Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress.
Exposure of Arabidopsis plants that were maintained under low light (200 mumol of photons m-2 sec-1) to excess light (2000 mumol of photons m-2 sec-1) for 1 hr caused reversible photoinhibition of photosynthesis. Measurements of photosynthetic parameters and the use of electron transport inhibitors indicated that a novel signal transduction pathway was initiated at plastoquinone and regulated, at least in part, by the redox status of the plastoquinone pool. This signal, which preceded the photooxidative burst of hydrogen peroxide (H2O2) associated with photoinhibition of photosynthesis, resulted in a rapid increase (within 15 min) in mRNA levels of two cytosolic ascorbate peroxidase genes (APX1 and APX2). Treatment of leaves with exogenous reduced glutathione abolished this signal, suggesting that glutathione or the redox status of the glutathione pool has a regulatory impact on this signaling pathway. During recovery from photooxidative stress, transcripts for cytosolic glutathione reductase (GOR2) increased, emphasizing the role of glutathione in this stress.
Karpinski
S
S
Department of Applied Genetics, John Innes Centre, Norwich Research Park, Colney, United Kingdom. stanislaw.karpinski@genfys.slu.se
Escobar
C
C
Karpinska
B
B
Creissen
G
G
Mullineaux
P M
PM
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins; D060387:Ascorbate Peroxidases; D018076:DNA, Complementary; D004579:Electron Transport; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D019803:Glutathione Disulfide; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D010788:Photosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D006861:Hydrogen Peroxide; D010544:Peroxidases; C555293:APX2 protein, Arabidopsis; D060387:Ascorbate Peroxidases; D005978:Glutathione; D019803:Glutathione Disulfide