Simultaneous determination of a novel calcium entry blocker, monatepil maleate, and its metabolites in rat plasma by means of solid-phase extraction and reversed-phase liquid chromatography with electrochemical detection.
A reversed-phase LC method with electrochemical detection is described for the simultaneous determination of monatepil maleate (AJ-2615, AJ), a novel calcium entry blocker, and its three S-oxidized metabolites in plasma. These compounds were extracted from plasma by solid-phase extraction and injected onto an ODS column. The determination limit in plasma (0.5 ml) was 10 ng/ml for AJ and 5 ng/ml for the three metabolites. The method was applied to the determination of AJ and the metabolites in rat plasma samples.
Kurono
M
M
Discovery research Laboratories I, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
Suzuki
K
K
Yoshida
K
K
Naruto
S
S
D000818:Animals; D002121:Calcium Channel Blockers / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D003988:Dibenzothiepins / Q000097:blood*; D008297:Male; D015394:Molecular Structure; D010879:Piperazines / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
D002121:Calcium Channel Blockers; D003988:Dibenzothiepins; D010879:Piperazines; C067818:monatepil