The qmeA (ts) mutation of Escherichia coli is localized in the fabI gene, which encodes enoyl-ACP reductase.
The phenotypes of temperature-sensitive qmeA and fabI mutants of Escherichia coli appear to be very similar. Furthermore, the qmeA mutation could be complemented by the fabI gene on a plasmid, and the fabI allele derived from the qmeA mutant strain harbours a nucleotide substitution identical to that from a previously characterized fabI mutant. These results show that the qmeA gene is, in fact, identical to the fabI gene, which encodes enoyl-ACP reductase, involved in fatty acid elongation.
Kleerebezem
M
M
Department of Molecular Cell Biology, Utrecht University, The Netherlands.
Heutink
M
M
de Cock
H
H
Tommassen
J
J
D050745:Enoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific); D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D066298:In Vitro Techniques; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype
D010088:Oxidoreductases; D050745:Enoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific)