Identification of alpha-fodrin as a candidate autoantigen in primary Sjögren's syndrome.
It is unclear whether organ-specific autoantigens are critical for the development of primary Sjögren's syndrome (SS). A 120-kilodalton organ-specific autoantigen was purified from salivary gland tissues of an NFS/sld mouse model of human SS. The amino-terminal residues were identical to those of the human cytoskeletal protein alpha-fodrin. The purified antigen induced proliferative T cell responses and production of interleukin-2 and interferon-gamma in vitro. Neonatal immunization with the 120-kilodalton antigen prevented the disease in mice. Sera from patients with SS reacted positively with purified antigen and recombinant human alpha-fodrin protein, whereas those from patients with systemic lupus erythematosus and rheumatoid arthritis did not. Thus, the immune response to 120-kilodalton alpha-fodrin could be important in the initial development of primary SS.
Haneji
N
N
Department of Pathology, Tokushima University School of Dentistry, 3 Kuramotocho, Tokushima 770, Japan.
Nakamura
T
T
Takio
K
K
Yanagi
K
K
Higashiyama
H
H
Saito
I
I
Noji
S
S
Sugino
H
H
Hayashi
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis / Q000276:immunology; D001324:Autoantigens / Q000276:immunology* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000276:immunology* / Q000302:isolation & purification; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006801:Humans; D007114:Immunization; D015151:Immunoblotting; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D008840:Microfilament Proteins / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012469:Salivary Glands / Q000276:immunology; D012859:Sjogren's Syndrome / Q000276:immunology* / Q000517:prevention & control; D013601:T-Lymphocytes / Q000276:immunology; D013049:Spectrin
D001323:Autoantibodies; D001324:Autoantigens; D002352:Carrier Proteins; D007371:Interferon-gamma; D007376:Interleukin-2; D008840:Microfilament Proteins; D011993:Recombinant Fusion Proteins; D013049:Spectrin