Immunomodulatory activity of a potent thymopentin analog: disulphide bridged beta-mercaptopropionyl-arginyl-lysyl-aspartyl-valyl-tyrosyl-cysteine amide.
The immunomodulatory activity of the disulphide bridged heptapeptide Mpa-Arg-Lys-Asp-Val-Tyr-Cys-NH2 (CTP-1), containing the thymopentin (TP5) sequence (Arg-Lys-Asp-Val-Tyr), was investigated by different immunological tests. The activity of CTP-1 was compared with the activities of two other disulphide bridged TP5 analogs: Mpa-Arg-Lys-D-Pro-Val-Tyr-Cys-NH2 (CTP-2) and Mpa-Arg-Lys-Asp-Val-Pro-Cys-NH2 (CTP3). The results obtained indicate that from the whole series of TP5 analogs CTP-1 may be of interest from the point of view of its prospective medical use. The results of autologous rosette forming cells (ARFC-test), as well as the direct examination of the influence of CTP-1 on the interleukin-1 (IL-1) production by P-388 D1 cell line evidence that the mechanism of CTP-1 action involves stimulation of IL-1 production. The results of E-rosette assay, ARFC test, and imuran test show that the activity of CTP-1 is stronger than that of TP5.
Wieczorek
Z
Z
Institute of Immunology and Experimental Therapy, Wrocław, Poland.
Zimecki
M
M
Trojnar
J
J
Siemion
I Z
IZ
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001379:Azathioprine / Q000494:pharmacology; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007941:Leukemia P388 / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D011994:Recombinant Proteins; D012397:Rosette Formation; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D016305:Thymopentin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000276:Adjuvants, Immunologic; C106419:CTP-1 peptide; C106420:CTP-2 peptide; D007375:Interleukin-1; D015850:Interleukin-6; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C106421:beta-mercaptopropionyl-arginyl--lysyl-aspartyl-valyl-prolyl-cysteine amide; D001379:Azathioprine; D016305:Thymopentin