Insertionally inactivated and inducible recA alleles for use in Neisseria.
Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec- and Rec+ by the removal or addition of IPTG. The effects of regulating recA expression on the processes of DNA transformation, DNA repair and pilin-phase variation are described. These regulatable cassettes can also be used to control the expression of any chromosomal gene.
Seifert
H S
HS
Northwestern University Medical School, Department of Microbiology-Immunology, Chicago, IL 60611, USA. h-seifert@nwu.edu
D000483:Alleles; D004352:Drug Resistance, Microbial / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D016254:Mutagenesis, Insertional; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D011938:Rec A Recombinases / Q000235:genetics*
D011938:Rec A Recombinases