Characterization of 6-deoxy-6-iodo-D-glucose: a potential new tool to assess glucose transport.
6-deoxy-6-iodo-D-glucose (6-DIG) was rapidly taken up by adipocytes. Insulin increased 6-DIG transport in adipocytes isolated from both rats and mice. This stimulation was more important in rat than in mouse adipocytes, in agreement with their respective amount of Glut 4 transporters. In two insulin resistant states, the biological behavior of 6-DIG and 3-O-methyl-D-glucose was similar. These results indicated that 6-DIG, which was transported into the cells via the glucose transporters, could be potentially useful to measure modifications of glucose transport.
Henry
C
C
Institut National de la Santé ET DE LA Recherche Medicale, Inserm U 145, Faculté Médicine, Nice, France.
Tanti
J F
JF
Grémeaux
T
T
Morin
C
C
Van Obberghen
E
E
Comet
M
M
Le Marchand-Brustel
Y
Y
D019325:3-O-Methylglucose / Q000493:pharmacokinetics; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000493:pharmacokinetics / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D051381:Rats; D017208:Rats, Wistar
C105926:6-deoxy-6-iodoglucose; D019325:3-O-Methylglucose; D003847:Deoxyglucose; D005947:Glucose