Two limonoids from Turraea obtusifolia (Meliaceae), prieurianin and rohitukin, antagonise 20-hydroxyecdysone action in a Drosophila cell line.
Bioassay-assisted HPLC analyses yielded two prieurianin-type limonoids, prieurianin and rohitukin, from the seeds of Turraea obtusifolia, which act as antagonists of 20-hydroxyecdysone action in the Drosophila melanogaster BII cell line. With a 20-hydroxyecdysone concentration of 5 x 10(-8) M, the ED50 values for prieurianin and rohitukin are 10(-5) M and 1.25 x 10(-4) M, respectively.
Sarker
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SD
Department of Biological Sciences, University of Exeter, Washington Singer Laboratories, Devon, UK.
Savchenko
T
T
Whiting
P
P
Sik
V
V
Dinan
L
L
D000818:Animals; D002460:Cell Line; D004331:Drosophila melanogaster; D004441:Ecdysterone / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D036701:Limonins; D015394:Molecular Structure; D010936:Plant Extracts; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D036701:Limonins; D010936:Plant Extracts; D014315:Triterpenes; D004441:Ecdysterone; C105838:prieurianin; C105839:rohitukin