[Intravital and electron microscopic analysis of the arterioles by immersion and perfusion technics of fixation of the rat mesentery].
The technique of the intravital microscopic control, videotaping, cinemoregistration and per single-frame analysis made it possible to demonstrate for the first time that a double fixation in glutaraldehyde and osmium has no effect on the arteriolar lumen. Comparing the data on the intravital and a detailed electron microscopic study, it was possible to reveal certain peculiarities and advantages of the fixative perfusion and immersion techniques applied. The investigation establishes a basis for extended morphological studies of small blood vessels and morpho-physiological comparisons.
Rhodin
J A
JA
Department of Anatomy, University of South Florida College of Medicine, Tampa 33612, USA.
Janson
A M
AM
D000818:Animals; D001160:Arterioles / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000648:ultrastructure; D007101:Immersion; D008643:Mesentery / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods; D010477:Perfusion / Q000379:methods; D051381:Rats; D013499:Surface Properties; D016707:Tissue Fixation / Q000379:methods*
Prizhiznennyĭ i élektronno-mikroskopicheskiĭ analiz arteriol pri immersionnom i perfuzionnom sposobakh fiksatsii bryzheĭki krysy.