title: Structural analysis of the O-antigenic polysaccharide from the enteropathogenic Escherichia coli O142.
abstract: The polysaccharide part of the lipopolysaccharide obtained from the enteropathogenic Escherichia coli O142 has been isolated, and its structure determined. Together with 1H-NMR and 13C-NMR spectroscopy, sugar and methylation analyses show that the polysaccharide is composed of repeating pentasaccharide units. Sequential information on the O-polysaccharide was obtained by two-dimensional NMR techniques, namely heteronuclear-multiple-bond-connectivity and NOESY experiments. The repeating unit of the O-polysaccharide of E. coli strain O142 has the following structure: [structure: see text].
authors.affiliation: Department of Organic Chemistry, Stockholm University, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Landersjö
authors.forename: A
authors.initials: A
authors.lastname: Weintraub
authors.forename: G
authors.initials: G
authors.lastname: Widmalm
mesh_terms: D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D003967:Diarrhea / Q000209:etiology; D004926:Escherichia coli / Q000737:chemistry* / Q000145:classification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000209:etiology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry*; D012703:Serotyping; D013045:Species Specificity; D014774:Virulence
chemical_list: D002241:Carbohydrates; D019081:O Antigens