Instantaneous cloth-based enzyme immunoassay for the semi-quantitative visual determination of antibodies.
An antigen-coated cloth segment was placed onto a blotting pad. The cloth was blotted with one drop of an antibody sample, immediately washed with five drops of a washing solution, exposed to one drop of an anti-antibody-peroxidase conjugate, washed, and finally exposed to a peroxidase substrate. The colored peroxidase product was determined either visually or colorimetrically with the color intensity being correlated to the antibody concentration. This rapid assay would facilitate field serodiagnosis of human and animal diseases.
Boyd
S
S
Department of Biology and Institute of Biochemistry, Carleton University Ottawa, Ontario, Canada.
Yamazaki
H
H
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology; D003124:Colorimetry; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis*; D008070:Lipopolysaccharides / Q000276:immunology; D011113:Polymyxins; D011817:Rabbits; D015203:Reproducibility of Results; D012486:Salmonella typhimurium / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011113:Polymyxins; D006735:Horseradish Peroxidase