[Biological diagnosis of pneumocystis infection. Evaluation and value of a new direct immunofluorescent technique].
Opportunist disease in immunocompromised patients and prematured babies, Pneumocystis carinii pneumonia was rare. It represents today inaugural manifestation of SIDA in 35-40% of cases. The research of the parasite becomes a current analysis for a medical laboratory. Biological diagnosis is based on the show of the parasite in the sampling with classical coloration technics. The limits of these colorations have played a great part in the development of fluorescent monoclonal antibody stains. In this evaluation, we compared, on 100 bronchiolo-alveolar-lavage (BAL), the performances of a new direct fluorescent monoclonal antibody stain recently commercialized (Pneumo Cel IF test B.M.D. Laboratories) with those of two classical colorations: May-Grünwald-Giemsa and Gomori-Grocott. Standard colorations revealed 18 positive cases while direct immunofluorescence technic revealed 20 cases of pneumocystosis. This new test has showed reliability, sensitivity and high specificity due to the utilisation of a monoclonal antibody. The preparation and the reading of the slides are rapid and easy. In spite of a very higher price, direct immunofluorescent should become a choice method for the majority of medical analysis laboratories, by reason of its qualities and the many advantages it offers in comparison with classical colorations.
Rigole
P
P
Laboratoire de Parasitologie-Mycologie (Pr J.P. Dedet), CHU Montpellier, France.
Basset
D
D
Dedet
J P
JP
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis; D011446:Prospective Studies; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013995:Time
Diagnostic biologique de la pneumocystose. Evaluation et intérêt d'une nouvelle technique d'immunofluorescence directe.