Effects of 3-phenylamino-1,2-propanediol and its mono- and dioleylesters on the production of reactive oxygen metabolites in human polymorphonuclear leukocytes.
Human polymorphonuclear leukocytes (PMNL) were exposed to 3-phenylamino-1,2-propanediol (PAP) or to its mono- or dioleylesters. The dioleylester of PAP (140 microM) slightly increased the production of reactive oxygen metabolites (ROM) from 8 +/- 2 mV to 18 +/- 3 mV, whereas PAP and its mono-oleylester were without any effect on ROM production in PMNL. None of the compounds were able to modulate formyl-Methionyl-Leucyl-Phenylalanine-induced production of ROM. Moreover, PAP did not have any effect on the production of ROM induced by phorbol myristate acetate (PMA). On the other hand, both mono- and dioleylesters of PAP dose-dependently inhibited PMA-induced production of ROM. The dioleylester of PAP, at a concentration of 14 microM, inhibited PMA-induced production of ROM by 79%, whereas a 2.3 mM concentration of mono-oleylester of PAP was required for 65% inhibition of PMA-induced production of ROM. Moreover, both esters of PAP shifted the peak production of ROM by PMA to a later time-point. Our results suggest that mono- and dioleylesters of PAP modulate the responses of PMNL upon stimulation with PMA, whereas PAP does not have any effect on PMA-induced ROM production in PMNL.
Heiskanen
K M
KM
National Public Health Institute, Department of Environmental Medicine, Kuopio, Finland. Kaisa.Heiskanen@ktl.fi
Savolainen
K M
KM
D000704:Analysis of Variance; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004952:Esters / Q000633:toxicity; D006801:Humans; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011409:Propylene Glycols / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage / Q000633:toxicity
D004952:Esters; D011409:Propylene Glycols; D017382:Reactive Oxygen Species; C077151:3-phenylamino-1,2-propanediol; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013755:Tetradecanoylphorbol Acetate