Characterization of yellowfin tuna (Thunnus albacares, Scombroidei) tryptophan hydroxylase.
Tryptophan hydroxylase (EC 1.14, 16.4) was purified from yellowfin tuna liver and properties of this enzyme were compared with those of tryptophan hydroxylase from some other species (mouse mastocytoma and rat brain-stem). The molecular weight of the yellowfin tuna enzyme was estimated to be about 280,000 Da. This value is similar to that for the enzymes from mouse mastocytoma and rat brain-stem. On SDS-polyacrylamide gel electrophoresis analysis, yellowfin tuna enzyme was estimated to be about 96,000 Da. This value is different from that for the enzymes from mouse mastocytoma (53,000 Da) and rat brain-stem (59,000 Da) and suggests that yellowfin tuna enzyme may be a dimer of identical subunits of Mr 96,000 Da.
Nagai
T
T
Department of Food Science and Technology, National Fisheries University, Shimonoseki, Japan. machin@fish.u.ac.jp
Hamada
M
M
Kai
N
N
Tanoue
Y
Y
Nagayama
F
F
D000818:Animals; D001933:Brain Stem / Q000201:enzymology; D019281:Dimerization; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D012515:Mast-Cell Sarcoma / Q000201:enzymology; D008670:Metals / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D051381:Rats; D013045:Species Specificity; D013696:Temperature; D014365:Tryptophan Hydroxylase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D014413:Tuna / Q000378:metabolism*
D008670:Metals; D014365:Tryptophan Hydroxylase