Detection of mosquito saliva-specific IgE antibodies by capture ELISA.
We developed an IgE-capture ELISA and measured mosquito saliva-specific IgE antibodies in 27 children sensitive to mosquito bites. Children with large 15-min bite wheals had significantly higher (P < 0.0005) mosquito saliva-specific IgE levels than children with small wheals. In the latter group, the saliva-specific IgE level was significantly higher (P = 0.031) than the levels of six infants never exposed to mosquitoes. A positive correlation (r = 0.65; P = 0.0002) was found between the size of the 15-min wheal and the mosquito saliva-specific IgE antibody levels. These results further support the role of mosquito saliva-specific IgE antibodies in the pathogenesis of mosquito-bite whealing. Compared to immunoblotting, IgE-capture ELISA provides a quantitative method to measure mosquito saliva-specific IgE antibodies.
Brummer-Korvenkontio
H
H
National Public Health Institute, Helsinki, Finland.
Palosuo
K
K
Palosuo
T
T
Brummer-Korvenkontio
M
M
Leinikki
P
P
Reunala
T
T
D000818:Animals; D002648:Child; D002675:Child, Preschool; D009033:Culicidae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D007223:Infant; D012463:Saliva / Q000276:immunology*
D007073:Immunoglobulin E