Adjuvants influence the quantitative and qualitative immune response in BALB/c mice immunized with respiratory syncytial virus FG subunit vaccine.
The ability of monophosphoryl lipid A (MPL), QS-21 and alum to alter the immunologic response to immunization with respiratory syncytial virus a chimeric FG construct (FG) subunit vaccine was examined in BALB/c mice. FG/MPL, FG/alum, and FG/MPL/QS-21 combinations increased non-neutralizing antibody response, while FG/QS-21 did not. FG subunit vaccine with MPL, QS-21, or both had cytokine responses more closely resembling primary infection than FG/alum, with decreased interleukin-4 mRNA levels and increased IgG2a isotype antibody. The lungs of the mice immunized with FG subunit vaccines showed a heightened inflammatory response to respiratory syncytial virus challenge as compared to live virus immunization. Adjuvants can be used to alter the humoral and cellular responses to RSV subunit immunization.
Neuzil
K M
KM
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-2605, USA.
Johnson
J E
JE
Tang
Y W
YW
Prieels
J P
JP
Slaoui
M
M
Gar
N
N
Graham
B S
BS
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000534:Alum Compounds; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007114:Immunization / Q000379:methods*; D007132:Immunoglobulin Isotypes / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control*; D014765:Viral Vaccines; D014779:Virus Replication
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000914:Antibodies, Viral; D016207:Cytokines; D007132:Immunoglobulin Isotypes; D014765:Viral Vaccines; C041524:aluminum sulfate