Establishment of a new cell line from a patient with hairy cell leukemia-Japanese variant.
A cell line, JHC-2, was established from the peripheral blood of a patient with hairy cell leukemia (HCL)-Japanese variant. The JHC-2 cells have cytologic features similar to those of the original tumor cells. They displayed hairy cytoplasmic projections by phase contrast and scanning electron microscopy. The tartrate-resistant acid phosphatase reaction was weakly positive. The immunophenotype of the JHC-2 cells was CD5-, CD10-, CD11c+/-, CD19+, CD21+, CD23+, CD24-, CD25+/-, CD38- and FMC-7+. The expression of surface immunoglobulin (IgG, kappa) and the configuration of Ig gene rearrangements in the JHC-2 cells were identical to those in the original leukemic cells, and the JHC-2 cells displayed trisomy 9 on cytogenetic examination. Southern blot analysis for the Epstein-Barr virus (EBV) genome showed that the JHC-2 cells contained the EBV genome, although the freshly isolated leukemic cells did not. These results indicate that the JHC-2 cell line is an EBV spontaneously transformed B cell line originating from HCL cells.
Shibayama
H
H
Department of Hematology, Osaka University Medical School, Japan.
Machii
T
T
Tokumine
Y
Y
Nishimori
Y
Y
Nojima
J
J
Inoue
R
R
Kanamaru
A
A
Tagawa
S
S
Kitani
T
T
D000135:Acid Phosphatase / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D015321:Gene Rearrangement; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics* / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D007564:Japan; D007943:Leukemia, Hairy Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000954:Antigens, Surface; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007527:Isoenzymes; D015375:Receptors, Interleukin-2; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase