[The isolation of Arcanobacterium haemolyticum from the pharyngeal exudate of children].
A. haemolyticum is involved in acute pharingotonsillitis in present, reaching a maximal incidence between 10 and 30 years of age. The authors examined 7402 throats swabs obtained from children between March 1995 and February 1996. The swabs were plated on a selective culture medium: 5% sheep blood agar added with 3.5% NaCl; the plates were incubated for 48 h in aerobic conditions. The colonies of A. haemolyticum have been suspected on the basis of a) the growing time interval (48 h); reduced size (diameter less than 1 mm); c) the presence of a narrow zone of incomplete beta-haemolysis and d) the typical pitting of the culture medium beneath the colony. The definitive identification was realised on the basis of the microscopical appearance (diphtheroid gram-positive bacilli), negative catalase test and inverse CAMP test (narrowing of the haemolytic zone produced by staphylococcal beta-lysine). The frequency of A. haemolyticum in the throat swabs investigated was 0.07% (5 cases), comparing with S. pyogenes which was found in 8.21% of cases.
Coman
G
G
Universitatea de Medicină şi Farmacie Iaşi.
Pânzaru
C
C
Dahorea
C
C
D000191:Actinomycetaceae / Q000187:drug effects / Q000302:isolation & purification*; D000193:Actinomycetales Infections / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D010612:Pharyngitis / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000302:isolation & purification
D000900:Anti-Bacterial Agents