Cytokine production by human soft tissue sarcomas: implications for immunosuppression within the tumour bed.
The local immune response to the presence of tumour is affected by the pattern of cytokine production within the tumour bed. The purpose of this study was to define the pattern of cytokine production in human soft tissue sarcomas, attempting to identify potential immune suppression. Total RNA was extracted from six human soft tissue sarcomas from which cDNA was generated. PCR was carried out in the presence of primer pairs for G3PDH, TGF-beta1, TGF-beta2, TNF-alpha, INF-gamma, IL-2 and IL-10. TGF-beta1 and IL-10 mRNA was detected in all tumours, however, mRNA for IL-2 and INF-gamma was detected in only two out of six sarcomas. Paraffin sections were incubated with alpha-hu-TGF-beta1 or alpha-hu-IL-10 antibodies to localize protein production. TGF-beta1 and IL-10 protein expression was only associated with the tumour cells. These findings suggest potential local immune suppression within the tumour bed.
Spellman
J E
JE
Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Gollnick
S O
SO
Zhang
P J
PJ
Tomasi
T B
TB
D046508:Culture Techniques; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007108:Immune Tolerance; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D016753:Interleukin-10 / Q000032:analysis / Q000096:biosynthesis; D007376:Interleukin-2 / Q000032:analysis / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D012016:Reference Values; D012509:Sarcoma / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis
D016207:Cytokines; D007376:Interleukin-2; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma