[Evaluation of micronuclei in primary hepatocyte culture in rats treated with organophosphoric compounds].
We have evaluated the genotoxic effect of the three organophosphorous (OPs) insecticides dimethoate (DIM), azinphosmethyl (AZN) and diazinon (DIA) or in combination, at a ratio frequently found in foodstuffs by residual analysis. We have carried out the micronucleus (MN) test on cultured rat hepatocytes which were induced to proliferate by epidermal growth factor (EGF), and treated with the insecticides for 48 hours. The doses tested were chosen on the basis of cytotoxicity tests such as Trypan Blue exclusion and mitotic index inhibition. The results showed that AZN was the most toxic and DIM the least toxic compound. None of the compounds tested showed a genotoxic effect when examined separately. Conversely, a significant increase in MN frequency was observed when the three OPs were used in combination at the lowest concentration used when each compound was tested alone.
Frölichsthal
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Laboratorio di Neurofarmacologia Sprimentale, Istituto Nazionale Neurologico C. Besta, Milano.
Piatti
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D000818:Animals; D002478:Cells, Cultured; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009943:Organophosphorus Compounds / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
D009153:Mutagens; D009943:Organophosphorus Compounds
Valutazione dei micronuclei in colture primarie di epatociti di ratto dopo trattamento con composti organofosforici.