title: Rush immunotherapy results in allergen-specific alterations in lymphocyte function and interferon-gamma production in CD4+ T cells.
abstract: BACKGROUND
  Allergen immunotherapy results in a number of changes in clinical, inflammatory, and immunologic parameters. However, the basis for the specificity of this form of therapy is unknown, especially in the context of changes in T- and B-lymphocyte function after desensitization to specific allergens.
  
  
  OBJECTIVE
  This study was designed to determine the immunologic consequences of rush immunotherapy.
  
  
  METHODS
  We studied 10 patients who had positive skin test responses to the house dust mite Dermatophagoides pteronyssinus (Dpt) and cat dander extract. Each received rush immunotherapy to mite, but not cat dander, over a 2- to 4-week period until maintenance was achieved. Patients were evaluated before and when maintenance was achieved for skin test and nasal reactivity to mite and cat dander; antibody levels to the allergen were monitored, as were lymphocyte proliferative responses and cytokine production.
  
  
  RESULTS
  Rush immunotherapy to house dust mite resulted in a significant reduction in skin and nasal reactivity to mite allergen, but not to cat allergen, in 10 of 10 patients. This was accompanied by a rise in serum anti-Dpt IgE, whereas anti-cat IgE was not altered (7 of 7 patients). In seven of seven patients there was an increase in anti-Dpt IgG4 levels. T-cell proliferative responses to mite antigen were suppressed, and numbers of CD8+ T cells increased in frequency. There was a marked increase in interferon-gamma production, particularly by CD4+ T cells in 10 of 10 patients. The correlation between the increases in interferon-gamma production and the changes in cutaneous reactivity was highly significant.
  
  
  CONCLUSIONS
  We show that rush immunotherapy is immunologically specific in eliciting changes in T- and B-cell responses to the desensitization antigen. The specificity and potential benefit of immunotherapy may be linked to the increase in interferon-gamma production by allergen-activated CD4+ T lymphocytes.
authors.affiliation: Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206, USA.
authors.forename: G
authors.initials: G
authors.lastname: Lack
authors.forename: H S
authors.initials: HS
authors.lastname: Nelson
authors.forename: D
authors.initials: D
authors.lastname: Amran
authors.forename: A
authors.initials: A
authors.lastname: Oshiba
authors.forename: T
authors.initials: T
authors.lastname: Jung
authors.forename: K L
authors.initials: KL
authors.lastname: Bradley
authors.forename: P C
authors.initials: PC
authors.lastname: Giclas
authors.forename: E W
authors.initials: EW
authors.lastname: Gelfand
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000276:immunology / Q000628:therapy; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002415:Cats; D002648:Child; D003888:Desensitization, Immunologic / Q000379:methods*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis*; D008297:Male; D008925:Mites / Q000276:immunology; D009299:Nasal Provocation Tests / Q000379:methods; D012882:Skin Tests / Q000379:methods
chemical_list: D000485:Allergens; D007136:Immunoglobulins; D007371:Interferon-gamma