Long-lasting antibodies detected by a trans-sialidase inhibition assay of sera from parasite-free, serologically cured chagasic patients.
A test based on the inhibition by antibodies of the trans-sialidase was used to analyze infection by Trypanosoma cruzi, the agent of Chagas' disease. Sera collected during the longitudinal follow-up of benznidazole-treated acutely and congenitally infected patients became negative for T. cruzi as determined by tests presently used to assess cure; however, the sera remained positive for T. cruzi by the trans-sialidase inhibition assay (TIA) up to 14 years after treatment. Therefore, TIA is a highly sensitive marker for previous T. cruzi infection.
Leguizamón
M S
MS
Departamento de Microbiologia, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Russomando
G
G
Luquetti
A
A
Rassi
A
A
Almirón
M
M
González-Cappa
S M
SM
Frasch
A C
AC
Campetella
O
O
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001118:Argentina; D001938:Brazil; D014355:Chagas Disease / Q000151:congenital / Q000188:drug therapy / Q000276:immunology*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005500:Follow-Up Studies; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008137:Longitudinal Studies; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D009593:Nitroimidazoles / Q000627:therapeutic use; D010239:Paraguay; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic; D012016:Reference Values; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000276:immunology
D000913:Antibodies, Protozoan; D009593:Nitroimidazoles; D009439:Neuraminidase; C009999:benzonidazole