title: Flow cytometric DNA-heterogeneity in paraffin-embedded endometrial cancer.
abstract: To evaluate the degree of intratumoral DNA ploidy heterogeneity in endometrial carcinoma, the authors examined curettage specimens from 30 patients with clinical stage I and II endometrial carcinoma. The curettage material was obtained before the onset of treatment. A representative sample from each tumour was chosen and a 50 microm section was cut. The paraffin block of tumor was then divided into 4 equal parts and a 50 microm section was cut from each part. All tumour samples were analysed separately by flow cytometry. DNA ploidy heterogeneity was noted in 5/29 cases (17%). In three cases DNA-aneuploid stem cell lines were found only among the 4-part sections and were not detected when the whole tumor section was analysed.
authors.affiliation: Department of Gynecologic Oncology, University Hospital, Linkoping, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Rosenberg
authors.forename: S
authors.initials: S
authors.lastname: Wingren
authors.forename: C
authors.initials: C
authors.lastname: Guerrieri
mesh_terms: D000368:Aged; D004273:DNA, Neoplasm / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008875:Middle Aged; D011003:Ploidies
chemical_list: D004273:DNA, Neoplasm