title: Activation of a Ca(2+)-dependent protein kinase in response in heat shock in the myxoamoebae of a true slime mold, Physarum polycephalum.
abstract: Protein kinase activities in myxoamoebae of a true slime mold, Physarum polycephalum, were investigated in response to heat shock. In-gel assay detected an apparent activation of a Ca(2+)-dependent, 53-kDa protein kinase that phosphorylated casein but not histone H1. This enzyme needed co-presence of Mg2+ ion with Ca2+ for activity. Treatment with calf intestinal alkaline phosphatase did not affect the heat-inducible 53-kDa protein kinase activity at all. The effects of protein kinase inhibitors were examined, and staurosporine suppressed the activity of this enzyme completely. H-7 decreased the activity to about 20% and HA-1004 to 65%. These results suggest that this protein kinase that may phosphorylate tyrosine and serine/threonine residues of target proteins is activated by heat shock in Physarum cells, and the activation is not regulated via phosphorylation by putative protein kinase(s) that may act at an upstream position in the signaling cascade(s).
authors.affiliation: Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Maruya
authors.forename: M
authors.initials: M
authors.lastname: Mitsui
authors.forename: K
authors.initials: K
authors.lastname: Murakami-Murofushi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium; D004789:Enzyme Activation; D006361:Heating; D008274:Magnesium; D016839:Physarum polycephalum / Q000201:enzymology* / Q000254:growth & development; D011494:Protein Kinases / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D000469:Alkaline Phosphatase; D002118:Calcium; D008274:Magnesium; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases