Structure of the O-specific polysaccharide of Proteus penneri strain 25 containing N-(L-alanyl) and multiple O-acetyl groups in a tetrasaccharide repeating unit.
Based on sugar and methylation analyses, O-deacetylation, Smith degradation, and 1H and 13C NMR spectroscopy, including 2D COSY, 1H-detected 1H, 13C heteronuclear single-quantum coherence (HSQC), and 1H-detected 1H, 13C heteronuclear multiple-bond connectivity (HMBC) experiments, the following structure of the O-specific polysaccharide of Proteus penneri strain 25 was established: [formula: see text] where D-GlcN(L-Ala) is 2-(L-alanylamido)-2-deoxy-D-glucose.
Arbatsky
N P
NP
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Shashkov
A S
AS
Widmalm
G
G
Knirel
Y A
YA
Zych
K
K
Sidorczyk
Z
Z
D000107:Acetylation; D000409:Alanine / Q000737:chemistry; D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011511:Proteus / Q000737:chemistry* / Q000145:classification; D016339:Spectrometry, Mass, Fast Atom Bombardment
D019081:O Antigens; D009844:Oligosaccharides; D000409:Alanine