title: Molecular forms of somatostatin-like immunoreactivity in the cerebrospinal fluid of patients with senile dementia of the Alzheimer type.
abstract: In the cerebrospinal fluid (CSF) of 53 patients with senile dementia of the Alzheimer type (SDAT) and 12 elderly controls, we measured somatostatin (SLI) and its molecular forms: high-molecular weight form (HMV-SST), somatostatin-14 (SST-14), somatostatin-25/28 (SST-28/25), and des-ala-somatostatin (des-ala-SST) using high pressure liquid chromatography (HPLC) and a radioimmunoassay. In SDAT, SLI was significantly decreased (p < 0.05) and correlated with dementia scores (r = -0.65, p < 0.05). HPLC separation showed a marked heterogeneity of SLI in the CSF with a preponderance of SST-14 and SST-25/28. The significant loss of SST-14 (p < 0.05) in SDAT was found to be correlated with dementia scores (r = 0.65). Moreover, qualitative and quantitative changes in the molecular pattern of SLI in SDAT indicated dysregulated synthesis and/or processing of somatostatin relating to the severity of dementia. The long-term administration of neuroleptics in severe cases of SDAT caused a significant increase of SLI (p < 0.05) and influenced the ratio of HMV-SST/SST-14 and SST25/28/SST-14.
authors.affiliation: Department of Neurology, University of Saarland, Homburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Strittmatter
authors.forename: H
authors.initials: H
authors.lastname: Cramer
authors.forename: C
authors.initials: C
authors.lastname: Reuner
authors.forename: D
authors.initials: D
authors.lastname: Strubel
authors.forename: G
authors.initials: G
authors.lastname: Hamann
authors.forename: K
authors.initials: K
authors.lastname: Schimrigk
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000188:drug therapy; D014150:Antipsychotic Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D002746:Chlorpromazine / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D003072:Cognition Disorders / Q000175:diagnosis; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008297:Male; D008970:Molecular Weight; D013004:Somatostatin / Q000096:biosynthesis / Q000134:cerebrospinal fluid*
chemical_list: D014150:Antipsychotic Agents; D013004:Somatostatin; D002746:Chlorpromazine