title: Sodium/calcium exchanger in rat olfactory neurons.
abstract: The chemo-electrical transduction process in olfactory neurons is accompanied by a rapid and transient increase in intracellular calcium concentrations. The notion that Na+/Ca2+ exchanger activities may play a major role in extruding calcium ions out of the cell and maintaining Ca2+ homeostasis in olfactory receptor cells was assessed by means of laser scanning confocal microscopy in combination with the fluorescent indicators Fluo-3 and Fura-Red. The data indicate that high exchanger activity, which was inhibited by amiloride derivatives, is located in the dendritic knob and probably in the olfactory cilia. This result was supported by experiments using specific antiserum raised against retinal Na+/Ca2+ exchanger protein which labelled an immunoreactive protein of 230 kDa in Western blots from olfactory tissue and strongly stained the ciliary layer of the olfactory epithelium.
authors.affiliation: University Stuttgart-Hohenheim, Institute of Zoophysiology, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Noé
authors.forename: E
authors.initials: E
authors.lastname: Tareilus
authors.forename: I
authors.initials: I
authors.lastname: Boekhoff
authors.forename: H
authors.initials: H
authors.lastname: Breer
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005456:Fluorescent Dyes; D006706:Homeostasis; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019831:Sodium-Calcium Exchanger
chemical_list: D002352:Carrier Proteins; D005456:Fluorescent Dyes; D019831:Sodium-Calcium Exchanger; D002118:Calcium