Expression of thromboxane synthase in kidney tissues from patients with IgA nephropathy.
Thromboxane A2 (TXA2) is a potent vasoconstrictor which is known to be involved in the pathogenesis of experimental glomerulonephritis, although its exact pathogenic significance is not clear in human glomerulonephritis. We have investigated the expression of thromboxane synthase (TXS) in kidney tissues from patients with IgA nephropathy (IgAN), using RT-PCR and immunohistochemical methods. Biopsied renal tissues from thirty-four patients with IgAN (24 whole tissues and 10 isolated glomeruli) and normal renal tissues from 11 nephrectomized kidneys (control, eight whole tissues and three isolated glomeruli) were included in this study. TXS mRNA expression was observed in 10 out of 24 (42%) whole tissue specimens from IgAN, but no such message was disclosed in the control tissue. There was no detectable TXS mRNA expression in the isolated glomeruli either from IgAN patients or controls, although constant mRNA expressions for GAPDH and VPF/VEGF were observed. IgAN patients with positive TXS mRNA had significantly reduced GFR with elevated serum creatinine and serum beta 2-microglobulin levels. Immunostaining, using a monoclonal anti-TXS antibody, identified the localization of the TXS protein in the interstitial areas where monocyte/macrophage infiltration was abundant, as well as in the arterioles of the kidney tissues with advanced tissue damage. It was concluded that TXA2 produced by the interstitial infiltrating cells during the inflammatory process might be involved in the progression of IgA nephropathy.
Endoh
M
M
Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa, Japan.
Kashem
A
A
Yamauchi
F
F
Yano
N
N
Nomoto
Y
Y
Sakai
H
H
Kurokawa
K
K
D003404:Creatinine / Q000097:blood; D005922:Glomerulonephritis, IGA / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D007678:Kidney Glomerulus / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013928:Thromboxane A2 / Q000502:physiology; D013930:Thromboxane-A Synthase / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000032:analysis
D012333:RNA, Messenger; D001613:beta 2-Microglobulin; D013928:Thromboxane A2; D003404:Creatinine; D013930:Thromboxane-A Synthase