A functional model for the ribosome.
The "ice" structure for the E. coli ribosome clearly illustrates how the ribosome could work and it is used as the basis of a detailed account of ribosome function. There have been objections to this structure as it does not fit with current beliefs about the position of the T-site, for the arriving tRNA, or the Exit-site: these beliefs are wrong. The elongation factor EF-Tu is required at both the T-site and the E-site, and these two attachment areas were combined into the false site. The E-site has been placed at the L1-ridge because deacylated tRNAs attach to protein L1, but none of these experiments used a cognate codon to get a positive identification of this as the E-site. The reason L1 attaches to deacylated tRNAs is that it is at the E-site in polysomes, which contain closely stacked, or overlapping, ribosomes with the L1-ridge at the interface between ribosomes. The revised data allows the 1989 ribosome model to be reconciled with the most contradictory results and it is then used to develop a more detailed picture of the ribosome.
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D000818:Animals; D005075:Biological Evolution; D004926:Escherichia coli / Q000648:ultrastructure*; D008954:Models, Biological; D012335:RNA, Ribosomal / Q000502:physiology; D012343:RNA, Transfer / Q000502:physiology; D012269:Ribosomal Proteins / Q000502:physiology*; D012270:Ribosomes / Q000502:physiology* / Q000648:ultrastructure
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D012343:RNA, Transfer