[The influence of phytohemagglutinin P on CD4+ and CD8+ cells in the course of experimental trichinellosis in mice].
Experiments were carried out with mice of CFW inbred strain infected with 200 Trichinella spiralis larvae and divided into two groups: experimental and control. The experimental mice were injected with phytohaemagglutinin P (PHA-P), 24 h before infection. The all animals were killed between 7-133 days post infection and the pieces of spleens, mesenteric lymph nodes, jejunum and masseter muscles were sectioned in a cryostat and examined with monoclonal antibodies (anti-Thy 1, 2, anti-CD4+ and anti-CD8+) by immunoenzymatic method. In the animals treated with PHA-P, more numerous CD4+ cells were observed in comparison with the control. The CD8+ cells were stimulated only in the muscles. The most important problem were the CD4+ and CD8+ cells inside the larvae capsules. The role of the CD4+ and CD8+ cells in the immunopathological changes in the course of trichinellosis was discussed.
Karmańska
K
K
Instytut Parazytologii im. Witolda Stefańskiego PAN, Wrocław.
Houszka
M
M
Stefaniak
E
E
D000818:Animals; D018791:CD4 Lymphocyte Count / Q000187:drug effects; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects*; D006790:Host-Parasite Interactions; D007583:Jejunum / Q000166:cytology / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D008406:Masseter Muscle / Q000166:cytology / Q000276:immunology; D051379:Mice / Q000469:parasitology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014235:Trichinellosis / Q000276:immunology*
D010835:Phytohemagglutinins; C028784:phytohemagglutinin-P
Wpływ fitohemaglutyniny P na zachowanie sie komórek CD4+ i CD8+ w przebiegu eksperymentalnej włośnicy u myszy.