Brain microvascular endothelial cells and leukocytes derived from patients with multiple sclerosis exhibit increased adhesion capacity.
The adhesion properties of brain microvascular endothelial cells (MVEC) and leukocytes derived from patients with multiple sclerosis (MS) were investigated. Leukocytes and brain MVEC from MS patients exhibited significantly higher adhesion capacity than the same cells isolated from normal donors. Flow cytometry showed that MS-derived brain MVEC constitutively expressed higher levels of ICAM-1 and contained an increased proportion of MHC class II positive cells than normal brain MVEC. In contrast, no difference was seen for vascular cell adhesion molecule-1 and endothelial cell leukocyte adhesion molecule-1. Circulating leukocytes from MS patients expressed higher levels of LFA-1, a ligand of intercellular adhesion molecule-1 (ICAM-1), than did normal leukocytes. The data presented here suggest that the ICAM-1/LFA-1 interaction may determine cytoadherence of leukocytes to brain MVEC in MS.
Lou
J
J
Department of Anaesthesiology, Pharmacology, and Surgical Intensive Care, Hôpital Cantonal Universitaire, Centre Médical Universitaire (CMU), University of Geneva, Switzerland.
Chofflon
M
M
Juillard
C
C
Donati
Y
Y
Mili
N
N
Siegrist
C A
CA
Grau
G E
GE
D001921:Brain / Q000098:blood supply*; D002448:Cell Adhesion; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D005434:Flow Cytometry; D006681:HLA-D Antigens / Q000032:analysis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis; D007962:Leukocytes / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis / Q000096:biosynthesis; D008833:Microcirculation; D009103:Multiple Sclerosis / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D012016:Reference Values; D019108:Tight Junctions / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis
D006681:HLA-D Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1