title: Emergence of an inhibitor-resistant beta-lactamase (SHV-10) derived from an SHV-5 variant.
abstract: An inhibitor-resistant beta-lactamase (SHV-10), derived from an SHV-5 variant (SHV-9), was found in an Escherichia coli clinical isolate. In SHV-10, Ser-130 was replaced by Gly. The enzyme partially retained its ability to hydrolyze penicillins, but its activity against cephalosporins was drastically reduced. A Ser-130-->Gly mutant of the prototype SHV-5, obtained by site-directed mutagenesis, had properties similar to those of SHV-10.
authors.affiliation: Department of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: E E
authors.initials: EE
authors.lastname: Prinarakis
authors.forename: V
authors.initials: V
authors.lastname: Miriagou
authors.forename: E
authors.initials: E
authors.lastname: Tzelepi
authors.forename: M
authors.initials: M
authors.lastname: Gazouli
authors.forename: L S
authors.initials: LS
authors.lastname: Tzouvelekis
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology*; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000235:genetics; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams; D001618:beta-Lactamases