title: Production of a T-cell clone which reacts with membrane proteins of Acholeplasma laidlawii.
abstract: The role of cellular immunity in mycoplasma infection is not completely understood. In this study, we established mycoplasma-specific T-cell clones to evaluate cellular immunity in mycoplasma infection. We developed a T-cell clone (G-10) which was stimulated with Acholeplasma laidlawii. The T-cell clone G-10, CD4+ and T-cell receptor (TCR) alpha beta- recognized the 42- and 65-kilodalton (kDa) membrane proteins of A. laidlawii and responded to A. hippikon. Hence, the application of mycoplasma-specific T cells such as G-10 in animal models may allow the assessment of cellular immune response to mycoplasma infection.
authors.affiliation: Department of Microbiology, Kurume University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kuwano
authors.forename: S
authors.initials: S
authors.lastname: Ono
authors.forename: A
authors.initials: A
authors.lastname: Akashi
authors.forename: M
authors.initials: M
authors.lastname: Ohishi
authors.forename: H
authors.initials: H
authors.lastname: Shigematsu
authors.forename: S
authors.initials: S
authors.lastname: Arai
mesh_terms: D000128:Acholeplasma laidlawii / Q000276:immunology*; D000818:Animals; D000938:Antigen-Presenting Cells; D001426:Bacterial Proteins / Q000276:immunology*; D002999:Clone Cells; D003429:Cross Reactions; D006183:H-2 Antigens; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D006183:H-2 Antigens; D008565:Membrane Proteins