title: Naive alloreactive CD8 T cells are activated by purified major histocompatibility complex class I and antigenic peptide.
abstract: In this report, we demonstrate stimulation of T cell receptor (TCR) transgenic CD8 T cells by isolated major histocompatibility complex (MHC) class I H-2Ld complexes and antigenic peptide. This is the first demonstration of CD8 T cells activated by MHC and antigenic peptide in the absence of antigen priming. Furthermore, isolated MHC and a potent peptide antigen can stimulate phenotypically naive CD44- T cells to become CTL effectors and to produce interleukin-2 in nanogram per milliliter amounts. These results demonstrate that particular TCR antigen pairs may overcome the need for specialized antigen-presenting cells and have implications for mechanisms of autoimmunity and tolerance induction.
authors.affiliation: Division of Monoclonal Antibodies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA.
authors.forename: J
authors.initials: J
authors.lastname: Goldstein
authors.forename: H
authors.initials: H
authors.lastname: Mostowsky
authors.forename: J
authors.initials: J
authors.lastname: Tung
authors.forename: H
authors.initials: H
authors.lastname: Hon
authors.forename: M
authors.initials: M
authors.lastname: Brunswick
authors.forename: S
authors.initials: S
authors.lastname: Kozlowski
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006183:H-2 Antigens / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D064532:Histocompatibility Antigen H-2D; D018960:Hyaluronan Receptors; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000096:biosynthesis; D007519:Isoantigens / Q000276:immunology; D007655:Ketoglutarate Dehydrogenase Complex / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009842:Oligopeptides / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D017186:Titrimetry
chemical_list: D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D064532:Histocompatibility Antigen H-2D; D018960:Hyaluronan Receptors; D007376:Interleukin-2; D007519:Isoantigens; D009842:Oligopeptides; C088354:p2Ca peptide; D007655:Ketoglutarate Dehydrogenase Complex