Transcriptional and translational control over sodium-glucose-linked transporter (SGLT1) gene expression in adult rat small intestine.
We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGLT1 mRNA along jejunal villi with previous estimates of SGLT1 protein and SGLT1-mediated glucose transport [Debnam et al., Eur. J. Physiol. 430 (1995) 151-159]. This is the first time it has been possible to directly relate these three aspects of SGLT1 gene expression in a single species. Results are discussed in terms of a possible time rather than positional control over translation of SGLT1 mRNA.
Dong
R
R
Department of Biochemistry and Molecular Biology, Royal Free Hospital School of Medicine, London, UK.
Srai
S K
SK
Debnam
E
E
Smith
M
M
D000818:Animals; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism; D051269:Sodium-Glucose Transporter 1; D014158:Transcription, Genetic
D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D012964:Sodium; D005947:Glucose