Collagenolytic activity and its sensitivity to doxycycline inhibition in tracheal aspirates of horses with chronic obstructive pulmonary disease.
The collagenolytic activity and its sensitivity to doxycycline inhibition in tracheal aspirates (TA) of horses with chronic obstructive pulmonary disease (COPD) was analyzed with SDS-PA gel electrophoresis (SDS-PAGE), using Type 1 collagen as the substrate. Both autoactive and total collagenase activities were significantly higher in TAs of horses with symptomatic COPD than in TAs of healthy horses. Doxycycline inhibition studies suggest that most of the TA collagenase is of the neutrophil type (MMP-8), but some is derived from other cells such as fibroblasts and monocyte/macrophages (MMP-1) and bacteria (bacterial collagenases). Drugs inhibiting collagenases in the respiratory tract might be worth a trial in the treatment of COPD in horses.
Koivunen
A L
AL
Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Finland.
Maisi
P
P
Konttinen
Y T
YT
Prikk
K
K
Sandholm
M
M
D000818:Animals; D003094:Collagen / Q000378:metabolism*; D017364:Collagenases / Q000302:isolation & purification / Q000378:metabolism*; D004318:Doxycycline / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006734:Horse Diseases; D006736:Horses; D008173:Lung Diseases, Obstructive / Q000201:enzymology / Q000662:veterinary*; D061965:Matrix Metalloproteinase Inhibitors; D009092:Mucous Membrane / Q000201:enzymology; D013396:Suction; D014132:Trachea
D061965:Matrix Metalloproteinase Inhibitors; D003094:Collagen; D017364:Collagenases; D004318:Doxycycline