title: Bioavailability of vitamin E compounds and the effect of supplementation on release of superoxide and hydrogen peroxide by bovine neutrophils.
abstract: The effects of daily supplementation of 1000 IU of d-alpha- or dl-alpha-tocopherol acetate to Holstein cows from drying off to 8 wk after calving were studied 1) to compare the bioavailability of d-alpha- and dl-alpha-tocopherol acetate by monitoring vitamin E concentrations in plasma, red blood cells, and neutrophils and 2) to determine the effect of d-alpha- and dl-alpha-tocopherol acetate supplementation on O2- and H2O2 release by neutrophils. Supplementation with d-alpha- or dl-alpha-tocopherol acetate increased vitamin E concentrations in blood plasma, red blood cells, and neutrophils; the d-alpha form was more effective than was the dl-alpha form. Supplementation with d-alpha- or dl-alpha-tocopherol acetate had no effect on the release of O2- and H2O2 by neutrophils, suggesting no harmful effects on surrounding tissues from oxygen-free radicals produced by neutrophils.
authors.affiliation: Centre for Food and Animal Research, Agriculture and Agri-Food, Canada, Ottawa, ON, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Hidiroglou
authors.forename: T R
authors.initials: TR
authors.lastname: Batra
authors.forename: X
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001682:Biological Availability; D002417:Cattle; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006861:Hydrogen Peroxide / Q000097:blood*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D013481:Superoxides / Q000097:blood*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000097:blood / Q000493:pharmacokinetics*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
chemical_list: D013481:Superoxides; D014810:Vitamin E; D006861:Hydrogen Peroxide; D024502:alpha-Tocopherol; D013755:Tetradecanoylphorbol Acetate; D024505:Tocopherols