Binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin to AH receptor in placentas from normal versus abnormal pregnancy outcomes.
Binding of [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin to AH receptor was characterized in cytosol from human placentas in which the pregnancy outcome was normal compared with pregnancies in which there was some adverse outcome (premature birth; intrauterine growth retardation; structural abnormality). No significant difference was detected between normal and adverse outcomes in the concentration of AH receptor sites (Bmax) nor in the affinity with which [3H]TCDD bound to the receptor (Kd). Aryl hydrocarbon hydroxylase activity, a CYP1A1 enzyme regulated by the AH receptor, was elevated in placental microsomes from smokers; this elevation was associated with intrauterine growth retardation.
Okey
A B
AB
Department of Pharmacology, University of Toronto, Ontario, Canada.
Giannone
J V
JV
Smart
W
W
Wong
J M
JM
Manchester
D K
DK
Parker
N B
NB
Feeley
M M
MM
Grant
D L
DL
Gilman
A
A
D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D000013:Congenital Abnormalities / Q000209:etiology / Q000378:metabolism; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology / Q000378:metabolism; D006801:Humans; D007752:Obstetric Labor, Premature / Q000209:etiology / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D011248:Pregnancy Complications / Q000209:etiology / Q000378:metabolism*; D011256:Pregnancy Outcome; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D001189:Aryl Hydrocarbon Hydroxylases