title: Lectin-binding glycoconjugates in the subfornical organ of the rat.
abstract: In the rat subfornical organ (SFO), lectin-binding glycoconjugates were histochemically examined by 9 biotinylated lectins using a streptavidin-biotin peroxidase system. The strong or moderate binding of Canavalia ensiformis (Con A), Lens culinaris (LCA) and Phaseolus vulgaris erythroagglutinin (ePHA) indicated overall distributions of high mannose, intermediate, hybrid N-linked, and non-bisected, bi/triantennate N-linked complex oligosaccharides in the rat SFO. Nonsialylated terminal N-acetylglucosamines were detected throughout this organ, as revealed by its stainabilities with Triticum vulgaris (WGA) and Limax flavus (LFA). In this organ, Ricinus communis (RCA-1) specifically bound to vessel-associated structures, whereas Arachis hypogaea (PNA) reacted with selected neurons in the central and rostral regions of this organ. Dolichos biflorus (DBA) and Ulex europaeus (UEA-1) did not stain any histologic structures in the rat SFO. The results obtained in this study provide a basis for comprehensive analyses of glycoconjugates in the rat SFO.
authors.affiliation: 2nd Department of Anatomy, Nagoya City University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ueda
authors.forename: O
authors.initials: O
authors.lastname: Fujimori
authors.forename: K
authors.initials: K
authors.lastname: Yamada
mesh_terms: D000818:Animals; D006001:Glycoconjugates / Q000032:analysis / Q000378:metabolism*; D006651:Histocytochemistry; D037102:Lectins / Q000032:analysis / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013356:Subfornical Organ / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
chemical_list: D006001:Glycoconjugates; D037102:Lectins