Cloning and sequencing of a species-specific nucleotide fragment of Borrelia burgdorferi sensu stricto, which is repeated in several plasmids of the species.
Among the etiological agents of Lyme disease, Borrelia burgdorferi sensu stricto strains carry a 16 kb plasmid, which did not hybridize to plasmids of B. garinii and B. afzelii strains. A 1271 bp DNA fragment of the 16 kb plasmid was cloned. It hybridized to several plasmids of this species (16, 27 and 55 kb). Sequencing of the cloned insert revealed a 327 bp ORF coding for a 14 kDa protein of unknown function, which could be expressed in E. coli. This ORF, conserved among B. burgdorferi sensu stricto strains, was carried by the same three plasmids.
Misonne
M C
MC
Microbial Pathogenesis Unit, University of Louvain Medical School, Brussels, Belgium.
Schuttler
M
M
Dernelle
J M
JM
De Kesel
M
M
Hoet
P P
PP
D000595:Amino Acid Sequence; D001483:Base Sequence; D025065:Borrelia burgdorferi; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins