title: The mouse uracil-DNA glycosylase gene: isolation of cDNA and genomic clones and mapping ung to mouse chromosome 5.
abstract: Uracil-DNA glycosylase (UDG) is the enzyme responsible for the first step in the base-excision repair pathway that specifically removes uracil from DNA. Here we report the isolation of the cDNA and genomic clones for the mouse uracil-DNA glycosylase gene (ung) homologous to the major placental uracil-DNA glycosylase gene (UNG) of humans. The complete characterization of the genomic organization of the mouse uracil-DNA glycosylase gene shows that the entire mRNA coding region for the 1.83-kb cDNA of the mouse ung gene is contained in an 8.2-kb SstI genomic fragment which includes six exons and five introns. The cDNA encodes a predicted uracil-DNA glycosylase (UDG) protein of 295 amino acids (33 kDa) that is highly similar to a group of UDGs that have been isolated from a wide variety of organisms. The mouse ung gene has been mapped to mouse chromosome 5 using fluorescence in situ hybridization (FISH).
authors.affiliation: Department of Medical Biochemistry, University of Calgary, Health Sciences Centre, Alberta, Canada.
authors.forename: P C
authors.initials: PC
authors.lastname: Svendsen
authors.forename: H A
authors.initials: HA
authors.lastname: Yee
authors.forename: R J
authors.initials: RJ
authors.lastname: Winkfein
authors.forename: J H
authors.initials: JH
authors.lastname: van de Sande
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D045647:DNA Glycosylases; D018076:DNA, Complementary / Q000302:isolation & purification*; D005091:Exons; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D011485:Protein Binding / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D051981:Uracil-DNA Glycosidase
chemical_list: D018076:DNA, Complementary; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase