[Analysis of glycosylated A1c hemoglobin by liquid phase chromatography and immunoagglutination].
Glycated hemoglobin A1c assay is performed using HPLC on cation exchange TSK SP 5 PW column. Separation is obtained in 10 minutes using graduated elution with sodium chloride and Bis-Tris buffer. Hb A1c is resolved from the other hemoglobins (F, A0 and S). Reproductibility of method is quite good and correlated with an immunologic method (DCA 2000).
Sellami
M
M
Ecole Supérieure des Sciences et Techniques de la Santé de Tunis.
Labaied
N
N
Ghanem
A
A
Nagati
K
K
Fattoum
S
S
D000372:Agglutination Tests; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006442:Glycated Hemoglobin / Q000378:metabolism*; D006453:Hemoglobinopathies / Q000097:blood*; D006801:Humans; D007159:Immunologic Tests; D015203:Reproducibility of Results; D013997:Time Factors
D006442:Glycated Hemoglobin
Dosage de l'hémoglobine glycosylée A1c par chromatographie en phase liquide et par immunoagglutination.