T cell receptor V beta gene usage in Guillain-Barré syndrome.
We set out to determine whether the T cell receptor (TCR) V beta gene usage in acute inflammatory demyelinating polyradiculoneuropathy (AIDP) is restricted. We separated activated from non-activated peripheral blood T cells with anti-IL2 receptor (anti-CD25) antibody-labelled magnetic beads from four AIDP patients and four normal control (NC) subjects. The TCR V beta gene usage of circulating activated and non-activated T cells was heterogeneous in all the patients and controls, but the activated T cells of all four of the AIDP patients showed a more limited usage of V beta genes and enhanced V beta 15 usage, as compared to the non-activated T cells. This was not seen in the healthy controls. The activated and non-activated T cells from a patient with acute motor and sensory axonal neuropathy (AMSAN) showed a similar V beta gene usage to that of the controls. From a further patient with AIDP, we studied the V beta gene usage of short-term T cell lines reactive to the peripheral nerve myelin proteins P2, P0 and the P0 peptide amino acid sequence 194-208. The V beta gene usage of the lines was heterogeneous, with enhanced usage of V beta 15 in the cell line responsive to the Pzero peptide. We conclude that T cells activated during the immune response associated with AIDP preferentially used V beta 15, which may indicate a restricted response to a common antigen, or a role for an as yet undefined superantigen in the pathogenesis of AIDP.
Khalili-Shirazi
A
A
Department of Neurology, UMDS, Guy's Campus, London, UK.
Gregson
N A
NA
Hall
M A
MA
Hughes
R A
RA
Lanchbury
J S
JS
D000328:Adult; D000368:Aged; D002460:Cell Line; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009185:Myelin Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011129:Polyradiculoneuropathy / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D015375:Receptors, Interleukin-2 / Q000276:immunology; D013601:T-Lymphocytes / Q000378:metabolism
D018076:DNA, Complementary; D009185:Myelin Proteins; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2; D012313:RNA