BMPs induce endochondral ossification in rats when implanted ectopically within a carrier made of fibrous glass membrane.
Bone morphogenetic proteins (BMPs) replicate the process of embryonic bone formation when implanted in ectopic sites. Our previous studies have indicated that BMPs can induce intramembranous ossification, i.e., direct bone formation without preexisting cartilage when implanted in rats subcutaneously by using the fibrous collagen membrane (FCM) as a carrier for implanting BMPs (Sasano et al. 1993. Anat. Rec., 236:373-380). The present study was designed to investigate how the physicochemical property of the carrier material influences the process of bone formation induced by BMPs, using a carrier made of fibrous glass membrane (FGM).



BMPs, partially purified from bovine metatarsal bones, were added to an FGM carrier and implanted subcutaneously in rats. The implants were analyzed at weekly intervals, and the osteogenic process induced by BMPs was examined by histology and immunohistochemistry for cartilage and bone formation.



Neither cartilage nor bone were observed after week 1. Cartilage formation occurred within the carrier after week 2, although no bone formation was seen. The cartilage matrix showed immunoreactivity for types II, X, and I collagen. Bone was induced on the previously formed cartilage after week 3. The bone matrix stained with anti-osteocalcin antibody and with anti-type I collagen antibody. The cartilage was replaced by bone and bone marrow after week 10.



BMPs cause endochondral ossification when administered with an FGM carrier. The physicochemical property of the carrier may be involved in the BMP-induced phenotype expression of bone and cartilage.
Sasano
Y
Y
Second Department of Oral Anatomy, Tohoku University School of Dentistry, Sendai, Japan.
Mizoguchi
I
I
Takahashi
I
I
Kagayama
M
M
Saito
T
T
Kuboki
Y
Y
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002356:Cartilage / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D005898:Glass; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008297:Male; D009999:Ossification, Heterotopic / Q000139:chemically induced* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; C009753:fiberglass; D015675:Osteocalcin; D003094:Collagen