title: Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area.
abstract: We investigated extracts of timothy grass pollen from four seasons (1989, 1990, 1991, and 1994) by protein content, SDS-PAGE, immunoblot, RAST, RAST inhibition, and crossed immunoelectrophoresis. Extract of the pollen from 1991 showed the lowest yield in quantitative assays. SDS-PAGE, crossed immunoelectrophoresis, RAST, and RAST inhibition expressed approximately comparable patterns for all extracts except that from 1991. Obviously, the quality of grass pollens, as shown for some ragweed (Ambrosia elatior) pollens depend on year of collection. Our findings are partially in agreement with some earlier examinations of the quality of timothy pollen from different pollen seasons.
authors.affiliation: Faculty of Chemistry, Belgrade, Yugoslavia.
authors.forename: M D
authors.initials: MD
authors.lastname: Gavrović
authors.forename: T
authors.initials: T
authors.lastname: Trtić
authors.forename: Z
authors.initials: Z
authors.lastname: Vujcić
authors.forename: S
authors.initials: S
authors.lastname: Petrović
authors.forename: R M
authors.initials: RM
authors.lastname: Jankov
mesh_terms: D000485:Allergens / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007123:Immunoelectrophoresis, Two-Dimensional; D008853:Microscopy; D010936:Plant Extracts / Q000737:chemistry / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D011852:Radioallergosorbent Test; D012621:Seasons; D015022:Yugoslavia
chemical_list: D000485:Allergens; D010936:Plant Extracts; D010940:Plant Proteins