title: The repair of UV-induced cyclobutane pyrimidine dimers in the individual genes Gart, Notch and white from isolated brain tissue of Drosophila melanogaster.
abstract: We have measured the induction and removal of UV-induced cyclobutane pyrimidine dimers from defined, DNA sequences in brains isolated from wild-type Drosophila melanogaster third instar larvae. Brains were exposed to a single dose of 500 J/m2 UVB and kept in the dark for up to 48 h. Within 48 h after irradiation, 50% of the dimers are removed from the actively transcribed genes Gart and Notch. Moreover, these kinetics are similar to the time course of dimer removal measured in the transcriptionally inactive white gene. It is further demonstrated that the genome overall is repaired at a similar rate. The results are discussed with respect to the in vivo irradiation of brains and to the data found for gene-specific repair in other eukaryotes.
authors.affiliation: MGC-Department of Radiation Genetics and Chemical Mutagenesis, University of Leiden, The Netherlands.
authors.forename: P J
authors.initials: PJ
authors.lastname: van der Helm
authors.forename: E C
authors.initials: EC
authors.lastname: Klink
authors.forename: P H
authors.initials: PH
authors.lastname: Lohman
authors.forename: J C
authors.initials: JC
authors.lastname: Eeken
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism; D003503:Cyclobutanes / Q000494:pharmacology*; D004247:DNA / Q000302:isolation & purification; D015342:DNA Probes / Q000235:genetics; D004260:DNA Repair; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D004720:Endonucleases / Q000378:metabolism; D005136:Eye Proteins; D017344:Genes, Insect / Q000187:drug effects* / Q000528:radiation effects*; D019476:Insect Proteins / Q000235:genetics*; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics*; D011740:Pyrimidine Dimers / Q000378:metabolism; D051880:Receptors, Notch; D014158:Transcription, Genetic; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D018528:ATP-Binding Cassette Transporters; D003503:Cyclobutanes; D015342:DNA Probes; D029721:Drosophila Proteins; D005136:Eye Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D011740:Pyrimidine Dimers; D051880:Receptors, Notch; C059254:w protein, Drosophila; D004247:DNA; D004720:Endonucleases