Enzymatic syntheses of GlcNAc beta 1-2Man and Gal beta 1-4GlcNAc beta 1-2Man as components of complex type sugar chains.
GlcNAc beta 1-2Man and GlcNAc beta 1-6Man were synthesized using the reverse hydrolysis activity of beta-N-acetylglucosaminidase from both jack beans and Bacillus circulans. In turn, Gal beta 1-4GlcNAc beta 1-2Man and Gal beta 1-4GlcNAc beta 1-6Man were synthesized regioselectively using the transglycosylation activity of beta-galactosidase from Diplococcus pneumoniae and B. circulans, respectively. These di- and trisaccharides are important components of complex type sugar chains and will be used as intermediates in our synthetic studies.
Fujimoto
H
H
Meiji Institute of Health Science, Naruda Odawara, Japan.
Isomura
M
M
Miyazaki
T
T
Matsuo
I
I
Walton
R
R
Sakakibara
T
T
Ajisaka
K
K
D000118:Acetylglucosaminidase / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000138:chemical synthesis* / Q000378:metabolism*; D006031:Glycosylation; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D013296:Streptococcus pneumoniae / Q000201:enzymology; D014312:Trisaccharides / Q000138:chemical synthesis* / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D004187:Disaccharides; D014312:Trisaccharides; C106299:N-acetylglucosaminyl-1-2-mannopyranose; C106298:galactopyranosyl-1-4-N--acetylglucosaminyl-1-2-mannopyranose; D001616:beta-Galactosidase; D000118:Acetylglucosaminidase