title: Two new regulatory elements controlling the Drosophila Sgs-3 gene are potential ecdysone receptor and fork head binding sites.
abstract: We identified two regulatory elements in the upstream region of the Drosophila Sgs-3 gene which are both able to bind the ecdysone receptor (EcR/USP) and the product of the fork head gene. Interestingly, only one of the EcR/USP binding sites is able to recognize in vitro-translated EcR/USP, which provides evidence for the existence of different receptor forms having different DNA binding specificities. Deletions of the elements lead to a reduced accumulation of Sgs-3 mRNA without altering the temporal expression profile of the gene. The data are consistent with the hypothesis that the ecdysone receptor directly contributes to the transcriptional activation of Sgs-3 by binding to at least one of the two elements. Since also the Sgs-4 gene is controlled by a functional EcR/USP binding site, a direct participation of EcR/USP in the formation of regulatory complexes may be of general importance for the hormonal control of Sgs genes.
authors.affiliation: Institut für Genetik der Freien Universität Berlin, Germany. mlehm@genetik.biologie.fu-berlin.de
authors.forename: M
authors.initials: M
authors.lastname: Lehmann
authors.forename: F
authors.initials: F
authors.lastname: Wattler
authors.forename: G
authors.initials: G
authors.lastname: Korge
mesh_terms: D000818:Animals; D001665:Binding Sites; D004330:Drosophila / Q000235:genetics*; D004440:Ecdysone / Q000378:metabolism; D051858:Forkhead Transcription Factors; D005967:Glue Proteins, Drosophila / Q000235:genetics*; D007301:Insect Hormones / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D051858:Forkhead Transcription Factors; D005967:Glue Proteins, Drosophila; D007301:Insect Hormones; D009687:Nuclear Proteins; D011987:Receptors, Steroid; D014157:Transcription Factors; C055469:ecdysone receptor; D004440:Ecdysone