Molecular cloning and characterization of a new receptor for galanin.
Galanin (GAL) is a widely distributed neuropeptide with diverse biological effects including modulation of hormone release, antinociception and modification of feeding behavior. Its effects are mediated through G-protein-coupled receptors (GPCR) for which only a single type has been cloned, GAL receptor 1 (GALR1). We describe the cloning of a second galanin receptor type, GALR2, from rat hypothalamus. The GALR2 amino acid sequence is 38% identical to GALR1 and is pharmacologically similar to GALR1 when expressed in COS-7 cells. GALR2 is encoded by a single gene containing at least one intron and expressed in a diverse range of tissues.
Howard
A D
AD
Department of Biochemistry and Physiology, Merck Research Laboratories, Rahway, NJ 07065, USA. howarda@merck.com
Tan
C
C
Shiao
L L
LL
Palyha
O C
OC
McKee
K K
KK
Weinberg
D H
DH
Feighner
S D
SD
Cascieri
M A
MA
Smith
R G
RG
Van Der Ploeg
L H
LH
Sullivan
K A
KA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D005810:Multigene Family; D051381:Rats; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone / Q000187:drug effects / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone