title: IgE epitope analysis of the hevein preprotein; a major latex allergen.
abstract: We have previously identified the hevein preprotein as a common allergen for latex allergic healthcare workers. The B cell epitopes in the hevein protein that are recognized by IgE of latex-allergic individuals have not been identified. In this study, we examined the hevein preprotein using epitope mapping. Overlapping synthetic peptides of 10 amino acids (two aa overlap) were synthesized on a derivatized cellulose membrane using Fmoc chemistry. The peptide spots were probed with pooled sera from 10 latex-allergic patients, and the IgE-reactive peptides identified with anti-IgE MoAbs. We identified six B cell epitopes within the full length hevein preprotein which bound IgE from latex-allergic patients. Two were located in the N-terminal 5-kD hevein domain and four were observed in the 14-kD C-domain. A broad epitope was located between the N-terminal amino acids 13-24. This epitope had nearly complete homology to wheat germ agglutinin (WGA). Immunological cross-reactivity to WGA was confirmed by Western blot analysis with purified WGA, and this reactivity could be inhibited by latex proteins or WGA. Of the five remaining epitopes, four had homologies to other proteins in the pathogenesis-related family of plant proteins (PR-4). The data demonstrate that hevein has multiple IgE epitopes. The significant homology of these epitopes to a broad family of plant defence proteins further explains the increased prevalence of food allergies in latex-allergic individuals.
authors.affiliation: Laboratory of Macrophage Biology, Guthrie Research Institute, Sayre, PA 18840, USA.
authors.forename: D H
authors.initials: DH
authors.lastname: Beezhold
authors.forename: D A
authors.initials: DA
authors.lastname: Kostyal
authors.forename: G L
authors.initials: GL
authors.lastname: Sussman
mesh_terms: D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology*; D007840:Latex / Q000276:immunology; D037102:Lectins / Q000276:immunology*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010940:Plant Proteins / Q000276:immunology*; D011498:Protein Precursors / Q000276:immunology*; D014909:Wheat Germ Agglutinins / Q000276:immunology
chemical_list: D000485:Allergens; D023181:Antimicrobial Cationic Peptides; D018985:Epitopes, B-Lymphocyte; D007840:Latex; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D011498:Protein Precursors; D014909:Wheat Germ Agglutinins; C045875:hevein; D007073:Immunoglobulin E