Chromosomal changes observed in immortalized human keratinocytes transformed by ionizing radiation.
Human epithelial cancer cells were induced by concerted action of DNA tumor virus and X-ray radiation. Treatment of nontumorigenic early passage AD12-SV40 immortalized epithelial cells (RHEK-1) at passage 23 with radiation, resulted in further changes in their growth properties. One day old cultures of these RHEK-1 cells were irradiated with graded doses of X-rays (0, 2, 4, 6, and 8 Gy i.e. RHEK-1, RHEK-1/200R, RHEK-1/400R, RHEK-1/600R, and RHEK-1/800R). Morphologic alterations, the ability to grow in soft agar, and to form rapidly-growing squamous cell carcinomas in nude mice were concomitantly acquired properties of the radiation transformed cell lines RHEK-1/200R and RHEK-1/ 400R. On the basis of commonality in having addition of some extra material in chromosome 11 in the region between q14/q22 in all tumorigenic cell lines RHEK-1/200R and RHEK-1/400R, and deletion of the same region in nontumorigenic irradiated cell lines-RHEK-1/600R and RHEK-1/800R, it is deduced this region may have some important oncogene/s or other gene/s that play an important role in tumorigenesis. When compared to squamous cell carcinoma data, the duplication observed in the present study is also observed in 28 to 38% of head and neck and also in 25% of cases of untreated malignant lesions of oral squamous cell carcinoma. Thus, this study shows the correlation between in vitro induced squamous cell carcinoma to in vivo tumors.
Hukku
B
B
Children's Hospital of Michigan, Department of Pediatrics, Detroit 48201, USA.
Thraves
P
P
Dritschilo
A
A
Rhim
J S
JS
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002461:Cell Line, Transformed; D002869:Chromosome Aberrations / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015603:Keratinocytes / Q000473:pathology / Q000528:radiation effects*; D051379:Mice; D008819:Mice, Nude; D014178:Translocation, Genetic