Endothelin B receptor-like immunoreactivity is associated with LHRH-immunoreactive fibers in the rat hypothalamus.
Antisera were raised against amino-acid residues 425-439 of the rat endothelin B (ETB) receptor. Massive ETB receptor immunoreactive fibers were observed in the median eminence and organum vasculosum lamina terminalis of rats. Double labeling studies with anti-luteinizing hormone-releasing hormone (LHRH) antiserum revealed that ETB receptor-like immunoreactivity associated with LHRH-immunoreactive fibers in these areas. However, ETB receptor-like immunoreactivity was not detected in LHRH-immunoreactive somata. This result suggests that endothelin affects the LHRH neuronal systems via ETB receptors on the axon at the terminal field.
Yamamoto
T
T
Department of Biology, Kanagawa Dental College, Yokosuka, Japan.
Suzuki
H
H
Uemura
H
H
D000818:Animals; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D006168:Guinea Pigs; D007031:Hypothalamus / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008473:Median Eminence / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009412:Nerve Fibers / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000378:metabolism*; D014018:Tissue Distribution
D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin; D007987:Gonadotropin-Releasing Hormone