title: Biochemical characterization of microcystin toxicity in rainbow trout (Oncorhynchus mykiss).
abstract: The kinetics and biochemical effects of microcystins in rainbow trout were studied with freeze-dried toxic cells of Microcystis aeruginosa, strain PCC 7806. Following in vivo exposure the changes in liver histology were observed over a 72 hr period and the absorption of microcystins from the gastrointestinal tract into the blood and liver, as well as the inhibition of hepatic protein phosphatase 1 and 2A activities, were recorded using the protein phosphatase inhibition assay. The interaction between microcystins and trout liver phosphatases was further tested in vitro using the protein phosphatase inhibition assay. The in vivo experiments demonstrated a high organotropy of microcystins for the liver, where rapid and total inhibition of protein phosphatase 1 and 2A activity was observed. Maximal inhibition of phosphatases was observed 3 hr after gavage. At that time-point, approximately 63% of the toxin present in the liver was refractive to detection via the phosphatase inhibition assay and therefore most likely covalently bound to cellular proteins. The inhibition of hepatic protein phosphatases 1 and 2A proved to be transient only, as a progressive increase in phosphatase activity was observed beginning 12 hr after gavage of the fish, reaching approximately 50% of the control activity at 72 hr. In contrast, liver damage continued to progress despite this renewed protein phosphatase activity.
authors.affiliation: Institute of Toxicology, Federal Institute of Technology, Schwerzenbach, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Tencalla
authors.forename: D
authors.initials: D
authors.lastname: Dietrich
mesh_terms: D000818:Animals; D000458:Cyanobacteria; D004791:Enzyme Inhibitors / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D052998:Microcystins; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010456:Peptides, Cyclic / Q000493:pharmacokinetics / Q000633:toxicity*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D054645:Protein Phosphatase 1
chemical_list: D004791:Enzyme Inhibitors; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1