[A theoretical analysis of the primary structure of the yeast SEC59 gene product. Topology in the membrane, identification of the repetitive sequences and internal symmetry].
In this investigation the results of the theoretical analysis of primary structure of yeast gene SEC59 product, which possesses the dolichol kinase activity, were represented. The amino acid sequences (AAS), potentially penetrating the membrane, were identified by using of hydropathic and hydrophobic analyses. The some of these sequences were homologous. The longer symmetrical AAS with centres of symmetry in dolichol-binding region and related sequences were identified by using of comparative analysis of amino acid codon roots. The graphic method of analysis of amino acid codon roots sequences was used for identification of repeating segments in primary structure of SEC59 molecule. These segments contain less than 18 amino acid residues and construct the main part of polypeptide chain (for exclusion of N-terminal region of SEC59).
Shpakov
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D000595:Amino Acid Sequence; D003062:Codon / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D003062:Codon; D005656:Fungal Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); C020087:dolichol kinase
Teoreticheskiĭ analiz pervichnoĭ struktury produkta gena SEC59 drozhzheĭ. Topologiia v membrane, identifikatsiia povtoriaiushchikhsia posledovatel'nosteĭ i vnutrenneĭ simmetrii.