Evaluation of an aroA mutant Salmonella typhimurium vaccine in chickens using modified semisolid Rappaport Vassiliadis medium to monitor faecal shedding.
In groups of chickens vaccinated orally or intramuscularly with a live aroA mutant Salmonella typhimurium vaccine strain and then experimentally inoculated with 10(8) CFU of wild type S. typhimurium or 10(9) CFU of S. enteritidis, faecal shedding of the vaccine and wild type strains was monitored by the buffered peptone water-modified semisolid Rappaport Vassiliadis medium method, which detected less than 10(2) CFU per gram of faeces. The vaccine strain was shed in the faeces for up to 26 days. Vaccination failed to reduce the faecal shedding of wild type S. typhimurium or S. enteritidis. The variation in the shedding patterns of chickens within each group was greater than between treatment groups.
Tan
S
S
Department of Veterinary Microbiology and Immunology, University of Guelph, Ont., Canada. tans@em.agr.ca
Gyles
C L
CL
Wilkie
B N
BN
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases; D000818:Animals; D001428:Bacterial Vaccines; D002645:Chickens; D003470:Culture Media; D005243:Feces / Q000382:microbiology; D011201:Poultry Diseases; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000517:prevention & control; D012477:Salmonella enteritidis / Q000276:immunology / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014166:Transferases / Q000235:genetics* / Q000276:immunology
D001428:Bacterial Vaccines; D003470:Culture Media; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase