Observation of non-covalent interactions between beauvericin and oligonucleotides using electrospray ionization mass spectrometry.
Electrospray ionization mass spectrometry has been used to study the possible non-covalent interaction between oligonucleotides and beauvericin (B) mycotoxin. Beauvericin-oligonucleotide adduct formation was observed even at low mycotoxin concentration (25 pmol/microL). Adducts were found with different numbers of B ligands attached. The selectivity of binding of B ligands to two different oligonucleotides has been shown to be similar indicating that beauvericin does not have a strongly preferred base sequence or base site in the DNA. In a competitive complexation reaction, beauvericin forms specific adducts with oligonucleotide while another mycotoxin, nigeromicin, which causes apoptosis without fragmentation of DNA, does not.
Pócsfalvi
G
G
Centro Internazionale di Servizi di Spettrometria di Massa del C.N.R., Napoli, Italy.
Di Landa
G
G
Ferranti
P
P
Ritieni
A
A
Randazzo
G
G
Malorni
A
A
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D053938:DNA Fragmentation / Q000187:drug effects; D004271:DNA, Fungal / Q000032:analysis; D047630:Depsipeptides; D005285:Fermentation; D005670:Fusarium / Q000378:metabolism; D013058:Mass Spectrometry; D009841:Oligonucleotides / Q000737:chemistry*; D010455:Peptides
D000900:Anti-Bacterial Agents; D004271:DNA, Fungal; D047630:Depsipeptides; D009841:Oligonucleotides; D010455:Peptides; C004456:beauvericin