Refolding of soluble leukemia inhibitory factor receptor fusion protein (gp 190 sol DAF) from urea.
The insoluble inclusion bodies of soluble leukemia inhibitory factor receptor fusion protein (gp 190 sol DAF) was solubilized in 8 M urea on the unfolding transitions, and several factors on the aggregate formation were indirectly analyzed for the refolding of gp 190 sol DAF. Results indicate that the refolding yield can be considerably increased at lowering concentration of the unfolding protein, a little soluble protein with the slow refolding appears in the process of the aggregate formation and the concentration of the denaturant must be down to a minimum level for its refolding.
Liu
H
H
Medical Molecular Biology Laboratory, Second Military Medical University, Shanghai, Peoples Republic of China.
Moreau
J F
JF
Gualde
N
N
Fu
J
J
D005990:Glycerol / Q000494:pharmacology; D006131:Growth Inhibitors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines; D008970:Molecular Weight; D017510:Protein Folding; D018121:Receptors, Cytokine / Q000737:chemistry* / Q000187:drug effects; D053606:Receptors, OSM-LIF; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000187:drug effects; D014508:Urea / Q000737:chemistry
D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; C507840:LIFR protein, human; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF; D011993:Recombinant Fusion Proteins; D014508:Urea; D005990:Glycerol