title: Hepatic adenine nucleotides and microsomal cholesterol 7 alpha-hydroxylase activity in the obstructed and freely draining lobes of the liver after selective bile duct obstruction.
abstract: BACKGROUND
  The effect of selective bile duct obstruction (SBDO) on hepatic reserve function of the bile duct obstructed (BDO) and nonobstructed freely draining (FD) lobes of the liver is obscure.
  
  
  METHODS
  The bile duct branches draining from the left lateral and median lobes of the liver were ligated for 4 and 10 days in rats, and hepatic reserve functions in BDO and FD lobes were assessed by microsomal cholesterol 7 alpha-hydroxylase activities and by hepatic adenine nucleotides and energy charge levels. The values were compared with those in the sham-operated control liver. Cholesterol 7 alpha-hydroxylase activities were determined by gas-liquid chromatography--mass spectrometry, and hepatic adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) levels with high-pressure liquid chromatography.
  
  
  RESULTS
  The histological examination of the BDO lobes showed proliferation and formation of new bile ductules and fibrous connective tissues linking portal areas. Microsomal cholesterol 7 alpha-hydroxylase activities, hepatic energy charge and each adenine nucleotide level did not differ between FD and BDO lobes, and the values were similar to those in the sham-operated liver.
  
  
  CONCLUSIONS
  Selective bile duct obstruction shows no adverse effects on microsomal and mitochondrial functions in both the BDO and FD lobes of the liver.
authors.affiliation: Department of Surgery 1, Kyushu University Faculty of Medicine, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Chijiiwa
authors.forename: Y
authors.initials: Y
authors.lastname: Hachiya
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: I
authors.initials: I
authors.lastname: Makino
authors.forename: T
authors.initials: T
authors.lastname: Naito
authors.forename: M
authors.initials: M
authors.lastname: Komura
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D001652:Bile Ducts / Q000473:pathology / Q000601:surgery; D001835:Body Weight; D002779:Cholestasis / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008111:Liver Function Tests; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000227:Adenine Nucleotides; D002790:Cholesterol 7-alpha-Hydroxylase