Follicle-stimulating hormone activates a cAMP-dependent chloride conductance in TM4 Sertoli cells.
The effect of follicle-stimulating hormone (FSH) on the electrical properties of TM4-Sertoli cells was investigated. Addition of 5 IU/ml FSH caused a dose-dependent and reversible depolarization of the resting membrane potential by +15.3 +/- 1.0 mV accompanied by a decrease of the input resistance. The depolarization was completely abolished in chloride-free solutions. The reversal potential of the effect was close to the calculated reversal potential for chloride. We conclude that FSH activates a chloride conductance in cultured TM4 Sertoli cells.
Jungwirth
A
A
Department of Urology, General Hospital Salzburg, Austria. a.jungwirth@lkasbg.gv.at
Weiger
T
T
Singh
S K
SK
Paulmichl
M
M
Frick
J
J
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP