Long-term haemopoietic injury in mouse by 1 Gy X-rays at different postconceptional stages.
Haemopoietic effects in mice produced by a single acute dose of 1 Gy X-rays given on the 4th (4R) and 13th (13R) day postconception were evaluated throughout the 9-month post-irradiation. The quality of the stroma was measured in long-term bone marrow cultures (LTBMC). LTBMC granulocyte production was always markedly lower among the irradiated offspring than among controls. IL-6 supernatant levels were significantly higher in both experimental models with respect to controls. However, supernatants from 13R mice had a greater colony-stimulating activity than 4R mice and controls. Granulocytes, from culture or peripheral blood, did not show changes in their functional activity after either treatment. With regard to the content of the femoral granulocyte-macrophage colony forming cells (GM-CFC), there was an enhancement of the GM-colony number as determined from the marrow of 13R mice. In these mice, an alteration in colony size and number due to different combinations of colony-stimulating factors was observed. In summary, our results obtained with irradiation during the foetal period suggest that this 1 Gy X-rays is sufficient to produce measurable, effects on developing murine haemopoiesis.
Escribano
S
S
Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Gaitán
S
S
Cuenllas
E
E
Tejero
C
C
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D005865:Gestational Age; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006410:Hematopoiesis / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009249:NADP / Q000378:metabolism; D011247:Pregnancy; D016897:Respiratory Burst; D013481:Superoxides / Q000378:metabolism; D014965:X-Rays
D016207:Cytokines; D013481:Superoxides; D009249:NADP; D016178:Granulocyte-Macrophage Colony-Stimulating Factor