[Immunoenzyme method of the determination of human chorionic gonadotropin using polyclonal antibodies].
The authors developed a method for obtaining highly specific polyclonal antibodies reacting with different sites of human chorionic gonadotropin (HCG) and an immunometrical method for measuring HCG in human biological fluids, based on the use of these antibodies. The sensitivity of the method is 10-15 IU/liter HCG, specificity 100%, no cross reactions with LH or FSH were observed. The method was tried in testing urine samples for HCG in women at 1-2 to 34 weeks of gestations and in one cancer patient with the diagnosis of vesical mole.
Iurkina
E A
EA
Ofitserov
V I
VI
Samukov
V V
VV
Dedkova
L M
LM
Mizenko
G A
GA
Reshetnikov
S S
SS
Frolova
S B
SB
Kalashnikov
V V
VV
Sabirov
A N
AN
Ris
V I
VI
D000818:Animals; D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin / Q000652:urine*; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D011247:Pregnancy; D011817:Rabbits; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D007106:Immune Sera
Immunofermentnyĭ metod opredeleniia khorionicheskogo gonadotropina cheloveka s ispol'zovaniem poliklonal'nykh antitel.