title: Antigen receptor engagement delivers a stop signal to migrating T lymphocytes.
abstract: We investigated the role of the T cell antigen receptor (TcR) in control of T cell migration in an in vitro system. We used T cells from transgenic mice bearing a TcR for the lysozyme peptide 48-62 bound to I-A(k) (3A9). T cells from the 3A9 TcR transgenic mice crawled on purified intercellular adhesion molecule-1 substrates, but strikingly, stopped upon interaction with the physiological ligand, i.e., the mouse I-A(k) with covalently attached hen egg white lysozyme peptide residues 48-62 complex. TcR-triggered stopping was reversible by treatment with adhesion-strengthening phorbol esters. The microtubule organizing center of stopped cells was positioned adjacent to the site of stable cell anchorage. Direct conversion of lymphocyte function associated-1 to the high-affinity conformation with antibodies also stopped T cells in a similar manner to antigen. Thus, physiological TcR engagement triggers a stop signal through lymphocyte function associated-1. We propose that the stop signal is an early and essential event in T cell activation that also will play an important role in control of T cell migration.
authors.affiliation: Center for Immunology and Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA. dustin@pathbox.wustl.edu
authors.forename: M L
authors.initials: ML
authors.lastname: Dustin
authors.forename: S K
authors.initials: SK
authors.lastname: Bromley
authors.forename: Z
authors.initials: Z
authors.lastname: Kan
authors.forename: D A
authors.initials: DA
authors.lastname: Peterson
authors.forename: E R
authors.initials: ER
authors.lastname: Unanue
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell