Insulin/glucose-, pyruvate- and polyunsaturated fatty acid-responsive region(s) of rat fatty acid synthase gene promoter.
To investigate the regulatory DNA sequences required for polyunsaturated fatty acid (PUFA)-suppression of fatty acid synthase (FAS) gene as well as for insulin and/or carbohydrate-stimulation of this gene, primary hepatocytes were transfected with plasmids containing the 5'-flanking sequence of the rat FAS gene fused to the CAT gene. Sequences from -1604, -88 or -57 to +79 of the FAS gene directed an increase in CAT activity in the hepatocytes when insulin/glucose was added to the medium, in accordance with the responses on the endogenous FAS gene expression. The CAT activities were reduced by the addition of PUFA. Further deletion to -34, however, resulted in loss of the responses. The results suggest that the region from -57 to -34 of the FAS gene may be responsible for regulation due to insulin/glucose and PUFAs. Moreover, the region was also responsible for stimulation due to pyruvate alone.
Fukuda
H
H
Tezukayama Gakuin College, Sakai, Japan.
Iritani
N
N
Katsurada
A
A
Noguchi
T
T
D000818:Animals; D064429:Fatty Acid Synthases / Q000235:genetics*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D019289:Pyruvic Acid / Q000494:pharmacology*; D051381:Rats
D005231:Fatty Acids, Unsaturated; D007328:Insulin; D019289:Pyruvic Acid; D064429:Fatty Acid Synthases; D005947:Glucose