Inhibition by cigarette smoke of lipid peroxidation-induced neurotransmitter release.
Effects of water-soluble substance in cigarette smoke on neurotransmitter release were investigated using nerve terminals (synaptosomes) prepared from rat cerebral cortex. 2,2'-Azobis (2-amidinopropane) dihydrochloride (ABAP), a peroxyl radical-generator, enhanced the depolarization-evoked release of glutamate and aspartate from synaptosomes with concomitant increase in thiobarbituric acid-reactive substances (TBA-RS) levels in membrane lipids of synaptosomes. The trapped smoke-substance attenuated the lipid peroxidation-enhanced release of excitatory amino acids during the depolarization with reduction in TBA-RS, although it failed to affect the basal release of neurotransmitters. These data suggest that cigarette smoke may possess antioxidant properties to reduce oxidation-induced enhancement of transmitter release from nerve terminals.
Kamisaki
Y
Y
Department of Clinical Pharmacology, Faculty of Medicine, Tottori University, Yonago, Japan. kamisaki@grape.med.totori-u.ac.jp
Wada
K
K
Nakamoto
K
K
Kishimoto
Y
Y
Ashida
K
K
Itoh
T
T
D000578:Amidines / Q000494:pharmacology; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005609:Free Radicals / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D000578:Amidines; D005609:Free Radicals; D018377:Neurotransmitter Agents; D017392:Thiobarbituric Acid Reactive Substances; D014028:Tobacco Smoke Pollution; C046728:2,2'-azobis(2-amidinopropane)