title: Refractive-index detection by interferometric backscatter in packed-capillary high-performance liquid chromatography.
abstract: A simple, inexpensive, micro-volume refractive index detector has been applied to packed capillary high-performance liquid chromatography. Nanoliter detector volumes produced by a unique optical train, based on interferometric backscatter, allows for universal solute detection at the picogram level. System utility is demonstrated by reversed-phase separation of a test mixture containing ppm levels of NaCl, phenol, ethyl benzene and toluene.
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409-1061, USA.
authors.forename: C K
authors.initials: CK
authors.lastname: Kenmore
authors.forename: S R
authors.initials: SR
authors.lastname: Erskine
authors.forename: D J
authors.initials: DJ
authors.lastname: Bornhop
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity