title: Implication of neutral polysaccharides associated to alginate in inhibition of murine macrophage response to Pseudomonas aeruginosa.
abstract: There is evidence that exopolysaccharides (EPS) contribute to the persistence of Pseudomonas aeruginosa in cystic fibrosis lung. However, the relationship between the chemical composition of EPS and the modulation of phagocytic cells is poorly understood. In order to evaluate the role of the chemical composition of EPS in macrophage behavior changes, we pretreated macrophages with characterized EPS and assessed P. aeruginosa phagocytosis and reactive oxygen intermediate (ROI) production. The results showed that alginate and neutral polysaccharides are involved in phagocytic impairment of P. aeruginosa. Moreover, alginates were able to prime macrophages for increased P. aeruginosa-induced macrophage oxidative burst as determined by chemiluminescence. In contrast, neutral polysaccharides are responsible for the decrease of ROI by a scavenging effect evaluated by the xanthine-xanthine oxidase system. This study showed that the content of P. aeruginosa EPS in alginate, but also in neutral polysaccharides, influences the behavior of strains towards phagocytosis and macrophage oxidative burst.
authors.affiliation: Laboratorie de Bactériologie-Virologie-Hygiène, Hôpital Rangueil, Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Pasquier
authors.forename: N
authors.initials: N
authors.lastname: Marty
authors.forename: J L
authors.initials: JL
authors.lastname: Dournes
authors.forename: G
authors.initials: G
authors.lastname: Chabanon
authors.forename: B
authors.initials: B
authors.lastname: Pipy
mesh_terms: D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D005260:Female; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008163:Luminescent Measurements; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000502:physiology*; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D011135:Polysaccharides, Bacterial; D017382:Reactive Oxygen Species; D020723:Glucuronic Acid; D014969:Xanthine Oxidase