Photo-irradiation improved functional preservation of the isolated rat heart.
OBJECTIVE
Photo-irradiation causes a variety of effects in different cells and tissues. We hypothesized that photo-irradiation may improve cardiac preservation based on these observations.


METHODS
For pre-storage treatment (Pre), the heart in an anesthetized open-chest rat was irradiated using an Argon-dye laser with a wavelength of 660 nm at a fluence of 16.8 J/cm2 or sham-operated. The heart was excised, perfused with Krebs-Henseleit Buffer, cardioplegically arrested, and stored by immersion at 0 degree C for 18 hrs. Functional recovery was evaluated by working reperfusion for 30 min. For post-storage treatment (Post), the isolated hearts were stored for 18 hrs at 0 degree C; laser irradiation at a fluence of 36 J/cm2 was administered during working reperfusion. Hearts which did not receive irradiation during reperfusion served as control. Furthermore, isolated cardiomyocytes were used to study laser effect on cellular ATP content, catalase activity, and nitric oxide (NO) release.


RESULTS
Both Pre and Post groups showed significant improvement in recovery of aortic flow, cardiac output, and work compared to the corresponding control groups (P < 0.05). Combined Pre/Post laser treatment did not improve function. Investigation using isolated rat cardiomyocytes found that both end-storage ATP and end-reperfusion catalase activity in the laser-treated group were significantly higher than those in the untreated cells (P < 0.05). NO release increased by 15% in the laser group after 18 hrs of 37 degrees C incubation.


CONCLUSIONS
Photo-irradiation improves functional recovery of the cold-stored rat heart possibly via conservation of ATP and antioxidant enzyme activity.
Zhu
Q
Q
Department of Surgery, University of Rochester, New York 14642, USA.
Yu
W
W
Yang
X
X
Hicks
G L
GL
Lanzafame
R J
RJ
Wang
T
T
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure; D002302:Cardiac Output; D002374:Catalase / Q000378:metabolism; D003326:Coronary Circulation; D006321:Heart / Q000502:physiology / Q000528:radiation effects*; D066298:In Vitro Techniques; D007834:Lasers; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009926:Organ Preservation; D051381:Rats; D017207:Rats, Sprague-Dawley
D009569:Nitric Oxide; D000255:Adenosine Triphosphate; D002374:Catalase