Release of glucagon-like peptide-1 (GLP-1) by carbohydrates in the perfused rat ileum.
The effect of various carbohydrates on glucagon-like peptide-1 (GLP-1) release was studied in the in vivo perfused rat ileum. GLP-1 concentrations in the mesenteric venous effluent increased significantly after luminal perfusion with substrates of a sodium/glucose co-transporter (D-glucose, D-galactose, methyl-alpha D-glucoside, and 3-O-methyl-D-glucose). D-Fructose induced a sodium-independent release of GLP-1. Carbohydrates like 2-deoxy-D-glucose and N-acetyl-D-glucosamine, which are not substrates of a luminal sodium/glucose or fructose transporter, did not affect GLP-1 release. Since methyl-alpha D-glucoside is not a substrate of the basolateral glucose transport mechanism and 3-O-methyl-D-glucose is not metabolized within intestinal cells, it is concluded that intracellular metabolism of carbohydrates and intracellular removal are not essential to induce GLP-1 secretion in rats.
Ritzel
U
U
Department of Medicine, University of Göttingen, Germany.
Fromme
A
A
Ottleben
M
M
Leonhardt
U
U
Ramadori
G
G
D019325:3-O-Methylglucose / Q000494:pharmacology; D000117:Acetylglucosamine / Q000494:pharmacology; D000818:Animals; D003847:Deoxyglucose / Q000494:pharmacology; D005632:Fructose / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1; D005947:Glucose / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008757:Methylglucosides / Q000494:pharmacology; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009005:Monosaccharides / Q000494:pharmacology*; D010455:Peptides / Q000097:blood / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides; D010455:Peptides; D019325:3-O-Methylglucose; D005632:Fructose; C027020:methylglucoside; D052216:Glucagon-Like Peptide 1; D003847:Deoxyglucose; D005947:Glucose; D000117:Acetylglucosamine; D005690:Galactose