The origin of transfer (oriT) of the enterococcal, pheromone-responding, cytolysin plasmid pAD1 is located within the repA determinant.
The highly conjugative pAD1 plasmid encodes a mating response to the peptide sex pheromone cAD1 secreted by recipient strains of Enterococcus faecalis. The location of the origin of transfer (oriT) was determined through the ability to mobilize the Escherichia coli-E. faecalis shuttle plasmid pAM401 carrying specific segments of pAD1. The oriT sequence was found to be completely within the repA determinant known to be required for plasmid maintenance.
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School of Dentistry, The University of Michigan, Ann Arbor, Michigan 48109, USA.
Clewell
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DB
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D004265:DNA Helicases; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D018741:Replication Origin; D012724:Sex Attractants / Q000235:genetics; D015534:Trans-Activators
D001426:Bacterial Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D009842:Oligopeptides; D011506:Proteins; D012724:Sex Attractants; D015534:Trans-Activators; C065937:aggregation substance, Enterococcus faecalis; C045264:replication initiator protein; C043691:cAD1 bacterial sex hormone; D004265:DNA Helicases