The search for novel anticancer agents: a differentiation-based assay and analysis of a folklore product.
One alternative approach to the current use of cytotoxic anticancer drugs involves the use of differentiation-inducing agents. However, a wider application of this strategy would require the development of assays to search for new differentiation-inducing agents. In this report we describe an in vitro assay using the murine erythroleukemia (clone 3-1) cells. Tests for the efficacy of this assay for the analysis of antineoplastic activity in natural products led to studies on pau d'arco, a South American folklore product used in the treatment of cancer. Purification of the activity in aqueous extracts by solvent partition and thin layer chromatography (TLC) indicated the presence of two activities, one of which was identified as lapachol. The activity in the pau d'arco extracts and of lapachol was inhibited by vitamin K1. As a vitamin K antagonist, lapachol might target such vitamin K-dependent reactions as the activation of a ligand for the Axl receptor tyrosine kinase.
Dinnen
R D
RD
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
Ebisuzaki
K
K
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D051379:Mice; D009285:Naphthoquinones / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014812:Vitamin K / Q000037:antagonists & inhibitors
D000970:Antineoplastic Agents; D009285:Naphthoquinones; D014812:Vitamin K; C008252:lapachol