Photooxidant injury to glucose-6-phosphate dehydrogenase-deficient erythrocytes with bilirubin as the sensitizer--an in vitro study.
To evaluate the hypothesis that glucose-6-phosphate dehydrogenase (G6PD)-deficient erythrocytes are more prone to photooxidant injury with bilirubin as the sensitizer, we compared the malonyl dialdehyde (MDA) levels and haematocrit values in 11 G6PD-deficient samples with a bilirubin: albumin molar ratio of 1:2 (10 mg% bilirubin, 2.5 g % albumin) photoexposed at 6 microW cm-2 nm-2 (420-460 nm), with 11 of control samples. Twelve hours after photoexposure the incremental changes in MDA levels (OD units) were higher in G6PD-deficient samples [mean (SE) = 0.230 (0.171) vs 0.075 (0.043), p < 0.01]. Antioxidant drugs may have a protective role while G6PD-deficient neonates undergo phototherapy.
Karthikeyan
G
G
Department of Pediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Narang
A
A
Majumdar
S
S
Shivani
K
K
D001663:Bilirubin / Q000097:blood*; D004912:Erythrocytes / Q000502:physiology*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000175:diagnosis*; D006400:Hematocrit; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007567:Jaundice, Neonatal / Q000628:therapy; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood; D010789:Phototherapy; D012709:Serum Albumin
D012709:Serum Albumin; D008315:Malondialdehyde; D001663:Bilirubin