title: Mitotic activity and nuclear DNA damage of large cells in Hodgkin's disease: comparison with the expression of p53 and bcl-2 proteins and the presence of Epstein-Barr virus.
abstract: The roles of the bcl-2 and p53 proteins in Hodgkin's disease (HD) are poorly understood. We therefore compared their detected presence in Hodgkin and Reed-Sternberg/large atypical (H-RS/LA) cells immunohistochemically with the percentages of these cells double-labeled for CD30 and DNA strand breaks (DNA fragmentation index, DFI); mitotic indices (MI); and the EBV infection status. We found a highly significant inverse correlation between the fractions per case of H-RS/LA cells expressing bcl-2/p53 proteins and the DFI of CD30+ elements. No marked effect of these two oncoproteins on MI was noticed, although these parameters and DFI of CD30+ cells were linearly related. EBV infection of H-RS/LA cells exerted only a limited effect on the parameters tested. The results of this study suggest that overexpressed bcl-2 and, to some extent, p53 proteins in H-RS/LA cells of HD primarily counteract deletion of these cells.
authors.affiliation: Institute of Pathologic Anatomy and Histology, University of Siena, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Leoncini
authors.forename: D
authors.initials: D
authors.lastname: Spina
authors.forename: P
authors.initials: P
authors.lastname: Close
authors.forename: C
authors.initials: C
authors.lastname: Minacci
authors.forename: T
authors.initials: T
authors.lastname: Megha
authors.forename: F
authors.initials: F
authors.lastname: De Luca
authors.forename: P
authors.initials: P
authors.lastname: Tosi
authors.forename: S
authors.initials: S
authors.lastname: Pileri
authors.forename: R
authors.initials: R
authors.lastname: Kraft
authors.forename: J A
authors.initials: JA
authors.lastname: Laissue
authors.forename: H
authors.initials: H
authors.lastname: Cottier
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006566:Herpesviridae Infections / Q000150:complications; D004854:Herpesvirus 4, Human / Q000235:genetics; D006651:Histocytochemistry; D006689:Hodgkin Disease / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D017403:In Situ Hybridization; D017730:Ki-1 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014412:Tumor Virus Infections / Q000150:complications
chemical_list: D004273:DNA, Neoplasm; D004279:DNA, Viral; D017730:Ki-1 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53