title: Prolonged prior infection with Chlamydia prevents adverse pregnancy outcome in a murine model.
abstract: OBJECTIVE
  Our purpose was to compare the rate of adverse pregnancy outcome in pregnant mice with lower genital tract chlamydial infection who had a prior short chlamydial infection versus a prior long-term infection.
  
  
  METHODS
  A total of 127 female mice were divided into short-term and long-term infection groups. We infected the lower genital tracts with Chlamydia trachomatis. After 7 days in the short-term infection group and 30 days in the long-term infection group, we treated the mice with tetracycline-impregnated chow. After documentation of cure, the mice were mated and transvaginally reinfected with Chlamydia trachomatis. Forty-one of the 127 (32%) mice became pregnant. We noted the number of mice with fetal death and the number of pups present. We cultured the lower uterine segment and the pups for Chlamydia.
  
  
  RESULTS
  Seven of 21 (33%) mice in the short-term infection group had fetal deaths compared with 1 of 20 (5%) in the long-term infection group (p < 0.05). In the short-term infection group 21 of 21 (100%) mice had positive transvaginal chlamydial cultures after reinoculation compared with only 7 of 20 (35%) in the long-term infection group (p < 0.000004). Seventeen of 21 (81 %) mice in the short-term infection group had positive chlamydial cultures from the lower uterine segment versus 1 of 20 (5%) in the long-term infection group (p < 0.000001). Sixty-five percent of pups in the short-term infection group and none (0%) of the pups in the long-term infection group were positive for Chlamydia (p < 0.00001).
  
  
  CONCLUSIONS
  We conclude that in this murine model a prior 30-day genital tract infection with Chlamydia protects pregnant mice from subsequent reinfection and adverse pregnancy outcomes.
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Texas Medical School-Houston 77026, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Blanco
authors.forename: T S
authors.initials: TS
authors.lastname: Wen
authors.forename: K
authors.initials: K
authors.lastname: Bishop
mesh_terms: D000818:Animals; D002690:Chlamydia Infections / Q000150:complications; D002692:Chlamydia trachomatis; D005260:Female; D005313:Fetal Death / Q000209:etiology*; D051379:Mice; D008815:Mice, Inbred Strains; D007752:Obstetric Labor, Premature / Q000209:etiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious; D011256:Pregnancy Outcome; D013997:Time Factors