Neutrophils from burn patients are unable to increase the expression of CD11b/CD18 in response to inflammatory stimuli.
Neutrophils (PMNs) from patients with thermal injury are dysfunctional for the CD11b/CD18-dependent functions of diapedesis, chemotaxis, and phagocytosis. The expression of CD11b/CD18 on normal PMNs is increased after an inflammatory stimulus. We proposed that CD11b/CD18 expression on burn patient PMNs would respond abnormally to inflammatory stimuli. PMNs were obtained from nonseptic burn patients during the second week after thermal injury. PMNs from burn patients incubated with lipopolysaccharide (LPS), N-formyl-methionyl-leucyl-phenylalanine, phorbol myristate acetate, or A23187 did not increase the expression of CD11b/CD18 to the same degree exhibited by normal PMNs. This inability to increase CD11b/CD18 was not due to differences in CD14 receptor expression, LPS binding, or factors present in the serum of burn patients. The upregulation of CD35 also was decreased on burn patient PMNs. Western blot analysis revealed decreased quantities of CD11b protein in burn patient PMNs compared with normal control PMNs. The deficiency in CD11b/CD18 expression after inflammatory stimuli may explain some of the abnormalities observed in burn PMN function.
Rodeberg
D A
DA
Shriners Burns Institute, Department of Surgery, University of Cincinnati College of Medicine, Ohio 45229-3095, USA.
Bass
R C
RC
Alexander
J W
JW
Warden
G D
GD
Babcock
G F
GF
D002056:Burns / Q000097:blood*; D018821:CD18 Antigens / Q000097:blood*; D000001:Calcimycin / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology; D016177:Macrophage-1 Antigen / Q000097:blood*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011946:Receptors, Antigen / Q000378:metabolism; D017463:Receptors, Complement 3b / Q000097:blood; D013268:Stimulation, Chemical; D015854:Up-Regulation / Q000187:drug effects
D018821:CD18 Antigens; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen; D011946:Receptors, Antigen; D017463:Receptors, Complement 3b; D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine