title: An approach to the understanding of the nasal early-phase reaction induced by nasal allergen challenge.
abstract: Quantitative determinations of the inflammatory mediators in nasal secretions were performed and correlated with the objective nasal symptoms within 1 h after nasal allergen challenge (NAC). Twenty-six patients with seasonal allergic rhinitis were enrolled outside the pollen season. All measurements were performed before (as a baseline control) and at 1, 5, 10, 30, and 60 min after NAC. This study aimed to clarify the pathogenic mechanism of the early-phase reaction (EPR) by monitoring the evolution of early-phase mediators in nasal secretions and the presence of nasal symptoms during this period. The results showed that, after NAC, the maximal mediator concentration was already reached after 1 min for histamine (124 ng/g), 5 min for tryptase (56 microU/g), and 5-10 min for leukotriene C4 (40 ng/g). Itching and sneezing started as early as 20-30 s, and they were predominant symptoms within 5 min. Rhinorrhea and nasal obstruction started a few minutes after NAC and lasted until more than 1 h after NAC. There was no significant correlation between any single mediator and nasal symptoms during the sampling period. In conclusion, this study demonstrated that during the EPR the presence of nasal symptoms involves a complex mechanism, reflecting the interaction between the mediators released by inflammatory cells, and the receptors on different target organs. When evaluating symptoms during the EPR, one must consider not only the severity of these symptoms but also the time period within which these symptoms occur. For the symptoms of nasal obstruction and rhinorrhea, the early-phase reaction often lasted more than 1 h.
authors.affiliation: Department of Otorhinolaryngology, University Hospital, Free University Brussels, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Smitz
authors.forename: S
authors.initials: S
authors.lastname: Waterschoot
authors.forename: P
authors.initials: P
authors.lastname: Clement
mesh_terms: D000328:Adult; D000485:Allergens / Q000008:administration & dosage* / Q000009:adverse effects*; D053818:Chymases; D005260:Female; D006632:Histamine / Q000378:metabolism; D006801:Humans; D017997:Leukotriene C4 / Q000032:analysis; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism; D015508:Nasal Obstruction / Q000209:etiology; D009299:Nasal Provocation Tests / Q000379:methods*; D011537:Pruritus / Q000209:etiology; D012697:Serine Endopeptidases / Q000032:analysis; D012912:Sneezing; D053802:Tryptases
chemical_list: D000485:Allergens; D017997:Leukotriene C4; D006632:Histamine; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases