In situ interleukin 5 gene expression in pediatric Crohn's disease.
BACKGROUND
Eosinophils contribute to the intestinal inflammatory infiltrate in Crohn's disease (CD). Eosinophilic infiltration occurs early in Crohn's recurrences, and a release of eosinophil cationic proteins has been observed in active CD. The proliferation, differentiation, and activation of eosinophils are highly dependent on the cytokine interleukin 5 (IL5). In the present study, we used in situ hybridization (ISH) to investigate the expression of the IL5 gene in intestinal specimens from patients with CD.


METHODS
We studied 14 intestinal samples from eight children who had undergone ileocolectomy for advanced CD. The samples were examined for the intensity of the inflammatory infiltrate. Normal pediatric intestine specimens served as controls. In situ hybridization was performed on frozen tissue using radiolabeled IL5 mRNA probes.


RESULTS
Positive signal with the IL5 antisense probe was observed within numerous cells infiltrating the specimens involved with CD. The number of IL5-expressing cells correlated with the histological grade of inflammation. Most of the labeled cells were eosinophils, characterized by their bilobed nuclei. Rare IL5-positive cells were detected in the control tissues. No positive signal was obtained with the IL5 sense probe.


CONCLUSIONS
These results suggest that IL5 can be produced by eosinophils at the sites of inflammation in active CD and could be involved in the immune response by activating eosinophils, at least in part through an autocrine pathway, and perhaps by interacting with B and T cells.
Hankard
G F
GF
Service d'Anatomie Pathologique, Hôpital Robert Debré, Paris, France.
Brousse
N
N
Cézard
J P
JP
Emilie
D
D
Peuchmaur
M
M
D000293:Adolescent; D002648:Child; D003106:Colon / Q000737:chemistry / Q000276:immunology / Q000473:pathology*; D003424:Crohn Disease / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D007082:Ileum / Q000737:chemistry / Q000276:immunology / Q000473:pathology*; D017403:In Situ Hybridization; D015848:Interleukin-5 / Q000096:biosynthesis / Q000235:genetics*; D007413:Intestinal Mucosa / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis
D015848:Interleukin-5; D012333:RNA, Messenger