Molecular characterization of a dominantly inherited bleeding disorder with impaired platelet responses to thromboxane A2.
Thromboxane A2 (TXA2) is a major arachidonic acid metabolite of platelets and induces platelet functions by binding to specific receptors on the membrane. We have found patients with hemostatic defects due to impaired platelet responses to TXA2, and molecular characterization of the patients has been carried out. Platelets from these two unrelated patients showed impaired aggregation responses to TXA2 and its analogues despite the normal response to thrombin. Although the patients' platelets exhibited normal binding activities to TXA2 analogues, they showed decreased GTPase activity and second messenger formation when stimulated by STA2, a stable TXA2 agonist. To understand the molecular basis of this abnormality, we determined the cDNA sequence of the TXA2 receptor by reverse transcription-polymerase chain reaction (RT-PCR) from the patient's platelet RNA, and identified a single amino acid substitution (Arg60 for Leu) in the first cytoplasmic loop of the receptor. This mutation was found in both isoforms of the platelet TXA2 receptor which we have recently found: TXR alpha with the same structure as the placental TXA2 receptor and TXR beta with the same structure as the endothelial TXA2 receptor, and was detected exclusively in affected members of two unrelated families with the disorder. The mutant TXR alpha and TXR beta expressed in COS-m6 cells showed decreased agonist-induced phospholipase C activation despite their normal ligand binding affinities. These results suggest that the Arg60 for Leu mutation is responsible for the disorder and imply a critical role for the first cytoplasmic loop in the interaction of the TXA2 receptor with the G protein.
Okuma
M
M
Department of Internal Medicine, Kyoto University, Faculty of Medicine, Japan.
Hirata
T
T
Ushikubi
F
F
Kakizuka
A
A
Narumiya
S
S
D001483:Base Sequence; D001791:Blood Platelet Disorders / Q000097:blood / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D010974:Platelet Aggregation / Q000187:drug effects; D016133:Polymerase Chain Reaction; D017482:Receptors, Thromboxane / Q000097:blood / Q000235:genetics*; D013928:Thromboxane A2 / Q000494:pharmacology*
D017482:Receptors, Thromboxane; D013928:Thromboxane A2