HPLC determination of ITF 188 and its metabolite ITF 1078 in urine after intranasal administration of new heparin salt ITF 1300 to dogs.
Heparin salt ITF 1300 in which low molecular weight heparin is salified with a new counterion, di-[3-(N,N-dibutylamino)]propyl carbonate (ITF 188), was selected for the pharmacological development. A specific, sensitive and reproducible HPLC method for the determination of ITF 188 and its alcoholic metabolite ITF 1078 in urine was developed. The method was employed for the study of urinary excretion of the counterion after intranasal administration of ITF 1300 to dogs. The total amount of ITF 188 and ITF 1078 found in urine within 72 hours after the nasal instillation of ITF 1300 accounts for about 5% of the administered dose.
Monzani
M V
MV
Italfarmaco Research Center, Cinisello B. Milan.
Coltro
G
G
Sala
A
A
D000281:Administration, Intranasal; D000818:Animals; D002082:Butylamines / Q000008:administration & dosage / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005260:Female; D005343:Fibrinolytic Agents / Q000008:administration & dosage / Q000652:urine*; D006493:Heparin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000652:urine; D008297:Male
C106341:3-(N,N-dibutylamino)-1-propanol; D002082:Butylamines; D005343:Fibrinolytic Agents; C106340:di-(3-(N,N-dibutylamino))propyl carbonate; C078918:ITF 300; D006493:Heparin