title: The red deer (Cervus elaphus) contains two expressed major histocompatibility complex class II DQB genes.
abstract: The highly variable second exons of the red deer (Cervus elaphus) major histocompatibility complex (MHC) DQB genes were cloned and sequenced. Eight different expressed DQB sequences were isolated from four unrelated red deer. Either two or three different DQB sequences were isolated from each individual, demonstrating that more than one DQB gene is expressed in red deer. This is consistent with other ruminant, which also have multiple expressed copies of the DQB gene. The sequences ranged in similarity from 81.3% to 97.6% with an average similarity of 90.2%; this indicates that at least one of the DQB genes is highly polymorphic.
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: P A
authors.initials: PA
authors.lastname: Swarbrick
authors.forename: A M
authors.initials: AM
authors.lastname: Crawford
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003670:Deer / Q000235:genetics*; D005091:Exons / Q000235:genetics; D015870:Gene Expression; D005802:Genes, MHC Class II / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C057615:HLA-DQbeta antigen