Antisense transcription of a murine FGFR-3 psuedogene during fetal developement.
In a search for new protein tyrosine kinases (PTKs) in early hemopoietic cells, we have identified a sequence closely related to the Fibroblast Growth Factor Receptor (FGFR) family. A cDNA isolated from a mouse embryo library was 89% identical to FGFR-3 in both its coding and 3' untranslated regions. However, the region homologous to exons 5 to 9 of FGFR-3 was missing. In addition, the ORF was interrupted by several stop codons and frame shifts, indicating that this sequence is not functional. These transcripts were therefore copied from a novel FGFR-3 pseudogene, that we called psiFGFR-3. Partial analysis of this gene showed the absence of introns, which is a characteristic feature of a processed pseudogene. psiFGFR-3 gene was localized on Chromosome 1H4-6. Its transcription was shown to be antisense and its expression was restricted to fetal tissues. These results indicate that psiFGFR-3 has been inserted in Chromosome 1 in antisense orientation close to a heterologous promoter.
Weil
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Leukaemia Foundation of Queensland Daikyo Research Unit, Queensland Institute of Medical Research, Herston, Australia. weil@infobiogen.fr
Power
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Webb
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D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000378:metabolism; D005346:Fibroblast Growth Factors; D018507:Gene Expression Regulation, Developmental; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000737:chemistry; D011544:Pseudogenes / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics*; D051498:Receptor, Fibroblast Growth Factor, Type 3; D017468:Receptors, Fibroblast Growth Factor / Q000737:chemistry / Q000235:genetics*; D017421:Sequence Analysis; D017384:Sequence Deletion / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D016372:RNA, Antisense; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors; C496366:Fgfr3 protein, mouse; D011505:Protein-Tyrosine Kinases; D051498:Receptor, Fibroblast Growth Factor, Type 3