title: Antibodies against inner-ear proteins in the sera of patients with inner-ear diseases.
abstract: Sera from patients with various inner-ear diseases, especially Ménière's disease, were investigated by Western blot against guinea pig inner-ear proteins. Of 45 patients, 24 (53%) with various inner-ear diseases had antibodies against inner-ear proteins, compared with 0 of 10 (0%) in control subjects without inner-ear diseases. Of the 10 proteins that showed a positive reaction with patient sera, the 28-kD band was unique in that it appeared only in the membranous fraction of the inner ear and was highly positive (28%) in reaction with Ménière's disease patient sera. The results in the present study with proteins extracted from guinea pig inner ear were consistent with our previous study using proteins from human inner ear, suggesting that the 28-kD protein may be a candidate for detecting autoimmune inner-ear disease.
authors.affiliation: Department of Otolaryngology, Shiga University of Medical Science, Seta, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: M S
authors.initials: MS
authors.lastname: Krug
authors.forename: K C
authors.initials: KC
authors.lastname: Cheng
authors.forename: Y
authors.initials: Y
authors.lastname: Yazawa
authors.forename: J
authors.initials: J
authors.lastname: Bernstein
authors.forename: T J
authors.initials: TJ
authors.lastname: Yoo
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000175:diagnosis; D015153:Blotting, Western; D016022:Case-Control Studies; D007758:Ear, Inner / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006311:Hearing Disorders / Q000276:immunology; D006319:Hearing Loss, Sensorineural / Q000276:immunology; D006801:Humans; D007759:Labyrinth Diseases / Q000276:immunology*; D008297:Male; D008575:Meniere Disease / Q000276:immunology; D010040:Otosclerosis / Q000276:immunology; D011506:Proteins / Q000032:analysis / Q000145:classification / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate; D014012:Tinnitus / Q000276:immunology
chemical_list: D001323:Autoantibodies; D011506:Proteins; D012967:Sodium Dodecyl Sulfate