Purification and partial characterization of chromogranin-A from human brain.
Chromogranin A was purified from post-mortem human brain tissue, obtained at autopsy by a three step chromatography procedure to a single band purity on SDS gel electrophoresis at 68 kDa. Purified protein was further characterized by immunoblotting using a mouse anti-chromogranin A monoclonal antibody LK2H10, which recognizes the 68 kDa chromogranin A in human brain. The results demonstrate a simple protocol of preparing CgA from the human brain. Chromogranin plays a very important role in various neurological disorder and has been examined immunohistochemically in Alzheimer's disease, Parkinson's disease, Pick's disease. The procedure of purification may be useful in further defining its biological function, biochemistry and physiological significance in the central nervous system and may be useful in delineating the molecular pathology of certain neurological disorders.
Kumar
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Department Of Medicine, Royal Victoria Hospital University of McGill, Montreal, PQ, Canada.
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001923:Brain Chemistry; D002848:Chromatography, DEAE-Cellulose; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D051379:Mice
C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; C506155:chromogranin A, mouse