title: The rapeseed mitochondrial gene for subunit 2 of the NADH dehydrogenase complex: a trans-spliced structure is conserved in one of the smallest plant mitochondrial genomes.
abstract: The single-copy gene encoding NADH dehydrogenase subunit 2 (nad2) has been identified in the mitochondrial genome of rapeseed (Brassica napus L.). The rapeseed nad2 gene has the same gene organization in Oenothera and wheat: it consists of five exons located in two loci encoding the two first exons and the last three exons respectively. All exons are separated by group-II introns. A trans-splicing event is required to join exons B and C. Putative splicing intermediates were identified by transcriptional analysis of the nad2 gene. The complexity of organization of the nad2 gene is completely conserved even in one of the smallest mitochondrial genomes of higher plants.
authors.affiliation: Laboratory of Plant Genecology, Hokkaido National Agricultural Experiment Station, Hitsuji-ga-oka, Toyohira-ku, Sapporo 062, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Handa
authors.forename: R
authors.initials: R
authors.lastname: Mizobuchi-Fukuoka
authors.forename: W
authors.initials: W
authors.lastname: Pinyarat
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D017124:Conserved Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D017343:Genes, Plant; D018745:Genome, Plant; D008969:Molecular Sequence Data; D009243:NAD / Q000235:genetics*; D009245:NADH Dehydrogenase / Q000235:genetics*; D017393:RNA Editing; D012326:RNA Splicing; D012639:Seeds / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D004272:DNA, Mitochondrial; D009243:NAD; D009245:NADH Dehydrogenase