title: Matrix-assisted laser desorption/ionisation mass spectrometry of transfer ribonucleic acids isolated from yeast.
abstract: tRNATyr and tRNASer purified from bulk brewer's yeast tRNA were subjected to analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Choosing a mixture of 2,4,6- and 2,3,4-trihydroxy-acetophenone and diammonium citrate as matrix a mass resolution of up to 220 (FWHM) was achieved in the linear mode of operation. Cation adduct suppression by addition of cation exchange beads and a chelating agent (CDTA) is shown to substantially improve mass resolution for this class of molecules.
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia.
authors.forename: I
authors.initials: I
authors.lastname: Gruic-Sovulj
authors.forename: H C
authors.initials: HC
authors.lastname: Lüdemann
authors.forename: F
authors.initials: F
authors.lastname: Hillenkamp
authors.forename: I
authors.initials: I
authors.lastname: Weygand-Durasevic
authors.forename: Z
authors.initials: Z
authors.lastname: Kucan
authors.forename: J
authors.initials: J
authors.lastname: Peter-Katalinic
mesh_terms: D012331:RNA, Fungal / Q000737:chemistry*; D012343:RNA, Transfer / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D012331:RNA, Fungal; D012343:RNA, Transfer