title: Effect of hydroxyapatite particle size on myoblasts and fibroblasts.
abstract: After surgery, the bone and soft tissues around integrated biomaterials can be adversely affected by implant-related factors acting over a period of years. However, few studies have directly addressed the effects upon the adjacent soft tissue. The present study was designed to test the biological effects of various sized hydroxyapatite (HA) particles on myoblasts and fibroblasts. Both the myoblasts and fibroblasts were mixed in in vitro culture with 0.1% (1 mg ml(-1)) of various sized HA particles (0.5-3.0, 37-63, 177-250, 420-841 microm) for 1 h, 3 h, 1 day, 3 days and 7 days to test their effects on the cell culture. The results show that adding HA particles into a cell culture can decrease the cell count significantly. The transforming growth factor-beta1 (TGF-beta1) concentrations in the culture medium decreased significantly on addition of HA particles. When calculated as a ratio to the cell number, the TGF-beta1 titre increased most significantly in the groups of medium-sized particles. The prostaglandin E2 (PGE2) concentrations in the medium increased significantly. The changes in TGF-beta1 and PGE2 concentrations with the smallest particles were most significant and persisted longer. The inhibitory effects of the HA particles on the cell culture were mediated by the increased synthesis of PGE2. Caution should be exercised before considering the use of an HA product which could easily break down into a fine powder.
authors.affiliation: Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei, ROC.
authors.forename: J S
authors.initials: JS
authors.lastname: Sun
authors.forename: Y H
authors.initials: YH
authors.lastname: Tsuang
authors.forename: W H
authors.initials: WH
authors.lastname: Chang
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: H C
authors.initials: HC
authors.lastname: Liu
authors.forename: F H
authors.initials: FH
authors.lastname: Lin
mesh_terms: D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D015232:Dinoprostone / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology*; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000187:drug effects
chemical_list: D003470:Culture Media; D016212:Transforming Growth Factor beta; D017886:Durapatite; D015232:Dinoprostone