Enhanced RACE method using specific enrichment by biotinylated oligonucleotides bound to streptavidin coated magnetic particles.
RACE (rapid amplification of cDNA ends) is commonly used for identification and isolation of 3'and 5'termini of cDNA. We developed an improvement of the RACE-method that allows the enrichment of wanted fragments. The important new feature is the purification of the amplified products by biotinylated oligonucleotides that hybridize internally. Hybrids are isolated by streptavidin coated magnetic particles.
Lankiewicz
S
S
Lehrstuhl für Zellphysiologie, Ruhr-Universität Bochum, Universitätsstrasse 150, D-44780 Bochum, Germany.
Gisselmann
G
G
Hatt
H
H
D001483:Base Sequence; D001710:Biotin; D003106:Colon; D017931:DNA Primers / Q000138:chemical synthesis* / Q000737:chemistry; D018076:DNA, Complementary; D015723:Gene Library; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000096:biosynthesis*; D044406:Receptors, Serotonin, 5-HT3
D017931:DNA Primers; D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D044406:Receptors, Serotonin, 5-HT3; D001710:Biotin