Pax-5 is identical to EBB-1/KLP and binds to the VpreB and lambda5 promoters as well as the KI and KII sites upstream of the Jkappa genes.
During B cell differentiation, the pre-B cell stage plays a significant role in immunoglobulin gene rearrangement in the context of the allelic exclusion and kappa chain gene rearrangement. We previously reported that the early B cell-specific binding protein (EBB)-1 transcription factor binds to the promoters of two pre-B cell-specific genes, VpreB and lambda5, and regulates their pre-B cell-specific expression. Here, we demonstrate that EBB-1 binds to the KI and KII sites in the upstream of Jkappa region, which are crucial for kappa chain gene rearrangement. Gene transfer and gel-shift assays demonstrate that EBB-1 is identical to Pax-5 and binds to promoters of VpreB and lambda5 as well as the KI and KII sites. Our results suggest that Pax-5 plays an important role in the coordinate regulation of several immunoglobulin gene family members that are crucial in B cell development.
Tian
J
J
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Okabe
T
T
Miyazaki
T
T
Takeshita
S
S
Kudo
A
A
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001665:Binding Sites; D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D015322:Gene Rearrangement, B-Lymphocyte; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D051757:PAX5 Transcription Factor; D011401:Promoter Regions, Genetic; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D007145:Immunoglobulin kappa-Chains; D009687:Nuclear Proteins; D051757:PAX5 Transcription Factor; C497282:Pax5 protein, mouse; D014157:Transcription Factors