Expression of NRAMP1 molecule in human peripheral blood leukocytes.
Natural resistance or susceptibility of host to infection with several intracellular pathogens, such as Mycobacterium, Salmonella and Leishmania, is controlled in mice by the expression of a single dominant gene locus designated Lsh/Ity/Bcg. Natural resistance-associated macrophage protein gene 1 (Nramp1) was isolated as a candidate gene. Nramp1 gene encodes a 60 kDa polypeptide with 10-12 potential transmembrane domains and an evolutionary conserved consensus transport motif. The present study shows that the human NRAMP1 gene is expressed in all established hematopoietic cell lines examined, including monocytes/macrophages and B- and T-lymphocytes. In contrast, cell type-specific expressions are observed in human peripheral blood leukocytes. NRAMP1 expression is very low level in granulocytes. B- and T-lymphocytes are equivalent in the level of NRAMP1 expression. Notable expression of NRAMP1 gene can be detected in the monocyte population. These results have important implications for the host defence mechanisms and the pathogenesis of intracellular pathogens which are recognized and ingested by the mononuclear phagocyte system including monocytes/macrophages.
Yoshida
T
T
Ube Research Laboratory, Fujirebio Inc, Yamaguchi, Japan.
Kishi
F
F
D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015153:Blotting, Western; D018801:CD2 Antigens / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology*; D027682:Cation Transport Proteins; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D017452:Receptors, IgG / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014407:Tumor Cells, Cultured; D000099173:Natural Resistance-associated Macrophage Protein 1
D018941:Antigens, CD19; D018801:CD2 Antigens; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D018950:Lipopolysaccharide Receptors; D008565:Membrane Proteins; D017452:Receptors, IgG; D000099173:Natural Resistance-associated Macrophage Protein 1