title: Human 5-HT5A receptor gene: systematic screening for DNA sequence variation and linkage mapping on chromosome 7q34-q36 using a polymorphism in the 5' untranslated region.
abstract: Serotonin (5-hydroxytryptamine, 5-HT) is a neurotransmitter that mediates a wide range of sensory, motor, and cortical functions by activating multiple 5-HT receptor subtypes. In the present study we performed a systematic mutation scan of the complete coding region of the 5-HT5A receptor to explore its variability in the general population. Investigating 46 unrelated healthy subjects by single-strand conformation analysis no sequence changes of likely functional relevance were observed. The detection of a frequent G-->C substitution at position -19 was used for fine scale linkage mapping of the 5-HT5A gene. Employing a polymerase-chain-reaction based assay we genotyped 7 CEPH families (Centre d'Etude du Polymorphisme Humaine) and mapped the receptor to genetic markers on chromosome 7q34-q36.
authors.affiliation: Institute of Human Genetics, University of Bonn, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Shimron-Abarbanell
authors.forename: J
authors.initials: J
authors.lastname: Erdmann
authors.forename: I R
authors.initials: IR
authors.lastname: Vogt
authors.forename: S P
authors.initials: SP
authors.lastname: Bryant
authors.forename: N K
authors.initials: NK
authors.lastname: Spurr
authors.forename: M
authors.initials: M
authors.lastname: Knapp
authors.forename: P
authors.initials: P
authors.lastname: Propping
authors.forename: M M
authors.initials: MM
authors.lastname: Nöthen
mesh_terms: D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D008040:Genetic Linkage; D014644:Genetic Variation; D006801:Humans; D011110:Polymorphism, Genetic; D011985:Receptors, Serotonin / Q000235:genetics*
chemical_list: D011985:Receptors, Serotonin; C078730:serotonin 5 receptor