Immunochemical characterisation of monoclonal antibodies directed to glycophorins A and/or B.
Among sixty-nine monoclonal antibodies submitted to the workshop, 28 antibodies directed to glycophorins A and/or B but without blood group specificity were investigated by a series of methods involving agglutination, flow cytometry with CHO transfected cells expressing glycophorin A, ELISA with a carbohydrate-free peptide (residues 1-72) of glycophorin A, and immunoblotting. These MAbs were subdivided in several groups according to their specificity: N-terminal portion of GPA and GPB; N-terminal trypsin-sensitive portion of GPA; extracellular ficin-sensitive portion of GPA; intracellular domain of GPA; undetermined. Both flow cytometry with transfectant cells and ELISA with the synthetic peptide prove to be of value in order to determine subspecificities within these groups.
Rasamoelisolo
M
M
Etablissement de Transfusion Sanguine de Loire Atlantique/Vendée, Site de Nantes, France.
Rahuel
C
C
Loirat
M J
MJ
Willem
C
C
Cartron
J P
JP
Blanchard
D
D
D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal; D016466:CHO Cells; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology*; D005434:Flow Cytometry; D006021:Glycophorins / Q000276:immunology*; D015151:Immunoblotting; D014162:Transfection
D000911:Antibodies, Monoclonal; D006021:Glycophorins