title: Feasibility of measuring blood glucose concentration by near-infrared Raman spectroscopy.
abstract: We report the determinations of glucose concentrations in human whole blood samples made using near-infrared Raman spectroscopy. Raman spectra of blood samples with above-physiological levels of glucose were acquired for 5 min through the wall of a cuvette via fiber optics. Partial least squares analysis was used to predict glucose concentrations in the samples. A root mean squared prediction error of 3.6 mM glucose was achieved with a correlation coefficient of 0.99 between reference and predicted values. This result is the first step in evaluating the potential of near-infrared Raman spectroscopy to perform blood glucose measurement with clinical accuracy. The technique is capable of measuring the concentration of other Raman-active blood constituents; as an example, bicarbonate was also measured. The method could eventually be useful for direct measurement of tissue analytes.
authors.affiliation: G. R. Harrison Spectroscopy Laboratory, MIT, Cambridge 02139, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Berger
authors.forename: I
authors.initials: I
authors.lastname: Itzkan
authors.forename: M S
authors.initials: MS
authors.lastname: Feld
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D001786:Blood Glucose / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006801:Humans; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
chemical_list: D001786:Blood Glucose