Selective expression of HSP70-1 gene in the adrenal cortex but not in the medulla of thermally stressed rats.
The heat shock protein 70 (HSP70) gene expression in rat adrenal glands was investigated. In situ hybridization and Northern blotting revealed a marked increase of inducible HSP70 (HSP70-1) gene expression in the adrenal cortex, but not in the medulla, after whole body hyperthermia. In vivo footprinting assay of HSP70-1 promoter region suggested a possible involvement of other factor(s) in addition to the heat shock factor (HSF). We also detected significant increases of both ACTH and corticosterone in the plasma of the hyperthermic rats. These results suggested some physiological factors other than HSF may also participate in the tissue-specific induction of HSP70-1 gene in vivo.
Shimizu
K
K
Department of Biochemistry, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Nomoto
M
M
Ueta
Y
Y
Konishi
T
T
Abe
T
T
Gotoh
S
S
Suzuki
K
K
Nakamura
T
T
Higashi
K
K
D000302:Adrenal Cortex / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D003345:Corticosterone / Q000097:blood; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D011401:Promoter Regions, Genetic; D015800:Protozoan Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013312:Stress, Physiological / Q000378:metabolism*
D018840:HSP70 Heat-Shock Proteins; C490435:Hspa2 protein, rat; D015800:Protozoan Proteins; D012333:RNA, Messenger; C081884:heat-shock protein 70.1; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone