title: Determination of okadaic acid by micellar electrokinetic chromatography with ultraviolet detection.
abstract: Micellar electrokinetic chromatography (MEKC) with ultraviolet (UV) detection was applied for the determination of non-derivatized phycotoxins associated with diarrhoetic shellfish poisoning. A detection limit for 40 pg of okadaic acid (OA) was achieved. The UV intensities of this toxin measured at 200 nm showed good linearity in the range 40-640 pg. OA was detected in mussels spiked with 10 ng/g whole tissue. The presence of OA and dinophysistoxin-2 was observed in the crude extract of the dinoflagellate Prorocentrum lima.
authors.affiliation: CEMATMA, Laboratoire de Chimie Analytique, ESPCI, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Bouaïcha
authors.forename: M C
authors.initials: MC
authors.lastname: Hennion
authors.forename: P
authors.initials: P
authors.lastname: Sandra
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002845:Chromatography / Q000379:methods*; D004586:Electrophoresis; D008387:Marine Toxins / Q000737:chemistry; D019319:Okadaic Acid / Q000302:isolation & purification*; D010839:Phytoplankton / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D008387:Marine Toxins; D019319:Okadaic Acid