Influence of transferrin glycans on receptor binding and iron-donation.
Human bi-bi-antennary transferrin (Tf) was partially deglycosylated by subsequently incubating with one or more of the following exoglycosidases: neuraminidase, beta-galactosidase or N-Acetyl-beta-D-glucosaminidase. Aglyco-Tf obtained from serum of a patient suffering from the Carbohydrate Deficient Glycoprotein syndrome was isolated. Receptor binding and the Tf and iron uptake capacities of the fully glycosylated-, partially deglycosylated- and aglyco-Tf were compared using the human hepatoma cell line PLC/PRF/5. No difference in binding capacity between the iso-Tf fractions could be demonstrated, however, the Tf and iron uptake capacity of aglyco-Tf was clearly reduced compared with the other Tf fractions.
Hoefkens
P
P
Department of Chemical Pathology, Erasmus University Rotterdam, The Netherlands.
Huijskes-Heins
M I
MI
de Jeu-Jaspars
C M
CM
van Noort
W L
WL
van Eijk
H G
HG
D000596:Amino Acids / Q000032:analysis; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D007501:Iron / Q000378:metabolism* / Q000493:pharmacokinetics; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism*; D014168:Transferrin / Q000737:chemistry* / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D043323:alpha-Mannosidase
D000596:Amino Acids; D002241:Carbohydrates; D011134:Polysaccharides; D011990:Receptors, Transferrin; D014168:Transferrin; D007501:Iron; D006026:Glycoside Hydrolases; D008361:Mannosidases; D043323:alpha-Mannosidase; D019158:N-Acetylneuraminic Acid; D008358:Mannose