Up-regulation of the intracellular Ca2+ signaling and mRNA expression of platelet-activating factor receptor by estradiol in human uterine endometrial cells.
Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent chemical mediator in inflammation, plays a role in reproduction. Using primary culture of human uterine endometrial cells, we investigated the effect of sex steroid hormones on the PAF-induced signal and its receptor mRNA expression. After a 24 hr treatment with estradiol, PAF increased the intracellular calcium ion ([Ca2+]i) in the glandular cells, but not in the stromal cells. This response was not observed in the non-treated cells, and was blocked by a PAF antagonist, WEB2086. Two types of mRNA (transcript 1 and transcript 2) occurred for PAF receptor by alternative splicing, which are under control of two distinct promoters. Using RT-PCR analysis, it was shown that both transcripts existed in endometrial cells and that estradiol alone or a combination of estradiol and progesterone induced the accumulation of transcript 2, the promoter of which responded to estrogen in our previous studies. The regulation of PAF receptor by sex steroid hormones in human uterine endometrial cells suggests that PAF is involved in the physiological process of reproduction.
Sato
S
S
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
Kume
K
K
Takan
T
T
Mutoh
H
H
Taketani
Y
Y
Shimizu
T
T
D017398:Alternative Splicing / Q000187:drug effects; D001381:Azepines / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D006801:Humans; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism; D014230:Triazoles / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
D001381:Azepines; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D014230:Triazoles; C039351:platelet activating factor receptor; C052518:WEB 2086; D004958:Estradiol; D002118:Calcium