Production of IL-5 and granulocyte-macrophage colony-stimulating factor by naive human mast cells activated by high-affinity IgE receptor ligation.
BACKGROUND
The late-phase allergic reaction is an eosinophilic inflammatory response that begins several hours after allergen exposure, may persist for 24 hours, and is an important pathogenic mechanism in allergic disease.


OBJECTIVE
Cultured naive human mast cells were used to investigate whether mast cells are a direct source of the eosinophil-promoting cytokines IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor (GM-CSF).


METHODS
Naive human mast cells were derived from bone marrow mononuclear cells cultured in the presence of stem-cell factor. Cytokine message and protein production in response to high-affinity IgE receptor ligation of cultured mast cells were measured by semiquantitative polymerase chain reaction and ELISA, respectively.


RESULTS
IL-5, IL-3, and GM-CSF messenger RNA increased within 2 hours of mast cell activation, with IL-5 and GM-CSF message remaining elevated for 24 hours, whereas IL-3 mRNA rapidly declined. IL-5 and GM-CSF protein were measurable 4 to 6 hours after stimulation and peaked by 24 and 12 hours, respectively. IL-3 protein was not detectable.


CONCLUSIONS
These findings demonstrate that naive mast cells do not constitutively produce IL-5 or GM-CSF protein but are a major source of these eosinophilotropic cytokines on high-affinity IgE receptor ligation.
Bressler
R B
RB
Department of Medicine, Baylor College of Medicine, Houston, TX, USA.
Lesko
J
J
Jones
M L
ML
Wasserman
M
M
Dickason
R R
RR
Huston
M M
MM
Cook
S W
SW
Huston
D P
DP
D001483:Base Sequence; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis; D003592:Cytophotometry; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis*; D006636:Histamine Release; D006801:Humans; D007150:Immunohistochemistry; D007377:Interleukin-3 / Q000032:analysis / Q000096:biosynthesis; D015848:Interleukin-5 / Q000032:analysis / Q000096:biosynthesis*; D008024:Ligands; D008407:Mast Cells / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017455:Receptors, IgE / Q000378:metabolism*; D015854:Up-Regulation
D016207:Cytokines; D007377:Interleukin-3; D015848:Interleukin-5; D008024:Ligands; D017455:Receptors, IgE; D016178:Granulocyte-Macrophage Colony-Stimulating Factor