title: Growth of Escherichia coli in acetate as a sole carbon source is inhibited by ankyrin-like repeats present in the 2',5'-linked oligoadenylate-dependent human RNase L enzyme.
abstract: Expression of low levels of the 2',5'-linked oligoadenylate-dependent human RNase L, an enzyme induced by interferons, is highly toxic in Escherichia coli. This protein contains an ankyrin domain responsible for RNase L toxicity. The only known ORF in E. coli containing ankyrin repeats is yjaC in the acetate metabolic cluster. We have investigated if expression of mutant forms of RNase L interfere with metabolism of acetate in E. coli. Our findings demonstrate that E. coli expressing RNase L ankyrin repeats is unable to grow in medium containing acetate as the sole carbon source, while it can grow when expressing other domains of the protein. This defect correlates with a severe decrease in the levels of induction of enzymes in the glyoxylate bypass.
authors.affiliation: Centro Nacional de Biotechnología, CSIC, Campus Universidad Autónoma, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Díaz-Guerra
authors.forename: M
authors.initials: M
authors.lastname: Esteban
authors.forename: J L
authors.initials: JL
authors.lastname: Martínez
mesh_terms: D000085:Acetates / Q000378:metabolism / Q000494:pharmacology*; D017487:Ankyrins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D005947:Glucose / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D006801:Humans; D007522:Isocitrate Lyase / Q000378:metabolism; D008292:Malate Synthase / Q000378:metabolism; D010957:Plasmids; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D019289:Pyruvic Acid / Q000494:pharmacology
chemical_list: D000085:Acetates; D017487:Ankyrins; D001426:Bacterial Proteins; D018698:Glutamic Acid; D019289:Pyruvic Acid; D008292:Malate Synthase; D011494:Protein Kinases; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D007522:Isocitrate Lyase; D005947:Glucose