Phosphorimetric determination of dipyridamole in pharmaceutical preparations.
Room temperature phosphorescence was applied to the determination of dipyridamole in pharmaceutical preparations. The response was linear in the concentration range 100-1600 ng ml-1. The use of phosphorescence enhancers such as thallium(I) nitrate (external heavy atom), sodium dodecyl sulfate (microemulsion stabilizer) and sodium sulfite (deoxygenation agent) was studied and optimized to obtain maximum sensitivity and adequate selectivity. The determination was performed in 0.026 M sodium dodecyl sulfate, 0.0156 M thallium nitrate and 0.02 M sodium sulfite. The pH value was 11.5, adjusted by adding sodium hydroxide. The phosphorescence was totally developed in 15 min, after that the intensity was measured at lambda ex = 303 nm and lambda em = 616 nm. The recovery of the method was tested on commercial formulations containing dipyridamole. The recoveries obtained were 94.67 +/- 0.58% for Persantin and 96.75 +/- 1.37% for Asasantin. The overall least squares regression method was applied to find the most exact straight line that fits the experimental data. The detection limit according to the error propagation theory was 16.4 ng ml-1. The repeatability and relative standard deviation were also determined according to this theory.
Murillo Pulgarín
J A
JA
Department of Analytical Chemistry and Foods Technology, University of Castilla La Mancha, Spain.
Alañón Molina
A
A
Fernández López
P
P
D004176:Dipyridamole / Q000032:analysis* / Q000737:chemistry; D049449:Luminescence; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013057:Spectrum Analysis
D004364:Pharmaceutical Preparations; D004176:Dipyridamole