Can active immunization redirect an anti-IgE immune response?
We used as a template a mouse monoclonal antibody against IgE to isolate peptides from random peptide phage display libraries. Thereby, two types of peptides were isolated that corresponded to two different epitopes on the human IgE molecule. These peptides, also called mimotopes, seem to be a suitable tool in conjunction with carriers to induce an autoimmune response with a beneficial effect in humans, because the originally used template antibody is capable of neutralizing IgE, is nonanaphylactogenic, and inhibits IgE synthesis. The vaccination approach is further supported by the fact that we were capable of isolating anti-idiotypic antibodies from antibody phage display libraries against the template antibody. These anti-idiotypic antibodies were inhibited by both of the isolated IgE mimotopes. Thus, active vaccination with defined IgE mimotopes may represent a follow-up drug for the presently used anti-IgE antibodies.
Stadler
B M
BM
Institute of Immunology and Allergology, Inselspital, Bern, Switzerland. stadler@insel.unibe.ch
Rudolf
M P
MP
Vogel
M
M
Miescher
S
S
Zürcher
A W
AW
Kricek
F
F
D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis*; D006801:Humans; D014611:Vaccination
D000888:Antibodies, Anti-Idiotypic; C026408:anti-IgE antibodies