Monitoring of polychlorinated biphenyls in human blood plasma: methodological developments and influence of age, lactation, and fish consumption.
Human plasma samples from 50 wives of fishermen have been analyzed with respect to PCBs. The non-ortho-substituted PCB congeners CB-126 and CB-169 were determined by mass spectrometry in negative ion chemical ionization mode, which demonstrated a limit of detection of 30 fg. The recoveries of the internal standards used for determination of ortho-substituted CBs were approximately 95%. Two methods, one gravimetric and the other based on enzymatic determinations of triglycerides, cholesterol and phospholipids, were compared for the determination of total amount of lipids in the plasma samples; the correlation coefficient was 0.82 and the slope 0.98. For practical reasons, enzymatic determinations are recommended for further use. The total, lipid-adjusted concentrations of PCBs in plasma were influenced by age, total lactation time and consumption of fatty fish from the Baltic Sea.
Grimvall
E
E
Division of Analytical Chemistry, National Institute for Working Life, S-171 84 Solna, Sweden.
Rylander
L
L
Nilsson-Ehle
P
P
Nilsson
U
U
Strömberg
U
U
Hagmar
L
L
Ostman
C
C
D000328:Adult; D000367:Age Factors; D000818:Animals; D004032:Diet; D005260:Female; D005399:Fishes; D006801:Humans; D007774:Lactation / Q000097:blood*; D008875:Middle Aged; D011078:Polychlorinated Biphenyls / Q000097:blood*
D011078:Polychlorinated Biphenyls