title: Identification of the tetracycline resistance gene, tet(O), in Streptococcus pneumoniae.
abstract: Five isolates of Streptococcus pneumoniae resistant to tetracycline but lacking tet(M) were studied. The tetracycline resistance gene, tet(O), was detected for the first time in the pneumococcus. The gene was amplified and sequenced and found to share 99% nucleotide sequence identity and 99, 99, and 98% deduced amino acid sequence identity with the tet(O) resistance genes of Streptococcus mutans, Campylobacter coli, and Campylobacter jejuni, respectively.
authors.affiliation: MRC/SAIMR/WITS Pneumococcal Diseases Research Unit, South African Institute for Medical Research, Hillbrow, Johannesburg, South Africa.
authors.forename: C A
authors.initials: CA
authors.lastname: Widdowson
authors.forename: K P
authors.initials: KP
authors.lastname: Klugman
authors.forename: D
authors.initials: D
authors.lastname: Hanslo
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D013296:Streptococcus pneumoniae / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; C083139:Tet O resistance protein, Bacteria