Differential regulation of HIV-1 fusion cofactor expression by CD28 costimulation of CD4+ T cells.
Activation of CD4(+) T lymphocytes from human immunodeficiency virus-type 1 (HIV-1)-infected donors with immobilized antibodies to CD3 and CD28 induces a virus-resistant state. This effect is specific for macrophage-tropic HIV-1. Transcripts encoding CXCR4/Fusin, the fusion cofactor used by T cell line-tropic isolates, were abundant in CD3/CD28-stimulated cells, but transcripts encoding CCR5, the fusion cofactor used by macrophage-tropic viruses, were not detectable. Thus, CD3/CD28 costimulation induces an HIV-1-resistant phenotype similar to that seen in some highly exposed and HIV-uninfected individuals.
Carroll
R G
RG
Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, MD 20850, USA.
Riley
J L
JL
Levine
B L
BL
Feng
Y
Y
Kaushal
S
S
Ritchey
D W
DW
Bernstein
W
W
Weislow
O S
OS
Brown
C R
CR
Berger
E A
EA
June
C H
CH
St Louis
D C
DC
D000911:Antibodies, Monoclonal / Q000276:immunology; D018106:CD28 Antigens / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000235:genetics*; D016853:Muromonab-CD3 / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D018121:Receptors, Cytokine / Q000235:genetics; D015728:Receptors, HIV / Q000235:genetics*; D015854:Up-Regulation; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D018106:CD28 Antigens; D017252:CD3 Complex; D007376:Interleukin-2; D008565:Membrane Proteins; D016853:Muromonab-CD3; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D018121:Receptors, Cytokine; D015728:Receptors, HIV