[The identification of the genetic subtypes of HIV-1 circulating on the territory of Russia].
In this work the results of the genotyping of HIV strains obtained from 12 patients residing in Russia are presented. Proviral DNA from lysates of mononuclear cells was amplified with the use of primers, complementary to the conservative site of gene gag p7/p9 with the subsequent sequencing of the amplified material and phylogenetic analysis. 9 strains were classified with subtype B (most frequently occurring in Western countries), 2 of them being found closely related (probably, of African origin). In 2 patients infected from the same source the virus of unusual subtype F/1 was identified. This is the second case of the detection of this subtype in the world. One strain belonged to subtype G. in spite of the fact that in accordance with the epidemiological history only 3 patients got infection outside Russia, molecular epidemiological studies showed the genetic heterogeneity of the circulating strains and suggested the diversity of sources of the penetration of HIV infection to Russia.
Leinikki
P
P
National Public Health Institute, Helsinki, Finland.
Liitsola
K
K
Laukkanen
T
T
Salminen
M
M
Smol'skaia
T
T
Korovina
G
G
Vlasov
N
N
D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008214:Lymphocytes / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012426:Russia
D017931:DNA Primers; D004279:DNA, Viral
Identifikatsiia geneticheskikh subtipov VICh-1, tsirkuliruiushchikh na territorii Rossii.