title: Effects of the calciotrophic peptides calcitonin and parathyroid hormone on prostate cancer growth and chemotaxis.
abstract: BACKGROUND
  The most common site of metastases in prostate cancer is the skeleton and occurs in 70-80% of patients with prostate carcinoma. Calciotrophic peptides are important in the growth and development of normal bone matrix.
  
  
  METHODS
  Three human prostate carcinoma cells lines, DU-145, PC-3, and LNCaP, were exposed to varying concentrations of parathyroid hormone (PTH) or calcitonin (CT). Cell proliferation and chemotaxis were assessed.
  
  
  RESULTS
  Proliferation increased in LNCaP cells in a dose-dependent manner following treatment with PTH. Proliferation was not altered in PC-3 cells in response to PTH. Proliferation was decreased in DU-145 and PC-3 cells and increased in LNCaP cells after treatment with CT. Cell chemotaxis was increased in the presence of PTH in DU-145 and PC-3 cells compared to vehicle-treated controls.
  
  
  CONCLUSIONS
  The combined proliferation and chemotaxis data suggest that PTH has a dual role in prostate carcinoma resulting in an increase in the number and migration of selected prostate cancer cells. With CT, chemotaxis was unchanged in the DU-145 and PC-3 cells and significantly elevated in the LNCaP cell line. The calciotrophic hormones, PTH and CT, may play an integral role in the regulation of prostate cell growth and metastases.
authors.affiliation: Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: C K
authors.initials: CK
authors.lastname: Ritchie
authors.forename: K G
authors.initials: KG
authors.lastname: Thomas
authors.forename: L R
authors.initials: LR
authors.lastname: Andrews
authors.forename: D J
authors.initials: DJ
authors.lastname: Tindall
authors.forename: L A
authors.initials: LA
authors.lastname: Fitzpatrick
mesh_terms: D002116:Calcitonin / Q000494:pharmacology*; D002277:Carcinoma / Q000188:drug therapy* / Q000503:physiopathology*; D002455:Cell Division / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000503:physiopathology*; D014407:Tumor Cells, Cultured
chemical_list: D010281:Parathyroid Hormone; D002116:Calcitonin