title: Effects of fatty acids and hormones on fatty acid metabolism and gluconeogenesis in bovine hepatocytes.
abstract: Primary cultures of hepatocytes were used to study the effects of extracellular oleate concentration and hormones on fatty acid metabolism and gluconeogenesis. Rates of oleate uptake and oxidation to acid-soluble products varied linearly as oleate concentrations increased (0.1 to 2 mM), but rates of triglyceride accumulation varied quadratically. Insulin increased the proportion of oleate that was esterified by 22% without affecting the formation of acid-soluble products. Cells incubated with 2 mM [1-(14)C]oleate for 24 h eliminated 9.6% of the labeled intracellular lipid as acid-soluble products in the following 24 h when no oleate was present during depletion and eliminated 7.7% when 2 mM oleate was present. Insulin reduced labeled triglyceride depletion by 49%. Gluconeogenesis from [2-(14)C] propionate was depressed by 24%, and formation of acid-soluble products was increased by 46% in cells infiltrated with lipid because of previous exposure to 2 mM oleate for 45 h. Rates of gluconeogenesis from propionate were reduced 23% when 2 mM oleate was present during the 3-h period that gluconeogenesis was measured, and the effect was not modified by lipid infiltration. Lipid infiltration influenced hepatic function, and insulin regulated hepatic triglyceride concentration.
authors.affiliation: University of Wisconsin, Madison 53706, USA.
authors.forename: C
authors.initials: C
authors.lastname: Cadórniga-Valiño
authors.forename: R R
authors.initials: RR
authors.lastname: Grummer
authors.forename: L E
authors.initials: LE
authors.lastname: Armentano
authors.forename: S S
authors.initials: SS
authors.lastname: Donkin
authors.forename: S J
authors.initials: SJ
authors.lastname: Bertics
mesh_terms: D000818:Animals; D002417:Cattle / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D004967:Estrogens / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005934:Glucagon / Q000494:pharmacology*; D005943:Gluconeogenesis / Q000187:drug effects* / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007651:Keto Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000493:pharmacokinetics; D008099:Liver / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid / Q000378:metabolism / Q000493:pharmacokinetics; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism
chemical_list: D004967:Estrogens; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D007651:Keto Acids; D008055:Lipids; D014280:Triglycerides; D019301:Oleic Acid; D005934:Glucagon; D005947:Glucose