Effect of ferrous ion on amino acid metabolism in mildiomycin production by Streptoverticillium rimofaciens.
The physiological features of the mildiomycin production by Streptoverticillium rimofaciens were examined in iron-sufficient and -deficient media. Activities of NADP-linked glutamate dehydrogenase (GDH) and aspartate aminotransferase (AAT) were markedly enhanced by the addition of 10 micrograms/ml of ferrous ion into culture. Ammonium nitrogen assimilation increased with the increase in mildiomycin production. These indicate that ferrous ion contributes the supply of amino acids as a precursor of mildiomycin production. In the iron-sufficient medium, glutamate, aspartate, serine and arginine in cells were 2 to 10-fold to those in the iron-deficient medium. The major amino acid excreted from cells was arginine in the iron-sufficient culture, while in the iron-deficient culture, valine. Change in the amino acid profile by addition of ferrous ion was useful for mildiomycin biosynthesis, in which ferrous ion played a leading role in amino acid metabolism.
Kishimoto
K
K
Technology Department, Hikari Branch Lab., Takeda Chemical Industries, Ltd., Yamaguchi Prefecture, Japan.
Park
Y S
YS
Okabe
M
M
Akiyama
S
S
D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000935:Antifungal Agents / Q000096:biosynthesis*; D001224:Aspartic Acid / Q000096:biosynthesis; D003596:Cytosine / Q000031:analogs & derivatives / Q000096:biosynthesis; D005285:Fermentation; D005296:Ferrous Compounds / Q000494:pharmacology*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005970:Glutamate Synthase / Q000378:metabolism; D013306:Streptomycetaceae / Q000378:metabolism*
D000596:Amino Acids; D000935:Antifungal Agents; D005296:Ferrous Compounds; D001224:Aspartic Acid; D003596:Cytosine; D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; C017539:mildiomycin