title: The prevention of graft-versus-host disease by the serum of liver retransplanted rats.
abstract: The effect of serum from orthotopic liver retransplanted rats (re-OLT serum) on graft-versus-host disease (GVHD) was studied in rats. In the re-transplantation model of rat liver, orthotopic liver transplantation (OLT) was carried out in the DA (RT1a) into PVG (RT1c) combination; two days later the DA liver was removed and a new PVG liver implanted into the same recipient (re-OLT). In the in vivo GVHD model, male PVG rats were sublethally irradiated and injected intravenously with 3 x 10(8) DA or BN (RT1n) spleen through the penial vein. Within 1 h of the inoculation, rats of the experimental group were injected with 1 ml of re-OLT serum taken at postoperative day (POD) 7. Rats in the control group received 1 ml of normal PVG serum or syngeneic re-OLT serum (PVG-PVG, PVG-PVG). All PVG rats in the control groups died of GVHD within 21 days after the inoculation of DA or BN spleen lymphocytes. However, when the animals were treated with re-OLT serum, 100% (6/6) of the rats survived more than 60 days, following inoculation with DA lymphocytes but not with BN lymphocytes. The POD 7 re-OLT serum showed a strong inhibition against DA anti-PVG mixed lymphocyte reaction (MLR), although re-OLT serum did not contain soluble DA class I antigens, anti-DA class I or II antibody. The potential GVHD inhibitory factors in re-OLT serum may be two unique immunosuppressive proteins, which have been detected by SDS PAGE and reported previously. We conclude that re-OLT serum has immunosuppressive factors, which, at least in part, prevented the induction of GVHD in rats.
authors.affiliation: Department of Surgery, University of Queensland, Brisbane, Australia.
authors.forename: Y
authors.initials: Y
authors.lastname: Shimizu
authors.forename: S
authors.initials: S
authors.lastname: Goto
authors.forename: F
authors.initials: F
authors.lastname: Vari
authors.forename: R
authors.initials: R
authors.lastname: Lord
authors.forename: C
authors.initials: C
authors.lastname: Edwards-Smith
authors.forename: S
authors.initials: S
authors.lastname: Chiba
authors.forename: D
authors.initials: D
authors.lastname: Schlect
authors.forename: M
authors.initials: M
authors.lastname: Buckley
authors.forename: M
authors.initials: M
authors.lastname: Kusano
authors.forename: N
authors.initials: N
authors.lastname: Kamada
mesh_terms: D000818:Animals; D006086:Graft vs Host Disease / Q000097:blood / Q000517:prevention & control*; D007166:Immunosuppressive Agents / Q000097:blood*; D016031:Liver Transplantation / Q000276:immunology*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D012086:Reoperation; D014184:Transplantation, Homologous
chemical_list: D007166:Immunosuppressive Agents