title: Age-related reciprocal modulation of interleukin-1beta and interleukin-1 receptors in the mouse brain-endocrine-immune axis.
abstract: The cytokine interleukin-1 (IL-1) alters a variety of immune, central nervous system and neuroendocrine activities characteristic of an integrator of the brain-endocrine-immune response to stress. In an attempt to define the regulation of IL-1 and IL-1 receptors in the mouse brain-endocrine-immune axis during maturation, we measured tissue levels of IL-1beta using an ELISA and iodine-125-labeled recombinant human interleukin-1alpha ([125I]IL-1alpha) binding in hippocampus, pituitary, testis and spleen following intraperitoneal injection of saline or the bacterial endotoxin, lipopolysaccharide (LPS) in 3- and 24-week-old C57BL/6 mice. Basal IL-1beta levels were detectable in all the tissues examined. Basal levels of IL-1beta in the hippocampus, spleen and testis in 24-week-old mice were significantly higher than those in 3-week-old mice. [125I]IL-1alpha binding was detectable in all the mouse tissues examined and [125I]IL-1alpha binding levels in the pituitary, spleen and testis in 3-week-old mice were significantly higher than those in 24-week-old mice. To further evaluate the modulation of IL-1 receptors in aging, we measured [125I]IL-1alpha binding in 2-, 5-, 10-, 18-, and 24-month-old mice. [125I]IL-1alpha binding in testis in 24-month-old mice was higher than the other groups; [125I]IL-1alpha binding in the hippocampus and spleen was unchanged during these periods. Dramatic increases in IL-1beta concentrations were observed at 2 h after LPS injection in the pituitary, spleen, testis and plasma in both 3- and 24-week-old mice groups. In contrast, IL-1beta levels in the hippocampus increased in response to LPS injection only in 24-week-old mice. [125I]IL-1alpha binding in hippocampus was significantly decreased in 24- but not in 3-week-old mice after LPS injection. [125I]IL-1alpha binding in the spleen and testis were significantly decreased in both age groups following LPS administration. These data demonstrate differential regulation of IL-1 and its receptors in the brain-endocrine-immune axis during maturation and in response to endotoxin challenge.
authors.affiliation: Second Department of Internal Medicine, Kochi Medical School, Nankoku, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takao
authors.forename: I
authors.initials: I
authors.lastname: Nagano
authors.forename: C
authors.initials: C
authors.lastname: Tojo
authors.forename: T
authors.initials: T
authors.lastname: Takemura
authors.forename: S
authors.initials: S
authors.lastname: Makino
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: E B
authors.initials: EB
authors.lastname: De Souza
mesh_terms: D000367:Age Factors; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010902:Pituitary Gland / Q000378:metabolism*; D017472:Receptors, Interleukin-1 / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D017472:Receptors, Interleukin-1