Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation.
We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.
Kinto
N
N
Department of Oral Pathology, School of Dentistry, Showa University, Shinagawa-ku, Tokyo, Japan.
Iwamoto
M
M
Enomoto-Iwamoto
M
M
Noji
S
S
Ohuchi
H
H
Yoshioka
H
H
Kataoka
H
H
Wada
Y
Y
Yuhao
G
G
Takahashi
H E
HE
Yoshiki
S
S
Yamaguchi
A
A
D016475:3T3 Cells; D000818:Animals; D001846:Bone Development; D002454:Cell Differentiation; D002460:Cell Line; D002642:Chick Embryo; D018920:Coculture Techniques; D004627:Embryonic Induction; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D053823:Hedgehog Proteins; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D010006:Osteoblasts / Q000166:cytology* / Q000637:transplantation; D010446:Peptide Fragments / Q000096:biosynthesis; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D013997:Time Factors; D015534:Trans-Activators; D014162:Transfection
D053823:Hedgehog Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D015534:Trans-Activators