title: Effects of impaired maternal insulin secretion on preimplantation embryo development in ICR mice.
abstract: To investigate the significance of impaired insulin secretion on preimplantation embryo development, outbred ICR female mice received an injection of a single dose of streptozotocin 200 mg.kg-1 14-17 days before fertilization. Oocytes were collected 24-26 h after hCG injection. Morphological evaluation revealed a lower percentage of oocytes with second polar bodies from streptozotocin-treated females in comparison with controls. Furthermore, in this group the incidence of degenerated embryos significantly increased after 120 h in vitro cultivation. Insulin (5 U per 100 g b.w.) administered twice daily to streptozotocin-treated mice significantly improved the Embryonic development. Morphological analysis of oocyte maturation in streptozotocin-treated mice showed no significant differences in comparison with control mice. It could be concluded that marked changes in preimplantation embryo development were detected in outbred ICR mice after streptozotocin administration and this process was partly reversible by insulin treatment. Furthermore, it was shown that the process of fertilization was negatively influenced and that during in vitro cultivation the delayed effects of impaired insulin secretion resulted in an increase of embryo degeneration at the time following the third mitotic cleavage.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovak Republic.
authors.forename: P
authors.initials: P
authors.lastname: Rehák
authors.forename: J
authors.initials: J
authors.lastname: Mihálik
authors.forename: J
authors.initials: J
authors.lastname: Veselá
authors.forename: S
authors.initials: S
authors.lastname: Cikos
authors.forename: V
authors.initials: V
authors.lastname: Baran
authors.forename: J
authors.initials: J
authors.lastname: Koppel
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D001786:Blood Glucose / Q000378:metabolism; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005260:Female; D005306:Fertilization / Q000502:physiology; D007328:Insulin / Q000097:blood; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects / Q000648:ultrastructure; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000503:physiopathology*
chemical_list: D001786:Blood Glucose; D006063:Chorionic Gonadotropin; D007328:Insulin