title: Structure of a product complex of spinach ribulose-1,5-bisphosphate carboxylase/oxygenase.
abstract: The crystal structure of an activated complex of ribulose-1,5-bisphosphate carboxylase/oxygenase from spinach and its product 3-phosphoglycerate has been determined to 2.2 A resolution. The structure is of the open form with the active site accessible to the solvent as observed in the structures of the activated ligand-free enzyme and the complex of the activated enzyme with the substrate ribulose-1,5-bisphosphate. Two molecules of 3-phosphoglycerate are bound per active site. The phosphates of both molecules bind approximately at the same position as the phosphates of ribulose-1,5-bisphosphate or the six-carbon intermediate analogue 2-carboxyarabinitol-1,5-bisphosphate, but one product molecule is swung out from the active site with its carboxylate group pointing toward solution. The present structure points to direct participation of the active site side chain of lysine 175 in later stages of catalysis. This possibility is discussed in the light of mutagenesis studies.
authors.affiliation: Department of Molecular Biology, Swedish University of Agricultural Scineces, Uppsala.
authors.forename: T C
authors.initials: TC
authors.lastname: Taylor
authors.forename: I
authors.initials: I
authors.lastname: Andersson
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D005988:Glyceric Acids / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D008274:Magnesium / Q000737:chemistry; D008958:Models, Molecular; D010428:Pentosephosphates / Q000378:metabolism; D011485:Protein Binding; D019289:Pyruvic Acid / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000737:chemistry* / Q000378:metabolism; D012274:Ribulosephosphates / Q000378:metabolism; D018724:Spinacia oleracea / Q000201:enzymology; D013402:Sugar Alcohols / Q000378:metabolism
chemical_list: C102131:2-carboxy-3-ketoarabinitol 1,5-bisphosphate; D005988:Glyceric Acids; D010428:Pentosephosphates; D012274:Ribulosephosphates; D013402:Sugar Alcohols; C001933:ribulose-1,5 diphosphate; C005156:3-phosphoglycerate; D019289:Pyruvic Acid; D012273:Ribulose-Bisphosphate Carboxylase; D008274:Magnesium