Four-hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique.
A protocol for routine 4-hour microwave tissue processing of clinical or other samples for electron microscopy was developed. Specimens are processed by using a temperature-restrictive probe that can be set to automatically cycle the magnetron to maintain any designated temperature restriction (temperature maximum). In addition, specimen processing during fixation is performed in 1.7-ml microcentrifuge tubes followed by subsequent processing in flow-through baskets. Quality control is made possible during each step through the addition of an RS232 port to the microwave, allowing direct connection of the microwave oven to any personal computer. The software provided with the temperature probe enables the user to monitor time and temperature on a real-time basis. Tissue specimens, goat placenta, mouse liver, mouse kidney, and deer esophagus were processed by conventional and microwave techniques in this study. In all instances, the results for the microwave-processed samples were equal to or better than those achieved by routine processing techniques.
Giberson
R T
RT
Department of Biological Sciences, California State University, Chico 95929, USA.
Demaree
R S
RS
Nordhausen
R W
RW
D000257:Adenoviridae Infections / Q000473:pathology / Q000662:veterinary; D000818:Animals; D003670:Deer; D004947:Esophagus / Q000648:ultrastructure*; D005260:Female; D006041:Goats; D007668:Kidney / Q000648:ultrastructure*; D008099:Liver / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods / Q000592:standards; D008872:Microwaves; D010920:Placenta / Q000648:ultrastructure*; D011247:Pregnancy; D011786:Quality Control