title: The ribosome at higher resolution--the donut takes shape.
abstract: Major new results in the 3D cryoimaging of ribosomes have advanced our understanding of ribosomal structure and function. For the first time, 3D difference maps have been used to image tRNA molecules in situ. With this new technology, the stage is set for detailed ligand-binding experiments that explore the binding states of elongation factors and tRNA, and that pinpoint locations of proteins and RNA on the surface of the ribosome.
authors.affiliation: Wadsworth Center, New York State Department of Health, State University of New York at Albany, PO Box 509, Empire State Plaza, Albany, NY 12201-0509, USA. joachim@wadsworth.org
authors.forename: J
authors.initials: J
authors.lastname: Frank
mesh_terms: D004926:Escherichia coli; D008854:Microscopy, Electron; D008958:Models, Molecular; D012335:RNA, Ribosomal / Q000032:analysis; D012269:Ribosomal Proteins / Q000032:analysis; D012270:Ribosomes / Q000648:ultrastructure*; D013499:Surface Properties
chemical_list: D012335:RNA, Ribosomal; D012269:Ribosomal Proteins