Development of a simple restriction fragment length polymorphism (RFLP) based assay for HCV genotyping and comparative analysis with genotyping and serotyping tests.
The distribution between the different hepatitis C virus genotypes and subtypes has potentially important clinical and epidemiological implications. With this view a simple restriction fragment length polymorphism (RFLP) based assay was developed to identify the three major genotypes, 1, 2 and 3 and distinguish subtype 1a from 1b. This RFLP test uses a combination of three restriction endonuclease digestions, BstN1, BstU1 and Sau3a, respectively. Comparison with a 5'UTR based assay (LiPA), showed a 98% concordance with the RFLP based assay. In addition, good concordance is shown between these data and those obtained with a serological assay based on NS4 peptides.
Thiers
V
V
Hybridotest, CBMS, Institut Pasteur, Paris, France.
Jaffredo
F
F
Tuveri
R
R
Chodan
N
N
Bréchot
C
C
D000918:Antibody Specificity; D015342:DNA Probes / Q000032:analysis / Q000235:genetics; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping / Q000145:classification*
D015342:DNA Probes