In vitro techniques for studying drug metabolism.
A considerable amount of information has been obtained about human enzymes involved in drug metabolism. Cytochrome P450 is presented as a paradigm in order to illustrate the experimental techniques now available. In vitro assays can be done with tissue slices, microsomes, or even short-term cell cultures. In addition, recombinant enzymes can be produced in a variety of different vector systems. Information about catalytic selectivity regarding new drugs can be obtained, as well as important information about potential drug-drug interactions and bioavailability. Such studies play a role in the drug development process as well as metabolism and safety assessment.
Guengerich
F P
FP
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA.
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002478:Cells, Cultured / Q000378:metabolism; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D015195:Drug Design; D004790:Enzyme Induction / Q000502:physiology; D004791:Enzyme Inhibitors / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011110:Polymorphism, Genetic
D000906:Antibodies; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D004798:Enzymes; D004364:Pharmaceutical Preparations; D003577:Cytochrome P-450 Enzyme System