Characterization of a site-directed mutant of cytochrome b5 designed to alter axial imidazole ligand plane orientation.
Mutants of cytochrome b5 were designed to achieve reorientation of individual axial imidazole ligands. The orientation of the axial ligand planes is thought to modulate the reduction potential of bis(imidazole) axially ligated heme proteins. The A67V mutation achieved this goal through the substitution of a bulkier, hydrophobic ligand for a residue, in the sterically hindered hydrophobic heme binding pocket. Solution structures of mutant and wild-type proteins in the region of the mutation were calculated using restraints obtained from 1H and 15N 2D homonuclear and heteronuclear NMR spectra and 1H-15N 3D heteronuclear NMR spectra. More than 10 restraints per residue were used in the refinement of both structures. Average local rmsd for 20 refined structures was 0.30 A for the wild-type structure and 0.38 A for the A67V mutant. The transfer of amide proton resonance assignments from wild-type to the mutant protein was achieved through overlays of 15N-1H heteronuclear correlation spectra of the reduced proteins. Side chain assignments and sequential assignments were established using conventional assignment strategies. Calculation of the orientation of the components of the anisotropic paramagnetic susceptibility tensor, using methods similar to procedures applied to the wild-type protein, shows that the orientation of the in-plane components are identical in the wild-type and mutant proteins. However, the orientation of the z-component of the susceptibility tensor calculated for the mutant protein differs by 17 degrees for the A-form and by 11 degrees for the B-form from the orientation calculated for the wild-type protein. The rotation of the z-component of the susceptibility tensor (toward the delta meso proton) is in the same direction and is of the same magnitude as the rotation of the H63 imidazole ring induced by mutation.
Sarma
S
S
Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland at Baltimore 21201, USA.
Dangi
B
B
Yan
C
C
DiGate
R J
RJ
Banville
D L
DL
Guiles
R D
RD
D000577:Amides / Q000737:chemistry; D000818:Animals; D015786:Cytochromes b5 / Q000737:chemistry* / Q000235:genetics*; D006418:Heme / Q000737:chemistry / Q000235:genetics; D007093:Imidazoles / Q000737:chemistry*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D051381:Rats; D012996:Solutions; D013237:Stereoisomerism
D000577:Amides; D007093:Imidazoles; D008024:Ligands; D012996:Solutions; D006418:Heme; D015786:Cytochromes b5