title: The nitrate reductase gene isolated from DNA of cultured spinach cells.
abstract: The gene encoding nitrate reductase was cloned from the DNA isolated from cultured spinach (Spinacia oleracea cv. Hoyo) cells and sequenced. The clone contains 7612 nucleotides of the gene which consists of four exons interrupted by three introns. The transcription start site was determined by primer extension analysis and located 193 bp upstream of the ATG translation initiation codon. The 5'-flanking region contains a TATA box and CAAT box.
authors.affiliation: Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University, Matsudo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tamura
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: G
authors.initials: G
authors.lastname: Takeba
authors.forename: T
authors.initials: T
authors.lastname: Satoi
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D017343:Genes, Plant; D008969:Molecular Sequence Data; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018724:Spinacia oleracea / Q000201:enzymology* / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D009565:Nitrate Reductases; D050901:Nitrate Reductase