title: Comparative in vitro sensitivity of human cholangiocarcinoma and colon adenocarcinoma cells to anticancer agents.
abstract: We comparatively investigated the sensitivity of a human cholangiocarcinoma cell line (SG231) and an adenocarcinoma cell line (WiDr) to mitoxantrone (MX), taxol (TX), mitomycin C (MMC), doxorubicin (DX), cisplatin (CDDP) and 5-fluorouracil (5FU) by the sulforhodamine B assay. The lower susceptibility of SG231 to SFU, to CDDP, to DX and to MMC than WiDr was observed, whereas the sensitivity of the two cell lines to MX and TX was similar. We also investigated the ability of a chemical modulator, lonidomine (LND), and hyperthermia to enhance the cytotoxic activity of the different drugs in the SG231 cell line. No potentiation of MX or CDDP activity was observed after a 2 hours treatment in hyperthermic conditions (42 degrees C). Conversely, a slight potentiation of a 2 hours pretreatment with MX and DX was obtained by a 24 hours post treatment with LND.
authors.affiliation: Divisione di Oncologia Sperimentale C, Istituto Nazionale Tumori, Milano, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Villa
authors.forename: D
authors.initials: D
authors.lastname: Gornati
authors.forename: N
authors.initials: N
authors.lastname: Zaffaroni
authors.forename: S
authors.initials: S
authors.lastname: Veneroni
authors.forename: P
authors.initials: P
authors.lastname: Bidoli
authors.forename: A
authors.initials: A
authors.lastname: Severini
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D018281:Cholangiocarcinoma / Q000188:drug therapy* / Q000473:pathology; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D006979:Hyperthermia, Induced
chemical_list: D000970:Antineoplastic Agents