title: Intestinal lipid esterification and aging in mice and rats.
abstract: The effects of aging on lipid absorption, particularly on fatty acid glycerophospholipid and triacyl-glycerol esterification, were investigated in 2.5-, 12- and 24-month-old mice and rats. Two intestinal mucosa microsomal enzymes, involved in the dietary fatty acid absorption, were assayed: acylCoA:2-monoacylglycerol acyltransferase and acylCoA:1-lysophosphatidylcholine acyltransferase. In both mice and rats, the activities of both enzymes varied with the nature of the acyl-CoA. Indeed acylCoa:2-monoacylglycerol acyltransferase activities were significantly higher with oleoyl-CoA and linoleoyl-CoA than with palmitoyl-CoA and arachidonoyl-CoA, while acylCoA:1-lysophosphatidylcholine acyltransferase activities were highest with arachidonoyl-CoA. AcylCoA:2-monoacylglycerol acyltransferase activity did not decrease significantly with aging in mice or rats, whatever the acyl-CoA used. In contrast, acylCoA:1-lysophosphatidylcholine acyltransferase activity in the 24-month-old rats was significantly lower (-47%) than in 2.5-month-old rats, with oleoyl-CoA, linoleoyl-CoA and arachidonoyl-CoA. Simultaneously we observed that less glycerophospholipid esterification of oleic and linoleic acid occurs in older rats than in 2.5-month-old rats.
authors.affiliation: Laboratoire de Physiologie de la Nutrition, EA DRED 580, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Université de Bourgogne, Dijon, France.
authors.forename: H
authors.initials: H
authors.lastname: Poirier
authors.forename: Y
authors.initials: Y
authors.lastname: Mathieu
authors.forename: P
authors.initials: P
authors.lastname: Besnard
authors.forename: A
authors.initials: A
authors.lastname: Bernard
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D004951:Esterification; D007413:Intestinal Mucosa / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar