title: Desensitization of the P2X(2) receptor controlled by alternative splicing.
abstract: P2X receptors are ion channels gated by extracellular ATP. We report here cloning of a P2X(2) receptor splice variant (P2X(2-2)) carrying a 207 bp deletion in the intracellular C-terminus and the analysis of the corresponding genomic structure of the P2X(2) gene. P2X(2-2) is as highly expressed as the original P2X(2) sequence in various tissues. ATP-activated currents mediated by heterologous expressed P2X(2) or P2X(2-2) receptors showed significant differences in desensitization time constants and steady-state currents in the continuous presence of ATP. These results imply functional differences between cells differentially expressing these P2X(2) isoforms.
authors.affiliation: Department of Otolaryngology, University of Tübingen, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Brändle
authors.forename: P
authors.initials: P
authors.lastname: Spielmanns
authors.forename: R
authors.initials: R
authors.lastname: Osteroth
authors.forename: J
authors.initials: J
authors.lastname: Sim
authors.forename: A
authors.initials: A
authors.lastname: Surprenant
authors.forename: G
authors.initials: G
authors.lastname: Buell
authors.forename: J P
authors.initials: JP
authors.lastname: Ruppersberg
authors.forename: P K
authors.initials: PK
authors.lastname: Plinkert
authors.forename: H P
authors.initials: HP
authors.lastname: Zenner
authors.forename: E
authors.initials: E
authors.lastname: Glowatzki
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009865:Oocytes / Q000502:physiology; D009928:Organ Specificity; D009925:Organ of Corti / Q000378:metabolism; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology*; D058476:Receptors, Purinergic P2X2; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017384:Sequence Deletion; D013154:Spleen / Q000378:metabolism; D014162:Transfection; D014982:Xenopus laevis
chemical_list: C546401:P2RX2 protein, human; D018048:Receptors, Purinergic P2; D058476:Receptors, Purinergic P2X2; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate