Identification of a 790-kilobase region of common allelic loss in chromosome 10q25-q26 in human endometrial cancer.
We previously identified two commonly deleted regions on chromosome bands 10q25-q26 in endometrial cancer: an 8-cM region and a 12-cM region. In the present study, we further studied the 8-cM region with 83 endometrial cancer specimens and 14 microsatellite markers and narrowed it down to a 1-cM region between D10S587 and D10S1723. This result was confirmed by two-color fluorescence in situ hybridization analysis. An association between histopathologically lower grade tumor and allelic loss (P = 0.03 by Fisher's exact test) was also observed. We also constructed a yeast artificial chromosome (YAC) contig and found that one YAC clone, which was 790 kb in size, harbored the whole 1-cM region of common allelic loss.
Nagase
S
S
Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan.
Yamakawa
H
H
Sato
S
S
Yajima
A
A
Horii
A
A
D000483:Alleles; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats
D005819:Genetic Markers