title: Importance of 2-O-sulfate groups of uronate residues in heparin for activation of FGF-1 and FGF-2.
abstract: Complete drying of heparin with various concentrations of NaOH by lyophilization caused specific 2-O-desulfation to various degrees without detectable depolymerization or other chemical changes. In order to assess the importance of 2-O-sulfate groups in uronate residues to promote FGF-1 and FGF-2 activities, various 2-O-desulfated (2-O-DS-) heparins were quantitatively examined for their effects on FGF-1 and FGF-2-induced proliferation of BALB/c3T3 clone A31 (A31) cells and the chlorate-treated cells. Twenty-seven percent or less loss to the 2-O-sulfate groups had no effect on the ability to activate both FGF-1 and FGF-2, while 44% loss resulted in a significant loss of the ability. Complete loss of the ability was observed in 2-O-DS-heparins with 75% or more 2-O-desulfation. These results suggest that a high content of 2-O-sulfate groups in uronate residues of heparin is required for activation of both FGF-1 and FGF-2.
authors.affiliation: National Defense Medical College, Research Institute, Saitama.
authors.forename: M
authors.initials: M
authors.lastname: Ishihara
authors.forename: Y
authors.initials: Y
authors.lastname: Kariya
authors.forename: H
authors.initials: H
authors.lastname: Kikuchi
authors.forename: T
authors.initials: T
authors.lastname: Minamisawa
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D016475:3T3 Cells; D000818:Animals; D019556:COS Cells; D002455:Cell Division; D002704:Chlorates / Q000494:pharmacology; D002999:Clone Cells / Q000378:metabolism; D004187:Disaccharides / Q000737:chemistry; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005612:Freeze Drying; D006493:Heparin / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000378:metabolism*; D014574:Uronic Acids / Q000378:metabolism*
chemical_list: D002704:Chlorates; D004187:Disaccharides; D013431:Sulfates; D014574:Uronic Acids; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D006493:Heparin