title: Cytogenetic analysis of horse oocytes matured in vitro for different periods of time.
abstract: This paper presents the results of recovering horse oocytes by aspiration and maturation in vitro for 24, 30, 36 or 42 h. A total of 522 oocytes were recovered from 221 ovaries (2.4 per ovary) and 271 oocytes (51.9%) were selected for in vitro maturation (IVM). Oocytes were cultured in maturation medium (TCM 199 + estrus cow serum [ECS] + follicle-stimulating hormone [FSH] + 17 beta-estradiol + gentamycin). One hundred and seventy oocytes were cytogenetically analysed (68.3%). Cytogenetic analysis showed that the stage of maturation (first telophase-TI or second metaphase-MII) for fertilization was achieved in 42.2% of oocytes after 24 h, 70.4% of oocytes after 30 h, 75.0% of oocytes after 36 h and 74.4% of oocytes after 42 h of in vitro maturation.
authors.affiliation: Department of Genetics and Animal Breeding, Agricultural University of Poznań, Poland. jarys@jay.au.pozman.pl
authors.forename: J
authors.initials: J
authors.lastname: Sosnowski
authors.forename: D
authors.initials: D
authors.lastname: Lechniak
authors.forename: M
authors.initials: M
authors.lastname: Brzozowska
authors.forename: M
authors.initials: M
authors.lastname: Switoński
mesh_terms: D000818:Animals; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure*; D003470:Culture Media; D004958:Estradiol; D004971:Estrus; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone; D005839:Gentamicins; D006736:Horses / Q000235:genetics*; D008540:Meiosis; D009865:Oocytes / Q000254:growth & development* / Q000648:ultrastructure*; D013997:Time Factors
chemical_list: D003470:Culture Media; D005839:Gentamicins; D004958:Estradiol; D005640:Follicle Stimulating Hormone