DNA extraction by zinc.
A fast, very simple and efficient method of DNA extraction is described which takes advantage of DNA sedimentation induced by millimolar concentrations of ZnCl2. The zinc-induced sedimentation is furthermore strongly promoted by submillimolar phosphate anion concentrations. Within <30 min, the method recovers >90% of DNA irrespective of whether a plasmid DNA or short oligonucleotides are the extracted material. The method works with plasmid DNA and oligonucleotide concentrations as low as 100 ng/ml and 10 microg/ml, respectively, without using any expensive facilities or toxic chemicals.
Kejnovský
E
E
Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265 Brno, Czech Republic.
Kypr
J
J
D002021:Buffers; D002712:Chlorides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D007202:Indicators and Reagents; D017967:Zinc Compounds / Q000737:chemistry*
D002021:Buffers; D002712:Chlorides; D007202:Indicators and Reagents; D017967:Zinc Compounds; C016837:zinc chloride; D004247:DNA