Evaluation of an avidin-biotin-peroxidase method with a monoclonal antibody to type IV collagen in the differential diagnosis of bullous pemphigoid and epidermolysis bullosa acquisita.
There are reports in which an immunohistochemical technique with a monoclonal antibody to type IV collagen has been employed for differentiating between bullous pemphigoid (BP) and epidermolysis bullosa acquisita (EBA). The aim of this study was to determine whether this method could be used routinely. Biopsies (paraffin-embedded lesional skin containing a blister) from currently diagnosed patients with clinical features suggesting BP or EBA were examined by an avidin-biotin-peroxidase (ABC) technique. Sera were tested by indirect immunofluorescence on salt-split skin (IF) and immunoblotting (IB). In all cases which exhibited clear type IV collagen staining, the results of the ABC technique agreed with results of both IF and IB. In one confirmed EBA case, it was impossible to unequivocally localize type IV collagen, because it stained very faintly. Taking into consideration the results of our study, data indicating that the level of blistering might not coincide with the localization of immunoreactants in EBA cases and the possibility of an enzymatic destruction of lamina densa, we conclude that the ABC method is unsuitable for differentiation between BP and EBA.
Bowszyc-Dmochowska
M
M
Department of Dermatology, University School of Medicine, Poznań, Poland.
Hashimoto
T
T
Dmochowski
M
M
Nishikawa
T
T
D000911:Antibodies, Monoclonal; D001485:Basement Membrane / Q000201:enzymology / Q000473:pathology; D001706:Biopsy; D001768:Blister / Q000201:enzymology / Q000473:pathology; D003094:Collagen / Q000032:analysis*; D004396:Coloring Agents; D003937:Diagnosis, Differential; D004817:Epidermis / Q000201:enzymology / Q000473:pathology; D016107:Epidermolysis Bullosa Acquisita / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D010391:Pemphigoid, Bullous / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D012867:Skin / Q000201:enzymology / Q000473:pathology
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D007074:Immunoglobulin G; D003094:Collagen