Simple spinner bottle with rotating basket packed with carriers for hybridoma cell culture.
r-69B is a mouse-mouse hybridoma cell line, producing monoclonal antibody IgG against human r-IFN. It was cultured for 21 days in the 1.0-L spinner bottle which was assembled with a rotating basket packed with the 8.0-g Fibra-Cel carriers. The agitation was 100 r/min. The results showed that 53.5% of the cells can be trapped within the carriers in the basket and the cell concentration and MAb was about double those in the suspension culture. The spinner bottle could be assembled simply and used in general laboratories. It also could be used for different kinds of cells, including anchorage-dependent and independent cells.
Chen
Y
Y
Laboratory of Genetic Engineering, Fujian Medical College, Fuzhou, China.
Wang
G
G
Zhang
W
W
Freedman
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002455:Cell Division; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006825:Hybridomas; D007371:Interferon-gamma / Q000276:immunology; D051379:Mice; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007371:Interferon-gamma; D005947:Glucose