Baculovirus immediate early gene promoter based expression vectors for transient and stable transformation of insect cells.
A recombinant plasmid vector was constructed in which the bacterial LacZ gene was placed under the control of a Bombyx mori baculovirus early promoter. The vector proved to be active in transfected cultured dipteran and lepidopteran cells. Co-transfection carried out with this recombinant plasmid vector and a plasmid containing the hygromycin phosphotransferase gene followed by selection with the antibiotic hygromycin B, resulted in stable transformation of cultured Drosophila melanogaster Schneider 2 cells. Southern blot analysis of the host cell's genomic DNA in combination with chromosomal in situ hybridization demonstrated that multiple copies of both plasmids were integrated in the host cell's genome.
Vulsteke
V
V
K.U. Leuven Zoological Institute, Belgium.
Janssen
I
I
Vanden Broeck
J
J
De Loof
A
A
Huybrechts
R
R
D000818:Animals; D012831:Bombyx / Q000166:cytology / Q000821:virology; D002460:Cell Line; D004331:Drosophila melanogaster / Q000166:cytology; D015870:Gene Expression; D017781:Genes, Immediate-Early; D005814:Genes, Viral; D005822:Genetic Vectors; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011401:Promoter Regions, Genetic; D018411:Spodoptera / Q000166:cytology; D014162:Transfection; D014170:Transformation, Genetic