In vivo effect of alpha 1-acid glycoprotein on experimentally enhanced capillary permeability in guinea-pig skin.
Anesthetized guinea-pigs were intravenously injected with Evans blue. After intracutaneous injection of agonists (lys-plasminogen, histamine, platelet-activating factor, thrombin, bradykinin), the resulting wheals appeared blue in a dose-dependent manner, due to an enhanced capillary permeability, alpha 1-Acid glycoprotein, given i.v. in different doses (3.125-50 mg/kg) and at different times (30-180 min) before Evans blue administration, antagonized the effects of all agonists listed above. This was shown by a parallel shift of the agonist dose-response curves to the right. The effect was time-dependent (tmax: mainly 120 min) and dose-dependent. alpha 1-Acid glycoprotein antagonized the agonists in the following order: lys-plasminogen > histamine = platelet-activating factor > thrombin > bradykinin. As all agonist mentioned are suggested to play a major role in the shock-related increase in vascular permeability, a putatively beneficial role of alpha1-acid glycoprotein in shock is discussed.
Muchitsch
E M
EM
Immuno AG, Vienna, Austria.
Teschner
W
W
Linnau
Y
Y
Pichler
L
L
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D005070:Evans Blue; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D008297:Male; D009961:Orosomucoid / Q000494:pharmacology*; D010958:Plasminogen / Q000494:pharmacology; D012867:Skin / Q000098:blood supply* / Q000187:drug effects
D009961:Orosomucoid; D005070:Evans Blue; D006632:Histamine; D010958:Plasminogen