title: In vitro formation of crocetin glucosyl esters by Crocus sativus callus extract.
abstract: Callus extracts of Crocus sativus exhibited the ability to transform all-trans-crocetin into its related glycosides between pH 7.0 and 7.6 in the presence of uridine-diphospho-glucose. The reactions involved the step-wise addition of a glucose moiety to a free carboxyl function and the 1-->6 addition of a glucose moiety to a glucosyl ester function. The kinetics of synthesis for each glycoside seemed to indicate that two distinct glucosyl transferases were implicated in the synthesis of crocin, all-trans-crocetin di-(beta-D-gentiobiosyl) ester.
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada, St-Hyacinthe, Québec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Dufresne
authors.forename: F
authors.initials: F
authors.lastname: Cormier
authors.forename: S
authors.initials: S
authors.lastname: Dorion
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology*; D004952:Esters; D006027:Glycosides / Q000737:chemistry; D014801:Vitamin A / Q000031:analogs & derivatives
chemical_list: D016588:Anticarcinogenic Agents; D000975:Antioxidants; D004952:Esters; D006027:Glycosides; C487773:trans-sodium crocetinate; D014801:Vitamin A; D002338:Carotenoids