Granulocyte colony-stimulating factor-induced comobilization of CD4- CD8- T cells and hematopoietic progenitor cells (CD34+) in the blood of normal donors.
The feasibility of transplantation of HLA-matched hematopoietic progenitor cells from the blood of normal donors given granulocyte colony-stimulating factor (G-CSF) has been reported recently. In the current study, the changes in T-cell subsets as well as CD34+ cells were determined in one blood volume leukapheresis products of six normal individuals given G-CSF. Examination of the T-cell subsets in the leukapheresis products showed three different patterns: one in which a discrete population of CD4- CD8- alphabeta T cells was found in addition to the typical CD4+ and CD8+ T cells in the unfractionated as well as in high- and low-density cells; a second in which the discrete population of CD4- CD8- alphabeta T cells was predominant only in the low-density fractions; and a third in which a discrete population of CD4- CD8- T cells was not observed. The median yield of CD4- CD8- T cells was about fourfold to fivefold higher than the calculated number present in one blood volume (5L) from normal individuals. The ratios of CD34+ cells to CD4+ and CD8+ T cells, and of CD4- CD8- T cells to CD4+ and CD8+ T cells, were highest in the low-density fractions. These fractions suppressed the mixed leukocyte, and may ameliorate graft-versus-host disease as compared with unfractionated cells.
Kusnierz-Glaz
C R
CR
Department of Medicine, Stanford University School of Medicine, CA 94305-5111, USA.
Still
B J
BJ
Amano
M
M
Zukor
J D
JD
Negrin
R S
RS
Blume
K G
KG
Strober
S
S
D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D001772:Blood Cell Count / Q000187:drug effects; D001782:Blood Donors; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002499:Centrifugation, Density Gradient; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology* / Q000627:therapeutic use; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D007937:Leukapheresis; D008027:Light; D007959:Lymphocyte Culture Test, Mixed; D012542:Scattering, Radiation; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology
D018952:Antigens, CD34; D015704:CD4 Antigens; D016827:CD8 Antigens; D016179:Granulocyte Colony-Stimulating Factor