The photodynamic effect of Victoria blue BO on peripheral blood mononuclear and leukemic cells.
The photodynamic effect of Victoria blue BO (VB-BO) and photoirradiation on peripheral blood mononuclear cells was studied. The cells were preincubated with VB-BO followed by photoirradiation and overnight culture. The highest percentage of dead cells (propidium iodide assay in flow cytometry) was seen in the monocyte population. The lymphocytes showed a lower sensitivity to VB-BO photodynamic action than the monocytes (12% vs 80% of PI-positive cells). The effect of VB-BO and phototreatment on lymphocyte function was studied using a mitogen-induced proliferation assay. A decrease of mitogen response was observed. The VB-BO and photoirradiation were also used on leukemic cells. The leukemic cells from acute myeloid leukemia and B precursors leukemia were sensitive to VB-BO photodynamic action. The high VB-BO sensitivity of monocytes and leukemic cells (myeloid and lymphoid B derived) suggests possible application of VB-BO for selective depletion of monocytes or sensitive leukemic cells.
Fiedorowicz
M
M
Department of Chemistry, Hugo Kołłatay University of Agriculture, Krakow, Poland. rrfiedor@cyf-kr.edu.pl
Pituch-Noworolska
A
A
Zembala
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology; D001854:Bone Marrow Cells; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D007938:Leukemia / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000528:radiation effects; D009000:Monocytes / Q000187:drug effects / Q000528:radiation effects; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011744:Pyrimidinones / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D011836:Radiation Tolerance / Q000187:drug effects
D000970:Antineoplastic Agents; D000998:Antiviral Agents; D005456:Fluorescent Dyes; D017319:Photosensitizing Agents; D011744:Pyrimidinones; D000644:Quaternary Ammonium Compounds; C084160:Victoria blue BO; C003954:merocyanine dye