Fractionation and characterization of boar seminal plasma spermadhesion PSP-II glycoforms reveal the presence of uncommon N-acetylgalactosamine-containing N-linked oligosaccharides.
Lectin mapping, carbohydrate analysis and electrospray mass spectrometry of boar seminal plasma PSP-II glycoforms show that its single N-glycosylation site displays a repertoire of carbohydrate structures consisting of the basic pentasaccharide core Man alpha 1-6[Man alpha 1-3]Man beta1-4GlcNAc beta1-4GlcNAc with a fucosyl residue alpha1-6-linked to the innermost N-acetylglucosamine residue. Other glycoforms display fucosylated hybrid-type or monoantennary complex-type chains, some of which contain alpha2-6-linked sialic acid. N-acetylgalactosamine, possibly in Gal beta1-3GalNAc sequence, is present in most of the PSP-II glycoforms.
Solis
D
D
Instituto de Quimíca-Física Rocasolano CSIC, Madrid, Spain.
Calvete
J J
JJ
Sanz
L
L
Hettel
C
C
Raida
M
M
Diaz-Mauriño
T
T
Töpfer-Petersen
E
E
D000117:Acetylglucosamine / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D012661:Semen / Q000737:chemistry*; D029607:Seminal Vesicle Secretory Proteins; D017421:Sequence Analysis; D013552:Swine
D006023:Glycoproteins; D037102:Lectins; D009005:Monosaccharides; D009844:Oligosaccharides; D029607:Seminal Vesicle Secretory Proteins; C074521:seminal vesicle secretory protein II, porcine; D000117:Acetylglucosamine