The influence of FSH, LH and testosterone on steroidsecretion by two subpopulations of porcine granulosa cells.
The first objective of this research was to define the capacity of two types granulosa cells (mural MGs and antral AGc) to secrete estradiol (E2) in the presence of an androgen substrate (testosterone). The second objective was to identify the synergism between gonadotropic hormones such as LH and FSH and testosterone (T) as measured by E2 secretion by MGc and AGc. MGc were separated from AGc by filtration through a 32.5 microns nylon filter. In the first experiment, control cultures were grown in M199 medium supplemented with 10% calf serum while the other cultures were supplemented with either T alone at a concentration of 1 x 10(-7) M or with 1.0 x 10(-3) M, 0.5 x 10(-3) M, or 0.1 x 10(-3) M aromatase inhibitor CGS 16949A plus T. In the second experiment, cultures were enriched either with 1 x 10(-7) M T or with 100 ng LH plus T or 100 ng FSH plus T. After 24 hours of cultivation all cultures were terminated and the media were frozen at -20 degree C for further steroid analysis. Basal E2 secretion was always higher in MGc than in AGc. Adding testosterone to the culture medium decreased E2 secretion by MGc but significantly increased E2 secretion by AGc. LH in the absence of T was without effect on E2 secretion by both Gc types. FSH alone stimulated E2 secretion by MGc and decreased E2 secretion by AGc. Synergistic action of FSH and LH with T on E2 secretion by both types of granulosa cells was observed. These findings provide the basis for further analysis of the differences in aromatizing ability of the two subpopulations of Gc during follicular development.
Duda
M
M
Department of Physiology, Jagiellonian University, Cracow, Poland.
D000818:Animals; D047072:Aromatase Inhibitors; D003470:Culture Media; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology*; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*; D013552:Swine; D013739:Testosterone / Q000494:pharmacology*
D047072:Aromatase Inhibitors; D003470:Culture Media; D013256:Steroids; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone