Inadequate function of sterile tw5/tw32 spermatozoa overcome by intracytoplasmic sperm injection.
Mice carrying two t complementary haplotypes (tw5/tw32) are totally sterile. Their spermatozoa have poor motility and fertilize neither zona-intact nor zona-free oocytes, even though they are structurally indistinguishable from control (wild-type) spermatozoa. However, when injected directly into oocytes, these infertile spermatozoa are able to participate in normal development. This suggests that infertility of tw5/tw32 male (spermatozoa) is more likely to be due to poor sperm-oocyte interaction than to genetic incompetence of sperm nuclei.
Kuretake
S
S
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822, USA.
Maleszewski
M
M
Tokumasu
A
A
Fujimoto
H
H
Yanagimachi
R
R
D000818:Animals; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D007248:Infertility, Male; D008297:Male; D051379:Mice; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*