Soluble factor of transformed B lymphocytes in patients with HIV infection stimulates monocytes to produce PGE2.
Prostaglandins of the E series (PGE) were measured in the culture supernatants of peripheral blood mono-nuclear cells (PBMC) obtained from patients with human immunodeficiency virus (HIV) infection. Cell culture supernatants from 6 patients with acquired immunodeficiency disease (AIDS) and 11 patients with AIDS-related complex contained significantly higher concentrations of PGE than those from a group of 6 healthy HIV-negative male homosexual control subjects. The PGE producer cells were among the population of plastic-adherent PBMC. Transformed B lymphocyte cell lines spontaneously derived from the blood of patients with AIDS secrete a factor that stimulates normal donor adherent PBMC to produce PGE. The HIV permissive H9 cell line stimulated a significantly lower level of PGE production, which was not associated with HIV infection.
Tarter
T H
TH
Department of Urology, University of Southern California School of Medicine, Los Angeles 90089, USA.
Cunningham-Rundles
S
S
Laurence
J
J
Koide
S S
SS
D000386:AIDS-Related Complex / Q000473:pathology; D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology; D001402:B-Lymphocytes / Q000276:immunology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology*; D015658:HIV Infections / Q000276:immunology* / Q000473:pathology*; D015497:HIV-1 / Q000737:chemistry*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D008213:Lymphocyte Activation; D016676:Macrophages, Alveolar / Q000378:metabolism; D008297:Male; D013997:Time Factors; D014407:Tumor Cells, Cultured
D015699:HIV Envelope Protein gp120; D015232:Dinoprostone