A system for isolation, transport and storage of herpes simplex viruses.
A major difficulty with diagnostic virus isolation concerns the relative thermolability of certain viruses, e.g. herpes simplex virus type 2, which may, therefore, lose infectivity during transport to the laboratory. This study describes a system of virus isolation and transport, which depends on direct inoculation at the bedside or clinic, to a monolayer or suspension of susceptible cells with subsequent incubation for 10 h at approximately 32 degrees C, whereupon the newly synthesised virus becomes very stable if the cells are subsequently maintained at room temperature. This system was found to increase the sensitivity of isolation of herpes simplex virus, particularly under conditions of asymptomatic virus excretion or if there was significant delay in transportation of clinical samples to the virus laboratory. It is envisaged that this system will allow clinical self-sampling by the patient with application to epidemiological surveys in both the developed and underdeveloped world.
Skinner
G R
GR
Department of Infection, University of Birmingham, UK. g.r.b.skinner@bham.ac.uk
Billstrom
M A
MA
Randall
S
S
Ahmad
A
A
Patel
S
S
Davies
J
J
Deane
A
A
D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002999:Clone Cells; D006224:Cricetinae; D005260:Female; D006558:Herpes Genitalis / Q000175:diagnosis; D007668:Kidney / Q000166:cytology / Q000821:virology; D018139:Simplexvirus / Q000254:growth & development / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000502:physiology