Differential APC requirements of self- and nonself-reactive T cells and T cell hybridomas specific for retinal S-antigen.
Autoreactive T cell hybridomas specific for pathogenic peptides of retinal S-Ag require a novel radiosensitive APC activity for IL-2 secretion that is distinct from Ag presentation by MHC class II. Antigen-dependent IL-2 secretion by self-reactive hybridomas was much more efficient with splenic APC than with thymic APC, although both provided similar levels of hybridoma TCR occupancy as measured by activation-induced cell death. Furthermore, thymic APC did stimulate IL-2 secretion by a non-self reactive hybridoma. To test the hypothesis that this activity was provided by a distinct cell population, fractionated splenocytes were tested for their ability to present Ag to these hybridomas. The most potent Ag presentation for IL-2 secretion was found to segregate with low-density, B cell-enriched fractions while adherent cells, or purified T cells were unable to support IL-2 production. Together with previous results, the data show that antigen presentation leading to IL-2 secretion by these autoreactive T cell hybridomas requires activated B cells, whereas TCR occupancy can be provided by several APC subsets.
Prasad
S A
SA
Department of Microbiology, University of Minnesota, Minneapolis 55455, USA.
Fling
S P
SP
Gregerson
D S
DS
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D019393:Arrestin / Q000276:immunology*; D001402:B-Lymphocytes; D002448:Cell Adhesion; D002454:Cell Differentiation; D002499:Centrifugation, Density Gradient; D005260:Female; D006825:Hybridomas; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats; D011917:Rats, Inbred Lew; D012160:Retina / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D019393:Arrestin; D007376:Interleukin-2