[Chiral separation of seven beta-blockers in the French pharmacopoeia].
Determination of optical isomers is based on some molecule properties to have one or more chiral asymmetrical carbon center. Enantiomers can be resolved by high performance liquid chromatography (HPLC) with a chiral mobile phase additive, by change into diastereoisomers by derivatization or by separation on chiral column. We retained the last technique for the separation of seven beta-blockers (atenolol, alprenolol, labetalol, nadolol, oxprenolol, pindolol and propranolol) using an alpha 1-AGP column. Results indicate that the mobile phase was specific to each beta-blocker. The wavelength chosen was lambda = 225 nm and the flow rate was 0.9 ml/min. A part from that, determinations of isomers of propranolol and atenolol in different fluids biologicals were also achieved.
Berthault
F
F
Institut de Médecine Légale, Strasbourg.
Kintz
P
P
Mangin
P
P
D000319:Adrenergic beta-Antagonists / Q000737:chemistry* / Q000302:isolation & purification; D000328:Adult; D001262:Atenolol / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005602:France; D006801:Humans; D008297:Male; D010602:Pharmacopoeias as Topic; D011433:Propranolol / Q000032:analysis / Q000737:chemistry; D013237:Stereoisomerism
D000319:Adrenergic beta-Antagonists; D001262:Atenolol; D011433:Propranolol