The usefulness of anti-fucosylated antigen antibody YB-2 for diagnosis of hepatocellular carcinoma.
Levels of fucosylated antigens in sera from patients with liver diseases were examined by a newly developed sandwich-type enzyme immuno assay with the aid of anti-fucosylated antigen antibody, YB-2 which reacts simultaneously with Y, Leb and H type 2 antigens. When the cut-off value was set arbitrarily at mean +3 SD values of normal, 30 (69.8%) of the 43 patients with HCC, 14 (53.8%) of the 26 patients with liver cirrhosis (LC) and 24 (45.3%) of the 53 patients with chronic hepatitis (CH) were found to be positive, whereas all of the 30 samples from healthy controls were negative. The levels of alpha-fetoprotein (AFP) and protein induced by vitamin K absence or antagonist-II (PIVKA-II) in HCC were not correlated with those of YB-2 antigens. The positive rates of the combination YB-2 and AFP assay and YB-2 and PIVKA-II assay in HCC were significantly higher (83.7 and 86.0%, respectively) than that of the AFP and PIVKA-II combination (65.1%) which had been reported to be the best combination up to this time.
Nakamura
J
J
First Department of Surgery, Gunma University School of Medicine, Maebashi, Japan.
Yazawa
S
S
Hada
T
T
Asao
T
T
Naitoh
H
H
Takenoshita
S
S
Kosaka
M
M
Akamatsu
S
S
Tachikawa
T
T
Nagamachi
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology*; D000941:Antigens / Q000097:blood* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000097:blood / Q000276:immunology; D015415:Biomarkers; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006505:Hepatitis / Q000097:blood / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008103:Liver Cirrhosis / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011498:Protein Precursors / Q000032:analysis; D011516:Prothrombin / Q000032:analysis; D012016:Reference Values; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D000941:Antigens; D000951:Antigens, Neoplasm; D015415:Biomarkers; D011498:Protein Precursors; D000509:alpha-Fetoproteins; C008208:acarboxyprothrombin; D011516:Prothrombin