title: Role of interleukin-8 in onset of the immune response in intravesical BCG therapy for superficial bladder cancer.
abstract: In intravesical therapy for superficial bladder carcinoma urothelial cells may, through the production of cytokines, contribute to the bacillus Calmette-Guerin (BCG)-induced local immunological reaction and associated antitumor efficacy. The aim of this study was to investigate such a role for the neutrophil-attracting cytokine interleukin-8 (IL-8). The appearance of IL-8 in patients urine after BCG therapy was compared with BCG-induced IL-6 and IL-2 and the stability of IL-8 in urine was tested. Compared to IL-6 and IL-2, a rapid induction of IL-8 was observed, occurring after the first BCG instillation. Urinary IL-8 was highly stable, even after 24 h incubation at 37 degrees C. The IL-8 concentration after the first instillation seemed to be associated with subsequent development of an immune response. Consequently, IL-8 seems an attractive candidate for investigation of its prognostic value for a clinical response to BCG therapy.
authors.affiliation: Department of Urology, University of Amsterdam, The Netherlands.
authors.forename: E C
authors.initials: EC
authors.lastname: de Boer
authors.forename: L
authors.initials: L
authors.lastname: Somogyi
authors.forename: G J
authors.initials: GJ
authors.lastname: de Ruiter
authors.forename: T M
authors.initials: TM
authors.lastname: de Reijke
authors.forename: K H
authors.initials: KH
authors.lastname: Kurth
authors.forename: D H
authors.initials: DH
authors.lastname: Schamhart
mesh_terms: D000283:Administration, Intravesical; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000627:therapeutic use; D002295:Carcinoma, Transitional Cell / Q000628:therapy / Q000652:urine*; D006801:Humans; D007376:Interleukin-2 / Q000652:urine; D015850:Interleukin-6 / Q000652:urine; D016209:Interleukin-8 / Q000737:chemistry / Q000502:physiology* / Q000652:urine; D011379:Prognosis; D013997:Time Factors; D001749:Urinary Bladder Neoplasms / Q000628:therapy / Q000652:urine*
chemical_list: D001500:BCG Vaccine; D007376:Interleukin-2; D015850:Interleukin-6; D016209:Interleukin-8