Colorimetric detection of immobilised PCR products generated on a solid support.
By extending functional primers attached to a solid phase and incorporating a digoxigenin label, it is possible to visualise PCR products as discrete spots on specific regions of a solid support after colorimetric detection. The technique has been used for the detection of the point mutation associated with porcine malignant hyperthermia.
Lockley
A K
AK
Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics LE12 5RD, UK. andrew.lockley@nottingham.ac.uk
Jones
C G
CG
Bruce
J S
JS
Franklin
S J
SJ
Bardsley
R G
RG
D000483:Alleles; D001483:Base Sequence; D003124:Colorimetry / Q000379:methods; D017931:DNA Primers / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D016373:DNA, Antisense / Q000138:chemical synthesis / Q000737:chemistry; D004076:Digoxigenin; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D016373:DNA, Antisense; D007202:Indicators and Reagents; D004076:Digoxigenin