Secretion of different types of gelatinases from cultured human keratinocytes.
We investigated gelatinolytic enzymes derived from cultured human keratinocytes. Using a zymograph, we detected 92 kDa and 72 kDa gelatinases as major components and 83 kDa as a minor component from a conditioned cultured medium (BSL-K110, Kyokutoseiyaku Co.) of keratinocytes. After several passages, the 72 kDa band disappeared, and the 83 kDa band became dominant in another conditioned culture medium (K-GM, Kurashikibouseki Co.); these keratinocytes had a relatively differentiated appearance. These results suggest that some mechanism may regulate the secretion of selected types of gelatinase from keratinocytes under different conditions.
Kobayashi
T
T
Division of Dermatology, Tokyo Daini National Hospital, Japan.
Hattori
S
S
Nagai
Y
Y
Sakuraoka
K
K
Nishikawa
T
T
D002454:Cell Differentiation; D002478:Cells, Cultured; D017364:Collagenases / Q000145:classification / Q000378:metabolism; D004396:Coloring Agents; D003470:Culture Media; D017077:Culture Media, Conditioned; D004591:Electrophoresis, Polyacrylamide Gel; D018093:Gelatinases / Q000145:classification / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000201:enzymology*; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000145:classification / Q000378:metabolism; D010662:Phenylmercuric Acetate / Q000031:analogs & derivatives; D012967:Sodium Dodecyl Sulfate; D013439:Sulfhydryl Reagents
D004396:Coloring Agents; D003470:Culture Media; D017077:Culture Media, Conditioned; D013439:Sulfhydryl Reagents; D012967:Sodium Dodecyl Sulfate; C010024:4-aminophenylmercuriacetate; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D010662:Phenylmercuric Acetate