title: Identification, characterization, and developmental regulation of Chlamydia trachomatis 3-deoxy-D-manno-octulosonate (KDO)-8-phosphate synthetase and CMP-KDO synthetase.
abstract: The kdsA and kdsB genes from Chlamydia trachomatis encoding 3-deoxy-D-manno-octulosonate (KDO)-8-phosphate synthetase and CMP-KDO synthetase were identified by functional complementation of temperature-sensitive Salmonella typhimurium mutants, homology to known KDO-8-phosphate synthetase and CMP-KDO synthetase proteins, and in vitro enzyme activity. The kdsA gene was transcribed as part of a polycistronic mRNA with two downstream open reading frames (ORFs). One of these ORFs appeared to encode a membrane-anchored protein, while the second encoded a protein showing homology to the ATP-binding component of periplasmic binding protein-dependent ABC transporters. Transcription of kdsA and kdsB in C. trachomatis was evident within 4 h of initiation of the C. trachomatis infection process and continued throughout the chlamydial life cycle.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
authors.forename: J L
authors.initials: JL
authors.lastname: Wylie
authors.forename: E R
authors.initials: ER
authors.lastname: Iliffe
authors.forename: L L
authors.initials: LL
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: McClarty
mesh_terms: D000446:Aldehyde-Lyases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: C020011:2-dehydro-3-deoxyphosphooctonate aldolase; D009713:Nucleotidyltransferases; C028984:3-deoxy-manno-octulosonate cytidyltransferase; D000446:Aldehyde-Lyases