Optimization of RAPD for fingerprinting Salmonella.
Random amplification of polymorphic DNA (RAPD) is proving to be a useful technique in studying the epidemiology of micro-organisms. The technique can be troublesome and time consuming to establish due to the essentially empirical approach to optimization. By standardization of certain parameters and use of a commercially available PCR buffer optimization kit, a particularly promising primer was identified and RAPD conditions for a highly discriminatory and reproducible characterization of Salmonella isolates was achieved. In addition, a technique to obtain reproducible RAPD fingerprints of Salmonella isolates without the need to purify genomic DNA is described.
Hilton
A C
AC
School of Biological Sciences, University of Birmingham, UK. A.C.Hilton@bham.ac.uk
Banks
J G
JG
Penn
C W
CW
D000818:Animals; D001483:Base Sequence; D002021:Buffers; D016172:DNA Fingerprinting / Q000379:methods* / Q000706:statistics & numerical data; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D019105:Random Amplified Polymorphic DNA Technique / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification
D002021:Buffers; D017931:DNA Primers; D004269:DNA, Bacterial