Role of oxygen-derived free radicals in free growth retardation induced by ischemia-reperfusion in rats.
We investigated the involvement of oxygen-derived free radicals in the pathogenesis of the intrauterine growth retardation (IUGR) induced in Sprague-Dawley rats by ischemia-reperfusion. On day 17 of gestation, rats received saline, superoxide dismutase (SOD, 50,000 U/kg), catalase (CAT, 50,000 U/kg), or SOD + CAT subcutaneously 1 h before induction of 30 min of ischemia of the right uterine horn. On day 21 the placental level of lipid peroxides was significantly increased (P < 0.001 vs. sham-operated group) and IUGR was induced (P < 0.001 vs. left horn) in the saline-treated group n = 6). Pretreatment with SOD + CAT (n = 6) significantly inhibited the increase in placental lipid peroxides and prevented IUGR. The effect of ischemia-reperfusion on uterine blood flow, with or without pretreatment with radical scavengers, was investigated in separate experiments by laser-Doppler flowmetry. The induction of hypoperfusion 3 h after ischemia (blood flow -40 +/- 5%, n = 6, P < 0.05) was blocked by pretreatment with SOD + CAT (n = 6). Results indicate that oxygen-derived free radicals may be important in the development of postischemic uteroplacental hypoperfusion and of ischemia-reperfusion-induced IUGR in the rat.
Ishimoto
H
H
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
Natori
M
M
Tanaka
M
M
Miyazaki
T
T
Kobayashi
T
T
Yoshimura
Y
Y
D000818:Animals; D001835:Body Weight; D036861:Delivery, Obstetric; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology* / Q000473:pathology; D005333:Fetus / Q000033:anatomy & histology; D016166:Free Radical Scavengers / Q000494:pharmacology; D007511:Ischemia / Q000150:complications*; D008054:Lipid Peroxides / Q000378:metabolism; D009929:Organ Size; D010920:Placenta / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000502:physiology*; D012039:Regional Blood Flow; D015424:Reperfusion; D014599:Uterus / Q000098:blood supply*
D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species