Intragastric immunization of rats with Entamoeba histolytica trophozoites induces cecal mucosal IgE, eosinophilic infiltration, and type I hypersensitivity.
We have investigated the role of IgE in the local immunity of intestinal amebiasis, a parasitic infection known to induce specific antibody-forming cells (AFC) and IgA antibodies in rodents and humans. We found that intragastric immunization of rats with glutaraldehyde-fixed Entamoeba histolytica trophozoites significantly increased antiameba AFC in the Peyer's patches and spleen and that the lamina propria of the cecum from immunized animals was infiltrated by eosinophils armed with IgE antibodies. Morphometric analysis showed that IgE-containing cells and eosinophils were nearly three times more abundant in the cecum of immunized rats. Antigenic challenge with amebal lysates provoked an increase in the short-circuit current and in the transepithelial potential difference in Ussing-chambered cecum preparations from immunized rats. Although eosinophilia and the increase of IgE are common consequences of infection by parasitic worms, our results indicate that local immunity in intestinal amebiasis also involves IgE deposition, eosinophil infiltration, and type I hypersensitivity, which may explain some symptoms of amebic dysentery such as colic, abdominal tension, tenesmus, and bloody stools.
Navarro-García
F
F
Department of Cell Biology, CINVESTAV-IPN, México D.F., Mexico.
López-Revilla
R
R
Vega-López
M A
MA
Domínguez-Robles
M C
MC
Enríquez-Rincón
F
F
Tsutsumi
V
V
D000562:Amebiasis / Q000209:etiology / Q000276:immunology; D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology; D000953:Antigens, Protozoan / Q000276:immunology*; D002432:Cecum / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004594:Electrophysiology; D004748:Entamoeba histolytica / Q000276:immunology*; D004802:Eosinophilia / Q000276:immunology*; D005753:Gastric Mucosa / Q000276:immunology*; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007114:Immunization; D007073:Immunoglobulin E / Q000502:physiology*; D007263:Infusions, Parenteral; D007413:Intestinal Mucosa / Q000276:immunology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000953:Antigens, Protozoan; D007073:Immunoglobulin E