Sites of origin and patterns of migration of vasotocin/mesotocin neurons in developing brain of the chick.
Vasotocin/mesotocin (VT/MT) producing neurons are known to migrate extensively during development of the hypothalamus. Birthdating studies as well as immunohistochemical studies suggested the possibility that VT/MT producing neurons originate from specific sites of the neural tube. Furthermore, a relationship between the site of origin and the eventual fate of VT/MT cells has been suggested. This study proposes to identify the sites of origin of VT/MT cells and to establish whether magnocellular and parvocellular VT/MT, and neuromodulatory and neurosecretory VT/MT arise from common or different areas of the developing neural tube. To do so, the embryological distribution of VT/MT producing neurons of the chick was studied with immunohistochemistry. Analysis of the youngest brains in which VT/MT cells could be detected (embryonic day 7.25, E7.25) suggested the presence of two separate sites of origin. The first site was located in the hypothalamic anlage, next to the third ventricle, and the second in the mesencephalon, next to the fourth ventricle. Three-dimensional reconstructions of the location of VT/MT cells throughout development substantiated the hypothesis that diencephalic VT/MT cells originate from the first site while mesencephalic ones originate from the second site. Mesencephalic VT/MT producing cells were confined to the nucleus of Edinger-Westphal and were only detectable during a brief period in development (E7.25-E10). Diencephalic VT/MT producing neurons were noted to form two main paths from their sites of origin to the rostral diencephalon. Quantitative analysis confirmed this caudal to rostral displacement. Magnocellular and parvocellular VT/MT+ cells were intermingled at the diencephalic site of origin as well as in the migratory paths. Neuromodulatory and neurosecretory VT/MT cells of the diencephalon appeared to be derived from a common diencephalic site of origin. These studies support the hypothesis that while specific groups of progenitors may be important in allowing their offspring to produce VT/MT, they do not appear to influence the morphological attributes (magnocellular vs. parvocellular), nuclear locations, or functional characteristics of these cells.
Arnold-Aldea
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SA
Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA. saarnold@bics.bwh.harvard.edu
Sterritt
C
C
D000818:Animals; D000918:Antibody Specificity; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size; D002642:Chick Embryo; D004027:Diencephalon / Q000737:chemistry / Q000166:cytology; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000737:chemistry / Q000166:cytology; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis; D014668:Vasotocin / Q000032:analysis / Q000096:biosynthesis*; D014746:Vimentin / Q000032:analysis
D009419:Nerve Tissue Proteins; D014746:Vimentin; C001713:mesotocin; D010121:Oxytocin; D014668:Vasotocin