Protein B23 is an important human factor for the nucleolar localization of the human immunodeficiency virus protein Tat.
Nucleolar shuttle protein B23 was found to bind to human immunodeficiency virus protein Tat, and this binding required the nucleolar localization motif of Tat. A fusion protein containing the B23 binding domain and beta-galactosidase caused mislocalization of Tat to the cytoplasm and inhibited the transactivation activity of Tat. These data suggest that B23 is a human factor necessary for the nucleolar localization of Tat.
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Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA. yiping@tmc.bcm.edu
D001665:Binding Sites; D002466:Cell Nucleolus / Q000378:metabolism*; D015696:Gene Products, tat / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006678:HIV / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000502:physiology*; D000090243:Nucleophosmin; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D001616:beta-Galactosidase / Q000494:pharmacology; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D000090243:Nucleophosmin; D001616:beta-Galactosidase