title: Simian foamy virus isolated from an accidentally infected human individual.
abstract: Evidence for natural foamy virus (FV) infections in humans is still lacking. However, accidental infections of humans with simian FV have been demonstrated by serology and PCR, but all previous attempts to recover infectious virus in such cases have failed. Here we describe the isolation of a simian FV from peripheral blood mononuclear cells (PBMC) of a healthy animal caretaker, who acquired the virus 20 years ago from an African green monkey (AGM) bite. Properties of the human isolate such as host range in cell cultures including human PBMC and ability to induce neutralizing antibodies in the primate host proved to be similar to those of FV obtained from AGM. The genomic sequence of the isolate was found to be virtually identical to the proviral sequence present in the host lymphocytes and related to AGM isolates but distinct from those of all FV isolates handled in the laboratory. For successful virus isolation, it was essential to stimulate the host lymphocytes by phytohemagglutinin and interleukin-2 for 2 weeks prior to cocultivation with permissive cells. In contrast to the situation found in FV-infected monkeys, virus isolation from the saliva of the animal caretaker was not possible, and no evidence for FV transmission to family contacts was obtained. We conclude that, in contrast to active infection in monkeys, FV persists in a state of latency following accidental infection of humans.
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, University of Freiburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schweizer
authors.forename: V
authors.initials: V
authors.lastname: Falcone
authors.forename: J
authors.initials: J
authors.lastname: Gänge
authors.forename: R
authors.initials: R
authors.lastname: Turek
authors.forename: D
authors.initials: D
authors.lastname: Neumann-Haefelin
mesh_terms: D000059:Accidents; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001733:Bites and Stings / Q000382:microbiology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops / Q000382:microbiology*; D006801:Humans; D016273:Occupational Exposure; D010802:Phylogeny; D012192:Retroviridae Infections / Q000209:etiology / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D016092:Spumavirus / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral