title: Conservation of the 15-kilodalton lipoprotein among Treponema pallidum subspecies and strains and other pathogenic treponemes: genetic and antigenic analyses.
abstract: The 15-kDa lipoprotein of Treponema pallidum is a major immunogen during natural syphilis infection in humans and experimental infection in other hosts. The humoral and cellular immune responses to this molecule appear late in infection as resistance to reinfection is developing. One therefore might hypothesize that this antigen is important for protective immunity. This possibility is explored by using both genetic and antigenic approaches. Limited or no cross-protection has been demonstrated between the T. pallidum subspecies and strains or between Treponema species. We therefore hypothesized that if the 15-kDa antigen was of major importance in protective immunity, it might be a likely site of antigenic diversity. To explore this possibility, the sequences of the open reading frames of the 15-kDa gene have been determined for Treponema pallidum subsp. pallidum (Nichols and Bal-3 strains), T. pallidum subsp. pertenue (Gauthier strain), T. pallidum subsp. endemicum (Bosnia strain), Treponema paraluiscuniculi (Cuniculi A, H, and K strains), and a little-characterized simian isolate of Treponema sp. (Fribourg-Blanc strain). No significant differences in DNA sequences of the genes for the coding region of the 15-kDa antigen were found among the different species and subspecies studied. In addition, all organisms showed expression of the 15-kDa antigen as determined by monoclonal antibody staining. The role of the 15-kDa antigen in protection against homologous infection with T. pallidum subsp. pallidum Nichols was examined in rabbits immunized with a purified recombinant 15-kDa fusion protein. No alteration in chancre development was observed in immunized, compared to unimmunized, rabbits, and the antisera induced by the immunization failed to enhance phagocytosis of T. pallidum subsp. pallidum by macrophages in vitro. These results do not support a major role for this antigen in protection against syphilis infection.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195, USA.
authors.forename: A
authors.initials: A
authors.lastname: Centurion-Lara
authors.forename: T
authors.initials: T
authors.lastname: Arroll
authors.forename: R
authors.initials: R
authors.lastname: Castillo
authors.forename: J M
authors.initials: JM
authors.lastname: Shaffer
authors.forename: C
authors.initials: C
authors.lastname: Castro
authors.forename: W C
authors.initials: WC
authors.lastname: Van Voorhis
authors.forename: S A
authors.initials: SA
authors.lastname: Lukehart
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D017124:Conserved Sequence; D019143:Evolution, Molecular; D006801:Humans; D008074:Lipoproteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D011817:Rabbits; D016415:Sequence Alignment; D014210:Treponema pallidum / Q000235:genetics* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008074:Lipoproteins