A rapid tissue culture assay for the detection of okadaic acid and related compounds in mussels.
Okadaic acid, the main toxin of the diarrhoetic shellfish poisoning (DSP) group, shows high cytotoxicity to Buffalo green monkey (BGM) kidney cell cultures after different times according to concentration. A rapid method of detecting the presence of okadaic acid and related compounds in contaminated mussels by means of a cytotoxicity test is presented. The method is based on direct microscopic observation of toxin-induced morphological changes in BGM cell cultures. A high correlation (r = 0.950; P < 0.001) was found between the official mouse bioassay and the cytotoxicity test conducted on naturally contaminated samples of Mytilus galloprovincialis.
Croci
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Reparto Igiene delle Technologie Alimentari Laboratorio Alimenti, Istituto Superiore di Sanità, Roma, Italy.
Cozzi
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Stacchini
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De Medici
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Toti
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D000818:Animals; D049872:Bivalvia; D002522:Chlorocebus aethiops; D046508:Culture Techniques; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity; D051379:Mice; D019319:Okadaic Acid / Q000032:analysis* / Q000633:toxicity
D008387:Marine Toxins; D019319:Okadaic Acid