title: Human red blood cell membrane potential and fluidity in glucose solutions.
abstract: This study aimed to investigate the membrane potential and fluidity changes of human red blood cells subjected to isotonic glucose solutions. For the control erythrocytes a membrane potential value of -10.1 +/- 1.8 mV was obtained, whereas erythrocyte membranes in diabetic cells were hyperpolarized, with a potential of -13.9 +/- 2.3 mV. Incubation of both types of red blood cells with increasing glucose concentrations resulted in a substantial hyperpolarization of the cell membranes. Glucose solutions in water had a much stronger effect on membrane potential than glucose dissolved in phosphate-buffered saline. Red blood cell membrane fluidity measurements using the fluorescent label TMADPH did not reveal any significant changes upon incubation with glucose.
authors.affiliation: Institute of Biochemistry, Academy of Sciences of Belarus, Grodno.
authors.forename: I B
authors.initials: IB
authors.lastname: Zavodnik
authors.forename: A
authors.initials: A
authors.lastname: Piasecka
authors.forename: K
authors.initials: K
authors.lastname: Szosland
authors.forename: M
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D052160:Benzothiazoles; D002232:Carbocyanines; D003920:Diabetes Mellitus / Q000097:blood; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000502:physiology*; D005456:Fluorescent Dyes; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007552:Isotonic Solutions; D008560:Membrane Fluidity / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D007552:Isotonic Solutions; D004161:Diphenylhexatriene; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; D005947:Glucose