DNA adducts in tissues of germ-free and conventional rats fed high- and low-esterified pectins.
Tissue samples from germ-free and conventional rats fed pectins with different degrees of esterification (DE) were tested for containing indigenous DNA adducts (I-compounds). For 21 days groups of six germ-free and 10 conventional rats each were fed either pectin-free or with diets containing 7.5% of three differently esterified pectin preparations (Pectin A, DE 92.6%; Pectin B, DE 70.8%; Pectin C, DE 34.5%). DNA was isolated from colonic mucosa, liver, lung, kidney and analysed for DNA adducts by the 32P-postlabelling assay. The experiments showed a tendency to a higher number and intensity of DNA-adduct-like spots (1) in the colonic mucosa compared with liver, lung, and kidney, (2) in germ-free compared with conventional rats, (3) with pectin-free compared with pectin-containing diets, (4) under the low-esterified compared with the higher-esterified pectins.
Zunft
H J
HJ
Department of Epidemiology and Nutritional Behaviour, German Institute of Human Nutrition, Bergholz-Rehbruecke. zunft@ern.dife.d400.de
Goldin-Lang
P
P
Dongowski
G
G
D000818:Animals; D018736:DNA Adducts / Q000032:analysis*; D004951:Esterification; D005856:Germ-Free Life / Q000502:physiology; D007413:Intestinal Mucosa / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry*; D008168:Lung / Q000737:chemistry; D010368:Pectins / Q000494:pharmacology*; D051381:Rats
D018736:DNA Adducts; D010368:Pectins