Determination of okadaic acid by micellar electrokinetic chromatography with ultraviolet detection.
Micellar electrokinetic chromatography (MEKC) with ultraviolet (UV) detection was applied for the determination of non-derivatized phycotoxins associated with diarrhoetic shellfish poisoning. A detection limit for 40 pg of okadaic acid (OA) was achieved. The UV intensities of this toxin measured at 200 nm showed good linearity in the range 40-640 pg. OA was detected in mussels spiked with 10 ng/g whole tissue. The presence of OA and dinophysistoxin-2 was observed in the crude extract of the dinoflagellate Prorocentrum lima.
Bouaïcha
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N
CEMATMA, Laboratoire de Chimie Analytique, ESPCI, Paris, France.
Hennion
M C
MC
Sandra
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P
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002845:Chromatography / Q000379:methods*; D004586:Electrophoresis; D008387:Marine Toxins / Q000737:chemistry; D019319:Okadaic Acid / Q000302:isolation & purification*; D010839:Phytoplankton / Q000737:chemistry; D014466:Ultraviolet Rays
D008387:Marine Toxins; D019319:Okadaic Acid