title: Analysis of kanamycin sulfate by liquid chromatography with pulsed electrochemical detection.
abstract: The analysis of kanamycin sulfate by liquid chromatography using a column packed with poly(styrene-divinylbenzene) and pulsed electrochemical detection on a gold electrode is described. A two-step gradient was necessary to obtain a good separation together with a reasonable analysis time of maximum 45 min. The mobile phases consisted of an aqueous solution of 20 g/l or 60 g/l sodium sulfate, 1.3 g/l sodium octanesulfonate and 50 ml/l 0.2 M phosphate buffer pH 3.0. Sodium hydroxide was added post-column. The influence of the different chromatographic parameters on the separation was investigated. A number of commercial samples were analyzed using this method. Besides the previously reported impurities, such as kanamycins B and C, two other impurities were separated, one of which is called kanamycin D. In total, eight components were separated.
authors.affiliation: Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Adams
authors.forename: J
authors.initials: J
authors.lastname: Dalle
authors.forename: E
authors.initials: E
authors.lastname: De Bie
authors.forename: I
authors.initials: I
authors.lastname: De Smedt
authors.forename: E
authors.initials: E
authors.lastname: Roets
authors.forename: J
authors.initials: J
authors.lastname: Hoogmartens
mesh_terms: D002853:Chromatography, Liquid; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007475:Ion Exchange Resins; D007612:Kanamycin / Q000032:analysis*; D011137:Polystyrenes
chemical_list: D007202:Indicators and Reagents; D007475:Ion Exchange Resins; D011137:Polystyrenes; D007612:Kanamycin; C003771:Amberlite XAD-2 resin