title: Human chromosome-specific cDNA libraries: new tools for gene identification and genome annotation.
abstract: To date, only a small percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome 5-specific cDNA libraries have been constructed from five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome 5 genes and 138 are from new chromosome 5 genes (a frequency of 79.5%). Sequence complexity estimates indicate that each library contains -20% of the approximately 5000 genes that are believed to reside on chromosome 5. This study more than doubles the number of genes mapped to chromosome 5 and describes an important new tool for disease gene isolation.
authors.affiliation: Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-8591, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Del Mastro
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: A D
authors.initials: AD
authors.lastname: Simmons
authors.forename: T D
authors.initials: TD
authors.lastname: Gallardo
authors.forename: G A
authors.initials: GA
authors.lastname: Clines
authors.forename: J A
authors.initials: JA
authors.lastname: Ashley
authors.forename: C J
authors.initials: CJ
authors.lastname: Hilliard
authors.forename: J J
authors.initials: JJ
authors.lastname: Wasmuth
authors.forename: J D
authors.initials: JD
authors.lastname: McPherson
authors.forename: M
authors.initials: M
authors.lastname: Lovett
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D018076:DNA, Complementary; D015723:Gene Library; D015894:Genome, Human; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012313:RNA
chemical_list: D018076:DNA, Complementary; C034477:RNA primers; D012313:RNA