The expression of insecticide resistance-related cytochrome P450 forms is regulated by molting hormone in Drosophila melanogaster.
The expression and enzymatic activities of insecticide resistance-related cytochrome P450B are increased by the treatment with 20-hydroxyecdysone (20HE) in D. melanogaster Oregon R flies. We have explored the role of this hormone in the maintenance of P450B basal expression. Arrest of ecdysone synthesis led to a decrease in CYP6A2 mRNA level, as well as in P450B expression and activities. This effect occurred both in insecticide susceptible (ecd1) and resistant (IRED) strains carrying the temperature-sensitive ecd mutation. The role of the 20HE in the regulation of cytochrome P450-mediated insecticide resistance has been proposed.
Spiegelman
V S
VS
Institute of Carcinogenesis, Cancer Research Center, AMS of Russia, Moscow. vspiegelman@sprd1.mdacc.tmc.edu
Fuchs
S Y
SY
Belitsky
G A
GA
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000187:drug effects; D004331:Drosophila melanogaster / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004441:Ecdysterone / Q000494:pharmacology*; D007305:Insecticide Resistance; D010641:Phenotype
D004441:Ecdysterone; D003577:Cytochrome P-450 Enzyme System