[Molecular analysis of enterohemorrhagic Escherichia coli O157:H7 isolates in Japan 1996 using pulsed-field gel electrophoresis].
Using pulsed-field gel electrophoresis(PFGE) with XbaI digestion, we analyzed 1,794 enterohemorrhagic Escherichia coli(EHEC) O157 isolates, which were derived from 16 outbreaks, sporadic cases, foods, beef fecal swabs, and environments in 1996 in Japan. They were classified into six types according to observed PFGE patterns. EHEC O157:H7 isolates from seven out of the 16 outbreaks showed very closely related patterns and those from other five outbreaks did the same pattern; in the former outbreaks, no common source could be identified, while in the latter outbreaks, radish sprout is thought to be the common cause of the infection. PFGE patterns of the remaining four outbreaks were not correlated to each other.
Wada
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Department of Bacteriology, National Institute of Health Japan.
D000818:Animals; D015415:Biomarkers; D002417:Cattle; D003092:Colitis / Q000453:epidemiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005516:Food Microbiology; D005798:Genes, Bacterial; D006801:Humans; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction
D015415:Biomarkers; D004269:DNA, Bacterial