title: Molecular characterisation of type 1 polioviruses associated with epidemics in South Africa.
abstract: The molecular epidemiology of wild-type 1 polioviruses isolated in South Africa during 2 major poliomyelitis epidemics in the 1980s and during the pre- and inter-epidemic periods was investigated by partial sequence analysis across the VP1/2A junction. Poliovirus-specific primers were used to amplify and subsequently sequence the region of interest. Viruses belonging to different genotypes were found to have been responsible for the 2 outbreaks. The Gazankulu outbreak in 1982 was caused by a poliovirus genotype which was unique to South Africa and which circulated endemically throughout much of the country between 1980 and 1985. Two additional genotypes, imported from the Middle East and West Africa, cocirculated endemically with the South African genotype between 1982 and 1985. The 1988 epidemic in Kwazulu-Natal was attributed to an imported genotype apparently introduced into South Africa in 1985 from countries north of the border. This genotype displaced the 3 genotypes previously in circulation and continued to be transmitted within the country until 1989, when the last confirmed cases of poliomyelitis associated with wild-type viruses were documented. All circulating wild-type poliovirus strains appear to have been eliminated from South Africa.
authors.affiliation: Department of Virology, University of the Witwatersrand Medical School, Johannesburg, South Africa.
authors.forename: C
authors.initials: C
authors.lastname: Chezzi
authors.forename: N K
authors.initials: NK
authors.lastname: Blackburn
authors.forename: B D
authors.initials: BD
authors.lastname: Schoub
mesh_terms: D004196:Disease Outbreaks; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D011051:Poliomyelitis / Q000453:epidemiology* / Q000235:genetics / Q000821:virology*; D017955:Poliovirus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013019:South Africa / Q000453:epidemiology