Separation and quantitation of glycolipids as penetration modifiers in human skin using high-performance liquid chromatography-mass spectrometry with electrospray ionization.
An high-performance liquid chromatography-mass spectrometry method is presented for the measurement of glycolipids used as modulators of the penetration of drugs into human skin. In methanol extracts from different skin layers a detection limit of 100-400 pg/ml could be achieved. A routine analytical procedure could be set up with good quantitation reliability (relative standard deviation 6.6%).
Wolf
R
R
Martin-Luther-University, Department of Pharmacy, Halle (Saale), Germany.
Raith
K
K
Neubert
R
R
D000277:Adjuvants, Pharmaceutic / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006017:Glycolipids / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012867:Skin / Q000737:chemistry* / Q000187:drug effects; D012869:Skin Absorption / Q000187:drug effects*
D000277:Adjuvants, Pharmaceutic; D006017:Glycolipids; D007202:Indicators and Reagents