Ontogeny of glutamic acid decarboxylase gene expression in the mouse pancreas.
Using two RT-PCR quantitative assays, we measured the pancreatic expression of the two isoforms of glutamic acid decarboxylase in the foetus, newborn and 14-, 21- and 35-day-old male and female NOD and C57BL/6 mice. In the C57BL/6 mouse, GAD 67 pancreatic expression is stable; in NOD mice, GAD 67 expression is similar to that found in control mice, except at 5 weeks of age, when pancreatic GAD 67 expression is about 2.5 times higher than in C57BL/6 mice. The pancreatic expression of GAD 65 is under the detection limit of the assay until 5 weeks of age. The overexpression of GAD 67 characterized in pancreas from 5-week-old NOD mice could be the result of beta cell hyperactivity, previously reported in this mouse strain.
Pleau
J M
JM
CNRS URA 1461, Université Paris V, Hôpital Necker, France. dardenne@infobiogen.fr
Throsby
M
M
Esling
A
A
Dardenne
M
M
D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005968:Glutamate Decarboxylase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D010179:Pancreas / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development
D007527:Isoenzymes; D005968:Glutamate Decarboxylase