Chromatographic analysis of the enantiomers of ifosfamide and some of its metabolites in plasma and urine.
The enantiomers of the cytostatic drug ifosfamide and the two metabolites 2- and 3-dechloroethylifosfamide were isolated from plasma and urine by liquid-liquid extraction with ethyl acetate, resolved on a Chirasil-L-val gas chromatographic column and detected by a nitrogen-phosphorus-selective flame ionisation detector. Resolution of the racemic compounds for identification purposes was also accomplished with high-performance liquid chromatography on a chiral column. The validated gas chromatographic method was suitable to determine the total concentrations and the enantiomeric composition of ifosfamide and its dechloroethylated metabolites in plasma and urine samples from treated patients. Some metabolic preferences in the metabolism of ifosfamide were found.
Kaijser
G P
GP
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, Netherlands.
Beijnen
J H
JH
Bult
A
A
Keizer
H J
HJ
Underberg
W J
WJ
D000328:Adult; D018906:Antineoplastic Agents, Alkylating / Q000097:blood* / Q000627:therapeutic use / Q000652:urine*; D002277:Carcinoma / Q000097:blood / Q000188:drug therapy / Q000652:urine; D002849:Chromatography, Gas; D003520:Cyclophosphamide / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D006801:Humans; D007069:Ifosfamide / Q000031:analogs & derivatives / Q000097:blood* / Q000627:therapeutic use / Q000652:urine*; D008297:Male; D012004:Rectal Neoplasms / Q000097:blood / Q000188:drug therapy / Q000652:urine; D013237:Stereoisomerism
D018906:Antineoplastic Agents, Alkylating; C064445:dechloroethylcyclophosphamide; D003520:Cyclophosphamide; C038007:dechloroethylifosfamide; D007069:Ifosfamide