Adriamycin binds to the matrix of secretory granules during mast cell exocytosis.
The antineoplastic drug adriamycin induces exocytosis in rat peritoneal mast cells followed by a significant uptake of the drug into the secretory granules. The drug is fluorescent, allowing visualization of its accumulation and binding to mast cell granules by fluorescence microscopy. At the same time, the well known inorganic dye ruthenium red was used as a probe because of its great affinity for heparin in the mast cell secretory granules as visualized by bright field microscopy. Competition between adriamycin and ruthenium red for binding to the negatively charged matrix of granules was demonstrated. Biochemical studies were also performed to confirm microscopic observations. Adriamycin may be of interest for studying mast cell secretion; it is not only a strong fluorescent dye for mast cell granules that are in communication with the extracellular space, but it also induces mast cell exocytosis.
Crivellato
E
E
Department of Medical and Morphological Research, University of Udine, Italy.
Candussioi
L
L
Decorti
G
G
Klugmann
F B
FB
Baldini
L
L
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D004396:Coloring Agents; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000648:ultrastructure; D004317:Doxorubicin / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D005089:Exocytosis / Q000502:physiology*; D008407:Mast Cells / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012430:Ruthenium Red / Q000378:metabolism*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000903:Antibiotics, Antineoplastic; D004396:Coloring Agents; D012430:Ruthenium Red; D003189:p-Methoxy-N-methylphenethylamine; D004317:Doxorubicin