title: Role of platelet-activating-factor (PAF) on cellular responses after stimulation with leptospire lipopolysaccharide.
abstract: Leptospire lipopolysaccharide (LPS) stimulated the adherence of polymorphonuclear neutrophils (PMNs) to human umbilical vein endothelial cells (HUVEC). Enhanced PMN adherence in response to leptospire LPS can be mediated by platelet-activator-factor (PAF), because a PAF antagonist reduced adherence. Leptospire LPS also induced the adherence platelets or U937. The second experiment involved leptospire LPS elicited platelet aggregation in a PMN-platelet mixture, because leptospire LPS stimulated human PMN but not the human platelets. The platelet response was observed only in the mixture system and was inhibited by a PAF antagonist. PAF could be an important pathogenic factor in human leptospirosis.
authors.affiliation: Department of Preventive Dentistry, Health Sciences University of Hokkaido, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Isogai
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: H
authors.initials: H
authors.lastname: Isogai
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002449:Cell Aggregation; D004730:Endothelium, Vascular / Q000276:immunology; D006801:Humans; D007921:Leptospira interrogans / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D010972:Platelet Activating Factor / Q000276:immunology*; D010974:Platelet Aggregation; D011817:Rabbits; D012772:Shock, Septic / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D010972:Platelet Activating Factor