Pullulan production by Aureobasidium pullulans grown on ethanol stillage as a nitrogen source.
Pullulan production by Aureobasidium pullulans strain RP-1 using thin stillage from fuel ethanol production as a nitrogen source was studied in a medium using corn syrup as a carbon source. The use of 1% thin stillage as a nitrogen source instead of ammonium sulphate elevated polysaccharide production by strain RP-1 cells when grown on a concentration of up to 7.5% corn syrup, independent of yeast extract supplementation. Dry weights of cells grown in medium containing ammonium sulphate as the nitrogen source were higher than the stillage-grown cells after 7 days of growth. The viscosity of the polysaccharide on day 7 was higher for cells grown on thin stillage rather than ammonium sulphate as a nitrogen source. The pullulan content of the polysaccharide elaborated by ammonium sulphate-grown cells on day 7 was higher than the pullulan content of polysaccharide produced by stillage-grown cells regardless of whether yeast extract was added to the culture medium.
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Olson Biochemistry Laboratories, Department of Chemistry and Biochemistry, South Dakota State University, Brookings 57007, USA.
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D000645:Ammonium Sulfate / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005936:Glucans / Q000032:analysis / Q000096:biosynthesis*; D003904:Mitosporic Fungi / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D011134:Polysaccharides / Q000032:analysis; D003313:Zea mays / Q000378:metabolism
D003470:Culture Media; D005936:Glucans; D011134:Polysaccharides; D000431:Ethanol; C009109:pullulan; D009584:Nitrogen; D000645:Ammonium Sulfate