title: Structure of the ascidian, Halocynthia roretzi, troponin C gene.
abstract: Two distinct cDNAs encoding troponin C (TnC) isoforms were isolated from the ascidian, Halocynthia roretzi. One is expressed in adult body wall smooth muscle and heart muscle, and the other in larval striated muscle. The H. rorezti gene is composed of 7 exons separated by 6 introns, and Southern blot analysis showed that TnC is a single copy gene product. The two isoforms of TnC were derived through the alternative splicing of the third exon.
authors.affiliation: Biological Institute, Graduate School of Science, Tohoku University, Sendai, Miyagi.
authors.forename: H J
authors.initials: HJ
authors.lastname: Yuasa
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Takagi
mesh_terms: D000367:Age Factors; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D019209:Troponin C / Q000235:genetics* / Q000378:metabolism; D014561:Urochordata / Q000235:genetics* / Q000254:growth & development; D014714:Vertebrates / Q000235:genetics
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D019209:Troponin C