Identification of pig primordial germ cells by immunocytochemistry and lectin binding.
Monoclonal antibodies anti-SSEA-1 and EMA-1, and the lectins DBA and LTA, bound to the surface of large, round cells randomly distributed in the 26-day pig genital ridge. Other antibodies, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81, did not react with any cells in the pig genital ridge. SSEA-1-positive cells displayed pseudopods and appeared to migrate from the dorsal mesentery of the hindgut (18-day) to the primordium of the gonad (day 23) and entered the genital ridge by 26 days. The number of SSEA-1-positive cells associated with the dorsal mesentery and genital ridge markedly increased from the 18-day to the 26-day pig embryo. It was concluded that the SSEA-1-positive cells were primordial germ cells (PGCs). Using these markers and alkaline phosphatase histochemistry, pig PGCs derived from the 26-day genital ridge showed no proliferation when grown in STO co-culture in the presence of human LIF, bFGF and SCF.
Takagi
Y
Y
Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan.
Talbot
N C
NC
Rexroad
C E
CE
Pursel
V G
VG
D000818:Animals; D000911:Antibodies, Monoclonal; D019070:Cell Lineage; D004622:Embryo, Mammalian / Q000166:cytology*; D005854:Germ Cells / Q000166:cytology*; D007150:Immunohistochemistry; D037102:Lectins; D051379:Mice; D013552:Swine
D000911:Antibodies, Monoclonal; D037102:Lectins