Adenovirus-receptor interaction with human lymphocytes.
Lymphocytes play a key role in cell-mediated immunity and are host cells for several viral and bacterial pathogens. Their importance in adenovirus (Ad) infections is not yet fully understood. The initial event, the attachment of Ad to lymphocytes and their subsets, was examined using flow cytometry. The study included analysis of stimulated T cells in binding assays with FITC-labeled Ad fiber. The results confirm that native peripheral lymphocytes express very small amounts of Ad receptors. Stimulation with PHA and interleukin 2 induced the expression. The presence of Ad DNA as a sign of internalization in stimulated cells was demonstrated using the polymerase chain reaction. The findings suggest that lymphocytes after stimulation can turn into target cells for Ad. This is particularly important if there are indications for persistence of Ad, and in the case of immunocompromised patients severe, life-threatening diseases can develop.
Mentel
R
R
Institut für Medizinische Mikrobiologie der Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Döpping
G
G
Wegner
U
U
Seidel
W
W
Liebermann
H
H
Döhner
L
L
D000260:Adenoviruses, Human / Q000276:immunology / Q000502:physiology*; D000956:Antigens, Viral / Q000502:physiology; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D036022:Capsid Proteins; D002478:Cells, Cultured; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000821:virology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011991:Receptors, Virus / Q000096:biosynthesis / Q000502:physiology*; D013997:Time Factors; D014779:Virus Replication
D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers; D004279:DNA, Viral; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011991:Receptors, Virus; C085301:adenovirus receptor; C018315:hexon capsid protein, Adenovirus