ATP modification of serotonin-induced contraction of the rat pulmonary artery.
Serotonin (5HT) and ATP are simultaneously released from activated platelets at the site of vascular injury and are hypothesized to play a significant role in hemostasis. Our laboratory investigated the modulation of vascular contraction of arterial ring segments by 5HT plus ATP as a model of the potential regulation of localized vascular tone by platelet releasates in regions of arterial damage. This study expands our focus on how these two vasoactive components, released from platelet dense granules, regulate vascular tone. 5HT- and 5HT analog-induced vasoconstrictions were measured in the presence or absence of ATP and ATP analogs with intact or deendothelialized rat pulmonary arterial ring segments suspended in organ baths. The possible presence of 5HT2 and 5HT1A receptor types in the rat pulmonary artery was demonstrated by vasoconstrictions induced by 5HT and (+)-8-hydroxy-2-(di-N-propylamino) tetralin hydrobromide (DPAT). The DPAT response was only 30%-50% of that induced by comparable concentrations of 5HT. The 5HT-induced contraction was inhibited by the 5HT2 antagonist, ketanserin. ATP equally relaxed 5HT and DPAT contracted tissue while the P2X agonist, alphabeta-methylene ATP, increased the contracted state of DPAT-treated arteries to a significantly greater extent than observed with 5HT. The P2y agonist, 3'-O-(4-benzoyl)benzoyl ATP (BzATP), the P2X agonist betagamma-methylene ATP, and ATP all relaxed 5HT-induced contractions to similar levels under a number of physiological conditions. The final level of 5HT-induced tissue contraction was the same whether ATP was added prior to, after, or simultaneously with 5HT. ATP and the phosphodiesterase inhibitor, theophylline, inhibited 5HT-induced vasoconstriction in an additive fashion. The ATP effects were endothelium dependent, while the inhibition by theophylline was not. The distribution of 5HT and ATP receptor types, as indicated by these and numerous other studies, appears to vary within different regions of the cardiovascular system. Extracellular ATP can synergistically enhance or inhibit 5HT-contracted blood vessels differentially at localized regions, which would significantly impact on localized vascular tone, and this in turn may modulate hemostasis and thrombosis.
Soslau
G
G
Department of Biochemistry, MCP-Hahnemann School of Medicine, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19102-1192, USA.
Silverberg
M
M
Brodsky
I
I
McCarty
P P
PP
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D000242:Cyclic AMP / Q000502:physiology; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011651:Pulmonary Artery; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology; D014661:Vasoconstriction / Q000502:physiology
D010726:Phosphodiesterase Inhibitors; D012702:Serotonin Antagonists; D017366:Serotonin Receptor Agonists; D012701:Serotonin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000255:Adenosine Triphosphate; D013806:Theophylline; D000242:Cyclic AMP