title: Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element.
abstract: In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H2O2 stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A2 (PLA2) in the H2O2-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA2, blocked H2O2-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A2' in the signaling pathway of H2O2 to SRE.
authors.affiliation: Laboratory of Molecular and Cellular Genetics, Institute of Environment and Life Science, Hallym University, Chun-Cheon, South Korea. jhkim@sun.hallym.ac.kr
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Kwack
authors.forename: S E
authors.initials: SE
authors.lastname: Choi
authors.forename: B C
authors.initials: BC
authors.lastname: Kim
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: I J
authors.initials: IJ
authors.lastname: Kang
authors.forename: C C
authors.initials: CC
authors.lastname: Kumar
mesh_terms: D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D051381:Rats; D026362:Serum Response Factor; D015398:Signal Transduction
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D026362:Serum Response Factor; D006861:Hydrogen Peroxide; D010741:Phospholipases A; D054467:Phospholipases A2; D020558:GTP Phosphohydrolases