A root-specific iron-regulated gene of tomato encodes a lysyl-tRNA-synthetase-like protein.
The tomato mutant chloronerva exhibits a defect in iron-uptake regulation. Despite high apoplastic and symplastic iron concentrations, the mutant shows characteristic symptoms of iron deficiency. Using a subtractive-hybridisation approach, we have screened for cDNA clones specific for genes with altered expression in wild-type versus mutant root tissue. Based on this clone collection, we have isolated and characterised a 2075-bp full-length cDNA encoding a lysyl-tRNA-synthetase-like protein. The corresponding gene is localised as a single copy on chromosome 10. Its expression is strongly induced by changes in the iron status of the plant. This iron-dependent regulation is superimposed upon a strict root specificity of gene expression. Possible functions of the gene product other than in protein biosynthesis will be discussed.
Giritch
A
A
Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben,Germany.
Herbik
A
A
Balzer
H J
HJ
Ganal
M
M
Stephan
U W
UW
Bäumlein
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001693:Biological Transport, Active / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005784:Gene Amplification; D015870:Gene Expression; D017343:Genes, Plant; D006801:Humans; D007501:Iron / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018744:DNA, Plant; D007501:Iron; D008250:Lysine-tRNA Ligase