title: Siderophore production by Staphylococcus aureus and identification of iron-regulated proteins.
abstract: Siderophore activity of Staphylococcus aureus was detected in an iron-restricted chemically defined medium. The molecular mass of this siderophore, called aureochelin, was 577 Da. Surface-associated proteins of 120, 88, 57, 35, and 33 kDa were mainly expressed under iron restriction conditions. Results showed a relationship between siderophore production and the existence of the 120- and 88-kDa proteins. Western blotting of surface-associated proteins revealed that these proteins were recognized both by patients sera and polyclonal rabbit serum.
authors.affiliation: Bacteriology Laboratory, A. Calmette Hospital, Lille, France.
authors.forename: R J
authors.initials: RJ
authors.lastname: Courcol
authors.forename: D
authors.initials: D
authors.lastname: Trivier
authors.forename: M C
authors.initials: MC
authors.lastname: Bissinger
authors.forename: G R
authors.initials: GR
authors.lastname: Martin
authors.forename: M R
authors.initials: MR
authors.lastname: Brown
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D015169:Colony Count, Microbial; D003470:Culture Media / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007501:Iron / Q000378:metabolism; D007700:Kinetics; D013058:Mass Spectrometry; D017262:Siderophores / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D017262:Siderophores; D007501:Iron