[The determination of the content of the cholera toxin gene in the composition of the DNA from Vibrio cholerae strains by means of the nested polymerase chain reaction].
The possibility of detecting cholera toxin genes in V.cholerae enterotoxigenic strains by the method of "nested" polymerase chain reaction with the use of primers on the DNA area of operon ctx of AB genes. The possibility of the detection of several V.cholerae cells by this method was shown with the use of a series of bacterial lysate dilutions. The newly developed test system for the detection of cholera toxin gene on the basis of the analysis of bacterial lysates of V.cholerae nontoxigenic strains, as well as Escherichia coli toxigenic and nontoxigenic strains, was shown to be highly specific.
Glukhov
A I
AI
Onishchenko
G G
GG
Gordeev
S A
SA
Grachev
S V
SV
Veriasov
V N
VN
Severin
E S
ES
Popov
Iu A
IuA
Kulichenko
A N
AN
Naumov
A V
AV
D001483:Base Sequence; D002772:Cholera Toxin / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial; D002772:Cholera Toxin
Opredelenie soderzhaniia gena kholernogo toksina v sostave DNK iz shtammov Vibrio cholerae metodom "gnezdovoĭ" polimeraznoĭ tsepnoĭ reaktsii.