title: Purification and characterization of a Na+, K+ ATPase inhibitor found in an endotoxin of Leptospira interrogans.
abstract: We showed previously that the glycolipoprotein fraction prepared from Leptospira interrogans inhibited the Na+,K+ ATPase enzyme purified from brain or kidney and in isolated nephron segments (M. Younes-Ibrahim, P. Burth, M. V. Castro Faria, B. Buffin-Meyer, S. Marsy, C. Barlet-Bas, L. Cheval, and A. Doucet, C. R. Acad. Sci. Paris Ser. III 318:619-625, 1995). In the present communication, we have demonstrated that unsaturated fatty acids such as oleic and palmitoleic acids, which are adsorbed to this fraction, are effective inhibitors of the enzyme.
authors.affiliation: Departamento de Biologia Celular e Genética, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Brazil.
authors.forename: P
authors.initials: P
authors.lastname: Burth
authors.forename: M
authors.initials: M
authors.lastname: Younes-Ibrahim
authors.forename: F H
authors.initials: FH
authors.lastname: Gonçalez
authors.forename: E R
authors.initials: ER
authors.lastname: Costa
authors.forename: M V
authors.initials: MV
authors.lastname: Faria
mesh_terms: D004731:Endotoxins / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D007921:Leptospira interrogans / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
chemical_list: D004731:Endotoxins; D004791:Enzyme Inhibitors; D000254:Sodium-Potassium-Exchanging ATPase