title: Role of Drosophila alpha-adaptin in presynaptic vesicle recycling.
abstract: Rapid flow of information in the nervous system involves presynaptic vesicle recycling by clathrin-mediated endocytosis, an event triggered by the alpha-adaptin-containing AP2 complex. We identified a Drosophila alpha-adaptin expressed in the garland cells, imaginal discs, and the CNS. Here we show its role in presynaptic vesicle recycling. In presynaptic terminals, alpha-adaptin defines a network-like membrane structure to which the GTPase dynamin is recruited. alpha-adaptin is necessary for the formation of clathrin-coated pits and participates in the dynamin-dependent release of coated vesicles from the membrane surface. Our results suggest an alpha-adaptin-dependent control of the vesicle cycle that maintains the balance between the amount of vesicle- and surface-associated membranes.
authors.affiliation: Max-Planck-Institut für biophysikalische Chemie, Abt. Molekulare Entwicklungsbiologie, Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: González-Gaitán
authors.forename: H
authors.initials: H
authors.lastname: Jäckle
mesh_terms: D033962:Adaptor Protein Complex 2; D033965:Adaptor Protein Complex alpha Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D000918:Antibody Specificity; D004330:Drosophila; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D017344:Genes, Insect / Q000502:physiology; D017403:In Situ Hybridization; D007814:Larva / Q000737:chemistry / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008854:Microscopy, Electron; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D017729:Presynaptic Terminals / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D013572:Synaptic Vesicles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D033962:Adaptor Protein Complex 2; D033965:Adaptor Protein Complex alpha Subunits; D033942:Adaptor Proteins, Vesicular Transport; D008565:Membrane Proteins; D012333:RNA, Messenger; D020558:GTP Phosphohydrolases; D034281:Dynamins