title: The Drosophila islet gene governs axon pathfinding and neurotransmitter identity.
abstract: We have isolated the Drosophila homolog of the vertebrate islet-1 and islet-2 genes, two members of the LIM homeodomain family implicated in the transcriptional control of motor neuronal differentiation. Similar to vertebrates, Drosophila islet is expressed in a discrete subset of embryonic motor neurons and interneurons that includes the dopaminergic and serotonergic cells of the ventral nerve cord. In contrast to mouse where mutation of islet-1 leads to loss of neurons due to programmed cell death, Drosophila islet is not required for neuron survival. Instead, loss of islet function causes defects in axon pathfinding and targeting plus loss of dopamine and serotonin synthesis. Ectopic expression of islet induces both specific alterations in pathfinding and changes in neurotransmitter identity. These findings indicate that islet coordinately controls two distinct aspects of neuronal identity.
authors.affiliation: Molecular Neurobiology Laboratory, The Salk Institute, San Diego, California 92186, USA.
authors.forename: S
authors.initials: S
authors.lastname: Thor
authors.forename: J B
authors.initials: JB
authors.lastname: Thomas
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001369:Axons / Q000502:physiology*; D002454:Cell Differentiation; D002465:Cell Movement; D004298:Dopamine / Q000378:metabolism; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D007395:Interneurons / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009046:Motor Neurons / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009420:Nervous System / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013045:Species Specificity; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014714:Vertebrates / Q000196:embryology / Q000235:genetics / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; D019476:Insect Proteins; C091135:Isl-2 protein, vertebrate; C558561:Isl2 protein, mouse; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; D012701:Serotonin; D004298:Dopamine