Reversibility of monocytic differentiation of HL-60 cells by 1alpha,25 dihydroxyvitamin D3.
Although 1alpha,25(OH)2D3 induces HL-60 cells to differentiate into monocytes, whether or not these monocytes revert to native promyelocytes is not clear. To investigate this question, HL-60 cells were treated with 1alpha,25(OH)2D3, and divided into CD14-positive and -negative cells with a cell sorter. These two populations were cultured with or without 1alpha,25(OH)2D3. Sorted CD14-positive HL-60 cells, treated with 1alpha,25(OH)2D3 for 7 days, reverted to CD14-negative cells and promyelocyte-like cells if 1alpha,25(OH)2D3 was removed from the medium. We conclude that the 1alpha,25(OH)2D3-induced differentiation of HL-60 cells into monocytes is reversible, and continuous administration of 1alpha,25(OH)2D3 is required for the differentiation of HL-60 cells.
Tomoyasu
S
S
Department of Hematology, Showa University School of Medicine, Shinagawa-ku, Tokyo, Japan.
Fukuchi
K
K
Watanabe
K
K
Ueno
H
H
Hamano
Y
Y
Hisatake
J
J
Hino
K
K
Gomi
K
K
Tsuruoka
N
N
D002117:Calcitriol / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D016130:Immunophenotyping; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D002117:Calcitriol