Evaluation of frog gastric mucous secretion by 7T magnetic resonance imaging.
This work describes a new method to evaluate the action of drugs on gastric mucus secretion by 7 T MRI. A polyethylene probe was inserted into the stomach of frogs, a water washing cycle was performed, air was inflated into the stomach, and the probe was removed. Drugs were administered topically by mixing them with the water used for stomach washing, and a series of images was collected. In untreated animals, a mucous layer emitting a high intensity signal covered the gastric mucosa. Atropine administration strongly reduced the mucous layer thickness. After pilocarpine administration, MRI images showed that the mucous layer was thicker than in controls. After metacholine administration, the thickness of the mucous layer was increased in comparison with both controls and pilocarpine-treated animals, in accord with the expected drug-induced increase of mucous secretion. Quantitative analysis of the mucous layer thickness on MRI images confirmed the above findings. In conclusion, these results indicate that MRI at 7 T is a sensitive in vivo screening test to evaluate the effect of drugs on gastric mucus secretion in absence of surgical manipulation, thereby enabling morphofunctional studies that cannot be performed by conventional methods.
Sbarbati
A
A
Istituto di Anatomia Umana ed Istologia, University of Verona, Italy.
Leclercq
F
F
Zancanaro
C
C
Guerrini
U
U
Antonakis
K
K
Osculati
F
F
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D018680:Cholinergic Antagonists / Q000494:pharmacology; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D009093:Mucus / Q000187:drug effects / Q000378:metabolism*; D010862:Pilocarpine / Q000494:pharmacology; D011893:Rana esculenta
D018679:Cholinergic Agonists; D018680:Cholinergic Antagonists; D010862:Pilocarpine; D016210:Methacholine Chloride; D001285:Atropine