title: Localized perforation of the cell wall by a major autolysin: atl gene products and the onset of penicillin-induced lysis of Staphylococcus aureus.
abstract: We investigated the cell surface localization of the atl gene products of Staphylococcus aureus exposed to a lytic concentration (4 MIC) of penicillin G (PCG) by means of immunoelectron microscopy using anti-62-kDa N-acetylmuramyl-L-alanine amidase or anti-51-kDa endo-beta-N-acetylglucosaminidase immunoglobulin G. Protein A-gold conjugates reacting with antigen-antibody complex localized at sites of defects of the cell wall at the nascent cross wall. Anti-62-kDa N-acetylmuramyl-L-alanine amidase or anti-51-kDa endo-beta-N-acetylglucosaminidase immunoglobulin G inhibited the decreased turbidity caused by PCG-induced lysis and the formation of defects in the wall. The autolysis-defective mutant, S. aureus RUSAL2 (atl::Tn551), exposed to 4 MIC of PCG resisted autolysis and formation of the wall defect. These results suggest that activation or deregulation of the atl gene products at localized sites where formation of new cross wall was disturbed by PCG causes small defects in the cell wall in situ, eventually leading to general autolysis.
authors.affiliation: Department of Microbiology, Hiroshima University School of Dentistry, Japan. sugai@ipc.hiroshima-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Sugai
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Nakashima
authors.forename: H
authors.initials: H
authors.lastname: Komatsuzawa
authors.forename: A
authors.initials: A
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Oshida
authors.forename: H
authors.initials: H
authors.lastname: Suginaka
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D002473:Cell Wall / Q000502:physiology / Q000648:ultrastructure; D007700:Kinetics; D016253:Microscopy, Immunoelectron; D008954:Models, Biological; D009154:Mutation; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010400:Penicillin G / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*
chemical_list: D000906:Antibodies; D009238:N-Acetylmuramoyl-L-alanine Amidase; D010400:Penicillin G