p53 is not required for regulation of apoptosis or radioprotection by interleukin-3.
Primary interleukin-3 (IL-3)-dependent mast cell cultures from bone marrow of p53-null mice and littermate controls were established. Both p53-null and wild-type cells entered apoptosis on IL-3 removal, showing that p53 is not required for entry into apoptosis after factor deprivation. After X-irradiation, a lower proportion of the p53-null than wild-type cells underwent G2 arrest, but their radiosensitivity was similar. An IL-3-dependent cell line expressing wild-type p53 was used to show that cells die at a fixed time after X-irradiation rather than from a specific cell cycle point.
Silva
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A
CRC Centre for Cell and Molecular Biology, Chester Beatty Laboratories, London, UK.
Wyllie
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A
Collins
M K
MK
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D001854:Bone Marrow Cells; D002453:Cell Cycle; D016195:G2 Phase; D016158:Genes, p53; D007377:Interleukin-3 / Q000494:pharmacology*; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D018345:Mice, Knockout; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology; D011836:Radiation Tolerance / Q000187:drug effects*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*
D007377:Interleukin-3; D016159:Tumor Suppressor Protein p53