Effect of beraprost sodium on the increased transendothelial permeation of albumin induced by high concentration of glucose.
To determine whether a decrease in prostacyclin production is involved in the increase in endothelial permeability induced by a high concentration of glucose, we evaluated the effect of beraprost sodium, a stable prostacyclin analog, on the transendothelial permeation of albumin in cultured aortic cells. Permeation of albumin across an endothelial cell monolayer was significantly greater when the cells were cultured with a high concentration of glucose (400 or 800 mg/dl) than when they were cultured with 100 mg/dl glucose. No significant change in the permeation of albumin was observed when cells were cultured with 100 mg/dl glucose as compared with 100 mg/dl glucose plus 700 mg/dl mannitol. The addition of beraprost sodium to the culture medium completely restored the increase in the permeation of albumin brought about by 400 mg/dl glucose. These results suggest that increased transendothelial permeation of albumin by high glucose may be due in part to a decrease in prostacyclin production by the endothelial cells. Beraprost sodium may restore the endothelial barrier function affected by a high concentration of glucose.
Kobayashi
K
K
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka.
Umeda
F
F
Hashimoto
T
T
Mimura
K
K
Yamashita
T
T
Nawata
H
H
D000418:Albumins / Q000493:pharmacokinetics*; D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D003925:Diabetic Angiopathies / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005947:Glucose / Q000009:adverse effects / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*
D000418:Albumins; D014665:Vasodilator Agents; C048081:beraprost; D011464:Epoprostenol; D005947:Glucose