[Rapid isolation of 4-hydroxyphenylethanol from the bovine brain using Water Sep-Pak Plus cartridges with lipophilic sorbent (Florisil)].
4-Hydroxypherlylethanol, MAO-A Inhibitory component of endogenous MAO Inhibitory activity, tribulin, was isolated from a small quantity of bovine brain using Maters Sep-Pak Florisil cartridges. Procedure included isolation of crude tribulin fraction, which was passed through the cartridge. Preliminary data revealed that this substance is not absorbed on Florisil. The employment of this type of cartridges essentially accelerates isolation of 4-hydroxyphenyethanol from brain samples for quantitative determination. Pilot experiments also revealed that Sep-Pak Folrisil cartridges could be used for the rapid isolation of isatin, another endogenous MAO (B) inhibitor, from brain tissue.
Panova
N G
NG
Veselovskaia
N V
NV
Medvedev
A E
AE
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation*; D017633:Magnesium Silicates; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000302:isolation & purification
D017633:Magnesium Silicates; C005013:Florisil; C011867:4-hydroxyphenylethanol; D010626:Phenylethyl Alcohol
Bystroe vydelenie 4-gidroksifenilétanola iz mozga byka c ispol'zovaniem Waters Sep-Pak Plus kartridzheĭ s lipofil'nym sorbentom (Florisil).