Evidence for two types of nicotinic receptors in the cat carotid body chemoreceptor cells.
Current concepts on the location and functional significance of nicotinic receptors in the carotid body rest on alpha-bungarotoxin binding and autoradiographic studies. Using an in vitro preparation of the cat carotid body whose catecholamine deposits have been labeled by prior incubation with the tritiated natural precursor [3H]tyrosine, we have found that nicotine induces release of [3H]catecholamines in a dose-dependent manner (IC50 = 9.81 microM). We also found that mecamylamine (50 microM) completely abolished the nicotine-induced release, while alpha-bungarotoxin (100 nM; approximately 20 times its binding Kd) only reduced the release by 56%. These findings indicate that chemoreceptor cells, and perhaps other carotid body structures, contain nicotinic receptors that are not sensitive to alpha-bungarotoxin and force a revision of the current concepts on cholinergic mechanisms in the carotid body chemoreception.
Obeso
A
A
Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de Medicina, Universidad de Valladolid, Spain.
Gómez-Niño
M A
MA
Almaraz
L
L
Dinger
B
B
Fidone
S
S
González
C
C
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D002344:Carotid Body / Q000502:physiology*; D002395:Catecholamines / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D002415:Cats; D002628:Chemoreceptor Cells / Q000502:physiology*; D066298:In Vitro Techniques; D007700:Kinetics; D008464:Mecamylamine / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009538:Nicotine / Q000494:pharmacology*; D011978:Receptors, Nicotinic / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D014316:Tritium; D014443:Tyrosine / Q000378:metabolism
D002038:Bungarotoxins; D002395:Catecholamines; D002396:Catechols; D011978:Receptors, Nicotinic; D014316:Tritium; D015102:3,4-Dihydroxyphenylacetic Acid; D014443:Tyrosine; D008464:Mecamylamine; D009538:Nicotine; C034221:catechol