Effect of immunization with an inactivated gp120-depleted HIV-1 immunogen on beta-chemokine and cytokine production in subjects with HIV-1 infection.
OBJECTIVE
To measure beta-chemokine and cytokine production in HIV-1-infected subjects undergoing treatment with HIV-1 immunogen (REMUNE).


METHODS
Open label treatment study.


METHODS
beta-Chemokine and cytokine production in peripheral blood mononuclear cell (PBMC) culture.


RESULTS
Interferon-gamma production (p = 0.04) and lymphocyte proliferation (p = 0.001) to HIV-1 antigen-stimulated PBMCs increased after immunization with the HIV-1 immunogen. A correlation was demonstrated after immunization between HIV-1 antigen-stimulated lymphocyte proliferation and interferon-gamma levels (r = 0.53, p = 0.04). No significant change after immunization was seen for interleukin-4 production. A significant increase in mean levels of HIV-1 antigen-stimulated RANTES (i.e., regulated upon, activation normal T-cell expressed and secreted), was evident 1 month after immunization (p = 0.002) and remained elevated 3 months after immunization. RANTES production was decreased in CD8-depleted PBMC cultures. Mean serum HIV-1 RNA copy numbers and CD4 cell counts remained stable after immunization (p > 0.5). A correlation was demonstrated between HIV-1 antigen-stimulated interferon-gamma and RANTES production (r = 0.54, p = 0.002).


CONCLUSIONS
This report describes an augmentation of beta-chemokines and TH1-type cytokines from PBMCs after immunization with the HIV-1 immunogen.
Moss
R B
RB
Immune Response Corporation, Carlsbad, California 92008, USA.
Trauger
R J
RJ
Giermakowska
W K
WK
Turner
J L
JL
Wallace
M R
MR
Jensen
F C
FC
Richieri
S P
SP
Ferre
F
F
Daigle
A E
AE
Duffy
C
C
Theofan
G
G
Carlo
D J
DJ
D018791:CD4 Lymphocyte Count; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000032:analysis; D018925:Chemokines / Q000096:biosynthesis*; D016207:Cytokines / Q000096:biosynthesis*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1; D006801:Humans; D007114:Immunization; D007371:Interferon-gamma / Q000032:analysis; D008213:Lymphocyte Activation; D013997:Time Factors
D018946:Chemokine CCL5; D018925:Chemokines; D016207:Cytokines; D015699:HIV Envelope Protein gp120; D007371:Interferon-gamma