title: Comparison of tissue distribution of two novel serine/threonine kinase genes containing the LIM motif (LIMK-1 and LIMK-2) in the developing rat.
abstract: We previously isolated two novel serine/threonine kinase genes containing the LIM motif (LIMK-1 and LIMK-2) from a rat cDNA library. To examine the functions of these genes, we performed in situ hybridization in the developing rat nervous system. LIMK-1 and LIMK-2 mRNAs mostly co-localized during development and are expressed preferentially in the central nervous system during mid-to-late gestation but the signals decreased during the post-natal period. However, differential gene expression was observed in some nuclei in the CNS; LIMK-1 mRNA was intensely expressed in the facial motor nucleus, the hypoglossal nucleus, deep nuclei of the cerebellum and the layers 3, 5 and 6 of the adult cerebral cortex while only LIMK-2 mRNA was preferentially expressed in the some parts of the epithelium. In the nasal cavity, LIMK-1 and LIMK-2 mRNAs were expressed complementarily. Our results suggest that LIMK-1 and LIMK-2 may have different functions in these regions during development.
authors.affiliation: Department of Cell Science, Institute of Biomedical Science, Fukushima Medical College, Fukushima City, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: I
authors.initials: I
authors.lastname: Okano
authors.forename: K
authors.initials: K
authors.lastname: Mizuno
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
authors.forename: A
authors.initials: A
authors.lastname: Wanaka
mesh_terms: D000375:Aging; D017398:Alternative Splicing; D000818:Animals; D001921:Brain / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D015723:Gene Library; D014644:Genetic Variation; D007668:Kidney / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D054461:Lim Kinases; D009474:Neurons / Q000201:enzymology; D009928:Organ Specificity; D011494:Protein Kinases / Q000096:biosynthesis*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014158:Transcription, Genetic; D016335:Zinc Fingers
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D011494:Protein Kinases; D054461:Lim Kinases; C515894:Limk1 protein, rat; C490644:Limk2 protein, rat; D017346:Protein Serine-Threonine Kinases