Mitogenic activities of water-soluble and -insoluble insulin conjugates.
Insulin was covalently bound to water-soluble polymers such as poly(oxyethylene) and poly(acrylic acid). The former and the latter product are water-soluble monovalent and multivalent conjugates, respectively. Insulin was also bound to a poly(acrylic acid)-grafted polystyrene film, to form a water-insoluble multivalent conjugate. The matrix polymer was prepared by graft polymerization of acrylic acid initiated by glow-discharged polystyrene film. Insulin coupled with poly(oxyethylene) reduced the mitogenic activity, but the poly(acrylic acid)-insulin conjugate stimulated cell growth more than native insulin. A concentration of immobilized insulin much lower than that of native insulin and the water-soluble insulin conjugates accelerated cell growth. The maximal mitogenic effect of the immobilized insulin was greater than that of native insulin or the water-soluble insulin conjugates. The findings suggest that the mitogenic effect of the water-insoluble, multivalent insulin conjugate lasts longer than that of the water-soluble conjugates, owing to the absence of internalization into the cell.
Chen
G
G
Department of Material Chemistry, Faculty of Engineering, Kyoto University, Japan.
Ito
Y
Y
Imanishi
Y
Y
D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D007328:Insulin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology*; D051379:Mice; D008934:Mitogens / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D012995:Solubility; D014867:Water
D000180:Acrylic Resins; D007328:Insulin; D008934:Mitogens; D014867:Water; D011092:Polyethylene Glycols; C006903:carbopol 940; D004247:DNA