Changes in the urinary excretion level of 8-hydroxyguanine by exposure to reactive oxygen-generating substances.
A simple means of measuring of 8-hydroxyguanine (8-OHGua) levels in urine was developed. Rat and human urine samples were purified by means of strong cation exchange chromatography (Amberlite CG-120), followed by cellulose partition chromatography (Whatman CF-11). Thereafter, 8-OHGua was determined by means of high performance liquid chromatography (HPLC) using an electro-chemical detector. The level of 8-OHGua in rat urine increased by a factor of 2 to 4 after an intraperitoneal administration of 2-nitropropane (25 mg/kg), paraquat (11.3 mg/kg), or hydroquinone (11 mg/kg). On the other hand, the urine of smokers and persons exposed to air polluted with car exhaust also contained 1.9 and 3.8 fold more 8-OHGua, respectively, than that of control nonsmokers. These results indicated that the amount of 8-OHGua in urine is useful marker for monitoring the level of in vivo oxidative stress.
Suzuki
J
J
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Inoue
Y
Y
Suzuki
S
S
D000328:Adult; D000397:Air Pollution / Q000009:adverse effects; D000818:Animals; D015415:Biomarkers / Q000652:urine; D002845:Chromatography; D005609:Free Radicals / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D006873:Hydroquinones / Q000633:toxicity; D008297:Male; D009595:Nitroparaffins / Q000633:toxicity; D018384:Oxidative Stress; D010269:Paraquat / Q000633:toxicity; D011407:Propane / Q000031:analogs & derivatives / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012907:Smoking / Q000652:urine
D015415:Biomarkers; D005609:Free Radicals; D006873:Hydroquinones; D009595:Nitroparaffins; D017382:Reactive Oxygen Species; C024829:8-hydroxyguanine; D006147:Guanine; C016617:2-nitropropane; D010269:Paraquat; D011407:Propane; C031927:hydroquinone