A comparison of the germline potential of differently aged ES cell lines and their transfected descendants.
The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gene trap experiments were tested for their ability to colonize the germline. The maximal g.l.t. age for wild-type ES cells was equal to passage 26 and for transfected clones was equivalent to passage 32 of parental lines. It is shown that wild-type ES cells of less than a passage 15 should be used for effective production of transgenic g.l.t. clones. A simple system is outlined to evaluate the probability of g.l.t. on the basis of the chimaeric progeny obtained.
Fedorov
L M
LM
Max-Planck Institut für Immunbiologie, Freiburg, Germany.
Haegel-Kronenberger
H
H
Hirchenhain
J
J
D000367:Age Factors; D054366:Agouti Signaling Protein; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002678:Chimera / Q000235:genetics; D005260:Female; D005854:Germ Cells; D006200:Hair Color / Q000235:genetics; D007246:Infertility / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic / Q000235:genetics*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D017678:Sex Distribution; D013234:Stem Cells / Q000502:physiology*; D014162:Transfection
D054366:Agouti Signaling Protein; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins