Effects of androgen receptor polyglutamine tract expansion on proliferation of NG108-15 cells.
Expansion of the polyglutamine tracts in the androgen receptor (AR) has been recognized as a cause of X-linked spinal and bulbar muscular atrophy (SBMA). In the present study, NG108-15 cells were stably transfected with expression vectors coding for either the wild type (WT) AR gene (CAG repeat number = 22) or a mutated (MT) AR gene (CAG repeat number = 52). Cells proliferation and cell cycle parameters were evaluated for NG108-15-WT and NG108-15-MT cells in the presence or absence of androgen. NG108-15-WT cells demonstrated an androgen-dependent increase in cell number, while NG108-15-MT cells did not. Our results demonstrate that expansion of polyglutamine tracts in the AR may affect the proliferation and differentiation of nerve cells.
Nakajima
H
H
First Department of Internal Medicine, Osaka Medical College, Takatsuki City, Japan.
Kimura
F
F
Nakagawa
T
T
Ikemoto
T
T
Furutama
D
D
Shinoda
K
K
Kato
S
S
Shimizu
A
A
Ohsawa
N
N
D000728:Androgens / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology; D005910:Glioma; D006822:Hybrid Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D051379:Mice; D016296:Mutagenesis / Q000502:physiology; D009447:Neuroblastoma; D010455:Peptides / Q000378:metabolism*; D010957:Plasmids; D011944:Receptors, Androgen / Q000032:analysis / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000187:drug effects
D000728:Androgens; D010455:Peptides; D011944:Receptors, Androgen; C097188:polyglutamine