Interleukin-10 in preovulatory follicular fluid of patients undergoing in-vitro fertilization and embryo transfer.
OBJECTIVE
The present study aimed at investigating the presence of Interleukin-10 (IL-10) in preovulatory follicular fluid (FF) and its possible correlation with 17-beta-estradiol (E2) and progesterone (P) levels, and treatment outcome in patients undergoing in-vitro fertilization-embryo transfer (IVF-ET).


METHODS
Twenty consecutive patients with tubal factor infertility who underwent oocyte retrieval for IVF-ET were assayed for pooled, preovulatory FF levels of IL-10, E2, and P.


RESULTS
The mean FF levels of IL-10, E2, and P were 78.7 +/- 104.7 pg/ml, 2,787.0 +/- 726.1 pg/ml, and 1.5 +/- 0.8 ng/ml, respectively. No correlation was found between preovulatory FF concentration of IL-10, E2, oocyte number, oocyte fertilization rate, embryo quality, and pregnancy rate. The levels of IL-10 were found to be negatively correlated with P concentration, although not significantly (P = 0.057).


CONCLUSIONS
Interleukin-10 exists in the preovulatory FF. Further investigations are needed to determine the role of IL-10 in the folliculogenesis.
Geva
E
E
In-Vitro Fertilization Unit, Serlin Maternity Hospital, Tel Aviv University, Israel.
Lessing
J B
JB
Lerner-Geva
L
L
Azem
F
F
Yovel
I
I
Ben-Yosef
D
D
Barkai
U
U
Amit
A
A
D000328:Adult; D004624:Embryo Transfer; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry / Q000378:metabolism*; D005498:Follicular Phase / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism
D016753:Interleukin-10; D011374:Progesterone; D004958:Estradiol