title: Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII.
abstract: A 9.9 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains four open reading frames (ORFs) longer than 100 amino acids. One gene, PFK1, has already been cloned and sequenced and the other one is the probable yeast gene coding for the beta-subunit of the succinyl-CoA synthetase. The two remaining ORFs share homology with the deduced amino acid sequence (and their physical arrangement is similar to that) of the YHR161c and YHR162w ORFs from chromosome VIII.
authors.affiliation: Laboratório de Genética Molecular, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Guerreiro
authors.forename: D
authors.initials: D
authors.lastname: Azevedo
authors.forename: T
authors.initials: T
authors.lastname: Barreiros
authors.forename: C
authors.initials: C
authors.lastname: Rodrigues-Pousada
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis*; D016681:Genome, Fungal; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013389:Succinate-CoA Ligases / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; C048633:Ptms protein, rat; D013389:Succinate-CoA Ligases