[Combination of the sieve effect and ion exchange chromatography during isolation of recombinant alpha2-interferon on the cellosorbent CS KU-23].
Preliminary purification of alpha 2-interferon from the culture liquid on cation-exchange sorbents was studied. Application of a cellosorbent based on the cationite KU-23 resulted in a 20-25-fold purification of recombinant alpha 2-interferon in a one-step process as a result of combination of the sieve effect and ion-exchange chromatography.
Demin
A A
AA
Samsonov
G V
GV
Pirogov
V S
VS
Papukova
K P
KP
D002482:Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000302:isolation & purification*; D011994:Recombinant Proteins
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D002482:Cellulose
Sochetanie sitovogo éffekta i ionoobmennoĭ khromatografii v protsesse vydeleniia rekombinantnogo alpha2-interferona na tsellosorbente CS KU-23.