An intron capture strategy used to identify and map a lysyl oxidase-like gene on chromosome 9 in the mouse.
An intron capture strategy involving use of polymerase chain reaction was used to identify and map the mouse homologue of a human lysyl oxidase-like gene (LOXL). Oligonucleotides complementary to conserved domains within exons 4 and 5 of the human lysyl oxidase-like gene were used to amplify the corresponding segment from mouse genomic DNA. Sequencing of the resulting mouse DNA fragment of approximately 1 kb revealed that the exon sequences at the ends of the amplified fragment are highly homologous (90% nucleotide identity) to exons 4 and 5 of the human lysyl oxidase-like gene. An AluI restriction site polymorphism within intron 4 was used to map the mouse lysyl oxidase-like gene (Loxl) to mouse Chromosome 9 in a region that shares linkage conservation with human chromosome 15q24, to which the LOXL was recently mapped.
Wydner
K S
KS
Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855-1059, USA.
Kim
Y
Y
Csiszar
K
K
Boyd
C D
CD
Passmore
H C
HC
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D005091:Exons / Q000235:genetics; D005838:Genotype; D006801:Humans; D007438:Introns / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D008249:Protein-Lysine 6-Oxidase