title: Integrin receptor involvement in actin cable formation in an in vitro model of events associated with wound contraction.
abstract: Actin cables have been reported to act in vivo as contractile 'purse strings' capable of closing embryonic wounds through generation of circumferential tension. Furthermore, their involvement in wounds within in vitro model systems suggests that actin cable contraction may be an important mechanism involved in the process of wound closure. The aim of this study therefore, was to investigate the appearance of actin cables in a contracting fibroblast populated collagen lattice, an in vitro model of events associated with wound contraction. Utilising this in vitro model, the time-course of actin cable production was investigated and the involvement of integrin receptors analysed using immunofluorescent labelling techniques. Over a period of hours distinct cellular cable-like structures developed at the edges of collagen lattices coinciding with the onset of contraction. Cellular organisation within the cable was evident as was polymerisation of actin microfilaments into elongated stress fibres forming a continuous cell-cell 'actin cable' around the circumference of the lattice. Immunolocalisation demonstrated that integrin receptor subunits beta 1 and alpha 2 but not alpha 5 were involved in apparent intimate cell-cell contact between juxtaposed fibroblasts within this actin cable. This study demonstrates the involvement of integrin receptors in actin cable formation within collagen lattice systems undergoing reorganisation. Such integrin involvement may enable participating cells to respond to the tensional status of their surrounding environment and via cell-cell communication, to permit a co-ordinated contraction of the cable. It is concluded that integrin receptor involvement in active actin cable contraction may be involved in the process of wound contraction.
authors.affiliation: Department of Oral Surgery, Medicine and Pathology, Dental School, University of Wales College of Medicine, Cardiff, U.K.
authors.forename: P
authors.initials: P
authors.lastname: Stephens
authors.forename: P G
authors.initials: PG
authors.lastname: Genever
authors.forename: E J
authors.initials: EJ
authors.lastname: Wood
authors.forename: M J
authors.initials: MJ
authors.lastname: Raxworthy
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D003286:Contracture / Q000209:etiology / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D016023:Integrins / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000199:Actins; D016023:Integrins