Enhancement of interleukin-1 alpha mediated autocrine growth of cultured human keratinocytes by sho-saiko-to.
We investigated the effects of Sho-saiko-to, the most commonly used herbal medicine in Japan, on the production of interleukin (IL)-1 alpha by cultured human epidermal keratinocytes. IL-1 alpha production was significantly promoted by treatment with 100 or 500 micrograms/ml Sho-saiko-to for 24 or 48 hr. Expression of IL-1 alpha receptors was the most markedly upregulated after treatment with 500 micrograms/ml Sho-saiko-to for 24 hr and with 100 or 500 micrograms/ml for 48 hr; these cells showed the characteristics of multilayered differentiated keratinocytes. The presence of an anti-IL-1 alpha antibody during the treatment with 500 micrograms/ml of Sho-saiko-to for 24 or 48 hr or with 100 micrograms/ml for 48 hr significantly down-regulated the synthesis by the keratinocytes and induced damages in them. Keratinocytes treated with Sho-saiko-to might produce IL-1 alpha and express IL-1 alpha receptors. IL-1 alpha may regulate the proliferation and differentiation of keratinocytes after Sho-saiko-to treatment. These findings suggest that Sho-saiko-to enhances the autocrine growth mediated by IL-1 alpha.
Matsumoto
Y
Y
Department of Dermatology, Nagoya University, School of Medicine, Japan.
Kato
M
M
Tamada
Y
Y
Mori
H
H
Ohashi
M
M
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000906:Antibodies / Q000633:toxicity; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000276:immunology / Q000502:physiology; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D008858:Microscopy, Phase-Contrast; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D015854:Up-Regulation
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000906:Antibodies; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; C018142:saiko-keishi-to; D004247:DNA