Identification of three lineages of wild measles virus by nucleotide sequence analysis of N, P, M, F, and L genes in Japan.
The nucleotide sequences of nucleocapsid (N), phosphoprotein (P), matrix (M), fusion (F), and large protein (L) genes were partly determined for 19 wild strains of measles virus (MV) isolated over the past 10 years in Japan (nucleotide position N: 1301-1700, P: 1751-2190, M: 3571-4057, F: 6621-7210, L: 10381-11133) and also for a MV strain obtained from a patient with subacute sclerosing panencephalitis (SSPE) who had natural measles in 1980. The phylogenetic trees of these strains drawn for respective genes were very similar to each other and revealed that all the wild strains were classified chronologically into 3 subgroups, those isolated in 1984, 1984-1989, and 1990-1994. The SSPE strain was classified into the subgroup of 1984. Phylogenetic tree analyses including other strains in the world revealed that Japanese strains in 1984 were classified into a distinct lineage which might correlate with the European strains from late 1970s to mid 1980s. Japanese strains from 1984 to 1989 were almost identical to those of the United States isolated from 1989 to 1992, and Japanese strains in 1990s were related closely to some of the MV strains isolated in 1994 in the United States. Genetic recombination among the MV genes seemed not to have occurred.
Yamaguchi
S
S
Department of Paediatrics, Kitasato Institute Hospital, Tokyo, Japan.
D000293:Adolescent; D001483:Base Sequence; D005260:Female; D005787:Gene Frequency; D005814:Genes, Viral; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008457:Measles / Q000097:blood* / Q000453:epidemiology / Q000235:genetics*; D008459:Measles virus / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D019251:Nucleocapsid / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D013344:Subacute Sclerosing Panencephalitis / Q000235:genetics / Q000821:virology; D014760:Viral Fusion Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*
D010750:Phosphoproteins; D012367:RNA, Viral; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins