title: Evaluation of commercially available tests for Chlamydia nucleic acid detection in synovial fluid of patients.
abstract: Since the presence of Chlamydia nucleic acids has been shown in synovial fluid (SF) from some patients with Chlamydia reactive arthritis, we investigated whether commercially available tests, developed to detect Chlamydia nucleic acids in urogenital samples, could also be used for their detection in SF samples. We therefore tested SF samples, found positive with at least two different systems of DNA amplification in a previous study, with three commercially available kits. No positive results were obtained. It is concluded that the commercially available tests Gen-Probe PACE 2, Amplicor (developed by Roche Molecular Systems) and LCx (developed by Abbott Laboratories) do not have sufficient sensitivity to detect reliably Chlamydia RNA or DNA in SF.
authors.affiliation: Division of Rheumatology, University Hospital, Geneva, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Bas
authors.forename: B
authors.initials: B
authors.lastname: Ninet
authors.forename: O
authors.initials: O
authors.lastname: Delaspre
authors.forename: T L
authors.initials: TL
authors.lastname: Vischer
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis; D016918:Arthritis, Reactive / Q000175:diagnosis*; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006801:Humans; D012335:RNA, Ribosomal / Q000032:analysis*; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012680:Sensitivity and Specificity; D013582:Synovial Fluid / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D012335:RNA, Ribosomal; D011933:Reagent Kits, Diagnostic