title: Flow injection fluorimetric determination of ascorbic acid based on its photooxidation by thionine blue.
abstract: The photooxidation of ascorbic acid sensitized by Thionine Blue was studied. The Leucothionine Blue formed during the reaction is highly fluorescent. A flow injection method using merging zones is proposed for the determination of ascorbic acid over a concentration range from 8 x 10(-7) to 5 x 10(-5) mol l-1 with a throughout of 80 samples per h. The method was used for the simple and rapid determination of ascorbic acid in pharmaceuticals, fruit juices and soft drinks.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Pérez-Ruiz
authors.forename: C
authors.initials: C
authors.lastname: Martínez-Lozano
authors.forename: V
authors.initials: V
authors.lastname: Tomás
authors.forename: C
authors.initials: C
authors.lastname: Sidrach
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D017022:Flow Injection Analysis; D008027:Light; D010084:Oxidation-Reduction; D010640:Phenothiazines / Q000737:chemistry
chemical_list: D010640:Phenothiazines; D001205:Ascorbic Acid; C009469:thionine