title: Incorporation of antibacterial monomer MDPB into dentin primer.
abstract: The polymerizable monomer methacryloyloxydodecylpyridinium bromide (MDPB) shows antibacterial activity when immobilized in a resin-based material. In this study, the antibacterial effect of a dentin primer incorporating MDPB was investigated. The influence of incorporation of MDPB on bond strength to dentin and on the curing performance of the adhesive system was also evaluated. Experimental primers were prepared by addition of MDPB into a proprietary primer at 1, 2, or 5%. Antibacterial effects of experimental primers were compared with those of control primer and two other proprietary primers by an agar disc-diffusion method and bactericidal activity test. Experimental primers produced greater inhibition zones against Streptococcus mutans, Actinomyces viscosus, and Lactobacillus casei than any of three proprietary primers, and inhibition increased as the concentration of MDPB was increased. Bactericidal activity of MDPB-containing primers against Streptococcus mutans was greater than those of the other three primers, with incorporation of MDPB at 5% showing complete killing of bacteria after 30 s contact. No decrease in tensile bond strength was observed for materials containing MDPB. On the contrary, the primer incorporating 1 and 2% MDPB showed higher bond strength than all the others, including the control (p < 0.05). When the degree of conversion of the complex of primer and adhesive resin was determined with Fourier Transform Infrared Spectroscopy, there were no significant differences between any of the experimental primers and the control (p > 0.05). These results indicate that incorporation of the antibacterial monomer MDPB enhanced the antibacterial effect of a proprietary dentin primer before curing, and had no adverse influence on bond strength to dentin and curing of the adhesive system.
authors.affiliation: Department of Operative Dentistry, Osaka University Faculty of Dentistry, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Imazato
authors.forename: Y
authors.initials: Y
authors.lastname: Kinomoto
authors.forename: H
authors.initials: H
authors.lastname: Tarumi
authors.forename: M
authors.initials: M
authors.lastname: Torii
authors.forename: R R
authors.initials: RR
authors.lastname: Russell
authors.forename: J F
authors.initials: JF
authors.lastname: McCabe
mesh_terms: D016924:Actinomyces viscosus / Q000187:drug effects; D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology*; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007780:Lacticaseibacillus casei / Q000187:drug effects; D008422:Materials Testing / Q000706:statistics & numerical data; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data; D011726:Pyridinium Compounds / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000706:statistics & numerical data; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects; D013718:Tensile Strength
chemical_list: D000891:Anti-Infective Agents, Local; D017220:Dentin-Bonding Agents; D011726:Pyridinium Compounds; C089392:methacryloyloxydodecylpyridinium bromide