The HLA-A*0201-restricted H-Y antigen contains a posttranslationally modified cysteine that significantly affects T cell recognition.
A peptide recognized by two cytotoxic T cell clones specific for the human minor histocompatibility antigen H-Y and restricted by HLA-A*0201 was identified. This peptide originates from SMCY, as do two other H-Y epitopes, supporting the importance of this protein as a major source of H-Y determinants in mice and humans. In naturally processed peptides, T cells only recognize posttranslationally altered forms of this peptide that have undergone modification of a cysteine residue in the seventh position. One of these modifications involves attachment of a second cysteine residue via a disulfide bond. This modification has profound effects on T cell recognition and also occurs in other class I MHC-associated peptides, supporting its general importance as an immunological determinant.
Meadows
L
L
Department of Chemistry, University of Virginia, Charlottesville 22901, USA.
Wang
W
W
den Haan
J M
JM
Blokland
E
E
Reinhardus
C
C
Drijfhout
J W
JW
Shabanowitz
J
J
Pierce
R
R
Agulnik
A I
AI
Bishop
C E
CE
Hunt
D F
DF
Goulmy
E
E
Engelhard
V H
VH
D000818:Animals; D016477:Artifacts; D002478:Cells, Cultured; D002999:Clone Cells; D003545:Cysteine / Q000235:genetics* / Q000378:metabolism; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D005260:Female; D006182:H-Y Antigen / Q000235:genetics*; D015789:HLA-A2 Antigen / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding / Q000276:immunology; D011499:Protein Processing, Post-Translational / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000939:Epitopes; D006182:H-Y Antigen; D015789:HLA-A2 Antigen; D010455:Peptides; D011948:Receptors, Antigen, T-Cell; D003545:Cysteine