Chicken egg yolk antibodies against F18ab fimbriae of Escherichia coli inhibit shedding of F18 positive E. coli by experimentally infected pigs.
F18ab and F18ac are antigenic variants of a colonizing fimbria commonly found on E. coli associated with postweaning diarrhea and edema disease in pigs. Chicken F18ab antibodies were obtained by immunising hens with purified F18ab fimbriae. For their in vitro characterisation antibodies were isolated from diluted egg yolks by ammonium sulfate precipitation. In vitro adhesion tests demonstrated that the chicken F18ab antibodies inhibited attachment of F18ab positive E. coli bacteria to the intestinal mucosa. Just weaned piglets were experimentally infected with an F18ab positive edema disease strain of E. coli, or with an F18ac positive postweaning diarrhea E. coli strain. The animals were infected on the second day of a period during which chicken F18ab antibodies were added to their feed. During the same period, pigs of the control group received commercial eggs in which no F18 antibodies were detected. In both experimental infections the excretion of the F18 positive strain was reduced in pigs that received the F18ab antibodies as compared to the control animals. The F18ab antibodies diminished the cases of diarrhea and death in animals infected with F18ac positive E. coli.
Imberechts
H
H
Laboratory of Bacteriology, National Institute for Veterinary Research, Brussels, Belgium.
Deprez
P
P
Van Driessche
E
E
Pohl
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000627:therapeutic use*; D002645:Chickens; D003967:Diarrhea / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D004487:Edema / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D004530:Egg Yolk / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology* / Q000517:prevention & control; D010861:Fimbriae, Bacterial / Q000276:immunology*; D007116:Immunization, Passive / Q000379:methods / Q000662:veterinary*; D013552:Swine
D000907:Antibodies, Bacterial