[Research of type 1, 2 and 3 hepatitis C virus specific antibodies in relation to the viral genotype in chronically infected patients].
The determination of the viral genotype in chronically HCV infected patients is used as an epidemiologic tool, a predictive marker of the evolution of the disease and especially of the response to the interferon alfa therapy. Its determination needs an amplification of the selected genomic region and remains expensive. Thus an indirect determination of the genotype has been proposed using the characterization of type-specific antibodies. In 101 chronically HCV infected patients, a serologic method specific for HCV type 1, 2 and 3 has been evaluated in relation to the genotyping one. The test was interpretable in 64 sera and in agreement with the genotyping method in 92% of the samples. The reactivity of the test was lower in hemodialysis and IVDUs patients (p < 0.02). The mixed results obtained by the serologic typing method were not in agreement with the results of the genotyping coinfections. We could not differentiate the possible past infections from the cross-reactivities of the test. The serotyping methods was simple and rapid, allowing generally a deduction of the viral genotype. It could be an attractive approach if the reactivity was improved and the subtyping was possible.
Castelain
S
S
Laboratoire de Virologie, Centre Hospitalo-Universitaire d'Amiens, Amiens, France.
Duverlie
G
G
Khorsi
H
H
Zawadzki
P
P
Capron
D
D
Capron
J P
JP
Sueur
J M
JM
Orfila
J
J
Eb
F
F
D000328:Adult; D002908:Chronic Disease; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000276:immunology; D006526:Hepatitis C / Q000235:genetics / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012703:Serotyping
D018937:Hepatitis C Antibodies
Recherche des anticorps spécifiques des types 1, 2 et 3 du virus de l'hépatite C en relation avec le génotype viral chez des patients chroniquement infectés.