Interleukin-1 receptor type I mRNA levels in brain regions from male and female rats.
Interleukin-1 (IL-1) induces a variety of neurological manifestations by direct action in the central nervous system (CNS). The IL-1 receptor type I (IL-1RI) mediates IL-1 signalling. In the present study, the steady-state content of IL-1RI mRNA was determined by a sensitive RNase protection assay in brain regions obtained from normal male and nonestrous female Wistar rats. The results show that brain regions differ in IL-1RI mRNA content. Highest levels of IL-1RI mRNA were detected in the male and female cerebral cortex. High levels of IL-1RI mRNA were also observed in the brain stem and its structures, and the cerebellum. Male and female rats exhibited similar differential profile of IL-1RI mRNA levels in the frontal cortex, hippocampus, hypothalamus, and cerebellum. The present data on brain distribution of IL-1RI mRNA levels suggest that distinct brain regions may depend differentially on the IL-1 system.
Gayle
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Division of Molecular Biology, School of Life and Health Sciences, University of Delaware, Newark 19716-2590, USA.
Ilyin
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SE
Plata-Salamán
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CR
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005260:Female; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017472:Receptors, Interleukin-1 / Q000378:metabolism*; D012727:Sex Characteristics
D012333:RNA, Messenger; D017472:Receptors, Interleukin-1