[Evaluation of pharmacopoeia methods for determination of antimicrobial agents, especially of natural substances].
Microbiological assays referring to antibiotics were first mentioned in 1955 in the US Pharmacopedia XV and in the Pharmacopedia of India I. In the pharmacopedias two general methods are employed: The first group of methods is grounded on diffusion (disc assay, cylinder-plate and hole-plate assay), the second one is based on the determination of optical density (turbidimetric method). Both methods involve certain problems, particularly with respect to the testing of low-active natural substances. In the course of our investigations those test methods were evaluated and the advantages and disadvantages discussed. The methods were compared to test systems not being described in the pharmacopedias (microdilution test, bioautographic TLC assay). In addition to that we examined spectrophotometrically (OD580nm) the influence of an antimicrobial substance and of a solutizer on the bacterial growth. Tetracycline hydrochloride was used as reference and naringenin as test substance. The results were analyzed with statistical methods cited in the pharmacopedias, e.g. straight-line method and compared to other common methods, e.g. analysis of variance.
Brantner
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Institut für Pharmakognosie der Universität Graz, Austria.
D000900:Anti-Bacterial Agents / Q000032:analysis; D000890:Anti-Infective Agents / Q000032:analysis*; D001345:Autoradiography; D002855:Chromatography, Thin Layer; D005069:Evaluation Studies as Topic; D005779:Immunodiffusion; D007201:Indicator Dilution Techniques; D009391:Nephelometry and Turbidimetry; D010602:Pharmacopoeias as Topic; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents
Bewertung von Arzneibuchmethoden zur Bestimmung der antimikrobiellen Wirksamkeit unter besonderer Berücksichtigung von Naturstoffen.