title: Discordant expression of major histocompatibility complex class II antigens and invariant chain in interstitial dendritic cells. Implications for self-tolerance and immunity.
abstract: BACKGROUND
  The invariant chain plays a crucial role in antigen presentation by influencing the expression and peptide loading of major histocompatibility complex (MHC) class II molecules. Therefore, coordinate expression of these molecules is important for antigen presentation.
  
  
  METHODS
  Immunohistological studies were performed on frozen sections of many rat tissues in order to examine expression of invariant chain and MHC class II antigens.
  
  
  RESULTS
  Although coordinately regulated in most tissues, the interstitial dendritic cell (and the renal tubular epithelial cell) was always negative for invariant chain, while strongly positive for MHC class II antigens. However, renal tubular epithelial cells strongly expressed invariant chain during kidney graft rejection.
  
  
  CONCLUSIONS
  The absence of invariant chain in interstitial dendritic cells is unexpected, in view of their presumed function as sentinel antigen-presenting cells in the connective tissues. This might have important implications for antigen presentation for tolerance and immunity.
authors.affiliation: Division of Cell and Molecular Biology, Institute of Child Health, University College London Medical School, England.
authors.forename: M
authors.initials: M
authors.lastname: Saleem
authors.forename: G J
authors.initials: GJ
authors.lastname: Sawyer
authors.forename: R A
authors.initials: RA
authors.lastname: Schofield
authors.forename: N D
authors.initials: ND
authors.lastname: Seymour
authors.forename: K
authors.initials: K
authors.lastname: Gustafsson
authors.forename: J W
authors.initials: JW
authors.lastname: Fabre
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D007108:Immune Tolerance; D007109:Immunity; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology / Q000473:pathology; D007684:Kidney Tubules / Q000737:chemistry / Q000166:cytology / Q000276:immunology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000737:chemistry / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; C045084:invariant chain