A novel property of adenine nucleotides: sensitivity to helium-neon laser in mitochondrial reactions.
Consideration is made here of the ability of He-Ne laser light to affect both transport and enzymic reactions by acting on substrates. Adenine nucleotides irradiated with 3 Joules/cm2 fluence (10 mW/cm2 fluence rate) showed altered biochemical behaviour when used as substrates for certain mitochondrial reactions in isolated rat liver mitochondria: ADP/ATP antiport and ATP synthase, which allow for oxidative phosphorylation, and adenylate kinase reaction. In order to determine ATP synthase kinetics a specific method was developed which allows for calculation of ADP phosphorylation rate in intact mitochondria. While no change in ATP synthase kinetics was observed as a result of ADP irradiation, adenine nucleotides proved to be sensitive to He-Ne laser irradiation when their interaction with ADP/ATP carrier and adenylate kinase was considered.
Gagliardi
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Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Italy.
Atlante
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Passarella
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D000227:Adenine Nucleotides / Q000378:metabolism / Q000528:radiation effects*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000263:Adenylate Kinase / Q000378:metabolism; D000818:Animals; D006371:Helium; D007834:Lasers; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D009356:Neon; D051381:Rats; D017208:Rats, Wistar
D000227:Adenine Nucleotides; D006371:Helium; D009356:Neon; D000255:Adenosine Triphosphate; D000263:Adenylate Kinase