Determination of trazodone and its metabolite, 1-m-chlorophenyl-piperazine, in human plasma and red blood cell samples by HPLC.
OBJECTIVE
To develop an HPLC method for the analysis of trazodone and its metabolite, 1-m-chlorophenyl-piperazine (m-CPP), in human plasma and red blood cells.


METHODS
The analytes were extracted by heptane containing 1.5% isoamyl alcohol, back extracted into phosphoric acid and analyzed by reverse phase HPLC with UV detection.


RESULTS
In seven randomly selected, male, human subjects plasma concentrations (nmol/l) were 380-5841 for trazodone and 14-237 for m-CPP while those in packed red blood cells were 98-634 for trazodone and 15-155 for m-CPP. Plasma trazodone concentrations were 4-11-fold higher than those in red blood cells while those of m-CPP were about equal. This may be the first report on concentrations of trazodone and m-CPP in human red blood cells.


CONCLUSIONS
This sensitive method can be used for monitoring trazodone and m-CPP in human plasma and red blood cells.
Vatassery
G T
GT
GRECC Program, Research Service, VA Medical Center, Minneapolis, MN 55417, USA.
Holden
L A
LA
Hazel
D K
DK
Dysken
M W
MW
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010879:Piperazines / Q000097:blood*; D017366:Serotonin Receptor Agonists / Q000097:blood*; D014196:Trazodone / Q000097:blood*
D010879:Piperazines; D017366:Serotonin Receptor Agonists; C015068:1-(3-chlorophenyl)piperazine; D014196:Trazodone