Analysis of human herpesvirus type 8 infection in AIDS-related and AIDS-unrelated primary central nervous system lymphoma.
Human herpesvirus type 8 (HHV-8) has been proposed as a pathogenetic factor for immunosuppression-associated primary central nervous system lymphoma (PCNSL). To verify this hypothesis, HHV-8 infection was investigated in 31 persons with PCNSL (16 AIDS-related, 15 AIDS-unrelated) and in 30 persons with systemic B cell non-Hodgkin's lymphomas (B-NHL; 15 AIDS-related, 15 AIDS-unrelated). All subjects with PCNSL scored negative by single-step polymerase chain reaction (PCR), suggesting a tumor virus load of <100 viral copies/200,000 human haploid genome equivalents (HHGE). By applying Poisson assumptions to nested PCR, 16 of 31 persons with PCNSL were devoid of HHV-8 sequences: 1 subject with AIDS and PCNSL had 1-100 viral copies/200,000 HHGE, and 14 with PCNSL had <1 viral copy/200,000 HHGE. Similarly, 10 of 30 persons with systemic B-NHL were devoid of HHV-8 sequences; 20 had <1 viral copy/200,000 HHGE. The extremely low levels of infection rule out a role of HHV-8 in PCNSL pathogenesis and are consistent with HHV-8 infection of bystander cells contaminating the tumor clone.
Gaidano
G
G
Dipartimento di Scienze Biomediche e Oncologia Umana, Università di Torino, Turin, Italy.
Capello
D
D
Pastore
C
C
Antinori
A
A
Gloghini
A
A
Carbone
A
A
Larocca
L M
LM
Saglio
G
G
D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D001483:Base Sequence; D016543:Central Nervous System Neoplasms / Q000150:complications* / Q000473:pathology / Q000821:virology; D017931:DNA Primers; D004279:DNA, Viral / Q000737:chemistry; D006566:Herpesviridae Infections / Q000150:complications* / Q000821:virology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000150:complications* / Q000473:pathology / Q000821:virology; D016393:Lymphoma, B-Cell / Q000150:complications* / Q000473:pathology / Q000821:virology; D016012:Poisson Distribution; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004279:DNA, Viral