Expression patterns of vascular-specific promoters RolC and Sh in transgenic potatoes and their use in engineering PLRV-resistant plants.
The expression patterns of GUS fusion constructs driven by the Agrobacterium rhizogenes RolC and the maize Sh (Shrunken; sucrose synthase-1) promoters were examined in transgenic potatoes (cv. Atlantic). RolC drove high-level gene expression in phloem tissue, bundle sheath cells and vascular parenchyma, but not in xylem or non-vascular tissues. Sh expression was exclusively confined to phloem tissue. Potato leafroll luteovirus (PLRV) replicates only in phloem tissues, and we show that when RolC is used to drive expression of the PLRV coat protein gene, virus-resistant lines can be obtained. In contrast, no significant resistance was observed when the Sh promoter was used.
Graham
M W
MW
CSIRO Division of Plant Industry, Canberra, Australia.
Craig
S
S
Waterhouse
P M
PM
D000818:Animals; D001042:Aphids / Q000821:virology; D001426:Bacterial Proteins / Q000235:genetics; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005964:Glucosyltransferases / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics; D017793:Luteovirus / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011198:Solanum tuberosum / Q000821:virology*; D014018:Tissue Distribution; D001617:beta-Glucosidase
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D005964:Glucosyltransferases; C022699:sucrose synthase; C071257:cytokinin-beta-glucosidase; D001617:beta-Glucosidase