title: Cooling strangulated intestine before and following release of an obstruction protects from ischemia/reperfusion injury.
abstract: OBJECTIVE
  To evaluate whether cooling of ischemic intestine before and immediately after the release of the obstruction is more advantageous than is warming in an isolated ileal loop rat model.
  
  
  METHODS
  Prospective randomized experimental trials.
  
  
  METHODS
  Laboratory.
  
  
  METHODS
  Male Sabra rats.
  
  
  METHODS
  An isolated loop of ileum was created in rats and constantly perfused with warmed normal saline (37 degrees C). Intestinal ischemia was caused by clamping of the superior mesenteric artery for 15 minutes. Cooling of the isolated ileal loop to 32 degrees C by the application of cold saline was done for the last half of the ischemic period and continued during the first 10 minutes of reperfusion. The intestine was warmed to 37 degrees C for the next 20 minutes of reperfusion.
  
  
  METHODS
  The chromium 51-labeled ethylenediaminetetraacetic acid (51Cr-EDTA) plasma-to-lumen clearance rate was used for the evaluation of intestinal mucosal injury. Multiple intestinal samples were harvested at the end of the reperfusion period, and the histological features were evaluated.
  
  
  RESULTS
  The 51Cr-EDTA plasma-to-lumen clearance rates were significantly lower (P < .001) in the rats in which cooling of the ischemic intestine was performed in comparison with the control group, indicating reduced intestinal mucosal injury in all three 10-minute reperfusion periods. Cooling also significantly changed the resultant histological damage to the intestinal mucosa exerted by ischemia (P < .001).
  
  
  CONCLUSIONS
  Cooling of the ischemic intestine before the release of the obstruction and for a short period during reperfusion provides significant protection from ischemia/ reperfusion injury compared with the traditional method of warming the ischemic intestine.
authors.affiliation: Department of General and Pediatric Surgery, Hadassah University Hospital Mount Scopus, Jerusalem, Israel. udassin@cc.huji.ac.il
authors.forename: R
authors.initials: R
authors.lastname: Udassin
authors.forename: Y
authors.initials: Y
authors.lastname: Haskel
authors.forename: D
authors.initials: D
authors.lastname: Seror
mesh_terms: D000818:Animals; D007415:Intestinal Obstruction / Q000150:complications*; D008297:Male; D011897:Random Allocation; D051381:Rats; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D013696:Temperature; D013997:Time Factors