Absorption profiles for polyethylene glycols after regional jejunal perfusion and oral load in healthy humans.
The study aimed at applying closed segmental intestinal perfusion with a multichannel tube (Loc-I-Gut) for studies of regional small bowel permeability to different-sized polyethylene glycols and to compare jejunal absorption with absorption after oral load in healthy subjects. Intestinal perfusion was performed in 10 healthy volunteers and recovery of (14)C-labeled polyethylene glycol 4000 was used to detect leakage. Absorption of polyethylene glycols 546-942 was measured as urinary recovery after perfusion and oral load, and as disappearance from the lumen during steady-state perfusion. Steady-state conditions were obtained during the second hour of perfusion. Absorption of polyethylene glycols during perfusion was size-selective for molecular weights 546-766, but not for 810-942. After oral load, absorption was size-selective throughout 546-942. Segmental perfusion with Loc-I-Gut may be used to study regional jejunal permeability. Our data support the hypothesis of a dual pore system for absorption of hydrophilic molecules in the human jejunum.
Söderholm
J D
JD
Department of Surgery, University Hospital, Linköping, Sweden.
Olaison
G
G
Kald
A
A
Tagesson
C
C
Sjödahl
R
R
D000284:Administration, Oral; D000328:Adult; D005260:Female; D006801:Humans; D007408:Intestinal Absorption; D007441:Intubation, Gastrointestinal; D007583:Jejunum / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D010477:Perfusion; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000493:pharmacokinetics
D011092:Polyethylene Glycols