Differential expression of bcl-2 and bcl-x during chicken spermatogenesis.
We report the isolation and characterization of a chicken testis bcl-XL cDNA coding for a long bcl-x protein with a hydrophobic tail, and the expression of bcl-2 and bcl-x during chicken spermatogenesis. Bcl-2 is highly expressed in embryonic and immature testes enriched in spermatogonia and barely detectable in mature testes, where most of the cells are meiotic and postmeiotic. Bcl-x is expressed in both mature and immature testes, but in a lesser amount in mature testes. Differential expression of bcl-2 and bcl-x during spermatogenesis is consistent with the reported different susceptibility to apoptosis of spermatogonia, and meiotic and postmeiotic cells.
Vilagrasa
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X
Molecular Genetics Research Group, Faculty of Medicine, University of Barcelona, Spain.
Mezquita
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C
Mezquita
J
J
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001483:Base Sequence; D015152:Blotting, Northern; D002642:Chick Embryo; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000196:embryology / Q000378:metabolism*; D051020:bcl-X Protein
D017931:DNA Primers; D018076:DNA, Complementary; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein