Micronucleus induction by neocarzinostatin and methyl methanesulfonate in ionizing radiation--sensitive Chinese hamster V79 cell mutants.
Two mutant V79 Chinese hamster cell lines (V-E5, XR-V15B) which show hypersensitivities to DNA damage and their two parental cell lines (V79-LE, V79-B) were used for micronucleus studies. The characteristics of V-E5 strongly resemble those of cells derived from patients suffering from the genomic instability syndrome ataxia telangiectasia, whereas XR-V15B has a decreased ability to rejoin double-strand breaks. The two cell lines V-E5 and XR-V15B showed increased spontaneous micronucleus frequencies and higher sensitivity for micronucleus induction by methyl methanesulfonate (MMS) and neocarzinostatin (NCS) both with and without the use of cytochalasin B in the micronucleus assay. Thus, defects in cellular responses to DNA damage are modulating factors in micronucleus formation.
Stopper
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H
Department of Toxicology, University of Würzburg, Germany. stopper@toxi.uni-wuerzburg.de
Full
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Helbig
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Speit
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G
D000818:Animals; D001260:Ataxia Telangiectasia / Q000473:pathology; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D003571:Cytochalasin B / Q000494:pharmacology; D004249:DNA Damage; D004260:DNA Repair; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D019384:Nucleic Acid Synthesis Inhibitors / Q000633:toxicity*; D011839:Radiation, Ionizing; D009353:Zinostatin / Q000633:toxicity*
D009153:Mutagens; D019384:Nucleic Acid Synthesis Inhibitors; D003571:Cytochalasin B; D009353:Zinostatin; D008741:Methyl Methanesulfonate