Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells.
The proposed cis-Golgi vesicle receptor syntaxin 5 was found in a complex with Golgi-associated SNARE of 28 kDa (GOS-28), rbet1, rsly1, and two novel proteins characterized herein: rat sec22b and membrin, both cytoplasmically oriented integral membrane proteins. The complex appears to recapitulate vesicle docking interactions of proteins originating from distinct compartments, since syntaxin 5, rbet1, and GOS-28 localize to Golgi membranes, whereas mouse sec22b and membrin accumulate in the endoplasmic reticulum. Protein interactions in the complex are dramatically rearranged by N-ethylmaleimide-sensitive factor. The complex consists of two or more subcomplexes with some members (rat sec22b and syntaxin 5) in common and others (rbet1 and GOS-28) mutually exclusively associated. We propose that these protein interactions determine vesicle docking/fusion fidelity between the endoplasmic reticulum and Golgi.
Hay
J C
JC
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305-5428, USA.
Chao
D S
DS
Kuo
C S
CS
Scheller
R H
RH
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D019556:COS Cells / Q000502:physiology; D002352:Carrier Proteins / Q000502:physiology; D003902:Detergents; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000378:metabolism*; D000939:Epitopes / Q000032:analysis; D015870:Gene Expression / Q000502:physiology; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism*; D008322:Mammals; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D052066:N-Ethylmaleimide-Sensitive Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000302:isolation & purification; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D050765:Qa-SNARE Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D050683:R-SNARE Proteins; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D013347:Subcellular Fractions / Q000737:chemistry; D013572:Synaptic Vesicles / Q000737:chemistry / Q000378:metabolism*; D033921:Vesicular Transport Proteins
C098783:BET1 protein, rat; D002352:Carrier Proteins; D003902:Detergents; D000939:Epitopes; C490971:Gosr1 protein, mouse; C099509:Gosr1 protein, rat; C105309:Gosr2 protein, mouse; C491343:Gosr2 protein, rat; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D050683:R-SNARE Proteins; C098784:Sec22a protein, rat; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D033921:Vesicular Transport Proteins; D052066:N-Ethylmaleimide-Sensitive Proteins; C498733:Nsf protein, mouse; C498737:Nsf protein, rat