The S3a ribosomal protein gene is identical to the Fte-1 (v-fos transformation effector) gene and the TNF-alpha-induced TU-11 gene, and its transcript level is altered in transformed and tumor cells.
Previous work on mouse x rat hybrid cells (BS series) led to the assignment of a transformation suppressor locus (Sail) to the rat 5q22-q33 region. This gene is not yet identified. From a non-transformed BS hybrid cell line, we isolated a partial cDNA insert (13T), which detects a transcript more abundant in transformed cells than in their non-transformed homologs. Sequence comparisons led us to conclude that 13T is identical to the coding sequences of the ribosomal protein S3a gene (Rps3a), of Fte-1 (v-fos transformation effector gene) and of TU-11, a mouse gene induced by TNF-alpha. Rps3a, Fte-1 and TU-11 are thus one and the same gene. Similarity was also found between this gene and non-mammalian sequences reported to be involved in cell cycling. Like the Rps3a transcript level, the c-Fos transcript level is higher in transformed cells. Rps3a and Fos could thus be effectors of the transformed phenotype.
Lecomte
F
F
Université Libre de Bruxelles, Département de Biologie Moléculaire, Rhode-Saint-Genèse, Belgium.
Szpirer
J
J
Szpirer
C
C
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D016762:Genes, fos; D006801:Humans; D008099:Liver; D051379:Mice; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics; D051381:Rats; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C031016:RPS3A protein, human; D012269:Ribosomal Proteins; C488931:Rps3a protein, rat; C488930:Rsp3a1 protein, mouse; D014409:Tumor Necrosis Factor-alpha