title: Quantitative determination of theophylline by an automated chemiluminescent immunoassay in serum and plasma: comparison to other methods of analysis.
abstract: A chemiluminescent immunoassay for theophylline in serum or plasma was developed for the Ciba Corning ACS: 180 automated analyzer. The assay has a limit of quantitation of 0.2 microgram/ml, with a range up to 40 micrograms/ml. The cross-reactivity to metabolites 1,3-dimethyluric acid 3-methylxanthine was 3.4% and 2.5%, respectively. Overall means of 101.0% and 97.8% were determined from dilution linearity and addition studies, respectively. When compared to a high-performance liquid chromatography (HPLC) method, a linear regression of ACS Theophylline = 0.996 (HPLC) + 0.35, r = 0.991, n = 93, was obtained. Similar results were obtained when the ACS assay was compared to other immunoassays. Precision (within-run and total coefficient of variation or CV) was < 6% in the therapeutic range (10-20 micrograms/ml). The performance data demonstrate that the ACS Theophylline assay provides an additional choice for the clinical measurement of the drug.
authors.affiliation: Ciba Corning Diagnostics Corporation, East Walpole, Massachusetts 02032, USA.
authors.forename: D P
authors.initials: DP
authors.lastname: Malliaros
authors.forename: S S
authors.initials: SS
authors.lastname: Wong
authors.forename: A H
authors.initials: AH
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Campbell
authors.forename: H
authors.initials: H
authors.lastname: Leonard
authors.forename: S
authors.initials: S
authors.lastname: Houser
authors.forename: M
authors.initials: M
authors.lastname: Berg
authors.forename: T
authors.initials: T
authors.lastname: Gornet
authors.forename: C
authors.initials: C
authors.lastname: Brown
authors.forename: Y J
authors.initials: YJ
authors.lastname: Feng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D017248:Enzyme Multiplied Immunoassay Technique; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D006801:Humans; D013806:Theophylline / Q000097:blood*
chemical_list: D013806:Theophylline