Transport of phosphate into vacuoles of Saccharomyces cerevisiae.
Isolated vacuoles of the yeast Saccharomyces cerevisiae accumulated orthophosphate at pH 6-7. This accumulation was inhibited by MgATP, and was insensitive to protonophores. Triton X-100 blocked this process. The accumulation increased linear by any phosphate concentrations employed (from 0.2 to 10 mM). It is proposed that phosphate is transported into yeast vacuoles via a channel transport system independent of the electrochemical proton gradient on the vacuolar membrane.
Kulakovskaya
T V
TV
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia.
Kulaev
I S
IS
D000255:Adenosine Triphosphate / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
D010710:Phosphates; D000255:Adenosine Triphosphate