The enigmatic plasmacytoid T cells develop into dendritic cells with interleukin (IL)-3 and CD40-ligand.
A subset of CD4+CD11c-CD3- blood cells was recently shown to develop into dendritic cells when cultured with monocyte conditioned medium. Here, we demonstrate that CD4+ CD11c-CD3- cells, isolated from tonsils, correspond to the so-called plasmacytoid T cells, an obscure cell type that has long been observed by pathologists within secondary lymphoid tissues. They express CD45RA, but not markers specific for known lymphoid- or myeloid-derived cell types. They undergo rapid apoptosis in culture, unless rescued by IL-3. Further addition of CD40-ligand results in their differentiation into dendritic cells that express low levels of myeloid antigens CD13 and CD33.
Grouard
G
G
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Rissoan
M C
MC
Filgueira
L
L
Durand
I
I
Banchereau
J
J
Liu
Y J
YJ
D015703:Antigens, CD / Q000032:analysis*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000032:analysis; D007377:Interleukin-3 / Q000494:pharmacology; D009000:Monocytes / Q000502:physiology; D014066:Palatine Tonsil / Q000276:immunology; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
D015703:Antigens, CD; D015415:Biomarkers; D017252:CD3 Complex; D015704:CD4 Antigens; D017077:Culture Media, Conditioned; D016167:Integrin alphaXbeta2; D007377:Interleukin-3