Molecular cloning of human GRB-7 co-amplified with CAB1 and c-ERBB-2 in primary gastric cancer.
Many amplified-chromosome regions in human cancers have been identified, while there are only a limited number of reports for amplified genes in a single amplicon. We recently isolated four cDNA clones, A39, B47, C51, and CAB1, from 500 kilobases of yeast artificial chromosome DNA containing the c-ERBB-2 gene. B47 consisted of 615 base pairs and had about 85% homology with the corresponding sequence of mouse Grb-7. We report here the structure of human GRB-7 cDNA, and we report that the amounts of mRNA for c-ERBB-2, CAB1, and GRB-7 were elevated in concordance with the amplification.
Kishi
T
T
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Sasaki
H
H
Akiyama
N
N
Ishizuka
T
T
Sakamoto
H
H
Aizawa
S
S
Sugimura
T
T
Terada
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D051382:GRB7 Adaptor Protein; D005784:Gene Amplification; D018734:Genes, erbB-2; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; C495955:GRB7 protein, human; C495956:Grb7 protein, mouse; D011506:Proteins; D012333:RNA, Messenger; D051382:GRB7 Adaptor Protein