Growth and cyclosporin A production by an indigenously isolated strain of Tolypocladium inflatum.
A fungal culture isolated from a local soil sample which showed antifungal activity and produced cyclosporin A, was identified as Tolypocladium inflatum. The culture grew best in a medium containing 1% maltose (pH 5-6) when inoculated with a one-day-old inoculum at 2% (V/V) concentration. Under batch fermentation conditions, growth and cyclosporin A production were better in complex media (24.6 g biomass and 205 mg cyclosporin A per liter) in comparison with synthetic media (6.84 g biomass and 35 mg cyclosporin A per liter). While addition of peptone increased culture growth (high biomass yield), supplementation with casein acid hydrolyzate favored cyclosporin A production.
Balakrishnan
K
K
Biotechnology Unit, Council of Scientific & Industrial Research, Trivandrum, India.
Pandey
A
A
D003470:Culture Media; D016572:Cyclosporine / Q000096:biosynthesis*; D005285:Fermentation; D003904:Mitosporic Fungi / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology
D003470:Culture Media; D016572:Cyclosporine