Production of a T-cell clone which reacts with membrane proteins of Acholeplasma laidlawii.
The role of cellular immunity in mycoplasma infection is not completely understood. In this study, we established mycoplasma-specific T-cell clones to evaluate cellular immunity in mycoplasma infection. We developed a T-cell clone (G-10) which was stimulated with Acholeplasma laidlawii. The T-cell clone G-10, CD4+ and T-cell receptor (TCR) alpha beta- recognized the 42- and 65-kilodalton (kDa) membrane proteins of A. laidlawii and responded to A. hippikon. Hence, the application of mycoplasma-specific T cells such as G-10 in animal models may allow the assessment of cellular immune response to mycoplasma infection.
Kuwano
K
K
Department of Microbiology, Kurume University School of Medicine, Japan.
Ono
S
S
Akashi
A
A
Ohishi
M
M
Shigematsu
H
H
Arai
S
S
D000128:Acholeplasma laidlawii / Q000276:immunology*; D000818:Animals; D000938:Antigen-Presenting Cells; D001426:Bacterial Proteins / Q000276:immunology*; D002999:Clone Cells; D003429:Cross Reactions; D006183:H-2 Antigens; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D001426:Bacterial Proteins; D006183:H-2 Antigens; D008565:Membrane Proteins