Interassay differences in growth hormone measurement in acromegaly.
We measured plasma growth hormone concentrations by three different two-site immunometric assays (Pharmacia hGH RIA, IDS Gamma-BCT and Delfia 22 kDa hGH) to determine whether there are GH isoforms secreted by acromegalic patients that are under-recognized by some assays. There was a fairly good agreement between assays with the IDS Gamma-BCT and Delfia 22 kDa assays giving lower results than the Pharmacia IRMA. GH was measured on stored plasma samples from 24 patients with proven acromegaly. There was a consistent difference between the three assays of approximately 20% of the mean value for each patient.
Barth
J H
JH
Department of Chemical Pathology and Immunology, Leeds General Infirmary, UK.
Smith
J H
JH
Clarkson
P
P
D000172:Acromegaly / Q000097:blood*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000032:analysis; D005260:Female; D019382:Human Growth Hormone / Q000097:blood* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D019382:Human Growth Hormone