Dog-DAT: a direct agglutination test using stabilized, freeze-dried antigen for the serodiagnosis of canine visceral leishmaniasis.
We have evaluated the use of an improved direct agglutination test (DAT) based on stable, freeze-dried antigen for the detection of anti-Leishmania antibodies in canine serum samples. With a cut-off value of 1:640, the sensitivity of the DAT was shown to be 100% and the specificity of the test was 98.8%.
Oskam
L
L
Royal Tropical Institute, Department of Biomedical Research, Amsterdam, Netherlands. bo@mail.kit.support.nl
Slappendel
R J
RJ
Beijer
E G
EG
Kroon
N C
NC
van Ingen
C W
CW
Ozensoy
S
S
Ozbel
Y
Y
Terpstra
W J
WJ
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D005069:Evaluation Studies as Topic; D005612:Freeze Drying; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D009426:Netherlands / Q000453:epidemiology; D012680:Sensitivity and Specificity; D014421:Turkey / Q000453:epidemiology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan