New chicken Rfp-Y haplotypes on the basis of MHC class II RFLP and MLC analyses.
New chicken Rfp-Y haplotypes were determined by the use of restriction fragment length polymorphism (RFLP) and mixed lymphocyte culture (MLC) in four different chicken haplotypes, B15, B19, B21, B201. The RFLP polymorphism was mapped to the Rfp-Y system by the use of a subclone (18.1) which maps near a polymorphic lectin gene located in the Rfp-Y system and DNA from families with known segregation of the implicated RFLP polymorphism. For the first time it is shown that major histocompatibility complex class II genes in the Rfp-Y system have functional implications. Sequence information of the B1 domain of the proposed Rfp-Y haplotypes was obtained which supported the functional data.
Juul-Madsen
H R
HR
Danish Institute of Animal Science, Research Centre Foulum, DK-8830 Tjele, Denmark.
Zoorob
R
R
Auffray
C
C
Skjodt
K
K
Hedemand
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002478:Cells, Cultured; D002645:Chickens; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length
D000949:Histocompatibility Antigens Class II