Bovine kidney low molecular weight acid phosphatase: FMN-dependent kinetics.
A low molecular weight bovine kidney acid phosphatase, electrophoretically homogeneous and with a relative molecular mass of 17.8 kDa, was used in this work. Among the various substrates tested, FMN was found to be the most effective, at pH 7.0. Distinct activation energy values were obtained for p-nitrophenyl phosphate- (45.44 kJ mol-1) and flavin mononucleotide- (28.60 kJ mol-1) hydrolysis reactions. The FMN hydrolysis was strongly inhibited by Cu2 and pCMB, but activated by guanosine. Pyridoxal-phosphate and vanadate were competitive inhibitors for the FMN-dependent reaction.
Granjeiro
J M
JM
Departamento de Bioquímica, Universidade Estadual de Campinas, São Paulo, Brasil.
Ferreira
C V
CV
Jucá
M B
MB
Taga
E M
EM
Aoyama
H
H
D000135:Acid Phosphatase / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002729:Chloromercuribenzoates / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005486:Flavin Mononucleotide / Q000737:chemistry*; D006151:Guanosine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D007668:Kidney / Q000737:chemistry* / Q000201:enzymology*; D007700:Kinetics; D008970:Molecular Weight; D009281:Naphthalenes / Q000737:chemistry; D009596:Nitrophenols / Q000737:chemistry; D010755:Organophosphates / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D019000:Phosphotyrosine / Q000737:chemistry; D011732:Pyridoxal Phosphate / Q000737:chemistry; D013379:Substrate Specificity; D014638:Vanadates / Q000737:chemistry; D020245:p-Chloromercuribenzoic Acid
D002729:Chloromercuribenzoates; D007202:Indicators and Reagents; D009281:Naphthalenes; D009596:Nitrophenols; D010755:Organophosphates; D009943:Organophosphorus Compounds; D006151:Guanosine; C107072:beta-naphthyl phosphate; D019000:Phosphotyrosine; C008644:nitrophenylphosphate; D014638:Vanadates; D020245:p-Chloromercuribenzoic Acid; D011732:Pyridoxal Phosphate; D003300:Copper; D005486:Flavin Mononucleotide; D000135:Acid Phosphatase