Reduction of wall adsorption in capillary zone electrophoresis of a basic single-chain antibody fragment by a cationic polymeric buffer additive.
Reduction of adsorptive protein-wall interactions by poly(diallyldimethyl ammonium chloride), a permanently cationic polymer, at a concentration of 0.5% (w/v) is demonstrated for a basic single-chain antibody fragment (scFv, pI about 9.5) even in the range of physiological pH of around 7. The polymer additive forms a positively charged layer at the silica surface which reverses electroosmosis and leads to electrostatic repulsion of the positively charged basic protein.
Morand
M
M
Institute of Analytical Chemistry, University of Vienna, Austria.
Blaas
D
D
Kenndler
E
E
D000327:Adsorption; D002021:Buffers; D002412:Cations; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D007128:Immunoglobulin Fragments / Q000032:analysis*; D007526:Isoelectric Point; D011095:Polyethylenes / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*
D002021:Buffers; D002412:Cations; D007128:Immunoglobulin Fragments; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride