title: Identification of alpha-fodrin as a candidate autoantigen in primary Sjögren's syndrome.
abstract: It is unclear whether organ-specific autoantigens are critical for the development of primary Sjögren's syndrome (SS). A 120-kilodalton organ-specific autoantigen was purified from salivary gland tissues of an NFS/sld mouse model of human SS. The amino-terminal residues were identical to those of the human cytoskeletal protein alpha-fodrin. The purified antigen induced proliferative T cell responses and production of interleukin-2 and interferon-gamma in vitro. Neonatal immunization with the 120-kilodalton antigen prevented the disease in mice. Sera from patients with SS reacted positively with purified antigen and recombinant human alpha-fodrin protein, whereas those from patients with systemic lupus erythematosus and rheumatoid arthritis did not. Thus, the immune response to 120-kilodalton alpha-fodrin could be important in the initial development of primary SS.
authors.affiliation: Department of Pathology, Tokushima University School of Dentistry, 3 Kuramotocho, Tokushima 770, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Haneji
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Takio
authors.forename: K
authors.initials: K
authors.lastname: Yanagi
authors.forename: H
authors.initials: H
authors.lastname: Higashiyama
authors.forename: I
authors.initials: I
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Noji
authors.forename: H
authors.initials: H
authors.lastname: Sugino
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis / Q000276:immunology; D001324:Autoantigens / Q000276:immunology* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000276:immunology* / Q000302:isolation & purification; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006801:Humans; D007114:Immunization; D015151:Immunoblotting; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D008840:Microfilament Proteins / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012469:Salivary Glands / Q000276:immunology; D012859:Sjogren's Syndrome / Q000276:immunology* / Q000517:prevention & control; D013601:T-Lymphocytes / Q000276:immunology; D013049:Spectrin
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D002352:Carrier Proteins; D007371:Interferon-gamma; D007376:Interleukin-2; D008840:Microfilament Proteins; D011993:Recombinant Fusion Proteins; D013049:Spectrin