Stability of the antiproliferative effect of mitomycin-C after reconstitution.
OBJECTIVE
The purpose of this study was to determine the long-term stability of the antiproliferative effect of mitomycin-C (MMC) following reconstitution.


METHODS
Identical MMC preparations were reconstituted from the crystalline form and then stored at room temperature or 4 degrees C. Cultured human Tenon's fibroblasts were exposed to MMC (0.5 mg/ml) for 2.5 min at 0, 3, 7, 10, 14, 21, 28, 35, and 42 days following reconstitution. After removal of the drug, fibroblast proliferation was measured by tritiated thymidine uptake.


RESULTS
The percentage of inhibition was maintained at > 85% for both the room temperature and the 4 degrees C groups, and this inhibition persisted for the duration of the experiment. There was no statistically significant difference in the results between the storage temperatures.


CONCLUSIONS
These data suggest that MMC retains its antiproliferative effect for at least 6 weeks following reconstitution and that this effect is not changed by storage temperature. Cost savings may be realized by storing MMC in unit-dose reconstituted aliquots for these longer periods, providing sterility can be assured.
Woo
E
E
Ivey Institute of Ophthalmology, Victoria Hospital, University of Western Ontario, London, Canada.
Tingey
D P
DP
Mackenzie
G
G
Hooper
P
P
D000704:Analysis of Variance; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D004355:Drug Stability; D004356:Drug Storage; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007903:Lens Capsule, Crystalline / Q000187:drug effects / Q000473:pathology*; D016685:Mitomycin / Q000737:chemistry* / Q000494:pharmacology*; D019384:Nucleic Acid Synthesis Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D013696:Temperature
D003470:Culture Media; D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin