title: Isolation and characterization of AFP-binding proteins from tumor and fetal human tissues.
abstract: The AFP receptor (rAFP) was discovered in embryonal and tumor tissues with a high level of proliferation. The AFP-binding protein (AFPbp) possibly containing the AFP-receptor (rAFP) was isolated from human embryos and human breast cancer tissue using affinity chromatography on an AFP-Sepharose column. The similarity of molecular weight, subunit composition, and immunological characteristics was shown for embryonal and tumor AFPbp using immunoblotting, gel-filtration, and PAAG electrophoresis. Judging from Superose-12 gel-filtration data, the protein molecular weight made up to 320-380 kDa. The presence of an IgG-binding site was detected in embryonal and tumor AFPbp by Western blot analysis.
authors.affiliation: Moscow Research Institute of Medical Ecology, Russia.
authors.lastname: Kanevsky VYu
authors.forename: L P
authors.initials: LP
authors.lastname: Pozdnyakova
authors.forename: O A
authors.initials: OA
authors.lastname: Aksenova
authors.forename: S E
authors.initials: SE
authors.lastname: Severin
authors.lastname: Katukov VYu
authors.forename: E S
authors.initials: ES
authors.lastname: Severin
mesh_terms: D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D005260:Female; D005333:Fetus; D006801:Humans; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D018000:Receptors, Peptide / Q000737:chemistry / Q000302:isolation & purification; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D009363:Neoplasm Proteins; D018000:Receptors, Peptide; D000509:alpha-Fetoproteins; C042144:alpha-fetoprotein receptor, human