title: Exacerbations of clinical symptoms in human immunodeficiency virus type 1-infected patients with multicentric Castleman's disease are associated with a high increase in Kaposi's sarcoma herpesvirus DNA load in peripheral blood mononuclear cells.
abstract: The epidemiologic link between multicentric Castleman's disease (MCD) and Kaposi's sarcoma (KS) and the high frequency of KS herpesvirus (KSHV) detection in both diseases raise the question of a role of this new virus in the pathogenesis of MCD. To explore this hypothesis, the KSHV DNA load was investigated in peripheral blood mononuclear cells of 3 human immunodeficiency virus (HIV)-infected patients with MCD at different points during the clinical course. Clinical parameters, such as fever and the presence of lymphadenopathy, were systematically assessed. Hemogram and C-reactive protein level determinations were performed as standard procedures. KSHV DNA load was investigated by means of semiquantitative polymerase chain reaction assay using peripheral blood mononuclear cells of the patients. A correlation between the variation in clinical and biologic parameters related to MCD and KSHV DNA load was found, suggesting a close relationship between KSHV and MCD in HIV-1-infected patients.
authors.affiliation: Laboratoire de Virologie, CNRS EP 57, Hôpital Tarnier, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Grandadam
authors.forename: N
authors.initials: N
authors.lastname: Dupin
authors.forename: V
authors.initials: V
authors.lastname: Calvez
authors.forename: I
authors.initials: I
authors.lastname: Gorin
authors.forename: L
authors.initials: L
authors.lastname: Blum
authors.forename: S
authors.initials: S
authors.lastname: Kernbaum
authors.forename: D
authors.initials: D
authors.lastname: Sicard
authors.forename: Y
authors.initials: Y
authors.lastname: Buisson
authors.forename: H
authors.initials: H
authors.lastname: Agut
authors.forename: J P
authors.initials: JP
authors.lastname: Escande
authors.forename: J M
authors.initials: JM
authors.lastname: Huraux
mesh_terms: D005871:Castleman Disease / Q000150:complications* / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D006679:HIV Seropositivity / Q000150:complications* / Q000503:physiopathology* / Q000821:virology; D015497:HIV-1; D006566:Herpesviridae Infections / Q000097:blood / Q000150:complications*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral