Expression of the acatalytic carbonic anhydrase VIII gene, Car8, during mouse embryonic development.
The carbonic anhydrase (CA)-like protein, CA VIII, lacks the typical carbon dioxide hydrase activity of the CA isozymes. However, the high degree of amino acid sequence similarity between the products of the mouse and the human CA VIII genes suggests an important biological function. We have attempted to investigate the function of this gene in mammalian development by conducting an in situ hybridization study on sagittal sections of mouse embryos at gestation days of 9.5-16.5 using a 35S-labelled riboprobe. Results indicate that this gene (called Car8 in mice) is expressed as early as day 9.5 in a variety of organs including liver, branchial arches, neuroepithelium and developing myocardium. Between days 10.5 and 12.5, it showed a widespread distribution of mRNA expression that became more restricted as development progressed. The level of expression of Car8 mRNA was relatively high in the brain, liver, lung, heart, gut, thymus and epithelium covering the head and the oronasal cavity.
Lakkis
M M
MM
Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109, USA.
Bergenhem
N C
NC
O'Shea
K S
KS
Tashian
R E
RE
D000818:Animals; D014408:Biomarkers, Tumor; D002256:Carbonic Anhydrases / Q000235:genetics; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D014018:Tissue Distribution; D047908:Intracellular Signaling Peptides and Proteins
D014408:Biomarkers, Tumor; D002256:Carbonic Anhydrases; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C512821:Car8 protein, mouse; C512818:CA8 protein, human; D047908:Intracellular Signaling Peptides and Proteins