title: Detection of Salmonella spp. in food products by polymerase chain reaction and hybridization assay in microplate format.
abstract: Here, hybridization assay of amplified products is described which detect Salmonella spp. from chicken fillets and other food homogenates within 24 h. This technique is composed of four steps: (1) sample is pre-enriched overnight in phosphate buffered peptone water; (2) total DNA is extracted; (3) a Salmonella spp. specific DNA target sequence is amplified by polymerase chain reaction; (4) amplified products are captured by a probe covalently bound onto NH-Covalink (Nunc, Danemark) microwells and detected by a chemiluminescent enzymatic reaction. This hybridization of amplified products was demonstrated as sensitive as their analysis on agarose gel. Compared to a bacteriological method for Salmonella spp. detection, its specificity was estimated at 100% and its sensitivity was 93.2% from analysis of 207 naturally contaminated chicken fillets samples.
authors.affiliation: Unité de Recherches de Biologie Moléculaire, CNEVA Ploufragan, Zoopôle Les Croix, France.
authors.forename: C
authors.initials: C
authors.lastname: Soumet
authors.forename: G
authors.initials: G
authors.lastname: Ermel
authors.forename: G
authors.initials: G
authors.lastname: Salvat
authors.forename: P
authors.initials: P
authors.lastname: Colin
mesh_terms: D000818:Animals; D002645:Chickens; D005516:Food Microbiology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000302:isolation & purification*