Influenza-specific immunity induced by recombinant Listeria monocytogenes vaccines.
In this study, we evaluate two Listeria monocytogenes strains that express influenza nucleoprotein (NP) sequences for their ability to protect against challenge with influenza-virus. The construction of one strain, which expresses only the Kd restricted NP epitope (NP 147-155), is described in this study; the other strain, which expresses the full NP sequence in the form of a fusion protein, has been described previously. The ability of the two strains to present the Kd restricted NP epitope in vitro and induce NP-specific CTL in vivo is also described. Mice immunized by the intravenous route with either strain cleared a subsequent (3 weeks post-immunization) influenza virus infection more rapidly as indicated by reduced virus titers in the lungs 5 days after challenge. Efficacy of both recombinant L. monocytogenes strains as vaccines in this system was equivalent and equal to that of recombinant vaccinia expressing NP.
Ikonomidis
G
G
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Portnoy
D A
DA
Gerhard
W
W
Paterson
Y
Y
D000818:Animals; D002460:Cell Line; D000939:Epitopes / Q000378:metabolism; D007113:Immunity, Innate; D009980:Influenza A virus / Q000276:immunology* / Q000378:metabolism; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology*; D008168:Lung / Q000821:virology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control* / Q000628:therapy; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology / Q000378:metabolism; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000939:Epitopes; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014758:Viral Core Proteins; D014765:Viral Vaccines