title: Preparation of surface-modified albumin nanospheres.
abstract: Surface-modified human serum albumin (HSA) nanospheres with a size of around 100 nm in diameter were prepared from poly(amidoamine)-poly(ethylene glycol) copolymer grafted human serum albumin (HSA-PAA-PEG) and poly(thioetheramido acid)-poly(ethylene glycol) copolymer grafted human serum albumin (HSA-PTAAC-PEG). The nanospheres were produced using a pH-coacervation method and cross-linked with glutaraldehyde. The cross-linking efficiency was affected by the type of albumin conjugate used. The zeta potential of the surface-modified nanospheres was significantly lower than that of unmodified particles. The existence of a hydrated steric barrier surrounding the nanospheres was confirmed by electrolyte- and pH-induced flocculation tests. The surface-modified nanospheres showed a reduced plasma protein adsorption on the particle surface compared with unmodified particles.
authors.affiliation: Department of Pharmaceutical Sciences, University of Nottingham, UK.
authors.forename: W
authors.initials: W
authors.lastname: Lin
authors.forename: M C
authors.initials: MC
authors.lastname: Garnett
authors.forename: M C
authors.initials: MC
authors.lastname: Davies
authors.forename: F
authors.initials: F
authors.lastname: Bignotti
authors.forename: P
authors.initials: P
authors.lastname: Ferruti
authors.forename: S S
authors.initials: SS
authors.lastname: Davis
authors.forename: L
authors.initials: L
authors.lastname: Illum
mesh_terms: D000327:Adsorption; D001798:Blood Proteins; D004337:Drug Carriers; D004573:Electrolytes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D008863:Microspheres; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D012709:Serum Albumin / Q000138:chemical synthesis / Q000737:chemistry*; D000075462:Serum Albumin, Human; D013499:Surface Properties
chemical_list: D001798:Blood Proteins; D004337:Drug Carriers; D004573:Electrolytes; D012709:Serum Albumin; C106417:human serum albumin-poly(amidoamine)-poly(ethylene glycol) copolymer; C106418:human serum albumin-poly(thioetheramido acid)-poly(ethylene glycol) copolymer; D011092:Polyethylene Glycols; D000075462:Serum Albumin, Human