The p120-v-Abl protein interacts with E2F-1 and regulates E2F-1 transcriptional activity.
The E2F family of transcription factors regulates cell cycle progression, and deregulated expression of E2F-1 can lead to neoplastic transformation. In myeloid cells, introduction and expression of the Abelson leukemia virus causes growth factor independence. Here, the p120 v-Abl protein activates E2F-1-mediated transcription through a physical interaction with the E2F-1 transcription factor. BCR-Abl and c-Abl also stimulate E2F-1-mediated transcription. Our results suggest a new mechanism by which v-Abl leads to factor-independent myeloid cell proliferation: the activation of E2F-1-mediated transcription.
Birchenall-Roberts
M C
MC
Intramural Research Support Program, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201, USA.
Yoo
Y D
YD
Bertolette
D C
DC
Lee
K H
KH
Turley
J M
JM
Bang
O S
OS
Ruscetti
F W
FW
Kim
S J
SJ
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002455:Cell Division; D004268:DNA-Binding Proteins / Q000378:metabolism*; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D005656:Fungal Proteins / Q000378:metabolism; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism; D051379:Mice; D016374:Oncogene Proteins v-abl / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism; D056465:Retinoblastoma-Binding Protein 1; D029701:Saccharomyces cerevisiae Proteins; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
C533431:Arid4a protein, mouse; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; C493754:E2f1 protein, mouse; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D016374:Oncogene Proteins v-abl; D056465:Retinoblastoma-Binding Protein 1; D029701:Saccharomyces cerevisiae Proteins; D050680:Transcription Factor DP1; D014157:Transcription Factors; D016044:Fusion Proteins, bcr-abl; D016315:Proto-Oncogene Proteins c-abl