Tissue distribution of DNA adducts and their persistence in blood of mice exposed to benzene.
Chemicals combine with DNA, resulting in DNA damage, which could initiate carcinogenesis. To study whether benzene or benzene metabolites bind to DNA, DNA adducts in various tissues and their persistence in leukocytes were examined using the 32P-postlabeling assay. LACA mice were dosed ip with benzene at 500 mg/kg bw twice for 5 days. Two additional spots of DNA adducts are formed in bone marrow cells, liver cells, and peripheral blood compared with control mice. The relative adduct labeling values are 10.39, 11.32, and 13.77 adducts x 10(-8) nucleotides in these tissues, respectively. DNA adducts in blood leukocytes were observed at 1, 4, 7, 14, and 21 days after exposure to benzene, but adduct levels decreased as a function of time. Relative adduct labeling of "adduct B" declined linearly but mildly, while "adduct C" displayed a stepwise decrease. The relative adduct labeling values of both these adducts at day 14 were 50% of those at day 1 after the last treatment. Both adducts were still detectable at day 21 after benzene exposure. These studies demonstrate that benzene could induce DNA adducts in bone marrow, liver, and white blood cells of mice dosed with benzene and that measurement of adducts in white blood cells may be useful as a biomarker to predict carcinogenic risk of benzene to workers exposed to benzene.
Li
G
G
Institute of Occupational Medicine Chinese Academy of Preventive Medicine, Beijing, China, USA.
Wang
C
C
Xin
W
W
Yin
S
S
D000818:Animals; D001554:Benzene / Q000378:metabolism / Q000633:toxicity*; D015415:Biomarkers / Q000097:blood; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D018736:DNA Adducts / Q000097:blood* / Q000378:metabolism*; D004249:DNA Damage; D004784:Environmental Monitoring; D005260:Female; D007700:Kinetics; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D014018:Tissue Distribution
D015415:Biomarkers; D018736:DNA Adducts; D004247:DNA; D001554:Benzene