Densitometric determination of metoprolol tartrate in pharmaceutical dosage forms.
This paper describes a simple densitometric method for the determination of metroprolol tartrate in tablets and ampoules. After separation on silica gel GF254 plates, using acetone-methanol-triethylamine as the mobile phase for the tablets and acetone-triethylamine for ampoules, the chromatographic zones corresponding to the spots of metoprolol were scanned. Quantitation was performed using a computer-controlled Camag TLC scanner and applying five-point calibration with polynomial regression. The calibration function was established in the ranges 1-28 micrograms for tablets and 1-9 micrograms for ampoules. The results obtained are precise and reproducible, with recovery values of 99.1-99.4%.
Vujic
Z
Z
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Belgrade, Yugoslavia.
Radulovic
D
D
Agbaba
D
D
D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage / Q000032:analysis*; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003720:Densitometry / Q000379:methods; D008790:Metoprolol / Q000008:administration & dosage / Q000032:analysis*; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D000319:Adrenergic beta-Antagonists; D013607:Tablets; D008790:Metoprolol