Screening of a large number of dyes for the separation of human immunoglobulin G2 from the other immunoglobulin G subclasses immunoglobulin G2 enrichment on immobilized Procion Yellow HE-4R.
Human immunoglobulins (IgG) are produced on a multi-ton scale for therapeutic applications. There is presently no available method to manufacture IgG preparations enriched with immunoglobulins from the IgG2 subclass although they might be useful for therapeutic purposes. By frontal chromatography, we have screened 69 immobilized dyes, among which, six display a different affinity for IgG2 and other subclasses. One (Procion Yellow HE-4R) was studied further. The screening of various mobile phase conditions allowed us to devise a procedure to prepare IgG2 enriched IgG solutions: The cumulative yield for IgG2 was 43% and IgG2/total IgG ratio in the final product was 67%.
Hasnaoui
M H
MH
INSERM U 76, Paris, France.
Debbia
M
M
Cochet
S
S
Cartron
J P
JP
Lambin
P
P
Bertrand
O
O
D000345:Affinity Labels; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D004396:Coloring Agents / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D014227:Triazines / Q000737:chemistry*
D000345:Affinity Labels; D004396:Coloring Agents; D007074:Immunoglobulin G; D014227:Triazines; C009794:procion yellow