Adherence of Borrelia burgdorferi to granulocytes of different animal species.
Adherence of 4 Borrelia (B.) burgdorferi strains (z7/22, z7/27, z7/41, PBi) to polymorphonuclear granulocytes from different domestic animals (horses, cattle, sheep, dogs) was investigated. All 4 strains adhered to the granulocytes. Binding assays indicated that the adherence occurred between structures on the surface of the borreliae ("binding-sites") and on the membranes of the granulocytes ("receptors"). The "receptors" consisted of 4 fractions (A, B, C, and D) with components differing in molecular weight (MW) and binding activity for proteins on the surface of B. burgdorferi. Fraction A (MW 80000) had the highest binding activity for B. burgdorferi.
Grassmann
B
B
Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen, Germany.
Kopp
P A
PA
Schmitt
M
M
Blobel
H
H
D000818:Animals; D001422:Bacterial Adhesion; D015748:Borrelia burgdorferi Group / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D003902:Detergents / Q000494:pharmacology; D004285:Dogs; D006098:Granulocytes / Q000166:cytology / Q000382:microbiology*; D006361:Heating; D006736:Horses; D006801:Humans; D017830:Octoxynol / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism; D012756:Sheep; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014105:Tosyl Compounds / Q000494:pharmacology
D003902:Detergents; D011956:Receptors, Cell Surface; D014105:Tosyl Compounds; D012967:Sodium Dodecyl Sulfate; C003614:4-toluenesulfonyl fluoride; D017830:Octoxynol