title: Structural characterization of lipid A obtained from Pantoea agglomerans lipopolysaccharide.
abstract: Lipopolysaccharide isolated from Pantoea agglomerans showed higher priming and triggering activities for macrophages in terms of tumor necrosis factor production than other lipopolysaccharides. To identify the difference in biological activities of lipopolysaccharide of Pantoea agglomerans from other lipopolysaccharides on the basis of structure, we determined the structure of the lipid A part, which is the biological center of lipopolysaccharides, by quantitative analysis, nuclear magnetic resonance spectroscopy and mass spectrometry. Lipopolysaccharide of Pantoea agglomerans is constructed with at least two kinds of lipid A of different levels of acylation. One is of the same type as that of Escherichia coli with hexa-acyl lipid A and the other is the Salmonella minnesota type with hepta-acyl lipid A.
authors.affiliation: Chiba Flour Milling Co., Japan.
authors.forename: D
authors.initials: D
authors.lastname: Tsukioka
authors.forename: T
authors.initials: T
authors.lastname: Nishizawa
authors.forename: T
authors.initials: T
authors.lastname: Miyase
authors.forename: K
authors.initials: K
authors.lastname: Achiwa
authors.forename: T
authors.initials: T
authors.lastname: Suda
authors.forename: G
authors.initials: G
authors.lastname: Soma
authors.forename: D
authors.initials: D
authors.lastname: Mizuno
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D004755:Enterobacteriaceae / Q000737:chemistry*; D008050:Lipid A / Q000032:analysis* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D008050:Lipid A