title: On-line column-switching high-performance liquid chromatography analysis of cardiovascular drugs in serum with automated sample clean-up and zone-cutting technique to perform chiral separation.
abstract: A selective and highly reproducible, multi-column HPLC method is described for the analysis of the following cardiovascular drugs: lidocaine, pindolol, metoprolol, oxprenolol, diltiazem and verapamil, in serum. Column-switching devices are employed in combination with advanced separation media technologies for the automated analysis of samples containing complex matrices. The method consists of on-line sample clean-up using a restricted access sorbent, HPLC analysis of the drugs on a microsphere non-porous silica RP-18 column, and front-cutting to perform the chiral separation of pindolol enantiomers on a second HPLC system. Simultaneous control of the two HPLC systems and data analysis is achieved from a single centralized software. The R.S.D. values of the peak areas for spiked serum are less than 1% for metoprolol and oxprenolol, 2-5% for lidocaine, diltiazem and verapamil, and 1.2 and 2.4% for the two pindolol enantiomers. Recoveries, limits of detection and linearities are provided.
authors.affiliation: Gilson Medical Electronics (France) S.A., Villiers-le-Bel, France.
authors.forename: F
authors.initials: F
authors.lastname: Mangani
authors.forename: G
authors.initials: G
authors.lastname: Luck
authors.forename: C
authors.initials: C
authors.lastname: Fraudeau
authors.forename: E
authors.initials: E
authors.lastname: Vérette
mesh_terms: D002317:Cardiovascular Agents / Q000097:blood* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004110:Diltiazem / Q000097:blood / Q000737:chemistry; D008012:Lidocaine / Q000097:blood / Q000737:chemistry; D016014:Linear Models; D008790:Metoprolol / Q000097:blood / Q000737:chemistry; D009862:Online Systems; D010096:Oxprenolol / Q000097:blood / Q000737:chemistry; D010869:Pindolol / Q000097:blood / Q000737:chemistry; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D014700:Verapamil / Q000097:blood / Q000737:chemistry
chemical_list: D002317:Cardiovascular Agents; D010096:Oxprenolol; D008012:Lidocaine; D010869:Pindolol; D014700:Verapamil; D004110:Diltiazem; D008790:Metoprolol