The mouse chromosome 7 distal imprinting domain maps to G-bands F4/F5.
Mouse Chromosome (Chr) 7 distal to band F3 on the physical map is known to be subject to imprinting, maternal duplication (MatDp) of the region leading to a late embryonic lethality, while paternal duplication (PatDp) causes death in utero before 11.5 dpc. Using a new mouse reciprocal translocation T(7;11)65H to produce MatDp for distal Chr 7, we have mapped the region subject to imprinting more precisely to bands 7F4/F5 on the cytogenetic map. Fluorescence in situ hybridization (FISH) studies on mitotic and meiotic chromosomes of a T65H heterozygote show that the imprinted gene Igf2 is located in the same region. This was confirmed by the finding that embryos with MatDp of bands 7F4/F5 did not express Igf2. We suggest that other members of the imprinted domain containing Igf2, namely Mash2, H19, Ins2, and p57(K1P2), are also located in 7F4/F5 and that some or all of these genes may be responsible for the two imprinting lethalities seen with MatDp and PatDp for this region.
Beechey
C V
CV
Mammalian Genetics Unit, Medical Research Council, Harwell, Didcot, Oxfordshire OX11 0RD, UK.
Ball
S T
ST
Townsend
K M
KM
Jones
J
J
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D004622:Embryo, Mammalian; D005260:Female; D018392:Genomic Imprinting; D017404:In Situ Hybridization, Fluorescence; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D008297:Male; D008540:Meiosis; D051379:Mice; D008938:Mitosis; D010641:Phenotype; D014178:Translocation, Genetic
D007335:Insulin-Like Growth Factor II