Induced tolerance and chimaerism in human fetuses using coelocentesis: a medical opportunity to avert genetic disease?
Coelocentesis offers a new opportunity for gaining access to the coelomic cavity of human embryos from 28 days post-fertilization (42 days menstrual age). With this technique, cells can be extracted from the cavity for the genetic typing of embryos in early pregnancy. Coelocentesis may also offer a unique opportunity of inducing tolerance to foreign grafts and chimaerism in these human embryos by replacing donor cells into the coelomic cavity. This cavity appears to be closely associated with the fetal haemopoietic system. The optimal age to inject stem cells designed to produce chimaerism may be at 5-6 weeks embryonic age, and these grafted cells may induce tolerance later in gestation. Two successive coelocenteses would be needed, the first to extract fetal cells to type the fetus, and a second within a few days to inject the donor cells into the coelomic cavity. Alternatively, non-invasive methods of diagnosis such as lower uterine pole extramembranous sampling of fetal trophoblast, or the extraction of fetal cells from maternal blood, could be combined with coelocentesis. If tolerance and chimaerism can be established, repeated tissue grafts could be carried out during fetal life and after birth, so that disorders caused by single or multiple gene defects in the haemopoietic system and other organs may be corrected.
Edwards
R G
RG
Churchill College, Cambridge, UK.
Jauniaux
E
E
Binns
R M
RM
Layton
M
M
Jurkovic
D
D
Grillo
T A
TA
Campbell
S
S
D002678:Chimera; D002823:Chorion; D005260:Female; D016332:Fetal Tissue Transplantation; D030342:Genetic Diseases, Inborn / Q000628:therapy*; D005865:Gestational Age; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007108:Immune Tolerance; D019152:Paracentesis; D011247:Pregnancy