Cloning and sequence analysis of a cDNA encoding a crustacean hyperglycemic hormone from the Kuruma prawn Penaeus japonicus.
The crustacean hyperglycemic hormones (CHHs) are released from the X-organ sinus gland complex and regulate the glucose level in the hemolymph. In the present study, we have isolated a complementary DNA that encodes a CHH (Pej-SGP-III) of the Kuruma prawn Penaeus japonicus to study its expression and the structure of its precursor. In a cDNA clone of 774 base pairs (bp), an open reading frame of 345 bp was found. The conceptually translated protein consists of a signal peptide (24 residues), a CHH-precursor-related peptide (CPRP, 17 residues), and Pej-SGP-III (72 residues). This CPRP in P.japonicus was homologous to, but significantly shorter than, those in other crustacean species. In a Northern blot analysis, the cDNA specifically hybridized to a seemingly single mRNA species (approximately 0.8 kb) in the eyestalk. This mRNA was not detected in the hepatopancreas or abdominal muscle.
Ohira
T
T
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Watanabe
T
T
Nagasawa
H
H
Aida
K
K
D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry*; D015870:Gene Expression; D007447:Invertebrate Hormones / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism*; D011498:Protein Precursors / Q000096:biosynthesis / Q000737:chemistry; D021382:Protein Sorting Signals / Q000096:biosynthesis* / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D060829:Arthropod Proteins; D018076:DNA, Complementary; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D011498:Protein Precursors; D021382:Protein Sorting Signals; C015947:hyperglycemic hormone, crustacean