Enhanced expression of HLA-DR, Fc gamma receptor 1 (CD64) and leukocyte common antigen (CD45) indicate activation of monocytes in regenerative training periods of endurance athletes.
The aim of this study was to test the hypothesis whether endurance athletes have distinct expressions of functionally relevant surface antigens of monocytes. Under standardized experimental and laboratory conditions CD 14+ blood monocytes of 55 males (22 sedentary controls [CO], individual anaerobic threshold [IAT]: 2.2 +/- 1.2 W.kg-1, VO2 max: 47 +/- 12 ml.min-1.kg-1; 13 endurance athletes, training: 3-10 hours weekly [E1], IAT: 3.0 +/- 1.1 W.kg-1, VO2max 57 +/- 5 ml.min-1.kg-1; 20 endurance athletes training: > 10 hours weekly [E2], IAT: 3.7 +/- 1.3 W.kg-1, VO2max: 64 +/- 6 ml.min-1.kg-1) were investigated for relative surface receptor expression of CD45, CD64 and HLA-DR (direct immunofluorescence, flow cytometry). E1 and E2 showed significantly (ANOVA, Tukey's HSD test; each p < 0.01) higher relative receptor expressions for CD64 in comparison to CO (E1: +62/E2: +71%; percentage of increase compared to CO value), CD45 (+44/+32%) and HLA-DR (+22/+35%). Cell counts of four monocyte subpopulations (CD56+, CD14bright+CD16-, CD14bright+CD16dim+, CD14dim(+)-CD16bright+) did not show significant differences between the groups. These data suggest that in endurance athletes during regenerative training period circulating monocytes show an enhancement of functionally relevant surface receptors indicating an activation monocytes.
Gabriel
H
H
Institute of Sports and Performance Medicine, University of the Saarland, Saarbrücken, Germany.
Rothe
G
G
Korpys
M
M
Schmitz
G
G
Kindermann
W
W
D000328:Adult; D015308:Anaerobic Threshold; D000704:Analysis of Variance; D001642:Bicycling / Q000502:physiology; D019002:CD56 Antigen / Q000032:analysis / Q000235:genetics; D005434:Flow Cytometry; D019085:Fluorescent Antibody Technique, Direct; D005786:Gene Expression Regulation; D006684:HLA-DR Antigens / Q000032:analysis* / Q000235:genetics; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis* / Q000235:genetics; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000235:genetics; D008297:Male; D009000:Monocytes / Q000276:immunology*; D010101:Oxygen Consumption; D010806:Physical Education and Training; D010807:Physical Endurance / Q000276:immunology*; D017452:Receptors, IgG / Q000032:analysis* / Q000235:genetics; D012038:Regeneration / Q000276:immunology*; D012420:Running / Q000502:physiology; D013177:Sports / Q000502:physiology*; D013550:Swimming / Q000502:physiology
D019002:CD56 Antigen; D006684:HLA-DR Antigens; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D017493:Leukocyte Common Antigens