1,3,5-Trinitrobenzene-induced alpha-2u-globulin nephropathy.
Male and female Fischer-344 (F-344) and male NCI-Black-Reiter (NBR) rats were dosed with 0, 35.5, or 71 mg 1,3,5-trinitrobenzene (TNB)/kg/day for 10 days. Male F-344 rats were dosed with TNB (0 and 35.5 mg/kg) for 20 and 30 days. Hematoxylin and eosin and Mallory-Heidenhain stains and alpha-2u-globulin and proliferating cell nuclear antigen immunohistochemical stains were performed on kidney sections. All treated male F-344 rats exhibited dose-related accumulation of hyaline droplets containing alpha-2u-globulin in proximal tubules. The kidney weights were significantly increased in male and female rats treated with TNB. Significant increases in cell proliferation in proximal tubules were observed in male F-344 rats. Renal changes observed in TNB-treated rats appeared identical to those from other chemicals that induce alpha-2u-globulin nephropathy in male rats. No hyaline droplet accumulation was found in female F-344 and male NBR rats at any doses. We can conclude that TNB induces dose-related exacerbation of hyaline droplets containing alpha-2u-globulin in male rat kidney and subsequent cell proliferation.
Kim
S
S
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA.
Qualls
C W
CW
Reddy
G
G
Stair
E L
EL
D000510:Alpha-Globulins / Q000502:physiology*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006818:Hyalin / Q000737:chemistry; D007150:Immunohistochemistry; D007674:Kidney Diseases / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D014301:Trinitrobenzenes / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D000510:Alpha-Globulins; D018809:Proliferating Cell Nuclear Antigen; D014301:Trinitrobenzenes; D014874:Water Pollutants, Chemical; C013803:alpha 2u globulin; C029222:sym-trinitrobenzene