Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.
A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.
Monteiro
L
L
Laboratoire de Bactériologie, Université de Bordeaux 2, France. helico@labhel.u-bordeaux2.fr
Bonnemaison
D
D
Vekris
A
A
Petry
K G
KG
Bonnet
J
J
Vidal
R
R
Cabrita
J
J
Mégraud
F
F
D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011134:Polysaccharides
D011134:Polysaccharides