cDNA cloning and expression of a class II acidic chitinase from sweet orange.
A citrus cDNA encoding a class II acidic chitinase was isolated from a nonembryogenic cell line of sweet orange using the tobacco cDNA clone PROB3. Northern blot analysis revealed that the corresponding mRNA is expressed in young, green bark but not in leaves, roots, or flavedo.
Nairn
C J
CJ
U.S. Horticulture Research Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Orlando, FL 32803, USA.
Niedz
R P
RP
Hearn
C J
CJ
Osswald
W F
WF
Mayer
R T
RT
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D002688:Chitinases / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005638:Fruit / Q000201:enzymology / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant; D002688:Chitinases