Demonstration of antibodies against human papillomavirus type-11 E6 and L2 proteins in patients with recurrent respiratory papillomatosis.
Recurrent respiratory papillomatosis (RRP) is highly prevalent in Thailand, with the human papillomavirus (HPV) type-11 being the most widespread. In this study, we isolated the HPV type-11 (HPV-11) genome from subjects with RRP and subcloned the E6 and L2 open reading frames (ORFs) with the expression vectors pEX1 and pEX3. The recombinant E6/beta-gal and L2/beta-gal fusion proteins were expressed in E. coli. Using the recombinant proteins, we demonstrated the presence of antibodies against HPV-11 E6 and L2 in RRP patients by Western blot analysis. The prevalence of seropositivity for HPV-11 E6 and L2 were 5% (1/20) and 10% (2/20), respectively. Although RRP is caused by infection on the mucosal surface, it appears that an immune response occurs against viral proteins expressed in the epithelial lesions.
Sameshima
A
A
Department of Otolaryngology, Faculty of Medicine, Kagoshima University, Japan.
Fujiyoshi
T
T
Pholampaisathit
S
S
Ushikai
M
M
Kono
M
M
Antarasena
S
S
Fukuda
K
K
Furuta
S
S
Sonoda
S
S
Ohyama
M
M
D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D015344:DNA Probes, HPV; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D006801:Humans; D007223:Infant; D007822:Laryngeal Neoplasms / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis* / Q000276:immunology; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology*; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000276:immunology; D013785:Thailand; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000276:immunology
D000914:Antibodies, Viral; D015344:DNA Probes, HPV; D009856:Oncogene Proteins, Viral