A simple and reliable method for delivering small fluid volumes to the brain of a freely moving rat.
A stepper motor-powered micropump for making intracerebral microinjections in freely moving animals is described. The micropump is capable of delivering fluid volumes of up to 20 microl in steps of 17 nl The system does not require a fluid swivel, delivers injections rapidly, maintains long-term calibration accuracy and is not susceptible to blockage of the cannula.
Hesse
G W
GW
Northeastern University, Boston, MA 02115, USA.
Stellar
J R
JR
Chevrette
J
J
D000818:Animals; D001921:Brain / Q000502:physiology*; D007260:Infusion Pumps; D008124:Locomotion / Q000502:physiology; D008845:Microinjections / Q000295:instrumentation* / Q000379:methods*; D051381:Rats