title: Expression of dendritic BC200 RNA, component of a 11.4S ribonucleoprotein particle, is conserved in humans and simians.
abstract: Primate BC200 RNA, a brain-specific small cytoplasmic RNA, is one of the few known cell type specific non-messenger RNAs. It originated from a monomeric Alu short interspersed repetitive element (SINE) in primates. In situ hybridization using rhesus monkey (Macaca mulatta) brain sections reveals a similar cellular and sub-cellular distribution as in human brain. In addition to confirming its dendritic location, the distribution in an old world monkey indicates a discrete regional and subcellular location of BC200 RNA. We also report that BC200 RNA exists as a ribonucleoprotein (RNP) particle in vivo. In sucrose gradients, the BC200 particle has a sedimentation constant of about 11.4 S, significantly more than the corresponding 200 nucleotide long naked RNA (approximately 7.6 S).
authors.affiliation: Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: Cheng
authors.forename: H
authors.initials: H
authors.lastname: Tiedge
authors.forename: J
authors.initials: J
authors.lastname: Brosius
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D008253:Macaca mulatta; D012342:RNA, Small Nuclear / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*
chemical_list: D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins