title: Expression of a recombinant human growth hormone binding protein in baculovirus/insect cell system.
abstract: An eucaryotic recombinant human growth hormone binding protein (rGHBP) was expressed in baculovirus-infected insect cells and purified by affinity chromatography from culture supernatant. This mannose-rich 34-kDa protein specifically bound human growth hormone (hGH) with the same affinity (kDa = 0.42 x 10(-9) M) than the 51.5 kDa GHBP we purified and characterised from human plasma (kDa = 1.1 x 10(-9) M). A high molecular form of the rGHBP was detected by silver-stained SDS-PAGE, Western blot (mAb 263), affinity cross-linking and Western ligand blot with 125I-hGH. Reduction experiments with beta-mercaptoethanol suggested that this form involved a disulfide bound between two rGHBPs.
authors.affiliation: Centre de Recherche sur la Croissance, Centre Hospitalier Universitaire de Purpan, Toulousel Pharmacia France.
authors.forename: S
authors.initials: S
authors.lastname: Cholin
authors.forename: E
authors.initials: E
authors.lastname: Bieth
authors.forename: C
authors.initials: C
authors.lastname: Molinas
authors.forename: C
authors.initials: C
authors.lastname: Cahoreau
authors.forename: C
authors.initials: C
authors.lastname: Perals
authors.forename: M
authors.initials: M
authors.lastname: Cerruti
authors.forename: G
authors.initials: G
authors.lastname: Devauchelle
authors.forename: P
authors.initials: P
authors.lastname: Rochiccioli
authors.forename: M
authors.initials: M
authors.lastname: Tauber
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors; D006031:Glycosylation; D019382:Human Growth Hormone; D006801:Humans; D007457:Iodine Radioisotopes; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018411:Spodoptera / Q000166:cytology
chemical_list: D002352:Carrier Proteins; D007457:Iodine Radioisotopes; D011993:Recombinant Fusion Proteins; D019382:Human Growth Hormone; C051815:somatotropin-binding protein