title: Late postnatal mother-to-child transmission of HIV-1 in Abidjan, Côte d'Ivoire.
abstract: BACKGROUND
  HIV-1 can be transmitted from an infected mother to her infant through breastfeeding, although the precise risk of transmission by this route is unknown. A long-term follow-up of children born to HIV-infected women in Abidjan, Côte d'Ivoire, has enabled us to estimate this risk.
  
  
  METHODS
  Children born to 138 HIV-1-seropositive women, 132 HIV-2-seropositive women, 69 women seroreactive to both HIV-1 and HIV-2, and 274 HIV-seronegative women were enrolled at birth and followed up for as long as 48 months. All children were breastfed (median duration 20 months). Blood samples for either or both HIV PCR and HIV serology were obtained at 1, 2, and 3 months of age, and every 3 months thereafter. Early HIV infection was defined as a positive HIV-1 PCR result obtained in the first 6 months of life. Late postnatal transmission was diagnosed when a child had a negative PCR at 3 or 6 months of age, followed by either or both a positive HIV-1 PCR at 9 months or older, or persistently positive HIV-1 serology at 15 months or older.
  
  
  RESULTS
  82 children born to HIV-1-seropositive mothers and 57 children born to mothers seropositive for both HIV-1 and HIV-2 had PCR results for samples taken within the first 6 months. By 6 months of age, 23 (28%; 95% CI 19-39) of the 82 children born to HIV-1-seropositive mothers and ten (18%; 95% CI 9-30) of the 57 children born to dually seropositive mothers were HIV-1 infected. Among children whose PCR results were negative at or before age 6 months, and who were followed up beyond 6 months, an additional four (9%) of the 45 children born to HIV-1-seropositive mothers and two (5%) of the 39 children born to dually seropositive mothers became HIV infected. The estimated rates of late postnatal transmission, with account taken of loss to follow-up and the observed pattern of weaning, were 12% (95% CI 3-23) and 6% (0-14), respectively. One of the five children whose mothers seroconverted from HIV-negative to HIV-1, and one of seven children whose mothers seroconverted from HIV-2 to dual reactivity, became HIV-1 positive. No case of late postnatal transmission occurred in children born to HIV-2-positive or persistently HIV-negative mothers.
  
  
  CONCLUSIONS
  Breastfed children born to mothers seropositive for HIV-1 alone or seropositive for HIV-1 and HIV-2 in Abidjan are at substantial risk of late postnatal transmission. Early cessation of breastfeeding at 6 months of age should be assessed as a possible intervention to reduce postnatal transmission of HIV.
authors.affiliation: Project RETRO-CI, Abidjan, Côte d'Ivoire.
authors.forename: E R
authors.initials: ER
authors.lastname: Ekpini
authors.forename: S Z
authors.initials: SZ
authors.lastname: Wiktor
authors.forename: G A
authors.initials: GA
authors.lastname: Satten
authors.forename: G T
authors.initials: GT
authors.lastname: Adjorlolo-Johnson
authors.forename: T S
authors.initials: TS
authors.lastname: Sibailly
authors.forename: C Y
authors.initials: CY
authors.lastname: Ou
authors.forename: J M
authors.initials: JM
authors.lastname: Karon
authors.forename: K
authors.initials: K
authors.lastname: Brattegaard
authors.forename: J P
authors.initials: JP
authors.lastname: Whitaker
authors.forename: E
authors.initials: E
authors.lastname: Gnaore
authors.forename: K M
authors.initials: KM
authors.lastname: De Cock
authors.forename: A E
authors.initials: AE
authors.lastname: Greenberg
mesh_terms: D001942:Breast Feeding / Q000009:adverse effects*; D007560:Cote d'Ivoire; D005260:Female; D015658:HIV Infections / Q000635:transmission* / Q000821:virology; D006679:HIV Seropositivity / Q000821:virology; D015497:HIV-1; D015498:HIV-2; D006801:Humans; D007223:Infant; D018445:Infectious Disease Transmission, Vertical; D012307:Risk Factors; D013997:Time Factors