Analysis of dipalmitoyl phosphatidylcholine in amniotic fluid by high-performance liquid chromatography.
A novel method for the determination of dipalmitoyl phosphatidylcholine (DPPC) in amniotic fluid by high-performance liquid chromatography (HPLC) is described. Aliquots of 50 microliters of amniotic fluid were hydrolyzed with phospholipase C from Bacillus cereus and the resulting dipalmitoylglycerol analyzed by HPLC. Run-to-run and day-to-day precision for DPPC analysis were 4.2 and 6.1%, respectively, and analysis time was 10 min. Recoveries for DPPC ranged between 92 and 98%. In summarizing, this method provides a high precision and fast turnaround time means for the analysis of DPPC in amniotic fluid.
Alvarez
J G
JG
Department of Obstetrics and Gynecology, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215, USA.
Slomovic
B
B
Ludmir
J
J
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000032:analysis*; D000653:Amniotic Fluid / Q000737:chemistry* / Q000378:metabolism; D001409:Bacillus cereus / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D010738:Type C Phospholipases / Q000378:metabolism
D015060:1,2-Dipalmitoylphosphatidylcholine; D010738:Type C Phospholipases