Capillary permeability-increasing enzyme-2 from the venom of Agkistrodon caliginosus (kankoku-mamushi): activity resulting from the release of peptides from fibrinogen.
When a mixture of the purified capillary permeability-increasing enzyme-2 from agkistrodon caliginosus venom and rabbit plasma was injected into the depilated skin of the back of rabbits, the capillary permeability-increasing activity was much greater than that induced by injection of the enzyme alone. To isolate the active substance, the mixture was subjected to reversed-phase high-performance liquid chromatography. Among the active peptides, five peptides were purified and their amino acid sequences were analyzed. These peptides were derived from the amino-terminal portion of the B beta-chain of fibrinogen. After the enzyme was incubated with rabbit fibrinogen, the three peptides were purified from the incubation mixture by high-performance liquid chromatography, and their amino acid compositions and amino acid sequences were determined. From these results, it was determined the peptides were derived from the amino-terminal portion of the B beta-chain of fibrinogen. This result indicates that the increase in capillary permeability induced by the enzyme is due to the peptides released from rabbit fibrinogen.
Shimokawa
K
K
Division of Chemistry of Hygiene, Meiji College of Pharmacy, Tokyo, Japan.
Takahashi
H
H
D017836:Agkistrodon; D000595:Amino Acid Sequence; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000378:metabolism* / Q000633:toxicity; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000378:metabolism* / Q000633:toxicity
D003435:Crotalid Venoms; D005340:Fibrinogen; D012697:Serine Endopeptidases; C085657:capillary permeability-increasing enzyme 2