Immunolocalization of platelet-derived growth factor A and B chains and PDGF-alpha and beta receptors in human gingival wounds.
Platelet-derived growth factor (PDGF) is a polypeptide growth factor which has been implicated as a major mitogen involved in wound healing. The PDGF appears to promote periodontal regeneration; however, its distribution in gingival tissues is not known and how it participates in gingival wound healing is unclear. Using highly specific antibodies we have studied the distribution of PDGF A and B chains and alpha- and beta-PDGF receptors in healing human gingival wounds. Wounds were created by making a 0.75 mm deep incision in the papilla and healthy gingiva and biopsies were obtained from the same site after 8 h and 1, 3, 7, 14 and 21 d. Frozen sections were immunostained with affinity purified antibodies. The results showed that both epithelium and fibrin clot manifested positive immunostaining for anti-PDGF-A and B-chain antibodies. Staining was present in unwounded and wounded epithelia, and in the fibrin clot it appeared to be more intense for the PDGF-A chain. Blood vessels in connective tissue were also positive while other areas were largely negative. No significant staining was detectable in healthy tissues for anti-PDGF-alpha or -beta receptor antibodies. However, the wound site began to manifest positive immunostaining fro anti-beta-receptor antibody after 3 d of healing, became maximal at 7 d, and then decreased. Our data indicate, but do not prove, that gingival epithelium may be a source of PDGF A and B chains and that the A chain may have a more prominent role to play during early stages of healing. Expression of PDGF beta-receptor appears later at the wound site, indicating that the PDGF B isomer may regulate later wound healing events.
Green
R J
RJ
Department of Periodontics, University of Washington, Seattle 98195, USA.
Usui
M L
ML
Hart
C E
CE
Ammons
W F
WF
Narayanan
A S
AS
D000328:Adult; D000906:Antibodies; D000077214:Becaplermin; D001706:Biopsy; D001777:Blood Coagulation; D001808:Blood Vessels / Q000473:pathology; D004396:Coloring Agents; D003238:Connective Tissue / Q000473:pathology; D004848:Epithelium / Q000293:injuries / Q000473:pathology; D005337:Fibrin; D005500:Follow-Up Studies; D015870:Gene Expression; D005881:Gingiva / Q000293:injuries* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008934:Mitogens / Q000032:analysis; D010519:Periodontium / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000032:analysis* / Q000235:genetics; D020574:Proto-Oncogene Proteins c-sis; D017479:Receptors, Platelet-Derived Growth Factor / Q000032:analysis* / Q000235:genetics; D012038:Regeneration; D013997:Time Factors; D014945:Wound Healing
D000906:Antibodies; D004396:Coloring Agents; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; C075278:platelet-derived growth factor A; D000077214:Becaplermin; D005337:Fibrin; D017479:Receptors, Platelet-Derived Growth Factor