Starch utilization by Bacteroides ovatus isolated from the human large intestine.
Starch supported growth of continuous cultures of Bacteroides ovatus when this carbohydrate provided the sole source of carbon and energy. Inducible amylase and alpha-glucosidase activities were inversely related to dilution rate in starch-limited and starch-excess chemostats over the dilution rate (D) range D = 0.03/h to D =0.20/h, and were partly repressed during growth under conditions of starch-excess. Preparative isoelectric focusing of B. ovatus cytoplasmic extracts indicated the existence of three distinct starch-hydrolyzing enzymes. Incubation of active fractions from the isoelectric focusing cell with maltose and a variety of low-molecular-weight oligosaccharides (maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose) identified a single amylase activity, an enzyme with combined beta-amylase and glucoamylase/alpha-glucosidase properties, and also a possible pullulanase. The ability of B. ovatus to synthesize several starch-hydrolyzing enzymes with different specificities and activities may confer a significant competitive advantage to this organism in the colonic ecosystem.
Degnan
B A
BA
Medical Research Council, Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK.
Macfarlane
S
S
Quigley
M E
ME
Macfarlane
G T
GT
D001439:Bacteroides / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000302:isolation & purification / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007420:Intestine, Large / Q000382:microbiology*; D007525:Isoelectric Focusing; D009844:Oligosaccharides / Q000378:metabolism; D013213:Starch / Q000378:metabolism*; D013379:Substrate Specificity; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000378:metabolism; D001614:beta-Amylase / Q000302:isolation & purification / Q000378:metabolism
D009844:Oligosaccharides; D013213:Starch; D006026:Glycoside Hydrolases; D001614:beta-Amylase; D000520:alpha-Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase; C100181:pullulanase