A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.
The 569,750 base pair sequence corresponding to the 28.0-40.1 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. This region includes the replication terminus region and contained at least 549 potential open reading frames. Among them, 160 (29%) were previously reported, 174 (32%) were homologous to other known genes, 102 (18%) were identical or similar to hypothetical genes registered in databases, and the remaining 113 (21%) did not show a significant similarity to any other gene. Of interest was the finding of a large number of genes and gene clusters in and near the replication termination region which had been thought to be genetically silent. Those included a cluster of genes for fatty acid beta-oxidation, the third copy of the pot (spermidine/putrescine transport system) gene cluster, the second dpp (dipeptide transport system) operon, the second dsm (anaerobic dimethyl sulfoxide reductase) operon, a cluster of fim (fimbrial) genes and a DNA helicase-like gene with a high molecular weight. In addition, we found the dnaC- and dnaT-like genes in the cryptic prophage, Rac, and a number of genes originated probably from plasmids.
Aiba
H
H
Department of Molecular Biology, School of Science, Nagoya University, Japan.
Baba
T
T
Hayashi
K
K
Inada
T
T
Isono
K
K
Itoh
T
T
Kasai
H
H
Kashimoto
K
K
Kimura
S
S
Kitakawa
M
M
Kitagawa
M
M
Makino
K
K
Miki
T
T
Mizobuchi
K
K
Mori
H
H
Mori
T
T
Motomura
K
K
Nakade
S
S
Nakamura
Y
Y
Nashimoto
H
H
Nishio
Y
Y
Oshima
T
T
Saito
N
N
Sampei
G
G
Horiuchi
T
T
D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005810:Multigene Family; D016366:Open Reading Frames; D009876:Operon; D010957:Plasmids / Q000235:genetics; D012093:Replicon; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D017906:Siphoviridae / Q000235:genetics