Plasma and tissue thyroxine and triiodothyronine contents in sublethally stressed, aluminum-exposed brown trout (Salmo trutta).
Immature female brown trout, Salmo trutta, were exposed to pH 5.0 soft water in the presence or absence of aluminum (Al) at 12.5 micrograms liter-1 and their plasma concentrations and tissue contents of thyroxine (T4) and triiodothyronine (T3) were compared with those of a control group of trout held in pH 7.0 soft water. After 120 hr, plasma cortisol, glucose, T4, and T3 concentrations were greater in the Al-exposed trout than in trout exposed to acid conditions alone, indicating that although the Al conditions were sublethal, a significant stress response was elicited. Significant increases in liver T4 content, liver 5'-monodeiodinase activity and liver T3 content indicated increased hepatic T4 to T3 conversion in the Al-exposed trout. The T4 contents of brain, gill filaments, white muscle, heart ventricle, caudal kidney, and ovary were not significantly altered by Al exposure. The T3 content of caudal kidney and ovary were significantly lower in Al-exposed trout than in control fish in neutral water but were unchanged in the brain, gill filaments, heart ventricle, and white muscle of these trout. The present data support previous observations of increased plasma T3 concentrations in sublethally Al-exposed brown trout and indicate that at least part of the increased plasma T3 concentration is due to an increased hepatic uptake of T4 and monodeiodination to T3. However, analysis of nonhepatic tissue T3 content gave no indication of increased T3 production by these tissues.
Waring
C P
CP
Department of Biological Sciences, University of Exeter, Devon, United Kingdom.
Brown
J A
JA
D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D005260:Female; D005880:Gills / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007453:Iodide Peroxidase / Q000378:metabolism; D009132:Muscles / Q000737:chemistry; D009928:Organ Specificity; D013312:Stress, Physiological / Q000097:blood*; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood*; D014337:Trout / Q000097:blood*; D014781:Viscera / Q000737:chemistry
D014284:Triiodothyronine; D000535:Aluminum; D007453:Iodide Peroxidase; D013974:Thyroxine