title: alpha-CGRP mRNA levels in motoneurons innervating specific rat muscles.
abstract: Along with acetylcholine, motoneurons express several neuromodulatory peptides. The most extensively studied of these peptides is calcitonin gene-related peptide (CGRP). CGRP modulates the biochemical, physiological and metabolic properties of skeletal muscle primarily through activation of membrane receptors. Virtually all motor pool contain motoneurons that are immunoreactive for CGRP. The purpose of this study was to determine the proportions of motoneurons that express alpha-CGRP in motor pools innervating muscles with different motor unit compositions. These include the soleus, extensor digitorum longus, tensor fascia latae and the diaphragm muscles as well as the spinal nucleus of the bulbocavernosus. The spinal nucleus of the bulbocavernosus provides innervation to the bulbocavernosus/levator ani muscle complex and the external anal sphincter muscle. The spinal nucleus of the bulbocavernosus contained the greatest proportion of alpha-CGRP mRNA-positive motoneurons, followed in descending rank order by the tensor fascia latae, the extensor digitorum longus, the soleus and the diaphragm motor pools. In addition, significant differences between motor pools were observed in the mean relative alpha-CGRP mRNA level among those motoneurons expressing alpha-CGRP. The highest mean relative alpha-CGRP mRNA level was observed in soleus and the extensor digitorum longus motor pools; followed in descending rank order by the tensor fascia latae, the diaphragm and the spinal nucleus of the bulbocavernosus. We have previously shown that muscle contractile inactivity increases the number of motoneurons that express alpha-CGRP and in the relative mRNA levels. The results of the present study suggest that the proportion of motoneurons within a motor pool that express alpha-CGRP may be closely related to the contractile activity (i.e. activation history) of the target muscle.
authors.affiliation: Department of Neurobiology, University of California at Los Angeles School of Medicine, 90024, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Blanco
authors.forename: P
authors.initials: P
authors.lastname: Popper
authors.forename: P
authors.initials: P
authors.lastname: Micevych
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics*; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000294:innervation*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
chemical_list: D012333:RNA, Messenger; D015740:Calcitonin Gene-Related Peptide