In vitro photo-activation of newly synthesized chlorin derivatives with red-light-emitting diodes.
We investigated the in vitro photo-activation properties of two chlorin derivatives, i.e. 8-cis-heptylchlorin dicarboxylic acid and 3-trans-heptylchlorin bisamidoglucose derivative, which exhibit lipophilic properties similar to those of the active fractions of Photofrin II, on a normal epithelial cell line (FRTL-5). We used as an irradiation source an array of diodes emitting red light (lambda = 675 nm), which produced a fluence of 7mW cm-2 on the cells. We found that photo-activation with chlorin derivatives in the concentration range 1-100 ng ml-1 greatly enhanced the mortality of the irradiated cells (energy density, 0.25 J cm-2) with respect to the control cells kept in the dark. This response is immediate and appears to be an "all or none' effect. Taking into account that compounds exhibit a strong absorbance peak in the long wavelength region of visible light where tissues are relatively transparent, our results suggest that chlorins can be considered to be good candidates for application in photodynamic therapy.
Colasanti
A
A
Dipartimento di Biologia e Patologia Cellulare e Moleculare, University of Naples Federico II, Italy.
Kisslinger
A
A
Kusch
D
D
Liuzzi
R
R
Mastrocinque
M
M
Montforts
F P
FP
Quarto
M
M
Riccio
P
P
Roberti
G
G
Villani
F
F
D000818:Animals; D008027:Light; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D013054:Spectrophotometry, Atomic; D013961:Thyroid Gland / Q000187:drug effects / Q000528:radiation effects
C106122:2-heptylchlorin dicarboxylic acid; C106123:3-heptylchlorin bisamidoglucose; D017319:Photosensitizing Agents; D011166:Porphyrins