Insertional inactivation of dsbA produces sensitivity to cadmium and zinc in Escherichia coli.
In a search for genes that produce hypersensitivity to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used. One of the mutant strains obtained was sensitive to Cd2+ and Zn2+. Sequence analysis showed that the TnphoA insertion was located in the dsbA gene coding for a periplasmic protein required for disulfide bond formation.
Rensing
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C
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Mitra
B
B
Rosen
B P
BP
D000085:Acetates / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry; D002104:Cadmium / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D007535:Isomerases / Q000235:genetics*; D019216:Metals, Heavy / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D019704:Protein Disulfide-Isomerases; D017510:Protein Folding; D019287:Zinc Sulfate / Q000494:pharmacology*
D000085:Acetates; D001426:Bacterial Proteins; D019216:Metals, Heavy; D002104:Cadmium; D019287:Zinc Sulfate; C028031:cadmium acetate; D007535:Isomerases; D019704:Protein Disulfide-Isomerases