Phenotypic and functional differences between human dendritic cells derived in vitro from hematopoietic progenitors and from monocytes/macrophages.
We compared dendritic cells (DC) derived from CD34+ hematopoietic progenitor cells with tumor necrosis factor alpha and granulocyte-macrophage colony-stimulating factor (GM-CSF) to DC derived from monocytes/macrophages with interleukin-4 (IL-4) and GM-CSF. Monocyte/macrophage-derived DC demonstrated higher levels of CD1a, lower levels of CD14, greater stimulatory activity in mixed lymphocyte reactions, and greater capacity to present soluble protein antigen than CD34+ cell-derived DC. Lymphocytes stimulated with antigen-pulsed, monocyte/macrophage-derived DC produced more IL-10 than those stimulated with antigen-pulsed, CD34+-derived DC. Whereas CD1a+ DC could be derived from CD34+ cells in serum-free- and human-sera-containing cultures, the derivation of CD1a+ DC from monocytes/macrophages required the presence of fetal calf serum. The spectrum of cytokine mRNA expression, the presentation of peptide antigen, and the sensitivity to human immunodeficiency virus-1 infection of CD34(+)- and monocyte/macrophage-derived DC were comparable. Although cells derived by both methods are potent antigen-presenting cells, there are differences between DC derived in vitro from hematopoietic progenitors and from monocytes/macrophages that may influence their in vivo activity.
Triozzi
P L
PL
The Ohio State University Arthur G. James Cancer Hospital and Research Institute, Columbus, USA.
Aldrich
W
W
D000595:Amino Acid Sequence; D017951:Antigen Presentation; D018952:Antigens, CD34 / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D015658:HIV Infections / Q000473:pathology; D015497:HIV-1; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000166:cytology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology*; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism
D018952:Antigens, CD34; D016207:Cytokines; D012333:RNA, Messenger