title: Interleukin-13 but not interleukin-4 prolongs eosinophil survival and induces eosinophil chemotaxis.
abstract: The effects of recombinant human (rh) interleukin (IL)-4 or rhIL-13 on survival, and chemotactic activity of human eosinophils were examined. Only rhIL-13 prolonged eosinophil survival in a dose-dependent manner above 3 ng/ml. Eosinophil survival induced by rhIL-13 was inhibited by monoclonal antibodies (mAbs) against IL-3 (p < 0.01) and granulocyte-macrophage colony-stimulating factor (GM-CSF) (p < 0.05), suggesting that rhIL-13 induced IL-3 and GM-CSF production from eosinophils and an autocrine mechanism is responsible for the eosinophil survival. The effects of rhIL-13 on eosinophil chemotactic activity were also examined. rhIL-13 showed chemotactic activity for eosinophils in a dose-dependent manner. Checkerboard analysis revealed that eosinophil migration was dependent on the concentration gradient, confirming that rhIL-13 is a chemotactic factor. rhIL-4 showed no effects. IL-13 may play an important role in the survival and recruitment of eosinophils in allergic diseases.
authors.affiliation: First Department of Internal Medicine, Shinshu University School of Medicine, Nagano.
authors.forename: S
authors.initials: S
authors.lastname: Horie
authors.forename: Y
authors.initials: Y
authors.lastname: Okubo
authors.forename: M
authors.initials: M
authors.lastname: Hossain
authors.forename: E
authors.initials: E
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Nomura
authors.forename: S
authors.initials: S
authors.lastname: Koyama
authors.forename: J
authors.initials: J
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Isobe
authors.forename: M
authors.initials: M
authors.lastname: Sekiguchi
mesh_terms: D015550:Cell Degranulation / Q000187:drug effects; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004306:Dose-Response Relationship, Immunologic; D004804:Eosinophils / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D018793:Interleukin-13 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000096:biosynthesis; D015847:Interleukin-4; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D018793:Interleukin-13; D007377:Interleukin-3; D011994:Recombinant Proteins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor