title: A new sensitive cell culture test for the assessment of pesticide toxicity.
abstract: A new, simple and sensitive cell culture test for the determination of cytotoxicity of pesticides is described. This test is based on assessment of the growth inhibitory effect of pesticides on the rapidly growing mouse hybridoma cell line 1E6 cultivated in a defined serum-free medium (RPMI-SR3). In addition the cytotoxicity in serum-containing medium was investigated. The results showed that the sensitivity of 1E6 cell towards the toxicity of the pesticides; carbofuran, cypermethrin, lindane, glyphosate and 2,4-D was considerably higher in the serum-free medium than in serum-containing medium. Also IC50 values of these pesticides were compared with LD50 values obtained from the literature. The ratio between the in vitro IC50 and the in vivo LD50 showed that the present cell culture test determine the toxicity of low levels of pesticides with much higher sensitivity than tests based on using animals.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Tromsö, Norway.
authors.forename: K
authors.initials: K
authors.lastname: Bertheussen
authors.forename: M I
authors.initials: MI
authors.lastname: Yousef
authors.forename: Y
authors.initials: Y
authors.lastname: Figenschau
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity; D000826:Animal Testing Alternatives; D000818:Animals; D002235:Carbofuran / Q000633:toxicity; D002452:Cell Count; D002460:Cell Line; D002800:Cholinesterase Inhibitors / Q000633:toxicity; D003470:Culture Media; D016895:Culture Media, Serum-Free; D005998:Glycine / Q000031:analogs & derivatives / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D001556:Hexachlorocyclohexane / Q000633:toxicity; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D051379:Mice; D011722:Pyrethrins / Q000633:toxicity; D013329:Structure-Activity Relationship; D000097797:Glyphosate
chemical_list: D015084:2,4-Dichlorophenoxyacetic Acid; D002235:Carbofuran; D002800:Cholinesterase Inhibitors; D003470:Culture Media; D016895:Culture Media, Serum-Free; D005998:Glycine; D006540:Herbicides; D001556:Hexachlorocyclohexane; D007306:Insecticides; D011722:Pyrethrins; D000097797:Glyphosate; C017160:cypermethrin