Sulfatides inhibit binding of Helicobacter pylori to the gastric cancer Kato III cell line.
Helicobacter pylori adhere to Kato III and Hela S3 cells in monolayer cultures. To explore whether cell surface glycoconjugates on these two cell lines mediate binding of H. pylori, various carbohydrates, glycoproteins, and glycolipids were tested to inhibit H.pylori cell adhesion. The adhesion was measured (i) with a urease-based assay and (ii) by cells stained with fluorescein. Sodium periodate and sialidase treatment (but not alpha- or beta-galactosidase, heparitinase,lysozyme, or trypsin) inhibited H. pylori binding to both cell lines. Sulfatides and sulfated glycoconjugates (50 microg/ml) but not heparin or a number of simple carbohydrates inhibited binding (1 mg/ml). The two H.pylori strains studied (CCUG 17874 and strain 25) showed high binding of soluble 125I-labeled heparin and other sulfated carbohydrate compounds.
Wadström
T
T
Department of Medical Microbiology, University of Lund, Sölvegatan 23, Lund S-22362, Sweden.
Hirmo
S
S
Novak
H
H
Guzman
A
A
Ringnér-Pantzar
M
M
Utt
M
M
Aleljung
P
P
D001422:Bacterial Adhesion / Q000187:drug effects*; D050260:Carbohydrate Metabolism; D006001:Glycoconjugates / Q000494:pharmacology; D006367:HeLa Cells; D016480:Helicobacter pylori / Q000187:drug effects* / Q000378:metabolism; D006493:Heparin / Q000378:metabolism; D006801:Humans; D009439:Neuraminidase / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000382:microbiology; D013433:Sulfoglycosphingolipids / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D006001:Glycoconjugates; D013433:Sulfoglycosphingolipids; D010504:Periodic Acid; D006493:Heparin; C009288:metaperiodate; D009439:Neuraminidase