Comparison of sorbitol MacConkey and hemorrhagic coli agars for recovery of Escherichia coli O157:H7 from brie, ice cream, and whole milk.
The relative efficacies of hemorrhagic coli (HC) agar and several formulations of sorbitol MacConkey (SorMac) agar, with and without 0.1% (w/v) 4-methyllumbelliferyl-beta-D-glucuronide (MUG), in recovering unstressed and heat-stressed Escherichia coli O157:H7 from Brie cheese, ice cream, and whole milk were determined. Recovery of unstressed E. coli O157:H7 was determined quantitatively by spread-plating diluted samples onto different agars and performing plate counts. Recovery of stressed E. coli O157:H7 was determined qualitatively by enriching samples in modified trypticase soy broth, streaking the incubated enrichments, and isolating E. coli O157:H7 colonies from the agars. HC agar and the SorMac agar formulations did not differ significantly in their ability to recover unstressed E. coli O157:H7 from ice cream and whole milk; however, HC agar recovered significantly more unstressed E. coli O157:H7 from Brie cheese than did the SorMac agar formulations. Bacteriological Analytical Manual and Oxoid SorMac agar formulations made from individual ingredients, did not differ significantly in recovering unstressed E. coli O157:H7 from Brie cheese. The efficiency of the commercially available Oxoid SorMac agar could not be determined because of overgrowth by indigenous microflora. HC and SorMac agars did not differ significantly in recovering stressed E. coli O157:H7 from Brie cheese, ice cream, and whole milk. MUG had no apparent effect on recovery of either stressed or unstressed E. coli O157:H7 from the dairy foods examined.
Hammack
T S
TS
U.S. Food and Drug Administration, Division of Microbiological Studies, Washington, DC 20204, USA.
Feng
P
P
Amaguaña
R M
RM
June
G A
GA
Sherrod
P S
PS
Andrews
W H
WH
D000362:Agar; D000818:Animals; D002611:Cheese; D003470:Culture Media; D015342:DNA Probes / Q000032:analysis; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005516:Food Microbiology; D007054:Ice Cream / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D013012:Sorbitol
D003470:Culture Media; D015342:DNA Probes; D013012:Sorbitol; D000362:Agar