Palladium or platinum exacerbates hydroxyl radical mediated DNA damage.
Strand breakage of supercoiled pBR322 DNA by a Fenton system is increased in the presence of palladium or platinum (Pt) ions. Neither Pd nor Pt ions can substitute for iron in the Fenton system. We have obtained several lines of evidence that Pd and Pt ions in the presence of a Fenton system can augment the production of OH., as monitored by a spectrophotometric method quantifying hydroxylated salicylate or by a fluorometric method quantifying catechol production. Furthermore, the promoting effect of both metal ions on OH. production was substantiated by the identification of multiple hydroxylated products of salicylate [2,3-dihydroxybenzoate (A), 2,5-dihydroxybenzoate (B), and catechol (C)] using HPLC. The concentrations of A, B, and C produced in the control were 4.5, 8.0, and 2.0 microM, respectively; whereas, their respective concentrations increased to 23.6, 42.0 and 10.0 microM with the addition of Pd ions. The observed phenomenon was further confirmed by the identification of HO-DMPO spin adducts using ESR spectroscopy. Taken together, our data suggest that the mechanism of Pd or Pt ion-mediated exacerbation of DNA damage by a Fenton system is due to the promotion of OH. production by these metal ions.
Liu
T Z
TZ
School of Medical Technology, Chang Gung College of Medicine and Technology, Taoyuan Taiwan.
Lin
T F
TF
Chiu
D T
DT
Tsai
K J
KJ
Stern
A
A
D004249:DNA Damage; D004278:DNA, Superhelical / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004587:Electrophoresis, Agar Gel; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000378:metabolism*; D006900:Hydroxylation; D007501:Iron / Q000378:metabolism / Q000494:pharmacology; D010165:Palladium / Q000494:pharmacology*; D010957:Plasmids; D010984:Platinum / Q000494:pharmacology*; D012459:Salicylates / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D004278:DNA, Superhelical; C045076:Fenton's reagent; D005609:Free Radicals; D012459:Salicylates; D017665:Hydroxyl Radical; D010984:Platinum; D010165:Palladium; D006861:Hydrogen Peroxide; D007501:Iron