Partial substitution of di- and tripeptides for native proteins in sea bass diet improves Dicentrarchus labrax larval development.
To determine whether incorporation of peptides into diets can improve larval development, sea bass (Dicentrarchus labrax) larvae were fed for 21 d one of three isonitrogenous, isoenergetic semipurified diets in which enzymatic hydrolysate (75% di- and tripeptides) of fish meal proteins was substituted for 0, 20 or 40% of native fish meal proteins. Growth and survival were significantly greater (P < 0.05) in larvae fed peptide diets compared to those fed only native protein, with the best performance exhibited by those fed the 20% level of peptides. Chymotrypsin activity was much higher in groups fed peptide diets compared to that fed all native protein (P < 0.001), indicating a greater proteolytic capacity of the pancreas. At the intestinal level, activities of the brush border enzymes, aminopeptidase, maltase and gamma-glutamyl transpeptidase, increased with age while the cytosolic enzyme, leu-ala peptidase, decreased with age (P < 0.001). These changes in enzymatic activities correspond to the normal development of intestinal digestion. This development occurred earlier in the group fed 20% peptide-substituted diet than in the two other groups. The better larval performances observed in groups fed diets containing peptides can be related to the enhanced proteolytic capacity of the pancreas and the earlier development of intestinal digestion.
Zambonino Infante
J L
JL
Unité Mixte de Nutrition des Poissons IFREMER-INRA, Plouzané, France.jlzambon@ifremer.fr
Cahu
C L
CL
Peres
A
A
D000626:Aminopeptidases / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D017756:Aquaculture; D001492:Bass / Q000254:growth & development*; D002918:Chymotrypsin / Q000378:metabolism; D004032:Diet; D004044:Dietary Proteins / Q000494:pharmacology*; D004151:Dipeptides / Q000008:administration & dosage / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D009842:Oligopeptides / Q000008:administration & dosage / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism
D004044:Dietary Proteins; D004151:Dipeptides; D009842:Oligopeptides; D011492:Protein Hydrolysates; D000626:Aminopeptidases; D002918:Chymotrypsin; D014357:Trypsin