Structural analysis of the oligosaccharide-alditols released by reductive beta-elimination from oviducal mucins of Rana temporaria.
The carbohydrate chains of the mucins which constitute the jelly coat surrounding the eggs of Rana temporaria were released by alkaline borohydride treatment. Neutral and acidic oligosaccharide-alditols were purified by ion-exchange chromatography and HPLC. From the structural analysis, based upon 1H and 13C-NMR spectroscopy in combination with MALDI-TOF, the following glycan units are proposed.
Maes
E
E
Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille (Flanders-Artois), Villeneuve d'Ascq, France.
Florea
D
D
Delplace
F
F
Lemoine
J
J
Plancke
Y
Y
Strecker
G
G
D000818:Animals; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry*; D010057:Oviducts / Q000378:metabolism*; D011896:Rana temporaria / Q000502:physiology*; D013402:Sugar Alcohols / Q000737:chemistry*
D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols