title: Interleukin-12 and tumor necrosis factor-alpha levels in cerebrospinal fluid of multiple sclerosis patients.
abstract: Concentrations of interleukin (IL)-12 and tumor necrosis factor-alpha (TNF-alpha) in cerebrospinal fluid (CSF) were measured in patients with multiple sclerosis (MS) and control patients with non-inflammatory neurological diseases (NIND) by an enzyme-linked immunosorbent assay. TNF-alpha was detectable in the CSF of 60% of the patients with active MS, none of those with inactive MS and 29% of patients with NIND. CSF concentrations of TNF-alpha correlated with the degree of disability in MS patients (P < 0.05). Detectable levels of IL-12 were found in 10% of the MS CSF samples and 18% of NIND CSF samples. There was a significant relationship between CSF concentrations of IL-12 and those of TNF-alpha in MS patients (P < 0.05); no relationship was observed between the presence of IL-12 and disease activity or severity. These findings further stress the involvement of T helper 1 type-response within the central nervous system in MS.
authors.affiliation: Institute of Neurology, Clinical Center of Serbia, Belgrade, Yugoslavia.
authors.forename: J
authors.initials: J
authors.lastname: Drulović
authors.forename: M
authors.initials: M
authors.lastname: Mostarica-Stojković
authors.forename: Z
authors.initials: Z
authors.lastname: Lević
authors.forename: N
authors.initials: N
authors.lastname: Stojsavljević
authors.forename: V
authors.initials: V
authors.lastname: Pravica
authors.forename: S
authors.initials: S
authors.lastname: Mesaros
mesh_terms: D000328:Adult; D003627:Data Interpretation, Statistical; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000134:cerebrospinal fluid*; D008297:Male; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid; D014409:Tumor Necrosis Factor-alpha / Q000134:cerebrospinal fluid*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12