title: Characterization of a new ribotoxin gene (c-sar) from Aspergillus clavatus.
abstract: A new ribotoxin, c-sarcin, was isolated from a culture of Aspergillus clavatus. A full-length genomic DNA (c-sar) coding for c-sarcin was cloned and sequenced. The deduced amino acid sequence showed a high homology to restrictocin and alpha-sarcin. The native toxin as well as the recombinant protein hydrolysed ribosomes and naked RNA. The genomic structure of the c-sar gene had an intron located between the coding sequences for secretory signal peptide and the mature protein. The intron contained a stretch of 38 adenines. The intron sequence of c-sar was different from that of restrictocin but resembled that of alpha-sarcin. There was 34% identity between the intron of c-sarcin and alpha-sarcin, and this similarity was further increased to 83% if the stretch of polyadenine was omitted.
authors.affiliation: Institute of Genetics, National Yang-Ming University, Taipei, Taiwan, R.O.C.
authors.forename: K C
authors.initials: KC
authors.lastname: Huang
authors.forename: Y Y
authors.initials: YY
authors.lastname: Hwang
authors.forename: L
authors.initials: L
authors.lastname: Hwu
authors.forename: A
authors.initials: A
authors.lastname: Lin
mesh_terms: D001230:Aspergillus / Q000235:genetics*; D001483:Base Sequence; D003603:Cytotoxins / Q000235:genetics*; D004722:Endoribonucleases / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics* / Q000302:isolation & purification*; D005784:Gene Amplification; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: C105968:C-SAR protein, Aspergillus clavatus; D003603:Cytotoxins; D005656:Fungal Proteins; C014801:alpha-sarcin; D004722:Endoribonucleases; D012260:Ribonucleases