title: An association between HIV-1 subtypes and mode of transmission in Cape Town, South Africa.
abstract: OBJECTIVE
  To determine HIV-1 env and gag subtypes in male homosexual and heterosexual populations in Cape Town, South Africa.
  
  
  METHODS
  DNA was isolated from blood originating from 61 patients attending local clinics. Samples were divided according to presumed mode of transmission: male homosexual (n = 26), heterosexual/vertical (n = 32), blood transfusion (n = 1) and unknown (n = 2).
  
  
  METHODS
  Proviral HIV-1 DNA was subtyped by heteroduplex mobility assay (HMA) based on the 799 base-pair V3-V5 region of the env gene (n = 47) or by sequence analysis of the p17 region of the gag gene (n = 33), or both. For HMA, reference plasmids were constructed containing the V1-V5 env region sequences (1.2-kb) representative of local subtypes. Subtype designation of reference subtypes was confirmed by sequence analysis of the V3-loop region.
  
  
  RESULTS
  Analysis of the partial gag sequences and HMA of the V3-V5 env region identified three subtypes: B, C and D. A fourth env subtype, subtype E, was also identified by HMA. Subtypes were found to segregate according to mode of transmission, with subtype B viruses found in 96% (25 out of 26) of the male homosexual group and subtype C viruses found in 81% (26 out of 32) of the heterosexual/vertical transmission group. Subtype B viruses were also found in four heterosexual patients, one patient infected by blood transfusion and in two patients with unknown mode of transmission. Subtype D viruses were found in one male homosexual patient and one heterosexual patient. A subtype E virus was identified in a heterosexual patient. No discrepancy was found in subtype designation in samples analysed in both between the gag and env regions (n = 19).
  
  
  CONCLUSIONS
  Subtype B viruses were associated with male homosexual transmission and subtype C viruses with heterosexual transmission, suggesting two independent epidemics. This data may have implications in the selection of appropriate vaccines for different risk groups in the country.
authors.affiliation: Department of Medical Microbiology, University of Cape Town, Observatory, South Africa.
authors.forename: J
authors.initials: J
authors.lastname: van Harmelen
authors.forename: R
authors.initials: R
authors.lastname: Wood
authors.forename: M
authors.initials: M
authors.lastname: Lambrick
authors.forename: E P
authors.initials: EP
authors.lastname: Rybicki
authors.forename: A L
authors.initials: AL
authors.lastname: Williamson
authors.forename: C
authors.initials: C
authors.lastname: Williamson
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005260:Female; D015752:Genes, env / Q000235:genetics; D015750:Genes, gag / Q000235:genetics; D015658:HIV Infections / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D018451:Homosexuality, Male; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009692:Nucleic Acid Heteroduplexes; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013019:South Africa
chemical_list: D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes