Covalent attachment of hybridizable oligonucleotides to glass supports.
A simple, rapid, and efficient method for the covalent binding of oligonucleotides to solid glass supports was developed. Glass slides were derivatized with aminophenyl or aminopropyl silanes and 5'-succinylated target oligonucleotides were attached by carbodiimide-mediated coupling. Approximately 40 to 50% of the applied target oligonucleotides covalently bound to the derivatized glass. Hybridizations with radioactively labeled oligonucleotide probes showed that up to 90% of the attached oligonucleotides were available for hybridization. This system can conveniently be applied for studies on hybridization and detection of nucleic acids.
Joos
B
B
Department of Medicine, University Hospital, Zurich, Switzerland. infjob@usz.unizh.ch
Kuster
H
H
Cone
R
R
D001483:Base Sequence; D001665:Binding Sites; D005898:Glass; D015497:HIV-1 / Q000235:genetics; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D012821:Silanes
D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D012821:Silanes