Factors influencing the antioxidant activity determined by the ABTS.+ radical cation assay.
This study introduces a simple direct antioxidant assay, based on the reduction of the ABTS.+ radical cation, and compares it with the myoglobin/ABTS.+ assay. The methods give closely similar results, establishing that the antioxidants studied to date in the latter assay act by scavenging the ABTS.+ radical cation and not by inhibiting its formation through reduction of ferryl myoglobin or reaction with H2O2.
Miller
N J
NJ
International Antioxidant Research Centre, UMDS-Guy's Hospital, London, UK.
Rice-Evans
C A
CA
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism; D052160:Benzothiazoles; D003124:Colorimetry / Q000379:methods; D016166:Free Radical Scavengers; D005609:Free Radicals / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide; D009211:Myoglobin / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D013451:Sulfonic Acids / Q000737:chemistry / Q000378:metabolism*; D014527:Uric Acid / Q000737:chemistry / Q000378:metabolism; D014810:Vitamin E / Q000737:chemistry / Q000378:metabolism
D000975:Antioxidants; D052160:Benzothiazoles; D016166:Free Radical Scavengers; D005609:Free Radicals; D009211:Myoglobin; D013451:Sulfonic Acids; D014810:Vitamin E; D014527:Uric Acid; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid