AMP-deaminases from chicken and rabbit muscle: partial primary sequences of homologous 17-kDa CNBr fragments: autorecognition by rabbit anti-[chicken AMPD].
The apparent size (87.5 kDa) of the major polypeptide in freshly isolated chicken muscle AMP deaminase (AMPD.M) was comparable with that predicted from the sequences of the genes for the major muscle isoforms from human and rat. The size of the subunit of AMP deaminase from chicken muscle is indistinguishable from that of the rabbit enzyme. The peptide profiles of cyanogen bromide digests of AMPD.M from chicken and rabbit share a 17-kDa fragment, representing approximately 20% of the intact subunits of these enzymes. The first 25 residues of these fragments are 88.5% identical; the rabbit and chicken segments are greater than 92% and 84% identical, respectively, to the sequences predicted for residues 310-335 for AMPD.M from human and rat. Polyclonal rabbit antisera directed against AMPD.M from chicken breast recognize the full-length AMPD.M polypeptides on immunoblots of extracts of both avian and rabbit muscle, including an antiserum from the rabbit in which the antibody was prepared. The 17-kDa fragments, derived by incomplete cleavage of highly conserved internal segments of the deaminase subunit, share epitopes involved in the autorecognition of rabbit AMPD.M by rabbit polyclonal antibodies directed against the avian AMPD.M.
Chilson
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OP
Department of Biology, Washington University, St Louis, MO 63130, USA.
Kelly-Chilson
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AE
Siegel
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NR
D000659:AMP Deaminase / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002645:Chickens; D017124:Conserved Sequence; D003488:Cyanogen Bromide / Q000737:chemistry / Q000378:metabolism; D000939:Epitopes; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology*; D010446:Peptide Fragments / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017386:Sequence Homology, Amino Acid
D000906:Antibodies; D000939:Epitopes; D010446:Peptide Fragments; D000659:AMP Deaminase; D003488:Cyanogen Bromide