title: Structural organization and promoter analysis of the bovine cytochrome c oxidase subunit VIIc gene. A functional role for YY1.
abstract: Cytochrome c oxidase (COX) subunit VIIc is one of the nuclear encoded subunits of the 13-subunit holoenzyme that carries out the terminal step in the electron transport chain. We have isolated the gene for this subunit, previously shown to be ubiquitously expressed from a single copy gene in the genome, and show that 167 base pairs of DNA surrounding the transcriptional start site contain the minimal promoter of this gene. This basal promoter contains two YY1 sites and at least one site for NRF-2, which show binding to their cognate factors. Mutation of both YY1 sites eliminates most of the promoter activity. Mutation at the upstream YY1 site significantly reduces the efficiency of transcript initiation at the major start site and thus plays the dominant role in COX7C regulation. COX7C is, thus, the second nuclear gene of COX that is regulated by YY1, suggesting that it is a third common factor, along with NRF-1 and NRF-2, to be associated with COX gene regulation.
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Seelan
authors.forename: L I
authors.initials: LI
authors.lastname: Grossman
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000235:genetics*; D050981:Erythroid-Specific DNA-Binding Factors; D051268:GA-Binding Protein Transcription Factor; D015971:Gene Expression Regulation, Enzymologic; D006367:HeLa Cells; D006801:Humans; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D051265:NF-E2-Related Factor 1; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics*; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D015183:Restriction Mapping; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D050986:YY1 Transcription Factor; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D003576:Electron Transport Complex IV; D050981:Erythroid-Specific DNA-Binding Factors; D051268:GA-Binding Protein Transcription Factor; D051265:NF-E2-Related Factor 1; D009687:Nuclear Proteins; D051266:Nuclear Respiratory Factor 1; D051264:Nuclear Respiratory Factors; D015534:Trans-Activators; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C105520:COX7C protein, human; C495633:NRF1 protein, human; C494698:YY1 protein, human