A simple artificial urine for the growth of urinary pathogens.
A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10(8) cfu ml-1, as found in normal urine. Reproducible, steady-state growth also occurred over many generations in continuous culture. AUM was capable of forming crystals and encrustations resembling those found in natural urinary tract infections. The medium is a suitable replacement for normal urine for use in a wide range of experiments modelling the growth and attachment of urinary pathogens in the clinical environment.
Brooks
T
T
Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.
Keevil
C W
CW
D001431:Bacteriological Techniques; D003470:Culture Media / Q000032:analysis / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000254:growth & development*; D011513:Proteus mirabilis / Q000254:growth & development*; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D013212:Staphylococcus epidermidis / Q000254:growth & development*; D014510:Urease / Q000378:metabolism; D014556:Urine / Q000737:chemistry*
D003470:Culture Media; D014510:Urease