Production of tumor necrosis factor and nitric oxide by macrophages infected with live and dead mycobacteria and their suppression by an interleukin-10-secreting recombinant.
We have analyzed mycobacterium-induced cytokine secretion in the J774A.1 macrophage-like cell line. Tumor necrosis factor alpha (TNF-alpha) was preferentially induced by live organisms, both slow and rapid growing. Expression of interleukin-10 by a recombinant strain of Mycobacterium smegmatis caused reduced production of TNF-alpha and nitric oxide during the early stages of infection.
Marshall
B G
BG
Department of Respiratory Medicine, Imperial College School of Medicine at St. Mary's, London, United Kingdom.
Chambers
M A
MA
Wangoo
A
A
Shaw
R J
RJ
Young
D B
DB
D015153:Blotting, Western; D002478:Cells, Cultured; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000276:immunology*; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology*; D009161:Mycobacterium / Q000235:genetics*; D009164:Mycobacterium Infections / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D009163:Mycobacterium bovis / Q000254:growth & development / Q000276:immunology*; D009569:Nitric Oxide / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D015164:Vaccines, Inactivated / Q000276:immunology
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D015164:Vaccines, Inactivated; D016753:Interleukin-10; D009569:Nitric Oxide