title: Distribution and regulation of esterases that hydrolyze methyl farnesoate in Homarus americanus and other crustaceans.
abstract: Ester hydrolysis of methyl farnesoate (MF) by crustacean tissue homogenates was measured using the substrate [3H]MF in a radiochemical partition assay. Tissues were obtained from the lobster Homarus americanus, penaeid shrimp Sicyonia ingentis, thalanassid shrimp Callianassa californiensis, sand crab Emerita analoga, and spider crab Pugettia producta. The greatest specific activities were recovered from the hepatopancreas (239 to 11,500 pmol MF/min-mg total protein). Hepatopancreatic homogenates of C. californiensis were significantly more active than homogenates from the other species. In the lobster, esterases that hydrolyze MF were associated with lipid storage (R) cells of the hepatopancreas. Enzyme activity of lobster larval homogenates increased 1.5-fold during the second stage of development. The rate of MF hydrolysis by esterases extracted from the juvenile lobster hepatopancreas could not be correlated with molt stage or sex and was not significantly influenced by eyestalk ablation, mandibular organ ablation, or MF injection.
authors.affiliation: Bodega Marine Laboratory, University of California, Bodega Bay 94923, USA.
authors.forename: E
authors.initials: E
authors.lastname: Homola
authors.forename: E S
authors.initials: ES
authors.lastname: Chang
mesh_terms: D000818:Animals; D003445:Crustacea / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D006868:Hydrolysis; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008121:Nephropidae / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology / Q000378:metabolism; D013045:Species Specificity; D017186:Titrimetry
chemical_list: D005231:Fatty Acids, Unsaturated; C032429:methyl farnesoate; D004950:Esterases