HIV-1 recombinant gp41 induces IL-10 expression and production in peripheral blood monocytes but not in T-lymphocytes.
The effects of recombinant gp41 (rgp41) protein of the human immunodeficiency virus type 1 (HIV-1) on interleukin 10 (IL-10) expression and production using human peripheral blood mononuclear cells was investigated. Expression of IL-10 mRNA was demonstrated within 3 h of cell exposure to endotoxin-free rgp41 by RT-PCR and Northern blot analyses in a time- and dose-dependent manner. IL-10 protein was detected in the supernatants of peripheral blood mononuclear cells following stimulation with rgp41 also in a dose dependent manner. Fractionation of peripheral blood mononuclear cells showed that purified monocytes but not purified T-lymphocytes induced expression of IL-10 mRNA by rgp41. Recombinant HIV-1 gp120 exhibits similar influences on the induction of IL-10. These results indicate that both of these components of envelope proteins may play an important role in HIV related immunomodulation by influencing regulatory functions of monocytes and macrophages.
Koutsonikolis
A
A
All Children's Hospital, Department of Pediatrics, University of South Florida, St. Petersburg 33701, USA.
Haraguchi
S
S
Brigino
E N
EN
Owens
U E
UE
Good
R A
RA
Day
N K
NK
D005591:Chemical Fractionation; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D015699:HIV Envelope Protein gp120 / Q000276:immunology / Q000494:pharmacology; D015700:HIV Envelope Protein gp41 / Q000276:immunology* / Q000494:pharmacology; D015497:HIV-1 / Q000737:chemistry; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016753:Interleukin-10