Evaluation of an on-line solid-phase extraction method for determination of almokalant, an antiarrhythmic drug, by liquid chromatography.
A fully automated liquid chromatographic method based on a Prospekt solid-phase extraction unit is described for determination of the antiarrhythmic drug almokalant in plasma. The assay comprises solid-phase extraction on a C2 phase and separation on a C18 column with fluorometric detection. In the original procedure 40 samples a day could be run unattended but by modifying the sequence in the solid-phase extraction process it was possible to increase this number to 70. The method gives an absolute recovery of 92% and a repeatability (C.V.) of 2.9% at 75 nmol/l of plasma. The limit of quantitation is 2 nmol/l of plasma (C.V. < 20%). As regards accuracy and precision the performance of the method is as good as the manual method based on liquid-liquid extraction. The Prospekt method is, above all, faster and requires far less manual effort.
Svennberg
H
H
Astra Hässle AB, Mölndal, Sweden.
Lagerström
P O
PO
D000889:Anti-Arrhythmia Agents / Q000097:blood*; D001331:Automation; D002853:Chromatography, Liquid / Q000379:methods*; D005069:Evaluation Studies as Topic; D015394:Molecular Structure; D011412:Propanolamines / Q000097:blood*
D000889:Anti-Arrhythmia Agents; D011412:Propanolamines; C074334:almokalant