The outer membrane of lipid A-deficient Escherichia coli mutant LH530 has reduced levels of OmpF and leaks periplasmic enzymes.
We have previously described a new Escherichia coli K-12 mutant, LH530, which has a defective outer membrane. LH530 is very sensitive to hydrophobic antibiotics, does not grow at 42 degrees C and synthesizes reduced amounts of lipid A. Phenotypically LH530 is very similar to the known lipid A biosynthesis mutants of E. coli and Salmonella typhimurium. Its genetic defect is not known, but the defect is suppressed by multiple copies of ORF195. Here we show that at 37 degrees C LH530 contains a reduced amount of the OmpF porin and that it leaks periplasmic beta-lactamase at 37 degrees C and 42 degrees C. We further show that ORF195, when present at low copy number, restores the antibiotic resistance and lipid A biosynthesis of LH530 at 28 degrees C, but not at higher temperatures. In contrast, OmpF expression is restored at 37 degrees C.
Nurminen
Marjatta
M
Department of Bacteriology and Immunology, Haartman Institute, PO Box 21 (Haartmaninkatu 3), SF-00014 University of Helsinki, Helsinki, Finland.
Hirvas
Laura
L
Department of Bacteriology and Immunology, Haartman Institute, PO Box 21 (Haartmaninkatu 3), SF-00014 University of Helsinki, Helsinki, Finland.
Vaara
Martti
M
Department of Bacteriology and Immunology, Haartman Institute, PO Box 21 (Haartmaninkatu 3), SF-00014 University of Helsinki, Helsinki, Finland.
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008050:Lipid A / Q000172:deficiency*; D008563:Membrane Lipids / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis; D010957:Plasmids; D013696:Temperature; D001618:beta-Lactamases / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008050:Lipid A; D008563:Membrane Lipids; D010743:Phospholipids; D001618:beta-Lactamases