D1 dopamine receptors are not expressed in human melanoma.
D1 dopamine receptor mRNA has been demonstrated in mouse melanoma cells, and the expression of these G-protein-coupled receptors in human melanoma was therefore presumed when dopamine receptor binding radiopharmaceuticals were found to be useful for the detection of metastases in whole-body scintigraphy. The aim of this study was thus to investigate if D1 dopamine receptor mRNA or protein could be directly demonstrated in melanoma cells. The presence of D1 dopamine receptor mRNA was investigated in six human melanoma cell lines from metastases using reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, in vitro binding assays with the D1 dopamine receptor agonist 125I-Sch 23982 were performed in 19 melanoma metastases. No D1 dopamine receptor mRNA could be detected by RT-PCR. All melanotic metastases were found to accumulate 125I-Sch 23982, with the presence of binding sites and intensity of 125I-Sch 23982 labelling correlating to the amount of melanin present in the metastases. Two amelanotic melanomas did not accumulate 125I-Sch 23982. D1 dopamine receptors could not be detected by means of RT-PCR or in vitro binding assays in human melanomas. Detection of antagonists is best explained by non-specific binding to melanin.
Böni
R
R
Department of Dermatology, University Hospital of Zürich, Switzerland.
Lichtensteiger
W
W
Steinert
H C
HC
Böni
R A
RA
Früh
H
H
Dummer
R
R
Hartmann
D G
DG
Laine
E
E
Burg
G
G
D000818:Animals; D017931:DNA Primers; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D017447:Receptors, Dopamine D1 / Q000032:analysis / Q000096:biosynthesis*; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D017447:Receptors, Dopamine D1