title: The yeast ORF YDL202w codes for the mitochondrial ribosomal protein YmL11.
abstract: The Saccharomyces cerevisiae open reading frame YDL202w has been characterised in the course of the EUROFAN yeast genome analysis program. Disruption of YDL202w causes a respiratory deficient phenotype accompanied by a loss of mitochondrial DNA. This phenotype is usually found in mutants defective in mitochondrial replication or gene expression. YDL202w has the potential to encode a soluble protein of 249 amino acids. It shows significant similarities to the ribosomal protein L10 from various bacteria and to a previously determined amino-terminal peptide sequence of the yeast mitochondrial ribosomal protein L11. The predicted amino-acid sequence of YDL202w starts with a stretch which has neither any correspondence in the bacterial sequences nor in the protein isolated from mitochondrial ribosomes. Furthermore, this stretch matches the requirements for a signal sequence for mitochondrial protein import. A mitochondrial location of the YDL202w gene product was proven by use of a carboxy terminally HA-tagged version. These findings clearly indicate that YDL202w encodes this mitochondrial ribosomal protein (YmL11).
authors.affiliation: Vienna Biocenter, Institute of Microbiology and Genetics, University of Vienna, A-1030 Wien, Austria.
authors.forename: D M
authors.initials: DM
authors.lastname: Bui
authors.forename: E
authors.initials: E
authors.lastname: Jarosch
authors.forename: R J
authors.initials: RJ
authors.lastname: Schweyen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004261:DNA Replication; D005656:Fungal Proteins / Q000235:genetics*; D016681:Genome, Fungal; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010641:Phenotype; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D012269:Ribosomal Proteins