title: Granulocyte macrophage colony stimulating factor pretreatment induces an increase of rat Kupffer cells with enhanced cytotoxicity in vitro and prevention of tumor outgrowth in vivo.
abstract: Male Wag rats were pretreated for 7 days with 1000 U/ml recombinant murine granulocyte macrophage colony stimulating factor (rmGM-CSF). Rat Kupffer cells (KC) were isolated by a enzymatic method. We injected decreasing numbers of CC531 tumor cells in the portal system. Mean KC yield increased from 1.5 +/- 0.2 to 2.2 +/- 0.2 (p < 0.05). Mean percentage of KC-mediated cytotoxicity against CC531 increased from 20.0 +/- 0.5 to 42 +/- 1.0 after rmGM-CSF (p < 0.05). At 1 x 10(5) CC531 tumor cells we demonstrated prevention of formation of small foci of CC52+ tumor cells. We demonstrate increased isolated KC with enhanced cytotoxic capacity after rmGM-CSF. rmGM-CSF induced prevention of minimal residual disease in the rat liver.
authors.affiliation: Department of Surgical Oncology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Schuurman
authors.forename: H J
authors.initials: HJ
authors.lastname: Koenen
authors.forename: R H
authors.initials: RH
authors.lastname: Beelen
authors.forename: S
authors.initials: S
authors.lastname: Meyer
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000188:drug therapy; D016923:Cell Death / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology* / Q000627:therapeutic use; D007150:Immunohistochemistry; D007728:Kupffer Cells / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control / Q000556:secondary; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D016179:Granulocyte Colony-Stimulating Factor; C022616:thiazolyl blue; D013936:Thymidine