Nucleotide sequence analysis of two virulence-associated genes in Listeria monocytogenes serotype 1/2b and comparison with the same genes in other serotypes important in human disease.
The nucleotide sequences of the hly and plcA genes (encoding listeriolysin, a thiol-activated cytolysin and a non-specific phospholipase C, respectively) were determined for two strains of Listeria monocytogenes serotype 1/2b. The deduced amino acid sequences for listeriolysin were identical for the two strains and identical to that of listeriolysin from serotype 4b. The two serotype 1/2b strains differed in one amino acid (histidine vs asparagine) in the deduced amino acid sequence for phosopholipase C.
Vines
A
A
Clark Atlanta University, Atlanta, Georgia, USA.
Swaminathan
B
B
D003001:Cloning, Molecular; D003603:Cytotoxins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D008089:Listeria monocytogenes / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D010738:Type C Phospholipases / Q000235:genetics*
D003603:Cytotoxins; D017931:DNA Primers; D004269:DNA, Bacterial; D010738:Type C Phospholipases