title: Generation of superoxide anion and induction of superoxide dismutase and peroxidase in bean leaves infected with pathogenic fungi.
abstract: Generation of superoxide anion (O2-.) and peroxidase activity were significantly increased in bean leaves infected with incompatible and compatible pathogens: Botrytis fabae and Botrytis cinerea, respectively, but the induction was greater on direct inoculation with B. fabae, than with B. cinerea. A slightly higher O2-. level was also detected in the parts of leaves surrounding the inoculation side. Overproduction of O2-. was observed earlier than the increase in peroxidase activity. Pretreatment of the leaves with methyl jasmonate enhanced both O2-. production and peroxidase activity following inoculation with B. cinerea. Induction of superoxide dismutase activity after the infection was less pronounced than changes in O2-. level. The differences in the rate of NADH oxidation in the extracts from control and inoculated leaves, correlated with the differences in the rate of O2-. production. The results indicate that O2-. level is one of the essential factors responsible for the difference in the interactions between bean plant and compatible and incompatible pathogens.
authors.affiliation: Department of Plant Physiology and Biochemistry, University of Lódź, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Urbanek
authors.forename: E
authors.initials: E
authors.lastname: Gajewska
authors.forename: R
authors.initials: R
authors.lastname: Karwowska
authors.forename: M
authors.initials: M
authors.lastname: Wielanek
mesh_terms: D007887:Fabaceae / Q000201:enzymology* / Q000382:microbiology; D003904:Mitosporic Fungi / Q000302:isolation & purification* / Q000472:pathogenicity; D009195:Peroxidase / Q000096:biosynthesis*; D018515:Plant Leaves / Q000201:enzymology* / Q000382:microbiology; D010946:Plants, Medicinal; D013482:Superoxide Dismutase / Q000096:biosynthesis*; D013481:Superoxides / Q000378:metabolism*
chemical_list: D013481:Superoxides; D009195:Peroxidase; D013482:Superoxide Dismutase