Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2.
Inherited mutations in the human BRCA2 gene cause about half of the cases of early-onset breast cancer. The embryonic expression pattern of the mouse Brca2 gene is now defined and an interaction identified of the Brca2 protein with the DNA-repair protein Rad51. Developmental arrest in Brca2-deficient embryos, their radiation sensitivity, and the association of Brca2 with Rad51 indicate that Brca2 may be an essential cofactor in the Rad51-dependent DNA repair of double-strand breaks, thereby explaining the tumour-suppressor function of Brca2.
Sharan
S K
SK
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA.
Morimatsu
M
M
Albrecht
U
U
Lim
D S
DS
Regel
E
E
Dinh
C
C
Sands
A
A
Eichele
G
G
Hasty
P
P
Bradley
A
A
D016475:3T3 Cells; D000818:Animals; D024682:BRCA2 Protein; D001755:Blastocyst / Q000528:radiation effects; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology*; D004622:Embryo, Mammalian / Q000166:cytology; D005314:Embryonic and Fetal Development / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays; D015870:Gene Expression; D018390:Gene Targeting; D016147:Genes, Tumor Suppressor; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009363:Neoplasm Proteins / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D051135:Rad51 Recombinase; D011836:Radiation Tolerance / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D013234:Stem Cells; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D024682:BRCA2 Protein; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase; C495154:Rad51 protein, mouse