Increased levels of alpha V-associated integrins in association with growth inhibition of cultured tumor cells by bromodeoxyuridine.
Treatment of three small-round-cell-tumor cell lines, TC-32 (peripheral neuroepithelioma), 6647 (Ewing's sarcoma), and GOTO (neuroblastoma), with bromodeoxyuridine (BrdU) (5 micrograms/ml) for 6 days markedly inhibited cell growth in both culture medium and soft agar and induced morphological alteration into large flat cells. These BrdU-treated cells showed markedly increased levels of alpha V-associated integrins, including alpha V beta 3, and no uniform changes in other beta 1 subfamilies (alpha 1-6). Cell attachment to vitronectin was found to be increased in these BrdU-treated cells. These results suggest that increased levels of alpha V-associated integrins are associated with growth inhibition of cultured tumor cells induced by BrdU.
Yoshihara
T
T
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
Hibi
S
S
Tsunamoto
K
K
Todo
S
S
Imashuku
S
S
D000728:Androgens / Q000494:pharmacology; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D016023:Integrins / Q000032:analysis*; D009369:Neoplasms / Q000737:chemistry* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000728:Androgens; D000964:Antimetabolites, Antineoplastic; D016023:Integrins; D001973:Bromodeoxyuridine