title: Offspring of xenogeneically-reconstituted scid/scid mice are capable of a primary xenogeneic immune response to DNP-KLH.
abstract: Human peripheral blood leukocyte (PBL) reconstitution of severe combined immunodeficient (SCID) mice has provided a small animal model system (hu-PBL-SCID) useful for the study of the human immune system and disease pathogenesis. Transfer of xenogeneic PBL from donors other than humans has also been successful; however, the controversy remains regarding the capability of xenogeneically engrafted lymphocytes to mount a primary immune response. Human cells have been identified in offspring from hu-PBL-SCID but were not evaluated for a primary immune response. In the present study, offspring of bovine PBL-reconstituted SCID mice (F1-PBL-SCID-bo) were assessed for specific immune function. Sera from all of the F1-PBL-SCID-bo contained relatively low levels of bovine IgG 5 weeks after birth but bovine Ig became undetectable by 14 or 18 weeks. Eight F1-PBL-SCID-bo (23 or 27 weeks of age) were immunized with a single dose of 100 mu g dinitrophenyl-keyhole limpet hemocyanin (DNP-KLH). Individual cells secreting bovine antibody were enumerated using the ELISA-plaque assay. One week after immunization, bovine cells secreting bovine immunoglobulin (IgG) specific for DNP-KLH were identified in the spleens from three of the F1-PBL-SCID-bo at a frequency of one antibody-secreting cell per 9 x 10(3) to 1 x 10(6) spleen cells. Thus, xenogeneic lymphocytes, passed from the mother to her offspring, retain the capacity for a primary immune response to DNP-KLH.
authors.affiliation: The Wellesley Hospital Research Institute, Toronto, Canada.
authors.forename: J D
authors.initials: JD
authors.lastname: Greenwood
authors.forename: N A
authors.initials: NA
authors.lastname: Bos
authors.forename: B A
authors.initials: BA
authors.lastname: Croy
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000910:Antibodies, Heterophile / Q000096:biosynthesis* / Q000097:blood; D000941:Antigens / Q000008:administration & dosage; D002417:Cattle; D003433:Crosses, Genetic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006241:Haptens / Q000008:administration & dosage; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D017708:Leukocyte Transfusion; D008297:Male; D051379:Mice; D016513:Mice, SCID / Q000276:immunology*; D014183:Transplantation, Heterologous
chemical_list: C042158:2,4-dinitrophenyl keyhole limpet hemocyanin; D000910:Antibodies, Heterophile; D000941:Antigens; D006241:Haptens; D007074:Immunoglobulin G; D006433:Hemocyanins