title: Localization of secretory phospholipase A2 in mouse cochlear tissues and cultured cells.
abstract: OBJECTIVE
  To localize the expression of secretory phospholipase A2 (sPLA2) in the cochlear duct.
  
  
  METHODS
  By means of an immunocytochemistry technique that used rabbit anti-porcine pancreatic phospholipase A2 antiserum, sPLA2 sites were identified in vivo and in vitro in the adult mouse cochlear duct.
  
  
  METHODS
  Eight C57BL/6N normal adult male mice (11-12 weeks old) (6 mice for the in vivo study, 2 mice for the in vitro study).
  
  
  RESULTS
  Different patterns of immunostaining for sPLA2 were observed in tissues of the cochlear duct. High levels of sPLA2 were detected in the following areas: the basal cells of the stria vascularis, the organ of Corti, the spiral ganglion, and the cochlear nerve; moderate levels of sPLA2 were found in the suprastrial and poststrial portions of the spiral ligament; low levels of sPLA2 were observed in the marginal and intermediate cells of the stria vascularis and the infrastrial portion of the spiral ligament. Immunoreactivity for sPLA2 also was detected in cultured marginal cells of the stria vascularis and in fibrocytes of the spiral ligament.
  
  
  CONCLUSIONS
  Secretory phospholipase A2 is present in mouse cochlear tissues and individual cultured cells. These results suggest that the levels of sPLA2 expression in the cochlear duct are cell specific.
authors.affiliation: Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, USA.
authors.forename: J
authors.initials: J
authors.lastname: Zuo
authors.forename: K E
authors.initials: KE
authors.lastname: Rarey
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003051:Cochlea / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010741:Phospholipases A / Q000096:biosynthesis*; D054467:Phospholipases A2
chemical_list: D010741:Phospholipases A; D054467:Phospholipases A2