Glued participates in distinct microtubule-based activities in Drosophila eye development.
A C-terminal truncation of Glued, the Drosophila homolog of the cytoplasmic dynein activating protein, dynactin, results in a severe and complex retinal phenotype, including a roughening of the facet array, malformation of the photosensitive rhabdomeres, and a general deficit and disorder of retinal cells. We have characterized the developmental phenotype in Glued1 and found defects in multiple stages of eye development, including mitosis, nuclear migration, cell fate determination, rhabdomere morphogenesis and cell death. Transgenic flies that express dominant negative Glued under heat-shock control reproduce distinct features of the original Glued1 phenotype depending on the stage of development. The multiple phenotypes effected by truncated Glued point to the multiple roles served by dynactin/dynein during eye development.
Fan
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Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
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D000818:Animals; D030801:Animals, Genetically Modified; D016923:Cell Death; D002465:Cell Movement; D002467:Cell Nucleus / Q000648:ultrastructure; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology*; D000072159:Dynactin Complex; D004398:Dyneins / Q000502:physiology*; D005123:Eye / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D005799:Genes, Dominant; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008870:Microtubules / Q000502:physiology*; D008938:Mitosis; D009024:Morphogenesis; D012160:Retina / Q000648:ultrastructure; D017384:Sequence Deletion; D013329:Structure-Activity Relationship
C105393:DCTN1-p150 protein, Drosophila; D029721:Drosophila Proteins; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D004398:Dyneins