title: Sensing of DNA non-homology lowers the initiation of meiotic recombination in yeast.
abstract: BACKGROUND
  Meiotic recombination between homologous chromosomes in the yeast Saccharomyces cerevisiae is initiated by the formation of DNA double-strand breaks (DSBs). The mechanism of DSB formation and the factors that determine their frequency and location have yet to be elucidated. Current studies of meiotic recombination are also concerned with the question of the functional relationship between DSB formation and the other meiotic processes of homology searching, pairing and synapsis of homologues.
  
  
  RESULTS
  To test if DNA identity is required for high levels of DSBs and recombination, we have asked whether small DNA heterologies (140-547 bp) located within the well characterized ARG4 initiator of meiotic recombination, can affect DSB formation and gene conversion events in the ARG4 locus. The present physical and genetic analyses show that some heterologies reduced recombination frequencies without altering DSB formation, whereas others reduced both DSB and gene conversion frequencies.
  
  
  CONCLUSIONS
  These results suggest that DNA heterologies overlapping a recombination initiator impair meiotic gene conversion at two levels. First, some heterologies affect the level of DSB formation, revealing the existence of an anti-initiation process sensing the presence of sequence non-homology between the homologous chromosomes. Second, heterologies can impair the successful processing of the recombination intermediates once DSBs are made. We present a model for interhomologue cross-talks involving chromosomal and DNA/DNA interactions.
authors.affiliation: Institut Curie, UMR144 CNRS, Compartimentation et Dynamique Cellulaires, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Rocco
authors.forename: A
authors.initials: A
authors.lastname: Nicolas
mesh_terms: D001123:Argininosuccinate Lyase; D015825:Chromosomes, Fungal / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D004171:Diploidy; D005656:Fungal Proteins / Q000235:genetics; D005785:Gene Conversion; D005819:Genetic Markers; D006579:Heterozygote; D006720:Homozygote; D008540:Meiosis / Q000235:genetics*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D005819:Genetic Markers; D029701:Saccharomyces cerevisiae Proteins; C087900:Arg4 protein, S cerevisiae; D001123:Argininosuccinate Lyase