Alteration of cell membrane by stress waves in vitro.
Experiments on the biological effects of laser-induced stress waves indicate that there is a transient increase in the permeability of the cell membrane. A cell viability assay (propidium iodide exclusion) shows that mouse breast sarcoma cells are viable after a stress wave. The kinetics of this transient membrane permeability are measured using time-resolved fluorescence imaging. The efflux of a membrane-impermeable fluorescent probe (calcein) following the application of a 300-bar stress wave implies that there is an increase in the membrane permeability. This efflux ceases within 80 s after a stress wave, suggesting that the membrane is no longer permeable to the fluorescent probe. Fitting the observed kinetics to a simple diffusion model yields an average initial diffusion constant of 2.2 +/- 1.3 x 10(-7) cm2/s for mouse breast sarcoma cells following the application of a laser-induced stress wave.
Lee
S
S
Wellman Laboratories of Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA.
Anderson
T
T
Zhang
H
H
Flotte
T J
TJ
Doukas
A G
AG
D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability; D002470:Cell Survival; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D015200:Fluoroimmunoassay; D008096:Lithotripsy; D008325:Mammary Neoplasms, Experimental / Q000503:physiopathology*; D051379:Mice; D012513:Sarcoma, Experimental / Q000503:physiopathology*; D014407:Tumor Cells, Cultured
D005452:Fluoresceins; D005456:Fluorescent Dyes; C007740:fluorexon