Developmental analysis of factors binding to the mouse 68-kDa neurofilament promoter.
Whole tissue extracts prepared from mouse brain regions at various postnatal ages were characterized for binding of factors to the DNase I hypersensitive site (HSSI) which is located closest to the transcription start site of the 68-kDa mouse neurofilament gene (NF-L). Gel mobility shift assays detected changes in factor binding during postnatal development of the neocortex. Competition experiments suggested that one of the complexes resulted from factor binding to a 9 bp sequence found in both the light and medium neurofilament promoter regions (NF-L/M). Gel mobility shifts performed with an oligonucleotide probe containing the NF-L/M sequence detected two brain-specific DNA-protein complexes, and a third complex in both brain and liver. During cerebellar and neocortical development, one of the NF-L/M complexes was most intense at postnatal day 10 when transcription of the NF-L gene is upregulated.
Kure
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Department of Zoology, University of Toronto, West Hill Ontario, Canada.
Brown
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IR
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D008099:Liver / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D016900:Neurofilament Proteins / Q000096:biosynthesis* / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D016900:Neurofilament Proteins; C044882:neurofilament protein L