Localization by immunoelectron microscopy of antigens of Chlamydia psittaci suitable for diagnosis or vaccine development.
Two different antigens of serotype 1 Chlamydia psittaci were localized using three immunoelectron microscopy techniques: non-embedding, pre-embedding and post-embedding. The antigens had previously been described as being of potential use in diagnosis (80-90 kDa protein region) and vaccine development (110 kDa protein). The results show a direct relationship between the protective capacity of the antigens and their surface localization on the elementary bodies, which are the infectious form of Chlamydia. The 80-90 kDa protein region is located on the surface of reticulate bodies but not of elementary bodies, where it was located periplasmically, while the 110 kDa protein occurs on the surface of both elementary and reticulate bodies.
Buendia
A J
AJ
Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Murcia, Spain. abuendia@gaia.fcu.um.es
Salinas
J
J
Sánchez
J
J
Gallego
M C
MC
Rodolakis
A
A
Cuello
F
F
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001428:Bacterial Vaccines / Q000276:immunology*; D002691:Chlamydophila psittaci / Q000276:immunology* / Q000302:isolation & purification; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000379:methods*; D018272:Porins; D009956:Psittacosis / Q000175:diagnosis*; D016610:Tissue Embedding
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis