Selective cell ablation in transgenic mice expression E. coli nitroreductase.
The gene encoding E. coli nitroreductase (NTR) was expressed in the luminal cells of the mammary gland of transgenic mice using the ovine beta-lactoglobulin promoter. Treatment of NTR expressing animals with the prodrug CB1954 (5-aziridin-1-yl-2-4-dinitrobenzamide) resulted in a rapid and selective killing of this population of cells whereas the closely associated myoepithelial cells were unaffected. NTR-mediated inducible cell ablation offers a number of advantages over the use of HSV1-tk for the selective killing of cells in vivo.
Clark
A J
AJ
Division of Molecular Biology, Roslin Institute, Roslin, UK.
Iwobi
M
M
Cui
W
W
Crompton
M
M
Harold
G
G
Hobbs
S
S
Kamalati
T
T
Knox
R
R
Neil
C
C
Yull
F
F
Gusterson
B
B
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001388:Aziridines / Q000494:pharmacology*; D018929:Cell Culture Techniques; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005260:Female; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D007124:Immunoenzyme Techniques; D007774:Lactation; D007782:Lactoglobulins / Q000235:genetics; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008822:Mice, Transgenic; D009601:Nitroreductases / Q000235:genetics* / Q000378:metabolism; D011355:Prodrugs / Q000494:pharmacology*
D000970:Antineoplastic Agents; D001388:Aziridines; D007782:Lactoglobulins; D011355:Prodrugs; C100099:tretazicar; D009601:Nitroreductases