title: Internal sclerostomy with a mechanical trephine versus the neodymium:YAG laser in dogs.
abstract: OBJECTIVE
  To experimentally compare and contrast the internal sclerostomy produced using a mechanical trephine with that made by an Nd:YAG laser, and to evaluate the healing patterns, efficacy, and technical advantages of each method.
  
  
  METHODS
  Four healthy dogs with normal eyes were used in this study. The left eye of each dog had an internal sclerostomy performed with an automated mechanical trephine. The right eye of each dog had an internal sclerostomy performed with an Nd:YAG laser. Postoperatively, the intraocular pressure of each eye was measured at regular intervals. The dogs were euthanized successively at 1, 2, 3, and 4 weeks postoperatively, and the morphology of the surgical sites was studied.
  
  
  RESULTS
  The Nd:YAG laser was easier to manipulate and technically less demanding than the mechanical trephine. However, the Nd:YAG laser induced more prominent corneal edema and conjunctival hyperemia compared with the automated trephine. Morphology of the tissue response showed tissue necrosis and greater inflammation with the Nd:YAG laser than with the automated trephine.
  
  
  CONCLUSIONS
  These two techniques demonstrate differences in technical demands and tissue response. Both are characterized by minimal reactive fibrosis of the episcleral and Tenon's capsule fibroblast.
authors.affiliation: Department of Ophthalmology, School of Medicine, University of North Carolina, Chapel Hill 27599-7040, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Peiffer
authors.forename: R D
authors.initials: RD
authors.lastname: Nobles
authors.forename: J
authors.initials: J
authors.lastname: Carter
authors.forename: A D
authors.initials: AD
authors.lastname: Pandya
authors.forename: C
authors.initials: C
authors.lastname: Osborne
authors.forename: S
authors.initials: S
authors.lastname: Freedman
mesh_terms: D000818:Animals; D003228:Conjunctiva / Q000098:blood supply / Q000473:pathology; D003316:Corneal Diseases / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D015715:Corneal Edema / Q000209:etiology / Q000473:pathology; D004285:Dogs; D005260:Female; D005500:Follow-Up Studies; D006940:Hyperemia / Q000209:etiology / Q000473:pathology; D007429:Intraocular Pressure; D053685:Laser Therapy / Q000009:adverse effects / Q000379:methods*; D008297:Male; D011183:Postoperative Complications; D012590:Sclera / Q000473:pathology / Q000601:surgery*; D012599:Sclerostomy / Q000295:instrumentation / Q000379:methods*; D014945:Wound Healing