Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon.
The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.
Brans
A
A
Centre d'Ingénierie des Protéines, Université de Liège, Belgium.
Loriaux
A
A
Thamm
I
I
Joris
B
B
Dusart
J
J
D001483:Base Sequence; D002874:Chromosome Mapping; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
D006360:Heat-Shock Proteins; D011994:Recombinant Proteins