title: Rates of spontaneous mutation in an archaeon from geothermal environments.
abstract: To estimate the efficacy of mechanisms which may prevent or repair thermal damage to DNA in thermophilic archaea, a quantitative assay of forward mutation at extremely high temperature was developed for Sulfolobus acidocaldarius, based on the selection of pyrimidine-requiring mutants resistant to 5-fluoro-orotic acid. Maximum-likelihood analysis of spontaneous mutant distributions in wild-type cultures yielded maximal estimates of (2.8 +/- 0.7) x 10(-7) and (1.5 +/- 0.6) x 10(-7) mutational events per cell per division cycle for the pyrE and pyrF loci, respectively. To our knowledge, these results provide the first accurate measurement of the genetic fidelity maintained by archaea that populate geothermal environments. The measured rates of forward mutation at the pyrE and pyrF loci in S. acidocaldarius are close to corresponding rates reported for protein-encoding genes of Escherichia coli. The normal rate of spontaneous mutation in E. coli at 37 degrees C is known to require the functioning of several enzyme systems that repair spontaneous damage in DNA. Our results provide indirect evidence that S. acidocaldarius has cellular mechanisms, as yet unidentified, which effectively compensate for the higher chemical instability of DNA at the temperatures and pHs that prevail within growing Sulfolobus cells.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, Ohio 45221-0006, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Jacobs
authors.forename: D W
authors.initials: DW
authors.lastname: Grogan
mesh_terms: D004249:DNA Damage; D006358:Hot Temperature; D009154:Mutation / Q000187:drug effects; D009963:Orotic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D016945:Sulfolobus acidocaldarius / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
chemical_list: D009963:Orotic Acid; C001242:5-fluoroorotic acid