Organization and transcription of the gene encoding potato UDP-glucose pyrophosphorylase.
The organization of the gene encoding potato UDP-glucose pyrophosphorylase, one of the key enzymes of carbohydrate metabolic pathway is presented. The gene cloned from cultivar (cv.) Lemhi consists of a 6.6-kb structural and a 1-kb regulatory region. The structural region contains 20 exons and 19 introns. The coding sequence with exception of three bases is identical with the UGPase cDNA previously cloned from Danshaku-Imo cv. [Katsube et al. (1990) UDP-Glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing. J. Biochem. 108, 321-326]. The largest intron contains a tandem repeat consisting of 50 nt core units. A putative polyadenylation site is situated 79 bp downstream of the translation stop codon. A transcription start point (tsp) and a putative TATA-box were located 84 bp and 141 bp upstream of the translation start, respectively. The regulatory region contained general enhancer, suppressor, and regions responsible for tissue specificity of UGPase expression.
Borovkov
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AY
Department of Plant Pathology, North Dakota State University, Fargo 58105, USA. borovkov@plains.nodak.edu
McClean
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PE
Secor
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GA
D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D007438:Introns; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics*; D014158:Transcription, Genetic; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000096:biosynthesis* / Q000235:genetics*
D011994:Recombinant Proteins; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase