Recognition and conversion of single- and double-stranded oligonucleotide substrates by 8-oxoguanine-DNA glycosylase from Escherichia coli.
The substrate specificity of 8-oxoguanine-DNA glycosylase was studied. The data showed for the first time that the enzyme can cleave single-stranded deoxyoligonucleotides containing an 8-oxoG link. The values of K(m) and Vmax for a range of single-stranded and double-stranded oligonucleotides (23 bp) containing 8-oxoG at different positions of one chain. These parameters consistently depended on the position of 8-oxoG in single-stranded or double-stranded substrates. A possible mechanism responsible for substrate recognition by 8-oxoguanine-DNA glycosylase is described.
Ishchenko
A A
AA
Novosibirsk Institute of Bioorganic Chemistry, Russian Academy of Sciences, Russia.
Bulychev
N V
NV
Maksakova
G A
GA
Johnson
F
F
Nevinsky
G A
GA
D001483:Base Sequence; D043382:DNA-Formamidopyrimidine Glycosylase; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D007700:Kinetics; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013379:Substrate Specificity
D029968:Escherichia coli Proteins; D009838:Oligodeoxyribonucleotides; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase; C468610:DNA-formamidopyrimidine glycosylase, E coli