title: Virulence patterns from septicemic Escherichia coli O78 strains.
abstract: Several septicemic Escherichia coli O78 strains, isolated from different sources, were characterized phenotypically and genotypically. Two avian isolates, one of which is known to carry the AC/I fimbriae, hybridized with the sfa determinant in colony dot-blot assay. Southern hybridizations with specific sfa probes, following pulsed-field gel electrophoresis (PFGE), showed positive hybridization to the same fragment in each of these strains. Determination of the N-terminal amino acid sequence of the AC/I major subunit gene revealed high similarity to the sequence of the SfaA-II protein. These data suggest that the adhesin gene cluster, coding for AC/I fimbriae, belongs to the S-fimbrial adhesin family.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Babai
authors.forename: G
authors.initials: G
authors.lastname: Blum-Oehler
authors.forename: B E
authors.initials: BE
authors.lastname: Stern
authors.forename: J
authors.initials: J
authors.lastname: Hacker
authors.forename: E Z
authors.initials: EZ
authors.lastname: Ron
mesh_terms: D018829:Adhesins, Bacterial / Q000032:analysis / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D016667:Bacterial Capsules / Q000737:chemistry / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D015139:Blotting, Southern; D002645:Chickens; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D006877:Hydroxamic Acids / Q000032:analysis / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D015183:Restriction Mapping; D018805:Sepsis / Q000382:microbiology*; D012703:Serotyping; D012756:Sheep; D017262:Siderophores / Q000032:analysis / Q000235:genetics; D014422:Turkeys; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D006877:Hydroxamic Acids; D017262:Siderophores; C031819:aerobactin