title: Clinical resistance to erythromycin and clindamycin in cutaneous propionibacteria isolated from acne patients is associated with mutations in 23S rRNA.
abstract: The genetic basis of erythromycin resistance in cutaneous propionibacteria was determined by comparing the nucleotide sequences of the peptidyl transferase region in the 23S rRNAs from 9 susceptible and 26 resistant clinical isolates as well as 4 laboratory-selected erythromycin-resistant mutants of a susceptible strain. In 13 isolates and the 4 laboratory mutants, cross-resistance to macrolides, lincosamides, and B-type streptogramins was associated with an A-->G transition at a position cognate with Escherichia coli 23S rRNA base 2058. These strains were resistant to > or = 512 microg of erythromycin per ml. Two other mutations were identified, an A-->G transition at base 2059 in seven strains, associated with high-level resistance to all macrolides, and a G-->A transition at base 2057 in six strains, associated with low-level resistance to erythromycin. These mutations correspond to three of four phenotypic classes previously identified by using MIC determinations.
authors.affiliation: The Skin Research Centre, Department of Microbiology, University of Leeds, United Kingdom. micjir@leeds.ac.uk
authors.forename: J I
authors.initials: JI
authors.lastname: Ross
authors.forename: E A
authors.initials: EA
authors.lastname: Eady
authors.forename: J H
authors.initials: JH
authors.lastname: Cove
authors.forename: C E
authors.initials: CE
authors.lastname: Jones
authors.forename: A H
authors.initials: AH
authors.lastname: Ratyal
authors.forename: Y W
authors.initials: YW
authors.lastname: Miller
authors.forename: S
authors.initials: S
authors.lastname: Vyakrnam
authors.forename: W J
authors.initials: WJ
authors.lastname: Cunliffe
mesh_terms: D000152:Acne Vulgaris / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics; D010458:Peptidyl Transferases / Q000235:genetics; D010641:Phenotype; D011424:Propionibacterium / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012867:Skin / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D002981:Clindamycin; D004917:Erythromycin; D010458:Peptidyl Transferases