title: Fractionation and characterization of boar seminal plasma spermadhesion PSP-II glycoforms reveal the presence of uncommon N-acetylgalactosamine-containing N-linked oligosaccharides.
abstract: Lectin mapping, carbohydrate analysis and electrospray mass spectrometry of boar seminal plasma PSP-II glycoforms show that its single N-glycosylation site displays a repertoire of carbohydrate structures consisting of the basic pentasaccharide core Man alpha 1-6[Man alpha 1-3]Man beta1-4GlcNAc beta1-4GlcNAc with a fucosyl residue alpha1-6-linked to the innermost N-acetylglucosamine residue. Other glycoforms display fucosylated hybrid-type or monoantennary complex-type chains, some of which contain alpha2-6-linked sialic acid. N-acetylgalactosamine, possibly in Gal beta1-3GalNAc sequence, is present in most of the PSP-II glycoforms.
authors.affiliation: Instituto de Quimíca-Física Rocasolano CSIC, Madrid, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Solis
authors.forename: J J
authors.initials: JJ
authors.lastname: Calvete
authors.forename: L
authors.initials: L
authors.lastname: Sanz
authors.forename: C
authors.initials: C
authors.lastname: Hettel
authors.forename: M
authors.initials: M
authors.lastname: Raida
authors.forename: T
authors.initials: T
authors.lastname: Diaz-Mauriño
authors.forename: E
authors.initials: E
authors.lastname: Töpfer-Petersen
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D012661:Semen / Q000737:chemistry*; D029607:Seminal Vesicle Secretory Proteins; D017421:Sequence Analysis; D013552:Swine
chemical_list: D006023:Glycoproteins; D037102:Lectins; D009005:Monosaccharides; D009844:Oligosaccharides; D029607:Seminal Vesicle Secretory Proteins; C074521:seminal vesicle secretory protein II, porcine; D000117:Acetylglucosamine