Structure of the repeating oligosaccharide from the lipopolysaccharide of the nitrogen-fixing bacterium Acetobacter diazotrophicus strain PAL 5.
Acetobacter diazotrophicus is an acid-tolerant nitrogen-fixing bacterium found in roots, rhizosphere, stems, and leaves of sugar cane (Saccharum officinarum) cultivated in Brazil. The O-polysaccharide from the lipopolysaccharide of the root isolate strain PAL 5 has been determined by a combination of methylation analysis and two-dimensional high field NMR spectroscopy. The pentasaccharide repeat has the structure: [formula: see text] Minor resonances in the NMR spectra are consistent with the presence of a proportion of repeating units which lack the beta-D-Glc side-chain.
Previato
J O
JO
Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Brasil.
Jones
C
C
Stephan
M P
MP
Almeida
L P
LP
Mendonça-Previato
L
L
D000091:Acetobacter / Q000737:chemistry*; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000502:physiology*; D009844:Oligosaccharides / Q000737:chemistry*
D008070:Lipopolysaccharides; D009844:Oligosaccharides