Genomic organization of the human excitatory amino acid transporter gene GLT-1.
We present the genomic structure of the human glutamate transporter GLT-1 coding region, the intronic sequences adjacent to the exons, and oligonucleotide primer sequences for single strand conformational analysis. The exon-intron boundaries were determined using long-distance PCR and direct sequencing. The human GLT-1 coding region is composed of 10 exons spanning > 50 kb of genomic DNA. The exons range from 127 to 251 bp in length. The intron lengths vary considerably from 2.2 kb to > 15 kb. These data provide the basis for implementing a comprehensive screen for genetic alterations in the human GLT-1 gene using genomic DNA as a template.
Meyer
T
T
Neurologische Klinik, Universitätsklinikum Charité, Humboldt-Universität Berlin, Germany.
Ludolph
A C
AC
Morkel
M
M
Hagemeier
C
C
Speer
A
A
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000235:genetics*; D027322:Amino Acid Transport System X-AG; D001483:Base Sequence; D001692:Biological Transport; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D017931:DNA Primers; D005091:Exons; D015894:Genome, Human; D006801:Humans; D007438:Introns; D016133:Polymerase Chain Reaction
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D017931:DNA Primers