Cationic myeloma M-components frequently show cross-reacting anti-DNA, Anti-F(ab')2 and anti-nucleosome specificities.
134 cationic human IgG myeloma proteins were studied for expression of anti-DNA Idiotypic markers. 64 were studied for 16/6, F4, 3I, and 8.12, and 70 for expression of F4 and 3I. 31.3% showed at least one anti-DNA Id marker and many cationic myelomas were also positive for anti-DNA ELISA reactivity as well as anti-F(ab')2. Five M-components showed anti-nucleosome reactivity and one without detectable anti-DNA Id markers showed very strong anti-nucleosome antibody which was also inhibited by DNA and Sm antigens. Anti-idiotypic antisera produced either against Id(+) anti-DNA reactive M components or F(ab')2 fragments of affinity purified SLE IgG anti-DNA showed preferential cross-reactive idiotype reactivity between Id(+) anti-DNA reactive M components. Our findings indicate that human IgG monoclonal proteins positive for several common anti-DNA Ids and possessing anti-DNA ELISA reactivity, can serve as models for SLE Id marker antigens and as a source to prepare anti-Ids from IVIG.
Williams
R C
RC
Department of Medicine, University of Florida College of Medicine, Gainesville 32610, USA.
Malone
C C
CC
Silvestris
F
F
D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry; D000974:Antibodies, Antinuclear / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry / Q000276:immunology*; D002412:Cations; D003429:Cross Reactions; D004247:DNA / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D007130:Immunoglobulin Idiotypes / Q000737:chemistry; D007136:Immunoglobulins; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009707:Nucleosomes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D001798:Blood Proteins; D002412:Cations; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007136:Immunoglobulins; C009823:M-proteins (Myeloma); D009707:Nucleosomes; D004247:DNA