title: Short-term ozone exposure upregulates P-selectin in normal human airways.
abstract: Short-term exposure to ambient levels of ozone induces neutrophilic bronchitis. To investigate the early events contributing to inflammatory cell recruitment in the airways we exposed 12 healthy nonsmoking volunteers to 0.12 ppm ozone or filtered air for 2 h on two separate occasions. Spirometry and fiberoptic bronchoscopy were performed immediately and at 1.5 h after the two exposures, respectively. Total protein, albumin, and total and differential cell counts were performed on the bronchial wash and BAL fluid. Bronchial biopsies were embedded in glycol methacrylate and immunostained for inflammatory cells, including neutrophils, mast cells, total T-cells (CD3), T-cell subset CD8, and leukocyte endothelial adhesion molecules, including VCAM-1, ICAM-1, E-selectin, and P-selectin. No significant changes were observed in FEV1, FVC, or any inflammatory indices in the bronchial wash and BAL fluid. In addition, no significant differences were seen in inflammatory cell numbers or percentages of vessels expressing VCAM-1, E-selectin, or ICAM-1 in the biopsies. The percentage of vessels expressing P-selectin increased significantly after ozone exposure: p = 0.016; median (IQR), 28.76 (26.36-36.94) versus 47.06 (38.14-56.86)%. The upregulation of P-selectin could signify an early inflammatory response to ozone such as margination and rolling of the neutrophils on the vessel wall prior to transendothelial migration.
authors.affiliation: Air Pollution Research Group, University Medicine, Southampton General Hospital, United Kingdom.
authors.forename: M T
authors.initials: MT
authors.lastname: Krishna
authors.forename: A
authors.initials: A
authors.lastname: Blomberg
authors.forename: G L
authors.initials: GL
authors.lastname: Biscione
authors.forename: F
authors.initials: F
authors.lastname: Kelly
authors.forename: T
authors.initials: T
authors.lastname: Sandstrom
authors.forename: A
authors.initials: A
authors.lastname: Frew
authors.forename: S
authors.initials: S
authors.lastname: Holgate
mesh_terms: D000328:Adult; D001706:Biopsy; D001980:Bronchi / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D001999:Bronchoscopy; D002452:Cell Count; D019040:E-Selectin / Q000378:metabolism; D005260:Female; D005541:Forced Expiratory Volume / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008297:Male; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D019007:P-Selectin / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D013147:Spirometry; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D014797:Vital Capacity / Q000187:drug effects
chemical_list: D019040:E-Selectin; D010083:Oxidants, Photochemical; D019007:P-Selectin; D011506:Proteins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D010126:Ozone