title: Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR.
abstract: A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of 141 isolates, including the 5 group O isolates that were not detected with either of the AMPLICOR HIV-1 test formats.
authors.affiliation: Roche Molecular Systems, Inc., Alameda, California 94501, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Respess
authors.forename: A
authors.initials: A
authors.lastname: Butcher
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Chaowanachan
authors.forename: N
authors.initials: N
authors.lastname: Young
authors.forename: N
authors.initials: N
authors.lastname: Shaffer
authors.forename: T D
authors.initials: TD
authors.lastname: Mastro
authors.forename: B
authors.initials: B
authors.lastname: Biryahwaho
authors.forename: R
authors.initials: R
authors.lastname: Downing
authors.forename: A
authors.initials: A
authors.lastname: Tanuri
authors.forename: M
authors.initials: M
authors.lastname: Schechter
authors.forename: R
authors.initials: R
authors.lastname: Pascu
authors.forename: L
authors.initials: L
authors.lastname: Zekeng
authors.forename: L
authors.initials: L
authors.lastname: Kaptué
authors.forename: L
authors.initials: L
authors.lastname: Gürtler
authors.forename: J
authors.initials: J
authors.lastname: Eberle
authors.forename: D
authors.initials: D
authors.lastname: Ellenberger
authors.forename: C
authors.initials: C
authors.lastname: Fridlund
authors.forename: M
authors.initials: M
authors.lastname: Rayfield
authors.forename: S
authors.initials: S
authors.lastname: Kwok
mesh_terms: D000328:Adult; D014644:Genetic Variation; D016679:Genome, Viral; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*