Fatty acid accumulation in the yeast Sporidiobolus salmonicolor during batch production of gamma-decalactone.
This paper provides new information about the metabolism of various fatty acids and gamma-decalactone production by yeast. An analysis of the fatty acid composition of the yeast Sporidiobolus salmonicolor during batch production of lactone with ricinoleic acid methyl ester as a precursor showed an accumulation of the gamma-decalactone precursor inside the cells. Electron microscopy of the yeasts showed the presence of large internal inclusions leading to membrane and organelle lysis and, consequently, death of the yeast. S. salmonicolor cultivated with methyl oleate did not produce gamma-decalactone and is viable during the whole culture. Analysis of the long chain fatty acid fraction showed incorporation of methyl oleate.
Feron
G
G
Laboratoire de Recherches sur les Arômes, Institut National de la Recherche Agronomique, Dijon, France. feron@arom0.dijon.inra.fr
Dufossé
L
L
Mauvais
G
G
Bonnarme
P
P
Spinnler
H E
HE
D005227:Fatty Acids / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D007783:Lactones / Q000737:chemistry / Q000378:metabolism*; D008828:Microbiological Techniques; D008854:Microscopy, Electron; D019301:Oleic Acid / Q000378:metabolism / Q000494:pharmacology; D012277:Ricinoleic Acids / Q000378:metabolism / Q000494:pharmacology; D015003:Yeasts / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D005227:Fatty Acids; D005656:Fungal Proteins; D007783:Lactones; D012277:Ricinoleic Acids; D019301:Oleic Acid; C005235:methyl ricinoleate