Evaluation of protein A and protein G as indicator system in an ELISA for detecting antibodies in mink to Pseudomonas aeruginosa.
A modified, indirect enzyme-linked immunosorbent assay (ELISA) was developed and applied in the detection of mink antibodies to Pseudomonas aeruginosa. In this assay, peroxidase conjugated protein A and protein G were evaluated as indicator systems for detecting antigen-antibody complexes. It was found that protein A has a strong affinity for mink immunoglobulins. In contrast, protein G showed no such affinity. The affinity of protein A for mink immunoglobulins was further demonstrated by immunoprecipitation assays.
Rivera
E
E
National Veterinary Institute, Uppsala, Sweden.
Jackert-Jernberger
M
M
Mejerland
T
T
Karlsson
K A
KA
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000145:classification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D008297:Male; D008907:Mink / Q000276:immunology*; D011552:Pseudomonas Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000276:immunology*
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D013205:Staphylococcal Protein A