Gestational age and cell viability determine the effect of frozen storage on human fetal hematopoietic progenitor cell preparations.
We have assessed hematopoietic progenitor cell preparations (including CD34-positive stem cells) obtained from human fetal liver at 6-17 weeks of gestational age for total cell numbers, viability and the ability to tolerate (frozen) storage. Hematopoietic cell preparations obtained from the 16-to 17-week gestational age range had the highest total cell count and the greatest proportion of CD34-positive stem cells. However, these preparations were insufficiently robust to withstand the freeze/thaw protocol required. Cells obtained from the 13- to 15-week gestational age range showed optimal post-thaw viability and it is suggested that these cell preparations are the most applicable for in utero transplantation.
Anderson
E M
EM
Department of Immunology, Queens Medical Centre, Nottingham, UK.
Jones
D R
DR
Liu
D T
DT
Evans
A A
AA
D000021:Abortifacient Agents, Steroidal; D000028:Abortion, Induced; D018952:Antigens, CD34 / Q000032:analysis; D002452:Cell Count; D002470:Cell Survival; D015925:Cryopreservation; D005260:Female; D005865:Gestational Age; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000196:embryology*; D015735:Mifepristone; D011247:Pregnancy
D000021:Abortifacient Agents, Steroidal; D018952:Antigens, CD34; D015735:Mifepristone