Selection of biochemical mutants of Aspergillus niger with enhanced catalase production.
The production of extracellular catalase in a submerged culture by a number of biochemical mutants has been evaluated. Eight of these mutants showed increased extracellular catalase, the level of which ranged widely in individual cases from 44% to over 94% in comparison with the parental strain. Studies of the relationship between a criterion of selection and the frequency of mutation showed that the highest frequency of positive mutations (15.8% and 24.2%) was obtained with respect to mutants resistant to ethidium bromide (1 mmol/l) and sodium gluconate (45%) respectively. The time course of growth and enzyme production by the most active mutant, AM-20, showed extra- and intracellular catalase activities increasing about 2- and 2.6-fold respectively, compared with the parental strain.
Fiedurek
J
J
Department of Industrial Microbiology, Maria Curie-Sklodowska University, Lublin, Poland.
Gromada
A
A
D001234:Aspergillus niger / Q000201:enzymology*; D002374:Catalase / Q000096:biosynthesis*; D005949:Glucose Oxidase / Q000378:metabolism; D009154:Mutation
D005949:Glucose Oxidase; D002374:Catalase