Flow cytometric analysis of lymphocytes isolated from nasal polyps.
We analyzed by flow cytometry the expression of IL-2 receptors (alpha subunit-CD25) and ICAM-1 adhesion molecules (CD54) on T cells and subsets (CD4, CD8) isolated from nasal polyp tissue in allergic and non-allergic patients. We found a significant increase in IL-2 receptor and ICAM-1 molecule expression on T cells isolated from nasal polyp tissue compared to peripheral blood lymphocytes. We also found a significantly increased expression of ICAM-1 molecules on CD8+ cells in non-allergic compared to allergic patients. The latter may reflect a difference in cytotoxic immune response between allergic and non-allergic patients, but the result should be confirmed in a more extensive study including cytokine and immunoglobulin analysis. We hope that it would enable us to obtain a deeper insight into the local immune events and further to clarify the etiology and pathogenesis of nasal polyps and their relation to allergy.
Ihan
A
A
Institute of Microbiology, Medical-Faculty, Ljubljana, Slovenia. IHAN@IBMI.MF.UNI-LJ.SI
Podboj
J
J
Suskovic
S
S
Cör
A
A
Majcen
M
M
Matos
E
E
Gale
N
N
Wraber
B
B
D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004391:Dust; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D009298:Nasal Polyps / Q000276:immunology* / Q000473:pathology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D012882:Skin Tests; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
D004391:Dust; D015375:Receptors, Interleukin-2; D018799:Intercellular Adhesion Molecule-1