The acetate kinase of Clostridum acetobutylicum strain P262.
Clostridum acetobutylicum strain P262 fermented glucose, pyruvate, or lactate, and the butyrate production was substrate-dependent. Differences in butyrate yield could not be explained by changes in butyrate kinase activities, but the butyrate production was inversely related to acetate kinase activity. The acetate kinase had a pH optimum of 8.0, a Km for acetate of 160 mM, and a kcat of 16, 800 min-1. The enyzme had a native molecular mass of 78 kDa; the size of 42 kDa on SDS-PAGE indicated that the acetate kinase of strain P262 was a homodimer.
Diez-Gonzalez
F
F
Agricultural Research Service, USDA and Section of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853, USA.
Russell
J B
JB
Hunter
J B
JB
D000084:Acetate Kinase / Q000302:isolation & purification / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism; D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D003013:Clostridium / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D009243:NAD / Q000032:analysis; D010709:Phosphate Acetyltransferase / Q000378:metabolism; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism
D000085:Acetates; D009243:NAD; D019344:Lactic Acid; D019289:Pyruvic Acid; D010709:Phosphate Acetyltransferase; D017851:Phosphotransferases (Carboxyl Group Acceptor); D000084:Acetate Kinase; C019285:butyrate kinase; D005947:Glucose