Glycosaminoglycan-biosynthesis in the wall of the umbilical cord artery and its alteration in EPH-gestosis.
The mechanism of edema, proteinuria, hypertension (EPH)-gestosis-associated premature replacement of hyaluronic acid by sulphated glycosaminoglycans (GAGs) in the umbilical cord arteries is not known. It may result from altered biosynthesis, a different degradation rate or a combination of both phenomena. In order to solve this problem, it was decided to evaluate the biosynthesis and degradation of newly synthesized GAGs in the umbilical cord arteries of control newborns and those delivered by mothers with EPH-gestosis. Incorporation of radioactive precursors ([14C]glucosamine and [35S]sulphate) into GAGs and degradation of newly synthesized GAGs using the pulse-chase experiment were evaluated. We found that the investigated tissue slices incorporated distinctly less [14C]glucosamine into hyaluronic acid in comparison to controls. In contrast to that, the biosynthesis of sulphated GAGs did not change significantly. However, the degradation of newly synthesized sulphated GAGs was distinctly slower than in control tissues. It may be concluded that an EPH-gestosis associated decrease in hyaluronic acid content in the umbilical cord artery is a result of decreased biosynthesis of this substance, whereas an increase in sulphated GAGs-content is rather a result of slower degradation of newly synthesized GAGs.
Romanowicz
L
L
Department of Biochemistry, Medical Academy of Białystok, Poland.
Bańkowski
E
E
Galewska
Z
Z
Jaworski
S
S
D000293:Adolescent; D000328:Adult; D002250:Carbon Radioisotopes; D005260:Female; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D007231:Infant, Newborn; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014469:Umbilical Arteries / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D005944:Glucosamine