title: Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos.
abstract: Establishment of the vertebrate body plan requires a variety of signaling molecules. In a search for tyrosine kinases expressed in early zebrafish embryos, a model system for the study of vertebrate development, we discovered Jak1 kinase to be maternally encoded and the mRNA evenly distributed among the cells of blastula-stage embryos. Injection of RNA-encoding dominant-negative Jak1 kinases reduces a specific cell migration, epiboly, and results in the reduction of goosecoid expression and of anterior structures. This work establishes that, in addition to its role in signal transduction of cytokines in adult tissues, Jak1 kinase has a role in early vertebrate development.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02114, USA. conway@nucleus.harvard.edu
authors.forename: G
authors.initials: G
authors.lastname: Conway
authors.forename: A
authors.initials: A
authors.lastname: Margoliath
authors.forename: S
authors.initials: S
authors.lastname: Wong-Madden
authors.forename: R J
authors.initials: RJ
authors.lastname: Roberts
authors.forename: W
authors.initials: W
authors.lastname: Gilbert
mesh_terms: D000818:Animals; D002465:Cell Movement; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D053613:Janus Kinase 1; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins; D014157:Transcription Factors; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins
chemical_list: D004268:DNA-Binding Proteins; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C497995:gsc protein, zebrafish; D011505:Protein-Tyrosine Kinases; D053613:Janus Kinase 1; C507907:jak1 protein, zebrafish