Structural determination of monosialyl trisaccharides obtained from caprine colostrum.
Acidic oligosaccharides were separated by dialysis, ion-exchange, preparative paper and gel chromatography from caprine colostrum. Four sialyl trisaccharides were characterized by 1H-NMR spectrometry as follows: alpha-N-acetylneuraminyl-(2,6)-beta-D-galactopyranosyl-(1,4)-2-N-acetami do-2 -deoxy-D-glucopyranose (Neu5Ac alpha 2-6Gal beta 1-4GlcNAc), alpha-N-acetylneuraminyl-(2,3)-beta-D-galactopyranosyl-(1,4)-D-glucopyra nose (Neu5Ac alpha 2-3Gal beta-1-4Glc), alpha-N-acetylneuraminyl-(2,6)-beta-D-galactopyranosyl-(1,4)-D-glucopyra nose (Neu5Ac alpha 2-6Gal beta 1-4Glc) and alpha-N-glycolylneuraminyl-(2,6)-beta-D-galactopyranosyl-(1,4)-D -glucopyranose (Neu5Gc alpha 2-6Gal beta 1-4Glc).
Urashima
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T
Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Murata
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S
Nakamura
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T
D000818:Animals; D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D006041:Goats; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid / Q000032:analysis / Q000737:chemistry*; D011247:Pregnancy; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014312:Trisaccharides / Q000737:chemistry*
D014312:Trisaccharides; D019158:N-Acetylneuraminic Acid