Extracellular DNA from Serpulina hyodysenteriae consists of 6.5 kbp random fragments of chromosomal DNA.
Preparations of chromosomal DNA from a number of Serpulina hyodysenteriae strains have shown, using agarose gel electrophoresis, the presence of an additional band with a mobility similar to that of a 6.5 kbp linear DNA fragment. Analysis showed that this is not a plasmid but rather a form of extracellular DNA like that observed for Gram-negative bacteria. However, unlike the extracellular DNA from Gram-negative bacteria, which showed a similar band profile to that of the DNA from whole cells, that from S. hyodysenteriae consisted primarily of fragments of a fixed 6.5 kbp.
Turner
A K
AK
BBSRC Institute for Animal Health, Compton, Newbury, Berks, UK. keith turner@bbsrc.ac.uk
Sellwood
R
R
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D017401:Brachyspira hyodysenteriae / Q000737:chemistry*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D003850:Deoxyribonuclease I; D008074:Lipoproteins; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D014169:Transformation, Bacterial
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D008074:Lipoproteins; C095510:SmpA protein, Treponema hyodysenteriae; D003850:Deoxyribonuclease I