Binucleation and polyploidization patterns in developmental and regenerative rat liver growth.
The hepatocellular binucleation rate, measured as the percentage of binuclear cells amongst newly formed bromodeoxyuridine-labelled and immunostained collagenase-isolated rat hepatocytes, decreased from 12% to 4% between days 30 and 40 after birth, rose to 20% between days 50 and 60, and then declined again to the adult rate of about 10% at day 80. During regenerative growth following a two-thirds partial hepatectomy, the rate of binucleation declined to about 3%, causing the fraction of binuclear cells to fall from 27% (before hepactectomy) to 5% (at 45 h after hepactectomy) as pre-existing binuclear cells replicated and formed mononuclear daughter cells. Essentially all (97%) hepatocytes replicated at least once, starting their DNA synthesis at around 13 h and reaching a peak at 30 h, irrespective of ploidy and nuclearity. At later time points, the diploid hepatocytes had a higher labelling index than the polyploid cells, suggesting a greater tendency to go through several cell cycles.
Gerlyng
P
P
Department of Tissue Culture, Institute for Cancer Research the Norwegian Radium Hospital, Oslo.
Abyholm
A
A
Grotmol
T
T
Erikstein
B
B
Huitfeldt
H S
HS
Stokke
T
T
Seglen
P O
PO
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure*; D002469:Cell Separation; D008099:Liver / Q000166:cytology*; D008115:Liver Regeneration; D008297:Male; D011003:Ploidies; D051381:Rats; D011921:Rats, Inbred WKY
D001973:Bromodeoxyuridine