title: Mg2+- or Mn2+-dependent endonuclease activities of human myeloid leukemia cells capable of producing nucleosomal-size DNA fragmentation.
abstract: The presence of at least two distinct Mg2+- or Mn2+-dependent, Ca2+-independent endonuclease activities was shown in the myeloid leukemia cell line P39. One of them was recovered from nuclear extracts and the other from a cytoplasmic fraction. The molecular size of the former was 30 kDa in both gel filtration and activity gel and that of the latter approximately 130-140 kDa in gel filtration and 65-70 kDa in activity gel. These two activities were almost completely inhibited by 0.1 mM ZnCl2 or 0.1 mM aurintricarboxylic acid, common inhibitors of apoptosis. Both could produce nucleosomal-size DNA fragmentation when incubated with diethyl-pyrocarbonate-treated nuclei as substrates, and the pattern of cleavage was 3'-OH and 5'-P. Taken together, either or both of these activities may be associated with apoptosis of myeloid leukemia cells.
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kawabata
authors.forename: N
authors.initials: N
authors.lastname: Anzai
authors.forename: H
authors.initials: H
authors.lastname: Masutani
authors.forename: T
authors.initials: T
authors.lastname: Hirama
authors.forename: T
authors.initials: T
authors.lastname: Hishita
authors.forename: M
authors.initials: M
authors.lastname: Dodo
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Okuma
mesh_terms: D002850:Chromatography, Gel; D053938:DNA Fragmentation; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D007951:Leukemia, Myeloid / Q000201:enzymology* / Q000473:pathology; D008274:Magnesium / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*
chemical_list: D009707:Nucleosomes; D008345:Manganese; D004720:Endonucleases; D008274:Magnesium