A genetic sequence change in the 3'-noncoding region of the androgen receptor gene in prostate carcinoma.
OBJECTIVE
We intended to identify structural changes in the hormone-binding domain and C-terminal region of the androgen receptor (AR) gene in carcinoma of prostate specimens.


METHODS
Twelve prostate cancer specimens from prostate resections were analyzed for the presence of mutations in the E-H exons and part of the 3'-untranslated region of the AR cDNA, using RT-PCR and direct sequencing.


RESULTS
A polymorphic sequence spanning 17 nucleotides was identified in the 3'-untranslated region of the AR cDNA from one prostate carcinoma tissue, suggesting the presence of a somatic mutation. No mutations were identified in the coding sequence of the hormone-binding domain of the AR cDNA.


CONCLUSIONS
These results suggest that AR mutations are probably uncommon molecular events in the early stages of prostate cancer.
Paz
A
A
Urology Department, Assaf Harofeh Medical Center, Zerifin, Israel.
Lindner
A
A
Zisman
A
A
Siegel
Y
Y
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000368:Aged; D000483:Alleles; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005091:Exons; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D012333:RNA, Messenger / Q000032:analysis; D011944:Receptors, Androgen / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D011944:Receptors, Androgen