HLA-A locus DNA typing of the 4AOH cell panel by arms PCR.
As part of the Fourth Asia-Oceania Histocompatibility (4AOH) Workshop, the authors have demonstrated a method of DNA-based tissue typing of the HLA-A locus using ARMS-designed primers in a panel of specific PCR reactions. The study was carried out blind under Workshop conditions and the results confirm the method as an accurate means of determining HLA-A locus tissue types.
Krausa
P
P
Imperial Cancer Research Fund, Institute of Molecular, Medicine, John Radcliffe Hospital, Oxford, UK.
Browning
M J
MJ
Moses
J H
JH
Bodmer
J G
JG
D002874:Chromosome Mapping; D004247:DNA / Q000145:classification; D005240:Feasibility Studies; D015234:HLA-A Antigens / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods
D015234:HLA-A Antigens; D004247:DNA