Differentiation of eccrine poroma cells to cytokeratin 1- and 10-expressing cells, the intermediate layer cells of eccrine sweat duct, in the tumor cell nests.
It has been shown that an intermediate cell layer exists between a luminal cell layer and a peripheral cell layer in human eccrine sweat ducts by immunohistochemistry using anti-keratin antibodies 34 beta B4 and DE-K10. These antibodies react to cytokeratin 1 and 10 respectively, and stain the intermediate cells specifically, but not luminal cells nor peripheral cells. Cytokeratin 1 and 10 are considered to appear as a differentiated keratin in the terminal process of epidermal keratinization. We examined 5 cases of eccrine poroma with 34 beta B4 and DE-K10. Various numbers of the poroid cells reacted to these anti-keratin antibodies in 4 cases. Some positive cells were observed around the cuticular cells in two of them. The present study demonstrated that terminal differentiation in terms of keratinization can occur in eccrine poromas, and that the 34 beta B4- and DE-K10-positive cells around the cuticular cells differentiate toward the intermediate cells in cytokeratin expression profile and location.
Ban
M
M
Department of Dermatology, Hashima City Hospital, Japan.
Yoneda
K
K
Kitajima
Y
Y
D018250:Acrospiroma / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D004439:Eccrine Glands / Q000033:anatomy & histology / Q000737:chemistry / Q000378:metabolism*; D004817:Epidermis / Q000033:anatomy & histology / Q000737:chemistry; D006651:Histocytochemistry; D006801:Humans; D007633:Keratins / Q000096:biosynthesis* / Q000276:immunology; D013194:Staining and Labeling; D013544:Sweat Gland Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*
D000911:Antibodies, Monoclonal; D007633:Keratins