Time-lapse changes of d- and l-enantiomers of racemic (dl)-ethylamphetamine in human urine.
The time-lapse changes of d- and i-forms in urine specimens collected in the 24 h after the oral dosing of two adult male subjects with 20 mg of racemic (dl)-ethylamphetamine (EAMP)-HCl were examined by high-performance liquid chromatography. The percentage of the excreted dose of nonmetabolized l-EAMP was larger than that of d-EAMP, and the percentage of the excreted dose of metabolized l-amphetamine (AMP) was smaller than that of d-AMP in both subjects, A and B. These differences were observed 4.5-24 h after administration of the drug. The changes in the l/d ratio of EAMP and AMP were not similar between subjects A and B, but the change in the total l/d ratio was nearly the same. The regression line for subject A was as follows: y = 0.021323x + 0.98399. The regression line for subject B was as follows: y = 0.020947x + 0.94893. These two regression lines suggested that the time lapsed after the administration to humans could be predicted. The total percent of the excreted doses of EAMP and AMP was 47.46% (the d-forms, 20.73%; the l-forms, 26.73%) for subject A and was 31.43% (the d-forms, 14.14%; the l-forms, 17.29%) for subject B. The l/d ratio was 1.29 for subject A and 1.22 for subject B, which was somewhat higher than that (1.01) of the dl-EAMP-HCl used.
Nagai
T
T
Department of Legal Medicine, Dokkyo University School of Medicine, Tochigi, Japan.
Kanaya
H
H
Matsushima
K
K
Kamiyama
S
S
D000662:Amphetamines / Q000652:urine*; D003913:Dextroamphetamine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008297:Male; D013237:Stereoisomerism; D013997:Time Factors
D000662:Amphetamines; C016434:ethylamphetamine; C029116:d-N-ethylamphetamine; D003913:Dextroamphetamine