title: Lead-catalysed specific cleavage of ribosomal RNAs.
abstract: Ribosomes have long been known to require divalent metal ions for their functional integrity. Pb2+-induced cleavage of the sugar-phosphate backbone has now been used to probe for metal binding sites in rRNA. Only three prominent Pb2+cleavages have been detected, with cleavage sites 5' of G240 in 16S rRNA and two sites 5' of A505 and C2347 in 23S rRNA. All cleavages occur in non-paired regions of the secondary structure models of the rRNAs and can be competed for by high concentrations of Mg2+, Mn2+, Ca2+ and Zn2+ ions, suggesting that lead is bound to general metal binding sites. Although Pb2+ cleavage is very efficient, ribosomes with fragmented RNAs are still functional in binding tRNA and in peptidyl transferase activity, indicating that the scissions do not significantly alter ribosomal structure. One of the lead cleavage sites (C2347 in 23S RNA) occurs in the vicinity of a region which is implicated in tRNA binding and peptidyl transferase activity. These results are discussed in the light of a recent model which proposes that peptide bond formation might be a metal-catalysed process.
authors.affiliation: Institute of Biochemistry, University of Vienna, Vienna Biocenter, Dr Bohr-Gasse 9/3, A-1030 Vienna, Austria.
authors.forename: D
authors.initials: D
authors.lastname: Winter
authors.forename: N
authors.initials: N
authors.lastname: Polacek
authors.forename: I
authors.initials: I
authors.lastname: Halama
authors.forename: B
authors.initials: B
authors.lastname: Streicher
authors.forename: A
authors.initials: A
authors.lastname: Barta
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D001483:Base Sequence; D001667:Binding, Competitive; D002384:Catalysis; D002413:Cations, Divalent; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007854:Lead / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000378:metabolism*; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D002413:Cations, Divalent; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D007854:Lead; D012343:RNA, Transfer