Copurification of ribosomal protein S2 and DNA-dependent RNA polymerase from heat-shocked cells of Bacillus subtilis.
The DNA-dependent RNA polymerases from heat-shocked and vegetatively grown cells of Bacillus subtilis were isolated and compared. The RNA polymerase from non-stressed cells had the well known alpha, beta, beta' and sigma composition of eubacterial RNA polymerases. The RNA polymerase from heat-shocked cells exhibited one additional band shown by SDS-PAGE. N-terminal sequencing of the first 16 amino acids of the associated protein demonstrated its identity with the ribosomal protein S2.
Büttner
K
K
Ernst-Moritz-Arndt-Universität, Institut für Mikro- und Molekularbiologie, Greifswald, Germany.
Pich
A
A
Neubauer
P
P
Schmid
R
R
Bahl
H
H
Hecker
M
M
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry* / Q000254:growth & development; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D018869:Heat-Shock Response; D012269:Ribosomal Proteins / Q000032:analysis / Q000302:isolation & purification*; D017421:Sequence Analysis
D012269:Ribosomal Proteins; C030004:ribosomal protein S2; D012321:DNA-Directed RNA Polymerases