title: Decreased endotoxin-binding capacity of whole blood in patients with alcoholic liver disease.
abstract: OBJECTIVE
  The proinflammatory effects of endotoxemia, which is often observed in alcohol-abusing patients with various degrees of liver disease, may be modulated by changes in the concentration of endotoxin binding factors. Therefore, the plasma endotoxin concentration and the overall endotoxin binding capacity of whole blood were measured in these patients.
  
  
  METHODS
  Patients with minor (A1; n=27), more pronounced (A2; n=13), cirrhotic alcoholic liver disease (A3; n=18), and non-alcoholic cirrhosis (NC; n=6), and 15 healthy control persons (HC) were included in the study. Endotoxin plasma levels were determined using a standardized limulus assay. A modified assay was applied to additionally detect tightly bound endotoxin. To measure the endotoxin-binding capacity, aliquots of whole blood were incubated with serial dilutions of endotoxin, supernatants were obtained, and endotoxin retrieval was estimated by addition of limulus lysate, followed by photometric measurement of the maximal reaction velocity (dODmax). Endotoxin binding capacity equals the endotoxin concentration at which dODmax reaches a predefined threshold.
  
  
  RESULTS
  All groups of alcohol abusers had significantly elevated endotoxin plasma levels with a considerable portion of 'bound' endotoxin. Conversely, the endotoxin binding capacity was markedly diminished, mainly in patients with more advanced liver disease (A1: 85.8% of the control value [non-significant vs. controls]; A2: 25.4% [p<0.05]; A3: 43.6% [p<0.02], NC: 43.2%).
  
  
  CONCLUSIONS
  The endotoxin-binding capacity is diminished in patients with alcoholic and non-alcoholic cirrhosis, as well as in less advanced alcoholic liver disease. Reduced endotoxin binding may contribute to the adverse effects of endotoxemia.
authors.affiliation: Department of Internal Medicine, Hohenheim University, Stuttgart, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Schäfer
authors.forename: B
authors.initials: B
authors.lastname: Greiner
authors.forename: J
authors.initials: J
authors.lastname: Landig
authors.forename: E
authors.initials: E
authors.lastname: Feil
authors.forename: E T
authors.initials: ET
authors.lastname: Schütz
authors.forename: J C
authors.initials: JC
authors.lastname: Bode
authors.forename: C
authors.initials: C
authors.lastname: Bode
mesh_terms: D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000009:adverse effects; D001665:Binding Sites; D002908:Chronic Disease; D019446:Endotoxemia / Q000097:blood / Q000175:diagnosis / Q000209:etiology; D004731:Endotoxins / Q000097:blood*; D004926:Escherichia coli; D005260:Female; D006801:Humans; D008033:Limulus Test; D008104:Liver Cirrhosis, Alcoholic / Q000097:blood / Q000150:complications / Q000175:diagnosis; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000150:complications / Q000175:diagnosis; D008108:Liver Diseases, Alcoholic / Q000097:blood* / Q000150:complications / Q000175:diagnosis; D008297:Male; D008875:Middle Aged
chemical_list: D004731:Endotoxins