Determination of pyronaridine in blood plasma by high-performance liquid chromatography for application in clinical pharmacological studies.
A method is described for the determination of pyronaridine in plasma using high-performance liquid chromatography with fluorescence detection. The method involves liquid-liquid extraction with phosphate buffer (pH 6.0, 0.05 M) and diethyl ether-hexane (70:30%, v/v) and chromatographic separation on a C18 column (Nucleosil, 250 x 4.6 mm I.D., 5 microns particle size) with acetonitrile-0.05 M phosphate buffer pH 6.0 (60:40%, v/v) as the mobile phase (1 ml/min) and detection by fluorescence (lambda ex = 267 nm, lambda em = 443 nm). The detector response is linear up to 1000 ng and the overall recoveries of pyronaridine and quinine were 90.0 and 60.3%, respectively. The assay procedure was adequately sensitive to measure 10 ng/ml pyronaridine in plasma samples with acceptable precision (< 15% C.V.). The method was found to be suitable for use in clinical pharmacological studies.
Jayaraman
S D
SD
Centre for Drug Research, Universiti Sains Malaysia, Penang, Malaysia.
Ismail
S
S
Nair
N K
NK
Navaratnam
V
V
D000962:Antimalarials / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D009287:Naphthyridines / Q000097:blood* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence
D000962:Antimalarials; D009287:Naphthyridines; C027871:pyronaridine