Nimodipine improves kainic acid induced neurotoxicity in cerebellar granular cell culture: a double-blind dose-response study.
The neuroprotective role of nimodipine was tested in kainic acid (50 and 100 microM) induced neurotoxicity in cerebellar granular cell cultures of 4 to 7 day-old rat pups. Nimodipine was applied in 50, 100 and 200 microM concentrations. Kainate, in either dose, induced cerebellar granular cell death in respect to controls and the results were statistically significant (P = 0.000 for both doses). However, kainic acid in 100 microM concentration led to higher rates of cell death than 50 microM (P = 0.017). The neuroprotective role of nimodipine in kainate induced neurotoxicity was dose dependent. Kainate toxicity in 50 microM concentration was blocked by 50 and 100 microM nimodipine concentrations (P = 0.006 and P = 0.002, respectively) while 200 microM nimodipine was found ineffective. The most effective nimodipine dose for 100 microM kainic acid neurotoxicity was 200 microM (P = 0.000) while 50 and 100 microM concentrations of nimodipine were found ineffective. In this study, we have proven the dose-dependent neuroprotective role of nimodipine in kainate induced neurotoxicity in cerebellar granular cell cultures of rat pups.
Gepdiremen
A
A
Department of Pharmacology, Atatürk University, Medical Faculty, Erzurum, Turkey.
Sönmez
S
S
Batat
I
I
Eşrefoğlu
M
M
Düzenli
S
S
Süleyman
H
H
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D004311:Double-Blind Method; D007608:Kainic Acid / Q000037:antagonists & inhibitors*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009498:Neurotoxins / Q000037:antagonists & inhibitors*; D009553:Nimodipine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D015220:Calcium Channels; D018696:Neuroprotective Agents; D009498:Neurotoxins; D009553:Nimodipine; D007608:Kainic Acid