[Effect of macrophage colony-stimulating factor on complement receptors and complement regulatory proteins on human peripheral white blood cells].
The effect of M-CSF and C5a on the expression of complement-related membrane proteins on the peripheral white blood cells was investigated. M-CSF or C5a was added into the suspension of the peripheral white blood cells. The expression of the complement receptors, CD35 (CR1) and CD11b/18 (CR3), and inhibitory membrane proteins, DAF and MCP, was measured by flow cytometry. M-CSF increased CR3 on polymorphonuclear cells (PMNs) and CR1, CR3, MCP and DAF on monocytes. C5a increased CR1, CR3 and DAF on PMNs, but did not affect the expression of those on monocytes. It is concluded that M-CSF possessed the activity of increase expression of both complement regulatory proteins and complement receptors of monocytes and C5a selectively affected the expression of those on PMNs.
Nakahara
R
R
First Department of Internal Medicine, Kyoto Prefectural University of Medicine.
Takemura
S
S
Onodera
H
H
Kasamatsu
Y
Y
Seto
N
N
Ichio
N
N
Doi
T
T
Nakanishi
S
S
Okamoto
M
M
Yanagida
K
K
Ueda
M
M
Deguchi
M
M
Kondo
M
M
D000328:Adult; D015703:Antigens, CD / Q000032:analysis*; D015936:Complement C5a / Q000494:pharmacology; D003169:Complement Inactivator Proteins / Q000032:analysis*; D004804:Eosinophils / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000276:immunology*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D016177:Macrophage-1 Antigen / Q000032:analysis; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000032:analysis*; D009000:Monocytes / Q000276:immunology; D011951:Receptors, Complement / Q000097:blood*
D015703:Antigens, CD; C497857:CD46 protein, human; D003169:Complement Inactivator Proteins; D016177:Macrophage-1 Antigen; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D011951:Receptors, Complement; D015936:Complement C5a; D016173:Macrophage Colony-Stimulating Factor