title: Recall response to cytomegalovirus in allograft recipients: mobilization of CD57+, CD28+ cells before expansion of CD57+, CD28- cells within the CD8+ T lymphocyte compartment.
abstract: BACKGROUND
  Strong correlations have been described between persistently elevated proportions of CD57+ (CD28-) CD8+high T lymphocytes and cytomegalovirus (CMV) infection, in healthy individuals as well as in transplant patients. We investigated whether secondary exposure to CMV triggers recall responses within the CD8 T cell compartment.
  
  
  METHODS
  In a longitudinal study in 123 kidney recipients, we compared 17 primary CMV infections with 27 secondary CMV infections. Subset composition of the CD8 compartment was analyzed by flow cytometry.
  
  
  RESULTS
  CD8 lymphocytosis occurred significantly earlier (by 17 days on average) in CMV reactivations than in primary infections. Both in primary and secondary infections, CD28+ CD8+high T lymphocytes were mainly recruited at the start. In formerly CMV-seropositive patients, preexisting CD57+ CD8+high T lymphocytes switched at the start from no expression of CD28 to high expression of CD28 and, concomitantly, from CD45RA to high expression of CD45RO. These cells reverted rapidly to a CD28- and CD45RA+ phenotype. Nevertheless, the accumulation of CD57+ (CD28-) CD8+high T cells was delayed similarly in primary and secondary CMV infection, progressing over a period between 2 and 8 weeks after the onset of CD8 lymphocytosis to plateau at 366 CD57+ CD8+high cells/ mm3 on average.
  
  
  CONCLUSIONS
  The faster kinetics of CD8 lymphocytosis in secondary CMV infection suggests that a recall response triggers cycling "memory" cells within the CD28+ CD8+high subset, while preexistent CD57+ CD8+high T cells with a long-lived cell phenotype can also be mobilized, possibly through the transient acquisition of CD28 expression. The protracted accumulation of CD57+ (and CD28-) lymphocytes might then reflect an end-stage differentiation.
authors.affiliation: Service d'Immunologie, Faculte de Médecine et CHRU de Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Hazzan
authors.forename: M
authors.initials: M
authors.lastname: Labalette
authors.forename: C
authors.initials: C
authors.lastname: Noel
authors.forename: G
authors.initials: G
authors.lastname: Lelievre
authors.forename: J P
authors.initials: JP
authors.lastname: Dessaint
mesh_terms: D018106:CD28 Antigens / Q000276:immunology*; D018959:CD57 Antigens / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory; D016030:Kidney Transplantation / Q000276:immunology*; D007700:Kinetics; D008137:Longitudinal Studies; D010641:Phenotype
chemical_list: D018106:CD28 Antigens; D018959:CD57 Antigens; D016827:CD8 Antigens