Ammonium containing buffers should be avoided during enzymatic release of glycans from glycoproteins when followed by reducing terminal derivatization.
Küster
B
B
Harvey
D J
DJ
D000581:Amidohydrolases / Q000378:metabolism; D016477:Artifacts; D002021:Buffers; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002021:Buffers; D006023:Glycoproteins; D009844:Oligosaccharides; D000644:Quaternary Ammonium Compounds; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase