Stem cell antigen 2 expression in adult and developing mice.
Stem cell antigen 2 (Sca-2) expression can distinguish the most immature T-lymphocyte precursors in the thymus from the hemopoietic stem cells. Sequence analysis of the Sca-2 protein showed that Sca-2 is a glycosylphosphatidylinositol (GPI) anchored molecule that shares some characteristics with the members of the Ly-6 multigene family, and that it is the same as the thymic shared antigen-1 (TSA-1). Here we extend these studies and critically reassess the expression of the Sca-2/TSA-1 antigen in hematopoietic tissues of adult and developing mice. With more sensitive methods we show that the distribution of Sca-2/TSA-1 differs from existing reports. We find especially high expression of Sca-2/TSA1 at day 14 of fetal development.
Antica
M
M
Department of Molecular Biology and Medicine, Ruder Bosković Institute, Zagreb, Croatia. Antica@olimp.irb.hr
Wu
L
L
Scollay
R
R
D000818:Animals; D001853:Bone Marrow / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D006410:Hematopoiesis; D006413:Hematopoietic System / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000378:metabolism; D008221:Lymphoid Tissue / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D013154:Spleen / Q000196:embryology / Q000254:growth & development / Q000378:metabolism
D008565:Membrane Proteins; C084839:thymic shared antigen-1