Active and alpha-1 proteinase inhibitor complexed leukocyte elastase levels in crevicular fluid from patients with periodontal diseases.
Human leukocyte elastase is present in large amounts in the crevicular fluid of patients with periodontal disease and was considered as a putative biological marker of the evolution of such diseases. The aim of this work was to measure spectrophotometrically amounts of active elastase (AE) and elastase complexed to alpha 1 proteinase inhibitor (E-alpha 1-PI) in gingival crevicular fluid obtained, from patients suffering from rapidly progressive periodontitis (RPP group) or adult periodontitis (AP group) with different probing depths (3 to 5 mm and > 6 mm). AE and E-alpha 1-PI concentrations were negligible in healthy individuals. AE, but not E-alpha 1-PI, concentration appears to vary significantly with the probing depth in patients suffering either from rapidly progressive or adult periodontitis. No correlations were found between levels of AE and E-alpha 1-PI in the different groups of patients. AE concentration seems to be a marker of periodontal diseases in relation with probing depth.
Meyer
J
J
Département de Pharmacologie, Hôpital Cochin, Paris, France.
Guessous
F
F
Huynh
C
C
Godeau
G
G
Hornebeck
W
W
Giroud
J P
JP
Roch-Arveiller
M
M
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D019272:Leukocyte Elastase / Q000032:analysis* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000201:enzymology / Q000473:pathology; D010518:Periodontitis / Q000201:enzymology* / Q000473:pathology; D015842:Serine Proteinase Inhibitors / Q000032:analysis* / Q000737:chemistry; D013053:Spectrophotometry; D000515:alpha 1-Antitrypsin / Q000032:analysis* / Q000737:chemistry
D015415:Biomarkers; D015842:Serine Proteinase Inhibitors; D000515:alpha 1-Antitrypsin; D019272:Leukocyte Elastase