Characterization of an interleukin 1 receptor antagonist protein released from ultraviolet-irradiated epidermal sheet.
An interleukin 1 (IL-1) inhibitor was detected in the culture supernatant of mouse epidermal sheet which had been irradiated with middle-wavelength ultraviolet (UVB) either in vitro or in vivo. Properties of the IL-1 inhibitor were consistent with those of IL-1 receptor antagonist (IL-1ra), i.e., approximate molecular size of 17 kDa by gel filtration, specific inhibition of IL-1-induced thymocyte proliferation, inhibition of binding of IL-1 to IL-1 receptor on the T-cell surface, and reactivity to anti-mouse IL-1ra antibody shown by immunoblotting and enzyme-linked immunosorbent assay (ELISA). These results indicate that this IL-1 inhibitor, which appears to be identical with IL-1ra, was released from the epidermis upon UVB irradiation, and that the inhibitor may participate in the regulation of inflammation mediated by IL-1, as well as in the pathogenesis of ultraviolet (UV)-induced immunosuppression.
Hirao
T
T
Institute for Advanced Skin Research Inc., Yokohama, Japan.
Aoki
H
H
Ichikawa
H
H
Sato
Y
Y
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D004817:Epidermis / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects*; D015151:Immunoblotting; D007165:Immunosuppression Therapy; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012795:Sialoglycoproteins / Q000032:analysis* / Q000378:metabolism* / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology; D013997:Time Factors; D014466:Ultraviolet Rays
C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D007457:Iodine Radioisotopes; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins