title: Interplay of four idiotypes and interaction with autoantibodies in lupus patients, their relatives and their spouses.
abstract: Our aim was to investigate links between systemic lupus erythematosus (SLE)-associated autoantibodies, idiotypes (Id) and genetic predisposition to their development. We studied four public Ids (16/6, WRI 176 beta, RT72 and RT84), identified the Km and Gm phenotypes and sought six selected autoantibodies in 32 SLE patients, 174 of their relatives and 15 spouses. Though anti-double-stranded DNA antibody was uncommon in the relatives (9%), the range of antinuclear reactivities was as broad in the relatives as in the probands. Antibodies to the synthetic peptide U1-RNP-A 35-38 were found in 56% of the patients, 28% of their relatives and 20% of the spouses, whereas antibodies to the Golgi apparatus was present in 7% of the patients, 26% of their relatives and 33% of the spouses. However, most of these family members were unaffected. RT84 Id was positively associated with antibodies to Sm-D peptide 1-20 and to Ro/SSA 60 kD peptide 304-324, but negatively associated with anti-dsDNA activity. The median of age was significantly lower in the RT84 Id-positive than in the RT84 Id-negative individuals. These data suggest that genetic as well as environmental factors are involved in the aetiology of SLE. In addition, RT84-carrying immunoglobulins (Ab2) might be directed to one of many cross-reactive Ids of dsDNA-binding antibodies (Ab1), perhaps down-regulating their production.
authors.affiliation: Brest University Medical School, France.
authors.forename: P
authors.initials: P
authors.lastname: Youinou
authors.forename: D A
authors.initials: DA
authors.lastname: Isenberg
authors.forename: J K
authors.initials: JK
authors.lastname: Kalsi
authors.forename: J M
authors.initials: JM
authors.lastname: Dugoujon
authors.forename: C T
authors.initials: CT
authors.lastname: Ravirajan
authors.forename: S
authors.initials: S
authors.lastname: Muller
authors.forename: F
authors.initials: F
authors.lastname: Blanco
authors.forename: J C
authors.initials: JC
authors.lastname: Piette
authors.forename: L
authors.initials: L
authors.lastname: Guillevin
authors.forename: J
authors.initials: J
authors.lastname: Jouquan
authors.forename: G
authors.initials: G
authors.lastname: Semana
authors.forename: D
authors.initials: D
authors.lastname: Salmon
authors.forename: Y
authors.initials: Y
authors.lastname: Shoenfeld
authors.forename: J F
authors.initials: JF
authors.lastname: Bach
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000974:Antibodies, Antinuclear / Q000097:blood / Q000235:genetics; D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000097:blood / Q000235:genetics*; D001324:Autoantigens; D003429:Cross Reactions; D005190:Family; D005260:Female; D006056:Golgi Apparatus / Q000276:immunology; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000097:blood / Q000235:genetics*; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology*; D008297:Male; D008393:Marriage; D008875:Middle Aged; D010375:Pedigree; D010446:Peptide Fragments / Q000276:immunology; D020733:RNA, Small Cytoplasmic; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000276:immunology; D012261:Ribonucleoproteins; D000099207:SS-A Antigen
chemical_list: D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001324:Autoantigens; D007126:Immunoglobulin Allotypes; D007130:Immunoglobulin Idiotypes; D010446:Peptide Fragments; D020733:RNA, Small Cytoplasmic; D017412:Ribonucleoprotein, U1 Small Nuclear; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human