Inhibitory effects of tenilsetam on the Maillard reaction.
It has been hypothesized that advanced Maillard reaction in vivo could explain some of the age- and diabetes-related changes. Furthermore, involvement of the Maillard reaction with Alzheimer's disease has also been suggested, as advanced glycation end products, such as pyrraline and pentosidine, were demonstrated to localize in lesions of the disease. Although aminoguanidine has been studied extensively and established as an inhibitor of the Maillard reaction, other candidates have not been investigated thoroughly. In the present study, we examined the inhibitory effect of tenilsetam [(+/-)-3-(2-thienyl)-2-piperazinone], an antidementia drug, on the Maillard reaction. Tenilsetam inhibited glucose- and fructose-induced polymerization of lysozyme in a concentration-dependent manner in vitro. Reduced enzymatic digestibility of collagen incubated with 100 mM glucose for 4 weeks was also restored to a control level by coincubation with 100 mM tenilsetam. To determine whether tenilsetam inhibits the Maillard reaction in vivo, streptozotocin-induced diabetic rats were treated with tenilsetam (50 mg/kg x day). Elevated levels of advanced glycation end-product-derived fluorescence and pyrraline in renal cortex and aorta of diabetic rats were suppressed by the administration of tenilsetam for 16 weeks. These inhibitory effects of this agent on advanced glycation in diabetic rats suggested its potential therapeutic role in controlling diabetic complications.
Shoda
H
H
Second Department of Internal Medicine, Kobe University School of Medicine, Chuo-ku, Japan.
Miyata
S
S
Liu
B F
BF
Yamada
H
H
Ohara
T
T
Suzuki
K
K
Oimomi
M
M
Kasuga
M
M
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006442:Glycated Hemoglobin / Q000378:metabolism; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D046911:Macromolecular Substances; D015416:Maillard Reaction; D008297:Male; D009113:Muramidase / Q000737:chemistry; D009646:Norleucine / Q000031:analogs & derivatives / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology*; D011619:Psychotropic Drugs / Q000494:pharmacology*; D011758:Pyrroles / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence; D013876:Thiophenes / Q000494:pharmacology*
D001786:Blood Glucose; D003094:Collagen; D003847:Deoxyglucose; D005632:Fructose; D005947:Glucose; D006442:Glycated Hemoglobin; D046911:Macromolecular Substances; D009113:Muramidase; D009646:Norleucine; D010879:Piperazines; D011619:Psychotropic Drugs; D011758:Pyrroles; D013876:Thiophenes; C060458:3-deoxyglucose; C059571:2-formyl-5-(hydroxymethyl)pyrrole-1-norleucine; C059511:tenilsetam