C-myc overexpression facilitates radiation-induced DHFR gene amplification.
To investigate the role of myc-overexpression on radiation-induced amplification of the dihydrofolate reductase gene (DHFR) we compared diploid Chinese hamster ovary cells (CHO-9) to cells of the same line that had been stably transfected with a dexamethasone-inducible c-myc cDNA. The application of flow-cytofluorometry and fluorescent in situ hybridization (FISH) allowed the evaluation of an increase in DHFR gene copy number following radiation treatment without the use of a preceding selection procedure. We show that DHFR gene amplification may occur independently of p53 status in cells overexpressing c-myc.
Lücke-Huhle
C
C
Research Center Karlsruhe, Institute of Genetics, Germany.
Mai
S
S
Moll
J
J
D000818:Animals; D015139:Blotting, Southern; D016466:CHO Cells; D006224:Cricetinae; D005434:Flow Cytometry; D005784:Gene Amplification / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D016259:Genes, myc; D017404:In Situ Hybridization, Fluorescence; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D013997:Time Factors; D014162:Transfection
D013762:Tetrahydrofolate Dehydrogenase