Neuropeptide Y elevates intracellular Ca2+ and evokes noradrenaline release in SH-SY5Y cells.
Exposure of human neuroblastoma SH-SY5Y cells to 300 nM neuropeptide Y (NPY) or 1 microM muscarine separately failed to evoke release of [3H]noradrenaline ([3H]NA). Both agonists, however, induced a modest rise in [Ca2+]i. When NPY and muscarine were applied simultaneously, the rise in [Ca2+]i was greater than the sum of the rises of either agonist applied alone, and also evoked [3H]NA release, NPY evoked a rise in [Ca2+]i when applied during prolonged exposure to muscarine, although the peak level of [Ca2+]i was significantly lower (p < 0.05) than that reached following simultaneous application, and [3H]NA release was not stimulated. Simultaneous exposure of SH-SY5Y cells to muscarine and NPY thus induces a greater than additive rise in [Ca2+]i exceeding a critical level required to evoke [3H]NA release.
McDonald
R L
RL
Institute for Cardiovascular Research, University of Leeds, UK.
Vaughan
P F
PF
Peers
C
C
D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D009116:Muscarine / Q000494:pharmacology; D018721:Muscarinic Agonists / Q000494:pharmacology; D009478:Neuropeptide Y / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism*; D013268:Stimulation, Chemical
D018721:Muscarinic Agonists; D009478:Neuropeptide Y; D009116:Muscarine; D002118:Calcium; D009638:Norepinephrine