title: Molecular cloning of cDNA encoding two types of pituitary gonadotropin alpha subunit from the goldfish, Carassius auratus.
abstract: Two types of cDNA encoding the alpha subunit of pituitary gonadotropin (GTH) were cloned by polymerase chain reaction (PCR) for goldfish pituitary cDNA library. The goldfish GTH-alpha subunit cDNAs, designated as alpha 1 and alpha 2, encode 117 and 118 amino acids, respectively, including a 23-amino-acid signal peptide. These two types of cDNAs showed high homology in nucleotide and amino acid sequence, but deletion of a triplet nucleotides was present in alpha 1. Deduced amino acid sequences represented differences in four residues between alpha 1 and alpha 2 subunits. Analyses of goldfish genomic DNA revealed that each individual goldfish possesses two distinct genes relevant to GTH-alpha 1 and -alpha 2 subunits. Results of reverse transcriptase-PCR analysis suggest that these two GTH-alpha subunit genes are expressed at different levels without relation to sex and gonadal maturity.
authors.affiliation: Department of Aquatic Bioscience, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshiura
authors.forename: K
authors.initials: K
authors.lastname: Aida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D005260:Female; D015292:Glycoprotein Hormones, alpha Subunit / Q000737:chemistry / Q000235:genetics*; D006054:Goldfish / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D017385:Sequence Homology
chemical_list: D018076:DNA, Complementary; D015292:Glycoprotein Hormones, alpha Subunit; D012194:RNA-Directed DNA Polymerase