title: Evaluation of seven commercially available ELISA kits for serodiagnosis of acute toxoplasmosis.
abstract: Seven Toxoplasma gondii IgM antibody detection kits commercially available in the Indian markets were evaluated for their sensitivity, specificity, predictive values and cost-effectiveness on 140 serum samples (80 sera were positive and 60 negative). Modified direct agglutination test was used as standard. The principals of seven anti-Toxoplasma gondii IgM antibody detection kits were Organon-Teknika (Holland), Eurogenetics (Belgium), Labo Biomedical (Netherlands), Gamma-sa (Belgium), Viro-Immun Labor Diagnostika (Germany), Diamedix (USA) and Melotech (Spain). Of the seven kits, the first 4 were based on mu-capture principle. Organon-Teknika IgM ELISA kit was found to be most accurate, cost-effective and presentable, while Melotech and Viro-immun IgM kits were found unacceptable for clinical diagnosis of toxoplasmosis.
authors.affiliation: Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi.
authors.forename: S
authors.initials: S
authors.lastname: Singh
authors.forename: N
authors.initials: N
authors.lastname: Singh
authors.forename: S N
authors.initials: SN
authors.lastname: Dwivedi
mesh_terms: D000208:Acute Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012698:Serologic Tests / Q000379:methods*; D014123:Toxoplasmosis / Q000175:diagnosis*
chemical_list: D011933:Reagent Kits, Diagnostic