The influence of sintering temperature on the proliferation of fibroblastic cells in contact with HA-bioceramics.
HA-ceramics used in human surgery as osteoconductive surfaces show a great variety of characteristics. Certain characteristics such as grain size, porosity, and surface area, are controlled by the sintering temperature of the slurry. We grew L-929 fibroblast cells on HA-ceramic disks that had been sintered at different temperatures ranging from 850 degrees-1350 degrees C. The cell line growth rate was lower on ceramic disks than on the culture-grade polystyrene used as a negative control. Cell growth correlated with the ceramic sintering temperature although no significant difference in the cell adhesion to the different ceramics was shown. Growth rate on ceramics sintered at low temperatures (850 degrees and 950 degrees C) was negative whereas it was positive on disks sintered at higher temperatures. When the cells were separated from the disks by a polycarbonate membrane, the growth rate was negative on those membranes in contact with low-temperature sintered disks and positive on the high-temperature sintered disks. The calcium and phosphorus concentration in the culture medium in contact with ceramics sintered below 1050 degrees C decreased during the culture period. Ceramics sintered between 1100 degrees and 1250 degrees C brought about an increase in Ca and P concentrations while ceramics sintered at higher temperatures did not induce any changes. SEM examination of the 850 degrees and 1200 degrees C sintered ceramics showed that the 850 degrees C sintered ceramics consisted of small grains with pores between them and the 1200 degrees C sintered ceramics were made of larger grains without any visible pores, thereby decreasing the surface of material in contact with the culture medium. This difference in surface area was confirmed by the fact that the amount of albumin absorbed onto the ceramic was dependent on the sintering temperature. In conclusion, the modification of the culture medium brought about by high-surfaced ceramics could influence the growth of cells with which such ceramics come in contact.
Frayssinet
P
P
Bioland, Toulouse, France.
Rouquet
N
N
Fages
J
J
Durand
M
M
Vidalain
P O
PO
Bonel
G
G
D000818:Animals; D018786:Bone Substitutes; D002118:Calcium / Q000032:analysis; D002130:Calcium Phosphates; D002455:Cell Division; D002516:Ceramics; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007700:Kinetics; D007739:L Cells; D008422:Materials Testing / Q000379:methods; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010710:Phosphates / Q000032:analysis; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014961:X-Ray Diffraction
D018786:Bone Substitutes; D002130:Calcium Phosphates; D010710:Phosphates; D002118:Calcium