title: Fluoro-Jade: a novel fluorochrome for the sensitive and reliable histochemical localization of neuronal degeneration.
abstract: Fluoro-Jade is an anionic fluorochrome capable of selectively staining degenerating neurons in brain slices. The histochemical application of Fluoro-Jade results in a simple, sensitive and reliable method for staining degenerating neurons and their processes. The technique will detect neuronal degeneration resulting from exposure to a variety of neurotoxic insults. Fluoro-Jade can be combined with other fluorescent methodologies including immunofluorescence, fluorescent axonal tract tracing, and fluorescent Nissl counterstaining. Compared to conventional methodologies, Fluoro-Jade is a more sensitive and definitive marker of neuronal degeneration than hematoxylin and eosin (H&E) or Nissl type stains, while being comparably sensitive yet considerably simpler and more reliable than suppressed silver techniques.
authors.affiliation: Division of Neurotoxicology, Food and Drug Administration, Jefferson, AR 72079-9502, USA.
authors.forename: L C
authors.initials: LC
authors.lastname: Schmued
authors.forename: C
authors.initials: C
authors.lastname: Albertson
authors.forename: W
authors.initials: W
authors.lastname: Slikker
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D000959:Antihypertensive Agents; D001369:Axons / Q000737:chemistry; D016291:Dizocilpine Maleate; D015259:Dopamine Agents; D004801:Eosine Yellowish-(YS); D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D015353:Eye Enucleation; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005456:Fluorescent Dyes; D006213:Hallucinogens; D006416:Hematoxylin; D007050:Ibogaine; D007608:Kainic Acid; D008297:Male; D008670:Metals; D009410:Nerve Degeneration / Q000502:physiology*; D009562:Nissl Bodies / Q000737:chemistry; D009574:Nitro Compounds; D010622:Phencyclidine; D011422:Propionates; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D016622:Silver Staining; D013238:Stereotaxic Techniques
chemical_list: D000959:Antihypertensive Agents; D015259:Dopamine Agents; D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D005456:Fluorescent Dyes; D006213:Hallucinogens; D008670:Metals; D009574:Nitro Compounds; D011422:Propionates; D007050:Ibogaine; D016291:Dizocilpine Maleate; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D010622:Phencyclidine; C015392:3-nitropropionic acid; D007608:Kainic Acid; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin