Use of high-performance liquid chromatography with photodiode-array UV detection for the creation of a 600-compound library. Application to forensic toxicology.
A high-performance liquid chromatographic method is described with photodiode array detection for systematic toxicological analysis in human blood and urine. After a single step liquid-liquid extraction using Toxi-Tube A, drugs are analyzed with a multi step gradient (phosphate pH 3.8-acetonitrile) on a Symmetry C8 5-microns column (250 mm x 4.6 mm I.D.) (Waters), operated at 30 degrees C. The flow-rate is varied during the run from 1 ml/min to 1.5 ml/min. Full UV spectra are recorded on-line during the 28 min chromatographic run. Enhanced performances for drug detection are obtained with this method due to (a) reduction of peak-tailing for basic drugs, (b) lower identification limits, (c) more stable retention times and (d) larger number of referenced drugs (684 spectrum registered). Application of real samples has been demonstrated.
Gaillard
Y
Y
Laboratoire d'Expertises TOXLAB, Paris, France.
Pépin
G
G
D000328:Adult; D001774:Blood Chemical Analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005554:Forensic Medicine; D006801:Humans; D007993:Libraries, Medical; D008297:Male; D013056:Spectrophotometry, Ultraviolet; D015813:Substance Abuse Detection / Q000379:methods; D014116:Toxicology / Q000379:methods*; D014556:Urine / Q000737:chemistry