Altered Th1/Th2 balance associated with non-major histocompatibility complex genes in collagen-induced arthritis in resistant and non-resistant rat strains.
Collagen-induced arthritis (CIA) is a T cell-dependent disease in which susceptibility is controlled by genes both within and outside the major histocompatibility complex (MHC). In the present study, we compared the humoral responses and kinetics of cytokine secretion patterns in the draining lymph nodes of arthritis-susceptible DA rats and arthritis-resistant F344 and DA MHC congenic PVG.1AV1 rats immunized with rat type II collagen (RCII) in incomplete Freund's adjuvant. The results demonstrate a marked humoral RCII response and a Th1 cytokine profile, with expression of interferon-gamma and interleukin (IL)-2 mRNA in DA rats; a limited humoral RCII response and a Th2 cytokine profile, with expression of IL-4 mRNA in arthritis-resistant F344 rats; and a marked humoral RCII response in arthritis-resistant PVG.1AV1 rats. However, in contrast to DA rats, PVG.1AV1 rats produce IgG1 autoantibodies which, together with strong expression of IL-4 mRNA, indicates the involvement of Th2 subsets. From these data, we conclude that non-MHC gene(s) determines the direction of the anti-RCII response towards a Th1 disease-promoting, or a Th2 disease-limiting response.
Müssener
A
A
Department of Rheumatology, Karolinska Hospital, Stockholm, Sweden. muessner@minerva.data.ks.se
Lorentzen
J C
JC
Kleinau
S
S
Klareskog
L
L
D000818:Animals; D000917:Antibody Formation; D001169:Arthritis, Experimental / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis; D003094:Collagen / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D008198:Lymph Nodes / Q000276:immunology; D008285:Major Histocompatibility Complex; D010047:Ovalbumin / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344 / Q000276:immunology; D011919:Rats, Inbred Strains; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D001323:Autoantibodies; D007132:Immunoglobulin Isotypes; D007376:Interleukin-2; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007371:Interferon-gamma; D010047:Ovalbumin; D003094:Collagen