TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family.
We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240 and p230 were copurified with telomerase activity in a series of extensive purification experiments. These results strongly suggest that the TLP1 proteins are components of, or are closely associated with, the rat telomerase. A pulse-chase experiment showed that p240 is modified to p230 in vivo. p230 was the dominant form in telomerase-positive cells, suggesting that modification of the TLP1 protein may regulate telomerase activity in vivo.
Nakayama
J
J
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
Saito
M
M
Nakamura
H
H
Matsuura
A
A
Ishikawa
F
F
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D019556:COS Cells / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006801:Humans; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D019098:Telomerase / Q000235:genetics*
D018076:DNA, Complementary; D019098:Telomerase