Assessment of PCB estrogenicity in a human breast cancer cell line.
Lower chlorinated, ortho substituted, non coplanar polychlorinated biphenyls (PCBs) are weakly estrogenic in rodents and in some in vitro assays. The estrogenic potency of six PCB congeners and one of their para-hydroxylated metabolites have been tested in an estrogen-responsive MCF-7 human breast-cancer cell-culture system, to evaluate the utility of this system for assessment of PCB and hydroxylated PCB estrogenic activity. This assay is based on the estrogen receptor-mediated induction of postconfluent cell proliferation. The results of the limited test series were generally consistent with, but not absolute in the requirement for ortho-chlorine substitution and para-hydroxylation for estrogenic potency, demonstrating the usefulness of the MCF-7 focus assay for estrogenic structure-activity evaluation of PCBs.
Gierthy
J F
JF
Wadsworth Center, New York State Department of Health, Albany 12201-0509, USA.
Arcaro
K F
KF
Floyd
M
M
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D004785:Environmental Pollutants / Q000633:toxicity*; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000633:toxicity*; D005069:Evaluation Studies as Topic; D006801:Humans; D006900:Hydroxylation; D016015:Logistic Models; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D004785:Environmental Pollutants; D004965:Estrogen Antagonists; D004958:Estradiol; D011078:Polychlorinated Biphenyls