; D002850:Chromatography, Gel; D006224:Cricetinae; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D008099:Liver / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008322:Mammals / Q000378:metabolism; D008647:Mesocricetus / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D011817:Rabbits; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism; D013045:Species Specificity; D014168:Transferrin / Q000378:metabolism
chemical_list: D007070:Immunoglobulin A; D012709:Serum Albumin; D014168:Transferrin; C034639:polymeric IgA