Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex.
Previous studies suggested the existence of V1a vasopressin receptors (V1aR) in the cerebral cortex. Here, we investigated the cellular and regional localization of V1aR in the E18 rat cerebral cortex using RT-PCR and Southern blot analysis of V1aR mRNA derived from enriched cultures of neurons, astroglia, and oligodendroglia from four cortical regions (rostral, caudal, dorsal and ventral). V1aR mRNA was detected in each of the cell types within each of the regions studied. These data indicate that V1aR is broadly distributed throughout the cerebral cortex and suggest that vasopressin plays an important role in cortical functions.
Yamazaki
R S
RS
Department of Molecular Pharmacology and Toxicology, University of Southern California Pharmaceutical Sciences Center, Los Angeles 90033, USA.
Chen
Q
Q
Schreiber
S S
SS
Brinton
R D
RD
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D017931:DNA Primers; D004622:Embryo, Mammalian; D009474:Neurons / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism*; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017483:Receptors, Vasopressin / Q000096:biosynthesis*; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D017483:Receptors, Vasopressin