Two-kidney, one clip and one-kidney, one clip hypertension in mice.
The mouse remains the animal of choice in transgenic experiments, creating a need for methods of evaluating the physiology of genetically modified animals. We have established and characterized two murine models of renovascular hypertension known as the two-kidney, one clip and one-kidney, one clip models. The appropriate size of the clip lumen needed to induce high blood pressure was determined to be 0.12 mm. Clips with a lumen of 0.11 mm induced a high percentage of renal infarction, and clips with a 0.13-mm opening did not produce hypertension. Four weeks after clipping, two-kidney, one clip hypertensive mice exhibited blood pressure approximately 20 mm Hg higher than their sham-operated controls. After a similar period, this increase reached almost 35 mm Hg in the one-kidney, one clip model. Depending on the model, mice develop either renin-dependent or renin-independent hypertension. Both models are characterized by the development of cardiovascular hypertrophy.
Wiesel
P
P
Division of Hypertension, University of Lausanne (Switzerland) Medical School, Switzerland.
Mazzolai
L
L
Nussberger
J
J
Pedrazzini
T
T
D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure; D015152:Blotting, Northern; D006332:Cardiomegaly / Q000209:etiology / Q000503:physiopathology; D006339:Heart Rate; D006439:Hemodynamics; D006978:Hypertension, Renovascular / Q000097:blood / Q000235:genetics / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis; D012083:Renin / Q000097:blood / Q000235:genetics
D012313:RNA; D012083:Renin