Effects of pteridines on luminol-dependent chemiluminescence induced by chloramine-T.
Pteridines are ubiquitous in living organisms, but little is known about their biological functions. Different pteridines were tested for their ability to modulate luminol-dependent chemiluminescence induced by chloramine-T at pH = 7.5 and at a concentration of 100 microM for each pteridine. We observed striking differences between the compounds; whereas reduced pteridine species were generally potent scavengers, aromatic pteridines were weak to strong enhancers of the chemiluminescence. Taking into account the detailed chemical structure of the molecules, by multiple linear regression analysis a simple index was constructed that allows prediction of the effects of the different pteridines with high accuracy (linear correlation coefficient between predicted and observed values r = 0.89). The effects of different pteridines on free radical-induced chemiluminescence might bear biological significance since, for example, certain pteridines take part in enzymic reactions involving free-radical intermediates, or are related to the activation of macrophages in close relationship with the oxidative burst.
Reibnegger
G
G
Institute of Medical Chemistry and Biochemistry, University of Innsbruck, Austria.
Fuchs
D
D
Murr
C
C
Dierich
M P
MP
Pfleiderer
W
W
Wachter
H
H
D000818:Animals; D002700:Chloramines / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D008262:Macrophage Activation; D015394:Molecular Structure; D011621:Pteridines / Q000737:chemistry*; D016897:Respiratory Burst; D014105:Tosyl Compounds / Q000737:chemistry*
D002700:Chloramines; D016166:Free Radical Scavengers; D005609:Free Radicals; D011621:Pteridines; D014105:Tosyl Compounds; C016300:chloramine-T; D008165:Luminol