Persistence of the dominant follicle during melengestrol acetate administration and its regression by exogenous estrogen treatment in beef cattle.
Experiments were conducted to test the hypothesis that estrogen treatment will regress a persistent dominant follicle developed during melengestrol acetate (MGA) treatment in the absence of a functional corpus luteum (CL) with normal fertility following development and ovulation of a newly recruited follicle. In Exp. 1, nonlactating beef cows (n = 31) were administered .5 mg.cow-1.d-1 of MGA (d 0) for 14 d with 25 mg of prostaglandin F2 alpha (PGF2 alpha) administered on d 6 and 8 to regress the CL. On d 11 of treatment, approximately half the MGA-treated cows received 5 mg of estradiol valerate (EV) i.m. (MGAEV, n = 14) and the remainder were maintained on MGA (n = 17). Ovaries were checked with ultrasound on d 8, 10, 12, and 14 of MGA treatment and every day until ovulation. A persistent dominant follicle developed in 90% of the MGA-treated cows by d 10 of treatment. Most of the MGA-treated cows ovulated the persistent dominant follicle (n = 13/17), whereas EV treatment regressed the persistent dominant follicle (n = 10/14) with the recruitment of a new follicle that ovulated (n = 8/10). Diameter of the ovulatory follicle was larger (P < .05) for the MGA (19.8 +/- .6 mm) than for the control (15.1 +/- .8 mm) and MGAEV (14.8 +/- .7 mm) cows. In Exp. 2, nonlactating, multiparous beef cows (n = 97) and yearling heifers (n = 38) were equally allotted to either a control, MGA alone, or MGA + estradiol-17 beta (MGAE) group with the same dose of MGA as administered in Exp. 1. The 1st d of MGA feeding was the 1st d of treatment. On d 10 of treatment half the MGA-treated animals were injected i.m. with 5 mg of estradiol-17 beta. In controls, behavioral estrus was detected and animals were artificially inseminated (AI) for 5 d (d 10 to 14 of experiment). All controls not exhibiting estrus by d 15 of experiment were injected with 25 mg of PGF2 alpha. The remaining controls and all MGA cows were observed for behavioral estrus and AI commenced for 7 d following withdrawal of MGA (d 15 to 21 of experiment). More (P < .05) controls (90.3%) than MGA (84.8%) or MGAE (63.6%) cows showed estrus within 7 d after MGA withdrawal. The percentage of animals conceiving to the synchronized estrus did not differ (P > .05) among treatments. The data support our hypothesis that a persistent dominant follicle developed and can be regressed with exogenous estrogen treatment followed by the recruitment and ovulation of a new follicle after MGA withdrawal and fertility of that estrus does not seem to be significantly compromised.
Yelich
J V
JV
Department of Animal Science, Oklahoma State University, Stillwater 74078-0425, USA.
Geisert
R D
RD
Schmitt
R A
RA
Morgan
G L
GL
McCann
J P
JP
D000818:Animals; D002417:Cattle / Q000097:blood / Q000502:physiology*; D015237:Dinoprost / Q000494:pharmacology; D004357:Drug Synergism; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D004971:Estrus / Q000187:drug effects / Q000502:physiology; D004973:Estrus Synchronization / Q000187:drug effects / Q000502:physiology; D005260:Female; D003341:Luteolysis / Q000187:drug effects* / Q000502:physiology; D008552:Melengestrol Acetate / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000000981:diagnostic imaging / Q000502:physiology; D010060:Ovulation / Q000187:drug effects / Q000502:physiology; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000097:blood; D045167:Progesterone Congeners / Q000494:pharmacology*; D011897:Random Allocation; D013997:Time Factors; D014463:Ultrasonography
D004967:Estrogens; D045167:Progesterone Congeners; D011374:Progesterone; D004958:Estradiol; D008552:Melengestrol Acetate; D015237:Dinoprost