Supercritical fluid extraction and reversed-phase liquid chromatography methods for vitamin A and beta-carotene heterogeneous distribution of vitamin A in the liver.
We developed supercritical fluid procedures for extracting vitamin A and beta-carotene from vitamin supplements and calf liver tissue. The SF extracts could be injected onto an HPLC column without further pretreatment. Samples were analysed by RP-HPLC using diode array detection or by spectrophotometry. Recoveries were very good. SF extracts from a vitamin preparation of uniform composition had an R.S.D. of 4%. Extracts from calf liver supplements were predictably more heterogeneous. The SF extraction method is less labor intensive then traditional liquid-liquid procedures for extracting vitamin A and carotenoids from tissues.
Burri
B J
BJ
Western Human Nutrition Research Center, USDA/ARS/PWA, San Francisco, CA 94129, USA.
Neidlinger
T R
TR
Lo
A O
AO
Kwan
C
C
Wong
M R
MR
D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008099:Liver / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014801:Vitamin A / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D019207:beta Carotene / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D000975:Antioxidants; D019207:beta Carotene; D014801:Vitamin A