Upregulation of HLA class-I gene transcription in K562 cells by analogs of splenopentin (SP-5)
We report here the capacity of three analogs of Splenopentin, to up regulate transcription of the HLA-B7 gene in the K562 cell line. These cells normally do not transcribe the HLA class I genes. The Splenopentin analogs used in this study are effective at very low molar concentrations and are non-toxic to cells at these levels. Electrophoretic Mobility Shift assays indicate that this transcriptional up regulation of HLA class I genes may be related to the appearance of novel class I promoter binding factors induced in the nuclei of treated cells.
Chatterjee-Kishore
M
M
Dept. of Medical Genetics, SGPGIMS, Lucknow, India.
Agrawal
S
S
Singh
V K
VK
Mathur
K B
KB
Haq
A
A
Agarwal
S S
SS
D002467:Cell Nucleus / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000032:analysis; D005786:Gene Expression Regulation / Q000187:drug effects; D005805:Genes, MHC Class I; D015793:HLA-B7 Antigen / Q000235:genetics*; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology*; D013946:Thymopoietins / Q000494:pharmacology*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D004268:DNA-Binding Proteins; D015793:HLA-B7 Antigen; D010446:Peptide Fragments; D013946:Thymopoietins; C041898:splenopentin