Hyaluronic acid inhibits interleukin-1-induced superoxide anion in bovine chondrocytes.
OBJECTIVE
To examine the effect of hyaluronic acid (HA) on the induction of superoxide anion by IL-1 in chondrocytes.


METHODS
Bovine articular chondrocytes were treated with different concentrations of IL-1. A chemiluminescent probe (L-012) was added to the medium and chemiluminescence detection was used to measure super oxide anion.


RESULTS
IL-1 caused induction of superoxide anions in a dose-dependent manner. HA (10-100 micrograms/ml) countered superoxide induction caused by 20 ng/ml of IL-1.


CONCLUSIONS
HA can afford protection against cartilage degradation, probably acting as a free-radical scavenger.
Fukuda
K
K
Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka, Japan.
Takayama
M
M
Ueno
M
M
Oh
M
M
Asada
S
S
Kumano
F
F
Tanaka
S
S
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D004353:Drug Evaluation, Preclinical; D006820:Hyaluronic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008163:Luminescent Measurements; D013481:Superoxides / Q000378:metabolism*
D007375:Interleukin-1; D013481:Superoxides; D006820:Hyaluronic Acid