High-level expression of cecropin CMIV in E. coli from a fusion construct containing the human tumor necrosis factor.
Cecropin CMIV gene was fused to the 3'-terminus of the mutated tumor necrosis factor (TNFb) gene and the fusion gene was directly under the control of an inducible T7 promoter in pET-11d. This fusion gene was overexpressed in Escherichia coli with an expression level of approximate 40%-50% of total cellular proteins, and was produced mainly in the form of inclusion body. Peptide with antibacterial activity was obtained by cleaving the fusion protein with CNBr.
Wang
L
L
Department of Biochemistry, Nanjing University, P.R. China.
Wu
H
H
Dou
F
F
Xie
W
W
Xu
X
X
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007301:Insect Hormones / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics*
D007301:Insect Hormones; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha