title: An investigation into the potential of extracellular matrix factors for attachment and proliferation of human keratinocytes on skin substitutes.
abstract: The purpose of this investigation was to assess the potential of commercially available extracellular matrix proteins, as enhancers of human keratinocyte attachment and proliferation, with a view to their incorporation into a skin equivalent. The following substrates were studied: type I and type IV collagen, fibronectin, gelatin and laminin. Human keratinocytes were cultured in low-calcium, serum-free medium. The number of cells attached to each substrate, observed under phase-contrast inverted microscopy in randomly selected fields of view, were counted 2 h afterseeding. Measurements of growth rate and colony-forming efficiency were made at 24-h intervals. None of the substrates tested were found to have an effect significant enough to warrant further investigation or inclusion into skin equivalent.
authors.affiliation: Yorkshire Regional Tissue Bank, Pinderfields General Hospital, Wakefield, UK.
authors.forename: J T
authors.initials: JT
authors.lastname: Daniels
authors.forename: J N
authors.initials: JN
authors.lastname: Kearney
authors.forename: E
authors.initials: E
authors.lastname: Ingham
mesh_terms: D000704:Analysis of Variance; D002056:Burns / Q000601:surgery*; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000627:therapeutic use; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism / Q000627:therapeutic use*; D005353:Fibronectins / Q000627:therapeutic use; D005780:Gelatin / Q000627:therapeutic use; D006133:Growth Substances / Q000378:metabolism / Q000627:therapeutic use*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D007797:Laminin / Q000627:therapeutic use; D016038:Skin Transplantation / Q000379:methods; D017768:Skin, Artificial
chemical_list: D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006133:Growth Substances; D007797:Laminin; D005780:Gelatin; D003094:Collagen