title: Natural anti-proteases in rainbow trout, Oncorhynchus mykiss and brook charr, Salvelinus fontinalis and the in vitro neutralization of fish alpha 2-macroglobulin by the metalloprotease from the pathogenic haemoflagellate, Cryptobia salmositica.
abstract: Natural anti-proteases (alpha 1-protease inhibitor (alpha 1-PI; alpha 1-antitrypsin) and alpha 2-macroglobulin (alpha 2-M)) were found in the blood of rainbow trout, Oncorhynchus mykiss and brook charr, Salvelinus fontinalis. The alpha 2-M inhibited Cryptobia salmositica proteases and was significantly higher in brook charr than in rainbow trout. Under in vitro conditions it took longer for the same number of parasites to neutralize the alpha 2-M in charr than in trout blood. The haemolysis which occurred when C. salmositica was incubated in the blood of rainbow trout was due to neutralization of alpha 2-M. This in vitro study also showed that it was the metalloprotease of C. salmositica that lysed red blood cells and the plasma of the two species of fishes initially prevented haemolysis by inhibiting the proteolytic activity. We suggest that the natural plasma alpha 2-M plays an important role in defence against cryptobiosis in fishes.
authors.affiliation: Department of Zoology, University of Guelph, Ontario, Canada.
authors.forename: X
authors.initials: X
authors.lastname: Zuo
authors.forename: P T
authors.initials: PT
authors.lastname: Woo
mesh_terms: D000818:Animals; D001769:Blood / Q000469:parasitology; D004912:Erythrocytes / Q000502:physiology; D005393:Fish Diseases; D006461:Hemolysis; D007113:Immunity, Innate; D016830:Kinetoplastida / Q000201:enzymology* / Q000472:pathogenicity; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D017686:Oncorhynchus mykiss; D011480:Protease Inhibitors / Q000097:blood*; D011529:Protozoan Infections, Animal; D013045:Species Specificity; D014337:Trout; D000511:alpha-Macroglobulins
chemical_list: D011480:Protease Inhibitors; D000511:alpha-Macroglobulins; D008666:Metalloendopeptidases