[Comparative characteristics of microbial proteases by the level of hydrolysis of protein substrates].
Screening of enzyme preparations displaying a maximum proteolytic activity at pH 4.0-5.5 and effecting deep proteolysis of plant proteins was performed. Amyloprotooryzin prepared from Aspergillus oryzae 387 containing a complex of proteolytic enzymes was the most effective. The amino acid composition of the hydrolysates obtained was studied. Amyloprotooryzin increased the contents of amino acids by 108-227%, depending on the substrate used. The enzymatic complex of amyloprotooryzin was studied; in addition, proteases, alpha-amylase, exo-beta-glucanase, and xylanase were detected in the complex.
Rimareva
L V
LV
Overchenko
M B
MB
Serba
E M
EM
Trifonova
V V
VV
D000164:Acremonium / Q000201:enzymology*; D001230:Aspergillus / Q000201:enzymology*; D001407:Bacillus / Q000201:enzymology*; D010450:Endopeptidases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology*; D013379:Substrate Specificity
D011506:Proteins; D010450:Endopeptidases
Sravnitel'naia kharakteristika mikrobnykh proteaz po stepeni gidroliza belkovykh substratov.