Immobilization of enzymes on methacrylamide-based copolymers.
A series of reactive polymeric supports for the covalent binding of enzymes was prepared from methacrylamide in conjunction with various copolymerized monomers. In all cases the specific surface area of the prepared copolymer particles increased with the ratio of crosslinker to monomer. The polymers containing oxirane and aldehyde groups were promising for direct binding of beta-galactosidase, whereas the supports containing amino groups also qualified for enzyme immobilization with glutaraldehyde.
Liao
W H
WH
Department of Chemical Engineering, National Chung Cheng University, Chiayi, Taiwan, ROC.
Lee
W C
WC
D000178:Acrylamides; D001236:Aspergillus oryzae; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D011108:Polymers; D013696:Temperature; D001616:beta-Galactosidase
D000178:Acrylamides; D004800:Enzymes, Immobilized; D011108:Polymers; D001616:beta-Galactosidase; C045985:methacrylamide