title: Correlation of ATP/citrate lyase activity with lipid accumulation in developing seeds of Brassica napus L.
abstract: The temporal distribution of ATP/citrate lyase (ACL) activity in developing seeds of Brassica napus L. closely paralleled both that of acetyl-CoA carboxylase and the overall rate of lipid biosynthesis. Maximum ACL activities (250 nmol acetyl-CoA formed min-1.g fresh seed) were recorded between 35 to 42 d after pollination and, if the in vitro data could be extrapolated to the situation in vivo, could account for half of the acetyl-CoA required for the measured rate of fatty acid biosynthesis during seed development. The enzyme appeared to be localized in a subcellular compartment, which was clearly separated from mitochondria on a sucrose gradient and by differential centrifugation, and which corresponded to the chloroplast organelle.
authors.affiliation: Department of Biological Sciences, University of Hull, United Kingdom.
authors.forename: C
authors.initials: C
authors.lastname: Ratledge
authors.forename: M D
authors.initials: MD
authors.lastname: Bowater
authors.forename: P N
authors.initials: PN
authors.lastname: Taylor
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D001937:Brassica / Q000201:enzymology*; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002736:Chloroplasts / Q000201:enzymology / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D005649:Fumarate Hydratase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis; D008291:Malate Dehydrogenase / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D012639:Seeds / Q000201:enzymology / Q000378:metabolism
chemical_list: D000085:Acetates; D008055:Lipids; D010938:Plant Oils; D019343:Citric Acid; D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); D005954:Glucosephosphate Dehydrogenase; D001275:ATP Citrate (pro-S)-Lyase; D005649:Fumarate Hydratase; D000103:Acetyl-CoA Carboxylase