title: Histologic evaluation during healing of hydrogel core-and-skirt keratoprostheses in the rabbit eye.
abstract: OBJECTIVE
  We developed two models that are modifications of our original poly(2-hydroxyethyl methacrylate) (PHEMA) core-and-skirt keratoprosthesis. In these keratoprostheses, the mechanical strength of the skirt has been considerably increased with divinyl glycol (DVG) as a cross-linking agent during polymerization. In one (KPro I), methyl methacrylate (MMA) was added as comonomer to increase cell adhesion, and in the other (KPro II), HEMA was polymerized with DVG without comonomer. The aim of this study was to evaluate the process of healing and biocolonization and to ascertain whether KPro I demonstrates better ingrowth than the mechanically stronger KPro II, after implantation in rabbit eyes.
  
  
  METHODS
  Ten rabbits were used for each model and studied at five predetermined end points up to 26 weeks. The device was implanted as a full-thickness keratoprosthesis covered with a conjunctival flap.
  
  
  RESULTS
  Neither prosthesis demonstrated extrusion or retroprosthetic membrane formation. There was no significant difference between the two types of prosthesis with respect to tissue ingrowth and surrounding tissue melting. Histologically, inflammation was not severe, but calcification was seen in most specimens. Evidence of biodegradation of the prosthesis also was seen.
  
  
  CONCLUSIONS
  In our original keratoprosthesis, fibrovascular invasion had occurred into the prosthetic skirt, but wound dehiscence and low mechanical strength resulted in an unfavorable outcome. In this series, the mechanical properties were improved, and KPro II was stronger than KPro I. Therefore KPro II would be the preferred polymer combination for surgical manipulation. However, biodegradation and calcification require further investigation into the degree and significance of these adverse reactions.
authors.affiliation: Lions Eye Institute, Perth, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Vijayasekaran
authors.forename: C R
authors.initials: CR
authors.lastname: Hicks
authors.forename: T V
authors.initials: TV
authors.lastname: Chirila
authors.forename: J H
authors.initials: JH
authors.lastname: Fitton
authors.forename: A B
authors.initials: AB
authors.lastname: Clayton
authors.forename: X
authors.initials: X
authors.lastname: Lou
authors.forename: S
authors.initials: S
authors.lastname: Platten
authors.forename: G J
authors.initials: GJ
authors.lastname: Crawford
authors.forename: I J
authors.initials: IJ
authors.lastname: Constable
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D002114:Calcinosis / Q000473:pathology; D003228:Conjunctiva; D003315:Cornea / Q000473:pathology* / Q000601:surgery; D005500:Follow-Up Studies; D005549:Foreign-Body Reaction / Q000473:pathology*; D008689:Methacrylates; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D019736:Prostheses and Implants; D011817:Rabbits; D013524:Surgical Flaps / Q000379:methods / Q000473:pathology; D014945:Wound Healing
chemical_list: D008689:Methacrylates; D008768:Methylmethacrylates; D020366:Methylmethacrylate; C005044:hydroxyethyl methacrylate