Structure-affinity relationships for the binding of actinomycin D to DNA.
Molecular models of the complexes between actinomycin D and 14 different DNA hexamers were built based on the X-ray crystal structure of the actinomycin-d(GAAGCTTC)2 complex. The DNA sequences included the canonical GpC binding step flanked by different base pairs, nonclassical binding sites such as GpG and GpT, and sites containing 2,6-diamino-purine. A good correlation was found between the intermolecular interaction energies calculated for the refined complexes and the relative preferences of actinomycin binding to standard and modified DNA. A detailed energy decomposition into van der Waals and electrostatic components for the interactions between the DNA base pairs and either the chromophore or the peptidic part of the antibiotic was performed for each complex. The resulting energy matrix was then subjected to principal component analysis, which showed that actinomycin D discriminates among different DNA sequences by an interplay of hydrogen bonding and stacking interactions. The structure-affinity relationships for this important antitumor drug are thus rationalized and may be used to advantage in design of novel sequence-specific DNA-binding agents.
Gallego
J
J
Department of Physiology and Pharmacology, University of Alcalá, Madrid, Spain.
Ortiz
A R
AR
de Pascual-Teresa
B
B
Gago
F
F
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism*; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D003609:Dactinomycin / Q000737:chemistry* / Q000378:metabolism*; D015195:Drug Design; D006860:Hydrogen Bonding; D066298:In Vitro Techniques; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011487:Protein Conformation; D012984:Software; D055672:Static Electricity; D013816:Thermodynamics
D000900:Anti-Bacterial Agents; D009838:Oligodeoxyribonucleotides; D003609:Dactinomycin; D004247:DNA