title: Isolation and characterization of a gene encoding alpha-tubulin from Candida albicans.
abstract: A gene encoding the alpha-tubulin of Candida albicans has been cloned and characterized. Nucleotide sequence analysis reveals the presence of an intron within the structural gene and predicts the synthesis of a polypeptide of 448 amino acid residues. Comparison of nucleotide and amino acid sequences with the Saccharomyces cerevisiae alpha-tubulin encoding genes shows a 75% homology and about 92% similarity respectively. In contrast to S. cerevisiae, C. albicans appears to possess only one gene for alpha-tubulin which is able to functionally complement a S. cerevisiae cold-sensitive tub1 mutant.
authors.affiliation: Department of Animal Biology and Genetics, University of Florence, Firenze, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Daly
authors.forename: A
authors.initials: A
authors.lastname: Yacoub
authors.forename: W
authors.initials: W
authors.lastname: Dundon
authors.forename: G
authors.initials: G
authors.lastname: Mastromei
authors.forename: K
authors.initials: K
authors.lastname: Islam
authors.forename: R
authors.initials: R
authors.lastname: Lorenzetti
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics*; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D018628:Gene Dosage; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014404:Tubulin / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D014404:Tubulin