Differential effects of isoliquiritigenin and YC-1 in rat aortic smooth muscle.
We investigated the effects of isoliquiritigenin and YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole) on tension in endothelial-free rat aortic rings precontracted with phenylephrine (3 microM). Both compounds induced a concentration-dependent relaxation (EC50 of YC-1 1.9 microM and of isoliquiritigenin 9.4 microM). The effects developed faster with YC-1 than with isoliquiritigenin, and the effects of YC-1 were potentiated by isoliquiritigenin (10 microM). 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (30 microM) inhibited the effect of YC-1, but not of isoliquiritigenin. These results suggest that the effects of YC-1 are due to stimulation of soluble guanylyl cyclase activity, whereas the effects of isoliquiritigenin are rather related to inhibition of phosphodiesterase activity.
Wegener
J W
JW
Pharmakologisches Institut der Universität Mainz, Germany.
Nawrath
H
H
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects / Q000378:metabolism; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology; D047188:Chalcones; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006162:Guanylate Cyclase / Q000378:metabolism; D007191:Indazoles / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D047188:Chalcones; D004791:Enzyme Inhibitors; D007191:Indazoles; D010726:Phosphodiesterase Inhibitors; D010975:Platelet Aggregation Inhibitors; C090937:3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole; D002599:Chalcone; C040920:isoliquiritigenin; D000449:Aldehyde Reductase; D006162:Guanylate Cyclase