Immune effector cells induced by complete Freund's adjuvant exert an inhibitory effect on antigen-specific type 2 T helper responses.
BACKGROUND
It has been well documented that environmental factors such as antigenpresenting cells and related cytokines could affect the development of T helper cells.


OBJECTIVE
The purpose of this study is to investigate the effect of different adjuvants on T cell development.


METHODS
Ovalbumin (OVA) combined with aluminum hydroxide (Alum) plus pertussis toxin (PT) or complete Freund's adjuvant (CFA) were used to sensitize mice; the production of IgG and IgE anti-OVA antibodies was then followed. In addition, OVA-specific proliferative responses and cytokine production by spleen cells were also investigated.


RESULTS
The data showed that the adjuvants themselves could modify the pattern of immune response: (1) IgG2a anti-OVA antibody was higher in mice sensitized with OVA + CFA compared to that of mice sensitized with OVA + Alum + PT; (2) the ratio of IFN-gamma/IL-4 produced by OVA-stimulated spleen cells was higher in mice sensitized with OVA + CFA than that of mice sensitized with OVA + Alum + PT: (3) increased percentage of gamma delta T cells was noted in the peritoneal exudate cells of OVA + CFA immunized mice; and (4) the immune response of mice sensitized with OVA + Alum + PT was inhibited by the adoptively transferred ascitic cells from OVA + CFA immunized mice.


CONCLUSIONS
In general, the data suggested higher IgG2a and the ratio of IFN-gamma/IL-4 was noted in mice sensitized with OVA + CFA. Further elucidation of the regulatory mechanism of allergen-specific T helper cells development and exploration of possible agents for immunotherapy might shed light on the management of atopic diseases.
Chuang
Y H
YH
Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Chiang
B L
BL
Chou
C C
CC
Hsieh
K H
KH
D000536:Aluminum Hydroxide / Q000008:administration & dosage / Q000276:immunology; D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D005620:Freund's Adjuvant / Q000008:administration & dosage / Q000276:immunology*; D016130:Immunophenotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D037342:Pertussis Toxin; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology*; D010566:Virulence Factors, Bordetella / Q000008:administration & dosage / Q000276:immunology
D016692:Receptors, Antigen, T-Cell, gamma-delta; D010566:Virulence Factors, Bordetella; D000536:Aluminum Hydroxide; D010047:Ovalbumin; D005620:Freund's Adjuvant; D037342:Pertussis Toxin