Identification of a protein linked to the genomic and subgenomic mRNAs of feline calicivirus and its role in translation.
125I protein labelling of oligo(dT)-selected RNA from feline calicivirus (FCV)-infected cells revealed that the genomic and 2.4 kb subgenomic RNAs of FCV are linked to a 15 kDa protein (VPg). Proteinase K treatment of FCV RNA, to remove VPg, led to a decrease in the translatability of the RNA, but there was no obvious change in the site of RNA initiation. Addition of the cap analogue 7-methylGTP to in vitro translations had no effect on the translation of FCV RNA, suggesting that FCV RNA is translated by a cap-independent mechanism. Further evidence that FCV RNA is translated by an unusual mechanism was obtained by translating FCV RNA in vitro at a range of K+ concentrations. FCV RNA was able to direct translation at K+ concentrations at which cellular RNA translation was inhibited.
Herbert
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TP
Department of Pathology, University of Cambridge, UK.
Brierley
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I
Brown
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TD
D000818:Animals; D017927:Calicivirus, Feline / Q000235:genetics* / Q000502:physiology; D002415:Cats; D002460:Cell Line; D019286:Endopeptidase K / Q000378:metabolism; D011188:Potassium; D014176:Protein Biosynthesis; D012314:RNA Cap Analogs / Q000494:pharmacology; D012333:RNA, Messenger; D012367:RNA, Viral; D014758:Viral Core Proteins / Q000235:genetics*
D012314:RNA Cap Analogs; D012333:RNA, Messenger; D012367:RNA, Viral; D014758:Viral Core Proteins; C017516:7-methylguanosine triphosphate; D019286:Endopeptidase K; D011188:Potassium