Nitric oxide stimulates prostaglandin synthesis in cultured rabbit gastric cells.
Both prostaglandins (PGs) and nitric oxide (NO) have cytoprotective and hyperemic effects in the stomach. However, the effect of NO on PG synthesis in gastric mucosal cells is unclear. We examined whether sodium nitroprusside (SNP), a releaser of NO, stimulates PG synthesis in cultured rabbit gastric mucus-producing cells. These cells did not release NO themselves. Co-incubation with SNP (2 x 10(-4), 5 x 10(-4), 10(-3) M) increased PGE2 synthesis, and SNP (10(-3) M) increased PGI2 synthesis in these cells. Hemoglobin, a scavenger of NO, (10(-5) M) eliminated the increase in PGE2 synthesis by SNP, but methylene blue, an inhibitor of soluble guanylate cyclase, (5 x 10(-5) M) did not affect the increase in PGE2 synthesis by SNP. 8-bromo guanosine 3':5'-cyclic monophosphate (8-bromo cGMP), a cGMP analogue, (10(-6), 10(-5), 10(-4), 10(-3) M) did not affect PGE2 synthesis. These findings suggest that NO increased PGE2 and PGI2 synthesis via a cGMP-independent pathway in cultured rabbit gastric cells.
Uno
H
H
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Arakawa
T
T
Fukuda
T
T
Yu
H
H
Fujiwara
Y
Y
Higuchi
K
K
Inoue
M
M
Kobayashi
K
K
D000818:Animals; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D011464:Epoprostenol / Q000096:biosynthesis; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006454:Hemoglobins / Q000494:pharmacology; D008751:Methylene Blue / Q000494:pharmacology; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism* / Q000493:pharmacokinetics; D019001:Nitric Oxide Synthase / Q000187:drug effects / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D019335:Nitroarginine / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis*; D011817:Rabbits; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D004396:Coloring Agents; D004791:Enzyme Inhibitors; D006454:Hemoglobins; D009566:Nitrates; D009573:Nitrites; D011453:Prostaglandins; D013439:Sulfhydryl Reagents; D009599:Nitroprusside; D019335:Nitroarginine; C016276:8-bromocyclic GMP; D009569:Nitric Oxide; D011464:Epoprostenol; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP; D015232:Dinoprostone; D008751:Methylene Blue