Wortmannin enhances CPP32-like activity during neuronal differentiation of P19 embryonal carcinoma cells induced by retinoic acid.
P19 EC cells undergoes apoptosis during neuronal differentiation induced by retinoic acid. Two CPP32-like proteases, CPP32 and Mch-3, are expressed in untreated and retinoic acid-treated P19 EC cells. CPP32-like activity is remarkably increased in apoptosis during neuronal differentiation of P19 EC cells. Inhibition of CPP32-like proteases prevents apoptosis, suggesting that activation of CPP32-like proteases play central roles in the apoptosis during neuronal differentiation of P19 EC cells. Wortmannin, PI-3K inhibitor, enhances the CPP32-like activity of the retinoic acid-treated P19 EC cells. PI-3K may be involved in the apoptosis during neuronal differentiation as negative regulator.
Mukasa
T
T
Division of Development and Differentiation, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Khoroku
Y
Y
Tsukahara
T
T
Momoi
M Y
MY
Kimura
I
I
Momoi
T
T
D000730:Androstadienes / Q000494:pharmacology*; D017209:Apoptosis; D018236:Carcinoma, Embryonal / Q000473:pathology; D053148:Caspase 3; D020169:Caspases; D002454:Cell Differentiation / Q000187:drug effects*; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D053938:DNA Fragmentation; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D000077191:Wortmannin
D000730:Androstadienes; D014212:Tretinoin; D053148:Caspase 3; D020169:Caspases; D003546:Cysteine Endopeptidases; D000077191:Wortmannin