title: Aging and protein oxidative damage.
abstract: The hypothesis that aging is associated with the accumulation of oxidative damage was tested in the adult male housefly by monitoring the loss of membrane protein -SH groups, and activities of glucose-6-phosphate dehydrogenase and alcohol dehydrogenase, the cytosolic enzymes that are particularly susceptible to oxidative damage. Membrane protein -SH content and activities of these enzymes decreased with age and were correlated with the life expectancy or physiological age rather than the chronological age of the flies. Because the experimentally-induced loss of membrane protein -SH groups has a demonstrable deleterious effect on a variety of cellular functions, such damage during aging can be hypothesized to contribute to the physiological attrition associated with senescence.
authors.affiliation: Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275, USA.
authors.forename: S
authors.initials: S
authors.lastname: Agarwal
authors.forename: R S
authors.initials: RS
authors.lastname: Sohal
mesh_terms: D000375:Aging / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006793:Houseflies / Q000528:radiation effects; D008017:Life Expectancy; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D008565:Membrane Proteins; D013438:Sulfhydryl Compounds; D000426:Alcohol Dehydrogenase; D005954:Glucosephosphate Dehydrogenase