Development and characterization of SV40 immortalized rat submandibular acinar cell lines.
Rat submandibular salivary gland acinar cells were transfected by CaPO4 precipitation using a plasmid containing a replication-defective simian virus (SV40) genome. Out of 27 clonal cell lines, two were shown to have moderate to high levels of cytodifferentiation and salivary gland acinar cell function. Functional studies with the two cell lines indicated that the beta-adrenergic agonist, isoproterenol, vasoactive intestinal peptide, and prostaglandin E1 were effective activators of intracellular cyclic AMP production. Epinephrine, norepinephrine, phenylephrine, acetylcholine, and P2U-purinoceptor agonists were effective in increasing inositol phosphate production and intracellular free calcium levels, whereas substance P was without effect. Utilizing indirect immunofluorescence analysis, both cell lines were shown to express glutamine/glutamic acid-rich proteins, a submandibular acinar cell specific secretory protein family. Electron microscopic evaluation documented the maintenance of tripartite junctional complexes, cellular polarization, and the presence of moderate amounts of secretory granules and rough endoplasmic reticulum. The two cell lines had doubling times of 25 h.
Quissell
D O
DO
Department of Basic Sciences and Oral Research, School of Dentistry, University of Colorado Health Sciences Center, Denver 80262, USA.
Barzen
K A
KA
Gruenert
D C
DC
Redman
R S
RS
Camden
J M
JM
Turner
J T
JT
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed; D000242:Cyclic AMP / Q000096:biosynthesis; D019084:Fluorescent Antibody Technique, Indirect; D007295:Inositol Phosphates / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D010656:Phenylephrine; D010957:Plasmids; D051381:Rats; D017207:Rats, Sprague-Dawley; D013539:Simian virus 40 / Q000235:genetics*; D013363:Submandibular Gland / Q000166:cytology* / Q000187:drug effects; D014162:Transfection
D007295:Inositol Phosphates; D010656:Phenylephrine; D000242:Cyclic AMP; D002118:Calcium