Cloning and transcriptional analysis of the lipA (lipoic acid synthetase) gene from Rhizobium etli.
We report here the isolation of a Rhizobium etli gene involved in lipoic acid metabolism, the lipA gene, which complements a lipA mutant strain of Escherichia coli. A promoter region (lipAp) was mapped immediately upstream of lipA and two in vivo transcription initiation sites were identified, preceded by sequences showing some homology to the -10/-35 promoter consensus sequences. The activity of the lipAp was found not to be regulated either by the carbon source or by the addition of lipoic acid. Moreover, quantitative analysis of the lipA transcript by RNase protection assays indicated its down-regulation during entry into stationary phase.
Taté
R
R
International Institute of Genetics and Biophysics, C.N.R., Naples, Italy.
Riccio
A
A
Iaccarino
M
M
Patriarca
E J
EJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D008049:Lipase / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012231:Rhizobium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
D001426:Bacterial Proteins; C079999:LipA protein, Bacteria; D012333:RNA, Messenger; D002244:Carbon; D008049:Lipase