Stimulation of hepatocyte glycerolipid synthesis by iron/ADP is due to ADP rather than oxidative stress.
ADP-complexed Fe3+ has been used in various in vitro systems and in intact cells to induce lipid peroxidation. During studies on the effects of oxidative stress on lipid metabolism we observed a significant increase in de novo glycerolipid synthesis in Fe3+/ADP-treated rat hepatocytes as evidenced by increased [U-14C]glycerol incorporation. Here we show that this increase, largely due to enhanced triacylglycerol synthesis, is caused by ADP rather than Fe3+/ADP-induced oxidative stress. Hence, metabolic alterations due to treatment of intact cells by Fe3+/ADP must be interpreted with caution.
Girón-Calle
J
J
Hormel Institute, University of Minnesota, Austin 55912, USA.
Schmid
H H
HH
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005290:Ferric Compounds / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism*; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014280:Triglycerides / Q000378:metabolism
D005290:Ferric Compounds; D008055:Lipids; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D000244:Adenosine Diphosphate; D005990:Glycerol