Serum-liposome interaction is an oxygen-dependent process.
Measurements of heat dissipation, oxygen concentration and average vesicle size were correlated to study the effect of serum components on different types of liposome. The results indicate that the interaction between serum components and liposomes is exothermic and oxygen dependent, and leads to disruption of vesicles. The dependence of this effect on serum concentration, vesicle surface charge and type of liposome was also evaluated. Serum components did not produce any effect on conventional liposomes in the absence of oxygen. Moreover, in hypoxic conditions the serum-liposome interaction was delayed. Both results suggest that this interaction is an oxygen-dependent event. Finally, we confirmed that sterically stabilised liposomes remain unalterated in the presence of serum.
Foradada
M
M
Unitat de Fisicoquímica, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Manzano
A
A
Roig
T
T
Estelrich
J
J
Bermúdez
J
J
D000818:Animals; D001769:Blood; D002151:Calorimetry; D002478:Cells, Cultured; D008081:Liposomes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D011048:Polarography
D008081:Liposomes; D010100:Oxygen