Multilineage gene expression precedes commitment in the hemopoietic system.
We have tested the hypothesis that multipotential hemopoietic stem and progenitor cells prime several different lineage-affiliated programs of gene activity prior to unilineage commitment and differentiation. Using single cell RT-PCR we show that erythroid (beta-globin) and myeloid (myeloperoxidase) gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythroid or granulocytic lineages. Furthermore, the multipotential state is characterized by the coexpression of several lineage-affiliated cytokine receptors. These data support a model of hemopoietic lineage specification in which unilineage commitment is prefaced by a "promiscuous" phase of multilineage locus activation.
Hu
M
M
The Leukaemia Research Fund Centre at the Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Krause
D
D
Greaves
M
M
Sharkis
S
S
Dexter
M
M
Heyworth
C
C
Enver
T
T
D000818:Animals; D002460:Cell Line; D004920:Erythropoiesis / Q000235:genetics; D015870:Gene Expression; D005914:Globins / Q000235:genetics; D006410:Hematopoiesis / Q000235:genetics* / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D009195:Peroxidase / Q000235:genetics; D018121:Receptors, Cytokine / Q000235:genetics; D017978:Receptors, Growth Factor / Q000235:genetics
D018121:Receptors, Cytokine; D017978:Receptors, Growth Factor; D005914:Globins; D009195:Peroxidase