title: Active and alpha-1 proteinase inhibitor complexed leukocyte elastase levels in crevicular fluid from patients with periodontal diseases.
abstract: Human leukocyte elastase is present in large amounts in the crevicular fluid of patients with periodontal disease and was considered as a putative biological marker of the evolution of such diseases. The aim of this work was to measure spectrophotometrically amounts of active elastase (AE) and elastase complexed to alpha 1 proteinase inhibitor (E-alpha 1-PI) in gingival crevicular fluid obtained, from patients suffering from rapidly progressive periodontitis (RPP group) or adult periodontitis (AP group) with different probing depths (3 to 5 mm and > 6 mm). AE and E-alpha 1-PI concentrations were negligible in healthy individuals. AE, but not E-alpha 1-PI, concentration appears to vary significantly with the probing depth in patients suffering either from rapidly progressive or adult periodontitis. No correlations were found between levels of AE and E-alpha 1-PI in the different groups of patients. AE concentration seems to be a marker of periodontal diseases in relation with probing depth.
authors.affiliation: Département de Pharmacologie, Hôpital Cochin, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Meyer
authors.forename: F
authors.initials: F
authors.lastname: Guessous
authors.forename: C
authors.initials: C
authors.lastname: Huynh
authors.forename: G
authors.initials: G
authors.lastname: Godeau
authors.forename: W
authors.initials: W
authors.lastname: Hornebeck
authors.forename: J P
authors.initials: JP
authors.lastname: Giroud
authors.forename: M
authors.initials: M
authors.lastname: Roch-Arveiller
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D019272:Leukocyte Elastase / Q000032:analysis* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000201:enzymology / Q000473:pathology; D010518:Periodontitis / Q000201:enzymology* / Q000473:pathology; D015842:Serine Proteinase Inhibitors / Q000032:analysis* / Q000737:chemistry; D013053:Spectrophotometry; D000515:alpha 1-Antitrypsin / Q000032:analysis* / Q000737:chemistry
chemical_list: D015415:Biomarkers; D015842:Serine Proteinase Inhibitors; D000515:alpha 1-Antitrypsin; D019272:Leukocyte Elastase