Determination of drug susceptibility of Mycobacterium tuberculosis through mycolic acid analysis.
In the present work a rapid method to determine the susceptibility of Mycobacterium tuberculosis to isoniazid and streptomycin by determining levels of mycolic acids by high-performance liquid chromatography (HPLC) was developed. Mycobacterial growth kinetics in the presence and absence of antituberculosis drugs was characterized by evaluating the total area corresponding to mycolic acid peaks (TAMA). Results show a linear relationship between the logarithm of CFU per milliliter and TAMA and show that it is possible to detect growth inhibition of M. tuberculosis in the presence of isoniazid or streptomycin by using HPLC in 3 and 4 days, respectively.
Garza-González
E
E
Departamento de Bioquímica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico.
Guerrero-Olazarán
M
M
Tijerina-Menchaca
R
R
Viader-Salvadó
J M
JM
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D009171:Mycolic Acids / Q000032:analysis*
D000904:Antibiotics, Antitubercular; D009171:Mycolic Acids