p53 and Bax activation in 6-hydroxydopamine-induced apoptosis in PC12 cells.
p53, Bax and Bcl-xL proteins have been implicated in apoptotic neuronal cell death. We have investigated whether those proteins are involved in 6-OHDA-induced PC12 cell death. After a 24-h exposure to the neurotoxin (100 microM), morphological evidence for apoptosis was observed in PC12 cells. Up-regulation of p53 and Bax proteins was demonstrated 4 and 6 h, respectively, after 6-OHDA treatment; in contrast, no change in Bcl-xL levels was found. These findings suggest that p53 and Bax could be relevant markers of neuronal apoptosis as previously described in kainic acid- or ischemia-induced neuronal cell death and may participate to neuronal degeneration in Parkinson's disease.
Blum
D
D
INSERM U318, Neurobiologie Préclinique, CHU Michallon, Grenoble, France.
Wu
Y
Y
Nissou
M F
MF
Arnaud
S
S
Alim-Louis-Benabid
Verna
J M
JM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001562:Benzimidazoles; D002470:Cell Survival / Q000187:drug effects; D004586:Electrophoresis; D005456:Fluorescent Dyes; D016627:Oxidopamine / Q000494:pharmacology; D016716:PC12 Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D013565:Sympatholytics / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D001562:Benzimidazoles; D005456:Fluorescent Dyes; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D013565:Sympatholytics; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D016627:Oxidopamine; C017807:bisbenzimide ethoxide trihydrochloride