In vitro effects of recombinant human megakaryocyte growth and development factor on primary human tumour colony growth.
A study was made of the in vitro effects of recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF) on breast, prostate, cervix and colon cancers. Tumour growth was assessed using a soft agar clonogenic assay. Stimulation of megakaryocyte numbers was seen in liquid culture following 10 ng/ml PEG-rHuMGDF. The same concentration led to no significant difference in colony-forming efficiencies for 22 of 25 tumour samples. A significant reduction in colony-forming efficiencies was seen for the remaining 3 specimens. These results suggest that PEG-rHuMGDF will not stimulate tumour growth in vivo.
Lang
S H
SH
CRC Department of Experimental Haematology, Christie Hospital, Manchester, UK.
West
C M
CM
Jones
L
L
Brooks
B
B
Kasper
C
C
deWynter
E
E
Testa
N G
NG
D001943:Breast Neoplasms / Q000503:physiopathology; D002455:Cell Division; D002999:Clone Cells; D003110:Colonic Neoplasms / Q000503:physiopathology; D005260:Female; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000502:physiology*; D009369:Neoplasms / Q000503:physiopathology*; D011092:Polyethylene Glycols; D011471:Prostatic Neoplasms / Q000503:physiopathology; D011994:Recombinant Proteins; D013926:Thrombopoietin / Q000502:physiology*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000503:physiopathology
D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D013926:Thrombopoietin