title: The branched-chain amino acid permease gene of Saccharomyces cerevisiae, BAP2, encodes the high-affinity leucine permease (S1).
abstract: The amino acid leucine has been shown previously to be transported into a yeast cell by at least three permeases: the general amino acid permease, a high-affinity permease (S1) and a low-affinity permease (S2). We isolated the gene BAP2 as a multicopy suppressor of the YPD- phenotype of aat1leu2 yeast. BAP2 has been identified previously as encoding an amino acid permease which transports branched-chain amino acids. In order to align the genetic and biochemical studies of leucine uptake we completed a detailed kinetic analysis of yeast strains in which the BAP2 gene was disrupted and compared this to the kinetics of uptake of the parental strain. We demonstrate that BAP2 encodes the high-affinity leucine permease previously called S1.
authors.affiliation: Department of Biology, Hamilton College, Clinton, New York 13323, USA.
authors.forename: J
authors.initials: J
authors.lastname: Schreve
authors.forename: J M
authors.initials: JM
authors.lastname: Garrett
mesh_terms: D026905:Amino Acid Transport Systems; D005800:Genes, Fungal / Q000502:physiology; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D026905:Amino Acid Transport Systems; C097094:BAP2 protein, S cerevisiae; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D007930:Leucine