In vivo antigenotoxic effects of dietary allyl sulfides in the rat.
The effects of dietary administration of diallyl sulfide (DAS), diallyl disulfide (DADS) and allyl mercaptan (AM) on the genotoxicity of different chemicals were studied in two experimental systems: (i) measurement of hepatic DNA single-strand breaks induced in rats by aflatoxin B1 (AFB1), N-nitrosodimethylamine (NDMA) or methylnitrosourea (MNU); (ii) mutagenicity of AFB1 or NDMA on Salmonella typhimurium TA100 using hepatic S9 from rats fed allyl sulfides as the activation system. All compounds strongly reduced hepatic DNA breaks induced by AFB1 and NDMA but did not modify the genotoxicity of MNU. In the Ames test, the mutagenicity of NDMA was strongly inhibited by hepatic S9 from rats fed either compound. The mutagenicity of AFB1 was also reduced but to a lesser extent. Such effects are likely related to the modulation of drug-metabolizing enzymes which play a key role in metabolic activation as well as detoxication of NDMA and AFB1.
Le Bon
A M
AM
Unité de Toxicologie Nutritionnelle, INRA, Dijon, France.
Roy
C
C
Dupont
C
C
Suschetet
M
M
D016604:Aflatoxin B1 / Q000494:pharmacology; D000498:Allyl Compounds; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D016587:Antimutagenic Agents / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D004128:Dimethylnitrosamine / Q000494:pharmacology; D004220:Disulfides / Q000494:pharmacology; D008099:Liver / Q000187:drug effects; D008297:Male; D008770:Methylnitrosourea / Q000494:pharmacology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology; D051381:Rats; D012486:Salmonella typhimurium / Q000502:physiology; D013440:Sulfides / Q000494:pharmacology*
D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D016587:Antimutagenic Agents; D004220:Disulfides; D009153:Mutagens; D013440:Sulfides; C028009:diallyl disulfide; C038491:allyl sulfide; D008770:Methylnitrosourea; D016604:Aflatoxin B1; D004128:Dimethylnitrosamine