Inhibition by 2-arachidonoylglycerol, a novel type of possible neuromodulator, of the depolarization-induced increase in intracellular free calcium in neuroblastoma x glioma hybrid NG108-15 cells.
2-Arachidonoylglycerol was found to inhibit the depolarization-induced increase in [Ca2+]i in NG108-15 cells differentiated with prostaglandin E1 and theophylline in a dose-dependent manner. Such an effect appears to be rather specific to polyunsaturated fatty acid-containing monoacylglycerols such as 2-arachidonoylglycerol. Neither 2-palmitoylglycerol nor free arachidonic acid exhibited appreciable inhibitory activity. These observations raise the possibility that 2-arachidonoylglycerol attenuates the increase in [Ca2+]i, thereby modulating several neural functions in this type of cell.
Sugiura
T
T
Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa, Japan.
Kodaka
T
T
Kondo
S
S
Tonegawa
T
T
Nakane
S
S
Kishimoto
S
S
Yamashita
A
A
Waku
K
K
D001095:Arachidonic Acids; D002118:Calcium / Q000378:metabolism*; D063388:Endocannabinoids; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D005989:Glycerides / Q000494:pharmacology*; D006822:Hybrid Cells; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001095:Arachidonic Acids; D063388:Endocannabinoids; D005989:Glycerides; D018377:Neurotransmitter Agents; C094503:glyceryl 2-arachidonate; D002118:Calcium