title: Flow-injection analysis of cobalt(II) utilizing enhanced lophine chemiluminescence with hydroxylammonium chloride.
abstract: A rapid and convenient flow-injection method is described for the determination of Co(II). The method utilized the phenomenon that the lophine-Co(II)-H2O2 chemiluminescence (CL) reaction is enhanced in alkaline media by the addition of hydroxylammonium chloride. The calibration curve was linear over the range 2 x 10(-7) to 2 x 10(-5) M with a detection limit of 4.5 x 10(-8) M (0.9 pmol) at a signal-to-noise ratio of 2. The method was applied to the assay of cyanocobalamin in commercially available eye lotions.
authors.affiliation: School of Pharmaceutical Sciences, Nagasaki University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
authors.forename: H
authors.initials: H
authors.lastname: Yamasaki
authors.forename: R
authors.initials: R
authors.lastname: Shimoda
authors.forename: N
authors.initials: N
authors.lastname: Kuroda
authors.forename: S
authors.initials: S
authors.lastname: Akiyama
authors.forename: W R
authors.initials: WR
authors.lastname: Baeyens
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D003035:Cobalt / Q000032:analysis*; D005123:Eye; D017022:Flow Injection Analysis / Q000379:methods*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000737:chemistry; D007093:Imidazoles; D008163:Luminescent Measurements; D009824:Ointments / Q000737:chemistry; D009883:Ophthalmic Solutions / Q000737:chemistry; D014805:Vitamin B 12 / Q000032:analysis
chemical_list: D006898:Hydroxylamines; D007093:Imidazoles; D009824:Ointments; D009883:Ophthalmic Solutions; D019811:Hydroxylamine; D003035:Cobalt; D014805:Vitamin B 12; C107378:lophine