Isolation and amino acid sequence of flavostatin, a novel disintegrin from the venom of Trimeresurus flavoviridis.
Flavostatin, a novel disintegrin purified from the venom of Trimeresurus flavoviridis, consists of 68 amino acids, including an Arg-Gly-Asp sequence and 12 Cys residues at positions highly conserved among disintegrins. The N-terminal sequence of flavostatin was identical to those of triflavin and flavoridin, previously reported disintegrins from the Trimeresurus flavoviridis venom. Differences among the C-terminal sequences of these disintegrins are considered to affect their biological potencies. Isolated flavostatin inhibited ADP collage, and thrombin receptor agonist peptide-induced platelet aggregation in human platelet-rich plasma with an IC50 range of 59 to 98 nM. Contrary to expectations, these values were similar to those for triflavin.
Maruyama
K
K
Institute for Drug Discovery, Research, Yamanouchi Pharmaceutical Co., Ltd., Ibaraki, Japan.
Kawasaki
T
T
Sakai
Y
Y
Taniuchi
Y
Y
Shimizu
M
M
Kawashima
H
H
Takenaka
T
T
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000494:pharmacology; D003435:Crotalid Venoms / Q000737:chemistry*; D019483:Disintegrins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism; D018179:Receptors, Thrombin / Q000819:agonists / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D003435:Crotalid Venoms; D019483:Disintegrins; D036341:Intercellular Signaling Peptides and Proteins; D009842:Oligopeptides; D010446:Peptide Fragments; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D018179:Receptors, Thrombin; C029748:Trimeresurus venoms; C064594:elegantin; C102800:flavostatin; C070025:triflavin; C069044:flavoridin protein, Trimeresurus; C072032:thrombin receptor peptide (42-55); C089139:trimucrin; D000244:Adenosine Diphosphate; D005340:Fibrinogen; D003094:Collagen; C046617:arginyl-glycyl-aspartyl-serine