HLA-B*27 typing by PCR-restriction fragment length polymorphism.
In this study we describe the use of PCR-RFLP for genotyping HLA-B*27. A 557 bp fragment from HLA-B locus is amplified and subjected to digestion with StyI. The presence of B*27 is detected on electrophoresis by the appearance of 431 + 126 bp pattern. The same pattern could be obtained only for the very infrequent allele B*7301. However, this allele was not amplified in the B73 sample tested with the primers and conditions used in this study. Nevertheless, we have designed two PCR-RFLP approaches for separating these alleles. The PCR-RFLP method was tested on a panel of forty-three cell lines and applied to fifty spondyloarthritic patients and one-hundred-eighty healthy subjects. Given its robustness, technical simplicity and cost-effectiveness, we think that this method can be incorporated for routine use in most laboratories.
Nieto
A
A
Instituto de Parasitologia y Biomedicina Lopez Neyra, CSIC, Granada, Spain.
Fraile
A
A
Vinasco
J
J
Martín
J
J
D002460:Cell Line; D005838:Genotype; D015796:HLA-B27 Antigen / Q000145:classification* / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D015796:HLA-B27 Antigen