title: Failure to detect Borna disease virus infection in peripheral blood leukocytes from humans with psychiatric disorders.
abstract: The presence of antibodies reactive with Borna disease virus (BDV) in the sera of some patients with certain psychiatric illnesses has been taken as evidence that this veterinary neurotrophic virus may occasionally infect and cause psychiatric disorders in humans. In this paper, we report the results of our studies concerning the detection of BDV-specific RNA in blood cells from patients with psychiatric diseases. Contrary to the results obtained by others, we have found no evidence for the presence of BDV-RNA in such cells. Prior work with BDV sequences in the assay environment, together with the exquisite sensitivity of RT-PCR, may account for the sporadic appearance of false positive evidence that BDV-specific RNA is present in human blood cells.
authors.affiliation: Institut für Virologie, Universitat Giessen, Germany.
authors.forename: J A
authors.initials: JA
authors.lastname: Richt
authors.forename: R C
authors.initials: RC
authors.lastname: Alexander
authors.forename: S
authors.initials: S
authors.lastname: Herzog
authors.forename: D C
authors.initials: DC
authors.lastname: Hooper
authors.forename: R
authors.initials: R
authors.lastname: Kean
authors.forename: S
authors.initials: S
authors.lastname: Spitsin
authors.forename: K
authors.initials: K
authors.lastname: Bechter
authors.forename: R
authors.initials: R
authors.lastname: Schüttler
authors.forename: H
authors.initials: H
authors.lastname: Feldmann
authors.forename: A
authors.initials: A
authors.lastname: Heiske
authors.forename: Z F
authors.initials: ZF
authors.lastname: Fu
authors.forename: B
authors.initials: B
authors.lastname: Dietzschold
authors.forename: R
authors.initials: R
authors.lastname: Rott
authors.forename: H
authors.initials: H
authors.lastname: Koprowski
mesh_terms: D000328:Adult; D000818:Animals; D001890:Borna Disease / Q000097:blood* / Q000150:complications / Q000175:diagnosis; D001891:Borna disease virus / Q000302:isolation & purification*; D015331:Cohort Studies; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000821:virology; D008297:Male; D001523:Mental Disorders / Q000097:blood / Q000209:etiology / Q000821:virology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood; D011817:Rabbits; D012559:Schizophrenia / Q000097:blood / Q000209:etiology / Q000821:virology; D012680:Sensitivity and Specificity
chemical_list: D012367:RNA, Viral