Qualitative immunoblot analysis of PKC isoforms expressed in airway smooth muscle.
Protein kinase C (PKC) was originally identified as a single serine/ threonine protein kinase with calcium- and phospholipid-dependent activity, but more recently PKC has been found to consist of a family of multiple isoenzymes with different biochemical characteristics, substrates, and cofactor requirements. PKC is particularly important in regulating airway smooth muscle (ASM) tone. Although a previous investigation has demonstrated PKC-beta, -delta, -epsilon, -theta and -zeta in canine trachealis muscle, additional PKC isoforms have not been characterized in ASM. Therefore, immunoblot analysis using nine isotype-specific antibodies was used to further characterize the expression of PKC isoforms in porcine ASM. In addition to the previously described beta-, delta-, epsilon-, and zeta-isoforms in ASM, the calcium-dependent alpha-isoform, and the calcium- and diacylglycerol-independent isoforms iota/lambda and mu were identified. This study demonstrates multiple PKC isoforms in porcine ASM that can participate in intracellular signaling pathways in this tissue.
Togashi
H
H
Department of Anesthesiology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA.
Hirshman
C A
CA
Emala
C W
CW
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D007527:Isoenzymes / Q000378:metabolism*; D009130:Muscle, Smooth / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D013552:Swine; D014132:Trachea / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011493:Protein Kinase C