title: Metabolism of platelet-activating factor in rat epididymal spermatozoa.
abstract: A role for platelet-activating factor (PAF) in sperm function has been proposed. In the present investigation the metabolism of PAF was examined in sperm and epididymal tissue. The major regulatory enzymes for the synthesis of PAF via the de novo pathway have been established in spermatozoa; these include acetyltransferase and cholinephosphotransferase specific for PAF biosynthesis. De novo acetyltransferase activity for PAF biosynthesis in spermatozoa was significantly higher than that of the acetyltransferase of the remodeling pathway. A metabolic pathway was described for the catabolism of PAF in sperm, involving PAF-acetylhydrolase (-AH), lysophospholipase D, and a phosphohydrolase. An isozyme of PAF-AH similar to that reported in sera was also demonstrated in epididymal fluid and tissue. This isozyme was distinctly different from that found in the spermatozoa. The partial inactivation of PAF-AH by the vaginal pH, and/or its detachment from sperm during migration to the site of fertilization, may allow increased motility and migration to the site of fertilization. It is suggested that a decapacitation factor previously described may be related to PAF-AH.
authors.affiliation: Department of Biochemistry, The Cecil H. and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical Center at Dallas, 75235-9051, USA.
authors.forename: K
authors.initials: K
authors.lastname: Muguruma
authors.forename: J M
authors.initials: JM
authors.lastname: Johnston
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D004822:Epididymis / Q000166:cytology*; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D010741:Phospholipases A / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D013094:Spermatozoa / Q000378:metabolism*; D014316:Tritium; D000099189:Lysophospholipase D
chemical_list: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000123:Acetyltransferases; D002798:Diacylglycerol Cholinephosphotransferase; D007527:Isoenzymes; D010741:Phospholipases A; D010727:Phosphoric Diester Hydrolases; D010972:Platelet Activating Factor; D014316:Tritium; D000099189:Lysophospholipase D; C029271:O-deacetyl platelet activating factor; C046035:1-alkylglycerophosphocholine acetyltransferase