[Changes in the activity level of tumor necrosis factor in the blood serum after irradiation and a combined radiation-thermal lesion].
To elucidate the role of the tumor-necrosis factor (TNF) in the pathogenesis of an additional aggravating effect of a thermal burn on the development and outcome of a radiation disease, the changes in the activity of cytokine in the blood serum of irradiated mice were studied. The mice were exposed to radiation at a dose of 7 Gy and subjected to a combined radiation-thermal injury (CRTI). The level of endogenous and LPS-induced TNF was determined in a cytotoxic test on a mice fibroblast L-929 culture. It was found that the activity of TNF in the blood circulation of mice subjected to irradiation and CRTI did not increase much. As a result of an intravenous injection of a provoking stimulus (LPS from E. coli), an increase in the TNF activity in the early period after irradiation was higher than with CRTI. There is no correlation between the increase in the death rate for mice with a combined injury and the changes in the LPS-induced TNF activity.
Petrov
V N
VN
Budagov
R S
RS
D000818:Animals; D002056:Burns / Q000097:blood*; D004926:Escherichia coli; D005720:Gamma Rays; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011833:Radiation Injuries, Experimental / Q000097:blood*; D018709:Statistics, Nonparametric; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000187:drug effects / Q000528:radiation effects*; D014916:Whole-Body Irradiation
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha
Izmeneniia urovnia aktivnosti tumornekroticheskogo faktora v syvorotke krovi posle oblucheniia i kombinirovannogo radiatsionno-termicheskogo porazheniia.