Vasopressin synergistically stimulates DNA synthesis in normal and regenerating rat liver cell cultures in the presence of hepatocyte growth factor.
Liver regeneration is significantly impaired in rats with both alpha-adrenergic hepatic denervation and hereditary vasopressin deficiency. This may implicate a direct role for these agonists in the process of compensatory hyperplasia. The mitogenic capacities of norepinephrine, vasopressin and hepatocyte growth factor (HGF), either alone or in combination were investigated by [3H]thymidine incorporation into hepatocyte cultures prepared from normal and regenerating rat livers. The results show that normal hepatocytes incorporate less [3H]thymidine in response to HGF than do regenerating hepatocytes. In addition, physiological concentrations of vasopressin cause a synergistic stimulation of [3H]thymidine uptake in rat liver cells in the presence of HGF.
Metcalfe
A M
AM
MRC Clinical and Biochemical Resonance Unit, Department of Biochemistry, University of Oxford, UK.
Phillips
P
P
Dixon
R M
RM
Radda
G K
GK
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D017228:Hepatocyte Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008115:Liver Regeneration / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013936:Thymidine / Q000378:metabolism; D014667:Vasopressins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*
D014667:Vasopressins; D017228:Hepatocyte Growth Factor; D004247:DNA; D013936:Thymidine; D009638:Norepinephrine