The correlation between adhesion of schizophyllan to yeast glucan and its effect on regeneration of yeast protoplast.
Schizophyllan, a water-soluble (1-->3)-beta-D-glucan with a triple-helical conformation, adheres to yeast glucan and curdlan gel. As the molecular weight of schizophyllan decreases, both its adhesion to the water-insoluble glucans and its ability to promote the regeneration of yeast protoplasts are reduced. Therefore, we hypothesize that schizophyllan can surround yeast protoplasts by adhering to a fragment of yeast glucan remaining or/and resynthesized on the cell surface and that this encapsulation allows regeneration of the protoplast cells to occur at very high frequency.
Hisamatsu
M
M
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Tsu, Japan.
Mishima
T
T
Teranishi
K
K
Yamada
T
T
D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000378:metabolism; D005936:Glucans / Q000378:metabolism*; D008970:Molecular Weight; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology*; D012567:Schizophyllum / Q000737:chemistry; D012566:Sizofiran / Q000378:metabolism* / Q000494:pharmacology*; D013053:Spectrophotometry; D014961:X-Ray Diffraction; D047071:beta-Glucans
D005936:Glucans; D047071:beta-Glucans; C038459:curdlan; D012566:Sizofiran