Heterogeneity of ICAM-1 expression, and cytokine regulation of ICAM-1 expression, in skin and oral keratinocytes.
Differences in expression of intercellular adhesion molecule-1 (ICAM-1) and ICAM-1 mRNA levels were studied in cultured skin and oral keratinocytes before and after stimulation with different pro-inflammatory cytokines. Basal expression of ICAM-1 was undetectable on skin keratinocytes but oral keratinocytes expressed ICAM-1 at high levels. Interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) increased ICAM-1 expression on both cell types, although TNF-alpha had a greater effect on oral than skin keratinocytes (P<0.05) and IFN-gamma was more effective than TNF-alpha on both cell types (P<0.01). In combination, TNF-alpha and IFN-gamma synergistically increased ICAM-1 expression on skin keratinocytes only, although ICAM-1 mRNA was synergistically increased in both cell types. IL-1alpha and IL-1beta induced a small increase in ICAM-1 expression on oral keratinocytes but had no effect on skin keratinocytes.
Li
J
J
The Clinical Academic Group of Oral Medicine and Dental Diagnostic Science, University of Manchester School of Dental Science, Manchester, England.
Mahiouz
D L
DL
Farthing
P M
PM
Haskard
D O
DO
Thornhill
M H
MH
D015152:Blotting, Northern; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D004357:Drug Synergism; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000235:genetics*; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D009061:Mouth Mucosa / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016207:Cytokines; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma