title: Large-scale mammalian cell culture.
abstract: Mammalian cell culture continues to draw major research efforts. A great deal of progress has recently been made in cellular physiology, especially in factors adversely affecting cell growth or viability. Through molecular genetic manipulation, cells are more readily cultivated in a medium free of animal proteins. Achieving a high cell concentration and high viability continue to be common themes in engineering research. The need to implement a control policy for fed-batch and perfusion cultures has prompted increased efforts in process monitoring and control. Integrating these advances will be beneficial for ensuring product quality and process consistency.
authors.affiliation: Chemical Engineering and Materials Science Department, University of Minnesota, 421 Washington Avenue South East, Minneapolis, MN 55455, USA.
authors.forename: W S
authors.initials: WS
authors.lastname: Hu
authors.forename: J G
authors.initials: JG
authors.lastname: Aunins
mesh_terms: D000818:Animals; D017209:Apoptosis; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D016923:Cell Death; D002460:Cell Line; D003470:Culture Media
chemical_list: D003470:Culture Media