Detection of an antigenic protein of Leptospira interrogans which shares epitopes with the equine cornea and lens.
A protein epitope which is involved in an antigenic relationship between equine ocular tissues and Leptospira interrogans was detected in homogenates of the bacterium. The antigenic determinant was harboured on a peptide structure which was shown to be sensitive to the action of denaturing and reducing agents by means of Western blotting. The outer surface of the leptospires appeared to be free of this epitope as was proved by dot-blot and electron microscopic studies.
Parma
A E
AE
Department of Animal Health, Immunochemistry, Faculty of Veterinary Science, University of the Centre, Tandil, Argentina.
Sanz
M E
ME
Lucchesi
P M
PM
Mazzonelli
J
J
Petruccelli
M A
MA
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D003315:Cornea / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D000939:Epitopes / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000276:immunology*; D007921:Leptospira interrogans / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000662:veterinary; D011817:Rabbits
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes