title: Two limonoids from Turraea obtusifolia (Meliaceae), prieurianin and rohitukin, antagonise 20-hydroxyecdysone action in a Drosophila cell line.
abstract: Bioassay-assisted HPLC analyses yielded two prieurianin-type limonoids, prieurianin and rohitukin, from the seeds of Turraea obtusifolia, which act as antagonists of 20-hydroxyecdysone action in the Drosophila melanogaster BII cell line. With a 20-hydroxyecdysone concentration of 5 x 10(-8) M, the ED50 values for prieurianin and rohitukin are 10(-5) M and 1.25 x 10(-4) M, respectively.
authors.affiliation: Department of Biological Sciences, University of Exeter, Washington Singer Laboratories, Devon, UK.
authors.forename: S D
authors.initials: SD
authors.lastname: Sarker
authors.forename: T
authors.initials: T
authors.lastname: Savchenko
authors.forename: P
authors.initials: P
authors.lastname: Whiting
authors.forename: V
authors.initials: V
authors.lastname: Sik
authors.forename: L
authors.initials: L
authors.lastname: Dinan
mesh_terms: D000818:Animals; D002460:Cell Line; D004331:Drosophila melanogaster; D004441:Ecdysterone / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D036701:Limonins; D015394:Molecular Structure; D010936:Plant Extracts; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D036701:Limonins; D010936:Plant Extracts; D014315:Triterpenes; D004441:Ecdysterone; C105838:prieurianin; C105839:rohitukin