Roles of cartilage matrix proteins, chondromodulin-I and -II, in endochondral bone formation: a review.
Insulin induces early chondrogenesis in cultures of a clonal cell-line, ATDC5, which was derived from mouse embryonal carcinoma line AT805. Cartilage-generated matrix components chondromodulin-I (ChM-I) synergistically stimulates growth and differentiation of chondrocytes in the presence or absence of FGF-2. In contrast, ChM-I inhibits the proliferation of vascular endothelial cells and tube formation, thereby further stimulating cartilage growth and inhibiting replacing cartilage by bone in an early stage. Another cartilage-derived chondromodulin-II (ChM-II) also stimulates cartilage growth. However, ChM-II does not inhibit vascularization but stimulates osteoclast differentiation. Therefore, endochondral bone formation is regulated sequentially by cartilage-derived multiple autocrine factors. This opens a new mechanism of regulation of endochondral bone formation.
Suzuki
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Department of Biochemistry, Faculty of Dentistry, Osaka University, Japan. fsuzuki@dent.osaka-u.ac.jp
D000595:Amino Acid Sequence; D000818:Animals; D002356:Cartilage; D002454:Cell Differentiation; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000502:physiology; D006132:Growth Plate / Q000166:cytology* / Q000378:metabolism*; D006133:Growth Substances / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D064235:Matrilin Proteins; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010012:Osteogenesis; D011506:Proteins / Q000502:physiology*
C489408:Cnmd protein, mouse; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C491213:Lect2 protein, mouse; C577540:Matn1 protein, mouse; D064235:Matrilin Proteins; D008565:Membrane Proteins; D011506:Proteins