title: Antimonite is accumulated by the glycerol facilitator GlpF in Escherichia coli.
abstract: In a search for genes responsible for the accumulation of antimonite in Escherichia coli, TnphoA was used to create a pool of random insertional mutants, from which one antimonite-resistant mutant was isolated. Sequence analysis showed that the TnphoA insertion was located in the glpF gene, coding for the glycerol facilitator GlpF. The mutant was shown to be defective in polyol transport by GlpF. These results suggest that in solution Sb(III) is recognized as a polyol by the glycerol facilitator.
authors.affiliation: Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
authors.forename: O I
authors.initials: OI
authors.lastname: Sanders
authors.forename: C
authors.initials: C
authors.lastname: Rensing
authors.forename: M
authors.initials: M
authors.lastname: Kuroda
authors.forename: B
authors.initials: B
authors.lastname: Mitra
authors.forename: B P
authors.initials: BP
authors.lastname: Rosen
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D000965:Antimony / Q000378:metabolism*; D020346:Aquaporins; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005990:Glycerol / Q000378:metabolism*; D016254:Mutagenesis, Insertional
chemical_list: D020346:Aquaporins; D001425:Bacterial Outer Membrane Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C078043:GlpF protein, E coli; C061374:antimonite; D000965:Antimony; D000469:Alkaline Phosphatase; D005990:Glycerol