title: Rapid and sensitive immunomagnetic separation-polymerase chain reaction method for the detection of Escherichia coli O157:H7 in raw milk and ice-cream.
abstract: Escherichia coli O157:H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157:H7/g food could be detected in < 10 h.
authors.affiliation: CIFAR, Antibody Engineering Laboratory, University of California, Davis 95616-8584, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Gooding
authors.forename: P V
authors.initials: PV
authors.lastname: Choudary
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D007054:Ice Cream / Q000382:microbiology*; D018189:Immunomagnetic Separation; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D022622:Shiga Toxin 1
chemical_list: D001427:Bacterial Toxins; D022622:Shiga Toxin 1