Effect of c-Fos overexpression on the murine macrophage cell line P388DI.
In order to study the role of Fos on the regulation of proliferation in the monocyte-macrophage lineage we realized a stable transfection of the murine P388D1 cell line by the murine c-fos gene under the control of the human metallothionein IIA promoter. Several clones have been selected by geneticin: they show a variable number of integrated transgene (two to ten copies). Their expression has been analyzed in the presence or absence of cadmium chloride as inducer (5 x 10(-6) M). In one clone especially, the c-fos mRNA and Fos protein levels were respectively 6- and 10-fold increased. The study of cell growth by tritiated thymidine incorporation indicates a negative effect of the overexpressed Fos protein in the absence of any other stimulus.
Mirahmadi
R
R
Laboratoire d'histologie, embryologie, cytogénétique, faculté de médecine Cochin-Port-Royal, Paris, France.
Le Garrec
Y
Y
Rouer
E
E
Michel
P
P
Pompidou
A
A
D000818:Animals; D019256:Cadmium Chloride / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos / Q000235:genetics*; D007941:Leukemia P388 / Q000503:physiopathology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000235:genetics / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D019256:Cadmium Chloride