Keratinocyte growth factor increases surfactant pool sizes in premature rabbits.
Keratinocyte growth factor (KGF) is a recently described 19-kD glycoprotein that is a mitogen for epithelial-derived tissue and is expressed during embryonic tissue differentiation. We investigated whether human recombinant KGF (rKGF) could increase alveolar and lung-tissue saturated phosphatidyl-choline (Sat PC) in preterm rabbits delivered at 28.5 d gestation. Preterm rabbits were given 1, 5, or 10 mg/kg rKGF by tracheal injection at 30 min of age. Lung tissue and total lung (alveolar plus lung tissue) Sat PC increased by 48 h (5 and 10 mg/kg) (p < 0.01) and 72 h (1 and 10 mg/kg) (p < 0.05). A single intravascular or intramuscular dose of 10 mg/kg rKGF also increased long tissue and total-lung Sat PC by 48 h (p < 0.01). A single dose of rKGF given shortly after birth to preterm rabbits increased lung Sat PC in the preterm rabbits.
Ikegami
M
M
Department of Pediatrics, Harbor-UCLA Medical Center, UCLA School of Medicine, Torrance 90502, USA.
Jobe
A H
AH
Havill
A M
AM
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D004848:Epithelium / Q000187:drug effects; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000494:pharmacology* / Q000627:therapeutic use; D008168:Lung / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D010713:Phosphatidylcholines / Q000032:analysis*; D011663:Pulmonary Surfactants / Q000032:analysis*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use
D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; D011994:Recombinant Proteins; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors