Purification and characterisation of the soluble glutathione S-transferase isoenzymes in rat kidney derived NRK cells.
Glutathione S-transferase (GST) enzymes are toxicologically important from many points of view. Rat kidney derived established NRK cells were mass cultured for the isolation of GST isoenzymes. These were enriched by affinity chromatography and separated by chromatofocusing and HPLC. Exactly the same major GST subunits were found in NRK cells as in the rat kidney. Strong evidence was also found for the presence of an aberrant form of GST 7-7, as was described in rat kidney. A very good correlation between the NRK GST and rat kidney, and especially cis-platinum treated kidney, was found. It is concluded that NRK cells can be considered as a valuable alternative tool for in vitro research of rat kidney phenomena, especially when toxicological interactions are investigated.
Dierickx
P J
PJ
Instituut voor Hygiëne en Epidemiologie, Afdeling Toxikologie, Brussel, Belgium.
Noble
E
E
D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism*; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D051381:Rats; D012995:Solubility
D004791:Enzyme Inhibitors; D007527:Isoenzymes; D005982:Glutathione Transferase