[Evaluation of the reproducibility of the MAST technique for pneumoallergens and food allergens. Biological study].
MAST-Cla reagent is used in France for now 10 years for the specific IgE determinations. Due to the different modifications of the manufactoring process we checked here the actual sensitivity of this test as compared to the results obtained by the CAP RAST RIA. The mean sensitivities related to the class 1 limit obtained by successive dilutions of the tested sera were respectively for the major aero-allergens and food allergens 0.40 kUI/l and 0.49 kUI/l. Four major allergens (grass pollen, house dust mite, egg yolk and milk) we observed a low background for 10 control subjects, the mean values (n = 6) related to a low concentration of grass pollen specific IgE (0.5 kUl/l) being significant (p < 0.01).
Sainte-Laudy
J
J
Unité d'allergologie, laboratoire Cerba, Cergy-Pontoise.
Dewilde
F X
FX
Brouillaud
J
J
D000485:Allergens / Q000276:immunology*; D000818:Animals; D002417:Cattle; D004391:Dust; D004530:Egg Yolk / Q000276:immunology*; D005512:Food Hypersensitivity / Q000209:etiology / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007163:Immunosorbent Techniques; D008163:Luminescent Measurements; D008894:Milk Proteins / Q000276:immunology*; D008925:Mites / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test; D015203:Reproducibility of Results; D012130:Respiratory Hypersensitivity / Q000209:etiology / Q000276:immunology; D012680:Sensitivity and Specificity
D000485:Allergens; D004391:Dust; D008894:Milk Proteins; D007073:Immunoglobulin E
Evaluation de la fiabilité de la technique MAST pour les pneumallergènes et les trophallergènes. Etude biologique.