Human chromosome-specific cDNA libraries: new tools for gene identification and genome annotation.
To date, only a small percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome 5-specific cDNA libraries have been constructed from five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome 5 genes and 138 are from new chromosome 5 genes (a frequency of 79.5%). Sequence complexity estimates indicate that each library contains -20% of the approximately 5000 genes that are believed to reside on chromosome 5. This study more than doubles the number of genes mapped to chromosome 5 and describes an important new tool for disease gene isolation.
Del Mastro
R G
RG
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-8591, USA.
Wang
L
L
Simmons
A D
AD
Gallardo
T D
TD
Clines
G A
GA
Ashley
J A
JA
Hilliard
C J
CJ
Wasmuth
J J
JJ
McPherson
J D
JD
Lovett
M
M
D001483:Base Sequence; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D018076:DNA, Complementary; D015723:Gene Library; D015894:Genome, Human; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012313:RNA
D018076:DNA, Complementary; C034477:RNA primers; D012313:RNA