C-anaphases (common variant) are unrelated to colchicine and phytohemagglutinin.
We evaluated the effect of different concentrations of colchicine (0.0, 0.1, 0.3, 0.5 microgram/ml) and phytohemagglutinin (PHA) (0.10, 0.15, 0.20 microgram/ml) on the rate of C-anaphases in lymphocyte cultures from five healthy individuals with the common variant of C-anaphases. For each of the 12 possible combinations, two subjects were randomly tested. The frequency of these variant figures was < 3%; a single-culture (out of six with the colchicine concentration of 0.1 microgram/ml) lacked C-anaphases. Multiple variance and Student's t tests revealed, as the only significant difference, a decrease with the colchicine concentration of 0.3 microgram/ml compared with the cultures without colchicine (p < 0.05), which exhibited the greatest ratio of C-anaphases. The PHA had no influence on the frequency of C-anaphases. We conclude that the common variant of C-anaphases is unrelated to the colchicine and PHA concentrations tested; moreover, our data confirm the occurrence of such a mitotic variant in colchicine-free cultures.
Domínguez
M G
MG
División de Genética, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, México.
Rivera
H
H
Troyo
R
R
D000328:Adult; D000705:Anaphase / Q000187:drug effects*; D002877:Chromosomes, Human / Q000648:ultrastructure; D003078:Colchicine / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D010835:Phytohemagglutinins / Q000494:pharmacology*; D008941:Spindle Apparatus / Q000187:drug effects* / Q000502:physiology
D010835:Phytohemagglutinins; D003078:Colchicine