title: Integrated approaches to the design of media and feeding strategies for fed-batch cultures of animal cells.
abstract: Animal cell culture has become an important approach for the production of biologically functional proteins for human therapy. The quantity and quality of protein production are influenced by the culture environment, which is subject to change over the course of cell cultivation. Therefore, it is vital to design an optimal culture environment and control it within an optimal region to maximize the productivity. This requires that the factors affecting the culture environment (nutrient concentrations, by-product accumulation, pH and osmolality) and cell growth be integrated into the design of culture media for fed-batch animal cell cultures.
authors.affiliation: Merck Research Laboratory Co., Inc., West Point, PA 19486, USA.
authors.forename: L
authors.initials: L
authors.lastname: Xie
authors.forename: D I
authors.initials: DI
authors.lastname: Wang
mesh_terms: D000818:Animals; D001709:Biotechnology; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins