T-lymphocytes from individuals with filarial inflammatory disease have increased transendothelial migration in vitro.
The in vitro transendothelial migration of circulating filarial antigen-specific T-cells was examined in Wuchereria banerofti infection. Circulating T-cells from individuals with filaria-induced lymphatic pathology (LP) had significantly greater migration through unstimulated HUVEC monolayers than did T-cells from asymptomatic infected (MF) individuals (P = 0.04). In contrast to the MF individuals where no effect was seen, transendothelial migration of 48-hr filarial antigen stimulated T-cells from LP individuals was significantly (P = 0.01) greater than migration of 48-hr media-stimulated T-cells. In six of seven patients examined, inhibition of the VLA-4/VCAM-1 pathway resulted in greater than 50% inhibition of transendothelial migration of T-cells.
Plier
D A
DA
Department of Medicine, University of Alabama at Birmingham, USA.
Maia e Silva
M C
MC
Maciel
M A
MA
Freedman
D O
DO
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D001667:Binding, Competitive; D002465:Cell Movement / Q000187:drug effects; D004605:Elephantiasis, Filarial / Q000473:pathology*; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D006801:Humans; D039041:Integrin alpha4beta1; D016023:Integrins / Q000502:physiology; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D016201:Receptors, Lymphocyte Homing / Q000502:physiology; D016029:Receptors, Very Late Antigen / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology*; D014471:Umbilical Veins / Q000166:cytology; D019010:Vascular Cell Adhesion Molecule-1 / Q000494:pharmacology; D014958:Wuchereria bancrofti
D000911:Antibodies, Monoclonal; D039041:Integrin alpha4beta1; D016023:Integrins; D016328:NF-kappa B; D016201:Receptors, Lymphocyte Homing; D016029:Receptors, Very Late Antigen; D019010:Vascular Cell Adhesion Molecule-1