title: Sodium azide induces mitotic recombination in Drosophila melanogaster larvae.
abstract: Sodium azide (NaN3), a potent mutagen for bacteria and barley, was tested for somatic mutation and mitotic recombination induction in wing imaginal disc cells of Drosophila melanogaster. Comparisons were made among inversion-free flr3/mwh, inversion-heterozygous TM3, Ser/mwh, and inversion-free, high bioactivation OR(R), flr3/mwh flies. Third instar larvae were exposed chronically for 48 h to sodium azide at 0.5, 0.63, 0.75, 0.88 and 1.0 mM. The frequencies of spots per wing obtained in the three kinds of progeny scored were compared. In inversion-free flies, sodium azide induced large single and total spots at all concentrations tested, and small single and twin spots at 0.75 mM and higher concentrations. In contrast, it failed to increase the frequency of small and large single spots in inversion-heterozygous flies. In high bioactivation flies (which are inversion-free), sodium azide increased the frequency of large single spots at 0.63, 0.88 and 1.0 mM and the frequency of total spots at 0.63 mM. From the absence of genotoxic activity observed in inversion-heterozygous flies it is concluded that sodium azide induces exclusively mitotic recombination in wing somatic cells of Drosophila melanogaster larvae after chronic exposure. This recombinogenic activity is reduced in the presence of high bioactivation capacity.
authors.affiliation: Laboratorio de Genética, Facultad de Ciencias, Universidad Nacional Autónoma de México, Coyoacan, México D.F., México.
authors.forename: E
authors.initials: E
authors.lastname: González-César
authors.forename: P
authors.initials: P
authors.lastname: Ramos-Morales
mesh_terms: D000818:Animals; D001386:Azides / Q000494:pharmacology*; D007446:Chromosome Inversion; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D005260:Female; D006579:Heterozygote; D007814:Larva; D008297:Male; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens / Q000494:pharmacology*; D010641:Phenotype; D011995:Recombination, Genetic / Q000187:drug effects*; D012044:Regression Analysis; D019810:Sodium Azide; D012984:Software; D018709:Statistics, Nonparametric; D014921:Wings, Animal
chemical_list: D001386:Azides; D009153:Mutagens; D019810:Sodium Azide