Yeast colony size reflects YAC copy number.
A novel strategy for separation of co-cloned YACs was developed. For this, yeast cells were grown under non-selective conditions to allow the mitotic loss of multiple YACs. Yeast colonies of different size appear on 'drop-out' selection plates with small clones consistently containing a single-copy YAC. Different auxotrophic marker genes can be used to separate co-cloned YACs or reduce their copy number, which is essential for most YAC-modification procedures.
Popov
A V
AV
Department of Development and Genetics, The Babraham Institute, Cambridge CB2 4AT, UK. andrei.popov@bbsrc.ac.uk
Bützler
C
C
Brüggemann
M
M
D015139:Blotting, Southern; D018244:Chromosomes, Artificial, Yeast / Q000648:ultrastructure; D003001:Cloning, Molecular / Q000379:methods*; D003470:Culture Media; D005821:Genetic Techniques; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*
D003470:Culture Media