Removal of chromosomal DNA by Mg2+ in the lysis buffer: an improved lysis protocol for preparing Escherichia coli whole-cell lysates for sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Chen
M
M
Biochemisches Institut, Universität Zürich, Switzerland.
Christen
P
P
D002021:Buffers; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D008274:Magnesium; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D002021:Buffers; D004269:DNA, Bacterial; D012967:Sodium Dodecyl Sulfate; D008274:Magnesium