Identification of vegetative proteins for a two-dimensional protein index of Bacillus subtilis.
Twenty-three of the most prominent spots which are visible on two-dimensional (2-D) protein gels of Bacillus subtilis crude extracts were selected as marker spots for the construction of a 2-D protein index. N-terminal sequencing of the corresponding proteins resulted in the identification of enzymes involved in glycolysis, TCA cycle, pentose phosphate cycle, amino acid metabolism, nucleotide biosynthesis and translation. Using computer analysis of the 2-D protein gels, most of these metabolic enzymes were found to be synthesized at a reduced rate after different stresses and glucose starvation. Such an approach permits a rapid and global evaluation of the regulation of different branches of metabolism in response to various physiological conditions.
Schmid
Roland
R
Universität Osnabrük, Abteilung für Mikrobiologie, 49076 Osnabrück, Germany.
Bernhardt
Jörg
J
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität Greifswald, 17487 Greifswald, Friedrich-Ludwig-Jahn-StraBe 15, Germany.
Antelmann
Haike
H
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität Greifswald, 17487 Greifswald, Friedrich-Ludwig-Jahn-StraBe 15, Germany.
Völker
Andrea
A
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität Greifswald, 17487 Greifswald, Friedrich-Ludwig-Jahn-StraBe 15, Germany.
Mach
Hiltraut
H
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität Greifswald, 17487 Greifswald, Friedrich-Ludwig-Jahn-StraBe 15, Germany.
Völker
Uwe
U
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität Greifswald, 17487 Greifswald, Friedrich-Ludwig-Jahn-StraBe 15, Germany.
Hecker
Michael
M
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität Greifswald, 17487 Greifswald, Friedrich-Ludwig-Jahn-StraBe 15, Germany.
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data
D001426:Bacterial Proteins