Reverse transcriptase-like activity in Trypanosoma cruzi.
The use of specific synthetic RNA homopolymers as templates and short oligonucleotides as primers has allowed evidence of the existence of a reverse transcriptase-like activity in Trypanosoma cruzi, to be revealed. The RNA:DNA products derived from this reaction are of approximately 110 nucleotides in length. The enzyme has greater affinity for poly(rA)/ oligo(dT) templates than for poly(rC)/oligo(dG) having a 20 mM Mg+2 ion requirement. The detected reverse transcriptase-like activity is not affected by aphidicolin and ddTTP but is inhibited by actinomycin D. novobiocin, rifamycin SV and AZT.
González
C I
CI
Departamento de Biología Molecular, Instituto de Parasitologia y Biomedicina, Lopez Negra CSIC, Granada, Spain.
Thomas
M C
MC
Martín
F
F
Alcami
J
J
Alonso
C
C
López
M C
MC
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D054306:Dideoxynucleotides; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011061:Poly A / Q000235:genetics; D011066:Poly C / Q000235:genetics; D011068:Poly G / Q000235:genetics; D011071:Poly T / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D012294:Rifamycins / Q000494:pharmacology; D013942:Thymine Nucleotides / Q000494:pharmacology; D014158:Transcription, Genetic; D014345:Trypanosoma / Q000235:genetics*; D015215:Zidovudine / Q000494:pharmacology
D054306:Dideoxynucleotides; D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D016053:RNA, Protozoan; D018894:Reverse Transcriptase Inhibitors; D012294:Rifamycins; D013942:Thymine Nucleotides; D003609:Dactinomycin; D011061:Poly A; D011071:Poly T; D011068:Poly G; C008961:poly G-poly C; C008950:poly A-T; D011066:Poly C; D016590:Aphidicolin; D015215:Zidovudine; C023808:rifamycin SV; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium; C017216:2',3'-dideoxythymidine triphosphate