title: Feasibility of imaging pentose cycle glucose metabolism in gliomas with PET: studies in rat brain tumor models.
abstract: The feasibility of imaging pentose cycle (PC) glucose utilization in human gliomas with PET was explored in two rat glioma models by means of glucose radiolabeled in either the carbon-1 (C-1) or carbon-6 (C-6) position.
  
  
  METHODS
  In vitro, monolayers of T-36B-10 glioma, tissue slices of intracerebral glioma grafts or slices of normal brain were fed [1-14C]glucose or [6-14C]glucose, and the generated [14C]CO2 was trapped to quantitate the ratio of [14C]CO2 from 14C-1 versus 14C-6. In vivo, rats bearing grafts of either T-36B-10 or T-C6 rat gliomas at six subcutaneous sites received simultaneous intravenous injections of either [1-11C]glucose and [6-14C]glucose, or [1-14C]glucose and [6-11C]glucose. Tumors were excised between 5 and 55 min postinjection to quantify tracer uptake while arterial plasma was collected to derive time-activity input curves.
  
  
  RESULTS
  In vitro, the C-1/C-6 ratio for CO2 production from T-36B-10 monolayers was 8.8 +/- 0.4 (s.d.), in glioma slices it was 6.1 +/- 2.1 and in normal brain slices it was 1.1 +/- 0.7. PC metabolism in T-36B-10 was 1.8% +/- 0.5 of total glucose utilization. In vivo, tumor radioactivity levels normalized by plasma isotopic glucose levels showed that retained C-1 relative to C-6 radiolabeled glucose was significantly lower in both gliomas, 4.9% lower in T-36B-10 (p < 0.01) and 4.7% lower in T-C6 (p < 0.01). In an additional group of rats bearing T-36B-10 gliomas and exposed to 10 Gy of 137Cs irradiation 4 hr before isotope injection, the C-1 level was 5.6% lower than that for C-6 (p < 0.05). These results were analyzed with a model of glucose metabolism that simultaneously optimized parameters for C-1 and C-6 glucose kinetics by simulating the C-1 and C-6 tumor time-activity curves. The rate constant for loss of radiolabeled carbon from the tumors, k4, was higher for C-1 than for C-6 in all groups of rats (19% higher for T-36B-10 unirradiated, 32% for T-36B-10 irradiated and 32% for T-C6 unirradiated).
  
  
  CONCLUSIONS
  Mathematical modeling, Monte Carlo simulations and construction of receiver-operator-characteristic curves show that if human gliomas have a similar fractional use of the PC, it should be measurable with PET using sequential studies with [1-11C]glucose and [6-11C]glucose.
authors.affiliation: Department of Neurology, University of Washington School of Medicine, Seattle, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Spence
authors.forename: M M
authors.initials: MM
authors.lastname: Graham
authors.forename: M
authors.initials: M
authors.lastname: Muzi
authors.forename: S D
authors.initials: SD
authors.lastname: Freeman
authors.forename: J M
authors.initials: JM
authors.lastname: Link
authors.forename: J R
authors.initials: JR
authors.lastname: Grierson
authors.forename: F
authors.initials: F
authors.lastname: O'Sullivan
authors.forename: D
authors.initials: D
authors.lastname: Stein
authors.forename: G L
authors.initials: GL
authors.lastname: Abbott
authors.forename: K A
authors.initials: KA
authors.lastname: Krohn
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D002250:Carbon Radioisotopes; D005910:Glioma / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D012372:ROC Curve; D051381:Rats; D011916:Rats, Inbred F344; D011920:Rats, Inbred WF; D014055:Tomography, Emission-Computed; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D005947:Glucose