Porcine interferon-gamma inhibits the growth of Legionella pneumophila in WiREF cells in vitro.
The intracellular growth of Legionella pneumophila in WiREF (Wistar rat embryonal fibroblast) cells was inhibited by porcine interferon-gamma. The effect was compared with that of different human interferons (alpha and gamma). The growth inhibition was dose-dependent and required the pretreatment of WiREF cells with interferon. The development of an antibacterial state of the cells was observed. When interferon was added together with bacteria or 1 d after the infection there was no inhibition. Also, there was no direct antibacterial effect of the interferon. In addition, cell pretreatment with a combination of interferon and antibiotics failed to show a synergistic effect.
Eberl-Gregoric
E
E
Institute for Microbiology and Immunology, Medical Faculty, Ljubljana, Slovenia.
Filipic
B
B
Rozman
S
S
Cencic
A
A
Drinovec
B
B
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D007370:Interferon Type I / Q000008:administration & dosage / Q000494:pharmacology; D007371:Interferon-gamma / Q000008:administration & dosage / Q000494:pharmacology*; D016952:Legionella pneumophila / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D011994:Recombinant Proteins; D013552:Swine
D000900:Anti-Bacterial Agents; D007370:Interferon Type I; D011994:Recombinant Proteins; D007371:Interferon-gamma