Induction of renin in medial smooth muscle cells by balloon injury.
We studied the expression of each component of the renin-angiotensin system (renin, angiotensin I-converting enzyme, angiotensinogen, and angiotensin II type I receptor) in balloon-injured rat carotid artery. We assessed the expression levels of the respective mRNAs by competitive polymerase chain reaction. Renin mRNA concentration was markedly increased 24 hours after balloon injury and remained higher than that in the control at 7 days after balloon injury. Angiotensin-converting enzyme mRNA concentration was decreased 24 hours after balloon injury and was increased at 14 days after balloon injury. No significant change in angiotensinogen mRNA concentration was observed throughout the study period. Angiotensin type I receptor mRNA concentration was increased beginning 3 days after balloon injury and remained higher than that in the control at 14 days after balloon injury. Immunohistochemical analysis showed that renin was transiently expressed in medial smooth muscle cells after balloon injury. Administration of quinapril markedly reduced neointimal formation and was accompanied by an attenuation of the increase in the concentrations of angiotensin type I receptor and angiotensin-converting enzyme mRNAs. The upregulation of renin mRNA in balloon-injured rat carotid artery preceded and may play a role in neointimal formation.
Iwai
N
N
1st Department of Internal Medicine, Shiga University of Medical Sciences, Ohtsu City, Japan. iwai@suncuore.shiga-med.ac.jp
Izumi
M
M
Inagami
T
T
Kinoshita
M
M
D000704:Analysis of Variance; D000803:Angiotensin I / Q000235:genetics; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000808:Angiotensinogen / Q000235:genetics; D000818:Animals; D020212:Carotid Artery Injuries; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000166:cytology* / Q000293:injuries; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D016133:Polymerase Chain Reaction; D000077583:Quinapril; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011945:Receptors, Angiotensin / Q000235:genetics; D012083:Renin / Q000235:genetics*; D044005:Tetrahydroisoquinolines; D013997:Time Factors; D017540:Tunica Media / Q000166:cytology* / Q000187:drug effects / Q000293:injuries; D015854:Up-Regulation
D000806:Angiotensin-Converting Enzyme Inhibitors; D007546:Isoquinolines; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D044005:Tetrahydroisoquinolines; D000808:Angiotensinogen; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A; D012083:Renin; D000077583:Quinapril