title: A sudden death syndrome induced in poults and chicks fed diets containing Fusarium fujikuroi with known concentrations of moniliformin.
abstract: A sudden death syndrome was induced in chicks and poults fed diets containing Fusarium fujikuroi, formulated to contain 0-330 mg/kg moniliformin (M) with or without the maximum recommended therapeutic concentration of monensin. Lesions of monensin toxicosis were not observed. Clinical signs were referable to cardiac dysfunction (sudden death, dyspnea, cyanosis, depression). Poults and chicks dying early in the study had no gross lesions or had lesions of right ventricular dilation. Treated poults and chicks dying late in the study or euthanatized at termination of the study had lesions of bilateral myocardial hypertrophy, usually concentric. Absolute heart weights and relative heart weights, expressed as a percentage of body weight, were significantly greater in treated birds than controls (P < 0.05), whereas body weights were significantly less (P < 0.05). Microscopically, lesions progressed from acute myocardial degeneration to necrosis, fibrosis, and hypertrophy. Ultrastructural findings were consistent with the gross and microscopic lesions. Serum pyruvate concentrations were a useful indicator of M-induced cardiotoxicosis. Concentrations of serum pyruvate increased with increased concentration of dietary M, but were not affected by addition of monensin to the diet. In chicks ingesting 40-300 mg/kg M, serum pyruvate concentrations were significantly greater (P > 0.05) than those in controls (controls, 0.28 +/- 0.08 mmol/liter; exposed 0.38 +/- 0.11-0.55 +/- 0.13 mmol/liter). Poults ingesting 80-330 mg/kg M had significantly greater serum pyruvate concentrations than controls (controls 0.33 +/- 0.09 mmol/liter; exposed 0.43 +/- 0.13-1.00 +/- 0.006 mmol/liter). The Vetronics System was used to evaluate electrocardiographic alterations in a limited number of chicks and poults surviving to the end of the feeding trial. Electrocardiographic alterations in poults and chicks fed diets containing > or = 40 mg/kg and > or = 160 mg/kg M, respectively, were consistent with ventricular hypertrophy, myocardial injury, and hypoxia. Electrocardiographic alterations were more striking in poults than in chicks. Altered myocardial metabolism due to M toxicosis, in conjunction with the unusual susceptibility of domestic poultry to altered cardiac metabolism, is believed to be the cause of the organ-specific lesions in these birds. These findings suggest that cardiac injury with subsequent alterations in cardiac electrical conductance may be a cause of the sudden deaths observed in poultry chronically intoxicated with dietary M.
authors.affiliation: Department of Veterinary Pathobiology, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: R Y
authors.initials: RY
authors.lastname: Reams
authors.forename: H L
authors.initials: HL
authors.lastname: Thacker
authors.forename: D D
authors.initials: DD
authors.lastname: Harrington
authors.forename: M N
authors.initials: MN
authors.lastname: Novilla
authors.forename: G E
authors.initials: GE
authors.lastname: Rottinghaus
authors.forename: G A
authors.initials: GA
authors.lastname: Bennett
authors.forename: J
authors.initials: J
authors.lastname: Horn
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use; D001835:Body Weight; D002645:Chickens; D003503:Cyclobutanes / Q000008:administration & dosage / Q000633:toxicity*; D016757:Death, Sudden, Cardiac / Q000209:etiology / Q000517:prevention & control / Q000662:veterinary*; D004032:Diet; D004562:Electrocardiography; D005670:Fusarium; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D008985:Monensin / Q000627:therapeutic use*; D009183:Mycotoxins / Q000008:administration & dosage / Q000633:toxicity*; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure; D009929:Organ Size; D011773:Pyruvates / Q000097:blood
chemical_list: D000935:Antifungal Agents; D003503:Cyclobutanes; D009183:Mycotoxins; D011773:Pyruvates; C008539:moniliformin; D008985:Monensin