Mammalian cell binding and transfection mediated by surface-modified bacteriophage lambda.
Bacteriophage lambda virions whose tail tube major subunit (V) proteins are modified with a cyclizable Arg-Gly-Asp (RGD) peptide are able to promote the binding of certain mammalian cells to a solid surface. This effect was shown to be specific by peptide competition experiments, and control phage lacking the RGD peptide showed no significant cellular interaction. RGD-modified but not control phage bearing a reporter gene could transfect COS cells at a significant frequency. Phage-mediated transfection therefore benefits when the efficiency of only one step in the multi-stage uptake process is improved.
Dunn
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Queensland Cancer Fund Research Unit, Department of Pathology, Medical School, University of Queensland Brisbane, Australia.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics* / Q000378:metabolism; D016466:CHO Cells; D019556:COS Cells; D006224:Cricetinae; D017930:Genes, Reporter; D005822:Genetic Vectors; D019012:Integrin beta1 / Q000378:metabolism; D007763:Lac Operon; D008561:Membrane Fusion; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D019012:Integrin beta1; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid