title: Effect of snuff on cytokeratin expression in oral vestibular sulcus epithelium.
abstract: Differences in the expression of cytokeratins (CK) in specimens obtained from snuff-affected oral epithelium of the maxillary vestibular sulcus and clinically normal sulcular epithelium were studied by indirect immunofluorescence staining with a panel of monoclonal antibodies (MAbs). CK 14, a marker of stratified squamous epithelium was not seen expressed in 3/11 of the snuff user's specimens. Terminal differentiation markers, typical of cornified epithelia (CK 1, 9, 10 and 11), were detected suprabasally in the snuff user's keratosis but not in the normal control epithelium. The use of snuff seemed to change the CK staining pattern of the mucosa so that it resembles more that of a cornified type of epithelium. Simple epithelial-type CK were included in the study in order to establish the CK profile of the snuff-induced keratosis, for comparison with normal and dysplastic lesions. MAb to CK 7 and 19 showed reactivity in the basal cells and suprabasally whereas the monospecific MAb anti-CK 7 showed suprabasal staining both in the control and affected epithelia. By using MAbs, we found no immunoreactivity against CK 18 either in normal or affected epithelia, whereas we found suprabasal reaction (5/11) against CK 8 in the snuff user's epithelia. The two MAbs demonstrating the expression of CK 19, normally confined to the basal cells of the stratified squamous epithelium, showed variable patterns of expression both in basal cells and suprabasally in the snuff lesions. The results show that use of oral snuff causes some alterations in the CK expression pattern of the affected epithelium. Whether the alterations are indicative of a premalignant change is, however, uncertain. The results encourage further studies on the subject.
authors.affiliation: Institute of Biomedicine, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Luomanen
authors.forename: O
authors.initials: O
authors.lastname: Tiitta
authors.forename: K
authors.initials: K
authors.lastname: Heikinheimo
authors.forename: I
authors.initials: I
authors.lastname: Heinaro
authors.forename: R P
authors.initials: RP
authors.lastname: Happonen
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D016650:Fluorescein-5-isothiocyanate; D019085:Fluorescent Antibody Technique, Direct; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation; D006801:Humans; D006965:Hyperplasia; D007633:Keratins / Q000032:analysis / Q000235:genetics*; D007972:Leukoplakia, Oral / Q000378:metabolism / Q000473:pathology; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism* / Q000473:pathology; D010947:Plants, Toxic; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D014030:Tobacco, Smokeless
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D005456:Fluorescent Dyes; D007633:Keratins; D016650:Fluorescein-5-isothiocyanate