[The cloning and analysis of a single gene in Drosophila homologic with human oncogene TTG].
The human TTG/RBTN family is an oncogene family. There are three members in this family: TTG-1/RBTN-1, TTG-2/RBTN-2 and TTG-3/RBTN-3. Two of them, TTG-1/RBTN-1 and TTG-2/RBTN-2 have been isolated at the sites of chromosomal translocations in T-cell leukaemia. This gene family encodes cysteine-rich proteins with two tandem copies of a LIM motif. The function of the LIM motif is unknown. We found that the TTG-2 gene is highly conserved among mammals; Drosophila and yeast. As a first step to obtain a model system for studying the function of the LIM motifs, we isolated the Drosophila homologue dttg. In contrast to human, Drosophila appeared to have only one ttg/rbtn gene. A 2087bp cDNA clone was isolated, encoding a protein of 266 amino acids. A second transcript with an alternative 5' end was identified in RNA from embryos. The Drosophila ttg protein consisted of two tandem copies of the conserved LIM domain characteristic of the human TTG/RBTN family. The amino acid sequence similarity with human TTG-1 and TTG-2 is 79% and 62%, respectively. The dttg, like TTG-1, have an intron in the second LIM encoding region, which is not present in TTG-2.
Zhu
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Medical College of Nankai University, Tianjing.
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Royer-pokora
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D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D006801:Humans; D060588:LIM Domain Proteins; D008667:Metalloproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D011519:Proto-Oncogenes; D014157:Transcription Factors
D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; C065072:LMO1 protein, human; C085117:LMO2 protein, human; D008667:Metalloproteins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors