title: A ryanodine-sensitive calcium store in ascidian eggs monitored by whole-cell patch-clamp recordings.
abstract: Using whole cell patch clamp recordings on unfertilized eggs of the ascidian Ciona intestinalis, we are able to detect ryanodine receptors within the oocytes. Our approach is based on measurements of the voltage-activated inward calcium currents. Two types of Ca2+ currents have been described on the oocyte membrane of Ciona: a low threshold slowly activating current, and a high threshold faster one. We show here that caffeine induces a decrease in the intensity of the Ca2+ currents, when applied either externally or internally from the mouth of a patch pipette. Caffeine application mimics fertilization which transiently decreases the high threshold Ca2+ current density during density during the first meiotic cycle. Ryanodine (> 1 nM) has an effect similar to caffeine. This partial decrease in Ca2+ current density elicited by caffeine or ryanodine is prevented by intracellular application of the calcium chelator BAPTA, then imputable to calcium release. In summary, the depolarization-induced Ca2+ current intensity allows monitoring of an intracellular calcium store which is sensitive to low concentrations of ryanodine in Ciona oocytes. Further identification of a ryanodine receptor was obtained by immunological staining with antibodies against mammalian skeletal muscle ryanodine receptor. Ryanodine receptors were asymmetrically localized in the cortex of Ciona eggs. We discuss the methodological relevance of our patch-clamp approach, in connection with the possible biological role of such a ryanodine receptor in the early stages of development.
authors.affiliation: Laboratoire de Biophysique Moléculaire et Cellulaire, DSV/DBMS, Grenoble, France.
authors.forename: C
authors.initials: C
authors.lastname: Arnoult
authors.forename: M
authors.initials: M
authors.lastname: Albrieux
authors.forename: A F
authors.initials: AF
authors.lastname: Antoine
authors.forename: D
authors.initials: D
authors.lastname: Grunwald
authors.forename: I
authors.initials: I
authors.lastname: Marty
authors.forename: M
authors.initials: M
authors.lastname: Villaz
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000032:analysis* / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D002938:Ciona intestinalis; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D005306:Fertilization; D009124:Muscle Proteins / Q000032:analysis* / Q000378:metabolism; D009865:Oocytes / Q000737:chemistry / Q000502:physiology*; D018408:Patch-Clamp Techniques; D012433:Ryanodine / Q000494:pharmacology*; D019837:Ryanodine Receptor Calcium Release Channel
chemical_list: D015220:Calcium Channels; D002614:Chelating Agents; D009124:Muscle Proteins; D019837:Ryanodine Receptor Calcium Release Channel; D012433:Ryanodine; D002110:Caffeine; D004533:Egtazic Acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium