Shear stress-induced myosin association with cytoskeleton and phosphorylation in human platelets.
Upon platelet activation by a high shear stress (108 dyne/cm2), actin and actin-binding protein increased rapidly into the Triton-insoluble cytoskeleton, whereas the association of myosin increased gradually. The amounts of cytoskeleton-associated myosin depended on the extent of aggregation. Preceding the maximal aggregation and ATP secretion, the 20 kDa light chain of myosin (MLC) is rapidly phosphorylated to approx. 45% of 20 kDa MLC and is then dephosphorylated. Cytoskeletal association of myosin and phosphorylation of 20 kDa MLC was inhibited by OP-41483, a prostaglandin I2 analog, which inhibited the full aggregation response to shear stress. Exposure to high shear stress resulted in an increased association of myosin light chain kinase and protein phosphatase types 1 and 2A with the cytoskeleton, while the cytoskeletal association of protein kinase C was not evident. These results indicate that 20 kDa MLC phosphorylation is involved in shear stress-induced platelet activation, and that cytoskeletal association of protein phosphatases may regulate the phosphorylation level of cytoskeletal elements such as myosin together with myosin light chain kinase.
Nakai
K
K
2nd Department of Internal Medicine, Mie University School of Medicine, Japan.
Hayashi
T
T
Nagaya
S
S
Toyoda
H
H
Yamamoto
M
M
Shiku
H
H
Ikeda
Y
Y
Nishikawa
M
M
D001792:Blood Platelets / Q000201:enzymology / Q000378:metabolism*; D003599:Cytoskeleton / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D018994:Myosin Light Chains / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D015539:Platelet Activation / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism; D013314:Stress, Mechanical
D018994:Myosin Light Chains; D011493:Protein Kinase C; D009219:Myosin-Light-Chain Kinase; D010749:Phosphoprotein Phosphatases