Physical mapping shows that the unstable oxytetracycline gene cluster of Streptomyces rimosus lies close to one end of the linear chromosome.
A restriction map of the 8 Mb linear chromosome of Streptomyces rimosus R6-501 was constructed for the enzymes Asel (13 fragments) and Dral (7 fragments). Linking clones for all 12 Asel sites and 5 of the 6 Dral sites were isolated. The chromosome has terminal inverted repeats of 550 kb, which are the longest yet reported for a Streptomyces species. The oxytetracycline gene cluster lies about 600 kb from one end, which might account for its frequent spontaneous amplification and deletion. Several other markers were localized on the chromosome (dnaA and recA, the rrn operons, the attachment site for pSAM2 and prophages RP2 and RP3). Comparison of the conserved markers with the map of Streptomyces coelicolor A3(2) suggested there are differences in genome organization between the two species.
Pandza
Kenan
K
LB Genetik, Universität Kaiserslautern, Postfach 3049, D-67653 Kaiserslautern, Germany.|PLIVA d.d. Research Institute, Prilaz baruna Filipovića 25, 10000 Zagreb, Republic of Croatia.
Pfalzer
Guido
G
LB Genetik, Universität Kaiserslautern, Postfach 3049, D-67653 Kaiserslautern, Germany.
Cullum
John
J
LB Genetik, Universität Kaiserslautern, Postfach 3049, D-67653 Kaiserslautern, Germany.
Hranueli
Daslav
D
PLIVA d.d. Research Institute, Prilaz baruna Filipovića 25, 10000 Zagreb, Republic of Croatia.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003360:Cosmids / Q000235:genetics; D015723:Gene Library; D005798:Genes, Bacterial; D005819:Genetic Markers; D010118:Oxytetracycline / Q000096:biosynthesis*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification / Q000235:genetics*
D005819:Genetic Markers; D010118:Oxytetracycline