Effect of S-adenosyl methionine on muscarinic receptors in young rats.
In the present study we have investigated the effect of a chronic administration of S-Adenosyl Methionine (SAM) on muscarinic receptor subtypes in young rat forebrain, cerebellum, heart and lacrimal gland. Saturation binding experiments were performed using 3H-N-methylscopolamine (3H-NMS) to label the total population of muscarinic receptors in plasma membranes from forebrain, cerebellum, heart and lacrimal gland. 3H-Pirenzepine (3H-Pz) was used to label the M1 subtype in plasma membranes from forebrain. The results obtained in cerebellum, heart and lacrimal gland show no changes in the affinity (Kd) nor in the number of receptors (Bmax) of the treated versus control groups. Saturation experiments in forebrain show an increase in the number of receptors of the treated versus control groups when using 3H-NMS (Bmax 2117 +/- 63 versus 1643 +/- 104 fmol/mg protein) without changes in the affinity. Saturation experiments with 3H-Pz, show an increase in the number of M1 receptors in the treated group with no changes in the affinity (Bmax 421 +/- 16 versus 225 +/- 19 fmol/mg protein). From our results, we conclude that SAM increase the number of receptors in forebrain and this increase is mainly due to changes in the number of M1 receptor subtypes.
Pavia
J
J
Department of Pharmacology, Malaga School of Medicine, Malaga University, Spain.
Martos
F
F
Gonzalez-Correa
J A
JA
Garcia
A J
AJ
Rius
F
F
Laukkonen
S
S
de la Cuesta
F S
FS
D000818:Animals; D002531:Cerebellum / Q000648:ultrastructure; D007700:Kinetics; D007765:Lacrimal Apparatus / Q000648:ultrastructure; D008297:Male; D009206:Myocardium / Q000648:ultrastructure; D016548:Prosencephalon / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000494:pharmacology*
D011976:Receptors, Muscarinic; D012436:S-Adenosylmethionine