title: Advances in gene targeting methods.
abstract: Gene targeting in embryonic stem cells is commonly used for gene inactivation and the generation of mouse mutants. The combined use of methods for site-specific and homologous DNA recombination expands the potential of gene targeting in embryonic stem cells considerably and offers the opportunity of conditional gene targeting in mice.
authors.affiliation: Institute for Genetics, Department for Immunology, Cologne, Germany. rkuehn@gen1.genetik.uni-koeln.de
authors.forename: R
authors.initials: R
authors.lastname: Kühn
authors.forename: F
authors.initials: F
authors.lastname: Schwenk
mesh_terms: D000818:Animals; D018390:Gene Targeting / Q000379:methods* / Q000639:trends*; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics