Preparation of functionally active [35S]leptin for mapping its receptors in the brain.
Guan
X M
XM
Department of Biochemistry and Physiology, Merck Research Laboratories, Rahway, New Jersey 07065, USA.
Yu
H
H
Van der Ploeg
L H
LH
Smith
R G
RG
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D020738:Leptin; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011869:Radioligand Assay / Q000379:methods*; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D013462:Sulfur Radioisotopes; D014982:Xenopus laevis
D002352:Carrier Proteins; D020738:Leptin; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D013462:Sulfur Radioisotopes; C515447:leptin receptor, mouse