Discovery of disseminated J96-like strains of uropathogenic Escherichia coli O4:H5 containing genes for both PapG(J96) (class I) and PrsG(J96) (class III) Gal(alpha1-4)Gal-binding adhesins.
The pyelonephritis-associated adhesin gene papG of Escherichia coli occurs in three variants. Whereas the class II and class III variants are common among human urinary tract infection isolates, the class I allele, despite being the first cloned, has previously been found only in source strain J96. Five strains have been discovered from geographically diverse locales that, like J96, contain both the class I and class III papG alleles. One strain caused bacteremia, whereas 4 caused cystitis. Like J96, all 5 had group III capsule genes, expressed the H5 flagellar antigen and the F13 fimbrial antigen, and exhibited similar genomic patterns and virulence factor profiles. These findings demonstrate that the class I papG allele is not unique to J96 but is present in a group of extraintestinal isolates of E. coli O4:H5 that represent a disseminated virulent clonal group.
Johnson
J R
JR
Department of Medicine, University of Minnesota, Minneapolis, USA.
Russo
T A
TA
Scheutz
F
F
Brown
J J
JJ
Zhang
L
L
Palin
K
K
Rode
C
C
Bloch
C
C
Marrs
C F
CF
Foxman
B
B
D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000483:Alleles; D015139:Blotting, Southern; D004187:Disaccharides / Q000378:metabolism*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D006801:Humans; D012703:Serotyping; D014552:Urinary Tract Infections / Q000382:microbiology*; D014774:Virulence
D018830:Adhesins, Escherichia coli; D004187:Disaccharides; C092426:PapG protein, E coli; C055075:galactosyl-(1-3)galactose; D036781:Fimbriae Proteins