title: Mutants of Arabidopsis thaliana hypersensitive to DNA-damaging treatments.
abstract: A simple screening method was developed for the isolation of Arabidopsis thaliana mutants hypersensitive to X-ray irradiation. The root meristem was used as the target for irradiation with sublethal doses of X rays, while protection of the shoot meristem by a lead cover allowed the rescue of hypersensitive individuals. We isolated nine independent X-ray-hypersensitive mutants from 7000 M2 seedlings. Analysis of three chosen mutants (xrs4, xrs9 and xrs11) showed that alterations in single recessive alleles are responsible for their phenotypes. The mutations are not allelic but linked and map to chromosome 4, suggesting mutations in novel genes as compared to previously mapped mutant alleles. Importantly, hypersensitivity to X rays was found to correlate with hypersensitivity to the DNA-alkylating agent mitomycin C, which provokes interstrand crosslinks, and/or to methyl methanesulfonate, which is known as a radiomimetic chemical. These novel phenotypes suggest that the mutants described here are altered in the repair of DNA damage, most probably by recombinational repair.
authors.affiliation: Friedrich Miescher Institute, Basel, Switzerland. masson@fmi.ch
authors.forename: J E
authors.initials: JE
authors.lastname: Masson
authors.forename: P J
authors.initials: PJ
authors.lastname: King
authors.forename: J
authors.initials: J
authors.lastname: Paszkowski
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D002874:Chromosome Mapping; D004249:DNA Damage; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D010641:Phenotype
chemical_list: D009153:Mutagens