A pressure system for the microinjection of substances into the brain of awake monkeys.
We describe a method for placing pressure microinjections of drugs or anatomical tracers in physiologically defined sites in the brain of awake monkeys. This method provides a means to record neuronal activity from the tip of an injection cannula so that an injection can be made at a physiologically defined location. It uses pressure in a closed system to precisely control the amount of fluid injected and provides a visible means for monitoring injection volume. The injection cannula is easy to make with readily available components and can be used repeatedly for multiple recording sessions and injections.
Dias
E C
EC
Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208, USA.
Segraves
M A
MA
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000502:physiology*; D003243:Consciousness; D000882:Haplorhini; D008845:Microinjections / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D011312:Pressure; D015203:Reproducibility of Results