title: Short- and long-term effects of low-dose prenatal X-irradiation in mouse cerebral cortex, with special reference to neuronal migration.
abstract: To elucidate the short- and long-term effects of ionizing radiation on cell migration in the developing cerebral cortex, we labeled proliferating cells on day 14 of gestation of mice with bromodeoxyuridine (BrdU) followed by a single exposure to 0.1-1 Gy of X-rays. The brains of embryos on day 17 and offspring at 2, 3 and 8 weeks after birth were processed for BrdU immunohistochemistry to trace the movements of BrdU-labeled cells. The location of BrdU-labeled neurons in the cerebral cortex was quantitatively analyzed between irradiated animals and non-irradiated controls. We have demonstrated that the initial migration of BrdU-labeled cells from the matrix cell zone towards the cortical plate during embryonic periods was decelerated when exposed to X-rays of 0.25, 0.5 and 1 Gy on embryonic day 14, and that aberrantly placed neurons in the cerebral neocortex were noted in younger animals that were irradiated prenatally, whereas such derangement was less pronounced in mature animals. These observations suggest that some modification process might have occurred during the postnatal period.
authors.affiliation: Department of Dynamic Pathology, Kyoto Prefectural University of Medicine, Japan. sfushiki@koto.kpu-m.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Fushiki
authors.forename: Y
authors.initials: Y
authors.lastname: Hyodo-Taguchi
authors.forename: C
authors.initials: C
authors.lastname: Kinoshita
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Hirobe
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001973:Bromodeoxyuridine; D002465:Cell Movement / Q000528:radiation effects*; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development* / Q000528:radiation effects*; D005260:Female; D005865:Gestational Age; D007150:Immunohistochemistry; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000528:radiation effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D013997:Time Factors
chemical_list: D000963:Antimetabolites; D001973:Bromodeoxyuridine