Melatonin and serotonin regulate the release of insulin-like growth factor-I, oxytocin and progesterone by cultured human granulosa cells.
The direct influence of indoleamines on ovarian peptide hormones and growth factor secretion, in contrast to steroidogenesis, is yet to be thoroughly investigated. The aim of our in vitro experiments was to investigate the influence of melatonin and serotonin (5-hydroxy-tryptamine) (0.01-10 micrograms/ml) on the release of insulin-like growth factor-I (IGF-I), oxytocin and progesterone by cultured human granulosa cells. It was observed that both melatonin and serotonin stimulate IGF-I release. Melatonin also stimulated oxytocin output. Serotonin increased oxytocin secretion only at the highest dose (10 micrograms/ml). Both melatonin and serotonin were potent inhibitors of progesterone release. The present results suggest a possible involvement of the indoleamines melatonin and serotonin in the direct regulation of growth factor, nonapeptide and steroid hormone secretion by human ovarian cells.
Schaeffer
H J
HJ
Laboratory of Endocrinology, University of Köln, Germany.
Sirotkin
A V
AV
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008550:Melatonin / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D011863:Radioimmunoassay; D012701:Serotonin / Q000494:pharmacology*
D012701:Serotonin; D011374:Progesterone; D010121:Oxytocin; D007334:Insulin-Like Growth Factor I; D008550:Melatonin