Triplex DNA: fundamentals, advances, and potential applications for gene therapy.
The ability to target specific sequences of DNA through oligonucleotide-based triple-helix formation provides a powerful tool for genetic manipulation. Under experimental conditions, triplex DNA can inhibit DNA transcription and replication, generate site-specific mutations, cleave DNA, and induce homologous recombination. This review describes the binding requirements for triplex formation, surveys recent advancements in the chemistry and biology of triple helices and considers several potential applications of triplex DNA for use in genetic therapy.
Chan
P P
PP
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven CT 06520-8040, USA.
Glazer
P M
PM
D000818:Animals; D004247:DNA / Q000627:therapeutic use*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides
D009838:Oligodeoxyribonucleotides; C051802:triplex DNA; D004247:DNA