Effect of xylitol and trehalose on dry resistance of yeasts.
The effects of dehydration/rehydration on two strains of Saccharomyces cerevisiae: S600, a metabolically engineered xylose-utilising strain, and H158, the non-xylose-utilising host strain; and on the naturally xylose-utilising yeast Pachysolen tannophilus CBS 4044, were compared after glucose and xylose utilisation respectively. The yeast strains differed in their ability to excrete and accumulate intracellular xylitol. A high intracellular xylitol content before and after dehydration coincided with a higher viability after a dehydration/ rehydration cycle. The intracellular trehalose content increased during dehydration in all three yeast strains, but this did not correspond to enhanced cell viability after dehydration/rehydration. The results are discussed in relation to the ability of xylitol and trehalose to structure water.
Krallish
I
I
Institute of Microbiology and Biotechnology, Latvian University, Riga, Latvia.
Jeppsson
H
H
Rapoport
A
A
Hahn-Hägerdal
B
B
D019342:Acetic Acid / Q000032:analysis; D003890:Desiccation; D000431:Ethanol / Q000032:analysis; D005285:Fermentation; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D005990:Glycerol / Q000032:analysis; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014199:Trehalose / Q000032:analysis / Q000378:metabolism*; D014867:Water / Q000378:metabolism*; D014993:Xylitol / Q000032:analysis / Q000378:metabolism*; D014994:Xylose / Q000032:analysis; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism*
D014867:Water; D000431:Ethanol; D014994:Xylose; D014199:Trehalose; D005947:Glucose; D005990:Glycerol; D019342:Acetic Acid; D014993:Xylitol