[Detection of clonality of cutaneous T-cell lymphomas using polymerase chain reaction].
Differentiation of a cutaneous lymphoma from a reactive lymphoid infiltrate is a demanding challenge for the pathologist. In this retrospective study we examined 24 paraffin-embedded tissue samples from lesions diagnosed as lymphomas and 7 control samples of skin affected by benign changes and with pronounced lymphoid infiltrates for clonal rearrangement of the gamma T-cell-receptor. Using PCR technology we demonstrated clonality in 22 cases of lymphoma (92%). Thus, the primer combination used in this study covering the four main groups (I-IV) of the variable region of the gamma T-cell receptor gene allows high sensitivity. No clonality was demonstrable in any of the 7 control cases. This study demonstrates the growing importance of PCR technology for the diagnosis of lymphoma.
Graf
A
A
Dermatologische Klinik und Poliklinik, Ludwig-Maximilians-Universität, München.
Kaudewitz
P
P
Simon
M
M
Kind
P
P
Sander
C A
CA
D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / Q000235:genetics; D006801:Humans; D016410:Lymphoma, T-Cell, Cutaneous / Q000235:genetics* / Q000473:pathology; D009182:Mycosis Fungoides / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology
Nachweis von Klonalität in kutanen T-Zellymphomen mittels der Polymerasekettenreaktion.