Characterization of polymorphisms in the toxin A and B genes of Clostridium difficile.
We have used six independent polymerase chain reactions (A1-A3 and B1-B3) for amplification of the entire sequence of the two toxin genes tcdA and tcdB of several Clostridium difficile strains. With this approach we have detected (1) restriction site polymorphisms which are distributed all over the genes, and (2) deletions that could be found only in tcdA. Characteristic differences between strains were mainly focused to the 5' third of tcdB (B1 fragment) and/or the 3' third of tcdA (A3 fragment). The possible use of our approach for typing of C. difficile toxin genes is discussed.
Rupnik
M
M
Verfugungsgebäude für Forschung und Entwicklung, Johannes Gutenberg-Universität Mainz, Germany.
Braun
V
V
Soehn
F
F
Janc
M
M
Hofstetter
M
M
Laufenberg-Feldmann
R
R
von Eichel-Streiber
C
C
D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000235:genetics*; D016360:Clostridioides difficile / Q000235:genetics*; D004768:Enterotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile