Tumor cell cytolysis mediated by valorphin, an opioid-like fragment of hemoglobin beta-chain.
Valorphin, an endogenous opioid-like hemoglobin fragment, is cytotoxic for L929 and K562 tumor cells in 10(-7)-10(-13) M concentration range. Because cytolytic effects induced by valorphin in K562 cells are inhibited by naloxone, opioid receptors should be involved in induction of valorphin-mediated tumor cell death. Three distinct cytolytic processes, differing in the onset time and the development time, take place with K562 cells within 10-18 h of incubation with valorphin. All three processes are not associated with apoptotic mechanism of cell death.
Blishchenko EYu
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Moscow, Russia.
Mernenko
O A
OA
Mirkina
I I
II
Satpaev
D K
DK
Ivanov
V S
VS
Tchikin
L D
LD
Ostrovsky
A G
AG
Karelin
A A
AA
Ivanov
V T
VT
D000218:Adamantane / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D016923:Cell Death / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D004744:Enkephalin, Methionine / Q000494:pharmacology; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D011957:Receptors, Opioid / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000701:Analgesics, Opioid; D006454:Hemoglobins; D011957:Receptors, Opioid; D009270:Naloxone; D004744:Enkephalin, Methionine; C045483:valorphin; D000218:Adamantane