Immunoelectron microscopic localization of the HNK-1 carbohydrate epitope in the anterior segment of pseudoexfoliation and normal eyes.
OBJECTIVE
To study the presence of the cell-adhesion related HNK-1 carbohydrate epitope in the anterior segment of pseudoexfoliation and normal eyes by immunoelectron microscopy.


METHODS
Anterior segment tissue of 6 autopsy eyes with pseudoexfoliation (PEX) syndrome (5 eyes without glaucoma and 1 with glaucoma), and 6 normal autopsy eyes without PEX syndrome were studied by an electron microscopic immunogold technique using a monoclonal antibody to the HNK-1 epitope.


RESULTS
In both normal and PEX eyes, the HNK-1 epitope could be immunolocalized to the basement membranes of both ciliary epithelia and posterior iris pigmented epithelium, to the lens capsule and zonular lamella, and to the pigmented epithelial cells of iris and ciliary body. Within the inner connective tissue layer of the ciliary body, the gold label was mainly associated with the periphery of elastic fibers and microfibrillar bundles. PEX material on the surfaces of posterior iris, ciliary body, anterior lens capsule, zonular fibers, and uveal part of the trabecular meshwork reacted strongly with the HNK-1 antibody. In contrast, PEX material accumulations within the iris stroma or the juxtacanalicular tissue of the trabecular meshwork showed only weak immunoreactivity, while PEX material in the conjunctiva was totally negative.


CONCLUSIONS
The wide distribution of the HNK-1 epitope in anterior segment tissues and its association with a variety of extracellular and cellular structures was ultrastructurally demonstrated. In PEX syndrome, the varying labelling density of PEX fibers indicates a deviating carbohydrate composition in different locations of the eye. The HNK-1 epitope might be involved in the adhesiveness of PEX deposits on intraocular surfaces.
Kubota
T
T
Department of Ophthalmology, Kyushu University, Fukuoka, Japan.
Schlötzer-Schrehardt
U
U
Inomata
H
H
Naumann
G O
GO
D000368:Aged; D018959:CD57 Antigens / Q000032:analysis*; D002924:Ciliary Body / Q000276:immunology / Q000648:ultrastructure; D003228:Conjunctiva / Q000276:immunology / Q000648:ultrastructure; D003315:Cornea / Q000276:immunology / Q000648:ultrastructure; D017889:Exfoliation Syndrome / Q000276:immunology* / Q000503:physiopathology; D006801:Humans; D007498:Iris / Q000276:immunology / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D014129:Trabecular Meshwork / Q000276:immunology / Q000648:ultrastructure
D018959:CD57 Antigens