Effect of Brussels sprouts on oxidative DNA-damage in man.
The effect of consumption of Brussels sprouts on the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) into human urine was investigated. Ten non-smoking volunteers (five males and five females) were randomly assigned to two groups. Five volunteers started on a diet of 300 g of glucosinolate-free vegetables whereas the other five consumed 300 g of Brussels sprouts per day. After 1 week dietary regimes were reversed. Levels of 8-oxodG in 24-h urine samples were measured by high-performance liquid chromatography. In four of five males a reduction in 8-oxodG was found, whereas in the fifth male the 8-oxodG excretion was high in the control period and was even much higher in the sprouts period. In females no effect of consumption of Brussels sprouts on excretion of 8-oxodG was found. Our previous and present findings support the results of epidemiologic studies that consumption of brassica vegetables may diminish cancer risk.
Verhagen
H
H
TNO Nutrition and Food Research Institute, Zeist, The Netherlands. verhagen@voeding.tno.nl
de Vries
A
A
Nijhoff
W A
WA
Schouten
A
A
van Poppel
G
G
Peters
W H
WH
van den Berg
H
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002851:Chromatography, High Pressure Liquid; D018592:Cross-Over Studies; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004032:Diet; D005260:Female; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D012737:Sex Factors; D014675:Vegetables
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine