IFN-gamma induces expression of MHC class I molecules in adult mouse dorsal root ganglion neurones.
The expression of major histocompatibility complex (MHC) class I molecules on adult mouse DRG neurones was analysed using immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR). MHC class I molecules were not expressed in untreated dorsal root ganglion (DRG) neurones. The molecules, however, were detected on the surface of neurones following IFN-gamma treatment. In addition, expression of the MHC class I beta 2-microglobulin and genes was induced in neurones following IFN-gamma treatment. Expression of Tap-1 and Lmp-2 genes, which are involved in antigen processing and presentation, was also induced by IFN-gamma treatment. These results indicated that DRG neurones are able to express MHC class I molecules following IFN-gamma treatment and suggest that DRG neurones themselves are susceptible to recognition by cytotoxic T cells.
Fujimaki
H
H
Department of Immunology and Parasitology, School of Medicine, Yokohama City University, Japan.
Hikawa
N
N
Nagoya
M
M
Nagata
T
T
Minami
M
M
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000096:biosynthesis; D019085:Fluorescent Antibody Technique, Direct; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005805:Genes, MHC Class I / Q000187:drug effects*; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D001613:beta 2-Microglobulin / Q000378:metabolism
D001613:beta 2-Microglobulin; D007371:Interferon-gamma; D004247:DNA