Thy-3, a developmentally regulated T-cell glycoprotein associated to Thy-1 in detergent-resistant membrane microdomains.
The function of the Thy-1 molecule, a major T-cell glycoprotein, is still obscure. Its functional properties might be due to the anchoring via a glycosylphosphatidylinositol group which favors gathering of molecules in functional membrane subregions called microdomains. Using novel monoclonal antibodies, we describe a 53-kDa Thy-1-associated glycoprotein called Thy-3. Thy-3 expression is restricted to T lymphocytes, becomes detectable on double-positive thymocytes, and depends on that of Thy-1. Anti-Thy-3 antibodies immunoprecipitate Thy-1 and Thy-3 or Thy-3 alone in detergents which preserve or disrupt microdomains, respectively. These antibodies induce thymocyte aggregation and interfere with adhesion of thymocytes to a thymic epithelial cell line as previously shown with anti-Thy-1 antibodies. Thus, Thy-3 is a T lineage-specific glycoprotein associated to Thy-1 in membrane microdomains and might contribute to the function of Thy-1 in T-cell differentiation.
Aurrand-Lions
M
M
Centre d'Immunologie, CIML INSERM-CNRS, Marseille, 13288, France.
Pierres
M
M
Naquet
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002448:Cell Adhesion / Q000276:immunology; D002449:Cell Aggregation / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D003902:Detergents / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding / Q000276:immunology; D017434:Protein Structure, Tertiary; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D018800:Thy-1 Antigens / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D003902:Detergents; D008562:Membrane Glycoproteins; D018800:Thy-1 Antigens