title: Monocytes modulate the fibrinolytic balance of endothelial cells.
abstract: Cultured endothelial cells (ECs) produced a constitutive plasminogen activator inhibitor-I (PAI-1), whereas primary culture of monocytes from blood did not produce a detectable amount of PAI-1. Addition of monocytes to ECs caused the accumulation of a large amount of PAI-1 in the supernatant in a dose- and time-dependent manner. Having almost no effect on the production of tissue-type plasminogen activator (t-PA), monocytes decreased the potential fibrinolytic activity of ECs. The 6 hours conditioned medium obtained from the coculture system between monocytes and either ECs or paraformaldehyde-fixed ECs had almost the same effect on the other ECs to produce PAI-1 and t-PA as monocytes that were direct contact with ECs. In addition, this effect was specifically inhibited by using two antibodies against interleukin-1 beta and tumor necrosis factor-alpha. These results indicate that interleukin-1 beta and tumor necrosis factor-alpha induced by the coculture are mostly responsible for decreasing the fibrinolytic activity of ECs.
authors.affiliation: Department of cardiology, Institute of Hematology, Jichi Medical School, Tochigi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Funayama
authors.forename: Y
authors.initials: Y
authors.lastname: Sakata
authors.forename: S
authors.initials: S
authors.lastname: Kitagawa
authors.forename: U
authors.initials: U
authors.lastname: Ikeda
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: J
authors.initials: J
authors.lastname: Masuyama
authors.forename: J
authors.initials: J
authors.lastname: Mimuro
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Shimada
mesh_terms: D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000378:metabolism*; D005342:Fibrinolysis; D006801:Humans; D007958:Leukocyte Count; D009000:Monocytes / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D017395:Plasminogen Activator Inhibitor 1; D010959:Tissue Plasminogen Activator