Terminal oxidases of Azoarcus sp. BH72, a strictly respiratory diazotroph.
The nitrogen-fixing, grass-associated bacterium Azoarcus sp. BH72 was characterized with respect to its terminal oxidases. Inhibitory respiratory analysis revealed the presence of at least one cytochrome c oxidase and one quinol oxidase. The cytochrome c oxidase was preferably used by the cells under aerobic, whereas the quinol oxidase seemed to be dominant under microaerobic, nitrogen-fixing conditions. Differential spectroscopy and heme analysis of the membrane preparations indicated that the cytochrome c oxidase is probably of the cb type.
Reinhold-Hurek
B
B
Max-Planck-Institut fur terrestrische Mikrobiologie, Arbeitsgruppe Symbioseforschung, Marburg, Germany. reinhold@mailer.uni-marburg.de
Zhulin
I B
IB
D002851:Chromatography, High Pressure Liquid; D014450:Electron Transport Complex III / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000201:enzymology* / Q000254:growth & development; D006418:Heme / Q000302:isolation & purification; D009586:Nitrogen Fixation; D010088:Oxidoreductases / Q000378:metabolism*; D010101:Oxygen Consumption; D006109:Poaceae / Q000382:microbiology; D013053:Spectrophotometry
D006418:Heme; D010088:Oxidoreductases; C020094:duroquinol oxidase; D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III