title: Regulation of colonic H-K-ATPase in large intestine and kidney by dietary Na depletion and dietary K depletion.
abstract: Active K absorption in the rat distal colon is energized by an apical membrane H-K-ATPase, whereas K absorption in the distal collecting duct is generally believed to be modulated by a related renal H-K-ATPase. Experiments were performed to establish the mechanism(s) by which dietary Na depletion (with resulting elevated aldosterone levels) and K depletion stimulate K absorption. A colonic H-K-ATPase-specific cDNA probe and a polyclonal antibody were utilized to measure mRNA (Northern blot analyses) and protein (Western blot and immunofluorescence studies) abundance in the distal and proximal colon and renal collecting ducts and cortex of dietary Na- and K-depleted rats. Dietary Na depletion, but not K depletion, upregulated H-K-ATPase-specific mRNA and protein expression in the distal and proximal colon; Na depletion also stimulated H-K-ATPase activity in the distal colon. In contrast to the distal colon, H-K-ATPase-specific protein level in the outer medulla was enhanced by dietary K depletion, but not by Na depletion. This study establishes that 1) dietary Na depletion stimulates colonic H-K-ATPase activity most likely by a transcriptional process and 2) the regulation of colonic H-K-ATPase expression by dietary Na depletion and dietary K depletion is not identical in the large intestine and differs in the kidney from the colon, suggesting the presence of two (or more) H-K-ATPase isoforms in the rat colon.
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
authors.forename: P
authors.initials: P
authors.lastname: Sangan
authors.forename: V M
authors.initials: VM
authors.lastname: Rajendran
authors.forename: A S
authors.initials: AS
authors.lastname: Mann
authors.forename: M
authors.initials: M
authors.lastname: Kashgarian
authors.forename: H J
authors.initials: HJ
authors.lastname: Binder
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D003106:Colon / Q000201:enzymology*; D004032:Diet; D017506:H(+)-K(+)-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D011191:Potassium Deficiency / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000172:deficiency*; D013270:Stomach / Q000201:enzymology
chemical_list: D012333:RNA, Messenger; D012964:Sodium; D017506:H(+)-K(+)-Exchanging ATPase