title: Cardiopulmonary bypass and circulatory arrest increase endothelin-1 production and receptor expression in the lung.
abstract: BACKGROUND
  Endothelin-1 has been shown to be a mediator of pulmonary hypertension after cardiopulmonary bypass and deep hypothermic circulatory arrest. It is not known whether the mechanism is increased production of endothelin-1 or alterations in expression of endothelin-1 receptors in the lung. This study was designed to test the hypothesis that circulatory arrest increases endothelin-1 mRNA levels and endothelin-1 receptor expression in the lung.
  
  
  RESULTS
  Twenty-four piglets (7 to 30 days old) were studied randomly either at baseline (controls, n = 12) or after cardiopulmonary bypass with 30 minutes of circulatory arrest (deep hypothermic circulatory arrest, n = 12). Lungs and pulmonary arteries were harvested immediately after hemodynamic data collection. Deep hypothermic circulatory arrest significantly increased pulmonary vascular resistance (p < 0.01). Deep hypothermic circulatory arrest also produced a significant increase in endothelin-1 mRNA levels in the pulmonary arteries (149 +/- 55 pg vs 547 +/- 111 pg, p = 0.007). There was no significant change in the pulmonary parenchymal endothelin-1 mRNA levels (4102 +/- 379 pg vs 4623 +/- 308 pg, p = 0.32). Ligand binding studies of the lung parenchyma revealed a single specific endothelin-1 binding site with an EC50 value (effective concentration causing 50% of the maximum response) of about 1 x 10(-8) mol/L, consistent with the endothelin B subtype. Deep hypothermic circulatory arrest resulted in a significant increase in the number of endothelin-1 receptors in the lung (109 +/- 6 fmol/mg total protein to 135 +/- 9 fmol/mg total protein, p = 0.02).
  
  
  CONCLUSIONS
  Deep hypothermic circulatory arrest increases production of endothelin-1 by the pulmonary vascular endothelium. Endothelin-1 production in the pulmonary parenchyma does not change. Expression of endothelin B receptors in the pulmonary parenchyma also increases after cardiopulmonary bypass with deep hypothermic circulatory arrest. This study supports the hypothesis that deep hypothermic circulatory arrest results in pulmonary vascular endothelial activation with increased endothelin-1 mRNA production.
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, N.C., USA.
authors.forename: P M
authors.initials: PM
authors.lastname: Kirshbom
authors.forename: S O
authors.initials: SO
authors.lastname: Page
authors.forename: M T
authors.initials: MT
authors.lastname: Jacobs
authors.forename: S S
authors.initials: SS
authors.lastname: Tsui
authors.forename: E
authors.initials: E
authors.lastname: Bello
authors.forename: R M
authors.initials: RM
authors.lastname: Ungerleider
authors.forename: D A
authors.initials: DA
authors.lastname: Schwinn
authors.forename: J W
authors.initials: JW
authors.lastname: Gaynor
mesh_terms: D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D019332:Endothelin-1 / Q000502:physiology*; D005786:Gene Expression Regulation; D006324:Heart Arrest, Induced / Q000009:adverse effects*; D006439:Hemodynamics; D006976:Hypertension, Pulmonary / Q000097:blood / Q000209:etiology*; D008168:Lung / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D017466:Receptors, Endothelin / Q000502:physiology*; D013552:Swine
chemical_list: D019332:Endothelin-1; D012333:RNA, Messenger; D017466:Receptors, Endothelin