Clean-up of aqueous acetone vegetable extracts by solid-matrix partition for pyrethroid residue determination by gas chromatography-electron-capture detection.
Disposable, ready-to-use cartridges filled with macroporous diatomaceous material are used to carry out a partition clean-up that, in a single step, is capable of transferring pesticide residues from aqueous acetone extracts into light petroleum-dichloromethane (75:25, v/v). This procedure takes the place of some functions (such as separatory-funnel partition, drying over anhydrous sodium sulphate and partial adsorption clean-up) usually performed by separate steps in classical schemes. Fourteen pyrethroid pesticides, including tefluthrin, tetramethrin, cyphenothrin, cyfluthrin, flucythrinate, tau-fluvalinate, deltamethrin, bioallethrin, fenpropathrin, lambda-cyhalothrin, permethrin, alpha-cypermethrin, esfenvalerate and tralomethrin were determined using the described procedure with satisfactory recoveries for most of them, at spiking levels ranging from 0.08 to 0.82 mg/kg for the different compounds. Crops subjected to the described procedure included strawberry, apple, and orange gave extracts containing a mass of co-extractives that was between 5 and 30 mg. Compared with classical schemes, the described procedure is simple, less labour intensive, allows parallel handling of several extracts and does not require the preparation and maintenance of equipment. Troublesome emulsions such as those frequently observed in separation funnel partitioning do not occur.
Di Muccio
A
A
ISS-Istituto Superiore di Sanità (National Institute of Health), Lab. Tossicologia Applicata, Rome, Italy.
Barbini
D A
DA
Generali
T
T
Pelosi
P
P
Ausili
A
A
Vergori
F
F
Camoni
I
I
D000096:Acetone; D002849:Chromatography, Gas / Q000379:methods*; D007306:Insecticides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*; D014867:Water
D007306:Insecticides; D010573:Pesticide Residues; D010936:Plant Extracts; D011722:Pyrethrins; D014867:Water; D000096:Acetone