Determination of the nucleotide conformation in the productive enzyme-substrate complexes of RNA-depolymerases.
The aim of this work is to determine the conformation of the nucleobase adjacent to the cleavable phosphodiester bond in the productive enzyme-substrate complex of RNA-depolymerizing enzymes. To this end the kinetic parameters of hydrolysis of UpA, 2'-C-Me- and 3'-C-Me-UpA were determined for RNase A, RNase Pb2, nuclease S1 and snake venom phosphodiesterase. In these derivatives the ranges of the allowed orientation of uridine residues are restricted due to the substitution of methyl groups for the ribose hydrogen atoms. The results described demonstrate that the proposed method is of general value for the estimation of the nucleotide glycoside angles in the productive enzyme-substrate complexes.
Moiseyev
G P
GP
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Yakovlev
G I
GI
Lysov YuP
Chernyi
A A
AA
Polyakov
K M
KM
Oivanen
M
M
Lönnberg
H
H
Beigelman
L N
LN
Efimtseva
E V
EV
Mikhailov
S N
SN
D000818:Animals; D002151:Calorimetry; D002417:Cattle; D015226:Dinucleoside Phosphates / Q000737:chemistry* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010407:Penicillium / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000378:metabolism; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000737:chemistry* / Q000378:metabolism; D012910:Snake Venoms
D015226:Dinucleoside Phosphates; D012910:Snake Venoms; C009628:uridylyl-(3'-5')-adenosine; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic; D015719:Single-Strand Specific DNA and RNA Endonucleases; D010727:Phosphoric Diester Hydrolases