Somatic gene transfer approaches to manipulate neural networks.
Gene transfer methods can be exploited to create somatic mosaic tissues to elucidate gene product action in cells comprising a network. This article describes the evolution of approaches beginning from herpes amplicon vectors with constitutive gene expression to herpes vectors with regulated gene expression and concludes with the development of a combined germline/somatic gene transfer method that allows for highly versatile spatial and temporal control of in vivo gene expression. Where possible examples of each approach are provided. In addition, the advantages and limitations of the approaches are discussed.
Federoff
H J
HJ
Department of Neurology, University of Rochester School of Medicine and Dentistry, NY 14642, USA. hfed@medinfo.rochester.edu
Brooks
A
A
Muhkerjee
B
B
Corden
T
T
D000818:Animals; D002478:Cells, Cultured; D003673:Defective Viruses / Q000235:genetics; D005091:Exons / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D006624:Hippocampus / Q000166:cytology; D009030:Mosaicism / Q000235:genetics; D009415:Nerve Net; D009474:Neurons / Q000378:metabolism / Q000821:virology; D011401:Promoter Regions, Genetic; D051381:Rats; D018092:Kainic Acid Receptors / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000502:physiology; D018139:Simplexvirus / Q000235:genetics; D009435:Synaptic Transmission; D014162:Transfection / Q000379:methods*
D018092:Kainic Acid Receptors; D011993:Recombinant Fusion Proteins