Metabolism of endothelin-1 by neuroblastoma x glioma hybrid (NG108-15) cells.
The endothelin (ET) peptides, ET-1, ET-2, and ET-3, as well as the ETA and ETB receptor subtypes, are known to occur in brain, but there is a dearth of information on the metabolism of these peptides by the central nervous system (CNS). In this study we have investigated the kinetics of ET-1 binding to and dissociation from the hybrid neuroblastoma x glioma cell line, NG108-15, which is known to contain functional ET receptors, and metabolism of bound ET-1. [125I]ET-1 was incubated with cells for various periods of time up to 6 h, and the nature of the radioactivity in the cell medium and lysate was analyzed by reverse phase high performance liquid chromatography (HPLC). It was found that NG108-15 cells are capable of degrading [125I]ET-1 to [125I]Tyr and several fragments of intermediate hydrophobicity; however, a portion of the cell-associated [125I]ET-1 was protected from degradation for several hours.
Angelova
K
K
Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA.
Ergul
A
A
Peng
K C
KC
Puett
D
D
D002490:Central Nervous System / Q000378:metabolism* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D019332:Endothelin-1 / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism; D006822:Hybrid Cells / Q000378:metabolism*; D016014:Linear Models; D009447:Neuroblastoma / Q000378:metabolism; D011869:Radioligand Assay
D019332:Endothelin-1