T-DNA insertion mutagenesis in Arabidopsis: going back and forth.
T-DNA insertion mutagenesis in Arabidopsis has been instrumental in advancing our knowledge of the physiology, biochemistry and development of plants. In just a few years plant biology, the late starter of molecular genetics, has placed itself at the forefront of science in large measure as a result of the more than 20000 T-DNA transformants that have been generated in Arabidopsis. To date, approximately 4000 mutants have been identified and more than 40 genes have been isolated via the T-DNA tag. The large population of transformed lines in now being subjected to a PCR-based reverse genetics strategy to identify new insertion mutants in sequences of interest.
Azpiroz-Leehan
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Department of Biochemistry, University of Arizona, Tucson, AZ 85721, USA. azpiroz@u.arizona.edu
Feldmann
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D017360:Arabidopsis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D017343:Genes, Plant; D016254:Mutagenesis, Insertional; D010641:Phenotype; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000235:genetics
D004269:DNA, Bacterial; C036483:T-DNA