Nitric oxide inhibits capping in HL-60 cells.
In the present study, we investigated the effect of nitric oxide (NO) on capping, which is associated with the actin polymerization in HL-60 cells (human promyelocytic leukemia cells). We first assessed the effect of NO on the patching and capping by using anti-human LFA-1 monoclonal antibody. Samples were analyzed by a fluorescence microscope. As expected, NO inhibited the percentage of capping dose dependently. We compared the effect of NO on capping with cytochalasin D (CD) and observed that CD also inhibits the capping in HL-60 cells. We next examined the effect of NO on the F-actin content. For assays of F-actin content, the FITC labelled phalloidin was permeabilized and stained in HL-60 cells. The bound fluorescence quantified by flow cytometry using a FACStar. There was a decrease in the F-actin formation in NO treated cells. Taken together, these data indicate that NO inhibits the capping on cellular membrane by decreasing the intracellular F-actin formation in HL-60 cells. We suggest that the formation of capping linked with actin polymerization at the inner leaflet of plasma membrane may be regulated by NO.
Lee
J H
JH
Department of Microbiology and Immunology, Wonkwang University School of Medicine, Chonbuk, Republic of Korea.
Ryu
H
H
Han
M K
MK
Kim
U H
UH
Chung
H T
HT
D000199:Actins / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000911:Antibodies, Monoclonal; D001704:Biopolymers / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D015638:Cytochalasin D / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007152:Immunologic Capping / Q000187:drug effects* / Q000502:physiology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology
D000199:Actins; D000911:Antibodies, Monoclonal; D001704:Biopolymers; D016169:Lymphocyte Function-Associated Antigen-1; D019384:Nucleic Acid Synthesis Inhibitors; D009599:Nitroprusside; D000246:Adenosine Diphosphate Ribose; D015638:Cytochalasin D; D009569:Nitric Oxide