'Semifree-floating' treatment: a simple and fast method to process consecutive sections for immunohistochemistry and neuronal tracing.
A method is described which allows for histochemical processing of thick (50-200 microm) and consecutive sections of neural tissue, a prerequisite for many neuroanatomical studies. Two examples are given: (A) biotin-dextran-amine (BDA) tracing of neuronal connections in 50-100 microm thick vibratome sections of the adult rat brain and (B) immunohistochemical analysis of tyrosine hydroxylase-positive bulbospinal fibers in 50 microm thick cryosections of spinal cord.
Herzog
A
A
Brain Research Institute, University of Zurich, Switzerland.
Brösamle
C
C
D000818:Animals; D001710:Biotin; D002540:Cerebral Cortex / Q000737:chemistry / Q000201:enzymology; D003911:Dextrans; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D018613:Microscopy, Confocal; D008867:Microtomy / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000737:chemistry / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D003911:Dextrans; D001710:Biotin; D014446:Tyrosine 3-Monooxygenase