[Phenotype of peripheral blood lymphocytes of healthy donors and patients with corneal allotransplantation under conditions of hyperbaric oxygenation].
The purpose of this study was to elucidate the possible immunomodulating effect of hyperbaric oxygenation (HBO) on the immune status of patients with various ophthalmopathies and developing the graft-versus-host reaction after perforating keratoplasty. Surface phenotype of the peripheral blood lymphocytes was studied by two-color flow cytofluorometry in donors and patients with the rejection syndrome after transplantation of the cornea before and after a course of oxygen therapy. HBO exerted no immunodepressive effect, as judged from changes in the subpopulation composition (CD3+, CD4+, CD8+, CD16+, and CD56+) and expression of activation markers (CD25 and HLA-DR), in both patients and donors. The most probable explanation is that HBO effect on the immune system is indirect. On the other hand, from a clinical viewpoint the effect of HBO is undoubtedly positive, and hence, HBO exposure may be recommended for clinical application in transplantations of the cornea.
Krasnov
M M
MM
Kasparov
A A
AA
Pirtskhlava
M A
MA
Koval'chuk
L V
LV
Pavliuk
A S
AS
Veselova
A V
AV
Mordvinova
L N
LN
D000328:Adult; D000368:Aged; D005260:Female; D005434:Flow Cytometry; D006087:Graft vs Host Reaction; D006801:Humans; D006931:Hyperbaric Oxygenation; D007231:Infant, Newborn; D015948:Keratoplasty, Penetrating; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014019:Tissue Donors
Fenotip limfotsitov perifericheskoĭ krovi zdorovykh donorov i bol'nykh s rogovichnym allotransplantatom v usloviiakh giperbaricheskoĭ oksigenatsii.