title: Using computer simulated results of a bulk drug substance assay to determine acceptance criteria for method validation.
abstract: OBJECTIVE
  To determine the statistical variability expected for a well designed HPLC assay of a bulk drug substance (BDS). The results are used to develop appropriate acceptance criteria for a method validation protocol as well as to evaluate the level of uncertainty expected for assay results using a variety of sampling/injection schemes.
  
  
  METHODS
  Computer simulation was used to generate a large quantity of data and the variability of the mock results was evaluated. Error propagation was also calculated, whenever possible, to confirm results obtained from the simulations.
  
  
  RESULTS
  Protocol acceptance criteria were developed that were consistent with the expected variability for data resulting from the execution of the validation protocol. In certain cases simulations provided the only avenue of obtaining results that could not otherwise be readily determined.
  
  
  CONCLUSIONS
  Computer modeling can be used to obtain suitable acceptance criteria for validation results which are consistent with method variability. This is particularly significant in the case of linearity where it has been difficult to develop acceptance criteria based on anything other than analyst intuition and experience. Assay simulations clearly demonstrated that the variability expected for a typical BDS assay is large relative to the average specification range and therefore little insight about relative purity can be gained comparing individual passing assay results.
authors.affiliation: Preformulation Department, Allergan, Irvine, California 92713, USA.
authors.forename: R D
authors.initials: RD
authors.lastname: Bunnell
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D003198:Computer Simulation; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D004364:Pharmaceutical Preparations