Distribution of myomodulin-like and buccalin-like immunoreactivities in the central nervous system and peripheral tissues of the mollusc, Clione limacina.
The distribution of the myomodulin-like and buccalin-like immunoreactivities in the central nervous system and peripheral tissues associated with feeding was examined in the pteropod mollusc Clione limacina by using wholemount immunohistochemical techniques. Immunoreactive neurons and cell clusters were located in all central ganglia except the pleural ganglia, with approximately 50 central neurons reactive to myomodulin antiserum and 60 central neurons reactive to buccalin antiserum. All central ganglia contained a dense network of myomodulin- and buccalin-immunoreactive processes in their neuropil regions and connectives. In the periphery, the primary attention was focused on the tissues associated with feeding, especially feeding structures unique to Clione, such as hook sacs and buccal cones, which are used for prey capture and acquisition. All of these feeding structures contained myomodulin-immunoreactive and buccalin-immunoreactive fibers, with each peptide family showing specific innervation fields that were common in buccal cones and were totally different in the hook sacs. The specific central and peripheral distribution of myomodulin-like and buccalin-like immunoreactivities as well as specific effects of the exogenous peptides on identified neurons involved in the control of feeding behavior and swimming suggest that neuropeptides from myomodulin and buccalin families act as neurotransmitters or neuromodulators in a variety of central circuits and in the peripheral neuromuscular systems associated with feeding in Clione limacina.
Norekian
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TP
Department of Zoology, Arizona State University, Tempe 85282-1501, USA. Attpn@asuvm.inre.asu.edu
Satterlie
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RA
D000818:Animals; D000918:Antibody Specificity; D004594:Electrophysiology; D005247:Feeding Behavior / Q000502:physiology; D017952:Ganglia, Invertebrate / Q000737:chemistry; D007150:Immunohistochemistry; D008974:Mollusca / Q000502:physiology*; D009055:Mouth / Q000502:physiology; D009420:Nervous System / Q000737:chemistry; D009479:Neuropeptides / Q000032:analysis / Q000276:immunology*; D013550:Swimming / Q000502:physiology; D014921:Wings, Animal / Q000502:physiology
D009479:Neuropeptides; C053302:myomodulin; C058692:buccalin