title: Paracrine effect of human vascular endothelial cells on human vascular smooth muscle cell proliferation: transmembrane co-culture method.
abstract: To investigate the role endothelial cells have on underlying smooth muscle cell proliferation, human aortic vascular smooth muscle cells were co-cultured with human aortic endothelial cells at different cell densities, using a transmembrane co-culture method. Subconfluent endothelial cells subseeded at low (0.5 x 10(4) cells/well) and medium densities (2.0 x 10(4) cells/well) stimulated smooth muscle cell proliferation by 43 +/- 14% (P < 0.01) and 39 +/- 8% (P < 0.02), respectively. However, this stimulatory effect on smooth muscle cell proliferation was not evident in confluent endothelial cells subseeded at high cell density (8.0 x 10(4) cells/well). Treatment of smooth muscle cells with trapidil, at 10(-6) M, for anti-platelet derived growth factor (PDGF) effect or with endothelin-1 receptor blocker FR 139317, at 10(-6) M failed to inhibit this stimulatory effect. These results imply that subconfluent human endothelial cells are able to exert a stimulatory effect on human smooth muscle cell proliferation, and that this endothelial paracrine growth effect may not be mediated by endothelin or PDGF.
authors.affiliation: Second Department of Internal Medicine, Iwate Medical University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: S
authors.initials: S
authors.lastname: Makita
authors.forename: K
authors.initials: K
authors.lastname: Hiramori
mesh_terms: D001011:Aorta; D001381:Azepines / Q000378:metabolism / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods; D015194:Diffusion Chambers, Culture; D065128:Endothelin Receptor Antagonists; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D007211:Indoles / Q000378:metabolism / Q000494:pharmacology; D008567:Membranes, Artificial; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D014192:Trapidil / Q000378:metabolism / Q000494:pharmacology
chemical_list: D001381:Azepines; D065128:Endothelin Receptor Antagonists; D007211:Indoles; D008567:Membranes, Artificial; C079574:FR 139317; D014192:Trapidil