title: Respiratory tract cryptosporidiosis in immunosuppressed rat is associated with an epithelial metaplasia.
abstract: Cryptosporidium parvum is an opportunistic protozoa that chronically infects the digestive tract of immunocompromised hosts. Respiratory cryptosporidiosis, which was reported in AIDS patients, is an uncommon feature of mammalian cryptosporidiosis models. In this study, we document the respiratory lesion; observed in an immunosuppressed rat model of cryptosporidiosis. Twenty rats were immunosuppressed with corticosteroids and low protein diet. They were challenged intratracheally with 10(6) C. parvum sporozoites. Lungs and ileums were examined on D3, D6, D10, D14. On D10 and D14, C. parvum were present in the respiratory tract of all animals in association with the progressive appearance of an immature malpighian metaplasia. On D14, an intestinal infection was also detected in 2/4 animals. The respiratory tract appears to be a fully permissive area for the protozoa in immunosuppressed rats. Introduction of parasites on the respiratory mucosa seems a requisite to induce respiratory cryptosporidiosis. This experimental protocol yields a low mortality rate, and so modelizes late and/or chronic stages of respiratory cryptosporidiosis.
authors.affiliation: Laboratoires d'Histologie, Groupe de recherches ERPUR, CHU de Rouen.
authors.forename: F
authors.initials: F
authors.lastname: Roussel
authors.forename: D
authors.initials: D
authors.lastname: Lemeteil
authors.forename: L
authors.initials: L
authors.lastname: Favennec
authors.forename: J
authors.initials: J
authors.lastname: Tayot
authors.forename: J J
authors.initials: JJ
authors.lastname: Ballet
authors.forename: P
authors.initials: P
authors.lastname: Brasseur
mesh_terms: D000818:Animals; D001980:Bronchi / Q000469:parasitology / Q000473:pathology*; D002923:Cilia / Q000469:parasitology / Q000473:pathology; D003457:Cryptosporidiosis / Q000276:immunology / Q000473:pathology*; D016785:Cryptosporidium parvum / Q000302:isolation & purification / Q000502:physiology*; D004195:Disease Models, Animal; D004848:Epithelium / Q000469:parasitology / Q000473:pathology; D006801:Humans; D007082:Ileum / Q000469:parasitology / Q000473:pathology; D016867:Immunocompromised Host; D007165:Immunosuppression Therapy; D008174:Lung Diseases, Parasitic / Q000276:immunology / Q000473:pathology*; D008679:Metaplasia; D051381:Rats; D017207:Rats, Sprague-Dawley; D014132:Trachea / Q000469:parasitology / Q000473:pathology*