title: A new 'two step' procedure for 4.5 log depletion of T and B cells in allogeneic transplantation and of neoplastic cells in autologous transplantation.
abstract: To evaluate a new 'two step' method for purging T, B and neoplastic cells from hematopoietic progenitor cells (PC), PCs were collected by apheresis and some suspensions were deliberately contaminated with 2-5% breast cancer cells. PCs were first processed through CellPro columns for positive selection of cells that express CD34. After this first step, the mean CD34+ cell recovery was 68 +/- 12%, and CD34+ cell purity was 61 +/- 11%; CD3+ and neoplastic cell depletion were 2.1 +/- 0.4 and 1.9 +/0 0.4 logs, respectively. Cells were further processed through the StemSep device for direct depletion of T and B cells or of breast cancer cells. After the first and the second step, overall CD34+ cell recovery was 50 +/- 7%, T and B cell removal was 4.7 +/- 0.4 log and neoplastic cell purging was 4.4 +/- 0.3 log, ie significantly superior to methods described in the past.
authors.affiliation: Division of Medical Oncology, IRCCS Maugeri Foundation, Pavia Medical Center, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bertolini
authors.forename: T
authors.initials: T
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: Battaglia
authors.forename: N
authors.initials: N
authors.lastname: Gibelli
authors.forename: P
authors.initials: P
authors.lastname: Pedrazzoli
authors.forename: G
authors.initials: G
authors.lastname: Robustelli della Cuna
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology; D001402:B-Lymphocytes / Q000473:pathology*; D016465:Bone Marrow Purging / Q000379:methods*; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018655:Lymphocyte Count; D008212:Lymphocyte Depletion; D013601:T-Lymphocytes / Q000473:pathology*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D014407:Tumor Cells, Cultured
chemical_list: D018952:Antigens, CD34