title: Replication of plasmids with the p15A origin in Shewanella putrefaciens MR-1.
abstract: The plasmid pACYC184 was introduced into Shewanella putrefaciens MR-1 by electroporation. In 100% of the transformants examined, the plasmid was maintained as a free replicon outside the chromosome. This was the case whether or not the plasmid contained a 224-bp DNA insert derived from an open-reading frame of MR-1 genomic DNA. Therefore, in contrast to a report in the literature, plasmids containing the p15A origin of replication can replicate freely in S. putrefaciens MR-1, and do not make convenient vectors for gene replacement in this bacterium. However, we found that plasmids with the pMB1 origin of replication (e.g. pBR322) cannot replicate in MR-1 and could therefore have potential as vectors for gene replacement.
authors.affiliation: Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Myers
authors.forename: J M
authors.initials: JM
authors.lastname: Myers
mesh_terms: D015139:Blotting, Southern; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000235:genetics*; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin; D014170:Transformation, Genetic
chemical_list: D004251:DNA Transposable Elements