Involvement of endothelin in graft-versus-host disease after rat small bowel transplantation.
Endothelin (ET-1) expression was evaluated by radioimmunoassay in both plasma and various tissue specimens serially obtained from LBN-F1 recipients of LEW heterotopic small bowel allografts. The recipients showed graft-versus-host disease (GVHD), which histologically became apparent on postoperative day (POD) 13. The ET-1 levels peaked on POD 9 in the kidney, lung, and host intestine at 51.0 +/- 21.1, 90.9 +/- 59.6, and 25.4 +/- 11.8 pg/g wet, respectively, and peaked on POD 11 in the plasma at 7.7 +/- 3.2 pg/ml; thereafter, they decreased to basal levels in both the plasma and tissue specimens on POD 13. An immunohistochemical study of these organs showed a corresponding increase in ET-1 staining in both the endothelial and epithelial cells on PODs 5 and 9, and a reduction in staining on POD 13. In conclusion, ET-1 was found to be increasingly released from the target cells of GVHD before any histological changes became apparent, thus suggesting the pathophysiological involvement of ET-1 in intestinal GVHD.
Hiroyasu
S
S
First Department of Surgery, University of Ryukyu, School of Medicine, Okinawa, Japan.
Shiraishi
M
M
Kusano
T
T
Muto
Y
Y
D000818:Animals; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000097:blood; D006086:Graft vs Host Disease / Q000473:pathology / Q000503:physiopathology*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000473:pathology / Q000637:transplantation*; D007421:Intestine, Small / Q000473:pathology / Q000637:transplantation*; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013997:Time Factors; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous / Q000276:immunology* / Q000473:pathology
D019332:Endothelin-1