title: An essential tryptophan residue of green crab (syclla serrata) alkaline phosphatase.
abstract: The tryptophan residues in green crab (scylla serrata) alkaline phosphatase (EC 3.1.3.1) have been modified by N-bromosuccinimide (NBS). The modification of five tryptophan residues leads to complete loss of enzymatic activity. With the increase of NBS concentration, both the absorption at 278 nm and the fluorescence emission intensity at 335 nm of the modified enzyme decreased markedly indicating the modification of tryptophan residues. Quantitative treatment of the data (Tsou, Sci. Sinica 1962, 11, 1535-1558) shows that among the tryptophan residues modified, one is essential for its catalytic activity. The presence of the substrate markedly protects the modification of tryptophan residues as well as the inactivation, suggesting that the essential tryptophan residue is situated at the active site of this enzyme.
authors.affiliation: Department of Biology, Xiamen University, China.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Zheng
authors.forename: Q X
authors.initials: QX
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: H M
authors.initials: HM
authors.lastname: Zhou
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003386:Brachyura / Q000201:enzymology*; D001974:Bromosuccinimide / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D013053:Spectrophotometry; D014364:Tryptophan / Q000032:analysis / Q000378:metabolism*
chemical_list: D014364:Tryptophan; D000469:Alkaline Phosphatase; D001974:Bromosuccinimide