Highly purified D1/D2/cyt b559 preparations from spinach do not contain the non-heme iron center.
57Fe enriched D1/D2/cyt b559 preparations were isolated from spinach grown hydroponically on a 57Fe containing medium. In terms of polypeptide and pigment composition these samples are of high purity and functional integrity of P680. Mössbauer spectra measured in D1/D2/cyt b559 complexes revealed that these preparations are completely deprived of the non-heme iron center. Possible implications of this finding are discussed for the electron transfer from Pheo-. to exogenous electron acceptors.
Kurreck
J
J
Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Technische Universität Berlin, Germany.
Garbers
A
A
Parak
F
F
Renger
G
G
D003573:Cytochrome b Group / Q000737:chemistry* / Q000302:isolation & purification; D007501:Iron / Q000032:analysis*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry; D045332:Photosystem II Protein Complex; D015204:Spectroscopy, Mossbauer; D018724:Spinacia oleracea / Q000737:chemistry*
D003573:Cytochrome b Group; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C019313:cytochrome b559; D007501:Iron