title: Serine palmitoyltransferase (scs1/lcb2) mutants have elevated copy number of the L-A dsRNA virus.
abstract: The microsomal fraction isolated form serine palmitoyltransferase (lcb2/scs1) mutants is enriched in a 90 kDa protein. The protein was identified as the major coat (Gag) protein of the L-A dsRNA virus particles by partial sequencing and by its interaction with anti-Gag antibodies. The total amount of Gag in whole-cell lysates of scs1/lcb2 mutant cells is greater than in wild-type lysates indicating that the enrichment of the protein in the microsomal fraction of scs1/lcb2 mutant cells may result from increased copy number of the L-A dsRNA virus. This is supported by the findings that the mutants also have increased levels of L-A dsRNA. Altered sphingolipid synthesis in the scs1 mutant cells appears to increase the copy number of the L-A viral particles.
authors.affiliation: Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
authors.forename: V R
authors.initials: VR
authors.lastname: Garnepudi
authors.forename: C
authors.initials: C
authors.lastname: Zhao
authors.forename: T
authors.initials: T
authors.lastname: Beeler
authors.forename: T
authors.initials: T
authors.lastname: Dunn
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D000914:Antibodies, Viral; D002213:Capsid / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D015683:Gene Products, gag / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D008861:Microsomes / Q000821:virology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation / Q000502:physiology; D010446:Peptide Fragments / Q000737:chemistry; D012328:RNA Viruses / Q000276:immunology / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000821:virology*; D029701:Saccharomyces cerevisiae Proteins; D017421:Sequence Analysis; D051102:Serine C-Palmitoyltransferase; D013107:Sphingolipids / Q000096:biosynthesis
chemical_list: D000914:Antibodies, Viral; D015683:Gene Products, gag; D010446:Peptide Fragments; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D013107:Sphingolipids; D000217:Acyltransferases; C495087:LCB2 protein, S cerevisiae; D051102:Serine C-Palmitoyltransferase