Purification and characterization of pig inter-alpha-inhibitor and its constitutive heavy chains.
With the view of investigating the metabolism of inter-alpha-inhibitor, a plasma serine-proteinase inhibitor, in an animal model of inflammatory syndrome, we isolated inter-alpha-inhibitor from pig plasma. A high yield was obtained (140 mg/liter) with a two-step procedure: anion-exchange chromatography followed by affinity chromatography on heparin-Sepharose. In contrast to bovine inter-alpha-inhibitor was highly similar to human inter-alpha-inhibitor: its heavy chains are homologous to the human H1 and H2 heavy chains, as shown by chromatographic and electrophoretic properties, cross-immunoreactivity and N-terminal sequencing. Pig may therefore represent a good animal model to study inter-alpha-inhibitor metabolism and elucidate its physiological role.
Carrette
O
O
Laboratoire de Biochimie, Faculté de Pharmacie, Lille, France.
Mizon
C
C
Sautière
P
P
Sesboüé
R
R
Mizon
J
J
D000510:Alpha-Globulins / Q000737:chemistry* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D019811:Hydroxylamine; D006898:Hydroxylamines; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015842:Serine Proteinase Inhibitors / Q000737:chemistry*; D013552:Swine
D000510:Alpha-Globulins; D006898:Hydroxylamines; D046911:Macromolecular Substances; D015842:Serine Proteinase Inhibitors; D019811:Hydroxylamine; C011877:inter-alpha-inhibitor