title: Effect of consecutively applied fluorescein eye drops on corneal and aqueous concentrations of fluorescein.
abstract: The main objective of this study was to determine the intraocular concentration of fluorescein after applying five eye drops with only 1-min intervals in human eyes. The concentration of fluorescein in corea and aqueous was studied with fluorophotometry in 11 healthy volunteers. Five 10-microliter drops of 2% fluorescein solution were administered with 1-min intervals, and the intraocular concentrations that were achieved were compared with those obtained with just one eye drop. Five consecutive fluorescein drops caused a 2.6- to 3.1-fold increase in fluorescein concentrations of the anterior chamber 1-8 h after application compared with a single eye drop (p < 0.05). Corresponding values for the cornea were 2.1-2.8. As a conclusion, administration of five eye drops applied consecutively significantly increases intraocular drug concentration during 8 h in humans.
authors.affiliation: Department of Ophthalmology, Umeå University, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Lindén
authors.forename: A
authors.initials: A
authors.lastname: Alm
mesh_terms: D000328:Adult; D001082:Aqueous Humor / Q000378:metabolism*; D003315:Cornea / Q000378:metabolism*; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D015590:Fluorophotometry; D006801:Humans; D008297:Male; D009994:Osmolar Concentration
chemical_list: D005452:Fluoresceins; D019793:Fluorescein