Mob-1, a Ras target gene, is overexpressed in colorectal cancer.
Mutations in the ras oncogenes have been linked to many different cancers. In contrast to the extensive body of knowledge related to the genetics of ras activation, relatively little is known of the transcriptional events triggered by ras. In previous work we have used differential display to identify Mob-1, a member of alpha-chemokine family, as one of the immediate transcriptional targets following Ras activation. Here, we provide additional experimental evidence to support this finding by the use of an inducible H-ras expression system, the treatment of Ras farnesyl transferase inhibitor and activation of endogenous Ras by serum growth factors. We further demonstrate that IP-10, the human homolog of Mob-1, is overexpressed in the majority of colorectal cancers.
Zhang
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R
The Vanderbilt Cancer Center, Department of Cell Biology, School of Medicine, Vanderbilt University, Nashville, Tennessee 37232-6838, USA.
Zhang
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H
Zhu
W
W
Pardee
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AB
Coffey
R J
RJ
Liang
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P
D019883:Alkyl and Aryl Transferases; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D015179:Colorectal Neoplasms / Q000235:genetics*; D016207:Cytokines / Q000235:genetics*; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D051231:Farnesyltranstransferase; D015972:Gene Expression Regulation, Neoplastic; D017781:Genes, Immediate-Early; D011905:Genes, ras; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D014166:Transferases / Q000037:antagonists & inhibitors
C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D016207:Cytokines; D004791:Enzyme Inhibitors; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051231:Farnesyltranstransferase