title: p53/58 binds COPI and is required for selective transport through the early secretory pathway.
abstract: p53/58 is a transmembrane protein that continuously recycles between the ER and pre-Golgi intermediates composed of vesicular-tubular clusters (VTCs) found in the cell periphery and at the cis face of the Golgi complex. We have generated an antibody that uniquely recognizes the p53/58 cytoplasmic tail. Here we present evidence that this antibody arrests the anterograde transport of vesicular stomatitis virus glycoprotein and leads to the accumulation of p58 in pre-Golgi intermediates. Consistent with a role for the KKXX retrieval motif found at the cytoplasmic carboxyl terminus of p53/58 in retrograde traffic, inhibition of transport through VTCs correlates with the ability of the antibody to block recruitment of COPI coats to the p53/58 cytoplasmic tail and to p53/58-containing membranes. We suggest that p53/58 function may be required for the coupled exchange of COPII for COPI coats during segregation of anterograde and retrograde transported proteins.
authors.affiliation: The Scripps Research Institute, Department of Cell Biology, La Jolla, California 92037, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Tisdale
authors.forename: H
authors.initials: H
authors.lastname: Plutner
authors.forename: J
authors.initials: J
authors.lastname: Matteson
authors.forename: W E
authors.initials: WE
authors.lastname: Balch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D018699:Coated Vesicles / Q000378:metabolism*; D020756:Coatomer Protein; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism*; D005089:Exocytosis; D019084:Fluorescent Antibody Technique, Indirect; D006056:Golgi Apparatus / Q000378:metabolism*; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011485:Protein Binding; D051381:Rats; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: D020756:Coatomer Protein; C028391:G protein, vesicular stomatitis virus; C501458:Lman1 protein, rat; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D010455:Peptides; D014759:Viral Envelope Proteins