A first catalog of genes involved in pig ovarian follicular differentiation.
As a first step toward the characterization of genetic expression in pig ovaries, we have selected 238 clones by differential hybridization from a pig granulosa cell cDNA library, using probes prepared from RNA extracted from either untreated or FSH-treated cells and, in order to generate expressed sequence tags (ESTs), we have performed 3' and 5' single-pass sequencing of these clones. Sequences of the 3' end of the 167 clones that produced informative sequence data were first compared with each other, revealing a redundancy level of 21%. Sequences from the 136 unique clones were analyzed for similarities with sequence data included in Genbank and EMBL databases. Among these unique clones, 54 (40%) matched significantly with sequences from either Genbank of EMBL: 4 with known genes in pig, 35 matched with previously reported human genes, and 15 with other mammalian genes. Eighty-two clones (60%) showed no significant match with any gene or DNA sequence in the Genbank and EMBL databases and thus may represent new pig transcripts.
Tosser-Klopp
G
G
Laboratoire de Génétique Cellulaire, Institut National de la Recherche Agronomique, Centre de Recherches de Toulouse, 31326 Castanet-Tolosan cedex, France.
Benne
F
F
Bonnet
A
A
Mulsant
P
P
Gasser
F
F
Hatey
F
F
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D016208:Databases, Factual; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology; D005786:Gene Expression Regulation; D015723:Gene Library; D006107:Granulosa Cells / Q000166:cytology*; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D013552:Swine
D018076:DNA, Complementary; D012333:RNA, Messenger; D005640:Follicle Stimulating Hormone