Simultaneous demonstration of alkaline and acid phosphatase activity in bone, at bone-implant interfaces and at the epiphyseal growth plate in plastic-embedded undemineralized tissues.
The aim of the present study was to determine if it was possible to detect in bone, at the epiphyseal growth plate and at the bone-implant interface, the presence of alkaline and acid phosphatases using Technovit 7200 VLC resin. In the plastic-embedded specimens it was possible to observe the simultaneous presence of acid and alkaline phosphatases at the epiphyseal growth plate in the presence of intra-articular implants. The morphology of the cells positive for the phosphatases was very clear, with no apparent diffusion of the reaction product and no sputter ground staining.
Piattelli
A
A
Dental School, University of Chieti, Italy.
Piattelli
M
M
Scarano
A
A
D000135:Acid Phosphatase / Q000032:analysis*; D000180:Acrylic Resins; D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D001672:Biocompatible Materials; D001860:Bone Plates; D005269:Femur; D006132:Growth Plate / Q000201:enzymology*; D008297:Male; D010969:Plastics; D011817:Rabbits; D014025:Titanium
D000180:Acrylic Resins; D001672:Biocompatible Materials; D010969:Plastics; D014025:Titanium; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase