title: Neutral glycosphingolipids and gangliosides from spleen T lymphoblasts of genetically different inbred mouse strains.
abstract: The gangliosides G(M1b), GalNAc-G(M1b) and G(D1alpha) are typical compounds of concanavalin A stimulated splenic T lymphoblasts of CBA/J inbred mice. Their structural characterization has been described in previous studies. The intention of this work was the comparative TLC immunostaining analysis of the glycosphingolipid composition of lectin stimulated splenic T lymphoblasts obtained from six genetically different inbred mouse strains. The strains examined were AKR, BALB/c, C57BL/6, CBA/J, DBA/2 and WHT/Ht, which are commonly used for biochemical and immunological studies. The neutral glycosphingolipid GgOse4Cer, the precursor for G(M1b)-type gangliosides, was expressed by all six strains investigated. AKR, C57BL/6 and DBA/2 showed high and BALB/c, CBA/J and WHT/Ht diminished expression in T lymphoblasts, based on single cell calculation. The gangliosides G(M1b) and GalNAc-G(M1b), elongation products of GgOse4Cer, displayed strain-specific differences in their intensities, which were found to correlate with the intensities of GgOse4Cer expression of the same strains. Concerning sialic acid substitution of gangliosides, G(M1b) and GalNAc-G(M1b) predominantly carry N-acetylneuraminic acid, whereas choleragenoid receptors G(M1a) and Gal-GalNAc-G(M1b), which are also expressed by all six strains, are characterized by dominance of N-glycolylneuraminic acid. Two highly polar gangliosides, designated with X and Y, which have not been previously recognized in murine lymphoid tissue, were detected by positive anti-GalNAc-G(M1b) antibody and choleragenoid binding, respectively. Both gangliosides were restricted to AKR, DBA/2 and C57BL/6 mice. The other three strains BALB/c, CBA/J and WHT/Ht are lacking these structures. In summary, the G(M1b)-type pathway is quite active in all six strains analysed in this study. Strain-specific genetic variations in T lymphoblast gangliosides were observed with the occurrence of gangliosides X and Y. This study and data from other groups strongly indicate for G(M1b)-type gangliosides a functional association with T cell activation and leukocyte mediated reactions.
authors.affiliation: Institute of Cell Culture Technology, University of Bielefeld, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Müthing
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002772:Cholera Toxin / Q000276:immunology / Q000378:metabolism; D002855:Chromatography, Thin Layer / Q000379:methods; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D005677:G(M1) Ganglioside / Q000737:chemistry / Q000276:immunology; D005732:Gangliosides / Q000737:chemistry* / Q000276:immunology; D006028:Glycosphingolipids / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D013194:Staining and Labeling / Q000379:methods; D013601:T-Lymphocytes / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology
chemical_list: D005732:Gangliosides; D006028:Glycosphingolipids; D003208:Concanavalin A; D005677:G(M1) Ganglioside; D002772:Cholera Toxin