The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance.
The kinetics of bond formation between spherical beads coated with CD48 and CD2-derivatized surfaces was studied with a flow chamber. For a given shear rate, the binding frequency was exquisitively sensitive to the particle velocity. Flow equations were used to derive the particle-to-surface distance from the velocity, thus yielding a relationship between this distance and the binding rate. Numerical values of the binding site densities allowed absolute determination of the rate of association between two individual molecules as a function of the distance between attachment points. In our model, this rate was about 0.03 s-1 at 10 nm separation, and it was inversely proportional to the cube of the distance.
Pierres
A
A
Laboratoire d'Immunologie, INSERM U 387, Hôpital de Sainte-Marguerite, Marseille, France.
Benoliel
A M
AM
Bongrand
P
P
van der Merwe
P A
PA
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D018801:CD2 Antigens / Q000378:metabolism*; D000071178:CD48 Antigen; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D008863:Microspheres; D011485:Protein Binding; D051381:Rats
D015703:Antigens, CD; D018801:CD2 Antigens; D000071178:CD48 Antigen; C000604067:Cd48 protein, rat; D015815:Cell Adhesion Molecules; D008024:Ligands