Identification of gene encoding a putative RNA-helicase, homologous to SKI2, in chromosome VII of Saccharomyces cerevisiae.
We have determined the nucleotide sequence of a segment of VII of the yeast Saccharomyces cerevisiae contained in the cosmid clone pEGH101 for a total of 7 kbp. This sequence contains a large open reading frame (ORF) called G9365, coding for a protein of 1967 amino acids that shows a significant homology with the product of the SKI2 gene of S. cerevisiae and contains domains characteristics of RNA-helicases. The ORF is transcribed in vegetative cells but it is not essential for viability as demonstrated by gene disruption.
Martegani
E
E
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Vanoni
M
M
Mauri
I
I
Rudoni
S
S
Saliola
M
M
Alberghina
L
L
D000595:Amino Acid Sequence; D015825:Chromosomes, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000032:analysis; D012331:RNA, Fungal / Q000032:analysis; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal; D014158:Transcription, Genetic
D005656:Fungal Proteins; D012330:RNA, Double-Stranded; D012331:RNA, Fungal; C081929:SKI2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012316:RNA Nucleotidyltransferases; D020365:RNA Helicases