Effect of lipoproteins on the differentiation of 3T3-L1 and human preadipocytes in cell culture.
High-density and low-density lipoprotein (LDL) stimulated 3T3-L1 and human preadipocyte differentiation in vitro. In both cell types, LDL exhibited the greatest stimulatory effect. LDL suppressed the development of catecholamine-stimulated lipolysis in differentiating 3T3-L1 and human preadipocytes when present during preadipocyte proliferation and early stages of differentiation. The effect of lipoproteins on development of human preadipocyte (but not 3T3-L1) lipolysis may involve beta-adrenoceptors.
Stanton
L A
LA
Department of Biochemistry, University of Port Elizabeth, South Africa.
van de Venter
M
M
Litthauer
D
D
Oelofsen
W
W
D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects; D008074:Lipoproteins / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology; D051379:Mice; D011485:Protein Binding; D011943:Receptors, Adrenergic, beta / Q000378:metabolism
D002395:Catecholamines; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D011943:Receptors, Adrenergic, beta; D005954:Glucosephosphate Dehydrogenase; D007545:Isoproterenol; D005990:Glycerol