title: Proliferation cell nuclear antigen (PCNA) expression in cervical intraepithelial neoplasia (CIN). An immunohistochemical study.
abstract: We analyzed the expression of proliferating cell nuclear antigen (PCNA) in ectocervical sections fixed in formalin and embedded in paraffin using monoclonal antibody PC10, PAP and APAAP methods. A total of 156 cases was studied: 32 cases with normal epithelium, 33 cases with CIN I, 36 cases with CIN II, 55 cases with CIN III. We evaluated: 1. the index of PCNA (IPCNA), i.e. the proportion of PCNA-positive cells relative to all cells in the epithelium; 2. the IPCNA in each epithelial layer; 3. the epithelial height with PCNA-positive cells relative to the total height of the epithelium; 4. changes in the IPCNA with respect to the presence or absence of histological signs of HPV infection. We found: 1. a significant correlation between the index of PCNA and the severity of dysplasia in the whole epithelium and its individual layers; 2. a significant correlation between the epithelial height with PCNA-positive cells relative to the total height of the epithelium and the severity of dysplasia; 3. a lack of correlation between the IPCNA and HPV(+) and HPV(-) cases.
authors.affiliation: Department of Pathomorphology, Faculty of Medicine, Pomeranian Medical School, Szczecin.
authors.forename: M
authors.initials: M
authors.lastname: Smela
authors.forename: M
authors.initials: M
authors.lastname: Chosia
authors.forename: W
authors.initials: W
authors.lastname: Domagała
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D002583:Uterine Cervical Neoplasms / Q000276:immunology*; D002578:Uterine Cervical Dysplasia / Q000276:immunology*
chemical_list: D018809:Proliferating Cell Nuclear Antigen