Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR.
A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of 141 isolates, including the 5 group O isolates that were not detected with either of the AMPLICOR HIV-1 test formats.
Respess
R A
RA
Roche Molecular Systems, Inc., Alameda, California 94501, USA.
Butcher
A
A
Wang
H
H
Chaowanachan
T
T
Young
N
N
Shaffer
N
N
Mastro
T D
TD
Biryahwaho
B
B
Downing
R
R
Tanuri
A
A
Schechter
M
M
Pascu
R
R
Zekeng
L
L
Kaptué
L
L
Gürtler
L
L
Eberle
J
J
Ellenberger
D
D
Fridlund
C
C
Rayfield
M
M
Kwok
S
S
D000328:Adult; D014644:Genetic Variation; D016679:Genome, Viral; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*