Simultaneous detection of multiple gene expression in mouse and human individual preimplantation embryos.
OBJECTIVE
To detect simultaneously multiple gene expression in mouse and human individual embryos by reverse transcriptase-polymerase chain reaction.


METHODS
Transcripts involved in the insulin-like growth factor (IGF) system were detected in mouse and human preimplantation embryos.


METHODS
An academic teaching hospital.


METHODS
Transcripts of the IGF family genes.


RESULTS
In the mouse, genes are expressed differentially and messenger RNA transcripts of maternal origin in nonfertilized ova decline gradually until the initiation of the embryonic genome transcription. Insulin-like growth factor-binding protein-2 (IGFBP)-2, -3, -4, and beta-actin transcripts appear to be initiated at the two- to four-cell stage, whereas IGFBP-1, -5, and -6 transcripts are initiated at later stages. Transcription, once initiated, appears to continue through to the blastocyst stage. In humans, almost all genes of the IGF system were expressed in preimplantation embryos. This is the first report of the assessment of IGF family transcripts in individual embryos, and introduces a novel method for research and clinical diagnosis of preimplantation embryos.
Liu
H C
HC
Center for Reproductive Medicine and Infertility, Cornell University Medical College, New York, New York 10021, USA.
He
Z Y
ZY
Tang
Y X
YX
Mele
C A
CA
Veeck
L L
LL
Davis
O
O
Rosenwaks
Z
Z
D000199:Actins / Q000032:analysis / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D011972:Receptor, Insulin / Q000235:genetics; D017451:Receptors, Somatomedin / Q000032:analysis / Q000235:genetics*; D012732:Sex Determination Analysis; D013002:Somatomedins / Q000032:analysis / Q000235:genetics*
D000199:Actins; D017931:DNA Primers; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D017451:Receptors, Somatomedin; D013002:Somatomedins; D011972:Receptor, Insulin