[Involvement of enhanced glutamate release in halogenated volatile anesthetic-induced excitation in mouse brain].
The present experiments aimed to elucidate the mechanisms of excitatory effects of volatile anesthetics. In ddN mice, enflurane (2% in air), sevoflurane (2%) or isoflurane (1.2%) induced opisthotonus. In ddY mice, a halogenated ethane, tetrachlorodifluoroethane (CCl2FCCl2F:DF-112, 2%) induced tonic-clonic convulsion. These CNS excitatory effects were suppressed by pretreatment with an N-methyl-D-aspartate antagonist, MK-801 (0.5mg.kg-1). Halogenated volatile anesthetics (enflurane, sevoflurane, isoflurane and halothane) increased glutamate release from synaptosomes of the mouse cerebral cortex at concentrations corresponding to those used clinically. The convulsive gas, DF-112, released glutamate more potently than the anesthetics. These data suggest the involvement of enhanced glutamate release in the mechanisms of excitatory effects of halogenated volatile anesthetics.
Hirose
T
T
Department of Anesthesiology, Kansai Medical University, Moriguchi.
Inoue
M
M
Uchida
M
M
Shingu
K
K
Inagaki
C
C
D018685:Anesthetics, Inhalation / Q000009:adverse effects*; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D017402:Chlorofluorocarbons / Q000009:adverse effects*; D004737:Enflurane / Q000009:adverse effects; D004987:Ethers / Q000009:adverse effects; D018698:Glutamic Acid / Q000378:metabolism* / Q000502:physiology; D006219:Halogens / Q000009:adverse effects*; D007530:Isoflurane / Q000009:adverse effects; D008297:Male; D008738:Methyl Ethers; D051379:Mice; D008815:Mice, Inbred Strains; D012640:Seizures / Q000139:chemically induced*; D000077149:Sevoflurane; D013035:Spasm / Q000139:chemically induced*; D013574:Synaptosomes / Q000378:metabolism
D018685:Anesthetics, Inhalation; D017402:Chlorofluorocarbons; D004987:Ethers; D006219:Halogens; D008738:Methyl Ethers; D000077149:Sevoflurane; D018698:Glutamic Acid; D004737:Enflurane; D007530:Isoflurane