Conditioned media obtained from a human mastocytosis cell strain induce mast cells expressing chymase but not tryptase from human progenitors.
Human mast cells (MC) were derived from umbilical cord blood and bone marrow progenitors cultured in the presence of a conditioned medium from a human mastocytosis cell strain and recombinant human kit ligand (rhKL). KL induced MC of predominantly two immunophenotypes, MC(T) and MC(TC). In contrast, the conditioned medium induced MC subtypes MC(TC) and a third subtype, MC(C), positive for chymase but negative for tryptase. This study clearly demonstrates that a third type of MC, MC(C), can be induced in vitro from normal human progenitors.
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School of Medicine, University of New South Wales and Department of Immunology, Allergy and Infectious Disease, St. George Hospital, Kogarah, Australia.
Krilis
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D001854:Bone Marrow Cells; D002478:Cells, Cultured; D053818:Chymases; D017077:Culture Media, Conditioned; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D006801:Humans; D008407:Mast Cells / Q000201:enzymology*; D008415:Mastocytosis / Q000503:physiopathology*; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D019089:Stem Cell Factor / Q000494:pharmacology; D053802:Tryptases
D017077:Culture Media, Conditioned; D019089:Stem Cell Factor; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases