Acute intensive interval training and in vitro t-lymphocyte function.
Five male endurance-trained runners completed an interval running session of 15 x 1-min intervals at 95% VO2 max. Venous blood samples were collected pre-exercise and then immediately, 30- and 60-minutes post-exercise. The response of cultures of total lymphocytes to mitogen (phytohaemagglutinin) were significantly reduced immediately after exercise, but returned to resting levels by 30-min of recovery. Conversely, the mitogen response of cultures of pure T-lymphocytes (CD4+ and CD8+ cells), separated using a magnetic separation technique, showed no significant change during the exercise and recovery periods. These data showed directly that there was no apparent change in the functional capability of T-lymphocytes following an intensive interval training session. Furthermore, there were significant changes in the composition of the total lymphocyte cultures immediately post-exercise; increased numbers of natural killer (NK) cells (CD56+) and T-suppressor cells (CD8+) and decreased numbers of T-helper cells (CD4+). There were also significant correlations between total mitogen response and the composition of the cultured lymphocytes. These data indicated that the large increases in NK cells, relative to T-cells, following intensive exercise, were the most likely cause of the reduced mitogen response of total lymphocyte cultures.
Hinton
J R
JR
Department of Biomedical Science, Anglia Polytechnic University, Cambridge, United Kingdom.
Rowbottom
D G
DG
Keast
D
D
Morton
A R
AR
D000328:Adult; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D017079:Exercise Tolerance; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology; D008213:Lymphocyte Activation / Q000187:drug effects; D018655:Lymphocyte Count; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D010101:Oxygen Consumption; D010807:Physical Endurance / Q000502:physiology; D010835:Phytohemagglutinins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000145:classification / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology; D013997:Time Factors
D008934:Mitogens; D010835:Phytohemagglutinins