Molecular cloning and analysis of a HSP (heat shock protein)-like 42 kDa antigen gene of Mycoplasma hyopneumoniae.
The recombinant clone expressing the 42 kDa protein (P42) of Mycoplasma hyopneumoniae in Escherichia coli was analyzed. The 4.4 kb HindIII-Xmal DNA fragment expressing the p42 gene product encodes three ORFs: p42 and p16 in the forwarding strand, p24 in the reverse strand. Sequence comparisons revealed that p42 could be part of a p65 gene, and has 62% identities with Mycoplasma genitalium HSP70 gene and 56% identities with Bacillus subtilis dnaK gene; p16 and p24 genes share 73% and 47% identities with Erysipelothrix rhusiopathiae dnaJ gene and Pseudomonas fluorescens uvrC gene, respectively. Further analysis demonstrated that P42 is indeed a heat shock protein and the monospecific antibodies against P42 can block the growth of Mycoplasma hyopneumoniae.
Chou
S Y
SY
Department of Biology, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC.
Chung
T L
TL
Chen
R J
RJ
Ro
L H
LH
Tsui
P I
PI
Shiuan
D
D
D000595:Amino Acid Sequence; D000596:Amino Acids; D000942:Antigens, Bacterial / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics* / Q000276:immunology; D016366:Open Reading Frames / Q000235:genetics; D011993:Recombinant Fusion Proteins; D015183:Restriction Mapping; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D000596:Amino Acids; D000942:Antigens, Bacterial; C098005:HSP42 protein, S cerevisiae; D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins