title: Ultrastructural localization of galanin and galanin receptors in the guinea pig median eminence.
abstract: The aim of this work was to compare the localization of galanin and galanin receptors in the guinea pig median eminence at the light and electron microscopic level. Concerning galanin the highest labeling was shown in the external part of the median eminence. At the ultrastructural level, galanin immunoreactivity was observed only in nerve terminals containing granular vesicles of approximately 120 nm in diameter. Light microscopic autoradiographs of semithin sections exhibited a moderate labeling in the external part of the median eminence. Galanin receptors were labeled in vitro on semithin sections (2 microm) using the highly specific radioligand [125I]galanin. Ultrastructural data showed that most of galanin binding sites overlaid membrane appositions between nerve terminals and also between nerve terminal and tanycyte. By considering the percentages in the distribution of the binding it appeared that galanin receptors were located on some nerve ending membranes. Our observations were not really in favor of a presence of receptors in tanycytes. The presence of galanin nerve endings in the external part suggests that like in the rat the peptide may have a direct hypophysiotrophic role. In contrast, the occurrence of numerous binding sites gives additional arguments in favor of a local action (paracrine and/or autocrine) of galanin occurring via galanin receptors located essentially on the pericapillary nerve terminals in the guinea pig median eminence.
authors.affiliation: Unité 422 INSERM, Lille, France.
authors.forename: I
authors.initials: I
authors.lastname: Dutriez
authors.forename: I
authors.initials: I
authors.lastname: Lagny-Pourmir
authors.forename: J
authors.initials: J
authors.lastname: Epelbaum
authors.forename: J C
authors.initials: JC
authors.lastname: Beauvillain
mesh_terms: D000818:Animals; D001345:Autoradiography; D019004:Galanin / Q000032:analysis*; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques; D008297:Male; D008473:Median Eminence / Q000737:chemistry*; D016253:Microscopy, Immunoelectron; D051381:Rats; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis*
chemical_list: D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone; D019004:Galanin