title: NMR evidence for a novel asparagine-linked oligosaccharide on cellobiohydrolase I from Trichoderma reesei RUTC 30.
abstract: The primary structure of a novel phosphate-containing oligosaccharide, isolated from T. reesei cellobiohydrolase I, was determined by NMR techniques. The new compound has the same structure as GlcMan7GlcNAc2, but it is extended by one alpha-mannopyranosyl unit (Man-P) through a phosphate link. Three different heteronuclear (31P-1H) NMR techniques were used to prove that the phosphate links the glycosidic site of Man-P with C-6 of unit Man-B. The presence of mannoses linked through a phosphate diester resembles glycosyl synthesis in yeast.
authors.affiliation: Laboratory of Organic Chemistry, University of Ghent, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: De Bruyn
authors.forename: M
authors.initials: M
authors.lastname: Maras
authors.forename: J
authors.initials: J
authors.lastname: Schraml
authors.forename: P
authors.initials: P
authors.lastname: Herdewijn
authors.forename: R
authors.initials: R
authors.lastname: Contreras
mesh_terms: D001216:Asparagine / Q000032:analysis* / Q000737:chemistry; D002240:Carbohydrate Sequence; D002480:Cellulase / Q000737:chemistry*; D043366:Cellulose 1,4-beta-Cellobiosidase; D005656:Fungal Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D009844:Oligosaccharides; D001216:Asparagine; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase