Preparative electrophoresis in a concentrated polymer solution: automated procedure for microsome isolation.
A recently introduced automated gel electrophoresis apparatus with an intermittent scanning of the migration path, the HPGE 1000 apparatus (LabIntelligence, Belmont, CA) has a unique preparative feature: A recovery cup can be automatically positioned on the band of interest, which is then electroeluted with fluorescence monitoring of recovery. Electroelution time is extended stepwise until quantitative recovery is attained. This preparative procedure has been extended from electrophoresis in gels to that of subcellularsized particles in concentrated polymer solutions Chang et al., Electrophoresis 17, 776-780, 1996). However, that application required manual rather than computer-directed positioning of the recovery chamber over the band. The present report details a modification of instrumentation and procedure by which automated operation was restored. The modified procedure extends the benefits of automation and known quantitative recovery to isolation of particles too large to enter into gels.
Chang
H T
HT
Department of Chemistry, National Taiwan University, Taipei, Republic of China.
Chen
N
N
Sullivan
J V
JV
Chrambach
A
A
D000818:Animals; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D003201:Computers; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D008862:Microsomes, Liver / Q000648:ultrastructure*; D011205:Povidone; D051381:Rats; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
D012996:Solutions; D011205:Povidone