A hematopoietic cell L-selectin ligand exhibits sulfate-independent binding activity.
L-selectin is a leukocyte cell-surface glycoprotein that mediates adhesive interactions between circulating cells and vascular endothelium. All endothelial ligands of L-selectin characterized to date are glycoproteins that require sulfation for activity and share reactivity with MECA 79, a monoclonal antibody that recognizes a sulfate-dependent epitope involved in L-selectin attachment. We have recently identified by functional assay a glycoprotein L-selectin ligand expressed on the human hematopoietic cell line KG1a. We report here that this ligand is not recognized by MECA 79 and that it retains binding activity after metabolic inhibition of sulfation by chlorate. A native membrane L-selectin ligand exhibiting sulfate-independent function has not been described previously. Identification of this novel ligand on a nonendothelial cell type suggests that structural determinants conferring L-selectin binding may vary in a cell- and tissue-specific manner.
Sackstein
R
R
H. Lee Moffitt Cancer Center & Research Institute, University of South Florida College of Medicine, Tampa 33612, USA.
Fu
L
L
Allen
K L
KL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D002704:Chlorates / Q000494:pharmacology; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D019041:L-Selectin / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D011485:Protein Binding; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012794:Sialic Acids / Q000276:immunology / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002704:Chlorates; D000939:Epitopes; D008024:Ligands; D012794:Sialic Acids; D013431:Sulfates; D019041:L-Selectin; D009439:Neuraminidase; C032706:sodium chlorate