Microplate hybridization for Borna disease virus RNA in human peripheral blood mononuclear cells.
We developed a simple and sensitive microplate hybridization procedure with which to identify Borna disease virus cDNA in amplified products from human peripheral blood mononuclear cells. The mean values for the positive PCR products were significant compared with those for any of the negative products, indicating that this method can be applied to rapidly diagnose a large number of clinical specimens.
Fujiwara
S
S
Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo, Japan.
Takahashi
H
H
Nakaya
T
T
Nakamura
Y
Y
Nakamura
K
K
Iwahashi
K
K
Kazamatsuri
H
H
Iritani
S
S
Kuroki
N
N
Ikeda
K
K
Ikuta
K
K
D000818:Animals; D001483:Base Sequence; D001782:Blood Donors; D001890:Borna Disease / Q000175:diagnosis / Q000821:virology; D001891:Borna disease virus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D017931:DNA Primers; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D001523:Mental Disorders / Q000209:etiology / Q000821:virology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000235:genetics
D017931:DNA Primers; D004279:DNA, Viral; D014764:Viral Proteins; C084757:p24 protein, Borna disease virus