Characterization of smooth lipopolysaccharides and O polysaccharides of Brucella species by competition binding assays with monoclonal antibodies.
Previously, four epitope specificities on the O chain of Brucella species were reported: M, A, C, and C/Y. In this work, according to monoclonal antibody binding to smooth lipopolysaccharides of Yersinia enterocolitica 0:9, Brucella abortus W99 (A-dominant strain), and B. melitensis Rev1 (M-dominant strain), seven O-chain epitope specificities were defined: M, A, C (M > A), C (M = A), C/Y (M > A), C/Y (M = A) and C/Y (A > M). Competitive binding assays between these monoclonal antibodies suggested that these different epitopes are probably overlapping structures.
Weynants
V
V
Unité d'Immunologie-Microbiologie, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium. Vincent.Weynants@fundp.ac.be
Gilson
D
D
Cloeckaert
A
A
Tibor
A
A
Denoel
P A
PA
Godfroid
F
F
Limet
J N
JN
Letesson
J J
JJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002003:Brucella abortus / Q000276:immunology*; D017347:Brucella melitensis / Q000276:immunology*; D018604:Epitope Mapping; D000939:Epitopes / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008070:Lipopolysaccharides / Q000276:immunology*; D019081:O Antigens / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D019081:O Antigens