Phenylguanine found in urine after benzene exposure.
Comparative investigations with synthetic N7-phenylguanine were carried out to clarify whether this compound is eliminated via the urine of rats as a benzene-derived nucleic acid adduct. As sensitive methods for detecting trace amounts of the compound, gas chromatography-mass spectroscopy, high performance liquid chromatography, and two immunoassays (enzyme-linked immunosorbent assay and fluoroimmunoassay) with appropriate monoclonal antibodies were used. The results indicate the excretion of several benzene-related guanine adducts slightly different from N7-phenylguanine that may possibly be hydroxylated. These adducts differ also from (O6-, N2- and C8-phenylguanine, respectively.
Norpoth
K H
KH
Institute of Hygiene and Occupational Medicine, University Medical Center, Essen, Germany. tha020@sp2.power.uni-essen.de
Müller
G
G
Schell
C
C
Jorg
E
E
D000818:Animals; D000911:Antibodies, Monoclonal; D001554:Benzene / Q000493:pharmacokinetics* / Q000633:toxicity*; D001711:Biotransformation; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D018736:DNA Adducts / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006147:Guanine / Q000031:analogs & derivatives* / Q000276:immunology / Q000652:urine; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D002250:Carbon Radioisotopes; D018736:DNA Adducts; C056235:N(7)-phenylguanine; D006147:Guanine; D001554:Benzene