Influence of culture conditions on thermostable lipase production by a thermophilic alkalitolerant strain of Bacillus sp.
The effect of different culture conditions on thermostable lipase production by Bacillus sp. was studied in shake flasks. A maximum enzyme activity of 67-75 nkat/mL was observed in a medium consisting of 0.5% soybean flour and 0.1% stearyl glycerol esters or natural fats. A lipase activity of about 117 nkat/mL was established when the cultivation was carried out in a laboratory fermentor at 20% minimal dissolved oxygen level, the enzyme production being increased 1.5 fold compared to that in a flask culture.
Kambourova
M
M
Institute of Microbiology, Sofia, Bulgaria.
Emanuilova
E
E
Dimitrov
P
P
D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D001431:Bacteriological Techniques; D019149:Bioreactors; D003470:Culture Media; D004952:Esters / Q000378:metabolism; D005223:Fats / Q000378:metabolism; D008049:Lipase / Q000096:biosynthesis*; D013025:Glycine max / Q000378:metabolism
D003470:Culture Media; D004952:Esters; D005223:Fats; D008049:Lipase