title: Seromuscular gastrocystoplasty in rats.
abstract: Long-term results of seromuscular gastrocystoplasty have not been reported in the literature. In the presented study, a seromuscular gastrocystoplasty technique in rats and related biochemical, physiological, and histopathologic results are discussed. Fifty rats were grouped as follows: control and sham (group A), animals with gastrocystoplasty (group B), and animals with seromuscular gastrocystoplasty (group C). Urine pH, blood pH, and bicarbonate levels, bladder capacity, and end filling pressures were evaluated in all groups. After gastrocystoplasty and seromuscular gastrocystoplasty both group B and C showed a statistically significant increase in bladder capacity. Aciduria developed in 22% of rats in group B. In group A and C aciduria was not noted. No statistically significant difference was observed in blood pH, bicarbonate levels, and end-filling pressure measurements in each group of rats. Significant histopathologic changes of bladder occurred in the gastrocystoplasty group. In the 13 bladders examined histologically in group B, 2 had a papilloma on the mucosal surface of the transplanted gastric patch. The bladder mucosa adjacent to the transplanted gastric patch had pronounced hyperplastic and squamous metaplastic changes. In the 15 bladders examined histologically in group C none had a papilloma on the gastric segments. Squamous metaplasia in the bladder mucosa adjacent to the transplanted gastric patch was noted only in 2 rats of this group. Two rats also had bladder calculi in the lumen. Possible etiologies and differences of gastrocystoplasty and seromuscular gastrocystoplasty related pathological changes between two groups are discussed.
authors.affiliation: Clinic of Pediatric Surgery, Social Security Council Ankara Children's Hospital, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Demirbilek
authors.forename: H F
authors.initials: HF
authors.lastname: Atayurt
authors.forename: U
authors.initials: U
authors.lastname: Han
authors.forename: G
authors.initials: G
authors.lastname: Aydin
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000009:adverse effects; D006863:Hydrogen-Ion Concentration; D008297:Male; D009092:Mucous Membrane / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000637:transplantation*; D014182:Transplantation, Autologous / Q000009:adverse effects; D001743:Urinary Bladder / Q000473:pathology / Q000502:physiology / Q000601:surgery*; D001744:Urinary Bladder Calculi / Q000209:etiology; D014556:Urine; D014563:Urodynamics
chemical_list: D001639:Bicarbonates