title: Mechanical forces alter growth factor release by pleural mesothelial cells.
abstract: The mesothelial cells that form the visceral pleura of the lung are subjected to physical forces such as stretch due to lung expansion and fluid shear stress due to the sliding motion of the lung against the chest wall. In this study, the effect of mechanical forces on the production of growth factors by mesothelial cells was investigated. Rat visceral pleura mesothelial (RVPM) cells were exposed to fluid shear stress by perfusing a column of cell-covered beads. RVPM cells grown on a silicone elastomer were subjected to cyclic strain by applying an oscillating vacuum to the bottom of the wells using the Flexercell apparatus. Fluid shear stress (5.2-15.7 dyn/cm2) stimulated the release of endothelin-1 (ET-1) by RVPM cells two- to fivefold over static cells. ET-1 secretion by RVPM cells was also stimulated approximately twofold by cyclic stretch (20% maximum strain, 30 cycles/min). RVPM cells released significant levels of platelet-derived growth factor (PDGF), but there was no effect of either shear stress or cyclic strain on PDGF release. These results suggest that the production of growth factors by pleural mesothelial cells is regulated in part by physical forces.
authors.affiliation: Department of Anesthesia, Northwestern University, Chicago, Illinois 60611, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Waters
authors.forename: J Y
authors.initials: JY
authors.lastname: Chang
authors.forename: M R
authors.initials: MR
authors.lastname: Glucksberg
authors.forename: N
authors.initials: N
authors.lastname: DePaola
authors.forename: J B
authors.initials: JB
authors.lastname: Grotberg
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D012212:Rheology; D013314:Stress, Mechanical
chemical_list: D019332:Endothelin-1; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor