[Expression of HGF and c-met/HGF receptor mRNA in brains of normal rats and in phaeochromocytoma cells PC-12 of rats treated with NGF].
Gene expression of HGF and c-met proto-oncogene was examined during rat brain development and in cultured PC-12 cells, using reverse-transcriptase (RT)-polymerase chain reaction (PCR) technique. The both mRNAs of HGF and c-met proto-oncogene were remained at low levels in the middle and late stages of gestation (E-13 and E-18). After birth, and the level of both mRNA expression suddenly increased. During P-1 and P-12, their high level of expression continued and then decreased in P-20 and adult brain. Both HGF mRNA and c-met photo-oncogene mRNA were transiently expressed between day 2 and day 5, and disappeared in cultured PC-12 cells treated with NGF. The neurites of PC-12 cells that were treated with anti-sense oligonucleotides of HGF and c-met proto-oncogene, were shorter and fewer in number than untreated control cells. We conclude that neurite extension of PC-12 cells treated with NGF may ensue by way of c-MET protein activation and signal transduction pathways. Thus, c-MET protein activation and up-regulation of the two mRNAs may also play an important role in neuronal maturation in the developing rat brain.
Ukita
H
H
Neuropathology Research Group, Hokuto Hospital, Hokkaido, Japan.
Sawa
H
H
Murakami
H
H
Satone
A
A
Kamada
H
H
Saito
I
I
D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010673:Pheochromocytoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016133:Polymerase Chain Reaction; D019859:Proto-Oncogene Proteins c-met; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D009414:Nerve Growth Factors; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met; D020794:Receptor Protein-Tyrosine Kinases