A detailed lectin analysis of IgG glycosylation, demonstrating disease specific changes in terminal galactose and N-acetylglucosamine.
Serum IgG from rheumatoid arthritis patients contains a decreased number of oligosaccharide structures ending in galactose and thus there is an increase in N-acetylglucosamine as the terminal sugar, compared with healthy individuals. The relationship between these two sugars varies depending on the disease examined: IgG from patients with rheumatoid arthritis, juvenile onset chronic arthritis and Crohn's disease are at one extreme, and exhibit a reciprocal galactose:N-acetylglucosamine relationship, while Sjögren's syndrome and osteoarthritis IgG are at the other extreme, exhibiting a parallel increase in the expression of both galactose and N-acetylglucosamine. These results may occur as a consequence of more than one glycosylation site which is differentially glycosylated, but more likely by changes in the level of bisecting N-acetylglucosamine.
Bond
A
A
Division of Immunology, St George's Hospital Medical School, London. a.bond@sghms.ac.uk
Alavi
A
A
Axford
J S
JS
Bourke
B E
BE
Bruckner
F E
FE
Kerr
M A
MA
Maxwell
J D
JD
Tweed
K J
KJ
Weldon
M J
MJ
Youinou
P
P
Hay
F C
FC
D000117:Acetylglucosamine / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism; D008875:Middle Aged; D009844:Oligosaccharides / Q000378:metabolism; D012859:Sjogren's Syndrome / Q000276:immunology / Q000378:metabolism
D007074:Immunoglobulin G; D037102:Lectins; D009844:Oligosaccharides; D000117:Acetylglucosamine; D005690:Galactose