A freeze-fracture study of the membrane morphology of phosphatidylethanolamine-deficient Escherichia coli cells.
Freeze-fracture electron microscopy was applied to study membrane morphology in a phosphatidylethanolamine-deficient E. coli strain. For growth, this strain requires millimolar concentrations of specific divalent cations like Mg2+ or Ca2+. These cations bring the bilayer to nonbilayer phase transition temperature of the lipids back to wild type levels by shifting the phase preference of cardiolipin in the membrane towards the inverted hexagonal (H(II)) phase. Under growth conditions, these cells show a bilayer based membrane with an intramembrane particle distribution as in wild type cells. Upon lowering the temperature, smooth areas are observed corresponding to gel state lipid bilayer domains. Ca2+ was used to manipulate the phase behavior of the membrane lipids in situ. Exposing the cells to Ca2+ up to 100 mM at 42 degrees C did not result in the appearance of nonbilayer structures, despite the fact that in total lipid extracts under these conditions the hexagonal H(II) phase was observed. However, the addition of a Ca2+ ionophore, which leads to exposure to Ca2+ of both faces of the plasma membrane, gives rise to formation of H(II) phase, stacked bilayer domains and blebbing upon addition of 50 mM CaCl2 at 42 degrees C. We conclude that the asymmetrical localization of divalent cations in the periplasm of this strain allows them to be functionally effective while membrane stability is maintained.
Rietveld
A G
AG
Department Biochemistry of Membranes, Utrecht University, The Netherlands. rietveld@embl-heidelberg.de
Verkleij
A J
AJ
de Kruijff
B
B
D018528:ATP-Binding Cassette Transporters; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium; D002152:Calorimetry, Differential Scanning; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure*; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000737:chemistry / Q000648:ultrastructure*; D029968:Escherichia coli Proteins; D005614:Freeze Fracturing; D007476:Ionophores / Q000494:pharmacology; D008051:Lipid Bilayers / Q000737:chemistry*; D008274:Magnesium; D058619:Maltose-Binding Proteins; D008854:Microscopy, Electron / Q000379:methods; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D010714:Phosphatidylethanolamines
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D007476:Ionophores; D008051:Lipid Bilayers; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D010714:Phosphatidylethanolamines; C461599:maltose transport system, E coli; D000001:Calcimycin; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium