title: Enantioselective high-performance liquid chromatography assay of (+)R- and (-)S-alpha-lipoic acid in human plasma.
abstract: A specific plasma level assay for the enantiomers of alpha-lipoic acid is described. It makes use of liquid-liquid extraction, chemical reduction to the dithiol enantiomers, and their precolumn chiral derivatisation with o-phthalaldehyde in the presence of D-phenylalanine. The two diastereomeric derivatives are separated by reversed-phase HPLC with fluorescence detection. The working range of the assay is between 15 ng/ml (lower limit of quantitation) and 1,000 ng/ml for either enantiomer.
authors.affiliation: Department of Biochemistry, ASTA Medica AG, Frankfurt, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Niebch
authors.forename: B
authors.initials: B
authors.lastname: Büchele
authors.forename: J
authors.initials: J
authors.lastname: Blome
authors.forename: S
authors.initials: S
authors.lastname: Grieb
authors.forename: G
authors.initials: G
authors.lastname: Brandt
authors.forename: P
authors.initials: P
authors.lastname: Kampa
authors.forename: H H
authors.initials: HH
authors.lastname: Raffel
authors.forename: M
authors.initials: M
authors.lastname: Locher
authors.forename: H O
authors.initials: HO
authors.lastname: Borbe
authors.forename: I
authors.initials: I
authors.lastname: Nubert
authors.forename: I
authors.initials: I
authors.lastname: Fleischhauer
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D008063:Thioctic Acid / Q000097:blood*
chemical_list: D008063:Thioctic Acid