Nucleotide and calcium-induced conformational changes in histone H1.
The principal constituents of chromatin, histone H1 (H1) and the nucleosome have essential roles in regulation of eukaryotic gene expression. However, mechanisms for the H1-dependent inactivation and for the ATP-dependent chromatin remodeling upon activation are largely unelucidated. Using circular dichroism (CD) analysis we show that ATP and other nucleotides and Ca2+ induce structural changes in H1. ATP and Ca2+ also induce changes when H1 is interacting with DNA, and the changes in H1 are accompanied by alterations in its DNA interaction. These results suggest that nucleotide and Ca2+ binding may be important for H1-mediated chromatin changes.
Tarkka
T
T
Department of Applied Cell and Molecular Biology, University of Umeå, Sweden.
Oikarinen
J
J
Grundström
T
T
D000227:Adenine Nucleotides / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D006657:Histones / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D011487:Protein Conformation; D017510:Protein Folding; D051381:Rats
D000227:Adenine Nucleotides; D006657:Histones; D010710:Phosphates; D004247:DNA; D002118:Calcium