B cell development under the condition of allelic inclusion.
Mice whose IgH alleles are engineered to encode two distinct antibody heavy (H) chains generate a normal-sized B cell compartment in which most cells stably express the two heavy chains. This demonstrates that "toxicity" of bi-allelic H chain expression and cell-autonomous mechanisms of silencing in-frame IgH gene rearrangements do not significantly contribute to allelic exclusion at the IgH locus. Notwithstanding, the stability of the various engineered IgH loci during B cell development in the bone marrow differed substantially from each other.
Sonoda
E
E
Institute for Genetics, University of Koln, Federal Republic of Germany.
Pewzner-Jung
Y
Y
Schwers
S
S
Taki
S
S
Jung
S
S
Eilat
D
D
Rajewsky
K
K
D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D002451:Cell Compartmentation / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D016368:Frameshift Mutation / Q000276:immunology; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism
D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region; D011947:Receptors, Antigen, B-Cell