title: Quantification of phosphorus metabolites in human calf muscle and soft-tissue tumours from localized MR spectra acquired using surface coils.
abstract: Metabolite concentrations determined from MR spectra provide more specific information than peak area ratios. This paper presents a method of quantification that allows metabolite concentrations to be determined from in vivo 31P MR spectra acquired using a surface coil and ISIS localization. Corrections for the effects of B1 field inhomogeneity produced by surface coils are based on a measured and calibrated spatial sensitivity field map for the coil. Account is taken of imperfections in pulse performance, coil loading effects and relaxation effects, the latter making use of published metabolite relaxation times. The technique is demonstrated on model solutions. The concentrations of the main 31P metabolites in normal human calf muscle measured using this method are [PCr] = 26.9 +/- 4.1 mM; [Pi] = 3.6 +/- 1.2 mM; [NTP] = 6.8 +/- 1.8 mM. Quantification of spectra acquired from soft-tissue tumours in patients both pre- and post-treatment showed that changes in metabolite concentrations are more sensitive to metabolic changes than changes in peak area ratios.
authors.affiliation: CRC Clinical Magnetic Resonance Research Group, Institute of Cancer Research and Royal Marsden NHS Trust, Sutton, Surrey, UK.
authors.forename: V L
authors.initials: VL
authors.lastname: Doyle
authors.forename: G S
authors.initials: GS
authors.lastname: Payne
authors.forename: D J
authors.initials: DJ
authors.lastname: Collins
authors.forename: M W
authors.initials: MW
authors.lastname: Verrill
authors.forename: M O
authors.initials: MO
authors.lastname: Leach
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D005682:Gadolinium; D019786:Gadolinium DTPA; D006801:Humans; D007866:Leg; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods; D019042:Muscle Neoplasms / Q000378:metabolism* / Q000556:secondary; D018482:Muscle, Skeletal / Q000378:metabolism*; D018358:Neuroendocrine Tumors / Q000378:metabolism / Q000556:secondary; D009942:Organometallic Compounds; D004369:Pentetic Acid / Q000031:analogs & derivatives; D010710:Phosphates / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D010758:Phosphorus; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012983:Soft Tissue Neoplasms / Q000378:metabolism*
chemical_list: D009942:Organometallic Compounds; D010710:Phosphates; D010725:Phosphocreatine; D010758:Phosphorus; D004369:Pentetic Acid; D000255:Adenosine Triphosphate; D005682:Gadolinium; D019786:Gadolinium DTPA