A single cDNA encodes all three Aedes leucokinins, which stimulate both fluid secretion by the malpighian tubules and hindgut contractions.
A cDNA encoding preproleucokinin was isolated from a cDNA library of the mosquito Aedes aegypti. The deduced amino acid sequence of Aedes preproleucokinin contains a putative signal peptide of 18 amino acid residues and a 210-amino acid residue proleucokinin. Within the proleucokinin are encoded one copy each of the Aedes leucokinins 1, 2, and 3 isolated previously from this species (Veenstra, J. A. (1994) Biochem. Biophys. Res. Commun. 202, 715-719). All three Aedes leucokinins depolarize the transepithelial voltage of the malpighian tubule in concentrations of less than 10(-9) M and increase the frequency of hindgut contractions at concentrations above 10(-8) M. At higher concentrations the Aedes leucokinins 1 and 3 but not Aedes leucokinin 2 are also able to increase the rate of fluid secretion by the malpighian tubules. The differences of the three Aedes leucokinins in their potencies to induce fluid secretion or depolarizations in the malpighian tubules suggest that there may be more than one type of leucokinin receptor in this tissue.
Veenstra
J A
JA
Department of Entomology and Center for Insect Science, The University of Arizona, Tucson, Arizona 85721, USA. veenstra@invertebre.u-bordeaux.fr
Pattillo
J M
JM
Petzel
D H
DH
D000330:Aedes / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D004068:Digestive System Physiological Phenomena; D004594:Electrophysiology; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005260:Female; D066298:In Vitro Techniques; D019476:Insect Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D008317:Malpighian Tubules / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis
D018076:DNA, Complementary; D019476:Insect Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins