Analysis of a cDNA encoding the major vault protein from the electric ray Discopyge ommata.
The major vault protein is the predominant constituent of vaults ubiquitous large cytosolic ribonucleoprotein particles. A cDNA clone encoding the 100-kDa major vault protein (MVP100) was isolated from an electric lobe library of Discopyge ommata. The complete nucleotide sequence was determined. Northern blot analysis revealed a 2.8-kb transcript with a high expression in neural tissue. Southern blot analysis indicates that the electric ray MVP100 is a single copy-gene with at least two introns. The primary structure of major vault proteins characterized in slime mold, ray, rat and human is evolutionary highly conserved.
Herrmann
C
C
Biozentrum, J.W. Goethe-Universität, Zoologisches Institut, Frankfurt am Main, Germany.
Zimmermann
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H
Volknandt
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W
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D012261:Ribonucleoproteins / Q000235:genetics*; D012860:Skates, Fish / Q000235:genetics* / Q000378:metabolism; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein
D018076:DNA, Complementary; D012261:Ribonucleoproteins; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein