Monoclonal antibodies against the common polysaccharide of Streptococcus agalactiae.
A monoclonal antibody giving a dominant reaction with the group-specific polysaccharide of streptococcus group B in an ELISA test has been developed. The purified polysaccharide exhibited a high positivity with reference anti B streptococcal antiserum in the ELISA test. Cross-tests of antibodies with other groups of streptococci provided a minimum cross-reaction only in the case of G streptococci. Monoclonal antibodies were prepared using Streptococcus agalactiae S 589 MT strain isolated from a case of bovine mastitis which does not express Ia, Ib, II, III, IV and V type antigens, nor C, R and X protein antigens.
Rosocha
J
J
University of Veterinary Medicine, Department of Microbiology and Immunology, Kosice, Slovakia.
Mikula
I
I
Kollárová
Z
Z
Holoda
E
E
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002417:Cattle; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008414:Mastitis, Bovine / Q000382:microbiology; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D013292:Streptococcus agalactiae / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity
D000911:Antibodies, Monoclonal; D011135:Polysaccharides, Bacterial