An efficient method that reveals both the dendrites and the soma mosaics of retinal ganglion cells.
A method of using neurobiotin to stain both the dendrites and the soma mosaics of retinal ganglion cells in fresh retinae is described. This method is simple to use and efficient in revealing morphological details for a large number of retinal ganglion cells. It has five advantages over currently available staining methods. (1) It stains all ganglion cells in the whole retina or in a selected retinal area, permitting ganglion cell distributions across the retina to be obtained. (2) It reveals cell dendrites in great detail, especially in regions outside the area centralis. The dendritic field mosaics and, therefore the dendritic field coverage factors, of different ganglion cell types across the whole retina can be obtained easily. (3) It works reliably, efficiently, and does not require the expensive set-up or the pains-taking work needed when staining cells through intracellular injection. (4) It works under both in vivo and in vitro settings, permitting the use of retinae from animals sacrificed for other purposes and the use of postmortem human retinae. (5) The end product of the visualization process is optically dark and electron dense, permitting specimens to be examined under both light and electron microscopes.
Zhan
X J
XJ
Neuroscience Institute and Biomedical Engineering Department, Northwestern University, Evanston, IL 60208, USA.
Troy
J B
JB
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives*; D002415:Cats; D048429:Cell Size; D003712:Dendrites / Q000502:physiology*; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase; D007546:Isoquinolines; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods
D005456:Fluorescent Dyes; D007546:Isoquinolines; C070614:neurobiotin; D001710:Biotin; C017475:lucifer yellow; D006735:Horseradish Peroxidase