title: Enhancement of cell-mediated immunity against HIV-1 induced by coinnoculation of plasmid-encoded HIV-1 antigen with plasmid expressing IL-12.
abstract: Previous investigations have demonstrated that CTL may play an important role in suppressing the disease progression of HIV infection. In this study, we inoculated mice with IL-12 expression plasmid together with plasmid-encoding HIV-1 envelope to enhance CTL activity by activating a Th1-type response. The results of delayed-type hypersensitivity using the footpad swelling response and of CTL activity clearly showed that HIV-1-specific cell-mediated immunity was enhanced by inoculation of the IL-12 expression plasmid. Quantitation of cytokine in the sera of IL-12-inoculated mice revealed that IFN-gamma significantly increased. The enhanced cell-mediated immunity responses were abrogated by combined administration of the IL-12 expression plasmid and neutralizing anti-IFN-gamma Ab. Together, these results suggest that enhanced virus-specific cell-mediated immunity occurred via an endogenously produced IFN-gamma by inoculation of IL-12 expression plasmid.
authors.affiliation: Department of Bacteriology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: K
authors.initials: K
authors.lastname: Hamajima
authors.forename: J
authors.initials: J
authors.lastname: Fukushima
authors.forename: K Q
authors.initials: KQ
authors.lastname: Xin
authors.forename: N
authors.initials: N
authors.lastname: Ishii
authors.forename: I
authors.initials: I
authors.lastname: Aoki
authors.forename: Y
authors.initials: Y
authors.lastname: Ishigatsubo
authors.forename: K
authors.initials: K
authors.lastname: Tani
authors.forename: S
authors.initials: S
authors.lastname: Kawamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Nitta
authors.forename: J
authors.initials: J
authors.lastname: Miyazaki
authors.forename: W C
authors.initials: WC
authors.lastname: Koff
authors.forename: T
authors.initials: T
authors.lastname: Okubo
authors.forename: K
authors.initials: K
authors.lastname: Okuda
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D005260:Female; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007114:Immunization; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D015488:HIV Antigens; D018664:Interleukin-12