Cloning and characterization of a novel gene, WS-3, in human chromosome 8p11-p12.
A novel human gene referred to as the WS-3 gene, in the short arm of human chromosome 8, was cloned by a combination of exon trapping, thermal asymmetric interlaced-PCR (TAIL-PCR) and the Marathon-Ready cDNA amplification method. The gene consists of 7 exons separated by 6 introns, and is at the telomere side of the STS marker, D8S1055. The full-length WS-3 gene contains 1052 nucleotides and codes for a protein of 190 amino acids with a calculated mol. wt. of 20,747. Southern blot experiments showed that the WS-3 gene exists as a single copy in the human genome. A protein encoded by the WS-3 gene has an R-G-D (Arg-Gly-Asp) motif in the N-terminal region, which seems to confer adhesive properties to macromolecular proteins like fibronectin. Although WS-3 is a small gene with unknown biological function, its ubiquitous expression in various tissues and organs suggests that the encoded protein is one of the essential components of all organs and tissues.
Ichikawa
K
K
AGENE Research Institute, Kamakura-shi, Kanagawa, Japan.
Yamabe
Y
Y
Imamura
O
O
Kuromitsu
J
J
Sugawara
K
K
Suzuki
N
N
Shimamoto
A
A
Matsumoto
T
T
Tokutake
Y
Y
Kitao
S
S
Kataoka
H
H
Satoh
M
M
Sugimoto
M
M
Goto
M
M
Sugawara
M
M
Furuichi
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D005091:Exons; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D016133:Polymerase Chain Reaction; D014898:Werner Syndrome / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary