title: Lipids may not be involved in the recognition of apocytochrome C during its transportation.
abstract: It was shown that apocytochrome c was less insertion into monolayers from outer mitochondrial membrane lipids than into those from microsomal membrane lipids; the alpha-helix content of apocytochrome c induced by small unilamellar vesicles prepared from outer mitochondrial membrane lipids was less than by those from microsomal membrane lipids; the import efficiency of apocytochrome c into large unilamellar vesicles from outer mitochondrial membrane lipids was also lower than into those from microsome membrane lipids. No specific affinity between apocytochrome c and outer mitochondrial membrane lipids could be found. Import of apocytochrome c across the intact mitochondria, sealed outer mitochondrial membrane and microsome membrane vesicles was compared. Results showed that apocytochrome c was accumulated only in mitochondria, but not the other two kinds of vesicles.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, China.
authors.forename: Q F
authors.initials: QF
authors.lastname: Ma
authors.forename: F Y
authors.initials: FY
authors.lastname: Yang
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000378:metabolism*; D001692:Biological Transport; D002462:Cell Membrane; D003574:Cytochrome c Group / Q000378:metabolism*; D045304:Cytochromes c; D050356:Lipid Metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D051381:Rats; D013552:Swine
chemical_list: D001059:Apoproteins; D003574:Cytochrome c Group; D045304:Cytochromes c