title: Identification of variant translocations in chronic myeloid leukemia by fluorescence in situ hybridization.
abstract: We studied two cases of chronic myeloid leukemia (CML) having variant complex translocations detected by trypsin G-banding and fluorescence in situ hybridization (FISH). Application of dual color- (DC-) FISH using abl and bor cosmid probes permitted us to detect the bor-abl fusion event on both interphase nuclei and metaphase spread. Furthermore, FISH using combinatorial hybridization (centromeric-library and library-library probes) demonstrated the content and the position of the translocations in CML patients with variant (complex type) Ph-positive rearrangements. FISH analysis appears to be superior than conventional cytogenetic analysis.
authors.affiliation: Department of Medical Genetics, University of Selçuk, Konya, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Acar
authors.forename: J
authors.initials: J
authors.lastname: Stewart
authors.forename: E
authors.initials: E
authors.lastname: Boyd
authors.forename: M J
authors.initials: MJ
authors.lastname: Connor
mesh_terms: D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D010677:Philadelphia Chromosome; D014178:Translocation, Genetic / Q000235:genetics*