title: Isolation and toxicity of Cylindrospermopsis raciborskii from an ornamental lake.
abstract: In Australia, the tropical/subtropical cyanobacterium Cylindrospermopsis raciborskii forms substantial blooms in some drinking water supply reservoirs, rivers and recreational water bodies during the warmer months of the year. This paper describes the isolation, culture and toxicity characterisation of Cylindrospermopsis from a water bloom in a small lake in NSW, Australia. The cyanobacterium grew as straight trichomes terminating with a characteristic heterocyst. The toxic alkaloid cylindrospermopsin was separated and identified by high-performance liquid chromatography at a concentration of 5.5 mg/g dry cells, 0.026 pg/cell. Intraperitoneal injection of sonicated cells caused liver, kidney, intestinal and lung damage, with an LD50 of 52 mg cells/kg mouse body weight at 24 hr, and 32 mg/kg at 7 days. The 24 hr mouse toxicity is not consistent with previous studies using pure cylindrospermopsin, and is suggestive of other toxic compounds in this isolate.
authors.affiliation: AWT EnSight, West Ryde, NSW, Australia.
authors.forename: P R
authors.initials: PR
authors.lastname: Hawkins
authors.forename: N R
authors.initials: NR
authors.lastname: Chandrasena
authors.forename: G J
authors.initials: GJ
authors.lastname: Jones
authors.forename: A R
authors.initials: AR
authors.lastname: Humpage
authors.forename: I R
authors.initials: IR
authors.lastname: Falconer
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification* / Q000633:toxicity*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D005618:Fresh Water / Q000737:chemistry*; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D051379:Mice
chemical_list: D000470:Alkaloids