Conservation of the multidrug resistance efflux gene oprM in Pseudomonas aeruginosa.
An intragenic probe derived from the multidrug resistance gene oprM hybridized with genomic DNA from all 20 serotypes of Pseudomonas aeruginosa and from all 34 environmental and clinical isolates tested, indicating that the MexA-MexB-OprM multidrug efflux system is highly conserved in this organism. The oprM probe also hybridized with genomic DNA from Pseudomonas aureofaciens, Pseudomonas chlororaphis, Pseudomonas syringae, Burkholderia pseudomallei, and Pseudomonas putida, suggesting that efflux-mediated multidrug resistance mechanisms may be somewhat broadly distributed.
Bianco
N
N
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Neshat
S
S
Poole
K
K
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005798:Genes, Bacterial; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial