title: Selective cell ablation in transgenic mice expression E. coli nitroreductase.
abstract: The gene encoding E. coli nitroreductase (NTR) was expressed in the luminal cells of the mammary gland of transgenic mice using the ovine beta-lactoglobulin promoter. Treatment of NTR expressing animals with the prodrug CB1954 (5-aziridin-1-yl-2-4-dinitrobenzamide) resulted in a rapid and selective killing of this population of cells whereas the closely associated myoepithelial cells were unaffected. NTR-mediated inducible cell ablation offers a number of advantages over the use of HSV1-tk for the selective killing of cells in vivo.
authors.affiliation: Division of Molecular Biology, Roslin Institute, Roslin, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Clark
authors.forename: M
authors.initials: M
authors.lastname: Iwobi
authors.forename: W
authors.initials: W
authors.lastname: Cui
authors.forename: M
authors.initials: M
authors.lastname: Crompton
authors.forename: G
authors.initials: G
authors.lastname: Harold
authors.forename: S
authors.initials: S
authors.lastname: Hobbs
authors.forename: T
authors.initials: T
authors.lastname: Kamalati
authors.forename: R
authors.initials: R
authors.lastname: Knox
authors.forename: C
authors.initials: C
authors.lastname: Neil
authors.forename: F
authors.initials: F
authors.lastname: Yull
authors.forename: B
authors.initials: B
authors.lastname: Gusterson
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001388:Aziridines / Q000494:pharmacology*; D018929:Cell Culture Techniques; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005260:Female; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D007124:Immunoenzyme Techniques; D007774:Lactation; D007782:Lactoglobulins / Q000235:genetics; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008822:Mice, Transgenic; D009601:Nitroreductases / Q000235:genetics* / Q000378:metabolism; D011355:Prodrugs / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D001388:Aziridines; D007782:Lactoglobulins; D011355:Prodrugs; C100099:tretazicar; D009601:Nitroreductases