Effect of pentoxifylline on veno-occlusive priapism-induced corporeal tissue lipid peroxidation in a rat model.
OBJECTIVE
To investigate whether pentoxifylline could play a role in attenuation of the hazardous effects of ischemia/reperfusion on corporeal tissue in a rat model of veno-occlusive priapism (VOP).


METHODS
Placebo and pentoxifylline were given to eight groups of rats prior to priapism being induced by a vacuum constrictive device for durations of 6 and 12 h, respectively. Half of the groups of rats that underwent the same duration of priapism (ischemic) were subjected to 1 h of detumescence after band removal (reperfusion). One group underwent no manipulation and no drug administration and served as a baseline determination (control). Corporeal homogenates were examined for lipid peroxidation (LP) derived malondialdehyde (MDA) accumulation via thiobarbituric acid assay.


RESULTS
MDA concentration differed significantly between VOP rats and controls (P < 0.001) but did not differ significantly between ischemic-only groups and reperfused groups (P < 0.05). In the pentoxifylline-pretreated groups, although MDA accumulation tended to be slightly lower than in the placebo groups, the difference was not statistically significant (P < 0.05) either in the 6- or 12-h duration priapic groups.


CONCLUSIONS
LP, an indicator of radical oxygen metabolite (ROM) induced injury, occurs in rat corporeal tissue during and after abolishment of VOP. Single-dose pentoxifylline pretreatment failed to exert a protective effect on corporeal tissue in a rat model of VOP in terms of attenuation of LP.
Evliyaoğlu
Y
Y
Department of Urology, Adana Numune Hospital, Turkey.
Kayrin
L
L
Kaya
B
B
D000818:Animals; D004195:Disease Models, Animal; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010410:Penile Erection / Q000187:drug effects / Q000502:physiology; D010413:Penis / Q000098:blood supply / Q000293:injuries / Q000378:metabolism; D010431:Pentoxifylline / Q000494:pharmacology*; D011317:Priapism / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control
D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D010431:Pentoxifylline