Transiently decreased sialylation of thyrotropin (TSH) in a patient with the euthyroid sick syndrome.
A 60-year old woman was admitted to the medical intensive care unit with respiratory distress and worsening renal function, and was found to have bilateral renal artery occlusion. Aggressive nutrition per nasogastric tube was begun on day 8 of her illness, and she eventually recovered after bilateral renal artery bypass surgery, which was performed on day 15. She developed the euthyroid sick syndrome. Levels of serum TSH, T3, and T4 fell during the first few days of her illness, then all trended to normal levels by day 28. The TSH level declined from 1.6 microU/mL on day 2 to 0.2 microU/mL on day 5, then rose to 4.5 microU/mL on day 10, and was 3.8 microU/mL on day 14. The ratios of free T4/TSH, a crude measure of the bioactivity of TSH, were 1.4, 8.0, 0.16, 0.32, and 1.14 on days 2, 5, 10, 14 and 28, respectively. TSH was immunoaffinity concentrated from serum collected on four dates. The TSH oligosaccharides were enzymatically released, treated with or without neuraminidase, labeled with a fluorescent probe, and analyzed by gel electrophoresis. The TSH oligosaccharides were found to be transiently less sialylated on day 13 as compared to days 2, 4, and 24. Three gel bands representing poorly sialylated oligosaccharides represented a mean of 20.6% of TSH oligosaccharides on days 2, 4, and 24, but represented 33.7% of TSH oligosaccharides on day 13. This is the first report of altered TSH oligosaccharide sialylation in the euthyroid sick syndrome. If confirmed by studies of additional patients, altered TSH sialylation may, in part, explain the altered TSH bioactivity that has been described in the euthyroid sick syndrome.
Magner
J
J
East Carolina University School of Medicine, Greenville, North Carolina 27858, USA.
Roy
P
P
Fainter
L
L
Barnard
V
V
Fletcher
P
P
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005067:Euthyroid Sick Syndromes / Q000188:drug therapy / Q000378:metabolism* / Q000652:urine; D005260:Female; D005456:Fluorescent Dyes; D006026:Glycoside Hydrolases / Q000096:biosynthesis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008875:Middle Aged; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism*; D013960:Thyroid Function Tests; D013972:Thyrotropin / Q000737:chemistry / Q000378:metabolism*
D005456:Fluorescent Dyes; D009844:Oligosaccharides; D012794:Sialic Acids; D013972:Thyrotropin; D006026:Glycoside Hydrolases; C020668:glycanase