title: An investigation of potential desensitizing agents in the dentine disc model: a scanning electron microscopy study.
abstract: Cervical dentine sensitivity (CDS) may be defined as pain arising from exposed dentine. The prefix cervical indicates the location of the sensitivity and/or its subsequent treatment. Currently the most accepted mechanism of intradental nerve activation associated with dentine sensitivity appears to be hydrodynamic in nature. The concept of tubule occlusion as a method of dentine desensitization is a logical conclusion of the hydrodynamic theory. The authors employed the dentine disc model, qualitative scanning electron microscopy (SEM) and X-ray microanalysis to investigate whether selected desensitizing agents occlude dentinal tubule orifices. Strict control procedures have been used together with various methods of application to apply these agents to human dentine discs. SEM was used to examine the degree of deposit left by the various agents on disc surfaces and X-ray microanalysis was employed to characterize the elemental composition of the deposit. Analysis of selected agents, both prior to and after application on dentine discs was performed for comparative purposes. The degree of retention of the surface deposit upon rotation with saliva supernatant for 6 h was also studied. The results of this study indicated that ferric oxalate, the active ingredient of Sensodyne Sealant, which produced initial crystal-like structures, occluding almost all the tubule orifices was superior to potassium oxalate (Butler Protect). Of the over-the-counter (OTC) desensitizing products tested, both silica- and calcium-based abrasive components were observed both on the surface and within the tubules, indicating a certain degree of therapeutic potential for these two components. These findings suggest that certain desensitizing agents have tubule occluding properties as observed in this in vitro system which, in turn, may indicate a therapeutic potential in vivo.
authors.affiliation: Department of Periodontology, Eastman Dental Institute, University of London, U.K.
authors.forename: T Y
authors.initials: TY
authors.lastname: Ling
authors.forename: D G
authors.initials: DG
authors.lastname: Gillam
authors.forename: P M
authors.initials: PM
authors.lastname: Barber
authors.forename: N J
authors.initials: NJ
authors.lastname: Mordan
authors.forename: J
authors.initials: J
authors.lastname: Critchell
mesh_terms: D002118:Calcium / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use; D003460:Crystallization; D003804:Dentin / Q000187:drug effects* / Q000294:innervation / Q000648:ultrastructure; D003807:Dentin Sensitivity / Q000188:drug therapy*; D004577:Electron Probe Microanalysis; D006801:Humans; D008855:Microscopy, Electron, Scanning; D004366:Nonprescription Drugs / Q000032:analysis / Q000627:therapeutic use; D010070:Oxalates / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use; D012399:Rotation; D012463:Saliva / Q000502:physiology; D012822:Silicon Dioxide / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use; D019237:Tooth Cervix / Q000187:drug effects / Q000294:innervation / Q000648:ultrastructure; D014100:Toothpastes / Q000032:analysis / Q000627:therapeutic use*
chemical_list: D004366:Nonprescription Drugs; D010070:Oxalates; D014100:Toothpastes; D012822:Silicon Dioxide; D002118:Calcium