ABO genotyping by PCR-direct sequencing.
The PCR-direct sequence method was applied to ABO genotyping. At the 261st nucleotide of the genes of A and B glycosyltrasferase, it was easily detected that the nucleotide was guanine in AA, AB and BB genotypes and that the nucleotide was ademine in only OO. In AO and BO, substitution of A to G was confirmed by the dye primer method, but it was difficult to detect correctly by the dye terminator method. At the 297th, nucleotide substitution between A and B alleles was confirmed by the both methods. As this position, O allele was subdivided into three types, OAOA, OGOG and OAOG. At the 703rd, nucleotide substitution between A and B alleles was easily detected by the both methods. The PCR-direct sequence method was suitable to confirm the nucleotide substitution or deletion directly and to prevent the mistyping by other methods.
Nata
M
M
Department of Forensic Medicine, Tohoku University, School of Medicine, Sendai.
Kanetake
J
J
Adachi
N
N
Hashiyada
M
M
Aoki
Y
Y
Sagisaka
K
K
D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D005838:Genotype; D016695:Glycosyltransferases / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D000017:ABO Blood-Group System; D016695:Glycosyltransferases