title: Possible involvement of nonbilayer lipids in the stimulation of the activity of cytochrome P450SCC (CYP11A1) and its propensity to induce vesicle aggregation.
abstract: Phosphatidylcholines with saturated branched fatty acyl chains substituted in the 2-positions of the main chains have a significant stimulating effect on both the side chain cleavage activity of cytochrome P450SCC and the ability of P450SCC to induce a specific vesicle aggregation in lipid-vesicle reconstituted systems. Stimulation increases with increasing content of the branched phosphatidylcholines and is sensitive to its acyl chain structure and composition. Because branched phosphatidylcholines containing 2-alkyl substituted fatty acid chains belong to a new class of phosphatidylcholines forming inverted nonbilayer phases the results indicate that nonbilayer lipids might play an important role in the function of mitochondrial P450SCC.
authors.affiliation: Max Delbruck Centrum for Molecular Medidine, Berlin-Buch, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Schwarz
authors.forename: P
authors.initials: P
authors.lastname: Kisselev
authors.forename: R
authors.initials: R
authors.lastname: Wessel
authors.forename: S
authors.initials: S
authors.lastname: Pisch
authors.forename: U
authors.initials: U
authors.lastname: Bornscheuer
authors.forename: R D
authors.initials: RD
authors.lastname: Schmid
mesh_terms: D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism*; D004134:Dimyristoylphosphatidylcholine; D008081:Liposomes / Q000378:metabolism*; D009391:Nephelometry and Turbidimetry; D010713:Phosphatidylcholines / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D002786:Cholesterol Side-Chain Cleavage Enzyme; C017251:1,2-oleoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine