Effects of endothelin-1 on aggregation of normal and uremic platelets.
Platelet aggregation was studied in the whole blood and platelet rich plasma (PRP) upon stimulation with various agonists (collagen- 0.5 microgram/ml in the whole blood and PRP, ADP 10 microM in the whole blood and 2 microM in PRP, ristocetin 0.75 mg/ml in the whole blood and 1.25 mg/ml in PRP and arachidonic acid (AA) 0.5 mM in the whole blood and PRP) following incubation with ET-1 at final concentrations of 0.1 microM and 0.2 microM for 1 and 5 min. Collagen- and ADP-induced platelet aggregation in PRP was found to be diminished following incubation with ET-1 for 1 and 5 min at the concentrations of 0.1 microM and 0.2 microM. Aggregation of platelets upon stimulation with AA and ristocetin remained unaltered following incubation with ET-1. In the whole blood ristocetin-induced platelet aggregation was significantly diminished following incubation with ET-1 for 5 min, whereas AA-induced platelet aggregation was not significantly changed following preincubation with ET-1. Our results suggest that platelets may possess ET-1 receptor(s) and that ET-1 may also interact with other blood cells.
Pawlak
K
K
Nephrology Department, Białystok Medical School, Poland.
Małyszko
J S
JS
Małyszko
J
J
Myśliwiec
M
M
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D016718:Arachidonic Acid / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D019332:Endothelin-1 / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D012310:Ristocetin / Q000494:pharmacology; D014511:Uremia / Q000097:blood*
D019332:Endothelin-1; D010975:Platelet Aggregation Inhibitors; D012310:Ristocetin; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; D003094:Collagen