Cloning sequence-specific DNA-binding factors from cDNA expression libraries using oligonucleotide binding site probes.
Cowell
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Department of Biochemistry and Genetics, Medical School, University of Newcastle, Newcastle upon Tyne, UK.
D000818:Animals; D010582:Bacteriophage lambda; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000378:metabolism; D004926:Escherichia coli; D015723:Gene Library; D007202:Indicators and Reagents; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism; D013379:Substrate Specificity
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins