Tertiary structural models for human interleukin-6 and evaluation by a sequence-structure compatibility method and NMR experimental information.
Tertiary structure models of Interleukin-6 were constructed using a routine prediction method based on the X-ray crystal structures of granulocyte colony-stimulating factor (GCSF) and leukemia inhibitory factor (LIF). The models were evaluated with the aid of the sequence-structure compatibility (3D-1D) method program compass and NMR experimental information. The model constructed from GCSF gained higher scores on compass examination than did that from LIF, and the NOE data [Nishimura et al. (1996) Biochemistry 35, 273-281] also turned to be more consistent with the former model.
Sumikawa
H
H
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Fukuhara
K
K
Suzuki
E
E
Matsuo
Y
Y
Nishikawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray; D016208:Databases, Factual; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry*; D006131:Growth Inhibitors / Q000737:chemistry; D006801:Humans; D015850:Interleukin-6 / Q000737:chemistry*; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008961:Models, Structural; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D015203:Reproducibility of Results; D017386:Sequence Homology, Amino Acid; D012984:Software
D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D016179:Granulocyte Colony-Stimulating Factor