Specific antibody detection in human aspergillosis: a GEMO* multicentre evaluation of a rapid immunoelectrophoresis method (Paragon). Group d'Etude des Mycoses Opportunistes.
A new immunoelectrophoresis system, the Paragon system, was evaluated in three different hospital centres with the aim of improving standardization of the serodiagnosis of human aspergillosis. To select the most efficient antigen, various commercial and home-made antigens were first tested on 19 sera from 19 patients with highly probable aspergillosis. The value measured using the Paragon anti-Aspergillus antibody detection system was then compared with the results obtained by conventional serological diagnostic methods (conventional immunoelectrophoresis, enzyme linked-immunosorbent assay, indirect immunofluorescence): this step was performed using the first 19 sera as well as 16 other sera from 13 patients with suspected aspergillosis. Concordant results were obtained in 28 cases. The discrepancies observed with seven sera were probably related to differences in the nature of the antigens. Paragon immunoelectrophoresis proved to be a practicable technique requiring only a small amount of serum and giving results within a shorter time than competitive methods (24-48 h). Its major drawbacks compared with conventional immunoelectrophoresis are some difficulties in reading, fewer precipitin lines and the relatively high cost of routine analysis.
Persat
F
F
Laboratoire de Parasitologie et Mycologie Médicale, Université Claude Bernard Lyon I, France.
Gari-Toussaint
M
M
Lebeau
B
B
Cambon
M
M
Raberin
H
H
Addo
A
A
Picot
S
S
Piens
M A
MA
Blancard
A
A
Mallié
M
M
Bastide
J M
JM
Grillot
R
R
D000908:Antibodies, Fungal / Q000097:blood*; D000918:Antibody Specificity; D000946:Antigens, Fungal / Q000276:immunology; D001228:Aspergillosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D001230:Aspergillus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D011233:Precipitin Tests; D015203:Reproducibility of Results; D012698:Serologic Tests / Q000592:standards
D000908:Antibodies, Fungal; D000946:Antigens, Fungal