Identification of a novel Krueppel-related zinc finger gene (ZNF184) mapping to 6p21.3.
cDNA selection and exon trapping were performed on cosmids mapping to a region 3 Mb distal to HLA-A. Analysis of resulting fragments indicated the presence of two zinc finger transcripts, and one of these was used to isolate a partial cDNA (ZNF184) from a placental library. The second transcript contained additional sequence of the 5' end of the gene, extending the sequence to 2678 bp. Sequence analysis indicates that ZNF184 is a classical Krueppel zinc finger with 19 highly conserved zinc finger motifs at the C-terminus and a Krueppel associated box at the N-terminus of the protein. This gene encodes a 3.2-kb transcript that is highly expressed in testis and expressed at a moderate to low level in all other tissues tested. This zinc finger gene maps to a region approximately 200 kb distal to the microsatellite marker D6S105 and approximately 300 kb proximal to D6S1260.
Goldwurm
S
S
The Queensland Institute of Medical Research, University of Queensland, Brisbane, Queensland, 4029, Australia. stefanoG@qimr.edu.au
Menzies
M L
ML
Banyer
J L
JL
Powell
L W
LW
Jazwinska
E C
EC
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D016335:Zinc Fingers / Q000235:genetics*