title: In vivo evaluation of platelet activation by different cellulosic membranes.
abstract: This study was undertaken to evaluate platelet activation in vivo induced by different cellulosic membranes by measuring the expression of P-selectin on the platelet surface during hemodialysis in 9 uremic patients. Hollow fiber dialyzers of similar surface with different cellulosic membranes (Cuprophan, cellulose acetate, cellulose triacetate, and Hemophan) were evaluated and compared to a synthetic membrane (polysulfone). Blood samples were obtained before hemodialysis and from the efferent and afferent limbs 5 min after the beginning of dialysis. P-selectin exposure was evaluated by flow cytometry (FACScan) using a monoclonal antibody (RUU 2.17). The percentage of platelets expressing P-selectin before hemodialysis and the percentage from the arterial line during hemodialysis were similar. All membranes evaluated induced platelet activation (estimated as the increase in percentage of platelets expressing P-selectin in samples obtained from the venous line with respect to the arterial line). Cuprophan induced more platelet activation than any other membrane (p < 0.05). The activation induced by cellulose acetate and cellulose triacetate membranes was also higher than that observed with Hemophan (p < 0.05). Hemophan-induced platelet activation was similar to that of polysulfone. These results indicate that all cellulosic membranes induce platelet activation during hemodialysis although there are quantitative differences among them. While Cuprophan induced the highest degree of platelet activation, Hemophan was the only cellulosic membrane that showed a degree of platelet activation similar to the biocompatible membrane polysulfone.
authors.affiliation: Nephrology Services, Hospital Clinic I Provincial, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Cases
authors.forename: J C
authors.initials: JC
authors.lastname: Reverter
authors.forename: G
authors.initials: G
authors.lastname: Escolar
authors.forename: C
authors.initials: C
authors.lastname: Sanz
authors.forename: J
authors.initials: J
authors.lastname: Sorribes
authors.forename: A
authors.initials: A
authors.lastname: Ordinas
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001672:Biocompatible Materials / Q000009:adverse effects; D001792:Blood Platelets / Q000378:metabolism; D002482:Cellulose / Q000009:adverse effects* / Q000031:analogs & derivatives; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D019007:P-Selectin / Q000096:biosynthesis / Q000097:blood; D015539:Platelet Activation / Q000187:drug effects* / Q000502:physiology; D011108:Polymers / Q000009:adverse effects; D006435:Renal Dialysis; D013450:Sulfones / Q000009:adverse effects; D013927:Thrombosis / Q000139:chemically induced
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D019007:P-Selectin; D011108:Polymers; D013450:Sulfones; C054645:Hemophan; C017662:polysulfone P 1700; C005062:acetylcellulose; D002482:Cellulose; C024671:cellulose triacetate; C009865:cuprammonium cellulose