title: The role of type X collagen in endochondral ossification as deduced by Fourier transform infrared microscopy analysis.
abstract: Type X collagen has been implicated in the morphogenetic events of endochondral ossification (EO), including the calcification of hypertrophic cartilage and trabeculae prior to their replacement by bone and marrow. Recently, transgenic mice which expressed a truncated collagen X protein were reported to exhibit morphologic alterations in all tissues arising through EO. Fourier Transform InfraRed (FTIR) spectroscopy has previously been shown to provide quantitative and qualitative information about the relative amount of mineral and carbonate present, mineral composition, and crystal perfection. To determine the role of collagen X in mineralization, the "quality" of mineral crystals was analyzed in thin sections of calcified cartilage from tibia obtained from several independent transgenic mouse lines showing varying degrees of the mutant phenotype and mice without type X collagen expression, by means of Fourier Transform InfraRed microscopy (FTIRM). In the present paper, the term "mineral quality" is employed to describe crystallinity/crystal maturation, and acid phosphate content. The results indicate significant differences between normal and transgenic mice bone mineral, both in the amount present and the "quality" of the crystals. In contrast, the analysis of the mineral in mice without type X collagen expression was not different from their age/sex-matched controls.
authors.affiliation: Hospital for Special Surgery, Department of Ultrastructural Biochemistry, New York, NY 10021, USA.
authors.forename: E P
authors.initials: EP
authors.lastname: Paschalis
authors.forename: O
authors.initials: O
authors.lastname: Jacenko
authors.forename: B
authors.initials: B
authors.lastname: Olsen
authors.forename: B
authors.initials: B
authors.lastname: deCrombrugghe
authors.forename: A L
authors.initials: AL
authors.lastname: Boskey
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002356:Cartilage / Q000737:chemistry*; D003094:Collagen / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D010012:Osteogenesis; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003094:Collagen