Primary structure and strand specificity of BstF5I-1 DNA methyltransferase which recognizes 5'-GGATG-3'.
The gene for BstF5I-1 DNA-methyltransferase (MTase) from Bacillus stearothermophilus F5 (a FokI isoschizomer, recognizing 5'-GGATG-3') was cloned and its nucleotide (nt) sequence was determined. Analysis of deduced amino acid (aa) sequence shows that M x BstF5I-1 belongs to D21 class of MTases and has a little homology with M x FokI. M x BstF5I-1 modifies only the upper strand of recognition sequence (5'-GGATG-3').
Degtyarev
S Kh
SKh
SibEnzyme, Novosibirsk, Russia.
Netesova
N A
NA
Abdurashitov
M A
MA
Shevchenko
A V
AV
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D019175:DNA Methylation; D004269:DNA, Bacterial / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics* / Q000378:metabolism; D013379:Substrate Specificity
D004269:DNA, Bacterial; C058668:DNA modification methylase FokI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)