title: Two widely used anti-DR alpha monoclonal antibodies bind to an intracellular C-terminal epitope.
abstract: In this report we show that two widely-used monoclonal antibodies, TAL-1B5 and DA6.147, which react with the HLA-DR alpha chain on immunoblots, recognize the C-terminal intracellular tail of this HLA-DR subunit. We demonstrate that both MoAbs react with a synthetic peptide representing the intracellular C-terminal tail of the DR alpha chain and that mutant DR molecules lacking this part of the alpha chain lose reactivity with TAL-1B5 and DA6.147, both in Western blot analysis and in intracellular FACS staining.
authors.affiliation: Human Immunogenetics Group, Imperial Cancer Research Fund, London, United Kingdom.
authors.forename: U
authors.initials: U
authors.lastname: Grüneberg
authors.forename: T
authors.initials: T
authors.lastname: Rich
authors.forename: C
authors.initials: C
authors.lastname: Roucard
authors.forename: S
authors.initials: S
authors.lastname: Marieke van Ham
authors.forename: D
authors.initials: D
authors.lastname: Charron
authors.forename: J
authors.initials: J
authors.lastname: Trowsdale
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001666:Binding Sites, Antibody; D000939:Epitopes / Q000276:immunology* / Q000378:metabolism*; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007424:Intracellular Fluid / Q000276:immunology; D008969:Molecular Sequence Data; D011487:Protein Conformation
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006684:HLA-DR Antigens