title: A study of diffusion isotropy in single neurons by using NMR microscopy.
abstract: The apparent diffusion coefficient (ADC) of water was measured in single Aplysia californica neurons by using NMR microscopy encoded in each of two perpendicular gradient directions. Comparisons of the mean ADCs of the gross nuclear and cytoplasmic compartments in five cells, and 50 subregions within these cells, showed no significant difference between the diffusion measurements in the majority of cases. Since anisotropic diffusion would make the ADC dependent on the encoding direction, the results indicate that the ADC in these single neurons is isotropic at the spatial and temporal resolutions used in these studies. Consequently, a single scalar ADC measurement is sufficient for characterizing the ADC in these cells, hence reducing the acquisition time and measurement complexity that would have been required had the ADC been anisotropic.
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: E W
authors.initials: EW
authors.lastname: Hsu
authors.forename: N R
authors.initials: NR
authors.lastname: Aiken
authors.forename: S J
authors.initials: SJ
authors.lastname: Blackband
mesh_terms: D000818:Animals; D016880:Anisotropy; D001048:Aplysia; D004058:Diffusion; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water