Effects of fraxetin on glutathione redox status.
We have evaluated the effects of an oral treatment of mice with fraxetin (25 mg/kg for 30 days) on the glutathione system (GSH, GSSG, and GSSG/GSH ratio as stress index), glutathione reductase (GR) and glutathione peroxidase (GPx) in liver supernatants from male C57BL/6J mice (18-month old). A significant antioxidant effect in vivo was found under this treatment by a decrease in the GSSG/GSH ratio and an increased activity of GR compared with the control mice. GSSG rate and GSSG/GSH ratio were correlated with the decline of GPx++ activity. Our results of increased GR activity could be considered as a supercompensation in glutathione redox status that involves a decrease in the accumulation of GSSG, as well as, in GSSG/GSH ratio. Finally, we suggest that this possible mechanism of supercompensation could lead to an enhancement in the average life span.
Martín-Aragón
S
S
Pharmacology Department, Complutense University, Madrid, Spain.
Benedí
J M
JM
Villar
A M
AM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003374:Coumarins / Q000494:pharmacology*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism*; D019803:Glutathione Disulfide; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction
D000975:Antioxidants; D003374:Coumarins; C105671:fraxetin; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; D019803:Glutathione Disulfide