Density dependent change of myristoylated proteins in C3H10T1/2 fibroblasts and their transformants.
We have examined the pattern of protein myristoylation in C3H10T1/2 fibroblasts during cell growth. During the growing phase of 10T1/2 cells, several proteins were radiolabelled with [3H]myristate, and among them proteins with molecular masses of 22, 35, a doublet of 42-45 and 67 kDa were labelled predominantly. The extent of myristoylation in each of these proteins changed with cell density. The amount of radioactivity incorporated into the 22 kDa protein in 10T1/2 cells decreased with increasing cell density and remained at a low level during the stationary phase. In contrast, the incorporation into the 67 kDa protein increased parallel to cell density. The density-dependent change of myristoylation was not observed in any of the transformants of 10T1/2 cells thus far examined. The 67 kDa protein was identified as MARCKS (myristoylated alanine-rich C kinase substrate) by immunoprecipitation with an anti-MARCKS antibody. By Western blot analysis, we found that the amount of MARCKS in 10T1/2 cells increased significantly analogous with cell density. Therefore, it is possible that MARCKS and the 22 kDa protein play a role in contact-mediated cell signalling in 10T1/2 cells, but the mechanism is lost in transformed cells.
Wada
E
E
Institute of Medical Science, University of Tokyo, Japan.
Sakiyama
H
H
Nakamura
M
M
Kanegasaki
S
S
D000818:Animals; D002450:Cell Communication / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D051379:Mice; D008970:Molecular Weight; D019814:Myristic Acid; D009227:Myristic Acids / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011493:Protein Kinase C / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D014316:Tritium; D014965:X-Rays
D047908:Intracellular Signaling Peptides and Proteins; C000619711:Marcks protein, mouse; D008565:Membrane Proteins; D009227:Myristic Acids; D011506:Proteins; D019814:Myristic Acid; D014316:Tritium; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011493:Protein Kinase C