title: Human herpesvirus type 8 DNA sequences in biological samples of HIV-positive and negative individuals in Sicily.
abstract: OBJECTIVE
  To evaluate the circulation of a new human herpesvirus (HHV), HHV-8 or Kaposi's sarcoma (KS)-associated herpesvirus in a geographical area where a high incidence rate of classical KS was already present before the appearance of the AIDS epidemic.
  
  
  METHODS
  The study was carried out by analysing: (i) bioptic samples from classic, AIDS-associated KS, and controls; (ii) peripheral blood mononuclear cells (PBMC) from classic KS, HIV-positive subjects with and without KS and healthy HIV-negative individuals; (iii) semen samples from heterosexual HIV-positive and HIV-negative individuals affected or not by KS; and (iv) cervical swabs from HIV-negative healthy heterosexual females. All specimens were tested for the presence of HHV-8 DNA sequences by a two-step polymerase chain reaction.
  
  
  RESULTS
  Positive results were obtained in 90% of bioptic samples of classic KS and in 100% of AIDS-associated KS. Viral sequences were also present in 50% of PBMC of subjects with classic KS and AIDS-associated KS, in 10% of AIDS patients without the angiosarcoma and in 11% of healthy HIV-negative individuals. Finally, HHV-8 DNA was detected in 13% of semen of HIV-negative heterosexual individuals and in 10% of AIDS patients without KS. Both PBMC and ejaculates from the same individual gave positive results. No HHV-8 sequences were found in cervical swabs.
  
  
  CONCLUSIONS
  HHV-8 is widespread in the general population in Sicily since it was detected in PBMC and semen of heterosexual HIV-negative individuals and is not found only in high-risk groups. The viral load appears to be more elevated in a high-risk population and it may be ascribed to a viral reactivation. The higher incidence rates of KS in Sicily compared with northern Italy and other European countries might be related to the presence of HHV-8 in the general population.
authors.affiliation: Dipartimento di Igiene e Microbiologia G. D'Alessandro, Università degli Studi, Palermo, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Viviano
authors.forename: F
authors.initials: F
authors.lastname: Vitale
authors.forename: F
authors.initials: F
authors.lastname: Ajello
authors.forename: A M
authors.initials: AM
authors.lastname: Perna
authors.forename: M R
authors.initials: MR
authors.lastname: Villafrate
authors.forename: F
authors.initials: F
authors.lastname: Bonura
authors.forename: M
authors.initials: M
authors.lastname: Aricò
authors.forename: G
authors.initials: G
authors.lastname: Mazzola
authors.forename: N
authors.initials: N
authors.lastname: Romano
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000453:epidemiology / Q000821:virology*; D000328:Adult; D004279:DNA, Viral / Q000032:analysis; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D012802:Sicily / Q000453:epidemiology
chemical_list: D004279:DNA, Viral