title: Determination of lysergide in urine by high-performance liquid chromatography combined with electrospray ionisation mass spectrometry.
abstract: A quantitative method which avoids derivatisation is described for the determination of lysergide (LSD) levels in urine. Sample preparation included addition of methysergide as an internal standard followed by solid-phase extraction. LSD was analysed on a system consisting of a C18 stationary phase and a mobile phase of 0.1 M acetate buffer pH 8.0-acetonitrile-triethylamine (75:25:0.25, v/v). LSD was detected by electrospray ionisation mass spectrometry with selected ion monitoring. The quantification limit was 0.5 ng/ml and the method was linear up to 10 ng/ml of LSD in urine.
authors.affiliation: Laboratory of the Government Chemist, Teddington, Middlesex, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: White
authors.forename: T
authors.initials: T
authors.lastname: Catterick
authors.forename: M E
authors.initials: ME
authors.lastname: Harrison
authors.forename: D E
authors.initials: DE
authors.lastname: Johnston
authors.forename: G D
authors.initials: GD
authors.lastname: Reed
authors.forename: K S
authors.initials: KS
authors.lastname: Webb
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008238:Lysergic Acid Diethylamide / Q000652:urine*
chemical_list: D008238:Lysergic Acid Diethylamide