title: Chemical modification of dehalogenase.
abstract: Dehalogenase is an enzyme which catalyzes alpha-haloacid into alpha-hydroxyl acid. Chemical modification reactions of dehalogenase YL, 109, and H-2 were carried out by various reagents. The experimental results showed that the activity was not significantly altered by seryl, lysyl, and trpyl reagents but decreased by hisyl, arginyl, and carboxyl reagents. A substitute could protect the active center to chemical modification. It was indicated that hisyl, arginyl, and carboxyl (Glu or Asp) residues would be essential for the activity of dehalogenase.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: N
authors.initials: N
authors.lastname: Jiang
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: K
authors.initials: K
authors.lastname: Soda
mesh_terms: D006867:Hydrolases / Q000737:chemistry*; D007202:Indicators and Reagents; D011549:Pseudomonas / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D007202:Indicators and Reagents; D006867:Hydrolases; C034507:2-haloacid dehalogenase; C023098:haloacetate dehalogenase