title: Study of the DNA content by flow cytometry and proliferation in 281 brain tumors.
abstract: In this paper we investigate the distribution of DNA ploidy as well as proliferation rate (S phase of the cell cycle Ki-67 staining) in 281 tumors of the central and peripheral nervous system (87 meningiomas, 75 astrocytomas, 44 nerve sheath tumors, 25 brain metastases, 18 pituitary adenomas, 17 ependymomas, 12 oligodendrogliomas and 3 medulloblastomas) and their correlation with the histopathological grade. Considering all 281 tumors, aneuploidy is the most frequent finding present: 52%. This percentage increases with malignancy: 69% of malignant tumors are aneuploidy. Levels of aneuploidy decrease from brain metastases to pituitary adenoma (92% in brain metastases, 83% in oligodendrogliomas, 59% in nerve sheath tumors, 47% in ependymomas, 40% in astrocytomas, 35% in meningiomas and 33% in pituitary adenomas), but aneuploidy is also found in many benign tumors. With respect to proliferation rate of tumors, S phase above 20% were recorded in the more malignant tumors: brain metastases, oligodendrogliomas, high-grade ependymomas, high grade astrocytomas, and in atypical and malignant meningiomas, but this parameter is not able to distinguish between low and high grade tumors. However, Ki-67 reactivity was equivalent in all histologies with significant differences between low and high grade tumors.
authors.affiliation: Department of Immunology, Universidad Autonoma, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: García
authors.forename: A
authors.initials: A
authors.lastname: Bueno
authors.forename: S
authors.initials: S
authors.lastname: Castañon
authors.forename: P
authors.initials: P
authors.lastname: Ruiz-Barnes
authors.forename: J
authors.initials: J
authors.lastname: Maria de Campos
authors.forename: E
authors.initials: E
authors.lastname: Kusak
authors.forename: J R
authors.initials: JR
authors.lastname: Fortes
authors.forename: F
authors.initials: F
authors.lastname: Ortiz
authors.forename: J L
authors.initials: JL
authors.lastname: Sarasa
mesh_terms: D000236:Adenoma / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001932:Brain Neoplasms / Q000235:genetics* / Q000556:secondary; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000235:genetics*; D005434:Flow Cytometry; D005910:Glioma / Q000235:genetics; D006801:Humans; D008577:Meningeal Neoplasms / Q000235:genetics; D008579:Meningioma / Q000235:genetics; D008875:Middle Aged; D018317:Nerve Sheath Neoplasms / Q000235:genetics; D010911:Pituitary Neoplasms / Q000235:genetics; D011003:Ploidies; D011237:Predictive Value of Tests; D011379:Prognosis
chemical_list: D004273:DNA, Neoplasm