Large-scale production of class I bound peptides: assigning a signature to HLA-B*1501.
A peptide-based vaccine must be bound and presented by major histocompatibility complex class I molecules to elicit a CD8(+) T-cell response. Because class I HLA molecules are highly polymorphic, it has yet to be established how well a vaccine peptide that stimulates one individual's CD8(+) cytotoxic T lymphocytes will be presented by a second individual's different class I molecules. Therefore, to facilitate precise comparisons of class I peptide binding overlaps, we uniquely combined hollow-fiber bioreactors and mass spectrometry to assign precise peptide binding signatures to individual class I HLA molecules. In applying this strategy to HLA-B*1501, we isolated milligram quantities of B*1501-bound peptides and mapped them using mass spectrometry. Repeated analyses consistently assign the same peptide binding signature to B*1501; the degree of peptide binding overlap between any two class I molecules can thus be determined through comparison of their peptide signatures.
Prilliman
K
K
Department of Microbiology & Immunology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, OK 73190, USA.
Lindsey
M
M
Zuo
Y
Y
Jackson
K W
KW
Zhang
Y
Y
Hildebrand
W
W
D003602:Cytotoxicity, Immunologic; D015235:HLA-B Antigens / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding; D014614:Vaccines, Synthetic / Q000276:immunology*
D015235:HLA-B Antigens; D010455:Peptides; D014614:Vaccines, Synthetic