Effects of ADP, DTT, and Mg2+ on the ion-conductive property of chloroplast H+-ATPase(CF0-CF1) reconstituted into bilayer membrane.
The purified CF0-CF1 complex of spinach was incorporated into planar lipid bilayer membranes (LBMs) formed with soybean phospholipid, and the transmembrane ion-transmission properties were studied under voltage-clamp mode. The results showed that the presence of both ADP and Pi decreased the membrane current while Dithiothreitol could evoke a stronger conductive change of CF0-CF1 containing LBMs when Ca2+ or Mg2+ exists. Mg2+ can dramatically increase the CF0-CF1 conductance in various conditions. These results indicated that the H(+)-transitive function of CF0-CF1 reconstituted in bilayer is sensitive to those factors which can affect its ATP synthase activity in vivo.
Ma
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XH
Shanghai Institute of Physiology, Academia Sinica, People's Republic of China.
Shi
Y L
YL
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002736:Chloroplasts / Q000201:enzymology* / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011522:Protons; D018724:Spinacia oleracea
D007473:Ion Channels; D008051:Lipid Bilayers; D011522:Protons; D000244:Adenosine Diphosphate; D006180:Proton-Translocating ATPases; D008274:Magnesium; D002118:Calcium; D004229:Dithiothreitol