In vitro influence of ascorbate on lipid peroxidation in rat testis and heart microsomes.
Lipid peroxidation (LPO) in rat testis and heart microsomes was compared using the ADP/Fe2+ as initiator with and without ascorbate at different concentrations. The extent of LPO was estimated by the levels of TBARS and PUFA. Without ascorbate, LPO was higher in heart than in testis despite elevated levels of catalase in heart. With increased ascorbate concentrations, a biphasic effect of LPO was observed. For a concentration < or = 0.2 mM, ascorbate acted as pro-oxidant and increased TBARS correlated with decreased PUFA were observed both in testis and heart. Above 0.2 mM, ascorbate acts as antioxidant but differences in the rate of LPO were observed. In heart decreased TBARS correlated with increased PUFA whereas in testis TBARS only decreased, PUFA were not significantly modified. These results suggest different mechanisms in LPO initiation in the two organs. Increasing concentrations of H2O2 produced directly elevated TBARS levels in testis while a lag phase was observed in heart before the increase, suggesting that H2O2 was the essential ROS produced by ascorbate-ADP/Fe2+. The effects of scavengers such as catalase and ethanol showed an inhibitory effect on TBARS production only in testis, suggesting the role of H2O2/OH. as an initiator of LPO. In heart, catalase produced a slight increase in TBARS levels whereas no modification was observed with ethanol, suggesting a possible direct activation by ADP/Fe2+ through a metal-oxo intermediate.
Melin
A M
AM
Laboratoire de Biochimie Médicale A, Université de Bordeaux II, France.
Peuchant
E
E
Perromat
A
A
Clerc
M
M
D000244:Adenosine Diphosphate; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D016166:Free Radical Scavengers; D006321:Heart / Q000187:drug effects*; D006861:Hydrogen Peroxide; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D005231:Fatty Acids, Unsaturated; D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D000244:Adenosine Diphosphate; D006861:Hydrogen Peroxide; D007501:Iron; D001205:Ascorbic Acid