Regulation by oestrogens of tachykinin NK3 receptor expression in the rat uterus.
The expression of the tachykinin NK3 receptor and its regulation by ovarian steroids were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) in uteri from ovariectomized rats. A single transcript corresponding to the 325-bp product expected for the tachykinin NK3 receptor was detected in uteri from olive oil-treated (control) ovariectomized rats. The level of tachykinin NK3 receptor mRNA in progesterone-treated animals was similar to that observed in uteri from control ones. Tachykinin NK3 receptor mRNA levels were significantly smaller in uteri from oestrogen-treated ovariectomized rats, with approximately a 32-fold decrease. These findings suggest that oestrogen, but not progesterone, regulates the expression of tachykinin NK3 receptors in the rat uterus.
Pinto
F M
FM
Instituto de Investigaciones Científicas Isla de La Cartuja, Sevilla, Spain.
Magraner
J
J
Ausina
P
P
Anselmi
E
E
Martín
J D
JD
Candenas
M L
ML
D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D010052:Ovariectomy; D016133:Polymerase Chain Reaction; D011374:Progesterone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018042:Receptors, Neurokinin-3 / Q000187:drug effects* / Q000235:genetics; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D018076:DNA, Complementary; D004967:Estrogens; D012333:RNA, Messenger; D018042:Receptors, Neurokinin-3; D011374:Progesterone