title: Bacillus subtilis 168 RecR protein-DNA complexes visualized as looped structures.
abstract: The Bacillus subtilis 168 RecR protein bound to duplex DNA in the presence of ATP and divalent cations (Mg2+ and Zn2+) was visualized by electron microscopy as a nearly spherical particle. A RecR homomultimer is frequently located at the intersection of two duplex DNA strands in an interwound DNA molecule, generating DNA loops of variable length. Two individual DNA molecules bound to the same protein are seen at a very low frequency, if at all. The association of RecR with the intersection of two duplex DNA strands is more often seen in supercoiled than with relaxed or linear DNA. The RecR protein displays a slight but significant preference for negatively supercoiled over linear DNA. The minimum substrate size for RecR protein is about 150 bp in length. A possible mechanism for RecR function in DNA repair is discussed.
authors.affiliation: Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Ayora
authors.forename: A C
authors.initials: AC
authors.lastname: Stiege
authors.forename: R
authors.initials: R
authors.lastname: Lurz
authors.forename: J C
authors.initials: JC
authors.lastname: Alonso
mesh_terms: D001412:Bacillus subtilis / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000648:ultrastructure*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000648:ultrastructure*; D004278:DNA, Superhelical / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000648:ultrastructure; D003852:Deoxyribonucleoproteins / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010957:Plasmids; D011485:Protein Binding; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; D003852:Deoxyribonucleoproteins; C065831:RecR protein, Bacteria