T cell lymphokines modulate bFGF-induced smooth muscle cell fibrinolysis and migration.
Smooth muscle cell (SMC) fibrinolysis is necessary for SMC migration. To determine whether the T cell lymphokines interleukin 4 (IL-4) and interferon-gamma (IFN-gamma) modulate SMC fibrinolysis and migration induced by basic fibroblast growth factor (bFGF), we examined the effects of IL-4 and IFN-gamma on human SMC tissue-type plasminogen activator (tPA), urokinase-type plasminogen activator (UPA), and plasminogen activator inhibitor 1 (PAI-1) antigen production, determined by enzyme-linked immunosorbent assays. Although IL-4 had no effects on SMC tPA, UPA, and PAI-1 production, it potentiated bFGF-induced tPA, UPA, and PAI-1 antigens. IL-4 plus bFGF resulted in a net increase in SMC fibrinolytic activity. IFN-gamma did not significantly affect bFGF induction of SMC tPA and PAI-1 antigens. However, IFN-gamma significantly decreased bFGF-mediated induction of SMC UPA antigen. IFN-gamma decreased the IL-4 plus bFGF induction of both tPA and UPA antigens. IL-4 increased and IFN-gamma abrogated bFGF induction of in vitro SMC migration through a modified micro-Boyden chamber. Therefore, IL-4 and IFN-gamma modulate bFGF-mediated induction of in vitro vascular SMC fibrinolysis and migration.
Wang
W
W
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Chen
H J
HJ
Schwartz
A
A
Cannon
P J
PJ
Rabbani
L E
LE
D000941:Antigens / Q000032:analysis; D002465:Cell Movement / Q000502:physiology; D005342:Fibrinolysis / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D008222:Lymphokines / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D017395:Plasminogen Activator Inhibitor 1 / Q000276:immunology; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000276:immunology; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology
D000941:Antigens; D008222:Lymphokines; D017395:Plasminogen Activator Inhibitor 1; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D015847:Interleukin-4; D007371:Interferon-gamma; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator