Comprehensive on-line LC/LC/MS of proteins.
This is a description of a comprehensive two-dimensional liquid chromatography (LC) system for the separation of protein mixtures. This system uses cation-exchange chromatography followed by reversed-phase chromatography (RPLC). The two LC systems are coupled by an eight-port valve equipped with two storage loops and under computer control. The RPLC effluent is sampled by both a UV detector and an electrospray mass spectrometer. In this way, complex mixtures of large biomolecules can be rapidly separated, desalted, and analyzed for molecular weight in less than 2 h. The system's utility is demonstrated with a mixture of standards and an Escherichia coli cell lysate.
Opiteck
G J
GJ
Department of Chemistry, University of North Carolina at Chapel Hill 27599-3290, USA.
Lewis
K C
KC
Jorgenson
J W
JW
Anderegg
R J
RJ
D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004926:Escherichia coli / Q000737:chemistry; D013058:Mass Spectrometry; D008970:Molecular Weight; D009862:Online Systems; D011506:Proteins / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D011506:Proteins