Aberrant expression and function of p53 in T-cells immortalized by HTLV-I Tax1.
The expression and function of p53 tumor suppressor protein was investigated in T-cells immortalized by the Tax1 protein of HTLV-I. Conformationally wild-type p53 was expressed at elevated levels in Tax1-immortalized T-cells by post-transcriptional mechanisms when compared with normal T-cells. Luciferase assays with a reporter plasmid containing p53-binding sites revealed an impairment in the transactivating function of p53 in Tax1-immortalized T-cells. Our results suggest an important role for Tax1 in the aberrant expression and function of p53 observed in many HTLV-I transformed cells.
Akagi
T
T
Virology Division, National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan.
Ono
H
H
Tsuchida
N
N
Shimotohno
K
K
D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D016356:Gene Products, tax / Q000502:physiology*; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D015533:Transcriptional Activation; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000737:chemistry / Q000502:physiology*
D016356:Gene Products, tax; D016159:Tumor Suppressor Protein p53