title: Persistence of parvovirus B19 DNA in synovial membranes of young patients with and without chronic arthropathy.
abstract: BACKGROUND
  Human parvovirus B19 replicates in erythroid precursors of the bone marrow, and several diseases have been attributed to this virus including some cases of juvenile chronic arthropathy.
  
  
  METHODS
  Tissue samples from children with juvenile arthritis and from healthy young adults with recent joint trauma were examined for B19 DNA by PCR. We also studied the timing of the parvovirus infection serologically.
  
  
  RESULTS
  All samples of synovial fluid, bone marrow, and blood were negative for B19 DNA. Eight (28%) of the 29 children with chronic arthritis had B19 DNA in synovial tissues. However, an even higher proportion of the non-arthropathy controls were positive for B19 DNA in synovial membranes (13 [48%] of 27). All the individuals with B19 DNA in synovial membrane had serum IgG antibodies to B19.
  
  
  CONCLUSIONS
  Genomic B19 DNA can persist in the synovial membranes not only in patients with chronic arthropathy but also in healthy immunocompetent individuals. The diagnostic criteria for parvovirus arthropathy must be reevaluated.
authors.affiliation: Department of Virology, Haartman Institute, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Söderlund
authors.forename: R
authors.initials: R
authors.lastname: von Essen
authors.forename: J
authors.initials: J
authors.lastname: Haapasaari
authors.forename: U
authors.initials: U
authors.lastname: Kiistala
authors.forename: O
authors.initials: O
authors.lastname: Kiviluoto
authors.forename: K
authors.initials: K
authors.lastname: Hedman
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D001171:Arthritis, Juvenile / Q000821:virology*; D001853:Bone Marrow / Q000821:virology; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D006801:Humans; D016732:Parvovirus B19, Human / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013583:Synovial Membrane / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral