Virulence patterns from septicemic Escherichia coli O78 strains.
Several septicemic Escherichia coli O78 strains, isolated from different sources, were characterized phenotypically and genotypically. Two avian isolates, one of which is known to carry the AC/I fimbriae, hybridized with the sfa determinant in colony dot-blot assay. Southern hybridizations with specific sfa probes, following pulsed-field gel electrophoresis (PFGE), showed positive hybridization to the same fragment in each of these strains. Determination of the N-terminal amino acid sequence of the AC/I major subunit gene revealed high similarity to the sequence of the SfaA-II protein. These data suggest that the adhesin gene cluster, coding for AC/I fimbriae, belongs to the S-fimbrial adhesin family.
Babai
R
R
Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.
Blum-Oehler
G
G
Stern
B E
BE
Hacker
J
J
Ron
E Z
EZ
D018829:Adhesins, Bacterial / Q000032:analysis / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D016667:Bacterial Capsules / Q000737:chemistry / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D015139:Blotting, Southern; D002645:Chickens; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D006877:Hydroxamic Acids / Q000032:analysis / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D015183:Restriction Mapping; D018805:Sepsis / Q000382:microbiology*; D012703:Serotyping; D012756:Sheep; D017262:Siderophores / Q000032:analysis / Q000235:genetics; D014422:Turkeys; D014774:Virulence
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D006877:Hydroxamic Acids; D017262:Siderophores; C031819:aerobactin