title: Identification of vitellogenin receptors in the ovary of a teleost fish, the Mediterranean sea bass (Dicentrarchus labrax).
abstract: The characterization of vitellogenin (VTG) receptors in ovarian membranes from vitellogenic female sea bass (Dicentrarchus labrax) is described. Incubation of membrane proteins with radiolabeled VTG (125I-VTG) after SDS-electrophoresis showed specific binding of 125I-VTG to a protein band of 100 kDa. Filter binding assays showed that binding of 125I-VTG to membrane receptors was saturable with increasing amounts of 125I-VTG. Scatchard analysis of the saturation data revealed a single class of binding sites with an apparent KD of 1.04 x 10(-8) M. The specificity of the VTG receptors was tested in competition assays; binding of 125I-VTG to ovarian membranes was completely abolished with an excess of purified sea bass VTG (cold VTG, VTG degree) or plasma from estradiol (E2)-treated fish, while the addition of control male plasma (without VTG) caused negligible effect.
authors.affiliation: Instituto de Acuicultura de Torre la Sal (CSIC), Castellón, Spain.
authors.forename: E L
authors.initials: EL
authors.lastname: Mañanós
authors.forename: J
authors.initials: J
authors.lastname: Núñez Rodríguez
authors.forename: F
authors.initials: F
authors.lastname: Le Menn
authors.forename: S
authors.initials: S
authors.lastname: Sanuy
authors.forename: M
authors.initials: M
authors.lastname: Carrillo
mesh_terms: D000818:Animals; D001345:Autoradiography; D001492:Bass; D001667:Binding, Competitive; D002462:Cell Membrane / Q000737:chemistry; D004527:Egg Proteins; D005260:Female; D007457:Iodine Radioisotopes; D008297:Male; D010053:Ovary / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism; D014819:Vitellogenins / Q000378:metabolism*
chemical_list: D004527:Egg Proteins; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D014819:Vitellogenins; C030474:vitellogenin receptor