title: Site-directed mutagenesis using a PCR-based staggered re-annealing method without restriction enzymes.
authors.affiliation: University of Toronto, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Ailenberg
authors.forename: M
authors.initials: M
authors.lastname: Silverman
mesh_terms: D017931:DNA Primers; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005456:Fluorescent Dyes / Q000378:metabolism; D018093:Gelatinases / Q000235:genetics; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D016650:Fluorescein-5-isothiocyanate