[The prospects for using the genetically engineered surface antigen of the hepatitis B virus (HBsAg) expressed by recombinant poxvirus strains as the basis for vaccines against hepatitis B].
The use of a recombinant poxvirus (RPV) strain, expressing HBsAg in the process of reproduction in different bioreactor systems under stationary and bioreactor conditions of cultivation, made it possible to obtain highly purified HBsAg. The identity and purity of HBsAg was confirmed by the analysis of its amino acid composition, SDS electrophoresis in polyacrylamide gel, electron microscopy and high-performance liquid chromatography. Good prospects of the use of RPV-expressed gene engineering HBsAg as the basis vaccines against hepatitis B was demonstrated in 10 experimental batches of vaccine. All batches of the preparation had pronounced immunogenicity and were safe and nontoxic in animal experiments. The ID50 of experimental batches did not exceed 211 ng/ml, which, according to the data of comparative experiments, was lower than, or equal to, corresponding values of analogous foreign commercial preparations, based on plasma or yeast HBsAg.
Zheleznova
N V
NV
Mukomolov
S L
SL
Zhebrun
A B
AB
Noskov
F S
FS
Malkova
I I
II
Shargorodskaia
E P
EP
Al'tshteĭn
A D
AD
Pliutnikova
V A
VA
Lavrent'eva
I N
IN
Ptichnikova
N N
NN
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D005818:Genetic Engineering / Q000379:methods; D006168:Guinea Pigs; D006509:Hepatitis B / Q000517:prevention & control*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D017325:Hepatitis B Vaccines / Q000032:analysis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D007114:Immunization; D051379:Mice; D011212:Poxviridae / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000032:analysis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification
D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D014614:Vaccines, Synthetic
Perspektivy ispol'zovaniia genno-inzhenernogo poverkhnostnogo antigena virusa gepatitia B (HBsAg), ekspressiruemogo rekombinantnymi shtammami poksvirusov, v kachestve osnovy vaktsin protiv gepatita B.