title: Spermidine biosynthesis in Saccharomyces cerevisae: polyamine requirement of a null mutant of the SPE3 gene (spermidine synthase).
abstract: The Saccharomyces cerevisiae SPE3 gene, coding for spermidine synthase, was cloned, sequenced, and localized on the right arm of chromosome XVI. The deduced amino acid sequence has a high similarity to mammalian spermidine synthases, and has putative S-adenosylmethionine binding motifs. To investigate the effect of total loss of the SPE3 gene, we constructed a null mutant of this gene, spe3delta, which has no spermidine synthase activity and has an absolute requirement for spermidine or spermine for the growth. This requirement is satisfied by a very low concentration of spermidine (10(-8) M) or a higher concentration of spermine (10(-6) M).
authors.affiliation: Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA.
authors.forename: N
authors.initials: N
authors.lastname: Hamasaki-Katagiri
authors.forename: C W
authors.initials: CW
authors.lastname: Tabor
authors.forename: H
authors.initials: H
authors.lastname: Tabor
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003839:Deoxyadenosines / Q000378:metabolism; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D012436:S-Adenosylmethionine / Q000031:analogs & derivatives / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013095:Spermidine / Q000096:biosynthesis* / Q000378:metabolism; D011436:Spermidine Synthase / Q000235:genetics*; D013872:Thionucleosides / Q000378:metabolism
chemical_list: D003839:Deoxyadenosines; D013872:Thionucleosides; C012702:S-adenosyl-3-methylthiopropylamine; C008500:5'-methylthioadenosine; D012436:S-Adenosylmethionine; D011436:Spermidine Synthase; D013095:Spermidine