title: Inhibitory effect of neuraminidase on SP-induced histamine release and TNF-alpha mRNA in rat mast cells: evidence of a receptor-independent mechanism.
abstract: The neuropeptide substance P (SP) is a mediator of neuro-inflammation and can play a role by induction of histamine release (HR) and TNF-alpha. However, its effect on the heterogeneous response of mast cells (MC) has not been completely studied. We have established that the SR can induce 25% of HR in highly purified rat uterine MC at diestrous but not at proestrous phases of the reproductive cycle and 88% of HR in peritoneal mast cells (PMC). We also found 2.2 fold increase in TNF-alpha mRNA at diestrous, in SP stimulated uterine MC versus control and 2.7 fold increase in PMC; RT and competitive PCR were used to amplify the TNF-alpha mRNA. We have thereafter investigated the mechanism whereby the binding of SP to sialic acid on the MC membrane, could trigger secretion of histamine and induction of TNF-alpha mRNA. The neuraminidase pretreatment (0.1 U/ml) inhibited SP-stimulated HR from either uterine MC and PMC (98% and 50%, respectively) and totally inhibited SP-stimulated TNF-alpha mRNA levels. The neuraminidase effect was not toxic, since it was not observed in IgE mediated HR and TNF-alpha mRNA levels. In conclusion, the inhibitory effect of the neuraminidase on the SP-mediated increase of histamine and TNF-alpha mRNA, suggests that the SP-sialic acid interaction could have a role in the MC heterogeneous response.
authors.affiliation: Istituto di Biologia dello Sviluppo, CNR, Palermo, Italy. geraci@mbox.unipa.it
authors.forename: R
authors.initials: R
authors.lastname: Cocchiara
authors.forename: A
authors.initials: A
authors.lastname: Bongiovanni
authors.forename: G
authors.initials: G
authors.lastname: Albeggiani
authors.forename: A
authors.initials: A
authors.lastname: Azzolina
authors.forename: N
authors.initials: N
authors.lastname: Lampiasi
authors.forename: F
authors.initials: F
authors.lastname: Di Blasi
authors.forename: D
authors.initials: D
authors.lastname: Geraci
mesh_terms: D000818:Animals; D005260:Female; D006633:Histamine Antagonists / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology*; D010537:Peritoneum / Q000166:cytology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000502:physiology; D013373:Substance P / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014599:Uterus / Q000166:cytology
chemical_list: D006633:Histamine Antagonists; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; D013373:Substance P; D009439:Neuraminidase