title: Effects of conjugated dienoic derivatives of linoleic acid and beta-carotene in modulating lymphocyte and macrophage function.
abstract: The in vitro effects of conjugated dienoic derivatives of linoleic acid (CLA) in combination with beta-carotene on lymphocyte and macrophage function was studied. Porcine blood lymphocytes and murine peritoneal macrophages were incubated with 0 (control), 1.78 x 10(-5), 3.57 x 10(-5) and 7.14 x 10(-5) M CLA and 0 (control), 10(-9), 10(-8) and 10(-7) M beta-carotene. CLA alone stimulated mitogen-induced lymphocyte proliferation, lymphocyte cytotoxic activity and macrophage bactericidal activity. In contrast, CLA inhibited interleukin-2 production by lymphocytes and suppressed the phagocytic activity of macrophages. beta-Carotene alone stimulated the cytotoxicity of lymphocytes and increased superoxide production by peritoneal macrophages. When present together, CLA and beta-carotene interacted in an additive manner to further enhance lymphocyte cytotoxicity and spontaneous lymphocyte proliferation. In addition, beta-carotene was able to negate the inhibitory action of CLA on the phagocytic activity of macrophages. Also, CLA and beta-carotene together seemed to suppress mitogen-induced lymphocyte proliferation. Therefore, CLA and beta-carotene; alone and in concert, act to modulate different aspects of cellular host defense.
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman 99164-6320, USA.
authors.forename: B P
authors.initials: BP
authors.lastname: Chew
authors.forename: T S
authors.initials: TS
authors.lastname: Wong
authors.forename: T D
authors.initials: TD
authors.lastname: Shultz
authors.forename: N S
authors.initials: NS
authors.lastname: Magnuson
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D019207:beta Carotene / Q000494:pharmacology*
chemical_list: D016588:Anticarcinogenic Agents; D007376:Interleukin-2; D008041:Linoleic Acids; D019207:beta Carotene; D013481:Superoxides; D019787:Linoleic Acid