[Brain lesions in rabbits given an intravenous injection of verotoxin 2 and protection by anti-VT2 antibody].
Magnetic resonance imaging was obtained to determine the effects of VT2 toxemia on the rabbit's CNS. The first lesion was noted at 24 h in the hypothalamic area of all experimental animals. The rabbits accompanied with the brain stem lesion were dead within 6 days. We examined the integrity of cerebrospinal fluid-brain barrier (CBB) using a tracer. The tracer was detected throughout the cytoplasm of the ependymal cell layer covering the third ventricle after intrathecal injection of the tracer, which means a deterioration of CBB. Furthermore, we examined whether anti-VT2 antibody injected intrathecally protects rabbits from brain damage. All the rabbits survived when they were given an intrathecal injection of rabbit anti-VT2 antibody 2h before the intravenous injection of VT2.
Fujii
J
J
Department of Microbiology, School of Medicine, University of Occupational and Environmental Health.
Yoshida
S
S
D000818:Animals; D000992:Antitoxins / Q000008:administration & dosage*; D001427:Bacterial Toxins / Q000633:toxicity*; D001921:Brain / Q000473:pathology; D001927:Brain Diseases / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D004927:Escherichia coli Infections; D019453:Escherichia coli O157; D007278:Injections, Spinal; D008297:Male; D011817:Rabbits; D022641:Shiga Toxin 2
D000992:Antitoxins; D001427:Bacterial Toxins; D022641:Shiga Toxin 2