Cell-to-cell contact activates the long terminal repeat of human immunodeficiency virus 1 through its kappaB motif.
Cell-to-cell contact between peripheral blood lymphocytes and transfected human colonic carcinoma cell line HT29 activates transcription of the long terminal repeats (LTR) of human immunodeficiency virus. HIV-1 LTR transcription is controlled by a complex array of virus-encoded and cellular proteins. Using various constructs expressing a lacZ reporter gene under the control of the intact or three deleted forms of HIV-1 LTR, we obtained evidence that the kappaB regulatory elements located in the U3 region are involved in cell-to-cell activation of HIV-1 LTR. Cell-to-cell contact activates in vitro binding of the nuclear factor kappaB (NF-kappaB) p50/p65 heterodimer to an HIV-1 kappaB oligonucleotide. Cell-to-cell contact activation of NF-kappaB was only partially inhibited by 100 microM pyrrolidine dithiocarbamate and was not correlated with a significant decrease of cellular inhibitor kappaB alpha. NF-kappaB nuclear activation was not detectable before 1 h after cell contact and was dependent on protein synthesis.
Faure
E
E
Institut de Chimie Biologique, Université de Provence, Marseille, France.
Lecine
P
P
Lipcey
C
C
Champion
S
S
Imbert
J
J
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002448:Cell Adhesion; D002450:Cell Communication; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D016322:HIV Enhancer; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007763:Lac Operon; D008214:Lymphocytes; D016328:NF-kappa B / Q000235:genetics; D014162:Transfection
D004279:DNA, Viral; D016328:NF-kappa B