title: Serum succinate by capillary zone electrophoresis: marker candidate for hypoxia.
abstract: Serum succinate may offer an alternate analyte to lactate for the evaluation of hypoxia. To evaluate the potential uses of succinate, a relatively rapid capillary zone electrophoresis assay was developed for use in the clinical laboratory setting. Employing a simple indirect ultraviolet detection method with commercially available instrumentation, the limit of detection for serum succinate was determined to be 0.1 mumol/L, the upper limit of linearity 100 mumol/L, and the between-run coefficient of variation about 15 percent. Based on specimens from 202 apparently healthy adults, the non-parametric reference interval was 1.0 to 9.2 mumol/L. Preliminary studies in stored blood show succinate increased 2-fold while lactate increased 11-fold, suggesting that succinate may be a clinically useful marker for hypoxia in patients after blood transfusion. This assay provides a practical tool for the investigation of the clinical applications of succinate.
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132, USA.
authors.forename: G
authors.initials: G
authors.lastname: Komaromy-Hiller
authors.forename: P D
authors.initials: PD
authors.lastname: Sundquist
authors.forename: L J
authors.initials: LJ
authors.lastname: Jacobsen
authors.forename: K L
authors.initials: KL
authors.lastname: Nuttall
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D004355:Drug Stability; D004492:Edetic Acid; D019075:Electrophoresis, Capillary; D005260:Female; D006801:Humans; D000860:Hypoxia / Q000097:blood*; D019344:Lactic Acid / Q000097:blood; D008297:Male; D012016:Reference Values; D012680:Sensitivity and Specificity; D013386:Succinates / Q000097:blood*; D019802:Succinic Acid; D013696:Temperature
chemical_list: D015415:Biomarkers; D013386:Succinates; D019344:Lactic Acid; D004492:Edetic Acid; D019802:Succinic Acid