The development and characterisation of a SV40 T-antigen positive cell line of human hepatic origin.
COS7 cells and other cell lines expressing the SV40 large T-antigen, have been of great benefit in transfection studies using transient expression vectors which contain the SV40 origin of replication, as these cells allow plasmid replication to a high copy number. As no T-antigen expressing cell line derived from a well characterised hepatocyte-like continuous cell line currently exists, the establishment of such a cell line for studies which require expression of hepatocyte-specific factors would be extremely useful. A HepG2-derived stable cell line (THT1) was therefore developed which demonstrates a high level of transfection efficiency whilst retaining hepatocyte-like features, such as the production of hepatitis B virus. The THT1 cell line displayed chromosomal integration of the SV40 T-antigen gene and nuclear expression of the antigen. The cell line also maintained the general morphological features of its parent cell line, and showed an increased rate of cell growth.
Harvey
T J
TJ
Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston, QLD, Australia.
Macnaughton
T B
TB
Gowans
E J
EJ
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis* / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000096:biosynthesis; D015139:Blotting, Southern; D019556:COS Cells / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D002460:Cell Line / Q000276:immunology*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis; D006515:Hepatitis B virus / Q000235:genetics; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D008099:Liver / Q000166:cytology*; D014176:Protein Biosynthesis; D014162:Transfection; D014779:Virus Replication / Q000502:physiology
D000952:Antigens, Polyomavirus Transforming; D000956:Antigens, Viral; D006514:Hepatitis B Surface Antigens; D015500:Chloramphenicol O-Acetyltransferase