A vector for expressing foreign genes in the brains and hearts of transgenic mice.
An expression plasmid (MoPrP.Xho), for use in transgenic mice, was developed from the promoter, 5' intronic, and 3' untranslated sequences of the murine prion protein gene. Analyses of mice harboring the MoPrP.Xho construct with cDNA genes encoding the amyloid precursor protein (APP) and human presenilin 1 demonstrated that this vector provides relatively high levels of transgene-encoded polypeptides in brains and hearts of transgenic mice. The MoPrP.Xho vector should be very useful in strategies designed to overexpress a variety of wild-type and disease related mutant transgenes in the heart and brain.
Borchelt
D R
DR
Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD 21205-2196, USA. drbor@welchlink.welch.jhu.edu
Davis
J
J
Fischer
M
M
Lee
M K
MK
Slunt
H H
HH
Ratovitsky
T
T
Regard
J
J
Copeland
N G
NG
Jenkins
N A
NA
Sisodia
S S
SS
Price
D L
DL
D016564:Amyloid beta-Protein Precursor / Q000032:analysis / Q000235:genetics; D000818:Animals; D001253:Astrocytes / Q000737:chemistry; D001923:Brain Chemistry; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic / Q000235:genetics*; D009206:Myocardium / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry; D009928:Organ Specificity; D053764:Presenilin-1; D011328:Prions / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
D016564:Amyloid beta-Protein Precursor; D008565:Membrane Proteins; C508853:PSEN1 protein, human; D053764:Presenilin-1; D011328:Prions; D012333:RNA, Messenger