Opioid-mediated responses of dietary protein on reticular motility and plasma insulin.
Four ruminally and abomasally cannulated steers (603 +/- 22.7 kg of body weight) were used to determine whether ruminally undegradable protein (RUP) would exert opioid-mediated effects on reticuloruminal motility or circulating concentrations of insulin. Steers were fed isonitrogenous diets (16% crude protein) containing either 30 or 40% RUP. The low RUP diet was supplemented with urea and soybean meal, and the high RUP diet was supplemented with blood meal, fish meal, corn gluten meal, and meat and bone meal. Diets contained 57% wheat silage and were fed twice daily at 0800 and 1600 h. Experimental periods were 10 d in length. Blood samples were taken from jugular catheters, and reticular motility was measured at hourly intervals on d 10 over a 16-h period. Either naltrexone (0.5 mg/kg of body weight) or saline was infused into the abomasum at the second feeding (9 h). Naltrexone reduced the frequency of reticular contractions by 16.5% for steers fed the low RUP diet. Naltrexone decreased the duration of reticular contractions by 9.3% for steers fed the low RUP diet and increased duration by 8.7% for steers fed the high RUP diet. Naltrexone decreased the opening time of the reticuloomasal orifice, expressed as a percentage of predose measurements, by 16.3% for steers fed the high RUP diet. Insulin was 21.3% higher with the high RUP diet. The postprandial rise in insulin decreased 36.7% with naltrexone. Dietary protein can exert effects mediated by opioids in ruminants.
Froetschel
M A
MA
Department of Animal and Dairy Science, University of Georgia, Athens 30602, USA.
Courchaine
J K
JK
Nichols
S W
SW
Amos
H E
HE
Murry
A C
AC
D000821:Animal Feed; D000818:Animals; D002417:Cattle / Q000502:physiology*; D004044:Dietary Proteins / Q000494:pharmacology*; D005769:Gastrointestinal Motility / Q000502:physiology*; D007328:Insulin / Q000097:blood*; D008297:Male; D009271:Naltrexone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D018847:Opioid Peptides / Q000502:physiology*; D012159:Reticulum / Q000502:physiology*; D012417:Rumen / Q000378:metabolism
D004044:Dietary Proteins; D007328:Insulin; D009292:Narcotic Antagonists; D018847:Opioid Peptides; D009271:Naltrexone