Leaf senescence in Brassica napus: cloning of senescence related genes by subtractive hybridisation.
A subtractive hybridisation technique was developed to clone cDNAs representing genes that showed enhanced expression during leaf senescence in Brassica napus. A number of different genes were identified that, when analysed by northern hybridisation, showed different patterns of expression during leaf development but were all expressed at increased levels during senescence. Sequence analysis of these cDNAs showed that several types of genes were found including two different proteases, glutamine synthetase, ATP sulphurylase, catalase, metallothionein, ferritin and an antifungal protein. The possible roles of these gene products in the senescence process are discussed.
Buchanan-Wollaston
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Department of Biological Sciences, Wye College, University of London, Ashford, Kent, UK.
Ainsworth
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C
D000595:Amino Acid Sequence; D001937:Brassica / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010940:Plant Proteins