title: Subspecificities of anti-M and anti-N antibodies tested with chemically-modified antigens.
abstract: The division of anti-M and anti-N antibodies into subspecificities based on dependence of the epitope on amino acid residue 1 or 5 of GPA, amino groups and sialic acid residues is presented. The Workshop antibodies were classified into the subspecificities by testing their reactivity with GPA-M or GPA-N untreated and modified by Edman degradation (release of the first amino acid residue), periodate oxidation, N-acetylation and desialylation. Most antibodies, according to former results, belonged to the group recognizing the blood group M- or N-related epitope dependent on the first amino acid residue, amino group and sialic acid residues, but other subspecificities were also found.
authors.affiliation: Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Duk
authors.forename: E
authors.initials: E
authors.lastname: Lisowska
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D006021:Glycophorins / Q000737:chemistry / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D007519:Isoantigens / Q000737:chemistry*; D008951:MNSs Blood-Group System / Q000276:immunology*; D017186:Titrimetry
chemical_list: D000911:Antibodies, Monoclonal; D006021:Glycophorins; D007519:Isoantigens; D008951:MNSs Blood-Group System