Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia.
Results from Southern hybridization and PCR amplification experiments using a randomly synthesized reverse transcription-PCR product showed that peripheral blood leukocytes from horses showing no clinical signs of disease expressed a putative latency-associated transcript antisense to and overlapping the 3' end of the equid herpesvirus 1 (EHV-1) immediate-early gene (gene 64). A PCR product derived from this transcript has > or =96% identity with the published EHV-1 sequence. In situ hybridization studies of equine bronchial lymph nodes corroborated these findings and are consistent with reactivation data (D. A. Smith, A. Hamblin, and N. Edington, unpublished data), indicating that EHV-1 latency is established predominantly in CD5+/CD8+ leukocytes.
Chesters
P M
PM
Department of Pathology and Infectious Diseases, The Royal Veterinary College, London, United Kingdom.
Allsop
R
R
Purewal
A
A
Edington
N
N
D000818:Animals; D015139:Blotting, Southern; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000502:physiology; D006736:Horses; D017403:In Situ Hybridization; D007962:Leukocytes / Q000821:virology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012668:Trigeminal Ganglion / Q000821:virology*; D017735:Virus Latency
D012333:RNA, Messenger