title: Functional transitions in myosin: role of highly conserved Gly and Glu residues in the active site.
abstract: It has been proposed from crystallographic comparisons [Fisher, A. J., Smith, C. A., Thoden, J. B., Smith, R., Sutoh, K., Holden, H. M., & Rayment, I. (1995) Biochemistry 34, 8960-8972] that in one of the important transitions of myosin head (M), M x ATP --> M x ADP x Pi, a rotation occurs in Gly468 (of chicken smooth muscle myosin). We find that mutation of this Gly to Ala does block the transition. Searching for proton acceptors in ATPase catalysis, we also find that mutation of a candidate (Glu470 of chicken smooth muscle myosin) blocks the transition. Interpretations of both findings are examined.
authors.affiliation: Department of Structural Analysis, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Onishi
authors.forename: M F
authors.initials: MF
authors.lastname: Morales
authors.forename: S
authors.initials: S
authors.lastname: Kojima
authors.forename: K
authors.initials: K
authors.lastname: Katoh
authors.forename: K
authors.initials: K
authors.lastname: Fujiwara
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D018698:Glutamic Acid / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry*; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000737:chemistry; D009130:Muscle, Smooth; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D015879:Myosin Subfragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009218:Myosins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D013050:Spectrometry, Fluorescence
chemical_list: D000199:Actins; D015879:Myosin Subfragments; D010710:Phosphates; D018698:Glutamic Acid; D000255:Adenosine Triphosphate; D009218:Myosins; D005998:Glycine