A novel DR beta 1*03 allele identified by polymerase chain reaction-restriction fragment length polymorphism.
This report describes a new MHC class II allele, HLA-DR beta 1*0306, discovered in a 31-year-old Norwegian male. The allele typed serologically as DRw52 (DR3) and amplified in PCR using DR52-associated group primers. This product could not be identified using established restriction digests, however. Use of Asp 700, Msp I, Hha I, Bse RI, Mnl I, Hph I, and Bsrb I gave banding patterns expected for a DR beta 1*03011 allele, but Rsa I had an additional site at codon 47. Sequencing showed a single base change at this position, with the substitution of tyrosine for phenylanine in this new allele. The biologic impact of this substitution remains to be determined.
Fenske
B
B
Immunology Laboratory, Department of Pathology and Laboratory Medicine, Vancouver Hospital and Health Sciences Centre, Vancouver, Canada.
Lemieux
J
J
Hoar
D
D
Fitzpatrick
J
J
Keown
P
P
D000328:Adult; D000483:Alleles; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains