title: Frameshift intermediates in homopolymer runs are removed efficiently by yeast mismatch repair proteins.
abstract: A change in the number of base pairs within a coding sequence can result in a frameshift mutation, which almost invariably eliminates the function of the encoded protein. A frameshift reversion assay with Saccharomyces cerevisiae that can be used to examine the types of insertions and deletions that are generated during DNA replication, as well as the editing functions that remove such replication errors, has been developed. Reversion spectra have been obtained in a wild-type strain and in strains defective for defined components of the postreplicative mismatch repair system (msh2, msh3, msh6, msh3 msh6, pms1, and mih1 mutants). Comparison of the spectra reveals that yeast mismatch repair proteins preferentially remove frameshift intermediates that arise in homopolymer tracts and indicates that some of the proteins have distinct substrate or context specificities.
authors.affiliation: Graduate Program in Genetics and Molecular Biology, Emory University, Atlanta, Georgia 30322, USA.
authors.forename: C N
authors.initials: CN
authors.lastname: Greene
authors.forename: S
authors.initials: S
authors.lastname: Jinks-Robertson
mesh_terms: D000251:Adenosine Triphosphatases; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins; D004260:DNA Repair; D004261:DNA Replication; D004271:DNA, Fungal / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D029968:Escherichia coli Proteins; D016368:Frameshift Mutation; D005656:Fungal Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein; D000074081:MutS Homolog 3 Protein; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; C081696:MSH3 protein, S cerevisiae; C098790:MSH6 protein, S cerevisiae; C074770:MutL protein, E coli; D000074081:MutS Homolog 3 Protein; D009692:Nucleic Acid Heteroduplexes; C088858:PMS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; C489751:MSH2 protein, S cerevisiae; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein