title: Glutamyl endopeptidase of Bacillus intermedius, strain 3-19.
abstract: A homogeneous glutamyl endopeptidase splitting peptide bonds of glutamic, rarely of aspartic acid residues in peptides and proteins, was isolated from Bacillus intermedius 3-19 culture filtrate using chromatography on CM cellulose and Mono S. The enzyme molecular mass is equal to 29 kDa, pI 8.4. The protease is inhibited by diisopropylfluorophosphate. The enzyme, like other glutamyl endopeptidases, reveals two pH optima at pH 7.5 and 9.0 for casein and one at pH 8.0 for Z-Glu-pNA hydrolysis. A 6 mM K(m) is found for hydrolysis of the latter substrate. The enzyme activity optimum lies at 55 degrees C, and it is stable at pH 6.5-11.0. Its N-terminal sequence shows 56% coinciding residues when compared with that of Bacillus licheniformis glutamyl endopeptidase.
authors.affiliation: Department of Biology, Kazan State University, Russia. root@scikgu.kazan.su
authors.forename: I B
authors.initials: IB
authors.lastname: Leshchinskaya
authors.forename: E V
authors.initials: EV
authors.lastname: Shakirov
authors.forename: E L
authors.initials: EL
authors.lastname: Itskovitch
authors.forename: N P
authors.initials: NP
authors.lastname: Balaban
authors.forename: A M
authors.initials: AM
authors.lastname: Mardanova
authors.forename: M R
authors.initials: MR
authors.lastname: Sharipova
authors.forename: M B
authors.initials: MB
authors.lastname: Viryasov
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaya
authors.forename: V M
authors.initials: VM
authors.lastname: Stepanov
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004795:Enzyme Stability; D007531:Isoflurophate / Q000494:pharmacology; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013816:Thermodynamics
chemical_list: D007531:Isoflurophate; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase