Interleukin 12 and direct cytotoxicity of spleen lymphocytes to Listeria innocua-phagocyting syngeneic macrophages in C57BL/6 and BALB/c mice.
Production of interleukin 12 (IL-12) during cytotoxic reaction of C57BL/6 and BALB/c spleen lymphocytes or their subpopulations: T+B, T, CD4+ and CD8+ cells to L. innocua-phagocyting syngeneic macrophages was examined. The effector cell donors were untreated or L. innocua-infected. The number of surviving bacteria in phagocytes was tested and IL-12 level in culture supernatants of reacting cells was determined. C57BL/6 mice, resistant to Listeria infection, were found to develop stronger cell cytotoxicity to bacteria-phagocyting syngeneic macrophages than BALB/c mice. The lymphocytes responsible for that phenomenon were of CD8+ phenotype. IL-12 was produced only during nonspecific cytotoxic reaction of CD4+ and CD8+ T cells. It is suggested that the innate resistance of mice to Listeria is dependent on their ability to develop a specific cytotoxic reaction and IL-12 production.
Szeliga
J
J
Department of Immunology, University of Lódź, Poland.
Fiuk
D
D
Walencka
M
M
Gościcka
T
T
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005260:Female; D007113:Immunity, Innate; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000502:physiology* / Q000633:toxicity; D008087:Listeria / Q000254:growth & development / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D018664:Interleukin-12