title: The first immunoglobulin-like neural cell adhesion molecule (NCAM) domain is involved in double-reciprocal interaction with the second immunoglobulin-like NCAM domain and in heparin binding.
abstract: To study the function of the first immunoglobulin (Ig)-like domain of the neural cell adhesion molecule (NCAM), it was produced as a recombinant fusion protein in a bacterial expression system and as a recombinant protein in a eukaryotic expression system of the yeast Pichia pastoris. For comparison, other NCAM domains were also produced as fusion proteins. By means of surface plasmon resonance analysis, it was shown that the first Ig-like NCAM domain binds the second Ig-like NCAM domain with a dissociation constant 5.5 +/- 1.6 x 10(-5) M. Furthermore, it was found that the first Ig-like domain binds heparin. It was also demonstrated that the second Ig-like NCAM domain binds heparin and that both domains bind collagen type I via heparin but not collagen type I directly.
authors.affiliation: Protein Laboratory, Panum Institute, University of Copenhagen, Blegdamsvej 3C, Building 6.2, DK-2200 Copenhagen, Denmark.
authors.forename: V V
authors.initials: VV
authors.lastname: Kiselyov
authors.forename: V
authors.initials: V
authors.lastname: Berezin
authors.forename: T E
authors.initials: TE
authors.lastname: Maar
authors.forename: V
authors.initials: V
authors.lastname: Soroka
authors.forename: K
authors.initials: K
authors.lastname: Edvardsen
authors.forename: A
authors.initials: A
authors.lastname: Schousboe
authors.forename: E
authors.initials: E
authors.lastname: Bock
mesh_terms: D000818:Animals; D001665:Binding Sites; D002449:Cell Aggregation / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D006493:Heparin / Q000378:metabolism*; D007136:Immunoglobulins / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism; D009474:Neurons / Q000166:cytology; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D007136:Immunoglobulins; D019006:Neural Cell Adhesion Molecules; D011993:Recombinant Fusion Proteins; D006493:Heparin