title: Co-localization of beta-amyloid peptides, apolipoprotein E and glial markers in senile plaques in the prefrontal cortex of old rhesus monkeys.
abstract: Based on the homology of human and monkey amyloid precursor proteins and the derived beta-amyloid peptides (A beta) the investigation of brains from old monkeys might be useful for the understanding of beta-amyloidosis in the aetiology of Alzheimer's disease. In the present study, the prefrontal cortex, which is known to be highly susceptible to the deposition of A beta, was screened for the occurrence of senile plaques in perfused tissue of aged rhesus monkeys (Macaca mulatta). A beta deposits were immunocytochemically detected in five of six macaques aged about 28 years. Differently N-terminal truncated A beta species in the senile plaques were simultaneously detected by a carbocyanine double fluorescence method applying the bright red fluorescent Cy3 and the novel green fluorescent Cy2. In a few cases, immunoreactivity for the shortened fragment containing the amino acids 17-42 (A beta(17-42); p3 fragment with a molecular weight of 3 kDa) was demonstrated in deposits apparently devoid of A beta(8-17). Senile plaques were further characterized by carbocyanine double labelling of A beta and astrocytes, microglia and apolipoprotein E.
authors.affiliation: Paul Flechsig Institute for Brain Research, University of Leipzig, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Härtig
authors.forename: G
authors.initials: G
authors.lastname: Brückner
authors.forename: C
authors.initials: C
authors.lastname: Schmidt
authors.forename: K
authors.initials: K
authors.lastname: Brauer
authors.forename: G
authors.initials: G
authors.lastname: Bodewitz
authors.forename: J D
authors.initials: JD
authors.lastname: Turner
authors.forename: V
authors.initials: V
authors.lastname: Bigl
mesh_terms: D000375:Aging / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D015415:Biomarkers; D002232:Carbocyanines; D004396:Coloring Agents; D008253:Macaca mulatta; D008297:Male; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism* / Q000473:pathology; D014018:Tissue Distribution
chemical_list: D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D015415:Biomarkers; D002232:Carbocyanines; D004396:Coloring Agents; D010446:Peptide Fragments