Cloning and functional expression of the rat brain KIR6.2 channel.
We obtained cDNA encoding KIR6.2 channel from the rat brain cDNA library. The reconstituted K+ channel with KIR6.2 and SUR1 genes retained properties similar to those reported in the native ATP-sensitive K+ channel including run-down and recovery of channel activity. When KIR6.2 was co-expressed with CFTR or MDR1, no K+ channel activity was recorded.
Takano
M
M
Department of Physiology and Biophysics, Faculty of Medicine, Kyoto University, Japan. takano@med.kyoto.u.ac.jp
Ishii
T
T
Xie
L H
LH
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D019556:COS Cells; D003001:Cloning, Molecular; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism; D024661:Potassium Channels, Inwardly Rectifying; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D018076:DNA, Complementary; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D011994:Recombinant Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator