title: Protein B23 is an important human factor for the nucleolar localization of the human immunodeficiency virus protein Tat.
abstract: Nucleolar shuttle protein B23 was found to bind to human immunodeficiency virus protein Tat, and this binding required the nucleolar localization motif of Tat. A fusion protein containing the B23 binding domain and beta-galactosidase caused mislocalization of Tat to the cytoplasm and inhibited the transactivation activity of Tat. These data suggest that B23 is a human factor necessary for the nucleolar localization of Tat.
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA. yiping@tmc.bcm.edu
authors.forename: Y P
authors.initials: YP
authors.lastname: Li
mesh_terms: D001665:Binding Sites; D002466:Cell Nucleolus / Q000378:metabolism*; D015696:Gene Products, tat / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006678:HIV / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000502:physiology*; D000090243:Nucleophosmin; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D001616:beta-Galactosidase / Q000494:pharmacology; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D015696:Gene Products, tat; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D000090243:Nucleophosmin; D001616:beta-Galactosidase