title: The spectrum of allergy to South African bony fish (Teleosti). Evaluation by double-blind, placebo-controlled challenge.
abstract: OBJECTIVE
  The aim of this study was to assess the spectrum of allergy to South African bony fish (Class Teleosti), crustaceans and molluscs and to confirm or refute suspected allergy, specifically to bony fish, by double-blind, placebo-controlled food challenge (DBPCFC).
  
  
  METHODS
  Patients were recruited by means of a seafood allergy questionnaire. Subjects with reported allergy to hake, yellowtail, salmon and mackerel were investigated by means of skin-prick tests, RASTs and Western blot analysis. For those subjects with test results that were either all negative or equivocal, a definitive diagnosis of clinical sensitivity was made on the basis of DBPCFC.
  
  
  METHODS
  Volunteer population-based cohort in the Western Cape.
  
  
  METHODS
  105 volunteer subjects with suspected fish allergy were recruited by advertising in the local press.
  
  
  RESULTS
  Species-specific bony fish allergy was confirmed or refuted by DBPCFC.
  
  
  RESULTS
  The four most common seafood species reported to cause adverse reactions were prawns (46.7%), crayfish (43.8%), abalone (35.2%) and black mussels (33.3%). The four most common bony fish species to cause reactions were hake (24.8%), yellowtail (21.9%), salmon (15.2%) and mackerel (15.2%). Seven DBPCFCs were performed and two open challenges. Skin-prick tests produced one false-negative result. Western blots produced one false-negative and one false-positive result. The RAST had a 100% correlation with DBPCFC.
  
  
  CONCLUSIONS
  Local bony fish represent a significant cause of clinical reactions to seafood in the Western Cape. Although skin-prick tests, RASTs and Western blotting tests assist in the documentation of an IgE responder state, confirmation of clinical sensitivity can only be made with certainty by means of DBPCFC.
authors.affiliation: Department of Nutrition and Dietetics, Groote Schuur Hospital, Cape Town.
authors.forename: C
authors.initials: C
authors.lastname: Zinn
authors.forename: A
authors.initials: A
authors.lastname: Lopata
authors.forename: M
authors.initials: M
authors.lastname: Visser
authors.forename: P C
authors.initials: PC
authors.lastname: Potter
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015153:Blotting, Western; D002648:Child; D003445:Crustacea / Q000276:immunology; D004311:Double-Blind Method; D005399:Fishes / Q000276:immunology; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000209:etiology* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008875:Middle Aged; D008974:Mollusca / Q000276:immunology; D011852:Radioallergosorbent Test; D017747:Seafood / Q000009:adverse effects*; D012882:Skin Tests; D013019:South Africa; D013997:Time Factors
chemical_list: D007073:Immunoglobulin E