Helium-neon laser irradiation is not a stressful treatment: a study on heat-shock protein (HSP70) level.
OBJECTIVE
Helium-neon (He-Ne) laser irradiation has been clinically used to reduce chemotherapy-induced mucositis. This work was designed to find out if this treatment is stressful at the cellular level by studying its effects on the level of the stress-inducible heat shock proteins.


METHODS
Human desmodontal and mouse L929 fibroblasts were irradiated using a 60 mW laser by a single application of 1.5 and 3J/cm2 in continuous mode. Heat shock protein level was studied by gel electrophoresis and Western blotting using monoclonal antibodies.


RESULTS
He-Ne treatment does not induce heat shock protein synthesis in human desmodontal nor in mouse fibroblasts at the energy densities used in this study.


CONCLUSIONS
These results indicate that the treatment is not stressful at the cellular level.
Hashieh
I A
IA
Faculté d'Odontologie, Marseille, France.
Tardieu
C
C
Franquin
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000737:chemistry / Q000528:radiation effects*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000528:radiation effects*; D006371:Helium; D006801:Humans; D007834:Lasers; D051379:Mice; D009356:Neon; D013997:Time Factors
D000911:Antibodies, Monoclonal; D018840:HSP70 Heat-Shock Proteins; D006371:Helium; D009356:Neon