Primary structure and functional analysis of the soluble transducer protein HtrXI in the archaeon Halobacterium salinarium.
Signal transduction in the archaeon Halobacterium salinarium is mediated by a family of 13 soluble and membrane-bound transducers. Here, we report the primary structure and functional analysis of one of the smallest halobacterial putative transducers, HtrXI. Hydropathy plot analysis of the primary structure predicts no membrane-spanning segments in HtrXI. The fractionation of the H. salinarium proteins confirmed that HtrXI is a soluble protein. Capillary assay with an HtrXI deletion mutant and a complemented strain revealed that this soluble transducer is involved in Asp and Glu taxis. In vivo analysis of the methylesterase activity of the htrXI-1 deletion mutant suggests that HtrXI plays an important role in the adaptation of the chemotactic responses to His, Asp, and Glu, which are attractants for halobacteria. Stimulation by Asp and Glu causes demethylation of HtrXI and of another putative transducer, HtrVII. But addition of His to halobacterial cells increases HtrXI methylation together with that of other putative transducers. In the absence of HtrXI, stimulation by either Glu or His does not decrease or increase the methylation of any putative transducers. Therefire, the HtrXI transducer appears to have a complex role in chemotaxis signal transduction.
Brooun
A
A
Department of Microbiology, University of Hawaii, Honolulu.
Zhang
W
W
Alam
M
M
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D001224:Aspartic Acid; D002633:Chemotaxis; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D018698:Glutamic Acid; D006217:Halobacterium / Q000235:genetics / Q000502:physiology*; D006639:Histidine; D008780:Methyltransferases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017384:Sequence Deletion; D015398:Signal Transduction; D012995:Solubility; D016335:Zinc Fingers
D019843:Archaeal Proteins; C121347:HtrXI protein, Halobacterium salinarum; D011994:Recombinant Proteins; D001224:Aspartic Acid; D018698:Glutamic Acid; D006639:Histidine; D008780:Methyltransferases