Induction of cyclooxygenase-2 in the brain by cytokines.
The present study revealed that (1) a pyrogenic dose of IL-1 beta induced COX-2 mRNA in the brain vasculature and leptomeninges, and (2) the cells positive for COX-2 mRNA in the blood vessels were endothelial cells that possess receptors for IL-1. These results imply that circulating IL-1 beta acts on its receptor on the endothelial cells of the brain vasculature to induce COX-2 mRNA, which is possibly responsible for the elevated level of PGE2 seen during fever. In this sense, the endothelial cells in the brain vasculatures seem to play a role as an interface between the blood borne substance and the brain. The nature of the COX-2 mRNA-positive cells in the leptomeninges will be identified in our future study.
Cao
C
C
Department of Neuroscience, Osaka Bioscience Institute, Japan. cao@obi.or.jp
Matsumura
K
K
Watanabe
Y
Y
D000818:Animals; D001921:Brain / Q000201:enzymology*; D001931:Brain Mapping; D051546:Cyclooxygenase 2; D004790:Enzyme Induction / Q000187:drug effects; D005334:Fever / Q000503:physiopathology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D007375:Interleukin-1 / Q000494:pharmacology*; D007527:Isoenzymes / Q000096:biosynthesis*; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D011749:Pyrogens / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017472:Receptors, Interleukin-1 / Q000502:physiology
D007375:Interleukin-1; D007527:Isoenzymes; D011749:Pyrogens; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D018799:Intercellular Adhesion Molecule-1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases