Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis.
The Saccharomyces cerevisiae BNI1 gene product (Bni1p) is a member of the formin family of proteins, which participate in cell polarization, cytokinesis, and vertebrate limb formation. During mating pheromone response, bni1 mutants showed defects both in polarized morphogenesis and in reorganization of the underlying actin cytoskeleton. In two-hybrid experiments, Bni1p formed complexes with the activated form of the Rho-related guanosine triphosphatase Cdc42p, with actin, and with two actin-associated proteins, profilin and Bud6p (Aip3p). Both Bni1p and Bud6p (like Cdc42p and actin) localized to the tips of mating projections. Bni1p may function as a Cdc42p target that links the pheromone response pathway to the actin cytoskeleton.
Evangelista
M
M
Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
Blundell
K
K
Longtine
M S
MS
Chow
C J
CJ
Adames
N
N
Pringle
J R
JR
Peter
M
M
Boone
C
C
D000199:Actins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D003285:Contractile Proteins; D003599:Cytoskeleton / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism; D009024:Morphogenesis; D016296:Mutagenesis; D051304:Profilins; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
D000199:Actins; C105191:Bni1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D003285:Contractile Proteins; D005656:Fungal Proteins; D008840:Microfilament Proteins; D051304:Profilins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D019204:GTP-Binding Proteins; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae