title: Genomic analyses of Salmonella enteritidis phage type 4 strains from Austria and phage type 8 strains from the United States.
abstract: Forty illness associated phage-type (PT) 4 and PT 8 strains of Salmonella enteritidis were analyzed by the pulsed-field technique of clamped homogeneous electric fields (CHEF) electrophoresis. Using NotI and XbaI, the 40 strains were subdivided by each enzyme into seven restriction endonuclease digestion profiles (REDP). The 35 PT 4 isolates from Austria were subdivided into six NotI and five XbaI REDP, while the five PT 8 isolates from the United States displayed a single NotI and two XbaI REDP. When highly-concentrated, uncleaved genomic DNA was subjected to CHEF electrophoresis, plasmid DNA in the size range of 350 kb relative to a linear DNA standard was discernible in 38 of the 40 strains. Subsequent isolation and restriction analyses of plasmid DNA from one strain (E40) revealed a single plasmid (pE40; ca. 54 kb) with one XbaI and two NotI cleavage sites that was similar in size to the S. enteritidis virulence plasmid pRQ29. Hybridization of the PE40 probe with S. enteritidis genomic DNAs identified a 54 kb fragment within the XbaI REDP and two fragments, 20 and 34 kb, in NotI REDP of plasmid-positive strains. It was not possible to identify plasmid-specific bands in NotI REDP without hybridization due to comigrating chromosomal and plasmid DNA fragments. Regardless of PT, all 40 S. enteritidis strains showed highly related REDP. The similarity between PT 4 and PT 8 strains as further revealed by Dice similarity coefficients was 90% to 95% for NotI REDP and 79% to 93% for XbaI REDP. These results support the hypothesis that the pandemic observed today is the result of the efficient spread of a single clone, or clusters of closely related clones, of S. enteritidis.
authors.affiliation: Department of Food Microbiology and Toxicology, University of Wisconsin, Madison, USA.
authors.forename: C
authors.initials: C
authors.lastname: Buchrieser
authors.forename: R
authors.initials: R
authors.lastname: Brosch
authors.forename: O
authors.initials: O
authors.lastname: Buchrieser
authors.forename: A
authors.initials: A
authors.lastname: Kristl
authors.forename: J B
authors.initials: JB
authors.lastname: Luchansky
authors.forename: C W
authors.initials: CW
authors.lastname: Kaspar
mesh_terms: D000818:Animals; D001317:Austria / Q000453:epidemiology; D001435:Bacteriophages / Q000472:pathogenicity; D002645:Chickens; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000032:analysis* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012480:Salmonella Infections / Q000453:epidemiology / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000235:genetics*; D012477:Salmonella enteritidis / Q000235:genetics* / Q000821:virology; D014481:United States / Q000453:epidemiology; D014774:Virulence / Q000235:genetics
chemical_list: D004269:DNA, Bacterial