Immunocompetent cells in rat periodontal ligament and their recruitment incident to experimental orthodontic tooth movement.
The aims of this study were to evaluate the number and distribution of immunocompetent cells in normal rat periodontal ligament (PDL) and to quantify their recruitment incident to experimental tooth movement. 27 young animals had the 1st right maxillary molar moved mesially by an orthodontic appliance for 1, 3, 7 and 14 days, respectively. 4 animals served as untreated controls. An immunohistochemical procedure was carried out on alternate serial cryostat sections, and monoclonal antibodies against CD11b (macrophages, dendritic cells), CD43 (lymphocytes, polymorphs), CD4 (helper T-lymphocytes), and class II MHC molecules were used. Mean counts of the immunolabeled cells in the control group showed the highest number of CD11b+ and class II molecule expressing cells, while CD4+ and CD43+ cells were scarcely found. Significant increase in the number of CD11b+, CD43+ cells and class II molecules was found in the PDL of the experimentally moved 1st molars compared with the contralateral side and the control group, while CD4+ cells showed no significant increase. CD11b+ and cells expressing class II molecules were found around hyalinized tissue, between dentin and cellular cementum and close to Malassez' epithelial cells. In conclusion, normal rat PDL has high number of macrophage and dendritic-like cells, but few lymphocytes and granulocytes. Furthermore, experimental tooth movement leads to significant recruitment of cells belonging to the mononuclear phagocytic system, but has no significant effect on the number of lymphocytes and granulocytes in the rat PDL.
Vandevska-Radunovic
V
V
Department of Anatomy and Cell Biology, University of Bergen, Norway. Vaska.Vandevska-Radunovic@pki.uib.no
Kvinnsland
I H
IH
Kvinnsland
S
S
Jonsson
R
R
D000818:Animals; D015703:Antigens, CD; D018845:CD11 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D003713:Dendritic Cells / Q000166:cytology; D003739:Dental Cementum / Q000166:cytology; D003804:Dentin / Q000166:cytology; D004847:Epithelial Cells; D006098:Granulocytes / Q000166:cytology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006818:Hyalin; D007150:Immunohistochemistry; D051917:Leukosialin; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000166:cytology; D008264:Macrophages / Q000166:cytology; D008297:Male; D008437:Maxilla; D008963:Molar; D009504:Neutrophils / Q000166:cytology; D010513:Periodontal Ligament / Q000166:cytology*; D010586:Phagocytes / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000032:analysis; D013601:T-Lymphocytes / Q000166:cytology*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology; D014087:Tooth Movement Techniques
D015703:Antigens, CD; D018845:CD11 Antigens; D000949:Histocompatibility Antigens Class II; D051917:Leukosialin; D012795:Sialoglycoproteins; C497864:Spn protein, rat