title: [The isolation and characteristics of a chemically modified allergen (allergoid) from Dermatophagoides pteronyssinus mites].
abstract: Chemically modified allergens (allergoids) were obtained from house dust mites (Dermatophagoides pteronyssinus). The allergenicity of allergoids in comparison with commercial mite allergen was determined by the skin prick test and by the in vitro test of degranulation of passively sensitized rat mast cells. Changes in allergoid molecules were determined with the use of gel filtration in a column packed with Sephadex G-75, isoelectric focusing in polyacrylamide gel, the determination of the concentration of end amino groups and the enzymatic activity of preparations, as well as HPLC. The possibility of using these methods for controlling the process of modification was studied. The results confirmed the fact that the modification on the allergenic preparation really occurred and the allergenic activity of allergoids was low.
authors.affiliation: National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria.
authors.forename: B
authors.initials: B
authors.lastname: Petrunov
authors.forename: G
authors.initials: G
authors.lastname: Nikolov
authors.forename: D
authors.initials: D
authors.lastname: Konstantinova
authors.forename: T
authors.initials: T
authors.lastname: Antonova
authors.forename: R
authors.initials: R
authors.lastname: Iankova
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000080384:Allergoids; D000818:Animals; D015550:Cell Degranulation / Q000276:immunology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004306:Dose-Response Relationship, Immunologic; D005557:Formaldehyde; D006801:Humans; D008407:Mast Cells / Q000502:physiology; D008722:Methods; D008925:Mites / Q000276:immunology*; D010936:Plant Extracts / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D051381:Rats; D012882:Skin Tests; D014466:Ultraviolet Rays
chemical_list: D000485:Allergens; D000080384:Allergoids; D010936:Plant Extracts; D005557:Formaldehyde
vernacular_title: Poluchenie i kharakteristika khimicheski modifitsirovannogo allergena (allergoida) iz kleshcheĭ Dermatophagoides pteronyssinus.