Identification and characterization of a retinoic acid-regulated human homologue of the unc-33-like phosphoprotein gene (hUlip) from neuroblastoma cells.
A cDNA, 7G1, was isolated from retinoic acid (RA) differentiated neuroblastoma cells whose expression was high in human fetal brain and spinal cord mRNA but undetectable in adult brain or non-neuronal tissues. Sequence analysis indicates that 7G1 is homologous to the Caenorhabditis elegans gene unc-33. A 5.5-kilobase pair full-length cDNA from a human fetal brain cDNA library contains an 1710-base pair open reading frame. Because the predicted 570 amino acid sequence of 7G1 shares 98% identity with the murine Ulip gene product, an unc-33-like-phosphoprotein, we refer to 7G1 as the human Ulip (hUlip). hUlip is also similar to the bacterial enzyme D-hydantoinase and the recently described vertebrate gene products CRMP62, TOAD-64, CRMP1, CRMP2, and mUNC. RA stimulates an increase in hUlip mRNA that is transcriptionally regulated. RA stimulates an increase in polypeptides of 58, 60, 65, and 70 kDa with the 58- and 65-kDa species being dephosphorylated forms of the 60- and 70-kDa species. This study presents a model in which to study the regulation and expression of the hUlip gene, a member of an emerging family of molecules that potentially mediates signals involved in axonal outgrowth.
Gaetano
C
C
Cell and Molecular Biology Section, Pediatric Oncology Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA.
Matsuo
T
T
Thiele
C J
CJ
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins; D002454:Cell Differentiation / Q000187:drug effects; D005333:Fetus; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D015801:Helminth Proteins / Q000737:chemistry; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins; D009414:Nerve Growth Factors / Q000737:chemistry; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000378:metabolism*; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014714:Vertebrates
D029742:Caenorhabditis elegans Proteins; C102926:DPYSL3 protein, human; D015801:Helminth Proteins; D009124:Muscle Proteins; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D010750:Phosphoproteins; D012333:RNA, Messenger; C078652:unc-33 protein, C elegans; D014212:Tretinoin