title: Diurnal variations of interleukin-1 beta mRNA and beta-actin mRNA in rat brain.
abstract: Interleukin-1 beta (IL-1 beta) is posited to play an important physiological role in brain functions in addition to its better defined role in pathology. The experiments described herein were performed to determine if IL-1 beta mRNA and beta-actin display diurnal rhythms in various areas of brain. Rats were sacrificed at 4 h intervals across a 12:12 h light/dark cycle. Hypothalamic, hippocampal and cortical IL-1 beta mRNA peaked just after lights were turned on, declined slightly during the remaining light period and stayed low in the dark. There were no significant changes in IL-1 beta mRNA in brain stem or cerebellum samples. beta-actin mRNA levels were relatively constant across the day in the hypothalamus, brain stem and cerebellum. However, beta-actin mRNA levels were lower during the day than during the night in the hippocampus and cortex.
authors.affiliation: Department of Physiology and Biophysics, University of Tennessee, Memphis 38163, USA.
authors.forename: P
authors.initials: P
authors.lastname: Taishi
authors.forename: S
authors.initials: S
authors.lastname: Bredow
authors.forename: N
authors.initials: N
authors.lastname: Guha-Thakurta
authors.forename: F
authors.initials: F
authors.lastname: Obál
authors.forename: J M
authors.initials: JM
authors.lastname: Krueger
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002940:Circadian Rhythm; D007375:Interleukin-1 / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D007375:Interleukin-1; D012333:RNA, Messenger