Incidence of diploid and disomic sperm nuclei in 45 infertile men.
Sperm cells from 45 infertile patients were investigated for disomy rates of chromosomes 1, 7, 10, 17, X and Y as well as for diploidy by single- and double-target in-situ hybridization. The patients who attended the infertility clinic were aged 23-46 years. Semenograms showed that the patients had oligo-, astheno-, oligoastheno-, oligoterato-, oligoasthenoterato-, or asthenoteratozoospermia. The average disomy rates determined in the patients were similar for all chromosomes, ranging from 0.10% (chromosome Y) to 0.14% (chromosomes 10 and X). Diploidy was detected with a mean incidence of 0.1%. With the exception of two patients who exhibited significantly increased diploidy rates of 0.35 and 1.6%, neither disomy nor diploidy was increased in the group of infertile patients as compared to healthy, fertile males.
Guttenbach
M
M
Department of Human Genetics, University of Würzburg, Germany.
Martinez-Expósito
M J
MJ
Michelmann
H W
HW
Engel
W
W
Schmid
M
M
D000328:Adult; D002467:Cell Nucleus / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D015342:DNA Probes; D004171:Diploidy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D013094:Spermatozoa / Q000648:ultrastructure*
D015342:DNA Probes