Protein kinase C in focal ischemic rat brain: dual autoradiographic analysis of [14C]iodoantipyrine (IAP) and [3H]phorbol-12,13-dibutyrate (PDBu).
Protein kinase C (PKC) activity was measured in rat brain with 2 h of middle cerebral artery (MCA) and common carotid artery (CCA) occlusion, using dual autoradiography of [14C]iodoantipyrine (IAP) and [3H]phorbol-12,13-dibutyrate (PDBu). In the ischemic brain, it required more than 120 min of incubation to obtain a plateau in PDBu binding. In contrast, the binding of PDBu in non-ischemic brain reached a plateau with incubation for 60 min. This delay of PDBu binding in the ischemic brain suggests that the affinity of this ligand is reduced due to a change in structure of the cell membrane caused by ischemia. PDBu binding in the ischemic brain increased significantly compared to the non-ischemic brain. This finding provides further evidence that excessive activation of PKC in the ischemic brain may play an important role in ischemic neuronal damage.
Tohyama
Y
Y
Department of Neurosurgery, Asahikawa Medical College, Hokkaido, Japan.
Sako
K
K
Yonemasu
Y
Y
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D001345:Autoradiography; D001921:Brain / Q000098:blood supply* / Q000201:enzymology*; D002250:Carbon Radioisotopes; D017536:Carotid Artery, Common; D002536:Cerebral Arteries; D002560:Cerebrovascular Circulation; D002546:Ischemic Attack, Transient / Q000201:enzymology*; D008297:Male; D015240:Phorbol 12,13-Dibutyrate / Q000493:pharmacokinetics*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D000983:Antipyrine; C008135:iodoantipyrine