[Histological study of micrognathic development in mouse fetus induced by 3,3-dimethyl-1-phenyltriazene].
The purpose of this study was to investigate the influence of 3,3-dimethyl-1-phenyltriazene (DMPT) on development of craniofacial structures in mouse fetus. (1) Pregnant C57BL/6 mice were treated with 30 mg/kg DMPT injection on days 10.5 approximately 13.5 of gestation. The fetuses were analyzed on day 15.0. The incidence of micrognathia was formed 100% in the treated group on days 10.5 and 11.0, and then decreased remarkably after day 11.5. This result suggested that the critical period of micrognathic development is days 10.5 approximately 11.0. (2) The fetuses, injected 30 mg/kg DMPT on day 10.5, were sacrificed on days 11.0 approximately 18.0. Their heads were used for the following histomorphological analyses: 1. clearing and Alcian blue-alizarin red double staining and 2. lateral graphic reconstruction using serial frontal sections. Dysmorphology of the anterior portion of Meckel's and nasal septal cartilage became evident after day 13.0, however, nasal capsule cartilage was hardly affected. It was concluded that hypoplastic Meckel's cartilage, caused by the administration of DMPT, induced mandibular dysmorphogenesis.
Komiya
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Department of Maxillo-Facial Orthognathics, Graduate School of Dentistry, Tokyo Medical and Dental University.
D000818:Animals; D051379:Mice; D008810:Mice, Inbred C57BL; D008844:Micrognathism / Q000139:chemically induced* / Q000196:embryology; D009153:Mutagens; D014226:Triazenes
D009153:Mutagens; D014226:Triazenes; C008848:1-phenyl-3,3-dimethyltriazene