title: Role of the charged tail in localization of a surface protein antigen of Streptococcus mutans.
abstract: To make clear the role of the C terminus of a surface protein antigen (PAc) of Streptococcus mutans, stepwise truncations beginning at the C terminus of PAc were performed by utilizing site-directed mutagenesis. A remarkable increase in the amount of cell-free PAc was observed upon deletion of four or more amino acid residues at the C terminus. On the other hand, the amount of cell surface PAc gradually decreased when increasing numbers (four or more) of amino acid residues were deleted at the C terminus, and deletion of six amino acids involving both the total charged tail and Leu, an amino acid residue immediately upstream of the charged tail, resulted in a drastic reduction in the amount of cell surface PAc. These results indicate that the cytoplasmic charged tail and Leu residue are required for cell surface localization of PAc in S. mutans.
authors.affiliation: Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Higashi-ku, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: H O
authors.initials: HO
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: T
authors.initials: T
authors.lastname: Koga
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins; D015153:Blotting, Western; D008562:Membrane Glycoproteins; D016297:Mutagenesis, Site-Directed; D013291:Streptococcus / Q000235:genetics* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; C055764:S-layer proteins