[Value of carbon in the thrombogenicity of cardiovascular prostheses].
Forty five Carbon-lined (CL) and 45 Standard (ST) 4 mm internal diameter polytetrafluoroethylene (PTFE) grafts were implanted as aortic interpositions in 90 New Zealand rabbits. In a pilot study of 20 animals, 10 CL and 10 ST grafts were used to develop microsurgical techniques, then 60 grafts were placed in 60 rabbits with lower morbidity. The 2-hour graft patency (Doppler study, Transonic flow probe and 8 mHZ) showed a better patency rate in the CL group (93% versus 80%). In 10 animals, platelet accumulation was investigated in vivo using scintigraphy after injection of autologous platelets labeled with Indium111. In vitro, radioactivity counting of the explanted midgrafts sections at 2 hours revealed a 6-fold higher activity in ST grafts (6.60 +/- 1.98 x 103 platelets/mm2 versus 0.82 +/- 0.25 x 103 platelets/mm2; p < 0.05). Light microscopy found platelet and fibrin deposition (PFD) in nearly all ST grafts, whereas PFD were found in only 13% of the CL grafts corresponding to those thrombosed grafts (chi2:61.117; p < 0.001). Carbon-lining decreases platelet accumulation on PTFE grafts and the rabbit aortic interposition appears to constitute a quantitative and reproducible model for investigation of antithrombotic drugs.
Babatasi
G
G
Laboratoire de Thrombose Expérimentale, Institut des Cordeliers, Université P.M. Curie, Paris VI.
Bara
L
L
Massetti
M
M
Galateau
F
F
Bloch
M F
MF
Boulakia-Cohen
F
F
Khayat
A
A
Samama
M M
MM
D000818:Animals; D001792:Blood Platelets; D001807:Blood Vessel Prosthesis; D002244:Carbon; D006083:Graft Occlusion, Vascular / Q000097:blood / Q000000981:diagnostic imaging / Q000188:drug therapy; D007205:Indium Radioisotopes; D008866:Microsurgery; D011138:Polytetrafluoroethylene; D011183:Postoperative Complications; D011817:Rabbits; D011877:Radionuclide Imaging; D013927:Thrombosis / Q000097:blood* / Q000000981:diagnostic imaging / Q000188:drug therapy; D013997:Time Factors
D007205:Indium Radioisotopes; D002244:Carbon; D011138:Polytetrafluoroethylene
Intérêt du carbone dans la thrombogénicité des prothèses cardiovasculaires.