Cloning and sequencing of the gene encoding the plasma membrane H(+)-ATPase from an acidophilic red alga, Cyanidium caldarium.
A cDNA containing an open reading frame encoding the putative plasma membrane H(+)-ATPase in an acidophilic red alga, Cyanidium caldarium, was cloned and sequenced by means of PCR and Southern hybridization based on homologous sequences of P-type ATPases found in other organisms. The cloned cDNA is 3300 bp in length, containing a 2865 bp open reading frame encoding a polypeptide of 955 amino acids which has a predicted molecular mass of 105,371. The deduced amino acid sequence was found to be more homologous to those of P-type H(+)-ATPases from higher plants than that from the green alga Dunaliella bioculata.
Ohta
H
H
Department of Biology, Faculty of Science, Science University of Tokyo, Japan.
Shirakawa
H
H
Uchida
K
K
Yoshida
M
M
Matuo
Y
Y
Enami
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D005796:Genes; D008969:Molecular Sequence Data; D010802:Phylogeny; D006180:Proton-Translocating ATPases / Q000235:genetics*; D000461:Rhodophyta / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D006180:Proton-Translocating ATPases