Effects of nedocromil sodium on the oxidative burst of polymorphonuclear leukocytes: comparison with salbutamol.
The effects of nedocromil sodium and of salbutamol on the generation of oxygen-derived free radicals in human polymorphonuclear leukocytes (PMNs) were compared in vitro by the luminol-amplified-chemiluminescence (LACL) assay induced by both particulate (Candida albicans) and soluble formyl-methionyl-leucyl-phenylalanine (fMLP) stimulants. Inhibitory dose-effect linear regressions were observed from 10(-3) to 10(-8) M for nedocromil and salbutamol after a 3' period of incubation with either C. albicans or fMLP. There was a linear regression with nedocromil sodium after 30' incubation, but desensitization was observed with salbutamol after this longer period of incubation. The generation of oxygen-derived free radicals was significantly greater for asthmatic patients than for normal subjects; therefore antiasthmatic drugs with this inhibitory activity could be an extra pharmacological benefit in the treatment of asthmatic patients.
Braga
P C
PC
Department of Pharmacology, University of Milan, Italy.
Mancini
L
L
Guffanti
E E
EE
Dal Sasso
M
M
Sala
M
M
Reggio
S
S
D000420:Albuterol / Q000494:pharmacology*; D000704:Analysis of Variance; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D001993:Bronchodilator Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D016014:Linear Models; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism; D017835:Nedocromil / Q000494:pharmacology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species; D016897:Respiratory Burst / Q000187:drug effects*
D018927:Anti-Asthmatic Agents; D001993:Bronchodilator Agents; D017382:Reactive Oxygen Species; D017835:Nedocromil; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000420:Albuterol