Ultrastructural changes in chondriome of human lymphocytes after irradiation with He-Ne laser: appearance of giant mitochondria.
The structure of mitochondria was studied after the irradiation of human lymphocytes with an He-Ne laser (wavelength, 632.8 nm; dose, 56 J m-2). Ultrathin sections of the lymphocytes were studied by electron microscopy 1 h after the irradiation. The irradiation resulted in a 20% increase (p = 0.95) in the number of mitochondrial profiles on the cell section without an increase in their total area. Three-dimensional reconstruction of mitochondria from ultrathin sections trough the whole lymphocyte showed that the number of mitochondria was reduced to 9-12 in the irradiated cells compared with 40-45 in the control cells. In the irradiated lymphocytes, 2-4 giant branching mitochondria were also observed among small discrete mitochondria.
Manteifel
V
V
Laser Technology Research Center of the Russian Academy of Sciences, Troitsk, Moscow Region, Russian Federation.
Bakeeva
L
L
Karu
T
T
D006371:Helium; D006801:Humans; D007834:Lasers; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000528:radiation effects / Q000648:ultrastructure; D009356:Neon
D006371:Helium; D009356:Neon