Effects of benzene on splenic, thymic, and femoral lymphocytes in mice.
Chronic exposure to high concentrations of benzene, primarily by inhalation, can affect the function of the human immune system. Limited data are available on the immunotoxic effects of low concentrations of benzene. This study evaluated the effects of 1, 5, 10, 100, and 200 ppm benzene on lymphocytes in mice exposed by inhalation for up to 8 weeks. Exposure to 100 or 200 ppm benzene induced rapid and persistent reductions in femoral B-, splenic T- and B-, and thymic T-lymphocytes. The percentage of femoral B-lymphocytes and thymic T-lymphocytes in apoptosis was increased 6- to 15-fold by 200 ppm benzene compared to controls. Replication of femoral B-lymphocytes was increased during the exposure period in the bone marrow as a compensation for the lymphocyte loss induced by 100 and 200 ppm benzene. Exposure of mice to 10 ppm benzene or less did not have a statistically significant effect on numbers or replication of the lymphocyte populations evaluated. A reduced number of splenic B-lymphocytes after 2 weeks of exposure to benzene appeared to be the most sensitive end point and time point for evaluating benzene cytotoxicity in this study.
Farris
G M
GM
Chemical Industry Institute of Toxicology, Research Triangle Park, NC 27709, USA. gmfarris@mailzone.com
Robinson
S N
SN
Wong
B A
BA
Wong
V A
VA
Hahn
W P
WP
Shah
R
R
D000280:Administration, Inhalation; D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D001554:Benzene / Q000008:administration & dosage / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine / Q000737:chemistry; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005269:Femur / Q000166:cytology / Q000187:drug effects; D018655:Lymphocyte Count / Q000187:drug effects; D008297:Male; D051379:Mice; D016196:S Phase / Q000187:drug effects; D012997:Solvents / Q000008:administration & dosage / Q000633:toxicity*; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects
D002273:Carcinogens; D012997:Solvents; D001973:Bromodeoxyuridine; D001554:Benzene