Determination of genotoxicity of the metabolites of the pesticides Guthion, Sencor, Lorox, Reglone, Daconil and Admire by 32P-postlabeling.
Commercial formulations of the pesticides: Guthion (azinphos methyl), Sencor (metribuzin), Lorox (linuron), Reglone (diquat), Daconil (chlorothalonil) and Admire (imidacloprid) were studied for their genotoxicity by 32P-postlabeling. Metabolites of the pesticides were obtained enzymatically using arochlor induced rat liver S9 fraction, in an NADPH generating system. The resulting metabolites were reacted with calf thymus DNA and the DNA was analyzed for presence of adducts by either the nuclease P1 or butanol enrichment. Nuclease P1 enrichment resulted in adducts for all the pesticides. Compared to the level of adducts in control DNA, the levels in pesticide-treated DNA were higher for all the pesticides, except Daconil. The increase in adduct numbers for pesticide-treated DNAs ranged from 4.9-12.4 times the control-DNA indicating pesticide genotoxicity in this in vitro system. Enrichment using butanol extraction gave three adducts unique to Sencor-DNA. These adducts were different from those obtained with nuclease P1 enrichment of the same. B(alpha)P was the positive control for the in vitro metabolism, and two adduct enrichment procedures: nuclease P1 digestion and butanol extraction.
Shah
R G
RG
Unit of Health and Environment, CHUL Research Center, Ste-Foy, Québec, Canada.
Lagueux
J
J
Kapur
S
S
Levallois
P
P
Ayotte
P
P
Tremblay
M
M
Zee
J
J
Poirier
G G
GG
D000255:Adenosine Triphosphate; D000818:Animals; D000440:Butanols; D002417:Cattle; D002855:Chromatography, Thin Layer; D004247:DNA / Q000187:drug effects*; D018736:DNA Adducts / Q000032:analysis*; D008862:Microsomes, Liver / Q000378:metabolism; D010575:Pesticides / Q000378:metabolism / Q000633:toxicity*; D010761:Phosphorus Radioisotopes; D051381:Rats; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013950:Thymus Gland
D000440:Butanols; D018736:DNA Adducts; D010575:Pesticides; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases