Effect of polydextrose on absorption of triglyceride and cholesterol in mesenteric lymph-fistula rats.
In most experimental designs, the inhibitory effect of water-soluble dietary fibers on lipid absorption is evaluated by the decrease in plasma lipid concentration or the increase in fecal lipid output. The aim of this study was to evaluate the acute effect of a water-soluble polysaccharide, polydextrose, on lipid transport to the mesenteric lymph using lymph-fistula rats. The mesenteric lymph duct of rats was cannulated, and an infusion tube was introduced into the duodenum. After recovery, a lipid emulsion containing radioactive triolein and cholesteryl oleate was infused into the duodenum for 8 hr. The tested group was infused with the lipid emulsion containing 5% or 10% polydextrose as dietary fiber. Samples from the lymph-fistula were collected, and the luminal contents and mucosa were collected at the end of infusion. Lymph flow in the mesenteric lymph decreased in the polydextrose group after the infusion. The amounts of both triglyceride and cholesterol remaining in the lumen were greater in the polydextrose group, due to decreased transport of lipid into the lymph. These effects were dose dependent in the 5% and 10% polydextrose groups. The results of this study indicate that polydextrose retarded the transport of triolein and cholesterol into the mesenteric lymph.
Ogata
S
S
Department of Internal Medicine, Saga Medical School, Japan.
Fujimoto
K
K
Iwakiri
R
R
Matsunaga
C
C
Ogawa
Y
Y
Koyama
T
T
Sakai
T
T
D000818:Animals; D001692:Biological Transport; D002404:Catheterization; D002784:Cholesterol / Q000378:metabolism*; D005402:Fistula; D005936:Glucans / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism; D050356:Lipid Metabolism; D008196:Lymph / Q000378:metabolism*; D008297:Male; D008643:Mesentery; D051381:Rats; D017207:Rats, Sprague-Dawley; D014304:Triolein / Q000378:metabolism*
D005936:Glucans; D014304:Triolein; D002784:Cholesterol; C033375:polydextrose