Purification and characterization of ferritin from alfalfa seeds.
Ferritin from alfalfa (Medicago sativa) seeds was isolated, purified, and characterized. The apparent molecular mass of the native protein was found to be 560 kDa. Electrophoresis in denaturing gradient polyacrylamide-SDS gels revealed subunits of 28-26.5 kDa. The average iron cores were 4 nm in diameter and contained about 1400 iron atoms, with an iron-to-phosphorus ratio of 4:1. N-terminal amino acid sequencing of the 28 kDa subunit revealed close homology with other plant proteins. Immunochemical analysis using polyclonal antibodies raised against pea-seed ferritin has confirmed, in agreement with previous reports, that plant proteins share common epitopes.
Barceló
F
F
Departamento de Biología Fundamental y Ciencias de la Salud, Universidad de las Islas Baleares, Palma de Mallorca, Spain.
Miralles
F
F
Otero Areán
C
C
D000595:Amino Acid Sequence; D005293:Ferritins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D000455:Medicago sativa / Q000737:chemistry* / Q000235:genetics; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
D010940:Plant Proteins; D005293:Ferritins