title: Effects of coffee cherry on the activation of splenic lymphocytes in mice.
abstract: We previously observed that the ingestion by mice of a hot water extract (CC) and the methanol-extracted and water-soluble fraction (CC-W) of coffee cherry, the residue remaining after the removal of coffee beans from the fruit, enhanced the differentiation of thymocytes and the activation of peripheral T-lymphocytes; and the anti-mammary tumour effects of coffee cherry extract was considered to be associated with this immunomodulation. To study further these effects, mitogen response and some immune parameters were examined in a high mammary tumour strain of SHN mice. While the T-lymphocyte response to concanavalin A was not significantly changed by either CC or CC-W, the lipopolysaccharide response was significantly enhanced by both treatments. The proportion of CD45R/B220+ (B) cells in the splenic lymphocytes was significantly increased by CC, and the percentage of activated (CD25+) cells in B-lymphocytes was increased by CC and CC-W. These data indicate that coffee cherry extract can enhance B-lymphocyte response, and suggest that this immunopotentiation contributes to the antitumourigenic role of coffee cherry.
authors.affiliation: Experimental Animal Research Laboratory, Meiji University, Kawasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Yasuda
authors.forename: K
authors.initials: K
authors.lastname: Iijima
authors.forename: K
authors.initials: K
authors.lastname: Toriizuka
authors.forename: J C
authors.initials: JC
authors.lastname: Cyong
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D003069:Coffee; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D003069:Coffee; D010936:Plant Extracts