Studies on the structure of sperm heads of Eledone cirrhosa by means of CLSM linked to bioimage-oriented devices.
We have used a confocal laser scanning optical microscope imaging device and a bioimage-oriented workstation equipped for augmented reality to study the helical sperm head of the octopus Eledone cirrhosa. This approach allows us to study different complex organisational motifs due to the spatial arrangement of linear helical structures. We consider this helical specimen an enlarged copy of one of the most important biostructures governing cell functioning such as chromatin-DNA. Moreover, this very same sample is made of highly compacted chromatin that can be studied at higher resolution, i.e., by means of scanning force microscopy. Fluorescence optical sectioning has been used to enter the spatial organisation. Three-dimensional images of single, twisted, and folded fibers are shown.
Diaspro
A
A
Department of Physics, University of Genoa, Italy.
Beltrame
F
F
Fato
M
M
Palmeri
A
A
Ramoino
P
P
D000818:Animals; D002843:Chromatin / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008297:Male; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal / Q000379:methods*; D049831:Octopodiformes; D013077:Sperm Head / Q000648:ultrastructure*
D002843:Chromatin