title: In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes.
abstract: To analyze the mechanisms for in vitro emergence of the syncytial variants of herpes simplex virus type 1 (HSV-1), several cell lines were infected with a mixture of equal amounts of two HSV-1 variants, one syncytial and the other non-syncytial, and changes in their relative abundance were monitored during passage. With a combination of two variants of the Miyama strain of HSV-1, the syncytial variant became dominant during passage in Vero, RK-13 and FL cells. On the other hand, the ratios of the two variants remained around 1:1 during the passage in HEp-2, MGC and HEL cells. In another set of variants of the SKO strain of HSV-1, the outcomes were different from those of the Miyama strain in the FL, MGC and HEp-2 cells. The ratios of the two variants remained around 1:1 during passage in FL cells, while the non-syncytial variant became dominant during passage in MGC and HEp-2 cells. In addition, we examined the effects of a complement and interferon-beta (IFN-beta) on the outcome of the selection. As a result, the complement slowed the selection of a syncytial variant, whereas IFN-beta facilitated it.
authors.affiliation: Department of Virology, Okayama University Medical School, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Padilla
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Tsukazaki
authors.forename: J
authors.initials: J
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: F
authors.initials: F
authors.lastname: Uno
authors.forename: Y
authors.initials: Y
authors.lastname: Arao
authors.forename: S
authors.initials: S
authors.lastname: Nii
mesh_terms: D002460:Cell Line; D003165:Complement System Proteins / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral / Q000235:genetics; D014644:Genetic Variation; D015726:Giant Cells; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000472:pathogenicity*; D016899:Interferon-beta / Q000494:pharmacology; D012641:Selection, Genetic; D012692:Serial Passage; D014759:Viral Envelope Proteins
chemical_list: D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D016899:Interferon-beta; D003165:Complement System Proteins