Glutathione depletion in the yeast Saccharomyces cerevisiae.
The effect of chosen compounds on the total glutathione (GSH) level in stationary cultures of S. cerevisiae was compared. 1-Chloro-2,4-dinitrobenzene, 1-fluoro-2,4-dinitrobenzene, maleimide, iodacetamide and allyl alcohol (1 mM), and menadione (0.5 mM) caused an almost complete GSH depletion during several minutes. Bromobenzoic acid and chloramine T (I mM), and daunomycin (60 mu M) induced a slower GSH decrease, down to 30-70% after 60 min. Paraquat (1 mM), CuSO(4) (0.5 mM) and cadmium acetate (1 mM) decreased glutathione level down to ca 70%. Diamide (0.5 mM), phenazine methosulphate, phenylhydrazine, acetylphenylhydrazine and H(2)O(2) (1 mM), and t-butyl hydroperoxide (2 mM) did not affect total GSH during 60-min exposure. There was no clear-cut dependence between the ability of various chemicals to deplete cellular GSH and their increased toxicity to a glutathione-poor mutant.
Fortuniak
A
A
Department of Molecular Biophysics, University of Lodz, Poland. AFORT@biol.UNI.LODZ.PL
Zadzinski
R
R
Bilinski
T
T
Bartosz
G
G
D000477:Alkylating Agents / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis* / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*
D000477:Alkylating Agents; D016877:Oxidants; D005978:Glutathione