GM-CSF downregulates expression of tryptase, Fc epsilon RI and histamine in HMC-1 mast cells.
Fibroblast-derived growth factors like SCF are able to upregulate the expression of mast cell characteristics in human multilineage hematopoietic progenitor cells. Other factors, like GM-CSF, have been reported to inhibit this process, probably by the competitive recruitment of cells not belonging to the mast cell lineage. In this study, we investigated the influence of GM-CSF on immature mast cells of the HMC-1 cell line which already show low-level expression of mast cell tryptase, histamine and Fc epsilonRI alpha. Culture of HMC-1 cells with mast-cell-conditioning medium, containing fibroblast supernatants, upregulated tryptase activity, histamine contents and expression of Fc epsilonRI alpha. However, addition of GM-CSF (10 ng/ml) markedly downregulated these mast cell markers, without affecting proliferation and viability of cells. Thus, GM-CSF may provide an inhibitory signal during mast cell differentiation and probably even downregulates mast cell characteristics in more differentiated cells.
Welker
P
P
Department of Dermatology, Virchow Clinics, Humboldt University, Berlin, Germany.
Grabbe
J
J
Zuberbier
T
T
Henz
B M
BM
D002478:Cells, Cultured; D053818:Chymases; D015536:Down-Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006632:Histamine / Q000032:analysis*; D006801:Humans; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D017455:Receptors, IgE / Q000032:analysis*; D012697:Serine Endopeptidases / Q000378:metabolism*; D053802:Tryptases
D017455:Receptors, IgE; D006632:Histamine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases