[Influence of visible interferences on biochemical assays realised on Au 5231, AU 5223 (Olympus) and CL 7200 (Shimadzu)].
The impact of the major interferents (hemolysis, bilirubin, turbidity), on the quality of biochemical tests, was evaluated on multiparametric analysers (CL 7200 Shimadzu, Japan/Ciba-Corning, France; AU 5231 and AU 5223 Olympus, Japan/bioMérieux, France), according to the SFBC instructions. Interferences were detected in 33 cases upon 165 tests realized, that is to say 20% of the performed analysis. Turbidity was the most frequent cause of interference (7.8%), followed by hemolysis (8.5%) and bilirubin (3.6%). The use of a sample blank, a bireagent, the change of reagent, the change of the secondary wavelength or the modification of the measurement times, allowed us to reduce more than 80% of the interferences. Only three interferences remained: hemolysis upon the measurement of TGO and potassium, and bilirubin upon the measurement of creatinine. For these parameters, a suitable note using the Olympus factors (semi quantitative expression of the importance of the three interferents) is reported on the answer sheet.
Frémont
S
S
Laboratoire de biochimie automatisée, CHU de Nancy-Brabois, Vandaeuvre-lès-Nancy, France.
Combe
A M
AM
Mecrin
M
M
Tronel
H
H
Galland
A V
AV
Scholl
C
C
Nicolas
J P
JP
D000818:Animals; D001663:Bilirubin / Q000494:pharmacology; D001774:Blood Chemical Analysis / Q000379:methods*; D004347:Drug Interactions; D006461:Hemolysis; D009391:Nephelometry and Turbidimetry
D001663:Bilirubin
Influence des interférences visibles sur les dosages de biochimie réalisés sur AU 5231, AU 5223 (Olympus) et CL 7200 (Shimadzu).