Involvement of PA/plasmin system in the processing of pro-MMP-9 and in the second step of pro-MMP-2 activation.
Pro-MMP2 activation is a two-step process resulting in (1) an intermediate 64 kDa form generated by the MT1-MMP activity, and (2) a mature 62 kDa form. Addition of plasminogen to HT1080 cells cultured under various conditions, or to their membrane preparation, induced a complete conversion of the intermediate MMP-2 form to the mature one, and processing of pro-MMP-9. The pro-MMP-2 activation was inhibited by plasmin inhibitors and anti-uPA antibody. These results provide evidence for involvement of the PA/plasmin system in the second step of MMP-2 activation.
Baramova
E N
EN
Laboratory of Biology, University of Liege, Belgium.
Bajou
K
K
Remacle
A
A
L'Hoir
C
C
Krell
H W
HW
Weidle
U H
UH
Noel
A
A
Foidart
J M
JM
D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism*; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism*; D018093:Gelatinases / Q000378:metabolism*; D006801:Humans; D008566:Membranes / Q000378:metabolism; D008666:Metalloendopeptidases / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D014407:Tumor Cells, Cultured
D004792:Enzyme Precursors; D010960:Plasminogen Activators; D005341:Fibrinolysin; D018093:Gelatinases; D008666:Metalloendopeptidases; C069860:progelatinase; D003012:Microbial Collagenase