title: Methods and reagents. Degraded DNA and gel tornados.
abstract: Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a case of inexplicable DNA degradation and tornados seen in agarose gels. For details on how to partake in the newsgroup, see the accompanying box.
authors.affiliation: National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA. pnh@ncifcrf.gov
authors.forename: P N
authors.initials: PN
authors.lastname: Hengen
mesh_terms: D000668:Ampicillin Resistance / Q000235:genetics; D001419:Bacteria / Q000187:drug effects / Q000235:genetics; D002021:Buffers; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D017930:Genes, Reporter; D017403:In Situ Hybridization / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D010957:Plasmids / Q000187:drug effects / Q000235:genetics; D013753:Tetracycline Resistance / Q000235:genetics
chemical_list: D002021:Buffers; D004247:DNA