Capillary electrophoresis of cytokinins and cytokinin ribosides.
A CE separation of cytokinins and cytokinin ribosides and some other purine and pyrimidine bases has been developed. Two electrolyte systems have been tested: 150 mM phosphoric acid, pH 1.8 and 50 mM sodium dodecylsulphate + 20 mM borate, pH 9.2. The migration times were measured and the effects of the solute structures were discussed. Preliminary experiments with plant extracts have been performed to identify the cytokinins and their ribosides. Both the systems can be used, but 150 mM phosphoric acid is better suited for identification of cytokinins in plant extracts, as the electropherograms are subject to fewer interferences.
Pacáková
V
V
Department of Analytical Chemistry, Charles University, Prague, Czech Republic.
Stulík
K
K
Vlasáková
V
V
Brezinová
A
A
D003583:Cytokinins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D012263:Ribonucleosides / Q000032:analysis*
D003583:Cytokinins; D012263:Ribonucleosides