HLA class II DNA typing in Buerger's disease.
To analyze the genetic factors involved in the pathogenesis of Buerger's disease, the polymorphisms of HLA class II genes (HLA-DP, -DQ and -DRB) were investigated in 36 patients using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. HLA class I (B54 and B52) and HLA class II (DQ alleles) genes were also typed by polymerase chain reaction-sequence-specific oligonucleotide probe (PCR SSOP) method to confirm alleles. PCR-RFLP typing revealed that the frequencies of HLA class II genes, DRB1*0405, DQB1*0401, DQA1*03 and DPB1*0501, were significantly increased in the patients with Buerger's disease compared with the healthy controls. Further, DNA typing confirmed that HLA-B54 was associated with Buerger's disease. These results suggested that the haplotype of HLA-B54-DRB1*0405-DQB1*0401- DQA1*03-DPB1*0501 was associated with Buerger's disease in Japanese population.
Aerbajinai
W
W
Department of Molecular Genetics, Tokyo Medical and Dental University, Japan.
Tsuchiya
T
T
Kimura
A
A
Yasukochi
Y
Y
Numano
F
F
D000328:Adult; D000483:Alleles; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics; D059950:HLA-B52 Antigen; D006683:HLA-DQ Antigens / Q000235:genetics; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D007564:Japan; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013919:Thromboangiitis Obliterans / Q000235:genetics*
D005819:Genetic Markers; D015235:HLA-B Antigens; D059950:HLA-B52 Antigen; D006683:HLA-DQ Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II