Identification of an Arabidopsis locus required for resistance to turnip crinkle virus.
Inoculation of turnip crinkle virus (TCV) into a (TCV)-resistant line of Arabidopsis thaliana, Di-17, results in the development of a hypersensitive response (HR) on the inoculated leaves. In contrast, an HR does not occur when leaves of the TCV-susceptible Di-3 line or the susceptible ecotypes Columbia (Col-0), or Landsberg erecta (Ler) are inoculated. Genetic analysis of progeny from crosses between Di-17 and either Di-3, Col-0 or Ler demonstrates that the development of an HR is regulated by a single dominant nuclear locus, herein designated HRT. Using progeny from a Di-17 x Col-0 cross, HRT was mapped to chromosome 5, where it is tightly linked to the DFR locus. We also demonstrate that a variety of resistance-associated phenomena, including the TCV-induced accumulation of salicylic acid, camalexin and autofluorescent cell-wall material, correlate with the HR, suggesting the possibility that HRT is required for their activation.
Dempsey
D A
DA
Waksman Institute, Piscataway, NJ, USA.
Pathirana
M S
MS
Wobbe
K K
KK
Klessig
D F
DF
D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology / Q000821:virology; D019184:Carmovirus / Q000254:growth & development*; D002473:Cell Wall / Q000737:chemistry; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017343:Genes, Plant / Q000502:physiology*; D007211:Indoles / Q000032:analysis; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000032:analysis; D012459:Salicylates / Q000032:analysis; D020156:Salicylic Acid; D013844:Thiazoles / Q000032:analysis; D000094162:Phytoalexins
D007211:Indoles; D010940:Plant Proteins; D012459:Salicylates; D020156:Salicylic Acid; D013844:Thiazoles; C102405:camalexin; D000094162:Phytoalexins