Cytophotometric determination of heterochromatin base composition in interphase nuclei of plant cells.
The use of DNA base-specific fluorochromes and static cytophotometry at different thresholds of fluorescence intensity is proposed for studying the base composition of differently condensed chromatin fractions in plant interphase nuclei. Results are reported of such analyses on nuclei from leaflets of Posidonia oceanica and Helianthus annuus, which confirm other findings obtained on the same materials by biochemical and molecular methods.
Cavallini
A
A
Dipartimento di Biologia delle Piante Agrarie, Università di Pisa, Italy.
Cionini
G
G
Polizzi
E
E
Natali
L
L
D001482:Base Composition; D002467:Cell Nucleus / Q000737:chemistry; D014128:Chromomycin A3; D003592:Cytophotometry; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes; D006570:Heterochromatin / Q000737:chemistry*; D007211:Indoles; D007399:Interphase; D010944:Plants / Q000737:chemistry*; D011419:Propidium
D005456:Fluorescent Dyes; D006570:Heterochromatin; D007211:Indoles; D011419:Propidium; C007293:DAPI; D004247:DNA; D014128:Chromomycin A3