title: Endogenous adenosine on membrane properties of CA1 neurons in rat hippocampal slices during normoxia and hypoxia.
abstract: The effects of endogenous adenosine release on CA1 neurons in hippocampal slices were studied under normoxic and hypoxic conditions, by using extra-/intracellular and whole-cell recordings. During normoxia, the adenosine antagonist, 8-(p-sulphophenyl) theophylline (8-SPT) or adenosine deaminase (ADA) potentiated both evoked CA1 EPSPs and spontaneous synaptic activity, but not monosynaptic IPSPs; there was a minimal depolarization (by 1 mV), probably caused by the enhanced synaptic activity, but no increase in input conductance. Under voltage-clamp with KCl electrodes (with holding potential (VH) near -70 mV), hypoxia (4-5 min) elicited a rise in input conductance and an outward current that reversed near -90 mV, in keeping with the activation of K conductance. These effects of hypoxia were partly attenuated by 8-SPT (10 microM). The hypoxia-induced outward current and conductance increase were abolished by 1 mM Ba, being replaced by a small inward current and a conductance decrease. These data indicate that adenosine tonically inhibits excitatory, but not inhibitory, synaptic transmission, has no direct effect on input conductance, and contributes to the hyperpolarization and fall in input resistance induced by hypoxia.
authors.affiliation: Anaesthesia Research Department, McGill University, Montréal, Québec, Canada.
authors.forename: P J
authors.initials: PJ
authors.lastname: Zhu
authors.forename: K
authors.initials: K
authors.lastname: Krnjević
mesh_terms: D000241:Adenosine / Q000378:metabolism / Q000502:physiology*; D000243:Adenosine Deaminase / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism; D058915:Purinergic P1 Receptor Antagonists; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D015221:Potassium Channels; D058915:Purinergic P1 Receptor Antagonists; C031433:8-(4-sulfophenyl)theophylline; D013806:Theophylline; D000243:Adenosine Deaminase; D000241:Adenosine