title: MIB-1 (Ki-67) immunostaining of breast cancer cells in cytologic smears.
abstract: OBJECTIVE
  The reliability of immunocytochemical evaluation of proliferation activity was tested using the monoclonal antibody MIB-1 on cytologic specimens.
  
  
  METHODS
  The study comprised 83 frozen tissue smears (FTSs) and 51 fine needle aspirates (FNAs) from 119 breast cancer patients. MIB-1 labeling indexes (LIs) were compared with various tumor parameters assessed on histologic material.
  
  
  RESULTS
  MIB-1 LIs established on cytologic smears were significantly different in ductal and lobular carcinomas (P = .024) and correlated significantly with mitotic activity (P < .0001), histologic grade (P < .0001) and S-phase fraction (P < .0001). Essentially the same results were obtained on FTSs and FNAs.
  
  
  CONCLUSIONS
  Proliferative activity can reliably be evaluated by FNA cytology, and the evaluation of MIB-1 LIs may complement cytologic grading of breast cancer. The evaluation of proliferation activity may, therefore, contribute to the selection of candidates for adjuvant chemotherapy.
authors.affiliation: Institute for Pathology, University of Basel, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Dalquen
authors.forename: B
authors.initials: B
authors.lastname: Baschiera
authors.forename: R
authors.initials: R
authors.lastname: Chaffard
authors.forename: H
authors.initials: H
authors.lastname: Dieterich
authors.forename: G E
authors.initials: GE
authors.lastname: Feichter
authors.forename: K
authors.initials: K
authors.lastname: Krmer
authors.forename: J
authors.initials: J
authors.lastname: Torhorst
mesh_terms: D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology*; D018275:Carcinoma, Lobular / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000592:standards*; D019394:Ki-67 Antigen / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D019394:Ki-67 Antigen