Preparation of succinimidyl and pentafluorophenyl active esters of 5- and 6-carboxyfluorescein.
A mixture of 5- and 6-carboxyfluorescein was activated with 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride in the presence of either N-hydroxysuccinimide or pentafluorophenol to give the corresponding succinimidyl and pentafluorophenyl esters. The regioisomeric mixtures were separated to give the 5- and 6-succinimidyl and pentafluorophenyl active esters in > 98% purity.
Adamczyk
M
M
Diagnostic Division, Abbott Laboratories, Abbott Park, IL 60064-3500, USA. adamczykm@apmac.abbott.com
Fishpaugh
J R
JR
Heuser
K J
KJ
D004951:Esterification; D005452:Fluoresceins / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D005464:Fluorobenzenes; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010636:Phenols; D013388:Succinimides; D005466:Fluorocarbons
D005452:Fluoresceins; D005456:Fluorescent Dyes; D005464:Fluorobenzenes; D010636:Phenols; D013388:Succinimides; C024098:6-carboxyfluorescein; C085539:2,3,4,5,6-pentafluorophenol; D005466:Fluorocarbons; C001426:N-hydroxysuccinimide