Characterization of an HIV-1 p24gag epitope recognized by a CD8+ cytotoxic T-cell clone.
A CD8+ cytotoxic T-cell clone that recognized HIV p24gag was isolated from an infected individual. The minimal epitope was localized to amino acids 308-316 (QASQEVKNW). Using allogeneic target cells, we found that lysis was restricted by the HLA-Cw0401 molecule. We observed that C1R cells, that express the HLA-Cw0401 allele are able to present the peptide to the cytotoxic clone, but with reduced efficiency. Other B-cell lines, that have been genotyped as HLA-Cw0401+ were unable to present the peptide to the clone, suggesting the existence of other variants of HLA-Cw0401 or a loss of cell surface expression of this molecule.
Buseyne
F
F
Unité de virologie et d'immunologie cellulaire, URA CNRS 1157, Institut Pasteur, Paris, France.
Stevanovic
S
S
Rammensee
H G
HG
Rivière
Y
Y
D000483:Alleles; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D018604:Epitope Mapping; D000939:Epitopes; D016655:HIV Core Protein p24 / Q000737:chemistry / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics / Q000276:immunology / Q000502:physiology; D009842:Oligopeptides; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism
D000939:Epitopes; D016655:HIV Core Protein p24; D015395:Histocompatibility Antigens Class I; D009842:Oligopeptides