title: Influence of two diets on pharmacokinetic parameters of allopurinol and oxypurinol in healthy beagles.
abstract: OBJECTIVE
  To determine whether diet influences the metabolism of IV administered allopurinol in healthy dogs.
  
  
  METHODS
  6 healthy female Beagles, 4.9 to 5.2 years old and weighing 9.6 to 11.5 kg.
  
  
  METHODS
  Allopurinol was administered IV (10 mg/kg) while dogs consumed a 10.4% protein (dry weight), casein-based diet or a 31.4% (dry weight), meat-based diet. After each dose, plasma samples were obtained at timed intervals, and concentrations of allopurinol and its active metabolite, oxypurinol, were determined by high-performance liquid chromatography. An iterative, nonlinear regression analytical program was used to determine the weighted least-squares, best-fit curves for plasma allopurinol and oxypurinol concentration-time data. From these data, pharmacokinetic parameters were calculated.
  
  
  RESULTS
  Pharmacokinetic parameters for allopurinol and oxypurinol were not different when comparing the effect of diet.
  
  
  CONCLUSIONS
  There is no influence of diet on pharmacokinetic parameters of allopurinol or oxypurinol.
  
  
  CONCLUSIONS
  In contrast to observations in human beings, allopurinol metabolism is not influenced by diet. Therefore, formation of xanthine-containing calculi in dogs consuming a high-protein diet and receiving allopurinol is probably not attributable to alteration of allopurinol metabolism.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Bartges
authors.forename: C A
authors.initials: CA
authors.lastname: Osborne
authors.forename: L J
authors.initials: LJ
authors.lastname: Felice
authors.forename: L A
authors.initials: LA
authors.lastname: Koehler
authors.forename: L K
authors.initials: LK
authors.lastname: Ulrich
authors.forename: K A
authors.initials: KA
authors.lastname: Bird
authors.forename: M
authors.initials: M
authors.lastname: Chen
mesh_terms: D000493:Allopurinol / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary; D003404:Creatinine / Q000652:urine; D018592:Cross-Over Studies; D004032:Diet / Q000662:veterinary*; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs / Q000097:blood / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D005260:Female; D018565:Food-Drug Interactions; D007275:Injections, Intravenous; D008903:Minerals / Q000032:analysis; D010117:Oxypurinol / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D013997:Time Factors
chemical_list: D004044:Dietary Proteins; D004791:Enzyme Inhibitors; D008903:Minerals; D000493:Allopurinol; D003404:Creatinine; D010117:Oxypurinol