Effect of cyclins and Cdks on the cyclin B1 promoter activation.
To study the effect of several cyclins on cyclin B1 promoter activation, we co-transfected cyclin A. cyclin E and cyclin D1 expressing plasmids with a cyclin B1 promoter construction driving a luciferase reporter gene into NIH 3T3 cells. All three cyclins produced activation of the reporter gene, however, co-transfection of cyclin A with a Cdk2 dominant negative mutant blocks activation by cyclin A. Our results suggest that cyclin B1 activation depends on Cdk2 kinase activity associated to cyclin A. To our knowledge this is the first report showing positive effect of cyclin A, cyclin E and cyclin D1 on cyclin B1 activation and blocking of this activation by a Cdk2 dominant negative mutant.
Moro
A
A
Deutsches Krebsforschungszentrum, Heidelberg, Germany. farma@ingen.cigb.edu.cu
Zerfass
K
K
Joswig
S
S
Jansen-Duerr
P
P
D016475:3T3 Cells / Q000502:physiology; D000818:Animals; D042846:CDC2-CDC28 Kinases; D019926:Cyclin B; D056744:Cyclin B1; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000502:physiology*; D016213:Cyclins / Q000235:genetics* / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology*; D014162:Transfection
C534699:CCNB1 protein, human; C534700:Ccnb1 protein, mouse; D019926:Cyclin B; D056744:Cyclin B1; D016213:Cyclins; D008156:Luciferases; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; C495893:Cdk2 protein, mouse; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases