title: Effect of high- versus intermediate-purity blood coagulation factor concentrates on HIV-1 replication.
abstract: Controversy exists as to whether treatment of HIV-1-seropositive hemophiliacs with blood coagulation products of high purity might help prevent the decline of CD4-positive lymphocytes and thus delay progression toward AIDS. As viral load has recently been shown to be associated with disease progression in HIV-1 infection, we tested for a possible direct interference of high- or intermediate-purity blood coagulation products with replication of HIV-1. The data obtained revealed comparable replication of HIV-1 in the presence and absence of all blood coagulation products tested (assessed by PCR-based quantitation of proviral HIV-1 DNA in infected cells after a 10-day incubation period). These data suggest that coagulation factor concentrates per se will also have no direct effect on HIV-1 replication in vivo.
authors.affiliation: Institute of Immunology, University of Vienna, Austria.
authors.forename: D
authors.initials: D
authors.lastname: Schögl
authors.forename: K
authors.initials: K
authors.lastname: Zimmermann
authors.forename: P L
authors.initials: PL
authors.lastname: Turecek
authors.forename: H P
authors.initials: HP
authors.lastname: Schwarz
authors.forename: M M
authors.initials: MM
authors.lastname: Eibl
authors.forename: J W
authors.initials: JW
authors.lastname: Mannhalter
mesh_terms: D018791:CD4 Lymphocyte Count / Q000187:drug effects; D004279:DNA, Viral / Q000032:analysis; D018450:Disease Progression; D005169:Factor VIII / Q000302:isolation & purification* / Q000494:pharmacology*; D015658:HIV Infections / Q000150:complications*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006467:Hemophilia A / Q000150:complications* / Q000628:therapy; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D004279:DNA, Viral; D005169:Factor VIII