Inappropriately high iron regulatory protein activity in monocytes of patients with genetic hemochromatosis.
In genetic hemochromatosis (GH), excess iron is deposited in parenchymal cells, whereas little iron is found in reticuloendothelial (RE) cells until the later stages of the disease. As iron absorption is inversely related to RE cells stores, a failure of RE to retain iron has been proposed as the basic defect in GH. In RE cells of GH subjects, we examined the activity of iron regulatory protein (IRP), a reliable indicator of the elusive regulatory labile iron pool, which modulates cellular iron homeostasis through control of ferritin (Ft) and transferrin receptor gene expression. RNA-bandshift assays showed a significant increase in IRP activity in monocytes from 16 patients with untreated GH compared with 28 control subjects (1.5-fold) and five patients with secondary hemochromatosis (SH) with similar iron burden (fourfold). In 17 phlebotomy-treated GH patients, IRP activity did not differ from that of control subjects. In both GH and SH monocyte-macrophages, Ft content increased by twofold and the L subunit-rich isoferritin profile was unchanged as compared with controls. IRP activity was still upregulated in vitro in monocyte-derived macrophages of GH subjects but, following manipulations of iron levels, was modulated normally. Therefore, the sustained activity of monocyte IRP found in vivo in monocytes of GH patients is not due to an inherent defect of its control, but is rather the expression of a critical abnormality of iron metabolism, eg, a paradoxical contraction of the regulatory iron pool. By preventing Ft mRNA translation, high IRP activity in monocytes may represent a molecular mechanism contributing to the inadequate Ft accumulation and insufficient RE iron storage in GH.
Cairo
G
G
Centro di Studio sulla Patologia Cellulare CNR, Università degli Studi, Milano, Italy.
Recalcati
S
S
Montosi
G
G
Castrusini
E
E
Conte
D
D
Pietrangelo
A
A
D000328:Adult; D000368:Aged; D015415:Biomarkers; D005260:Female; D005293:Ferritins / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation; D006432:Hemochromatosis / Q000097:blood / Q000235:genetics* / Q000628:therapy; D006706:Homeostasis; D006801:Humans; D007501:Iron / Q000097:blood*; D035925:Iron-Regulatory Proteins; D007506:Iron-Sulfur Proteins / Q000032:analysis*; D008264:Macrophages / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry*; D018962:Phlebotomy; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000032:analysis*
D015415:Biomarkers; D035925:Iron-Regulatory Proteins; D007506:Iron-Sulfur Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D005293:Ferritins; D007501:Iron