Production of consistent crush lesions of murine skeletal muscle in vivo using an electromechanical device.
The crush model of injury in skeletal muscle is widely used in the investigation of tissue degeneration and regeneration. Previously, such trauma has been induced by using forceps to crush the muscle, commonly applying sufficient pressure to bring the mid-arms of the forceps together. This report introduces a reliable electromechanical device designed to generate reproducible focal lesions in skeletal muscle of mice. The tibialis anterior was crushed in 17 young adult mice. Two days after injury, the muscles were examined microscopically. By morphometric analysis, it was determined that the volumes of the lesions produced were similar (mean 0.499 mm3 +/- 0.098, range 0.278 - 0.601 mm3), and that the full extent of the damaged muscle was easily distinguished and readily quantifiable. This will allow a more precise comparison in future investigations into regenerative differences between age groups, satellite cell activation and the inflammatory response.
Rushton
J L
JL
School of Biological Sciences, University of Manchester, UK.
Davies
I
I
Horan
M A
MA
Mahon
M
M
Williams
R
R
D000818:Animals; D006614:Hindlimb; D051379:Mice; D008815:Mice, Inbred Strains; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000293:injuries* / Q000473:pathology; D012107:Research Design