mRNA expression of the four isoforms of the human plasma membrane Ca(2+)-ATPase in the human hippocampus.
Ca2+ dyshomeostasis is a contributing factor to the development and progression of neurodegenerative disease. Plasma membrane Ca(2+)-ATPases (PMCAs) are responsible for setting intracellular Ca2+ levels and may be involved in the dynamic processing of Ca2+ loads in normal and pathological conditions. In situ hybridization was employed to determine the expression pattern of the four human PMCA isoforms in the human hippocampus. PMCA1 and 3 mRNAs were weakly expressed throughout the hippocampal formation, whereas PMCA2 and 4 mRNA expression showed distinct regional differences, with increased levels in CA2 and the dentate gyrus. Differential expression of PMCA isoforms may reflect cellular differences in Ca(2+)-handling properties and provide a partial explanation for the differential susceptibility of hippocampal neurons to Ca(2+)-mediated cell death.
Zacharias
D A
DA
Department of Molecular Neuroscience, Mayo Graduate School, Mayo Clinic/Foundation, Rochester, MN 55905, USA.
DeMarco
S J
SJ
Strehler
E E
EE
D000252:Calcium-Transporting ATPases / Q000096:biosynthesis*; D002462:Cell Membrane / Q000201:enzymology; D017931:DNA Primers; D006624:Hippocampus / Q000201:enzymology*; D006801:Humans; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis*; D009474:Neurons / Q000201:enzymology; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D017966:Pyramidal Cells / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic
D017931:DNA Primers; D007527:Isoenzymes; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D000252:Calcium-Transporting ATPases