title: Accuracy of cholesterol measurements in Italian clinical laboratories. Joint project GISSI prevention--Italian Society of Clinical Biochemistry. SIBioC GISSI Prevenzione Group.
abstract: We report the results of an external quality assessment scheme for serum total cholesterol measurement involving about 100 Italian laboratories participating in an epidemiological study of post myocardial infarction. Two frozen human serum pools with Abell-Kendall assigned values are distributed quarterly at the laboratories (up to now seven events occurred); the obtained results are evaluated and discussed. In one exercise (# 5) duplicated measurements were repeated on three different days. Eighty-five to 98% of the laboratories obtained results within the total error limits (+/- 8.9%). But, while precision (calculated on the six replicates of exercise # 5) is good (90% of the laboratories obtained CV < 3%), inaccuracy problems are evident in every event. Indeed the mean bias from the reference method value ranged from 1.54 and 3.49% in the various events.
authors.affiliation: Laboratorio di Chimica Clinica ed Ematologia, Ospedale Civile Maggiore, Verona, Italy.
authors.forename: M S
authors.initials: MS
authors.lastname: Graziani
authors.forename: F
authors.initials: F
authors.lastname: Ceriotti
authors.forename: A
authors.initials: A
authors.lastname: Carobene
authors.forename: A
authors.initials: A
authors.lastname: Modenese
authors.forename: C
authors.initials: C
authors.lastname: Ferrero
authors.forename: E
authors.initials: E
authors.lastname: De Giorgi
authors.forename: M
authors.initials: M
authors.lastname: Franzin
authors.forename: C
authors.initials: C
authors.lastname: Marchetti
authors.forename: P
authors.initials: P
authors.lastname: Brambilla
authors.forename: M
authors.initials: M
authors.lastname: Bertona
authors.forename: D
authors.initials: D
authors.lastname: Giannone
authors.forename: G
authors.initials: G
authors.lastname: Righetti
authors.forename: A
authors.initials: A
authors.lastname: Marocchi
authors.forename: L
authors.initials: L
authors.lastname: Prencipe
authors.forename: C
authors.initials: C
authors.lastname: Franzini
authors.forename: P
authors.initials: P
authors.lastname: Luraschi
authors.forename: P
authors.initials: P
authors.lastname: Mocarelli
mesh_terms: D000704:Analysis of Variance; D015982:Bias; D002624:Chemistry, Clinical / Q000592:standards* / Q000706:statistics & numerical data; D002784:Cholesterol / Q000097:blood* / Q000592:standards; D003327:Coronary Disease / Q000097:blood / Q000453:epidemiology / Q000517:prevention & control; D006801:Humans; D007558:Italy / Q000453:epidemiology; D007753:Laboratories / Q000592:standards / Q000706:statistics & numerical data; D009203:Myocardial Infarction / Q000097:blood; D011786:Quality Control; D012958:Societies, Scientific; D013997:Time Factors
chemical_list: D002784:Cholesterol