title: Regulation by oestrogens of tachykinin NK3 receptor expression in the rat uterus.
abstract: The expression of the tachykinin NK3 receptor and its regulation by ovarian steroids were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) in uteri from ovariectomized rats. A single transcript corresponding to the 325-bp product expected for the tachykinin NK3 receptor was detected in uteri from olive oil-treated (control) ovariectomized rats. The level of tachykinin NK3 receptor mRNA in progesterone-treated animals was similar to that observed in uteri from control ones. Tachykinin NK3 receptor mRNA levels were significantly smaller in uteri from oestrogen-treated ovariectomized rats, with approximately a 32-fold decrease. These findings suggest that oestrogen, but not progesterone, regulates the expression of tachykinin NK3 receptors in the rat uterus.
authors.affiliation: Instituto de Investigaciones Científicas Isla de La Cartuja, Sevilla, Spain.
authors.forename: F M
authors.initials: FM
authors.lastname: Pinto
authors.forename: J
authors.initials: J
authors.lastname: Magraner
authors.forename: P
authors.initials: P
authors.lastname: Ausina
authors.forename: E
authors.initials: E
authors.lastname: Anselmi
authors.forename: J D
authors.initials: JD
authors.lastname: Martín
authors.forename: M L
authors.initials: ML
authors.lastname: Candenas
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D010052:Ovariectomy; D016133:Polymerase Chain Reaction; D011374:Progesterone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018042:Receptors, Neurokinin-3 / Q000187:drug effects* / Q000235:genetics; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D004967:Estrogens; D012333:RNA, Messenger; D018042:Receptors, Neurokinin-3; D011374:Progesterone