A micro-dissection approach for isolation of NotI linking clones from regions frequently deleted in RCC and SCLC.
We demonstrate that micro-dissection can be used for isolating NotI linking clones from the human 3p21-pter region. This approach is an improvement to positional cloning techniques, since NotI linking clones are directly linked with genes.
Alimov
A A
AA
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. Andrei.Alimov@mtc.ki.se
Rodova
M A
MA
Gizatullin
R Z
RZ
Kost-Alimova
M Y
MY
Fedorova
L I
LI
Barsky
V E
VE
Kashuba
V
V
Klein
G
G
Zabarovsky
E R
ER
Zelenin
A V
AV
D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular / Q000379:methods*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D008846:Micromanipulation / Q000379:methods*; D008969:Molecular Sequence Data
D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases