Simultaneous determination of purine bases, ribonucleosides and ribonucleotides by capillary electrophoresis-electrochemistry with a copper electrode.
Simultaneous determination of purine bases, ribonucleosides and ribonucleotides was achieved by coupling capillary electrophoresis (CE) with wall-jet amperometric detection. A 200 microm diameter copper disk electrode was applied at working potential, +0.65 V vs. saturated calomel electrode. The current response of high sensitivity and stability was obtained in strong basic solutions which were suitable for satisfactory CE separations. The calibration curve was linear over 2-3 orders of magnitude and the limits of detection for adenine, guanine, xanthine, uric acid, adenosine, guanosine, adenosine-5'- monophosphate and guanosine-5'-monophosphate were below 9 fmol (SIN=3). The use of this method for the separation and detection of compounds present in human plasma samples was reported.
Lin
H
H
Department of Chemistry, Nanjing University, China.
Xu
D K
DK
Chen
H Y
HY
D002138:Calibration; D003300:Copper / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D011687:Purines / Q000097:blood*; D012263:Ribonucleosides / Q000097:blood*; D012265:Ribonucleotides / Q000097:blood*; D012680:Sensitivity and Specificity
D011687:Purines; D012263:Ribonucleosides; D012265:Ribonucleotides; D003300:Copper