title: CD11a/CD18 and CD11b/18 modulation by lipoteichoic acid, N-acetyl-muramyl-alpha-alanyl-D-isoglutamine, muramic acid and protein A from Staphylococcus aureus.
abstract: This study investigates the effect of some components of the Staphylococcus aureus cell wall [lipoteichoic acid (LTA), N-acetyl-muramyl-alanyl-D-isoglutamine (MD), muramic acid (MA) and protein A (PA)] in modulating expression of cell-surface adhesion molecules CD11a/CD18, CD11b/C18 on monocytes qualitatively and quantitatively. Monocytes incubated with bacterial components presented different CD11b/CD18 expressions which were dose-dependent in contrast to controls. The results obtained demonstrated that lymphocytes incubated with bacterial components also increased the expression of CD11a/CD18. The modifications in activation of CD11a/CD18 and CD11b/CD18 expression are probably correlated with modifications of membrane fluidity measured as polarisation fluorescence (P).
authors.affiliation: Istituto di Microbiologia, Seconda Università degli Studi di Napoli, Facoltà di Medicina e Chirurgia, Naples, Italy.
authors.forename: C R
authors.initials: CR
authors.lastname: Carratelli
authors.forename: I
authors.initials: I
authors.lastname: Nuzzo
authors.forename: C
authors.initials: C
authors.lastname: Bentivoglio
authors.forename: M
authors.initials: M
authors.lastname: Galdiero
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D018821:CD18 Antigens / Q000096:biosynthesis; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002449:Cell Aggregation; D002462:Cell Membrane / Q000502:physiology; D002473:Cell Wall / Q000737:chemistry / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D008560:Membrane Fluidity; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D009112:Muramic Acids / Q000276:immunology / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013205:Staphylococcal Protein A / Q000276:immunology / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000276:immunology*; D013682:Teichoic Acids / Q000276:immunology / Q000494:pharmacology
chemical_list: D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D008070:Lipopolysaccharides; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D009112:Muramic Acids; D013205:Staphylococcal Protein A; D013682:Teichoic Acids; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C009900:lipoteichoic acid