title: Specification of posterior midbrain region in zebrafish neuroepithelium.
abstract: BACKGROUND
  The developing vertebrate nervous system displays a pronounced anterior-posterior (A-P) pattern, but the mechanism that generates this pattern is poorly understood. We examined through cell-transplantation experiments, when and how the cells in the zebrafish posterior midbrain acquire regional specificity along the A-P axis as shown by pax[b] gene expression.
  
  
  RESULTS
  Labelled donor cells from the presumptive midbrain region at various stages were transplanted into more anterior part of unlabelled host embryos of the same developmental stage, and the expression of pax[b] in the donor cells were examined by in situ hybridization. The results indicated that, in the cells from the presumptive midbrain region, expression of pax[b] was determined as early as the 55%-epiboly (6.5 h, early gastrulation) when the underlying hypoblastic layer reached the presumptive midbrain region. We also found that when transplanted heterotopically, anterior, but not posterior, hypoblast cells induced expression of pax[b] in the overlying ectoderm.
  
  
  CONCLUSIONS
  Expression of a midbrain specific gene is determined during early gastrulation and the hypoblastic layer plays an important role in this determination process.
authors.affiliation: Institute of Biological Science, University of Tsukuba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyagawa
authors.forename: H
authors.initials: H
authors.lastname: Amanuma
authors.forename: A
authors.initials: A
authors.lastname: Kuroiwa
authors.forename: H
authors.initials: H
authors.lastname: Takeda
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D004625:Embryo, Nonmammalian; D004848:Epithelium; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D017404:In Situ Hybridization, Fluorescence; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology*; D009024:Morphogenesis / Q000235:genetics; D015027:Zebrafish / Q000196:embryology