Study of apoptosis in human lymphocytes by toxic substances implicated in toxic oil syndrome.
Toxic Oil Syndrome is a multisystemic disease that occurred in epidemic proportions in Spain in 1981 caused by the ingestion of rapeseed oil denatured with aniline. Several data implicate T cells in the pathogenesis of the disease. We evaluated the mechanisms of cytotoxicity in human lymphocytes of TOS-related products: aniline, 3-(N-phenylamino)-1,2-propanediol and its mono- and di-oleyl esters and eosinophilia myalgia-related product such as 3-(phenylamino)-L-alanine, which is chemically similar to 3-(N-phenylamino)-1,2-propanediol, and has been found in manufactured L-tryptophan. Our results show that only di-oleyl ester of 3-(N-phenylamino)-1,2-propanediol induces apoptosis in human lymphocytes, in a concentration and time-dependent way, confirmed by morphology, expression of phosphatidylserine in membrane and analysis of DNA degradation.
Gallardo
S
S
Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain.
Cárdaba
B
B
del Pozo
V
V
de Andrés
B
B
Cortegano
I
I
Jurado
A
A
Tramón
P
P
Palomino
P
P
Lahoz
C
C
D000409:Alanine / Q000031:analogs & derivatives* / Q000633:toxicity; D000814:Aniline Compounds / Q000633:toxicity*; D017209:Apoptosis; D001937:Brassica; D002478:Cells, Cultured; D004396:Coloring Agents; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005229:Fatty Acids, Monounsaturated; D005434:Flow Cytometry; D005517:Foodborne Diseases / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D010938:Plant Oils / Q000506:poisoning; D011409:Propylene Glycols / Q000633:toxicity*; D000074262:Rapeseed Oil; D013577:Syndrome; D014304:Triolein / Q000633:toxicity; D014343:Trypan Blue
D000814:Aniline Compounds; D004396:Coloring Agents; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D011409:Propylene Glycols; D000074262:Rapeseed Oil; C092834:3-(N-phenylamino)-1,2-propanediol 1-oleoyl ester; D014304:Triolein; C078798:3-(phenylamino)alanine; C077151:3-phenylamino-1,2-propanediol; D004247:DNA; C092835:3-(N-phenylamino)-1,2-propanediol 1,2-dioleoyl ester; D014343:Trypan Blue; D000409:Alanine; C023650:aniline