A distinct profile of six soluble adhesion molecules (ICAM-1, ICAM-3, VCAM-1, E-selectin, L-selectin and P-selectin) in rheumatoid arthritis.
Soluble forms of ICAM-1, VCAM-1, E-selectin, L-selectin, P-selectin and, more recently, ICAM-3 are known to exist in human serum and have elevated levels in numerous diseases. Previous studies have demonstrated that in rheumatoid arthritis (RA) the levels of circulating sICAM-1 and sE-selectin are elevated relative to healthy controls. We have compared the serum profiles of these six soluble adhesion molecules in patients with RA (n = 22) to those seen in healthy controls (n = 10) using sandwich ELISA. In the patients, there were significant elevations of serum sICAM-1 (P < 0.0001), sICAM-3 (P = 0.0327), sVCAM-1 (P = 0.0025), sL-selectin (P = 0.0194) and sP-selectin (P = 0.0025), but not E-selectin (P = 0.0672). However, only sP-selectin was found to correlate with disease activity in the patients (r = 0.461, P < 0.05). Thus, there is a distinct profile of soluble adhesion molecules in RA of which only sP-selectin correlates with disease activity.
Littler
A J
AJ
Cell Adhesion Laboratory, John Radcliffe Hospital, Headington, Oxford.
Buckley
C D
CD
Wordsworth
P
P
Collins
I
I
Martinson
J
J
Simmons
D L
DL
D000328:Adult; D000368:Aged; D015703:Antigens, CD; D000943:Antigens, Differentiation; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000473:pathology; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000097:blood*; D019040:E-Selectin / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D019041:L-Selectin / Q000097:blood; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D012016:Reference Values; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D019040:E-Selectin; C483616:ICAM3 protein, human; D019007:P-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D002097:C-Reactive Protein; D007770:L-Lactate Dehydrogenase