Presentation of ligands on hydroxylapatite.
Conjugates of biotin with the decamer of glutamic acid (glu10) and the trimer of D,L-2-amino-5-phosphonovaleric acid (I) have been synthesized, and it has been shown that they mediate the binding of avidin to hydroxylapatite. In a similar way a conjugate of methotrexate with glu10 mediates the binding of dihydrofolate reducatase to the mineral. The presentation of ligands on the hydroxylapatite component of bone may find applications in clinical medicine.
Chu
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Salk Institute for Biological Studies, San Diego, California 92186-5800, USA.
Orgel
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D015763:2-Amino-5-phosphonovalerate / Q000378:metabolism; D000327:Adsorption; D000818:Animals; D001360:Avidin / Q000378:metabolism; D001665:Binding Sites; D001842:Bone and Bones / Q000378:metabolism; D002846:Chromatography, Affinity; D017886:Durapatite / Q000378:metabolism; D066298:In Vitro Techniques; D008024:Ligands; D008727:Methotrexate / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism
D008024:Ligands; D009842:Oligopeptides; D001360:Avidin; D015763:2-Amino-5-phosphonovalerate; D017886:Durapatite; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate