title: Inducing cholesterol precipitation from pig bile with beta-cyclodextrin and cholesterol dietary supplementation.
abstract: METHODS
  In this study, pigs fed for 3 weeks a well-balanced semi-purified diet enriched with 0.3% cholesterol and 0, 5 or 10% beta-cyclodextrin were proposed as new animal donors of gallbladder bile exhibiting different rates of cholesterol crystallization, in order to gain insight into the early mechanisms underlying cholesterol precipitation in vivo. The appearance and growth of cholesterol crystals were monitored in the incubated freshly collected gallbladder biles through light microscopy and concomitant time-sequential determination of crystallized cholesterol concentration, and interpreted in terms of the composition of the bile.
  
  
  RESULTS
  Although the concentration of total lipids and proteins and the relative proportions of bile acids, phospholipids, and cholesterol remained unchanged under beta-cyclodextrin, the cholesterol crystallization increased in the following order: 0<<10<5% beta-cyclodextrin. Concomitantly, the proportion of chenodeoxycholic acid in bile, and the hydrophobicity index of the biliary bile acid mixture increased in the following order: 0<5<10% beta-cyclodextrin (the same as reported elsewhere for the decrease in the antinucleating ApoA1), while sn-2 arachidonoyl biliary lecithins were specifically increased with 5% beta-cyclodextrin in the diet.
  
  
  CONCLUSIONS
  We hypothesized that lecithin molecular species may be the determinant factor in modulating high cholesterol crystallization rates in biles otherwise enriched with hydrophobic bile acids.
authors.affiliation: Laboratoire d'Ecologie et de Physiologie du Système Digestif, INRA, Jouy-en-Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Juste
authors.forename: I
authors.initials: I
authors.lastname: Catala
authors.forename: M
authors.initials: M
authors.lastname: Riottot
authors.forename: M
authors.initials: M
authors.lastname: André
authors.forename: M
authors.initials: M
authors.lastname: Parquet
authors.forename: B
authors.initials: B
authors.lastname: Lyan
authors.forename: F
authors.initials: F
authors.lastname: Béguet
authors.forename: J
authors.initials: J
authors.lastname: Ferézou-Viala
authors.forename: C
authors.initials: C
authors.lastname: Sérougne
authors.forename: N
authors.initials: N
authors.lastname: Domingo
authors.forename: C
authors.initials: C
authors.lastname: Lutton
authors.forename: H
authors.initials: H
authors.lastname: Lafont
authors.forename: T
authors.initials: T
authors.lastname: Corring
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry* / Q000187:drug effects; D001647:Bile Acids and Salts / Q000032:analysis; D011232:Chemical Precipitation; D002784:Cholesterol / Q000737:chemistry*; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D003460:Crystallization; D003505:Cyclodextrins / Q000008:administration & dosage* / Q000032:analysis; D005243:Feces / Q000737:chemistry; D005500:Follow-Up Studies; D005503:Food Additives / Q000008:administration & dosage*; D008055:Lipids / Q000032:analysis; D008297:Male; D010713:Phosphatidylcholines / Q000032:analysis; D013552:Swine; D047392:beta-Cyclodextrins
chemical_list: D001647:Bile Acids and Salts; D002791:Cholesterol, Dietary; D003505:Cyclodextrins; D005503:Food Additives; D008055:Lipids; D010713:Phosphatidylcholines; D047392:beta-Cyclodextrins; D002784:Cholesterol; C031215:betadex