Exacerbations of clinical symptoms in human immunodeficiency virus type 1-infected patients with multicentric Castleman's disease are associated with a high increase in Kaposi's sarcoma herpesvirus DNA load in peripheral blood mononuclear cells.
The epidemiologic link between multicentric Castleman's disease (MCD) and Kaposi's sarcoma (KS) and the high frequency of KS herpesvirus (KSHV) detection in both diseases raise the question of a role of this new virus in the pathogenesis of MCD. To explore this hypothesis, the KSHV DNA load was investigated in peripheral blood mononuclear cells of 3 human immunodeficiency virus (HIV)-infected patients with MCD at different points during the clinical course. Clinical parameters, such as fever and the presence of lymphadenopathy, were systematically assessed. Hemogram and C-reactive protein level determinations were performed as standard procedures. KSHV DNA load was investigated by means of semiquantitative polymerase chain reaction assay using peripheral blood mononuclear cells of the patients. A correlation between the variation in clinical and biologic parameters related to MCD and KSHV DNA load was found, suggesting a close relationship between KSHV and MCD in HIV-1-infected patients.
Grandadam
M
M
Laboratoire de Virologie, CNRS EP 57, Hôpital Tarnier, Paris, France.
Dupin
N
N
Calvez
V
V
Gorin
I
I
Blum
L
L
Kernbaum
S
S
Sicard
D
D
Buisson
Y
Y
Agut
H
H
Escande
J P
JP
Huraux
J M
JM
D005871:Castleman Disease / Q000150:complications* / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D006679:HIV Seropositivity / Q000150:complications* / Q000503:physiopathology* / Q000821:virology; D015497:HIV-1; D006566:Herpesviridae Infections / Q000097:blood / Q000150:complications*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral