Influence of different rol gene products on the chain length of Shigella dysenteriae type 1 lipopolysaccharide O antigen expressed by Shigella flexneri carrier strains.
Introduction of the rol genes of Shigella dysenteriae 1 and Escherichia coli K-12 into Shigella flexneri carrier strains expressing the heterologous S. dysenteriae type 1 lipopolysaccharide resulted in the formation of longer chains of S. dysenteriae 1 O antigen. In bacteria producing both homologous and heterologous O antigen, this resulted in a reduction of the masking of heterologous O antigen by homologous lipopolysaccharide and an increased immune response induced by intraperitoneal immunization of mice by recombinant bacteria. The rol genes of S. dysenteriae 1 and E. coli K-12 were sequenced, and their gene products were compared with the S. flexneri Rol protein. The primary sequence of S. flexneri Rol differs from both E. coli K-12 and S. dysenteriae 1 Rol proteins only at positions 267 and 270, which suggests that this region may be responsible for the difference in biological activities.
Klee
S R
SR
Division of Microbiology, GBF-National Research Centre for Biotechnology, Braunschweig, Germany.
Tzschaschel
B D
BD
Timmis
K N
KN
Guzman
C A
CA
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001428:Bacterial Vaccines / Q000235:genetics*; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D051379:Mice; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012762:Shigella dysenteriae / Q000235:genetics* / Q000276:immunology; D012763:Shigella flexneri; D014614:Vaccines, Synthetic / Q000235:genetics
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D029968:Escherichia coli Proteins; D019081:O Antigens; D014614:Vaccines, Synthetic; C489586:wzzB protein, E coli; C075969:rol protein, Bacteria