A rapid and simple method for extracting human immunodeficiency virus type 1 RNA from plasma: enhanced sensitivity.
We present here a rapid and simple technique for processing human immunodeficiency virus (HIV)-infected plasma by high-speed centrifugation. HIV type 1 virions are pelleted from up to 1 ml of plasma, gently lysed with a nonionic detergent, and directly amplified. The procedure has few manipulations and requires approximately 1.5 h for the processing of 24 samples. Viral recovery ranges from 80 to 90%, with an analytical sensitivity approaching 20 copies/ml.
Mulder
J
J
Roche Molecular Systems, Alameda, San Francisco, California, USA.
Resnick
R
R
Saget
B
B
Scheibel
S
S
Herman
S
S
Payne
H
H
Harrigan
R
R
Kwok
S
S
D015658:HIV Infections / Q000097:blood / Q000821:virology*; D015497:HIV-1; D006801:Humans; D012367:RNA, Viral / Q000097:blood / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
D012367:RNA, Viral