title: Adjuvants influence the quantitative and qualitative immune response in BALB/c mice immunized with respiratory syncytial virus FG subunit vaccine.
abstract: The ability of monophosphoryl lipid A (MPL), QS-21 and alum to alter the immunologic response to immunization with respiratory syncytial virus a chimeric FG construct (FG) subunit vaccine was examined in BALB/c mice. FG/MPL, FG/alum, and FG/MPL/QS-21 combinations increased non-neutralizing antibody response, while FG/QS-21 did not. FG subunit vaccine with MPL, QS-21, or both had cytokine responses more closely resembling primary infection than FG/alum, with decreased interleukin-4 mRNA levels and increased IgG2a isotype antibody. The lungs of the mice immunized with FG subunit vaccines showed a heightened inflammatory response to respiratory syncytial virus challenge as compared to live virus immunization. Adjuvants can be used to alter the humoral and cellular responses to RSV subunit immunization.
authors.affiliation: Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-2605, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Neuzil
authors.forename: J E
authors.initials: JE
authors.lastname: Johnson
authors.forename: Y W
authors.initials: YW
authors.lastname: Tang
authors.forename: J P
authors.initials: JP
authors.lastname: Prieels
authors.forename: M
authors.initials: M
authors.lastname: Slaoui
authors.forename: N
authors.initials: N
authors.lastname: Gar
authors.forename: B S
authors.initials: BS
authors.lastname: Graham
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000534:Alum Compounds; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007114:Immunization / Q000379:methods*; D007132:Immunoglobulin Isotypes / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control*; D014765:Viral Vaccines; D014779:Virus Replication
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000914:Antibodies, Viral; D016207:Cytokines; D007132:Immunoglobulin Isotypes; D014765:Viral Vaccines; C041524:aluminum sulfate