Suppression of L-histidine decarboxylase mRNA expression by methyleugenol.
We investigated the effect of methyleugenol on anaphylaxis. Methyleugenol completely inhibited systemic anaphylaxis induced by compound 48/80 in mice. Methyleugenol also inhibited local anaphylaxis activated by anti-dinitrophenyl (DNP) IgE. Moreover, methyleugenol dose-dependently inhibited histamine release in mast cells activated by compound 48/80 or anti-DNP IgE. Northern-blot analysis demonstrated that significantly reduced level of the mRNA of L-histidine decarboxylase (HDC) was expressed in mast cells treated with methyleugenol, compared to that without methyleugenol. We conclude that methyleugenol directly affect histamine release and HDC gene over-expression in mast cells.
Shin
B K
BK
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea.
Lee
E H
EH
Kim
H M
HM
D000818:Animals; D015152:Blotting, Northern; D005054:Eugenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006640:Histidine Decarboxylase / Q000235:genetics*; D008407:Mast Cells / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000494:pharmacology
D012333:RNA, Messenger; C005223:methyleugenol; D013373:Substance P; D005054:Eugenol; D006640:Histidine Decarboxylase