Mapping protein-DNA interactions with CIS-DDP: chromatin structure of promoter region of D. Melanogaster hsp 70 gene.
DNA-protein crosslinking by cis-dichlorodiammineplatinum (II) (cis-DDP) was applied to study chromatin structure in situ. Histone H1 (H5) is crosslinked to DNA in significant amounts whereas core histones remain practically unattached. "Protein image hybridization" experiments show that the 5'-region of the D metanogaster hsp 70 gene is free of histone H1 in both control nuclei and nuclei isolated from heat-shocked embryos.
Belikov
S V
SV
W.A.Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Karpov
V L
VL
D000818:Animals; D002843:Chromatin / Q000235:genetics*; D003432:Cross-Linking Reagents; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D011485:Protein Binding
D002843:Chromatin; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D004247:DNA