Interleukin-13 responsiveness and interleukin-13 receptor expression in non-Hodgkin's lymphoma and reactive lymph node B cells. Modulation by CD40 activation.
Interleukin-13 (IL-13) responsiveness was examined in lymph node B cells from patients with non-Hodgkin's lymphoma (NHL) and patients with benign reactive immune disorders. Proliferation assays showed that NHL B cells from 8 of 21 patients responded to IL-13 in the absence of a co-activation signal. IL-13-unresponsive NHL B cells from 9 of the 13 remaining patients were induced to respond to IL-13 upon antibody-triggered CD40 activation, as did reactive B cells. Binding experiments with radiolabeled IL-13 revealed that the constitutive expression of IL-13 receptors (IL-13R) was associated with IL-13 responsiveness in the absence of CD40 activation. In IL-13-unresponsive cells, IL-13R expression was induced after CD40 activation. This effect was enhanced by IL-10, which was able to potentiate the IL-13 response of CD40-activated cells. Furthermore, IL-13 was found to increase the viability of cultured NHL cells, but not that of non-malignant cells. These results suggest that IL-13, which behaves as a potent co-factor for normal lymph node B cell activation, might provide growth and/or survival advantages to NHL B cells.
Billard
C
C
INSERM U. 448, Faculté de Médecine, Créteil, France.
Caput
D
D
Vita
N
N
Ferrara
P
P
Orrico
M
M
Gaulard
P
P
Boumsell
L
L
Bensussan
A
A
Farcet
J P
JP
D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism*; D002455:Cell Division; D006801:Humans; D018793:Interleukin-13 / Q000494:pharmacology*; D053661:Interleukin-13 Receptor alpha1 Subunit; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000378:metabolism*; D053660:Receptors, Interleukin-13
D019013:CD40 Antigens; C508181:IL13RA1 protein, human; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D018123:Receptors, Interleukin; D053660:Receptors, Interleukin-13