Lactogenic hormones of the placenta and pituitary inhibit suckling-induced prolactin (PRL) release but not the ante-partum PRL surge.
Prolactin (PRL) and other lactogenic hormones feed back at the hypothalamus to inhibit PRL release. At midpregnancy, high circulating levels of placental lactogens (PL) terminate the mating-induced biphasic PRL surges in female rats. In the dark period preceding parturition, however, an ante-partum PRL surge occurs despite continuously high levels of PL. This study examined whether the lactogenic hormone negative feedback loop is altered during the ante-partum surge using two models: (i) pregnant rats given a hypothalamic implant of albumin, ovine PRL, or recombinant rat PL-I on Day 19 or 20 of pregnancy; and (ii) pregnant rats bearing a transplant of a rat choriocarcinoma cell line, Rcho-1 (PL-secreting), or HRP-1 (non-PL-secreting). Serial blood samples were taken via carotid cannula from all rats. Although lactogenic hormones placed in the hypothalamus reduced suckling-induced PRL release by 89%, hypothalamic implants of oPRL or recombinant rPL-I did not attenuate the ante-partum PRL surge. Rcho-transplanted rats also did not have a significantly reduced ante-partum PRL surge (peak PRL level, 131 ng/ml) compared with HRP-bearing rats (peak PRL level, 107 ng/ml). Northern blot analysis revealed that the Rcho-1 tumors expressed both PL-I and PL-II, while the HRP-1 tumors did not express either PL. The inability of the Rcho-1 transplants to inhibit the ante-partum PRL surge suggests that lactogenic hormone negative feedback is disrupted during the ante-partum period, possibly by the changing steroid profile associated with parturition.
Fliestra
R J
RJ
Department of Physiology, Kansas University Medical Center, Kansas City 66160-7401, USA.
Voogt
J L
JL
D000818:Animals; D000833:Animals, Suckling; D001835:Body Weight; D002822:Choriocarcinoma / Q000737:chemistry; D005246:Feedback; D005260:Female; D007031:Hypothalamus; D007774:Lactation / Q000502:physiology*; D009368:Neoplasm Transplantation; D010928:Placental Lactogen / Q000235:genetics / Q000494:pharmacology*; D011247:Pregnancy; D011388:Prolactin / Q000097:blood* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012756:Sheep; D014594:Uterine Neoplasms / Q000737:chemistry
D012333:RNA, Messenger; D012334:RNA, Neoplasm; C085699:placental lactogen I, rat; D011388:Prolactin; D010928:Placental Lactogen