Site-specific substitution of inosine at the terminal positions of a pre-mRNA intron: implications for the configuration of the terminal base interaction.
Genetic evidence in yeast has revealed that a non-Watson-Crick base-pairing interaction between terminal guanosine residues of the intron is required for the second step of pre-mRNA splicing. To explore the likely configuration of the interaction between the terminal guanosines of the intron, inosine was uniformly incorporated into an adenovirus pre-mRNA substrate (Ade) to replace guanosine residues. Splicing of the inosine-containing Ade pre-mRNA was completely inhibited. Psoralen cross-linking reveals that the association of U1 and U2 snRNPs with the intron was impaired. To eliminate the deleterious effects caused by complete inosine replacement, guanosine residues at the splice site(s) of the Ade pre-mRNA were substituted by inosine. Such pre-mRNA substrates were obtained by ligation of two or three RNA fragments; the 3' piece was primed with inosine or mono-phosphate inosine. Splicing of the Ade pre-mRNA containing an inosine residue at the 5' or the 3' splice site, or at both sites proceeds normally. Thus, the functions of the terminal guanosine residues of the intron in splicing can be replaced by inosine. This result supports the previous notion that an N1-carbonyl symmetric interaction likely occurs between the intron terminal residues during pre-mRNA splicing.
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Division of Infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Nankang, Taipei, Taiwan.
D001482:Base Composition; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D017124:Conserved Sequence; D017931:DNA Primers; D006151:Guanosine; D006367:HeLa Cells; D006801:Humans; D006860:Hydrogen Bonding; D007288:Inosine; D007438:Introns; D008958:Models, Molecular; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000737:chemistry / Q000378:metabolism*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000737:chemistry
D017931:DNA Primers; D012322:RNA Precursors; D012333:RNA, Messenger; D006151:Guanosine; D007288:Inosine