[Chemiluminescent enzyme immunoassay of cholecystokinin in rat plasma using an immuno-affinity column as a pretreatment].
The cholecystokinin (CCK) plasma concentration of a basal level in a normal rat was at a hardly detectable level (a few pg/ml) even using the highly sensitive chemiluminescent enzyme immunoassay. In this paper, we prepared an anti-CCK F(ab')2 fragment which was made by pepsin digestion from the anti-CCK antiserum. The anti-CCK F (ab')2 fragment was immobilized to the Sepharose 4B gel activated BrCN and packed to column. We have extracted and concentrated CCK in the rat plasma using this column and the CCK assayed by the chemiluminescent enzyme immunoassay (CL-EIA) of CCK. The immuno-affinity column extraction system was more accurate and precise than a reversed phase gel extraction system. The mean recovery of CCK from the rat pooled plasma using the immuno-affinity column was 62.2% (n = 12) by the proposed CL-EIA. The mean +/- S. E. (n = 3) of the CCK concentration in the fasted rat plasma is 3.54 +/- 0.10 pg/ml. The plasma CCK concentrations of normal rats and those of camostat administrated rats, could be measured by the proposed CL-EIA using the immuno-affinity column as a pretreatment.
Ohta
T
T
Mitsubishi Chemical Corporation Research, Yokohama Research Center, Japan.
Ohori
Y
Y
Ito
K
K
Tsuji
A
A
Maeda
M
M
D000818:Animals; D002766:Cholecystokinin / Q000097:blood*; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D008163:Luminescent Measurements; D008297:Male; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D002766:Cholecystokinin