Capillary electrophoresis of supercoiled and linear DNA in dilute hydroxyethyl cellulose solution.
Capillary electrophoresis in dilute hydroxyethyl cellulose is shown to separate supercoiled DNA in the size range 2000-16,000 base pairs. The plasmids migrate more slowly than linear ds-DNA of the same sizes. Plasmid bandwidths are larger than observed for ds-DNA, allowing identification of the type of DNA by bandwidth. The differing dependence of mobility on chain length can be explained by assuming that a plasmid migrates as an elastic rod, while ds-DNA migrates as a wormlike chain.
Hammond
R W
RW
Department of Chemistry, School of Medicine, University of Michigan, Ann Arbor 48109, USA.
Oana
H
H
Schwinefus
J J
JJ
Bonadio
J
J
Levy
R J
RJ
Morris
M D
MD
D002482:Cellulose / Q000031:analogs & derivatives; D004247:DNA / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*
D004278:DNA, Superhelical; D002482:Cellulose; C002283:hydroxyethylcellulose; D004247:DNA