title: Gene structure and promoter for Adh3 encoding mouse class IV alcohol dehydrogenase (retinol dehydrogenase).
abstract: Class IV alcohol dehydrogenase (ADH) has been shown to function in vitro as a retinol dehydrogenase catalyzing the synthesis of retinoic acid, a pleiotropic gene regulator. To enable genetic studies on the function of this enzyme and regulation of its gene, we have screened a genomic library and isolated the mouse class IV ADH gene (Adh3). The complete mouse class IV ADH coding region was found in nine exons spanning a 14-kb region. Primer extension analysis was used to map the transcription initiation site to a position lying 30 bp upstream of the ATG translation start codon. Nucleotide sequence analysis of the promoter region indicated an absence of both TATA-box and GC-box sequences; this distinguishes it from the promoters for class I, II, and III ADH genes. Sequence comparison of the mouse and human class IV ADH promoters indicated that they share a conserved region located 125-145 bp upstream of the coding region containing adjacent sequences matching the consensus binding sites for transcription factors AP-1 and C/EBP.
authors.affiliation: Burnham Institute, La Jolla, California 92037, USA.
authors.forename: M
authors.initials: M
authors.lastname: Zgombić-Knight
authors.forename: L
authors.initials: L
authors.lastname: Deltour
authors.forename: R J
authors.initials: RJ
authors.lastname: Haselbeck
authors.forename: M H
authors.initials: MH
authors.lastname: Foglio
authors.forename: G
authors.initials: G
authors.lastname: Duester
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D016384:Consensus Sequence; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013045:Species Specificity; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D017931:DNA Primers; D014157:Transcription Factors; D004247:DNA; D000429:Alcohol Oxidoreductases; C022520:retinol dehydrogenase