Monoclonal antibodies against Nalpha-(5'-phosphopyridoxyl)-L-lysine. Screening and spectrum of pyridoxal 5'-phosphate-dependent activities toward amino acids.
Cofactors may be expected to expand the range of reactions amenable to antibody-assisted catalysis. The biological importance of pyridoxal 5'-phosphate (PLP) as enzymic cofactor in amino acid metabolism and its catalytic versatility make it an attractive candidate for the generation of cofactor-dependent abzymes. Here we report an efficient procedure to screen antibodies for PLP-dependent catalytic activity and detail the spectrum of catalytic activities found in monoclonal antibodies elicited against Nalpha-(5'-phosphopyridoxyl)-L-lysine. This hapten is a nonplanar analog of the planar, resonance-stabilized coenzyme-substrate adducts formed in the PLP-dependent reactions of amino acids. The hapten-binding antibodies were screened for binding of the planar Schiff base formed from PLP and D- or L-norleucine by competition enzyme-linked immunosorbent assay. The Schiff base (external aldimine) is an obligatory intermediate in all PLP-dependent reactions of amino acids. This simple, yet highly discriminating screening step eliminated most of the total 24 hapten-binding antibodies. Three positive clones bound the Schiff base with L-norleucine, two preferred that with the D-enantiomer. The positive clones were assayed for catalysis of Schiff base formation and of the alpha,beta-elimination reaction with the D- and L-enantiomers of beta-chloroalanine. Three antibodies were found to accelerate aldimine formation, and two of these catalyzed the PLP-dependent alpha,beta-elimination reaction. One of the alpha, beta-elimination-positive antibodies catalyzed the transamination reaction with hydrophobic D-amino acids and oxoacids (Gramatikova, S. I., and Christen, P. (1996) J. Biol. Chem. 271, 30583-30586). All catalytically active antibodies displayed continuous turnover. No PLP-dependent reactions other than aldimine formation, alpha, beta-elimination of beta-chloroalanine and transamination were detected. The successive screening steps plausibly simulate the functional selection pressures having been operative in the molecular evolution of primordial PLP-dependent protein catalysts to reaction- and substrate-specific enzymes.
Gramatikova
S I
SI
Biochemisches Institut der Universität Zürich, CH-8057 Zürich, Switzerland.
Christen
P
P
D000345:Affinity Labels; D000596:Amino Acids / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000276:immunology; D004072:Digitonin / Q000378:metabolism; D007202:Indicators and Reagents / Q000378:metabolism; D007700:Kinetics; D008239:Lysine / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism; D008956:Models, Chemical; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000276:immunology / Q000378:metabolism*; D012663:Semicarbazides / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014633:Valine / Q000378:metabolism
C101404:6-N-(5'-phosphopyridoxyl)-2-N-acetyl-lysine methyl ester; D000345:Affinity Labels; D000596:Amino Acids; D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D012663:Semicarbazides; C042448:aldimine eliminator; D011732:Pyridoxal Phosphate; D014633:Valine; D008239:Lysine; D004072:Digitonin