A variation of the tissue print technique for studying isolated spinal cord cells in situ.
We present a novel variation of the tissue print technique which yields isolated spinal cells that retain their original topographical organization in situ. A light papain digestion combined with a low-melting-point agarose as the adhesive substrate is used to "print' a monolayer of cells from spinal cord slices. Printed cells are viable for studies using electrophysiological approaches. This technique provides a means to bridge the gap between properties of isolated cells and their topographical identity in spinal cord.
MacDonald
S C
SC
Department of Physiology, University of Manitoba, Winnipeg, Canada.
Hochman
S
S
D000818:Animals; D000831:Animals, Newborn; D002469:Cell Separation; D003584:Cytological Techniques; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology* / Q000502:physiology*