title: Recombinant blood group antigens are useful for studying the fine specificity of antibodies against glycophorin A encoded antigens.
abstract: Recombinant wild-type and variant forms of glycophorin A (GPA) were expressed in wild-type and glycosylation-defective Chinese hamster ovary cells. The binding to recombinant GPA of antibodies submitted to the Third International Workshop was assessed by immunoprecipitation and indirect immunofluorescence. This is a powerful approach for determining the fine specificity of antibodies to blood group antigens. The advantages and limitations of this approach are discussed.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104, USA.
authors.forename: T
authors.initials: T
authors.lastname: Rozmyslowicz
authors.forename: M E
authors.initials: ME
authors.lastname: Donnell
authors.forename: S L
authors.initials: SL
authors.lastname: Spitalnik
authors.forename: D P
authors.initials: DP
authors.lastname: Blackall
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology*; D016466:CHO Cells; D006224:Cricetinae; D018604:Epitope Mapping; D005815:Genetic Code; D006021:Glycophorins / Q000235:genetics / Q000276:immunology*; D006031:Glycosylation; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D006021:Glycophorins; D007519:Isoantigens; D011994:Recombinant Proteins