Failure of D-fructose to stimulate protein biosynthesis in pancreatic islets.
Rat pancreatic islets were incubated for 90 min with L-[4-3H]phenylalanine in either the absence or presence of D-glucose and D-fructose. Relative to basal value, D-glucose (16.7 mM) increased the incorporation of the tritiated amino acid into trichloroacetic acid-precipitable material. However, when D-fructose was tested in the 80 to 240 mM concentration range, it failed to stimulate islet biosynthetic activity. Since D-fructose causes, in the same concentration range, a dose-related stimulation of insulin release, the dissociation between the biosynthetic and secretory responses to D-fructose supports the view that the insulinotropic action of the ketohexose does not entail the same metabolic determinants as those operative in glucose-stimulated islets.
Viñambres
C
C
Fundacion Jimenez Diaz, Madrid, Spain.
Villanueva-Peñacarrillo
M L
ML
Valverde
I
I
Malaisse
W J
WJ
D000818:Animals; D005260:Female; D005632:Fructose / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014238:Trichloroacetic Acid / Q000737:chemistry
D005632:Fructose; D014238:Trichloroacetic Acid; D005947:Glucose