title: CD45RA-RO+ (memory) but not CD45RA+RO- (naive) T cells roll efficiently on E- and P-selectin and vascular cell adhesion molecule-1 under flow.
abstract: This study examines the molecular mechanisms that underlie the observed preferential interactions of memory vs naive T cells with activated vascular endothelium. Many more CD4+ CD45RO+ (memory) cells adhered to 6-h TNF-alpha-activated human umbilical vein endothelium under flow than CD4+CD45RA+ (naive) cells. Adhesion studies were performed using Chinese hamster ovary (CHO) cell monolayers expressing human E- or P-selectin (CHO-E and CHO-P, respectively) or with soluble vascular cell adhesion molecule-1 (VCAM-1)-coated glass surfaces. Under flow at 1.8 dynes/cm2, RO+ T cells rolled extensively at low velocity on both CHO-P and CHO-E monolayers and VCAM-1, whereas very few RA+ T cells interacted with these surfaces. VCAM-1-dependent rolling was blocked completely by anti-very late Ag-4 (VLA-4) Abs. Purified CD4+RA+ T cells could be converted to RO+ cells by mitogen stimulation and 7-day culture in vitro, and this correlated with the acquisition of the ability to roll on E- or P-selectin, but not on VCAM-1 under flow. In summary, these data indicate that CD45RO+ cells interact with E- and P-selectins and VCAM-1 much more effectively than do CD45RA+ cells under flow conditions, and these adhesion pathways may contribute, either individually or in combination, to the preferential recruitment of memory T cells to peripheral sites of inflammation.
authors.affiliation: Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02160, USA.
authors.forename: A H
authors.initials: AH
authors.lastname: Lichtman
authors.forename: H
authors.initials: H
authors.lastname: Ding
authors.forename: L
authors.initials: L
authors.lastname: Henault
authors.forename: G
authors.initials: G
authors.lastname: Vachino
authors.forename: R
authors.initials: R
authors.lastname: Camphausen
authors.forename: D
authors.initials: D
authors.lastname: Cumming
authors.forename: F W
authors.initials: FW
authors.lastname: Luscinskas
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000276:immunology; D006224:Cricetinae; D019040:E-Selectin / Q000276:immunology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory; D017493:Leukocyte Common Antigens; D019007:P-Selectin / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000276:immunology*
chemical_list: D019040:E-Selectin; D019007:P-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D017493:Leukocyte Common Antigens