Identification of key gene products required for acquisition of plasmin-like enzymatic activity by group A streptococci.
Group A streptococci incubated in human plasma can acquire a plasmin-like enzymatic activity. This process involves at least two bacterial proteins and two human protein cofactors. In this study, the key bacterial proteins were identified by using a series of isogenic mutants of group A isolate, CS101. These studies confirm a key role for the secreted plasminogen activator, streptokinase, and identify the major surface fibrinogen-binding protein as the product of the mrp gene. The requirement for human fibrinogen and plasminogen as key cofactors was also confirmed.
Christner
R
R
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008, USA.
Li
Z
Z
Raeder
R
R
Podbielski
A
A
Boyle
M D
MD
D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D005340:Fibrinogen / Q000378:metabolism*; D005341:Fibrinolysin; D005798:Genes, Bacterial; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D010958:Plasminogen / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D013300:Streptokinase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D008565:Membrane Proteins; C105863:Mrp22 protein, Streptococcus; D011994:Recombinant Proteins; D005340:Fibrinogen; D010958:Plasminogen; D013300:Streptokinase; D005341:Fibrinolysin