title: A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
abstract: The 465,813 base pair sequence corresponding to the 40.1-50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2-46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified a new asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the cis-element.
authors.affiliation: Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: H
authors.initials: H
authors.lastname: Aiba
authors.forename: T
authors.initials: T
authors.lastname: Baba
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Inada
authors.forename: K
authors.initials: K
authors.lastname: Isono
authors.forename: H
authors.initials: H
authors.lastname: Kasai
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Kitakawa
authors.forename: M
authors.initials: M
authors.lastname: Kitagawa
authors.forename: K
authors.initials: K
authors.lastname: Makino
authors.forename: T
authors.initials: T
authors.lastname: Miki
authors.forename: K
authors.initials: K
authors.lastname: Mizobuchi
authors.forename: H
authors.initials: H
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Motomura
authors.forename: S
authors.initials: S
authors.lastname: Nakade
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Nashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Nishio
authors.forename: T
authors.initials: T
authors.lastname: Oshima
authors.forename: N
authors.initials: N
authors.lastname: Saito
authors.forename: G
authors.initials: G
authors.lastname: Sampei
authors.forename: Y
authors.initials: Y
authors.lastname: Seki
authors.forename: T
authors.initials: T
authors.lastname: Horiuchi
mesh_terms: D001287:Attachment Sites, Microbiological; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005810:Multigene Family; D016366:Open Reading Frames; D012348:RNA, Transfer, Asn / Q000235:genetics; D012093:Replicon; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D012348:RNA, Transfer, Asn