Emergence of an inhibitor-resistant beta-lactamase (SHV-10) derived from an SHV-5 variant.
An inhibitor-resistant beta-lactamase (SHV-10), derived from an SHV-5 variant (SHV-9), was found in an Escherichia coli clinical isolate. In SHV-10, Ser-130 was replaced by Gly. The enzyme partially retained its ability to hydrolyze penicillins, but its activity against cephalosporins was drastically reduced. A Ser-130-->Gly mutant of the prototype SHV-5, obtained by site-directed mutagenesis, had properties similar to those of SHV-10.
Prinarakis
E E
EE
Department of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
Miriagou
V
V
Tzelepi
E
E
Gazouli
M
M
Tzouvelekis
L S
LS
D000900:Anti-Bacterial Agents / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology*; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000235:genetics; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams; D001618:beta-Lactamases