High concentration of the EBV latent membrane protein 1 in glycosphingolipid-rich complexes from both epithelial and lymphoid cells.
Latent Membrane Protein 1 (LMP1) is an EBV-transforming protein which is detected both in lymphoblastoid cell lines-resulting from EBV-immortalization in vitro- and in undifferentiated nasopharyngeal carcinoma (NPC), an EBV-associated malignancy of epithelial origin. To better define LMP1 subcellular targets, LMP1 distribution was analyzed in cellular glycosphingolipid-rich complexes (GSL-complexes) derived from epithelial and lymphoid cells. These complexes are obtained by extraction of glycosphingolipid-rich membrane domains (GSL-domains), which are clustering sites for heterotrimeric G-proteins and G-protein-associated receptors. LMP1 concentration was enriched 50-fold in GSL-complexes extracted from a NPC tumor line, C15. High concentrations of LMP1 were also observed in GSL-complexes derived from cultured lymphoid and epithelial cells. These data suggest that association with GSL-domains is an important step in LMP1 trafficking and is probably required for some aspects of its biological activity.
Clausse
B
B
Laboratoire de Biologie des Tumeurs Humaines, URA 1156 CNRS, Institut Gustave Roussy, Villejuif, France.
Fizazi
K
K
Walczak
V
V
Tetaud
C
C
Wiels
J
J
Tursz
T
T
Busson
P
P
D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000821:virology; D006028:Glycosphingolipids / Q000378:metabolism*; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000821:virology*; D051379:Mice; D008819:Mice, Nude; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000378:metabolism*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D006028:Glycosphingolipids; D014763:Viral Matrix Proteins