[Growth suppression of squamous cell carcinoma cell lines by PKCs--possible application to gene therapy].
Protein kinase C is a serine/threonine kinase protein, which consists of 12 isoforms. Among these isoforms, PKC eta is known to play an important role in epithelial differentiation. The present study was conducted to examine the possibility that the introduction of these genes causes growth suppression of squamous cell carcinoma. It was found that adenovirus vectors containing cDNA of PKC eta suppresses the cell growth of human oral mucosal keratinocytes and activates transglutaminase 1, a key enzyme of squamous cell differentiation. In an oral squamous cell carcinoma cell line, overexpression of this isoform did not suppress the growth, but TPA treatment resulted in suppression of cell proliferation. A dominant negative form of PKC eta did not suppress the growth of carcinoma cell lines even with TPA-treatment. Human fibroblasts showed no response to TPA-treatment. The same result was shown with PKC delta. The results of this study suggested the possibility of using PKC isoforms for gene therapy.
Fujii
M
M
Second Department of Oral Surgery, Faculty of Dentistry, Tokyo Medical and Dental University.
D000256:Adenoviridae / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002455:Cell Division / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D009062:Mouth Neoplasms / Q000473:pathology*; D011493:Protein Kinase C / Q000235:genetics*; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; C081975:protein kinase C eta; D011493:Protein Kinase C