Crystal structure of chicken riboflavin-binding protein.
The crystal structure of chicken egg white riboflavin-binding protein, determined to a resolution of 2.5 A, is the prototype of a family that includes other riboflavin- and folate-binding proteins. An unusual characteristic of these molecules is their high degree of cross-linking by disulfide bridges and, in the case of the avian proteins, the presence of stretches of highly phosphorylated polypeptide chain. The structure of chicken egg white riboflavin-binding protein is characterized by a ligand-binding domain and a phosphorylated motif. The ligand-binding domain has a fold that appears to be strongly conditioned by the presence of the disulfide bridges. The phosphorylated motif, essential for vitamin uptake, is made up of two helices found before and after the flexible phosphorylated region. The riboflavin molecule binds to the protein with the isoalloxazine ring stacked in between the rings of Tyr75 and Trp156. This geometry and the proximity of other tryptophans explain the fluorescent quenching observed when riboflavin binds to the protein.
Monaco
H L
HL
Department of Genetics, University of Pavia, Italy.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002645:Chickens; D003196:Computer Graphics; D018360:Crystallography, X-Ray; D004220:Disulfides; D005260:Female; D005415:Flavins; D058973:Folate Receptors, GPI-Anchored; D006801:Humans; D008024:Ligands; D026901:Membrane Transport Proteins; D008958:Models, Molecular; D008961:Models, Structural; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011956:Receptors, Cell Surface; D012256:Riboflavin / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D004220:Disulfides; D005415:Flavins; D058973:Folate Receptors, GPI-Anchored; D008024:Ligands; D026901:Membrane Transport Proteins; D011956:Receptors, Cell Surface; C019079:riboflavin-binding protein; C008173:isoalloxazine; D012256:Riboflavin