Macrophage surface glycoproteins binding to galectin-3 (Mac-2-antigen).
Galectin-3 (formerly called Mac-2 antigen) is a approximately 30 kDa carbohydrate-binding protein expressed on the surface of inflammatory macrophages and several macrophage cell lines. We have purified from lysates of the murine macrophage cell line WEHI-3 glycoproteins that bind to a galectin-3 affinity column. Several of these receptors are labelled after biotinylation of intact cells showing their location at the cell surface. N-terminal aminoacid sequencing of intact galectin-3-binding glycoproteins isolated from preparative SDS-gels or of chemically derived fragments showed several homologies with known proteins and identification was confirmed by immunoprecipitation with specific antibodies. The glycoproteins were shown to be: the alpha-subunit(CD11b) of the CD11b/CD18 integrin(Mac-1 antigen); the lysosomal membrane glycoproteins LAMPs 1 and 2 which are known in part to be expressed at cell surfaces; the Mac-3 antigen, a mouse macrophage differentiation antigen defined by the M3/84 monoclonal antibody and related immunochemically to LAMP-2; the heavy chain of CD98, a 125 kDa heterodimeric glycoprotein identified by the 4F2/RL388 monoclonal antibodies respectively on human and mouse monocytes/macrophages and on activated T cells. Further studies showed that CD11b/CD18, CD98 and Mac-3 are major surface receptors for galectin-3 on murine peritoneal macrophages elicited by thioglycollate.
Dong
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National Institute for Medical Research, Mill Hill, London, UK.
Hughes
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RC
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002846:Chromatography, Affinity; D005260:Female; D027261:Fusion Regulatory Protein-1; D037502:Galectin 3; D051907:Lysosomal Membrane Proteins; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008264:Macrophages / Q000737:chemistry* / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D051381:Rats; D017421:Sequence Analysis
D015703:Antigens, CD; D000943:Antigens, Differentiation; D002352:Carrier Proteins; D027261:Fusion Regulatory Protein-1; D037502:Galectin 3; D051907:Lysosomal Membrane Proteins; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; C036763:monocyte-macrophage differentiation antigen