title: Tissue reactions to bacteria-inoculated rat lead samples. I. Effect of local gentamicin release through vicinal sponge or solution-dipping.
abstract: The effect of local gentamicin release through a vicinal collagen sponge or through preoperative solution-dipping of rat lead samples was investigated in an early-infection model. The efficacy of these methods and their effect on tissue response were determined. It was demonstrated that both methods of local gentamicin release suppress lead-related infectious complications as compared to the control lead, which showed a high presence of inflamed/infected tissues and bacterial growth at each explantation time point. The first day the vicinal collagen sponge was more effective in suppressing the infection than was the solution-dipped lead, probably because there is a faster and higher dose release of gentamicin from the sponge. However, continued implantation time revealed that gentamicin release from the solution-dipped lead was more effective than the sponge. This supports our hypothesis that the presence of lumina are decisive for bacterial growth and persistence of implant-related infections.
authors.affiliation: Groningen University, Laboratory for Cell Biology and Electron Microscopy, The Netherlands.
authors.forename: P B
authors.initials: PB
authors.lastname: van Wachem
authors.forename: M J
authors.initials: MJ
authors.lastname: van Luyn
authors.forename: A W
authors.initials: AW
authors.lastname: de Wit
authors.forename: D
authors.initials: D
authors.lastname: Raatjes
authors.forename: M
authors.initials: M
authors.lastname: Hendriks
authors.forename: M L
authors.initials: ML
authors.lastname: Verhoeven
authors.forename: P T
authors.initials: PT
authors.lastname: Cahalan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use*; D015169:Colony Count, Microbial; D004343:Drug Implants; D004567:Electrodes, Implanted; D005839:Gentamicins / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use*; D008297:Male; D016459:Prosthesis-Related Infections / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D004343:Drug Implants; D005839:Gentamicins