Mitomycin C dissolved in a reversible thermosetting gel: target tissue concentrations in the rabbit eye.
OBJECTIVE
To determine whether a new, reversible thermosetting gel enhances mitomycin C transfer to target ocular tissues in the rabbit eye.


METHODS
A 0.1 ml solution of mitomycin C containing 0.22 microgram, 2.9 micrograms, or 28 micrograms of the agent dissolved in a reversible thermosetting gel consisting of methylcellulose, citric acid, and polyethylene glycol was injected subconjunctivally in 30 New Zealand albino rabbits. Scleral and conjunctival tissues were excised at 0.5, 1, 2, 4, or 24 hours after the injection and mitomycin C concentrations in these tissues were determined by high performance liquid chromatography. The concentration over time was approximated to a single exponential curve, and initial mitomycin C concentrations, time constants, and half life values were determined. Finally, the areas under the curves (AUCs) between 0.5 and 24 hours were calculated.


RESULTS
The mitomycin C concentrations in the target tissues were dose dependent and decreased rapidly over 24 hours. Both the initial mitomycin C concentrations as well as AUCs in these eyes treated with mitomycin C, dissolved in a reversible thermosetting gel, were higher than those in eyes treated similarly in a previous study in which the gel was not used.


CONCLUSIONS
Applied subconjunctivally in the rabbit eye, mitomycin C dissolved in the reversible thermosetting gel enhanced transfer of the agent to the sclera and the conjunctiva.
Ichien
K
K
Department of Ophthalmology, Gifu University School of Medicine, Japan.
Yamamoto
T
T
Kitazawa
Y
Y
Oguri
A
A
Ando
H
H
Kondo
Y
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D019343:Citric Acid / Q000008:administration & dosage; D003228:Conjunctiva / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D005782:Gels; D008747:Methylcellulose / Q000008:administration & dosage; D016685:Mitomycin / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D010592:Pharmaceutic Aids / Q000008:administration & dosage*; D011092:Polyethylene Glycols / Q000008:administration & dosage; D011817:Rabbits; D012590:Sclera / Q000737:chemistry*
D005782:Gels; D010592:Pharmaceutic Aids; D019343:Citric Acid; D011092:Polyethylene Glycols; D016685:Mitomycin; D008747:Methylcellulose