Suppressed induction of 2-5AS in cells with HTLV-I is not associated with the fluctuation of IFN-receptor, IFN-regulatory factors (IRF-1 and IRF-2) or STAT-1 alpha.
Some viruses seem to be capable of suppressing interferon (IFN)-induced 2',5'-oligoadenylate synthetase (2-5AS) induction. Cells infected with human T-lymphotropic virus type-I (HTLV-I) show different natures for the constitutive production of IFN-gamma or sensitivity to IFN. Poor induction of 2-5AS was found in IFN-gamma producer cells carrying HTLV-I (MT-1, MT-2 and SMT-1). On the other hand, in non- or low-producing cell lines of IFN-gamma such as HUT102 and OKM-2, significant activity of 2-5AS was induced by treatment with IFN-alpha. A satisfactory level of IFN receptor was detectable in SMT-1 cells in spite of the poor induction of 2-5AS. There were no differences in either the interferon regulatory factor-2 (IRF-2) mRNA transcript or the level of STAT-1 alpha between SMT-1 and HUT102 cells. However, the transcription of IRF-1 mRNA was slightly reduced in SMT-1 cells as compared with that of HUT102 cells.
Yokosawa
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N
Department of Microbiology, Sapporo University School of Medicine, Hokkaido, Japan.
Indoh
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T
Kubota
T
T
Yashiki
T
T
Fujii
N
N
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000235:genetics*; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism*; D006801:Humans; D050856:Interferon Regulatory Factor-1; D050836:Interferon Regulatory Factor-2; D008565:Membrane Proteins; D010750:Phosphoproteins / Q000235:genetics*; D012333:RNA, Messenger; D053633:Receptor, Interferon alpha-beta; D017471:Receptors, Interferon / Q000235:genetics*; D012097:Repressor Proteins; D050794:STAT1 Transcription Factor; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C494257:IRF1 protein, human; C494229:IRF2 protein, human; D050856:Interferon Regulatory Factor-1; D050836:Interferon Regulatory Factor-2; D008565:Membrane Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D017471:Receptors, Interferon; D012097:Repressor Proteins; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D053633:Receptor, Interferon alpha-beta; D015088:2',5'-Oligoadenylate Synthetase