title: Evaluation of donor skin disinfection methods.
abstract: BACKGROUND
  Because most bacteria isolated from contaminated platelet concentrates are thought to originate from the donor's skin, the efficacy of four methods of skin disinfection was compared.
  
  
  METHODS
  Contact plates were used for antecubital skin cultures after they were demonstrated to be easier to use and at least as sensitive as a swab system. One antecubital fossa of each subject was disinfected by a standard method, the use of a povidone-iodine swabstick containing 0.75-percent available iodine followed by the use of a povidone-iodine swabstick containing 1-percent available iodine. The other arm was disinfected with either a 70-percent isopropyl alcohol scrub followed by an ampoule of 2-percent iodine tincture (Group 1; n = 126); a green-soap sponge followed by a 70-percent isopropyl alcohol swab, used for donors who are allergic to iodine (Group 2; n = 30); or a 0.5-percent chlorhexidine gluconate and 70-percent isopropyl alcohol sponge followed by an ampoule of 0.5-percent chlorhexidine gluconate and 70-percent isopropyl alcohol (Group 3; n = 40). Contact plate cultures were done before and after disinfection, and colonies counted after a 48-hour 37 degrees C incubation period.
  
  
  RESULTS
  Similar numbers of bacteria grew from both antecubital fossae of the same subject before disinfection (p = 0.71). Compared to the standard povidoneiodine method, isopropyl alcohol and tincture of iodine resulted in significantly less bacterial growth (p < 0.001), the green soap and isopropyl alcohol method resulted in significantly more bacterial growth (p < 0.001), and the chlorhexidine gluconate and isopropyl alcohol method resulted in similar amounts of bacterial growth (p > 0.3).
  
  
  CONCLUSIONS
  Isopropyl alcohol scrub followed by iodine tincture is more efficacious than povidone-iodine as measured by contact plate cultures. For donors who are allergic to iodine, chlorhexidine gluconate and isopropyl alcohol is more efficacious than green soap and isopropyl alcohol.
authors.affiliation: Blood Service, Transfusion Centre of Québec at Montréal, Canadian Red Cross Society, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Goldman
authors.forename: G
authors.initials: G
authors.lastname: Roy
authors.forename: N
authors.initials: N
authors.lastname: Fréchette
authors.forename: F
authors.initials: F
authors.lastname: Décary
authors.forename: L
authors.initials: L
authors.lastname: Massicotte
authors.forename: G
authors.initials: G
authors.lastname: Delage
mesh_terms: D000433:1-Propanol / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D015169:Colony Count, Microbial; D005069:Evaluation Studies as Topic; D006801:Humans; D007455:Iodine / Q000494:pharmacology; D018962:Phlebotomy; D011206:Povidone-Iodine / Q000494:pharmacology; D012867:Skin / Q000382:microbiology*
chemical_list: D000891:Anti-Infective Agents, Local; D011206:Povidone-Iodine; D007455:Iodine; D000433:1-Propanol