title: Genomic MHC haplotyping of mutant cell lines using a novel marker.
abstract: The aim of this study was to characterize MHC mutant cell lines by studying haplospecific markers within the MHC and specifically in the 250 kilobase (kb) region between the HLA B and TNF loci. This region has been difficult to define because of the lack of appropriate markers. Spontaneous MHC mutants were isolated after immunoselection with an anti-HLA A2 monoclonal antibody and complement. Ten mutants were characterized using serological or allelic and genomic DNA markers within the HLA A to HLA DQ region of the MHC. Most mutants lost at least the 3 megabases of DNA from HLA A to HLA DQ viz the whole haplotype carrying HLA A2. Variants which have lost either HlA A alone or HLA A and HLA B were also found. The results show that it is possible to map the extent of the deletion between HLA B and TNF. Haplospecific scanning patterns for the CL region appear particularly useful.
authors.affiliation: Department of Clinical Immunology, Royal Perth Hospital, Western Australia.
authors.forename: M A
authors.initials: MA
authors.lastname: Degli-Esposti
authors.forename: J F
authors.initials: JF
authors.lastname: Williamson
authors.forename: G K
authors.initials: GK
authors.lastname: Tay
authors.forename: B
authors.initials: B
authors.lastname: Salmon
authors.forename: G A
authors.initials: GA
authors.lastname: Joseph
authors.forename: A A
authors.initials: AA
authors.lastname: Morley
authors.forename: R L
authors.initials: RL
authors.lastname: Dawkins
mesh_terms: D002460:Cell Line; D017353:Gene Deletion; D005819:Genetic Markers; D015894:Genome, Human; D006680:HLA Antigens / Q000235:genetics; D006239:Haplotypes; D006650:Histocompatibility Testing; D006801:Humans; D008285:Major Histocompatibility Complex; D005810:Multigene Family; D009154:Mutation; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D005819:Genetic Markers; D006680:HLA Antigens; D014409:Tumor Necrosis Factor-alpha