title: Evaluation of the trophic effect of human placental polydeoxyribonucleotide on human knee skin fibroblasts in primary culture.
abstract: A simple assay capable of evaluating the trophic effect of growth factors or active principles on human skin diploid fibroblasts in primary culture has been developed. The results indicate that at physiological concentrations (20-100 micrograms/ml) a human placental polydeoxyribonucleotide (PDRN) preparation enhances the growth of human skin diploid fibroblasts of the knee in primary culture. This effect is consistently reproducible in the case of patients over 60 years of age, and may explain previously reported data on the successful clinical applications of human placental preparations, suggesting a selective benefit of PDRN in wound healing when compared to other treatments.
authors.affiliation: Institute of Microbiology, University of Genoa, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Muratore
authors.forename: A
authors.initials: A
authors.lastname: Pesce Schito
authors.forename: G
authors.initials: G
authors.lastname: Cattarini
authors.forename: E L
authors.initials: EL
authors.lastname: Tonoli
authors.forename: S
authors.initials: S
authors.lastname: Gianoglio
authors.forename: S
authors.initials: S
authors.lastname: Schiappacasse
authors.forename: L
authors.initials: L
authors.lastname: Felli
authors.forename: F
authors.initials: F
authors.lastname: Picchetta
authors.forename: G C
authors.initials: GC
authors.lastname: Schito
mesh_terms: D002460:Cell Line; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007717:Knee; D010920:Placenta / Q000378:metabolism*; D011089:Polydeoxyribonucleotides / Q000494:pharmacology*; D011247:Pregnancy; D014945:Wound Healing / Q000187:drug effects
chemical_list: D011089:Polydeoxyribonucleotides