title: Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens.
abstract: The gene encoding the soluble pyridine nucleotide transhydrogenase (STH) of Pseudomonas fluorescens was cloned and expressed in Escherichia coli. STH is related to the flavoprotein disulfide oxidoreductases but lacks one of the conserved redox-active cysteine residues. The gene is highly similar to an E. coli gene of unknown function.
authors.affiliation: Institute of Biotechnology, University of Cambridge, United Kingdom.
authors.forename: C E
authors.initials: CE
authors.lastname: French
authors.forename: B
authors.initials: B
authors.lastname: Boonstra
authors.forename: K A
authors.initials: KA
authors.lastname: Bufton
authors.forename: N C
authors.initials: NC
authors.lastname: Bruce
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D015870:Gene Expression; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009250:NADP Transhydrogenases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D011108:Polymers; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D011108:Polymers; D011993:Recombinant Fusion Proteins; D009250:NADP Transhydrogenases