title: Philadelphia chromosome in chronic myelogenous leukemia: confirmation of cytogenetic diagnosis in Ph positive and negative cases by fluorescence in situ hybridization.
abstract: In this study, patients with Philadelphia (Ph) negative and positive chronic myelogenous leukemia (CML) were analyzed by unicolor- and dual color- (DC) fluorescence in situ hybridization (FISH) using abl and bcr cosmid probes. Unicolor- and DC-FISH analysis revealed a BCR-ABL fusion in a Ph-negative patient. DC-FISH to interphase nuclei revealed the BCR-ABL fusion, even though no metaphases were available for metaphase-FISH and conventional cytogenetic analysis. In the remaining cases, the findings of FISH were in agreement with the cytogenetic results.
authors.affiliation: Department of Medical Genetics, University of Selçuk, Konya, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Acar
authors.forename: J
authors.initials: J
authors.lastname: Stewart
authors.forename: M J
authors.initials: MJ
authors.lastname: Connor
mesh_terms: D004273:DNA, Neoplasm / Q000235:genetics; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D016313:Genes, abl; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D004273:DNA, Neoplasm; D016044:Fusion Proteins, bcr-abl