Structure of the capsular polysaccharide from Alteromonas nigrifaciens IAM 13010T containing 2-acetamido-2,6-dideoxy-L-talose and 3-deoxy-D-manno-octulosonic acid.
A capsular polysaccharide was obtained from Alteromonas nigrifaciens IAM 13010T by saline extraction. On the basis of 1H and 13C NMR spectroscopy, including one-dimensional (1D) NOE spectroscopy, 2D rotating-frame NOE spectroscopy (ROESY), and 1H-detected heteronuclear 1H,13C multiple-quantum coherence (HMQC), it was concluded that the polysaccharide contained inter alia an acidic sugar, 3-deoxy-D-manno-octulosonic acid (Kdo), and a rare amino sugar, 2-acetamido-2,6-dideoxy-L-talose (L-6dTalNAc, N-acetylpneumosamine), and has a pentasaccharide repeating unit of the following structure: [equation: see text]
Gorshkova
R P
RP
Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russian Federation.
Nazarenko
E L
EL
Zubkov
V A
VA
Shashkov
A S
AS
Knirel
Y A
YA
Paramonov
N A
NA
Meshkov
S V
SV
Ivanova
E P
EP
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006088:Gram-Negative Aerobic Bacteria / Q000737:chemistry / Q000254:growth & development / Q000276:immunology*; D006595:Hexosamines / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D013400:Sugar Acids / Q000032:analysis
D006595:Hexosamines; C105842:N-acetylpneumosamine; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate