Comparison of polymerase chain reaction and pulsed-field gel electrophoresis for the epidemiological typing of Campylobacter jejuni.
Seventeen sporadic Campylobacter jejuni enteritis cases occurred in Taichung City, Taiwan between July 1995 and September 1995. Pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus (ERIC-1) primed polymerase chain reaction (PCR) techniques were compared for the epidemiological typing of the 17 C. jejuni isolates. Fourteen distinct PFGE fingerprint patterns were observed. Fifteen distinct PCR fingerprint patterns were demonstrated. Two clusters of isolates (isolates 5 and 6; isolates 10, 11 respectively) were found to be genetically indistinguishable by both methods. In conclusion, we consider that PFGE is a highly reproducible method for determining the relatedness among the C. jejuni isolates in this study, although their limited numbers of restriction fragments may reduce the discriminatory power. Although less reproducible than PFGE typing, ERIC-1 primed PCR can be used as a simple and rapid tool to discriminate different strains of C. jejuni.
Shi
Z Y
ZY
Section of Infectious Diseases, Taichung Veterans General Hospital, Taiwan, ROC.
Liu
P Y
PY
Lau
Y J
YJ
Lin
Y H
YH
Hu
B S
BS
Tsai
H N
HN
D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D004751:Enteritis / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D017720:Molecular Epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction