In vivo retrovirus-mediated gene transfer into multiple hematopoietic lineages in rabbits without preconditioning.
Hematopoietic progenitor cells are attractive targets for gene therapy of inherited and acquired disorders. We have developed a novel procedure for mediating gene transfer into hematopoietic cells using an in vivo approach. The procedure involves injection of irradiated retroviral producer cells into the femoral bone marrow cavity in rabbits without preconditioning. The emergence of vector-marked cells in multiple peripheral blood hematopoietic lineages was detected 1 week post-injection and persisted until the animals were sacrificed up to 20 months later. Vector-marked cells were also detected in different hematopoietic tissues including bone marrow, spleen, thymus, and lymph node. Expression of retrovirus-specific messages by reverse transcription polymerase chain reaction was detected at multiple time points up to 20 months. Retrovirally encoded protein was detected by enzyme-linked immunosorbent assay in supernatant from cultures of granulocytes isolated 14 months after the procedure. This work demonstrates the feasibility of effecting gene transfer into hematopoietic progenitor cells in vivo.
Nelson
D M
DM
Gene Transfer Technology Section, National Center for Human Genome Research, National Institute of Health, Bethesda, MD 20892, USA.
Metzger
M E
ME
Donahue
R E
RE
Morgan
R A
RA
D000818:Animals; D001853:Bone Marrow; D019070:Cell Lineage; D005164:Factor IX / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction; D011817:Rabbits; D015203:Reproducibility of Results; D012190:Retroviridae
D005164:Factor IX