title: Nitric oxide acts as a retrograde messenger during long-term potentiation in cultured hippocampal neurons.
abstract: We examined long-term potentiation (LTP) at synapses between hippocampal neurons in dissociated cell culture following presynaptic, postsynaptic, or extracellular application of a nitric oxide (NO) scavenger, an inhibitor of NO synthase, and a membrane-impermeant NO donor that releases NO only upon photolysis with UV light. Our results indicate that NO is produced in the postsynaptic neuron, travels through the extracellular space, and acts directly in the presynaptic neuron to produce long-term potentiation, supporting the hypothesis that NO acts as a retrograde messenger during LTP.
authors.affiliation: Center for Neurobiology and Behavior, Columbia University, New York, NY, USA.
authors.forename: O
authors.initials: O
authors.lastname: Arancio
authors.forename: V
authors.initials: V
authors.lastname: Lev-Ram
authors.forename: R Y
authors.initials: RY
authors.lastname: Tsien
authors.forename: E R
authors.initials: ER
authors.lastname: Kandel
authors.forename: R D
authors.initials: RD
authors.lastname: Hawkins
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000502:physiology*; D017774:Long-Term Potentiation / Q000187:drug effects; D009211:Myoglobin / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009569:Nitric Oxide / Q000494:pharmacology / Q000502:physiology*; D010782:Photolysis; D015290:Second Messenger Systems; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D014466:Ultraviolet Rays; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D009211:Myoglobin; C017595:oxymyoglobin; D019323:omega-N-Methylarginine; D009569:Nitric Oxide