Interruption of the phosphoglucose isomerase gene results in glucose auxotrophy in Mycobacterium smegmatis.
Two glycerol utilization mutants of Mycobacterium smegmatis that were unable to utilize most carbon sources except glucose were isolated. Supplementation of these media with small amounts of glucose restored growth in the mutants; these strains are therefore glucose auxotrophs. The mutant phenotype is complemented by the gene encoding phosphoglucose isomerase (pgi), and direct measurement of enzyme activities in the mutants suggests that this gene product is absent in the auxotrophic strains. Mapping of the mutant allele by Southern analysis demonstrates the presence of a 1-kb deletion extending into the coding sequence of pgi. The possible roles of phosphoglucose isomerase in mycobacterial cell wall synthesis and metabolic regulation are discussed.
Tuckman
D
D
Department of Microbiology and Immunology, Albert Einstein Medical School, Bronx, New York, USA.
Donnelly
R J
RJ
Zhao
F X
FX
Jacobs
W R
WR
Connell
N D
ND
D000595:Amino Acid Sequence; D001483:Base Sequence; D002473:Cell Wall / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D005947:Glucose / Q000378:metabolism*; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics* / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose; D005990:Glycerol