Cloning of sucrase operon with mini-Mu and plasmid-mediated metabolism of sucrose.
The in vivo cloning system based on mini-Mu derivatives was used for cloning the sucrase operon. We constructed several recombinant plasmids pJT21, pMM2324, pMM2325 with a complete sucrase operon. Two strains of Escherichia coli containing this plasmid replicon were able to grow on different concentrations of sucrose. The stability of recombinant plasmids was determined after cultivation under nonselective conditions. The stability after 5-d cultivation was higher than 75%.
Grones
J
J
Department of Molecular Biology, Faculty of Science, Comenius University, Bratislava, Slovak Republic.
Macor
M
M
Bilská
V
V
D010583:Bacteriophage mu / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism* / Q000821:virology; D005798:Genes, Bacterial; D005822:Genetic Vectors; D009876:Operon; D010957:Plasmids; D012093:Replicon; D013393:Sucrase / Q000235:genetics* / Q000378:metabolism*
D013393:Sucrase