title: Characterisation of recombinant isoforms of birch pollen allergen Bet v 1.
abstract: Three isoforms of the major birch pollen allergen, Bet v, 1 from Betula verrucosa have been expressed as recombinant proteins in E. coli and purified. The immunochemical properties of recombinant isoforms (rBet v 1) differed on immunoblots when compared using Mabs and birch pollen allergic patients serum IgE. 2-D gel analysis showed that recombinant isoforms with different epitope structure can focus under the same protein spot after electrophoresis. The structure of conformational epitopes can be distorted by amino acid substitutions even when T-cell epitopes are not affected as judged by T-cell proliferation studies.
authors.affiliation: ALK Laboratories, Hørsholm, Denmark.
authors.forename: M D
authors.initials: MD
authors.lastname: Spangfort
authors.forename: H
authors.initials: H
authors.lastname: Ipsen
authors.forename: S H
authors.initials: SH
authors.lastname: Sparholt
authors.forename: S
authors.initials: S
authors.lastname: Aasmul-Olsen
authors.forename: P
authors.initials: P
authors.lastname: Osmark
authors.forename: F M
authors.initials: FM
authors.lastname: Poulsen
authors.forename: M
authors.initials: M
authors.lastname: Larsen
authors.forename: E
authors.initials: E
authors.lastname: Mørtz
authors.forename: P
authors.initials: P
authors.lastname: Roepstorff
authors.forename: J N
authors.initials: JN
authors.lastname: Larsen
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D015180:Electrophoresis, Gel, Two-Dimensional; D007527:Isoenzymes / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000276:immunology; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014197:Trees
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D007527:Isoenzymes; D010936:Plant Extracts; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C061312:Bet v 1 allergen, Betula