Release of gelatinase A during platelet activation mediates aggregation.
Blood platelets limit blood loss at sites of vascular injury by forming a mechanical plug. They are also involved in thrombosis, atherosclerosis, inflammation and metastasis. Platelet activation is essential for these physiological and pathological reactions and depends upon their adhesion to the vessel wall and attachment to each other in the aggregation process. The two known pathways of aggregation are mediated by the release of endoperoxides/thromboxane A2 and ADP which amplify platelet aggregation. Here we report the identification of a new pathway of aggregation which is mediated by the release of a metalloproteinase enzyme, gelatinase A.
Sawicki
G
G
Department of Pharmacology, University of Alberta, Edmonton, Canada.
Salas
E
E
Murat
J
J
Miszta-Lane
H
H
Radomski
M W
MW
D000577:Amides / Q000494:pharmacology; D001081:Apyrase / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D015153:Blotting, Western; D003094:Collagen / Q000494:pharmacology; D011464:Epoprostenol / Q000494:pharmacology; D018093:Gelatinases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D009569:Nitric Oxide / Q000494:pharmacology; D010618:Phenanthrolines / Q000494:pharmacology; D015539:Platelet Activation / Q000502:physiology*; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D019716:Tissue Inhibitor of Metalloproteinase-2; D014443:Tyrosine / Q000031:analogs & derivatives / Q000494:pharmacology
D000577:Amides; D010618:Phenanthrolines; D010975:Platelet Aggregation Inhibitors; D011480:Protease Inhibitors; D011506:Proteins; D011994:Recombinant Proteins; C065557:N-(2-isobutyl-3-(N'-hydroxycarbonylamido)propanoyl)-O-methyltyrosinemethylamide; D019716:Tissue Inhibitor of Metalloproteinase-2; D009569:Nitric Oxide; D014443:Tyrosine; D003094:Collagen; D011464:Epoprostenol; D013917:Thrombin; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D001081:Apyrase; D013755:Tetradecanoylphorbol Acetate; D001241:Aspirin