title: Ability of commercial ligase chain reaction and PCR assays to diagnose Chlamydia trachomatis infections in men by testing first-void urine.
abstract: A total of 287 men (37.6% with symptoms of urethritis) attending a hospital-based sexually transmitted disease clinic had urethral swabs tested by culture and by direct fluorescent-antibody assay. First-void urine (FVU) was tested for Chlamydia trachomatis by commercially available ligase chain reaction (LCR) and PCR assays. By using an expanded reference standard, 35 men (12.2%) were found to be positive. By performing LCR and PCR, the infection prevalence was found to be approximately twice (11.5 and 12.2%, respectively) that determined swab testing. The sensitivity values were 94.3% for LCR and 100% for PCR. One of the two positive specimens missed by LCR contained inhibitors. PCR produced five false-positive results and LCR produced one.
authors.affiliation: McMaster University Regional Virology and Chlamydiology Laboratory, St. Joseph's Hospital, Hamilton, Ontario, Canada.
authors.forename: M A
authors.initials: MA
authors.lastname: Chernesky
authors.forename: S
authors.initials: S
authors.lastname: Chong
authors.forename: D
authors.initials: D
authors.lastname: Jang
authors.forename: K
authors.initials: K
authors.lastname: Luinstra
authors.forename: J
authors.initials: J
authors.lastname: Sellors
authors.forename: J B
authors.initials: JB
authors.lastname: Mahony
mesh_terms: D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D014556:Urine / Q000382:microbiology*