title: Development of a simple restriction fragment length polymorphism (RFLP) based assay for HCV genotyping and comparative analysis with genotyping and serotyping tests.
abstract: The distribution between the different hepatitis C virus genotypes and subtypes has potentially important clinical and epidemiological implications. With this view a simple restriction fragment length polymorphism (RFLP) based assay was developed to identify the three major genotypes, 1, 2 and 3 and distinguish subtype 1a from 1b. This RFLP test uses a combination of three restriction endonuclease digestions, BstN1, BstU1 and Sau3a, respectively. Comparison with a 5'UTR based assay (LiPA), showed a 98% concordance with the RFLP based assay. In addition, good concordance is shown between these data and those obtained with a serological assay based on NS4 peptides.
authors.affiliation: Hybridotest, CBMS, Institut Pasteur, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Thiers
authors.forename: F
authors.initials: F
authors.lastname: Jaffredo
authors.forename: R
authors.initials: R
authors.lastname: Tuveri
authors.forename: N
authors.initials: N
authors.lastname: Chodan
authors.forename: C
authors.initials: C
authors.lastname: Bréchot
mesh_terms: D000918:Antibody Specificity; D015342:DNA Probes / Q000032:analysis / Q000235:genetics; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping / Q000145:classification*
chemical_list: D015342:DNA Probes