Heterogeneity of expression of CD68 and other macrophage-associated antigens in human long-term bone marrow culture.
The authors have performed an immunohistochemical study of intact human long-term bone marrow cultures (hLTBMC) grown directly onto glass slides. Between 4 and 12 weeks of growth, such cultures consist of a complex stromal layer supporting foci of haemopoietic cells which undergo granulocytic and monocytic differentiation. As part of a large panel of antibodies employed to characterize monocytes and macrophages within hLTBMC, we included six different anti-CD68 reagents and three antibodies representing a putative new CD group recognizing a macrophage-associated antigen of 130 kDa molecular weight. These gave heterogeneous immunostaining patterns with macrophages and stromal myofibroblastic cells.
Wilkins
B S
BS
University Department of Pathology, Southampton General Hospital, U.K.
Jones
D B
DB
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D015703:Antigens, CD / Q000096:biosynthesis* / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis* / Q000276:immunology; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D000097382:CD68 Molecule
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000939:Epitopes; C067980:CD68 antigen, human; D000097382:CD68 Molecule