Population studies of human deoxyribonuclease I polymorphism.
We have improved the resolution of the conventional method for phenotyping deoxyribonuclease I (DNase I), which makes use of isoelectric focusing, by the addition of amphoteric separators. The distribution of DNase I phenotypes was extensively examined using this improved method in 1,212 unrelated individuals from a Japanese population. In order to investigate a possible difference in phenotype distribution between different populations, DNA samples from Germans and African Americans were genotyped using the polymerase chain reaction. The DNASE1*2 allele in the German population was found to be predominant among the four alleles of DNase I, in contrast to the Japanese population. These results are the first to demonstrate a wide distribution of DNase I polymorphism in the Japanese population as well as in two other populations.
Yasuda
T
T
Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Takeshita
H
H
Iida
R
R
Yuasa
I
I
Kishi
K
K
D044466:Asian People / Q000235:genetics; D044383:Black People / Q000235:genetics; D003850:Deoxyribonuclease I / Q000235:genetics* / Q000652:urine; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D044465:White People / Q000235:genetics; D001741:Black or African American
D003850:Deoxyribonuclease I