Anti-mitosin antibodies in a patient with chronic graft-versus-host disease after allogeneic bone marrow transplantation.
In a patient undergoing allogeneic BMT for ALL, chronic GVHD (cGVHD) with skin changes developed within 110 days after transplantation. One year post-BMT, anti-nuclear antibodies were detected. The patient's serum was used for immunoscreening of a HeLa cDNA library. Ten different overlapping positive clones were found to be partial clones of mitosin, a 350-kDa nuclear phosphoprotein which shows a speckled nuclear distribution in S phase and which relocates to the centromere and mitotic apparatus in M phase. Although autoantibodies against centromere protein-F, which is very similar to mitosin, have been reported in patients with cancer, this is the first report of autoantibodies against mitosin in a patient with cGVHD.
Muro
Y
Y
Department of Dermatology, Nagoya University School of Medicine, Japan.
Kamimoto
T
T
Hagiwara
M
M
D000328:Adult; D000974:Antibodies, Antinuclear / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D002868:Chromosomal Proteins, Non-Histone; D006086:Graft vs Host Disease / Q000097:blood / Q000276:immunology*; D006801:Humans; D008297:Male; D008840:Microfilament Proteins; D009687:Nuclear Proteins / Q000276:immunology*; D010750:Phosphoproteins / Q000276:immunology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy*
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D002868:Chromosomal Proteins, Non-Histone; D008840:Microfilament Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; C094772:centromere protein F