The role of retinotomy in an experimental rabbit model of proliferative vitreoretinopathy.
OBJECTIVE
To create a model of proliferative vitreoretinopathy (PVR) using retinotomy with vitrectomy, cryotherapy, and platelet-rich plasma (PRP) injection, which more closely resembles the human pathophysiologic condition.


METHODS
One hundred and twenty albino rabbits were divided into 10 groups of 12 rabbits each and underwent the following procedures: group 1, vitrectomy; group 2, cryotherapy; group 3, PRP intravitreous injection; group 4, retinotomy; group 5, retinotomy and vitrectomy; group 6, retinotomy and cryotherapy; group 7, retinotomy and PRP injection; group 8, retinotomy, vitrectomy, and cryotherapy; group 9, vitrectomy, cryotherapy, and PRP injection and group 10, retinotomy, vitrectomy, cryotherapy, and PRP injection. All animals underwent follow-up examinations with indirect ophthalmoscopy and fundus photography on days 1, 3, 7, 10, 14, 21, and 28 after the procedure(s). Retinal changes were categorized according to the classification of Fastenberg et al. At the end of the experiments, the eyes were enucleated, and examined under light and electron microscopy.


RESULTS
No retinal detachments (RDs) were observed in groups 1, 2, 4, 5, 6, and 8. RDs of varying severity were observed in group 3 (n = 1), group 7 (n = 2), group 9 (n = 6), and group 10 (n = 12). Light and transmission electron microscopy confirmed the findings.


CONCLUSIONS
Combining retinotomy with vitreous removal, cryotherapy, and PRP injection creates an efficient and different model of PVR that produced RD in 100% of rabbit eyes.
Goldaracena
M B
MB
Department of Biology and Histology, Facultad de Medicina, Universidad de Valladolid, Spain.
Garcia-Layana
A
A
Pastor
J C
JC
Saornil
M A
MA
de la Fuente
F
F
Gayoso
M J
MJ
D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D017679:Cryotherapy; D004195:Disease Models, Animal; D005654:Fundus Oculi; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D007267:Injections; D008854:Microscopy, Electron; D009887:Ophthalmoscopy; D010949:Plasma / Q000166:cytology / Q000502:physiology; D011183:Postoperative Complications; D011817:Rabbits; D012160:Retina / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D012163:Retinal Detachment / Q000209:etiology / Q000473:pathology; D014746:Vimentin / Q000378:metabolism; D014821:Vitrectomy; D018630:Vitreoretinopathy, Proliferative / Q000209:etiology*
D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin