Two domains within sigmaN (sigma54) cooperate for DNA binding.
The sigma-N (sigmaN) subunit of the bacterial RNA polymerase is a sequence specific DNA-binding protein. The RNA polymerase holoenzyme formed with sigmaN binds to promoters in an inactive form and only initiates transcription when activated by enhancer-binding positive control proteins. We now provide evidence to show that the DNA-binding activity of sigmaN involves two distinct domains: a C-terminal DNA-binding domain that directly contacts DNA and an adjacent domain that enhances DNA-binding activity. The sequences required for the enhancement of DNA binding can be separated from the sequences required for core RNA polymerase binding. These results provide strong evidence for communication between domains within a transcription factor, likely to be important for the function of sigmaN in enhancer-dependent transcription.
Cannon
W V
WV
Department of Biology, Imperial College of Science, Technology and Medicine, London, SW7 2BB, United Kingdom.
Chaney
M K
MK
Wang
X
X
Buck
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009591:Nitrogenase / Q000235:genetics; D010088:Oxidoreductases; D010446:Peptide Fragments / Q000737:chemistry; D011401:Promoter Regions, Genetic; D051877:RNA Polymerase Sigma 54; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012808:Sigma Factor / Q000737:chemistry* / Q000378:metabolism*; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000502:physiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C031540:M-protein, influenza virus; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011994:Recombinant Proteins; D012808:Sigma Factor; D014763:Viral Matrix Proteins; D010088:Oxidoreductases; D009591:Nitrogenase; C029130:nitrogenase reductase; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54