title: Flow cytometry and immunoblotting analysis of monoclonal antibodies directed to complement regulatory proteins.
abstract: A series of 18 monoclonal antibodies directed to complement regulatory proteins were investigated by flow cytometry and immunoblotting. Seventeen antibodies are directed against a phosphatidyl inositol-linked glycoprotein since they show a dual population of erythrocytes from a paroxysmal nocturnal haemoglobinuria (PNH) patient. From this group, 6 antibodies revealed a 65-70 kDa band on immunoblots allowing their identification as anti-DAF (Decay Accelerating Factor; CD55 antigen), and 11 bound to a 20 kDa molecule corresponding to MIRL (Membrane Inhibitor of Reactive lysis, CD59 antigen). One antibody revealed an homogeneous population from the PNH patient and bound to a 200 kDa band on immunoblot that might corresponds to the CR1 (Complement Receptor type 1; CD35).
authors.affiliation: Etablissement de Transfusion Sanguine de Loire Atlantique/Vendée, Site de Nantes, France.
authors.forename: D
authors.initials: D
authors.lastname: Blanchard
authors.forename: J M
authors.initials: JM
authors.lastname: Navenot
authors.forename: Y
authors.initials: Y
authors.lastname: Petit-Le Roux
authors.forename: C
authors.initials: C
authors.lastname: Willem
authors.forename: M J
authors.initials: MJ
authors.lastname: Loirat
mesh_terms: D000911:Antibodies, Monoclonal; D018958:CD55 Antigens / Q000276:immunology*; D018957:CD59 Antigens / Q000276:immunology*; D016022:Case-Control Studies; D004912:Erythrocytes / Q000276:immunology*; D005434:Flow Cytometry; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D017463:Receptors, Complement 3b / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D018958:CD55 Antigens; D018957:CD59 Antigens; D017463:Receptors, Complement 3b