Identification, characterization, and developmental regulation of Chlamydia trachomatis 3-deoxy-D-manno-octulosonate (KDO)-8-phosphate synthetase and CMP-KDO synthetase.
The kdsA and kdsB genes from Chlamydia trachomatis encoding 3-deoxy-D-manno-octulosonate (KDO)-8-phosphate synthetase and CMP-KDO synthetase were identified by functional complementation of temperature-sensitive Salmonella typhimurium mutants, homology to known KDO-8-phosphate synthetase and CMP-KDO synthetase proteins, and in vitro enzyme activity. The kdsA gene was transcribed as part of a polycistronic mRNA with two downstream open reading frames (ORFs). One of these ORFs appeared to encode a membrane-anchored protein, while the second encoded a protein showing homology to the ATP-binding component of periplasmic binding protein-dependent ABC transporters. Transcription of kdsA and kdsB in C. trachomatis was evident within 4 h of initiation of the C. trachomatis infection process and continued throughout the chlamydial life cycle.
Wylie
J L
JL
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Iliffe
E R
ER
Wang
L L
LL
McClarty
G
G
D000446:Aldehyde-Lyases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D016415:Sequence Alignment
C020011:2-dehydro-3-deoxyphosphooctonate aldolase; D009713:Nucleotidyltransferases; C028984:3-deoxy-manno-octulosonate cytidyltransferase; D000446:Aldehyde-Lyases