title: Mesothelial versus endothelial cell seeding: evaluation of cell adherence to a fibroblastic matrix using 111In oxine.
abstract: OBJECTIVE
  The aim of this study was to compare the behaviour of mesothelial cells (MC) to that of endothelial cells (EC) when seeded onto a PTFE, prosthesis coated with a fibroblastic matrix.
  
  
  METHODS
  Three study groups were examined: a control group (Control) of PTFE prostheses with a fibroblast matrix (n = 8); Group EC, PTFE prostheses seeded with EC on a fibroblastic matrix (n = 8); and Group MC, PTFE, prostheses seeded with MC on a fibroblastic matrix (n = 8). All cell types were labelled with 111In (100 microCi/ml) 24 h after seeding, when the cells had formed a monolayer on the prosthetic surface. Radioactive levels were measured at 2, 4, 6, and 24 h.
  
  
  RESULTS
  Both EC and MC showed optimal adherence. The MC had a better radioactive uptake and retention than the EC. The number of EC and MC cells that remained adherent to the matrix was large enough to ensure complete covering of the prosthetic surface.
  
  
  CONCLUSIONS
  The use of MC is therefore feasible as an optimal alternative for achieving a natural covering on vascular prostheses prepared with a fibroblastic matrix.
authors.affiliation: Department of Morphological Sciences and Surgery, University of Alcala de Henares, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Bellón
authors.forename: N
authors.initials: N
authors.lastname: García-Honduvilla
authors.forename: C
authors.initials: C
authors.lastname: Escudero
authors.forename: M J
authors.initials: MJ
authors.lastname: Gimeno
authors.forename: L
authors.initials: L
authors.lastname: Contreras
authors.forename: J
authors.initials: J
authors.lastname: de Haro
authors.forename: J
authors.initials: J
authors.lastname: Buján
mesh_terms: D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007205:Indium Radioisotopes; D009852:Omentum; D009942:Organometallic Compounds; D015125:Oxyquinoline / Q000031:analogs & derivatives; D011138:Polytetrafluoroethylene; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C032950:indium oxine; D015125:Oxyquinoline; D011138:Polytetrafluoroethylene