title: Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF.
abstract: The vertebrate transcription factors TCF (T cell factor) and LEF (lymphocyte enhancer binding factor) interact with beta-catenin and are hypothesized to mediate Wingless/Wnt signaling. We have cloned a maternally expressed Drosophila TCF family member, dTCF. dTCF binds a canonical TCF DNA motif and interacts with the beta-catenin homolog Armadillo. Previous studies have identified two regions in Armadillo required for Wingless signaling. One of these interacts with dTCF, while the other constitutes a transactivation domain. Mutations in dTCF and expression of a dominant-negative dTCF transgene cause a segment polarity phenotype and affect expression of the Wingless target genes engrailed and Ultrabithorax. Epistasis analysis positions dTCF downstream of armadillo. The Armadillo-dTCF complex mediates Wingless signaling as a bipartite transcription factor.
authors.affiliation: Department of Immunology, University Hospital, Utrecht, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: van de Wetering
authors.forename: R
authors.initials: R
authors.lastname: Cavallo
authors.forename: D
authors.initials: D
authors.lastname: Dooijes
authors.forename: M
authors.initials: M
authors.lastname: van Beest
authors.forename: J
authors.initials: J
authors.lastname: van Es
authors.forename: J
authors.initials: J
authors.lastname: Loureiro
authors.forename: A
authors.initials: A
authors.lastname: Ypma
authors.forename: D
authors.initials: D
authors.lastname: Hursh
authors.forename: T
authors.initials: T
authors.lastname: Jones
authors.forename: A
authors.initials: A
authors.lastname: Bejsovec
authors.forename: M
authors.initials: M
authors.lastname: Peifer
authors.forename: M
authors.initials: M
authors.lastname: Mortin
authors.forename: H
authors.initials: H
authors.lastname: Clevers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051186:Armadillo Domain Proteins; D019521:Body Patterning / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D017344:Genes, Insect; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D051821:Lymphoid Enhancer-Binding Factor 1; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*; D051155:Wnt1 Protein
chemical_list: C495305:ARM protein, Drosophila; D051186:Armadillo Domain Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006609:High Mobility Group Proteins; D019476:Insect Proteins; D051821:Lymphoid Enhancer-Binding Factor 1; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051155:Wnt1 Protein; C115159:pan protein, Drosophila; C074622:wg protein, Drosophila