SOX9 is a potent activator of the chondrocyte-specific enhancer of the pro alpha1(II) collagen gene.
The identification of mutations in the SRY-related SOX9 gene in patients with campomelic dysplasia, a severe skeletal malformation syndrome, and the abundant expression of Sox9 in mouse chondroprogenitor cells and fully differentiated chondrocytes during embryonic development have suggested the hypothesis that SOX9 might play a role in chondrogenesis. Our previous experiments with the gene (Col2a1) for collagen II, an early and abundant marker of chondrocyte differentiation, identified a minimal DNA element in intron 1 which directs chondrocyte-specific expression in transgenic mice. This element is also a strong chondrocyte-specific enhancer in transient transfection experiments. We show here that Col2a1 expression is closely correlated with high levels of SOX9 RNA and protein in chondrocytes. Our experiments indicate that the minimal Col2a1 enhancer is a direct target for Sox9. Indeed, SOX9 binds to a sequence of the minimal Col2a1 enhancer that is essential for activity in chondrocytes, and SOX9 acts as a potent activator of this enhancer in cotransfection experiments in nonchondrocytic cells. Mutations in the enhancer that prevent binding of SOX9 abolish enhancer activity in chondrocytes and suppress enhancer activation by SOX9 in nonchondrocytic cells. Other SOX family members are ineffective. Expression of a truncated SOX9 protein lacking the transactivation domain but retaining DNA-binding activity interferes with enhancer activation by full-length SOX9 in fibroblasts and inhibits enhancer activity in chondrocytes. Our results strongly suggest a model whereby SOX9 is involved in the control of the cell-specific activation of COL2A1 in chondrocytes, an essential component of the differentiation program of these cells. We speculate that in campomelic dysplasia a decrease in SOX9 activity would inhibit production of collagen II, and eventually other cartilage matrix proteins, leading to major skeletal anomalies.
Lefebvre
V
V
Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Huang
W
W
Harley
V R
VR
Goodfellow
P N
PN
de Crombrugghe
B
B
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001848:Bone Diseases, Developmental / Q000235:genetics; D002356:Cartilage / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D011347:Procollagen / Q000235:genetics*; D055758:SOX9 Transcription Factor; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection
D015342:DNA Probes; D006609:High Mobility Group Proteins; D011347:Procollagen; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C527933:Sox9 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors