Chemical synthesis of Haemophilus influenzae glycopeptide conjugates.
A simple procedure for conjugating synthetic fragments of the capsular polysaccharide of Haemophilus influenzae type b, poly-3-beta-D-ribose-(1,1)-D-ribitol-5-phosphate (sPRP) to linear peptides is described. The procedure consists of (i) reacting the amino group of amino-heptyl sPRP with m-maleimidobenzoyl-N-hydroxysuccinimide (MBS) in phosphate buffer, pH 7.5; (ii) selectively coupling the MBS-modified sPRP to the sulfhydryl group of the cysteine residue of peptides containing functional T-helper cell epitope(s). The glycopeptide conjugates were purified by gel filtration chromatography, biochemically characterized, and elicited protective level of anti-PRP antibody responses in rabbits.
Kandil
A A
AA
Connaught Centre for Biotechnology Research, North York, Ontario, Canada.
Chan
N
N
Klein
M
M
Chong
P
P
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D000939:Epitopes; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000276:immunology*; D018073:Haemophilus Vaccines / Q000737:chemistry / Q000494:pharmacology; D006193:Haemophilus influenzae / Q000737:chemistry*; D007114:Immunization; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000494:pharmacology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D006023:Glycoproteins; D018073:Haemophilus Vaccines; D010446:Peptide Fragments; D011135:Polysaccharides, Bacterial; D014614:Vaccines, Synthetic