title: Description of an albumin binding activity for Streptococcus suis serotype 2.
abstract: This study was undertaken to investigate the binding activity of Streptococcus suis serotype 2 to albumin. Using flow cytometry we observed a binding activity of S. suis to albumin for virulent as well as for avirulent isolates. Western immunoblots analysis revealed that a 39-kDa S. suis protein was responsible, at least in part, for this binding activity. This protein showed high N-terminal homology (95.6% for the first 23 residues) with a group A Streptococcus glyceraldehyde-3-phosphate dehydrogenase. Furthermore, the addition of albumin to the culture broth resulted in an increase in the virulence of S. suis strains in mice. These results suggest that an interaction with albumin could play a role in the pathogenesis of S. suis serotype 2 infections.
authors.affiliation: Laboratoire d'hygiène vétérinaire et alimentaire, Agriculture et Agro-alimentaire Canada, St-Hyacinthe, Québec, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Quessy
authors.forename: P
authors.initials: P
authors.lastname: Busque
authors.forename: R
authors.initials: R
authors.lastname: Higgins
authors.forename: M
authors.initials: M
authors.lastname: Jacques
authors.forename: J D
authors.initials: JD
authors.lastname: Dubreuil
mesh_terms: D000418:Albumins / Q000276:immunology / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D005434:Flow Cytometry; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D013290:Streptococcal Infections / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000235:genetics; D017011:Streptococcus suis / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
chemical_list: D000418:Albumins; D001426:Bacterial Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases