Evaluation of rapid molecular methods for detection of clarithromycin resistance in Helicobacter pylori.
Resistance of Helicobacter pylori to clarithromycin is due to point mutations at position A2143 or A2144 of the rrnH 23S rRNA gene, each mutation creating an additional restriction site for BsaI or MboII. A procedure combining PCR and RFLP analysis was evaluated for detection of these mutations using primers specific for the 23S rRNA gene, and BsaI and MboII enzymes. All clarithromycin-resistant isolates (8/8), as defined by the MIC, were found to be resistant by PCR-RFLP. No clarithromycin-sensitive isolates (14/14) gave a positive reaction.
Szczebara
F
F
Laboratoire de Bactériologie-Hygiène, Faculté de médecine, Lille, France.
Dhaenens
L
L
Vincent
P
P
Husson
M O
MO
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial / Q000235:genetics; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S; D017291:Clarithromycin