The organization of the human immunoglobulin lambda gene locus.
To elucidate the complex structure of the human immunoglobulin lambda gene locus, a 1020-kb contig was constructed using 184 cosmid clones and one bacterial artificial chromosome (BAC) clone. A high-resolution physical map of this contig revealed that the entire lambda gene locus is 911 kb in length. It contains seven constant region (C lambda) gene segments and 69 unique EcoRI-HindIII segments that hybridize to variable region gene (V lambda) probes. The VpreB gene, BCRL4, and gamma-glutamyl transpeptidase gene (GGT)-like sequences are also located within the lambda gene locus. Hybridization analysis suggested that the lambda gene locus has undergone extensive amplification events in evolution.
Kawasaki
K
K
Department of Molecular Biology, Keio University, School of Medicine, Tokyo, Japan.
Minoshima
S
S
Schooler
K
K
Kudoh
J
J
Asakawa
S
S
de Jong
P J
PJ
Shimizu
N
N
D001483:Base Sequence; D002874:Chromosome Mapping; D016386:Chromosome Walking; D018244:Chromosomes, Artificial, Yeast; D002892:Chromosomes, Human, Pair 22; D003360:Cosmids; D017931:DNA Primers; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D008969:Molecular Sequence Data
D017931:DNA Primers; D007146:Immunoglobulin lambda-Chains