The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
The enzymes in the dolichol pathway are membrane-proteins that utilize a combination of hydrophilic and extremely hydrophobic substrates. The enzymes in this pathway that have been purified and characterized to any extent have either been shown to be stabilized by mixed phospholipid/detergent micelles, or else require a lipid matrix for catalytic activity. Further understanding of the mechanisms of these essential enzymes may require developing methods for the reconstitution of the glycosyltransferases and their hydrophobic substrates in appropriate lipid matrices.
Schutzbach
J S
JS
The University of Alabama at Birmingham, Department of Microbiology, 35294-0019, USA.
D000818:Animals; D002240:Carbohydrate Sequence; D004286:Dolichols / Q000737:chemistry / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism; D006602:Hexosyltransferases; D006801:Humans; D050356:Lipid Metabolism; D008364:Mannosyltransferases / Q000378:metabolism*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D014166:Transferases / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups) / Q000378:metabolism
D004286:Dolichols; D008565:Membrane Proteins; D009844:Oligosaccharides; D014166:Transferases; D005964:Glucosyltransferases; D006602:Hexosyltransferases; D008364:Mannosyltransferases; C033917:UDPglucose dolicholphosphate glucosyltransferase; C033143:glycolipid 3-alpha-mannosyltransferase; C022755:dolichyl-phosphate beta-D-mannosyltransferase; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase; D017855:Transferases (Other Substituted Phosphate Groups); C023222:UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase