Expression of intercellular adhesion molecule-1 and lymphocyte function-associated antigen-1 on alveolar macrophages in the acute stage of radiation-induced lung injury in rats.
We investigated the expression of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) on alveolar macrophages and on lung tissue in the early stage of radiation-induced lung injury. Cells in the bronchoalveolar lavage and lung tissue were obtained from rats at various times between 1 and 8 weeks after 20 Gy of 60Co gamma irradiation of a hemithorax. These specimens were stained immunohistochemically with anti-ICAM-1 and anti-LFA-1alpha monoclonal antibodies. The expression of these factors was compared with that of a control group. The total number of alveolar macrophages in the bronchoalveolar lavage was significantly reduced from 1 to 3 weeks, and the number of neutrophils was significantly increased 2 and 3 weeks after irradiation. ICAM-1 and LFA-1 expression on alveolar macrophages was significantly increased starting 1 week after irradiation. The expression of ICAM-1 and LFA-1 on lung tissue was not elevated up to 8 weeks after irradiation. In conclusion, the increased expression of ICAM-1 and LFA-1 on alveolar macrophages as early as 1 week after irradiation suggests that adhesion molecules play a role in the development of radiation-induced lung injury.
Kawana
A
A
Department of Internal Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Shioya
S
S
Katoh
H
H
Tsuji
C
C
Tsuda
M
M
Ohta
Y
Y
D000704:Analysis of Variance; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D015870:Gene Expression / Q000528:radiation effects; D006098:Granulocytes / Q000528:radiation effects; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000528:radiation effects*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis*; D008214:Lymphocytes / Q000528:radiation effects; D016676:Macrophages, Alveolar / Q000276:immunology / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D011833:Radiation Injuries, Experimental / Q000276:immunology* / Q000473:pathology; D051381:Rats; D013997:Time Factors
D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1