Identification of Salmonella abortusovis by PCR amplification of a serovar-specific IS200 element.
Field and collection isolates of Salmonella abortusovis carry one IS200 element in a distinct chromosome location. IS200 is not found in the corresponding region of the chromosome of other Salmonella serovars. Sequencing of the boundaries of the S. abortusovis-specific IS200 insertion permitted the design of primers for the amplification of this IS200 element by PCR. Isolates of S. abortusovis are identified by the amplification of a DNA fragment of about 900 bp or larger. PCR amplification of DNA from salmonellae other than S. abortusovis yields either a fragment of about 200 bp or no product. The high specificity of the assay is confirmed by the absence of cross-reactivity with the following templates: (i) sheep DNA, (ii) DNAs from abortion-causing agents other than S. abortusovis, and (iii) DNAs from microorganisms that do not cause abortion but are common in flocks.
Beuzón
C R
CR
Departmento de Genética, Universidad de Sevilla, Spain.
Schiaffino
A
A
Leori
G
G
Cappuccinelli
P
P
Rubino
S
S
Casadesús
J
J
D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003429:Cross Reactions / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000235:genetics*; D012680:Sensitivity and Specificity; D012756:Sheep
D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial