Cloning and characterization of the nucleoredoxin gene that encodes a novel nuclear protein related to thioredoxin.
In a yeast artificial chromosome contig close to the nude locus on mouse chromosome 11, we identified a novel gene, nucleoredoxin, that encodes a protein with similarity to the active site of thioredoxins. Nucleoredoxin is conserved between mammalian species, and two homologous genes were found in Caenorhabditis elegans. The nucleoredoxin transcripts are expressed in all adult tissues examined, but restricted to the nervous system and the limb buds in Day 10.5-11.5 embryos. The nucleoredoxin protein is predominantly localized in the nucleus of cells transfected with the nucleoredoxin expression construct. Since the bacterially expressed protein of nucleoredoxin showed oxidoreductase activity of the insulin disulfide bonds with kinetics similar to that of thioredoxin, it may be a redox regulator of the nuclear proteins, such as transcription factors.
Kurooka
H
H
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
Kato
K
K
Minoguchi
S
S
Takahashi
Y
Y
Ikeda
J
J
Habu
S
S
Osawa
N
N
Buchberg
A M
AM
Moriwaki
K
K
Shisa
H
H
Honjo
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D019143:Evolution, Molecular; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D009687:Nuclear Proteins; D013879:Thioredoxins; D010088:Oxidoreductases; C105231:nucleoredoxin