Receptor-'Ck' dependent signalling regulates the LDL-receptor gene transcription.
Evidence of a novel receptor, having affinity for cholesterol in lipoproteins and intrinsic tyrosine kinase activity (designated as Receptor 'Ck') prompted us to explore its role in LDL-dependent transcriptional regulation of apoprotein-B specific LDL receptor gene in human lymphocytes. The results of this study revealed that LDL dependent activation of this Receptor 'Ck' was essential for the regulation of apoprotein-B specific LDL receptor gene transcription. Further, by using various blockers of as well as simulating the Receptor 'Ck'-dependent signalling in human lymphocytes, we were able to show that Receptor 'Ck'-dependent signalling was involved in the LDL-dependent transcriptional regulation of 160 kDa apoprotein B specific LDL receptor gene. Based upon these results, we conclude that cholesterol derived from LDL. catabolism within the cell does not have any role in the transcriptional regulation of apoprotein B specific LDL receptor gene.
Goel
R
R
Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Singh
J
J
Kaul
D
D
D001055:Apolipoproteins B / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008074:Lipoproteins / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics*; D015398:Signal Transduction; D014158:Transcription, Genetic
D001055:Apolipoproteins B; D008074:Lipoproteins; D011973:Receptors, LDL; D011505:Protein-Tyrosine Kinases