Gene expressions of basic fibroblast growth factor and its receptor in healing of rat retina after laser photocoagulation.
Basic fibroblast growth factor (bFGF) stimulates the mitogenesis of various cells and plays a key role in wound repair. Using in situ hybridization, we studied mRNA expressions of bFGF and one of its receptors, FGF receptor 1 (FGFR1), during wound repair of the rat retina after laser photocoagulation. Gene expressions of bFGF and FGFR1 were detected in the ganglion cell and inner nuclear layers of the normal adult rat retina. On day 3 following laser photocoagulation, proliferating retinal pigment epithelial (RPE) cells of the lesion showed intense gene expressions of bFGF and FGFR1. Macrophage-like cells that migrated into the lesion also showed gene expression of bFGF. These gene expressions decreased over time. The finding of elevated gene expressions of bFGF and FGFR1 after laser photocoagulation suggests the bFGF may be a factor in retinal wound repair.
Yamamoto
C
C
Department of Ophthalmology, Kansai Medical University, Osaka, Japan.
Ogata
N
N
Matsushima
M
M
Takahashi
K
K
Miyashiro
M
M
Yamada
H
H
Maeda
H
H
Uyama
M
M
Matsuzaki
K
K
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D005451:Fluorescein Angiography; D005500:Follow-Up Studies; D005654:Fundus Oculi; D015870:Gene Expression; D017403:In Situ Hybridization; D017075:Laser Coagulation; D008297:Male; D010857:Pigment Epithelium of Eye / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D011914:Rats, Inbred BN; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D012160:Retina / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D014945:Wound Healing / Q000502:physiology*
D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; C496350:Fgfr1 protein, rat; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1