Human immunoglobulin isotype and IgG subclass response to different antigens of Moraxella catarrhalis.
Enzyme immunoassays were tested for the determination of antibodies of different isotypes and IgG subclasses to Moraxella catarrhalis in human sera. An assay based on an outer membrane protein preparation (OMP) as antigen was compared to assays using whole bacterial cells and a purified lipopolysaccharide preparation. There was a good correlation between the results obtained with the OMP preparation and the whole-cell antigen. In paired sera, optimal sensitivity was obtained by using the OMP preparation as coating antigen and testing for a rise in IgG3 antibodies. However, patients with high levels of antibodies in acute serum had no or only an insignificant antibody response during infection.
Rahman
M
M
Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden.
Holme
T
T
Jönsson
I
I
Krook
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001991:Bronchitis / Q000276:immunology / Q000382:microbiology; D004646:Emphysema / Q000150:complications; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000145:classification; D007132:Immunoglobulin Isotypes / Q000097:blood*; D008173:Lung Diseases, Obstructive / Q000150:complications; D008297:Male; D008875:Middle Aged; D001936:Moraxella catarrhalis / Q000276:immunology* / Q000302:isolation & purification; D011014:Pneumonia / Q000276:immunology / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000209:etiology / Q000276:immunology / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes