Single intracranial injection of apotransferrin in young rats increases the expression of specific myelin protein mRNA.
Transferrin (Tf) is a possible regulator of oligodendrocyte development in vitro (Espinosa de los Monteros et al., 1989). At least two different mechanisms may account for the effects of Tf on myelin synthesis. It may act as a trophic factor and enhance the formation of new myelin sheaths. Tf may also induce the synthesis of myelin proteins in the central nervous system. We recently demonstrated that a single intracranial injection of apotransferrin (aTf) in young rats induces an increased myelination (Escobar Cabrera et al., 1994). In the present study, we investigated the in vivo effect of aTf on the expression of mRNAs of specific myelin genes. Three-day-old rats were injected intracranially with aTf and killed at different ages after injection. Total brain RNA was isolated, and the expression of different mRNAs was analyzed by Northern blot. The amount of mRNAs of myelin basic protein and of 2'-3' cyclic nucleotide 3'-phosphohydrolase were markedly increased in the experimental animals, whereas myelin proteolipid protein mRNA did not show differences relative to controls. These results indicate that in the animals treated with aTf, there is a differential effect on the expression of certain specific myelin protein genes. They also suggest that aTf might exert its action at the posttranscriptional level and/or by direct transcriptional regulation of the genes.
Escobar Cabrera
O E
OE
Departmento de Química Biológica, Facultad de Farmacia y Bioquímica,Universidad de Buenos Aires-Instituto de Química y Fisicoquímica Biológica, Argentina.
Zakin
M M
MM
Soto
E F
EF
Pasquini
J M
JM
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000096:biosynthesis; D000818:Animals; D000831:Animals, Newborn; D001059:Apoproteins / Q000008:administration & dosage / Q000494:pharmacology*; D015152:Blotting, Northern; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D015342:DNA Probes; D008845:Microinjections; D004676:Myelin Basic Protein / Q000096:biosynthesis*; D018991:Myelin Proteolipid Protein / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014168:Transferrin / Q000008:administration & dosage / Q000494:pharmacology*
D001059:Apoproteins; D015342:DNA Probes; D004676:Myelin Basic Protein; D018991:Myelin Proteolipid Protein; D012333:RNA, Messenger; D014168:Transferrin; C028284:apotransferrin; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases