Induction of differentiation by wild-type p53 gene in a human glioma cell line.
Wild-type human p53 gene was transfected into the human glioma cell line T-98G. Transfectants were then isolated and characterized for growth potential and differentiation phenotype. Growth suppression, overexpression of GFAP, and accumulation in G1 phase were more commonly observed in transfectants than in T-98G cells. p21WAF1/CIP1 was overexpressed in transfectants, and the binding of PCNA and CDK 2 to p21WAF1/CIP1 were increased in transfectants. These results suggested the roles of p21WAF1/CIP1, PCNA, and CDK2 in regulation of differentiation in glioma cells and the gene transfer of wild-type p53 may be effective for the control of glial differentiation in glioma cells.
Kokunai
T
T
Department of Neurosurgery, Kobe University School of Medicine, Japan.
Kawamura
A
A
Tamaki
N
N
D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D042846:CDC2-CDC28 Kinases; D002455:Cell Division / Q000235:genetics; D051357:Cyclin-Dependent Kinase 2; D050759:Cyclin-Dependent Kinase Inhibitor p21; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis; D016213:Cyclins / Q000096:biosynthesis; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000235:genetics*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism; D014410:Tumor Stem Cell Assay
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D005904:Glial Fibrillary Acidic Protein; D018809:Proliferating Cell Nuclear Antigen; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases