title: A cytoplasmic actin gene from the silkworm Bombyx mori is expressed in tissues of endodermal origin and previtellogenic germ cells of transgenic Drosophila.
abstract: A cytoplasmic actin gene from Bombyx mori introduced into Drosophila melanogaster by P-element mediated transformation, is efficiently transcribed in larvae, pupae and adults of the host. The exogenous mRNA has the same size as the one observed in the Bombyx cells and the intron located within the coding region is properly excised, indicating a correct recognition of the exogenous sequences by the Drosophila transcriptional and splicing machineries. The expression of the Bombyx gene in Drosophila tissues was determined by transforming flies with a hybrid gene in which a large part of the Bombyx actin coding sequences was replaced by those of the bacterial lac Z gene. This chimaeric gene is specifically and highly expressed, from the embryo to the adult of the transgenic lines, in tissues of endodermal origin, the midgut and its derivatives, i.e. gastric caeca, the outer layer of the proventriculus, and in the Malpighian tubules. This gene is also expressed, at a lower level, in germ cells but restricted to the sixteen cell cysts during previtellogenesis. The expression of the Bombyx gene during development of transgenic flies was compared to that of the two Drosophila endogenous cytoplasmic actin genes and the results are discussed.
authors.affiliation: Université Lyon 1, Centre de Génétique Moléculaire et Cellulaire, Villeurbanne, France.
authors.forename: B
authors.initials: B
authors.lastname: Horard
authors.forename: B
authors.initials: B
authors.lastname: Bello
authors.forename: E G
authors.initials: EG
authors.lastname: Abraham
authors.forename: M
authors.initials: M
authors.lastname: Coulon-Bublex
authors.forename: A
authors.initials: A
authors.lastname: Garel
authors.forename: N
authors.initials: N
authors.lastname: Mounier
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D012831:Bombyx / Q000235:genetics*; D003593:Cytoplasm; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004707:Endoderm; D015870:Gene Expression; D017344:Genes, Insect; D005854:Germ Cells / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D014818:Vitellogenesis
chemical_list: D000199:Actins; D012313:RNA