title: Large volume leukapheresis maximizes the progenitor cell yield for allogeneic peripheral blood progenitor donation.
abstract: We have investigated the efficiency and safety of large volume leukapheresis (LVL) for the collection of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood progenitor cells (PBPCs) from healthy donors. In six apheresis sessions in four healthy individuals on a COBE-BCT Spectra cell separator (median processed volume 3.5 X total blood volume, TBV, range 3.3-4.4 X TBV), harvested cells were collected sequentially into three single bags. The collection bags were changed after processing 33%, 66%, and 100% of the prospective apheresis volume, allowing analysis of PBPCs collected at different periods during one harvest. Mononuclear cells (MNCs), CD34+ cells, CD34+ subsets, and lymphocyte subsets were determined in each bag. Substantially more PBPCs were harvested than were in the circulation before G-CSF administration preceding LVL (median 171%, range 69-267%), reflecting progenitor release during the procedure. In donors 1 and 3, the CD34+ cell yields decreased in the third bag to 53% and 42% of that collected in the first bag, whereas the progenitor cell yields in donors 2 and 4 were stable or rose during the procedure, achieving in the third bag 157% and 105% of the number of CD34+ cells collected in the first bag. Minor changes were found in the subsets of CD34+ cells, lymphocytes, and monocytes collected at different periods during a single harvest. LVL was well tolerated. Reversible thombocytopenia developed in all cases. No late effects attributable to LVL or G-CSF were found in the 4 donors and 16 other healthy individuals who have undergone LVL in our institution. We conclude that LVL is safe and maximizes PBPC yields for allogeneic transplantation.
authors.affiliation: Department of Hematology, Heinrich Heine University Düsseldorf, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Kobbe
authors.forename: D
authors.initials: D
authors.lastname: Soehngen
authors.forename: A
authors.initials: A
authors.lastname: Heyll
authors.forename: J
authors.initials: J
authors.lastname: Fischer
authors.forename: K P
authors.initials: KP
authors.lastname: Thiele
authors.forename: C
authors.initials: C
authors.lastname: Aul
authors.forename: P
authors.initials: P
authors.lastname: Wernet
mesh_terms: D000328:Adult; D001782:Blood Donors; D002455:Cell Division; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006650:Histocompatibility Testing; D006801:Humans; D007937:Leukapheresis / Q000379:methods*; D007958:Leukocyte Count; D017708:Leukocyte Transfusion / Q000379:methods; D008297:Male
chemical_list: D016179:Granulocyte Colony-Stimulating Factor