Development of a novel octadecyl-bonded silica column and evaluation of its reliability in chromatographic analysis.
The chromatographic characterization of an octadecyl-bonded silica (ODS) column for high-performance liquid chromatography is described. In general, columns of the same type but obtained from various manufacturers give different chromatographic results, due to differences in the purity of column packings, the properties of the silica gel supports and the density of silanols on the surface of the silica gel. In order to solve this problem and to obtain a high performance ODS column, a series of methods with different samples and conditions were evaluated. The result is important for the optimization of conditions for the synthesis of ODS packings and for minimizing the deleterious effects arising from adsorption activity and metal impurity.
Tanaka
H
H
GL Sciences, Saitama, Japan.
Kamada
M
M
Nyudo
M
M
Ohira
M
M
D019342:Acetic Acid / Q000032:analysis / Q000737:chemistry; D018926:Anti-Allergic Agents / Q000032:analysis; D000889:Anti-Arrhythmia Agents / Q000032:analysis; D001462:Barbital / Q000032:analysis; D002744:Chlorpheniramine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003300:Copper / Q000032:analysis / Q000737:chemistry; D005561:Formates / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006993:Hypnotics and Sedatives / Q000032:analysis; D015125:Oxyquinoline / Q000032:analysis / Q000737:chemistry; D010316:Particle Size; D010575:Pesticides / Q000032:analysis / Q000737:chemistry; D019800:Phenol; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D011342:Procainamide / Q000032:analysis; D011725:Pyridines / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties
D018926:Anti-Allergic Agents; D000889:Anti-Arrhythmia Agents; D005561:Formates; D006993:Hypnotics and Sedatives; D010575:Pesticides; D010636:Phenols; D011725:Pyridines; C030544:formic acid; D019800:Phenol; D002744:Chlorpheniramine; D015125:Oxyquinoline; D001462:Barbital; D012822:Silicon Dioxide; D003300:Copper; D011342:Procainamide; C023666:pyridine; D019342:Acetic Acid