The amino acid sequence of mitogenic lectin-B from the roots of pokeweed (Phytolacca americana).
The complete amino acid sequence of pokeweed lectin-B (PL-B) has been analyzed by first sequencing seven lysylendopeptidase peptides derived from the reduced and S-pyridylethylated PL-B and then connecting them by analyzing the arginylendopeptidase peptides from the reduced and S-carboxymethylated PL-B. PL-B consists of 295 amino acid residues and two oligosaccharides linked to Asn96 and Asn139, and has a molecular mass of 34,493 Da. PL-B is composed of seven repetitive chitin-binding domains having 48-79% sequence homology with each other. Twelve amino acid residues including eight cysteine residues in these domains are absolutely conserved in all other chitin-binding domains of plant lectins and class I chitinases. Also, it was strongly suggested that the extremely high hemagglutinating and mitogenic activities of PL-B may be ascribed to its seven-domain structure.
Yamaguchi
K
K
Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Yurino
N
N
Kino
M
M
Ishiguro
M
M
Funatsu
G
G
D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D037102:Lectins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D037121:Plant Lectins; D018517:Plant Roots / Q000737:chemistry*; D011043:Pokeweed Mitogens; D017386:Sequence Homology, Amino Acid
D037102:Lectins; D010446:Peptide Fragments; D037121:Plant Lectins; D011043:Pokeweed Mitogens; C105830:lectin B, Phytolacca americana