The TBP gene from Aspergillus nidulans-structure and expression in Saccharomyces cerevisiae.
The genomic and cDNA copy of the TATA-binding protein (TBP) gene from the filamentous fungus Aspergillus nidulans have been cloned. The gene is interrupted by four introns, one of which is in the long 5' untranslated region of 615 bp. The transcription initiation site was established and the levels of mRNA were analysed under diverse growth conditions and found to vary severalfold. The gene encodes a protein of 268 amino acids composed of an N-terminal domain of 88 amino acids with no significant homology to other TBPs and a C-terminal domain of 180 amino acids with about 95% homology to other fungal TBPs. A cDNA clone under the yeast ADH1 promoter was able to substitute for the yeast TBP gene in vivo; however, the transformants obtained grew poorly at 35 degrees C and on galactose and glycerol at 30 degrees C, though they could grow in the presence of copper ions or aminotriazole at this temperature. This phenotype may be the result of altered function of A. nidulans TBP in certain yeast transcription activation pathways.
Kucharski
Robert
R
Department of Genetics, University of Warsaw Pawińskiego 5a, 02-106 Warsaw, Poland.
Bartnik
Ewa
E
Institute of Biochemistry and Biophysics, Polish Academy of Sciences Pawińskiego 5a, 02-106 Warsaw, Poland.|Department of Genetics, University of Warsaw Pawińskiego 5a, 02-106 Warsaw, Poland.
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016385:TATA Box; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors