Identification of Angiostrongylus cantonensis in infected rats with a coagglutination assay.
The presence of Angiostrongylus cantonensis excretory secretory (ES) products was detected in the cerebrospinal fluid (CSF) of infected rats using a coagglutination assay. There was clear agglutination in 100% of CSF samples tested of the rats infected with third-stage larvae of A. cantonensis and neither of the 20 non-infected rats produced agglutination. The assay detected 250 ng/ml or more of ES products. This assay is simple, easy to perform with minimum training and requires no equipment.
Rojas Rivero
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Department of Parasitology, Institute of Tropical Medicine Pedro Kouri, Havana, Cuba.
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D000372:Agglutination Tests / Q000379:methods*; D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000134:cerebrospinal fluid*; D001681:Biological Assay; D004195:Disease Models, Animal; D011817:Rabbits; D051381:Rats / Q000469:parasitology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017206:Strongylida Infections / Q000134:cerebrospinal fluid* / Q000276:immunology
D000947:Antigens, Helminth