Analysis of selenium in bovine liver by gas chromatography with mass-selective, electron-capture and nitrogen-phosphorus detection.
The concentration of selenium (Se) in liver was determined by gas chromatography (GC) with mass-selective (GC-MS), electron capture (GC-ECD) and nitrogen-phosphorus (GC-NPD) detection. Liver samples were digested in a mixture containing HNO3 and Mg(NO3). SeVI was converted to SeIV. SeIV was derivatized with 4-nitrophenylenediamine and then extracted in toluene. A 1-microliter volume of the toluene extract was analyzed by the GC-MS, GC-ECD or GC-NPD methods. The detection limits of the GC-ECD, the GC-NPD and the GC-MS methods were 25, 50 and 800 pg, respectively. The GC-NPD method was more selective for the derivatized Se than the GC-ECD method. The GC-MS method had the advantage of using the 76Se isotope as the internal standard. Se concentrations in liver samples determined by the three methods were comparable.
Singh
A K
AK
Minnesota Veterinary Diagnostic Laboratory, Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA.
White
T
T
Arendt
T
T
Jiang
Y
Y
D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry*; D012643:Selenium / Q000032:analysis*
D012643:Selenium