Laser Doppler perfusion imaging: new technique for determination of perfusion and reperfusion of splanchnic organs and tumor tissue.
OBJECTIVE
Several investigations indicated that laser Doppler flowmetry on the liver surface reflects relative changes of the total liver blood flow. In this study, Laser Doppler Perfusion Imaging (LDI), monitoring the surface only, was used for measurements of tissue perfusion of normal and/or impaired liver, pancreas, spleen, stomach and intestines, and the blood flow of hepatic tumors in rats.


METHODS
Eighty Wistar/Furth rats were divided into five groups. Group I served as controls. Groups II and III underwent ischemic injury of the liver and intestine with or without the administration of WEB2170, a platelet-activating factor receptor antagonist. Laser-induced photodynamic therapy (PDT) utilizing delta-amino levulinic acid sensitization was performed in Groups IV and V.


RESULTS
Normal pancreas and intestine had a high LDI perfusion value and the liver and stomach exhibited a medium perfusion value whereas the perfusion value from the spleen was low. WEB2170 improved the reperfusion of the postischemic liver and intestine. An immediate decrease in surface blood flow of hepatic tissue treated by laser-induced PDT and a decreased blood flow in large tumors were observed.


CONCLUSIONS
LDI is a useful technique for the measurement of tissue perfusion of various splanchnic organs or tumor tissues.
Liu
D L
DL
Department of Oncology, Lund University Hospital, Sweden.
Svanberg
K
K
Wang
I
I
Andersson-Engels
S
S
Svanberg
S
S
D000622:Aminolevulinic Acid / Q000627:therapeutic use; D000818:Animals; D001381:Azepines; D007422:Intestines / Q000098:blood supply; D007511:Ischemia / Q000503:physiopathology*; D017078:Laser-Doppler Flowmetry; D008099:Liver / Q000098:blood supply; D008113:Liver Neoplasms / Q000098:blood supply* / Q000188:drug therapy; D010179:Pancreas / Q000098:blood supply; D010778:Photochemotherapy; D010975:Platelet Aggregation Inhibitors; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D013152:Splanchnic Circulation; D013154:Spleen / Q000098:blood supply; D013270:Stomach / Q000098:blood supply; D014230:Triazoles
D001381:Azepines; D010975:Platelet Aggregation Inhibitors; D014230:Triazoles; D000622:Aminolevulinic Acid; C063964:bepafant