title: 1-(5-phospho-beta-D-ribosyl)2'-phosphoadenosine 5'-phosphate cyclic anhydride induced Ca2+ release in human T-cell lines.
abstract: 1-(5-Phospho-beta-D-ribosyl)2'-phosphoadenosine 5'-phosphate cyclic anhydride [2'-phospho-cyclic ADP-ribose, cAdo(2')P(5')PP-Rib] was prepared enzymatically from NADP+ using ADP-ribosyl-cyclase from Aplysia californica. The product was purified by HPLC and characterized by NMR and mass spectroscopy, by conversion to 1-(5-phospho-beta-D-ribosyl)adenosine 5'-phosphate cyclic anhydride (cADP-Rib) by alkaline phosphatase and by resistance to snake venom phosphodiesterase. cAdo-(2')P(5')PP-Rib dose-dependently released Ca2+ from an intracellular, non-endoplasmic reticular Ca2+ pool of permeabilized Jurkat and HPB. ALL T-lymphocytes. In contrast, the closely related compounds 1-(5-phospho-beta-D-ribosyl)3'phosphoadenosine 5'-phosphate cyclic anhydride and 1-(5-phospho-beta-D-ribosyl)cyclic 2',3'-phosphoadenosine 5'-phosphate cyclic anhydride did not induce Ca2+-release from permeabilized T cells. The Ca2+ pool sensitive to cAdo(2')P(5')PP-Rib partially overlapped with the Ca2+ pool sensitive to cADP-Rib recently described in T cells [Guse, A. H., da Silva, C. P., Emmrich, F., Ashamu, G. A., Potter, B. V. L. & Mayr, G. W. (1995) Characterization of cyclic adenosine diphosphate-ribose-induced Ca2+-release in T-lymphocyte cell lines, J. Immunol. 155, 3353-3359]. Control experiments suggest that the results were neither due to Ca2+ contaminations in the cADP-Rib preparation nor to catabolism of cAdo(2')P(5')PP-Rib to cADP-Rib.
authors.affiliation: Institut für Physiologische Chemie, Abteilung für Enzymchemie, Universität Hamburg, Germany. guse@uke.uni-hamburg.de
authors.forename: A H
authors.initials: AH
authors.lastname: Guse
authors.forename: C P
authors.initials: CP
authors.lastname: da Silva
authors.forename: K
authors.initials: K
authors.lastname: Weber
authors.forename: C N
authors.initials: CN
authors.lastname: Armah
authors.forename: G A
authors.initials: GA
authors.lastname: Ashamu
authors.forename: C
authors.initials: C
authors.lastname: Schulze
authors.forename: B V
authors.initials: BV
authors.lastname: Potter
authors.forename: G W
authors.initials: GW
authors.lastname: Mayr
authors.forename: H
authors.initials: H
authors.lastname: Hilz
mesh_terms: D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000378:metabolism; D001048:Aplysia; D002118:Calcium / Q000378:metabolism*; D002451:Cell Compartmentation; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000494:pharmacology; D036563:Cyclic ADP-Ribose; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D019169:Jurkat Cells; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009249:NADP / Q000031:analogs & derivatives*; D012430:Ruthenium Red / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C106363:1-(5-phosphoribosyl)-2'-phosphoadenosine 5'-phosphate cyclic anhydride; D015703:Antigens, CD; D000943:Antigens, Differentiation; D004396:Coloring Agents; D008562:Membrane Glycoproteins; D012430:Ruthenium Red; D036563:Cyclic ADP-Ribose; D000246:Adenosine Diphosphate Ribose; D009249:NADP; D000242:Cyclic AMP; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1; D002118:Calcium