Molecular cloning of murine ICA69: diabetes-prone mice recognize the human autoimmune-epitope, Tep69, conserved in splice variants from both species.
The islet cell antigen ICA69 is an autoimmune target in most patients with insulin-dependent diabetes. Understanding its role in diabetic autoimmunity would be facilitated by an animal model. We therefore cloned mouse ICA69. The different splice variants now identified conserve Tep69, the single T cell epitope recognized by patient T cells. We show that diabetes-prone NOD mice had Tep69-specific, autoreactive T cell repertoires and thus provide a relevant model for the study of ICA69's role in diabetic autoimmunity.
Karges
W
W
The Hospital for Sick Children, University of Toronto, Department of Pediatrics, Ontario, Canada.
Gaedigk
R
R
Hui
M F
MF
Cheung
R
R
Dosch
H M
HM
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution
D001324:Autoantigens; D018076:DNA, Complementary; C087054:ICA1 protein, human; C490184:Ica1 protein, mouse; D008565:Membrane Proteins; D012333:RNA, Messenger