title: Platelet function in septic multiple organ dysfunction syndrome.
abstract: OBJECTIVE
  Altered platelet function plays a role in the pathophysiology of multiple organ failure in sepsis. The purpose of the present study was to evaluate various aspects of platelet adhesive function in septic patients and its putative relevance for prognosis.
  
  
  METHODS
  Prospective clinical study.
  
  
  METHODS
  Intensive Care Unit of the University Hospital.
  
  
  METHODS
  A total of 41 patients admitted to the medical Intensive Care Unit were studied. On the day of admission, patients were evaluated by intensive care scoring systems (Elebute, APACHE II) to assess the severity of sepsis and multiple organ dysfunction syndrome (MODS), and platelet function tests were performed. All patients were observed for 28 days to assess their clinical outcomes. Eleven patients revealed septicemia without MODS (Elebute > or = 12, APACHE II < 20) and 20 septic patients suffered from MODS (Elebute > or = 12, APACHE II > or = 20). Ten non-septic patients without MODS served as a control group (Elebute < 12, APACHE II < 20). Flow cytometric determination of the activated fibrinogen (fg) receptor GPIIb-IIIa and as well as thrombospondin (TSP) on platelets and platelet-neutrophil adhesion (CD 41 immunofluorescence) ex vivo was performed using monoclonal antibodies. The effect of plasma obtained from patients on normal platelet aggregation in vitro, and adhesion to cultured endothelial cells was evaluated.
  
  
  RESULTS
  The surface expression of TSP on platelets was increased in septic patients with MODS compared to controls (p < 0.03). Platelet-neutrophil adhesion was not significantly altered in septicemia (p < 0.09) but decreased significantly in the presence of MODS (p < 0.05) when compared to controls. Logistic regression analysis showed that platelet-neutrophil adhesion was an independent predictor for poor clinical outcome (p < 0.01). Plasma from septic patients sensitized normal platelets to hyperaggregate and to adhere to cultured endothelium (p < 0.01).
  
  
  CONCLUSIONS
  In septic patients platelets become activated and are hyperadhesive to other vascular cells including neutrophils and endothelium. This may induce sequestration of platelets and microcirculatory arrest, thus the development of MODS.
authors.affiliation: Medizinische Klinik, Technischen Universität München, Klinikum Rechts der Isar, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Gawaz
authors.forename: T
authors.initials: T
authors.lastname: Dickfeld
authors.forename: C
authors.initials: C
authors.lastname: Bogner
authors.forename: S
authors.initials: S
authors.lastname: Fateh-Moghadam
authors.forename: F J
authors.initials: FJ
authors.lastname: Neumann
mesh_terms: D018806:APACHE; D000328:Adult; D000368:Aged; D015415:Biomarkers; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000502:physiology; D003430:Cross-Sectional Studies; D004730:Endothelium, Vascular / Q000502:physiology; D005260:Female; D006801:Humans; D007362:Intensive Care Units / Q000706:statistics & numerical data; D016015:Logistic Models; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000097:blood / Q000503:physiopathology*; D009504:Neutrophils / Q000502:physiology; D015539:Platelet Activation / Q000502:physiology*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000502:physiology; D011446:Prospective Studies; D017741:Survivors / Q000706:statistics & numerical data; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000503:physiopathology*; D019699:Thrombospondins
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019699:Thrombospondins