Regulation of lipolysis by the sympathetic nervous system: a microdialysis study in normal and spinal cord-injured subjects.
To evaluate the regulation of lipolysis by the sympathetic nervous system, eight spinal cord-injured (SCI) subjects with a lesion above T5 resulting in a decentralization of the lower-body sympathetic nervous system and adrenal medulla (age, 36 +/- 2 years; weight, 82 +/- 5 kg; body fat mass, 26.8 +/- 3.0 kg; all mean +/- SE) and nine control subjects (age, 33 +/- 2; weight, 80 +/- 3; NS; body fat mass, 16.1 +/- 1.5 kg; P < .01) were investigated after fasting overnight. Each subject was studied with subcutaneous microdialysis and 133Xe-clearance adipose tissue blood flow (ATBF) in the umbilical and clavicular regions during postabsorptive rest and after sympathoexcitatory stimulation by means of mental stress and isometric handgrip exercise. SCI subjects had an increased body fat mass, hyperinsulinemia, and an elevated lipolytic rate at rest compared with control subjects. ATBF and lipolysis were activated to a normal extent following mental stress and isometric handgrip exercise in the umbilical region in control subjects. ATBF was increased in tissue above but not below the lesion level in SCI subjects following mental stress. Glycerol release was not different between groups in either tissue region despite significantly lower noradrenaline and adrenaline levels in SCI subjects. This finding argues against a significant adrenergic control of the lipolytic rate at rest. Furthermore, the small differences in stimulated glycerol release between groups, as well as the increased plasma glycerol levels in SCI subjects, cast doubt on the view that interruption of adrenergic activity below the lesion is the sole mechanism underlying the increased body fat mass in SCI subjects.
Karlsson
A K
AK
Department of Clinical Neuroscience (Spinal Unit and Department of Clinical Neurophysiology), Rigshospitalet, University of Copenhagen, Denmark.
Elam
M
M
Friberg
P
P
Biering-Sörensen
F
F
Sullivan
L
L
Lönnroth
P
P
D000328:Adult; D001794:Blood Pressure; D005260:Female; D005990:Glycerol / Q000378:metabolism; D006339:Heart Rate; D006801:Humans; D008066:Lipolysis; D008297:Male; D017551:Microdialysis; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000503:physiopathology / Q000523:psychology; D013315:Stress, Psychological / Q000378:metabolism / Q000503:physiopathology; D013564:Sympathetic Nervous System / Q000378:metabolism*
D005990:Glycerol