Effects of low-energy gallium-aluminum-arsenide laser irradiation on cultured fibroblasts and keratinocytes.
OBJECTIVE
To assess whether the gallium-aluminum-arsenide low energy laser will increase cell proliferation, cell attachment, or cell migration in cultured fibroblasts and keratinocyte models.


METHODS
Monolayer cultures of fibroblasts and keratinocytes were subjected to gallium-aluminum-arsenide laser irradiation at varying power densities for varying time intervals. Cell proliferation was assessed by absorbent spectrophotometry while cell adhesion was assessed by a microcolorimetric assay for cells attached to bovine dermis collagen. Cell migration was assessed through a filter utilizing high power microscopic fields.


RESULTS
There were no differences in cell proliferation, adhesion, or migration in either the fibroblasts or keratinocyte culture treated with the gallium-aluminum-arsenide laser at any power density or time compared with nontreated controls.


CONCLUSIONS
The gallium-aluminum-arsenide laser, when utilized at powers 5-100 milliwatts and times of between 10-120 seconds has no biostimulatory effects on fibroblasts or keratinocyte cultures as assessed by cell proliferation, adhesion, or migration.
Pogrel
M A
MA
Department of Oral Surgery, School of Dentistry, University of California, San Francisco 94143-0440, USA.
Chen
J W
JW
Zhang
K
K
D000535:Aluminum; D001152:Arsenicals; D002452:Cell Count; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000528:radiation effects*; D005708:Gallium; D006801:Humans; D015603:Keratinocytes / Q000528:radiation effects*; D007834:Lasers
D001152:Arsenicals; C043055:gallium arsenide; D005708:Gallium; D000535:Aluminum