31P NMR identification of metabolites and pH determination in the cyanobacterium Synechocystis sp. PCC 6308.
The identity of a number of phosphorus-containing metabolites present in Synechocystis sp. PCC 6308 has been confirmed by 31P NMR spectroscopy. The presence of D-ribulose 1,5-bisphosphate (RuBP); DL-glyceraldehyde 3-phosphate (GlyP); D(-)3-phosphoglyceric acid (3PGA); D-ribulose 5-phosphate (Ru5P);6-phosphogluconic acid (6PGA); phosphoenolpyruvate (PEP); inorganic phosphate (Pi); uridine diphosphoglucose (UDPG); ADP and ATP were demonstrated by the pH dependence of their 31P NMR chemical shifts in spectra of perchloric acid cell extracts. Intracellular pH of cells was determined to be 7.5-7.7.
Lawrence
B A
BA
Department of Biology, Wellesley College, Wellesley, MA 02181, USA.
Polse
J
J
DePina
A
A
Allen
M M
MM
Kolodny
N H
NH
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000458:Cyanobacteria / Q000378:metabolism*; D005942:Gluconates / Q000378:metabolism; D005986:Glyceraldehyde 3-Phosphate / Q000378:metabolism; D005988:Glyceric Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D012274:Ribulosephosphates / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D005942:Gluconates; D005988:Glyceric Acids; D010710:Phosphates; D012274:Ribulosephosphates; D005986:Glyceraldehyde 3-Phosphate; C001933:ribulose-1,5 diphosphate; D010758:Phosphorus; C031524:ribulose 5-phosphate; D000244:Adenosine Diphosphate; D010728:Phosphoenolpyruvate; C005156:3-phosphoglycerate; D000255:Adenosine Triphosphate; D014532:Uridine Diphosphate Glucose; C008884:6-phosphogluconic acid