Rapid detection of bacterial surface proteins using an enzyme-linked immunosorbent assay system.
In this communication a rapid and simple enzyme-linked immunosorbent assay is described, suitable for the investigation of bacterial surface proteins. The protocol uses whole cells and avoids any centrifugation or cross-linking steps. Less than 10(6) cells were detected by this procedure.
Burkovski
A
A
Forschungszentrum Jülich, Institut für Biotechnologie 1, Germany. A.Burkovski@kfajuelich.de
D018528:ATP-Binding Cassette Transporters / Q000032:analysis / Q000276:immunology; D027322:Amino Acid Transport System X-AG; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins / Q000032:analysis*; D015169:Colony Count, Microbial; D003352:Corynebacterium / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D008565:Membrane Proteins / Q000032:analysis*
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins