title: Epithelium-fibroblast co-culture for assessing mucosal irritancy of metals used in dentistry.
abstract: No valid animal or in vitro model exists to assess the potential mucosal irritancy of dental materials. However, recently, a commercially available model system based on a recombined co-culture of human fibroblasts and human epithelial cells has been introduced for evaluating the time-dependent irritancy of cosmetic products. Cell viability and prostaglandin E2 (PGE2) release from the cells were used as markers for the irritative potential of test materials. The objective of the present study was to evaluate the suitability of this model for monitoring the irritative potential of metals and cast alloys used in dentistry. The human fibroblast-keratinocyte co-cultures were exposed to test specimens fabricated from copper, zinc, palladium, nickel, tin, cobalt, indium, a high noble cast alloy, and from a dental ceramic. Cell survival rates decreased after exposure to copper (14-25%), cobalt (60%), zinc (63%), indium (85%), nickel (87%), and the non-oxidized and oxidized high noble cast alloy (87%/90%) compared to untreated control cultures. Dental ceramic, palladium and tin did not influence cell viability. In parallel, the PGE2 release was continuously monitored up to 24 h using a competitive displacement enzyme immunoassay. PGE2 release increased most highly in the cultures exposed to copper (6-25 fold), cobalt (7 fold), indium (4 fold), and zinc (2 fold) compared to untreated control cultures. The PGE2 determination proved to be a non-destructive method for continuous monitoring of cell reactions in the same culture. The model used seems promising for evaluating the time-dependent mucosal irritancy of dental cast alloys.
authors.affiliation: Department of Operative Dentistry and Periodontology, University of Regenburg, Germany. Gottfried.Schmalz@klinik.uni-regensburg.de
authors.forename: G
authors.initials: G
authors.lastname: Schmalz
authors.forename: D
authors.initials: D
authors.lastname: Arenholt-Bindslev
authors.forename: K A
authors.initials: KA
authors.lastname: Hiller
authors.forename: H
authors.initials: H
authors.lastname: Schweikl
mesh_terms: D015415:Biomarkers; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000009:adverse effects; D003035:Cobalt / Q000009:adverse effects; D018920:Coculture Techniques; D003300:Copper / Q000009:adverse effects; D003722:Dental Alloys / Q000009:adverse effects*; D003764:Dental Materials / Q000009:adverse effects*; D015232:Dinoprostone / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005240:Feasibility Studies; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006047:Gold Alloys / Q000009:adverse effects; D006801:Humans; D007124:Immunoenzyme Techniques; D007204:Indium / Q000009:adverse effects; D007509:Irritants / Q000009:adverse effects*; D008670:Metals / Q000009:adverse effects*; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009532:Nickel / Q000009:adverse effects; D010165:Palladium / Q000009:adverse effects; D013997:Time Factors; D014001:Tin / Q000009:adverse effects; D015032:Zinc / Q000009:adverse effects
chemical_list: D015415:Biomarkers; D003722:Dental Alloys; D003764:Dental Materials; D006047:Gold Alloys; D007509:Irritants; D008670:Metals; D007204:Indium; D003035:Cobalt; D010165:Palladium; D014001:Tin; D003300:Copper; D009532:Nickel; D015032:Zinc; D015232:Dinoprostone