POU-domain factor expression in the trigeminal ganglion and implications for herpes virus regulation.
The restriction of herpes virus latency to mammalian sensory ganglia has led to a search for tissue-specific regulatory molecules in these neurons which alter viral gene expression. Based primarily on work in sensory-related cell lines, several prior reports have suggested that the POU-domain transcription factor Oct-2 functions in the establishment of viral latency. Here we show that among the POU-domain transcriptional regulators, Brn-3.0 is abundantly expressed in the postnatal trigeminal ganglion. In contrast, Oct-2 message and protein are not detectable by sensitive and specific assays, effectively excluding a direct role for Oct-2 in the viral life cycle in vivo.
Turner
E E
EE
Department of Psychiatry, University of California, School of Medicine, La Jolla 92093-0603, USA.
Fedtsova
N
N
Rosenfeld
M G
MG
D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D006564:Herpesviridae / Q000235:genetics*; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D050813:Octamer Transcription Factor-2; D050809:POU Domain Factors; D012333:RNA, Messenger / Q000096:biosynthesis; D012260:Ribonucleases / Q000378:metabolism; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D012668:Trigeminal Ganglion / Q000378:metabolism*
D004268:DNA-Binding Proteins; D050813:Octamer Transcription Factor-2; D050809:POU Domain Factors; C489206:Pou2f2 protein, mouse; C494189:Pou4f1 protein, mouse; D012333:RNA, Messenger; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors; D012260:Ribonucleases