High-resolution fluorescence in situ hybridization of human Y-linked genes on released chromatin.
Genes within the differential region of the human Y chromosome do not recombine, and therefore the determination of their location depends on physical mapping. Yeast artificial chromosome (YAC) contigs spanning the euchromatic region of the human Y have become a powerful tool for the generation of an overlapping clone map. With this approach, however, complete physical mapping is difficult in Y euchromatic regions that are rich in repetitive sequences. We have, therefore, made use of the fluorescence in situ hybridization technique as an alternative strategy for physically mapping the PRKY and AMELY genes as well as the TSPY, RBM and DAZ gene families to human Y chromosomes in prometaphase and to extended Y chromatin in interphase. From our results, the following order of gene sequences in interval 3 of the short arm of the human Y chromosome is suggested: TSPY major with few RBM sequences interspersed-PRKY-AMELY-TSPY minor with few RBM sequences interspersed-cen. On the long arm, RBM sequences appear to be distributed over wide regions of intervals 5 and 6 with few TSPY sequences interspersed. Distal to an RBM signal cluster, a large cluster of DAZ signals is located with only a few DAZ and RBM signals overlapping in between the two clusters.
Gläser
B
B
Institute of Human Genetics and Anthropology, University of Freiburg, Germany.
Hierl
T
T
Taylor
K
K
Schiebel
K
K
Zeitler
S
S
Papadopoullos
K
K
Rappold
G
G
Schempp
W
W
D018797:Cell Cycle Proteins; D002503:Centromere; D002843:Chromatin / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D018244:Chromosomes, Artificial, Yeast; D003360:Cosmids; D015342:DNA Probes; D004276:DNA, Satellite; D004268:DNA-Binding Proteins / Q000235:genetics; D000075364:Deleted in Azoospermia 1 Protein; D008040:Genetic Linkage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008677:Metaphase; D008856:Microscopy, Fluorescence; D005810:Multigene Family / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014998:Y Chromosome / Q000235:genetics*
D018797:Cell Cycle Proteins; D002843:Chromatin; C095668:DAZ1 protein, human; D015342:DNA Probes; D004276:DNA, Satellite; D004268:DNA-Binding Proteins; D000075364:Deleted in Azoospermia 1 Protein; D009687:Nuclear Proteins; C501499:RBMY1A1 protein, human; D016601:RNA-Binding Proteins; D051876:Sex-Determining Region Y Protein; C497749:TSPY1 protein, human; D014157:Transcription Factors