Antigen binding specificity of antibodies against 8-methoxypsoralen photoadducted DNA.
The photoadduct between native DNA and 8-methoxypsoralen was characterized on the basis of UV and fluorescence characteristics, Tm, nuclease S 1 digestibility and hydroxyapatite column chromatography. Approximately 80% of photoadduct has at least one diadduct (crosslink) per DNA molecule and only thymine was modified to the extent of 69%. The photo-crosslink was highly immunogenic inducing antibodies having apparent association constant of 1.3 x 10(-9)M. The immune IgG was highly specific towards nucleic acid-furocoumarin crosslink site without appreciable recognition of B-conformation or nucleic acid bases. The antibodies could be used as probe to detect and quantitate nucleic acid modified with furocoumarins in health and disease.
Arif
Z
Z
Department of Biochemistry, Faculty of Medicine, J. N. Medical College, A. M. U. Aligarh, India.
Ali
R
R
D000974:Antibodies, Antinuclear / Q000276:immunology*; D000918:Antibody Specificity; D004247:DNA / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D008730:Methoxsalen / Q000276:immunology / Q000494:pharmacology*; D010775:Photic Stimulation; D017319:Photosensitizing Agents / Q000276:immunology / Q000494:pharmacology*
D000974:Antibodies, Antinuclear; D017319:Photosensitizing Agents; D004247:DNA; D008730:Methoxsalen