title: Polyamines in growth and dimorphism of Paracoccidioides brasiliensis.
abstract: Putrescine and spermidine were the only polyamines found in Paracoccidioides brasiliensis, a dimorphic fungus pathogenic for humans. Free polyamines (putrescine > spermidine) increased during the first 24 h of yeast growth, with a second peak at 42 h, and also during the first 12 h of mycelium-to-yeast transition (spermidine > putrescine). Conjugated and bound polyamines were also quantified. 1, 4-Diamino-2-butanone decreased free putrescine and spermidine accumulation by inhibiting the activity of ornithine decarboxylase. The increase in free polyamines corresponds to bud emergence in yeast growth and to the mycelium-to-yeast transition of P. brasiliensis.
authors.affiliation: Instituto Venezolano de Investigaciones Científicas (IVIC), Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020A, Venezuela.
authors.forename: G
authors.initials: G
authors.lastname: San-Blas
authors.forename: F
authors.initials: F
authors.lastname: San-Blas
authors.forename: F
authors.initials: F
authors.lastname: Sorais
authors.forename: B
authors.initials: B
authors.lastname: Moreno
authors.forename: J
authors.initials: J
authors.lastname: Ruiz-Herrera
mesh_terms: D065108:Ornithine Decarboxylase Inhibitors; D010228:Paracoccidioides / Q000254:growth & development* / Q000378:metabolism*; D011700:Putrescine / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000494:pharmacology; D013095:Spermidine / Q000096:biosynthesis*
chemical_list: D065108:Ornithine Decarboxylase Inhibitors; C014458:1,4-diaminobutanone; D013095:Spermidine; D011700:Putrescine