Perfluoro-15-crown-5-ether labelled macrophages in adoptive transfer experimental allergic encephalomyelitis.
In this serial in vivo study, macrophages labelled with perfluoro-15-crown-5-ether (15C5) were monitored in rats after inducing adoptive transfer experimental allergic encephalomyelitis (AT-EAE). AT-EAE is an animal model of multiple sclerosis and is characterized by inflammatory infiltrates in the central nervous system (CNS) and breakdown of the blood-brain-barrier. A particular feature of AT-EAE are macrophage infiltrates. Purpose of this study was to monitor the invasive and evasive phase of the macrophages in AT-EAE by using 3-dimensional 19F magnetic resonance imaging (3D 19F-MRI). In the early stage of the disease, a much stronger 19F-signal intensity was observed in AT-EAE-rats than in healthy control rats in the tissue adjacent to CNS regions severely affected by inflammatory infiltrates, and thereafter the 19F-signal intensity was decreasing over the time. However, no 19F-signal could be observed in the CNS itself neither in AT-EAE-rats nor in control rats. According to these findings it is assumed that we monitored the evasion of the macrophages from the region of inflammation.
Nöth
U
U
Physikalisches Institut, Biophysik, Am Hubland, Würzburg, Germany.
Morrissey
S P
SP
Deichmann
R
R
Jung
S
S
Adolf
H
H
Haase
A
A
Lutz
J
J
D000818:Animals; D043844:Crown Ethers; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D004988:Ethers, Cyclic; D005260:Female; D007275:Injections, Intravenous; D008264:Macrophages / Q000276:immunology*; D008279:Magnetic Resonance Imaging; D008526:Medulla Oblongata / Q000166:cytology / Q000276:immunology; D008636:Mesencephalon / Q000166:cytology / Q000276:immunology; D009103:Multiple Sclerosis / Q000175:diagnosis / Q000276:immunology / Q000473:pathology; D011149:Pons / Q000166:cytology / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000166:cytology / Q000276:immunology; D015431:Weight Loss; D005466:Fluorocarbons
D043844:Crown Ethers; D004988:Ethers, Cyclic; C089317:perfluoro-15-crown-5-ether; D005466:Fluorocarbons