title: Yeast colony size reflects YAC copy number.
abstract: A novel strategy for separation of co-cloned YACs was developed. For this, yeast cells were grown under non-selective conditions to allow the mitotic loss of multiple YACs. Yeast colonies of different size appear on 'drop-out' selection plates with small clones consistently containing a single-copy YAC. Different auxotrophic marker genes can be used to separate co-cloned YACs or reduce their copy number, which is essential for most YAC-modification procedures.
authors.affiliation: Department of Development and Genetics, The Babraham Institute, Cambridge CB2 4AT, UK. andrei.popov@bbsrc.ac.uk
authors.forename: A V
authors.initials: AV
authors.lastname: Popov
authors.forename: C
authors.initials: C
authors.lastname: Bützler
authors.forename: M
authors.initials: M
authors.lastname: Brüggemann
mesh_terms: D015139:Blotting, Southern; D018244:Chromosomes, Artificial, Yeast / Q000648:ultrastructure; D003001:Cloning, Molecular / Q000379:methods*; D003470:Culture Media; D005821:Genetic Techniques; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*
chemical_list: D003470:Culture Media