Reactivity of Trypanosoma cruzi strains with peanut agglutinin (PNA) correlates with number of in vitro infected host cells.
Reactivities of 4 lectins with intact trypomastigote forms derived from 8 different Trypanosoma cruzi strains were compared with their capacity to infect in vitro cultured LLC-MK(2)cells. A sensitive and reproducible titration method for lectin binding sites (ELLA: Enzyme Linked Lectin Assay) was employed, in which reactivities were scored through optical densities in an ELISA reader. Tissue culture trypomastigotes from the strains Y, CL, SC4, SC24, SC25, SC28, SC32 and SC33 were investigated for expression of different cell surface carbohydrate residues using Concanavalin A (ConA), Peanut agglutinin (PNA), Soybean agglutinin (SBA) and Wheat germ agglutinin (WGA) conjugated to peroxidase. The reactivity of the strains to PNA lectin was SC28 > SC32 > SC33 > SC25> SC24 > Y> CL> SC4. The optical density values obtained were highly correlated (r2=0.986, p< 10(-4)) with the number of parasitized LLC-MK(2) cells 24 hours after infection by trypomastigotes from each corresponding strain. We concluded that galactose and N-acetyl-D-galactosamine residues that are present on the surface of trypomastigotes are important in host-cell recognition.
Piazza
R M
RM
Laboratorio de Imunoparasitologia, Instituto Butantan, Sao Paulo, Brazil.
Borges
M M
MM
Kloetzel
J K
JK
Stolf
A M
AM
D000818:Animals; D010367:Arachis / Q000737:chemistry*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D037102:Lectins / Q000378:metabolism*; D009005:Monosaccharides / Q000032:analysis / Q000494:pharmacology; D019887:Peanut Agglutinin; D037121:Plant Lectins; D012680:Sensitivity and Specificity; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000378:metabolism* / Q000472:pathogenicity*
D037102:Lectins; D009005:Monosaccharides; D019887:Peanut Agglutinin; D037121:Plant Lectins