title: Preoperative fine needle aspiration from human breast cancer is a valuable sampling material for progesterone receptor and cytometric DNA analysis.
abstract: In a breast cancer series (n = 54), preoperative fine needle aspiration (FNA) was compared with biopsy at primary surgery as a source of material for the determination of progesterone receptor (PgR) content by enzyme immuno assay. The respective results manifested a strong correlation (r(s) = 0.82). The fact that PgR content was usually higher in FNA samples than in the corresponding biopsy samples and the finding that 11% of the tumours were PgR positive in FNA but PgR negative in the corresponding biopsy samples suggest a greater proportion of malignant cells to be obtained with FNA than in surgical biopsy. In another breast cancer series (n = 50), corresponding comparisons for DNA flow cytometry showed concordance in ploidy status (diploid vs. non-diploid) in 84% of cases and a strong correlation in S-phase fraction values (r(s) = 0.70). At DNA image cytometry, concordant results (Auer I + II vs. Auer III + IV) were obtained in 87% of the cases. To sum up, FNA seems to be a useful sampling technique for PgR determination and DNA cytometry.
authors.affiliation: Department of Oncology, University Hospital, Lund, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Fernö
authors.forename: B
authors.initials: B
authors.lastname: Baldetorp
authors.forename: G
authors.initials: G
authors.lastname: Fallenius
authors.forename: I
authors.initials: I
authors.lastname: Idvall
authors.forename: U
authors.initials: U
authors.lastname: Johansson
authors.forename: D
authors.initials: D
authors.lastname: Killander
mesh_terms: D000782:Aneuploidy; D001706:Biopsy; D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000175:diagnosis*; D004273:DNA, Neoplasm / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D011980:Receptors, Progesterone / Q000032:analysis*; D016196:S Phase
chemical_list: D004273:DNA, Neoplasm; D011980:Receptors, Progesterone