Propagation of granulocytic Ehrlichia spp. from human and equine sources in HL-60 cells induced to differentiate into functional granulocytes.
Ehrlichia spp. from human and equine sources in the northeastern Unites States were detected by PCR, isolated, and propagated in the HL-60 promyelocytic leukemia cell line. Growth of Ehrlichia from both equine and human sources was enhanced by addition of retinoic acid, which causes granulocytic differentiation of the HL-60 cells. DNA sequencing of a portion of the 16S rDNA gene supported the hypothesis that the same pathogen was responsible for both equine and human granulocytic ehrlichiosis.
Heimer
R
R
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520-8034, USA. robert_heimer@qm.yale.edu
Van Andel
A
A
Wormser
G P
GP
Wilson
M L
ML
D000818:Animals; D002454:Cell Differentiation; D004269:DNA, Bacterial / Q000032:analysis; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000382:microbiology / Q000473:pathology*; D006098:Granulocytes / Q000382:microbiology / Q000473:pathology*; D018922:HL-60 Cells / Q000382:microbiology* / Q000473:pathology; D006736:Horses; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial