5-HT2B receptor mRNA in guinea pig superior cervical ganglion.
Primers for 5-HT2A, 5-HT2B and 5-HT2C receptor mRNAs were used in reverse transcriptase-linked polymerase chain reactions (RT-PCR) to determine the presence of these transcripts in the guinea pig superior cervical ganglion. This was done to help identify an as yet unknown 5-HT2-like receptor which, in addition to 5-HT2A receptors, mediates a slow depolarization of this preparation. PCR products corresponding to 5-HT2A and 5-HT2B, but not 5-HT2C, receptor mRNA could readily be detected. Subsequent sequence analysis of these products confirmed that the 5-HT2A band corresponded to part of the guinea pig 5-HT2A receptor and the 5-HT2B band probably represents a portion of the guinea pig 5-HT2B receptor. The latter sequence shares greater homology with an equivalent region of the human than the rat 5-HT2B receptor.
Newberry
N R
NR
Oxford University, SmithKline Beecham Centre for Applied Neuropsychobiology, University Department of Clinical Pharmacology, Radcliffe Infirmary, UK.
Watkins
C J
CJ
Volenec
A
A
Flanigan
T P
TP
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000235:genetics; D017783:Superior Cervical Ganglion / Q000378:metabolism*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011985:Receptors, Serotonin