title: High incidence of Kaposi's sarcoma-associated herpesvirus and Epstein-Barr virus in tumor lesions and peripheral blood mononuclear cells from patients with Kaposi's sarcoma in Uganda.
abstract: With the advent of AIDS, Kaposi's sarcoma (KS) has become one of the leading malignancies in sub-Saharan Africa. Recently, DNA sequences from a new human herpesvirus called KS-associated herpesvirus (KSHV) or human herpesvirus type 8 have been found in KS tumor lesions in high frequency. Analyses of tumor lesions from 38 Ugandan KS patients indicated a uniform presence of KSHV in KS tumor lesions as revealed by polymerase chain reaction and Southern hybridization. In contrast, only 31% (11/36) of the normal skin biopsies from the same patient population were positive. The frequency of KSHV DNA detection in peripheral blood mononuclear cells (PBMC) of KS patients was also high (84%, 31/37). Similar analyses revealed the presence of cytomegalovirus (21% in KS lesions) to be discordant with KS development. A large number of KS lesions (87%, 33/38) and KS PBMC (70%, 26/37) were, however, positive for Epstein-Barr virus sequences. In addition, KSHV DNA was not found in the PBMC of Ugandans without KS.
authors.affiliation: Department of Medicine, Case Western Reserve University School of Medicine, and University Hospitals of Cleveland, Ohio, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Purvis
authors.forename: E
authors.initials: E
authors.lastname: Katongole-Mbidde
authors.forename: J L
authors.initials: JL
authors.lastname: Johnson
authors.forename: D G
authors.initials: DG
authors.lastname: Leonard
authors.forename: N
authors.initials: N
authors.lastname: Byabazaire
authors.forename: C
authors.initials: C
authors.lastname: Luckey
authors.forename: H E
authors.initials: HE
authors.lastname: Schick
authors.forename: R
authors.initials: R
authors.lastname: Wallis
authors.forename: C A
authors.initials: CA
authors.lastname: Elmets
authors.forename: C Z
authors.initials: CZ
authors.lastname: Giam
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006564:Herpesviridae / Q000302:isolation & purification*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000821:virology*
chemical_list: D004279:DNA, Viral