BCR ligation induces receptor editing in IgM+IgD- bone marrow B cells in vitro.
The ability of BCR cross-linking to stimulate receptor editing was analyzed in vitro using bone marrow B cells from immunoglobulin (Ig) transgenic (Tg) and non-Tg mice. In cultured Ig-Tg cells, BCR ligation induced receptor editing as measured by up-regulation of RAG gene expression, light chain gene DNA rearrangements, and expression of lambda-light chain protein in cells that previously expressed kappa. In the culture conditions used, BCR ligation induced light chain rearrangements in most immature IgM+IgD- bone marrow B cells in the absence of significant cell death or cell growth. Receptor editing in non-Tg B cells was also documented in cultures treated with anti-immunoglobulin. These results provide direct evidence for the ability of BCR ligation to stimulate immunoglobulin light chain gene rearrangements in immature B cells.
Hertz
M
M
Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA.
Nemazee
D
D
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells; D016923:Cell Death / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002460:Cell Line; D005260:Female; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain / Q000276:immunology; D007072:Immunoglobulin D / Q000378:metabolism*; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000235:genetics; D007075:Immunoglobulin M / Q000378:metabolism*; D007146:Immunoglobulin lambda-Chains / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism* / Q000502:physiology
D007072:Immunoglobulin D; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007146:Immunoglobulin lambda-Chains; D011947:Receptors, Antigen, B-Cell