Bluetongue virus in laboratory-reared Culicoides variipennis sonorensis: applications of dot-blot, ELISA, and immunoelectron microscopy.
An avidin-biotin complex (ABC) dot-blot, an antigen capture enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy (IEM) were used to detect bluetongue (BLU) virus or viral antigen or both in adult Culicoides variipennis sonorensis Wirth & Jones. The dot-blot and ELISA procedures detected viral antigen in 10-22% (depending on serotype) of the biting midges infected with BLU-2, BLU-10, BLU-13, and BLU-17 and approximately 68% of the midges infected with BLU-11. IEM analyses revealed BLU virus in salivary glands, fat body, and thoracic muscle tissue from infected insects. There appeared to be selective growth of the virus in salivary gland tissue.
Nunamaker
R A
RA
Arthropod-Borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, WY 82071, USA.
Mecham
J O
JO
Wigington
J G
JG
Ellis
J A
JA
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001820:Bluetongue virus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D002460:Cell Line; D002519:Ceratopogonidae / Q000821:virology*; D003365:Costs and Cost Analysis; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016253:Microscopy, Immunoelectron
D000956:Antigens, Viral