Binding and uptake of 125iodine-labelled, oxidized low density lipoprotein by macrophages: comparison of the effects of alpha-tocopherol, probucol, pyridoxal-5'-phosphate and magnesium-pyridoxal-5'-phosphate-glutamate.
Specific and unspecific binding and uptake (internalization) by macrophages of 125iodine-labelled, copper-oxidized human low density lipoprotein is differently influenced by the anti-oxidants alpha-tocopherol (alpha-Toc), probucol (Prob), pyridoxal-5'-phosphate (PP) and the magnesium-pyridoxal-5'-phosphate glutamate complex (MPPG). Binding as well as internalization, mediated by the so-called "scavenger receptor" is lower in the presence of MPPG whereas both specific binding and internalization are enhanced. The comparison of the effects in vitro allows a rating of the potentially anti-atherogenic and thus protective effects of the tested substances as follows: MPPG > PP > alpha-Toc > Prob.
Selmer
D
D
Technische Universität München, Lehrstuhl für Phytopathologie, Freising-Weihenstephan.
Senekowitsch-Schmidtke
R
R
Schneider
W
W
Elstner
E F
EF
D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport / Q000187:drug effects; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D008565:Membrane Proteins; D009942:Organometallic Compounds / Q000494:pharmacology*; D011341:Probucol / Q000494:pharmacology*; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D011971:Receptors, Immunologic / Q000378:metabolism*; D011973:Receptors, LDL / Q000378:metabolism*; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000494:pharmacology*
D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D009942:Organometallic Compounds; D011971:Receptors, Immunologic; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; C099252:oxidized low density lipoprotein; D014810:Vitamin E; D011732:Pyridoxal Phosphate; D008274:Magnesium; D011341:Probucol