[Quantitative determination of lipoprotein-X in blood serum].
Lipoprotein X is a reliable indicator of cholestasis. The present paper describes a simple method for direct measurement of lipoprotein X. Low-density lipoproteins, very low density lipoproteins, chylomicrons and abnormal lipoprotein X are precipitated from the serum by treatment with a solution of polyethylene glycol (PEG) MW 6000. Then phosphotungstic acid and PEG 6000 solution are added to the precipitate. The lipoprotein X is in the supernatant, where its level can be estimated from the content of phospholipids and cholesterol. This method permits the determination of lipoprotein X in several samples simultaneously, requires no sophisticated equipment, is sensitive, reproducible, accurate, and cheap.
Mikhova
V T
VT
V"rbanov
G P
GP
D000328:Adult; D000368:Aged; D002779:Cholestasis / Q000097:blood; D002784:Cholesterol / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D008073:Lipoprotein-X / Q000097:blood*; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood; D010772:Phosphotungstic Acid; D011092:Polyethylene Glycols; D012680:Sensitivity and Specificity; D012997:Solvents
D007202:Indicators and Reagents; D008073:Lipoprotein-X; D010743:Phospholipids; D012997:Solvents; D010772:Phosphotungstic Acid; D011092:Polyethylene Glycols; D002784:Cholesterol