title: In vivo packaging of bacteriophage lambda monomeric chromosomes.
abstract: There is an apparent paradox between the reported requirements for lambda DNA packaging in vivo and in vitro. In vivo, DNA concatemers are required for packaging. On the other hand, in vitro, packaging extracts can encapsidate either linear or circular monomeric lambda DNA. Perhaps cellular nucleases restrict the in vivo ability of monomers to package by degrading a free double chain end present as an intermediate in the packaging reaction. Consistent with this hypothesis, enhanced packaging of monomers was found in an ExoV- host. No additional enhancement was noted in a host also mutant for sbcB and sbcC. We isolated a mutant phage for which in vivo packaging of monomeric lambda chromosomes is increased about 10(3)-fold. The responsible mutation (plm1 for packages lambda monomers) was mapped to cro, sequenced, and found to cause a change from Ala29 to Ser in the alpha3 helix of Cro's DNA binding domain. Density transfer experiments showed that packaging of both plm1 and wild-type lambda was aided by allowing some DNA synthesis. However, the packaged chromosomes had not themselves undergone a full round of replication and therefore were not part of a canonical concatemer made by replication. Other tests showed that packaged phage had not been part of concatemers made by recombination or by annealing at cos. Our results with wild-type lambda also favor models in which two cos sites are needed for packaging, but these sites need not be in cis. In lambda plm1, replication intermediates may serve as substrates for encapsidation.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA.
authors.forename: L C
authors.initials: LC
authors.lastname: Thomason
authors.forename: D S
authors.initials: DS
authors.lastname: Thaler
authors.forename: M M
authors.initials: MM
authors.lastname: Stahl
authors.forename: F W
authors.initials: FW
authors.lastname: Stahl
mesh_terms: D010582:Bacteriophage lambda / Q000502:physiology*; D003360:Cosmids; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000378:metabolism; D009154:Mutation; D011938:Rec A Recombinases / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D019065:Virus Assembly
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D011938:Rec A Recombinases; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C030894:terminase; C028450:exodeoxyribonuclease I