Three different GB virus C/hepatitis G virus genotypes. Phylogenetic analysis and a genotyping assay based on restriction fragment length polymorphism.
The 5'-untranslated region (5'-UTR) sequences of 33 GB virus C/hepatitis G virus (GBV-C/HGV) obtained from different geographic areas were determined through reverse-transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide substitution per site, and construction of phylogenetic trees. The 5'-UTR of GBV-HGV was found to be heterogeneous, with 70.9-99.5% homology. Three distinct phylogenetic branches were observed consistently in all phylogenetic trees. GBV-C is the prototype for one, HGV for another, and there is a new branch which consisted of GBV-C/HGV isolates from Asia. Genotype-specific restriction sites for the restriction enzymes, ScrFI and BsmFI, were identified, and a simple restriction fragment polymorphism analysis was developed for genotyping. These data provide evidence that GBV-C/HGV consists of three different genotypes. Our simple genotyping assay will also provide a tool for epidemiological studies of GBV-C/HGV infection.
Mukaide
M
M
Second Department of Medicine, Nagoya City University Medical School, Mizuho, Nagoya, Japan.
Mizokami
M
M
Orito
E
E
Ohba
K
K
Nakano
T
T
Ueda
R
R
Hikiji
K
K
Iino
S
S
Shapiro
S
S
Lahat
N
N
Park
Y M
YM
Kim
B S
BS
Oyunsuren
T
T
Rezieg
M
M
Al-Ahdal
M N
MN
Lau
J Y
JY
D001483:Base Sequence; D003001:Cloning, Molecular; D018067:Flaviviridae / Q000145:classification*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D012367:RNA, Viral