title: A novel HSP70 gene of Schizosaccharomyces pombe that confers K-252a resistance.
abstract: A new gene encoding a heat shock protein 70 family protein of Schizosaccharomyces pombe (Sp), named sks2+, was cloned as a weak suppressor for the K-252a-sensitive mutation, ucm1. The nucleotide sequence of sks2+ revealed an open reading frame of a 613-amino-acid (aa) protein. The deduced aa sequence of sks2+ showed significant homology with Saccharomyces cerevisiae (Sc) Ssb1p and Ssb2p responsible for protein synthesis by non-organelle-localized ribosomes, as well as with other proteins of the HSP70 family. The cells lacking the functional sks2+ gene were viable and showed no increased sensitivity to K-252a but grew slowly with an elongated morphology. These results suggest that the sks2+ gene product plays a role in the cell cycle progression and is able to confer drug resistance in a multicopy state.
authors.affiliation: Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Usui
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Kasahara
authors.forename: A
authors.initials: A
authors.lastname: Honda
authors.forename: T
authors.initials: T
authors.lastname: Beppu
authors.forename: S
authors.initials: S
authors.lastname: Horinouchi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002227:Carbazoles / Q000494:pharmacology*; D002453:Cell Cycle / Q000235:genetics; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D005800:Genes, Fungal / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D026121:Indole Alkaloids; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics* / Q000502:physiology
chemical_list: D002227:Carbazoles; D005656:Fungal Proteins; D018840:HSP70 Heat-Shock Proteins; D026121:Indole Alkaloids; C049985:staurosporine aglycone; D011493:Protein Kinase C