Methanol-precipitated, detergent-treated Newcastle disease virus as an agar-gel precipitation test antigen.
Methanol-precipitated, detergent-treated (Triton X-100) Newcastle disease virus (NDV) was found suitable as an agar gel precipitation test (AGPT) antigen. One hundred and twenty sera were tested by the haemagglutination-inhibition (HI) test and AGPT against NDV. There was a very significant increase in the proportion of AGPT positive samples with increase in HI titre. Hence AGPT can be recommended as a field based test for seromonitoring following vaccinations against ND where laboratory facilities are inadequate.
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Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madras, India.
Koteeswaran
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Venugopalan
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AT
D000362:Agar; D000482:Allantois / Q000737:chemistry / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002642:Chick Embryo; D003902:Detergents; D005260:Female; D006385:Hemagglutination Inhibition Tests / Q000379:methods / Q000662:veterinary; D000432:Methanol; D009522:Newcastle disease virus / Q000276:immunology*; D017830:Octoxynol; D011233:Precipitin Tests / Q000379:methods / Q000662:veterinary*
D000956:Antigens, Viral; D003902:Detergents; D000362:Agar; D017830:Octoxynol; D000432:Methanol