Developmentally regulated expression of peanut agglutinin (PNA)-specific glycans on murine thymocytes.
Intrathymic maturation of T lymphocytes is characterized by variable expression of O-linked Gal beta 1,3GalNAc glycans reactive with peanut agglutinin (PNA) lectin. Recent studies on human thymocytes show that conversion from PNA+ to PNA- phenotype is correlated with increased expression of alpha 2,3 O-linked sialyltransferase (ST), which sialylates Gal beta 1,3GalNAc glycans, masking their binding sites for PNA. Interestingly, alpha 2,3 O-linked ST expression is highest within the regions of the thymus containing the most immature and most mature thymocyte subsets, suggesting that PNA-specific glycans are intermittently masked by sialylation during thymic selection processes. Here, we studied expression of PNA receptors on developing thymocytes in the murine system using thymocytes from both normal mice and transgenic mice that are genetically arrested at the early phases of T cell development. Our results confirm and extend recent findings in the human system by showing that murine T cells sequentially progress from PNAlo-->PNAhi-->PNAlo stages during their differentiation within the thymus. In addition, our data demonstrate that a similar set of polypeptides is variably masked by sialylation throughout T cell development.
Wu
W
W
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1260, USA.
Punt
J A
JA
Granger
L
L
Sharrow
S O
SO
Kearse
K P
KP
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002236:Carbohydrate Conformation; D002478:Cells, Cultured; D005434:Flow Cytometry; D005690:Galactose / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D019887:Peanut Agglutinin; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D015704:CD4 Antigens; D016827:CD8 Antigens; D037102:Lectins; D019887:Peanut Agglutinin; D011134:Polysaccharides; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D005690:Galactose