Nitrotyrosine bound to beta-VLDL-apoproteins: a biomarker of peroxynitrite formation in experimental atherosclerosis.
Lipoprotein oxidation plays a key role in the initiation and progression of atherosclerosis. Peroxynitrite is a powerful oxidant and nitrating species formed by the reaction of nitric oxide with superoxide radical. Peroxynitrite can oxidize lipoproteins and generate nitrotyrosine either from free or protein-bound tyrosine. Nitrotyrosine has been used as a fingerprint for peroxynitrite reaction in vivo. In this study, the content of nitrotyrosine bound to beta-VLDL apoproteins was determined in New Zealand rabbits before and at 15, 30, 45 and 60 days of cholesterol feeding. A significant increase of nitrotyrosine bound to beta-VLDL apoproteins was observed in parallel with the hypercholesterolemia induced by 1% cholesterol enriched diet. These data indicate that apolipoprotein-bound nitrotyrosine may be used as a biomarker of peroxynitrite production during the development of atherosclerosis in this experimental model.
Moriel
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Department of Clinical and Toxicological Analysis, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
Abdalla
D S
DS
D000818:Animals; D001161:Arteriosclerosis / Q000097:blood / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D006937:Hypercholesterolemia / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood / Q000378:metabolism*; D008297:Male; D009566:Nitrates / Q000097:blood / Q000378:metabolism*; D011817:Rabbits; D014280:Triglycerides / Q000097:blood; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism
D015415:Biomarkers; D001798:Blood Proteins; D008079:Lipoproteins, VLDL; D009566:Nitrates; D014280:Triglycerides; C039642:peroxynitric acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D002784:Cholesterol