title: An STS-based radiation hybrid map of the human genome.
abstract: We have constructed a physical map of the human genome by using a panel of 83 whole genome radiation hybrids (the Stanford G3 panel) in conjunction with 10,478 sequence-tagged sites (STSs) derived from random genomic DNA sequences, previously mapped genetic markers, and expressed sequences. Of these STSs, 5049 are framework markers that fall into 1766 high-confidence bins. An additional 945 STSs are indistinguishable in their map location from one or more of the framework markers. These 5994 mapped STSs have an average spacing of 500 kb. An additional 4484 STSs are positioned with respect to the framework markers. Comparison of the orders of markers on this map with orders derived from independent meiotic and YAC STS-content maps indicates that the error rate in defining high-confidence bins is < 5%. Analysis of 322 random cDNAs indicates that the map covers the vast majority of the human genome. This STS-based radiation hybrid map of the human genome brings us one step closer to the goal of a physical map containing 30,000 unique ordered landmarks with an average marker spacing of 100 kb.
authors.affiliation: Stanford Human Genome Center, Palo Alto, California, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Stewart
authors.forename: K B
authors.initials: KB
authors.lastname: McKusick
authors.forename: A
authors.initials: A
authors.lastname: Aggarwal
authors.forename: E
authors.initials: E
authors.lastname: Bajorek
authors.forename: S
authors.initials: S
authors.lastname: Brady
authors.forename: A
authors.initials: A
authors.lastname: Chu
authors.forename: N
authors.initials: N
authors.lastname: Fang
authors.forename: D
authors.initials: D
authors.lastname: Hadley
authors.forename: M
authors.initials: M
authors.lastname: Harris
authors.forename: S
authors.initials: S
authors.lastname: Hussain
authors.forename: R
authors.initials: R
authors.lastname: Lee
authors.forename: A
authors.initials: A
authors.lastname: Maratukulam
authors.forename: K
authors.initials: K
authors.lastname: O'Connor
authors.forename: S
authors.initials: S
authors.lastname: Perkins
authors.forename: M
authors.initials: M
authors.lastname: Piercy
authors.forename: F
authors.initials: F
authors.lastname: Qin
authors.forename: T
authors.initials: T
authors.lastname: Reif
authors.forename: C
authors.initials: C
authors.lastname: Sanders
authors.forename: X
authors.initials: X
authors.lastname: She
authors.forename: W L
authors.initials: WL
authors.lastname: Sun
authors.forename: P
authors.initials: P
authors.lastname: Tabar
authors.forename: S
authors.initials: S
authors.lastname: Voyticky
authors.forename: S
authors.initials: S
authors.lastname: Cowles
authors.forename: J B
authors.initials: JB
authors.lastname: Fan
authors.forename: C
authors.initials: C
authors.lastname: Mader
authors.forename: J
authors.initials: J
authors.lastname: Quackenbush
authors.forename: R M
authors.initials: RM
authors.lastname: Myers
authors.forename: D R
authors.initials: DR
authors.lastname: Cox
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human / Q000235:genetics* / Q000528:radiation effects; D006224:Cricetinae; D004171:Diploidy; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects*; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D016324:Sequence Tagged Sites; D012984:Software
chemical_list: D005819:Genetic Markers