High-performance liquid chromatographic determination of some amino acids after derivatization with 2-hydroxy-1-naphthaldehyde.
2-Hydroxy-1-naphthaldehyde (HNA) was investigated as a derivatizing reagent for the fluorescence detection of histidine, methionine and tryptophan at 385 and 265 nm. The separation of HNA-derivatized amino acids on a conventional reversed-phase column was achieved in less than 40 min. This method was compared with the reported o-phthaldialdehyde (OPA) method. The procedure was successfully applied to the assay of the pure amino acids in a mixture and their pharmaceutical dosage forms.
el-Brashy
A M
AM
Department of Chemistry, Girls College of Science, Dammam, Kingdom of Saudi Arabia.
al-Ghannam
S M
SM
D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009281:Naphthalenes
D000596:Amino Acids; D007202:Indicators and Reagents; D009281:Naphthalenes; C062247:2-hydroxy-1-naphthaldehyde