The tetramethylammonium chloride (TMAC) method for screening cDNA libraries with highly degenerate oligonucleotide probes obtained by reverse translation of amino acid sequences.
Honoré
B
B
Danish Center for Human Genome Research, Aarhus, Denmark.
Madsen
P
P
D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary; D015723:Gene Library; D006801:Humans; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods; D011506:Proteins / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds
D018076:DNA, Complementary; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D010455:Peptides; D011506:Proteins; D000644:Quaternary Ammonium Compounds; C027917:tetramethylammonium