Induction of early pregnancy factor activity in vitro by platelet-activating factor in mice.
The rosette inhibition test was used to determine early pregnancy factor activity in culture media from oestrous mouse ovaries and oviducts stimulated in vitro for early pregnancy factor production under different experimental conditions. Embryo conditioned media, platelet-activating factor and cortical granule release media could all stimulate the production of early pregnancy factor by oestrous mouse ovaries and oviducts. This stimulation was completely blocked by the presence of BN 52021, a platelet-activating factor receptor antagonist. This study indicates that platelet-activating factor is the 'ovum factor' released by the zygote on fertilization to initiate the synthesis of early pregnancy factor.
Lash
G E
GE
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Legge
M
M
D000818:Animals; D018835:Chaperonin 10; D017077:Culture Media, Conditioned; D046508:Culture Techniques; D004224:Diterpenes; D005187:Fallopian Tubes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D046934:Ginkgolides; D007166:Immunosuppressive Agents; D007783:Lactones / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D010980:Platelet Membrane Glycoproteins / Q000187:drug effects; D011257:Pregnancy Proteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D013268:Stimulation, Chemical; D013491:Suppressor Factors, Immunologic
D018835:Chaperonin 10; D017077:Culture Media, Conditioned; D004224:Diterpenes; D046934:Ginkgolides; D007166:Immunosuppressive Agents; D007783:Lactones; D010455:Peptides; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011257:Pregnancy Proteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D013491:Suppressor Factors, Immunologic; C025124:early pregnancy factor; C039351:platelet activating factor receptor; C045856:ginkgolide B