Multicellular tumor spheroids grown from pancreatic carcinoma cell lines: use as an orthotopic xenograft in athymic nude mice.
Carcinoma of the pancreas is the fifth leading cause of cancer death, with the majority of patients presenting with unresectable disease. Research into new adjuvant therapies is hampered by the lack of suitable in vivo and in vitro models. We have examined the potential of seven pancreatic carcinoma cell lines to grow as multicellular tumor spheroids (MTS). Three of seven (43%) cell lines were scored positive for MTS formation, and the growth characteristics for spheroid models of the cell lines PANC1 and MIAPaCa2 are presented. Implantation of PANC1 MTS into the pancreas of athymic nude mice produces tumors with reproducible growth characteristics and histology.
McLeod
E J
EJ
Department of Surgery, Melbourne Neuroscience Centre, University of Melbourne, Royal Melbourne Hospital, Australia.
Beischer
A D
AD
Hill
J S
JS
Kaye
A H
AH
D000818:Animals; D018929:Cell Culture Techniques; D002455:Cell Division; D006801:Humans; D007700:Kinetics; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008954:Models, Biological; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000473:pathology*; D018874:Spheroids, Cellular / Q000473:pathology* / Q000637:transplantation*; D014183:Transplantation, Heterologous