title: DNA strand exchange mediated by the Escherichia coli RecA protein initiates in the minor groove of double-stranded DNA.
abstract: The Escherichia coli RecA protein can recognize sequence homology between a single-stranded DNA (ssDNA) and homologous double-stranded DNA (dsDNA). One model for the homology recognition invokes a DNA triplex intermediate in which specific hydrogen bonds connect the ssDNA with groups in the major groove of dsDNA. Using photo-cross-linking methods, we have analyzed the arrangement of DNA strands after the local strand exchange. The results showed that the displaced strand sits in the major groove of the hybrid duplex product. This arrangement indicates that the ssDNA invades the minor groove of dsDNA and hence argues against the involvement of triplex intermediates. The results support an alternative model for the homology recognition that invokes melting of the dsDNA and annealing of the one strand to the invading ssDNA.
authors.affiliation: Rockefeller University, New York 10021, USA.
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: K
authors.initials: K
authors.lastname: Adzuma
mesh_terms: D001483:Base Sequence; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D010777:Photochemistry; D011938:Rec A Recombinases / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D011995:Recombination, Genetic; D013379:Substrate Specificity
chemical_list: D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D009692:Nucleic Acid Heteroduplexes; D011938:Rec A Recombinases