A fluoroimmunoassay based on immunoliposomes containing genetically engineered lipid-tagged antibody.
Immunoliposomes were prepared by using biosynthetically lipid-tagged anti-2-phenyloxazolone single-chain antibody. Carboxyfluorescein as a fluorescent marker was encapsulated in the immunoliposomes. Some conditions for fluoroimmunoassay using the immunoliposomes were optimized by binding assays with hapten-coated microtiter wells. A competitive fluoroimmunoassay for the caproic acid conjugate of 2-phenyloxazolone as a model antigen was performed with the immunoliposomes. In the optimized assay conditions, antigen could be determined in the concentration range from 10(-7) to 10(-9) M.
Kobatake
E
E
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Sasakura
H
H
Haruyama
T
T
Laukkanen
M L
ML
Keinänen
K
K
Aizawa
M
M
D001667:Binding, Competitive; D005452:Fluoresceins; D005456:Fluorescent Dyes; D015200:Fluoroimmunoassay; D005818:Genetic Engineering; D006241:Haptens; D008081:Liposomes / Q000276:immunology*; D010081:Oxazolone / Q000031:analogs & derivatives
D005452:Fluoresceins; D005456:Fluorescent Dyes; D006241:Haptens; D008081:Liposomes; C018361:2-phenyloxazolone; D010081:Oxazolone; C024098:6-carboxyfluorescein