A new procedure for establishing functional monoclonal antibodies capable of inhibiting E- or P-selectin-dependent cell adhesion.
Employing a new procedure, we established many monoclonal antibodies (mAbs) which inhibit E- or P-selectin-dependent cell adhesion. One of these mAbs is capable of staining selectin in paraffin-embedded histological sections. The procedure is based on immunization of BALB/c mice with irradiated mouse myeloma NS-1 cells (syngeneic HAT-sensitive fusion partner cells) transfected with cDNA encoding human E- or P-selectin. Resulting NS-1 transfectant cells permanently express human E- or P-selectin as immunogen. The mAbs are useful for detecting selectins by flow cytometric and immunohistological methods, and for inhibiting selectin-dependent adhesion in experimental models. In contrast, the majority of anti-selectin mAbs previously established do not have these capabilities.
Handa
K
K
Biomembrane Institute, Seattle, WA 98119, USA.
White
T
T
Hakomori
S I
SI
Hirohashi
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D004727:Endothelium / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018922:HL-60 Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology / Q000473:pathology; D019007:P-Selectin / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D016612:Paraffin Embedding; D013194:Staining and Labeling / Q000379:methods; D014162:Transfection; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D019040:E-Selectin; D019007:P-Selectin