title: Production of env-deficient rous sarcoma virus (RSV) early after infection.
abstract: Production of defective virus particles during the early stage of Rous sarcoma virus (RSV) infection of chicken embryo fibroblasts (CEF) was examined. RSV harvested 2 days post infection (2pi) had 10 to 30 times lower specific infectivity (focus forming units/unit reverse transcriptase activity) than 5pi harvest. Virus particles produced on day 2 contained less env proteins than particles harvested on day 5. The amount of other viral proteins was equal in particles harvested on day 2 and day 5. Analysis of infected cells revealed that these cells synthesized less env proteins on day 2 than on day 5. RSV RNA in infected cells was spliced normally on day 2. Infection at a low multiplicity of infection (moi) prolonged the production of defective particles. When infection was initiated by a low moi (0.01), particles harvested on day 5 had the same characteristics as 2pi particles after infection with a high moi (1.0). We conclude that the low infectivity of early harvest is due to the reduced amount of env proteins in virus particles, which is a consequence of the reduced env protein synthesis.
authors.affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
authors.forename: H
authors.initials: H
authors.lastname: Hara
authors.forename: H T
authors.initials: HT
authors.lastname: Park
authors.forename: M
authors.initials: M
authors.lastname: Fujihara
authors.forename: A
authors.initials: A
authors.lastname: Kaji
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D002642:Chick Embryo; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology*; D005347:Fibroblasts; D015686:Gene Products, env / Q000096:biosynthesis*; D015752:Genes, env; D016391:Genes, src; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis; D014774:Virulence; D014779:Virus Replication
chemical_list: D015686:Gene Products, env; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase