Characterization of a calcitonin gene related peptide-like molecule in the abalone, Haliotis tuberculata.
Immunoreactive related CGRP molecules (ir-CGRP) were identified in the abalone, Haliotis tuberculata, mainly in mantle and cephalic part extracts. Ir-CGRP in both tissues accounted for 461 and 455.6 pg per mg of proteins, respectively. These CGRP-immunoreactive molecules were further analyzed for their ability to interact with the CGRP radioreceptor assay. In specific target tissues for CGRP (rat liver membranes), 50% inhibition of 125I-labeled CGRP specific binding was observed with 4.7 micrograms and 21.1 micrograms of proteins from mantle and cephalic part extract, respectively. These molecules were submitted to gel-filtration chromatography on a Sephacryl S-100 column and were further analyzed in the radioreceptor assay specific for CGRP. The elution position of these molecules suggested a molecular weight close to that of synthetic salmon calcitonin.
Duvail
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L
Station de Biologie Marine du Muséum National d'Histoire Naturelle et du Collège de France, Concarneau, France.
Lopez
E
E
Fouchereau-Peron
M
M
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002850:Chromatography, Gel; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008099:Liver / Q000378:metabolism; D008974:Mollusca / Q000502:physiology*; D011506:Proteins / Q000032:analysis; D011863:Radioimmunoassay; D011869:Radioligand Assay; D051381:Rats
D007457:Iodine Radioisotopes; D011506:Proteins; D015740:Calcitonin Gene-Related Peptide