title: Generation and use of recombinant human bone sialoprotein and osteopontin for hydroxyapatite studies.
abstract: Bone sialoprotein (BSP) and osteopontin (OPN) are two extracellular bone matrix proteins that have the ability to modulate the growth of hydroxyapatite in vitro. Studies of BSP/OPN hydroxyapatite interactions in the past have been directed toward the identification of essential structural elements that allow these two proteins to modulate hydroxyapatite growth. However, these studies are limited by the finite quantities of purified extracellular matrix proteins. I have utilized a recombinant E. coli expression system to obtain milligram quantities of human bone sialoprotein and human osteopontin that may be used to study extracellular matrix protein interactions with hydroxyapatite.
authors.affiliation: Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Stubbs
mesh_terms: D017886:Durapatite / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D053495:Osteopontin; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; D011993:Recombinant Fusion Proteins; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D017886:Durapatite