Retinol, ascorbic acid and alpha-tocopherol prevent DNA adduct formation in mice treated with the mycotoxins ochratoxin A and zearalenone.
Ochratoxin A (OTA), and zearalenone (ZEN), two mycotoxins, have been implicated in numerous mycotoxicoses in farm animals and are genotoxic. Several adducts were detected in mouse and rat kidney after a single administration of OTA and in mice organs after zearalenone treatment which induces hepatocellular adenomas. The effects of some vitamins such as retinol (A), ascorbic acid (C) and alpha-tocopherol (E), which are known to act as superoxide anion scavengers, were tested on OTA genotoxicity. Pretreatment of mice by vitamin E decreased DNA adducts by 80% in kidney. Vitamin A decreased DNA adduct levels by 70% and Vitamin C by 90% in kidney. In the same way, pretreatment of female mice with alpha-tocopherol before administration of zearalenone inhibited significantly DNA adduct formation in liver and in kidney. The total DNA adduct level after E treatment was decreased by 45% and 58% in liver and kidney respectively.
Grosse
Y
Y
ENSAT, Laboratoire de Toxicologie et Sécurité Alimentaire, Toulouse, France.
Chekir-Ghedira
L
L
Huc
A
A
Obrecht-Pflumio
S
S
Dirheimer
G
G
Bacha
H
H
Pfohl-Leszkowicz
A
A
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D018736:DNA Adducts / Q000187:drug effects*; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000494:pharmacology; D009793:Ochratoxins / Q000494:pharmacology*; D014801:Vitamin A / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*; D015025:Zearalenone / Q000494:pharmacology*
D018736:DNA Adducts; D009183:Mycotoxins; D009793:Ochratoxins; D014801:Vitamin A; D014810:Vitamin E; C025589:ochratoxin A; D015025:Zearalenone; D001205:Ascorbic Acid