title: Large-scale production of class I bound peptides: assigning a signature to HLA-B*1501.
abstract: A peptide-based vaccine must be bound and presented by major histocompatibility complex class I molecules to elicit a CD8(+) T-cell response. Because class I HLA molecules are highly polymorphic, it has yet to be established how well a vaccine peptide that stimulates one individual's CD8(+) cytotoxic T lymphocytes will be presented by a second individual's different class I molecules. Therefore, to facilitate precise comparisons of class I peptide binding overlaps, we uniquely combined hollow-fiber bioreactors and mass spectrometry to assign precise peptide binding signatures to individual class I HLA molecules. In applying this strategy to HLA-B*1501, we isolated milligram quantities of B*1501-bound peptides and mapped them using mass spectrometry. Repeated analyses consistently assign the same peptide binding signature to B*1501; the degree of peptide binding overlap between any two class I molecules can thus be determined through comparison of their peptide signatures.
authors.affiliation: Department of Microbiology & Immunology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, OK 73190, USA.
authors.forename: K
authors.initials: K
authors.lastname: Prilliman
authors.forename: M
authors.initials: M
authors.lastname: Lindsey
authors.forename: Y
authors.initials: Y
authors.lastname: Zuo
authors.forename: K W
authors.initials: KW
authors.lastname: Jackson
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Hildebrand
mesh_terms: D003602:Cytotoxicity, Immunologic; D015235:HLA-B Antigens / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D015235:HLA-B Antigens; D010455:Peptides; D014614:Vaccines, Synthetic