[Comparison of biological activity of high molecular weight and low molecular weight forms of immunoreactive prolactin from human serum in cultured lymphoma NB2 cells].
In experiments with NB2 rat lymphoma cells culture sensitive to lactogenic hormones the mitogenic activity of high molecular weight (> OOK) immunoreactive prolactin, found in substantial quantities in serum of certain hyperprolactinemic women, as compared to the activity of serum low molecular weight (23K) form, was studied. It was established that the ratio of immunoreactive to biologically active prolactin content in serums in cases of low molecular weight form predominance is close to 1,0 whereas in case of predominant content of high molecular weight form it is substantially higher (1.5-2.3), apparently because of low biological activity of high molecular weight form. Direct comparison of mitogenic effects of equivalent quantities of serum immunoreactive prolactin forms with high and low molecular weight, separated by gel-filtration, confirmed low biological activity of high molecular weight form. Monoclonal antibodies to prolactin completely suppressed mitogenic activity of low molecular weight form and only partially--high molecular weight one. The data obtained indicate that high and low molecular weight forms of human serum immunoreactive prolactin differ in their biochemical and functional characteristics. Therefore their ratio in the circulating blood can substantially affect the clinical manifestations of hyperprolactinemia.
Bulatov
A A
AA
Osipova
T A
TA
Makarovskaia
E E
EE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006801:Humans; D006966:Hyperprolactinemia / Q000097:blood; D008223:Lymphoma; D008970:Molecular Weight; D011388:Prolactin / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; D011388:Prolactin
Sravnenie biologicheskoĭ aktivnosti vysokomolekuliarnoĭ i nizkomolekuliarnoĭ form immunoreaktivnogo prolaktina syvorotki krovi cheloveka v kul'ture kletok NB2 limfomy.