Capillary gas chromatographic determination of permethrin insecticide by transesterification.
A sensitive method was developed to determine permethrin extracted from phosphate buffer and cattle plasma by potassium cyanide catalyzed transesterification of this insecticide with refluxing ethanol and detection of the resulting ethyl esters by capillary gas chromatography with an electron capture detector. With a reflux time of 2 h and with 3-phenoxybenzyl 2-chlorobenzoate as an internal standard, linear calibration curves from buffer (5-250 ng) and plasma (5-100 ng) were obtained. Precision and accuracy of the method were < or = 15%. The limit of detection was approximately 2.5 ng/ml (cis) and 1 ng/ml (trans) from buffer. In cattle sprayed along the back at 2 mg/kg, the concentration of cis- and trans-permethrin in plasma was below the detection limit (5 ng/ml).
Taylor
W G
WG
Research Centre, Agriculture and Agri-Food Canada, Canada.
Vedres
D D
DD
Hall
T W
TW
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D004951:Esterification; D000431:Ethanol; D007306:Insecticides / Q000097:blood*; D026023:Permethrin; D011190:Potassium Cyanide; D011722:Pyrethrins / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D007306:Insecticides; D011722:Pyrethrins; D000431:Ethanol; D026023:Permethrin; D011190:Potassium Cyanide