title: The effect of increased egg consumption on plasma cholesteryl ester transfer activity in healthy subjects.
abstract: BACKGROUND
  Plasma cholesteryl ester transfer activity influences the distribution of cholesterol among plasma lipoproteins and changes in its activities may influence the response of plasma lipoprotein cholesterol levels to increased cholesterol intake in the form of eggs.
  
  
  OBJECTIVE
  The aim of the study was to determine the effect of increased egg intake on plasma newly synthesized cholesteryl ester transfer (NCET) activity and plasma lipids and lipoprotein levels in free-living men and women.
  
  
  METHODS
  The study was longitudinal and included a 12 d intervention arm and a similar control period immediately prior to the intervention to account for any changes in variables with time.
  
  
  METHODS
  Thirty two healthy subjects aged 20-57 y and consuming self-selected diets were recruited. Twelve men and 14 women were entered into the intervention arm of the study. Twelve of these subjects and six others were in the control group.
  
  
  METHODS
  Subjects who participated in the intervention phase received one additional egg per day.
  
  
  RESULTS
  Plasma NCET activity decreased significantly (P = 0.05) in the 26 subjects who consumed an extra egg, mainly due to a significant (P = 0.02) decrease (-13.4%) in the activity in the women. Plasma cholesterol and apolipoprotein B-containing lipoprotein cholesterol increased significantly (P < 0.05) in the intervention group and in men (P = 0.02) but not in women. Age was correlated significantly (r = 0.519, P < 0.01) with the change in plasma NCET activity in all subjects and in men (r = -0.644, P < 0.05). The decrease in plasma NCET activity was significantly (P = 0.02) less in the lowest tertile compared with other tertiles of self-reported habitual dietary cholesterol intake independent of age and gender.
  
  
  CONCLUSIONS
  These data suggest that an increase in dietary cholesterol reduces plasma NCET activity particularly in women and older men and individuals with higher habitual intakes of cholesterol. Furthermore, a reduction in plasma NCET activity may tend to limit the increase in apoB-containing lipoprotein cholesterol in women during increased egg consumption.
authors.affiliation: Department of Medicine, University of Otago Medical School, Dunedin, New Zealand.
authors.forename: W H
authors.initials: WH
authors.lastname: Sutherland
authors.forename: M J
authors.initials: MJ
authors.lastname: Ball
authors.forename: H
authors.initials: H
authors.lastname: Walker
mesh_terms: D000328:Adult; D015992:Body Mass Index; D002352:Carrier Proteins / Q000097:blood / Q000187:drug effects* / Q000378:metabolism*; D053480:Cholesterol Ester Transfer Proteins; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000494:pharmacology*; D008078:Cholesterol, LDL / Q000097:blood; D004531:Eggs; D005260:Female; D006023:Glycoproteins; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D002791:Cholesterol, Dietary; D008078:Cholesterol, LDL; D006023:Glycoproteins