title: Molecular analysis of the Rhodococcus sp. strain H1 her gene and characterization of its product, a heroin esterase, expressed in Escherichia coli.
abstract: The structural gene for heroin esterase was cloned from Rhodococcus sp. strain H1 and expressed in Escherichia coli BL21(DE3). The purified enzyme was found to be a tetramer with an M(r) of 137,000 and an apparent K(m) of 0.88 mM for 6-acetylmorphine. The G-x-S-x-G motif was observed in the deduced amino acid sequence, suggesting that the enzyme is a serin esterase.
authors.affiliation: Institute of Biotechnology, University of Cambridge, United Kingdom.
authors.forename: D A
authors.initials: DA
authors.lastname: Rathbone
authors.forename: P J
authors.initials: PJ
authors.lastname: Holt
authors.forename: C R
authors.initials: CR
authors.lastname: Lowe
authors.forename: N C
authors.initials: NC
authors.lastname: Bruce
mesh_terms: D000115:Acetylesterase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D004950:Esterases / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D012240:Rhodococcus / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D004950:Esterases; C022635:serine esterase; C090778:heroin esterase; D000115:Acetylesterase