Activation of LA-N-2 cell phospholipases by single alanine substitution analogs of amyloid beta peptide (25-35).
A series of single alanine substituted analogs of amyloid beta peptide (25-35) were tested for their ability to activate the phospholipases of cultured LA-N-2 cells. Substitution of alanine for the amino acids 29-34 prevented the activation of phospholipases A2 and D. In addition substitution of alanine at 28 prevented phospholipase D but not phospholipase A2 activation. All the alanine substitutions, except for positions 33 and 35, blunted phospholipase C activations. There were no activations by scrambled amyloid beta peptide.
Singh
I N
IN
Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.
Sato
K
K
Takashima
A
A
Kanfer
J N
JN
D000409:Alanine / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000494:pharmacology*; D004789:Enzyme Activation; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D010739:Phospholipase D / Q000187:drug effects* / Q000378:metabolism; D010741:Phospholipases A / Q000187:drug effects* / Q000378:metabolism; D054467:Phospholipases A2; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000187:drug effects* / Q000378:metabolism
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases; D010739:Phospholipase D; D000409:Alanine