title: Conditioned media obtained from a human mastocytosis cell strain induce mast cells expressing chymase but not tryptase from human progenitors.
abstract: Human mast cells (MC) were derived from umbilical cord blood and bone marrow progenitors cultured in the presence of a conditioned medium from a human mastocytosis cell strain and recombinant human kit ligand (rhKL). KL induced MC of predominantly two immunophenotypes, MC(T) and MC(TC). In contrast, the conditioned medium induced MC subtypes MC(TC) and a third subtype, MC(C), positive for chymase but negative for tryptase. This study clearly demonstrates that a third type of MC, MC(C), can be induced in vitro from normal human progenitors.
authors.affiliation: School of Medicine, University of New South Wales and Department of Immunology, Allergy and Infectious Disease, St. George Hospital, Kogarah, Australia.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: S A
authors.initials: SA
authors.lastname: Krilis
mesh_terms: D001854:Bone Marrow Cells; D002478:Cells, Cultured; D053818:Chymases; D017077:Culture Media, Conditioned; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D006801:Humans; D008407:Mast Cells / Q000201:enzymology*; D008415:Mastocytosis / Q000503:physiopathology*; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D019089:Stem Cell Factor / Q000494:pharmacology; D053802:Tryptases
chemical_list: D017077:Culture Media, Conditioned; D019089:Stem Cell Factor; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases