title: House-dust mite allergen Der p 1: amount or concentration?
abstract: There is a lack of consensus as to the best way to quantify settled house-dust mite Der p 1 allergen from planar and nonplanar surfaces. To investigate the most appropriate measure of settled house-dust mite Der p 1 allergen, we determined the relationship between Der p 1 allergen content and collected dust weight from planar-surface (mattress) and nonplanar-surface (living-room) samples in 58 homes. There was a direct relationship between Der p 1 content and dust weight for both planar-surface (mattress, n = 217) samples (r = 0.50, P < 0.0001) and nonplanar-surface (living-room, n = 48) samples (r = 0.61, P < 0.0001). Correction for the surface area of the planar surface (mattress) vacuum-cleaned resulted in no additional benefit. We conclude that expression of Der p 1 levels per weight of collected dust appears to be the best method for both planar and nonplanar surfaces.
authors.affiliation: University Medicine, Southampton General Hospital, UK.
authors.forename: I J
authors.initials: IJ
authors.lastname: Doull
authors.forename: J
authors.initials: J
authors.lastname: Bright
authors.forename: P
authors.initials: P
authors.lastname: Yogeswaran
authors.forename: P H
authors.initials: PH
authors.lastname: Howarth
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D039741:Antigens, Dermatophagoides; D001513:Beds; D004306:Dose-Response Relationship, Immunologic; D004391:Dust / Q000032:analysis*; D005430:Floors and Floorcoverings; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008925:Mites / Q000276:immunology*
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D004391:Dust; D006023:Glycoproteins