Azelastine suppresses the release of arachidonic acid metabolites from cultured human umbilical vein endothelial cells.
We investigated the effects of azelastine, an anti-allergic agent, on the release of arachidonic acid metabolites from cultured human umbilical vein endothelial cells (HUVEC). High performance liquid chromatography (HPLC) revealed that HUVEC treated with 10(-5) M azelastine released smaller amounts of arachidonic acid metabolites into the medium than control HUVEC. Azelastine significantly reduced the release of prostaglandin F2 alpha (PGF2 alpha), leukotriene B4 (LTB4) and 5-hydroxy-eicosatetraenoic acid (5-HETE) by HUVEC. These results suggest that azelastine may inhibit contraction of bronchial smooth muscle cells and reduce bronchial inflammation by suppressing the release of arachidonic acid metabolites from vascular endothelial cells.
Watanabe
K
K
Second Department of Medicine, School of Medicine, Fukuoka University, Japan.
Nagasawa
N
N
Wen
F Q
FQ
Inoue
K
K
Kuroda
K
K
Yoshida
M
M
D018926:Anti-Allergic Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism*; D016084:Bronchoconstriction / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D015237:Dinoprost / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D007975:Leukotriene B4 / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D010793:Phthalazines / Q000494:pharmacology*; D014471:Umbilical Veins
D018926:Anti-Allergic Agents; D006893:Hydroxyeicosatetraenoic Acids; D010793:Phthalazines; D007975:Leukotriene B4; D016718:Arachidonic Acid; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D015237:Dinoprost; C020976:azelastine