title: Specific roles of alpha-toxin and beta-toxin during Staphylococcus aureus corneal infection.
abstract: Staphylococcus aureus corneal infection results in extensive inflammation and tissue damage. Our previous studies of bacterial mutants have demonstrated a role for alpha-toxin in corneal virulence. This study analyzes, by genetic rescue experiments, the virulence of mutants affecting alpha-toxin and beta-toxin activity and demonstrates the ocular toxicity of these purified staphylococcal proteins. Three types of isogenic mutants were analyzed: (i) mutants specifically deficient in alpha-toxin (Hla) or beta-toxin (Hlb), (ii) a mutant deficient in both Hla and Hlb, and (iii) a regulatory mutant, deficient in the accessory gene regulator (agr), that produces reduced quantities of multiple exoproteins, including alpha- and beta-toxins. Plasmids coding for Hla and Hlb (pDU1212 and pCU1hlb, respectively) were used to restore toxin activity to mutants specifically deficient in each of these toxins. Either corneas were injected intrastromally with logarithmic-phase S. aureus or purified alpha- or beta-toxins were administered to normal eyes. Ocular pathology was evaluated by slit lamp examination and myeloperoxidase activity of infiltrating polymorphonuclear leukocytes. Corneal homogenates were cultured to determine the CFU per cornea. Eyes infected with the wild-type strain developed significantly greater corneal damage than eyes infected with Agr-, Hlb-, or Hla- strains. Epithelial erosions produced by parent strains were not produced by Agr- or Hla- strains. Hlb+ strains, unlike Hlb- strains, caused scleral edema. Plasmid pDU1212 restored corneal virulence to strain DU1090 (Hla-), and plasmid pCU1hlb restored corneal virulence to strain DU5719 (Hlb-). Application of purified alpha-toxin produced corneal epithelial erosions and iritis, while application of beta-toxin caused scleral inflammation. These studies confirm the role of alpha-toxin as a major virulence factor during S. aureus keratitis and implicate beta-toxin, a mediator of edema, as a lesser contributor to ocular damage.
authors.affiliation: Department of Microbiology, Immunology, and Parasitology, Louisiana State University Medical Center, New Orleans 70112, USA. rocall@pop3.lsumc.edu
authors.forename: R J
authors.initials: RJ
authors.lastname: O'Callaghan
authors.forename: M C
authors.initials: MC
authors.lastname: Callegan
authors.forename: J M
authors.initials: JM
authors.lastname: Moreau
authors.forename: L C
authors.initials: LC
authors.lastname: Green
authors.forename: T J
authors.initials: TJ
authors.lastname: Foster
authors.forename: O M
authors.initials: OM
authors.lastname: Hartford
authors.forename: L S
authors.initials: LS
authors.lastname: Engel
authors.forename: J M
authors.initials: JM
authors.lastname: Hill
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D015169:Colony Count, Microbial; D004487:Edema / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005123:Eye / Q000473:pathology; D005816:Genetic Complementation Test; D007500:Iritis / Q000382:microbiology; D007634:Keratitis / Q000382:microbiology*; D009504:Neutrophils / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism; D010957:Plasmids; D011817:Rabbits; D011995:Recombination, Genetic; D012590:Sclera / Q000473:pathology; D015423:Scleritis / Q000382:microbiology; D013203:Staphylococcal Infections / Q000235:genetics* / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000472:pathogenicity*; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: C095698:Agr protein, Staphylococcus aureus; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015534:Trans-Activators; D014157:Transcription Factors; D009195:Peroxidase