Protein kinase C activation in rat colonic mucosa after diets differing in their fatty acid composition.
We studied the effects of different types of dietary fats on fatty acid composition and activity of protein kinase C (PKC) in rat colonic mucosa. Activation of PKC, a key enzyme in signal transduction and growth regulation, provides a mechanism by which dietary components could be involved in colon carcinogenesis. Male Wistar rats (n = 12/group) were fed a semisynthetic high fat diet (43% of energy) containing either sunflower oil, rapeseed oil, or butter for 4 weeks ad libitum. The control group received a low fat sunflower oil diet (10% of energy). The butter diet increased membrane-associated PKC activity in rat colonic mucosa compared with the low fat control diet (1237 vs. 917 pmol/min per mg prot.; P = 0.028). Mucosal fatty acids reflected dietary fatty acid composition even though there was no clear association between the amount of mucosal fatty acids and PKC activity. More research is needed to elucidate how dietary fatty acids regulate colonic PKC activity.
Pajari
A M
AM
Department of Applied Chemistry and Microbiology, University of Helsinki, Finland.
Rasilo
M L
ML
Mutanen
M
M
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology*; D004789:Enzyme Activation; D005227:Fatty Acids; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D004041:Dietary Fats; D005227:Fatty Acids; D011493:Protein Kinase C