title: Immunohistochemical study of cathepsin G and medullasin in inflamed gingival tissues from periodontal patients.
abstract: Cathepsin G and medullasin are 2 major serine proteinases associated with the granular fraction of polymorphonuclear leukocytes (PMNs). To know their possible involvement in the pathophysiological gingival connective tissue turnover, we have determined the distribution and localization of these 2 enzymes in inflamed gingival tissues from periodontal patients by immunohistochemistry with discriminating antibodies specific for each enzyme. The gingival connective tissues were obtained from periodontitis patients with various inflammatory conditions and control healthy subjects without any clinical signs of periodontal inflammation. In all gingival specimens examined, cathepsin G and medullasin were found mainly in neutrophil-like cells and partly in macrophage-like cells. No positive staining for both enzymes was obtained in endothelial cells and fibroblasts in every part of the gingival tissues. Immunoreactivity for each enzyme in the gingival tissues from the periodontitis group was stronger and greater in the intensity and frequency than that from the control group and appeared to be increased with the severity of the disease In both groups, the number of immunoreactive cells for each enzyme was greater in the vicinity of pocket epithelium (zone I) than in the area of central connective tissue (zone II) or the area subjacent to the oral epithelium (zone III). While both enzymes in zones II and III were exclusively found in coarse granules, their stainings in zone I were not only coarse but also diffuse. These results strongly suggest that both enzymes may have some association with inflamed gingival tissue degradation.
authors.affiliation: Department of Periodontology, Nagasaki University School of Dentistry, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kunimatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Y
authors.initials: Y
authors.lastname: Hara
authors.forename: Y
authors.initials: Y
authors.lastname: Aoki
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: I
authors.initials: I
authors.lastname: Kato
mesh_terms: D000328:Adult; D000906:Antibodies; D056649:Cathepsin G; D002403:Cathepsins / Q000032:analysis*; D003238:Connective Tissue / Q000201:enzymology / Q000473:pathology; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000201:enzymology / Q000473:pathology; D004846:Epithelial Attachment / Q000201:enzymology / Q000473:pathology; D004848:Epithelium / Q000201:enzymology / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000473:pathology; D005881:Gingiva / Q000201:enzymology / Q000473:pathology; D005891:Gingivitis / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008264:Macrophages / Q000201:enzymology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology; D010514:Periodontal Pocket / Q000201:enzymology / Q000473:pathology; D010518:Periodontitis / Q000201:enzymology* / Q000473:pathology; D012697:Serine Endopeptidases / Q000032:analysis*
chemical_list: D000906:Antibodies; D002403:Cathepsins; D012697:Serine Endopeptidases; C034869:medullasin; C534094:CTSG protein, human; D056649:Cathepsin G