Dose-dependent response of mature cerebrovascular axons in vivo following intracranial infusion of nerve growth factor.
The perivascular axons associated with the intradural segment of the internal carotid artery undergo a sprouting response following intracerebroventricular infusion of nerve growth factor (NGF). The objective of the present study was to determine whether a relationship exists between the number of sprouted axons and the amount of NGF infused into the ventricular system. Using regression analysis, we observed a significant log-log relationship between the dose of NGF and number of axons. No significant relationship was observed for the control (VEH) group. A significant increase in axonal number was observed following infusion of 3.0 micrograms NGF and higher when compared with VEH treatment of similar concentration. Results of this analysis suggest that a maximal response to NGF is approximated at doses of 15 micrograms or higher. These findings suggest a dose-dependent relationship between the response of mature sympathetic cerebrovascular axons in vivo and the dose of exogenous NGF.
Isaacson
L G
LG
Department of Zoology, Miami University, Oxford, OH 45056, USA.
Mareska
M
M
Nixdorf
W
W
Oris
J T
JT
D000818:Animals; D001369:Axons / Q000187:drug effects*; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D009414:Nerve Growth Factors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D009414:Nerve Growth Factors