Regulation of human renin secretion and gene transcription in Calu-6 cells.
Calu-6 cells were characterized for studying the transcriptional regulation of the human renin gene. Analysis of cis-acting elements of the renin promoter showed the highest activity within the first 582 bp in serum-free conditions and of the 892 bp in the presence of serum. cAMP activates renin mRNA synthesis parallel to renin production (20-fold increase) as well renin promoter activity (2-fold). cAMP response element and the (-77 to -67) element are both necessary for activation of the renin promoter but do not act independently. Functional analysis of Intron A revealed the presence of a silencer specific to renin-producing cells.
Germain
S
S
INSERM Unit 36, Collège de France, Paris.
Philippe
J
J
Fuchs
S
S
Lengronne
A
A
Corvol
P
P
Pinet
F
F
D000818:Animals; D016466:CHO Cells; D005576:Colforsin / Q000494:pharmacology; D006224:Cricetinae; D000242:Cyclic AMP / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008175:Lung Neoplasms; D011401:Promoter Regions, Genetic; D012083:Renin / Q000235:genetics* / Q000378:metabolism*; D017384:Sequence Deletion; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D005576:Colforsin; D000242:Cyclic AMP; D008156:Luciferases; D012083:Renin