Limited junctional diversity of V delta 5-J delta 1 rearrangement in multiple sclerosis patients.
T-cell receptor (TCR) delta gene repertoire, as assessed by V delta-J delta rearrangements, has been analyzed in nine multiple sclerosis (MS) cases and in 30 healthy individuals by seminested PCR technique. Among the V delta-J delta junctional diversities studied, the most striking result has been observed in V delta 5-J delta 1 rearrangement. The detection of repeated V delta 5-J delta 1 nucleotide sequences in all analyzed clones from seven out of nine patients studied proved the monoclonal nature of gamma delta T-cells with V delta 5-J delta 1 rearrangement. The clonal nature of this rearrangement proved by PAGE and sequencing analysis may suggest an antigen-driven expansion of gamma delta T cells and argues for a significant role of gamma delta T-cells with V delta 5-J delta 1 rearrangement in MS pathogenesis. However, it cannot be excluded that clonal expansion of these lymphocytes may represent secondary change to central nervous system damage.
Nowak
J S
JS
Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
Michałowska-Wender
G
G
Januszkiewicz
D
D
Wender
M
M
D000328:Adult; D001483:Base Sequence; D017931:DNA Primers; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D014644:Genetic Variation; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics* / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values
D017931:DNA Primers