title: Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation.
abstract: We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.
authors.affiliation: Department of Oral Pathology, School of Dentistry, Showa University, Shinagawa-ku, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kinto
authors.forename: M
authors.initials: M
authors.lastname: Iwamoto
authors.forename: M
authors.initials: M
authors.lastname: Enomoto-Iwamoto
authors.forename: S
authors.initials: S
authors.lastname: Noji
authors.forename: H
authors.initials: H
authors.lastname: Ohuchi
authors.forename: H
authors.initials: H
authors.lastname: Yoshioka
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: Y
authors.initials: Y
authors.lastname: Wada
authors.forename: G
authors.initials: G
authors.lastname: Yuhao
authors.forename: H E
authors.initials: HE
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Yoshiki
authors.forename: A
authors.initials: A
authors.lastname: Yamaguchi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001846:Bone Development; D002454:Cell Differentiation; D002460:Cell Line; D002642:Chick Embryo; D018920:Coculture Techniques; D004627:Embryonic Induction; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D053823:Hedgehog Proteins; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D010006:Osteoblasts / Q000166:cytology* / Q000637:transplantation; D010446:Peptide Fragments / Q000096:biosynthesis; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D013997:Time Factors; D015534:Trans-Activators; D014162:Transfection
chemical_list: D053823:Hedgehog Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D015534:Trans-Activators