title: Binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin to AH receptor in placentas from normal versus abnormal pregnancy outcomes.
abstract: Binding of [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin to AH receptor was characterized in cytosol from human placentas in which the pregnancy outcome was normal compared with pregnancies in which there was some adverse outcome (premature birth; intrauterine growth retardation; structural abnormality). No significant difference was detected between normal and adverse outcomes in the concentration of AH receptor sites (Bmax) nor in the affinity with which [3H]TCDD bound to the receptor (Kd). Aryl hydrocarbon hydroxylase activity, a CYP1A1 enzyme regulated by the AH receptor, was elevated in placental microsomes from smokers; this elevation was associated with intrauterine growth retardation.
authors.affiliation: Department of Pharmacology, University of Toronto, Ontario, Canada.
authors.forename: A B
authors.initials: AB
authors.lastname: Okey
authors.forename: J V
authors.initials: JV
authors.lastname: Giannone
authors.forename: W
authors.initials: W
authors.lastname: Smart
authors.forename: J M
authors.initials: JM
authors.lastname: Wong
authors.forename: D K
authors.initials: DK
authors.lastname: Manchester
authors.forename: N B
authors.initials: NB
authors.lastname: Parker
authors.forename: M M
authors.initials: MM
authors.lastname: Feeley
authors.forename: D L
authors.initials: DL
authors.lastname: Grant
authors.forename: A
authors.initials: A
authors.lastname: Gilman
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D000013:Congenital Abnormalities / Q000209:etiology / Q000378:metabolism; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology / Q000378:metabolism; D006801:Humans; D007752:Obstetric Labor, Premature / Q000209:etiology / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D011248:Pregnancy Complications / Q000209:etiology / Q000378:metabolism*; D011256:Pregnancy Outcome; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
chemical_list: D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D001189:Aryl Hydrocarbon Hydroxylases