Stimulator of proliferation of spleen colony-forming cells in acute sterile inflammation.
The presence and activity of a spleen colony-forming cell (CFU-S) proliferation stimulator was investigated in rat bone marrow after induction of sterile inflammation. Wistar rats were treated intraperitoneally with two 15 ml injections of 3.5% polyvinylpyrrolidone (PVP) at 18 h intervals, and the presence of CFU-S proliferation stimulator determined in bone marrow 6, 20 and 24 h after the first injection. The marrow of these mice was used to condition medium which was then fractionated using Amicon Diaflo ultrafiltration membranes. The 30-50 kDa fraction, taken from 20h post-PVP-bone marrow extract, was found to induce cycling of d8-CFU-S in normal mouse bone marrow. This activity was not related to the presence of interleukins-1, -2 or -6 like activities in the material tested. The results demonstrate the existence of CFU-S proliferation stimulator in the bone marrow of rats with sterile inflammation (i.e. in an in vivo tissue response to non-specific cell stimulation), similar to that originally described as a macrophage product in mouse bone marrow after treatment with a variety of cytotoxic agents.
Milenković
P
P
Institute for Medical Research, University of Beograd, Yugoslavia (Serbia).
Ivanović
Z
Z
Lukić
M L
ML
Kataranovski
M
M
Lord
B I
BI
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D017077:Culture Media, Conditioned; D006133:Growth Substances / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D051379:Mice; D011205:Povidone / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology*
D017077:Culture Media, Conditioned; D006133:Growth Substances; D011205:Povidone