title: Interleukin-8 receptor B immunoreactivity in brain and neuritic plaques of Alzheimer's disease.
abstract: Cytokines mediate inflammatory responses through their receptors in the hematopoietic system. In a search for potential mediators of inflammatory responses in Alzheimer's disease, we examined brain for cytokine receptors. Herein we describe interleukin-8 receptor B (IL-8RB, also termed CXCR2) immunoreactivity in the central nervous system. Strong IL-8RB immunoreactivity is present in both Alzheimer's disease and control brains. Neurons, dendrites, and axons are clearly immunoreactive. In Alzheimer's disease, IL-8RB immunoreactivity is also present in some swollen dystrophic neurites of neuritic plaques. Double staining and confocal microscopic analysis reveals that these IL-8RB-positive neurites in plaques are neurofilament positive and are distinct from astrocytic or microglial processes. In general, these IL-8RB-positive neurities do not co-localize with PHF-1 or AT8 (hyperphosphorylated tau) immunoreactive neurites but instead co-localize with beta PP-positive neurites. These results demonstrate for the first time IL-8RB immunoreactivity in the central nervous system and imply a new role for this receptor outside the hematopoietic system. The strong presence of IL-8RB on neurons and the potential of glial cells to produce IL-8 suggest that this system might mediate neuronal-glial interactions.
authors.affiliation: Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Cambridge, USA.
authors.forename: M
authors.initials: M
authors.lastname: Xia
authors.forename: S
authors.initials: S
authors.lastname: Qin
authors.forename: M
authors.initials: M
authors.lastname: McNamara
authors.forename: C
authors.initials: C
authors.lastname: Mackay
authors.forename: B T
authors.initials: BT
authors.lastname: Hyman
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D001923:Brain Chemistry / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D018613:Microscopy, Confocal; D008875:Middle Aged; D016874:Neurofibrillary Tangles / Q000737:chemistry / Q000276:immunology*; D019707:Receptors, Chemokine / Q000276:immunology*; D018123:Receptors, Interleukin / Q000737:chemistry / Q000276:immunology*; D023063:Receptors, Interleukin-8B; D013194:Staining and Labeling; D013702:Temporal Lobe / Q000737:chemistry / Q000276:immunology
chemical_list: D016209:Interleukin-8; D019707:Receptors, Chemokine; D018123:Receptors, Interleukin; D023063:Receptors, Interleukin-8B