Quantification of urinary uric acid in the presence of thymol and thimerosal by high-performance liquid chromatography.
A high-performance liquid chromatographic method was developed as an alternative to automated enzymatic analysis of uric acid in human urine preserved with thymol and/or thimerosal. Uric acid (tR = 10 min) and creatinine (tR = 5 min) were separated and quantified during isocratic elution (0.025 M acetate buffer, pH 4.5) from a mu Bondapak C18 column. The uric-acid peak was identified chemically by incubating urine samples with uricase. The thymol/thimerosal peak appeared at 31 min during the washing step and did not interfere with the analysis. We validated the high-performance liquid chromatographic method for linearity, precision and accuracy, and the results were found to be excellent.
Chen
Y
Y
KRUG Life Sciences Inc., Houston, TX 77058, USA.
Pietrzyk
R A
RA
Whitson
P A
PA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003404:Creatinine / Q000652:urine; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011310:Preservatives, Pharmaceutical; D012680:Sensitivity and Specificity; D013849:Thimerosal; D013943:Thymol; D014527:Uric Acid / Q000652:urine*
D011310:Preservatives, Pharmaceutical; D013849:Thimerosal; D014527:Uric Acid; D013943:Thymol; D003404:Creatinine