Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli.
We identified proteins whose amounts were altered in a temperature-sensitive dnaA46 mutant of Escherichia coli. Proteins whose amounts were increased in the mutant were serine hydroxymethyltransferase, beta-ketoacyl [acyl carrier protein] synthase II, long-chain fatty acid transport protein, and UDP-glucose 4-epimerase, while the decreased ones were flagellin and D-ribose-binding protein. Transformation of the mutant with a plasmid containing the wild type dnaA gene complemented the phenotype. As pulse-labeling experiments revealed that the rates of synthesis of the proteins were altered in the mutant, DnaA protein may be involved in expression of these proteins.
Ohba
A
A
Faculty of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan.
Mizushima
T
T
Katayama
T
T
Sekimizu
K
K
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000096:biosynthesis; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D050612:Fatty Acid Transport Proteins; D005408:Flagellin / Q000096:biosynthesis; D005816:Genetic Complementation Test; D012696:Glycine Hydroxymethyltransferase / Q000096:biosynthesis; D008969:Molecular Sequence Data; D016296:Mutagenesis; D033902:Periplasmic Binding Proteins; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D012266:Ribose / Q000378:metabolism; D013696:Temperature; D014169:Transformation, Bacterial; D014534:UDPglucose 4-Epimerase / Q000096:biosynthesis
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D029968:Escherichia coli Proteins; D050612:Fatty Acid Transport Proteins; D033902:Periplasmic Binding Proteins; C028003:RbsB protein, E coli; D011994:Recombinant Proteins; C065691:fadL protein, E coli; D005408:Flagellin; D012266:Ribose; D012696:Glycine Hydroxymethyltransferase; D014534:UDPglucose 4-Epimerase