A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
The 465,813 base pair sequence corresponding to the 40.1-50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2-46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified a new asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the cis-element.
Itoh
T
T
Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Japan.
Aiba
H
H
Baba
T
T
Hayashi
K
K
Inada
T
T
Isono
K
K
Kasai
H
H
Kimura
S
S
Kitakawa
M
M
Kitagawa
M
M
Makino
K
K
Miki
T
T
Mizobuchi
K
K
Mori
H
H
Mori
T
T
Motomura
K
K
Nakade
S
S
Nakamura
Y
Y
Nashimoto
H
H
Nishio
Y
Y
Oshima
T
T
Saito
N
N
Sampei
G
G
Seki
Y
Y
Horiuchi
T
T
D001287:Attachment Sites, Microbiological; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005810:Multigene Family; D016366:Open Reading Frames; D012348:RNA, Transfer, Asn / Q000235:genetics; D012093:Replicon; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D012348:RNA, Transfer, Asn