title: Nuclear accumulation of mutant p53 protein: a possible predictor of failure of intravesical therapy in bladder cancer.
abstract: OBJECTIVE
  To investigate the expression and importance of the nuclear accumulation of p53 in superficial transitional cell carcinoma (TCC) of the bladder and its role as a predictor of response to treatment.
  
  
  METHODS
  Tumour samples from 30 patients (two women and 28 men, mean age 60.1 years, range 44-75) with pTa/pT1 tumours were assessed immunohistochemically using the Pab1801 monoclonal antibody and standard avidin-biotin peroxidase staining for p53 protein.
  
  
  RESULTS
  Tumours from six patients (20%) showed nuclear accumulation of p53; five of these patients failed intravesical therapy with bacille Calmette-Guèrin (BCG) and progressed to muscle invasive and/or metastatic disease, in contrast to six of 24 patients with no detectable nuclear oncoprotein.
  
  
  CONCLUSIONS
  The nuclear accumulation of p53 appears to be a prognostic indicator of tumour unresponsive to intravesical treatment, even with the most potent agent (BCG). Therefore, early radical treatment modalities must be seriously considered in this group of patients.
authors.affiliation: Department of Urology, SSK Istanbul Hospital, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Caliskan
authors.forename: L N
authors.initials: LN
authors.lastname: Türkeri
authors.forename: B
authors.initials: B
authors.lastname: Mansuroglu
authors.forename: G
authors.initials: G
authors.lastname: Toktas
authors.forename: B
authors.initials: B
authors.lastname: Aksoy
authors.forename: E
authors.initials: E
authors.lastname: Unlüer
authors.forename: A
authors.initials: A
authors.lastname: Akdas
mesh_terms: D000283:Administration, Intravesical; D000328:Adult; D000368:Aged; D001500:BCG Vaccine / Q000627:therapeutic use*; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000378:metabolism*; D009364:Neoplasm Recurrence, Local; D011379:Prognosis; D017211:Treatment Failure; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000628:therapy
chemical_list: D001500:BCG Vaccine; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53