Cell type and spatial location dependence of cytoplasmic viscosity measured by time-resolved fluorescence microscopy.
Information on the cell type and spatial location dependence of cytoplasmic viscosity would be very useful in understanding some of the processes occurring in the cell. For this purpose, fluorescent dye kiton red (sulforhodamine B) was loaded into a variety of cells such as Swiss 3T3 fibroblasts, human mononuclear cells, Sarcoma-180 tumor cells, Chinese hamster ovary cells, plant cells from Digitalis lanata, stamen hair cells of Tradescantia, and guard mother cells of Allium cepa. Space-resolved measurements of cytoplasmic viscosity were carried out by using an experimental set-up wherein a picosecond laser system was coupled with an epifluorescence microscope. The spatial resolution of this set-up was approximately 1.0 micron, and reliable dynamic fluorescence measurements could be obtained from 10(2) to 10(3) fluorescent molecules. Fluorescence lifetime measurements showed that a large fraction (approximately 70%) of kiton red was in the free form. Fluorescence anisotropy decay of kiton red in cells was analyzed by a two population (free and bound) model. The microviscosity of cytoplasm was estimated from the anisotropy decay kinetics of the free probe. It was found that the cytoplasmic viscosity is dependent on both the cell type and spatial location within a cell. Furthermore, both the average value of viscosity and spatial variation within a cell were larger in the plant cells when compared to the animal cells. Model studies in various simpler systems have shown that the higher viscosity observed in some part of the cell could be due to either physical restriction and/or the presence of high concentrations of small solutes and macromolecules.
Srivastava
A
A
Chemical Physics Group, Tata Institute of Fundamental Research, Mumbai, India.
Krishnamoorthy
G
G
D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003593:Cytoplasm / Q000502:physiology*; D004058:Diffusion; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D010944:Plants; D013997:Time Factors; D014783:Viscosity
D005456:Fluorescent Dyes