Application of suppression PCR to the megaprimer method for site-directed mutagenesis.
Combination of suppression polymerase chain reaction (PCR) and megaprimer method of site-directed mutagenesis allows specific reamplification of extended megaprimer. This modification is proposed for templates that are difficult to amplify when standard megaprimer procedures do not generate sufficient yield of mutated product.
Kaluz
S
S
Department of Physiology and Environmental Science, University of Nottingham, Loughborough, UK.sbzsk@sbn3.nott.AC.UK
Kaluzova
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M
Flint
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AP
D000818:Animals; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D018045:Receptors, Oxytocin / Q000235:genetics; D012756:Sheep
D017931:DNA Primers; D018076:DNA, Complementary; D018045:Receptors, Oxytocin