Development of a high-performance liquid chromatographic-atmospheric pressure chemical ionization-tandem mass spectrometric assay for beta-tigogenin cellobioside in human serum.
A specific high-performance liquid chromatographic-atmospheric pressure chemical ionization tandem mass spectrometric assay for the quantitative determination of beta-tigogenin cellobioside in human serum is described. Serum cleanup and acetylation of the analyte were required to achieve the desired lower limit of quantification, 10 ng/ml. The precision of the assay was better than 13% over a serum concentration range of 10-500 ng/ml.
Avery
M J
MJ
Central Research Division, Pfizer Inc., Groton, CT 06340, USA.
Fouda
H G
HG
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D012503:Saponins / Q000097:blood*
D012503:Saponins; C080606:tiqueside