In vitro formation of crocetin glucosyl esters by Crocus sativus callus extract.
Callus extracts of Crocus sativus exhibited the ability to transform all-trans-crocetin into its related glycosides between pH 7.0 and 7.6 in the presence of uridine-diphospho-glucose. The reactions involved the step-wise addition of a glucose moiety to a free carboxyl function and the 1-->6 addition of a glucose moiety to a glucosyl ester function. The kinetics of synthesis for each glycoside seemed to indicate that two distinct glucosyl transferases were implicated in the synthesis of crocin, all-trans-crocetin di-(beta-D-gentiobiosyl) ester.
Dufresne
C
C
Food Research and Development Centre, Agriculture and Agri-Food Canada, St-Hyacinthe, Québec, Canada.
Cormier
F
F
Dorion
S
S
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology*; D004952:Esters; D006027:Glycosides / Q000737:chemistry; D014801:Vitamin A / Q000031:analogs & derivatives
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D004952:Esters; D006027:Glycosides; C487773:trans-sodium crocetinate; D014801:Vitamin A; D002338:Carotenoids