The promoter activity of the phospholipase C-gamma2 gene is regulated by a cell-type-specific control element.
We have cloned and characterized a genomic DNA spanning the 5'-flanking region, the first and second exons, and the first intron of the human PLC-gamma2 gene. The proximal upstream region is highly GC-rich and lacks a TATA box, whereas the distal region contains several AT-rich tracts. Multiple transcription initiation sites were identified by primer extension analysis. Based on the transient transfection assays, the major transcriptional activation element was identified between -183 and +43 (G2SE) and a transcriptional repressive element was found between -303 and -184 (G2RE). The expression of PLC-gamma2 in various cell lines was examined using monoclonal anti-PLC-gamma2 antibody. PLC-gamma2 was highly expressed in B-cell lines such as Daudi, SP2, and Ramos cells, whereas it existed at very low levels in Jurkat, 3T3-L1, NBL-7, and C6Bu-1 cells. Moderate levels of PLC-gamma2 were also detected in C2C12, P19, U937, HL60, A431, and PC12 cells. The 4-kb genomic fragment upstream of -1,654 was able to activate transcription from the PLC-gamma2 promoter in Daudi and C2C12 cells, but not in Jurkat cells, which is consistent with the PLC-gamma2 protein expression levels in those cell lines. These results suggest that the cell-type-specific expression of PLC-gamma2 might be attributed to the transcriptional regulation by the upstream cis-element.
Lee
S J
SJ
Department of Life Science, Pohang University of Science and Technology, Korea.
Bahk
Y Y
YY
Yun
D H
DH
Lee
H J
HJ
Lee
Y H
YH
Ryu
S H
SH
Suh
P G
PG
D001402:B-Lymphocytes / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D004252:DNA Mutational Analysis; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D015698:Genomic Library; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D051966:Phospholipase C gamma; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014162:Transfection; D010738:Type C Phospholipases / Q000235:genetics*
D007527:Isoenzymes; D010738:Type C Phospholipases; D051966:Phospholipase C gamma