[Restriction polymorphism of the proto-oncogene c-Ha-ras-1 in patients with multiple primary malignant neoplasms and non-small-cell lung cancer].
Restriction fragment length polymorphism in the human c-Ha-ras-1 locus, associated with a minisatellite sequence, was examined in 45 multiple primary cancer (MPC) patients, 56 patients with squamous cell lung cancer (SCLC), 21 patients with lung adenocarcinoma (LAC), and 53 individuals having no oncopathology. Southern analysis of cellular DNA revealed the presence of 4 common alleles (with collective allele frequency close to 94% in the control group) and a set of rare alleles. Allele a3, (2.1 kb in size under MspI/HpaII digestion) was shown to be more frequent in the MPC than in the control group. The same tendency was observed in the patients with highly differentiated cell lung cancer. An increased frequency of the a4 allele (2.5 kb under MspI/HpaII digestion) was observed in the patients with adenocarcinomas as well as in the patients with metastases and low levels of tumor tissue differentiation. The elevated frequencies of a3 in the MPC group and of a4 in the LAC patients did not correlate with increased risk of the cancers mentioned above but was associated with type of tumor progression. Previously, it was reported that the mini-satellite sequence within the c-Ha-ras-1 locus possesses enhancer activity. Our data indirectly confirm the hypothesis that the efficiency of minisatellite modulator activity is associated with fragment size.
Gaspar'ian
A V
AV
Sel'chuk
V Iu
VIu
Iakubovskaia
M G
MG
Zborovskaia
I B
IB
Tatosian
A G
AG
D000230:Adenocarcinoma / Q000235:genetics; D000483:Alleles; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D005787:Gene Frequency; D011905:Genes, ras; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D009378:Neoplasms, Multiple Primary / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D000090063:Proto-Oncogene Mas
Restriktsionnyĭ polimorfizm protoonkogena c-Ha-ras-1 u patsientov s pervichno-mnozhestvennymi zlokachestvennymi novoobrazovaniiami i nemelkokletochnkym rakom legkogo.