The effect of abomasal infusion of casein and recombinant somatotropin hormone injection on nitrogen balance and amino acid fluxes in portal-drained viscera and net hepatic and total splanchnic blood in Holstein steers.
Six Holstein steers, 6 mo of age, with a BW range of 180 to 200 kg were used to determine effects of abomasal casein infusion and recombinant bovine somatotropin on amino acid absorption and metabolism in the gut and liver. Catheters were positioned in the hepatic vein, the hepatic portal vein, two mesenteric veins, and a mesenteric artery. Using the same basal diet, treatments consisted of 1) basal diet only (Control), 2) abomasal casein infusion of 300 g/d (Casein), and 3) abomasal casein infusion plus daily injection of 20 mg of bovine somatotropin (ST). All steers were fed the basal diet at a rate of 24 g/kg of BW on a DM basis, in 12 equal meals at 2-h intervals. Initially, Casein and ST treatments were in a balanced cross-over design, and then all steers received the Control treatment. Casein infusion tended to increase BW gain but did not affect BW gain per unit of N intake. The ST treatment increased weight gain (P < .01) and efficiency of dietary DM (P < .01) and N (P < .01) utilization by more than 40% and increased the percentage of absorbed N retained. Nonessential, essential, and total amino acid fluxes in portal-drained viscera (PDV) and their net removal by the liver were not affected by casein infusion. However, ST tended to reduce PDV flux of essential, nonessential, and total amino acids by approximately 30%, and their removal by the liver (P < .08). As a result, ST increased, compared with casein, release of essential (P < .06) and total (P < .10) amino acids from the total splanchnic pool (TSP) into peripheral blood. The ST treatment increased the availability of absorbed amino acids to peripheral tissues, but it did not affect the amino acid profile delivered from splanchnic tissues, especially that of essential amino acids.
Bruckental
I
I
Department of Animal Sciences, University of Maryland, College Park 20742, USA.
Huntington
G B
GB
Baer
C K
CK
Erdman
R A
RA
D000018:Abomasum; D000596:Amino Acids / Q000097:blood* / Q000378:metabolism*; D000641:Ammonia / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D002364:Caseins / Q000008:administration & dosage / Q000494:pharmacology*; D002417:Cattle / Q000097:blood* / Q000378:metabolism*; D005243:Feces / Q000737:chemistry; D013006:Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D007262:Infusions, Intravenous / Q000379:methods / Q000662:veterinary; D007273:Injections, Intramuscular / Q000379:methods / Q000662:veterinary; D008099:Liver / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D009584:Nitrogen / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D010100:Oxygen / Q000097:blood; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013152:Splanchnic Circulation; D014781:Viscera / Q000378:metabolism*
D000596:Amino Acids; D001786:Blood Glucose; D002364:Caseins; D011994:Recombinant Proteins; D000641:Ammonia; D013006:Growth Hormone; D009584:Nitrogen; D010100:Oxygen