Clonostachin, a novel peptaibol that inhibits platelet aggregation.
A novel peptaibol, designated clonostachin, was isolated from cultures of Clonostachys sp. F5898 by HP-20 and silica gel column chromatographies and reverse-phase HPLC. The structure of clonostachin was determined by Edman and chemical degradations, positive ion FAB-MS, EI-MS, and NMR analyses. Clonostachin was a linear tetradecapeptide with an N-terminal acetyl group and a C-terminal sugar alcohol. Clonostachin inhibited ADP-induced aggregation of human platelets by 80% at 150 microM.
Chikanishi
T
T
Department of Applied Biological Science, Tokyo Noko University, Japan.
Hasumi
K
K
Harada
T
T
Kawasaki
N
N
Endo
A
A
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008795:Metronidazole / Q000737:chemistry / Q000494:pharmacology*; D003904:Mitosporic Fungi / Q000737:chemistry / Q000145:classification; D015394:Molecular Structure; D010455:Peptides; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000900:Anti-Bacterial Agents; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; D008795:Metronidazole; D000244:Adenosine Diphosphate