title: Preparation of competent cells for high-efficiency plasmid transformation of Escherichia coli.
authors.affiliation: Tsukuba Life Science Center, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakata
authors.forename: X
authors.initials: X
authors.lastname: Tang
authors.forename: K K
authors.initials: KK
authors.lastname: Yokoyama
mesh_terms: D001431:Bacteriological Techniques; D002122:Calcium Chloride; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D007202:Indicators and Reagents; D010957:Plasmids; D013753:Tetracycline Resistance / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D007202:Indicators and Reagents; D002122:Calcium Chloride