Study on serum-free media for genetically engineered CHO cells producing prourokinase.
Based on the initial optimization of DMEM:F12(1:1), two kinds of serum-free media, 11G-SG-SFM and 11G-SE-SFM, were formulated by using orthogonal experiments for the growth of genetically engineered CHO cell line 11G and the production of recombinant prourokinase (pro-UK) in a suspension culture, respectively. Growth of 11G cells in 11G-SG-SFM was comparable with that observed in DMEM:F12 + 5% NBS and CHO-S-SFM. 11G cells grew slowly in 11G-SE-SFM, but secreted a relatively high level of pro-UK. The pro-UK productivity of 11G cells in 11G cells in 11G-SE-SFM reached 800 to 1,000 IU/10(6). d-1 and was about 80% and 20% higher than in DMEM:F12 + 5% NBS and CHO-S-SFM, respectively.
Chen
Z
Z
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China.
Wu
B
B
Jia
X
X
Liu
H
H
Xiao
C
C
D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D006224:Cricetinae; D016895:Culture Media, Serum-Free; D005818:Genetic Engineering; D011498:Protein Precursors / Q000096:biosynthesis; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics*
D016895:Culture Media, Serum-Free; D011498:Protein Precursors; D014568:Urokinase-Type Plasminogen Activator