title: SNAP25 and GAP-43 behave differently in decentralized rat superior cervical ganglia.
abstract: The behaviour of synaptosome associated protein of mol. wt 25 kDa (SNAP25) in decentralized rat superior cervical ganglia (SCG) was investigated in order to observe its possible involvement in adrenergic postganglionic neuronal plasticity. Immunofluorescence and immunoblot results showed that the protein was increased in the nerve terminals in irides 8 days after operation. The intra-ganglionic nerve terminals and nerve fibres differed in their content of GAP-43 and SNAP25: GAP-43, which could not be observed at 1 day, appeared at 3 days after cutting the cervical sympathetic trunk, whilst SNAP25-immunoreactive material was still undetectable at this time. Immunoblot data also showed that SNAP25 did not reach control levels at 3 and 8 days after decentralization, in contrast to GAP-43. This observation may imply that SNAP25 is rapidly transported to its functional destinations immediately after synthesis and is possibly mainly involved in the remodelling of long projection pathways.
authors.affiliation: Department of Anatomy and Cell Biology, Göteborg University, Sweden.
authors.forename: X E
authors.initials: XE
authors.lastname: Hou
authors.forename: K
authors.initials: K
authors.lastname: Lundmark
authors.forename: A
authors.initials: A
authors.lastname: Dahlstrom
mesh_terms: D000818:Animals; D003714:Denervation; D005260:Female; D005455:Fluorescent Antibody Technique; D019922:GAP-43 Protein; D015151:Immunoblotting; D007150:Immunohistochemistry; D007498:Iris; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D008565:Membrane Proteins; D009411:Nerve Endings / Q000502:physiology / Q000648:ultrastructure; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000378:metabolism; D009473:Neuronal Plasticity; D051381:Rats; D017207:Rats, Sprague-Dawley; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000502:physiology*; D050825:Synaptosomal-Associated Protein 25
chemical_list: D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25