title: Binding of the type 3 fimbriae of Klebsiella pneumoniae to human endothelial and urinary bladder cells.
abstract: Binding of the two identified type 3 fimbrial variants of Klebsiella pneumoniae to human endothelial EA-hy926 and bladder T24 cells was assessed. The recombinant Escherichia coli strain LE392(pFK12), expressing plasmid-encoded type 3 fimbriae of K. pneumoniae, adhered to both cell lines, and the fimbriae purified from the strain bound to both cell lines in a dose-dependent manner. Adhesiveness to both cell lines of chromosomally encoded type 3 fimbriae from K. pneumoniae IApc35 was lower. No binding was detected with type 1 fimbriae of K. pneumoniae. Both type 3 fimbrial variants exhibited a significantly lower affinity for the cell lines than did S fimbriae of meningitis-associated E. coli.
authors.affiliation: Department of Biosciences, FIN-00014 University of Helsinki, Finland.
authors.forename: A M
authors.initials: AM
authors.lastname: Tarkkanen
authors.forename: R
authors.initials: R
authors.lastname: Virkola
authors.forename: S
authors.initials: S
authors.lastname: Clegg
authors.forename: T K
authors.initials: TK
authors.lastname: Korhonen
mesh_terms: D002460:Cell Line; D004727:Endothelium / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D001743:Urinary Bladder / Q000382:microbiology*
chemical_list: D011994:Recombinant Proteins