title: A general method for DNA polymorphism identification in genetic assessment and molecular diagnosis.
abstract: A general methodology for the analysis of human diseases at the nucleic acid level has been described. This method consists of a semiautomated, nontoxic analytic procedure, staining DNA bands with silver nitrate instead of ethidium bromide after DNA amplification by PCR. This technique provides a sensitive assay with enhanced accuracy and offers the possibility of carrying out the molecular analysis of several polymorphisms in a single day simplifying molecular genetics with diagnostic purposes.
authors.affiliation: EuroEspes Biomedical Research Center, Department of Clinical and Molecular Genetics, Molecular Neuropharmacology and Neurogeriatrics, La Coruña, Spain.
authors.forename: K
authors.initials: K
authors.lastname: Beyer
authors.forename: J I
authors.initials: JI
authors.lastname: Lao
authors.forename: X A
authors.initials: XA
authors.lastname: Alvarez
authors.forename: R
authors.initials: R
authors.lastname: Cacabelos
mesh_terms: D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D030342:Genetic Diseases, Inborn / Q000175:diagnosis*; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012835:Silver Nitrate
chemical_list: D004247:DNA; D012835:Silver Nitrate