title: The influence of acute preoperative plasmapheresis on coagulation tests, fibrinolysis, blood loss and transfusion requirements in cardiac surgery.
abstract: OBJECTIVE
  Withdrawal of autologous plasma and reinfusion after cardiopulmonary bypass (CPB) offers the opportunity of improving patients' haemostasis and reducing homologous blood consumption in cardiac surgery. The influence of acute, preoperative plasmapheresis (APP) on coagulation tests, fibrinolysis, blood loss and transfusion requirements was investigated in elective aortocoronary bypass patients.
  
  
  METHODS
  Forty patients were randomized to a control or pheresis group. The pheresis group had platelet-rich plasmapheresis (PRP-group, n = 20) performed before incision and the platelet-rich plasma (PRP) was returned after CPB. The control group (n = 20) was managed without pheresis. All patients had serial coagulation studies, including prothrombin split products (F1/F2), fibrinopeptide A (FPA), protein C (PC), thrombomodulin (TM), tissue-plasminogen-activator (t-PA), plasminogen-activator-inhibitor (PAI 1), fibrinopeptide B beta 15-42 (FPB beta 15-42), haemoglobin and platelet counts determined intra- and postoperatively. Chest tube drainage and transfusion requirements were recorded.
  
  
  RESULTS
  APP had no negative effects on the quality of PRP. The platelet count of the withdrawn autologous plasma was 239 +/- 33 x 10(9)/l. From the end of the operation (after retransfusion of autologous plasma) until the first postoperative day platelet counts were significant higher in the PRP-group (P > 0.05). Plasma concentrations of modified antithrombin III (ATM), F1/F2 and FPA increased (166-290% from baseline) and PC- and TM-antigen decreased (11-49% from baseline) to a different extent for both groups throughout CPB. t-PA-activity increased intraoperatively peaking at the end of CPB (PRP-group: 4.8 +/- 0.8 IU/ml, control-group: 8.1 +/- 2.3 IU/ml)(P > 0.05). With onset of CPB PAI-1 levels decreased and were further reduced after CPB in control patients in comparison to PRP-patients (P < 0.05). FPB beta 15-42 occurred in peak concentrations after neutralisation of heparin by protamine. Only PRP-patients showed baseline values of coagulation and fibrinolytic parameters on the next morning (P < 0.05). Total postoperative blood loss during the first 24 h was 503 +/- 251 ml (PRP-group) and 937 +/- 349 ml in the control-group (P < 0.05). None of the PRP-patients received allogeneic blood, whereas five control-patients received 11 units of packed red cells (P < 0.05).
  
  
  CONCLUSIONS
  The findings suggest that in elective cardiac surgery heparin cannot prevent generation of both thrombin and fibrin, born throughout CPB and postoperatively. The use of PRP withdrawn immediately preoperatively is an attractive technique to reduce allogeneic blood usage and preoperative blood loss, especially in patients in whom withdrawal of autologous whole blood cannot be performed.
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, Giessen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Menges
authors.forename: I
authors.initials: I
authors.lastname: Welters
authors.forename: R M
authors.initials: RM
authors.lastname: Wagner
authors.forename: J
authors.initials: J
authors.lastname: Boldt
authors.forename: F
authors.initials: F
authors.lastname: Dapper
authors.forename: G
authors.initials: G
authors.lastname: Hempelmann
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001780:Blood Coagulation Tests; D016913:Blood Component Transfusion; D016063:Blood Loss, Surgical / Q000503:physiopathology*; D001804:Blood Transfusion, Autologous; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass; D005260:Female; D005342:Fibrinolysis / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010956:Plasmapheresis; D010976:Platelet Count; D010983:Plateletpheresis; D011183:Postoperative Complications / Q000097:blood*; D011300:Preoperative Care; D011446:Prospective Studies; D011486:Protein C / Q000378:metabolism; D018180:Thrombomodulin / Q000097:blood
chemical_list: D011486:Protein C; D018180:Thrombomodulin