Colchicine affects protein kinase C-induced modulation of synaptic transmission in cultured hippocampal pyramidal cells.
Spontaneous excitatory postsynaptic currents (sEPSC) through AMPA-type channels were recorded on cultured hippocampal pyramidal neurons by means of the whole-cell patch-clamp technique. The protein kinase C (PKC) agonist 4beta-phorbol 12-myristate 13-acetate (4beta-PMA) produced a long-lasting increase in sEPSC frequency not mimicked by the inactive analogue 4alpha-PM and blocked by the protein kinase inhibitor staurosporine. The 4beta-PMA-induced change in sEPSC frequency occurred without detectable change in [Ca2+]i. After treatment with the microtubule-disrupting agent colchicine, 4beta-PMA caused a small and transient increase in sEPSC frequency. It is concluded that colchicine affects the PKC-induced functional plasticity of nerve cells most likely by disturbing the axonal transport.
Bouron
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Department of Pharmacology, University of Berne, Switzerland. bouron@pki.unibe.ch
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002104:Cadmium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology*; D005071:Evoked Potentials / Q000187:drug effects; D006624:Hippocampus / Q000502:physiology*; D008870:Microtubules / Q000187:drug effects; D018408:Patch-Clamp Techniques; D011493:Protein Kinase C / Q000378:metabolism*; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D009435:Synaptic Transmission / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
D002104:Cadmium; D013779:Tetrodotoxin; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D003078:Colchicine; D002118:Calcium