Use of bovine myeloperoxidase as an indicator of mastitis in dairy cattle.
Bovine myeloperoxidase (MPO) is a heme protein consisting of both large and small polypeptide subunits. In mammals the role of MPO in defending against microbes is well documented. To evaluate the potential of using MPO in the diagnosis of udder infections in dairy cattle we developed a specific enzyme immunoassay for bovine MPO in milk. Antibodies against bovine MPO were produced using the purified enzyme. The ELISA utilizes two specific antibodies: one that is anti-MPO monoclonal and one that is anti-MPO polyclonal. For a total of 141 milk samples the correlation coefficient between the somatic cell count and MPO concentrations determined using the ELISA was 0.91. The ELISA showed good precision and accuracy in measuring MPO in milk, with a total variation of ca. 10%. The recoveries of known amounts of MPO from milk were satisfactory. Thus the stability of the enzyme in milk was judged to be good. Microorganisms were isolated in ca. 85% of the milk samples with elevated concentrations of MPO. Microorganisms were not isolated from more than 90% of non mastitic milk samples with low somatic cell counts where MPO was not detectable using ELISA. The results clearly show that the quantitative analysis of the amount of MPO in mastitic milk can be used to detect intramammary infections in dairy cattle.
Cooray
R
R
Mastitis Laboratory, National Veterinary Institute, Uppsala, Sweden.
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002417:Cattle; D004796:Clinical Enzyme Tests / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000382:microbiology; D008892:Milk / Q000201:enzymology* / Q000382:microbiology; D009195:Peroxidase / Q000032:analysis*; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D009195:Peroxidase