Osmium ammine-B and electron spectroscopic imaging of ribonucleoproteins: correlation of stain and phosphorus.
Ultra-thin sections of Chironomus salivary glands were stained in a non-Feulgen procedure with osmium ammine-B and imaged at several electron energy-loss windows. For two types of RNP-containing structures (ie Balbiani ring granules and endoplasmic reticulum), a significant spatial correlation was observed between stain distribution and net phosphorus distribution. Non-Feulgen osmium ammine-B staining does not require the use of ultra-thin sections and can approximate the distribution of nucleic acid phosphorus.
Olins
A L
AL
University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge National Laboratory 37831-8080, USA.
Olins
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DE
Bazett-Jones
D P
DP
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D002683:Chironomidae; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D008854:Microscopy, Electron / Q000379:methods*; D017892:Osmium Compounds; D010758:Phosphorus / Q000032:analysis*; D000644:Quaternary Ammonium Compounds; D016601:RNA-Binding Proteins / Q000032:analysis; D012261:Ribonucleoproteins / Q000032:analysis* / Q000648:ultrastructure; D012270:Ribosomes / Q000737:chemistry / Q000648:ultrastructure; D012469:Salivary Glands / Q000737:chemistry / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods
D034441:Heterogeneous-Nuclear Ribonucleoproteins; D017892:Osmium Compounds; D000644:Quaternary Ammonium Compounds; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C076686:osmium ammine; D010758:Phosphorus