Role of microtubules and microtubule organizing centers on meiotic chromosome elimination in Sciara ocellaris.
Spindle formation and chromosome elimination during male meiosis in Sciara ocellaris (Diptera, Sciaridae) has been studied by immunofluorescence techniques. During meiosis I a monopolar spindle is formed from a single polar complex (centrosome-like structure). This single centrosomal structure persists during meiosis II and is responsible for the non-disjunction of the maternal X chromatids. During meiosis I and II non-spindle microtubules are assembled in the cytoplasmic bud regions of the spermatocytes. The chromosomes undergoing elimination during both meiotic divisions are segregated to the bud region where they associate with bundles of microtubules. The presence and distribution of centrosomal antigens in S. ocellaris meiotic spindles and bud regions has been investigated using different antibodies. gamma-Tubulin and centrin are present in the bud as well as in the single polar complex of first meiotic spindle. The results suggest that spermatocyte bud regions contain microtubule-organizing centres (MTOCs) that nucleate cytoplasmic microtubules that are involved in capturing chromosomes in the bud regions. The distribution of actin and myosin in the spermatocytes during meiosis is also reported.
Esteban
M R
MR
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Campos
M C
MC
Perondini
A L
AL
Goday
C
C
D000199:Actins / Q000378:metabolism; D000818:Animals; D018385:Centrosome / Q000378:metabolism; D002872:Chromosome Deletion; D004175:Diptera / Q000166:cytology / Q000235:genetics*; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D008540:Meiosis / Q000235:genetics*; D008870:Microtubules / Q000502:physiology*; D009218:Myosins / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000276:immunology; D014404:Tubulin / Q000276:immunology
D000199:Actins; D014404:Tubulin; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D009218:Myosins