Cell cycle-dependent activation of telomerase in naturally synchronized culture of a true slime mold, Physarum polycephalum.
Telomeres of Physarum plasmodia did not shorten with numerous repeats of nuclear division, and an apparent activity of telomerase was detected in this organism. In naturally synchronized culture of Physarum plasmodia, an evident activation of telomerase was observed at the late S-phase, just prior to the completion of in vivo DNA replication, and the low telomerase activity was detected throughout the cell cycle. In the nuclei isolated from different phases of synchronized plasmodia, a higher activity of telomerase was also observed at late S-phase. These results clearly show the existence of a cell cycle-dependent regulatory mechanism of telomerase activity in growing, naturally synchronized cells.
Shimada
Y
Y
Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan.
Nakano
M
M
Kanda
N
N
Murakami-Murofushi
K
K
Kim
J K
JK
Ide
T
T
Murofushi
H
H
D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D004789:Enzyme Activation; D016195:G2 Phase; D016839:Physarum polycephalum / Q000166:cytology* / Q000201:enzymology*; D016196:S Phase; D013347:Subcellular Fractions / Q000201:enzymology; D019098:Telomerase / Q000378:metabolism*
D004247:DNA; D019098:Telomerase