title: Cytotoxic T lymphocyte changes after HLA-DR match and HLA-DR mismatch blood transfusions.
abstract: In the present study, we analyzed transfusion-induced cytolytic T lymphocyte (CTL) changes in patients who received either a one-HLA-DR-match or a zero-HLA-DR-match pretransplant blood transfusion. Twenty-four nonimmunized naive patients awaiting kidney transplantation received one planned, HLA-typed blood transfusion. The frequencies of CTL precursors (CTLp) directed against blood donor cells and controls were evaluated before and sequentially at days 7, 28, and 60 after transfusion. Results showed that sharing one HLA-DR between donor and recipient did not prevent CTL sensitization. Indeed, (1) An increase of donor-specific CTLp frequencies was observed in 8 of 11 patients who received a zero HLA-DR match (71%), as well as in 9 of 13 patients who received a one-HLA-DR-match (69%) transfusion. (2) This increase occurred with similar kinetics in the two groups, as it was highly significant 7 days after transfusion (P=0.002 and P=0.0035 in the first and second groups, respectively) but transient; CTLp frequencies returned to pretransfusion levels by day 60 after transfusion in both groups. (3) Finally, the magnitude of this increase was similar in the DR-match and DR-mismatch groups. Thus, if it is confirmed, in clinical practice, that one DR match between the blood donor and the patient would improve the tolerance effect of pretransplant blood transfusion, this phenomenon is unlikely to be related to the prevention of CTL sensitization.
authors.affiliation: Laboratoire d'Immunologie, Hopital Robert Debre, Paris, France. Group Coopératif de Transplantation d'Ile de France.
authors.forename: V
authors.initials: V
authors.lastname: Baudouin
authors.forename: N
authors.initials: N
authors.lastname: de Vitry
authors.forename: C
authors.initials: C
authors.lastname: Hiesse
authors.forename: P
authors.initials: P
authors.lastname: Lang
authors.forename: J
authors.initials: J
authors.lastname: Bloch
authors.forename: S
authors.initials: S
authors.lastname: Legouvello
authors.forename: G
authors.initials: G
authors.lastname: Sterkers
mesh_terms: D001782:Blood Donors; D001788:Blood Grouping and Crossmatching; D001803:Blood Transfusion; D017637:Clonal Deletion; D005260:Female; D015235:HLA-B Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000097:blood*; D006239:Haplotypes; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D007700:Kinetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013997:Time Factors
chemical_list: D015235:HLA-B Antigens; D006684:HLA-DR Antigens