title: Enzymatic syntheses of GlcNAc beta 1-2Man and Gal beta 1-4GlcNAc beta 1-2Man as components of complex type sugar chains.
abstract: GlcNAc beta 1-2Man and GlcNAc beta 1-6Man were synthesized using the reverse hydrolysis activity of beta-N-acetylglucosaminidase from both jack beans and Bacillus circulans. In turn, Gal beta 1-4GlcNAc beta 1-2Man and Gal beta 1-4GlcNAc beta 1-6Man were synthesized regioselectively using the transglycosylation activity of beta-galactosidase from Diplococcus pneumoniae and B. circulans, respectively. These di- and trisaccharides are important components of complex type sugar chains and will be used as intermediates in our synthetic studies.
authors.affiliation: Meiji Institute of Health Science, Naruda Odawara, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fujimoto
authors.forename: M
authors.initials: M
authors.lastname: Isomura
authors.forename: T
authors.initials: T
authors.lastname: Miyazaki
authors.forename: I
authors.initials: I
authors.lastname: Matsuo
authors.forename: R
authors.initials: R
authors.lastname: Walton
authors.forename: T
authors.initials: T
authors.lastname: Sakakibara
authors.forename: K
authors.initials: K
authors.lastname: Ajisaka
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000138:chemical synthesis* / Q000378:metabolism*; D006031:Glycosylation; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D013296:Streptococcus pneumoniae / Q000201:enzymology; D014312:Trisaccharides / Q000138:chemical synthesis* / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004187:Disaccharides; D014312:Trisaccharides; C106299:N-acetylglucosaminyl-1-2-mannopyranose; C106298:galactopyranosyl-1-4-N--acetylglucosaminyl-1-2-mannopyranose; D001616:beta-Galactosidase; D000118:Acetylglucosaminidase