title: A new chelation method for determining ATPase activity in skeletal muscle.
abstract: Traditional methods for visualizing ATPase in sections use heavy metals that generate visible metal sulfide products. These methods use unpleasant and toxic reagents. We report a safer method using a novel ferric ion chelating agent to produce highly specific, low background, and permanent staining of muscle fiber enzymes.
authors.affiliation: Department of Pharmaceutical Sciences, De Montfort University, Leicester, United Kingdom.
authors.forename: D M
authors.initials: DM
authors.lastname: Hawcroft
authors.forename: M T
authors.initials: MT
authors.lastname: Ball
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D007502:Iron Chelating Agents / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000201:enzymology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D013053:Spectrophotometry / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
chemical_list: D007502:Iron Chelating Agents; C091526:ethyl N-methyl-4-hydroxy-5-oxo-3-pyrroline-3-carboxylate iron chelate; D000251:Adenosine Triphosphatases; D011761:Pyrrolidonecarboxylic Acid