Neuronal and muscular inclusions in rats with hindlimb dysfunction after treating with difluorobenzhydrylpiperadine.
Rats showing an ataxic gait induced by 20 wk of treatment with 0, 30, or 60 mg/kg of difluorobenzhydrylpiperadine (DFBP), a detriazinyl metabolic of almitrine, were examined by light microscopy and transmission electron microscopy. Vacuolar degeneration associated with lamellar inclusions was observed in musculus soleus and m. interossei of the hindlimbs in DFBP-treated rats. The inclusions were also produced within sensory neurons, satellite and Schwann cells, and vascular endothelial cells of thoracic and lumbar dorsal root ganglia as well as muscle spindles of affected muscles. Membrane-bound vacuoles containing electron-dense granules were seen in the peripheral nerves. This study demonstrated neuronal and muscular toxicity of DFBP in rats.
Yamanaka
Y
Y
Institute for Biomedical Research, Teijin Ltd., Hino, Japan.
Shimada
T
T
Mochizuki
R
R
Suzuki
Y
Y
Takenouchi
K
K
Takeda
T
T
Uno
H
H
Izawa
Y
Y
Fujiwara
K
K
D015765:Almitrine / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000633:toxicity*; D000818:Animals; D001259:Ataxia / Q000139:chemically induced*; D006614:Hindlimb / Q000503:physiopathology*; D002479:Inclusion Bodies / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D010525:Peripheral Nerves / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344
C106430:difluorobenzhydrylpiperazine; D015765:Almitrine