[The inhibitory effect of LTB4 antagonist on eosinophil infiltration in cutaneous and gut late phase response in BALB/C mice sensitized with ovalubumin (OVA)].
In this study we investigated the effect of LTB4 antagonist on eosinophil infiltration in skin and gut late phase response (LPR) in OVA-sensitized BALB/c mice. The eosinophil infiltrations to skin and gut induced by skin and oral challenge reached a peak at 12 h and 6 h after the challenge, respectively. Intraperitoneal administration of LTB4 antagonist (ONO-4057) before the challenge significantly inhibited eosinophil infiltrations to the skin and gut by 53.3% and 73.7%, respectively (p < 0.05). Next, we investigated the effect to that by PAF antagonist (ONO-6240) and anti-IL-5 mAb in the skin system. OVA-induced eosinophil infiltration at 12 h after intracutaneous challenge was significantly inhibited by peritoneal administration of anti-IL-5 mAb before the challenge by 89.6% (p < 0.05), but not by that of PAF antagonist. Our results demonstrated the inhibitory effect of LTB4 antagonist on eosinophil infiltration in skin and gut LPR, suggesting the potency of LTB4 antagonist for treatment of skin lesion and food allergy in atopic dermatitis considered to be associated with LPR.
Hakugawa
J
J
Department of Dermatology, Nagasaki University School of Medicine.
Bae
S J
SJ
Vinuesa
M A
MA
Tanaka
Y
Y
Katayama
I
I
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D003876:Dermatitis, Atopic / Q000473:pathology; D004804:Eosinophils / Q000276:immunology / Q000473:pathology*; D005260:Female; D007114:Immunization; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007422:Intestines / Q000473:pathology*; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D010666:Phenylpropionates / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D012867:Skin / Q000473:pathology*; D013844:Thiazoles / Q000494:pharmacology
D007166:Immunosuppressive Agents; D010666:Phenylpropionates; D010972:Platelet Activating Factor; D013844:Thiazoles; C067523:ONO-LB 457; D007975:Leukotriene B4; D010047:Ovalbumin; C048549:ONO 6240