Validation of a simple, manual urinary iodine method for estimating the prevalence of iodine-deficiency disorders, and interlaboratory comparison with other methods.
The measurement of urinary iodine in population-based surveys provides a biological indicator of the severity of iodine-deficiency disorders. We describe the steps performed to validate a simple, inexpensive, manual urinary iodine acid digestion method, and compare the results using this method with those of other urinary iodine methods. Initially, basic performance characteristics were evaluated: the average recovery of added iodine was 100.4 +/- 8.7% (mean +/- SD), within-assay precision (CV) over the assay range 0-0.95 mumol/L (0-12 micrograms/dL) was < 6%, between-assay precision over the same range was < 12%, and assay sensitivity was 0.05 mumol/L (0.6 microgram/dL). There were no apparent effects on the method by thiocyanate, a known interfering substance. In a comparison with five other methods performed in four different laboratories, samples were collected to test the method performance over a wide range of urinary iodine values (0.04-3.7 mumol/L, or 0.5-47 micrograms/dL). There was a high correlation between all methods and the interpretation of the results was consistent. We conclude that the simple, manual acid digestion method is suitable for urinary iodine analysis.
May
S L
SL
Department of International Health, Rollins School of Public Health of Emory University, Atlanta, GA, USA. SLM4@CEHEHL1.EM.CDC.GOV
May
W A
WA
Bourdoux
P P
PP
Pino
S
S
Sullivan
K M
KM
Maberly
G F
GF
D001151:Arsenic / Q000737:chemistry; D002563:Cerium / Q000737:chemistry; D002624:Chemistry, Clinical / Q000379:methods*; D002704:Chlorates; D006801:Humans; D007455:Iodine / Q000172:deficiency* / Q000652:urine*; D007753:Laboratories; D010084:Oxidation-Reduction; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013861:Thiocyanates / Q000652:urine
D002704:Chlorates; D013861:Thiocyanates; D002563:Cerium; D007455:Iodine; D001151:Arsenic; C009941:potassium thiocyanate; C022842:chloric acid