Deletion of nef slows but does not prevent CD4-positive T-cell depletion in human immunodeficiency virus type 1-infected human-PBL-SCID mice.
The pathogenicity of four human immunodeficiency virus type 1 (HIV-1) isolates with nef deleted for SCID mice repopulated with human peripheral blood leukocytes (hu-PBL-SCID mice) was studied. Deletion of nef led to a substantial reduction in CD4-positive T-cell depletion and delayed kinetics of plasma viremia in infected hu-PBL-SCID mice. Deletion of the nef gene impacts both the efficiency of primary infection and the cytopathicity of virus for infected CD4-positive T cells in this animal model of HIV-1 infection.
Gulizia
R J
RJ
Department of Immunology-IMM7, The Scripps Research Institute, La Jolla, California 92037, USA.
Collman
R G
RG
Levy
J A
JA
Trono
D
D
Mosier
D E
DE
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D017353:Gene Deletion; D015754:Genes, nef / Q000502:physiology*; D015497:HIV-1; D006801:Humans; D007962:Leukocytes / Q000821:virology*; D051379:Mice; D016513:Mice, SCID; D012367:RNA, Viral / Q000032:analysis
D012367:RNA, Viral