Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit.
Extraction of high-quality RNA from blackcurrant fruit has hitherto proved difficult, probably owing to high levels of phenolic and polysaccharide components in the berries. The procedure described here is a modification of one described for grape berries, and yields RNA suitable for in vitro translations, RNA blot analysis, and cDNA library construction.
Woodhead
M
M
Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Dundee, Scotland, UK.
Taylor
M A
MA
Davies
H V
HV
Brennan
R M
RM
McNicol
R J
RJ
D005638:Fruit / Q000235:genetics*; D018749:RNA, Plant / Q000302:isolation & purification*
D018749:RNA, Plant