Identification of a receptor-like protein kinase gene rapidly induced by abscisic acid, dehydration, high salt, and cold treatments in Arabidopsis thaliana.
A cDNA clone for a receptor-like protein kinase gene (RPK1) was isolated from Arabidopsis thaliana. The clone is 1952 bp long with 1623 bp of an open reading frame encoding a peptide of 540 amino acids. The deduced peptide (RPK1) contains four distinctive domains characteristic of receptor kinases: (a) a putative amino-terminal signal sequence domain; (b) a domain with five extracellular leucine-rich repeat sequences; (c) a membrane-spanning domain; and (d) a cytoplasmic protein kinase domain that contains all of the 11 subdomains conserved among protein kinases. The RPK1 gene is expressed in flowers, stems, leaves, and roots. Expression of the RPK1 gene is induced within 1 h after treatment with abscisic acid (ABA). The gene is also rapidly induced by several environmental stresses such as dehydration, high salt, and low temperature, suggesting that the gene is involved in a general stress response. The dehydration-induced expression is not impaired in aba-1, abi1-1, abi2-1, and abi3-1 mutants, suggesting that the dehydration-induced expression of the RPK1 gene is ABA-independent. A possible role of this gene in the signal transduction pathway of ABA and the environmental stresses is discussed.
Hong
S W
SW
Department of Life Science, School of Environmental Engineering, Pohang University of Science and Technology, Kyungbuk, South Korea.
Jon
J H
JH
Kwak
J M
JM
Nam
H G
HG
D000040:Abscisic Acid / Q000494:pharmacology*; D000064:Acclimatization; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary; D003890:Desiccation; D004790:Enzyme Induction; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007700:Kinetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009994:Osmolar Concentration; D018515:Plant Leaves; D018517:Plant Roots; D018547:Plant Stems; D011494:Protein Kinases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D011994:Recombinant Proteins; D000040:Abscisic Acid; D011494:Protein Kinases; C105762:RPK1 protein, Arabidopsis