title: Timing of c-jun protein induction in lumbar dorsal root ganglia after sciatic nerve transection varies with lesion distance.
abstract: In situ hybridization and immunohistochemical studies have shown that sciatic nerve crush or transection induces upregulation of the immediate early gene c-jun mRNA and protein in lumbar dorsal root ganglion neurons. Here we have used enhanced chemiluminescent (ECL) immunoblotting as a sensitive and quantitative way of measuring the time of course of c-jun protein induction following sciatic nerve transection at two distances from the L4 and L5 dorsal root ganglia. c-Jun protein was first detected within 3 h of proximal sciatic nerve transection and within 6 h of distal nerve transection. These results indicate substantially earlier increases in c-jun protein after nerve injury than previously reported, which can be attributed to the sensitivity of this detection method. The earlier induction of c-jun after proximal as compared to distal nerve transection supports the hypothesis that the c-jun response to sciatic nerve injury involves a distance-dependent signalling mechanism.
authors.affiliation: Department of Neurology, Yale University School of Medicine, New Haven, CT 06510, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Kenney
authors.forename: J D
authors.initials: JD
authors.lastname: Kocsis
mesh_terms: D000818:Animals; D001345:Autoradiography; D003714:Denervation; D005260:Female; D005727:Ganglia, Spinal / Q000737:chemistry / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000601:surgery*; D013997:Time Factors
chemical_list: D016755:Proto-Oncogene Proteins c-jun