Serial cytokine changes in peritoneal effluent and plasma during peritonitis in patients on continuous ambulatory peritoneal dialysis (CAPD).
OBJECTIVE
Secretion of IL-2 and sIL-2R in the peritoneal dialysis fluid and in the peripheral blood during peritonitis with reference to the process of IL-6 and IL-8 release were investigated.


METHODS
17 patients with end-stage renal disease, treated with continuous ambulatory peritoneal dialysis.


METHODS
ELISA method using commercial kits, Genzyme Corp Boston and DAKO.


RESULTS
Markedly increased IL-6 (mean; 2895 +/- 1360 pg/ml) and IL-8 concentration (1459 +/- 966 pg/ml) in drain dialysate fluid at the onset of peritonitis, started to drop rapidly in the successive samples after antibiotics had been administered. Statistically significant increase of IL-2 (197 +/- 92 pg/ml) and sIL-2R (287 +/- 79 pg/ml) was observed 16 h later and kept increasing until reaching the peak after 24 h.


CONCLUSIONS
Secretion of IL-6 and IL-8 pro-inflammatory cytokines which are mainly synthesized in mesothelium cells is followed by the activation of lymphocytes, their infiltration and the production of T lymphocyte derived IL-2 and sIL-2R.
Kozioł-Montewka
M
M
Nephrology Department, Medical Academy, Lublin, Poland.
Ksiazek
A
A
Janicka
L
L
Baranowicz
I
I
D000328:Adult; D000368:Aged; D001202:Ascitic Fluid / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000097:blood* / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood* / Q000378:metabolism; D016209:Interleukin-8 / Q000097:blood* / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000628:therapy; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects*; D010538:Peritonitis / Q000097:blood / Q000209:etiology / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000097:blood* / Q000378:metabolism; D012044:Regression Analysis
D007376:Interleukin-2; D015850:Interleukin-6; D016209:Interleukin-8; D015375:Receptors, Interleukin-2