Ozone-exposure depletes vitamin E and induces lipid peroxidation in murine stratum corneum.
The presence of ozone (O(3)) in photochemical smog is an important health concern. We hypothesized that the stratum corneum (SC), as the outermost skin layer and the permeability barrier of the skin, represents a sensitive target for O(3)-induced oxidative stress. To test this hypothesis, SKH-1 hairless mice were anesthetized and exposed for 2 h to O(3) by using two strategies: (i) single exposures to 0 (n = 12), 1 (n = 4), 5 (n = 4), and 10 (n = 4) ppm; and (ii) repeated daily exposures to 0 ppm (controls; n = 4) and 1 ppm (n = 4) for six consecutive days. New techniques based on the removal of SC by tape stripping were used to analyze the biologic effects of O(3) with respect to vitamin E depletion and lipid peroxidation. SC tissue was extracted from the tape and immediately analyzed by HPLC for vitamin E and malondialdehyde (MDA) concentrations. After in vivo exposure to increasing O(3) doses, vitamin E was depleted and MDA formation was increased, both in a dose-dependent manner. Remarkably, repeated low-level O(3) exposures resulted in cumulative oxidative effects in the SC: As compared with O(3) exposures of 0 ppm (alpha-tocopherol, 8.95 +/- 1.3 pmol per mg; gamma-tocopherol, 3.00 +/- 0.3 pmol per mg; MDA, 3.69 +/- 0.3 pmol per mg), vitamin E was depleted (alpha-tocopherol, 2.90 +/- 0.6 pmol per mg, p < 0.001; gamma-tocopherol, 0.5 +/- 0.1 pmol per mg, p < 0.001) and MDA levels were increased (4.5 +/- 0.2; p < 0.01). This report demonstrates the unique susceptibility of the SC to oxidative damage upon exposure to O(3).
Thiele
J J
JJ
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
Traber
M G
MG
Polefka
T G
TG
Cross
C E
CE
Packer
L
L
D000393:Air Pollutants / Q000009:adverse effects*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D008812:Mice, Hairless; D010083:Oxidants, Photochemical / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects; D010126:Ozone / Q000009:adverse effects*; D012867:Skin / Q000737:chemistry / Q000187:drug effects*; D014810:Vitamin E / Q000378:metabolism*
D000393:Air Pollutants; D010083:Oxidants, Photochemical; D014810:Vitamin E; D008315:Malondialdehyde; D010126:Ozone