Rapid and sensitive immunomagnetic separation-polymerase chain reaction method for the detection of Escherichia coli O157:H7 in raw milk and ice-cream.
Escherichia coli O157:H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157:H7/g food could be detected in < 10 h.
Gooding
C M
CM
CIFAR, Antibody Engineering Laboratory, University of California, Davis 95616-8584, USA.
Choudary
P V
PV
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D007054:Ice Cream / Q000382:microbiology*; D018189:Immunomagnetic Separation; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D022622:Shiga Toxin 1