title: Anti-endothelial cell antibodies to the endothelial hybridoma cell line (EAhy926) in systemic lupus erythematosus patients with antiphospholipid antibodies.
abstract: The endothelial hybridoma (EAhy926) cell line was employed to clarify whether antiphospholipid antibodies (aPA) [lupus anticoagulant (LA), antiprothrombin antibody (aPT) and/or anticardiolipin antibody (aCL)] and anti-endothelial cell antibodies (AECA) are identical, and establish whether beta2-glycoprotein I (beta2-GPI) is needed for reactivity of aPA to endothelial cells. Ig-G AECA was positive in 9/30 SLE patients with aPA (30.0%) and 10/22 SLE patients negative for aPA (45.5%). Ig-M AECA was positive in one SLE patient with aPA and one SLE patient without aPA. AECA-positivity was not significantly different among unfixed, TNF-stimulated and fixed EAhy926. The influence of beta2-GPI on the reactivity of serum to EAhy926 was minimal, and absorption experiments of serum with cardiolipin-liposome/beta2-GPI or phosphatidylserine-liposome/prothrombin gave little evidence of cross-reactivity of aPA and AECA. The results of our study suggest that aPA and AECA may have partially cross-reacted, but were different antibodies. However, further study is needed to clarify the clinico-pathological significance of AECA.
authors.affiliation: Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Gotoh
authors.forename: K
authors.initials: K
authors.lastname: Gohchi
authors.forename: K
authors.initials: K
authors.lastname: Kawasugi
authors.forename: M
authors.initials: M
authors.lastname: Tsukamoto
authors.forename: N
authors.initials: N
authors.lastname: Saitoh
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D017153:Antibodies, Anticardiolipin / Q000378:metabolism; D017152:Antibodies, Antiphospholipid / Q000378:metabolism*; D001323:Autoantibodies / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D016682:Lupus Coagulation Inhibitor / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D011516:Prothrombin / Q000276:immunology*; D053482:beta 2-Glycoprotein I
chemical_list: D000906:Antibodies; D017153:Antibodies, Anticardiolipin; D017152:Antibodies, Antiphospholipid; D001323:Autoantibodies; D006023:Glycoproteins; D016682:Lupus Coagulation Inhibitor; C087589:anti-endothelial cell antibody; D053482:beta 2-Glycoprotein I; D011516:Prothrombin