title: Two-step hydrolysis of amygdalin in molds.
abstract: Mucor circinelloides LU M40 and Penicillium aurantiogriseum P 35, characterized by extracellular beta-glucosidase activity on cyanogenic glycosides, hydrolyse amygdalin by a two-step reaction mechanism being the first step of hydrolysis, from amygdalin to prunasin, very rapid (15 min) and the second one, from prunasin to mandelonitrile, much slower (120 min).
authors.forename: L
authors.initials: L
authors.lastname: Brimer
authors.forename: A R
authors.initials: AR
authors.lastname: Cicalini
authors.forename: F
authors.initials: F
authors.lastname: Federici
authors.forename: R M
authors.initials: RM
authors.lastname: Nout
authors.forename: M
authors.initials: M
authors.lastname: Petruccioli
authors.forename: V
authors.initials: V
authors.lastname: Pulci
mesh_terms: D000097:Acetonitriles / Q000032:analysis / Q000378:metabolism; D000678:Amygdalin / Q000032:analysis / Q000378:metabolism*; D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003470:Culture Media; D005658:Fungi / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D008956:Models, Chemical; D009089:Mucor / Q000254:growth & development / Q000378:metabolism; D009570:Nitriles / Q000032:analysis / Q000378:metabolism; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors
chemical_list: D000097:Acetonitriles; D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D009570:Nitriles; C019063:prunasin; D000678:Amygdalin; C045516:mandelonitrile; D006026:Glycoside Hydrolases