title: Local delivery of interleukin 4 by retrovirus-transduced T lymphocytes ameliorates experimental autoimmune encephalomyelitis.
abstract: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory autoimmune disease of the central nervous system which serves as a model for the human disease multiple sclerosis. We demonstrate here that encephalitogenic T cells, transduced with a retroviral gene, construct to express interleukin 4, and can delay the onset and reduce the severity of EAE when adoptively transferred to myelin basic protein-immunized mice. Thus, T lymphocytes transduced with retroviral vectors can deliver "regulatory cytokines" in a site-specific manner and may represent a viable therapeutic strategy for the treatment of autoimmune disease.
authors.affiliation: Department of Medicine, Stanford University School of Medicine, California 94305-5111, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Shaw
authors.forename: J B
authors.initials: JB
authors.lastname: Lorens
authors.forename: A
authors.initials: A
authors.lastname: Dhawan
authors.forename: R
authors.initials: R
authors.lastname: DalCanto
authors.forename: H Y
authors.initials: HY
authors.lastname: Tse
authors.forename: A B
authors.initials: AB
authors.lastname: Tran
authors.forename: C
authors.initials: C
authors.lastname: Bonpane
authors.forename: S L
authors.initials: SL
authors.lastname: Eswaran
authors.forename: S
authors.initials: S
authors.lastname: Brocke
authors.forename: N
authors.initials: N
authors.lastname: Sarvetnick
authors.forename: L
authors.initials: L
authors.lastname: Steinman
authors.forename: G P
authors.initials: GP
authors.lastname: Nolan
authors.forename: C G
authors.initials: CG
authors.lastname: Fathman
mesh_terms: D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000628:therapy*; D015316:Genetic Therapy; D005822:Genetic Vectors; D007116:Immunization, Passive; D007167:Immunotherapy; D016753:Interleukin-10 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000008:administration & dosage* / Q000096:biosynthesis; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D013601:T-Lymphocytes; D014161:Transduction, Genetic
chemical_list: D004676:Myelin Basic Protein; D011948:Receptors, Antigen, T-Cell; D016753:Interleukin-10; D015847:Interleukin-4