title: Involvement of PA/plasmin system in the processing of pro-MMP-9 and in the second step of pro-MMP-2 activation.
abstract: Pro-MMP2 activation is a two-step process resulting in (1) an intermediate 64 kDa form generated by the MT1-MMP activity, and (2) a mature 62 kDa form. Addition of plasminogen to HT1080 cells cultured under various conditions, or to their membrane preparation, induced a complete conversion of the intermediate MMP-2 form to the mature one, and processing of pro-MMP-9. The pro-MMP-2 activation was inhibited by plasmin inhibitors and anti-uPA antibody. These results provide evidence for involvement of the PA/plasmin system in the second step of MMP-2 activation.
authors.affiliation: Laboratory of Biology, University of Liege, Belgium.
authors.forename: E N
authors.initials: EN
authors.lastname: Baramova
authors.forename: K
authors.initials: K
authors.lastname: Bajou
authors.forename: A
authors.initials: A
authors.lastname: Remacle
authors.forename: C
authors.initials: C
authors.lastname: L'Hoir
authors.forename: H W
authors.initials: HW
authors.lastname: Krell
authors.forename: U H
authors.initials: UH
authors.lastname: Weidle
authors.forename: A
authors.initials: A
authors.lastname: Noel
authors.forename: J M
authors.initials: JM
authors.lastname: Foidart
mesh_terms: D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism*; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism*; D018093:Gelatinases / Q000378:metabolism*; D006801:Humans; D008566:Membranes / Q000378:metabolism; D008666:Metalloendopeptidases / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D014407:Tumor Cells, Cultured
chemical_list: D004792:Enzyme Precursors; D010960:Plasminogen Activators; D005341:Fibrinolysin; D018093:Gelatinases; D008666:Metalloendopeptidases; C069860:progelatinase; D003012:Microbial Collagenase