Arabinofuranosyl-terminated and mannosylated lipoarabinomannans from Mycobacterium tuberculosis induce different levels of interleukin-12 expression in murine macrophages.
Lipoarabinomannan (LAM) is a major surface lipoglycan of Mycobacterium tuberculosis. In the present study, we demonstrated that arabinofuranosyl-terminated LAM (AraLAM) derived from a rapidly growing Mycobacterium sp., but not extensively mannosylated LAM derived from the Erdman strain, is capable of inducing interleukin-12 (IL-12) expression in murine macrophages. Since IL-12 is known to drive the differentiation of naive T cells toward T-helper type 1 (Th1) cell development, AraLAM may be an effective adjuvant in vaccines and immunotherapies that need Th1 responses.
Yoshida
A
A
Department of Microbiology and Immunology, School of Medicine, Hamamatsu University, Handa-cho, Japan. yoshida@hama-med.ac.jp
Koide
Y
Y
D000818:Animals; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
D008070:Lipopolysaccharides; D012333:RNA, Messenger; C050016:lipoarabinomannan; D018664:Interleukin-12