[The effect of a nerve growth factor from different biological sources on the spontaneous maturation of mouse oocytes and on the parthenogenetic activation of pronucleus formation].
The paracrine role of the nerve growth factor (NGF) in oocyte maturation is analysed. It is shown that the addition of mouse beta-NGF to the serum-free culture medium in doses of 100, 10 and 1 ng/ml induces reinitiation of meiosis in mouse oocytes after gonadotrophic stimulation. It is identified that oocytes without cumulus cells demonstrate a 2-3 fold increase in the rate of parthenogenetic pronucleus formation in comparison with the control. Similar results are obtained using bovine beta-NGF. It is supposed that the oocyte surface has a receptors to beta-NGF.
Fedorushchenko
A N
AN
Koval'
T Iu
TIu
Khamidov
D Kh
DKh
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000187:drug effects*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D008297:Male; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D010312:Parthenogenesis / Q000187:drug effects*; D013268:Stimulation, Chemical
D009414:Nerve Growth Factors; D004815:Epidermal Growth Factor; D003513:Cycloheximide
Vliianie neĭrorostovogo faktora iz razlichnykh biologicheskikh istochnikov na spontannoe sozrevanie ootsitov mysheĭ i partenogeneticheskuiu aktivatsiiu formirovaniia pronukleusa.