title: Effects of androgen receptor polyglutamine tract expansion on proliferation of NG108-15 cells.
abstract: Expansion of the polyglutamine tracts in the androgen receptor (AR) has been recognized as a cause of X-linked spinal and bulbar muscular atrophy (SBMA). In the present study, NG108-15 cells were stably transfected with expression vectors coding for either the wild type (WT) AR gene (CAG repeat number = 22) or a mutated (MT) AR gene (CAG repeat number = 52). Cells proliferation and cell cycle parameters were evaluated for NG108-15-WT and NG108-15-MT cells in the presence or absence of androgen. NG108-15-WT cells demonstrated an androgen-dependent increase in cell number, while NG108-15-MT cells did not. Our results demonstrate that expansion of polyglutamine tracts in the AR may affect the proliferation and differentiation of nerve cells.
authors.affiliation: First Department of Internal Medicine, Osaka Medical College, Takatsuki City, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
authors.forename: F
authors.initials: F
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: T
authors.initials: T
authors.lastname: Ikemoto
authors.forename: D
authors.initials: D
authors.lastname: Furutama
authors.forename: K
authors.initials: K
authors.lastname: Shinoda
authors.forename: S
authors.initials: S
authors.lastname: Kato
authors.forename: A
authors.initials: A
authors.lastname: Shimizu
authors.forename: N
authors.initials: N
authors.lastname: Ohsawa
mesh_terms: D000728:Androgens / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology; D005910:Glioma; D006822:Hybrid Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D051379:Mice; D016296:Mutagenesis / Q000502:physiology; D009447:Neuroblastoma; D010455:Peptides / Q000378:metabolism*; D010957:Plasmids; D011944:Receptors, Androgen / Q000032:analysis / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000187:drug effects
chemical_list: D000728:Androgens; D010455:Peptides; D011944:Receptors, Androgen; C097188:polyglutamine