Development of an acute and chronic ecotoxicity assay using lux-marked Rhizobium leguminosarum biovar trifolii.
A soil isolate of Rhizobium leguminosarum bv. trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminescence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied. The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.
Paton
G I
GI
Department of Plant and Soil Science, University of Aberdeen, UK. g.i.paton@aberdeen.ac.uk
Palmer
G
G
Burton
M
M
Rattray
E A
EA
McGrath
S P
SP
Glover
L A
LA
Killham
K
K
D015374:Biosensing Techniques; D004784:Environmental Monitoring / Q000379:methods; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D008163:Luminescent Measurements; D008670:Metals / Q000032:analysis / Q000633:toxicity; D016961:Rhizobium leguminosarum / Q000187:drug effects / Q000235:genetics*; D012989:Soil Pollutants / Q000032:analysis* / Q000633:toxicity
D008670:Metals; D012989:Soil Pollutants