Analysis of the 1.1-Mb human alpha/delta T-cell receptor locus with bacterial artificial chromosome clones.
Bacterial artificial chromosome (BAC) clones are effective mapping and sequencing reagents. The 1.1-Mb alpha/delta T-cell receptor locus of humans was mapped and partially sequenced with BAC clones. Seventeen BAC clones covered the 1.1-Mb alpha/delta locus, with the exception of one small gap that was expected from the coverage that a 3.7-fold BAC library is likely to provide. The end sequences of the BAC inserts could be obtained directly from the BAC DNA by sequencing with the chain terminator chemistry. Five complete BAC inserts were sequenced directly by the shotgun approach. The ends of the 17 BAC inserts were distributed evenly across the locus. By several independent criteria, the BAC clones faithfully represented the genomic DNA, with the exception of a single clone with a 68-kb deletion. These BAC features led to the proposal of a new approach to sequence the human genome.
Boysen
C
C
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Simon
M I
MI
Hood
L
L
D017104:Bacteriophage M13 / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular / Q000379:methods; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D015894:Genome, Human; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA / Q000379:methods*; D016324:Sequence Tagged Sites
D004251:DNA Transposable Elements; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta