Changes in osteopontin mRNA expression during phenotypic transition of rabbit arterial smooth muscle cells.
Transition from a contractile to a synthetic phenotype appears to be an early key event during the development of intimal thickening after arterial wall injury. We examined the expression of osteopontin mRNA, proliferation, and phenotypic properties of smooth muscle cells (SMCs) in rabbit neointima after balloon denudation and in primary culture. A strong osteopontin mRNA signal was detected in the thickened intima 1 week after balloon denudation and in the surface layer of the intima 2 weeks after balloon denudation. Ki-67 immunohistochemistry showed that osteopontin mRNA expression increased when SMCs entered the proliferating phase in the intima. Rabbit arterial SMCs on type I collagen after 1 day of primary culture with growth factors, as well as freshly isolated cells, were in the G0 phase (contractile phenotype) and did not express osteopontin mRNA. After 3 days of culture, most cells entered the G1B phase (synthetic phenotype) and expressed osteopontin mRNA. In the absence of growth factors, most cells transferred to the G1A phase (intermediate phenotype) after 3 and 7 days, but did not express osteopontin mRNA. Our findings indicate that the osteopontin gene provides a marker that can be used to distinguish the phenotypic properties of vascular SMCs.
Yamamoto
M
M
Department of Cell Biology, Tokyo Metropolitan Institute of Gerontology, Japan.
Aoyagi
M
M
Azuma
H
H
Yamamoto
K
K
D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D002478:Cells, Cultured; D005434:Flow Cytometry; D016193:G1 Phase; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D053495:Osteopontin; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D016192:Resting Phase, Cell Cycle; D012795:Sialoglycoproteins / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D012795:Sialoglycoproteins; D053495:Osteopontin