Allogeneic transplantation with CD34+-selected cells.
We report the outcome of eight patients with different hematological malignancies who were transplanted with allogeneic CD34-selected mononuclear cells following myeloablative therapy. Four patients received G-CSF mobilized CD34-enriched peripheral blood progenitor cells (PBPC) together with CD34-enriched bone marrow (BM), two patients were transplanted with allogeneic G-CSF mobilized CD34-enriched PBPC alone, and two patients received only allogeneic CD34-enriched BM cells. On average, patients received 2.66 x 10(6) CD34+-cells/kg BW (range: 0.53-8.40 x 10(6) CD34+-cells/kg body weight) and 0.57 x 10(6) CD3+-cells/kg BW (range: 0.20-1.10 x 10(6) CD3+-cells/kg BW), respectively. Seven of the eight patients engrafted (ANC > 0.5 x 10(9)/L median: day +19 (range: 16-23 days); platelets > 20 x 10(9)/L median: day +34 (range: 21-47 days); one patient died on day +16 after transplantation and was not evaluable for engraftment. Three of seven patients evaluable for acute graft-versus-host disease (GvHD) developed acute GvHD grade II which resolved upon steroid treatment. Five of the eight patients are still alive and in remission with a median follow-up of 215 days (range: 80-420 days). Causes of death included fungal infection, cerebral bleeding and sepsis. These preliminary data suggest that CD34-enriched cells can be successfully given during for allogenic transplantation following myeloablative therapy in hematological malignancies. The impact of T-cell depletion by enrichment for CD34+-cells in an attempt to reducing the incidence and/or severity of acute and/or chronic GvHD still remains to be determined.
Stockschläder
M
M
Bone Marrow Transplantation Unit, University Hospital Eppendorf, Hamburg, Germany.
Hassan
H T
HT
Zeller
W
W
Krüger
W
W
Clausen
J
J
Löliger
C
C
Dieck
A T
AT
Kröger
N
N
Link
H
H
Kabisch
H
H
Hossfeld
D K
DK
Zander
A
A
D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000379:methods*; D002469:Cell Separation; D002648:Child; D006801:Humans; D008875:Middle Aged; D010954:Plasmacytoma / Q000628:therapy; D014184:Transplantation, Homologous
D018952:Antigens, CD34