Clarification of the promoter structure of the osmoregulated gpd1+ gene encoding an isozyme of NADH-dependent glycerol-3-phosphate dehydrogenase in fission yeast.
The gpd1+ gene of Schizosaccharomyces pombe, encoding an isozyme of NADH-dependent glycerol-3-phosphate dehydrogenase, is responsible for an osmoinducible glycerol production in response to external high osmotic stimuli, and its expression is osmoregulated at the level of transcription. In this study, the structure of the osmoinducible promoter of gpd1+ is described. Not only the core promoter elements including the putative TATA-box and transcription start site, but also a short upstream activation sequence (UAS) was identified, which are involved in the osmotic induction of the gpd1+ gene through the Wis1 MAP (mitogen activated protein) kinase pathway.
Ohmiya
R
R
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
Aiba
H
H
Yamada
H
H
Mizuno
T
T
D001483:Base Sequence; D015152:Blotting, Northern; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D009994:Osmolar Concentration; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics; D016385:TATA Box; D014158:Transcription, Genetic / Q000235:genetics
D007527:Isoenzymes; D009243:NAD; D005993:Glycerolphosphate Dehydrogenase; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005990:Glycerol