RNA polymerase III interferes with Ty3 integration.
Ty3, a gypsylike retrotransposon of budding yeast, integrates at the transcription initiation site of genes transcribed by RNA polymerase III (pol III). It was previously shown that integration in vitro requires intact promoter elements and the pol III transcription factors TFIIIB and TFIIIC. In order to test the effect of pol III on integration, increasing amounts of a pol III-containing fraction were added to Ty3 in vitro integration reactions. The pol III-containing fraction was inhibitory to integration. These results are consistent with a model where the Ty3 integration complex and pol III recognize similar features of the stable transcription complex and compete with each other for access to the transcription initiation site.
Connolly
C M
CM
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92697-4025, USA.
Sandmeyer
S B
SB
D012320:RNA Polymerase III / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism; D018626:Retroelements / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic; D016662:Virus Integration
D016601:RNA-Binding Proteins; D018626:Retroelements; D012343:RNA, Transfer; D012320:RNA Polymerase III