Polymorphism of bovine MHC class I genes. Joint report of the Fifth International Bovine Lymphocyte Antigen (BoLA) Workshop, Interlaken, Switzerland, 1 August 1992.
The objectives of the Fifth International BoLA Workshop were to: standardize nomenclature, compare typing methods, and characterize BoLA haplotypes. The workshop was based on the distribution of blood samples (cells) from 60 selected cattle to 14 laboratories. Results for the class I (BoLA-A) region are presented in this paper while results for the class II regions are presented in a separate report. Thirty-six of the 50 previously established serological class I specificities were represented in the cell panel. However, only 30 specificities could be confirmed. Two specificities, A16 and A32, were upgraded from provisional, workshop (w) specificities to BoLA-A locus specificities and three new specificities, w51(w28), w52 and w53(w28), were defined. The 39 specificities distinguished 30 class I haplotypes in the 60 animals. Class I isoelectric focusing proved to be a useful adjunct to the serology. Isoelectric focusing confirmed several serologically defined splits and detected splits of A15(A8), A18(A6) and A22(w49) that had not been detected by serology. Subsequently, serological support for splits of A15(A8) and A22(w49) was found.
Davies
C J
CJ
Department of Microbiology, Immunology and Parasitology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA.
Joosten
I
I
Bernoco
D
D
Arriens
M A
MA
Bester
J
J
Ceriotti
G
G
Ellis
S
S
Hensen
E J
EJ
Hines
H C
HC
Horin
P
P
D000818:Animals; D001789:Blood Group Antigens; D002417:Cattle / Q000235:genetics* / Q000276:immunology*; D005260:Female; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000302:isolation & purification; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000302:isolation & purification; D007525:Isoelectric Focusing; D008297:Male; D011110:Polymorphism, Genetic; D012703:Serotyping; D009626:Terminology as Topic
D001789:Blood Group Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II