Determination of the sequence of the aralkyl acyl-CoA:amino acid N-acyltransferase from bovine liver mitochondria.
The aralkyl acyl-CoA:amino-acid N-acyltransferase was previously purified to homogeneity from bovine liver mitochondria. The N-terminal amino-acid sequence and sequences obtained by cyanogen bromide cleavage of the enzyme were used to design oligonucleotide probes that were used to screen a bovine liver cDNA library. Several clones were isolated and sequenced, and the sequence is given. The cDNA contains 126 bases of 5'-untranslated region and 188 bp of 3' untranslated region. The cDNA codes for an enzyme containing 295 amino-acid residues. The sequence gives a molecular weight for the enzyme of 39,229, which is larger than previously estimated. The amino-acid composition of the enzyme, based on this sequence, is in agreement with the previously obtained amino-acid analysis on the purified kidney enzyme.
Vessey
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Liver Study Unit, Veterans Affairs Medical Center, San Francisco, CA 94121, USA.
Lau
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D000123:Acetyltransferases / Q000737:chemistry; D000217:Acyltransferases / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D018076:DNA, Complementary / Q000032:analysis; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data
D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D000217:Acyltransferases; D000123:Acetyltransferases; C078176:aralkyl-CoA-glycine N-acyltransferase; C067287:arylacetyl-CoA N-acetyltransferase