Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components.
Assembly of a sequence-specific RNA-protein complex on the 3' untranslated region (3'UTR) of human alpha-globin mRNA (alpha-complex) correlates with mRNA stabilization. Here we map a limited segment of the alpha-globin 3'UTR that is both necessary and sufficient for alpha-complex formation. The sequence of this binding region identifies three additional, highly stable mRNAs that share closely related, pyrimidine-rich cis-motifs in their respective 3'UTRs. Each mRNA assembles a sequence-specific ribonucleoprotein complex at this conserved region. These complexes are structurally related, and each contains a 39-kDa cytoplasmic poly(C) binding protein previously demonstrated to be essential to formation of the alpha-complex. These observations indicate the existence of a general determinant for stabilization of eukaryotic mRNAs.
Holcik
M
M
Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Liebhaber
S A
SA
D001483:Base Sequence; D003094:Collagen / Q000235:genetics; D016384:Consensus Sequence; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D008084:Lipoxygenase / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D016914:Ribonuclease H; D006163:Ribonuclease T1; D012261:Ribonucleoproteins / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics
D012333:RNA, Messenger; D012261:Ribonucleoproteins; D005914:Globins; D003094:Collagen; D008084:Lipoxygenase; D014446:Tyrosine 3-Monooxygenase; D016914:Ribonuclease H; D006163:Ribonuclease T1