Structures of sulfated oligosaccharides isolated from the respiratory mucins of a non-secretor (O, Le(a + b -)) patient suffering from chronic bronchitis.
Mucin glycopeptides were prepared from the respiratory mucus of a non-secretor, chronic bronchitic patient with blood group O, Le(a + b-). Oligosaccharides were released by alkaline borohydride treatment and purified by anion-exchange chromatography, size-exclusion chromatography and high performance anion-exchange chromatography. Structural studies employed 400-MHz 1H-NMR spectroscopy and matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Nine monosulfated oligosaccharides ranging in size from tetra- to hexasaccharide, were fully characterized in this study. The sulfate group occurs either on the C-3 of a terminal galactose residue or on the C-6 of a N-acetylglucosamine residue. In keeping with the non-secretor status of the patient, no structure with an (alpha 1-2)-linked fucose residue was found. Five of the structures had fucose present in (alpha 1-3)-linkage in the X determinant, while only one oligosaccharide (compound 7b) was seen with fucose (alpha 1-4)-linked in the Le(a) determinant. Eight structures isolated from the mucins of the non-secretor patient had not been found previously in the respiratory mucins; they are listed below.
Lo-Guidice
J M
JM
INSERM U377, Lille, France.
Herz
H
H
Lamblin
G
G
Plancke
Y
Y
Roussel
P
P
Lhermitte
M
M
D000017:ABO Blood-Group System / Q000276:immunology / Q000378:metabolism; D001991:Bronchitis / Q000378:metabolism*; D002240:Carbohydrate Sequence; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D009844:Oligosaccharides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D012137:Respiratory System / Q000737:chemistry* / Q000378:metabolism; D013183:Sputum / Q000737:chemistry; D013431:Sulfates
D000017:ABO Blood-Group System; D007983:Lewis Blood Group Antigens; D009077:Mucins; D009844:Oligosaccharides; D013431:Sulfates