Interleukin (IL) 4, the cytokine that isn't there: reactivity of IL-4 antibodies with cells in IL-4 -/- mice.
We analyzed cytokine expression in recipient spleens and cardiac allografts placed in mice that were unable to synthesize interleukin (IL)-4 due to disruption of the IL-4 gene (IL-4 -/-) and in wild-type (IL-4 +/+) mice. Polyclonal BL-4P and monoclonal 11B11, 1D11, and 24G2 anti-IL-4 antibodies were used to detect cell-surface and cytoplasmic antigens in sections of frozen tissue. All of the antibodies were found to react with non-IL-4 determinants associated with graft-infiltrating cells, and BL-4P, 1D11, and 24G2 bound to cells and connective tissue in the spleens of IL-4 -/- mice. The IL-4-producing cell line, X63Ag8-653 (X63), was used as a positive control for IL-4 staining and to test the ability of recombinant IL-4 to block the binding of antibodies to IL-4.
Mottram
P L
PL
Department of Surgery, Royal Melbourne Hospital, Victoria, Australia.
Purcell
L J
LJ
Han
W R
WR
Maguire
J
J
Stein-Oakley
A N
AN
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015704:CD4 Antigens / Q000276:immunology; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D006085:Graft Survival / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology*; D007124:Immunoenzyme Techniques; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000172:deficiency / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology*; D014184:Transplantation, Homologous
D000906:Antibodies; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016207:Cytokines; D015847:Interleukin-4