An improved primer for PCR amplification of mitochondrial DNA in a variety of insect species.
A PCR primer from the mitochondrial COI gene is described that enhances the amplification of the COI-COII region of insect mtDNA. When used in conjunction with a primer from the COII gene identified by R. Crozier, a 1600-1700 bp segment is amplified in nine species of insects representing the orders Lepidoptera, Diptera, Coleoptera and Hymenoptera.
Roehrdanz
R L
RL
United States Department of Agriculture, Biosciences Research Laboratory, Fargo, North Dakota 58105, USA.
D000818:Animals; D017931:DNA Primers; D004272:DNA, Mitochondrial; D017344:Genes, Insect; D007313:Insecta / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004272:DNA, Mitochondrial