Cross-competition for TATA-binding protein between TATA boxes of the selenocysteine tRNA[Ser]Sec promoter and RNA polymerase II promoters.
In this study we show that the TATA-binding protein (TBP) interacts with the selenocysteine tRNA[Ser]Sec TATA element in a fashion analogous to the TBP-TATA interaction in RNA polymerase (Pol) II-transcribed genes even though the gene is transcribed by Pol III. Recombinant TBPs expressed in Escherichia coli bound to the tRNA[Ser]Sec TATA element. A factor was detected in Xenopus oocyte extracts which contain TBP and bind to the TATA boxes of the tRNA[Ser]Sec gene and various class II genes. Transcription of the microinjected tRNA[Ser]Sec gene was inhibited in Xenopus oocytes by coinjection with the TATA box of the adenovirus major late promoter (AdMLP). Transcription of a 5S gene was not affected under these conditions. These results suggest that the tRNA[Ser]Sec gene recruits TBP in a manner similar to that of TATA-dependent Pol II-transcribed genes and differently from that of Pol III-transcribed genes lacking a TATA box.
Park
J M
JM
Laboratory of Molecular Genetics, Seoul National University, Korea.
Hatfield
D L
DL
Lee
B J
BJ
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D009865:Oocytes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012319:RNA Polymerase II / Q000235:genetics*; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016385:TATA Box; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
D004268:DNA-Binding Proteins; D012345:RNA, Transfer, Amino Acid-Specific; D011994:Recombinant Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; C036928:tRNA, selenocysteine-; D012319:RNA Polymerase II