The recA gene from Clostridium perfringens is induced by methyl methanesulphonate and contains an upstream Cheo box.
The recA gene from Clostridium perfringens was cloned using degenerate oligonucleotide primers designed from conserved regions of RecA proteins from other bacteria. The 1089 bp gene encoded a putative RecA protein with 69% amino acid sequence similarity to the RecA protein from Bacillus subtilis. The C. perfringens recA gene was induced by exposure to methyl methanesulphonate and complemented a recA mutant of Escherichia coli. A cheo box was identified in the region upstream of the gene. Since this SOS-like operator site is conserved in many DNA-damage-inducible recA gene regions from Gram-positive bacteria, the results suggest that the regulation of the C. perfringens recA gene also involves the binding of a LexA-like protein to this site.
Johnston
Joanne L
JL
Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
Sloan
Joan
J
Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
Fyfe
Janet A M
JAM
Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
Davies
John K
JK
Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
Rood
Julian I
JI
Department of Microbiology, Monash University, Clayton, Victoria 3168, Australia.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D011938:Rec A Recombinases / Q000235:genetics*; D017421:Sequence Analysis; D013450:Sulfones / Q000494:pharmacology*
D013450:Sulfones; D008741:Methyl Methanesulfonate; D011938:Rec A Recombinases