Condensation of plasmid DNA with polylysine improves liposome-mediated gene transfer into established and primary muscle cells.
Cationic liposomes provide a means to introduce genes into cells both ex vivo and in vivo. In the past few years their use has been described in several tissues, e.g. lungs, liver, endothelium, brain. In this study we evaluated a commercially available poly-cationic liposome formulation in delivering a reporter gene into cultured myogenic cell lines from mouse and rat, and primary fetal human myoblasts. We also examined the effect of serum on liposome-mediated transfection and designed a new procedure to enhance transfection efficiency, based on the pre-condensation of plasmid DNA with polylysine. Polylysine pre-condensation was particularly effective when transfecting the cells in the presence of serum, a finding that could be significant for in vivo transfections.
Vitiello
L
L
Department of Genetics, Research Institute, Hospital for Sick Children, Toronto, ON, Canada.
Chonn
A
A
Wasserman
J D
JD
Duff
C
C
Worton
R G
RG
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics* / Q000648:ultrastructure; D016503:Drug Delivery Systems; D018014:Gene Transfer Techniques; D006801:Humans; D008081:Liposomes; D051379:Mice; D018482:Muscle, Skeletal; D010957:Plasmids / Q000235:genetics*; D011107:Polylysine; D051381:Rats
D008081:Liposomes; D011107:Polylysine; D004247:DNA