title: Dual infection of rabbits with human T cell lymphotropic virus types I and II.
abstract: Attempts were made to generate a rabbit model of dual infection with human T lymphotropic virus (HTLV) types I and II. Four groups (A, B, C, and D) of three rabbits each were used. Group A was inoculated with the RW-1 cell line coinfected with HTLV-I and HTLV-II and group B was transfused from a dually infected rabbit. Polymerase chain reaction (PCR) using primers specific for the pol region of each virus detected both HTLV-I and HTLV-II in all group A and two group B rabbits, but HTLV-II only in the remaining group B rabbit. Groups C and D already infected with HTLV-I and HTLV-II, respectively, were inoculated with an HTLV-II- or HTLV-I-producing cell line. One group C rabbit became PCR-positive for both viruses but the other five resisted superinfection with the respective viruses. During prolonged observation, three of the six dually infected rabbits converted to single (HTLV-I or HTLV-II) infection. The in vivo dual infection was confirmed by in vitro establishment of a lymphoid cell line coinfected with HTLV-I and HTLV-II. It was also possible to establish coinfected lymphoid cell lines from HTLV-I-infected rabbits by coculture with lethally irradiated HTLV-II-producing cells and vice versa. The mechanism of viral elimination in dually infected rabbits, as well as that of protective immunity against superinfection, remains to be elucidated.
authors.affiliation: Department of Medicine, Kochi Medical School, Nankoku.
authors.forename: H
authors.initials: H
authors.lastname: Machida
authors.forename: N
authors.initials: N
authors.lastname: Morishita
authors.forename: T
authors.initials: T
authors.lastname: Ikezoe
authors.forename: T
authors.initials: T
authors.lastname: Sawada
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: I
authors.initials: I
authors.lastname: Miyoshi
mesh_terms: D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005260:Female; D015490:HTLV-I Infections / Q000097:blood / Q000150:complications / Q000635:transmission*; D015491:HTLV-II Infections / Q000097:blood / Q000150:complications / Q000635:transmission*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification; D015367:Human T-lymphotropic virus 2 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011817:Rabbits; D015163:Superinfection / Q000097:blood / Q000635:transmission*