title: [Bacterial contamination of eyedrops in clinical use].
abstract: OBJECTIVE
  The aim of this study is to assess the importance of bacterial contamination of multidose eyedrops in a routine clinical setting.
  
  
  METHODS
  406 eyedrop vials were cultured about one week after clinical use: 204 collected from an Ophthalmic Department, and 202 from a Nursing Home. The microbiological analysis was performed on the tip and the residual eyedrop, counting the number of bacterial colonies.
  
  
  RESULTS
  66 (16.3%) from the 406 analyzed vials were contaminated, and 5.4% out of these were severely affected. There was no significant difference between the "Ophthalmic Department" and the "Nursing Home". Commensal germs were the most frequently encountered in both groups. 4 gram negative organisms were isolated from the "Nursing Home" group.
  
  
  CONCLUSIONS
  These results are in agreement with the literature. Comparison between our two groups is difficult because the eyedrops and uses were different. However, we notice the presence of gram negative organisms in the "Nursing Home". These severe contaminations due to opportunistic pathogen organisms are rare (0.75%), probably underestimated, and represent a real infectious risk during instillation. The study of the contamination site shows that the eyedrop is more often contaminated than the tip. This can be in relation to germ desicsation and to an aspiration phenomenon of contaminated fluid at the tip level. At last, the role of preservatives is not sufficient to ensure the sterility of multidose eyedrops during their use, and this justifies safer (single dose or filtration system) eyedrop vials.
authors.affiliation: Service d'Ophtalmologie, CHU G. Montpied, Clermont-Ferrand.
authors.forename: C
authors.initials: C
authors.lastname: Raynaud
authors.forename: H
authors.initials: H
authors.lastname: Laveran
authors.forename: D
authors.initials: D
authors.lastname: Rigal
authors.forename: P
authors.initials: P
authors.lastname: Bonicel
mesh_terms: D000328:Adult; D000368:Aged; D005128:Eye Diseases / Q000175:diagnosis / Q000188:drug therapy; D015818:Eye Infections, Bacterial / Q000209:etiology* / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000302:isolation & purification; D006707:Homes for the Aged; D006748:Hospital Departments; D006801:Humans; D009735:Nursing Homes; D009883:Ophthalmic Solutions / Q000009:adverse effects*; D009885:Ophthalmology; D012307:Risk Factors
chemical_list: D009883:Ophthalmic Solutions
vernacular_title: Etude de la contamination bactérienne de collyres en usage clinique.