Genomic organization of the mouse adrenocorticotropin receptor.
As a step toward understanding the transcriptional regulation of the adrenocorticotropin receptor (ACTH-R) gene, we examined the full length cDNA sequence of the mouse ACTH-R by rapid amplification of cDNA ends, and the organization of the gene. Mouse ACTH-R mRNA consists of 374 bp in the 5'-untranslated region (UTR), 888 bp in the coding sequence, and 445 bp in the 3'-UTR, the 1707 bp being fairly compatible with the 1.8-kb adrenal mRNA detected by Northern analysis. The mouse ACTH-R gene consists of at least four exons; the first three exons encode 5'-UTR and the fourth exon encodes part of 5'-UTR, the entire coding region, and the whole of 3'-UTR. We also defined two mRNA species, one with and one without the 57-bp exon 2, produced by alternative splicing.
Shimizu
C
C
Department of Medicine II, Hokkaido University School of Medicine, Sapporo, Japan.
Kubo
M
M
Saeki
T
T
Matsumura
T
T
Ishizuka
T
T
Kijima
H
H
Kakinuma
M
M
Koike
T
T
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018076:DNA, Complementary; D005091:Exons; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D018043:Receptors, Corticotropin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D018043:Receptors, Corticotropin; D000324:Adrenocorticotropic Hormone