title: Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology.
abstract: Human papillomavirus type 16 (HPV-16) is associated with abnormal Papanicolou smears, indicative of cervical intraepithelial neoplasia. HPV-16 is the most common genital HPV and is found in up to 40% of young women with normal cervical cytology. In order to investigate whether transcriptionally active HPV-16 infection is associated with abnormal cervical smears, a reverse transcription-nested PCR assay with primers from the E5 open reading frame was developed to detect all HPV-16 early-region mRNA (E-mRNA) transcripts. It was used to study HPV-16-infected women with normal and abnormal cervical cytologies to obtain evidence of active infection. Among HPV-16 DNA-positive women, HPV-16 E-mRNA was detected in 15 of 37 (40.5%) women with abnormal cervical cytology but in only 4 of 35 (11.4%) women with normal cytology (P = 0.007). Thus, HPV-16 E-mRNA transcription is associated with abnormal cervical smears and may have value as a prognostic marker of progressive disease.
authors.affiliation: Department of Gynaecology, UMDS St. Thomas' Campus, London, United Kingdom. c.biswas@umds.ac.uk
authors.forename: C
authors.initials: C
authors.lastname: Biswas
authors.forename: B
authors.initials: B
authors.lastname: Kell
authors.forename: C
authors.initials: C
authors.lastname: Mant
authors.forename: R J
authors.initials: RJ
authors.lastname: Jewers
authors.forename: J
authors.initials: J
authors.lastname: Cason
authors.forename: P
authors.initials: P
authors.lastname: Muir
authors.forename: K S
authors.initials: KS
authors.lastname: Raju
authors.forename: J M
authors.initials: JM
authors.lastname: Best
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic; D014412:Tumor Virus Infections / Q000473:pathology / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000821:virology; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000473:pathology* / Q000821:virology; D000086082:Viroporin Proteins
chemical_list: D015415:Biomarkers; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; C054859:oncogene protein E5, Human papillomavirus type 16; D000086082:Viroporin Proteins