Influence of solvents and detergents on matrix-assisted laser desorption/ionization mass spectrometry measurements of proteins and oligonucleotides.
The effect of solvents was found to be critical for sample preparation in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). For proteins and oligonucleotides the use of 2-propanol/water as a solvent for different matrices can significantly improve the quality of spectra. This effect is demonstrated with proteins ranging in molecular weight from 12 to 150 kDa and with a special 19-mer oligonucleotide. A comparison of MALDI-MS using of 2-propanol as matrix solvent and high-performance capillary electrophoresis resulted in identical relative peak intensities for a p(dT)12-18 oligonucleotide mixture. Additionally, the effect of detergents for characterization of high molecular weight proteins in very dilute solutions was studied with this solvent. It was found that Triton X-100, up to a concentration of 1%, was highly compatible with MALDI measurements and even could improve the quality of spectra. Use of detergents for cell profiling has extended the detectable mass range to about m/z 75,000.
Börnsen
K O
KO
Novartis Pharma Ltd., Basel, Switzerland.
Gass
M A
MA
Bruin
G J
GJ
von Adrichem
J H
JH
Biro
M C
MC
Kresbach
G M
GM
Ehrat
M
M
D000433:1-Propanol / Q000737:chemistry; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003902:Detergents; D019075:Electrophoresis, Capillary; D009841:Oligonucleotides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D005719:gamma-Globulins / Q000737:chemistry
D003902:Detergents; D009841:Oligonucleotides; D011506:Proteins; D012997:Solvents; D005719:gamma-Globulins; D000433:1-Propanol