title: Enantioselective determination of FCE 28833, a new potential antiischemic agent, in gerbil plasma using column switching HPLC with UV detection.
abstract: A sensitive and selective high performance liquid chromatographic method using an automated column switching technique for the determination of FCE 28833 enantiomers in gerbil plasma was developed. After solid-liquid extraction using a Supelcosil C18 cartridge, FCE 28833 was eluted on a clean-up column (Spherisorb CN) and the enantiomers were separated using an analytical chiral column (Crownpack CR(+)). The mobile phase (15% methanol in HClO4 1 mM) was directed through the columns at a flow rate of 1 ml/min and the fraction eluted between 13 and 40 min was transferred from the clean-up column into the analytical column. FCE 28833 enantiomers were monitored at 257 nm. The limit of quantitation of the method was 20 ng/ml plasma for both enantiomers and proved to be linear, precise, and accurate for the assay of both enantiomers in the 20-6,000 ng/ml concentration range. No interference from the blank gerbil plasma sample was observed. The suitability of the method was assessed using plasma samples obtained from male gerbils treated with a single oral dose (400 mg/kg) of FCE 28833.
authors.affiliation: Department of Pharmacokinetics and Metabolism, R&D, Pharmacia & Upjohn, Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Breda
authors.forename: S
authors.initials: S
authors.lastname: Sarati
authors.forename: G
authors.initials: G
authors.lastname: Basileo
authors.forename: P
authors.initials: P
authors.lastname: Dostert
mesh_terms: D000818:Animals; D002087:Butyrates / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005849:Gerbillinae; D008297:Male; D018696:Neuroprotective Agents / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D002087:Butyrates; C104254:FCE 28833; D018696:Neuroprotective Agents