title: Hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a Pseudomonas species.
abstract: Hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera Pseudomonas, Moraxella, Arthrobacter and Aeromonas. A hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the Pseudomonas and Aeromonas spp. and in total soluble fractions of the Arthrobacter sp. A monomeric 19-kDa non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the Pseudomonas species and shown to be similar to hydroxylamine-cytochrome c oxidoreductase of Paracoccus denitrificans.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK.
authors.forename: J
authors.initials: J
authors.lastname: Wehrfritz
authors.forename: J P
authors.initials: JP
authors.lastname: Carter
authors.forename: S
authors.initials: S
authors.lastname: Spiro
authors.forename: D J
authors.initials: DJ
authors.lastname: Richardson
mesh_terms: D000333:Aeromonas / Q000201:enzymology; D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology; D003576:Electron Transport Complex IV / Q000302:isolation & purification* / Q000378:metabolism*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000378:metabolism*; D008969:Molecular Sequence Data; D009016:Moraxella / Q000201:enzymology; D009566:Nitrates / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010231:Paracoccus denitrificans / Q000201:enzymology; D011549:Pseudomonas / Q000201:enzymology* / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D006898:Hydroxylamines; D009566:Nitrates; D019811:Hydroxylamine; D010088:Oxidoreductases; C045582:ammonia monooxygenase; D003576:Electron Transport Complex IV