Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD.
The genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotypes H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G + C contents. The amplified band arrays allowed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NM strains could be assigned to either H1 or H31 serotypes. The results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clonal markers in this E. coli group.
Pacheco
A B
AB
Laboratório de Fisiologia Celular, Universidade Federal do Rio de Janeiro, CCS, Cidade Universitária, Brazil. biafp@ibccf.biof.ufrj.br
Guth
B E
BE
Soares
K C
KC
de Almeida
D F
DF
Ferreira
L C
LC
D016172:DNA Fingerprinting; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics; D014644:Genetic Variation; D012703:Serotyping