The relevance of c-myc to the physiology of the human ovary.
Despite the potential importance of c-myc protein to cell proliferation and apoptosis, nothing is known about the contribution of this proto-oncogene to the development of the ovarian follicles in humans. Therefore the localization of c-myc in the human ovary was the main aim of this study. Localization of c-myc mRNA in 15 human ovaries was assessed by in situ hybridization. Atresia of the antral follicles was determined by in situ 3'-end labeling of fragmented DNA. Proto-oncogene c-myc was found in all the stages of follicular development except the primordial stage. The corpus luteum was also positive for c-myc mRNA. There was no positive staining for c-myc in either the corpus albicans or the postmenopausal ovary. These data indicate that the expression of c-myc may play a role in the molecular mechanisms of cell proliferation and apoptosis in the human ovary.
Putowski
L T
LT
2nd Department of Surgical Gynecology, University School of Medicine, Lublin, Poland.
Skrzypczak
M
M
Zielewicz
J
J
Kaminski
K
K
Jakowicki
J A
JA
D000328:Adult; D003338:Corpus Luteum / Q000737:chemistry; D053938:DNA Fragmentation; D005260:Female; D005496:Follicular Atresia; D006107:Granulosa Cells / Q000737:chemistry; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D006080:Ovarian Follicle / Q000737:chemistry; D010053:Ovary / Q000737:chemistry / Q000502:physiology*; D017698:Postmenopause; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D013799:Theca Cells / Q000737:chemistry
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger