Isolation of a germline-transmissible embryonic stem (ES) cell line from C3H/He mice.
We have isolated three embryonic stem (ES) cell lines from C3H/He mice using mouse STO cells as a feeder layer. One ES cell line (H-1) was male, and two (H-2 and H-3) were female, as determined by polymerase chain reaction, in situ hybridization, and karyotype analyses. All were immunocytochemically reactive with a C3H strain-specific antibody. Injection of cells from the female ES H-3 line into C57BL/6 blastocysts yielded four chimeras with slight coat color chimerism. All chimeras were male, and as expected, no germline-transmission was observed. By contrast, when male ES H-1 cells were injected into the perivitelline space of 8-cell C57BL/6 embryos, one male mouse with overt coat color chimerism was recovered, and it produced ES H-1-derived offspring exclusively. This germline-transmissible C3H/He cell line represents a novel addition to those ES lines currently employed for gene manipulation studies of development.
Kitani
H
H
National Institute of Animal Health, Ibaraki, Japan.
Takagi
N
N
Atsumi
T
T
Kawakura
K
K
Imamura
K
K
Goto
S
S
Kusakabe
M
M
Fukuta
K
K
D000818:Animals; D002460:Cell Line; D002678:Chimera; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D018095:Germ-Line Mutation; D017403:In Situ Hybridization; D007621:Karyotyping; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D012730:Sex Chromosomes; D013234:Stem Cells / Q000166:cytology*