Expression of an isoflavone reductase-like gene enhanced by pollen tube growth in pistils of Solanum tuberosum.
Successful sexual reproduction relies on gene products delivered by the pistil to create an environment suitable for pollen tube growth. These compounds are either produced before pollination or formed during the interactions between pistil and pollen tubes. Here we describe the pollination-enhanced expression of the cp100 gene in pistils of Solanum tuberosum. Temporal analysis of gene expression revealed an enhanced expression already one hour after pollination and lasts more than 72 h. Increase in expression also occurred after touching the stigma and was not restricted to the site of touch but spread into the style. The predicted CP100 protein shows similarity to leguminous isoflavone reductases (IFRs), but belongs to a family of IFR-like NAD(P)H-dependent oxidoreductases present in various plant species.
van Eldik
G J
GJ
Department of Experimental Botany, University of Nijmegen, Netherlands.
Ruiter
R K
RK
Colla
P H
PH
van Herpen
M M
MM
Schrauwen
J A
JA
Wullems
G J
GJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010812:Physical Stimulation; D018520:Plant Shoots / Q000201:enzymology*; D011058:Pollen / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics*; D015854:Up-Regulation
D018076:DNA, Complementary; D010088:Oxidoreductases; C071072:vestitone reductase; D044925:Oxidoreductases Acting on CH-CH Group Donors