Androgen regulation of ornithine decarboxylase in human prostatic cells identified using differential display.
Androgens are essential for normal prostate physiology and have a permissive role in the development and progression of prostate cancer. Using the mRNA differential display technique, ornithine decarboxylase (ODC) was identified to be up-regulated by androgens in human prostatic LNCaP cells. On Northern analysis, the induction of ODC expression by 10 nM androgen was rapid and continued up to 48 h exposure with a maximum 6.3-fold up-regulation. The anti-androgen Casodex inhibited the androgen-induced up-regulation of ODC, whereas the protein synthesis inhibitor cycloheximide did not. Together these data suggest that regulation is mediated through the androgen receptor protein and does require secondary protein synthesis, respectively. The kinetics of induction of ODC were almost identical to those of prostate specific antigen. Taken together these data suggest that ODC is directly regulated by androgens in LNCaP cells.
Betts
A M
AM
Department of Surgery, University of Newcastle, Newcastle upon Tyne, UK.
Waite
I
I
Neal
D E
DE
Robson
C N
CN
D001483:Base Sequence; D003513:Cycloheximide / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000235:genetics*; D011467:Prostate / Q000201:enzymology*; D017430:Prostate-Specific Antigen / Q000235:genetics; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D045165:Testosterone Congeners / Q000494:pharmacology*; D013997:Time Factors
D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D045165:Testosterone Congeners; D009277:Nandrolone; D003513:Cycloheximide; C100075:mibolerone; D017430:Prostate-Specific Antigen; D009955:Ornithine Decarboxylase