title: Activity satellite association and polymorphism of Ag stained nucleolus organizer regions (Ag+ NORs) in lymphocytes from women with cervical uterine cancer.
abstract: Thirty five female patients with different stages of neoplastic lesions: cervical intraepithelial neoplasia (CIN) or dysplasia (CIN I and CIN II), in situ carcinoma (CIS), and adenocarcinoma, and 27 healthy women (controls) were studied to determine the activity, satellite association, and polymorphism of Ag stained nucleolus organizer regions (Ag+ NORs) in acrocentric chromosomes in metaphases obtained from peripheral blood lymphocytes. For each person, 25 to 50 metaphases stained with ammoniacal silver technique were scored. The average number of Ag+ NORs was higher in women with adenocarcinoma (7.66 +/- 0.72) than in controls (6.65 +/- 0.74). Non-associated chromosomes showing Ag+ NORs were found more frequently in patients (5.85 +/- 0.88) than in controls (4.81 +/- 0.67). Patients aged 30-39 and 60 or more had an increase of Ag+ NORs (7.99 +/- 1.04, and 7.81 +/- 0.71) with respect to their controls (6.36 +/- 0.052 and 6.17 +/- 0.88), but the frequency of satellite association showed lower values in 50-59 year-old patients (0.75 +/- 0.08) than in controls (1.02 +/- 0.19). The most frequent association in patients was the large type (patients = 38.96%, controls = 30.49%). The partial association showed higher values (6.49%) than controls (2.44%). Otherwise, the spherical association was more frequent for controls (37.80%) than for patients (28.57%). All these differences were statistically significant (p < 0.05). The frequency of Ag+ NORs and the type of polymorphism of satellite association could be related to the neoplastic process, while the frequency of satellite association and of polymorphism of Ag+ NORs seems to be irrelevant.
authors.affiliation: Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, N.L., México.
authors.forename: E I
authors.initials: EI
authors.lastname: Cortés-Gutiérrez
authors.forename: R
authors.initials: R
authors.lastname: Reyna-Hinojosa
authors.forename: J
authors.initials: J
authors.lastname: Silva-Cudish
authors.forename: M A
authors.initials: MA
authors.lastname: Rojas-Alvarado
authors.forename: C H
authors.initials: CH
authors.lastname: Leal-Garza
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000648:ultrastructure*; D000328:Adult; D002278:Carcinoma in Situ / Q000737:chemistry / Q000648:ultrastructure*; D002901:Chromosomes, Human, 13-15 / Q000648:ultrastructure*; D002904:Chromosomes, Human, 21-22 and Y / Q000648:ultrastructure*; D018450:Disease Progression; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000648:ultrastructure*; D008875:Middle Aged; D009697:Nucleolus Organizer Region / Q000737:chemistry / Q000648:ultrastructure*; D016622:Silver Staining; D002578:Uterine Cervical Dysplasia / Q000737:chemistry / Q000648:ultrastructure; D002583:Uterine Cervical Neoplasms / Q000737:chemistry / Q000648:ultrastructure*