Characterization of P0, a ribosomal phosphoprotein of Plasmodium falciparum. Antibody against amino-terminal domain inhibits parasite growth.
A cDNA expression clone of the human malarial parasite Plasmodium falciparum, lambdaPf4, which was reactive only to the immune sera and not to the patient sera, has recently been found to be the P. falciparum homologue of the P0 ribosomal phosphoprotein gene. A Northern analysis of the P0 gene revealed the presence of two transcripts, both present in all the different intraerythrocytic stages of the parasite life cycle. A 138-base pair amino-terminal domain of this gene was expressed as a fusion protein with glutathione S-transferase in Escherichia coli. Polyclonal antibodies raised against this domain immunoprecipitated the expected 38-kDa P0 protein from the 35S-labeled as well as 32P-labeled P. falciparum cultures. Monospecific human immune sera affinity-purified using the expression clone lambdaPf4 also immunoprecipitated the same size protein from [35S]methionine-labeled P. falciparum protein extract. Purified IgG from polyclonal antibodies raised against the amino-terminal domain of P0 protein completely inhibited the growth of P. falciparum in vitro. This inhibition appears to be mainly at the step of erythrocyte invasion by the parasites.
Goswami
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Molecular Biology Unit, Tata Institute of Fundamental Research, Homi Bhabha Road, Bombay, 400 005 India.
Singh
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Redkar
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Sharma
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D000818:Animals; D000913:Antibodies, Protozoan / Q000494:pharmacology*; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000469:parasitology; D004926:Escherichia coli; D019084:Fluorescent Antibody Technique, Indirect; D017125:Genes, Protozoan; D006801:Humans; D007700:Kinetics; D016778:Malaria, Falciparum / Q000469:parasitology; D010750:Phosphoproteins / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D010963:Plasmodium falciparum / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D015800:Protozoan Proteins / Q000502:physiology*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*
D000913:Antibodies, Protozoan; D010750:Phosphoproteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; C054675:ribosomal protein P0