Effects of adrenalectomy and corticosterone replacement on diurnal [3H]citalopram binding in rat midbrain.
Corticosteroids modulate the expression and/or functions of several serotonin (5-hydroxytryptamine; 5-HT) receptors. Conversely, analyses of the effects of corticosteroids upon 5-HT reuptake systems have been scarce and contradictory. Herein, the diurnal rhythm of midbrain [3H]citalopram binding to 5-HT transporters was analysed in sham and 11 day adrenalectomised rats. In addition, adrenalectomised rats were either complemented or not with corticosterone pellets (12.5-200 mg). Analyses of body weight increases and plasma adrenocorticotropic and corticosterone levels indicated that the protocol allowed the stimulation of mineralocorticoid receptors (MRs; 12.5 mg pellets) or the stimulation of both MRs and glucocorticoid receptors (GRs; 50-200 mg pellets). However, besides the observation of a slight, but significant diurnal (corticosteroid-independent) rhythm in 5-HT transporter binding (morning > evening), it was found that neither adrenalectomy nor corticosteroid receptor stimulation affected midbrain [3H]citalopram binding.
Kulikov
A
A
INSERM CJF 94-05, INRA, Institut F. Magendie, Bordeaux, France.
Mormède
P
P
Chaouloff
F
F
D000315:Adrenalectomy; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001835:Body Weight; D002352:Carrier Proteins / Q000378:metabolism; D002940:Circadian Rhythm / Q000187:drug effects; D015283:Citalopram / Q000378:metabolism / Q000494:pharmacology*; D003345:Corticosterone / Q000494:pharmacology*; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D008636:Mesencephalon / Q000737:chemistry* / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009929:Organ Size; D010913:Pituitary-Adrenal System / Q000502:physiology; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D013950:Thymus Gland / Q000187:drug effects; D014316:Tritium
D000324:Adrenocorticotropic Hormone; D000893:Anti-Inflammatory Agents; D002352:Carrier Proteins; D015283:Citalopram; D003345:Corticosterone; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012701:Serotonin; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors; D014316:Tritium; C493232:Slc6a4 protein, rat