title: Learning impairment and cholinergic deafferentation after cortical nerve growth factor deprivation.
abstract: Cholinergic basal forebrain (CBF ) neurons have been shown to respond in vivo to exogenous administration of NGF. Although neurotrophins and their receptors are widely expressed in the CNS, little data exist for the physiological significance of endogenous neurotrophin signaling in CNS neurons. To test directly whether cortically derived NGF is functionally required for the cholinergic functions mediated by the cerebral cortex, repeated injections of anti-NGF mAbs were locally applied into the insular cortex (IC) of rats. The biochemical results, using an in vivo microdialysis technique, showed a dramatic lack of extracellular release of acetylcholine after high potassium stimulation compared with controls. Furthermore, by using small injections of the neurotracer fluorogold, we found a corresponding disruption in the connectivity between the IC and the CBF. Behavioral experiments showed that the NGF antibodies applied into the IC produced a significant disruption on the acquisition of conditioned taste aversion and inhibitory avoidance learning. However, the same animals were able to recall the taste aversion when the conditioning trial was established before injections of NGF antibodies. Given these results, it seems that cortical cholinergic functions are actively dependent on locally derived NGF in the adult normal brain, and that the cholinergic activity from the CBF is not necessary for recalling aversive stimuli, but is necessary for the acquisition of aversively motivated conditionings.
authors.affiliation: Departamento de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Mexico D. F., Mexico.
authors.forename: H
authors.initials: H
authors.lastname: Gutiérrez
authors.forename: M I
authors.initials: MI
authors.lastname: Miranda
authors.forename: F
authors.initials: F
authors.lastname: Bermúdez-Rattoni
mesh_terms: D000367:Age Factors; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology*; D001667:Binding, Competitive / Q000276:immunology; D002799:Cholinergic Fibers / Q000187:drug effects / Q000502:physiology*; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology; D003714:Denervation; D005456:Fluorescent Dyes; D008297:Male; D008568:Memory / Q000502:physiology; D008845:Microinjections; D009414:Nerve Growth Factors / Q000032:analysis / Q000276:immunology / Q000494:pharmacology*; D016548:Prosencephalon / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D013266:Stilbamidines; D013649:Taste / Q000502:physiology; D013788:Thalamus / Q000737:chemistry / Q000166:cytology / Q000502:physiology
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D009414:Nerve Growth Factors; D013266:Stilbamidines