Genomic MHC haplotyping of mutant cell lines using a novel marker.
The aim of this study was to characterize MHC mutant cell lines by studying haplospecific markers within the MHC and specifically in the 250 kilobase (kb) region between the HLA B and TNF loci. This region has been difficult to define because of the lack of appropriate markers. Spontaneous MHC mutants were isolated after immunoselection with an anti-HLA A2 monoclonal antibody and complement. Ten mutants were characterized using serological or allelic and genomic DNA markers within the HLA A to HLA DQ region of the MHC. Most mutants lost at least the 3 megabases of DNA from HLA A to HLA DQ viz the whole haplotype carrying HLA A2. Variants which have lost either HlA A alone or HLA A and HLA B were also found. The results show that it is possible to map the extent of the deletion between HLA B and TNF. Haplospecific scanning patterns for the CL region appear particularly useful.
Degli-Esposti
M A
MA
Department of Clinical Immunology, Royal Perth Hospital, Western Australia.
Williamson
J F
JF
Tay
G K
GK
Salmon
B
B
Joseph
G A
GA
Morley
A A
AA
Dawkins
R L
RL
D002460:Cell Line; D017353:Gene Deletion; D005819:Genetic Markers; D015894:Genome, Human; D006680:HLA Antigens / Q000235:genetics; D006239:Haplotypes; D006650:Histocompatibility Testing; D006801:Humans; D008285:Major Histocompatibility Complex; D005810:Multigene Family; D009154:Mutation; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D005819:Genetic Markers; D006680:HLA Antigens; D014409:Tumor Necrosis Factor-alpha