title: Nucleotide and calcium-induced conformational changes in histone H1.
abstract: The principal constituents of chromatin, histone H1 (H1) and the nucleosome have essential roles in regulation of eukaryotic gene expression. However, mechanisms for the H1-dependent inactivation and for the ATP-dependent chromatin remodeling upon activation are largely unelucidated. Using circular dichroism (CD) analysis we show that ATP and other nucleotides and Ca2+ induce structural changes in H1. ATP and Ca2+ also induce changes when H1 is interacting with DNA, and the changes in H1 are accompanied by alterations in its DNA interaction. These results suggest that nucleotide and Ca2+ binding may be important for H1-mediated chromatin changes.
authors.affiliation: Department of Applied Cell and Molecular Biology, University of Umeå, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Tarkka
authors.forename: J
authors.initials: J
authors.lastname: Oikarinen
authors.forename: T
authors.initials: T
authors.lastname: Grundström
mesh_terms: D000227:Adenine Nucleotides / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D006657:Histones / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D011487:Protein Conformation; D017510:Protein Folding; D051381:Rats
chemical_list: D000227:Adenine Nucleotides; D006657:Histones; D010710:Phosphates; D004247:DNA; D002118:Calcium