title: FOS expression in grafted gonadotropin-releasing hormone neurons in hypogonadal mouse: mating and steroid induction.
abstract: We used FOS expression, widely accepted as a marker for neuronal activation, to evaluate physiologically induced activation of gonadotropin-releasing hormone (GnRH) neurons within intraventricular preoptic area grafts in hypogonadal (hpg) female mice. Hpg mice lack endogenous GnRH due to a mutated gene, but can respond to grafted GnRH neurons with reproductive development. The purpose of this study was to determine the degree to which the host brain regulates grafted GnRH neurons. FOS expression in grafted GnRH neurons was induced in progesterone-primed female mice paired with sexually active males. The degree of sexual activity did not affect the outcome, with 40.9 +/- 12.2% of the grafted GnRH cells expressing FOS when male partners performed intromissions, and 47.5 +/- 10.2% when they also ejaculated. There was little or no FOS expression in the grafts of unprimed hpg mice paired with sexually active males, in unpaired mice primed with progesterone or sequential estradiol benzoate and progesterone, or in controls. The pattern of FOS expression in the brains of the female hpg mice engaged in mating behavior was similar to that reported in other species, with moderate to high expression in the medial preoptic area, ventromedial nucleus, and medial amygdala in females paired with males that ejaculated. The present results support the hypothesis that host-derived activation of grafted GnRH neurons underlies aspects of reproductive responses seen in hpg mice with grafts, and further, that at least a portion of the host-graft connectivity is steroid sensitive.
authors.affiliation: Department of Anatomy and Cell Biology, Columbia University, College of Physicians & Surgeons, New York, New York 10032, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Wu
authors.forename: A J
authors.initials: AJ
authors.lastname: Silverman
authors.forename: M J
authors.initials: MJ
authors.lastname: Gibson
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002584:Cervix Uteri / Q000187:drug effects; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007006:Hypogonadism / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009474:Neurons / Q000378:metabolism* / Q000637:transplantation; D010062:Ovulation Induction / Q000379:methods; D011301:Preoptic Area / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D012726:Sexual Behavior, Animal / Q000502:physiology*; D014621:Vagina / Q000187:drug effects
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone