Flow-injection analysis of cobalt(II) utilizing enhanced lophine chemiluminescence with hydroxylammonium chloride.
A rapid and convenient flow-injection method is described for the determination of Co(II). The method utilized the phenomenon that the lophine-Co(II)-H2O2 chemiluminescence (CL) reaction is enhanced in alkaline media by the addition of hydroxylammonium chloride. The calibration curve was linear over the range 2 x 10(-7) to 2 x 10(-5) M with a detection limit of 4.5 x 10(-8) M (0.9 pmol) at a signal-to-noise ratio of 2. The method was applied to the assay of cyanocobalamin in commercially available eye lotions.
Nakashima
K
K
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Yamasaki
H
H
Shimoda
R
R
Kuroda
N
N
Akiyama
S
S
Baeyens
W R
WR
D002853:Chromatography, Liquid / Q000379:methods; D003035:Cobalt / Q000032:analysis*; D005123:Eye; D017022:Flow Injection Analysis / Q000379:methods*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000737:chemistry; D007093:Imidazoles; D008163:Luminescent Measurements; D009824:Ointments / Q000737:chemistry; D009883:Ophthalmic Solutions / Q000737:chemistry; D014805:Vitamin B 12 / Q000032:analysis
D006898:Hydroxylamines; D007093:Imidazoles; D009824:Ointments; D009883:Ophthalmic Solutions; D019811:Hydroxylamine; D003035:Cobalt; D014805:Vitamin B 12; C107378:lophine