[Anterior chamber inflammation after the injection of endothelin-1 into the vitreous and the effect of an anti-prostaglandin agent].
We measured the time course of aqueous protein concentration (APC) with a laser flare-cell meter after the injection of endothelin-1 (ET-1) into the vitreous cavity of pigmented rabbits and investigated the influence of pre- or post-treatment with an anti-prostaglandin agent on these effects of ET-1. Injection of ET-1 significantly increased APC in a dose-dependent fashion. After 10(-5)M ET-1 injection, APC reached maximum at 4 hours after treatment and returned to the normal level 24 hours after the injection. On the other hand, the 10(-4)M ET-1 model displayed a bi-phase time course, with a peak value observed at 4 approximately 8 hours and 48 hours post-treatment, and APC did not return to normal even 7 days after treatment. Treatment with anti-prostaglandin agents before and after the injection blocked APC increase completely in the 10(-5)M ET-1 model, and partially in the 10(-4)M ET-1 model. These results indicate that ET-1 effects on APC are at least partially mediated by the cyclooxygenase pathway of the arachidonic acid cascade.
Shoji
N
N
Department of Ophthalmology, Musashino Red Cross Hospital, Tokyo, Japan.
Oshika
T
T
Masuda
K
K
D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004008:Diclofenac / Q000494:pharmacology; D019332:Endothelin-1 / Q000494:pharmacology*; D005260:Female; D007249:Inflammation / Q000139:chemically induced; D008297:Male; D011448:Prostaglandin Antagonists / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D014822:Vitreous Body / Q000187:drug effects*
D016861:Cyclooxygenase Inhibitors; D019332:Endothelin-1; D011448:Prostaglandin Antagonists; D011506:Proteins; D004008:Diclofenac