In vitro culture of Oestrus ovis (Linné 1761) first instar larvae: its application to antiparasitic drug screening.
A culture medium in which 1st instar larvae of Oestrus ovis can survive for up to 2 months has been developed with Dulbecco's Modified Eagle's Medium (DMEM) pH 7.7, penicillin 100 U/ml, streptomycin 100 micrograms ml-1, gentamicin 10 micrograms ml-1 and foetal calf serum (50%) added. Larvae were incubated in flat plastic tissue culture bottles (3 ml of medium) in a 5% CO2 atmosphere at 37 degrees C in darkness. Subsequently an antiparasitic in vitro screening test was developed with moxidectin and closantel. These drugs were not as effective in vitro as in vivo. This might be due to the fact that they cause damage to parasites and host immune responses, then contribute to their death.
Duranton
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Laboratoire associé de physiopathologie respiratoire des ruminants, INRA UO 959, Ecole Nationale Vétérinaire, Toulouse, France.
Dorchies
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P
D000818:Animals; D000900:Anti-Bacterial Agents; D000977:Antiparasitic Agents / Q000633:toxicity*; D004175:Diptera / Q000502:physiology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000502:physiology; D018942:Macrolides / Q000633:toxicity; D012458:Salicylanilides / Q000633:toxicity*
D000900:Anti-Bacterial Agents; D000977:Antiparasitic Agents; D007306:Insecticides; D018942:Macrolides; D012458:Salicylanilides; C023342:closantel; C027837:moxidectin