title: Assay for the (R)- and (S)-enantiomers of salsolinols in biological samples and foods with ion-pair high-performance liquid chromatography using beta-cyclodextrin as a chiral mobile phase additive.
abstract: A chromatographic procedure was devised for the quantitative determination of the enantiomers of salsolinol and N-methylsalsolinol, which are biologically important alkaloids. The enantiomers of salsolinol and N-methylsalsolinol were completely separated using beta-cyclodextrin in a reversed-phase ion-pair system. The HPLC method was sensitive enough to detect the isoquinolines at a concentration less than 0.1 pmol per injection. The presence of (R)- and (S)-salsolinol was confirmed in fermented foods and beverages, while N-methylsalsolinol was not detected. On the other hand, the (R)-enantiomers of both salsolinol and N-methylsalsolinol were found to predominate in the human brain.
authors.affiliation: Department of Applied Chemistry, Nagoya Institute of Technology, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Deng
authors.forename: W
authors.initials: W
authors.lastname: Maruyama
authors.forename: M
authors.initials: M
authors.lastname: Kawai
authors.forename: P
authors.initials: P
authors.lastname: Dostert
authors.forename: H
authors.initials: H
authors.lastname: Yamamura
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Naoi
mesh_terms: D001628:Beverages / Q000032:analysis; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry*; D005504:Food Analysis; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007546:Isoquinolines / Q000032:analysis*; D008968:Molecular Conformation; D015203:Reproducibility of Results; D012490:Salsoline Alkaloids / Q000032:analysis*; D044005:Tetrahydroisoquinolines; D014920:Wine / Q000032:analysis; D047392:beta-Cyclodextrins
chemical_list: D003505:Cyclodextrins; D007546:Isoquinolines; D012490:Salsoline Alkaloids; D044005:Tetrahydroisoquinolines; D047392:beta-Cyclodextrins; C036617:salsolinol; C032837:salsoline; C031215:betadex