Digital processing of the current noise evoked by kainate in cerebellar granule cells.
Current induced in cultured cerebellar granule cells by the bath application of kainate (500 microM) was measured using the conventional patch-clamp technique. Two different kinds of responses were observed after the agonist perfusion. Some cells exhibited small inward whole-cell currents: 116 +/- 40 pA (7 cells) at a clamp potential of -60 mV; in other cells, the agonist induced significantly larger currents: 420 +/- 35 pA (6 cells) at a clamp potential of -60 mV. The current flowing in the agonist-activated ionic channels was indirectly estimated by processing the fluctuations of whole-cell current by means of an original parametric method. Mean conductance of the underlying channels was then determined from the single-channel current estimated at different clamp potentials. In the cells exhibiting small inward currents, the mean conductance was equal to 0.5 +/- 0.2 pS (7 cells), whereas in the cells with large inward currents it was 3 +/- 0.4 pS (6 cells). This result gives a coherent explanation of the different kinds of responses observed at macroscopic level in the whole-cell current and confirms that kainate-activated channels can exhibit different levels of conductance.
Cavalcanti
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S
Department of Electronics, Computer Science and Systems, University of Bologna, Italy.
Zona
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C
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D015640:Ion Channel Gating / Q000187:drug effects*; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology; D007608:Kainic Acid / Q000378:metabolism / Q000494:pharmacology*; D008954:Models, Biological; D018408:Patch-Clamp Techniques; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012815:Signal Processing, Computer-Assisted
D007608:Kainic Acid