Identification and characterization of homologues of the Exocyst component Sec10p.
The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% identity to a C. elegans protein found in the database. Northern and Western blot analyses indicate that hSec10 has a broad tissue distribution. Immunofluorescence staining of COS cells cotransfected with hSec10p and a mammalian Sec8p demonstrates that these two proteins have an identical distribution in the cell including a localization in the peripheral cytoplasm. These data suggest that hSec10p is a component of the mammalian counterpart of the yeast Exocyst complex essential for post-Golgi traffic.
Guo
W
W
Yale University School of Medicine, Department of Cell Biology, New Haven, CT 06510, USA.
Roth
D
D
Gatti
E
E
De Camilli
P
P
Novick
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D019556:COS Cells; D003001:Cloning, Molecular; D005089:Exocytosis; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008322:Mammals; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014162:Transfection; D033921:Vesicular Transport Proteins
D005656:Fungal Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C105132:SEC10 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins