Volume regulation of spermatozoa by quinine-sensitive channels.
Bovine spermatozoa were shown to exhibit rapid regulatory volume decrease (RVD) when exposed to hypotonic saline media. This quinine- and quinidine-sensitive regulatory volume decrease was coincident with K+ release due to stretch-activation of inhibitor-specific presumptive K+ channels. The regulatory volume decrease response was much faster than a similar phenomenon observed in human peripheral blood lymphocytes. Studies on volume changes in different electrolyte and nonelectrolyte media suggested that: (1) this inhibitor-specific channel could also be a nonspecific pore in the spermatozoal membrane for nonelectrolytes below 150 daltons; (2) subpopulations (of nearly equal size) of the spermatozoa differ in the expression of the pore; (3) capacitation abolishes this distinction between subpopulations of spermatozoa; and (4) the general case of RVD for other mammalian spermatozoa was also established.
Kulkarni
S B
SB
Department of Biotechnology, University of Pune, Ganeshkhind, India.
Sauna
Z E
ZE
Somlata
V
V
Sitaramam
V
V
D000818:Animals; D002020:Buffaloes; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D008297:Male; D009994:Osmolar Concentration; D010101:Oxygen Consumption / Q000187:drug effects; D015221:Potassium Channels / Q000187:drug effects* / Q000378:metabolism; D011803:Quinine / Q000494:pharmacology*; D013075:Sperm Capacitation / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism
D003470:Culture Media; D015221:Potassium Channels; D011803:Quinine