[Bacteriological detection methods of enterohemorrhagic Escherichia coli].
A rapid identification of enterohemorrhagic Escherichia coli is critical to diagnose and treat its infection due to a possible development of hemorrhagic colitis, hemolytic uremic syndrome and cerebral symptom. For that, several bacteriological detection methods are developed and some of them have been used in clinical microbiology laboratories. In this article, classical bacteriological methods, immunological methods and PCR for identification of enterohemorrhagic E. coli are reviewed and advantages as well as disadvantages of various methods are discussed.
Takeda
Y
Y
Research Institute, International Medical Center of Japan.
D001427:Bacterial Toxins / Q000032:analysis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000145:classification / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D007841:Latex Fixation Tests; D016133:Polymerase Chain Reaction; D012703:Serotyping; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D022622:Shiga Toxin 1