title: Enalapril and captopril enhance antioxidant defenses in mouse tissues.
abstract: This study was conducted to investigate a possible systemic effect of angiotensin-converting enzyme inhibitors (ACEi) on tissue antioxidant defenses. CF1 mice (4-mo-old females) were administered either water (control) or water containing enalapril (20 mg/l) or captopril (50 mg/l) during 11 wk. Neither enalapril nor captopril treatment had an effect on body mass or brain, kidney, or heart weight relative to controls. CuZn-superoxide dismutase (SOD) activity was increased by enalapril treatment in kidney medulla (27%), heart (24%), and erythrocytes (19%) and by captopril treatment in kidney medulla (43%) and heart (54%) relative to controls. Mn-SOD and catalase activities were unaffected by either treatment. Enalapril, but not captopril treatment, increased Se-glutathione peroxidase activity in renal medulla (19%). Nonenzymatic antioxidant defenses, evaluated by tert-butyl hydroperoxide-initiated chemiluminescence (HICL), were enhanced in kidney cortex (48%) by enalapril and in brain by enalapril (44%) or captopril (36%) treatment relative to controls. As evaluated in vitro by HICL and thiobarbituric acid-reactive substances formation, captopril had a free radical scavenger activity, whereas neither enalapril nor lisinopril was effective. These results suggest that ACEi may protect tissues from oxidative damage by increasing enzymatic and nonenzymatic antioxidant defenses.
authors.affiliation: Department of Physical Chemistry, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
authors.forename: E M
authors.initials: EM
authors.lastname: de Cavanagh
authors.forename: C G
authors.initials: CG
authors.lastname: Fraga
authors.forename: L
authors.initials: L
authors.lastname: Ferder
authors.forename: F
authors.initials: F
authors.lastname: Inserra
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002216:Captopril / Q000494:pharmacology*; D004656:Enalapril / Q000494:pharmacology*; D005260:Female; D007668:Kidney / Q000378:metabolism; D017706:Lisinopril / Q000494:pharmacology; D008163:Luminescent Measurements; D051379:Mice; D008815:Mice, Inbred Strains; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010545:Peroxides / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014018:Tissue Distribution; D020122:tert-Butylhydroperoxide
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D010545:Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D004656:Enalapril; D020122:tert-Butylhydroperoxide; D002216:Captopril; D017706:Lisinopril; D010088:Oxidoreductases