Expression of the F-type 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase mRNA during liver regeneration.
The expression of the F-type 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase mRNA was studied during liver regeneration by three independent assays: Northern blot analysis, reverse transcription-polymerase chain reaction and ribonuclease protection. We demonstrate the presence of F-type mRNA in foetal and adult rat livers and a transient increase in its levels with a maximum at 12 h after partial liver resection. The time course of F-type mRNA induction differs from that reported for the L-type isoform, suggesting differences in the regulation of the expression of F- and L-type isoforms of the bifunctional enzyme during liver regeneration.
Joaquin
M
M
Departament de Cièncias Fisiòlogiques Humanes i de la Nutrició, Universitat de Barcelona, Catalunya, Spain.
Rosa
J L
JL
Bartrons
R
R
Tauler
A
A
D000818:Animals; D015152:Blotting, Northern; D006498:Hepatectomy; D008115:Liver Regeneration; D008297:Male; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D017853:Phosphotransferases (Alcohol Group Acceptor); D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases