title: Signal sequence trap. Expression cloning method for secreted proteins and type 1 membrane proteins.
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tashiro
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Honjo
mesh_terms: D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers; D018076:DNA, Complementary; D016208:Databases, Factual; D015723:Gene Library; D017930:Genes, Reporter; D006801:Humans; D007202:Indicators and Reagents; D008565:Membrane Proteins / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence / Q000379:methods; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D021382:Protein Sorting Signals / Q000096:biosynthesis*; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis*; D016324:Sequence Tagged Sites; D014162:Transfection / Q000379:methods
chemical_list: D000906:Antibodies; D017931:DNA Primers; D018076:DNA, Complementary; D007202:Indicators and Reagents; D008565:Membrane Proteins; D021382:Protein Sorting Signals; D015375:Receptors, Interleukin-2; D011993:Recombinant Fusion Proteins