Structure and phase behaviour of dimyristoylphosphatidic acid/poly(L-lysine) systems.
Differential scanning calorimetry (DSC) and X-ray diffraction studies on (DMPA)/poly(L-lysine) systems are reported. DSC studies revealed that addition of poly(L-lysine) to DMPA bilayers raises the gel to liquid-crystalline phase transition of the systems, and that this effect depends on the molecular weight of the poly(L-lysine). Small-angle X-ray diffraction measurements showed that, in the liquid-crystalline phase, the lamellar spacing of a DMPA/short-poly(L-lysine) (approximately 4000 mol. wt.) system is shorter than that of a DMPA/long-poly(L-lysine) (approximately 22000 mol. wt.). In this connection wide-angle X-ray diffraction measurements indicate that the long-poly(L-lysine) adopts a beta-sheet conformation on the DMPA bilayers in both the gel and the liquid-crystalline phases, but the short-poly(L-lysine) adopts this conformation only on gel phase DMPA bilayers. We found that the spacings of the hydrocarbon chain packing in a DMPA bilayer in the gel phase increases with temperature, while the spacing between neighbouring polypeptide chains in long-poly(L-lysine) in the beta-sheet conformation remains almost constant. These observations indicate that the positively charged lysine residues are structurally independent of the negatively charged head groups of the phospholipid. On the basis of the present results we propose a model to explain the elementary behaviour of extrinsic membrane proteins in biomembranes.
Takahashi
H
H
Department of Applied Physics, School of Engineering, Nagoya University, Chikusa-ku, Japan.
Yasue
T
T
Ohki
K
K
Hatta
I
I
D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004347:Drug Interactions; D020404:Glycerophospholipids; D006361:Heating; D008051:Lipid Bilayers / Q000737:chemistry; D008968:Molecular Conformation; D010712:Phosphatidic Acids / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
D020404:Glycerophospholipids; D008051:Lipid Bilayers; D010712:Phosphatidic Acids; D011107:Polylysine; C025602:dimyristoylphosphatidic acid