Gas chromatographic-mass spectrometric determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol for study on human urinary excretion of ginsenosides after ingestion of ginseng preparations.
An improved gas chromatographic-mass spectrometric method (GC-MS) with a fast solid-phase extraction on a newly introduced C18 microcolumn, was applied to study the urinary excretion 20(S)-protopanaxadiol and 20(S)-protopanaxatriol glycosides in man after oral administration of ginseng preparations. Using panaxatriol as internal standard, 20(S)-protopanaxadiol and 20(S)-protopanaxatriol (the aglycones of ginsenosides) could be determined at a detection level of a few ng per ml urine by GC-MS with selected-ion monitoring after their release from glycosides which occur in urine. The extraction recovery of ginsenosides from urine was more than 80% and the intra-assay coefficient of variation was less than 5.0%. The results after intake of single doses of ginseng preparations demonstrated a linear relation between the amounts of ginsenosides consumed and the 20(S)-protopanaxatriol glycosides excreted in urine. About 1.2% of the dose was recovered in five days.
Cui
J F
JF
Department of Medical Laboratory Sciences and Technology, Karolinska Institute, Huddinge University Hospital, Sweden.
Björkhem
I
I
Eneroth
P
P
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D036145:Ginsenosides; D006801:Humans; D005894:Panax / Q000378:metabolism*; D010946:Plants, Medicinal; D012502:Sapogenins / Q000652:urine*; D012503:Saponins / Q000378:metabolism*; D014315:Triterpenes / Q000652:urine*
D036145:Ginsenosides; D012502:Sapogenins; D012503:Saponins; D014315:Triterpenes; C081552:protopanaxatriol; C062916:protopanaxadiol