Phosphorylation-dependent control of structures of intermediate filaments: a novel approach using site- and phosphorylation state-specific antibodies.
Site-specific phosphorylation of intermediate filament (IF) proteins on serine and threonine residues leads to dynamic alterations in filament structure. Site- and phosphorylation state-specific antibodies for IF proteins can visualize spatial and temporal distribution of site-specific IF protein phosphorylations in the cell. These antibodies are also useful to identity IF kinases involved in cellular events, including cell signaling and cell cycle.
Inagaki
M
M
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya.
Inagaki
N
N
Takahashi
T
T
Takai
Y
Y
D000906:Antibodies / Q000737:chemistry*; D007381:Intermediate Filament Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D011487:Protein Conformation; D011494:Protein Kinases / Q000378:metabolism
D000906:Antibodies; D007381:Intermediate Filament Proteins; D011494:Protein Kinases