title: Putative melatonin receptors in the blind mole rat harderian gland.
abstract: The blind mole rat (Spalax ehrenbergi) displays daily and seasonal rhythms. Melatonin, secreted nocturnally by the pineal gland, is also produced in the harderian gland and affects its morphology in rodents. We report here on the presence of putative melatonin receptors in the blind mole rat harderian gland, located in the microsome-enriched fraction of the cells. Equilibrium 125I-melatonin binding studies indicated high- and low-affinity melatonin binding sites in the female (apparent Kd 10 pM and 2.4 nM, respectively) and low-affinity sites in the male (apparent Kd 2.6 nM) mole rat. The binding sites were not significantly affected by season. Castration increased the density of high-affinity binding sites in males and low-affinity binding in females. 125I-melatonin binding to the gonadectomized mole rat preparation was inhibited by serotonin > 2-iodomelatonin > or = memelatonin > 5-methoxytryptamine. The guanine nucleotide analogs, guanosine 5'-O-[3-thio-triphosphate] and guanosine 5'-O-[2-thio-diphosphate], inhibited specific 125I-melatonin binding, whereas 5'-guanylyl imido-diphosphate was less potent. These results indicate for the first time the presence of GTP-sensitive melatonin binding sites in the blind mole rat harderian gland, and suggest that their expression is under control of sex steroids.
authors.affiliation: Department of Neurobiochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Gilad
authors.forename: U
authors.initials: U
authors.lastname: Shanas
authors.forename: J
authors.initials: J
authors.lastname: Terkel
authors.forename: N
authors.initials: N
authors.lastname: Zisapel
mesh_terms: D000818:Animals; D001665:Binding Sites; D002369:Castration; D005260:Female; D006243:Harderian Gland / Q000378:metabolism*; D007457:Iodine Radioisotopes / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D044094:Receptors, Melatonin; D012377:Rodentia / Q000378:metabolism*; D012621:Seasons; D012727:Sex Characteristics
chemical_list: D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D044094:Receptors, Melatonin; D008550:Melatonin