title: Age-dependent neutrophil and blood flow responsiveness in acute pulmonary inflammation in rabbits.
abstract: Diminished ability of neonatal neutrophils to orient and move in a chemotactic gradient has been linked to compromised pulmonary host defense. We investigated whether deficiency of neonatal neutrophil function in vitro was evident in acute pulmonary inflammation. Analysis of neutrophils in vitro showed impaired chemotaxis in 4-wk-old compared with adult rabbits. In vivo-directed migration of labeled neutrophils into the alveolar space of adult rabbits in response to C5f instillation was significantly less for neutrophils donated from 4-wk-old rabbits compared with those from adults. In contrast, there were no differences in the alveolar accumulation of 4-wk-old and adult labeled neutrophils in 4-wk-old rabbits in response to C5f instillation, although the response showed a shorter time course than seen in adult rabbits. Adult rabbits diverted 46% of the blood away from the right cranial lung lobe, whereas 4-wk-old rabbits showed no change in blood flow after C5f instillation. Megakaryocytes (a source of blood flow mediators) were 3.2-fold greater in adult compared with 4-wk-old lung. These data suggest that the lack of blood flow diversion from inflamed neonatal lung increases neutrophil migration into alveoli, allowing for preservation of an inflammatory response despite neutrophil deficiencies in chemotaxis.
authors.affiliation: Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Hyde
authors.forename: G P
authors.initials: GP
authors.lastname: Downey
authors.forename: F
authors.initials: F
authors.lastname: Tablin
authors.forename: S
authors.initials: S
authors.lastname: Rosengren
authors.forename: P C
authors.initials: PC
authors.lastname: Giclas
authors.forename: P M
authors.initials: PM
authors.lastname: Henson
authors.forename: G S
authors.initials: GS
authors.lastname: Worthen
mesh_terms: D000208:Acute Disease; D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D015936:Complement C5a / Q000008:administration & dosage; D007249:Inflammation / Q000503:physiopathology; D008168:Lung / Q000503:physiopathology*; D008533:Megakaryocytes / Q000473:pathology; D008833:Microcirculation; D009504:Neutrophils / Q000502:physiology*; D011014:Pneumonia / Q000503:physiopathology*; D011652:Pulmonary Circulation; D011817:Rabbits
chemical_list: D015936:Complement C5a