compound A induces sister chromatid exchanges in Chinese hamster ovary cells.
BACKGROUND
Compound A [CF2 = C(CF3)OCH2F], a degradation product of sevoflurane [(CF3)2CHOCH2F], is a vinyl ether and may be an alkylating agent. Thus it is a potential genotoxin.


METHODS
The capacity of compound A to produce sister chromatid exchanges was measured in Chinese hamster ovary cells with and without metabolic activation. Concentrations of 11 to 468 ppm compound A were applied for 2 h, the Chinese hamster ovary cells were incubated for a further 34 h in the presence of bromodeoxyuridine, and then colcemid was added to produce arrest in metaphase. Coded slides of cells were examined blindly, and 50 chromosome spreads were counted for each test concentration.


RESULTS
The lowest concentration of compound A applied without metabolic activator (27 ppm) significantly increased (P < 0.001) sister chromatid exchanges, and increasing concentrations of compound A increased the incidence of exchanges. Metabolic activation did not increase the incidence of exchanges.


CONCLUSIONS
Compound A increases sister chromatid exchanges at concentrations (27 ppm) obtained in low-flow systems when sevoflurane is used at concentrations approximating minimum alveolar concentration.
Eger
E I
EI
Department of Anesthesiology, University of California, San Francisco 94143-0464, USA.
Laster
M J
MJ
Winegar
R
R
Han
C
C
Gong
D
D
D018685:Anesthetics, Inhalation / Q000633:toxicity*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004987:Ethers / Q000633:toxicity*; D006845:Hydrocarbons, Fluorinated / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D018685:Anesthetics, Inhalation; D004987:Ethers; D006845:Hydrocarbons, Fluorinated; D009153:Mutagens; C078742:fluoromethyl 2,2-difluoro-1-(trifluoromethyl)vinyl ether