[Relationship between the UV-induction of exact exclusion of transposons and the function of umuDC, lexA, recA genes and plasmid pkM101].
A pair of isogenic strains-E. coli K-12 and E. coli B/r differing by the status of umuDC genes and presence of pKM101 plasmid-were constructed and the relationship between UV induction of transposons Tn5 and Tn 10 and the gene umuDC function shown. This relationship is not absolute, in contrast to that of point mutations. Induction of precise excision of these transposons can be inhibited by pKM101 plasmid. Induction of precise excision of Tn5 and Tn 10 from the sites under study is absolutely lexA- and recA- dependent.
Rusina
O Iu
OIu
Andreeva
I V
IV
Tiganova
I G
IG
Mirskaia
E E
EE
Skavronskaia
A G
AG
D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D005798:Genes, Bacterial / Q000528:radiation effects*; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000528:radiation effects*; D017354:Point Mutation; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements
Zavisimost' UF-induktsii tochnogo iskliucheniia transpozonov ot funktsii genov umuDC, lexA, recA i plazmidy pKM101.