A color spot test for the detection of Kryptofix 2.2.2 in [18F]FDG preparations.
A color spot test is described that can confirm the absence of Kryptofix 2.2.2 in 2-[18F]fluoro-2-deoxy-D-glucose ([18F]FDG) in less than 5 min. Pretreated strips of plastic-backed silica gel 60 thin-layer chromatographic medium, saturated with iodoplatinate reagent, are over-spotted with separate droplets of final product [18F]FDG and Kryptofix standard solutions. A blue-black circular spot is clearly visible at Kryptofix concentrations as low as 2 micrograms/mL.
Mock
B H
BH
Department of Radiology, Indiana University Hospital, Indianapolis 46202-5253, USA. BMOCK@xray.indyrad.iupui.edu
Winkle
W
W
Vavrek
M T
MT
D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000032:analysis*; D002614:Chelating Agents / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods; D003124:Colorimetry / Q000379:methods; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000592:standards; D005462:Fluorine Radioisotopes; D019788:Fluorodeoxyglucose F18; D007202:Indicators and Reagents; D011934:Reagent Strips
D019086:Bridged Bicyclo Compounds, Heterocyclic; D002614:Chelating Agents; D005462:Fluorine Radioisotopes; D007202:Indicators and Reagents; D011934:Reagent Strips; D019788:Fluorodeoxyglucose F18; C006071:cryptating agent 222; D003847:Deoxyglucose