The effectiveness of inactivated, purified and concentrated experimental rabies vaccine for veterinary use: immunogenic activity.
The authors prepared an experimental, inactivated, concentrated and highly purified rabies vaccine from the strain Vnukovo-32/107. The purification and concentration (simultaneous--in one operation) was carried out by affinity chromatography. The content of rabies antigen in the vaccine was 4.256 UE/cm3 (determined by ELISA method). The authors used their own lipoid adjuvant--type oil in water--to resolve lyophilized vaccine to original volume. It is based on esters of fatty the acids. The effectiveness--immunogenic activity--of the experimental preparation was compared with that of commercial rabies vaccines and the reference vaccine by means of the NIH and NRLR methods. The results obtained (2.826 units/cm3 by the NIH and 3.085 units/cm3 by the NRLR method) confirmed that the method of affinity chromatography could be used to prepare a rabies vaccine, exhibiting effectiveness superior to commercial preparations available, and complying with the WHO criteria.
Süliová
J
J
University of Veterinary Medicine, Kosice, Slovak Republic.
Benísek
Z
Z
Svrcek
S
S
Durove
A
A
Ondrejka
R
R
D000818:Animals; D051379:Mice; D011818:Rabies / Q000517:prevention & control / Q000662:veterinary*; D011819:Rabies Vaccines / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology
D011819:Rabies Vaccines; D015164:Vaccines, Inactivated