Glomerular proliferative activity and T lymphocyte infiltration in acute serum sickness.
Because hypercellularity is an important feature in acute serum sickness (AcSS), we quantified glomerular proliferation with immunoperoxidase staining using anti-proliferating cell nuclear antigen (PCNA Mab) and studied its relationship with lymphocyte infiltration (M108 Mab). AcSS was induced in 31 New Zealand White rabbits; group A (n = 14), with proteinuria, sacrificed 6-8 days after immunization; group B (n = 10), without proteinuria, sacrificed 6-7 days after immunization; group C (n = 7), sacrificed prior to development of AcSS. Four normal rabbits were included as controls. Intraglomerular proliferation (PCNA-positive cells/glomerular cross section) was increased in group A (12.2 +/- SEM, 1.84) but not in groups B (0.93 +/- 0.17) and C (0.37 +/- 0.05), which were similar to controls (0.66 +/- 0.06). Lymphocyte infiltration (lymphs/glomerular cross section) increased with time and was more prominent in rabbits with proteinuria (1.9 +/- 0.21, P < 0.001 vs controls). Lymphocyte infiltration was correlated with proliferative activity (Spearman correlation, r = 0.67, P < 0.0001). There was correlation between the severity of glomerular deposition of IgG and C3 and glomerular proliferation and proteinuria. These studies demonstrate a chronological association between lymphocyte infiltration and proliferative activity in AcSS.
Borges
G P
GP
Centro de Cirugía Experimental, Hospital Universitario de Maracaibo, Universidad del Zulia, Venezuela.
Moreno
P
P
Rodríguez Iturbe
B
B
D000208:Acute Disease; D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011507:Proteinuria / Q000473:pathology / Q000503:physiopathology; D011817:Rabbits; D012713:Serum Sickness / Q000473:pathology* / Q000503:physiopathology*; D013601:T-Lymphocytes / Q000473:pathology*
D000936:Antigen-Antibody Complex; D018809:Proliferating Cell Nuclear Antigen