Studies on tumor-promoting activity of polyethylene: inhibitory activity of metabolic cooperation on polyethylene surfaces is markedly decreased by surface modification with collagen but not with RGDS peptide.
Tumor promotion activity of polyethylene (PE) was estimated in terms of the inhibitory potentials on the gap-junctional intercellular communication using the metabolic cooperation assay. The gap-junctional intercellular communication of test cells was inhibited on the PE film, but this inhibitory activity was markedly decreased when the surface of the PE film was immobilized with collagen. These results suggest that the in vivo tumor promotion activity of the untreated PE may be stronger than that of collagen-immobilized PE. On the other hand, surface modification with RGDS peptide, which is well known as the sequence of cell attachment domain in extracellular matrix proteins, did not reduce the promotion activity of PE film. In addition, neither modification with bovine serum albumin nor RGES peptide reduced the activity of PE film. These findings suggest that reduction of the inhibitory activity on gap-junctional intercellular communication by collagen immobilization is not simply due to improved cell adherence via the RGDS sequence but to some cell-cell recognition via collagen molecules essential for the gap-junctional intercellular communication.
Nakaoka
R
R
Division of Medical Devices, National Institute of Health Sciences, Tokyo, Japan.
Tsuchiya
T
T
Kato
K
K
Ikada
Y
Y
Nakamura
A
A
D000818:Animals; D002273:Carcinogens; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D017629:Gap Junctions / Q000187:drug effects*; D008168:Lung; D009842:Oligopeptides / Q000494:pharmacology*; D011095:Polyethylenes / Q000737:chemistry / Q000633:toxicity*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013499:Surface Properties
D002273:Carcinogens; D009842:Oligopeptides; D011095:Polyethylenes; D012710:Serum Albumin, Bovine; D003094:Collagen; C046617:arginyl-glycyl-aspartyl-serine