Molecular cloning of the human methylthioadenosine phosphorylase processed pseudogene and localization to 3q28.
Human methylthioadenosine phosphorylase (MTAP) is a purine and methionine metabolic enzyme present ubiquitously in all normal tissues, but often deleted in many types of cancer. The gene for this enzyme maps to chromosome 9 at band p21 where the cyclin-dependent kinase inhibitor genes for p16 and p15 also reside. During our efforts to clone this gene we also isolated a phage clone containing a processed pseudogene of MTAP. The sequence is 92% homologous to the MTAP cDNA, is flanked at its 3' end by a repetitive element, but does not possess a poly(A) stretch. We localized this processed pseudogene to band 28 on the long arm of chromosome 3 by fluorescence in situ hybridization. All 22 malignant cell lines with deletions at 9p21 screened possessed the pseudogene.
Tran
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PT
Department of Medicine, University of California, San Diego, La Jolla 92093-0663, USA.
Hori
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H
Hori
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Okumura
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Kagotani
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Taguchi
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Carson
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DA
Nobori
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D016368:Frameshift Mutation; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D011544:Pseudogenes; D011683:Purine-Nucleoside Phosphorylase / Q000096:biosynthesis / Q000235:genetics*; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured
D011683:Purine-Nucleoside Phosphorylase; C021784:5'-methylthioadenosine phosphorylase