Towards amperometric immunosensor devices.
In contrast to optical immunosensors, the electrochemical detection of an immunanalytical reaction does require a labeling, but allows an easier discrimination of specific and non-specific binding. We present a concept and first results for a multivalent amperometric immunosensor system which is based on silicon technology. The capture molecule streptavidin, covalently immobilized on silica, allows the immobilization of biotinylated antigens at a defined density. A nanostructured gold electrode serving as a stable network of nanowires is expected to be beneficial for the electrochemical detection of bound ferrocene-labeled antibody molecules. The results presented focus on site-specific immobilization of streptavidin on silica and reduction of non-specific binding of proteins.
Tiefenauer
L X
LX
Paul Scherrer Institut, Micro- and Nanostructures Laboratory, Villigen, Switzerland. tiefenauer@psi.ch
Kossek
S
S
Padeste
C
C
Thiébaud
P
P
D001426:Bacterial Proteins; D015374:Biosensing Techniques; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006046:Gold; D012822:Silicon Dioxide; D019809:Streptavidin
D001426:Bacterial Proteins; D006046:Gold; D012822:Silicon Dioxide; D019809:Streptavidin