Isolation of a novel human cDNA (rhoHP1) homologous to rho genes.
A novel full-length cDNA showing homology with rho genes was isolated from a human placenta cDNA library. Sequencing of a total of 1086 nucleotides of this clone revealed an open reading frame of 630 nucleotides (210 amino acids). In view of its degree of homology to members of the Rho family of molecules (50-54% identical amino acids, 60-63% identical nucleotides within the coding region), the predicted product was designated RhoHP1(Rho-related protein HP1). Northern analysis indicated that a message about 1.2-kb long is expressed in human heart, placenta, liver, skeletal muscle, and pancreas and, with weaker intensity, in several other tissues.
Shimizu
F
F
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan. shimizu@otsuka.genome.ad.jp
Watanabe
T K
TK
Okuno
S
S
Omori
Y
Y
Fujiwara
T
T
Takahashi
E
E
Nakamura
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D019204:GTP-Binding Proteins / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D020741:rho GTP-Binding Proteins
D018076:DNA, Complementary; D012333:RNA, Messenger; C105243:RHOD protein, human; D019204:GTP-Binding Proteins; D020741:rho GTP-Binding Proteins