Establishment and characterization of an epithelioid sarcoma cell line with an autocrine response to interleukin-6.
A novel epithelioid sarcoma (ES) cell line, designated as ES-OMC-MN, was established from a 44-year-old woman with recurrence and metastasis of ES. The cells were spindle-shaped or polygonal and were positive for cytokeratin and vimentin on immunohistochemical staining. Electron microscopy revealed desmosome-like structures between the cells. These characteristics were also noted in the original tumor. Southern blot analysis of HindIII digests showed an additional 8.0 kb band and an 8.8 kb band in DNA from the cultured cells and the original tumor as well as the peripheral blood cells of the patient, while only an 8.8 kb band was observed in control human placental DNA. There were no point mutations of N-ras codons 12, 13, and 61, suggesting that the abnormality of N-ras was not due to mutation but to polymorphism. Interleukin-6 (IL-6) was secreted into the culture medium at high levels. Recombinant IL-6 augmented the proliferation of these cells, while cell growth was inhibited by incubation with an anti-IL-6 antibody. The cells also expressed surface IL-6 receptors, indicating that IL-6 acted on this cell line in an autocrine manner. IL-6 and IL-6 receptors were also found in the original tumor. These results demonstrate that ES-OMC-MN cells retained all the morphological and biochemical characteristics of the original tumor and suggest that an autocrine effect of IL-6 may be involved in the development of ES.
Kusakabe
H
H
Department of Dermatology, Osaka Medical College, Japan.
Sakatani
S
S
Yonebayashi
K
K
Kiyokane
K
K
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006133:Growth Substances / Q000378:metabolism; D006728:Hormones / Q000627:therapeutic use*; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000627:therapeutic use*; D007621:Karyotyping; D008854:Microscopy, Electron; D009857:Oncogenes; D012509:Sarcoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D012983:Soft Tissue Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured
D016207:Cytokines; D006133:Growth Substances; D006728:Hormones; D015850:Interleukin-6