title: The recA gene from Clostridium perfringens is induced by methyl methanesulphonate and contains an upstream Cheo box.
abstract: The recA gene from Clostridium perfringens was cloned using degenerate oligonucleotide primers designed from conserved regions of RecA proteins from other bacteria. The 1089 bp gene encoded a putative RecA protein with 69% amino acid sequence similarity to the RecA protein from Bacillus subtilis. The C. perfringens recA gene was induced by exposure to methyl methanesulphonate and complemented a recA mutant of Escherichia coli. A cheo box was identified in the region upstream of the gene. Since this SOS-like operator site is conserved in many DNA-damage-inducible recA gene regions from Gram-positive bacteria, the results suggest that the regulation of the C. perfringens recA gene also involves the binding of a LexA-like protein to this site.
authors.affiliation: Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Johnston
authors.affiliation: Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
authors.forename: Joan
authors.initials: J
authors.lastname: Sloan
authors.affiliation: Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
authors.forename: Janet A M
authors.initials: JAM
authors.lastname: Fyfe
authors.affiliation: Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
authors.forename: John K
authors.initials: JK
authors.lastname: Davies
authors.affiliation: Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
authors.forename: Julian I
authors.initials: JI
authors.lastname: Rood
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D011938:Rec A Recombinases / Q000235:genetics*; D017421:Sequence Analysis; D013450:Sulfones / Q000494:pharmacology*
chemical_list: D013450:Sulfones; D008741:Methyl Methanesulfonate; D011938:Rec A Recombinases