title: ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein.
abstract: We have identified and characterized a novel murine gene, Ectoderm-Neural Cortex-1 (ENC-1), that is an early and highly specific marker of neural induction in vertebrates. ENC-1, which encodes a kelch family related protein, is expressed during early gastrulation in the prospective neuroectodermal region of the epiblast and later in development throughout the nervous system (NS). ENC-1 expression is highly dynamic and, after neurulation, preferentially defines prospective cortical areas. The only apparent expression of ENC-1 outside the NS is restricted to the rostral-most somitomere of the presomitic mesoderm, at the times corresponding to the epithelialization that precedes somite formation. Cellular expression of epitope-tagged ENC-1 shows extensive co-localization of ENC-1 with the actin cytoskeleton, and immunoprecipitation studies demonstrate a physical association between ENC-1 and actin. ENC-1 functions as an actin-binding protein that may be important in the organization of the actin cytoskeleton during neural fate specification and development of the NS.
authors.affiliation: Preuss Laboratory of Molecular Neuro-Oncology and Department of Neurological Surgery, University of California, San Francisco, California 94143, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Hernandez
authors.forename: P J
authors.initials: PJ
authors.lastname: Andres-Barquin
authors.forename: S
authors.initials: S
authors.lastname: Martinez
authors.forename: A
authors.initials: A
authors.lastname: Bulfone
authors.forename: J L
authors.initials: JL
authors.lastname: Rubenstein
authors.forename: M A
authors.initials: MA
authors.lastname: Israel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015870:Gene Expression / Q000235:genetics; D005796:Genes / Q000235:genetics*; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009420:Nervous System / Q000378:metabolism*
chemical_list: D008840:Microfilament Proteins