Characterization of a mutation in the parE gene that confers fluoroquinolone resistance in Streptococcus pneumoniae.
We report a mutation in the parE genes of two in vitro mutants of Streptococcus pneumoniae responsible for low-level resistance to fluoroquinolones. Sequential acquisition of mutations in parE and gyrA leads to higher levels of resistance. This confirms that topoisomerase IV is the primary target of fluoroquinolones in S. pneumoniae.
Perichon
B
B
Unité des Agents Antibactériens, Centre National de la Recherche Scientifique, EPJ 0058, Institut Pasteur, Paris, France. brunoper@pasteur.fr
Tankovic
J
J
Courvalin
P
P
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D027101:DNA Topoisomerase IV; D004268:DNA-Binding Proteins / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D024841:Fluoroquinolones; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV