title: Colonic muscle enhances the production of interleukin-1 beta messenger RNA in experimental colitis.
abstract: BACKGROUND
  Inflammatory bowel diseases are accompanied by alterations in the contractile function of colonic muscle. Whether these changes are also accompanied by the production of interleukin-1 beta (IL-1 beta) mRNA remains unknown.
  
  
  OBJECTIVE
  To investigate the profile of IL-1 beta mRNA in the colonic muscle of rats with acetic acid induced colitis.
  
  
  METHODS
  Colitis was induced by intrarectal administration of 1 ml of 4% acetic acid 8 cm from anal margin. Myeloperoxidase activity was used as a marker of inflammation in the colon. RNA was extracted from colonic muscle and was used to amplify the level of IL-1 beta mRNA with a reverse transcription coupled polymerase chain reaction (RT-PCR) method developed in this study.
  
  
  RESULTS
  Colonic muscle exhibited constitutive expression of IL-1 beta mRNA. The level was enhanced significantly, one, two, and five days after acetic acid administration. On day 7 after treatment, the level of IL-1 beta mRNA had returned baseline values. This profile of IL-1 beta mRNA was consistent with myeloperoxidase activity.
  
  
  CONCLUSIONS
  IL-1 beta is a putative mediator of the changes in muscle function in this model of colitis.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Kuwait University.
authors.forename: I
authors.initials: I
authors.lastname: Khan
authors.forename: F M
authors.initials: FM
authors.lastname: al-Awadi
mesh_terms: D019342:Acetic Acid; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D019342:Acetic Acid