A new data-acquisition system for the measurement of the net water flux across epithelia.
We describe a computer-based data-acquisition system designed for the measurement of net water flux across epithelia. It is based on the detection of a liquid meniscus position in a capillary tube using an electro-optical device. Then the data are digitized, transferred to the computer main memory and saved in a file. The advantages of the system are: (a) to measure net water transfer with an accuracy of 50 nl; (b) to switch from absorptive to secretory conditions in real time; (c) data are directly digitized to be analytically, graphically and statistically treated; (d) the specially developed software allows change of time intervals for data acquirement and the scale in graphic events; (e) the software also allows recall to the monitor screen of graphics from previous tests or to print them on paper; (f) a simple mechanical structure, saving maintenance and time; and (g) high reliability.
Dorr
R A
RA
Facultad de Medicina (UBA), Departamento de Fisiología, Buenos Aires, Argentina. rdorr@biomem.fmed.uba.ar
Kierbel
A
A
Vera
J
J
Parisi
M
M
D000818:Animals; D001834:Body Water / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D003205:Computing Methodologies; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D012984:Software