title: Reversible antisense inhibition of Shaker-like Kv1.1 potassium channel expression impairs associative memory in mouse and rat.
abstract: Long-term memory is thought to be subserved by functional remodeling of neuronal circuits. Changes in the weights of existing synapses in networks might depend on voltage-gated potassium currents. We therefore studied the physiological role of potassium channels in memory, concentrating on the Shaker-like Kv1.1, a late rectifying potassium channel that is highly localized within dendrites of hippocampal CA3 pyramidal and dentate gyrus granular cells. Repeated intracerebroventricular injection of antisense oligodeoxyribonucleotide to Kv1.1 reduces expression of its particular intracellular mRNA target, decreases late rectifying K+ current(s) in dentate granule cells, and impairs memory but not other motor or sensory behaviors, in two different learning paradigms, mouse passive avoidance and rat spatial memory. The latter, hippocampal-dependent memory loss occurred in the absence of long-term potentiation changes recorded both from the dentate gyrus or CA1. The specificity of the reversible antisense targeting of mRNA in adult animal brains may avoid irreversible developmental and genetic background effects that accompany transgenic "knockouts".
authors.affiliation: Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: N
authors.initials: N
authors.lastname: Meiri
authors.forename: C
authors.initials: C
authors.lastname: Ghelardini
authors.forename: G
authors.initials: G
authors.lastname: Tesco
authors.forename: N
authors.initials: N
authors.lastname: Galeotti
authors.forename: D
authors.initials: D
authors.lastname: Dahl
authors.forename: D
authors.initials: D
authors.lastname: Tomsic
authors.forename: S
authors.initials: S
authors.lastname: Cavallaro
authors.forename: A
authors.initials: A
authors.lastname: Quattrone
authors.forename: S
authors.initials: S
authors.lastname: Capaccioli
authors.forename: A
authors.initials: A
authors.lastname: Bartolini
authors.forename: D L
authors.initials: DL
authors.lastname: Alkon
mesh_terms: D000818:Animals; D001245:Association Learning / Q000187:drug effects / Q000502:physiology*; D018891:Dentate Gyrus / Q000166:cytology / Q000502:physiology; D006624:Hippocampus / Q000502:physiology; D007276:Injections, Intraventricular; D051662:Kv1.1 Potassium Channel; D017774:Long-Term Potentiation; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008845:Microinjections; D009043:Motor Activity / Q000187:drug effects; D009434:Neural Pathways; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D015221:Potassium Channels / Q000235:genetics / Q000502:physiology*; D024642:Potassium Channels, Voltage-Gated; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: C496947:Kcna1 protein, mouse; D016376:Oligonucleotides, Antisense; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D051662:Kv1.1 Potassium Channel