title: Levels of Porphyromonas gingivalis Fimbriae and inflammatory cytokines in gingival crevicular fluid from adult human subjects.
abstract: The Porphyromonas gingivalis fimbriae level was examined in the gingival crevicular fluid (GCF) from adult human subjects using an immunoblot assay with a monoclonal antibody. The cytokines, interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-6 and tumor necrosis factor-alpha (TNF-alpha) levels in the GCF were quantified by enzyme-linked immunosorbent assay (ELISA). The reactivity of the GCF samples with the monoclonal antibody against P. gingivalis fimbriae was related to the IL-2 beta, IL-6 and TNF-alpha levels. Moreover, the fimbriae content was associated with the gingival index (GI). In contrast, no significant correlation was seen between the fimbriae content and IL-1 alpha level. These results suggest that there are possible associations between P. gingivalis fimbriae and IL-1 beta, IL-6 and TNF-alpha in the gingival crevicular fluid.
authors.affiliation: Department of Preventive Dentistry, School of Dentistry, Health Sciences University of Hokkaido, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: E
authors.initials: E
authors.lastname: Isogai
authors.forename: H
authors.initials: H
authors.lastname: Miura
authors.forename: I
authors.initials: I
authors.lastname: Ueda
mesh_terms: D000328:Adult; D005260:Female; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D005883:Gingival Crevicular Fluid / Q000378:metabolism* / Q000382:microbiology*; D006801:Humans; D007249:Inflammation; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000378:metabolism / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha