Effect of vitamin E deficiency and supercritical fluid aerosolized vitamin E supplementation on interleukin-1-induced oxidative lung injury in rats.
We hypothesized that alterations in lung vitamin E levels would impact the development of acute oxidative lung injury. We found that dietary induced deficiency of vitamin E diminished lung tissue levels of vitamin E and increased lung leak following intratracheal administration of interleukin-1 (IL-1) to rats. Conversely, rats administered vitamin E directly to the lungs as an inhaled aerosol (0.3-3 microns particles) formed by supercritical fluid aerosolization (SFA) had increased lung tissue vitamin E levels and decreased IL-1 induced lung leak compared to control rats. Lung myeloperoxidase (MPO) activities, reflecting neutrophil concentrations, were increased in rats given IL-1 intratracheally compared to rats given saline intratracheally but were not different for control or vitamin E depleted rats. Lung MPO activities in rats given IL-1 intratracheally were slightly higher in SFA vitamin E treated rats than in control rats. Our results suggest that vitamin E levels affect susceptibility to IL-1 induced, neutrophil-dependent lung injury. We speculate that supercritical fluid aerosol (SFA) delivery of vitamin E can rapidly increase lung vitamin E levels and decrease acute oxidative lung injury.
Hybertson
B M
BM
Waring Institute for Biomedical Research, University of Colorado Health Sciences Center, Denver, USA. HYBERTSO@ESSEX.HSC.COLORADO.EDU
Leff
J A
JA
Beehler
C J
CJ
Barry
P C
PC
Repine
J E
JE
D000328:Adult; D000336:Aerosols; D000818:Animals; D004032:Diet; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000633:toxicity; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D055370:Lung Injury; D008297:Male; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D014810:Vitamin E / Q000008:administration & dosage* / Q000493:pharmacokinetics; D014811:Vitamin E Deficiency / Q000150:complications* / Q000188:drug therapy / Q000378:metabolism
D000336:Aerosols; D005609:Free Radicals; D007375:Interleukin-1; D014810:Vitamin E; D009195:Peroxidase