The isolation of a 22 kDa band after SDS-PAGE of Schistosoma mansoni adult worms and its use to demonstrate that IgE responses against the antigen(s) it contains are associated with human resistance to reinfection.
In schistosomiasis endemic areas, intensities of reinfection after treatment are greater amongst young children than amongst adults, and high levels of parasite-specific IgE are associated with resistance to reinfection in an age-dependent manner. Previously we have reported that, in Western blots, a 22 kDa band was recognized by human IgE and that the incidence and intensity of S. mansoni reinfection were significantly lower amongst individuals who had IgE against this band, compared with those who did not (Dunne et al. 1992). Here we report the isolation of a 22 kDa SDS-PAGE band, its incorporation into ELISA and the demonstration that levels of human anti-22 kDa IgE had a significant negative correlation with intensities of subsequent reinfection. Rabbit anti-22 kDa band serum recognized the outer tegument, gut tegument, and the collecting ducts and flame cells of adult worms. The 22 kDa band antigen(s) was also present in "lung'- and "post-lung' schistosomula stages of S. mansoni, and in S. haematobium, S. bovis and S. japonicum adult worms. Metabolic labelling of schistosomula and worms demonstrated the in vitro synthesis and release of 22 kDa antigens.
Dunne
D W
DW
Department of Pathology, Cambridge University, UK.
Webster
M
M
Smith
P
P
Langley
J G
JG
Richardson
B A
BA
Fulford
A J
AJ
Butterworth
A E
AE
Sturrock
R F
RF
Kariuki
H C
HC
Ouma
J H
JH
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000097:blood / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes; D012967:Sodium Dodecyl Sulfate; D007073:Immunoglobulin E