Binding of the type 3 fimbriae of Klebsiella pneumoniae to human endothelial and urinary bladder cells.
Binding of the two identified type 3 fimbrial variants of Klebsiella pneumoniae to human endothelial EA-hy926 and bladder T24 cells was assessed. The recombinant Escherichia coli strain LE392(pFK12), expressing plasmid-encoded type 3 fimbriae of K. pneumoniae, adhered to both cell lines, and the fimbriae purified from the strain bound to both cell lines in a dose-dependent manner. Adhesiveness to both cell lines of chromosomally encoded type 3 fimbriae from K. pneumoniae IApc35 was lower. No binding was detected with type 1 fimbriae of K. pneumoniae. Both type 3 fimbrial variants exhibited a significantly lower affinity for the cell lines than did S fimbriae of meningitis-associated E. coli.
Tarkkanen
A M
AM
Department of Biosciences, FIN-00014 University of Helsinki, Finland.
Virkola
R
R
Clegg
S
S
Korhonen
T K
TK
D002460:Cell Line; D004727:Endothelium / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D001743:Urinary Bladder / Q000382:microbiology*
D011994:Recombinant Proteins