Isolation of pathogen/stress-inducible cDNAs from alfalfa by mRNA differential display.
Differential display of mRNA was used to isolate a full-length (SRG1) and a partial (SRG2) alfalfa cDNA induced during infection with the fungal pathogen Colletotrichum trifolii. The deduced amino acid sequences are similar to each other and resemble plant defense-related proteins and tree pollen allergens. SRG1 is a member of a gene family in alfalfa, which may also include the putative defense-related gene PR10. Unlike many defense-related genes described in similar systems, expression of SRG1-like genes does not correlate with resistance to C. trifolii. We speculate SRG1 is induced in response to plant stress.
Truesdell
G M
GM
School of Biological Sciences, University of Nebraska-Lincoln, 68583-0722, USA.
Dickman
M B
MB
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics*; D000455:Medicago sativa / Q000235:genetics*; D003904:Mitosporic Fungi / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant