Concordant expression of class II and class III CD34 epitopes on haemopoietic cells in leukapheresis and cord blood samples with CD45/CD34 dual staining.
We used flow cytometry with CD45/CD34 dual antibody labelling to investigate the relative expression of class II and class III CD34 epitopes on haemopoietic progenitor cells (HPC) in 22 leukapheresis and cord blood samples. There was a close correlation between class II (QBEnd-10) and class III (clone 581) CD34+ cells (R2= 0.975, ratio of class III to class II CD34+ count 1.24 +/- 0.04). The linear relationship between class II and III CD34 epitopes on HPC suggests that the choice of antibody class is not a source of random variation in the quality assurance of CD34+ cell enumeration.
Rudin
C E
CE
Department of Haematology, Royal Devon and Exeter Hospital, University of Exeter.
Garland
J M
JM
Joyner
M V
MV
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34 / Q000276:immunology*; D000939:Epitopes; D005312:Fetal Blood; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D007937:Leukapheresis; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology; D009101:Multiple Myeloma / Q000276:immunology
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D000939:Epitopes; D017493:Leukocyte Common Antigens