Measurement and pharmacokinetic analysis of buspirone by means of brain microdialysis coupled to high-performance liquid chromatography with electrochemical detection.
The feasibility of an electrochemical detection system with on-line microdialysis coupled with sensitive microbore high-performance liquid chromatography for the measurement and brain pharmacokinetic analysis of buspirone was investigated. A microdialysis probe was inserted into the right striatum of male Sprague-Dawley rats, which had been administered buspirone 10 mg/kg. i.v.). Dialysates were automatically injected through an on-line injector into a cyano microbore column coupled to an electrochemical detector. Samples were eluted with a mobile phase containing 0.1 M monosodium dihydrogenphosphate acetonitrile-diethylamine (85:15:0.1, v/v/v). pH 3.0, adjusted with orthophosphoric acids at a flow-rate of 0.06 ml/min. A biphasic phenomenon with a rapid distribution phase followed by a slower elimination phase was observed from the brain buspirone concentration-time curve. The results indicate that the brain pharmacokinetics of buspirone appear to conform to a two-compartment model.
Tsai
T H
TH
Department of Pharmacology, National Research Institute of Chinese Medicine, Taipei, Taiwan.
Chen
C F
CF
D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D001921:Brain / Q000378:metabolism*; D002065:Buspirone / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D004563:Electrochemistry; D008297:Male; D017551:Microdialysis; D009862:Online Systems; D009994:Osmolar Concentration; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D014151:Anti-Anxiety Agents; D002065:Buspirone