Polymerase chain reaction (PCR) in the quality and safety assurance of food: detection of soya in processed meat products.
A new method for the specific and sensitive detection of soya (Glycine max) in processed meat products has been developed using polymerase chain reaction (PCR) technology. The presence of soya deoxyribonucleic acid (DNA) from several soya protein concentrates was determined with two pairs of specific oligonucleotides yielding a 414-bp (bp = base pair) fragment and an internal 118-bp fragment amplified from the soya lectin Le1 gene. The test detected DNA from textured soya protein concentrates in meat products at a level of 1% and was confirmed by a commercially available enzyme-linked immunosorbent assay (ELISA).
Meyer
R
R
Department of Food Chemistry, University of Berne, Switzerland.
Chardonnens
F
F
Hübner
P
P
Lüthy
J
J
D000818:Animals; D002417:Cattle; D002645:Chickens; D018744:DNA, Plant / Q000032:analysis; D017343:Genes, Plant; D037102:Lectins / Q000235:genetics; D008460:Meat / Q000592:standards*; D008461:Meat Products / Q000032:analysis / Q000592:standards*; D037121:Plant Lectins; D010940:Plant Proteins / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012756:Sheep; D030262:Soybean Proteins / Q000032:analysis* / Q000235:genetics; D013025:Glycine max / Q000235:genetics; D013552:Swine; D010835:Phytohemagglutinins
D018744:DNA, Plant; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins