title: Frequent expression of interleukin-9 mRNA and infrequent involvement of interleukin-9 in proliferation of primary adult T-cell leukemia cells and HTLV-I infected T-cell lines.
abstract: To examine the possibility that interleukin-9 (IL-9) may be involved in oncogenesis and the proliferation of adult T-cell leukemia (ATL) cells, we examined the expression of IL-9 mRNA and growth response to IL-9 in five human T-lymphotropic virus type-I (HTLV-I) infected T-cell lines and in primary leukemia cells in peripheral blood from eight patients with ATL (four acute ATL and four chronic). Four out of five cell lines expressed IL-9 mRNA not correlated with Tax expression. Primary ATL cells from all patients also expressed IL-9 mRNA not correlated with the clinical forms. Recombinant IL-9 showed growth enhancing activity in only one out of five cell lines and one out of eight patients' primary leukemic cells. These results suggest the infrequent involvement of IL-9 in the proliferation of ATL cells, both primary tumor cells and HTLV-I infected T-cell lines.
authors.affiliation: First Department of Internal Medicine, Kagoshima University, Sakuragaoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Matsushita
authors.forename: N
authors.initials: N
authors.lastname: Arima
authors.forename: H
authors.initials: H
authors.lastname: Ohtsubo
authors.forename: H
authors.initials: H
authors.lastname: Fujiwara
authors.forename: S
authors.initials: S
authors.lastname: Hidaka
authors.forename: J
authors.initials: J
authors.lastname: Fukumori
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D000328:Adult; D002455:Cell Division / Q000502:physiology*; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification*; D006801:Humans; D016906:Interleukin-9 / Q000235:genetics* / Q000502:physiology*; D015458:Leukemia, T-Cell / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology / Q000821:virology*; D014407:Tumor Cells, Cultured
chemical_list: D016906:Interleukin-9; D012333:RNA, Messenger