Genetic determination of polygalacturonase production in wild-type and laboratory strains of Saccharomyces cerevisiae.
The genetic determination of polygalacturonase (PG) production in Saccharomyces cerevisiae was studied by biochemical and classical genetic techniques. Crosses of PG+ strains with PG- strains showed that in the haploid wild-type-derived strain, two structural genes were involved in the production of a hydrolysis halo on plates with polygalacturonic acid. However, in the case of PG+ laboratory strain IM1-8b, the phenotype was controlled by only one structural gene although the analysis of PG- IM1-8b mutants demonstrated the existence of at least two complementation groups. All these genetic results were assessed biochemically by means of cation-exchange chromatography. Two enzymes were separated in the wild-type strain, and only one in the laboratory strain. The three enzymes had different Km values, molecular masses, and optimal pHs for activity.
Blanco
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P
Department of Microbiology, Faculty of Pharmacy, University of Santiago, Santiago de Compostela 15706, Spain.
Sieiro
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C
Reboredo
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NM
Villa
T G
TG
D003433:Crosses, Genetic; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009154:Mutation; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*
D011096:Polygalacturonase