title: Effects of low-energy gallium-aluminum-arsenide laser irradiation on cultured fibroblasts and keratinocytes.
abstract: OBJECTIVE
  To assess whether the gallium-aluminum-arsenide low energy laser will increase cell proliferation, cell attachment, or cell migration in cultured fibroblasts and keratinocyte models.
  
  
  METHODS
  Monolayer cultures of fibroblasts and keratinocytes were subjected to gallium-aluminum-arsenide laser irradiation at varying power densities for varying time intervals. Cell proliferation was assessed by absorbent spectrophotometry while cell adhesion was assessed by a microcolorimetric assay for cells attached to bovine dermis collagen. Cell migration was assessed through a filter utilizing high power microscopic fields.
  
  
  RESULTS
  There were no differences in cell proliferation, adhesion, or migration in either the fibroblasts or keratinocyte culture treated with the gallium-aluminum-arsenide laser at any power density or time compared with nontreated controls.
  
  
  CONCLUSIONS
  The gallium-aluminum-arsenide laser, when utilized at powers 5-100 milliwatts and times of between 10-120 seconds has no biostimulatory effects on fibroblasts or keratinocyte cultures as assessed by cell proliferation, adhesion, or migration.
authors.affiliation: Department of Oral Surgery, School of Dentistry, University of California, San Francisco 94143-0440, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Pogrel
authors.forename: J W
authors.initials: JW
authors.lastname: Chen
authors.forename: K
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000535:Aluminum; D001152:Arsenicals; D002452:Cell Count; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000528:radiation effects*; D005708:Gallium; D006801:Humans; D015603:Keratinocytes / Q000528:radiation effects*; D007834:Lasers
chemical_list: D001152:Arsenicals; C043055:gallium arsenide; D005708:Gallium; D000535:Aluminum