[The paradoxical effect of ethanol on the protein status of a primary chick embryo myoblast culture].
Effects of 0.1-10% ethanol on creatine kinase activity and water-soluble protein amount were studied in extracts of 2 day-old culture of chick embryo myoblasts. Low concentrations of the toxicant stimulated the total creatine kinase activity of the culture whereas middle concentrations exerted no influence, and the highest concentration (10%) oppressed the enzymatic activity. The increase of protein content in extracts was observed when ethanol concentration in the incubation medium was 0.1%. Within the range of 1-5% ethanol the protein content sharply decreased at 10% concentration it did not differs from the control value. The treatment of 5 day-old culture with 1-5% ethanol did not influence either creatine kinase activity or protein content in myoblasts. The obtained data are discussed in the context of chemical hormesis scheme and in terms of unmasked changes involvement in the adaptive reactions of the cell system.
Matiushichev
V B
VB
Sokolov
I N
IN
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009124:Muscle Proteins / Q000096:biosynthesis / Q000187:drug effects*; D013997:Time Factors
D009124:Muscle Proteins; D000431:Ethanol; D003402:Creatine Kinase
Paradoksal'noe vliianie étanola na belkovoĭ status pervichnoĭ kul'tury mioblastov kurinogo émbriona.