title: Rainbow Universal CPG: a versatile solid support for oligonucleotide synthesis.
abstract: We have developed a universal solid support, termed Rainbow Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore glass (CPG) support. Deprotection of oligodeoxyribonucleotides was optimized using 0.5 M LiCl in concentrated ammonium hydroxide. PCR experiments using three different sets of primers proved that the 3'-hydroxyl function of oligodeoxyribonucleotides synthesized from Rainbow Universal CPG was retained. This universal solid support shows promise for replacing the standard nucleoside CPG supports.
authors.affiliation: CLONTECH Laboratories, Inc., Palo Alto, CA, USA.
authors.forename: P S
authors.initials: PS
authors.lastname: Nelson
authors.forename: S
authors.initials: S
authors.lastname: Muthini
authors.forename: M
authors.initials: M
authors.lastname: Vierra
authors.forename: L
authors.initials: L
authors.lastname: Acosta
authors.forename: T H
authors.initials: TH
authors.lastname: Smith
mesh_terms: D001331:Automation; D001710:Biotin; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D005898:Glass; D006801:Humans; D018021:Lithium Chloride; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D010920:Placenta / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013696:Temperature; D013698:Templates, Genetic
chemical_list: D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D001710:Biotin; D018021:Lithium Chloride