nm23-H4, a new member of the family of human nm23/nucleoside diphosphate kinase genes localised on chromosome 16p13.
A novel human nm23/nucleoside diphosphate (NDP) kinase gene, called nm23-H4, was identified by screening a human stomach cDNA library with a probe generated by amplification by reverse transcription-polymerase chain reaction. The primers were designed from publicly available database cDNA sequences selected according to their homology to the human nn23-H1 putative metastasis suppressor gene. The full-length cDNA sequence predicts a 187 amino acid protein possessing the region homologous to NDP kinases with all residues crucial for nucleotide binding and catalysis, strongly suggesting that Nm23-H4 possesses NDP kinase activity. It shares 56, 55 and 60% identity with Nm23-H1, Nm23-H2 and DR-Nm23, respectively, the other human Nm23 proteins isolated so far. Compared with these proteins, Nm23-H4 contains an additional NH2-terminal region that is rich in positively charged residues and could indicate routing to mitochondria. The nm23-H4 gene has been localised to human chromosomal band 16p13.3. The corresponding 1.2 kb mRNA is widely distributed and expressed in a tissue-dependent manner, being found at very high levels in prostate, heart, liver, small intestine and skeletal muscle tissues and in low amounts in the brain and in blood leucocytes. Nm23-H4 naturally possesses the Pro-Ser substitution equivalent to the K-pn mutation (P97S) of Drosophila.
Milon
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L
INSERM U 402, Faculté de Médecine Saint-Antoine, Paris, France.
Rousseau-Merck
M F
MF
Munier
A
A
Erent
M
M
Lascu
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I
Capeau
J
J
Lacombe
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins; D005810:Multigene Family; D054778:NM23 Nucleoside Diphosphate Kinases; D061107:Nucleoside Diphosphate Kinase D; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
D018076:DNA, Complementary; D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; C517938:NME1 protein, human; C517946:NME4 protein, human; D061107:Nucleoside Diphosphate Kinase D; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins