title: A single point mutation of hamster aminoacyl-tRNA synthetase causes apoptosis by deprivation of cognate amino acid residue.
abstract: BACKGROUND
  We have isolated a series of temperature-sensitive mutants for cell-proliferation from the BHK21 cell line derived from the golden hamster (Nishimoto & Basilico 1978; Nishimoto et al. 1982). Using these mutants as a recipient of DNA-mediated gene transfer, we have been cloning human genes which complement these ts mutants.
  
  
  RESULTS
  Cultures of tsBN269 cells, a temperature-sensitive mutant of the BHK21 cell line, underwent apoptosis at 39.5 degrees C, a nonpermissive temperature. The gene complementing the tsBN269 cells was cloned and found to encode lysyl-tRNA synthetase. Indeed, tsBN269 cells were found to have a single cytosine to a thymine point mutation at the first nucleotide of codon 542 in hamster lysyl-tRNA synthetases. Due to this mutation, the activity of lysyl-tRNA synthetase was reduced--even at 33.5 degrees C, a permissive temperature. Consistent with these findings, while supplementation with lysine permitted tsBN269 cells to grow at a nonpermissive temperature, the deprivation of lysine caused apoptosis in tsBN269 cells, even at 33.5 degrees C. Cycloheximide inhibited the apoptosis caused by lysine starvation at 33.5 degrees C, but not at 39.5 degrees C. We also found that another hamster temperature-sensitive mutant, tsBN250, which is defective in histidyl-tRNA synthetase, entered apoptosis with the deprivation of histidine.
  
  
  CONCLUSIONS
  Our data suggested that the defect in aminoacyl-tRNA synthetase turned on the cascade of apoptosis that was already present in the cells.
authors.affiliation: Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Higashi-ku, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fukushima
authors.forename: S
authors.initials: S
authors.lastname: Motomura
authors.forename: A
authors.initials: A
authors.lastname: Kuraoka
authors.forename: H
authors.initials: H
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Nishimoto
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D006224:Cricetinae; D053938:DNA Fragmentation; D007668:Kidney; D008239:Lysine; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D013696:Temperature
chemical_list: D000596:Amino Acids; D008250:Lysine-tRNA Ligase; D008239:Lysine