Interleukin-13 but not interleukin-4 prolongs eosinophil survival and induces eosinophil chemotaxis.
The effects of recombinant human (rh) interleukin (IL)-4 or rhIL-13 on survival, and chemotactic activity of human eosinophils were examined. Only rhIL-13 prolonged eosinophil survival in a dose-dependent manner above 3 ng/ml. Eosinophil survival induced by rhIL-13 was inhibited by monoclonal antibodies (mAbs) against IL-3 (p < 0.01) and granulocyte-macrophage colony-stimulating factor (GM-CSF) (p < 0.05), suggesting that rhIL-13 induced IL-3 and GM-CSF production from eosinophils and an autocrine mechanism is responsible for the eosinophil survival. The effects of rhIL-13 on eosinophil chemotactic activity were also examined. rhIL-13 showed chemotactic activity for eosinophils in a dose-dependent manner. Checkerboard analysis revealed that eosinophil migration was dependent on the concentration gradient, confirming that rhIL-13 is a chemotactic factor. rhIL-4 showed no effects. IL-13 may play an important role in the survival and recruitment of eosinophils in allergic diseases.
Horie
S
S
First Department of Internal Medicine, Shinshu University School of Medicine, Nagano.
Okubo
Y
Y
Hossain
M
M
Sato
E
E
Nomura
H
H
Koyama
S
S
Suzuki
J
J
Isobe
M
M
Sekiguchi
M
M
D015550:Cell Degranulation / Q000187:drug effects; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004306:Dose-Response Relationship, Immunologic; D004804:Eosinophils / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D018793:Interleukin-13 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000096:biosynthesis; D015847:Interleukin-4; D011994:Recombinant Proteins / Q000494:pharmacology
D018793:Interleukin-13; D007377:Interleukin-3; D011994:Recombinant Proteins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor