title: An animal model for external otitis.
abstract: External otitis was produced in 12 Sprague-Dawley rats by mechanical stimulation through a plastic micropipette inserted into the right external auditory canal (EAC). The EAC was later evaluated regarding the color of the skin, swelling and the presence of fluid. Within 1 day all rats developed an external otitis that was characterized by a red, swollen ear canal containing an opalescent fluid. The tympanic membrane and middle ear cavity appeared to be normal. No healed EACs were seen within the initial 10 days of follow-up and 4 of 6 rats still exhibited external otitis at day 21. Light microscopy of biopsy specimens revealed pronounced edema of the dermis of the ear canal. Mast cells were more numerous in the early phase of the otitis present, although very few inflammatory cells were found in tissues despite the marked inflammatory reaction produced. Findings show that this animal model for external otitis can be used to investigate pathogenesis as well as to test various treatment strategies.
authors.affiliation: Department of Otorhinolaryngology, University Hospital of Umeå, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Emgård
authors.forename: S
authors.initials: S
authors.lastname: Hellström
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004424:Ear Canal / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D008297:Male; D010032:Otitis Externa / Q000209:etiology* / Q000473:pathology; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014432:Tympanic Membrane / Q000473:pathology