Protective action of epidermal growth factor and a fraction from Triticum vulgare extract in mouse tail necrosis.
Several peptide growth factors, including EGF, are known to protect endothelium from oxygen-related damage or ischemia-reperfusion, in vitro experiments show that such protective effect involves endogenous endothelium-related factors like nitric oxide and prostanoids. However, in vivo demonstrations of a possible role in related vascular diseases are lacking. In our experiments, human EGF and fraction C, a 3-10 kDa oligosaccharidic fraction from an aqueous extract of Triticum vulgare, known as growth promoters for several cell types including endothelial cells, were found protective against ischemic necrosis of the mouse tail induced by i.v. k-carrageenin plus endothelin-1. After i.p. injection, peak activities were observed at 10 micrograms/kg EGF and 2 mg/kg fraction C. Pretreatment with L-NAME reduced protection in a dose-dependent manner. Addition of indomethacin increased the effect of L-NAME, suggesting that both nitric oxide and eicosanoids are involved in the protective effect of EGF and fraction C.
Caruso
A
A
Institute of Pharmacology, University of Catania School of Medicine, Italy.
Cutuli
V M
VM
de Bernardis
E
E
Amico-Roxas
M
M
D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000627:therapeutic use*; D007511:Ischemia / Q000150:complications; D008297:Male; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009336:Necrosis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000627:therapeutic use*; D015427:Reperfusion Injury / Q000517:prevention & control; D013623:Tail / Q000098:blood supply / Q000473:pathology*; D014316:Tritium
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014316:Tritium; D004815:Epidermal Growth Factor; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester