Protection against generalized autoimmunity of the nervous system (GANS), a novel animal model with combined features of EAE, EAN and EAU by a recombinant HIV-1 Tat37-72 peptide-based multiple T cell epitope vaccine.
A new model of multi-compartment auto-immune disease has been established to analyze the effects of polyvalent recombinant peptide vaccines. A synthetic gene encoding major pathogenic determinants for Lewis rats of guinea pig myelin basic protein (MBP68-84), bovine interphotoreceptor retinoid binding protein (IRBP1169-1191), and bovine P2 protein (P2,53-78) was used to induce Generalized Autoimmunity of the Nervous System (GANS), which is characterized by development of auto-immune infiltration of the brain and spinal cord, the eyes, the pineal organ and the peripheral nerves. Thus, this model integrates the prominent features of three auto-immune diseases: experimental autoimmune encephalomyelitis (EAE), neuritis (EAN) and uveitis (EAU). In this study, GANS was used to study the effect of HIV-1 Tat37-72 targeting peptide on vaccination by recombinant polyvalent T cell auto-antigen vaccine. Depending on the route of administration, the recombinant vaccine effectively protects against the development of auto-immune nervous system inflammation.
Schluesener
H J
HJ
Institute of Brain Research, University of Tübingen, Germany.
D000818:Animals; D015551:Autoimmunity / Q000276:immunology*; D001483:Base Sequence; D004279:DNA, Viral; D004195:Disease Models, Animal; D004679:Encephalomyelitis / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015696:Gene Products, tat / Q000235:genetics / Q000276:immunology*; D006168:Guinea Pigs; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D009422:Nervous System Diseases / Q000276:immunology*; D009443:Neuritis / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014605:Uveitis / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D054322:tat Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D018984:Epitopes, T-Lymphocyte; D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D054322:tat Gene Products, Human Immunodeficiency Virus