title: [Rheologic study of the dissociation reaction of erythrocyte immunologic agglutinates: application to subgroup A erythrocytes].
abstract: Red blood cell Agglutinates can be dissociated by providing enough energy which may be supplied by shear stress in a viscometer. In this paper a new technique is proposed to determine dissociation parameters of erythrocyte immunological agglutinates by using a laser backscattering method. Backscattered light intensity increases during the process of agglutinate dissociation when a controlled shear stress in a transparent Couette viscometer is applied. The obtained curves of dissociation can be fitted by an exponential function. A numerical integration of the dissociation curves allows us to obtain a parameter associated to the energy used in the dissociation process. A satisfactory differentiation of erythrocyte sub-groups Al, Az, A,B and Am has been carried out using this technique.
authors.affiliation: Laboratorio de Immunohematología, Hemorreología e Histocompatibilidad, Fac. de Cs. Bioquim. y Farmac., Rosario, Argentine.
authors.forename: J R
authors.initials: JR
authors.lastname: Rasia-Valverde
authors.forename: R J
authors.initials: RJ
authors.lastname: Rasia
authors.forename: C
authors.initials: C
authors.lastname: Botte
authors.forename: J F
authors.initials: JF
authors.lastname: Stoltz
mesh_terms: D001809:Blood Viscosity; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D007834:Lasers; D037102:Lectins; D016015:Logistic Models; D015203:Reproducibility of Results; D012212:Rheology; D012542:Scattering, Radiation; D013314:Stress, Mechanical
chemical_list: D037102:Lectins
vernacular_title: Approche rhéologique de la réaction de dissociation d'agglutinats immunologiques érythrocytaires: application à des hématies du sous-groupe A.