Octopamine in the developing nervous system of the pond snail, Lymnaea stagnalis L.
Development of the octopaminergic system in the pond snail, Lymnaea stagnalis, was investigated by means of immunocytochemistry and radioenzymatic assay. The earliest octopamine-immunoreactive neurons appear at a late embryonic stage (E85) of development following metamorphosis. At this moment of development, the adult-like pattern of distribution and projection (arborization) characteristics of octopamine-immunoreactive neurone can already be observed. During hatching and postembryonic (juvenile) development the number of labelled neurons increases only within the ventro-medial cell groups of the cerebral ganglia, whereas the extent of varicose axon arborization of the labelled neurons increases gradually. No peripheral projections of the embryonic and postembryonic octopamine-immunoreactive neurons were observed. The postembryonic increase in number of immunoreactive neurons and development of axonal arborization is accompanied by a rapid, exponential enhancement of octopamine content of Lymnaea CNS, as detected radioenzymatically. A possible role of the octopaminergic neurons in the regulation of certain physiological function(s), active only from a late stage of embryonic development, is suggested.
Elekes
K
K
Department of Experimental Zoology, Balaton Limnological Research Institute of the Hungarian Academy of Sciences, Tihany, Hungary.
Voronezhskaya
E E
EE
Hiripi
L
L
Eckert
M
M
Rapus
J
J
D000818:Animals; D002490:Central Nervous System / Q000254:growth & development* / Q000378:metabolism*; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D008195:Lymnaea / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism; D009655:Octopamine / Q000378:metabolism*
D018377:Neurotransmitter Agents; D009655:Octopamine