title: Calmodulin content and in vitro contractility of duodenum from streptozotocin-induced diabetic rats: effects of insulin therapy and calmodulin antagonism.
abstract: The effects of experimental diabetes and insulin treatment on the decreased reactivity of isolated rat duodenum to KCl and calmidazolium, a specific calmodulin antagonist, were examined. After 8 weeks of streptozotocin diabetes, the contractile effect of KCl and the non-competitive antagonistic effect of calmidazolium against KCl on isolated rat duodenum were decreased. Calmodulin levels, as measured by radioimmunoassay, were also found to the decreased in duodenum from streptozotocin-diabetic rats. Neither impaired reactivity to KCl nor decreased calmodulin levels in diabetic rat duodenum were corrected by treatment with insulin (10 IU/kg for 20 days). Following insulin treatment, there was only a partial correction in the antagonistic effect of calmidazolium as shown by the increase in non-competitive antagonist affinity constant.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Anadolu University, Ankara, Turkey. YOZTURK@vm.baum.anadolu.edu.tr
authors.forename: Y
authors.initials: Y
authors.lastname: Oztürk
authors.forename: S
authors.initials: S
authors.lastname: Aydin
authors.forename: A T
authors.initials: AT
authors.lastname: Ozçelikay
authors.forename: V M
authors.initials: VM
authors.lastname: Altan
authors.forename: N
authors.initials: N
authors.lastname: Yildizoğlu-Ari
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology*; D004386:Duodenum / Q000187:drug effects* / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D066298:In Vitro Techniques; D007328:Insulin / Q000097:blood / Q000627:therapeutic use*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D002147:Calmodulin; D007004:Hypoglycemic Agents; D007328:Insulin; D011189:Potassium Chloride