title: Purification and characterization of a human brain galectin-1 ligand.
abstract: Our previous studies have characterized an endogenous lectin from human brain identified as galectin-1. A soluble ligand of galectin-1 was purified from human brain by affinity chromatography and preparative electrophoresis. The purified ligand (termed HBGp82, for human brain galectin-1-binding polypeptide of 82,000 daltons) has an apparent molecular mass of 82 kDa and is glycosylated by N-linked biantennary complex structures. HBGp82 was partially characterized by microsequencing of peptide fragments. Similar peptides were found in a heat shock of protein of 90,000 daltons, hsp90. However, comparison of apparent molecular weights and matrix-assisted laser desorption mass spectrometry clearly showed that HBGp82 differs to some degree from hsp90.
authors.affiliation: Laboratoire de Biochimie et Technologie des Protéines, Université Paris-Nord, Bobigny, France.
authors.forename: A
authors.initials: A
authors.lastname: Chadli
authors.forename: J P
authors.initials: JP
authors.lastname: LeCaer
authors.forename: D
authors.initials: D
authors.lastname: Bladier
authors.forename: R
authors.initials: R
authors.lastname: Joubert-Caron
authors.forename: M
authors.initials: M
authors.lastname: Caron
mesh_terms: D000595:Amino Acid Sequence; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D037483:Galectin 1; D006031:Glycosylation; D006388:Hemagglutinins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D008024:Ligands; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009479:Neuropeptides / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D037483:Galectin 1; D006388:Hemagglutinins; D037102:Lectins; D008024:Ligands; D009479:Neuropeptides