Mouse cerebellar adenosinergic modulation of ethanol-induced motor incoordination: possible involvement of cAMP.
As an extension of our previous work pertaining to brain adenosinergic modulation of ethanol-induced motor incoordination, the effect of direct intracerebellar administration of the A1-selective adenosine agonist, N6-cyclohexyladenosine (CHA) on ethanol-induced motor incoordination was evaluated. Marked accentuation of ethanol-induced motor impairment by CHA was observed. No change in the normal motor coordination was noted when CHA administration was followed by saline instead of ethanol. Intracerebellar cAMP or its analog, 8-(4-chlorophenylthio)-cAMP, significantly inhibited ethanol's motor impairment in a dose-related manner as well as abolished CHA's accentuating effect on ethanol-induced motor incoordination. These observations suggested a possible involvement of cAMP in the adenosinergic modulation and in the expression of ethanol-induced motor incoordination. Further support was provided by the observation of a marked accentuation and attenuation in a dose-related manner of ethanol-induced motor impairment as well as CHA's accentuation of ethanol's motor impairment by intracerebellar miconazole and forskolin, respectively. However, equimolar intracerebellar doses of miconazole and forskolin (inhibitor and stimulator of adenylyl cyclase, respectively) failed to significantly alter ethanol-induced motor incoordination probably due to their mutual functional antagonism. The expression of adenosinergic modulation and that of ethanol-induced motor impairment most likely involved Gi protein-coupled receptor(s) (such as adenosine receptors). The involvement of receptors linked to pertussis toxin-sensitive G-proteins was suggested because intracerebellar pertussis toxin pretreatment markedly inhibited ethanol-induced motor incoordination as well as CHA's accentuation of ethanol's motor impairment. Finally, cAMP, unlike its antagonism to CHA's accentuation, failed to antagonize the accentuation of ethanol-induced motor impairment by intracerebellar GABA(A) agonist (+)-muscimol. This indicated selectivity of cAMP participation in G protein coupled receptor (such as adenosine)-mediated response and not in ionic channel coupled receptor (such as GABA(A))-mediated mechanism. Overall, the data suggested a possible involvement of cerebellar adenylyl cyclase-cAMP signalling pathway in the adenosinergic modulation of ethanol's ataxia.
Dar
M S
MS
Department of Pharmacology, School of Medicine, East Carolina University, Greenville 27858, USA.
D000241:Adenosine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D037361:Adenylate Cyclase Toxin; D000704:Analysis of Variance; D000818:Animals; D002525:Cerebellar Cortex / Q000187:drug effects / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D007263:Infusions, Parenteral; D008297:Male; D051379:Mice; D008825:Miconazole / Q000008:administration & dosage / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects*; D037342:Pertussis Toxin; D058906:Purinergic P1 Receptor Agonists; D018047:Receptors, Purinergic P1 / Q000502:physiology*; D013873:Thionucleotides / Q000494:pharmacology; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D058906:Purinergic P1 Receptor Agonists; D018047:Receptors, Purinergic P1; D013873:Thionucleotides; D010566:Virulence Factors, Bordetella; D005576:Colforsin; C027513:N(6)-cyclohexyladenosine; D000431:Ethanol; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D008825:Miconazole; D000242:Cyclic AMP; D037342:Pertussis Toxin; D000241:Adenosine