title: Development of a high-performance liquid chromatographic-atmospheric pressure chemical ionization-tandem mass spectrometric assay for beta-tigogenin cellobioside in human serum.
abstract: A specific high-performance liquid chromatographic-atmospheric pressure chemical ionization tandem mass spectrometric assay for the quantitative determination of beta-tigogenin cellobioside in human serum is described. Serum cleanup and acetylation of the analyte were required to achieve the desired lower limit of quantification, 10 ng/ml. The precision of the assay was better than 13% over a serum concentration range of 10-500 ng/ml.
authors.affiliation: Central Research Division, Pfizer Inc., Groton, CT 06340, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Avery
authors.forename: H G
authors.initials: HG
authors.lastname: Fouda
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D012503:Saponins / Q000097:blood*
chemical_list: D012503:Saponins; C080606:tiqueside