title: Study on serum-free media for genetically engineered CHO cells producing prourokinase.
abstract: Based on the initial optimization of DMEM:F12(1:1), two kinds of serum-free media, 11G-SG-SFM and 11G-SE-SFM, were formulated by using orthogonal experiments for the growth of genetically engineered CHO cell line 11G and the production of recombinant prourokinase (pro-UK) in a suspension culture, respectively. Growth of 11G cells in 11G-SG-SFM was comparable with that observed in DMEM:F12 + 5% NBS and CHO-S-SFM. 11G cells grew slowly in 11G-SE-SFM, but secreted a relatively high level of pro-UK. The pro-UK productivity of 11G cells in 11G cells in 11G-SE-SFM reached 800 to 1,000 IU/10(6). d-1 and was about 80% and 20% higher than in DMEM:F12 + 5% NBS and CHO-S-SFM, respectively.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.forename: X
authors.initials: X
authors.lastname: Jia
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Xiao
mesh_terms: D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D006224:Cricetinae; D016895:Culture Media, Serum-Free; D005818:Genetic Engineering; D011498:Protein Precursors / Q000096:biosynthesis; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D016895:Culture Media, Serum-Free; D011498:Protein Precursors; D014568:Urokinase-Type Plasminogen Activator