title: In vitro culture of Taenia crassiceps larval cells and cyst regeneration after injection into mice.
abstract: Taenia crassiceps cysticerci were disrupted through trypsinization to isolate cells which can be maintained in culture for up to 15 days. When injected intraperitoneally into susceptible BALB/cAnN mice, complete cysticerci were recovered in a number that is proportional to the quantity of injected cells. Thus, cysticerci contain cells which can reconstitute complete cysts, suggesting that individual cells play a role, independent to budding, during asexual multiplication of T. crassiceps cysticerci in the peritoneal cavity of mice. In contrast, injection of the cells into resistant C57BL/6J mice does not result in the recovery of complete cysts. These findings provide a new experimental model to identify resistance factors in the hosts, for the in vitro screening of anti-cysticerci drugs and for the genetic manipulation of cysticerci through recombinant DNA techniques.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F., México.
authors.forename: A
authors.initials: A
authors.lastname: Toledo
authors.forename: C
authors.initials: C
authors.lastname: Cruz
authors.forename: G
authors.initials: G
authors.lastname: Fragoso
authors.forename: J P
authors.initials: JP
authors.lastname: Laclette
authors.forename: M T
authors.initials: MT
authors.lastname: Merchant
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: E
authors.initials: E
authors.lastname: Sciutto
mesh_terms: D000818:Animals; D003470:Culture Media; D003551:Cysticercosis / Q000469:parasitology*; D003552:Cysticercus / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D003470:Culture Media