title: Measurement of protein concentration of aqueous humour in vivo: correlation between laser flare measurements and chemical protein determination.
abstract: We studied the correlation between laser flare cell meter photon count/ms and actual protein concentration both in vitro and in vivo. Laser flare cell meter measurement of human albumin concentrations of 0 to 10 g/l showed photon counts/ms from 0.3 +/- 0.3 to 78.9 +/- 3.9. There was a statistically highly significant linear correlation between photon count/ms and human albumin concentration (r = 0.98, p = 0.0001). Laser flare cell meter measurements were done on 39 cataract patients with the mean age of 77.9 +/- 6.7 years. Aqueous humour obtained by peroperative paracentesis was analysed for total protein. The mean photon count/ms before pupillary dilatation was 11.93 +/- 6.03. There was a significant linear correlation (r = 0.4, p = 0.019) between the photon count/ms after pupillary dilatation (mean 14.73 +/- 12.9, range 2.6-62.4) and anterior chamber protein concentration (mean 0.62 +/- 0.27, range 0.23-1.3 g/l) with the linear regression formula being y = 0.231 x -1.105 where y = log protein concentration (g/l) and x = log of photon count/ms. Laser flare cell meter photon counts/ms may be converted into an equivalent anterior chamber total protein concentration using this formula.
authors.affiliation: Department of Ophthalmology, University of Turku, Finland.
authors.forename: K M
authors.initials: KM
authors.lastname: Saari
authors.forename: O M
authors.initials: OM
authors.lastname: Guillén-Monterrubio
authors.forename: J
authors.initials: J
authors.lastname: Hartikainen
authors.forename: M M
authors.initials: MM
authors.lastname: Hämäläinen
authors.forename: K
authors.initials: K
authors.lastname: Taskinen
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000867:Anterior Chamber / Q000737:chemistry / Q000473:pathology; D001082:Aqueous Humor / Q000737:chemistry / Q000166:cytology; D002386:Cataract / Q000378:metabolism / Q000473:pathology; D002452:Cell Count; D003584:Cytological Techniques; D005136:Eye Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D007834:Lasers; D008297:Male; D008875:Middle Aged; D018918:Phacoemulsification; D012680:Sensitivity and Specificity
chemical_list: D005136:Eye Proteins