Immunofluorescent study after transient transfection of myoblast cells with the plasmid PSV40-beta-globin.
The level of transfection of myoblast C-2/C-12 cells with the plasmid PSV40-beta-globin, using the immunofluorescence assay, was investigated. Myoblasts, which are non-terminally differentiated cells, were chosen since it is known that terminally differentiated myotubes are very poorly transfected. The results showed that myoblast cells of the C-2/C-12 cell line were readily transfected within 24 h with the PSV40-beta-globin gene. The immunofluorescence assay showed the nucleoplasmic localization of the SV40 large T antigen, and indicated the possibility that some fraction of it might be localized in the cytoplasm.
Pironcheva
G L
GL
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Russev
G
G
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis* / Q000235:genetics; D002130:Calcium Phosphates; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005822:Genetic Vectors; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008856:Microscopy, Fluorescence; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D002130:Calcium Phosphates; D011993:Recombinant Fusion Proteins; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D005914:Globins; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous