A 12 kb nucleotide sequence containing the alanine dehydrogenase gene at 279 degrees on the Bacillus subtilis chromosome.
In the framework of the European project aimed at the sequencing of the Bacillus subtilis genome, a DNA fragment of 12315 bp was cloned and sequenced. The DNA fragment is located between rrnB (275 degrees) and pai (284 degrees). Twelve ORFs were predicted to encode putative proteins. Two of these (ald and yukl) coincided with known B. subtilis genes. The products of two other genes (yukK and yukL) showed significant similarity to known proteins present in databases, e.g. pyoverdin synthase of Pseudomonas aeruginosa and pristinamycin synthase D of Streptomyces pristinaespiralis.
Oudega
Bauke
B
Department of Molecular Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, Faculty of Biology, Vrije Universiteit, De Boelelaan1087, 1081 HV Amsterdam, The Netherlands.
Vandenbol
Micheline
M
Department of Microbiology, Faculty of Agronomy, 6 Avenue du Maréchal Juin, B-5030 Gembloux, Belgium.
Koningstein
Gregory
G
Department of Molecular Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, Faculty of Biology, Vrije Universiteit, De Boelelaan1087, 1081 HV Amsterdam, The Netherlands.
D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D016384:Consensus Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; D010453:Peptide Synthases; C104102:pristinamycin I synthetase; C091136:pyoverdine synthetase protein, Pseudomonas