Isolation and characterization of AFP-binding proteins from tumor and fetal human tissues.
The AFP receptor (rAFP) was discovered in embryonal and tumor tissues with a high level of proliferation. The AFP-binding protein (AFPbp) possibly containing the AFP-receptor (rAFP) was isolated from human embryos and human breast cancer tissue using affinity chromatography on an AFP-Sepharose column. The similarity of molecular weight, subunit composition, and immunological characteristics was shown for embryonal and tumor AFPbp using immunoblotting, gel-filtration, and PAAG electrophoresis. Judging from Superose-12 gel-filtration data, the protein molecular weight made up to 320-380 kDa. The presence of an IgG-binding site was detected in embryonal and tumor AFPbp by Western blot analysis.
Kanevsky VYu
Moscow Research Institute of Medical Ecology, Russia.
Pozdnyakova
L P
LP
Aksenova
O A
OA
Severin
S E
SE
Katukov VYu
Severin
E S
ES
D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D005260:Female; D005333:Fetus; D006801:Humans; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D018000:Receptors, Peptide / Q000737:chemistry / Q000302:isolation & purification; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D009363:Neoplasm Proteins; D018000:Receptors, Peptide; D000509:alpha-Fetoproteins; C042144:alpha-fetoprotein receptor, human