title: Quantification of urinary uric acid in the presence of thymol and thimerosal by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic method was developed as an alternative to automated enzymatic analysis of uric acid in human urine preserved with thymol and/or thimerosal. Uric acid (tR = 10 min) and creatinine (tR = 5 min) were separated and quantified during isocratic elution (0.025 M acetate buffer, pH 4.5) from a mu Bondapak C18 column. The uric-acid peak was identified chemically by incubating urine samples with uricase. The thymol/thimerosal peak appeared at 31 min during the washing step and did not interfere with the analysis. We validated the high-performance liquid chromatographic method for linearity, precision and accuracy, and the results were found to be excellent.
authors.affiliation: KRUG Life Sciences Inc., Houston, TX 77058, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: R A
authors.initials: RA
authors.lastname: Pietrzyk
authors.forename: P A
authors.initials: PA
authors.lastname: Whitson
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003404:Creatinine / Q000652:urine; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011310:Preservatives, Pharmaceutical; D012680:Sensitivity and Specificity; D013849:Thimerosal; D013943:Thymol; D014527:Uric Acid / Q000652:urine*
chemical_list: D011310:Preservatives, Pharmaceutical; D013849:Thimerosal; D014527:Uric Acid; D013943:Thymol; D003404:Creatinine