title: Producing vascularized bone by heterotopic bone induction and guided tissue regeneration: a silicone membrane-isolated latissimus dorsi island flap in a rat model.
abstract: Transformation of mesenchymal-type tissue into cartilage and bone can be induced by bone morphogenetic protein, and by its parent substratum, demineralized bone matrix. The authors were interested in transforming muscle island flaps into vascularized bone that could be used as autogeneic skeletal replacement parts. In Wistar rats, tubular latissimus dorsi muscle island flaps were created, using microsurgical techniques. The flaps were inserted by a cylinder of demineralized bone matrix (DBM) and enclosed in silicone rubber membrane tubes. The animals were followed-up for 10, 21, or 35 days. Rats with DBM implanted in muscle pouches served as controls. Quantitative radiomorphometry and qualitative histology were performed. A statistically significant linear time-related increase in radiomorphometrically-measured calcified tissue was found in the flaps with DBM from 10 days to 5 weeks. At 3 and 5 weeks, lamellar and cancellous bone with fully developed marrow was detected microscopically. There was no significant difference in bone quantity in the island flaps after 35 days, compared with the muscle pouches implanted with DBM, although the difference at 21 days was still significantly in favor of the island flaps. Using allogeneic DBM in rat muscle island flaps surrounded with a silicone membrane, it was possible to generate in vivo autogenous new bone with a good vascular supply and good mobility, allowing later transfer to another site. The experiment provided a basic technique that can be used as a standard in testing various osteoinductive substances for the production of vascular-pedicled new bone.
authors.affiliation: Bone Transplantation Research Group, University Central Hospital of Tampere, Finland.
authors.forename: V V
authors.initials: VV
authors.lastname: Viljanen
authors.forename: T J
authors.initials: TJ
authors.lastname: Gao
authors.forename: T S
authors.initials: TS
authors.lastname: Lindholm
mesh_terms: D000818:Animals; D001857:Bone Matrix; D019485:Bone Morphogenetic Proteins / Q000502:physiology*; D001842:Bone and Bones / Q000098:blood supply*; D008567:Membranes, Artificial; D008866:Microsurgery; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012828:Silicones; D013524:Surgical Flaps / Q000502:physiology*
chemical_list: D019485:Bone Morphogenetic Proteins; D008567:Membranes, Artificial; D012828:Silicones