Effects of CO2 insufflation on bacterial growth in rats with Escherichia coli-induced experimental peritonitis.
The effects of i.p. CO2 insufflation on bacterial proliferation in a setting of Escherichia coli-induced experimental peritonitis was studied in a rat model. Six male Wistar rats were given 0.25 ml of i.p. saline and formed the sham operation group. Twenty-four rats were divided into three groups, and all had i.p. E. coli injections. Microorganism counts were taken after 8 h in all groups. Group 1 was used as the control group. Group 2 (laparoscopy) was insufflated with CO2, and group 3 (laparotomy) had a midline laparotomy. Microorganism counts were repeated 8 h after the procedures (16 h after i.p. E. coli inoculation). Postoperative microorganism counts were significantly higher in the CO2 insufflation group (p < 0.05) compared with the control and laparotomy groups and showed an increase, whereas they decreased in the other two groups.
Sare
M
M
Department of General Surgery, Faculty of Medicine, Inönü University, Malatya, Turkey.
Yesilada
O
O
Gürel
M
M
Balkaya
M
M
Yologlu
S
S
Fiskin
K
K
D000818:Animals; D002245:Carbon Dioxide / Q000008:administration & dosage / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D004927:Escherichia coli Infections / Q000382:microbiology*; D010535:Laparoscopy; D007813:Laparotomy; D008297:Male; D010538:Peritonitis / Q000382:microbiology*; D051381:Rats; D017208:Rats, Wistar
D002245:Carbon Dioxide