[Sairei-to may inhibit the synthesis of endothelin-1 in nephritic glomeruli].
In order to clarify the antinephritic mechanisms of Sairei-to (TJ-114), its effects on the synthesis and expression of endothelin-1 were evaluated in rats with anti-glomerular basement membrane (GBM) nephritis. TJ-114 was administered orally once daily from the 20th day after anti-GBM serum injection and was continued throughout the experiment. TJ-114 prevented proteinuria and histopathological changes in the glomeruli of nephritic rats. TJ-114 inhibited elevation of the endothelin-1 concentration in the supernatant from cultured glomeruli of nephritic rats and the endothelin-1 positive area in the glomeruli. TJ-114 inhibited the elevation of systolic blood pressure and the number of proliferating cell nuclear antigen (PCNA)-positive cells per glomeruli. We also found that the constitutive Kampo medicine in TJ-114, Gorei-san (TJ-17), inhibited the synthesis and expression of endothelin-1. In addition, the constitutive herbs TJ-17, alismatis rhizoma (Japanese name "Takusha") and hoelen (Japanese name "Bukuryou") inhibited the synthesis and expression of endothelin-1. These results indicate that the antinephritic actions of TJ-114 may be due partially to the inhibition of endothelin-1 synthesis in the glomeruli.
Hattori
T
T
TSUMURA & Co. Central Laboratories, New Drug Research Department 1.
Fujitsuka
N
N
Kurogi
A
A
Shindo
S
S
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D019332:Endothelin-1 / Q000096:biosynthesis*; D005921:Glomerulonephritis / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; C080903:sairei-to