A new semi-automated chromosome analysis system for in vitro chromosomal aberration tests.
We have evaluated a new semi-automated chromosome analysis system, employing the Magiscan human metaphase finder, for in vitro chromosomal aberration tests. The metaphases on a slide are recognized using the main system, a metaphase finder, and their stage coordinates are transferred to the satellite system, a computerized microscope with a motorized stage, by way of a diskette. In the satellite system, a researcher analyzes one metaphase after another at high power (100 x objective) without changing the objective. The effectiveness of the system, in comparison with the manual metaphase finding and analysis, was confirmed in in vitro chromosomal aberration tests using cultured Chinese hamster cells. Structural or numerical aberrations in the cells did not affect the metaphase findings. The system reduces the time for chromosome analysis by a factor of about 4. Moreover, the system provides perfect reproducibility for analyzing procedure. It is concluded that this semi-automated system is useful in in vitro chromosomal aberration tests.
Odawara
K
K
Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Osaka, Japan. odawara@sc.sumitomo-chem.co.jp
Yamamoto
K
K
Kato
H
H
Hara
M
M
Kogiso
S
S
Kishida
F
F
Yoshitake
A
A
Nakatsuka
I
I
D000818:Animals; D001331:Automation; D016466:CHO Cells; D002869:Chromosome Aberrations; D006224:Cricetinae; D003520:Cyclophosphamide / Q000633:toxicity; D006801:Humans; D008168:Lung; D008677:Metaphase; D016685:Mitomycin / Q000633:toxicity; D016296:Mutagenesis; D009152:Mutagenicity Tests / Q000295:instrumentation* / Q000379:methods; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D016685:Mitomycin; D003520:Cyclophosphamide