[Fine physical mapping of yeast chromosome V].
Electrophoretic karyotype of yeast strain A364a was obtained by pulsed field gel electrophoresis and the position of chromosome V on such karyotype was determined by means of dot hybridization with chromosome V-specific probe URA3. By cloning partially digested BamHI fragments of this chromosome DNA into integrative vector Yip5, a gene library specific to this chromosome was constructed. The number of the recombinants was much more than theoretically required. After screening probe-homologous fragments from this library and analysing such fragments with restriction enzymes BamHI, EcoRI, HindIII, PstI, and SalI, a fine physical map covering about 9.4% of A364a chromosome V (which was estimated as 620kb) was constructed. Further colony hybridization with boundary clones will enable us to "walk" throughout the whole chromosome.
Yang
Z
Z
Shanghai Institute of Cell Biology, Chinese Academy of Science.
Zhu
Y
Y
Yu
Y
Y
Kuang
D
D
D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D015825:Chromosomes, Fungal; D012441:Saccharomyces cerevisiae / Q000235:genetics*