title: Characterisation of antibiotic moenomycin A interaction with phospholipid model membranes.
abstract: Using a combination of physico-chemical techniques (MAS NMR, DSC, freeze-fracture electron microscopy, molecular modelling) the antibiotic moenomycin A was found to be anchored by its hydrophobic chain into multilamellar POPC membranes. The lamellar phase structure of the modified membrane is retained, while moenomycin A in water at different concentrations does not form any other but isotropic phase structures. The mobility of POPC molecule segments is reduced with increasing moenomycin A concentrations. Freeze-fracture electron microscopy images show ripple like structures for low moenomycin A concentrations, which are rare for high concentrations. A sugar-group network of the antibiotic seems to cover the whole membrane surface for molar ratios moenomycin A/POPC of 1:2, which is supported by 13C-MAS (Magic Angle Spinning) 31P-NMR, and molecular modelling.
authors.affiliation: Universität Leipzig, Fakultät für Physik und Geowissenschaften, Leipzig, Germany. volke@rz.uni-leipzig.de
authors.forename: F
authors.initials: F
authors.lastname: Volke
authors.forename: R
authors.initials: R
authors.lastname: Waschipky
authors.forename: A
authors.initials: A
authors.lastname: Pampel
authors.forename: A
authors.initials: A
authors.lastname: Donnerstag
authors.forename: G
authors.initials: G
authors.lastname: Lantzsch
authors.forename: H
authors.initials: H
authors.lastname: Pfeiffer
authors.forename: W
authors.initials: W
authors.lastname: Richter
authors.forename: G
authors.initials: G
authors.lastname: Klose
authors.forename: P
authors.initials: P
authors.lastname: Welzel
mesh_terms: D001455:Bambermycins / Q000737:chemistry* / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008854:Microscopy, Electron; D008958:Models, Molecular; D015394:Molecular Structure; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
chemical_list: D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D001455:Bambermycins