A Drosophila gene with predicted rhoGEF, pleckstrin homology and SH3 domains is highly expressed in morphogenic tissues.
We have identified a Drosophila gene that has substantial sequence homology to a distinct class of proto-oncogenes that includes DBL, VAV, Tiam-1, ost and ect-2. It has predicted Rho or Rac guanine exchange factor (Rho/RacGEF) and pleckstin homology (PH) domains with the PH immediately downstream of the Rho/RacGEF. Rho/RacGEFs catalyze the dissociation of GDP from the Rho/Rac subfamily of Ras-like GTPases, thus activating the target Rho/Rac (Takai et al. (1995) Trends Biochem. Sci. 20, 227-231]. Members of the Rho/Rac subfamily regulate organization of the actin cytoskeleton, which controls the morphology, adhesion and motility of cells (Nobes et al. (1995) J. Cell Sci. 108, 225 233; Ridley and Hall (1992) Cell 70, 389-399; Ridley et al. (1992) Cell 70, 401-410]. Message from this gene is found throughout oogenesis and embryogenesis. Of particular interest, message is most abundant in furrows and folds of the embryo where cell shapes are changing and the cytoskeleton is likely to be undergoing reorganization.
Werner
L A
LA
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721, USA.
Manseau
L J
LJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000737:chemistry; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D017344:Genes, Insect; D020662:Guanine Nucleotide Exchange Factors; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D009698:Nucleoproteins / Q000737:chemistry; D009866:Oogenesis; D010750:Phosphoproteins; D063150:Polycomb Repressive Complex 1; D011506:Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D064067:Rho Guanine Nucleotide Exchange Factors; D016415:Sequence Alignment; D017421:Sequence Analysis; D020663:ras Guanine Nucleotide Exchange Factors; D018909:src Homology Domains
D001798:Blood Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D020662:Guanine Nucleotide Exchange Factors; D009698:Nucleoproteins; D010750:Phosphoproteins; D011506:Proteins; D012333:RNA, Messenger; D064067:Rho Guanine Nucleotide Exchange Factors; C072820:ph-d protein, Drosophila; C039077:platelet protein P47; D020663:ras Guanine Nucleotide Exchange Factors; C577401:rtGEF protein, Drosophila; D063150:Polycomb Repressive Complex 1; D019204:GTP-Binding Proteins