Chronic treatment with antipsychotic drugs does not alter G protein alpha or beta subunit levels in rat brain.
Groups of rats received once daily subcutaneous treatments for 22 days with haloperidol (0.5 mg/kg), clozapine (20 mg/kg), SCH 23390 (0.2 mg/kg) or vehicle. Quantitative immunoblots for Gi alpha 1, Gi alpha 2, G(o alpha), Gs alpha (45 kD), G beta 35 and G beta 36 were performed on membranes from the following brain regions: striatum, nucleus accumbens, substantia nigra, ventral tegmental area (VTA), prefrontal cortex and hippocampus (CA1). No significant alterations were found in the levels of any of these G protein subunits, in any brain region in treated vs control rats.
Meller
E
E
New York University Medical Center, Department of Psychiatry, NY 10016, USA.
Bohmaker
K
K
D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D001552:Benzazepines / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003024:Clozapine / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006220:Haloperidol / Q000494:pharmacology*; D046911:Macromolecular Substances; D008297:Male; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values
D014150:Antipsychotic Agents; D001552:Benzazepines; D046911:Macromolecular Substances; D019204:GTP-Binding Proteins; D003024:Clozapine; D006220:Haloperidol