Indium-labeled white blood cells apheresed from donors receiving G-CSF localize to sites of inflammation when infused into allogeneic bone marrow transplant recipients.
G-CSF administration to normal donors results in granulocyte apheresis yields generally greater than those observed with other neutrophil mobilizing agents. In vitro, neutrophils cultured with G-CSF exhibit prolonged survival; however, the random migration of neutrophils exposed to this agent is inhibited. Although transfused neutrophils mobilized with agents other than G-CSF migrate to sites of inflammation or infection in vivo, this has yet to be demonstrated with infusion of G-CSF-mobilized neutrophils into neutropenic human subjects. Five neutropenic allogeneic bone marrow transplant (BMT) patients each received a fresh infusion of G-CSF-mobilized indium-labeled irradiated white blood cells (WBC) apheresed from HLA-matched normal donors on day +5 post-transplant. Localization of activity on delayed scintigraphic images of indium-labeled WBC scans to sites of tissue damage (oral/nasopharynx in two patients with mucositis and terminal ileum/cecum in one with diarrhea) occurred, and supports the hypothesis that G-CSF-mobilized HLA-matched donor neutrophils which have been irradiated are functional after infusion into neutropenic recipients.
Adkins
D
D
Washington University School of Medicine, Department of Internal Medicine, St Louis, MO 63110-1093, USA.
Goodgold
H
H
Hendershott
L
L
Johnston
M
M
Cravens
D
D
Spitzer
G
G
D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D002465:Cell Movement; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D006801:Humans; D007205:Indium Radioisotopes; D007249:Inflammation / Q000000981:diagnostic imaging* / Q000473:pathology / Q000628:therapy; D007937:Leukapheresis; D017708:Leukocyte Transfusion; D019520:Living Donors; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology / Q000637:transplantation; D011877:Radionuclide Imaging; D014184:Transplantation, Homologous
D007205:Indium Radioisotopes; D016179:Granulocyte Colony-Stimulating Factor