title: The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames.
abstract: A 54,719 bp fragment from the right arm of Saccharomyces cerevisiae chromosome XV has been sequenced from the inserts of two cosmids (pEOA213 and pEOA217). The computer analysis of this sequence has revealed the presence of eight known genes (CKA2, CYC1, ALG8, TCM1, TMP1, UFE1, RTS2 and ASE1) and four open reading frames (ORFs) with strong homologies with known yeast genes (MLP1, SIS2 and HBS1 and the allantoin permease). The characteristics of the other ORFs and of the corresponding proteins do not allow postulation of a precise function. Several have features reminiscent of cytoskeleton or motor elements (keratin-like, myosin-like) and several others have characteristics of proteins which interact with DNA (extremely basic, b-Zip structure and/or acidic domains). Two tRNAs (tRNA(Lys) and tRNA(Pro)) have also been identified on this fragment. Many of these ORFs present similarities with ORFs located on chromosome XI, indicating some information reshuffling between the two chromosomal fragments.
authors.affiliation: Laboratoire de Génétique Moléculaire, Institut de Génétique et Microbiologie (URA 1354 du CNRS), Université Paris-Sud, Orsay, France.
authors.forename: M
authors.initials: M
authors.lastname: Valens
authors.forename: C
authors.initials: C
authors.lastname: Bohn
authors.forename: B
authors.initials: B
authors.lastname: Daignan-Fornier
authors.forename: V D
authors.initials: VD
authors.lastname: Dang
authors.forename: M
authors.initials: M
authors.lastname: Bolotin-Fukuhara
mesh_terms: D000595:Amino Acid Sequence; D001482:Base Composition; D015825:Chromosomes, Fungal / Q000235:genetics*; D003062:Codon / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D012357:RNA, Transfer, Lys / Q000235:genetics*; D012361:RNA, Transfer, Pro / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D003062:Codon; D012331:RNA, Fungal; D012357:RNA, Transfer, Lys; D012361:RNA, Transfer, Pro