title: Copurification of ribosomal protein S2 and DNA-dependent RNA polymerase from heat-shocked cells of Bacillus subtilis.
abstract: The DNA-dependent RNA polymerases from heat-shocked and vegetatively grown cells of Bacillus subtilis were isolated and compared. The RNA polymerase from non-stressed cells had the well known alpha, beta, beta' and sigma composition of eubacterial RNA polymerases. The RNA polymerase from heat-shocked cells exhibited one additional band shown by SDS-PAGE. N-terminal sequencing of the first 16 amino acids of the associated protein demonstrated its identity with the ribosomal protein S2.
authors.affiliation: Ernst-Moritz-Arndt-Universität, Institut für Mikro- und Molekularbiologie, Greifswald, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Büttner
authors.forename: A
authors.initials: A
authors.lastname: Pich
authors.forename: P
authors.initials: P
authors.lastname: Neubauer
authors.forename: R
authors.initials: R
authors.lastname: Schmid
authors.forename: H
authors.initials: H
authors.lastname: Bahl
authors.forename: M
authors.initials: M
authors.lastname: Hecker
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry* / Q000254:growth & development; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D018869:Heat-Shock Response; D012269:Ribosomal Proteins / Q000032:analysis / Q000302:isolation & purification*; D017421:Sequence Analysis
chemical_list: D012269:Ribosomal Proteins; C030004:ribosomal protein S2; D012321:DNA-Directed RNA Polymerases