A new chelation method for determining ATPase activity in skeletal muscle.
Traditional methods for visualizing ATPase in sections use heavy metals that generate visible metal sulfide products. These methods use unpleasant and toxic reagents. We report a safer method using a novel ferric ion chelating agent to produce highly specific, low background, and permanent staining of muscle fiber enzymes.
Hawcroft
D M
DM
Department of Pharmaceutical Sciences, De Montfort University, Leicester, United Kingdom.
Ball
M T
MT
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D007502:Iron Chelating Agents / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000201:enzymology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D013053:Spectrophotometry / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
D007502:Iron Chelating Agents; C091526:ethyl N-methyl-4-hydroxy-5-oxo-3-pyrroline-3-carboxylate iron chelate; D000251:Adenosine Triphosphatases; D011761:Pyrrolidonecarboxylic Acid