title: The determination of the in situ structure by nuclear spin contrast variation.
abstract: Polarized neutron scattering from polarized nuclear spins in hydrogenous substances opens a new way of contrast variation. The enhanced contrast due to proton spin polarization was used for the in situ structure determination of tRNA of the functional complex of the E. coli ribosome.
authors.affiliation: GKSS Forschungszentrum, Geesthacht, Germany.
authors.forename: H B
authors.initials: HB
authors.lastname: Stuhrmann
authors.forename: K H
authors.initials: KH
authors.lastname: Nierhaus
mesh_terms: D003461:Crystallography / Q000295:instrumentation / Q000379:methods; D003903:Deuterium; D004926:Escherichia coli / Q000648:ultrastructure*; D008961:Models, Structural; D008962:Models, Theoretical; D009502:Neutrons; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000737:chemistry*; D012360:RNA, Transfer, Phe / Q000737:chemistry*; D012270:Ribosomes / Q000648:ultrastructure*; D012542:Scattering, Radiation
chemical_list: D012329:RNA, Bacterial; D012358:RNA, Transfer, Met; D012360:RNA, Transfer, Phe; D003903:Deuterium