Hen egg-white lysozyme inhibits biological activities of lipopolysaccharides from periodontopathic bacteria.
Lysozyme has a bactericidal activity for some strains of Gram-positive bacteria, by enzymatic cleavage of peptidoglycans that constitute the cell wall. Hen egg-white lysozyme (HEL) was tested in vitro for effects on biological activities of lipopolysaccharides from periodontopathic Gram-negative bacteria. Actinobacillus actinomycetemcomitans, Prevotella intermedia and Porphyromonas gingivalis. HEL inhibited a wide range of activities of these lipopolysaccharides, including activation of Limulus amoebocyte lysate, stimulation of human leukocytes to secrete tumour necrosis factor-alpha, polyclonal activation of mouse B cells, and promotion of osteoclastic differentiation in mouse bone marrow cultures. The anti-endotoxic activity of HEL may be worthy of being intended for periodontal therapy.
Ito
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Department of Biochemistry, Faculty of Dentistry, Kyushu University, Fukuoka, Japan. hitoded@mbox.nc.kyushu-u.ac.jp
Hirata
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Koga
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D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry; D000818:Animals; D000992:Antitoxins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002645:Chickens; D004527:Egg Proteins / Q000378:metabolism* / Q000494:pharmacology; D004529:Egg White; D006090:Gram-Negative Bacteria / Q000737:chemistry*; D006801:Humans; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D009113:Muramidase / Q000378:metabolism* / Q000494:pharmacology; D010010:Osteoclasts / Q000187:drug effects; D010510:Periodontal Diseases / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000737:chemistry; D018713:Prevotella intermedia / Q000737:chemistry; D011485:Protein Binding
D000992:Antitoxins; D004527:Egg Proteins; D008070:Lipopolysaccharides; D009113:Muramidase