[The inhibitory effects of Arg-Gly-Asp-Ser on attachment of fibroblasts].
Trabeculectomy often fails because of scarring of the filtering bleb, which is caused by the proliferation of fibroblasts. In this study, we investigated the inhibitory effect of Arg-Gly-Asp-Ser (RGDS) oligopeptide, a cell binding domain of fibronection molecules, on the attachment of fibroblasts in vitro and on scarring of filtering bleb after trabeculectomy on rabbits' eyes. The following results were obtained. 1. RGDS inhibited the attachment of rabbit conjunctival fibroblasts significantly (p < 0.01) when it was added to the culture medium at final concentrations of 2 and 5 mg/ml. 2. The administration of 0.2 ml of RGDS solution (5 mg/ml) into the filtering bleb 3 days after surgery caused significant reductions of intra ocular pressure compared with the control maintained the bleb more than 13 days whereas the blebs of the control eyes disappeared within 6 days (p < 0.01). 3. No complications were caused on the eyeballs of the rabbits by the administration of RGDS.
Kawamura
T
T
Department of Ophthalmology, Osaka Prefectural Habikino Hospital, Japan.
Nakamura
T
T
Kiritooshi
A
A
Yamamoto
R
R
Sasabe
T
T
Kishida
K
K
Yuasa
T
T
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D003228:Conjunctiva / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects*; D007429:Intraocular Pressure / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D014130:Trabeculectomy
D009842:Oligopeptides; D010975:Platelet Aggregation Inhibitors; C046617:arginyl-glycyl-aspartyl-serine