B lymphocyte-specific, Cre-mediated mutagenesis in mice.
Adaptation of the P1 phage-derived Cre /loxP site- specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B lineage-restricted CD19 gene. In a model system involving the cross of CD19-Cre mice with mice bearing a loxP -flanked substrate, we find a deletion efficiency of 75-80% in bone marrow-derived pre-B cells that increases to 90-95% in splenic B cells.
Rickert
R C
RC
Institute for Genetics, University of Cologne, Weyertal 121, 50931 Cologne, Germany.
Roes
J
J
Rajewsky
K
K
D000818:Animals; D018941:Antigens, CD19 / Q000096:biosynthesis / Q000235:genetics*; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D015139:Blotting, Southern; D001853:Bone Marrow; D003433:Crosses, Genetic; D017931:DNA Primers; D017353:Gene Deletion; D005821:Genetic Techniques; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D007438:Introns; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016133:Polymerase Chain Reaction / Q000379:methods; D011995:Recombination, Genetic; D013154:Spleen / Q000166:cytology / Q000276:immunology
D018941:Antigens, CD19; D017931:DNA Primers