Inhibition by protein kinase C inhibitor of expression of leukocyte function-associated antigen-1 molecules in rat hepatoma AH66F cells.
To examine the mechanism of inhibition by protein kinase C (PKC) inhibitors of the adhesion of highly malignant hepatoma AH66F cells to the mesentery-derived mesothelial cell (M-cell) layer through leukocyte function-associated antigen-1 (LFA-1)/intercellular adhesion molecule-1, the effects of a PKC inhibitor, NA-382, on the expression of LFA-1 molecules in AH66F cells were examined and compared with those in thymocytes from normal rats. NA-382 inhibited the adhesion of AH66F cells to the M-cell layer and the expression of LFA-1 on the membrane of the hepatoma cells after treatment for more than 24 h. It was confirmed that AH66F cells express similar mRNAs for LFA-1 subunits to those of thymocytes, and their levels were also decreased after treatment with NA-382. On the other hand, the LFA-1 mediated adhesion and the expression of both protein and mRNA for LFA-1 subunits in thymocytes were not changed by the PKC inhibitor. These results suggest that the expression of LFA-1 molecules in AH66F cells may be regulated by PKC via quite different mechanisms from those in normal lymphocytes.
Nomura
M
M
Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa.
Sugiura
N
N
Moritani
S
S
Miyamoto
K
K
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D017931:DNA Primers; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005434:Flow Cytometry; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000503:physiopathology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis*; D046911:Macromolecular Substances; D008643:Mesentery / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats; D019311:Staurosporine / Q000031:analogs & derivatives; D013601:T-Lymphocytes / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D017931:DNA Primers; D004791:Enzyme Inhibitors; D016169:Lymphocyte Function-Associated Antigen-1; D046911:Macromolecular Substances; C075317:N-ethoxycarbonyl-7-oxostaurosporine; D011493:Protein Kinase C; D019311:Staurosporine