Real time observation of binding of herpes simplex virus type 1 (HSV-1) to Vero cells and neutralization of HSV-1 by sulfonated human immunoglobulin.
A real time biomolecular interaction assay system involving an optical sensor was applied to the quantitative analysis of the binding of herpes simplex virus type 1 (HSV-1) to Vero cells, and the neutralization antibody titer against this virus with a commercially available sulfonated human immunoglobulin preparation. The virus titers in a viral solution and the neutralizing antibody titer in the human immunoglobulin preparation could be successfully estimated in a short time with this system without any difficult cytopathic effect analysis of cultured cells.
Inoue
K
K
Department of Microbiology, Meiji College of Pharmacy, Setagaya-ku, Tokyo.
Arai
T
T
Aoyagi
M
M
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D015374:Biosensing Techniques; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D000882:Haplorhini; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D007074:Immunoglobulin G / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D013997:Time Factors
D000906:Antibodies; C021306:IgG, sulfonated; D007106:Immune Sera; D007074:Immunoglobulin G