A Drosophila melanogaster hobo-white(+) vector mediates low frequency gene transfer in D. virilis with full interspecific white(+) complementation.
Transformation of a Drosophila virilis white mutant host strain was attempted using a hobo vector containing the D. melanogaster mini-white(+) cassette (H[w(+), hawN]) and an unmodified or heat shock regulated hobo transposase helper. Two transformant lines were recovered with the unmodified helper (HFL1), one containing only the white(+) marked vector, and a sibling line containing the vector as well as an HFL1 helper integration. An approximate total transformation frequency of 1% is deduced. A high frequency of wing and eye morphology mutants were also observed, suggesting that hobo may have mobilized a related element in D. virilis. The data reaffirms a relatively low transformation vector activity for the hobo transposon in D. virilis; however, nearly full interspecific expression white(+) marker supports its possible function in other species as well.
Gomez
S P
SP
Center for Medical, Agricultural, and Veterinary Entomology, US Department of Agriculture, Gainesville, Florida 32608, USA.
Handler
A M
AM
D018528:ATP-Binding Cassette Transporters; D000818:Animals; D015139:Blotting, Southern; D004254:DNA Nucleotidyltransferases; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005136:Eye Proteins; D018014:Gene Transfer Techniques; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D019476:Insect Proteins / Q000235:genetics*; D016254:Mutagenesis, Insertional; D010641:Phenotype; D014170:Transformation, Genetic; D019895:Transposases
D018528:ATP-Binding Cassette Transporters; D029721:Drosophila Proteins; D005136:Eye Proteins; D019476:Insect Proteins; C059254:w protein, Drosophila; D004254:DNA Nucleotidyltransferases; D019895:Transposases