Isolation of a cell surface component of Helicobacter pylori that binds H type 2, Lewis(a), and Lewis(b) antigens.
OBJECTIVE
Individuals of blood group O and nonsecretors of ABO blood group antigens are more susceptible to peptic ulcers. The aim of this study was to determine if blood group antigens associated with group O or secretor status are epithelial cell receptors for Helicobacter pylori.


METHODS
Bacterial binding and binding of monoclonal antibodies to H type 2, Lewis(a), and Lewis(b) to Kato III, buccal epithelial, and gastric mucosal cells were shown by flow cytometry. Bacterial outer membrane proteins eluted from H type 2, Lewis(a), or Lewis(b) were shown by polyacrylamide gel electrophoresis.


RESULTS
Kato III and human epithelial cells bound each monoclonal antibody; O cells bound more anti-H type 2 (P < 0.05). Binding indices for H. pylori correlated with those for anti-H type 2 (P < 0.005) and anti-Lewis(b) (P < 0.001) but not anti-Lewis(a). A 61-kilodalton protein was eluted from H type 2, Lewis(a), or Lewis(b).


CONCLUSIONS
Our results indicate that H type 2 is an important receptor for the 61-kilodalton bacterial adhesin, partly explaining increased susceptibility of individuals of blood group O to ulcers. Lewis(b) binds H. pylori more efficiently than Lewis(a). If these interactions occur in vivo, lack of Lewis(b) in mucosal fluids of nonsecretors may contribute to colonization by H. pylori.
Alkout
A M
AM
Department of Medical Microbiology, University of Edinburgh, Scotland.
Blackwell
C C
CC
Weir
D M
DM
Poxton
I R
IR
Elton
R A
RA
Luman
W
W
Palmer
K
K
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000378:metabolism*; D001710:Biotin; D002460:Cell Line; D002610:Cheek; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism; D016480:Helicobacter pylori / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007519:Isoantigens / Q000378:metabolism*; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism; D012016:Reference Values; D013272:Stomach Diseases / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; C022863:H antigen; D007519:Isoantigens; D007983:Lewis Blood Group Antigens; D008565:Membrane Proteins; D001710:Biotin