Two pathways of the nitric oxide-induced cytotoxycal action.
Nitric oxide is a diffusible messenger with multiple biological functions. We show here that NO-generating compound, S-nitrosoglutathione (GSNO) induces apoptosis in human chondrocytes and causes necrosis-like cell death in human epithelial CaOv cell line. Pretreatment of chondrocytes with low-dose GSNO or with gamma-interferon enhances their tolerance to the second high-concentration GSNO exposure. On the contrary, in CaOv cells low-dose GSNO pretreatment diminishes the resistance and increases cytolysis at the second GSNO exposure. We conclude that human chondrocytes possess specific and inducible mechanism preventing cell killing by nitric oxide.
Turpaev
K T
KT
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. turpaev@imb.imb.ac.ru
Amchenkova
A M
AM
Narovlyansky
A N
AN
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D004247:DNA / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis; D005978:Glutathione / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000633:toxicity; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D009336:Necrosis; D009569:Nitric Oxide / Q000493:pharmacokinetics / Q000633:toxicity*; D009603:Nitroso Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D011994:Recombinant Proteins; D026422:S-Nitrosoglutathione
D009603:Nitroso Compounds; D011994:Recombinant Proteins; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D007371:Interferon-gamma; D004247:DNA; D005978:Glutathione