Old age does not affect shortening velocity or content of contractile and cytoskeletal proteins in the rat detrusor smooth muscle.
The influence of old age on mechanical properties of the urinary bladder was investigated using smooth muscle strips from urinary bladders of control (14-16 weeks) and old-age (104 weeks) female Sprague-Dawley rats. Bladder weight of the aged rats had increased by about 30%. The maximal shortening velocity and stiffness in skinned activated urinary bladder fibers from old animals were unchanged compared to controls. The relative content of intermediate filament proteins to actin and the relative content of myosin to actin was unchanged. The concentration of myosin was unchanged (about 6.5 microg/mg wet weight). The results suggest that old age is not associated with pronounced changes in the cellular contractile and cytoskeletal proteins or in the mechanical properties of the contractile machinery. The age-related changes in mechanical properties previously reported for intact smooth muscle from urinary bladder are most likely due to alterations in the activation systems.
Sjuve
R
R
Department of Physiology and Neuroscience, Lund University, Sweden.
Uvelius
B
B
Arner
A
A
D000199:Actins / Q000032:analysis; D000375:Aging / Q000502:physiology*; D000818:Animals; D003285:Contractile Proteins / Q000378:metabolism / Q000502:physiology*; D003598:Cytoskeletal Proteins / Q000378:metabolism / Q000502:physiology*; D005260:Female; D007381:Intermediate Filament Proteins / Q000032:analysis; D009130:Muscle, Smooth / Q000737:chemistry / Q000502:physiology*; D009218:Myosins / Q000032:analysis; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D001743:Urinary Bladder / Q000737:chemistry / Q000502:physiology*
D000199:Actins; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D007381:Intermediate Filament Proteins; D009218:Myosins