title: Molecular characterization of the Entamoeba histolytica enolase gene and modelling of the predicted protein.
abstract: Entamoeba histolytica obtains its energy mainly from glucose fermentation. Enzymes involved in this pathway could be potential targets for antiparasite drugs. Here we report the molecular characterization of the E. histolytica enolase gene (Ehenl-I), which in a single copy is located on the 1.6 Mb chromosome. It is transcribed into a 1.4 kb mRNA which starts 13 nucleotides upstream of the ATG start codon. The sequence TATAAG, at -31, interacted with nuclear proteins suggesting that it has a TATA box function. Protein modelling allowed us to identify a putative specific region that differs from human enolase and could be a good target for the design of novel drugs against E. histolytica.
authors.affiliation: Departamento de Parología Experimental, Instituto Politécnico Nacional, México, D.F., Mexico.
authors.forename: M E
authors.initials: ME
authors.lastname: Hidalgo
authors.forename: R
authors.initials: R
authors.lastname: Sánchez
authors.forename: D G
authors.initials: DG
authors.lastname: Pérez
authors.forename: M A
authors.initials: MA
authors.lastname: Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: García
authors.forename: E
authors.initials: E
authors.lastname: Orozco
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics; D017125:Genes, Protozoan; D006801:Humans; D008961:Models, Structural; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000235:genetics*; D016385:TATA Box; D014158:Transcription, Genetic
chemical_list: D010751:Phosphopyruvate Hydratase