Simultaneous detection of near-field topographic and fluorescence images of human chromosomes via scanning near-field optical/atomic-force microscopy (SNOAM).
Scanning near-field optical/atomic-force microscopy (SNOAM) provided us with simultaneous topographical and optical images of human chromosomes using a sharp and bent optical fiber as a near-field optical probe. Native chromosomes were spread out onto a coverslip using the surface-spreading whole-mount method. The SNOAM system does not need pretreatment of samples such as metal coating or chemical immobilization. Near-field topographic and fluorescence images provided useful information on native chromosome structure.
Iwabuchi
S
S
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Tatsunokuchi, Ishikawa 923-12, Japan.
Muramatsu
H
H
Chiba
N
N
Kinjo
Y
Y
Murakami
Y
Y
Sakaguchi
T
T
Yokoyama
K
K
Tamiya
E
E
D001402:B-Lymphocytes; D052160:Benzothiazoles; D002460:Cell Line; D002877:Chromosomes, Human / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis; D003959:Diamines; D005456:Fluorescent Dyes; D006801:Humans; D008677:Metaphase; D018625:Microscopy, Atomic Force / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D009930:Organic Chemicals; D011804:Quinolines
D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA