Increased expression of transforming growth factor-beta1 during gastric ulcer healing in rats.
This study was done to investigate the expression and localization of transforming growth factor-beta1 (TGF-beta1) in the gastric ulcerated tissues produced by acetic-acid during the healing process, by northern blot analysis and immunohistochemical technique. Ulcerated TGF-beta1 mRNA levels were significantly increased from days 3 to 18, in a similar manner to extracellular matrix proteins, and returned to control levels at the scarred phase. Immunoreactive TGF-beta1 was localized in epithelial cells beneath proliferative zone in intact tissues. In ulcerated tissues, TGF-beta1 was localized in macrophages in the ulcer bed and in fibroblasts or myofibroblasts in the granulation tissues. Treatment with prostaglandin E1 (PGE1) further stimulated ulcerated TGF-beta1 expression, being associated with the acceleration of gastric ulcer healing, while treatment with indomethacin reduced TGF-beta1 expression, being accompanied by the delayed ulcer healing. The combination of PGE1 and indomethacin reversed the indomethacin-induced decrease in ulcerated TGF-beta1. Thus, TGF-beta1 may be implicated in the acceleration of gastric ulcer healing.
Tominaga
K
K
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Arakawa
T
T
Kim
S
S
Iwao
H
H
Kobayashi
K
K
D019342:Acetic Acid; D000527:Alprostadil / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D015152:Blotting, Northern; D016326:Extracellular Matrix Proteins / Q000187:drug effects / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D007150:Immunohistochemistry; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000187:drug effects; D013276:Stomach Ulcer / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects / Q000235:genetics / Q000502:physiology*
D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D000527:Alprostadil; C045125:ornoprostil; D019342:Acetic Acid; D007213:Indomethacin