[The effect of various physiological and genetic factors on the transition process of enterotoxigenic strains of Escherichia coli into uncultured state].
Factors accelerating the transfer of enterotoxigenic E. coli strains in unculturable state have been revealed. These factors were illumination, aeration, hypertonic conditions of the microcosm, and exposure to cobalt chloride. Enterotoxigenic E. coli cells were seeded in solid nutrient media from microcosms with 0.9% NaCl solution for the longest time. The rate of transfer in unculturable forms of the examined bacteria changed depending on the medium of preliminary culturing and the phase of culture growth. Effects of mutations in some chromosome genes on the rate of transfer of bacteria in unculturable state were studied. Switching off the chromosomal genes gltB, livJ, and ompR accelerated the transfer of bacteria in unculturable forms. The vegetative functions of uncultured forms of the model enterotoxigenic strain IG187 recover in the presence of low concentrations of streptomycin or chloramphenicol.
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D015169:Colony Count, Microbial; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D005798:Genes, Bacterial; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D014871:Water Microbiology
D004768:Enterotoxins; D015345:Oligonucleotide Probes
Vliianie nekotorykh fiziologicheskikh i geneticheskikh faktorov na protsess perekhoda énterotoksigennykh shtammov Escherichia coli v nekul'tiviruemoe sostoianie.