title: Multiple fucosyltransferases and their carbohydrate ligands are involved in spermatogenic cell-Sertoli cell adhesion in vitro in rats.
abstract: We have identified multiple fucosyltransferases (FTs) (alpha[1-2]-, alpha[1-3]-, alpha[1-4]-FTs) on cells of the rat seminiferous epithelium as demonstrated by fucose incorporation into phenyl-beta-D-galactoside (Ph-beta-D-Gal), 2'-fucosyllactose (2'-FL), and lacto-N-fucopentaose-l (LNF I), respectively. Now, using fluorescence laser scanning cytometry, we report that multiple FTs are implicated in germ cell-Sertoli cell adhesion in vitro. Sertoli cells were isolated from 19- to 21-day-old CD rats and cultured for 6-10 days. Mixed germ cells were obtained by enzymatic dispersion of adult rat testis and cultured overnight before labeling with 10 microM acetoxymethyl ester derivative of the fluorescent indicator, calcein. The adherent cell analysis and sorting 570 interactive laser cytometer was used to determine the number of labeled adherent germ cells on Sertoli cell monolayers in the presence or absence of a variety of low molecular weight acceptors for fucose. Coincubation of labeled germ cells with Sertoli cell monolayers in the presence of GDP-fucose, UDP-galactose, Ph-beta-D-Gal, 2'-FL, LNF I, and Lewis-X and 3'-sialyl-Lewis-X oligosaccharides resulted in significant reduction of germ cell binding when compared to that of the untreated controls or of control samples incubated with cellobiose, melibiose, and alpha-D-mannopyranose, which do not serve as fucose acceptors. Our results suggest that multiple FTs and their lectin/selectin ligands are involved in mediating germ cell-Sertoli cell adhesion to form a cohesive epithelium and thus aid germ cell adluminal translocation within the seminiferous epithelium.
authors.affiliation: Department of Cell Biology and Neuroscience, University of South Carolina School of Medicine, Columbia 29208, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Raychoudhury
authors.forename: C F
authors.initials: CF
authors.lastname: Millette
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002448:Cell Adhesion / Q000502:physiology; D002469:Cell Separation; D002478:Cells, Cultured; D005434:Flow Cytometry; D005643:Fucose / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism*; D066298:In Vitro Techniques; D008024:Ligands; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*
chemical_list: D008024:Ligands; D005643:Fucose; D005647:Fucosyltransferases