Urinary excretion of sulfated polysaccharides administered to Wistar rats suggests a renal permselectivity to these polymers based on molecular size.
Sulfated polysaccharides were administered to Wistar rats and their elimination from the blood as well as their urinary excretion were evaluated. Sulfated polysaccharides with differences in molecular mass, charge density and molecular structure were obtained from algae, marine invertebrates and vertebrates. A simple methodology based on the metachromatic property of these polysaccharides with 1,9-dimethylmethylene blue was used to estimate their concentration in urine and blood. Renal permselectivity to these macromolecules was based on molecular size, but the upper limit of molecular mass for excretion of a sulfated polysaccharide in urine varies among polymers with different structures. For dextran sulfates the upper limit is approximately 8 kDa. Chondroitin 4- and 6-sulfates were excreted as fragments of approximately 30 kDa, which is smaller than the injected polysaccharide. This suggests that they were degraded enzymatically in vivo. Large synthetic polymers (dextran sulfate > 8 kDa) were not excreted in urine, but slowly disappeared from the blood. Evaluation of their tissue distribution after intravenous administration indicated that these molecules are preferentially accumulated in the kidney.
Guimarães
M A
MA
Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Brazil.
Mourão
P A
PA
D000818:Animals; D002809:Chondroitin Sulfates / Q000493:pharmacokinetics; D016264:Dextran Sulfate / Q000493:pharmacokinetics; D005685:Galactans / Q000493:pharmacokinetics; D006025:Glycosaminoglycans / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D008970:Molecular Weight; D011134:Polysaccharides / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
D005685:Galactans; D006025:Glycosaminoglycans; D011134:Polysaccharides; D002809:Chondroitin Sulfates; D016264:Dextran Sulfate; C007789:fucoidan