title: Immunological cross-reactivity of the major allergen from perennial ryegrass (Lolium perenne), Lol p I, and the cysteine proteinase, bromelain.
abstract: Antibodies prepared in rabbits against the major allergen from ryegrass (Lolium perenne), Lol p I, cross-reacted with the cysteine proteinase bromelain from pineapple and vice versa. Deglycosylation of the proteins showed that the cross-reaction was based on recognition of the carbohydrate moiety of the allergen, but for bromelain the cross-reaction was most likely due to a combination of factors. The results indicate that the carbohydrate residues from these allergens play an important role in cross-reactions found between them and possibly those from other species.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602-7229, USA.
authors.forename: R N
authors.initials: RN
authors.lastname: Pike
authors.forename: D
authors.initials: D
authors.lastname: Bagarozzi
authors.forename: J
authors.initials: J
authors.lastname: Travis
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D052179:Antigens, Plant; D015153:Blotting, Western; D001963:Bromelains / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006031:Glycosylation; D008129:Lolium / Q000276:immunology*; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D011058:Pollen / Q000276:immunology; D011817:Rabbits
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C042814:Lol p I protein, Lolium perenne; D010940:Plant Proteins; D001963:Bromelains