title: Rapid anterograde axonal transport of the syntaxin-SNAP 25-VAMP complex.
abstract: During the process of docking and fusion of synaptic vesicles to the presynaptic membrane, several presynaptic proteins bind sequentially to a core complex associating two proteins of the presynaptic membrane, syntaxin and SNAP 25, and a protein of synaptic vesicles, VAMP/synaptobrevin. We have immunoprecipitated this core complex after CHAPS solubilization of pure cholinergic synaptosomes of Torpedo electric organ, using anti-syntaxin or anti-VAMP immunobeads. In parallel, we studied syntaxin and VAMP, which are transported by the rapid axonal flow to the nerve endings. We found that syntaxin and VAMP accumulating at the proximal end of an electric nerve ligature were already engaged in complexes, as in synaptosomes. In unligated nerves also, significant amounts of VAMP associate with syntaxin. The possibility that these complexes form after solubilization was eliminated because added VAMP was unable to associate with syntaxin in solubilized control nerves and because similar amounts of complex were obtained after sodium dodecyl sulfate or CHAPS solubilization. Hence, syntaxin is already associated with SNAP 25 and VAMP during axonal transport, before reaching nerve endings.
authors.affiliation: Laboratoire de Neurobiologie Cellulaire et Moleculaire, CNRS, Gif-sur-Yvette, France.
authors.forename: G
authors.initials: G
authors.lastname: Shiff
authors.forename: N
authors.initials: N
authors.lastname: Morel
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D004557:Electric Organ / Q000737:chemistry / Q000294:innervation / Q000502:physiology; D008026:Ligation; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D008863:Microspheres; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D011233:Precipitin Tests; D017729:Presynaptic Terminals / Q000737:chemistry / Q000378:metabolism; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D050825:Synaptosomal-Associated Protein 25; D013574:Synaptosomes / Q000737:chemistry; D014101:Torpedo
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D050825:Synaptosomal-Associated Protein 25