title: A tandem array of 5S ribosomal RNA genes in Pythium irregulare.
abstract: The 5S ribosomal RNA genes of the oomycete Pythium irregulare exist in tandem arrays unlinked to the rDNA repeat unit. A clone with a 9.2-kb insert containing an array of 5S genes was identified in a lambda genomic library and was characterized by restriction mapping and partial sequencing. The array consisted of 9 apparently identical 5S genes and their spacers in tandem, followed by a diverged 5S-like sequence that is likely to be a pseudogene. This gene arrangement, although almost universal in plants and animals, is rare in fungi and protists.
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Belkhiri
authors.forename: H
authors.initials: H
authors.lastname: Intengan
authors.forename: G R
authors.initials: GR
authors.lastname: Klassen
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics; D005800:Genes, Fungal; D015698:Genomic Library; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D011775:Pythium / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012341:RNA, Ribosomal, 5S