Measurement of plasma ketoprofen by a rapid high-performance liquid chromatography assay.
The authors developed a high performance liquid chromatography assay for the determination of plasma ketoprofen concentration using a single-step extraction, a short chromatographic separation of 3 min, and a sample volume of 50 microliters. The assay possessed linearity up to 500 mg/l, sensitivity down to at least 1 mg/l, average recovery of 100.1%, and run-to-run precision (n = 20) of 4.6% at a level of 10 mg/l and 2.2% at a level of 40 mg/l. Furthermore, the assay was free of interference from 51 prescription and over-the-counter medications. The authors conclude that the method described here is ideally suited for the determination of ketoprofen concentration in a clinical laboratory setting for the purpose of therapeutic monitoring and assessment of toxicity.
Rifai
N
N
Department of Laboratory Medicine, Children's Hospital, Boston, MA 02115, USA.
Lafi
M
M
Sakamoto
M
M
Law
T
T
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007660:Ketoprofen / Q000097:blood*
D007660:Ketoprofen