PCR detection of Listeria monocytogenes in 'gravad' rainbow trout.
'Gravad' rainbow trout artificially contaminated with Listeria monocytogenes was analyzed by use of a 4 h enrichment period followed by extraction of DNA and PCR amplification. This procedure made it possible to detect 10-100 cfu L. monocytogenes per gram 'gravad' rainbow trout, within 12 h. After a prolonged enrichment period of 24 h, numbers as low as 1-10 cfu L. monocytogenes per gram could be detected. The method described may be a useful tool for screening samples of 'gravad' rainbow trout for the presence of L. monocytogenes, since it is sensitive, rapid and simple.
Ericsson
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Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden. Henrik.Ericsson@lmhyg.slu.se
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D000818:Animals; D001483:Base Sequence; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005511:Food Handling / Q000592:standards; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000209:etiology; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013095:Spermidine / Q000032:analysis; D013548:Sweden; D013997:Time Factors
D004269:DNA, Bacterial; D013095:Spermidine