title: Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes.
abstract: The present studies were designed to test the hypothesis that mechanical deformation of polymorphonuclear leukocytes (PMN) leads to functional changes that might influence their transit in the pulmonary capillaries. Human leukocytes were passed through 5- or 3-micron-pore polycarbonate filters under controlled conditions. Morphometric analysis showed that the majority of PMN were deformed and that this deformation persisted longer after filtration through 3-micron filters than through 5-micron filters (P < 0.05) but did not result in the cytoskeletal polarization characteristic of migrating cells. Flow cytometric studies of the filtered PMN showed that there was a transient increase in the cytosolic free Ca2+ concentration after both 3- and 5-micron filtration (P < 0.01) with an increase in F-actin content after 3-micron filtration (P < 0.05). Although L-selectin expression on PMN was not changed by either 5- or 3-micron filtration, CD18 and CD11b were increased by 3-micron filtration (P < 0.05). Priming of the PMN with N-formyl-methionyl-leucyl-phenylalanine (0.5 nM) before filtration resulted in an increase of CD11b by both 5 (P < 0.05)- and 3-micron (P < 0.01) filtration. Neither 5- nor 3-micron filtration induced hydrogen peroxide production. We conclude that mechanical deformation of PMN, similar to what occurs in the pulmonary microvessels, induces both structural and functional changes in the cells, which might influence their passage through the pulmonary capillary bed.
authors.affiliation: University of British Columbia Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: S F
authors.initials: SF
authors.lastname: Van Eeden
authors.forename: D M
authors.initials: DM
authors.lastname: Redenbach
authors.forename: M
authors.initials: M
authors.lastname: Daya
authors.forename: B A
authors.initials: BA
authors.lastname: Walker
authors.forename: M E
authors.initials: ME
authors.lastname: Klut
authors.forename: B R
authors.initials: BR
authors.lastname: Wiggs
authors.forename: J C
authors.initials: JC
authors.lastname: Hogg
mesh_terms: D000199:Actins / Q000502:physiology; D018821:CD18 Antigens / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology; D048429:Cell Size; D005374:Filtration; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D019041:L-Selectin / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D009504:Neutrophils / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D011312:Pressure; D017382:Reactive Oxygen Species / Q000378:metabolism; D013314:Stress, Mechanical
chemical_list: D000199:Actins; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016177:Macrophage-1 Antigen; D017382:Reactive Oxygen Species; D019041:L-Selectin; D006861:Hydrogen Peroxide; D002118:Calcium