Immunoblot characterization of IgG, IgM and IgE antibodies elicited during the course of fatal and non-fatal infections of Plasmodium berghei in DBA/2 and Balb/c mice.
When infected with the CRS line of Plasmodium berghei ANKA, DBA/2 mice died of a fulminating infection around day 20 post-infection whereas Balb/c mice had crises around day 15 then low or subpatent parasitaemias until approximately day 73, when sterile immunity is believed to have supervened. Immunoblots for parasite-specific immunoglobulins G, M and E were made from the sera taken during the course of infection in each mouse strain. Although both strains elicited antibodies to a 128-kDa antigen by day 8, this was solely of the IgG class in the DBA/2 but of both IgG and IgM in the Balb/c. Crisis and subpatency were associated with the appearance of IgG and IgM antibodies to 74- and 80-kDa antigens. The most vigorous immune response was observed in sera from the Balb/c mice on day 73, with antibodies to many antigens of 19-180 kDa; IgE antibody (to antigens of 38 and 45 kDa) was then evident for the first time.
Desowitz
R S
RS
Prince Leopold Institute of Tropical Medicine, Antwerp, Belgium.
van Overmeir
C
C
Wéry
M
M
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000302:isolation & purification; D005260:Female; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008137:Longitudinal Studies; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D018512:Parasitemia / Q000276:immunology; D010962:Plasmodium berghei / Q000276:immunology*; D013997:Time Factors
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E