Interaction of flagellar inner arm dynein isolated from sea urchin sperm with microtubules in the presence of ATP.
We have isolated C/A dynein, which is considered to be a component of inner arms, from flagellar axonemes of sea urchin sperm (E. Yokota, I. Mabuchi, J. Cell Sci. 107, 345-351 (1994). C/A dynein binds to and bundles the microtubules in the absence of ATP. In contrast to outer arm 21S dynein, C/A dynein is not released from the microtubules in the presence of ATP (E. Yokota, I. Mabuchi, J. Cell Sci. 107, 353-361 (1994)). We further investigated the interaction of C/A dynein with microtubules in the presence of ATP. The turbidity at 350 nm of a mixture of C/A dynein and microtubules increased by the addition of ATP. Both the initial rate and final extent of the turbidity increase were dependent on C/A dynein or ATP concentration and were inhibited by vanadate. ATP hydrolysis by C/A dynein was linear during the time course of the turbidity increase. Negative staining electron microscopy revealed that microtubular bundles which formed in the presence of C/A dynein became thicker and longer after addition of ATP. Furthermore, sliding movements of microtubule(s) in the individual bundles were observed in the presence of ATP. This mode of interaction of C/A dynein with microtubules was distinct from that of flagellar or ciliary dyneins reported so far. These results suggest that C/A dynein, as a component of inner arms, may play a distinct role in the flagellar movement of sea urchin sperm.
Yokota
E
E
Department of Biology, College of Arts and Sciences, University of Tokyo, Japan.
Mabuchi
I
I
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement; D004398:Dyneins / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D008854:Microscopy, Electron; D008870:Microtubules / Q000201:enzymology*; D009391:Nephelometry and Turbidimetry; D012617:Sea Urchins; D013082:Sperm Tail / Q000201:enzymology* / Q000648:ultrastructure; D014638:Vanadates / Q000494:pharmacology
D014638:Vanadates; D000255:Adenosine Triphosphate; D004398:Dyneins