Mapping of peptidic epitopes of glycophorins A (GPA) and C (GPC) with peptides synthesized on plastic pins (Pepscan analysis).
The peptidic epitopes of 12 anti-GPA and 4 anti-GPC antibodies were identified with the use of peptides synthesized on the pins. Most of the antibodies were specific for epitopes located in extracellular portion of glycophorins, and only 2 anti-GPA and 1 anti-GPC recognized epitopes in their C-terminal cytoplasmic tails. The extracellular GPA epitopes were located in two regions of the polypeptide chain, within a.a. residues 38-44 and 49-58.
Waśniowska
K
K
Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
Jaśkiewicz
E
E
Czerwiński
M
M
Syper
D
D
Lisowska
E
E
D000911:Antibodies, Monoclonal; D018604:Epitope Mapping; D006021:Glycophorins / Q000276:immunology*; D006031:Glycosylation; D006801:Humans; D010449:Peptide Mapping; D010455:Peptides / Q000138:chemical synthesis*; D010969:Plastics
D000911:Antibodies, Monoclonal; D006021:Glycophorins; D010455:Peptides; D010969:Plastics