title: Use of nested polymerase chain reaction to identify feline herpesvirus in ocular tissue from clinically normal cats and cats with corneal sequestra or conjunctivitis.
abstract: OBJECTIVE
  To use a nested polymerase chain reaction (PCR) to detect feline herpesvirus (FHV-1) DNA in conjunctiva or cornea from clinically normal cats and cats with conjunctivitis or corneal sequestra.
  
  
  METHODS
  Conjunctival snip biopsy specimens from 50 cats with conjunctivitis and 50 clinically normal cats; 28 keratectomy specimens from 26 cats with sequestra, and 13 specimens from clinically normal cats.
  
  
  METHODS
  Tissue specimens were digested, and FHV-1 DNA was amplified, using a double round of PCR. Products were visualized by use of agarose gel electrophoresis.
  
  
  RESULTS
  Polymerase chain reaction was positive in 27 of 50 (54%) conjunctival specimens from cats with conjunctivitis and 6 of 50 (12%) specimens from clinically normal cats. Difference in the results between cats with conjunctivitis and clinically normal cats was statistically significant. Polymerase chain reaction was positive in 5 of 28 (18%) corneal specimens from cats with sequestra and 6 of 13 (46%) clinically normal cats. Distribution of positive results between clinically normal cats and those with sequestra was not significant.
  
  
  CONCLUSIONS
  Cats with conjunctivitis were more likely to have a positive PCR result than were clinically normal cats, making it likely that FHV-1 was associated with the disease state. Herpesvirus DNA could not be detected in most corneas from cats with sequestra.
  
  
  CONCLUSIONS
  Polymerase chain reaction is a useful clinical test for identifying FHV-1 DNA in cats with conjunctivitis, yielding greater sensitivity over that of current available tests. Herpesvirus may be less of a cause of corneal sequestration in the commonly affected breeds, Himalayan and Persian, than other factors, such as lagophthalmos or corneal metabolic defects.
authors.affiliation: Department of Small Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
authors.forename: J
authors.initials: J
authors.lastname: Stiles
authors.forename: M
authors.initials: M
authors.lastname: McDermott
authors.forename: D
authors.initials: D
authors.lastname: Bigsby
authors.forename: M
authors.initials: M
authors.lastname: Willis
authors.forename: C
authors.initials: C
authors.lastname: Martin
authors.forename: W
authors.initials: W
authors.lastname: Roberts
authors.forename: C
authors.initials: C
authors.lastname: Greene
mesh_terms: D000208:Acute Disease; D000818:Animals; D001706:Biopsy / Q000379:methods / Q000662:veterinary; D002371:Cat Diseases / Q000175:diagnosis* / Q000473:pathology; D002415:Cats; D002417:Cattle; D002908:Chronic Disease; D003228:Conjunctiva / Q000737:chemistry / Q000473:pathology / Q000821:virology*; D003231:Conjunctivitis / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D003315:Cornea / Q000737:chemistry / Q000473:pathology / Q000821:virology*; D003316:Corneal Diseases / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary; D005260:Female; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary
chemical_list: D004279:DNA, Viral