O-acetylated oligosaccharides from pectins of potato tuber cell walls.
Acetylated trigalacturonides and rhamnogalacturonan I (RG-I)-derived oligosaccharides were isolated from a Driselase digest of potato tuber cell walls by ion-exchange and size-exclusion chromatography. The oligosaccharides were structurally characterized by fast atom bombardment-mass spectroscopy, nuclear magnetic resonance spectroscopy, and glycosyl-linkage composition analysis. One trigalacturonide contained a single acetyl group at O-3 of the reducing galacturonic acid residue. A second trigalacturonide contained two acetyl substituents, which were located on O-3 or O-4 of the nonreducing galacturonic acid residue and O-3 of the reducing galacturonic acid residue. RG-I backbone-derived oligomers had acetyl groups at O-2 of the galacturonic acid residues. Some of these galacturonic acid residues were O-acetylated at both O-2 and O-3 positions. Rhamnosyl residues of RG-I oligomers were not acetylated.
Ishii
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T
Forestry and Forest Products Research Institute, Ibaraki, Japan. tishii@ffpri.affrc.go.jp
D000107:Acetylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D009844:Oligosaccharides; D010368:Pectins