title: Reversibility of monocytic differentiation of HL-60 cells by 1alpha,25 dihydroxyvitamin D3.
abstract: Although 1alpha,25(OH)2D3 induces HL-60 cells to differentiate into monocytes, whether or not these monocytes revert to native promyelocytes is not clear. To investigate this question, HL-60 cells were treated with 1alpha,25(OH)2D3, and divided into CD14-positive and -negative cells with a cell sorter. These two populations were cultured with or without 1alpha,25(OH)2D3. Sorted CD14-positive HL-60 cells, treated with 1alpha,25(OH)2D3 for 7 days, reverted to CD14-negative cells and promyelocyte-like cells if 1alpha,25(OH)2D3 was removed from the medium. We conclude that the 1alpha,25(OH)2D3-induced differentiation of HL-60 cells into monocytes is reversible, and continuous administration of 1alpha,25(OH)2D3 is required for the differentiation of HL-60 cells.
authors.affiliation: Department of Hematology, Showa University School of Medicine, Shinagawa-ku, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tomoyasu
authors.forename: K
authors.initials: K
authors.lastname: Fukuchi
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Ueno
authors.forename: Y
authors.initials: Y
authors.lastname: Hamano
authors.forename: J
authors.initials: J
authors.lastname: Hisatake
authors.forename: K
authors.initials: K
authors.lastname: Hino
authors.forename: K
authors.initials: K
authors.lastname: Gomi
authors.forename: N
authors.initials: N
authors.lastname: Tsuruoka
mesh_terms: D002117:Calcitriol / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D016130:Immunophenotyping; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D002117:Calcitriol