title: cDNA cloning of eel (Anguilla anguilla) somatolactin.
abstract: The nucleotide sequence of the cDNA coding for eel somatolactin (SL), a pituitary hormone belonging to the growth hormone (GH)/prolactin (PRL) family, has been determined. The full-length eel SL cDNA (1213 bp) encodes a protein of 229 amino acids (aa), with a putative signal peptide of 24 aa and a mature protein of 204 aa. Eel SL contains seven Cys residues found to be characteristic of SLs, and two potential N-glycosylation sites. Significant sequence homology between eel and seven other fish SLs (42% aa identity, 63% aa similarity) reveal SL to be highly conserved. A higher sequence identity of SL to GH than PRL is suggested by the comparison of these hormones in eel and chum salmon.
authors.affiliation: Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex, UK.
authors.forename: D
authors.initials: D
authors.lastname: May
authors.forename: C M
authors.initials: CM
authors.lastname: Todd
authors.forename: M
authors.initials: M
authors.lastname: Rand-Weaver
mesh_terms: D000595:Amino Acid Sequence; D000811:Anguilla / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D029941:Fish Proteins; D005399:Fishes / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics; D008969:Molecular Sequence Data; D010907:Pituitary Hormones / Q000235:genetics*; D011388:Prolactin / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; C064448:somatolactin protein, fish; D011388:Prolactin; D013006:Growth Hormone