High-precision, cost-effective cutting system for producing thin sections of oral tissues containing dental implants.
A new high-precision, cost-effective cutting system, able to produce thin (20-30 microns) sections of oral tissues, containing dental implants, is presented. With this system the authors have been able to obtain, in a reproducible and consistent way, thin slides containing biomaterials and biological tissues. Very high cellular detail was possible, even at high magnifications (x 1200). Histochemical reactions (e.g. acid and alkaline phosphatases) have also been obtained in a reproducible way, without sputter ground staining. The time involved in specimen cutting was significantly reduced, and about five slides per specimen were obtained with minimal loss of tissue. In almost all cases it has been possible to eliminate the grinding process with its inherent problems, such as formation of ledging at the bone-titanium interface. With this system it was also possible to obtain thin slides of soft tissue lesions (i.e. benign mucous membrane pemphigoid).
Piattelli
A
A
Dental School, University of Chieti, Italy.
Scarano
A
A
Quaranta
M
M
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D016477:Artifacts; D001331:Automation; D001672:Biocompatible Materials; D001861:Bone Regeneration; D015921:Dental Implants; D004210:Dissection / Q000295:instrumentation* / Q000379:methods; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D008252:Macaca fascicularis; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D015203:Reproducibility of Results; D014025:Titanium; D014945:Wound Healing
D001672:Biocompatible Materials; D015921:Dental Implants; D014025:Titanium; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase