Immunoassay reagents for thyroid testing. 2. Binding properties and energetic parameters of a T4 monoclonal antibody and its Fab fragment with a library of thyroxine analog biosensors using surface plasmon resonance.
A library of 16 thyroxine analog biosensors were prepared for use on the BIAcore surface plasmon resonance instrument. An anti-thyroxine monoclonal antibody and its Fab fragment were tested with each biosensor to generate the association and dissociation rate constants, equilibrium association constants, and Gibbs free energy values for characterizing the binding event.
Adamczyk
M
M
Division Organic Chemistry (9-NM), Abbott Laboratories, Abbott Park, Illinois 60064, USA. adamczykm@apmac.abbott.com
Gebler
J C
JC
Gunasekera
A H
AH
Mattingly
P G
PG
Pan
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D015374:Biosensing Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007140:Immunoglobulin Fab Fragments; D007202:Indicators and Reagents; D007700:Kinetics; D013058:Mass Spectrometry; D015394:Molecular Structure; D013816:Thermodynamics; D013960:Thyroid Function Tests / Q000379:methods*; D013974:Thyroxine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007202:Indicators and Reagents; D013974:Thyroxine