Presence of c-Myc protein in murine myeloid leukaemia cells during growth and after irradiation.
Myeloid leukaemia (ML) is strain specific for RFM mice, which were used in these experiments. We investigated the presence of c-myc protein during the growth of ML and after irradiation of leukaemic cells. Leukaemic spleen cells were investigated 9 (nonterminal phase NTP) or 12 days (terminal phase TP) after the injection of ML cells. Leukaemic cells of NTP were irradiated with X-rays or UV-light. c-Myc protein was detected by immunocytochemical method. c-Myc protein was expressed in 74.98% of spleen cells of healthy RFM mice. In the early period of leukaemia growth (NTP) only 14.33% of c-myc positive cells were found, as opposed to the terminal phase (TP) of leukaemia when 89.7% of c-myc positive cells were detected. These results indicated the connection of growth of ML and the presence of c-myc protein. If the spleen cells of NTP of leukaemia were irradiated by X-rays or UV-light, the number of cells which expressed c-myc protein was extremely increased.
Popović-Hadzija
M
M
Division of Biology and Medicine, Ruder Bosković Institute, Zagreb, Croatia.
Poljak-Blazi
M
M
Pavelić
K
K
D000818:Animals; D007150:Immunohistochemistry; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D051379:Mice; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*
D016271:Proto-Oncogene Proteins c-myc