Modulated cytokine production from cervical lymph node cells treated with B[a]P and PCB.
To investigate the effects of Benzo[a]pyrene (B[a]P) and polychlorinated biphenyls (PCB) on in vitro cytokine production, we isolated cervical lymph node cells from ovalbumin (OA)-primed mice and the lymph node cells were cultured with various concentrations of B[a]P or PCB in the presence of antigen presenting cells (APC) and OA. B[a]P significantly increased the levels of interleukin-4 (IL-4) and -6(IL-6) in the culture supernatants. However, the levels of interferon-gamma (IFN-gamma) were decreased by treatment with PCB. These findings indicate that in vitro treatment with B[a]P or PCB modulate cytokine production from lymph node cells.
Fujimaki
H
H
National Institute for Environmental Studies, Ibaraki, Japan.
Shiraishi
F
F
Aoki
Y
Y
Saneyoshi
K
K
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D016207:Cytokines / Q000096:biosynthesis*; D004785:Environmental Pollutants / Q000633:toxicity*; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009333:Neck; D011078:Polychlorinated Biphenyls / Q000633:toxicity*
D016207:Cytokines; D004785:Environmental Pollutants; D015850:Interleukin-6; D015847:Interleukin-4; D001564:Benzo(a)pyrene; D007371:Interferon-gamma; D011078:Polychlorinated Biphenyls