In vitro platelet function in controlled ovarian hyperstimulation cycles.
OBJECTIVE
To determine the effects of elevated endogenous E2 levels on in vitro platelet function in patients undergoing controlled ovarian hyperstimulation (COH).


METHODS
Women with normal ovulatory cycles and patients undergoing COH on cycle day 3 and near ovulation (preovulatory follicles were at least 16 mm in diameter) were studied. Serum E2, Thrombostat 4000, (V. d. Goltz, Seeon, Germany), von Willebrand factor antigen (vWF-Ag), and platelet aggregation and adenosine triphosphate (ATP) release to adenosine diphosphate (ADP), collagen (COL), and arachidonic acid (AA) were measured.


METHODS
University-based outpatient infertility clinic.


METHODS
Twenty-two consenting infertile women undergoing COH cycles and 14 women with documented ovulatory cycles.


METHODS
Whole blood platelet aggregation with ADP, COL, AA, and Thrombostat 4000.


RESULTS
Estradiol levels rose significantly at peak times (P = 0.011). No changes were noted in in vitro platelet function measured by the Thrombostat 4000 and by whole blood platelet aggregation with ADP and AA and in ATP release with ADP, COL, or AA. Aggregation with collagen was increased because of likely elevations in vWF-Ag levels.


CONCLUSIONS
No significant changes in in vitro platelet function were noted in 19 women undergoing COH with E2 levels two to three times that observed in oral contraceptive or hormone replacement therapy users, suggesting no increased risk for arterial thromboembolism.
Richard-Davis
G
G
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan, USA.
Montgomery-Rice
V
V
Mammen
E F
EF
Alshameeri
R S
RS
Morgan
D
D
Moghissi
K S
KS
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D016718:Arachidonic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D003094:Collagen / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D010062:Ovulation Induction; D010974:Platelet Aggregation / Q000187:drug effects; D014841:von Willebrand Factor / Q000378:metabolism
D014841:von Willebrand Factor; D016718:Arachidonic Acid; D004958:Estradiol; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003094:Collagen