Influence of debris and aggregates on image cytometry DNA measurement of archival tumors.
OBJECTIVE
To investigate, for seven samples, the influence of unwanted elements (e.g., remains of erythrocyte cell membranes, sliced nuclei, damaged nuclei and aggregates) on image cytometry DNA ploidy measurements.


METHODS
Two normal reference tissues (brain and breast), one breast cancer and four brain tumors were studied. For each sample, the influence of the different classes of debris on DNA ploidy histograms and indices was evaluated.


RESULTS
The influence differs regarding each class of debris and the index to be evaluated.


CONCLUSIONS
Strict and precise elimination of debris and aggregates is required. Moreover, strategies and efforts that must be applied to automated elimination of these unwanted elements must be a direct function of the bias they introduce into DNA ploidy measurements.
Boudry
C
C
Pôle Traitement et Analyse d'Images de Basse-Normandie, F. Baclesse Cancer Center, Caen, France.
Herlin
P
P
Coster
M
M
Sola
B
B
Chermant
J L
JL
D000368:Aged; D000369:Aged, 80 and over; D016477:Artifacts; D001932:Brain Neoplasms / Q000737:chemistry* / Q000473:pathology; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D002467:Cell Nucleus / Q000737:chemistry / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D011003:Ploidies
D004273:DNA, Neoplasm