Involvement of polyamines in the protection of taurine against the cytotoxicity of hydrazine or carbon tetrachloride in isolated rat hepatocytes.
Taurine (2-aminoethanesulfonic acid) is known to protect hepatocyte injury induced by hydrazine or carbon tetrachloride. We investigated whether cellular polyamines are involved in the protective mechanism of taurine in the hepatocyte injury caused by hydrazine or carbon tetrachloride. The agents decreased cellular polyamine concentrations, but the treatment with taurine prevented this decrease. The protection of taurine against hepatic injury was not observed in hepatocytes treated with alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase which is a key enzyme in polyamine biosynthesis. The protection of taurine was recovered by the addition of polyamines to DFMO-treated hepatocytes. These results suggest that cellular polyamines play an important role in the protection of taurine in hydrazine or carbon tetrachloride-induced hepatocyte injury.
Wu
C
C
Department of Food and Nutrition, Faculty of Human Life Science, Osaka City University, Japan.
Miyagawa
C
C
Kennedy
D O
DO
Yano
Y
Y
Otani
S
S
Matsui-Yuasa
I
I
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002478:Cells, Cultured; D006834:Hydrazines / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology*
D006834:Hydrazines; D011073:Polyamines; D013654:Taurine; C029424:hydrazine; D013096:Spermine; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase; D013095:Spermidine; D011700:Putrescine