Dendritic cells enhance growth and differentiation of CD40-activated B lymphocytes.
After antigen capture, dendritic cells (DC) migrate into T cell-rich areas of secondary lymphoid organs, where they induce T cell activation, that subsequently drives B cell activation. Here, we investigate whether DC, generated in vitro, can directly modulate B cell responses, using CD40L-transfected L cells as surrogate activated T cells. DC, through the production of soluble mediators, stimulated by 3- to 6-fold the proliferation and subsequent recovery of B cells. Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells). In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM. Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.
Dubois
B
B
Schering Plough, Laboratory for Immunological Research, Dardilly, France.
Vanbervliet
B
B
Fayette
J
J
Massacrier
C
C
Van Kooten
C
C
Brière
F
F
Banchereau
J
J
Caux
C
C
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D019013:CD40 Antigens / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002450:Cell Communication; D002454:Cell Differentiation; D002458:Cell Fractionation; D002460:Cell Line; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007156:Immunologic Memory; D007739:L Cells; D007801:Langerhans Cells / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D009000:Monocytes / Q000276:immunology; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology
D019013:CD40 Antigens; D007136:Immunoglobulins