Co-localization of beta-amyloid peptides, apolipoprotein E and glial markers in senile plaques in the prefrontal cortex of old rhesus monkeys.
Based on the homology of human and monkey amyloid precursor proteins and the derived beta-amyloid peptides (A beta) the investigation of brains from old monkeys might be useful for the understanding of beta-amyloidosis in the aetiology of Alzheimer's disease. In the present study, the prefrontal cortex, which is known to be highly susceptible to the deposition of A beta, was screened for the occurrence of senile plaques in perfused tissue of aged rhesus monkeys (Macaca mulatta). A beta deposits were immunocytochemically detected in five of six macaques aged about 28 years. Differently N-terminal truncated A beta species in the senile plaques were simultaneously detected by a carbocyanine double fluorescence method applying the bright red fluorescent Cy3 and the novel green fluorescent Cy2. In a few cases, immunoreactivity for the shortened fragment containing the amino acids 17-42 (A beta(17-42); p3 fragment with a molecular weight of 3 kDa) was demonstrated in deposits apparently devoid of A beta(8-17). Senile plaques were further characterized by carbocyanine double labelling of A beta and astrocytes, microglia and apolipoprotein E.
Härtig
W
W
Paul Flechsig Institute for Brain Research, University of Leipzig, Germany.
Brückner
G
G
Schmidt
C
C
Brauer
K
K
Bodewitz
G
G
Turner
J D
JD
Bigl
V
V
D000375:Aging / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D015415:Biomarkers; D002232:Carbocyanines; D004396:Coloring Agents; D008253:Macaca mulatta; D008297:Male; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism* / Q000473:pathology; D014018:Tissue Distribution
D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D015415:Biomarkers; D002232:Carbocyanines; D004396:Coloring Agents; D010446:Peptide Fragments