title: RIC1, a novel gene required for ribosome synthesis in Saccharomyces cerevisiae.
abstract: We isolated a temperature-sensitive mutant of Saccharomyces cerevisiae in which transcription both of ribosomal protein genes and of ribosomal RNA is defective at the non-permissive temperature. Temperature-sensitivity for growth is recessive and segregates 2:2. The wild type gene, termed RIC1 (for ribosome control) was cloned by complementation of the temperature-sensitive phenotype from a genomic DNA library based on the CEN plasmid. RIC1 encodes a protein of 1056 amino acid (aa) residues including a putative nuclear localization sequence. Data base searches revealed that RIC1 is a novel gene and predicted aa sequence share some sequence similarity with viral transcriptional regulatory proteins.
authors.affiliation: Department of Biochemistry and Biophysics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Minami-ku, Japan. kmizuta@ipc.hiroshima-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Mizuta
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: M
authors.initials: M
authors.lastname: Sugiyama
authors.forename: M
authors.initials: M
authors.lastname: Nishiyama
authors.forename: J R
authors.initials: JR
authors.lastname: Warner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins / Q000235:genetics; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D043324:beta-Fructofuranosidase
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D008565:Membrane Proteins; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; C105592:RIC1 protein, S cerevisiae; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase