Functional characterization of a human DNase-like protein encoded by a gene positioned in Xq28.
Xib, a gene recently reported to reside on the q28 region of the human X chromosome [Pergolizzi et al. (1996) Gene 168, 267-270], contains an open reading frame homologous to those of the DNase I family enzymes. The full open reading frame of this gene has been fused to the E. coli gene of the maltose binding protein and expressed in bacteria as a chimeric protein. The partially purified chimeric protein is enzymatically active. It introduces single and double stranded breaks into supercoiled DNA, at 30 degrees C in the absence of divalent cations and at a pH optimum of 5.2. To our knowledge this enzyme represents the first cloned human endonuclease with characteristics similar to those of acidic DNase II.
Appierto
V
V
Istituto Tecnologie Biomediche Avanzate (ITBA), CNR, Milano, Italy.
Bardella
L
L
Vijayasarathy
C
C
Avadhani
N
N
Sgaramella
V
V
Biunno
I
I
D003001:Cloning, Molecular; D004706:Endodeoxyribonucleases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli; D006801:Humans; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014960:X Chromosome
D011993:Recombinant Fusion Proteins; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II