title: Up-regulation of human epidermal Langerhans' cell B7-1 and B7-2 co-stimulatory molecules in vivo by solar-simulating irradiation.
abstract: Ultraviolet (UV) radiation impairs cutaneous immune functions and induces antigen-specific tolerance both locally at the irradiated skin site, as well as at distant skin sites and systemically. It has been postulated that in the local model, altered Langerhans' cells (LC) provide tolerogenic signals, and studies in vitro have indicated that UV radiation may down-regulate the expression of co-stimulatory molecules on the surface of these cells. To examine the effect of UV radiation on LC co-stimulatory molecules in vivo, we irradiated human volunteers with erythematogenic doses of solar-simulating UV radiation (SSR), and analyzed the expression of cell surface markers in dermatome skin samples obtained 1-72 h post-irradiation. For flow cytometric analysis, epidermal cell (EC) suspensions were prepared and double labeled with monoclonal antibodies against CD1a or HLA-DR, and B7-1 (CD80), B7-2 (CD86), ICAM-1 (CD54), ICAM-3 (CD50), LFA-3 (CD58), E-cadherin, or integrin-beta4 (CD104). In unirradiated control skin samples, keratinocytes (KC) expressed high levels of E-cadherin. LC expressed high levels of both E-cadherin and ICAM-3, and low levels of B7-2, LFA-3, ICAM-1, and integrin-beta4. Following SSR, a triphasic reaction pattern was seen: an immediate, down-regulatory phase prevailing 2-6 h post-irradiation, when the number of DR+ and CD1a+ cells were temporarily reduced; a delayed, up-regulatory phase in which the number of LC was increased and the expression intensities of CD1a, HLA-DR, B7-1, and B7-2 were strongly up-regulated, maximally evident 12-24 h after irradiation, but no more seen at 48 h; and a late phase at 72 h, in which an influx of monocytes and a concomitant rise in DR+ cells was recorded. We conclude that to understand real-life cutaneous UV immunology, studies in vitro need to be complemented with studies in vivo. In the case of LC, the effects of erythematogenic UV radiation in vivo on human LC B7 co-stimulatory molecules include an up-regulatory stage.
authors.affiliation: Department of Dermatology, University of Turku, Finland.
authors.forename: J K
authors.initials: JK
authors.lastname: Laihia
authors.forename: C T
authors.initials: CT
authors.lastname: Jansen
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis / Q000528:radiation effects*; D018949:Antigens, CD1 / Q000528:radiation effects; D018122:B7-1 Antigen / Q000096:biosynthesis / Q000528:radiation effects*; D051940:B7-2 Antigen; D002452:Cell Count / Q000528:radiation effects; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism* / Q000528:radiation effects*; D006684:HLA-DR Antigens / Q000528:radiation effects; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000528:radiation effects*; D013472:Sunlight; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000276:immunology* / Q000528:radiation effects
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins