Detection of oncostatin M in synovial fluid from patients with rheumatoid arthritis.
OBJECTIVE
To measure oncostatin M (OSM) in synovial fluid from patients with rheumatoid arthritis (RA) and osteoarthritis (OA).


METHODS
20 samples of synovial fluid from patients with RA and 10 samples from patients with OA were examined using an OSM specific sandwich ELISA.


RESULTS
OSM was detected at concentrations ranging from 2.36 to 901.82 pg/ml in 18 (90%) of 20 samples of synovial fluid from RA patients. There was no detectable OSM in synovial fluid from OA patients. In the RA patients, the OSM concentration in synovial fluid correlated significantly with the synovial fluid white blood cell count (r = 0.67, p < 0.01), but not with other laboratory parameters of disease activity.


CONCLUSIONS
These findings suggest that OSM may contribute to joint inflammation in RA.
Hui
W
W
Department of Rheumatology, Royal Perth Hospital, Australia.
Bell
M
M
Carroll
G
G
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000276:immunology; D016207:Cytokines / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006131:Growth Inhibitors / Q000032:analysis*; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D053683:Oncostatin M; D010003:Osteoarthritis / Q000276:immunology; D010455:Peptides / Q000032:analysis*; D013582:Synovial Fluid / Q000737:chemistry*
D016207:Cytokines; D006131:Growth Inhibitors; C508351:OSM protein, human; D010455:Peptides; D053683:Oncostatin M