title: Physiology of isolated gastric endocrine cells.
abstract: The regulation of gastric acid secretion is achieved in the periphery by interplay between three major gastric endocrine cells: the enterochromaffin-like (ECL) cell, the gastrin or G cell, and the somatostatin or D cell. Regulation of these cells is via stimulatory or inhibitory paracrine, endocrine, and neural pathways. Upregulation of ECL function is determined by activation of CCK-B receptors, by gastrin, and by activation of beta-adrenergic receptors, as well as by acetylcholine in some (10-29%) of the cells. Gastrin and acetylcholine produce typical biphasic calcium signals. Inhibition of ECL cell histamine release and calcium signaling is produced by somatostatin acting at a type 2 receptor, histamine acting at a histamine-3 receptor, and by peptide PYY. Stimulation of ECL cells results in activation of chloride channels, and there is evidence that voltage-dependent calcium channels, along with the receptor-operated calcium channels, also are responsible for elevation of [Ca]i. Depolarization-activated K+ channels presumably restore the potential after depolarization by activation of the chloride channel. The D cell is activated by either gastrin or CCK and appears to be inhibited by acetylcholine and somatostatin. The G cell is activated by acetylcholine and gastrin-releasing peptide (GRP) and is inhibited by somatostatin. The functional integration of these three cell types is the primary determinant of the degree of stimulation of the parietal cell.
authors.affiliation: Department of Medicine, University of California, Los Angeles 90073, USA.
authors.forename: G
authors.initials: G
authors.lastname: Sachs
authors.forename: N
authors.initials: N
authors.lastname: Zeng
authors.forename: C
authors.initials: C
authors.lastname: Prinz
mesh_terms: D000818:Animals; D002469:Cell Separation; D004702:Endocrine Glands / Q000166:cytology* / Q000502:physiology*; D004759:Enterochromaffin Cells / Q000502:physiology; D005744:Gastric Acid / Q000378:metabolism; D006632:Histamine / Q000502:physiology; D006801:Humans; D013270:Stomach / Q000166:cytology* / Q000502:physiology*
chemical_list: D006632:Histamine