Structural studies of the O-specific chain of Hafnia alvei strain PCM 1190 lipopolysaccharide.
The structure of the O-specific side-chain of the lipopolysaccharide of Hafnia alvei strain PCM 1190 has been investigated. Methylation analysis, partial acid hydrolysis, Smith degradation, NMR spectroscopy, MALDI-TOF, and FAB mass spectrometry in combination with collision-induced-decomposition MS/MS were the principal methods used. It was concluded that the polysaccharide is composed of heptasaccharide repeating units having the following structure: [formula: see text] Only 80% of the repeating units are complete, whereas 20% of them are lacking the alpha-D-glucopyranosyl group.
Petersson
C
C
Department of Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Jachymek
W
W
Kenne
L
L
Niedziela
T
T
Lugowski
C
C
D002240:Carbohydrate Sequence; D004755:Enterobacteriaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D010084:Oxidation-Reduction; D016339:Spectrometry, Mass, Fast Atom Bombardment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D019081:O Antigens; D009844:Oligosaccharides