Human tau becomes phosphorylated and forms filamentous deposits when overexpressed in lamprey central neurons in situ.
Microinjection of plasmids encoding human tau (htau) protein into identified lamprey reticulospinal neurons (anterior bulbar cells, or ABCs) in situ induces chronic htau expression. htau protein is transported to both the axon and dendrites of expressing ABCs by mechanisms that require the C-terminal domain of htau protein but do not require directed htau mRNA transport. htau becomes phosphorylated at the PHF-1 (Ser-396/404) and TAU-1/AT8 (Ser-199/202) epitopes throughout ABCs with heavy htau accumulations; many such cells also exhibit degenerative changes, which include the development of extracellular htau deposits. Finally, expression of htau protein fused to green fluorescent protein induced the somatodendritic accumulation of filaments containing htau when examined by immunoelectron microscopy. These results suggest that chronic expression of htau in lamprey ABCs may be useful for studying cellular mechanisms underlying tau hyperphosphorylation and filament formation in vertebrate central neurons in situ.
Hall
G F
GF
Department of Biological Sciences, University of Massachusetts, Lowell, MA 01854, USA.
Yao
J
J
Lee
G
G
D000544:Alzheimer Disease / Q000209:etiology; D000818:Animals; D002490:Central Nervous System / Q000378:metabolism / Q000473:pathology*; D003712:Dendrites / Q000378:metabolism; D006801:Humans; D007798:Lampreys; D008845:Microinjections; D016874:Neurofibrillary Tangles; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D010766:Phosphorylation; D010957:Plasmids; D011994:Recombinant Proteins / Q000648:ultrastructure; D013045:Species Specificity; D016875:tau Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*
D011994:Recombinant Proteins; D016875:tau Proteins