title: Regulation of motoneuronal calcitonin gene-related peptide (CGRP) during axonal growth and neuromuscular synaptic plasticity induced by botulinum toxin in rats.
abstract: The aim of this study was to examine whether changes in rat motoneuronal calcitonin gene-related peptide (CGRP) can be correlated with axonal growth and plasticity of neuromuscular synapses. Nerve terminal outgrowth was induced by local paralysis with botulinum toxin. Normal adult soleus and tibialis anterior did not show detectable CGRP content at the motor endplates. Following botulinum toxin injection there was a progressive, transient and bimodal increase in CGRP in both motoneuron cell bodies which innervated poisoned muscles and their motor endplates. CGRP content was moderately increased 1 day after paralysis and, after an initial decline, reached a peak 20 days after injection. This was followed by a gradual decrease and a return to normal levels at the 200th day. CGRP changes in intoxicated endplates were less evident in the tibialis anterior than in the soleus muscle. The CGRP content in motoneurons was positively correlated with the degree of intramuscular nerve sprouting found by silver staining. In situ hybridization revealed an increase in CGRP mRNA in spinal cord motoneurons 20 days after toxin administration. We conclude that motoneurons regulate their CGRP in situations in which peripheral synapse remodelling and plasticity occur.
authors.affiliation: Unitat de Neurobiologia Cellular, Departament de Cìences Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Catalonia, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Tarabal
authors.forename: J
authors.initials: J
authors.lastname: Calderó
authors.forename: J
authors.initials: J
authors.lastname: Ribera
authors.forename: A
authors.initials: A
authors.lastname: Sorribas
authors.forename: R
authors.initials: R
authors.lastname: López
authors.forename: J
authors.initials: J
authors.lastname: Molgó
authors.forename: J E
authors.initials: JE
authors.lastname: Esquerda
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D001905:Botulinum Toxins / Q000494:pharmacology*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D008297:Male; D009046:Motor Neurons / Q000187:drug effects / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000502:physiology; D009473:Neuronal Plasticity; D010243:Paralysis / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000502:physiology
chemical_list: D001905:Botulinum Toxins; D015740:Calcitonin Gene-Related Peptide