Enantioselective determination of diprafenone in human plasma.
A sensitive (10.0 ng/ml) and stereoselective HPLC method for analysis of S(-)- and R(+)-diprafenone in human plasma has been developed. The assay is based on derivatization with homochiral R(-)-1-(1-naphthyl)ethyl isocyanate to give the diastereomeric derivates and their separation and quantitation using HPLC with UV-detection (220 nm). Details of this stereoselective assay procedure which has already been applied to the multisample analyses from a human pharmacokinetic study are described. The data obtained indicated that the stereoselective disposition of diprafenone enantiomers is quite different from their structural and pharmacological analog propafenone.
Zhong
D
D
Department of Pharmacy, Shenyang Pharmaceutical University, P.R. China.
Chen
R
R
Fieger-Büschges
H
H
Blume
H
H
D000284:Administration, Oral; D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007275:Injections, Intravenous; D011405:Propafenone / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000889:Anti-Arrhythmia Agents; C047546:diprafenone; D011405:Propafenone