The hupC gene product is a component of the electron transport system for hydrogen oxidation in Pseudomonas hydrogenovora.
The hydrogenase gene cluster containing nine genes (hupSLCDFGHIJ) was identified by sequencing of an 8.8-kb DNA region from Pseudomonas hydrogenovora. To investigate the function of the hupC gene product, we isolated a hupC-null mutant (HID3) of P. hydrogenovora by introducing an in-frame deletion into the hupC. The mutant, HID3, could not grow autotrophically but retained half the level of hydrogenase activity of the wild-type strain. Results of the oxygen consumption test and Western blot analysis revealed that the hupC gene product is a b-type cytochrome but not involved in the hydrogenase maturation process.
Ohtsuki
T
T
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Kita
Y
Y
Fujioka
T
T
Hashimoto
D
D
Shimosaka
M
M
Okazaki
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002462:Cell Membrane; D003573:Cytochrome b Group / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport; D004795:Enzyme Stability; D005798:Genes, Bacterial / Q000235:genetics; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D003573:Cytochrome b Group; C074900:hupC protein, Bacteria; D006859:Hydrogen; D006864:Hydrogenase