title: Daleformis, a new phytoalexin from the roots fo Dalea filiciformis: an inhibitor of endothelin converting enzyme.
abstract: As part of a search for novel inhibitors of endothelin converting enzyme (ECE), the MeOH-CH2Cl2 extract of the roots of Dalea filiciformis was shown to be active. Bioassay-guided fractionation of the extract yielded a novel phytoalexin, daleformis (1), whose structure was determined by interpretation of spectral data and X-ray analysis. Daleformis (1) inhibited ECE with an IC50 of 9 microM.
authors.affiliation: Department of Biomolecular Discovery and Analytical Sciences, SmithKline Beecham Pharmaceuticals, Research and Development, King of Prussia, Pennsylvania 19406-0939.
authors.forename: A D
authors.initials: AD
authors.lastname: Patil
authors.forename: A J
authors.initials: AJ
authors.lastname: Freyer
authors.forename: D S
authors.initials: DS
authors.lastname: Eggleston
authors.forename: R C
authors.initials: RC
authors.lastname: Haltiwanger
authors.forename: B
authors.initials: B
authors.lastname: Tomcowicz
authors.forename: A
authors.initials: A
authors.lastname: Breen
authors.forename: R K
authors.initials: RK
authors.lastname: Johnson
mesh_terms: D000107:Acetylation; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors*; D001572:Benzofurans / Q000302:isolation & purification* / Q000494:pharmacology; D001578:Benzopyrans / Q000302:isolation & purification* / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D018360:Crystallography, X-Ray; D000072396:Endothelin-Converting Enzymes; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D008968:Molecular Conformation; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
chemical_list: D001572:Benzofurans; D001578:Benzopyrans; D004791:Enzyme Inhibitors; C105641:daleformis; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; D000072396:Endothelin-Converting Enzymes