Rapid solid-phase extraction/derivatization system for sample preparation and gas chromatographic/mass spectrometric determination of drugs in human urine.
A novel automatic system has been developed for the rapid solid-extraction and derivatization of different drugs and their metabolites from urine, prior to further analysis. Manual injection of underivatized/derivatized drugs in a GC/MS system, in which the quadrupole mass filter was operated conventionally, provided high sensitivity and selectivity at the selected m/z value. The precision (2.1-6.8%) is acceptable and the overall recovery from urine was determined to be ca. 95%. The effectiveness of this approach is demonstrated with data obtained from different urine samples which had tested positively.
Cárdenas
S
S
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Gallego
M
M
Valcárcel
M
M
D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods; D014556:Urine / Q000737:chemistry
D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations