Consequences of gamma-irradiation on inflammatory cytokine regulation in human monocytes/macrophages.
Inflammation is a frequent result of radiation damage, especially after therapeutic irradiation. Monocytes and macrophages play an important role in inflammatory cytokine secretion. However, knowledge of monocyte/ macrophage involvement in the pathogenesis of inflammation is still incomplete. In this study, we investigated the inflammatory cytokine regulation after in vitro gamma-irradiation of monocytes/macrophages. Semiquantitative RT-PCR after irradiation revealed no significant induction of interleukin 1 beta or tumour necrosis factor-alpha mRNA expression. When induction was observed, for only two donors out of seven, only interleukin 6 gene expression was affected by irradiation, with a 5-14-fold increase in level according to the donor, 2h after irradiation compared with sham-irradiated cells. For only one of seven donors tested, monocytes/macrophages responded to 10-Gy gamma-rays by releasing inflammatory cytokines.
Pons
I
I
Service de Neurovirologie, Institut Paris-Sud sur les Cytokines, CEA, DSV/DRM, Fontenay aux Roses, France.
Gras
G
G
Courberand
S
S
Benveniste
O
O
Dormont
D
D
D002469:Cell Separation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects; D006801:Humans; D007249:Inflammation / Q000473:pathology / Q000503:physiopathology; D007375:Interleukin-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008264:Macrophages / Q000528:radiation effects*; D009000:Monocytes / Q000528:radiation effects*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha