title: CD3- CD56+ non-Hodgkin's lymphomas with an aggressive behavior related to multidrug resistance.
abstract: CD56 expression has been reported previously in some non-Hodgkin's lymphoma (NHL) characterization. They principally involve the nasopharynx, are related to Epstein-Barr virus (EBV), and may be classified as either T- or non-T-natural killer (NK) cells according to CD3/T-cell receptor (TCR) status at the genomic or protein level. The present study reports three cases of non-nasal NK-NHL with the following characteristics: an agressive clinical behavior, heterogenous morphological data evoking pleomorphic T-cell malignant lymphoma, a non-T-NK phenotype using flow cytometry, and immunochemistry. The three cases were CD56+ without membrane expression of specific T markers (CD3, CD5, and TCR). Heterogenous results were observed concerning different antigens: CD2, CD4, CD8, CD16, CD94, CD122, TiA1, perforin, and granzyme B. There was no evidence of detectable clonal TCR gene rearrangement with polymerase chain reaction. No NK activity was detected in the two tested cases, and no relation was found with EBV. Multidrug resistance investigations suggest that agressive clinical findings could be related to MDR1 gene expression as confirmed by MDR1 mRNA detection, MDR1 gene product (Pgp) expression, and a functional multidrug resistance study using rhodamine efflux by flow-cytometry.
authors.affiliation: Laboratoire d'Hématologie-Immunologie, Hôpital Pontchaillou, CHRU Rennes, France.
authors.forename: B
authors.initials: B
authors.lastname: Drénou
authors.forename: T
authors.initials: T
authors.lastname: Lamy
authors.forename: L
authors.initials: L
authors.lastname: Amiot
authors.forename: O
authors.initials: O
authors.lastname: Fardel
authors.forename: S
authors.initials: S
authors.lastname: Caulet-Maugendre
authors.forename: M
authors.initials: M
authors.lastname: Sasportes
authors.forename: J
authors.initials: J
authors.lastname: Diebold
authors.forename: P Y
authors.initials: PY
authors.lastname: Le Prisé
authors.forename: R
authors.initials: R
authors.lastname: Fauchet
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis*; D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D016026:Bone Marrow Transplantation; D017252:CD3 Complex / Q000032:analysis*; D019002:CD56 Antigen / Q000032:analysis*; D002467:Cell Nucleus / Q000648:ultrastructure; D002945:Cisplatin / Q000008:administration & dosage; D003131:Combined Modality Therapy; D003520:Cyclophosphamide / Q000008:administration & dosage; D003561:Cytarabine / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005047:Etoposide / Q000008:administration & dosage; D017809:Fatal Outcome; D005260:Female; D005266:Femoral Neoplasms / Q000188:drug therapy / Q000473:pathology; D004854:Herpesvirus 4, Human; D006801:Humans; D015255:Idarubicin / Q000008:administration & dosage; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000187:drug effects*; D016400:Lymphoma, Large-Cell, Immunoblastic / Q000188:drug therapy / Q000473:pathology / Q000628:therapy; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000188:drug therapy / Q000473:pathology; D008297:Male; D008775:Methylprednisolone / Q000008:administration & dosage; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis*; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D010386:Pelvic Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000628:therapy; D011241:Prednisone / Q000008:administration & dosage; D011803:Quinine / Q000008:administration & dosage; D014750:Vincristine / Q000008:administration & dosage
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D017252:CD3 Complex; D019002:CD56 Antigen; D009363:Neoplasm Proteins; D003561:Cytarabine; D014750:Vincristine; D005047:Etoposide; D004317:Doxorubicin; D003520:Cyclophosphamide; D011803:Quinine; D002945:Cisplatin; D011241:Prednisone; D008775:Methylprednisolone; D015255:Idarubicin