cDNA cloning of rat mitochondrial cyclophilin.
Human cyclophilin-3 (hCyp-3) cDNA was used to screen a rat skeletal muscle cDNA library which led to the isolation of a full-length cDNA encoding the rat homologue. The derived amino acid sequence showed 89.5% identity with the human sequence, with major differences being restricted to the mitochondrial targeting sequence. The N-terminal sequence of the purified rat liver mitochondrial cyclophilin (CyP-D) corresponded to that derived from the cDNA following 30 amino acids of targeting sequence. This confirms that hCyp-3 encodes mitochondrial matrix CyP (CyP-D), which plays a crucial role in the mitochondrial permeability transition. CyP-D mRNA of a single sized (1.5 kb) was shown by Northern blotting to be present in liver, heart, skeletal muscle and brain.
Woodfield
K Y
KY
Department of Biochemistry, School of Medical Science, University of Bristol, UK.
Price
N T
NT
Halestrap
A P
AP
D000591:Amino Acid Isomerases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D000081406:Peptidyl-Prolyl Isomerase F; D021983:Cyclophilins; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics*; D008930:Mitochondria, Liver / Q000201:enzymology / Q000235:genetics; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal; D011498:Protein Precursors / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D002352:Carrier Proteins; D000081406:Peptidyl-Prolyl Isomerase F; D018076:DNA, Complementary; D011498:Protein Precursors; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D000591:Amino Acid Isomerases; D021983:Cyclophilins