Effect of microwaves on survival of some bacterial strains.
While the inhibitory effect of microwave radiation on microorganisms is being researched intensively, how microwave radiation brings about this effect has been a matter of discussion. Some researchers support that this effect is of a thermal character, whereas some others maintain a non-thermal effect. In this work, 1 ml suspensions of Pseudomonas aeruginosa, Pseudomonas acidovorans staphylococcus aureus and Staphylococcus epidermidis bacteria were subjected to microwave radiation at 2450 MHz and 550 Watts for periods of 5, 6, 7, 8, 10, 12, 14, 16, 18, 20, 25 and 30 seconds. When each result was compared with the CFU/ml results obtained from unradiated control group bacterial suspensions derived from stock cultures, significant conclusions were attained (P < 0.001). The same experiments were repeated with the application of conventional heating. The difference between the CFU/ml values of similar bacterial suspensions subjected to microwave radiation and conventional heating was significant (P < 0.001). Concurrently, the fact that the effect was exacerbated upon increasing of liquid volume during the application of microwave radiation was established via the results obtained through the application of microwave radiation to 1 ml and 5 ml bacterial suspensions (P < 0.001).
Atmaca
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Department of Microbiology and Biophysics, Medical Faculty, University of Dicle, Diyarbakir, Turkey.
Akdag
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Dasdag
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Celik
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D015169:Colony Count, Microbial; D003470:Culture Media; D008872:Microwaves; D011549:Pseudomonas / Q000254:growth & development / Q000528:radiation effects*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000528:radiation effects*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000528:radiation effects*; D013212:Staphylococcus epidermidis / Q000254:growth & development / Q000528:radiation effects*
D003470:Culture Media