title: Isolation and amino acid sequence of a new long-chain neurotoxin with two chromatographic isoforms (Aa el and Ae e2) from the venom of the Australian death adder (Acanthophis antarcticus).
abstract: The amino acid sequence of a previously undescribed toxin from Australian death adder venom (Acanthophis antarcticus) has been elucidated. It appears to exist in two forms which are separated by reverse-phase high-performance liquid chromatography, but which have the same sequence and mol. wt. It has 79 amino acid residues and is therefore longer than other long postsynaptic neurotoxins. It shows homology with the conserved regions of the other long postsynaptic neurotoxins except for three unique substitutions of conserved residues, which are Arg-29 instead of Trp or Phe, Leu-33 instead of Arg and Thr-43 instead of Ala.
authors.affiliation: Deakin Research Ltd, c/-CSIRO, Division of Food Science and Technology, NSW, Australia.
authors.forename: M I
authors.initials: MI
authors.lastname: Tyler
authors.forename: K V
authors.initials: KV
authors.lastname: Retson-Yip
authors.forename: M K
authors.initials: MK
authors.lastname: Gibson
authors.forename: D
authors.initials: D
authors.lastname: Barnett
authors.forename: E
authors.initials: E
authors.lastname: Howe
authors.forename: R
authors.initials: R
authors.lastname: Stöcklin
authors.forename: R K
authors.initials: RK
authors.lastname: Turnbull
authors.forename: T
authors.initials: T
authors.lastname: Kuchel
authors.forename: P
authors.initials: P
authors.lastname: Mirtschin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001315:Australia; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004546:Elapid Venoms / Q000737:chemistry* / Q000302:isolation & purification*; D017815:Elapidae; D051379:Mice; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry* / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D004546:Elapid Venoms; D009498:Neurotoxins; C106943:toxin Aa e, Acanthophis