title: Molecular epidemiology of human rotaviruses in Santiago, Chile.
abstract: BACKGROUND
  Protective immunity against rotavirus infection is directed against antigenic epitopes on the outer capsid proteins VP7 and VP4. Our aim was to characterize the epidemiology of rotavirus antigenic types over time in Santiago, Chile.
  
  
  METHODS
  We prospectively obtained 2097 stool samples for rotavirus testing, VP7 (G1 to G4) and VP4 (P4, P6, P8, P9) typing from children with diarrhea evaluated in emergency rooms of 5 base hospitals of Santiago. In addition 256 rotavirus-positive samples collected between 1985 and 1987 in the north health care area of Santiago were studied.
  
  
  RESULTS
  Of 995 rotavirus-positive samples obtained 825 (82%) were typable for 1 or more VP7 types. G1 represented 81% of the G-typed samples during 1993 through 1995 and 77% during 1985 through 1987, predominating in all health care areas. G2 was next most common in all 5 areas, representing 6 to 23% of typed samples, with 1 area, the Southeast concentrating a significantly higher number of G2 infections. G2 declined from 35% of rotavirus-positive samples in 1993 to 0% in 1995 (P < 0.001), and from 25% to 2% in the north health care area from 1985 to 1987 (P < 0.001). G4 was uncommon and significantly more prevalent in 1985 through 1987 than in 1993 through 1995 (7% vs. 3%, P = 0.015). G3 was not detected. G1P8 (53%) and G2P4 (16%) combinations were by far the most commonly detected G-P associations.
  
  
  CONCLUSIONS
  In Santiago, Chile, rotavirus antigenic type G1P8 has been highly prevalent and G2P4 has circulated in cycles. Differences in epidemiology of rotavirus antigenic types worldwide may prove to be relevant in efficacy of rotavirus vaccines.
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Chile, Santiago, Chile. moryan@machi.med.uchile.cl
authors.forename: M L
authors.initials: ML
authors.lastname: O'Ryan
authors.forename: N
authors.initials: N
authors.lastname: Mamani
authors.forename: L F
authors.initials: LF
authors.lastname: Avendaño
authors.forename: J
authors.initials: J
authors.lastname: Cohen
authors.forename: A
authors.initials: A
authors.lastname: Peña
authors.forename: J
authors.initials: J
authors.lastname: Villarroel
authors.forename: A
authors.initials: A
authors.lastname: Chavez
authors.forename: F
authors.initials: F
authors.lastname: Valdivieso
authors.forename: D O
authors.initials: DO
authors.lastname: Matson
mesh_terms: D002648:Child; D002677:Chile / Q000453:epidemiology; D003967:Diarrhea / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology*