title: Alternative splicing of CREB and CREM mRNAs in an immortalized germ cell line.
abstract: Alternative splicing of CREB (cAMP response element binding protein) and CREM (cAMP response element modulator) mRNAs in separated pachytene spermatocyte, round spermatid, and elongated spermatid fractions and the germ cell-derived immortalized cell line GC-2spd(ts) was studied by reverse transcription polymerase chain reaction (RT-PCR). Both primary germ cells and the GC-2spd(ts) cell line expressed the testis-specific CREB splice variant containing exon W. In the CREB C-E exon region, both primary germ cells and GC-2spd(ts) cells produced RT-PCR products that included exon Y. RT-PCR using CREM primers produced multiple bands in primary germ cells. The truncated CREAM deltaC-G form was found in all the germ cell fractions. The smaller splice forms of CREM were more prominent in the GC-2spd(ts) cells. GC-2spd(ts) cells resembled F9 teratocarcinoma cells more closely than primary germ cells with respect to the relative expression of both CREB and CREM alternative splice products. In Sertoli cells, RT-PCR products of CREB exon lacking W and the product corresponding to CREM delta C-G were most prominent. These data show that the GC-2spd(ts) cell line retains some qualitative characteristics of primary germ cells with respect to alternative splicing of CREB and CREM mRNA.
authors.affiliation: Department of Bio-chemistry and Molecular Biology, University of Texas Houston Medical School 77225, USA.
authors.forename: B M
authors.initials: BM
authors.lastname: Sanborn
authors.forename: J L
authors.initials: JL
authors.lastname: Millan
authors.forename: M L
authors.initials: ML
authors.lastname: Meistrich
authors.forename: L C
authors.initials: LC
authors.lastname: Moore
mesh_terms: D017398:Alternative Splicing / Q000502:physiology*; D000818:Animals; D002461:Cell Line, Transformed / Q000502:physiology; D051786:Cyclic AMP Response Element Modulator; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007668:Kidney / Q000166:cytology; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D012708:Sertoli Cells / Q000166:cytology*; D013094:Spermatozoa / Q000166:cytology*; D018243:Teratocarcinoma; D014407:Tumor Cells, Cultured / Q000502:physiology
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D051786:Cyclic AMP Response Element Modulator