title: ABO genotyping by PCR-direct sequencing.
abstract: The PCR-direct sequence method was applied to ABO genotyping. At the 261st nucleotide of the genes of A and B glycosyltrasferase, it was easily detected that the nucleotide was guanine in AA, AB and BB genotypes and that the nucleotide was ademine in only OO. In AO and BO, substitution of A to G was confirmed by the dye primer method, but it was difficult to detect correctly by the dye terminator method. At the 297th, nucleotide substitution between A and B alleles was confirmed by the both methods. As this position, O allele was subdivided into three types, OAOA, OGOG and OAOG. At the 703rd, nucleotide substitution between A and B alleles was easily detected by the both methods. The PCR-direct sequence method was suitable to confirm the nucleotide substitution or deletion directly and to prevent the mistyping by other methods.
authors.affiliation: Department of Forensic Medicine, Tohoku University, School of Medicine, Sendai.
authors.forename: M
authors.initials: M
authors.lastname: Nata
authors.forename: J
authors.initials: J
authors.lastname: Kanetake
authors.forename: N
authors.initials: N
authors.lastname: Adachi
authors.forename: M
authors.initials: M
authors.lastname: Hashiyada
authors.forename: Y
authors.initials: Y
authors.lastname: Aoki
authors.forename: K
authors.initials: K
authors.lastname: Sagisaka
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D005838:Genotype; D016695:Glycosyltransferases / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000017:ABO Blood-Group System; D016695:Glycosyltransferases