Detection of matrix metalloproteinase activity in human pancreatic cancer.
Destruction of the basement membrane (BM) is mandatory for tumor spread, and matrix metalloproteinases (MMPs) are known to be implicated in colon cancer invasion and metastasis by digesting type IV collagen, a main component of the BM. The current study analyzed the expression of MMP-2 and MMP-9 in pancreatic cancer tissues. Frozen specimens of pancreatic cancer (n = 10), a liver metastatic nodule from pancreatic cancer (n = 1), and normal pancreas (n = 3) were homogenized and analyzed by zymography. The activated form of MMP-9 (82 kDa) was detected in all of the normal and malignant tissues, while the activated form of MMP-2 (62 kDa) was detected in all of the pancreatic cancers and its metastatic tissue, but not in the normal pancreatic tissues. These results indicate that expression of the activated form of MMP-2 may be specific to pancreatic cancer, while that of MMP-9 may be unrelated to it.
Koshiba
T
T
First Department of Surgery, Kyoto University, Japan.
Hosotani
R
R
Wada
M
M
Fujimoto
K
K
Lee
J U
JU
Doi
R
R
Arii
S
S
Imamura
M
M
D014408:Biomarkers, Tumor / Q000032:analysis; D017364:Collagenases / Q000032:analysis*; D018093:Gelatinases / Q000032:analysis*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000556:secondary; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000032:analysis*; D010179:Pancreas / Q000201:enzymology; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000473:pathology
D014408:Biomarkers, Tumor; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9