Fenofibrate differently alters expression of genes encoding ATP-binding transporter proteins of the peroxisomal membrane.
The 70-kDa peroxisomal membrane protein (PMP 70), adrenoleukodystrophy protein (ALDP) and adrenoleukodystrophy-related protein (ALDRP) belong to the ATP-binding transporter family, share a structure of half-transporters and are localized in the peroxisomal membrane of mammals. It was suggested that these proteins may heterodimerize to form functional transporters. The expression of the three genes was examined in various tissues of control or fenofibrate (a peroxisome proliferator)-treated rats using Northern and immuno-blotting techniques. The patterns of tissue expression were distinct for the three genes. Upon treatment, expression of the ALD gene was not altered while that of the PMP 70 and ALDR genes was strongly increased in intestine and liver, respectively. The absence of coordinated expression excludes that the three transporters function as exclusive and obligatory partners. We also report for the first time that the ALDR gene is inducible in rodents and that the corresponding mRNA is different in length in rat (3.0 and 5.5 kb) and in mouse and human (4.2 kb).
Albet
S
S
Laboratoire de Biologie Moléculaire et Cellulaire, Faculté des Sciences Mirande, Dijon, France.
Causeret
C
C
Bentejac
M
M
Mandel
J L
JL
Aubourg
P
P
Maurice
B
B
D000074061:ATP Binding Cassette Transporter, Subfamily D; D000074063:ATP Binding Cassette Transporter, Subfamily D, Member 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000818:Animals; D011345:Fenofibrate / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007425:Intracellular Membranes / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008830:Microbodies / Q000502:physiology*; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
C115572:ABCD1 protein, human; C431694:ABCD2 protein, human; D000074061:ATP Binding Cassette Transporter, Subfamily D; D000074063:ATP Binding Cassette Transporter, Subfamily D, Member 1; D018528:ATP-Binding Cassette Transporters; C489271:Abcd3 protein, mouse; C489272:Abcd3 protein, rat; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; D011345:Fenofibrate