Comparison and evaluation of the specificity and binding capacity of commercial and in house affinity columns used in sample preparation for analysis of growth-promoting drugs.
Immunoaffinity chromatography (IAC) and affinity chromatography (AC) are widely used for extraction of drugs from biological samples. Fifteen column types were purchased from five different manufacturers and their ability to bind specific drugs including beta-agonists and anabolic steroids over a range of analyte concentrations in fortified bovine urine samples was assessed. The performance data obtained from these columns were compared with columns produced in this laboratory (in house columns). The in house columns gave the highest recoveries, ranging from 92 to 100% at the 1 ng spiking concentration, for five of the seven analytes assessed. Forty percent (11 of 27) of all the commercial column assessments recorded recoveries of less than 50% even when the lowest spiking concentration was applied (1 ng). For one manufacturer, only one of seven different columns purchased delivered extraction efficiencies greater than 50%. The extraction efficiencies of the clenbuterol columns were the highest with all commercially prepared columns showing at least 50% binding of radiolabelled tracer. Recoveries of alpha-nortestosterone were the lowest. The variability of these products with respect to quality control requires constant monitoring.
Crooks
S R
SR
Residue Laboratory, Veterinary Sciences Division, Belfast, N. Ireland, UK.
Elliott
C T
CT
Thompson
C S
CS
McCaughey
W J
WJ
D000318:Adrenergic beta-Agonists / Q000276:immunology / Q000652:urine*; D045930:Anabolic Agents / Q000276:immunology / Q000652:urine*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D002846:Chromatography, Affinity / Q000295:instrumentation*; D004968:Estrogens, Non-Steroidal / Q000276:immunology / Q000652:urine*; D012680:Sensitivity and Specificity
D000318:Adrenergic beta-Agonists; D045930:Anabolic Agents; D000906:Antibodies; D004968:Estrogens, Non-Steroidal