title: The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.
abstract: The docking of transport vesicles with their target membrane is thought to be mediated by p115. We show here that GM130, a cis-Golgi matrix protein, interacts specifically with p115 and so could provide a membrane docking site. Deletion analysis showed that the N-terminus binds to p115, whereas the C-terminus binds to Golgi membranes. Mitotic phosphorylation of GM130 or a peptide derived from the N-terminus prevented binding to p115. The peptide also inhibited the NSF- but not the p97-dependent reassembly of Golgi cisternae from mitotic fragments, unless it was mitotically phosphorylated. Together, these data provide a molecular explanation for the COPI-mediated fragmentation of the Golgi apparatus at the onset of mitosis.
authors.affiliation: Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Lowe
authors.forename: T P
authors.initials: TP
authors.lastname: Levine
authors.forename: C
authors.initials: C
authors.lastname: Rabouille
authors.forename: G
authors.initials: G
authors.lastname: Warren
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line / Q000502:physiology; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008938:Mitosis / Q000502:physiology*; D008969:Molecular Sequence Data; D017830:Octoxynol; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D051381:Rats; D012492:Salts; D033921:Vesicular Transport Proteins
chemical_list: D001324:Autoantigens; D002352:Carrier Proteins; D000077614:Golgi Matrix Proteins; C097741:Golgin subfamily A member 2; D008565:Membrane Proteins; D010446:Peptide Fragments; D012492:Salts; D033921:Vesicular Transport Proteins; C091580:vesicular transport factor p115; D017830:Octoxynol