Immunohistochemical colocalization of GTP cyclohydrolase I in the nigrostriatal system with tyrosine hydroxylase.
Immunohistochemical colocalization of GTP cyclohydrolase I (GCH) in the mouse nigrostriatal system with tyrosine hydroxylase or aromatic L-amino acid decarboxylase in the somata and terminals of GCH-positive catecholaminergic neurons are proved for the first time by a double-labeling immunofluorescence method with a confocal laser-scanning microscope. GCH-immunoreactive somata in the mouse substantia nigra have synaptic contacts with monoaminergic and non-monoaminergic terminals.
Nagatsu
I
I
Department of Anatomy, School of Medicine, Fujita Health University, Aichi, Japan.
Arai
R
R
Sakai
M
M
Yamawaki
Y
Y
Takeuchi
T
T
Karasawa
N
N
Nagatsu
T
T
D000818:Animals; D006136:GTP Cyclohydrolase / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D013378:Substantia Nigra / Q000201:enzymology* / Q000648:ultrastructure; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D014793:Visual Cortex / Q000201:enzymology* / Q000648:ultrastructure
D014446:Tyrosine 3-Monooxygenase; D006136:GTP Cyclohydrolase