title: Vectors for inducible expression of toxic gene products in bloodstream and procyclic Trypanosoma brucei.
abstract: We previously described a system for exogenous control of gene expression in procyclic trypanosomes which depends upon the binding of a tetracycline-inducible repressor to operators situated at the transcriptional start site of the PARP promoter. The recombinant constructs are introduced into non-transcribed spacers of the ribosomal RNA repeat, in an orientation opposite to that of rRNA transcription. Using this system, gene expression could be regulated over four orders of magnitude, but it was not possible to express toxic gene products because selection of recombinant trypanosomes depended on the activity of the inducible promoter. We describe here the characteristics of vectors that include two promoters: a tetracycline-inducible one to drive expression of the toxic products, and a constitutive one to drive transcription of the selectable marker. Relatively high levels of non-induced (non-tetracycline-dependent) expression were seen in some trypanosome clones; this was not usually due to read-through of multiple tandemly-integrated plasmids or tet operator mutations. A variety of constructs differing in resistance marker, 3'-untranslated region (3'-UTR) and the nature of the constitutive promoter was tested. Vectors allowing the successful expression of toxic and other genes in both life cycle stages with regulation factors of up to 700 fold were obtained.
authors.affiliation: Zentrum für Molekular Biologie, Heidelberg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Biebinger
authors.forename: L E
authors.initials: LE
authors.lastname: Wirtz
authors.forename: P
authors.initials: P
authors.lastname: Lorenz
authors.forename: C
authors.initials: C
authors.lastname: Clayton
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D004275:DNA, Ribosomal / Q000235:genetics; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005819:Genetic Markers; D005822:Genetic Vectors; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D015800:Protozoan Proteins; D014118:Toxins, Biological / Q000096:biosynthesis* / Q000235:genetics; D014346:Trypanosoma brucei brucei / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D005819:Genetic Markers; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D014118:Toxins, Biological; C053277:procyclic acidic repetitive protein, Trypanosoma; D008156:Luciferases