Ultrasound permeabilizes CHO cells for the endonucleases AluI and benzon nuclease.
Ultrasound permeabilizes Chinese hamster ovary (CHO) cells for the endonucleases AluI and benzon nuclease which leads to the induction of chromosomal aberrations by these enzymes. A few aberrant cells were observed when trypsinized cells or adherent cells were exposed to the enzymes in the absence of ultrasound. Our data show that sonication can be used to introduce endonucleases into CHO cells. We further demonstrate that few cells can internalize endonucleases without previous permeabilization.
Johannes
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University of Essen, Department of Genetics, Germany.
Obe
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D000818:Animals; D016466:CHO Cells; D002463:Cell Membrane Permeability; D002869:Chromosome Aberrations; D006224:Cricetinae; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism* / Q000633:toxicity; D004720:Endonucleases / Q000378:metabolism* / Q000633:toxicity; D012706:Serratia marcescens / Q000201:enzymology; D014357:Trypsin / Q000494:pharmacology; D014465:Ultrasonics
D004720:Endonucleases; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; D014357:Trypsin