Distribution of the insertion element IS240 among Bacillus thuringiensis strains.
The presence of IS240 was investigated in 69 Bacillus thuringiensis (Bt) strains including strains from serotype H1 to H45 and additional strains with known Dipteran larvae toxicity. Restriction digests of total DNA and PCR products obtained with a single 16-bases primer corresponding to the IS240 inverted repeated sequence were hybridized with the IS240A element. The results indicate that 67% of the Bt strains tested, including all known mosquitocidal strains, possess at least one IS240-related element. PCR experiments indicate that IS240 represents a family of insertion sequences with several variants.
Rosso
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Unité des Bactéries Entomopathogènes, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
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D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D004251:DNA Transposable Elements; D004175:Diptera / Q000382:microbiology; D009032:Mosquito Control; D010572:Pest Control, Biological; D012703:Serotyping; D013045:Species Specificity
D004251:DNA Transposable Elements