Increased mitochondrial damage by lipid peroxidation in trophoblast cells of preeclamptic placentas.
Lipid peroxides and their related free radicals have been implicated in the pathogenesis of placental dysfunction in preeclampsia. Recent studies suggest that the placenta is a source of the increased lipid peroxides in the maternal circulation of women with preeclampsia. We examined intracellular localization of 4-hydroxy-2-nonenal (HNE: a major aldehydic product of lipid peroxidation)-modified proteins in human placentas by immunohistochemistry, and immunoblotting. The trophoblast layer of the chorionic villi showed intense immunoreactivity for HNE-modified proteins in 4 of 12 preeclamptic placentas, whereas no staining was observed in 12 normal placentas. Immunoblotting revealed that three immunoreactive proteins with apparent molecular mass of 110 kDa, 75 kDa, and 70 kDa were localized in the mitochondrial fraction. The present results indicate that the damage to mitochondrial proteins by lipid peroxidation by products and subsequent dysfunction of trophoblasts contribute to the pathophysiology of preeclampsia.
Morikawa
S
S
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Nagoya, Japan.
Kurauchi
O
O
Tanaka
M
M
Yoneda
M
M
Uchida
K
K
Itakura
A
A
Furugori
K
K
Mizutani
S
S
Tomoda
Y
Y
D000447:Aldehydes / Q000032:analysis; D002824:Chorionic Villi / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000032:analysis; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis; D014327:Trophoblasts / Q000737:chemistry* / Q000378:metabolism
D000447:Aldehydes; D011257:Pregnancy Proteins; D016660:NAD(P)H Dehydrogenase (Quinone); C027576:4-hydroxy-2-nonenal