[Anti-endomysium and anti-reticulin antibodies in adults with celiac disease followed-up in the Paris area].
OBJECTIVE
Circulating IgA class anti-endomysium and anti-reticulin antibodies are reliable serological markers for the diagnosis of coeliac disease. The aim of this study was to evaluate their diagnostic sensitivity and specificity, and their prevalence in a group of coeliac adults taking a gluten-free diet followed in Ile-de-France.


METHODS
One hundred and forty-five sera from 134 patients were examined for IgA and IgG anti-endomysium and anti-reticulin antibodies by indirect immunofluorescence. Sixty-eight patients were confirmed coeliacs; 8 patients with noncoeliac (unresponsive to a gluten-free diet) duodenal villous atrophy and 58 patients with no villous atrophy served as controls.


RESULTS
In active coeliac disease, the sensitivity of anti-endomysium and anti-reticulin antibodies was 88% and 50%, respectively. Their specificity was 100%: they were never detected in case of noncoeliac villous atrophy. Under a strict gluten-free diet, their prevalence was 7% and 0%, respectively.


CONCLUSIONS
In the studied population, the diagnosis accuracy of IgA anti-endomysium antibodies confirms their usefulness in screening and follow-up of coeliac disease. The absence of anti-endomysium and anti-reticulin antibodies in those cases with noncoeliac villous atrophy suggests that in the latter, different immunopathologic mechanisms, as compared with coeliac disease, are concerned.
Boige
V
V
Service d'Hépato-Gastroentérologie, Hôpital Saint-Louis, Paris.
Bouhnik
Y
Y
Delchier
J C
JC
Jian
R
R
Matuchansky
C
C
André
C
C
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D002446:Celiac Disease / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005500:Follow-Up Studies; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010297:Paris; D011237:Predictive Value of Tests; D012155:Reticulin / Q000276:immunology*; D012680:Sensitivity and Specificity
D000906:Antibodies; D015415:Biomarkers; D007070:Immunoglobulin A; D012155:Reticulin