title: Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium Thermotoga maritima.
abstract: Phosphoglycerate kinase from the hyperthermophilic bacterium Thermotoga maritima has been co-crystallized with its substrate 3-phosphoglycerate and the ATP analogue AMP-PNP using the vapour diffusion method. Crystals were obtained from a solution containing polyethylene glycol (MW 3000/8000) as precipitating agent. A complete diffraction data set from orthorhombic crystals was collected up to 2.0 A resolution. The TmPGK crystallizes in the space group P2(1)2(1)2 (cell dimensions: a = 62.0 A, b = 76.9 A, c = 87.5 A) with one molecule in the asymmetric unit. The structure was solved by Patterson search methods using Bacillus stearothermophilus PGK as a search model and was refined to a crystallographic R factor of 22.0%. Compared to the enzyme from B. stearothermophilus, horse, pig and yeast, the Thermotoga enzyme exhibits a drastically reduced interdomain angle, similar to the one reported for PGK from Trypanosoma brucei. Here we present crystallographic data of the first high-resolution structure of a PGK in largely closed conformation, complexed with the two products of the catalyzed reaction, and, at the same time, the first PGK structure from a hyperthermophilic organism.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Auerbach
authors.forename: U
authors.initials: U
authors.lastname: Jacob
authors.forename: M
authors.initials: M
authors.lastname: Grättinger
authors.forename: H
authors.initials: H
authors.lastname: Schurig
authors.forename: R
authors.initials: R
authors.lastname: Jaenicke
mesh_terms: D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology*; D010735:Phosphoglycerate Kinase / Q000096:biosynthesis / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D010735:Phosphoglycerate Kinase