Feulgen staining of intact plant tissues for confocal microscopy.
A method was developed to prepare plant structures for confocal laser scanning microscopy by combining Feulgen staining with pararosaniline and embedding in LR White(TM). This procedure preserves intact, delicate structures for three-dimensional imaging without loss from sectioning or squashing, and the slides can be viewed several times without serious photo-bleaching.
Braselton
J P
JP
Department of Environmental and Plant Biology, Ohio University, Athens, 45701-2979, USA.
Wilkinson
M J
MJ
Clulow
S A
SA
D000180:Acrylic Resins; D004396:Coloring Agents; D018613:Microscopy, Confocal / Q000379:methods; D010944:Plants / Q000737:chemistry / Q000648:ultrastructure*; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*; D016610:Tissue Embedding
D000180:Acrylic Resins; D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; C048707:LR white