Assay for the (R)- and (S)-enantiomers of salsolinols in biological samples and foods with ion-pair high-performance liquid chromatography using beta-cyclodextrin as a chiral mobile phase additive.
A chromatographic procedure was devised for the quantitative determination of the enantiomers of salsolinol and N-methylsalsolinol, which are biologically important alkaloids. The enantiomers of salsolinol and N-methylsalsolinol were completely separated using beta-cyclodextrin in a reversed-phase ion-pair system. The HPLC method was sensitive enough to detect the isoquinolines at a concentration less than 0.1 pmol per injection. The presence of (R)- and (S)-salsolinol was confirmed in fermented foods and beverages, while N-methylsalsolinol was not detected. On the other hand, the (R)-enantiomers of both salsolinol and N-methylsalsolinol were found to predominate in the human brain.
Deng
Y
Y
Department of Applied Chemistry, Nagoya Institute of Technology, Japan.
Maruyama
W
W
Kawai
M
M
Dostert
P
P
Yamamura
H
H
Takahashi
T
T
Naoi
M
M
D001628:Beverages / Q000032:analysis; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry*; D005504:Food Analysis; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007546:Isoquinolines / Q000032:analysis*; D008968:Molecular Conformation; D015203:Reproducibility of Results; D012490:Salsoline Alkaloids / Q000032:analysis*; D044005:Tetrahydroisoquinolines; D014920:Wine / Q000032:analysis; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D007546:Isoquinolines; D012490:Salsoline Alkaloids; D044005:Tetrahydroisoquinolines; D047392:beta-Cyclodextrins; C036617:salsolinol; C032837:salsoline; C031215:betadex