Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression.
The expression patterns of the Ly-6C Ag were examined on splenic and thymic lymphocyte subsets of Ly-6.1 and Ly-6.2 strains of mice using the rat mAb 15.1. Ly-6C is expressed on subsets of CD4+ and CD8+ splenocytes, and a portion of NK cells. Within the splenic and lymph node CD4+ T cell compartment, Ly-6C expression is restricted to Ly-6.2 strains of mice, and is present on a subset of naive cells. Ly-6C is expressed on the majority of peripheral CD8+ T cells in both Ly-6.1 and Ly-6.2 strains, and is found primarily on the Ag-experienced subset. In the thymus, Ly-6C is present on subpopulations of CD4- CD8+, CD4- CD8-, and CD4+ CD8- cells. Ly-6C+ CD4- CD8+ thymocytes show a mature phenotype, while Ly-6C+ CD4- CD8- and Ly-6C+ CD4+ CD8- thymocytes appear to be part of the recently described NK1.1+ alphabeta TCR+ population. On account of the marked differences in Ly-6C expression on peripheral CD4+ T cells from Ly-6.1 and Ly-6.2 strains of mice, additional experiments were undertaken to assess Ly-6C expression in parental and Ly-6.1 x Ly-6.2 F1 mice. Neither phenotype dominated in the F1 offspring, with frequencies of Ly-6C+ CD4+ splenocytes falling in the intermediate range. Further experiments compared the staining patterns of the rat anti-pan Ly-6C (Ly-6.1 and Ly-6.2) Ab with a mouse anti-Ly-6.2 allotype specific Ab, with emphasis on both Ly-6.2 and Ly-6.1 x Ly-6.2 F1 mice. The results demonstrate the presence of lymphocytes that express the pan form of Ly-6C but not the form recognized by the alloantibody. This latter finding suggests the presence of more than one form of the Ly-6C Ag.
Schlueter
A J
AJ
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA.
Malek
T R
TR
Hostetler
C N
CN
Smith
P A
PA
deVries
P
P
Waldschmidt
T J
TJ
D000483:Alleles; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000950:Antigens, Ly / Q000378:metabolism*; D051927:CD24 Antigen; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D000939:Epitopes; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D018960:Hyaluronan Receptors / Q000032:analysis; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology; D008562:Membrane Glycoproteins; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013154:Spleen / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000950:Antigens, Ly; D051927:CD24 Antigen; C497890:Cd24a protein, mouse; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; C032907:Q surface antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2