title: Nucleotide sequence of an adult-specific cuticular protein gene from the beetle Tenebrio molitor: effects of 20-hydroxyecdysone on mRNA accumulation.
abstract: The accumulation of transcripts from two adult-specific cuticular genes (ACP-20 and ACP-22) is shown to be modified after addition of exogenous 20-hydroxyecdysone. In the continuous presence of high levels of the hormone, the expression of ACP-20 gene is significantly weaker than that of untreated controls, while ACP-22 expression is 2.5-fold increased. During active synthesis of the ACP messages, a 0.5 microg 20-hydroxyecdysone injection causes a rapid 2-fold increase in ACP-22 mRNA and is not able to repress ACP-20 mRNA accumulation. We conclude that these genes whose transcripts appear in an almost coordinated manner in epidermal cells during the moulting cycle are regulated by ecdysteroids in a different way. In order to undertake a functional dissection of the promoter regions of ACP-22 gene, we have isolated and sequenced a genomic clone. The sequence similarities with other cuticular protein genes are discussed.
authors.affiliation: URA CNRS 674, Developpement, Communication Chimique, Universite de Bourgogne, Dijon, France.
authors.forename: H
authors.initials: H
authors.lastname: Bouhin
authors.forename: C
authors.initials: C
authors.lastname: Braquart
authors.forename: J P
authors.initials: JP
authors.lastname: Charles
authors.forename: B
authors.initials: B
authors.lastname: Quennedey
authors.forename: J
authors.initials: J
authors.lastname: Delachambre
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D004441:Ecdysterone / Q000494:pharmacology*; D005786:Gene Expression Regulation; D017344:Genes, Insect; D016678:Genome; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D013712:Tenebrio / Q000235:genetics* / Q000378:metabolism
chemical_list: C105435:ACP-22 protein, Tenebrio molitor; D019476:Insect Proteins; D012333:RNA, Messenger; C047663:cuticle proteins, insects; C075200:ACP-20 protein, Tenebrio molitor; D004441:Ecdysterone; D004247:DNA