Role of Rho GTPase in the endothelin-1-induced nuclear signaling.
Binding of Endothelin-1 (ET-1) to its heterotrimeric G protein-coupled receptors stimulates various signaling cascades involving the activation of phospholipase C-beta, phospholipase D, protein kinase C (PKC), tyrosine kinases, Ca2+/calmodulin-dependent kinase (CaMKs), and Ras, a small molecular weight G-protein, but, the role of Rho GTPase remains unclear. In this project, we examined whether RhoA contributes to the ET-1-induced signaling cascade to c-fos SRE activation in Rat-2 fibroblast cells. Our results demonstrate that Rho activation is critical for the signal transduction of ET-1 to c-fos SRE.
Kim
J H
JH
Laboratory of Molecular & Cellular Genetics, Hallym University, Chun-Cheon, Korea. jhkim@sun.hallym.ac.kr
Cho
Y S
YS
Kim
B C
BC
Kim
Y S
YS
Lee
G S
GS
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004268:DNA-Binding Proteins; D019332:Endothelin-1 / Q000378:metabolism*; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D016762:Genes, fos; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1
D004268:DNA-Binding Proteins; C494114:Elk1 protein, rat; D019332:Endothelin-1; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1; D020558:GTP Phosphohydrolases