title: Effect of immunization with an inactivated gp120-depleted HIV-1 immunogen on beta-chemokine and cytokine production in subjects with HIV-1 infection.
abstract: OBJECTIVE
  To measure beta-chemokine and cytokine production in HIV-1-infected subjects undergoing treatment with HIV-1 immunogen (REMUNE).
  
  
  METHODS
  Open label treatment study.
  
  
  METHODS
  beta-Chemokine and cytokine production in peripheral blood mononuclear cell (PBMC) culture.
  
  
  RESULTS
  Interferon-gamma production (p = 0.04) and lymphocyte proliferation (p = 0.001) to HIV-1 antigen-stimulated PBMCs increased after immunization with the HIV-1 immunogen. A correlation was demonstrated after immunization between HIV-1 antigen-stimulated lymphocyte proliferation and interferon-gamma levels (r = 0.53, p = 0.04). No significant change after immunization was seen for interleukin-4 production. A significant increase in mean levels of HIV-1 antigen-stimulated RANTES (i.e., regulated upon, activation normal T-cell expressed and secreted), was evident 1 month after immunization (p = 0.002) and remained elevated 3 months after immunization. RANTES production was decreased in CD8-depleted PBMC cultures. Mean serum HIV-1 RNA copy numbers and CD4 cell counts remained stable after immunization (p > 0.5). A correlation was demonstrated between HIV-1 antigen-stimulated interferon-gamma and RANTES production (r = 0.54, p = 0.002).
  
  
  CONCLUSIONS
  This report describes an augmentation of beta-chemokines and TH1-type cytokines from PBMCs after immunization with the HIV-1 immunogen.
authors.affiliation: Immune Response Corporation, Carlsbad, California 92008, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Moss
authors.forename: R J
authors.initials: RJ
authors.lastname: Trauger
authors.forename: W K
authors.initials: WK
authors.lastname: Giermakowska
authors.forename: J L
authors.initials: JL
authors.lastname: Turner
authors.forename: M R
authors.initials: MR
authors.lastname: Wallace
authors.forename: F C
authors.initials: FC
authors.lastname: Jensen
authors.forename: S P
authors.initials: SP
authors.lastname: Richieri
authors.forename: F
authors.initials: F
authors.lastname: Ferre
authors.forename: A E
authors.initials: AE
authors.lastname: Daigle
authors.forename: C
authors.initials: C
authors.lastname: Duffy
authors.forename: G
authors.initials: G
authors.lastname: Theofan
authors.forename: D J
authors.initials: DJ
authors.lastname: Carlo
mesh_terms: D018791:CD4 Lymphocyte Count; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000032:analysis; D018925:Chemokines / Q000096:biosynthesis*; D016207:Cytokines / Q000096:biosynthesis*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1; D006801:Humans; D007114:Immunization; D007371:Interferon-gamma / Q000032:analysis; D008213:Lymphocyte Activation; D013997:Time Factors
chemical_list: D018946:Chemokine CCL5; D018925:Chemokines; D016207:Cytokines; D015699:HIV Envelope Protein gp120; D007371:Interferon-gamma