Inhibition effect of shengma-gegen-tang on measles virus in Vero cells and human peripheral blood mononuclear cells.
Shengma-Gegen-Tang has long been used against measles virus in human peripheral blood mononuclear cells (PBMC) as well as in Vero cells. One hundred micrograms/ml Shengma-Gegen-Tang in PBMC displays significant anti-measles activity, whereas the same concentration in Vero cells does not. After eight days of infection, the release of virus is significantly suppressed by Shengma-Gegen-Tang in the case of PBMC. In addition, Shengma-Gegen-Tang has a selective stimulation to the secretion of cytokine TNF-alpha in PBMC. Time kinetic analysis indicated that the stimulation of secretion was rapid and could be detected only 2 hrs following the treatment of the PBMC. It rose to an optimal level in 8-12 hrs. These findings suggest that the magnification of anti-measles virus activity of this agent is lymphocyte dependent and may well be mediated by TNF-alpha.
Huang
S P
SP
Institute of Chinese Medicine and Pharmacy, Department of Health, Nan-Kang, Taipei, Taiwan.
Shieh
G J
GJ
Lee
L
L
Teng
H J
HJ
Kao
S T
ST
Lin
J G
JG
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000821:virology*; D008457:Measles / Q000188:drug therapy; D008459:Measles virus / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014709:Vero Cells / Q000166:cytology / Q000187:drug effects / Q000821:virology*; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma