Elastin-derived peptide induces monocyte chemotaxis by increasing intracellular cyclic GMP level and activating cyclic GMP dependent protein kinase.
An elastin-derived peptide with an average molecular mass of 25 kDa was shown to induce monocyte chemotaxis at the optimal concentration of 10(-1) micrograms/ml. Homologous deactivation test showed that monocytes exposed to the elastin-derived peptide at 10(-1) micrograms/ml lost their chemotactic responsiveness when reexposed to the same stimulus. In conjunction with chemotactic response to the elastin-derived peptide, intracellular guanosine 3', 5'-monophosphate (cGMP) levels were enhanced but intracellular adenosine 3', 5'-monophosphate (cAMP) levels were not. The monocyte migration induced by the elastin-derived peptide was inhibited by cGMP dependent protein kinase (PKG) inhibitor, but not by cAMP dependent protein kinase inhibitor and protein kinase C inhibitor. These results suggest that the elastin-derived peptide induces monocyte chemotaxis by increasing the level of cGMP, followed by activating PKG.
Uemura
Y
Y
Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Fukuoka, Japan.
Okamoto
K
K
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D053119:Benzophenanthridines; D002227:Carbazoles; D002417:Cattle; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D000242:Cyclic AMP / Q000737:chemistry; D006152:Cyclic GMP / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D017869:Cyclic GMP-Dependent Protein Kinases / Q000187:drug effects* / Q000378:metabolism; D004357:Drug Synergism; D004549:Elastin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007211:Indoles; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010617:Phenanthridines / Q000494:pharmacology; D013997:Time Factors
D000470:Alkaloids; D053119:Benzophenanthridines; D002227:Carbazoles; D007211:Indoles; D010617:Phenanthridines; C073601:KT 5823; D009240:N-Formylmethionine Leucyl-Phenylalanine; D004549:Elastin; D000242:Cyclic AMP; C016299:chelerythrine; D017869:Cyclic GMP-Dependent Protein Kinases; D006152:Cyclic GMP