title: Simian T-lymphotropic virus type 1 (STLV-1) infection in wild yellow baboons (Papio hamadryas cynocephalus) from Mikumi National Park, Tanzania.
abstract: Serum and peripheral blood leukocytes from wild yellow baboons (Papio hamadryas cynocephalus) were tested for the presence of STLV-1-specific antibodies and proviral DNA. Fourteen of 30 sera tested positive by radioimmunoprecipitation assay (RIPA) with HTLV-1. Among 36 DNA samples tested by PCR 15 were positive by double nested PCR for a fragment of the STLV-1 env gene, the most sensitive assay among PCR tests employed. Of 30 animals that were tested both serologically and by PCR in only 1 case were the results discordant (PCR-positive, antibody-negative). The DNA sequences from env (378 bp), pol (212 bp), and LTR (705 bp) were determined for 5, 5, and 2 Mikumi STLV-1 isolates, respectively. The DNA sequences of Mikumi STLV-1 isolates were virtually identical and phylogenetic analysis revealed that they were clearly distinct from previously published baboon STLV-1 sequences, including those STLV-1 isolates presumed to be from yellow baboons. The results of this study suggest that reliable placement of individual STLV-1 within the PTLV-1 phylogeny requires genomic sequences of STLV-1 isolates from wild animals whose taxonomic identity and geographical origin are firmly established and that the LTR is the genomic region of STLV-1 which is the most informative for cladistic analysis of these viruses.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait. voevodin@hscc.kuniv.edu.kw
authors.forename: A
authors.initials: A
authors.lastname: Voevodin
authors.forename: E
authors.initials: E
authors.lastname: Samilchuk
authors.forename: J
authors.initials: J
authors.lastname: Allan
authors.forename: J
authors.initials: J
authors.lastname: Rogers
authors.forename: S
authors.initials: S
authors.lastname: Broussard
mesh_terms: D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000097:blood; D015480:Deltaretrovirus Antibodies / Q000097:blood; D006800:Deltaretrovirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D015686:Gene Products, env / Q000235:genetics; D015684:Gene Products, pol / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology*; D010215:Papio; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D015303:Simian T-lymphotropic virus 1 / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013636:Tanzania; D014407:Tumor Cells, Cultured
chemical_list: D004279:DNA, Viral; D015480:Deltaretrovirus Antibodies; D015686:Gene Products, env; D015684:Gene Products, pol