Determination of lysergide in urine by high-performance liquid chromatography combined with electrospray ionisation mass spectrometry.
A quantitative method which avoids derivatisation is described for the determination of lysergide (LSD) levels in urine. Sample preparation included addition of methysergide as an internal standard followed by solid-phase extraction. LSD was analysed on a system consisting of a C18 stationary phase and a mobile phase of 0.1 M acetate buffer pH 8.0-acetonitrile-triethylamine (75:25:0.25, v/v). LSD was detected by electrospray ionisation mass spectrometry with selected ion monitoring. The quantification limit was 0.5 ng/ml and the method was linear up to 10 ng/ml of LSD in urine.
White
S A
SA
Laboratory of the Government Chemist, Teddington, Middlesex, UK.
Catterick
T
T
Harrison
M E
ME
Johnston
D E
DE
Reed
G D
GD
Webb
K S
KS
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008238:Lysergic Acid Diethylamide / Q000652:urine*
D008238:Lysergic Acid Diethylamide