title: Incorporation of an active site inhibitor in factor VIIa alters the affinity for tissue factor.
abstract: Recent studies showed that the administration of active site-inhibited factor VIIa blocked factor VIIa/tissue factor-induced fibrin and thrombus formation in ex vivo and in vivo model systems. These studies suggest that inactivated factor VIIa competes efficiently with plasma factor VII(a) for a limited number of tissue factor sites. In the present study, we compared the interactions of factor VIIa and active site-inhibited factor VIIa with tissue factor. Competition studies of factor VIIa and active site-inhibited factor VIIa in a factor X activation assay showed that the affinity of the latter for relipidated tissue factor was 5-fold higher than that of factor VIIa. Radioligand binding studies with a human bladder carcinoma cell line (J82) and surface plasmon resonance studies using soluble tissue factor demonstrated a faster association and a slower dissociation for the active site-inhibited factor VIIa. Studies of equilibrium binding to cell surface tissue factor showed that the affinity of active site-inhibited VIIa was 5-fold higher than that of factor VIIa to non-functional tissue factor sites, whereas both inactivated factor VIIa and factor VIIa bound to functional tissue factor sites with the same high affinity. Comparison of the CD spectra of factor VIIa and active site-inactivated factor VIIa revealed structural differences in the protease domain. The potential physiological implications of these findings are discussed.
authors.affiliation: Vessel Wall Biology, Health Care Discovery, Novo Nordisk A/S, Niels Steensens Vej 1, DK-2820 Gentofte, Denmark. bbsn@novo.dk
authors.forename: B B
authors.initials: BB
authors.lastname: Sorensen
authors.forename: E
authors.initials: E
authors.lastname: Persson
authors.forename: P O
authors.initials: PO
authors.lastname: Freskgârd
authors.forename: M
authors.initials: M
authors.lastname: Kjalke
authors.forename: M
authors.initials: M
authors.lastname: Ezban
authors.forename: T
authors.initials: T
authors.lastname: Williams
authors.forename: L V
authors.initials: LV
authors.lastname: Rao
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002942:Circular Dichroism; D015942:Factor VIIa / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000378:metabolism; D013925:Thromboplastin / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D011994:Recombinant Proteins; C027587:phenylalanyl-phenylalanyl-arginine chloromethyl ketone; D005337:Fibrin; D013925:Thromboplastin; D015942:Factor VIIa