Efficient gene expression in mammalian clock pacemaker cell in vitro by an adenovirus vector.
An efficient modified adeno system to express foreign genes to the central nervous system was developed recently. This modified recombinant adenoviral vectors can be used successfully to deliver lacZ to the hypothalamic suprachiasmatic nucleus (SCN) which is composed of mammalian clock pacemaker. The expression of lacZ in the primary culture of SCN was dose-dependent and higher enough in nearly 100% of these cells. We also showed that viral toxicity and lacZ overexpression had no serious effects on the rhythmic expression of arginine vasopressin (AVP) release from the SCN cell culture.
Yamazaki
N
N
Cell Biology Laboratory, National Institute of Bioscience and Human Technology (NIBH), AIST, MITI, Tsukuba Science City, Ibaraki, Japan.
Watanabe
K
K
Qu
Z
Z
Yamaoka
S
S
Ishida
N
N
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis / Q000235:genetics; D001683:Biological Clocks / Q000502:physiology*; D002478:Cells, Cultured; D015870:Gene Expression / Q000502:physiology; D005819:Genetic Markers; D005822:Genetic Vectors; D007763:Lac Operon / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000502:physiology*
D005819:Genetic Markers; D001127:Arginine Vasopressin