Identification of a Shigella flexneri criR gene increasing ipa genes expression: a novel member of response regulators of the two-component signal transduction family.
A genetic locus named cri, which enhanced the expression of ipa genes, was cloned into Escherichia coli K-12 from Shigella flexneri 1b chromosomal DNA. Subcloning and Tn5-Tc1 transposon experiments showed that cri locus was located on a 2.6-kb HindIII fragment. Nucleotide sequence analysis of the region revealed at least three open reading frames (ORF), one of which, named criR, encoded a protein of 226 amino-acid residues and transcriptionally increased the ipaB expression. The deduced regulatory protein CriR shared a significant homology with bacterial transcriptional activators of the two-component signal transduction family. A homologue of the criR gene was present in genomic DNA of Shigella spp. and E. coli strains, and mapped at the 14.6-min region of E. coli K-12 chromosomal DNA. These results indicate that criR is a new member of response regulators.
Qi
M S
MS
Department of Bacteriology, National Institute of Health, Toyama.
Yoshikura
H
H
Watanabe
H
H
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics*; D002874:Chromosome Mapping; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D015698:Genomic Library; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012763:Shigella flexneri / Q000235:genetics* / Q000472:pathogenicity; D015398:Signal Transduction / Q000235:genetics; D013045:Species Specificity; D014157:Transcription Factors; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C106728:CriR protein, Shigella flexneri; D014157:Transcription Factors; C087447:ipaB protein, Shigella