Genomic mapping of chemokine and transforming growth factor genes in swine.
Five chemokine genes, transforming growth factors alpha, beta 2 and 3 (TGFBA, TGFB-2, and TGFB-3), interleukin 8 (IL-8), and monocyte chemoattractant protein 2 (MCP-2), were mapped to porcine linkage groups on Chromosomes 3q, 10p, 7q, 8, and 12q, respectively. Restriction fragment length polymorphisms (RFLPs) for these genes were developed by Southern blot hybridization after digestion of porcine genomic DNA with BamHI and MspI (TGFBA), BamHI and PvuII (TGFB-2), HindIII (TGFB-3), BglII (IL-8), and PstI (MCP-2) and used to genotype the USDA-MARC Swine Reference Population pigs. Sufficient informative meioses, 61 (TGFBA), 58 (TGFB-2), 28 (TGFB-3), 38 (IL-8), and 156 (MCP-2), were available to pursue two-point pairwise linkage analysis with over 1,000 existing loci in the USDA-MARC genome database to establish initial linkage (LOD > 3). Multi-point analysis with CRIMAP determined the most likely order for each new marker. The assignment of the five chemokine genes in swine concurs with previous porcine/human chromosomal homologies based on results from ZOO-FISH and chromosomal painting experiments. These findings add five new informative Type I markers within a single gene family to the swine genome and may help us understand the genetic basis for disease resistance in livestock.
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U.S. Dept. of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, P.O. Box 166, Clay Center, Nebraska 68933-0166, USA.
Rohrer
G A
GA
Stone
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Murtaugh
M P
MP
Beattie
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CW
D000818:Animals; D054412:Chemokine CCL8; D018925:Chemokines / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D008040:Genetic Linkage; D016209:Interleukin-8 / Q000235:genetics; D018945:Monocyte Chemoattractant Proteins / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000235:genetics*; D016211:Transforming Growth Factor alpha / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics; D015291:Transforming Growth Factors / Q000235:genetics*
C515453:CCL8 protein, human; D054412:Chemokine CCL8; D018925:Chemokines; D016209:Interleukin-8; D018945:Monocyte Chemoattractant Proteins; D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta; D015291:Transforming Growth Factors