VEGF and bFGF mRNA are expressed in ethylnitrosourea-induced experimental rat gliomas.
1. Which angiogenic growth factors actually mediate tumor growth in ethylnitrosourea (ENU)-induced gliomas in rats was examined. 2. In situ hybridization histochemistry with digoxigenin-labeled oligonucleotide probes was used to investigate the cellular expression and distribution of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) mRNAs in ENU-induced gliomas. 3. Both VEGF and bFGF mRNAs were not detected in normal gial cells but in ENU-induced glioma cells. 4. Our results suggest that the growth of ENU-induced glioma may be regulated by multiple angiogenic growth factors and that these gliomas may proliferate by synthesizing such growth factors.
Okimoto
T
T
Department of Pathology, Nagasaki University School of Medicine, Japan.
Shimokawa
I
I
Higami
Y
Y
Ikeda
T
T
D000818:Animals; D001483:Base Sequence; D004076:Digoxigenin / Q000031:analogs & derivatives; D016228:Endothelial Growth Factors / Q000235:genetics*; D005038:Ethylnitrosourea; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D005910:Glioma / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D017403:In Situ Hybridization; D008222:Lymphokines / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental; D009457:Neuroglia; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2; D004076:Digoxigenin; D005038:Ethylnitrosourea