title: Platelet-endothelial cell adhesion molecule-1 (PECAM-1/CD31) tyrosine phosphorylation state changes during vasculogenesis in the murine conceptus.
abstract: Vasculogenesis, the differentiation of mesodermal cells to angioblasts and the subsequent formation of blood islands and blood vessels by angioblasts in the conceptus, is a dynamic process modulated, in part, by cell-extracellular matrix and cell-cell interactions in the presence of a variety of growth factors and morphogens. In this report we demonstrate differential tyrosine phosphorylation of platelet-endothelial cell adhesion molecule-1 (PECAM-1) during the formation of blood islands and vessels from clusters of extraembryonic and embryonic angioblasts in the murine conceptus. In addition, we identify the phosphorylation of a particular tyrosine residue in the PECAM-1 cytoplasmic domain, Tyr686, which has the potential of mediating binding to Src homology 2 domain-containing proteins, affecting PECAM-1 cellular localization and endothelial cell migration.
authors.affiliation: Department of Pathology, Yale University School of Medicine, New Haven, CT 06520-8023, USA.
authors.forename: E
authors.initials: E
authors.lastname: Pinter
authors.forename: M
authors.initials: M
authors.lastname: Barreuther
authors.forename: T
authors.initials: T
authors.lastname: Lu
authors.forename: B A
authors.initials: BA
authors.lastname: Imhof
authors.forename: J A
authors.initials: JA
authors.lastname: Madri
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D004622:Embryo, Mammalian / Q000098:blood supply* / Q000737:chemistry / Q000378:metabolism*; D007106:Immune Sera / Q000737:chemistry; D051379:Mice; D018919:Neovascularization, Physiologic; D010766:Phosphorylation; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D011233:Precipitin Tests; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D007106:Immune Sera; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014443:Tyrosine