Various fructose-1,6-bisphosphatase mRNAs in mouse brain, liver, kidney and heart.
The mouse fructose-1,6-bisphosphatase (FBPase) cDNA was previously cloned from testicular teratocarcinoma cultured cells (F9 cells). Using this published nucleotide sequence four primer sets were defined and used to amplify FBPase transcript from cerebral cortex, heart, kidney, liver and testis of male C57B1/6 mice. Only one primer set was efficient in all total RNA prepared from the various tissues. The restriction maps of these RNA amplification products suggested the existence of three different FBPase transcripts; this was confirmed by the nucleotide sequences of the FBPase transcripts and by the deduced amino acid sequences. These data are consistent with the existence of three different FBPase genes. This may be relevant in neurological disease in which abnormalities of brain glucose metabolism are involved.
Cloix
J F
JF
UPR CNRS 9074, Institut de Transgénose CNRS, Orléans, France.
Beaulieu
E
E
Hevor
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology*; D017931:DNA Primers; D006597:Fructose-Bisphosphatase / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000737:chemistry; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013724:Teratoma; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D007527:Isoenzymes; D012333:RNA, Messenger; D006597:Fructose-Bisphosphatase