title: Hyaluronic acid inhibits interleukin-1-induced superoxide anion in bovine chondrocytes.
abstract: OBJECTIVE
  To examine the effect of hyaluronic acid (HA) on the induction of superoxide anion by IL-1 in chondrocytes.
  
  
  METHODS
  Bovine articular chondrocytes were treated with different concentrations of IL-1. A chemiluminescent probe (L-012) was added to the medium and chemiluminescence detection was used to measure super oxide anion.
  
  
  RESULTS
  IL-1 caused induction of superoxide anions in a dose-dependent manner. HA (10-100 micrograms/ml) countered superoxide induction caused by 20 ng/ml of IL-1.
  
  
  CONCLUSIONS
  HA can afford protection against cartilage degradation, probably acting as a free-radical scavenger.
authors.affiliation: Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fukuda
authors.forename: M
authors.initials: M
authors.lastname: Takayama
authors.forename: M
authors.initials: M
authors.lastname: Ueno
authors.forename: M
authors.initials: M
authors.lastname: Oh
authors.forename: S
authors.initials: S
authors.lastname: Asada
authors.forename: F
authors.initials: F
authors.lastname: Kumano
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D004353:Drug Evaluation, Preclinical; D006820:Hyaluronic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008163:Luminescent Measurements; D013481:Superoxides / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D013481:Superoxides; D006820:Hyaluronic Acid