Development of a urodilatin-specific antibody and radioimmunoassay for urodilatin in human urine.
Urodilatin (URO) (95-126) is a renal-derived natriuretic peptide that is isolated only from human urine. This study describes the development of a URO-specific antibody and a RIA for URO in urine. At present, there is no commonly available URO-specific antibody. We produced a URO-specific antibody without cross-reactivity with atrial natriuretic peptide (ANP) analogs by immunization of rabbits with the URO (95-126) peptide and subsequent purification of the resulting URO antiserum with affinity chromatography with CNBr-activated Sepharose 4B. The urine samples were ethanol-extracted before assay. The CVs were 6.7% (intraassay) and 14.1% (interassay). This study reports the circadian urinary excretion of URO in 24 healthy subjects with seven sampling periods per 24 h.
Carstens
J
J
Research Laboratory of Nephrology and Hypertension, Aarhus University Hospital, Skejby Hospital, Denmark.
Jensen
K T
KT
Ivarsen
P
P
Rasmussen
L M
LM
Pedersen
E B
EB
D000328:Adult; D000368:Aged; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D000918:Antibody Specificity; D009320:Atrial Natriuretic Factor / Q000276:immunology / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D002940:Circadian Rhythm; D004355:Drug Stability; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology / Q000652:urine*; D011863:Radioimmunoassay / Q000379:methods*; D012016:Reference Values; D012680:Sensitivity and Specificity
D000906:Antibodies; D007457:Iodine Radioisotopes; D010446:Peptide Fragments; C056452:Ularitide; D009320:Atrial Natriuretic Factor