title: Nucleotide excision repair capacity is attenuated in human promyelocytic HL60 cells that overexpress BCL2.
abstract: We investigated the effect of the BCL2 overexpression on nucleotide excision repair (NER) and DNA replication in UV-irradiated HL60 cells. Forty-eight h after 10 J/m2 irradiation, only 4% of the cyclobutane pyrimidine dimers were removed in the BCL2-overexpressing cells, in contrast to 38% removal in control cells. However, the repair of 6-4 pyrimidine pyrimidone photoproducts was not affected by BCL2 overexpression. Eight h after irradiation, DNA replication recovered to 60% of normal in the BCL2-overexpressing cells, whereas little DNA replication recovered in control cells. The antioxidant N-acetyl cysteine also attenuated cyclobutane pyrimidine dimer removal but did not enhance the recovery of DNA replication. Both BCL2-overexpressing and NAC-treated cells were more resistant to UV. Our data suggest that Bcl2 may promote mutagenesis and genomic instability in surviving cells.
authors.affiliation: Department of Biological Sciences, Stanford University, California 94305-5020, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Naumovski
authors.forename: P
authors.initials: P
authors.lastname: Hanawalt
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D017209:Apoptosis; D002470:Cell Survival / Q000528:radiation effects; D053938:DNA Fragmentation; D004260:DNA Repair; D004261:DNA Replication / Q000528:radiation effects; D018922:HL-60 Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000502:physiology*; D011740:Pyrimidine Dimers; D011994:Recombinant Proteins; D014466:Ultraviolet Rays
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D011740:Pyrimidine Dimers; D011994:Recombinant Proteins; D000111:Acetylcysteine