title: Mapping in pig of genes involved in sexual differentiation: AMH, WT1, FTZF1, SOX2, SOX9, AHC, and placental and embryonic CYP19.
abstract: Intersexuality has been reported in pigs and investigations are in progress to identify and physically map the genes involved in sex-reversal. In this study we have mapped on porcine chromosomes seven genes which might be implicated in this developmental pathway. Four genes were mapped by radioactive in situ hybridization: AMH (Anti-Müllerian Hormone) and WT1 (Wilms' Tumor gene 1) were both mapped to pig chromosome 2 (SSC2) in the q14-->q21 and p14-->q11 regions, placental CYP19 (cytochrome P450, subfamily XIX) and FTZF1 (fushi tarazu factor [Drosophila] homolog 1, alias SF1 [steroidogenic factor 1]) to pig chromosome 1 (SSC1) in the q14-->q17 and q210-->q211 regions respectively. Four other genes were regionally located by PCR analysis on a cytogenetically characterized porcine somatic cell hybrid panel: a second CYP19 gene (expressed in embryo) was mapped to porcine 1q12-->q17, AHC (alias DAX1, adrenal hypoplasia congenital) to porcine Xp24, SOX2 and SOX9 (SRY sex determining region Y-box 2 and 9) to 13q23-->q41 and 12p13-->p11 respectively. These results are in global agreement with mapping data available in other mammalian species.
authors.affiliation: INRA, Laboratoire de Génétique Cellulaire BP27, Castanet-Tolosan, France. Lahbib@toulouse.inra.fr
authors.forename: Y
authors.initials: Y
authors.lastname: Lahbib-Mansais
authors.forename: A
authors.initials: A
authors.lastname: Barbosa
authors.forename: M
authors.initials: M
authors.lastname: Yerle
authors.forename: P
authors.initials: P
authors.lastname: Parma
authors.forename: D
authors.initials: D
authors.lastname: Milan
authors.forename: E
authors.initials: E
authors.lastname: Pailhoux
authors.forename: J
authors.initials: J
authors.lastname: Gellin
authors.forename: C
authors.initials: C
authors.lastname: Cotinot
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone; D001141:Aromatase / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D057137:DAX-1 Orphan Nuclear Receptor; D004268:DNA-Binding Proteins / Q000235:genetics; D051717:Fushi Tarazu Transcription Factors; D006023:Glycoproteins; D006131:Growth Inhibitors / Q000235:genetics; D024002:HMGB Proteins; D006609:High Mobility Group Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D006822:Hybrid Cells; D009687:Nuclear Proteins / Q000235:genetics; D010920:Placenta; D055758:SOX9 Transcription Factor; D055748:SOXB1 Transcription Factors; D012733:Sex Differentiation / Q000235:genetics*; D054339:Steroidogenic Factor 1; D013552:Swine / Q000235:genetics*; D013735:Testicular Hormones / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D025721:WT1 Proteins
chemical_list: D057137:DAX-1 Orphan Nuclear Receptor; D004268:DNA-Binding Proteins; D051717:Fushi Tarazu Transcription Factors; D006023:Glycoproteins; D006131:Growth Inhibitors; D024002:HMGB Proteins; D006609:High Mobility Group Proteins; D018398:Homeodomain Proteins; C539966:Nr0b1 protein, mouse; D009687:Nuclear Proteins; D055758:SOX9 Transcription Factor; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; C527933:Sox9 protein, mouse; D054339:Steroidogenic Factor 1; D013735:Testicular Hormones; D014157:Transcription Factors; D025721:WT1 Proteins; C515058:steroidogenic factor 1, mouse; D054304:Anti-Mullerian Hormone; D001141:Aromatase