title: HLA-A locus DNA typing of the 4AOH cell panel by arms PCR.
abstract: As part of the Fourth Asia-Oceania Histocompatibility (4AOH) Workshop, the authors have demonstrated a method of DNA-based tissue typing of the HLA-A locus using ARMS-designed primers in a panel of specific PCR reactions. The study was carried out blind under Workshop conditions and the results confirm the method as an accurate means of determining HLA-A locus tissue types.
authors.affiliation: Imperial Cancer Research Fund, Institute of Molecular, Medicine, John Radcliffe Hospital, Oxford, UK.
authors.forename: P
authors.initials: P
authors.lastname: Krausa
authors.forename: M J
authors.initials: MJ
authors.lastname: Browning
authors.forename: J H
authors.initials: JH
authors.lastname: Moses
authors.forename: J G
authors.initials: JG
authors.lastname: Bodmer
mesh_terms: D002874:Chromosome Mapping; D004247:DNA / Q000145:classification; D005240:Feasibility Studies; D015234:HLA-A Antigens / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D015234:HLA-A Antigens; D004247:DNA