Fucosylation of synthetic oligosaccharides--plant xyloglucan fragments--by mammalian and amphibian fucosyltransferases.
Liver and oviduct of frogs Rana temporaria and Xenopus laevis were shown to possess the activity of beta-galactoside alpha 1-2-fucosyltransferase. Extracts of these tissues as well as porcine and rat submaxillary gland homogenates catalyze the fucosylation of synthetic plant xyloglucan fragments, the disaccharide D-Gal(beta 1-2)D-Xyl and the tetrasaccharide D-Gal(beta 1-2)D-Xyl(alpha 1-6)D-Glc(beta 1-4)D-Glc; the active preparations can be used for enzymatic synthesis of biologically active oligosaccharides.
Druzhinina
T N
TN
Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Sizova
O V
OV
Shibaev
V N
VN
D000818:Animals; D005643:Fucose / Q000378:metabolism*; D005647:Fucosyltransferases / Q000378:metabolism*; D005936:Glucans; D009844:Oligosaccharides / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D011896:Rana temporaria; D051381:Rats; D013363:Submandibular Gland / Q000201:enzymology / Q000378:metabolism; D013552:Swine; D014982:Xenopus laevis; D014990:Xylans
D005936:Glucans; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; D005643:Fucose; C029353:xyloglucan; D005647:Fucosyltransferases