Granulocyte colony-stimulating factor and interleukin-6-producing lung cancer cell line, LCAM.
OBJECTIVE
We describe a case of granulocyte colony-stimulating factor (GCSF) and interleukin-6 (IL-6)-producing lung cancer.


METHODS
A 53-year-old man underwent left upper lobectomy under diagnosis of lung cancer. The tumor obtained by a preoperative biopsy was analyzed.


RESULTS
Preoperative data showed leukocytosis with left-shift of leukocytic morphology and thrombocytosis and an elevated serum GCSF level. Histological examination revealed poorly differentiated adenocarcinoma. A cell line, named LCAM, was established from the tumor and the cytokines in the culture medium were measured by enzyme immunoassay. GCSF and IL-6 were produced in large amounts by LCAM, but granulocyte-macrophage colony-stimulating factor (GMCSF) and interleukin-3 (IL-3) were not. A proportion of LCAM expressed GCSF receptor on the cell surface, but IL-6 receptor could not be detected. LCAM proliferation was inhibited in the culture with antihuman GCSF antibody in a dose-dependent manner.


CONCLUSIONS
We suggest that LCAM proliferation is positively regulated by GCSF.
Inoue
M
M
Division of Organ Transplantation, Osaka University Medical School, Japan.
Minami
M
M
Fujii
Y
Y
Matsuda
H
H
Shirakura
R
R
Kido
T
T
D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D015850:Interleukin-6; D016179:Granulocyte Colony-Stimulating Factor