Multiple loci of Pseudomonas syringae pv. syringae are involved in pathogenicity on bean: restoration of one lesion-deficient mutant requires two tRNA genes.
A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant strains were defective in the ability to elicit the hypersensitive reaction (HR) and were shown to contain Tn5 insertions within the P. syringae pv. syringae hrp region. Ten HR+ mutants were defective in the production of the toxin syringomycin, and a region of the chromosome implicated in the biosynthesis of syringomycin was deleted in a subset of these mutants. The remaining seven lesion-defective mutants retained the ability to produce protease and syringomycin. Marker exchange mutagenesis confirmed that the Tn5 insertion was causal to the mutant phenotype in several lesion-defective, HR+ strains. KW239, a lesion- and syringomycin-deficient mutant, was characterized at the molecular level. Sequence analysis of the chromosomal region flanking the Tn5 within KW239 revealed strong similarities to a number of known Escherichia coli gene products and DNA sequences: the nusA operon, including the complete initiator tRNA(Met) gene, metY; a tRNA(Leu) gene; the tpiA gene product; and the MrsA protein. Removal of sequences containing the two potential tRNA genes prevented restoration of mutant KW239 in trans. The Tn5 insertions within the lesion-deficient strains examined, including KW239, were not closely linked to each other or to the lemA or gacA genes previously identified as involved in lesion formation by P. syringae pv. syringae.
Rich
J J
JJ
Department of Plant Pathology, University of Wisconsin-Madison, 53706, USA.
Willis
D K
DK
D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D011549:Pseudomonas / Q000235:genetics / Q000472:pathogenicity*; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012358:RNA, Transfer, Met / Q000235:genetics*; D015183:Restriction Mapping; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; C091100:GacA protein, Bacteria; D012356:RNA, Transfer, Leu; D012358:RNA, Transfer, Met; D014157:Transcription Factors; C074165:lemA protein, bacterial; D010450:Endopeptidases