Localization of the gene for the human serotonin 5-HT(2B) receptor to chromosome 2.
A 483 bp Bgl II/Sal I repeat free fragment was isolated from the vector containing the human serotonin 5-HT(2B) receptor. This fragment contained part of the coding sequence located within the last exon and the entire 3'-non-translated region. It was used to probe, by Southern blots, Pst I digested DNA from a series of 20 rodent-human somatic cell hybrid lines, each of which contained different complements of human chromosomes. This 5-HT(2B) receptor probe showed hybridization to a 2-7 kb Pst I fragment which revealed 100 percent concordance with location of the gene for this 5-HT receptor variant at chromosome 2.
Horton
Y M
YM
Molecular Pharmacology Group, University of Glasgow, Scotland, UK.
Lubbert
H
H
Houslay
M D
MD
D000818:Animals; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D006224:Cricetinae; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D044403:Receptor, Serotonin, 5-HT2B; D011985:Receptors, Serotonin / Q000235:genetics* / Q000378:metabolism
D015342:DNA Probes; D044403:Receptor, Serotonin, 5-HT2B; D011985:Receptors, Serotonin; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific