The site for GTP hydrolysis on the archaeal elongation factor 2 is unmasked by aliphatic alcohols.
An appropriate mixture of ethylene glycol and BaCl2 enhanced the otherwise very low intrinsic GTPase activity of the elongation factor 2 isolated from the archaeon Sulfolobus solfataricus (SsEF-2). The enzymatic activity became up to 300-fold higher than that of the SsEF-2 GTPase measured in the absence of any stimulator, but remained 20-fold lower than that stimulated by ribosome. The stimulatory effect of ethylene glycol/Ba2+ was attributed to the increased affinity for GTP, probably related to a conformational change occurring in a hydrophobic region near the catalytic site.
Raimo
G
G
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy.
Masullo
M
M
Scarano
G
G
Bocchini
V
V
D000246:Adenosine Diphosphate Ribose; D000438:Alcohols / Q000494:pharmacology*; D017609:Barium Compounds / Q000494:pharmacology; D001665:Binding Sites; D002413:Cations, Divalent / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000494:pharmacology; D020963:GTP Phosphohydrolase-Linked Elongation Factors / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000378:metabolism* / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000187:drug effects / Q000378:metabolism*; D016944:Sulfolobus / Q000201:enzymology*; D013696:Temperature
D000438:Alcohols; D017609:Barium Compounds; D002413:Cations, Divalent; D002712:Chlorides; D005026:Ethylene Glycols; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; C024986:barium chloride; D006153:Guanosine Diphosphate; D000246:Adenosine Diphosphate Ribose; D006165:Guanylyl Imidodiphosphate; D006160:Guanosine Triphosphate; D020963:GTP Phosphohydrolase-Linked Elongation Factors; D019204:GTP-Binding Proteins; D019855:Ethylene Glycol