Expression of H19 does not influence the timing of replication of the Igf2/H19 imprinted region.
Allele specific timing of replication is believed to be a hallmark of imprinted genes, however recent evidence suggests that this might not be the case for the insulin-like growth factor 2 (Igf2) and H19 locus. In this report, we assayed the timing of replication of Igf2 and H19 in two mouse embryonic cell lines expressing both H19 and Igf2, and one cell line maternally disomic for the Igf2/H19 mouse locus which expresses H19 but not Igf22. In all cell lines, Igf2 and H19 were replicated early in the S phase of the cell cycle, and both alleles replicated at the same time. This indicates that any differences in the timing of replication at the Igf2/H19 locus are of a lesser magnitude than those found in other imprinted regions.
Windham
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CQ
Department of Biochemistry and Molecular Biology, USC/Norris Comprehensive Cancer Center, Los Angeles, USA.
Jones
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PA
D000483:Alleles; D000818:Animals; D002460:Cell Line; D004261:DNA Replication; D005260:Female; D018392:Genomic Imprinting / Q000502:physiology*; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D051379:Mice; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics*; D009630:Nondisjunction, Genetic; D016133:Polymerase Chain Reaction; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D016196:S Phase; D013997:Time Factors
C094335:H19 long non-coding RNA; D009124:Muscle Proteins; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D007335:Insulin-Like Growth Factor II