Endogenous superoxide-like species and antioxidant activity in ocular tissues detected by luminol luminescence.
A new luminescent method was used to detect the reactive oxygen species in aqueous and vitreous humors and in homogenates of the lens and retina of laboratory rats. Superoxide-like activity per microgram protein increased in all tissues with weight of the rat, a good indicator of animal age. Superoxide dismutase, centrophenoxine, soluble vitamin E (D-alpha-Locopherol (polyethlyene glycol 1000) succinate, and N'-diphenyl-p-phenylenediamine (DPPD) reduced the luminescence. Catalase had no effect. These results are consistent with the detected species being superoxide-like.
Trevithick
J R
JR
Department of Biochemistry, Faculty of Medicine, University of Western Ontario, London, Canada.
Dzialoszynski
T
T
D000367:Age Factors; D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001835:Body Weight; D002374:Catalase; D004121:Dimethyl Sulfoxide; D005123:Eye / Q000737:chemistry*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D013482:Superoxide Dismutase; D013481:Superoxides / Q000032:analysis*
D000975:Antioxidants; D007202:Indicators and Reagents; D013481:Superoxides; D008165:Luminol; D002374:Catalase; D013482:Superoxide Dismutase; D004121:Dimethyl Sulfoxide