title: Squamoid features and expression of involucrin in primary breast carcinoma associated with high histological grade, tumour cell necrosis and recurrence sites.
abstract: Although breast carcinomas are considered to originate from glandular epithelial cells, some exhibit 'squamoid features', comprising stratification with a gradient in the nuclear-cytoplasmic ratio within individual cancer cell nests on microscopy. In parallel with a histological review of squamoid features, we immunohistochemically investigated the expression of involucrin, a marker of terminal squamous differentiation, in 223 breast carcinomas with one to three regional nodal metastases but no distant metastases and analysed their association with other clinicopathological parameters to explore their clinical and biological implications. Squamoid features and involucrin expression, detected in 22% and 27% of cases respectively, correlated with each other and were associated with high-grade atypia, a solid-nest pattern, cancer cell necrosis on histology and negative oestrogen receptor status. The incidence of regional recurrences was higher in patients with involucrin expression, whereas bone metastases were less frequent in groups with squamoid features or with diffuse (> or = 10%) involucrin expression. Both squamoid features and involucrin expression, which were considered to be derived either from differentiation into keratinocytes or from some kind of cellular degeneration caused by high turnover rate, are suggested to influence the biological behaviour of breast cancer cells in vivo, and they may be effective in predicting the most likely recurrence sites.
authors.affiliation: Pathology Division, National Cancer Center Research Institute and Hospital, Chuo-ku, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tsuda
authors.forename: C
authors.initials: C
authors.lastname: Sakamaki
authors.forename: T
authors.initials: T
authors.lastname: Fukutomi
authors.forename: S
authors.initials: S
authors.lastname: Hirohashi
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009336:Necrosis; D009364:Neoplasm Recurrence, Local / Q000737:chemistry* / Q000473:pathology*; D011498:Protein Precursors / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D011498:Protein Precursors; C032253:involucrin