Expression of adhesion molecules and activation markers on lymphocytes and monocytes during hemodialysis.
Patients with end-stage renal failure undergoing chronic hemodialysis manifest alterations in cell-mediated immune response despite adequate urea clearance. We conducted a clinical trial in 8 patients (6 male, 2 female; 57.6 +/- 19.0 years) to investigate cell subsets and adhesion molecules on mononuclear blood cells during hemodialysis in patients on either cuprophane (n = 60 or polysulfone (n = 2). Cells were analyzed from the arterial line before, at 5, 10, 15, 30, 90 and 240 min with a panel of 33 surface markers including antibodies towards CD3, CD4, TCR-alpha/beta, CD7, CD8, CD11a-c, CD14, CD18, CD19, CD25, CD28, CD29, CD38, CD44, CD49a-e, CD51/61, CD54, CD56, CD58, CD62, CD106, and HLA class II using single fluorescence analysis. In vivo results were compared with in vitro tests. Our results suggest a substantial difference in the expression of various surface markers and cell adhesion molecules (lymphocytes: CD4, CD25, CD28, CD51, CD54; monocytes: CD49, CD54, CD58, CD62E, CD62P) compared to healthy persons, and a distinct modulation during hemodialysis (lymphocytes: CD11, CD18, CD49, CD51, CD61; monocytes: CD29, CD49, CD51, CD54, CD61) which might affect the immune response beyond the single dialysis session.
Braun
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Medizinische Universitätsklinik und Poliklinik, Tübingen, Germany.
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D006435:Renal Dialysis
D015703:Antigens, CD; D015815:Cell Adhesion Molecules