Analysis of ribosomal and alphoid repetitive DNA by fiber-FISH.
We have used fluorescence in situ hybridization to extended DNA fibers (fiber-FISH) to analyse the alpha-satellite and rDNA arrays on a human chromosome 22. The rDNA could be seen as a string of signals spanning about 430 kb. The bulk of the alphoid DNA was found in a single large array of about 2.6 Mb.
Shiels
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Department of Biochemistry and Molecular Genetics, Imperial College School of Medicine at St. Mary's London, UK.
Coutelle
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Huxley
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D002460:Cell Line; D002892:Chromosomes, Human, Pair 22; D004275:DNA, Ribosomal; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D012091:Repetitive Sequences, Nucleic Acid; D012270:Ribosomes
D004275:DNA, Ribosomal