Sequential arrangement, not transcriptional activity, determines conformational stability of nucleosomes.
Gel electrophoresis in urea gradient was applied to study the unfolding effect of increasing concentrations of urea on the nucleosome structure. We showed that conformational stability of nucleosomes is determined by nucleotide sequence but not by transcriptional activity of DNA in chromatin.
Belikov
S
S
W. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Papatsenko
D
D
Preobrazhenskaya
O
O
Sushkov
V
V
Karpov
V
V
D000818:Animals; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure*; D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D004330:Drosophila / Q000196:embryology; D004625:Embryo, Nonmammalian; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009691:Nucleic Acid Denaturation; D009707:Nucleosomes / Q000737:chemistry / Q000648:ultrastructure*; D014158:Transcription, Genetic; D014508:Urea / Q000494:pharmacology
D002843:Chromatin; D009707:Nucleosomes; D014508:Urea; D004247:DNA