Distribution of sphingosine 1-phosphate, a bioactive sphingolipid, in rat tissues.
Although a growing number of reports on the bioactivities of sphingosine 1-phosphate (Sph-1-P) in various systems have been appearing recently, current evidence for the involvement of Sph-1-P in signal transduction or cellular function(s) consists largely of data on cellular and biochemical effects of exogenous Sph-1-P. In this study, we measured Sph-1-P contents in various rat tissues. When the amount of this sphingoid base was measured by its N-acetylation into [3H]C2-ceramide 1-phosphate with [3H]acetic anhydride, we could detect Sph-1-P in all rat tissues examined. We also got unexpected findings that Sph-1-P exists most abundantly in testis = intestine > spleen (in the order of Sph-1-P abundance when its amounts were adjusted to the phospholipid levels). Our new data on Sph-1-P distribution in rat tissues may imply novel bioactivities for Sph-1-P.
Yatomi
Y
Y
Department of Pathobiology, University of Washington, Seattle 98195, USA.
Welch
R J
RJ
Igarashi
Y
Y
D000107:Acetylation; D000818:Animals; D005260:Female; D007422:Intestines / Q000737:chemistry; D008246:Lysophospholipids; D008297:Male; D009928:Organ Specificity; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000032:analysis; D013154:Spleen / Q000737:chemistry; D013737:Testis / Q000737:chemistry; D014316:Tritium
D008246:Lysophospholipids; D010743:Phospholipids; D014316:Tritium; C060506:sphingosine 1-phosphate; D013110:Sphingosine