Production of beta-mannanase by B. subtilis from agro-industrial by-products: screening and optimization. Mannanase from wastes.
We have demonstrated that a mixture of wheat bran (35 g l-1), as a main substrate, and palm seed powder (10 g l-1), as a co-substrate, is appropriate for beta-mannanase production by Bacillus subtilis. A 2n factorial experimental design was employed as a primary step for medium optimization. The enzyme activity titters obtained at the optimized growth condition were equivalent to about 319% of the beta-mannanase activity and 114% of the specific activity levels reached by a galactomannan-based culture.
el-Helow
E R
ER
Department of Botany & Microbiology, Faculty of Science, Alexandria, Egypt.
Sabry
S A
SA
Khattab
A A
AA
D000645:Ammonium Sulfate / Q000378:metabolism; D001412:Bacillus subtilis / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism*; D005690:Galactose / Q000031:analogs & derivatives; D007220:Industrial Waste; D008351:Mannans / Q000378:metabolism; D008361:Mannosidases / Q000096:biosynthesis*; D009566:Nitrates / Q000378:metabolism; D011208:Powders / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D014908:Triticum / Q000378:metabolism; D014866:Waste Products; D044902:beta-Mannosidase
D003470:Culture Media; D007220:Industrial Waste; D008351:Mannans; D009566:Nitrates; D011208:Powders; D014866:Waste Products; C012990:galactomannan; D008361:Mannosidases; D044902:beta-Mannosidase; D000645:Ammonium Sulfate; C006568:ammonium nitrate; D005690:Galactose