Triton X-100 induces apoptosis in human hepatoma cell lines.
The detergent Triton X-100 was used to establish a model for apoptosis in hepatoma cell lines. The electrophoresis of DNA extracted from 0.01% Triton X-100 treated hepatoma cell lines showed DNA ladder formation, a hallmark of apoptosis. The DNA fragmentation appeared within less than 60 min of the Triton X-100 treatment. Chromatin condensation and apoptotic bodies were observed by hematoxylin and eosin (H & E) stain, and fragmented nucleosome was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) test. Apoptosis was semi-quantitated by measuring the lactate dehydrogenase (LDH) level for cytotoxity. It was found that apoptosis had been induced in more than 90% of the cells treated with Triton X-100 for 150 min. These data show that Triton X-100 efficiently induces the apoptotic cell death in hepatoma cell lines.
Ahn
J M
JM
Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
Kim
S J
SJ
Kim
H
H
Park
C
C
Kim
W H
WH
Park
J H
JH
D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D053938:DNA Fragmentation; D003902:Detergents / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D017830:Octoxynol / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003902:Detergents; D017830:Octoxynol