The endoplasmic reticulum-resident stress protein gp96 binds peptides translocated by TAP.
The endoplasmic reticulum (ER)-resident stress protein gp96 induces a major histocompatibility complex class I-restricted cytotoxic T lymphocyte (CTL) response against antigens present in the cells from which it has been prepared. In this study, photoreactive peptides were translocated into the ER by the transporter associated with antigen processing (TAP). These peptides can be cross-linked specifically to gp96. Thus, we provide the first evidence that gp96 binds TAP-translocated peptides which have been implicated in the induction of specific CTL responses after immunization with gp96 (Srivastava, P. K. et al., Immunogenetics 1994. 39: 93).
Lammert
E
E
Department of Immunology, Institute for Cell Biology, University of Tübingen, Germany.
Arnold
D
D
Nijenhuis
M
M
Momburg
F
F
Hämmerling
G J
GJ
Brunner
J
J
Stevanović
S
S
Rammensee
H G
HG
Schild
H
H
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism / Q000502:physiology*; D000345:Affinity Labels; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D001692:Biological Transport; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D014162:Transfection; D014443:Tyrosine / Q000378:metabolism
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D000345:Affinity Labels; D000951:Antigens, Neoplasm; D006360:Heat-Shock Proteins; D010455:Peptides; C073119:TAP1 protein, human; C486172:Tap1 protein, rat; C489514:Tap2 protein, rat; C052646:sarcoma glycoprotein gp96 rejection antigens; C071692:TAP2 protein, human; D014443:Tyrosine