title: The in vitro effect of Khawia sinensis on leucocyte activity in carp (Cyprinus carpio).
abstract: The interactions between Khawia sinensis (Cestoda: Caryophyllidea), a pathogenic tapeworm of carp, and host leucocytes has been investigated in vitro by monitoring blastogenesis in pronephric, splenic and thymic lymphocytes and apoptosis of pronephric leucocytes. Both parasite homogenate and excretory/secretory (E/S) products were found to suppress lymphocyte blastogenesis at high concentrations (346.6-98.8 microg ml-1) whilst cell stimulation occurred at low concentrations (49.4-19.8 microg ml-1). This differential affect on leucocyte activity may not be associated with apoptosis in vitro as the parasite E/S products did not significantly affect programmed cell death as monitored by acridine orange and DNA analysis. The parasite effect on leucocyte activity is discussed and its relationship to host susceptibility is evaluated.
authors.affiliation: Parasitology Research Group, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Morley
authors.forename: D
authors.initials: D
authors.lastname: Hoole
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D017209:Apoptosis; D002347:Carps / Q000276:immunology* / Q000469:parasitology; D002478:Cells, Cultured; D002590:Cestode Infections / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology* / Q000469:parasitology; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D000947:Antigens, Helminth