title: Changes in expression of the prolactin and growth hormone gene during different reproductive stages in the pituitary gland of turkeys.
abstract: The changes in levels of prolactin (PRL) and growth hormone (GH) in plasma and the pituitary gland and their transcripts were measured in turkey hens at different physiological stages by radioimmunoassay and dot blot hybridization analysis, respectively. The levels of tPRL mRNA in the pituitary gland increased from those of the immature group to the egg-laying group, reaching a maximum during the incubation and a minimum during the moulting stages. Changes in pituitary levels of PRL and PRL mRNA followed a similar trend and consequently were highly correlated (r2 = 0.83), whereas a significant but lower correlation was observed between circulating and pituitary levels (r2 = 0.62). Less significant changes were measured for tGH mRNA, with maximum and minimum levels measured in the pituitaries of egg-laying and non-laying hens, respectively. These data suggest that although changes in concentration of PRL are correlated with the reproductive stage of the turkey hen, coordinate changes in levels of GH are not.
authors.affiliation: NEXIA Biotechnologies, Sainte-Anne-de-Bellevue, Canada.
authors.forename: C N
authors.initials: CN
authors.lastname: Karatzas
authors.forename: D
authors.initials: D
authors.lastname: Guémené
authors.forename: D
authors.initials: D
authors.lastname: Zadworny
authors.forename: U
authors.initials: U
authors.lastname: Kuhnlein
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D005260:Female; D015870:Gene Expression; D013006:Growth Hormone / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D010058:Oviposition / Q000502:physiology; D010902:Pituitary Gland / Q000378:metabolism*; D011388:Prolactin / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012098:Reproduction / Q000502:physiology*; D014422:Turkeys / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011388:Prolactin; D013006:Growth Hormone