Determination of creatinine and other uremic toxins in human blood sera with micellar electrokinetic capillary electrophoresis.
We have been interested in the clinical use of capillary electrophoresis (CE) to monitor low-molecular-mass uremic toxins in body fluids. Creatinine, an important clinical marker for renal failure, is zwitterionic over a fairly wide pH range (pH 5-9) and can not be resolved from neutral components using free solution CE under these conditions. We report here a micellar electrokinetic capillary chromatography method using an sodium dodecyl sulfate-borate buffer system at pH 9.0 to determine creatinine levels in human serum. This method, performed on deproteinized sera, is also suitable for determining multiple ionic components. Moreover, this method compares favorably with an enzymatic method for creatinine performed in a clinical laboratory and thus appears to be a promising method in terms of potential clinical use.
Tran
T C
TC
Department of Chemistry, Bucknell University, Lewisburg, PA 17837, USA.
Huq
T A
TA
Kantes
H L
HL
Crane
J N
JN
Strein
T G
TG
D003404:Creatinine / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D014118:Toxins, Biological / Q000097:blood*; D014511:Uremia / Q000097:blood*
D008823:Micelles; D014118:Toxins, Biological; D003404:Creatinine