title: Malignant astrocytoma-derived region of common amplification in chromosomal band 17p12 is frequently amplified in high-grade osteosarcomas.
abstract: Recently, we reported a new amplification event that involves marker D17S67 in 17p12 in three malignant astrocytomas of patients with a very short survival. The amplified region may contain an oncogene implicated in astrocytoma tumorigenesis. To determine the extent of the amplified regions, we constructed a yeast artificial chromosome contig spanning the D17S67 region and tested the amplification status of markers that map to the contig. We determined a commonly amplified region between markers D17S1311 and D17S1875 with a maximal length of 1,630 kb. By using marker 745R, from within the commonly amplified region, we screened 60 high-grade astrocytomas but could not detect additional tumors with the amplification event. This suggests that the incidence of the amplification event in high-grade astrocytoma is low (5%). It has recently been shown by comparative genomic hybridization that amplification of 17p11-p12 is a frequent event in high-grade osteosarcomas, occurring in 20-30% of cases. Since the commonly amplified region is within 17p12, we tested 745R in 20 osteosarcomas, including 6 lung metastases, and detected amplification in 9 cases (45%). Marker 745R was found to be amplified in 4 of the 6 lung metastases (66%). From this frequent involvement and the association with clinically aggressive astrocytomas we conclude that for both tumor types presence of the amplification event seems to correlate with aggressive clinical behaviour.
authors.affiliation: Department of Human Genetics, Academic Medical Center, University of Amsterdam, The Netherlands.
authors.forename: T J
authors.initials: TJ
authors.lastname: Hulsebos
authors.forename: E H
authors.initials: EH
authors.lastname: Bijleveld
authors.forename: N T
authors.initials: NT
authors.lastname: Oskam
authors.forename: A
authors.initials: A
authors.lastname: Westerveld
authors.forename: S
authors.initials: S
authors.lastname: Leenstra
authors.forename: P C
authors.initials: PC
authors.lastname: Hogendoorn
authors.forename: J
authors.initials: J
authors.lastname: Bras
mesh_terms: D001254:Astrocytoma / Q000235:genetics*; D015139:Blotting, Southern; D002869:Chromosome Aberrations; D018244:Chromosomes, Artificial, Yeast; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D016158:Genes, p53; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics* / Q000556:secondary; D016133:Polymerase Chain Reaction
chemical_list: D004273:DNA, Neoplasm; D005819:Genetic Markers