Effect of granulocyte colony stimulating factor on pig blood platelets treated with cisplatin in vitro.
The effects of granulocyte colony stimulating factor (G-CSF) and cisplatin in vitro on the peroxidation of pig platelet endogenous arachidonate were investigated. Estimation of the level of malonyldialdehyde (MDA) after stimulation of platelets with thrombin served as an indicator of this process. MDA concentration was determined by means of a modified method with thiobarbituric acid. The effects of G-CSF on thrombin-induced formation of MDA in platelets was dependent on the concentration and the time of platelet preincubation with this cytokine. In the platelets obtained from the whole blood preincubated with the highest concentration of G-CSF (8 microg/ml), a significant increase of MDA formation was observed (p<0.01). Cisplatin (20 microM) had a strong inhibitory effect on arachidonic acid metabolism in platelets (p<0.001). In the presence of G-CSF (0.8 microg/ml) in incubation medium the inhibitory effect of cisplatin on platelet arachidonate pathway was reduced. The results showed that G-CSF had a protective effect against cisplatin action on platelets.
Olas
B
B
Department of General Biochemistry, Institute of Biochemistry, University of Lódź, Banacha, Poland.
Wachowicz
B
B
Wachowicz
N
N
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D016718:Arachidonic Acid / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D002945:Cisplatin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism*; D013552:Swine; D013858:Thiobarbiturates / Q000737:chemistry; D013917:Thrombin / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism
D000970:Antineoplastic Agents; D013858:Thiobarbiturates; D016179:Granulocyte Colony-Stimulating Factor; D016718:Arachidonic Acid; D008315:Malondialdehyde; D013917:Thrombin; C029684:thiobarbituric acid; D002945:Cisplatin