title: [1H and 31P-magnetic resonance spectroscopy of cerebral infarction in rats].
abstract: Magnetic resonance spectroscopy (MRS) allows the noninvasive study of metabolism in vivo. In order to further understand the time course of biochemical changes during cerebral infarction, we performed the MRS study with pathological analysis. The left middle cerebral artery (MCA) was occluded in spontaneously hypertensive male rats (SHR) by the method of Tamura et al. The spectra were obtained from the infarcted hemisphere by placing the surface coils over the left side of the calvarium. 31P and 1H-MRS were performed at 3 hours, 24 hours and 7 days after MCA occlusion. Ischemic lesions caused by the left MCA occlusion extended into the parietal lobe and caudate putamen. After 3 hours of ischemia, vacuolated neurophils and shrunken neurons were observed. At 24 hours, these changes were severe. After 7 days, infiltration of monocytes and capillary hyperplasia were seen, and neurons had disappeared. At the acute stage of ischemia the phosphocreatine/inorganic phosphate (PCr/Pi) peak ratio decreased. After 7 days of ischemia, these changes became obscure. The intracellular pH (pHi) decreased after 3 hours of ischemia and recovered almost to the control level at 24 hours post ischemia. Alkalosis was apparent 7 days after ischemia. This alkalosis might be due to increased permeability of the deteriorated blood brain barrier. Although the lactate level was high 24 hours post ischemia, the pHi was almost normal. The N-acetylaspartate/creatine ratio decreased significantly from the acute stage of stroke. This decrease correlated with pathological changes. The correlation of the magnetic resonance spectra with the histological results may opens aspects for monitoring stroke therapy and a new approach to tissue characterization.
authors.affiliation: Second Department of Internal Medicine, First Hospital of Nippon Medical School, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamamuro
authors.forename: Y
authors.initials: Y
authors.lastname: Katayama
authors.forename: H
authors.initials: H
authors.lastname: Igarashi
authors.forename: A
authors.initials: A
authors.lastname: Terashi
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry; D002544:Cerebral Infarction / Q000378:metabolism* / Q000473:pathology; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D010710:Phosphates / Q000032:analysis; D010761:Phosphorus Radioisotopes; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D010710:Phosphates; D010761:Phosphorus Radioisotopes; D006859:Hydrogen