Distribution pattern of nervous tissue and peptidergic nerve fibers in accessory lacrimal glands.
OBJECTIVE
Demonstration of the distribution pattern of nervous tissue and the occurrence of neuropept human accessory lacrimal glands by means of immunohistochemical methods.


METHODS
Paraffin sections of tissue samples were incubated with antisera against protein gene product (PGP), S-100 protein (S-100), calcitonin gene-related peptide (CGRP) and substance P (SP). The immunoreactions were visualized using both species-specific secondary antibodies and a streptavidin-biotin-peroxidase complex (ABC method).


RESULTS
PGP- and S-100-immunoreactive nerve fibers were distributed in the entire intertubular stroma. In addition, myoepithelial cells, a few clusters of tubulus cells, groups of wandering cells, and scattered cells of the excretory duct system were S-100-immunoreactive. CGRP- and SP-immunoreactivity was found in a few nerve fibers in the intertubular glandular stroma with an association to secretory tubules, blood vessels, intralobular ducts and excretory duct.


CONCLUSIONS
These observations are in agreement with findings in the main lacrimal gland.
Seifert
P
P
Alfried-Krupp-Laboratory, Department of Ophthalmology, University of Bonn, Germany.
Stuppi
S
S
Spitznas
M
M
D000328:Adult; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007765:Lacrimal Apparatus / Q000033:anatomy & histology* / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009412:Nerve Fibers / Q000378:metabolism* / Q000648:ultrastructure*; D009417:Nerve Tissue / Q000033:anatomy & histology*; D009479:Neuropeptides / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism
D009479:Neuropeptides; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide