Acyl-CoA:lysophosphatidylcholine acyltransferase activity in bovine retina rod outer segments.
In the present paper the properties of acyl-CoA:lysophosphatidylcholine acyltransferase activity associated with rod outer segments (ROS) have been studied. Under adequate experimental conditions, ROS acyl-CoA:lysophosphatidylcholine acyltransferase activity presented a maximum at pH 7.0. The enzyme was able to incorporate as much as 60% of the label offered as [1-14C]oleoyl-CoA into phosphatidylcholine after 5 min of incubation. The use of varying concentrations of oleoyl-CoA and 46 microM lysophosphatidylcholine gave an apparent K(m) value for oleoyl-CoA of 100 microM and a Vmax value of 153 nmol x h-1 x (mg protein)-1. The use of varying concentrations of lysophosphatidylcholine and 100 microM oleoyl-CoA gave an apparent K(m) value for lysophosphatidylcholine of 27 microM and a Vmax value of 155 nmol x h-1 x (mg protein)-1. The enzyme was inhibited by 25% when ROS membranes were incubated in the presence of 10 mM MgCl2. The acyltransferase was able to incorporate other acyl-CoAs (palmitoyl-CoA and arachidonoyl-CoA) into ROS phospholipids and to acylate other lysophospholipids but less efficiently than lysophosphatidylcholine. Lysophoshatidylcholine was preferentially acylated with arachidonic acid followed by oleic acid and, less efficiently, with palmitic acid. The high specific activity of acyl-CoA lysophosphatidylcholine acyltransferase found in purified ROS compared to the activity found in other subcellular fractions of the bovine retina suggests that this enzymatic activity is native to the ROS.
Castagnet
P I
PI
Instituto de Investigaciones Bioquímicas, Universidad Nacional del Sur y Consejo Nacional de Investigaciones Científicas y Técnicas, Bahía Blanca, Argentina.
Giusto
N M
NM
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002451:Cell Compartmentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity
D008244:Lysophosphatidylcholines; D010716:Phosphatidylinositols; D008243:1-Acylglycerophosphocholine O-Acyltransferase