Anti-single-stranded DNA antibody in the sera of patients with type 2 diabetes mellitus. Relation to vascular complications.
Anti-single-stranded(ss)DNA antibodies were searched for by enzyme-linked immunosorbent assay (ELISA) in the serum of 202 outpatients with non-insulin-dependent diabetes mellitus and 135 healthy subjects to investigate their prevalence in the serum of patients with type 2 diabetes and their relationship with the presence of vascular complications. Of the 202 patients 128 had vascular complications. Anti-ssDNA antibodies were observed to be significantly more frequent in the serum of patients with vascular complications (33.6%) and in particular in patients with overt nephropathy (50%) than in patients without complications (6.7%) or controls (6.7%). Anti-ssDNA antibodies have been previously described in patients with type 1 diabetes before clinical evidence of vascular disease and their cross-reactivity with a variety of anionic biological molecules or cells, i.e. platelets and endothelial cells, assessed. It seems not unreasonable that these auto-antibodies detected in patients with type 2 diabetes could be of importance in the pathogenesis or progression of angiopathy.
Giardina
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E
Cattedra di Immunologia Clinica e Allergologia, Istituto di Clinica Medica, Palermo, Italy.
Triolo
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G
Accardo-Palumbo
A
A
Carbone
M C
MC
Gancitano
V
V
Verga
S
S
Triolo
G
G
D000974:Antibodies, Antinuclear / Q000097:blood*; D001786:Blood Glucose / Q000032:analysis; D004277:DNA, Single-Stranded / Q000276:immunology*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000276:immunology*; D003925:Diabetic Angiopathies / Q000453:epidemiology / Q000276:immunology; D003928:Diabetic Nephropathies / Q000453:epidemiology / Q000276:immunology; D003930:Diabetic Retinopathy / Q000453:epidemiology / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012016:Reference Values
D000974:Antibodies, Antinuclear; D001786:Blood Glucose; D004277:DNA, Single-Stranded