title: Extracellular pH affects regulation of the pcbAB gene in Penicillium chrysogenum.
abstract: The effect of extracellular pH and dissolved oxygen on regulation of the pcbAB gene in P. chrysogenum was examined, using Northern analysis and a reporter gene fusion. It was found that ambient pH markedly affected levels of pcbAB mRNA whereas maintenance of dissolved oxygen concentration above 10% had no detectable effect. The presence of a DNA-binding protein, which binds upstream of the pcbAB translational start codon, was also related to ambient pH. In all fermentations, pcbAB mRNA was most abundant at around the late exponential/early stationary phase of a culture.
authors.affiliation: University of Westminster, School of Biological and Health Sciences, London, UK.
authors.forename: Y W
authors.initials: YW
authors.lastname: Chu
authors.forename: D
authors.initials: D
authors.lastname: Renno
authors.forename: G
authors.initials: G
authors.lastname: Saunders
mesh_terms: D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000494:pharmacology; D010408:Penicillium chrysogenum / Q000235:genetics*; D010453:Peptide Synthases / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D010453:Peptide Synthases; C095368:alpha-aminoadipyl-cysteinyl-valine synthetase; D010100:Oxygen