Expression of mRNAs coding for the transforming growth factor-beta receptors in brain regions of euthyroid and hypothyroid neonatal rats and in adult brain.
The TGF-beta family of peptides has been postulated to play a role in control of the cell cycle but also may act in the developing brain to influence neuronal differentiation and survival. Because reception of TGF-beta signals requires the simultaneous expression of all three known receptor subtypes, we examined two neonatal rat brain regions in which neurogenesis has been largely completed. mRNA coding for all three receptors was detectable in both the forebrain and brainstem but only the type II receptor in brainstem showed a difference from adult levels of expression. Animals given perinatal PTU treatment to achieve congenital cretinism did not show significant differences in expression of any of the receptor subtypes in either of the regions, despite the fact that the treatment is known to cause anomalies of neuronal differentiation. These results indicate that regions in which neurons are undergoing axonogenesis and synaptogenesis rather than neurogenesis, nevertheless express the mRNAs coding for TGF-beta receptors and are thus likely to be receptive to trophic signals mediated through TGF-beta. However, synthesis and release of TGF-beta, rather than receptor expression per se, is more likely to be the major point for regulation of signaling. The potential roles of TGF-beta in developmental events outside of the cell cycle, such as synaptogenesis and apoptosis, need to be examined.
Slotkin
T A
TA
Department of Pharmacology, Duke University Medical Center, Durham, NC 27710, USA. slotk001@acpub.duke.edu
Wang
X F
XF
Symonds
H S
HS
Seidler
F J
FJ
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D003409:Congenital Hypothyroidism / Q000151:congenital / Q000378:metabolism*; D004195:Disease Models, Animal; D005815:Genetic Code; D007037:Hypothyroidism / Q000378:metabolism*; D016548:Prosencephalon / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D016212:Transforming Growth Factor beta / Q000235:genetics*
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta