Micronucleated cells during the growth of the Ehrlich ascites tumour on spontaneous and by titanocenedichloride-induced conditions.
The micronucleus assay was used to investigate the activity of the Ehrlich-ascites tumour (EAT) of mice to produce chromosomal aberrations under spontaneous and chemically induced conditions. Titanocene dichloride (TDC), a cytostatic drug similar to cis-platinum, was injected in several dosages into the peritoneal cavity of mice carrying the tumour (40, 60 or 80 mg/kg). Our results show that there exists a constant spontaneous level of micronucleated cells (MNC), and that TDC is a potential dose-related inductor of micronuclei (MN). The highest amounts of MN are registered 48 h after application. We discuss the results in context with basic cytokinetic data of our tumour. This leads to the postulation of a dynamic balance of generating and eliminating factors producing the actual level of MNC.
Tarillion
P
P
Institute of Pathology, Leipzig, Germany.
Taubert
G
G
Nenning
H
H
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D005260:Female; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009942:Organometallic Compounds / Q000633:toxicity*; D014025:Titanium / Q000633:toxicity*
D000970:Antineoplastic Agents; D009153:Mutagens; D009942:Organometallic Compounds; D014025:Titanium; C020528:titanocene dichloride