Interleukin-10 immunoadhesin production by a replication-defective adenovirus.
The present study examined the use of replication-defective adenovirus for in vitro production of an immunoadhesin. A recombinant adenovirus, rendered replication defective by deletion of the E1 gene, was constructed to contain the murine interleukin-10 gene fused in frame with the hinge, CH2, and CH3 domains of the murine immunoglobulin gamma 1 heavy chain constant region gene under the control of the human cytomegalovirus promoter. The resultant recombinant virus, Ad5.hCMV.mIL-10:HFc, was used to transduce several cell types. The expressed protein, mIL-10:HFc, is secreted as a disulfide-bonded homodimer. In vitro, a murine pro-B-cell line expressing transfected recombinant murine interleukin-10 receptor proliferated in response to purified mIL-10:HFc. The results obtained demonstrate the relative ease of production of an immunoadhesin using replication-defective adenovirus.
Anglade
E
E
Laboratory of Immunology, National Eye Institute, Bethesda, MD 20892, USA.
Sullivan
D M
DM
Csaky
K G
KG
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line; D003673:Defective Viruses / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis*; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection; D014779:Virus Replication / Q000235:genetics*
D015815:Cell Adhesion Molecules; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D016753:Interleukin-10