The effect of intrathecal MBP synthetic peptides containing epitope P85 VVHFFKNIVTP96 on free anti-MBP levels in acute relapsing multiple sclerosis.
Acute relapses of multiple sclerosis (MS) are characterized by elevated Free (F)/Bound (B) anti-MBP ratios during the initial phase, followed by a steady decline of F antibody as the recovery/remission phase develops. The (human) MBP epitope for MS anti-MBP is: Pro85-Val-Val-His-Phe-Phe-Lys-Asn-Ile-Val-Thr-Pro96. In phase one clinical research, synthetic peptides (p) containing this epitope, namely pMBP86-95 and/or pMBP82-98, were intrathecally administered to MS patients with monosymptomatic or polysymptomatic relapses to determine the dosage, frequency and duration of administration which will immediately neutralize F circulating CSF anti-MBP. Patients with monosymptomatic relapses required 50 mg of peptide administered daily for 4-5 days. In patients with polysymptomatic relapses, F anti-MBP can be neutralized with dosages between 50 mg peptide daily for 4 days up to 100 mg twice a day for 2 days; however due to the prolonged nature of polysymptomatic relapses, antibody neutralization could not be maintained by these short courses of intrathecal peptide administration. Intravenous administration of these same peptides did not prevent occurrence of future relapses.
Warren
K G
KG
Department of Medicine (Neurology), University of Alberta, Edmonton, Canada.
Catz
I
I
D000208:Acute Disease; D000328:Adult; D001323:Autoantibodies / Q000276:immunology; D000939:Epitopes / Q000008:administration & dosage* / Q000134:cerebrospinal fluid / Q000276:immunology; D006801:Humans; D007275:Injections, Intravenous; D007278:Injections, Spinal; D008297:Male; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology; D004676:Myelin Basic Protein / Q000008:administration & dosage* / Q000134:cerebrospinal fluid / Q000138:chemical synthesis; D010455:Peptides / Q000008:administration & dosage* / Q000134:cerebrospinal fluid / Q000138:chemical synthesis; D011485:Protein Binding / Q000502:physiology; D012008:Recurrence
D001323:Autoantibodies; D000939:Epitopes; D004676:Myelin Basic Protein; D010455:Peptides