The effect of exogenous gangliosides on matrix metalloproteinase secretion by human glioma cells in vitro.
Matrix metalloproteinases (MMPs) are zinc-dependent peptidases and are amongst those enzymes responsible for extracellular matrix (ECM) degradation during tumour-cell migration. Gangliosides are a family of acidic membrane glycolipids thought to play a role during cell development, differentiation and oncogenic transformation. In this descriptive study, we investigated the effects of six exogenous gangliosides (GM1, GM3, GD1a, GD1b, GD3 and GT1b) on the secretion of MMP-2 (72 kDa gelatinase or gelatinase-A) and MMP-9 (92 kDa gelatinase or gelatinase-B). Cell-conditioned media from eight human glioma-derived cell-lines served as the source of MMPs and were investigated using SDS-PAGE zymography. Six of the cell lines showed upregulation of secretion of both enzymes by all six gangliosides. Of the remaining two cell lines, one showed inhibition of MMP secretion by all gangliosides and the other had a small but differential response to the range of gangliosides investigated. These results suggest that gangliosides may stimulate glioma cell invasiveness by promoting MMP expression.
Maidment
S L
SL
Department of Neuropathology, Institute of Psychiatry, Denmark Hill, London, U.K.
Merzak
A
A
Koochekpour
S
S
Rooprai
H K
HK
Rucklidge
G J
GJ
Pilkington
G J
GJ
D017364:Collagenases / Q000378:metabolism*; D017077:Culture Media, Conditioned; D004591:Electrophoresis, Polyacrylamide Gel; D005732:Gangliosides / Q000494:pharmacology*; D018093:Gelatinases / Q000378:metabolism*; D005910:Glioma / Q000201:enzymology*; D006801:Humans; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D017077:Culture Media, Conditioned; D005732:Gangliosides; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9