An immunohistochemical screening of neurochemical markers in fungiform papillae and taste buds of the anterior rat tongue.
The occurrence and distribution of several neurochemical markers were investigated. Numerous nerve fibres were shown, using antibodies to protein gene product (PGP) 9.5, neurone-specific enolase, calcitonin gene-related peptide (CGRP), substance P. neurokinin A or protein S-100. The presence of vasoactive intestinal polypeptide (VIP), peptide histidine isoleucine amide (PHI), neuropeptide tyrosine, dopamine-beta-hydroxylase (DBH), cholecystokinin/gastrin, glutamate and galanin was more scarce. Nerve fibres containing these above-mentioned markers were found at several locations, i.e. in the epithelium, connective tissue, and around blood vessels. In the taste buds, numerous PGP 9.5, neurone-specific enolase-, CGRP-, substance P-, neurokinin A- and protein S-100-containing structures were found, but few VIP and galanin ones. No immunoreactivity was found with antibodies against somatostatin, bombesin, enkephalin or dynorphin. These findings extend knowledge about the general as well as the neurochemical messenger-based innervation of rat fungiform papillae, forming a firm basis for future functional investigations of normal, experimental and also clinical materials.
Astbäck
J
J
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Arvidson
K
K
Johansson
O
O
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002766:Cholecystokinin / Q000032:analysis; D004299:Dopamine beta-Hydroxylase / Q000032:analysis; D019084:Fluorescent Antibody Technique, Indirect; D019004:Galanin / Q000032:analysis; D005755:Gastrins / Q000032:analysis; D018698:Glutamic Acid / Q000032:analysis; D009412:Nerve Fibers / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000032:analysis*; D015288:Neurokinin A / Q000032:analysis; D009478:Neuropeptide Y / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D010451:Peptide PHI / Q000032:analysis; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000032:analysis; D013373:Substance P / Q000032:analysis; D013650:Taste Buds / Q000737:chemistry*; D013869:Thiolester Hydrolases / Q000032:analysis; D014059:Tongue / Q000737:chemistry / Q000294:innervation*; D043222:Ubiquitin Thiolesterase; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D005755:Gastrins; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D009479:Neuropeptides; D010451:Peptide PHI; D009418:S100 Proteins; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D018698:Glutamic Acid; D015288:Neurokinin A; D019004:Galanin; D002766:Cholecystokinin; D004299:Dopamine beta-Hydroxylase; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase; D010751:Phosphopyruvate Hydratase; D015740:Calcitonin Gene-Related Peptide