DNA-alginate complex recognized by autoantibodies against DNA.
Double-stranded DNA was effectively complexed with alginic acid and immobilized on a surface of polystyrene microtiter plate. Dose-dependent binding of anti-DNA autoantibodies was finely observed to the solid phase DNA-alginate complex in enzyme-linked immunosorbent assay (ELISA). In contrast, non-specific binding of antibodies to alginate was scarcely detected rather than to poly-L-lysine. These results shown an availability of the solid phase DNA-alginate complex as an antigen in ELISA for detection of anti-DNA antibodies.
Kitamura
H
H
Division of Bioscience, Graduate school of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Matsuura
E
E
Nagata
A
A
Sakairi
N
N
Tokura
S
S
Nishi
N
N
D000464:Alginates / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001323:Autoantibodies / Q000032:analysis* / Q000378:metabolism; D004247:DNA / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005189:False Positive Reactions; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology; D008239:Lysine / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D051379:Mice
D000464:Alginates; D001323:Autoantibodies; D006603:Hexuronic Acids; D007106:Immune Sera; D020723:Glucuronic Acid; D004247:DNA; D008239:Lysine