Clear detection and typing of herpes simplex virus types 1 and 2 by an indirect ELISA assay: comparison with three different combined methods--capture ELISA, restriction enzymes, and polymerase chain reaction.
The severity and recurrences of Herpes Simplex Virus (HSV) infection depend on the type of the infectious agent (HSV-1 or HSV-2), which induces the necessity of a nonambiguous detecting typing. The commonly used capture ELISA technique has to be often supported by DNA analysis to confirm the detection and the typing of HSV viruses in exposed patients. In this report, we describe a rapid and cheap indirect ELISA method using anti-HSV monospecific polyclonal antibodies prepared in the laboratory. The typing of the studied samples was clear, did not need series of dilution, and allowed the immediate classification of viruses without further control examination. We tested 51 specimens, which were typed 25 HSV-1 and 26 HSV-2 strains. The comparison with capture ELISA, restriction enzyme and polymerase chain reaction analysis definitely allowed our method to be assessed as a useful tool for a routine diagnostic.
Markoulatos
P
P
Virology Department, Hellenic Pasteur Institute, Athens, Greece.
Fountoucidou
P
P
Marinakis
G
G
Krikelis
V
V
Spyrou
N
N
Vamvakopoulos
N
N
Moncany
M L
ML
D000914:Antibodies, Viral / Q000032:analysis*; D017931:DNA Primers / Q000737:chemistry; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006558:Herpes Genitalis / Q000175:diagnosis*; D006561:Herpes Simplex / Q000175:diagnosis*; D018259:Herpesvirus 1, Human / Q000145:classification* / Q000235:genetics / Q000276:immunology; D018258:Herpesvirus 2, Human / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015183:Restriction Mapping / Q000379:methods*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic; D004262:DNA Restriction Enzymes