title: Medial elastic structure alterations in atherosclerotic arteries in minipigs: plaque proximity and arterial site specificity.
abstract: Using a model of atherosclerosis in minipigs, we analyzed changes in elastic structure within the medial sections of the abdominal aorta and left interventricular coronary artery both in the vicinity of and distal to atheromatous plaques. Twenty-four animals, divided into three groups, were fed either a control diet or a hypercholesterolemic and hyperhomocysteinic atherogenic diet, alone or in association with an antihypertensor, namely isosorbide dinitrate (Risordan). The atherogenic diet, administered for a period of four months, induced in the minipig advanced noncalcified atherosclerotic lesions that were histologically similar to those found in humans. A morphodensitometric analysis of the medial elastic structures was carried out on images obtained from specifically stained transverse arterial sections examined under a light microscope. The volume density of the elastic structures was diminished in the arterial media of the atherosclerotic animals due to opening and widening of the fenestrae in the elastic laminate and increased communication between the interlamellar spaces. Whereas this elastolytic process was uniform and independent of the proximity of atheromatous plaques in the left interventricular coronary artery, it was intensified in the vicinity of the plaques in the abdominal aorta. Overall elastolytic activity was increased in the walls of atheromatous artery in both arterial sites, and metalloproteinases were implied in this increase of activity. We previously reported that treatment with isosorbide dinitrate significantly reduced the moderate systolic hypertension and the increase in transparietal stress observed in the abdominal aorta of atheromatous animals. We report here that isosorbide dinitrate prevented the atherogenic-diet-induced deterioration of the elastic structure in these arteries; complete inhibition of changes to the elastic laminae was evident in areas remote from plaque formation, but only partial inhibition in the vicinity of such plaques. It did not, however, prevent structural damage in the left interventricular coronary artery or modify the increase in parietal elastolytic activity in either of the two arteries. This suggests that damage to the elastic structure in atheromatous arteries is dependent not only on overall elastolytic activity but also on localized factors, possibly related to parietal stresses, affected by the presence of atheromatous plaques.
authors.affiliation: C.J.F. INSERM 94-01 Biochemistry Lab, School of Pharmacy, Marseille, France.
authors.forename: T
authors.initials: T
authors.lastname: Augier
authors.forename: P
authors.initials: P
authors.lastname: Charpiot
authors.forename: C
authors.initials: C
authors.lastname: Chareyre
authors.forename: M
authors.initials: M
authors.lastname: Remusat
authors.forename: P H
authors.initials: PH
authors.lastname: Rolland
authors.forename: D
authors.initials: D
authors.lastname: Garçon
mesh_terms: D000596:Amino Acids / Q000097:blood / Q000378:metabolism; D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology; D001158:Arteries / Q000201:enzymology; D001161:Arteriosclerosis / Q000150:complications*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D003331:Coronary Vessels / Q000473:pathology; D003720:Densitometry; D004036:Diet, Atherogenic; D004547:Elastic Tissue / Q000473:pathology*; D004549:Elastin / Q000032:analysis; D008297:Male; D010196:Pancreatic Elastase / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature
chemical_list: D000596:Amino Acids; D004549:Elastin; D002784:Cholesterol; D010196:Pancreatic Elastase