title: Immunological characterization of a neuronal antibody (anti-Tr) associated with paraneoplastic cerebellar degeneration and Hodgkin's disease.
abstract: We studied an autoantibody (called anti-Tr), found in the serum and CSF of five patients with paraneoplastic cerebellar degeneration (PCD) and Hodgkin's disease (HD). Anti-Tr antibodies labelled the cytoplasm of Purkinje cells of human and rat cerebellum. The molecular layer of rat cerebellum showed a characteristic dotted pattern suggestive of immunoreactivity of dendritic spines of Purkinje cells. Patients with cerebellar disorders without HD (159) or HD without PCD (30) did not harbor anti-Tr antibodies. Immunoblots of human Purkinje cells or rat and mouse cerebellum were negative. Anti-Tr antibodies, as defined in this study, appear specific for HD-associated PCD. The immunohistochemical pattern described in the rat cerebellum coupled with the absence of reactivity in the immunoblot may be used to identify anti-Tr antibodies.
authors.affiliation: Service of Neurology, Hospital Clínic i Provincial, Barcelona, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Graus
authors.forename: J
authors.initials: J
authors.lastname: Dalmau
authors.forename: F
authors.initials: F
authors.lastname: Valldeoriola
authors.forename: I
authors.initials: I
authors.lastname: Ferrer
authors.forename: R
authors.initials: R
authors.lastname: Reñe
authors.forename: C
authors.initials: C
authors.lastname: Marin
authors.forename: C J
authors.initials: CJ
authors.lastname: Vecht
authors.forename: T
authors.initials: T
authors.lastname: Arbizu
authors.forename: C
authors.initials: C
authors.lastname: Targa
authors.forename: J W
authors.initials: JW
authors.lastname: Moll
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000134:cerebrospinal fluid / Q000276:immunology*; D002526:Cerebellar Diseases / Q000150:complications / Q000276:immunology*; D005260:Female; D006689:Hodgkin Disease / Q000134:cerebrospinal fluid / Q000150:complications / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009410:Nerve Degeneration; D009474:Neurons / Q000276:immunology*; D010257:Paraneoplastic Syndromes / Q000134:cerebrospinal fluid / Q000150:complications / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D000906:Antibodies