Use of nucleic acid probes to identify mycobacteria directly from Difco ESP-Myco bottles.
Mycobacterial isolates were identified directly from positive ESP-Myco bottles by use of nucleic acid probes. Retrospective analysis of 360 cultures which grew either Mycobacterium tuberculosis, M. avium complex, or M. gordonae showed that 87% were identified by direct testing of an aliquot obtained at the time a positive culture was detected. Another 12% of these cultures gave results in the equivocal range, with only 1% of the isolates yielding negative results on initial testing.
Labombardi
V J
VJ
Department of Laboratories, St. Vincents Hospital and Medical Center, New York, NY 10011, USA.
Carter
L
L
Massarella
S
S
D015373:Bacterial Typing Techniques / Q000295:instrumentation; D015336:Molecular Probe Techniques; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification