title: Establishment of a new cell line from a patient with hairy cell leukemia-Japanese variant.
abstract: A cell line, JHC-2, was established from the peripheral blood of a patient with hairy cell leukemia (HCL)-Japanese variant. The JHC-2 cells have cytologic features similar to those of the original tumor cells. They displayed hairy cytoplasmic projections by phase contrast and scanning electron microscopy. The tartrate-resistant acid phosphatase reaction was weakly positive. The immunophenotype of the JHC-2 cells was CD5-, CD10-, CD11c+/-, CD19+, CD21+, CD23+, CD24-, CD25+/-, CD38- and FMC-7+. The expression of surface immunoglobulin (IgG, kappa) and the configuration of Ig gene rearrangements in the JHC-2 cells were identical to those in the original leukemic cells, and the JHC-2 cells displayed trisomy 9 on cytogenetic examination. Southern blot analysis for the Epstein-Barr virus (EBV) genome showed that the JHC-2 cells contained the EBV genome, although the freshly isolated leukemic cells did not. These results indicate that the JHC-2 cell line is an EBV spontaneously transformed B cell line originating from HCL cells.
authors.affiliation: Department of Hematology, Osaka University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shibayama
authors.forename: T
authors.initials: T
authors.lastname: Machii
authors.forename: Y
authors.initials: Y
authors.lastname: Tokumine
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimori
authors.forename: J
authors.initials: J
authors.lastname: Nojima
authors.forename: R
authors.initials: R
authors.lastname: Inoue
authors.forename: A
authors.initials: A
authors.lastname: Kanamaru
authors.forename: S
authors.initials: S
authors.lastname: Tagawa
authors.forename: T
authors.initials: T
authors.lastname: Kitani
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D015321:Gene Rearrangement; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics* / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D007564:Japan; D007943:Leukemia, Hairy Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007527:Isoenzymes; D015375:Receptors, Interleukin-2; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase