Effects of K+ channel inhibitors on potassium transport in bovine adrenal chromaffin granules.
The Ca(2+)-independent K+ selective channel in the membrane of adrenal gland chromaffin granules has earlier been identified. In the present report we describe new properties of this potassium channel using 86Rb+, a K+ analogue, flux measurements. The studies are performed in membrane vesicles prepared from chromaffin granules. The electrogenic 86Rb+ transport is inhibited by quinacrine, barium, zinc and magnesium. The effects of other potassium channel blockers on 86Rb+ transport into chromaffin granules are also reported.
Lobanov
N A
NA
Institute of Bioorganic Chemistry, Belorussian Academy of Sciences, Minsk, Belarus.
Szewczyk
A
A
Wójcik
G
G
Nowotny
M
M
Nałecz
M J
MJ
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002413:Cations, Divalent / Q000494:pharmacology; D002417:Cattle; D002712:Chlorides / Q000378:metabolism*; D002837:Chromaffin Granules / Q000378:metabolism*; D003573:Cytochrome b Group / Q000032:analysis; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D026902:Potassium Channel Blockers; D011796:Quinacrine / Q000494:pharmacology; D012413:Rubidium / Q000378:metabolism*
D002413:Cations, Divalent; D002712:Chlorides; D003573:Cytochrome b Group; D026902:Potassium Channel Blockers; C023646:cytochrome b561; D011796:Quinacrine; D012413:Rubidium; C032710:rubidium chloride; D011188:Potassium