Immune localization of prolactin receptor in the mitochondria-rich cells of the euryhaline teleost (Oreochromis mossambicus) gill.
The present work demonstrates, by Western blotting and immunofluorescent staining, the presence and localization of prolactin (PRL) receptor in tilapia (ti) Oreochromis mossambicus gills. Gill epithelial cells that reacted with PRL receptor antibody were found to be labelled concomitantly with Con A, a marker of the apical crypts in mitochondria-rich (MR) cells. No positive staining was observed in pavement cells or mucus cells with PRL receptor antibody. This indicates that PRL receptors are located specifically in the gill MR cells. Further, the tiPRL receptors were found only in the MR cells of seawater-adapted tilapia gills. The effects of salinity and ions on the expression of tiPRL receptors are discussed.
Weng
C F
CF
Institute of Zoology, Academia Sinica, Taipei, Taiwan.
Lee
T H
TH
Hwang
P P
PP
D000818:Animals; D015153:Blotting, Western; D019084:Fluorescent Antibody Technique, Indirect; D005880:Gills / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D008928:Mitochondria; D011981:Receptors, Prolactin / Q000032:analysis*; D017210:Tilapia / Q000378:metabolism*
D011981:Receptors, Prolactin