title: Lazaroid U-74500A for warm ischemia and reperfusion injury of the canine small intestine.
abstract: BACKGROUND
  Although lazaroids have been shown to protect various organs from ischemia/reperfusion injury, results obtained in the small intestine have been conflicting.
  
  
  METHODS
  The canine small intestine was made totally ischemic for 2 hours by occluding the superior mesenteric artery and the superior mesenteric vein with interruption of the mesenteric collateral vessels. A lazaroid compound, U74500A, or a citrate vehicle was given intravenously to each of the six animals for 30 minutes before intestinal ischemia. Intestinal tissue blood flow, lipid peroxidation, neutrophil infiltration, adenine nucleotides and their catabolites, and histologic changes after reperfusion were determined.
  
  
  RESULTS
  Lazaroid treatment attenuated decline of the mucosal and serosal blood flow after reperfusion. Accumulation of lipid peroxidation products and neutrophils in mucosal tissues was markedly inhibited by the treatment. Postischemic energy resynthesis was also augmented by lazaroid. Morphologically, mucosal architectures were better preserved with lazaroid treatment after reperfusion, and recovered to normal by postoperative day 3 in the treated group and by postoperative day 7 in control animals.
  
  
  CONCLUSIONS
  Lazaroids protect the canine small intestine from ischemia/reperfusion injury by inhibiting lipid peroxidation and neutrophil infiltration. Dogs are tolerant of 2-hour normothermic complete intestinal ischemia.
authors.affiliation: Pittsburgh Transplantation Institute, University of Pittsburgh, Pa, USA.
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: N
authors.initials: N
authors.lastname: Ishizaki
authors.forename: M B
authors.initials: MB
authors.lastname: Jin
authors.forename: T
authors.initials: T
authors.lastname: Azuma
authors.forename: R
authors.initials: R
authors.lastname: Lee
authors.forename: T E
authors.initials: TE
authors.lastname: Starzl
authors.forename: S
authors.initials: S
authors.lastname: Todo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D004285:Dogs; D005260:Female; D007421:Intestine, Small / Q000098:blood supply* / Q000473:pathology; D015227:Lipid Peroxidation; D009092:Mucous Membrane / Q000473:pathology; D009504:Neutrophils / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011281:Pregnatrienes / Q000627:therapeutic use*; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000975:Antioxidants; D011281:Pregnatrienes; D009195:Peroxidase; C053356:U 74500A