Effect of intraperitoneal and intrahippocampal (CA1) 2-chloroadenosine in amygdaloid kindled rats.
Effects of intraperitoneal and intrahippocampal 2-chloroadenosine and caffeine were examined in fully kindled amygdaloid rats. Intraperitoneal administration of 2-chloroadenosine (5 and 10 mg/kg) decreased afterdischarge duration, stage 5 seizure duration and prolonged time taken to reach stage 4 seizure. Only the 10 mg/kg dose induced a significant reduction in seizure stage. Intraperitoneal administration of caffeine (50 mg/kg) increased both afterdischarge duration and stage 5 seizure duration but did not significantly alter other parameters. Intrahippocampal microinfusion of 2-chloroadenosine (1 mM) or caffeine (2 mM) did not alter any of the measured seizure parameters. Intraperitoneal but not intrahippocampal pretreatment of animals with caffeine (50 mg/kg and 2 mM, respectively) blocked the anticonvulsant effects induced by intraperitoneal administration of 2-chloroadenosine. It may therefore be concluded that the adenosine A1 receptors of the CA1 region of the hippocampus do not play a role in mediating the anticonvulsant effects of intraperitoneally administered 2-chloroadenosine in amygdaloid kindled rats.
Pourgholami
M H
MH
Department of Pharmacology, Faculty of Medicine, Shaheed-Beheshti University of Medical Sciences, Tehran, Iran.
Mirnajafi-Zadeh
J
J
Behzadi
J
J
D015762:2-Chloroadenosine / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000679:Amygdala / Q000502:physiology*; D000818:Animals; D002110:Caffeine / Q000008:administration & dosage / Q000494:pharmacology; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D004594:Electrophysiology; D006624:Hippocampus; D007267:Injections; D007274:Injections, Intraperitoneal; D007696:Kindling, Neurologic; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000503:physiopathology
D000697:Central Nervous System Stimulants; D015762:2-Chloroadenosine; D002110:Caffeine