Generation of superoxide anion and induction of superoxide dismutase and peroxidase in bean leaves infected with pathogenic fungi.
Generation of superoxide anion (O2-.) and peroxidase activity were significantly increased in bean leaves infected with incompatible and compatible pathogens: Botrytis fabae and Botrytis cinerea, respectively, but the induction was greater on direct inoculation with B. fabae, than with B. cinerea. A slightly higher O2-. level was also detected in the parts of leaves surrounding the inoculation side. Overproduction of O2-. was observed earlier than the increase in peroxidase activity. Pretreatment of the leaves with methyl jasmonate enhanced both O2-. production and peroxidase activity following inoculation with B. cinerea. Induction of superoxide dismutase activity after the infection was less pronounced than changes in O2-. level. The differences in the rate of NADH oxidation in the extracts from control and inoculated leaves, correlated with the differences in the rate of O2-. production. The results indicate that O2-. level is one of the essential factors responsible for the difference in the interactions between bean plant and compatible and incompatible pathogens.
Urbanek
H
H
Department of Plant Physiology and Biochemistry, University of Lódź, Poland.
Gajewska
E
E
Karwowska
R
R
Wielanek
M
M
D007887:Fabaceae / Q000201:enzymology* / Q000382:microbiology; D003904:Mitosporic Fungi / Q000302:isolation & purification* / Q000472:pathogenicity; D009195:Peroxidase / Q000096:biosynthesis*; D018515:Plant Leaves / Q000201:enzymology* / Q000382:microbiology; D010946:Plants, Medicinal; D013482:Superoxide Dismutase / Q000096:biosynthesis*; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D009195:Peroxidase; D013482:Superoxide Dismutase