Differentially expressed MHC class II-associated invariant chain in rat stomach pyloric mucosa with N-methyl-N-nitro-nitrosoguanidine exposure.
Administration of N-methyl-N'-nitro-N-nitrosoguanidine, a glandular stomach carcinogen, at the concentration of 100 microg/ml in drinking water for 8 days induced the appearance of a MHC class II-associated invariant chain in the target organ of stomach pyloric mucosa of male Lewis rats. The up-regulation of the MHC class II-associated invariant chain was revealed by fluorescent differential display analysis, reverse transcription-PCR, Northern blot, and histochemical staining. The appearance of MHC class II and MHC class I was also demonstrated by reverse transcription-PCR and Northern blot. The results suggest the involvement of MHC-controlled immune reactions in chemically-induced stomach carcinogenesis.
Furihata
C
C
Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Japan.
Oka
M
M
Yamamoto
M
M
Ito
T
T
Ichinose
M
M
Miki
K
K
Tatematsu
M
M
Sakaki
Y
Y
Reske
K
K
D000818:Animals; D015152:Blotting, Northern; D002273:Carcinogens; D003001:Cloning, Molecular; D005753:Gastric Mucosa / Q000187:drug effects / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D008297:Male; D008769:Methylnitronitrosoguanidine; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology; D016133:Polymerase Chain Reaction; D011708:Pylorus; D051381:Rats; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000276:immunology*
D002273:Carcinogens; D000949:Histocompatibility Antigens Class II; D008769:Methylnitronitrosoguanidine