Recovery of mucin content in surface layer of rat gastric mucosa after HCl-aspirin-induced mucosal damage.
Quantitative changes in mucin (mucus glycoprotein) in different layers of rat gastric mucosa after mucosal damage induced by acidified acetylsalicylic acid (HCl-aspirin; 0.15N HCl, 20-200 mg acetylsalicylic acid/kg body weight) were studied. More than 50 mg/kg HCl-aspirin led to a significant increase in macroscopic gastric injury (expressed as ulcer index) at 3 h, compared with control (no aspirin) and there was a significant recovery at 7 h. Three h after dosing with 50 mg/kg acidified aspirin, there was superficial mucosal damage and decreased mucin content in the surface mucosal layer. Mucin production recovered 7 h after the administration of 50 mg/kg acidified aspirin. Doses of acidified aspirin higher than 100 mg/kg decreased mucin content in the surface and deep corpus mucosal layers and no recovery was seen 7 h after the administration. Physiological damage after the administration of 50 mg/kg HCl-aspirin was limited mainly to surface epithelial mucus cells. An experimental model in which superficial erosion was induced in rat gastric mucosa was established with low-dose HCl-aspirin.
Sakai
T
T
Department of Internal Medicine, School of Medicine, Kitasato University, Kanagawa, Japan.
Ishihara
K
K
Saigenji
K
K
Hotta
K
K
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D001241:Aspirin / Q000009:adverse effects*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D006851:Hydrochloric Acid / Q000009:adverse effects; D006863:Hydrogen-Ion Concentration; D008297:Male; D009077:Mucins / Q000187:drug effects / Q000378:metabolism*; D009093:Mucus / Q000737:chemistry / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000378:metabolism; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009077:Mucins; D006851:Hydrochloric Acid; D001241:Aspirin