Leptin receptor of Zucker fatty rat performs reduced signal transduction.
Zucker fatty (fa/fa) rats exhibit overt obesity, hypercholesterolemia, hyperlipidemia, and hyperglycemia as recessive traits. The fa mutation has been determined to be a missense mutation in the extracellular domain of the leptin receptor. We report herein the construction of CHO cells that stably express the fa-type leptin receptor and the characterization of this receptor using mRNA expression levels of the immediate early genes, c-fos, c-jun, and jun-B, which are induced by leptin as a criterion of signal transduction. The fa-type receptor not only exhibits a slightly reduced leptin-binding affinity, but also performs reduced signal transduction.
Yamashita
T
T
Department of Laboratory Medicine, School of Medicine, the University of Tokushima, Kawauchi-cho, Japan.
Murakami
T
T
Iida
M
M
Kuwajima
M
M
Shima
K
K
D000818:Animals; D016466:CHO Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000235:genetics*; D017781:Genes, Immediate-Early / Q000235:genetics*; D016762:Genes, fos / Q000235:genetics; D016758:Genes, jun / Q000235:genetics; D007457:Iodine Radioisotopes; D020738:Leptin; D011506:Proteins / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011924:Rats, Zucker; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D015398:Signal Transduction; D014162:Transfection / Q000235:genetics
D002352:Carrier Proteins; D007457:Iodine Radioisotopes; D020738:Leptin; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D011994:Recombinant Proteins