title: Morphological analysis of renal cell culture models of calcium phosphate stone formation.
abstract: Cell culture models of calcium phosphate renal stone formation were established using the MDCK cell line. Renal microliths were detected within pseudocysts in three-dimensional soft agar cultures, and were also observed in the basal region of cells lining the cell sheet, and immediately beneath domes or blisters in monolayers and collagen gel cultures. Light and scanning electron microscopy indicated that these microliths had a similar lamellated and spherical appearance to those in humans. These microliths were first detected microscopically after 21 days of culture, and were found to be composed of calcium phosphate by X-ray and micro-infrared spectroscopic analyses. These culture models may provide a powerful new tool to study the pathogenesis of renal stone diseases and/or calcium phosphate stone formation in humans and animals.
authors.affiliation: First Department of Pathology, Hamamatsu University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Naito
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtawara
authors.forename: S
authors.initials: S
authors.lastname: Kageyama
authors.forename: M
authors.initials: M
authors.lastname: Nakano
authors.forename: A
authors.initials: A
authors.lastname: Ichiyama
authors.forename: M
authors.initials: M
authors.lastname: Fujita
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Kawabe
authors.forename: I
authors.initials: I
authors.lastname: Kino
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D006801:Humans; D007668:Kidney / Q000473:pathology* / Q000648:ultrastructure; D007669:Kidney Calculi / Q000737:chemistry* / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D008954:Models, Biological; D013055:Spectrophotometry, Infrared
chemical_list: D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous