title: Identification of the replication region of the Lactobacillus acidophilus plasmid pLA106.
abstract: The complete nucleotide sequence of plasmid pLA106 (2862 bp) from Lactobacillus acidophilus TK8912 was determined. Based on this sequence, the location of the genes for mobilization-plasmid recombination protein (mob), replication origin (ori), transcriptional repressor protein (repA) and replication initiation protein (repB) were predicted. Deletion analysis showed that the 1.4-kb PstI-EcoRV fragment carrying the ori, repA and repB genes is able to replicate in Lactobacillus and Escherichia coli cells. The plasmid pLA106 appears to have most features of the pLS1/pE194 plasmid family.
authors.affiliation: School of Pharmacy, Kobe-Gakuin University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sano
authors.forename: M
authors.initials: M
authors.lastname: Otani
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: R
authors.initials: R
authors.lastname: Kawamura
authors.forename: M
authors.initials: M
authors.lastname: Umesaki
authors.forename: Y
authors.initials: Y
authors.lastname: Ohi
authors.forename: C
authors.initials: C
authors.lastname: Umezawa
authors.forename: K
authors.initials: K
authors.lastname: Kanatani
mesh_terms: D000595:Amino Acid Sequence; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D007779:Lactobacillus acidophilus / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids