[Hyaluronic acid (hyaluronan) levels in pathological human saphenous veins. Effects of procyanidol oligomers].
We investigated the hyaluronan content in the pathologic human venous wall using an ELSA assay with hyaluronectin according to the method of Delpech et al. The mean hyaluronan content in the 74 fragments from 12 venous walls studied was 596 +/- 528 ng/mg dry weight. These 12 venous walls could be separated in 3 distinct groups according to their hyaluronan content, low (277 +/- 141 ng/mg dry weight), moderate (552 +/- 361 ng/m dry weight) or high (1299 +/- 568 ng/mg dry weight). The differences between these groups are significant (p < 0.001). The presence of a veino-lymphatic oedema was generally associated with a high hyaluronan level (in 65% of cases). The 3H-glucosamine incorporation in cultured venous wall explants showed a 35% increase (p < 0.002) in varicosis as compared with the non or less modified segments of the vein and a 29% (p < 0.001) increase in presence of a veino-lymphatic oedema. The addition of 1 mg/ml of PCO (Procyanidolic Oligomers) to the culture media induced near to 20% decrease of the 3H-glucosamine incorporation and a 34% decrease of the hyaluronan content. Our results confirm the role of local overproduction of hyaluronan in the establishment of oedema and the potential effect of PCO to counteract it.
Drubaix
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Laboratoire de Biologie Cellulaire, Université Paris VII, France.
Maraval
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Robert
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Robert
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D000367:Age Factors; D000959:Antihypertensive Agents / Q000494:pharmacology*; D044946:Biflavonoids; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004487:Edema / Q000097:blood; D005260:Female; D005944:Glucosamine / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000097:blood*; D008875:Middle Aged; D044945:Proanthocyanidins; D012501:Saphenous Vein / Q000737:chemistry*; D014648:Varicose Veins / Q000097:blood
D000959:Antihypertensive Agents; D044946:Biflavonoids; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin; D006820:Hyaluronic Acid; D005944:Glucosamine
Taux d'acide hyaluronique (hyaluronane) dans les veines saphènes humaines pathologiques. Effet des oligomères procyanidoliques.