title: Endotracheal suctioning of the neonate: comparison of two methods as a source of mucus material for research.
abstract: Endotracheal suctioning in the neonatal intensive care setting is a routine procedure performed to maintain patency of the airway in ventilated infants. Harvested material can also be a source of mucus for research into neonatal respiratory disorders. We aimed to investigate whether the composition of material obtained by our clinically preferred technique of dry shallow suctioning differed significantly from that obtained with saline lavage and deep suctioning. Eleven pairs of dry and saline lavage aspiration samples were compared for neutrophil enzyme myeloperoxidase, total and active alpha(1)-antitrypsin, alpha(1)-antitrypsin complexed with elastase, and secretory leukoprotease inhibitor. Even though individual values of each analyte, expressed per gram of albumin, varied over at least a fivefold range, there was no difference between mean values of dry and lavage samples for any of the constituents. We conclude that the yield of material for research obtained by dry shallow suctioning is adequate and the quality at least as satisfactory as that provided by saline lavage.
authors.affiliation: Department of Paediatrics, Christchurch School of Medicine, Christchurch Hospital, New Zealand.
authors.forename: B A
authors.initials: BA
authors.lastname: Darlow
authors.forename: K B
authors.initials: KB
authors.lastname: Sluis
authors.forename: T E
authors.initials: TE
authors.lastname: Inder
authors.forename: C C
authors.initials: CC
authors.lastname: Winterbourn
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007442:Intubation, Intratracheal; D008565:Membrane Proteins / Q000032:analysis; D009093:Mucus / Q000737:chemistry; D009195:Peroxidase / Q000032:analysis; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000032:analysis; D015842:Serine Proteinase Inhibitors / Q000032:analysis; D013048:Specimen Handling / Q000379:methods; D013396:Suction / Q000379:methods*; D000515:alpha 1-Antitrypsin / Q000032:analysis
chemical_list: D008565:Membrane Proteins; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors; D000515:alpha 1-Antitrypsin; D009195:Peroxidase