Interleukin 4 suppresses primary interferon gamma response by T cells immunized in vivo and cultured in vitro with interleukin 2.
This paper describes a novel primary in vivo/in vitro culture system which allows analysis of the effect of IL-4 added to culture 1 day after immunization on the production of IFN-gamma. Mice are immunized epicutaneously with picryl chloride (TNP) and draining lymph node cells were harvested 1 day later. These cells (1 day lymph node cells), when cultured in vitro for 3 days in the presence of IL-2, either continuously or as a pulse, give an IFN-gamma response on reexposure to antigen 3 days later. This production of IFN-gamma is both antigen-specific and genetically (MHC)-restricted and is due to both CD8+ and CD4+ T cells. However, if 1 day lymph node cells are cultured with both IL-2 and IL-4, no IFN-gamma is produced on subsequent reexposure to antigen, but the cells acquire the ability to produce IL-4 and IL-10. Moreover, cells pulsed with IL-4 blocked IFN-gamma production when cocultured with cells pulsed with IL-2. IL-4 only exerted its inhibitory activity on IFN-gamma production when added on the first or second day of culture and had no effect at later times. Finally, the inhibitory activity of IL-4 on IFN-gamma production may depend on the production of IL-10, induced by the IL-4, as the inhibitory effect of IL-4 is reversed by mAb against IL-10.
Dieli
F
F
Institute of General Pathology, University of Palermo, Italy.
Asherson
G L
GL
Sireci
G
G
Bonnano
C T
CT
Caccamo
N
N
Salerno
A
A
D000818:Animals; D002478:Cells, Cultured; D003864:Depression, Chemical; D000939:Epitopes; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000378:metabolism / Q000502:physiology; D007376:Interleukin-2 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D007700:Kinetics; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008285:Major Histocompatibility Complex / Q000235:genetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010853:Picryl Chloride; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D000939:Epitopes; D007376:Interleukin-2; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma; D010853:Picryl Chloride