title: The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii x Aloe aristata (Aloaceae), to its progeny.
abstract: Genomic in situ hybridization (GISH) was used to study somatic chromosomes of parental and progeny plants (all 2n=2x=14) of the bigeneric hybrid between Gasteria lutzii and Aloe aristata (Aloaceae), which is partially fertile, a rare occurrence in plants. GISH successfully distinguished between the two parental genomes in the F1 hybrid and revealed numerous genomic recombinations in chromosomes transmitted by the F1 to the back-cross progeny. The results indicate high levels of meiotic compatibility between the parental genomes, even though they differ in size by 20%. Recombination occurred at a frequency that was higher than that expected from the analysis of orcein-stained meiosis in the F1. The discrepancy suggests that terminalization may occur prior to or during metaphase I, reducing the apparent chiasma frequency, or possibly reveals an under-estimation caused by difficulties in resolving closely grouped chiasmata by eye.
authors.affiliation: Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK.
authors.forename: C
authors.initials: C
authors.lastname: Takahashi
authors.forename: I J
authors.initials: IJ
authors.lastname: Leitch
authors.forename: A
authors.initials: A
authors.lastname: Ryan
authors.forename: M D
authors.initials: MD
authors.lastname: Bennett
authors.forename: P E
authors.initials: PE
authors.lastname: Brandham
mesh_terms: D000504:Aloe / Q000235:genetics; D002678:Chimera / Q000235:genetics*; D002875:Chromosomes; D003433:Crosses, Genetic; D005787:Gene Frequency; D017403:In Situ Hybridization / Q000379:methods*; D008540:Meiosis; D008677:Metaphase; D010078:Oxazines / Q000737:chemistry; D010944:Plants / Q000235:genetics*; D010946:Plants, Medicinal; D011995:Recombination, Genetic; D013194:Staining and Labeling
chemical_list: D010078:Oxazines; C002313:PAcein