Cloning and characterization of human cDNAs encoding a protein with high homology to rat intestinal development protein OCI-5.
We constructed a lambda complementary DNA expression library from the mitoxantrone-resistant human gastric carcinoma cell line EPG85-257RNOV. The library was screened by differential hybridization (resistant cell line against non-resistant cell variant). By this procedure we found five independent cDNA clones representing one single gene that has much higher expression in the mitoxantrone-resistant cell line EPG85-257RNOV than in the non-resistant variant EPG85-257P. One of the cDNA clones (MXR7) contains a complete open reading frame (ORF) encoding a 580-amino acid polypeptide. Amino acid and nucleotide sequence analysis revealed that this gene codes the human variant of a rat intestinal development protein OCI-5.
Lage
H
H
Institute of Pathology, Charité, Humboldt University Berlin, Germany. lage@rz.charite.hu-berlin.de
Dietel
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D053673:Glypicans; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D051381:Rats; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; C105937:GPC3 protein, human; D053673:Glypicans; C105936:Gpc3 protein, rat; D008565:Membrane Proteins; D009363:Neoplasm Proteins