Syringaldazine. New attractive electron donor of prostaglandin H synthase.
The prostaglandin H synthase (PGHS) reaction has been studied with syringaldazine as the electron donor. Kinetic parameters of the reaction agreed with those for commonly used electron donors. The sensitivity of the PGHS activity detection in the presence of syringaldazine increases 10 times. A sensitive spectrophotometric assay for polyunsaturated fatty acid (PUFA) detection using syringaldazine as a new electron donor has been proposed.
Kuznetsova
Y A
YA
A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia. julia@pghs.genebee.msu.su
Romakh
V B
VB
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000175:diagnosis*; D004796:Clinical Enzyme Tests / Q000379:methods*; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D006835:Hydrazones / Q000378:metabolism*; D007202:Indicators and Reagents / Q000378:metabolism*; D008861:Microsomes; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D012756:Sheep; D013379:Substrate Specificity
D006835:Hydrazones; D007202:Indicators and Reagents; C009370:syringaldazine; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D011451:Prostaglandin-Endoperoxide Synthases