title: Which compartments are involved in Philadelphia-chromosome positive chronic myeloid leukaemia? An answer at the single cell level by combining May-Grünwald-Giemsa staining and fluorescence in situ hybridization techniques.
abstract: Chronic myeloid leukaemia (CML) is believed to represent a stem cell disorder involving all three cell lineages. The typical chromosomal aberration, the Philadelphia chromosome, is the translocation (9;22)(q34;q11). Several studies with cytogenetics, fluorescence in situ hybridization (FISH), or polymerase chain reaction have investigated the presence of the t(9;22) in different cell compartments. However, questions still remain. In six cases of CML we combined the standard May-Grünwald-Giemsa staining with FISH at the single-cell level and were able to demonstrate that not only all maturation stages of granulopoiesis, erythropoiesis, and megakaryocytes, but also plasma cells, eosinophils, basophils and monocytes carried the Philadelphia chromosome in 53-98% of samples. Using immunological identification of single cells we were able to demonstrate that the t(9;22) is detectable in 34% of CD3-positive T lymphocytes, in 32% of CD19-positive B lymphocytes, and in 82% of CD34-positive precursor cells. The results give new insight into the biology of CML and may have implications for future therapeutic strategies.
authors.affiliation: Department of Haematology and Oncology, University of Göttingen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Haferlach
authors.forename: M
authors.initials: M
authors.lastname: Winkemann
authors.forename: C
authors.initials: C
authors.lastname: Nickenig
authors.forename: M
authors.initials: M
authors.lastname: Meeder
authors.forename: L
authors.initials: L
authors.lastname: Ramm-Petersen
authors.forename: R
authors.initials: R
authors.lastname: Schoch
authors.forename: M
authors.initials: M
authors.lastname: Nickelsen
authors.forename: K
authors.initials: K
authors.lastname: Weber-Matthiesen
authors.forename: B
authors.initials: B
authors.lastname: Schlegelberger
authors.forename: C
authors.initials: C
authors.lastname: Schoch
authors.forename: W
authors.initials: W
authors.lastname: Gassmann
authors.forename: H
authors.initials: H
authors.lastname: Loffler
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001399:Azure Stains; D001402:B-Lymphocytes / Q000473:pathology; D019070:Cell Lineage; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000473:pathology; D014178:Translocation, Genetic
chemical_list: D001399:Azure Stains