Unequal death in T helper cell (Th)1 and Th2 effectors: Th1, but not Th2, effectors undergo rapid Fas/FasL-mediated apoptosis.
T helper cell (Th) 1, but not Th2, effectors undergo rapid Fas/Fas ligand (FasL)-mediated, activation-induced cell death upon restimulation with antigen. Unequal apoptosis is also observed without restimulation, after a longer lag period. Both effectors undergo delayed apoptosis induced by a non-Fas-mediated pathway. When Th1 and Th2 effectors are co-cultured, Th2 effectors survive preferentially, suggesting the responsible factor(s) is intrinsic to each population. Both Th1 and Th2 effectors express Fas and FasL, but only Th2 effectors express high levels of FAP-1, a Fas-associated phosphatase that may act to inhibit Fas signaling. The rapid death of Th1 effectors leading to selective Th2 survival provides a novel mechanism for differential regulation of the two subsets.
Zhang
X
X
University of California, San Diego, La Jolla, California 92093, USA.
Brunner
T
T
Carter
L
L
Dutton
R W
RW
Rogers
P
P
Bradley
L
L
Sato
T
T
Reed
J C
JC
Green
D
D
Swain
S L
SL
D000818:Animals; D000941:Antigens; D017209:Apoptosis; D002352:Carrier Proteins / Q000096:biosynthesis / Q000502:physiology; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis; D053222:Fas Ligand Protein; D007700:Kinetics; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis / Q000502:physiology; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000502:physiology; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D013997:Time Factors; D019014:fas Receptor / Q000502:physiology*
D000941:Antigens; D002352:Carrier Proteins; D016207:Cytokines; C505661:FASLG protein, human; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D011948:Receptors, Antigen, T-Cell; D019014:fas Receptor; C516742:PTPN13 protein, human; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases; C516743:Ptpn13 protein, mouse