title: A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody.
abstract: Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10(-7) to 10(-9) M.
authors.affiliation: Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Kobatake
authors.forename: H
authors.initials: H
authors.lastname: Sasakura
authors.forename: T
authors.initials: T
authors.lastname: Haruyama
authors.forename: M L
authors.initials: ML
authors.lastname: Laukkanen
authors.forename: K
authors.initials: K
authors.lastname: Keinänen
authors.forename: M
authors.initials: M
authors.lastname: Aizawa
mesh_terms: D001667:Binding, Competitive; D005452:Fluoresceins; D005456:Fluorescent Dyes; D015200:Fluoroimmunoassay; D005818:Genetic Engineering; D006241:Haptens; D008081:Liposomes / Q000276:immunology*; D010081:Oxazolone / Q000031:analogs & derivatives
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D006241:Haptens; D008081:Liposomes; C018361:2-phenyloxazolone; D010081:Oxazolone; C024098:6-carboxyfluorescein