The effect of neuropeptides kyotorphin and neokyotorphin on proliferation of cultured brown preadipocytes.
Stimulation of DNA and protein synthesis in brown preadipocytes by 1 microM neokyotorphin in serum-containing media was comparable with the effect of 1 microM norepinephrine. In serum-free medium a decrease and a shift of the maximal effect to lower concentration of neokyotorphin were observed. Kyotorphin had no effect on cell proliferation in either medium; however, 0.01-1 microM kyotorphin inhibited the cell proliferation stimulated by 1 microM norepinephrine. Norepinephrine and both peptides stimulated comparable Ca2+ rise in freshly isolated brown preadipocytes. The effects of neokyotorphin and norepinephrine were additive, whereas 0.03-0.3 microM kyotorphin blocked the action of 3 microM norepinephrine. The peptides did not affect the cAMP level in non-stimulated or norepinephrine-stimulated cultured cells. The effects of the peptides on the brown fat cell cultures indicate that peripheral tissue cells contain receptors for these neuropeptides.
Bronnikov
G
G
The Wenner-Gren Institute, Stockholm University, Sweden. gennady@molbio.su.se
Dolgacheva
L
L
Zhang
S J
SJ
Galitovskaya
E
E
Kramarova
L
L
Zinchenko
V
V
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004723:Endorphins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009638:Norepinephrine / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*
D016895:Culture Media, Serum-Free; D004723:Endorphins; C019448:kyotorphin; C036450:neo-kyotorphin; D002118:Calcium; D009638:Norepinephrine