title: Effects of reducing reagents and temperature on conversion of nitrite and nitrate to nitric oxide and detection of NO by chemiluminescence.
abstract: To measure the concentration of nitrites and nitrates by chemiluminescence, we examined the efficiency of five reducing agents [V(III), Mo(VI) + Fe(II), NaI, Ti(III), and Cr(III)] to reduce nitrite (NO2-) and (or) nitrate (NO3-) to nitric oxide (NO). The effect of each reducing agent on the conversion of different amounts of NO2- and (or) NO3- (100-500 pmol, representing concentrations of 0.4 to 2 mu molar) to NO was determined at 20 degrees C for NO2- and at 80 degrees C for NO3-. The effect of temperature from 20 to 90 degrees C on the conversion of a fixed amount of NO2- or NO3- (400 pmol or 1.6 mu molar) to NO was also determined. These five reducing agents are similarly efficient for the conversion of NO2- to NO at 20 degrees C. V(III) and Mo(VI) + Fe(II) can completely reduce NO3- to NO at 80 degrees C. NaI and Cr(III) were unable to convert NO3- to NO. Increased temperature facilitated the conversion of NO3- to NO, rather than that of NO2- to NO. We evaluated the recovery of NO2- and NO3- from plasmas of pig and of dog. Recovery from plasma of both animals was reproducible and near quantitative.
authors.affiliation: Department of Anesthesia, Hôpital Notre-Dame, Université de Montréal, QC, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.forename: E
authors.initials: E
authors.lastname: Troncy
authors.forename: M
authors.initials: M
authors.lastname: Francoeur
authors.forename: B
authors.initials: B
authors.lastname: Vinet
authors.forename: P
authors.initials: P
authors.lastname: Vinay
authors.forename: G
authors.initials: G
authors.lastname: Czaika
authors.forename: G
authors.initials: G
authors.lastname: Blaise
mesh_terms: D000818:Animals; D002857:Chromium / Q000737:chemistry; D004285:Dogs; D005296:Ferrous Compounds / Q000737:chemistry; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008982:Molybdenum / Q000737:chemistry; D009566:Nitrates / Q000737:chemistry*; D009569:Nitric Oxide / Q000097:blood* / Q000737:chemistry*; D009573:Nitrites / Q000737:chemistry*; D010084:Oxidation-Reduction; D012974:Sodium Iodide / Q000737:chemistry; D013552:Swine; D013696:Temperature; D014025:Titanium / Q000737:chemistry; D014639:Vanadium / Q000737:chemistry
chemical_list: D005296:Ferrous Compounds; D007202:Indicators and Reagents; D009566:Nitrates; D009573:Nitrites; D014639:Vanadium; D002857:Chromium; D009569:Nitric Oxide; D008982:Molybdenum; D014025:Titanium; D012974:Sodium Iodide