Gas chromatography-mass spectrometry of Alternaria mycotoxins.
Heptafluorobutyrate (HFB) derivatives have not previously been used for GC of Alternaria mycotoxins. Capillary (0.5 micron film) GC-mass spectrometry (MS) showed that full and partial derivatives of alternariol (AOH), alternariol monomethyl ether (AME) and altenuene (ALT); a structurally uncharacterized derivative of altertoxin I (ALTX-I); and a tris-HFB derivative of tenuazonic acid (TA) were formed with heptafluorobutyric anhydride and a basic catalyst. Full and partial trimethylsilyl (TMS) ethers of these mycotoxins were formed with Tri-Sil TBT. Apple juice extracts caused increased response in GC-MS of AOH bis-HFB and bis-TMS derivatives. Natural occurrence of AOH in apple juice has been demonstrated.
Scott
P M
PM
Health Canada, Health Protection Branch, Food Research Division, Ottawa, Ont,, Canada.
Weber
D
D
Kanhere
S R
SR
D000528:Alternaria / Q000737:chemistry*; D001551:Benz(a)Anthracenes / Q000032:analysis; D001628:Beverages / Q000032:analysis; D002800:Cholinesterase Inhibitors / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005466:Fluorocarbons; D005638:Fruit; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007783:Lactones / Q000032:analysis; D009183:Mycotoxins / Q000032:analysis*; D010569:Perylene / Q000031:analogs & derivatives
D001551:Benz(a)Anthracenes; D002800:Cholinesterase Inhibitors; D005466:Fluorocarbons; D007783:Lactones; D009183:Mycotoxins; C040005:altenuene; C033094:perfluorobutyric acid; C050233:altertoxin I; D010569:Perylene; C005197:alternariol