title: Sperm membrane incorporation into oolemma contributes to the oolemma block to sperm penetration: evidence based on intracytoplasmic sperm injection experiments in the mouse.
abstract: Mouse oocytes were fertilized by intracytoplasmic sperm injection (ICSI) and reinseminated after the removal of zonae pellucidae at pronuclear stage or at the 2-cell stage. Although these oocytes were activated normally by ICSI, as evidenced by resumption of meiosis and cortical granule exocytosis, they did not develop oolemma block to sperm penetration. They could be penetrated by spermatozoa at pronuclear stage and even at the 2-cell stage. This supports the notion that incorporation of sperm plasma membrane into oolemma contributes to the changes in oolemma that block sperm penetration.
authors.affiliation: Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, USA.
authors.forename: M
authors.initials: M
authors.lastname: Maleszewski
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: R
authors.initials: R
authors.lastname: Yanagimachi
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008845:Microinjections; D010063:Ovum / Q000502:physiology*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*