title: Determination of organophosphorous pesticides in biological samples of acute poisoning by HPLC with diode-array detector.
abstract: We have developed a simple and rapid method for measuring 11 organophosphorous pesticides (dichlorvos, methidathion, salithion, malathion, fenitrothion, fenthion, parathion, diazinon, ethylthiometon, O-ethyl O-(4-nitrophenyl)phenylphosphonothioate (EPN) and chlorpyrifos) and one metabolite (3-methyl-4-nitrophenol) of fenitrothion in serum and urine of acute poisoning patients by HPLC with a diode-array detector. An aliquot of the biological sample after deproteinization by acetonitrile was injected into C18 column using acetonitrile-water as a mobile phase. The detection limits in serum and urine ranged from 0.05 to 6.8 micrograms/ml at a wavelength of 230 nm. This method was successfully applied to two actual cases of acute poisoning.
authors.affiliation: Department of Pharmacy, Yamaguchi University Hospital, Ube, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Cho
authors.forename: N
authors.initials: N
authors.lastname: Matsuoka
authors.forename: A
authors.initials: A
authors.lastname: Kamiya
mesh_terms: D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005278:Fenitrothion / Q000097:blood / Q000506:poisoning / Q000652:urine; D006801:Humans; D007306:Insecticides / Q000097:blood / Q000506:poisoning* / Q000652:urine; D008297:Male; D008875:Middle Aged
chemical_list: D007306:Insecticides; D005278:Fenitrothion