c-Jun and cyclin D1 proteins as mediators of neuronal death after a focal ischaemic insult.
c-Jun, a transcriptional activator, as well as cyclin D1, a key regulator of the cell cycle, have been described in vitro as mediators of programmed neuronal death. After trophic factor deprivation, the activation of c-jun and cyclin D1 genes is considered as a necessary step within the cellular machinery that leads to cell death. We show here that both c-Jun and cyclin D1 proteins are present in neurones within the infarcted area after experimental cerebral ischaemia in the mouse. Since their presence was associated with DNA fragmentation revealed by the TUNEL procedure, we propose that c-Jun and cyclin D1 are involved in the process of neuronal death.
Guégan
C
C
Laboratoire de Neurosciences, Université de Caen, CNRS URA 1829, Paris, France.
Lévy
V
V
David
J P
JP
Ajchenbaum-Cymbalista
F
F
Sola
B
B
D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000473:pathology* / Q000503:physiopathology; D016923:Cell Death; D019938:Cyclin D1; D016213:Cyclins / Q000096:biosynthesis*; D053938:DNA Fragmentation; D005786:Gene Expression Regulation; D016758:Genes, jun; D007150:Immunohistochemistry / Q000379:methods; D002546:Ischemic Attack, Transient / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009474:Neurons / Q000473:pathology* / Q000502:physiology; D015513:Oncogene Proteins / Q000096:biosynthesis*; D009928:Organ Specificity; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis
D016213:Cyclins; D015513:Oncogene Proteins; D016755:Proto-Oncogene Proteins c-jun; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1