[Anterior chamber inflammation after the injection of endothelin-1 into the vitreous and the effect of ETA receptor antagonist].
To assess the receptors which mediate the inflammatory reaction induced by endothelin-1 (ET-1), we investigated the influence of pre-treatment with an ETA receptor antagonist (97-139) on the increase of aqueous protein concentration (APC) after the injection of ET-1 (10(-4), 10(-5)M) into the vitreous cavity of pigmented rabbits. The concentration of prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) in the aqueous humor after the injection of 10(-4)M ET-1 with or without pre-treatment with 97-139 (10(-1), 10(-2), 10(-3)M) was also studied. Pre-treatment with 10(-2)M and 10(-1)M 97-139 completely prevented the APC increase induced by 10(-5)M and 10(-4)M ET-1, respectively. Increases in aqueous PGE2 concentration were observed after the injection of ET-1, which was inhibited by pre-treatment with 97-139. Aqueous LTB4 concentration was not changed significantly by ET-1. These results indicate that the effects of ET-1 on APC are at least partially mediated by the cyclooxygenase pathway of arachidonic acid cascade, and that ETA receptors play an important role in these reactions.
Shoji
N
N
Department of Ophthalmology, Musashino Red Cross Hospital, Tokyo, Japan.
Oshika
T
T
Masuda
K
K
D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects*; D001082:Aqueous Humor / Q000737:chemistry; D002109:Caffeic Acids / Q000494:pharmacology*; D015232:Dinoprostone / Q000032:analysis; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1 / Q000494:pharmacology*; D005260:Female; D007249:Inflammation / Q000139:chemically induced; D007975:Leukotriene B4 / Q000032:analysis; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D011817:Rabbits; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin / Q000502:physiology; D014822:Vitreous Body / Q000187:drug effects*
D002109:Caffeic Acids; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin; C086421:S 0139; D007975:Leukotriene B4; D009828:Oleanolic Acid; D015232:Dinoprostone