Tau phosphorylation in cells transfected with wild-type or an Alzheimer's disease mutant Presenilin 1.
We have studied the effect of overexpressing either wild-type or an Alzheimer's disease mutant Presenilin 1 (PS1) on tau phosphorylation in transfected Chinese hamster ovary (CHO) and COS cells. Tau transfected into these cells is predominantly non-phosphorylated at many PHF-tau sites but co-transfection with the tau kinase glycogen synthase kinase-3 beta (GSK-3 beta) induces phosphorylation that generates epitopes for several phosphorylation-dependent antibodies. Co-transfection of tau with either wild-type or mutant PS1 did not alter tau phosphorylation as detected by five different antibodies. Likewise, co-transfection of the PS1s did not influence GSK-3 beta-mediated tau phosphorylation. The implications of these results for the pathogenesis of Alzheimer's disease are discussed.
Irving
N G
NG
Department of Neuroscience, Institute of Psychiatry, Denmark Hill, London, UK.
Miller
C C
CC
D000544:Alzheimer Disease / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D016466:CHO Cells / Q000502:physiology; D019556:COS Cells / Q000502:physiology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D006224:Cricetinae; D015870:Gene Expression / Q000502:physiology; D038341:Glycogen Synthase Kinases; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D016296:Mutagenesis / Q000502:physiology; D009410:Nerve Degeneration / Q000235:genetics; D016874:Neurofibrillary Tangles / Q000201:enzymology / Q000235:genetics; D010766:Phosphorylation; D017354:Point Mutation / Q000502:physiology; D053764:Presenilin-1; D014162:Transfection; D016875:tau Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D008565:Membrane Proteins; D053764:Presenilin-1; D016875:tau Proteins; D038341:Glycogen Synthase Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases