title: The wheat protein kinase gene, TaPK3, of the PKABA1 subfamily is differentially regulated in greening wheat seedlings.
abstract: We have identified a new wheat PKABA1-like protein kinase gene, TaPK3, that is expressed in greening wheat seedlings. TaPK3 has high sequence homology (97% similarity with some sequence diversity at the 3' end) to the wheat PKABA1 protein kinase mRNA, which is upregulated by cold-temperature treatment, dehydration and abscisic acid (ABA). Use of a TaPK3 gene-specific probe has revealed that TaPK3 is differentially expressed with respect to PKABA1. TaPK3 mRNA accumulates in greening shoot tissue of wheat, but is not affected by dehydration, cold-temperature treatment or ABA. Based on sequence and expression differences, we conclude that expression of the PKABA1-like protein kinases is not limited to stress responses.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research, Washington State University, Pullman 99164-6420, USA.
authors.forename: L D
authors.initials: LD
authors.lastname: Holappa
authors.forename: M K
authors.initials: MK
authors.lastname: Walker-Simmons
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003624:Darkness; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D015698:Genomic Library; D008027:Light; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects
chemical_list: D010940:Plant Proteins; C105503:TaPK3 protein, Triticum aestivum; D017346:Protein Serine-Threonine Kinases