HLA-G gene polymorphism segregation within CEPH reference families.
HLA-G, a nonclassical HLA class I antigen, presents tissue-restricted expression on human trophoblasts and may play an important role in immune tolerance of mother-versus-fetus. In this work we have demonstrated extensive HLA-G genomic polymorphism within three CEPH reference families, by PCR-SSCP analysis and direct sequencing. Among six unrelated parents we assigned eight HLA-G alleles, seven of which are new. We observed the segregation of HLA-G alleles of heterozygous parents among their offspring that matched the segregation of the HLA class I haplotypes. Only one of the mutations observed was found to be nonsynonymous indicating low polymorphism of the HLA-G molecule.
Kirszenbaum
M
M
CEA-DRM-Service de Recherches en Hémato-Immunologie, Hôpital Saint Louis, Centre Hayem, Paris, France.
Djoulah
S
S
Hors
J
J
Le Gall
I
I
de Oliveira
E B
EB
Prost
S
S
Dausset
J
J
Carosella
E D
ED
D000483:Alleles; D002460:Cell Line; D005805:Genes, MHC Class I; D005819:Genetic Markers / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008540:Meiosis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012015:Reference Standards
D005819:Genetic Markers; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I