title: Sensitivity of ATPase-ADPase activities from synaptic plasma membranes of rat forebrain to lipid peroxidation in vitro and the protective effect of vitamin E.
abstract: The in vitro effects of membrane lipid peroxidation on ATPase-ADPase activities in synaptic plasma membranes from rat forebrain were investigated. Treatment of synaptic plasma membranes with an oxidant generating system (H(2)0(2)/Fe(2+)/ascorbate) resulted in lipid peroxidation and inhibition of the enzyme activity. Besides, trolox as a water soluble vitamin E analogue totally prevented lipid peroxidation and the inhibition of enzyme activity. These results demonstrate the susceptibility of ATPase-ADPase activities of synaptic plasma membranes to free radicals and suggest that the protective effect against lipid peroxidation by trolox prevents the inhibition of enzyme activity. Thus, inhibition of ATPase-ADPase activities of synaptic plasma membranes in cerebral oxidative stress probably is related to lipid peroxidation in the brain.
authors.affiliation: Departamento de Bioquimica, Instituto de Biociencias, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Vietta
authors.forename: S S
authors.initials: SS
authors.lastname: Frassetto
authors.forename: A M
authors.initials: AM
authors.lastname: Battastini
authors.forename: A
authors.initials: A
authors.lastname: Bello-Klein
authors.forename: C
authors.initials: C
authors.lastname: Moreira
authors.forename: R D
authors.initials: RD
authors.lastname: Dias
authors.forename: J J
authors.initials: JJ
authors.lastname: Sarkis
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001081:Apyrase / Q000378:metabolism*; D001205:Ascorbic Acid / Q000494:pharmacology; D002839:Chromans / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D015227:Lipid Peroxidation; D008297:Male; D016548:Prosencephalon / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013570:Synaptic Membranes / Q000201:enzymology*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002839:Chromans; D005296:Ferrous Compounds; C051140:iron(II)-ascorbic acid complex; D014810:Vitamin E; D006861:Hydrogen Peroxide; D000251:Adenosine Triphosphatases; D001081:Apyrase; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; C029451:ferrous chloride