title: Effect of one-HLA-haplotype-matched and HLA-mismatched blood transfusions on recipient T lymphocyte allorepertoires.
abstract: BACKGROUND
  Pretransplant blood transfusion has a well-known beneficial effect on posttransplant graft survival. Recently, it has been proposed that the clinical benefit of transfusion is due to HLA-DR antigen sharing between the blood donor(s) and the recipient. Immunological studies have suggested that this might result from a functional deletion of donor-reactive cytotoxic T lymphocytes.
  
  
  METHODS
  We investigated frequencies of alloreactive lymphocyte precursors with cytotoxic or interleukin-2-producing helper function by limiting dilution analysis in 10 renal dialysis patients before and after transfusion with fresh, allogeneic whole blood. Five patients received blood transfusions from donors matched for one HLA haplotype (or one HLA-B-DR antigen) and the other five patients received blood from fully HLA-mismatched donors.
  
  
  RESULTS
  Contrary to some previous reports, frequency analysis of cytotoxic T lymphocyte precursors revealed no significant differences between the two treatment groups in terms of development of blood donor-specific hyporesponsiveness after transfusion. Split-well analysis of cytotoxic T lymphocyte precursors reactive with single-mismatched HLA antigens demonstrated that the effects of transfusion on alloreactive specificity are complex and may vary depending on the particular antigens mismatched between the recipient and blood donor. Analysis of donor-specific helper T lymphocyte precursor frequencies revealed a significant decrease of interleukin-2-producing cells 3 months after transfusion in the total patient population. This effect was most prominent in the recipients of HLA-mismatched blood, but it also exhibited some degree of nonspecificity, as frequencies of third-party reactive helper T lymphocyte precursors were also significantly reduced.
  
  
  CONCLUSIONS
  Our overall results suggest that the degree of HLA matching between blood donor and recipient does not greatly influence the effect of blood transfusion on the T lymphocyte allorepertoire. The apparent induced down-regulation of helper T lymphocyte activity may play a role in the reported immunosuppressive effects of allogeneic blood transfusion.
authors.affiliation: Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Headington, England.
authors.forename: N T
authors.initials: NT
authors.lastname: Young
authors.forename: D L
authors.initials: DL
authors.lastname: Roelen
authors.forename: N
authors.initials: N
authors.lastname: Iggo
authors.forename: D W
authors.initials: DW
authors.lastname: Gray
authors.forename: J A
authors.initials: JA
authors.lastname: Roake
authors.forename: V
authors.initials: V
authors.lastname: Graham
authors.forename: K J
authors.initials: KJ
authors.lastname: Wood
authors.forename: M J
authors.initials: MJ
authors.lastname: Dallman
authors.forename: K I
authors.initials: KI
authors.lastname: Welsh
authors.forename: P J
authors.initials: PJ
authors.lastname: Morris
mesh_terms: D000328:Adult; D001782:Blood Donors; D001788:Blood Grouping and Crossmatching; D001803:Blood Transfusion; D002452:Cell Count; D006680:HLA Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D007519:Isoantigens / Q000032:analysis; D008297:Male; D008875:Middle Aged; D013234:Stem Cells / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology; D013997:Time Factors
chemical_list: D006680:HLA Antigens; D007519:Isoantigens