Modulation of the protein kinase CK2 activity by a synthetic peptide corresponding to the N-terminus of its beta regulatory subunit.
Using a synthetic peptide corresponding to the sixteen amino-acid N-terminus of the beta subunit, the structure-activity relationship study of casein kinase 2 (CK2) was performed with regard to its previously reported property to polymerize and oligomerize in vitro. Velocity sedimentation experiments show that the peptide beta 1-16 prevents the thick filament formation and stabilizes the ring-like structure of the kinase. Furthermore, the peptide beta 1-16 stimulates the kinase activity by 3-fold toward exogenous substrates as well as the intrinsic autophosphorylation of the kinase. Such observations are in agreement with the proposed model of an activated state of CK2 when the ring-like structure is adopted. Comparison of the effects of spermine and peptide beta 1-16 on CK2 structure and activity suggests that these two activating molecules may function in a different way. Our study suggests that the N-terminal region of the beta subunit of CK2 could regulate the kinase activity by controlling the quaternary structure of the enzyme.
Valero
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CEA, Biochimie des Régulations Cellulaires Endocrines, INSERM Unit 244, DBMS, CEA Gre, Grenoble, France.
Chambaz
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EM
Cochet
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C
D000595:Amino Acid Sequence; D000818:Animals; D047390:Casein Kinase II; D002384:Catalysis; D003001:Cloning, Molecular; D004331:Drosophila melanogaster; D004789:Enzyme Activation; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000737:chemistry / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera; D013379:Substrate Specificity
D010446:Peptide Fragments; D010455:Peptides; C105173:casein kinase 2 beta (1-16); D011494:Protein Kinases; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases