title: Pollination-enhanced expression of a receptor-like protein kinase related gene in tobacco styles.
abstract: We have isolated a putative serine/threonine receptor kinase gene with an expression pattern indicating that it may play a role in the stylar response to pollination. Differential display PCR was used to select tobacco mRNAs with increased accumulation following pollination. NTS16, a cDNA identified by this method, is homologous to a ca. 2.4 kb mRNA primarily expressed in pistil tissues. Levels of this mRNA increase during floral development and are further increased by pollination reaching maximal accumulation 12-18 hours after pollination and then declining. mRNA levels can also be increased by the application of ethylene to unpollinated flowers. A polypeptide encoded by the NTS 16 open reading frame has sequence similarity to the catalytic domain of several receptor protein kinases from plants including the S-receptor kinases implicated in the rejection of self-pollen in Brassica species and the Pto gene product of tomato which confers resistance to a bacterial pathogen.
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
authors.forename: H Y
authors.initials: HY
authors.lastname: Li
authors.forename: J E
authors.initials: JE
authors.lastname: Gray
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D010947:Plants, Toxic; D011058:Pollen / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000302:isolation & purification; D018749:RNA, Plant / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D012098:Reproduction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development
chemical_list: D018076:DNA, Complementary; D005030:Ethylenes; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011956:Receptors, Cell Surface; C036216:ethylene; D017346:Protein Serine-Threonine Kinases