title: A rapid and simple method for extracting human immunodeficiency virus type 1 RNA from plasma: enhanced sensitivity.
abstract: We present here a rapid and simple technique for processing human immunodeficiency virus (HIV)-infected plasma by high-speed centrifugation. HIV type 1 virions are pelleted from up to 1 ml of plasma, gently lysed with a nonionic detergent, and directly amplified. The procedure has few manipulations and requires approximately 1.5 h for the processing of 24 samples. Viral recovery ranges from 80 to 90%, with an analytical sensitivity approaching 20 copies/ml.
authors.affiliation: Roche Molecular Systems, Alameda, San Francisco, California, USA.
authors.forename: J
authors.initials: J
authors.lastname: Mulder
authors.forename: R
authors.initials: R
authors.lastname: Resnick
authors.forename: B
authors.initials: B
authors.lastname: Saget
authors.forename: S
authors.initials: S
authors.lastname: Scheibel
authors.forename: S
authors.initials: S
authors.lastname: Herman
authors.forename: H
authors.initials: H
authors.lastname: Payne
authors.forename: R
authors.initials: R
authors.lastname: Harrigan
authors.forename: S
authors.initials: S
authors.lastname: Kwok
mesh_terms: D015658:HIV Infections / Q000097:blood / Q000821:virology*; D015497:HIV-1; D006801:Humans; D012367:RNA, Viral / Q000097:blood / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D012367:RNA, Viral