Osteopontin gene expression and protein synthesis in cultured rat mesangial cells.
It is controversial whether osteopontin (OP) is expressed in glomeruli and involved in glomerular diseases. We examined whether the OP expression is present at gene and protein levels in cultured rat mesangial cells (MCs). Northern blotting revealed a 1.7 kb OP-mRNA expression in MCs. Fetal calf serum (FCS) and TNF-alpha increased OP gene expression in serum-starved MCs by 2.7- and 1.8-fold over 24- and 12-hour periods, respectively. PDGF, IL-1beta, and TGF-beta had little effect on OP gene expression. Western blotting detected the OP protein expression (69 kDa). FCS and TNF-alpha increased OP protein expression in serum-starved MCs over 48- and 24-hour periods, respectively. The present study clearly demonstrated the expression of OP gene and protein in cultured rat MCs. Increased OP production under serum or TNF-alpha stimulation suggests that intraglomerular OP may contribute to the development of glomerular diseases.
Nagasaki
T
T
Second Department of Biochemistry, Osaka City University Medical School, Abeno-ku, Osaka, Japan.
Ishimura
E
E
Shioi
A
A
Jono
S
S
Inaba
M
M
Nishizawa
Y
Y
Morii
H
H
Otani
S
S
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D053495:Osteopontin; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000235:genetics* / Q000378:metabolism
D016207:Cytokines; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin