title: mle-1, a mariner-like transposable element in the nematode Trichostrongylus colubriformis.
abstract: A mariner-like element termed mle-1 was discovered in the parasitic nematode Trichostrongylus colubriformis. The mle-1 has features which support its assignment as a mariner-like transposable element. Cloned mle-1 was derived from an intron of the tar-1 gene. It comprises 893 bp, includes two 27 bp flanking perfect inverted repeats and is present at approximately 50 copies in the genome. The element contains a coding region which displays homology to transposases, with the greatest amino acid similarity to a Caenorhabditis elegans mariner-like transposase. The coding region contains two 12 bp repeats; these repeats flank an 11 bp segment which accounts for a frameshift in this region. As a candidate transposon, mle-1 provides potential for genetic manipulation of this and related organisms.
authors.affiliation: Department of Veterinary Pathology, University of Sydney, NSW, Australia.
authors.forename: L J
authors.initials: LJ
authors.lastname: Wiley
authors.forename: L G
authors.initials: LG
authors.lastname: Riley
authors.forename: N C
authors.initials: NC
authors.lastname: Sangster
authors.forename: A S
authors.initials: AS
authors.lastname: Weiss
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004251:DNA Transposable Elements / Q000235:genetics; D018509:DNA, Helminth; D015801:Helminth Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D014254:Trichostrongylus / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D018509:DNA, Helminth; D015801:Helminth Proteins