title: Endothelium on the flow surface of human aortic Dacron vascular grafts.
abstract: OBJECTIVE
  We have previously observed endothelium on two human vascular prostheses explanted under optimal conditions for flow surface preservation. In this study we sought to further verify the hypothesis that endothelialization can occur on clinical grafts and that it can be detected in specimens that have been promptly removed and properly preserved in a timely manner.
  
  
  METHODS
  We studied 29 aortic grafts. Of these, 11 Dacron bypass grafts were in a condition suitable for analysis with light microscopy and immunocytochemistry staining, and scanning and transmission electron microscopy.
  
  
  RESULTS
  Three grafts had endothelium beyond the pannus, identified by factor VIII/ vWF, Ulex europaeus agglutinin, and collagen IV positivity. Specimen A, a knitted 6-year implant, was preserved by embalming 3 hours after the patient's death and had a firmly attached outer capsule with fibroblasts, collagen, giant cells, and microvessels in the interstices. Specimen B, a woven 18-year implant, was retrieved at reoperation and immediately fixed in 10% formalin; it had no outer capsule and no tissue ingrowth. Specimen C, a woven 7-year implant, was removed and fixed 5 hours after the patient's death; it had a firmly attached outer capsule but no tissue ingrowth beyond the outer portion of the wall.
  
  
  CONCLUSIONS
  The rapidity with which the specimens were fixed probably enabled identification of endothelium. These findings suggest that endothelialization of synthetic arterial grafts may occur more frequently in human beings than previously recognized.
authors.affiliation: Hope Heart Institute, Seattle, WA 98122, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.forename: M H
authors.initials: MH
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Onuki
authors.forename: R
authors.initials: R
authors.lastname: Ghali
authors.forename: G C
authors.initials: GC
authors.lastname: Hunter
authors.forename: K H
authors.initials: KH
authors.lastname: Johansen
authors.forename: L R
authors.initials: LR
authors.lastname: Sauvage
mesh_terms: D000368:Aged; D001011:Aorta / Q000601:surgery*; D017544:Aortic Aneurysm, Abdominal / Q000601:surgery; D001807:Blood Vessel Prosthesis; D003094:Collagen / Q000032:analysis; D004396:Coloring Agents; D003238:Connective Tissue / Q000033:anatomy & histology; D004730:Endothelium, Vascular / Q000033:anatomy & histology* / Q000648:ultrastructure; D005169:Factor VIII / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology; D015726:Giant Cells / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000032:analysis; D008297:Male; D008833:Microcirculation; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D037121:Plant Lectins; D011093:Polyethylene Terephthalates; D012039:Regional Blood Flow; D013048:Specimen Handling; D013499:Surface Properties; D013997:Time Factors; D014021:Tissue Preservation; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: D004396:Coloring Agents; D037102:Lectins; D037121:Plant Lectins; D011093:Polyethylene Terephthalates; C036850:Ulex europaeus lectins; D014841:von Willebrand Factor; D005169:Factor VIII; D003094:Collagen