title: Presence of c-Myc protein in murine myeloid leukaemia cells during growth and after irradiation.
abstract: Myeloid leukaemia (ML) is strain specific for RFM mice, which were used in these experiments. We investigated the presence of c-myc protein during the growth of ML and after irradiation of leukaemic cells. Leukaemic spleen cells were investigated 9 (nonterminal phase NTP) or 12 days (terminal phase TP) after the injection of ML cells. Leukaemic cells of NTP were irradiated with X-rays or UV-light. c-Myc protein was detected by immunocytochemical method. c-Myc protein was expressed in 74.98% of spleen cells of healthy RFM mice. In the early period of leukaemia growth (NTP) only 14.33% of c-myc positive cells were found, as opposed to the terminal phase (TP) of leukaemia when 89.7% of c-myc positive cells were detected. These results indicated the connection of growth of ML and the presence of c-myc protein. If the spleen cells of NTP of leukaemia were irradiated by X-rays or UV-light, the number of cells which expressed c-myc protein was extremely increased.
authors.affiliation: Division of Biology and Medicine, Ruder Bosković Institute, Zagreb, Croatia.
authors.forename: M
authors.initials: M
authors.lastname: Popović-Hadzija
authors.forename: M
authors.initials: M
authors.lastname: Poljak-Blazi
authors.forename: K
authors.initials: K
authors.lastname: Pavelić
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D051379:Mice; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*
chemical_list: D016271:Proto-Oncogene Proteins c-myc