title: Novel Vibrio cholerae O139 genes involved in lipopolysaccharide biosynthesis.
abstract: The sequence of part of the rfb region of Vibrio cholerae serogroup O139 and the physical map of a 35-kb region of the O139 chromosome have been determined. The O139 rfb region presented contains a number of open reading frames which show similarities to other rfb and capsular biosynthesis genes found in members of the Enterobacteriaceae family and in V. cholerae O1. The cloned and sequenced region can complement the defects in O139 antigen biosynthesis in transposon insertions within the O139 rfb cluster. Linkage is demonstrated among IS1358 of V. cholerae O139, the rfb region, and the recently reported otnA and otnB genes (E. M. Bik, A. E. Bunschoten, R. D. Gouw, and F. R. Mooi, EMBO J. 14:209-216, 1995). In addition, the whole of this region has been linked to the rfaD gene. Furthermore, determination of the sequence flanking IS1358 has revealed homology to other rfb-like genes. The exact site of insertion with respect to rfaD is defined for the novel DNAs of both the Bengal and the Argentinian O139 isolates.
authors.affiliation: Department of Microbiology and Immunology, University of Adelaide, South Australia.
authors.forename: U H
authors.initials: UH
authors.lastname: Stroeher
authors.forename: G
authors.initials: G
authors.lastname: Parasivam
authors.forename: B K
authors.initials: BK
authors.lastname: Dredge
authors.forename: P A
authors.initials: PA
authors.lastname: Manning
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000096:biosynthesis; D001118:Argentina; D016667:Bacterial Capsules / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002238:Carbohydrate Epimerases / Q000235:genetics; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D007194:India; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014734:Vibrio cholerae / Q000235:genetics* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008070:Lipopolysaccharides; C079397:rfb protein, Bacteria; C037213:ADP-L-glycero-D-mannoheptose-6-epimerase; D002238:Carbohydrate Epimerases