title: Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization.
abstract: In order to increase the discrimination of single nucleotide polymorphisms in DNA hybridization, artificial mismatches are inserted into probe oligonucleotides using the base analog 3-nitropyrrole. Differences in thermal stability (delta Tm) between hybrids formed with normal and single-nucleotide-variant DNA targets are increased by as much as 200% over conventional hybridization, and are strongly dependent upon the spacing between mismatches. The increased specificity is demonstrated by hybridization analysis and allele-specific amplification within the HLA-DRB locus.
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison 53706, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Guo
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.forename: L M
authors.initials: LM
authors.lastname: Smith
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001709:Biotechnology; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004355:Drug Stability; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011758:Pyrroles / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D017931:DNA Primers; D006684:HLA-DR Antigens; D015345:Oligonucleotide Probes; D011758:Pyrroles; C094911:3-nitropyrrole; D004247:DNA