The effects of cytomegalovirus infection on polar lipids and neutral lipids in cultured human cells.
The effects of infection by the human cytomegaloviruses Ad-169 on the incorporation of [14C]acetate into the polar and neutral lipids of human embryonic lung cells and human saphenous vein smooth muscle cells were compared to [14C]acetate incorporation in mock-infected control cells. Cytomegalovirus infection caused a shift in the relative amounts of polar and neutral lipids, with infected cells having lower amounts of polar lipids and higher amounts of neutral lipids than mock-infected controls. When neutral lipids were separated into diglyceride (DG), cholesterol (C), fatty acid, triglyceride (TG) and cholesterol ester (CE) components, Ad-169-infected cells had lower levels of incorporation of label into CE, TG, and DG fractions, and higher levels of label incorporation into C than mock-infected cells.
Abrahamsen
L H
LH
Bates College, Department of Biology, Lewiston, ME 04240, USA.
Clay
M J
MJ
Lyle
J M
JM
Zink
J M
JM
Fredrikson
L J
LJ
DeSiervo
A J
AJ
Jerkofsky
M A
MA
D000085:Acetates / Q000378:metabolism; D002460:Cell Line; D003587:Cytomegalovirus / Q000502:physiology*; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D012501:Saphenous Vein / Q000166:cytology
D000085:Acetates; D008055:Lipids