title: Comparison of cytotoxicity of man-made vitreous fibres.
abstract: The purpose of the study was to compare the cytotoxicity of man-made vitreous fibres (MMVFs): four refractory ceramic fibres (RCFs 1-4), two glasswool fibres (MMVF 10 and 11), a rockwool fibre (MMVF 21) and a slagwool fibre (MMVF 22). The ability of the fibres to induce haemolysis in sheep erythrocytes, to release lactate dehydrogenase (LDH) from rat alveolar macrophages (AM) and to increase the production of reactive oxygen metabolites (ROMs) in human polymorphonuclear leukocytes (PML) was studied. To assess the relative cytotoxicity of MMVFs, their toxicity was compared with that induced by quartz, chrysotile or titanium dioxide. MMVFs induced a modest, but dose-dependent, increase of haemolysis at doses of 0.5, 2.5 and 5.0 mg ml-1. The amount of haemolysis and LDH release induced by MMVFs was generally similar to that induced by titanium dioxide. Glasswool fibre MMVF 10 induced less LDH release from rat AM than rockwool MMVF 21 or slagwool MMVF 22 fibres, whereas glasswool fibre MMVF 11 induced less LDH release than slagwool fibre MMVF 22 (P < 0.05). All fibres also dose-dependently increased the production of ROMs at doses between 25 and 500 micrograms ml-1. The shapes of the time-courses of MMVF-induced production of ROMs suggest that the mechanisms whereby the different fibres induce ROM production may exhibit similar features. There are clear-cut differences in the potency of various MMVFs to induce cytotoxicity and oxidative burst. The present results also emphasize the importance of using several measures of toxicity when assessing the biological activity of various fibres in vitro.
authors.affiliation: National Public Health Institute, Division of Environmental Health, Kuopio, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Luoto
authors.forename: M
authors.initials: M
authors.lastname: Holopainen
authors.forename: M
authors.initials: M
authors.lastname: Sarataho
authors.forename: K
authors.initials: K
authors.lastname: Savolainen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017632:Asbestos, Serpentine / Q000633:toxicity; D002516:Ceramics / Q000633:toxicity*; D003252:Construction Materials / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects*; D005898:Glass; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D019016:Mineral Fibers / Q000633:toxicity; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011791:Quartz / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012756:Sheep; D014025:Titanium / Q000633:toxicity
chemical_list: D017632:Asbestos, Serpentine; D019016:Mineral Fibers; D017382:Reactive Oxygen Species; D011791:Quartz; C009495:titanium dioxide; D014025:Titanium; D007770:L-Lactate Dehydrogenase