title: Structural analysis of the oligosaccharide-alditols released by reductive beta-elimination from oviducal mucins of Rana temporaria.
abstract: The carbohydrate chains of the mucins which constitute the jelly coat surrounding the eggs of Rana temporaria were released by alkaline borohydride treatment. Neutral and acidic oligosaccharide-alditols were purified by ion-exchange chromatography and HPLC. From the structural analysis, based upon 1H and 13C-NMR spectroscopy in combination with MALDI-TOF, the following glycan units are proposed.
authors.affiliation: Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille (Flanders-Artois), Villeneuve d'Ascq, France.
authors.forename: E
authors.initials: E
authors.lastname: Maes
authors.forename: D
authors.initials: D
authors.lastname: Florea
authors.forename: F
authors.initials: F
authors.lastname: Delplace
authors.forename: J
authors.initials: J
authors.lastname: Lemoine
authors.forename: Y
authors.initials: Y
authors.lastname: Plancke
authors.forename: G
authors.initials: G
authors.lastname: Strecker
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry*; D010057:Oviducts / Q000378:metabolism*; D011896:Rana temporaria / Q000502:physiology*; D013402:Sugar Alcohols / Q000737:chemistry*
chemical_list: D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols