Histamine inhibits ATP-induced [Ca2+]i rise through the activation of protein kinase A in HL-60 cells.
We investigated the cross-talk between the histamine and ATP receptors in HL-60 human promyelocytes. While both histamine and extracellular ATP increase intracellular Ca2+ concentration ([Ca2+]i) we found that histamine treatment causes a decrease in the subsequent ATP-induced Ca2+ release from intracellular stores and Ca2+ influx from extracellular space. In addition, histamine also inhibited the subsequent ATP-induced inositol 1.4,5-trisphosphate (IP3) generation in a manner comparable to the Ca2+ release. However, histamine did not inhibit thapsigargin-induced Ca2+ release and influx, thus indicating that histamine does not directly inhibit the Ca2+ release-activated channel (CRAC). Ca2+ elevation induced by 2'- and 3'-O-(4-benzoylbenzoyl) ATP (BzATP), which does not produce IP3, was also inhibited by treatment with histamine, suggesting the presence of ATP-gated channels that are regulated by histamine. Treatment with dibutyryl cAMP or 8-bromo-cAMP inhibited the subsequent ATP-induced response similar to histamine. Moreover, the incubation of cells with N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H89), a protein kinase A inhibitor abolished histamine's inhibitory effect on the ATP-induced [Ca2+]i rise and IP3 formation. These results suggest that histamine inhibits both ATP-induced IP3 production and ATP-activated channel opening, through protein kinase A activation.
Song
S K
SK
Department of Life Science and Basic Science Research Center, Pohang University of Science and Technology, South Korea.
Suh
B C
BC
Lee
H
H
Kim
K T
KT
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004789:Enzyme Activation; D018922:HL-60 Cells; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D015398:Signal Transduction
D006632:Histamine; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D002118:Calcium