[Dynamics of ultrastructural morphology of the nucleolar apparatus in bovine preimplantation embryos collected in an area of chronic irradiation].
Ultrastructural morphology and immunoelectron microscopy of the nucleus and nucleologenesis in early preimplantation cow embryos were applied in an attempt to demonstrate a possible radiation injury to that early stage of development due to chronical irradiation of the animals in the Tchernobyl area. Mostly eight cell embryos as well as morulae were collected from superovulated cows which were previously constantly kept in zones of different levels of radioactive irradiation. In addition to the normometric status of reproductive organs in no case was it possible to detect an apparent deviation in the nuclear morphology or in the process of nucleologenesis as compared to the physiological situation (Kopecný et al., 1989b, 1991, 1996). This observation was supported by an immunoelectron microscope study of DNA association and penetration in the differentiated nucleolus in the late 8-cell stage. These observations show that the otherwise demonstrated radiation injury localized in the genome does not probably influence markedly the early events of the developing embryo and that the aberrant cytoplasmic command of the nuclear events known in other types of oocyte/early cow embryo impairment (review Kopecný and Nicmann, 1993; Kanka et al. 1991; Pavlok et al., 1993) is not seen in early embryos collected from chronically irradiated animals.
Pivko
J
J
Research Institute of Animal Production, Nitra, Slovak Republic.
Baran
V
V
Grafenau
P
P
Kopecný
V
V
Pelechatyj
N S
NS
Bondarcuk
V N
VN
Kozuch
A J
AJ
Kovalcik
L M
LM
D000818:Animals; D001755:Blastocyst / Q000528:radiation effects* / Q000648:ultrastructure*; D002417:Cattle; D002466:Cell Nucleolus / Q000528:radiation effects* / Q000648:ultrastructure*; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D018788:Radioactive Hazard Release; D014455:Ukraine
Dynamická ultramikromorfológia nukleolárneho aparátu preimplantacných embryí kráv z oblasti chronického rádioaktívneho oziarenia.