Control of the Myc-Max mediated transactivation in yeast by natural promoter elements.
Transcriptional activation studies involving the human oncoprotein and transcription factor Myc and its helix-loop-helix partner protein Max in mammalian cells are critical due to the presence of endogenous Myc and Max proteins. Here we show that co-expression of the human c-myc and max genes from 2micro circle derived high copy number vectors in yeast cells stimulate the transcriptional activation of a LacZ reporter gene fused to the yeast cytochrome-c1 oxidase minimal promoter containing the adenovirus major late promoter element (AMLPE). The exchange of the single Myc binding site in the AMLPE by the two E-box DNA motifs (CACGTG) present in the Myc responsive element of a human Myc target gene (ornithine decarboxylase) in front of a promoter-reporter gene cassette results in a two-fold enhanced beta-galactosidase expression. Low expression of max and high level expression of c-myc at the same time led to a further enhancement of transcriptional activation from this promoter-reporter gene cassette.
Hänel
F
F
Hans-Knöll-Institut für Naturstoff-Forschung e.V., Jena, Germany.
Peukert
K
K
Munder
T
T
D000256:Adenoviridae / Q000235:genetics; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D050976:Basic-Leucine Zipper Transcription Factors; D015153:Blotting, Western; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D016259:Genes, myc; D005822:Genetic Vectors; D006801:Humans; D007763:Lac Operon; D009955:Ornithine Decarboxylase / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors; D015533:Transcriptional Activation; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; C499330:MAX protein, human; C067850:Myc associated factor X; D014157:Transcription Factors; D003576:Electron Transport Complex IV; D001616:beta-Galactosidase; D009955:Ornithine Decarboxylase