Immunological characterization of a neuronal antibody (anti-Tr) associated with paraneoplastic cerebellar degeneration and Hodgkin's disease.
We studied an autoantibody (called anti-Tr), found in the serum and CSF of five patients with paraneoplastic cerebellar degeneration (PCD) and Hodgkin's disease (HD). Anti-Tr antibodies labelled the cytoplasm of Purkinje cells of human and rat cerebellum. The molecular layer of rat cerebellum showed a characteristic dotted pattern suggestive of immunoreactivity of dendritic spines of Purkinje cells. Patients with cerebellar disorders without HD (159) or HD without PCD (30) did not harbor anti-Tr antibodies. Immunoblots of human Purkinje cells or rat and mouse cerebellum were negative. Anti-Tr antibodies, as defined in this study, appear specific for HD-associated PCD. The immunohistochemical pattern described in the rat cerebellum coupled with the absence of reactivity in the immunoblot may be used to identify anti-Tr antibodies.
Graus
F
F
Service of Neurology, Hospital Clínic i Provincial, Barcelona, Spain.
Dalmau
J
J
Valldeoriola
F
F
Ferrer
I
I
Reñe
R
R
Marin
C
C
Vecht
C J
CJ
Arbizu
T
T
Targa
C
C
Moll
J W
JW
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000134:cerebrospinal fluid / Q000276:immunology*; D002526:Cerebellar Diseases / Q000150:complications / Q000276:immunology*; D005260:Female; D006689:Hodgkin Disease / Q000134:cerebrospinal fluid / Q000150:complications / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009410:Nerve Degeneration; D009474:Neurons / Q000276:immunology*; D010257:Paraneoplastic Syndromes / Q000134:cerebrospinal fluid / Q000150:complications / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D000906:Antibodies