Expression of membrane type 1 matrix metalloproteinase in human articular cartilage.
OBJECTIVE
To detect the message for membrane type 1 (MT1) matrix metalloproteinase (MMP) in articular cartilage and chondrosarcoma cells, to study its expression in osteoarthritis (OA), and to determine whether interleukin-1beta (IL-1beta) influences its expression.


METHODS
Reverse transcription-polymerase chain reaction methods were used to detect message. Cloning and sequencing were applied to confirm the sequence. Northern blotting was used to quantify the message for MT1-MMP and to compare its expression in OA cartilage with or without IL-1beta treatment. In situ hybridization was utilized to show MT1-MMP transcripts in cartilage and to study the influence of IL-1beta.


RESULTS
The results show that MT1-MMP messenger RNA (mRNA) is expressed in chondrosarcoma cells and OA chondrocytes. Results of the in situ hybridization confirmed the expression in OA cartilage as well as in normal cartilage. The level of mRNA was not modulated following IL-1beta stimulation.


CONCLUSIONS
This study shows that MT1-MMP is expressed by chondrocytes. The similarities in mRNA levels in OA and normal chondrocytes suggest that regulation of MT1-MMP mRNA may not be a primary factor in OA.
Büttner
F H
FH
Rush Medical College at Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois, USA.
Chubinskaya
S
S
Margerie
D
D
Huch
K
K
Flechtenmacher
J
J
Cole
A A
AA
Kuettner
K E
KE
Bartnik
E
E
D015152:Blotting, Northern; D001859:Bone Neoplasms / Q000201:enzymology; D002358:Cartilage, Articular / Q000187:drug effects / Q000201:enzymology*; D002813:Chondrosarcoma / Q000201:enzymology; D006801:Humans; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000494:pharmacology; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics; D010003:Osteoarthritis / Q000201:enzymology; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D012333:RNA, Messenger; D012313:RNA; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases