[Detection of highly conservative domains in the sequence of 17S ribosomal DNA from Tetrahymena pyriformis].
Domains differing in the degree of evolutionary conservatism were revealed within the 17S ribosomal DNA (rDNA) sequence from Tetrahymena pyriformis by means of computer analysis. The method proposed allows evolution of rDNA structure to be studied in eukaryotes. Superconservative regions revealed within the DNA sequence tested can be used to construct universal primers for studying rDNA structure in various taxa by means of polymerase chain reaction (PCR).
Mukha
D V
DV
Sidorenko
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AP
D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D005057:Eukaryotic Cells; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D012984:Software; D013769:Tetrahymena pyriformis / Q000235:genetics*
D016054:DNA, Protozoan; D004275:DNA, Ribosomal