Regulation by fatty acids of angiotensinogen gene expression in preadipose cells.
Adipocytes represent an important source of angiotensiongen (AT). Angiotensin II (A-II) stimulates in vitro and in vivo the formation and release of prostacyclin which acts as a potent adipogenic signal in triggering the terminal differentiation of preadipocytes into adipocytes [Darimont, Vassaux, Gaillard. Ailhaud and Négrel (1994) Int. J. Obes. 18, 783-788]. Since fatty acids have been reported to activate in preadipose cells the expression of various differentiation-dependent genes, the role of fatty acids in the regulation of AT gene expression was investigated. Long-chain natural and non-metabolized fatty acids as well as peroxisome proliferators behave as activators of AT gene expression. Accumulation of AT mRNA parallels that of the adipocyte fatty acid-binding protein gene and is primarily due to transcriptional activation of the AT gene. AT mRNA decreases after fatty acid removal (half-life approx. 8 h). Secretion of AT is also observed but appears mainly as a late differentiation-dependent phenomenon. Thus the AT gene appears to be a fatty acid-responsive gene; this regulation provides a potential link between the flux of fatty acids and the potential of adipose tissue to produce AT and possibly A-II.
Safonova
I
I
Centre de Biochimie du CNRS (UMR 6543), Université de Nice-Sophia Antipolis, France.
Aubert
J
J
Negrel
R
R
Ailhaud
G
G
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000808:Angiotensinogen / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation; D002460:Cell Line; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006207:Half-Life; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D018996:Myelin P2 Protein / Q000096:biosynthesis / Q000187:drug effects; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014158:Transcription, Genetic
D002352:Carrier Proteins; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D047908:Intracellular Signaling Peptides and Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C052998:peroxisome proliferator-binding protein, rat; D000808:Angiotensinogen