title: Dog-DAT: a direct agglutination test using stabilized, freeze-dried antigen for the serodiagnosis of canine visceral leishmaniasis.
abstract: We have evaluated the use of an improved direct agglutination test (DAT) based on stable, freeze-dried antigen for the detection of anti-Leishmania antibodies in canine serum samples. With a cut-off value of 1:640, the sensitivity of the DAT was shown to be 100% and the specificity of the test was 98.8%.
authors.affiliation: Royal Tropical Institute, Department of Biomedical Research, Amsterdam, Netherlands. bo@mail.kit.support.nl
authors.forename: L
authors.initials: L
authors.lastname: Oskam
authors.forename: R J
authors.initials: RJ
authors.lastname: Slappendel
authors.forename: E G
authors.initials: EG
authors.lastname: Beijer
authors.forename: N C
authors.initials: NC
authors.lastname: Kroon
authors.forename: C W
authors.initials: CW
authors.lastname: van Ingen
authors.forename: S
authors.initials: S
authors.lastname: Ozensoy
authors.forename: Y
authors.initials: Y
authors.lastname: Ozbel
authors.forename: W J
authors.initials: WJ
authors.lastname: Terpstra
mesh_terms: D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D005069:Evaluation Studies as Topic; D005612:Freeze Drying; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D009426:Netherlands / Q000453:epidemiology; D012680:Sensitivity and Specificity; D014421:Turkey / Q000453:epidemiology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan