Effects of NMDA and its antagonists on ventral horn cholinergic neurons in organotypic roller tube spinal cord cultures.
Neurotoxic effects of excitatory amino acid (EAA) receptor agonist N-methyl-D-aspartic acid (NMDA) and its antagonists on ventral horn cholinergic neurons were studied in organotypic rollertube cultures of spinal cord (OTC-SCs) using biochemical assays of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity, and AChE histochemistry. NMDA exposure decreased ChAT and AChE activity by 83% and 66%, respectively. Cultures treated with NMDA also showed a marked loss of AChE staining in both dorsal and ventral horns and a significant, dose-dependent decrease in the number of ventral horn AChE-positive neurons (VHANs). NMDA treatment primarily resulted in the loss of small VHANs (< 300 microns2). VHANs with a size and distribution typical of alpha-motoneurons were relatively well preserved. The effects of NMDA on OTC-SCs appeared to be independent of the age of the cultures. The NMDA antagonist DL-AP5 completely prevented the NMDA-induced loss of ChAT activity, but only attenuated the effect of NMDA on ChE activity. The antagonists DL-AP5, D-AP5 and MK-801, used alone, caused significant loss and/or shrinkage of VHANs. These effects appeared to be distinct from the NMDA mediated toxicity. The results indicate that NMDA and its antagonists exert powerful toxic effects on ventral horn cholinergic neurons. The large cholinergic alpha-motoneurons, however, appear to be relatively immune to these toxic effects.
Delfs
J R
JR
Laboratory for Neurodegenerative and Aging Research, New England Deaconess Hospital, Boston, MA, USA.
Saroff
D M
DM
Nishida
Y
Y
Friend
J
J
Geula
C
C
D000110:Acetylcholinesterase / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000870:Anterior Horn Cells / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002799:Cholinergic Fibers / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology / Q000633:toxicity; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors* / Q000187:drug effects* / Q000633:toxicity; D009474:Neurons / Q000187:drug effects*; D009924:Organ Culture Techniques; D051381:Rats
D018690:Excitatory Amino Acid Agonists; D016202:N-Methylaspartate; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase