title: Distribution of serotypes, IS901 and a 40 kDa protein in Mycobacterium avium complex strains isolated from man and animals in Denmark.
abstract: The aim of the present study was to characterize all strains of the Mycobacterium avium complex isolated in Denmark in 1993. A total of 141 M. avium complex strains (86 from man, 38 from animals, and 17 from peat) were analysed by serotyping, ELISA specific for a 40 kDa protein, and IS901-specific PCR. Serotype analysis showed that the most frequent serotypes among human strains were serotype 4 (27%) and serotype 6 (19%), which differs from an earlier survey where serotype 1 was most prevalent. The most frequent serotypes in animals were serotype 2 (53%) and serotype 6 (13%), whereas the most prevalent serotypes among strains isolated from peat were serotype 4 (29%) and serotype 9 (18%). There was a concurrent appearance of IS901 and p40 in all strains. Only M. avium complex strains isolated from animals, and belonging to serotype 1 or serotype 2, contained the IS901/p40 markers. The different distribution of serotypes of M. avium complex strains in animals and man, and the presence of IS901/p40 exclusively in animal strains, suggests that transmission of M. avium from animals to man is not of significance in Denmark.
authors.affiliation: Danish Veterinary Laboratory, Copenhagen, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Klausen
authors.forename: S B
authors.initials: SB
authors.lastname: Giese
authors.forename: K
authors.initials: K
authors.lastname: Fuursted
authors.forename: P
authors.initials: P
authors.lastname: Ahrens
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001717:Birds; D002417:Cattle; D017931:DNA Primers; D004251:DNA Transposable Elements; D003718:Denmark; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D006801:Humans; D009162:Mycobacterium avium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology* / Q000635:transmission; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013552:Swine; D014376:Tuberculosis / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D014379:Tuberculosis, Avian / Q000382:microbiology / Q000635:transmission
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004251:DNA Transposable Elements