title: Cloning and characterization of TDD5, an androgen target gene that is differentially repressed by testosterone and dihydrotestosterone.
abstract: By using mRNA polymerase chain reaction differential display technique (DDPCR), we have identified one early responsive cDNA fragment, TDD5, from a 5alpha-reductase-deficient T cell hybridoma. The DDPCR profiles of TDD5 suggest that its expression can be repressed by testosterone (T) within 2 hr. More importantly, both DDPCR and Northern blot analysis further demonstrated that the expression of TDD5 was differentially repressed by T and dihydrotestosterone (DHT) at the mRNA level. To our knowledge, this is the first androgen target gene to show a preference in response to T over DHT in cell culture. TDD5 is expressed in several tissues with particular abundance in kidney. Full-length TDD5 cDNA (2,916 bp) encodes a protein with a calculated molecular weight of 42,000. Finally, our animal studies further confirm that TDD5 mRNA levels can be repressed to the basal level 8 hr after DHT administration. The isolation and characterization of the early-responsive androgen target gene TDD5 and the fact that TDD5 mRNA level can be differentially regulated by T and DHT may provide a useful tool to study the molecular mechanism of androgen preference on target gene regulation.
authors.affiliation: Department of Medicine, University of Wisconsin Comprehensive Cancer Center, University of Wisconsin, Madison, WI 53792, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Lin
authors.forename: C
authors.initials: C
authors.lastname: Chang
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000172:deficiency; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D013196:Dihydrotestosterone / Q000494:pharmacology*; D006825:Hybridomas; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology*; D013950:Thymus Gland / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*; D000099259:N-myc Downstream-Regulated Gene 1 Protein
chemical_list: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; D018797:Cell Cycle Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D013196:Dihydrotestosterone; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; D013739:Testosterone; D000099259:N-myc Downstream-Regulated Gene 1 Protein