Incorporation of nisin in micro-particles of calcium alginate.
Nisin was successfully incorporated into a matrix of calcium alginate and ground into micro-particles smaller than 150 microns. Formation of micro-particles and incorporation of nisin was verified by scanning electron microscopy and by the reduction in the inactivation of nisin activity with proteolytic enzymes. Incorporation efficiency was 87-93% and the nisin in the alginate-incorporated form was 100% active against an indicator culture of Lactobacillus curvatus both in MRS broth and reconstituted skim milk.
Wan
J
J
Australian Food Industry Science Centre, Werribee, Victoria, Australia.
Gordon
J B
JB
Muirhead
K
K
Hickey
M W
MW
Coventry
M J
MJ
D000464:Alginates / Q000138:chemical synthesis* / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D001681:Biological Assay; D002918:Chymotrypsin / Q000494:pharmacology; D003470:Culture Media / Q000378:metabolism; D004339:Drug Compounding / Q000379:methods; D010450:Endopeptidases / Q000494:pharmacology; D005520:Food Preservatives / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008855:Microscopy, Electron, Scanning; D008892:Milk / Q000378:metabolism; D009561:Nisin / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology
D000464:Alginates; D003470:Culture Media; D005520:Food Preservatives; D009561:Nisin; D010450:Endopeptidases; D002918:Chymotrypsin