Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins.
The use of lucigenin-enhanced chemiluminescence (CL) for the detection of superoxide (O2.-) has grown in popularity due to an increased demand for a simple and specific system capable of measuring superoxide. In this study we report a lucigenin-CL signal emanating from human saphenous veins (SV) that was not inhibited by superoxide dismutase (SOD) and lasted for more than 24 h. A larger CL-signal with similar properties was produced by saphenous veins that had been dehydrated. A similar, non-SOD-inhibitable lucigenin-CL was also produced with a variety of phospholipids and phosphatidic acid. The chemical moiety responsible for the phospholipid CL is oxygen dependent but remains unidentified because a variety of lipids and phosphate containing species failed to produce such a signal. These results suggest that the use of lucigenin as a specific CL enhancer for O2.- must be clearly discriminated with a specific O2.- inhibitor when used in biological systems.
Barber
D A
DA
Department of Pharmacology and Toxicology, University of Georgia, College of Pharmacy, Athens 30602-2356, USA.
Do
N H
NH
Tackett
R L
RL
Capomacchia
A C
AC
D000166:Acridines; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D015335:Molecular Probes; D010743:Phospholipids / Q000378:metabolism*; D012501:Saphenous Vein / Q000187:drug effects / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*
D000166:Acridines; D005609:Free Radicals; D015335:Molecular Probes; D010743:Phospholipids; D013481:Superoxides; C033472:10,10'-dimethyl-9,9'-biacridinium; D013482:Superoxide Dismutase