A sensory axon repellent secreted from ventral spinal cord explants is neutralized by antibodies raised against collapsin-1.
During embryogenesis, different subclasses of sensory neurons extend central projections to specific locations in the spinal cord. Muscle and cutaneous afferents initially project to the same location in the dorsal cord. Later, specific muscle afferents leave other afferents behind and project into the ventral cord. Previous studies have shown that ventral spinal cord explants secrete a repellent for sensory neurites. We now find that antibodies to collapsin-1 neutralize this repellent activity. Additional data suggest that all afferents respond to collapsin-1 when they are first confined to the dorsal cord, but that ventrally projecting muscle afferents become collapsin-1 insensitive as they project into the ventral cord. Our results suggest that the transient dorsal expression of collapsin-1 prevents all efferents from entering the cord early and sustained ventral expression prevents dorsally terminating afferents from entering the ventral cord later.
Shepherd
I T
IT
Department of Neuroscience, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Luo
Y
Y
Lefcort
F
F
Reichardt
L F
LF
Raper
J A
JA
D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006131:Growth Inhibitors / Q000276:immunology / Q000378:metabolism*; D017403:In Situ Hybridization; D008959:Models, Neurological; D009132:Muscles / Q000294:innervation; D009414:Nerve Growth Factors / Q000276:immunology / Q000378:metabolism*; D009475:Neurons, Afferent / Q000187:drug effects*; D009500:Neutralization Tests; D012333:RNA, Messenger / Q000302:isolation & purification; D040121:Semaphorin-3A; D012867:Skin / Q000294:innervation; D013116:Spinal Cord / Q000196:embryology*
D006023:Glycoproteins; D006131:Growth Inhibitors; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D040121:Semaphorin-3A