title: Gap junction localization and connexin expression in cytochemically identified endothelial cells of arterial tissue.
abstract: Vascular endothelial cells interact with one another via gap junctions, but information on the precise connexin make-up of endothelial gap junctions in intact arterial tissue is limited. One factor contributing to this lack of information is that standard immunocytochemical methodologies applied to arterial sections do not readily permit unequivocal localization of connexin immunolabeling to endothelium. Here we introduce a method for multiple labeling with specific endothelial cell markers and one or more connexin-specific antibodies which overcomes this limitation. Applying this method to localize connexins 43, 40, and 37 by confocal microscopy, we show that the three connexin types have quite distinctive labeling patterns in different vessels. Whereas endothelial cells of rat aorta and coronary artery characteristically show extensive, prominent connexin40, and heterogeneous scattered connexin37, the former, unlike the latter, also has abundant connexin43. The relative lack of connexin43 in coronary artery endothelium was confirmed in both rat and human using three alternative antibodies. In the aorta, connexins43 and 40 commonly co-localize to the same junctional plaque. Even within a given type of endothelium, zonal variation in connexin expression was apparent. In rat endocardium, a zone just below the mitral valve region is marked by expression of greater quantities of connexin43 than surrounding areas. These results are consistent with the idea that differential expression of connexins may contribute to modulation of endothelial gap junction function in different segments and subzones of the arterial system.
authors.affiliation: Imperial College School of Medicine at National Heart and Lung Institute, London, United Kingdom.
authors.forename: H I
authors.initials: HI
authors.lastname: Yeh
authors.forename: E
authors.initials: E
authors.lastname: Dupont
authors.forename: S
authors.initials: S
authors.lastname: Coppen
authors.forename: S
authors.initials: S
authors.lastname: Rothery
authors.forename: N J
authors.initials: NJ
authors.lastname: Severs
mesh_terms: D000818:Animals; D001158:Arteries / Q000166:cytology / Q000378:metabolism*; D017630:Connexins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D017629:Gap Junctions / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017630:Connexins