Effects of extracts from Populus tremula L., solidago virgaurea L. and Fraxinus excelsior L. on various myeloperoxidase systems.
Extracts from Populus tremula, Solidago virgaurea and Fraxinus excelsior are used as anti-inflammatory drugs. The effects of these extracts on myeloperoxidase (MPO), an enzyme liberated by activated granulocytes and known to produce the destructive agent hypochloric acid, were investigated. Populus and Fraxinus inhibited this enzyme, Solidago was without effect. These results were obtained concordantly with four different MPO-assays (H2O2/MPO; X/XOD/MPO; activated PMN; and elastase/alpha 1-PI-MPO). Fractionation of Populus and Fraxinus extracts showed that this inhibition is due to several different compounds. Well known or wide spread substances as e.g. rutin, salicylic acid, chlorogenic acid etc. had no or only little effect on the enzyme.
von Kruedener
S
S
Lehrstuhl für Phytopathologie, Labor für angewandte Biochemie, Technische Universität München, Germany.
Schneider
W
W
Elstner
E F
EF
D000596:Amino Acids / Q000737:chemistry; D000598:Amino Acids, Cyclic; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry*; D005030:Ethylenes / Q000737:chemistry; D006861:Hydrogen Peroxide; D006997:Hypochlorous Acid / Q000737:chemistry; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D009195:Peroxidase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D013552:Swine; D014969:Xanthine Oxidase
D000596:Amino Acids; D000598:Amino Acids, Cyclic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005030:Ethylenes; D010936:Plant Extracts; C023863:1-aminocyclopropane-1-carboxylic acid; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide; D009195:Peroxidase; D014969:Xanthine Oxidase