Selective production of transgenic mice using green fluorescent protein as a marker.
The low efficiency of transgenic animal production by microinjection has been a serious problem especially for the production of transgenic livestock. We developed a method to selectively produce transgenic mice using green fluorescent protein (GFP) as a marker. Using this method, we obtained eight fetuses and four live-born mice derived from 55 GFP-positive blastocysts. PCR analysis showed 11 out of 12 mice (fetuses and newborn mice) were transgenic. Southern blot analysis showed that 8 out of 12 were transgenic. GFP expression was also observed in bovine blastocysts, suggesting that this method should contribute to the efficient production of transgenic livestock.
Takada
T
T
Molecular, Cell Pharmacology, National Children's Medical Research Center, Tokyo, Japan. ttakada@nch.go.jp
Iida
K
K
Awaji
T
T
Itoh
K
K
Takahashi
R
R
Shibui
A
A
Yoshida
K
K
Sugano
S
S
Tsujimoto
G
G
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D002417:Cattle; D003587:Cytomegalovirus / Q000235:genetics; D004624:Embryo Transfer; D004742:Enhancer Elements, Genetic; D005260:Female; D005306:Fertilization; D005819:Genetic Markers; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011555:Pseudopregnancy; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013539:Simian virus 40 / Q000235:genetics
D005819:Genetic Markers; D008164:Luminescent Proteins; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins