Expression of Glycine max (soybean agglutinin) binding molecule in injured motoneurons and its specific localization in the extracellular matrix between injured neurons and microglia.
In order to identify expression of molecules that are secreted or translocated into extracellular regions after nerve injury, lectin histochemistry using 16 lectins was carried out. After unilateral hypoglossal nerve transection, only Glycin max (soybean agglutinin; SBA) staining was apparent in the injured hypoglossal nucleus. The SBA staining was observed clearly from 3 days after the operation, and the most intense staining was observed around postoperative day 5-7. Thereafter SBA staining gradually decreased to an undetectable level by 21 days postoperative. Electron microscopic evaluation of the staining revealed it to be mainly concentrated in the extracellular region between injured motoneurons and microglia. In addition SBA staining was also found in the Golgi apparatus of injured motoneurons. These data suggest that injured motoneurons synthesize molecules to which SBA could bind in response to nerve injury, and that this molecule is secreted into the gap between microglia and injured neurons. This molecule may function as an adhesion or signalling molecule between injured neurons and microglia.
Ohshige-Hayashi
Y
Y
Department of Anatomy, Asahikawa Medical College, Nishikagura, Japan.
Kiyama
H
H
D000818:Animals; D005109:Extracellular Matrix / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008297:Male; D017628:Microglia / Q000378:metabolism*; D009046:Motor Neurons / Q000378:metabolism*; D009416:Nerve Regeneration / Q000502:physiology*; D037121:Plant Lectins; D051381:Rats; D017208:Rats, Wistar; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins