[The genetic transformation of Lycopersicon peruvianum var. dentatum by using Agrobacterium tumefaciens carrying a plasmid with the human beta-interferon gene].
Wild tomato plants of Lycopersicon peruvianum var. dentatum were transformed with pG11 plasmid having human beta-interferon (hIFN-beta) and NPTII genes. Transformation was demonstrated by polymerase chain reaction (PCR) and ELISA assay. Expression of hIFN-beta gene was identified by Western blot analysis. Transgenic plants produced seeds and F1 generation inherited integrated traits.
Rudas
V A
VA
Piven'
N M
NM
Rivkin
M I
MI
Vershinin
A V
AV
Infante
D C
DC
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors; D001483:Base Sequence; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D006801:Humans; D016899:Interferon-beta / Q000235:genetics*; D007612:Kanamycin / Q000037:antagonists & inhibitors; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014170:Transformation, Genetic / Q000235:genetics*
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D007612:Kanamycin; D016899:Interferon-beta
Geneticheskaia transformatsiia Lycopersicon peruvianum var. dentatum s pomoshch'iu Agrobacterium tumefaciens, nesushcheĭ plazmidu s genom beta-interferona cheloveka.