title: A glutathione S-transferase gene of the vector mosquito, Anopheles gambiae.
abstract: A cDNA (D7-8B) 1456 nucleotides in length was isolated from an adult, female-specific Anopheles gambiae library and identified as a member of the glutathione S-transferase (GST) gene family by virtue of the inferred amino acid sequence. The gene, AgGST2-1, specifies a protein that is 57% identical to the Drosophila melanogaster gene, DmGST-2. The conceptual translation product also shows similarity to the pi family of vertebrate GSTs. Northern analysis reveals multiple and abundant transcription products in larval, pupal and adult RNA preparations. Southern analyses of genomic DNA and hybridizations in situ to polytene chromosomes both suggest that there are multiple members of the GST gene family in Anopheles gambiae.
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California at Irvine, 92717, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Reiss
authors.forename: A A
authors.initials: AA
authors.lastname: James
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018076:DNA, Complementary; D005260:Female; D017344:Genes, Insect; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics*; D007303:Insect Vectors / Q000201:enzymology* / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D005982:Glutathione Transferase