title: Alternate splicing of mouse fusin/CXC chemokine receptor-4: stromal cell-derived factor-1alpha is a ligand for both CXC chemokine receptor-4 isoforms.
abstract: Inspection of the intron splice junction of the mouse chemokine receptor fusin/CXC chemokine receptor R-4 (CXCR-4) revealed a potential in-frame alternative splice site in the region encoding the N-terminal ectodomain of the receptor. Both predicted splice products were detected by reverse transcriptase-PCR. Cell lines of T lymphocyte, B lymphocyte, and macrophage lineage, plus populations of elicited peritoneal exudate cells, thymocytes, and astrocytes coexpressed mRNA for both isoforms. The full length cDNA of the shorter alternate splice product, termed CXCR-4B, was cloned from peritoneal exudate cell RNA. Chinese hamster ovary cells transfected with either isoform, CXCR-4A or CXCR-4B, responded to stromal cell-derived factor-1alpha with a rise in intracellular calcium. Both alternate splice products are therefore functional stromal cell-derived factor-1 alpha receptors.
authors.affiliation: Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: M
authors.initials: M
authors.lastname: Heesen
authors.forename: M A
authors.initials: MA
authors.lastname: Berman
authors.forename: U E
authors.initials: UE
authors.lastname: Höpken
authors.forename: N P
authors.initials: NP
authors.lastname: Gerard
authors.forename: M E
authors.initials: ME
authors.lastname: Dorf
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D054377:Chemokine CXCL12; D018925:Chemokines / Q000378:metabolism*; D019743:Chemokines, CXC; D006224:Cricetinae; D005786:Gene Expression Regulation; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D019718:Receptors, CXCR4; D015728:Receptors, HIV / Q000235:genetics* / Q000378:metabolism
chemical_list: D054377:Chemokine CXCL12; D018925:Chemokines; D019743:Chemokines, CXC; C515256:Cxcl12 protein, mouse; D008024:Ligands; D008565:Membrane Proteins; D019718:Receptors, CXCR4; D015728:Receptors, HIV