Oxidation of low density lipoprotein in hemodialysis patients: effect of dialysis and comparison with matched controls.
End stage renal failure is associated with lipoprotein abnormalities and a high prevalence of premature atherosclerosis. Oxidative modification of low density lipoprotein (LDL) may be promoted by hemodialysis increasing its atherogenicity. The oxidative status of LDL was therefore examined in female subjects before and after routine hemodialysis (HD; n = 10) and compared with women of similar age without significant renal disease (n = 19). There were no significant differences between the groups in the LDL fatty acid composition, or in the content of reactive amino acid groups (lysine) before or after exposure to Cu2+. The kinetics of LDL oxidation by Cu2+ showed no significant differences between the groups with respect to the lag time, the level of conjugated dienes before and after oxidation, or the maximal rate of oxidation during the propagation phase. No acute effects of HD were demonstrated. The present study provides no evidence that circulating LDL isolated from HD patients is more extensively modified or more susceptible to oxidation in vitro than gender-matched controls without renal failure.
Westhuyzen
J
J
Department of Pathology, Royal Brisbane Hospital, Brisbane, Australia.
Saltissi
D
D
Healy
H
H
D000368:Aged; D001161:Arteriosclerosis / Q000097:blood / Q000209:etiology; D003300:Copper / Q000494:pharmacology; D005227:Fatty Acids / Q000097:blood; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000145:classification* / Q000150:complications / Q000628:therapy; D007700:Kinetics; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D008875:Middle Aged; D010084:Oxidation-Reduction; D006435:Renal Dialysis
D005227:Fatty Acids; D008055:Lipids; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D003300:Copper