Thymopentin loaded microsphere preparation by w/o/w emulsion technique: in vitro/ex vivo evaluation.
Poly-D,L-lactide (PDLLA) and polylactide-co-glycolide (PLGA) microspheres containing thymopentin have been prepared by a water-in-oil-in-water-emulsion/solvent evaporation technique. The goal is to stabilize the active compound thymopentin, and to prolong its therapeutic activity, by embedding the drug in a polymeric matrix. The microspheres obtained have been characterized for their morphology and drug content. In-vitro dissolution tests have been performed on the microspheres. Results show that the type of polymer employed (PDLLA or PLGA) does not seem to affect microsphere morphology, while in-vitro dissolution profiles are greatly influenced by the composition of polymer matrix. Ex-vivo evaluation of PLGA microspheres performed on mouse thymocites shows that biological activity of Thymopentin is maintained after loading into PLGA microspheres.
Conti
B
B
Istituto di Chimica Farmaceutica, Università di Catalina, Italia.
Panico
M
M
Ventura
C A
CA
Giunchedi
P
P
Puglisi
G
G
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D002152:Calorimetry, Differential Scanning; D002214:Capsules; D004339:Drug Compounding; D004655:Emulsions; D019344:Lactic Acid; D008213:Lymphocyte Activation / Q000187:drug effects; D011091:Polyesters; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D012995:Solubility; D013601:T-Lymphocytes / Q000187:drug effects; D016305:Thymopentin / Q000008:administration & dosage*
D000276:Adjuvants, Immunologic; D002214:Capsules; D004655:Emulsions; D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D016305:Thymopentin