Research on fermentation scale-up based on the OUR obtained from a shake flask.
On the basis of determination of oxygen permeability Pm of gauze layers covered on a specially designed shake flask by the method of sulfite oxidation, the variations of gas-phase and liquid-phase oxygen concentration were measured during the course of fermentation and then the oxygen uptake rate (OUR) and volumetric oxygen transfer coefficient (KLa) could be determined too. Fermentation process and fermentor scale-up were carried out based on the (OUR)max obtained from the shake flask. Scale-up process was studied in different air flow rates and agitation speeds in a 2.5 L fermentor with the analyses by mass spectrometer and manual sampling, it was found that OUR and biomass obtained from the fermentor were quite close to that obtained from the shake flask, but quite different in KLa and CL.
Fan
D
D
Chemical Engineering Department, Northwest University, Xi'an, China.
Shang
L
L
Yu
J
J
D019149:Bioreactors; D005285:Fermentation; D010100:Oxygen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D010100:Oxygen