Effects of tumor necrosis factor on receptor-mediated endocytosis and barrier functions of bovine brain capillary endothelial cell monolayers.
Tumor necrosis factor alpha (TNF-alpha) plays a crucial role in the pathogenesis of the central nervous system infections. In an in vitro reconstructed blood-brain barrier model, a significant dysregulation of receptor mediated endocytosis of low density lipoproteins (LDL) and transferrin (Tf) is demonstrated at delayed phase of direct TNF-alpha activation. Concomitant with the increase in LDL uptake, we demonstrate a decrease of Tf-receptor mediated endocytosis. The potential role of TNF action in the differential or opposite routing of macromolecules is also characterized by a stimulation of their transcytosis. These findings may provide a new insight into the inflammatory effect of TNF-alpha on brain capillary endothelial cells.
Descamps
L
L
Inserm U325-Serlia, Institut Pasteur, Lille, France.
Cecchelli
R
R
Torpier
G
G
D000818:Animals; D001692:Biological Transport; D001812:Blood-Brain Barrier / Q000187:drug effects*; D002196:Capillaries / Q000166:cytology / Q000187:drug effects; D002199:Capillary Permeability / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D003599:Cytoskeleton / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D007444:Inulin / Q000493:pharmacokinetics; D008077:Lipoproteins, LDL / Q000493:pharmacokinetics; D046911:Macromolecular Substances; D051381:Rats; D011990:Receptors, Transferrin / Q000502:physiology*; D013395:Sucrose / Q000493:pharmacokinetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D008077:Lipoproteins, LDL; D046911:Macromolecular Substances; D011990:Receptors, Transferrin; D014409:Tumor Necrosis Factor-alpha; D013395:Sucrose; D007444:Inulin