Retinoblastoma protein expressed in human non-Hodgkin's lymphoma cells generates resistance against radiation-induced apoptosis.
Inactivation of the retinoblastoma susceptibility (Rb) gene has been observed in various cancers. Nevertheless, in several cancer cases, including non-Hodgkin's lymphoma, previous investigations showed that the Rb gene product (pRb) was abundantly expressed with varying degree. Here we report the THS-SP1.1 cell line isolated from non-Hodgkin's lymphoma. The THS-SP1.1 cells abundantly expressed pRb and showed resistance against radiation-induced apoptosis. Culture with the antisense phosphorothioate oligonucleotide complementary to the Rb gene augmented the apoptosis of THS-SP1.1 cells after radiation, whereas the control oligonucleotides did not. These data showed that pRb abundantly expressed in the lymphoma cells inhibited radiation-induced apoptosis.
Ishii
H
H
Second Department of Internal Medicine, Chiba University, Japan.
Igarashi
T
T
Saito
T
T
Nakano
T
T
Mori
M
M
Ohyama
H
H
Miyamoto
T
T
Saito
Y
Y
Oh
H
H
D017209:Apoptosis / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology / Q000532:radiotherapy*; D011839:Radiation, Ionizing; D016160:Retinoblastoma Protein / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D016160:Retinoblastoma Protein