Carbohydrate induced modulation of cell membrane. VI. Binding of exogenous lectin induces susceptibility of erythrocytes to free radical damage: a spin label study.
The oxidation of erythrocyte membrane has been widely used as a model to study the damage of biomembranes by free radicals. Whether binding of lectin to erythrocytes has any effect on peroxidant injury has never been studied. This study reports for the first time that crosslinking of erythrocyte surface glycoprotein by an exogenous lectin significantly enhances the susceptibility to membrane damage by free radicals, as evidenced by the increase in membrane fluidity measured by EPR using spin label and the increase in the amount of oxyhemoglobin liberated due to cell lysis.
Hajela
K
K
School of Life Sciences, Devi Ahilya Vishwavidyalaya, Vigyan Bhawan, Indore, India.
Pande
A H
AH
Sumati
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D006384:Hemagglutination; D006461:Hemolysis; D037102:Lectins / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D008560:Membrane Fluidity; D008562:Membrane Glycoproteins / Q000378:metabolism; D037121:Plant Lectins; D011748:Pyrogallol / Q000494:pharmacology; D011817:Rabbits; D013113:Spin Labels; D013481:Superoxides / Q000494:pharmacology*
D037102:Lectins; D008562:Membrane Glycoproteins; D037121:Plant Lectins; D013113:Spin Labels; C033619:lentil lectin; D011748:Pyrogallol; D013481:Superoxides