Rainbow Universal CPG: a versatile solid support for oligonucleotide synthesis.
We have developed a universal solid support, termed Rainbow Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore glass (CPG) support. Deprotection of oligodeoxyribonucleotides was optimized using 0.5 M LiCl in concentrated ammonium hydroxide. PCR experiments using three different sets of primers proved that the 3'-hydroxyl function of oligodeoxyribonucleotides synthesized from Rainbow Universal CPG was retained. This universal solid support shows promise for replacing the standard nucleoside CPG supports.
Nelson
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CLONTECH Laboratories, Inc., Palo Alto, CA, USA.
Muthini
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Vierra
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Acosta
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Smith
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D001331:Automation; D001710:Biotin; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D005898:Glass; D006801:Humans; D018021:Lithium Chloride; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D010920:Placenta / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013696:Temperature; D013698:Templates, Genetic
D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D001710:Biotin; D018021:Lithium Chloride