The structure of free radical metabolites detected by EPR spin trapping and mass spectroscopy from halocarbons in rat liver microsomes.
Electron impact (EI) tandem mass spectrometry (MS/MS) combined with EPR spin trapping was used to detect and identify the free radical metabolites of various halocarbons in rat liver microsomal dispersions. EPR spectra of the spin adducts of radical metabolites derived from fluorine-containing halocarbons display fluorine hyperfine splitting, which can be used as proof for the identification of this kind of halocarbon-derived free radical spin adduct. For halocarbons without fluorine atoms, MS/MS was found to be a very useful and simple method for the detection and identification of the structures of halocarbon-derived spin adducts from radical metabolites. The molecular ions from spin adducts of these halocarbon-derived free radical intermediates were observed for the first time by scanning the precursor ion spectrum of m/z 57. These assignments were further confirmed by the use of perdeuterated tert-butyl PBN which provides the precursor ion spectrum of m/z 66.
Sang
H
H
Free Radical Biology and Aging Research program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
Janzen
E G
EG
Poyer
J L
JL
McCay
P B
PB
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D005461:Fluorine / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006846:Hydrocarbons, Halogenated / Q000737:chemistry* / Q000378:metabolism*; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism*; D015394:Molecular Structure; D051381:Rats; D013113:Spin Labels
D005609:Free Radicals; D006846:Hydrocarbons, Halogenated; D013113:Spin Labels; D005461:Fluorine