Expression of murine interleukin 11 and its receptor alpha-chain in adult and embryonic tissues.
Interleukin 11 (IL-11) is a multifunctional cytokine that has diverse effects on blood cells and their precursors and on a number of cell types outside of the hematopoietic system. The cDNAs encoding murine IL-11 and its receptor alpha-chain (IL-11R alpha) have recently been isolated. We have used the RNase protection assay to examine the expression of murine IL-11 and IL-11R alpha in a range of adult mouse tissues, in embryos, and during development of embryonic stem (ES) cells into cystic embryoid bodies in vitro. The testis showed a high level of IL-11 gene expression while a much lower level of expression was detected in the lung, stomach, small intestine, and large intestine. Expression of IL-11 was not detected between day 10.5 and day 18.5 post coitum of embryonic development or in differentiating ES cells in vitro. In contrast, the IL-11R alpha was found to be expressed in all adult tissues examined, during embryonic development, and in totipotent and differentiating ES cells.
Davidson
A J
AJ
Department of Molecular Medicine, School of Medicine, University of Auckland, New Zealand.
Freeman
S A
SA
Crosier
K E
KE
Wood
C R
CR
Crosier
P S
PS
D000818:Animals; D002460:Cell Line; D004622:Embryo, Mammalian / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D015870:Gene Expression; D017370:Interleukin-11 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D053703:Interleukin-11 Receptor alpha Subunit; D051379:Mice; D008810:Mice, Inbred C57BL; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D053702:Receptors, Interleukin-11; D013234:Stem Cells / Q000378:metabolism
C508429:Il11ra1 protein, mouse; C508431:Il11ra2 protein, mouse; D017370:Interleukin-11; D053703:Interleukin-11 Receptor alpha Subunit; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11