Solubilizing effects caused by the nonionic surfactant dodecylmaltoside in phosphatidylcholine liposomes.
The interaction of the nonionic surfactant dodecylmaltoside (DM) with phosphatidylcholine liposomes was investigated. Permeability alterations were detected as a change in 5(6)-carboxyfluorescein released from the interior of vesicles and bilayer solubilization as a decrease in the static light scattered by liposome suspensions. This surfactant showed higher capacity to saturate and solubilize PC liposomes and greater affinity with these structures than those reported for the octyl glucoside. At subsolubilizing level an initial maximum in the bilayer/water partitioning (K) followed by an abrupt decrease of this parameter occurred as the effective molar ratio of surfactant to phospholipid in bilayers (Re) rose. However, at solubilizing level a direct dependence was established between both parameters. A direct correlation took place in the initial interaction steps (Re up to 0.28) between the growth of vesicles, their fluidity, and Re. A similar direct dependence was established during solubilization (Re range from 0.9 to 1.7) between the decrease in both the surfactant-PC aggregate size, the light scattering of the system, and Re (composition of aggregates). The fact that the free DM concentration at subsolubilizing and solubilizing levels showed values lower than and similar to its critical micelle concentration indicates that permeability alterations and solubilization were determined, respectively, by the action of surfactant monomer and by the formation of mixed micelles.
de la Maza
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A
Departamento de Tensioactivos, Centro de Investigación y Desarrollo, Consejo Superíor de Investigaciones Científicas, Barcelona, Spain.
Parra
J L
JL
D005452:Fluoresceins; D005960:Glucosides / Q000494:pharmacology*; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity / Q000187:drug effects; D010316:Particle Size; D010539:Permeability / Q000187:drug effects; D010713:Phosphatidylcholines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D012044:Regression Analysis; D012542:Scattering, Radiation; D012995:Solubility / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology*
D005452:Fluoresceins; D005960:Glucosides; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D013501:Surface-Active Agents; C024098:6-carboxyfluorescein; C040358:dodecyl maltoside