Refractive-index detection by interferometric backscatter in packed-capillary high-performance liquid chromatography.
A simple, inexpensive, micro-volume refractive index detector has been applied to packed capillary high-performance liquid chromatography. Nanoliter detector volumes produced by a unique optical train, based on interferometric backscatter, allows for universal solute detection at the picogram level. System utility is demonstrated by reversed-phase separation of a test mixture containing ppm levels of NaCl, phenol, ethyl benzene and toluene.
Kenmore
C K
CK
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409-1061, USA.
Erskine
S R
SR
Bornhop
D J
DJ
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity