Castor-oil induced diarrhoea in ponies: a model for acute colitis.
A reproducible, reversible model of colitis induced in ponies by administering castor oil (2.5 ml/kg bodyweight [bwt] per os) was characterised by abdominal pain, fever, watery diarrhoea, dehydration, hypovolaemia, toxaemia, leucopenia, decreased serum Cl, Na and K levels and metabolic acidosis. The signs were most severe between 24 and 48 h post induction, stabilisation was frequently observed after 72 h, although diarrhoea could persist beyond 96 h. Morphological and in vitro transport studies (right ventral colon) were conducted on tissues from animals destroyed at 24, 48 and 72 h. In the caecum and colon, surface epithelial disruption and exfoliation from the basement membrane occurred between 24 and 48 h. Early signs of recovery were evident by replenishment of denuded areas with columnar epithelium at 72 h. The crypt epithelium was unaffected throughout the intestinal tract. In vitro transport studies were consistent with the morphological findings. Decreased Na-Cl absorption and normal Cl secretion indicated an impaired surface epithelium coincident with an undamaged cryptal epithelium. Increased mucosal permeability was demonstrated by high ionic conductance and large unidirectional isotopic fluxes. Tissue conductance improved during in vitro incubation suggesting epithelial repair after removal of castor oil. Changes in the population and proportion of bacteria in the faeces as diarrhoea ensued were confirmed at necropsy with a predominance of E. coli and Enterobacter/Klebsiella sp in the large bowel. The experimental induction of castor oil colitis showed many similarities to intestinal endotoxaemia and the secretory type diarrhoea encountered in naturally occurring acute colitis syndromes in horses. The model could prove applicable in studying the pathophysiological mechanisms precipitating such life-threatening disorders.
Roberts
M C
MC
North Carolina State University, College of Veterinary Medicine, Department of Food Animal and Equine Medicine, Raleigh 27606, USA.
Clarke
L L
LL
Johnson
C M
CM
D000138:Acidosis / Q000503:physiopathology / Q000662:veterinary; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001772:Blood Cell Count / Q000662:veterinary; D002368:Castor Oil / Q000009:adverse effects* / Q000494:pharmacology; D002400:Cathartics / Q000009:adverse effects* / Q000494:pharmacology; D002432:Cecum / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D003002:Clonixin / Q000031:analogs & derivatives / Q000494:pharmacology; D003092:Colitis / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D003106:Colon / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D003967:Diarrhea / Q000139:chemically induced / Q000503:physiopathology / Q000662:veterinary*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004573:Electrolytes / Q000032:analysis / Q000097:blood; D004754:Enterobacter / Q000302:isolation & purification; D004848:Epithelium / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D004926:Escherichia coli / Q000302:isolation & purification; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005334:Fever / Q000503:physiopathology / Q000662:veterinary; D006734:Horse Diseases / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D006736:Horses; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008871:Microvilli / Q000648:ultrastructure; D013577:Syndrome
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002400:Cathartics; D004573:Electrolytes; D002368:Castor Oil; C014558:flunixin meglumine; D003002:Clonixin