title: A comparison of febrile responses induced by LPS from E. coli and S. abortus in unrestrained rats placed in a thermal gradient.
abstract: The purpose of our study was a comparison of pyrogenic and behavioural effects of Escherichia coli (E.coli) and Salmonella abortus (S.abortus) endotoxins in unrestrained, freely moving Wistar rats, placed in a thermal gradient and having an easy access to ambient temperatures within a range 5-40 degrees C. Hypothalamic and chosen by the rats ambient temperatures as well as locomotor activity were recorded before and after intraperitoneal injection of 1 mg/kg lipopolysaccharide from E. coli or from S. abortus. Control animals were injected with pyrogen-free saline. Both endotoxins induced warm-seeking behaviour which was accompanied by biphasic fever. Locomotor activity of LPS-injected rats was reduced. S. abortus-induced fever peaked at 100th minute (reaching 37.5 +/- 0.2 degrees C) and at 250th minute (reaching 38.1 +/- 0.1 degrees C). Respective data for E. coli fever were: 170th minute (when hypothalamic temperature reached 37.6 +/- 0.3 degrees C) and 430th minute (with hypothalamic temperature of 38.6 +/- 0.1 degrees C). Comparing to S. abortus-generated fever both peaks of E. coli LPS-induced fever were significantly delayed (p < 0.05). A limited structural variability of lipid A from both bacteria is likely to be responsible for the difference in fever timing recorded in this study.
authors.affiliation: Department of Physiology, University School of Medical Sciences, Bydgoszcz.
authors.forename: M
authors.initials: M
authors.lastname: Bodurka
authors.forename: M
authors.initials: M
authors.lastname: Caputa
authors.forename: J
authors.initials: J
authors.lastname: Bodurka
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D001833:Body Temperature Regulation / Q000187:drug effects*; D004731:Endotoxins / Q000633:toxicity*; D004768:Enterotoxins / Q000633:toxicity*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005334:Fever / Q000139:chemically induced* / Q000503:physiopathology / Q000523:psychology; D006358:Hot Temperature; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012475:Salmonella / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001427:Bacterial Toxins; D004731:Endotoxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027952:salmonella toxin; C027538:heat-labile enterotoxin, E coli