CMA3-banding pattern and fluorescence in situ hybridization with 18S rRNA genes in zebrafish chromosomes.
This study provides new data on zebrafish chromosomes, obtained from the chromomycin A3-banding pattern and mapping of 18S rRNA genes by fluorescence in situ hybridization (FISH). C-banding and Agstaining were also performed to analyse whether variation in heterochromatin and Ag-nucleolus organizer regions (NORs) exists among various commercially purchased strains. The results provide information on heterochromatin composition and on the existence of interindividual NOR polymorphism and contribute to the construction of an idiogram suitable for gene mapping.
Gornung
E
E
Department of Animal and Human Biology, University of Rome, Italy.
Gabrielli
I
I
Cataudella
S
S
Sola
L
L
D000818:Animals; D014128:Chromomycin A3; D002871:Chromosome Banding; D002874:Chromosome Mapping; D005260:Female; D005456:Fluorescent Dyes; D005819:Genetic Markers; D006570:Heterochromatin / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D009697:Nucleolus Organizer Region / Q000235:genetics; D011110:Polymorphism, Genetic; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*
D005456:Fluorescent Dyes; D005819:Genetic Markers; D006570:Heterochromatin; D012337:RNA, Ribosomal, 18S; D014128:Chromomycin A3