title: Effects of chlorhexidine on the bacterial colonization and degradation of dentin and completely demineralized dentin in situ.
abstract: The effects of 0.2% chlorhexidine on selected plaque microorganisms were studied in an intraoral dentin caries model. In 8 individuals wearing partial dentures, sound and completely demineralized dentin specimens were placed consecutively in 2 periods of 4 weeks, respectively. Throughout the experimental period, the specimens were treated 2 x daily with 0.2% chlorhexidine; control specimens were treated with water. Plaque accumulation on the specimens was left undisturbed. No protection against demineralization of the dentin or degradation of the dentin collagen by the chlorhexidine treatment was observed. The chlorhexidine treatment did not result in a reduction of the total cultivable flora when compared with the control specimens. A significant reduction of mutans streptococci and total streptococci recovered from completely demineralized dentin treated with chlorhexidine was observed, but the proportions of Actinomyces and lactobacilli were not affected significantly. It is speculated that areas of exposed roots, which are difficult to reach by oral hygiene measurements, such as approximal surfaces, will not be protected by a 0.2% chlorhexidine mouthrinse against the caries process.
authors.affiliation: Department of Cariology, Academic Centre for Dentistry Amsterdam (ACTA), The Netherlands. A.van.strijp@acta.nl
authors.forename: A J
authors.initials: AJ
authors.lastname: van Strijp
authors.forename: T J
authors.initials: TJ
authors.lastname: van Steenbergen
authors.forename: J M
authors.initials: JM
authors.lastname: ten Cate
mesh_terms: D000190:Actinomyces / Q000187:drug effects / Q000254:growth & development; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D002417:Cattle; D002710:Chlorhexidine / Q000627:therapeutic use*; D003094:Collagen / Q000032:analysis; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology; D003804:Dentin / Q000737:chemistry / Q000187:drug effects / Q000382:microbiology*; D003829:Denture, Partial; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008852:Microradiography; D008875:Middle Aged; D009067:Mouthwashes; D009910:Oral Hygiene; D010919:Placebos; D017213:Root Caries / Q000382:microbiology; D013291:Streptococcus / Q000187:drug effects / Q000254:growth & development; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development; D017001:Tooth Demineralization / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000891:Anti-Infective Agents, Local; D009067:Mouthwashes; D010919:Placebos; D003094:Collagen; D002710:Chlorhexidine