Post-partum flare in MRL-lpr/lpr mice is associated with a parallel increase of N-acetylglucosamine on serum IgG.
Our objective was to investigate IgG glycosylation and disease activity post partum in the MRL-lpr/lpr mouse. Disease activity was monitored using bimalleolar ankle swelling. Levels of galactose and N-acetylglucosamine (GlcNAc) on IgG isolated from serum were measured using biotinylated lectins. Our results show that disease severity increased post partum. This post-partum flare correlated significantly with an increase in IgG GlcNAc expression. The disease severity increased post partum in the MRL-lpr/lpr mouse model similar to the changes seen in rheumatoid arthritis (RA).
Bond
A
A
Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London.
Ratkay
L G
LG
Waterfield
J D
JD
Hay
F C
FC
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D000843:Ankle Joint / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005690:Galactose / Q000378:metabolism; D006031:Glycosylation; D006614:Hindlimb / Q000473:pathology; D007074:Immunoglobulin G / Q000378:metabolism*; D008297:Male; D051379:Mice; D019463:Mice, Inbred MRL lpr; D049590:Postpartum Period; D011247:Pregnancy
D007074:Immunoglobulin G; D000117:Acetylglucosamine; D005690:Galactose