The base analog 6-N-hydroxylaminopurine (HAP) mutagenesis is dependent on the integrity of the uvsE, uvsF and uvsB genes in Aspergillus nidulans.
Most of the available data in lower eukaryotes are consistent with the idea that base analogs-induced mutagenesis is due to the mis-pairing properties of these compounds, which, in turn, is due to a shift in the tautomeric equilibrium of the molecule. A tautomeric shift may in fact lead to mismatches which, at least in Escherichia coli, can be repaired by genes involved in the post-replicative mismatch repair whose activity is necessary to control spontaneous mutagenesis. In filamentous fungi, such as Aspergillus nidulans, nothing is known about the repair of base pairing mistakes after base analogs treatment. For this reason, we have decided to screen UV-sensitive Aspergillus nidulans mutants for their mutagenic response to 6-N-hydroxylaminopurine (HAP). We have shown that three mutations (uvsB, uvsC and uvsE), which enhance the UV-sensitivity of germinating conidia, cause a lower mutagenic response to HAP. On the other hand, the uvsH mutation, has no effect on HAP-induced mutagenesis.
Babudri
N
N
Istituto di Anatomia Comparata, Università di Perugia, Italy.
Morpurgo
G
G
Marini
A
A
Prantera
G
G
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development; D015169:Colony Count, Microbial; D004260:DNA Repair; D004271:DNA, Fungal / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins; D016296:Mutagenesis; D014466:Ultraviolet Rays / Q000009:adverse effects
D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C095234:UvsH protein, Aspergillus nidulans; C080924:uvs-2 protein, Neurospora crassa; C032610:6-N-hydroxylaminopurine; D000225:Adenine