title: Identification of a protein-tyrosine phosphatase (SHP1) different from that associated with acid phosphatase in rat prostate.
abstract: Using [32P]poly(Glu,Tyr) as substrate, we have identified, for the first time, in the rat prostatic gland a protein-tyrosine phosphatase activity different from that associated with prostatic acid phosphatase. Concanavalin A-Sepharose 4B was used to separate the two protein-tyrosyl phosphatases activities. The activity retained by the lectin had characteristics of the prostatic acid phosphatase. It was sensitive to inhibition by PNPP and the optimum pH shifted towards physiological values when [32P]poly(Glu,Tyr) was used as substrate. However, the major protein-tyrosine phosphatase activity was not retained by the lectin, and corresponded, at least in part, to SHP1 as probed by the presence of the protein, its mRNA and the loss of PTPase activity after immunodepletion of SHP1. This enzyme is localized within the epithelial cells. Thus, the coexistence of two protein-tyrosine phosphatase activities in rat prostate, one associated with the acid phosphatase and the other related to SHP1, makes it necessary to analyze the importance of both activities in vivo and their possible function regarding prostatic cell growth and its regulation.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Valencia
authors.forename: J L
authors.initials: JL
authors.lastname: Oliva
authors.forename: G
authors.initials: G
authors.lastname: Bodega
authors.forename: A
authors.initials: A
authors.lastname: Chiloeches
authors.forename: P
authors.initials: P
authors.lastname: López-Ruiz
authors.forename: J C
authors.initials: JC
authors.lastname: Prieto
authors.forename: C
authors.initials: C
authors.lastname: Susini
authors.forename: B
authors.initials: B
authors.lastname: Colás
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D011467:Prostate / Q000201:enzymology*; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D000135:Acid Phosphatase; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases; C509250:Ptpn6 protein, rat