Translesional synthesis on DNA templates containing a single abasic site. A mechanistic study of the "A rule".
Site-specifically modified oligodeoxynucleotides containing a single natural abasic site or a chemically synthesized (tetrahydrofuran or deoxyribitol) model abasic site were used as templates for primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I or by calf thymus DNA polymerase alpha. Analysis of the fully extended products of these reactions indicated that both polymerases preferentially incorporate dAMP opposite the natural abasic site and tetrahydrofuran, while DNA templates containing the ring-opened deoxyribitol moiety block translesional synthesis, promoting sequence context-dependent deletions. The frequency of nucleotide insertion opposite the three types of abasic sites follows the order dAMP > dGMP > dCMP > dTMP. The frequency of chain extension was highest when dAMP was positioned opposite a natural abasic site. The frequency of translesional synthesis past abasic sites follows the order tetrahydrofuran > deoxyribose > deoxyribitol. The Klenow fragment promotes blunt end addition of dAMP; this reaction was much less efficient than insertion of dAMP opposite an abasic site. We conclude that the miscoding potential of a natural abasic site in vitro closely resembles that of its tetrahydrofuran analog. Ring-opened abasic sites favor deletions. Studies with polymerase alpha in vitro predict preferential incorporation of dAMP at abasic sites in mammalian cells.
Shibutani
S
S
Department of Pharmacological Sciences, State University of New York, Stony Brook, New York 11794-8651, USA.
Takeshita
M
M
Grollman
A P
AP
D000818:Animals; D002384:Catalysis; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004256:DNA Polymerase I / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism; D004261:DNA Replication; D005663:Furans; D007700:Kinetics; D008956:Models, Chemical; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000378:metabolism; D012255:Ribitol / Q000031:analogs & derivatives; D012997:Solvents; D013329:Structure-Activity Relationship; D013698:Templates, Genetic
D005663:Furans; D009841:Oligonucleotides; D012997:Solvents; C046347:5-deoxy-D-ribitol; C018674:tetrahydrofuran; D012255:Ribitol; D004247:DNA; D004256:DNA Polymerase I; D004257:DNA Polymerase II