[Isolation, culture and characterization of astrocytes from adult monkey brains].
We have developed protocols for the isolation, the culture and the immunocytochemical characterization of astrocytes from simian adult brains. We have obtained pure astrocyte cultures without contamination with other cells present in brain. No microglial cells, oligodendrocytes, neurons or fibroblasts were detected by specific staining in immunocytochemistry.
Guillemin
G
G
Service de Neurovirologie, CEA, DSV/DRMSSA/IPSC, Fontenay-aux-Roses, France.
Boussin
F
F
Dormont
D
D
D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology; D000818:Animals; D001253:Astrocytes / Q000473:pathology*; D001921:Brain / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D015498:HIV-2; D007150:Immunohistochemistry; D008253:Macaca mulatta
Isolement, culture et caractérisation d'astrocytes issus de cerveaux de singes adultes.