Ouabain enhances the lipopolysaccharide-induced nitric oxide production by rat peritoneal macrophages.
Incubation of rat peritoneal macrophages with the bacterial product lipopolysaccharide (LPS) caused significant increases in the production of nitrite, a stable endproduct of nitric oxide (NO). Addition of the digitalis-derived glycoside ouabain, which alone is without effect, significantly enhanced the stimulating effects of LPS. The Na+ ionophore monensin was without any effect, while the Ca2+ ionophore A23187, like ouabain, enhanced the effect of LPS. Thus ouabain may enhance the LPS-stimulated NO production in rat peritoneal macrophages via still unknown mechanisms in which Ca2+ ions are most likely involved.
Sowa
G
G
Department of Molecular Neuropharmacology, Polish Academy of Sciences, Krakow, Poland.
Przewłocki
R
R
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D008985:Monensin / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis*; D009573:Nitrites / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013268:Stimulation, Chemical
D004791:Enzyme Inhibitors; D007476:Ionophores; D008070:Lipopolysaccharides; D009573:Nitrites; D009569:Nitric Oxide; D000001:Calcimycin; D010042:Ouabain; D008985:Monensin; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium