title: Cytoplasmic DNA in Entamoeba histolytica: its biological significance.
abstract: We report a study on the DNA organization in Entamoeba histolytica using a ribosomal DNA probe. The rDNA genes were found forming mers which were separated in a typical ladder pattern by pulse field electrophoresis. DNA rosette structures were visualized through electron microscopy in DNA eluted from bands recognized by the ribosomal probe. The in situ hybridization experiments using a DNA probe suggested that the rDNA genes are portioned between the nucleus and a cytoplasmic structure. These findings provide new data on DNA organization in E. histolytica and open the question concerning the presence of a novel organelle in this eukaryotic parasite.
authors.affiliation: Departamento de Patología Experimental, CINVESTAV IPN, México, D.F.
authors.forename: M
authors.initials: M
authors.lastname: Báez-Camargo
authors.forename: T
authors.initials: T
authors.lastname: Sánchez
authors.forename: F
authors.initials: F
authors.lastname: Solis
authors.forename: M A
authors.initials: MA
authors.lastname: Rodríguez
authors.forename: G
authors.initials: G
authors.lastname: Contreras
authors.forename: P
authors.initials: P
authors.lastname: Chávez
authors.forename: A M
authors.initials: AM
authors.lastname: Riverón
authors.forename: E
authors.initials: E
authors.lastname: Orozco
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry*; D016054:DNA, Protozoan / Q000235:genetics* / Q000648:ultrastructure; D004275:DNA, Ribosomal / Q000235:genetics* / Q000648:ultrastructure; D016521:Electrophoresis, Gel, Pulsed-Field; D004748:Entamoeba histolytica / Q000235:genetics* / Q000648:ultrastructure; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D015388:Organelles
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal