There is not simple method to maintain a constant ethanol concentration in long-term cell culture: keys to a solution applied to the survey of astrocytic ethanol absorption.
Ethanol evaporation from the culture medium is a potential source of misinterpretation of long-term exposure of cells. Different methods have been proposed to prevent this evaporation, the most effective being the saturation of the atmosphere over the culture medium with ethanol. Unfortunately, no simple predictive method has been devised to determine the appropriate concentration of ethanol in the system avoiding either evaporation or contamination of the culture medium. We present some keys to a solution adapted to the culture of astrocytes, which allow for the first time a direct evaluation of ethanol absorption by these cells. The system described remains compatible with normal growth and viability.
Eysseric
H
H
Laboratoire de Pharmacologie et de Toxicologie. CHU de Grenoble, France.
Gonthier
B
B
Soubeyran
A
A
Bessard
G
G
Saxod
R
R
Barret
L
L
D000042:Absorption; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D000431:Ethanol / Q000008:administration & dosage* / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D003470:Culture Media; D000431:Ethanol