Binding of the influenza virus NS1 protein to model genome RNAs.
We have previously shown that the influenza virus NS1 protein exhibits stable binding to a model mini vRNA which is stronger than its binding to dsRNA. In this study, we confirmed that the binding depended on a higher-order structure of the model RNA, probably the panhandle structure formed by pairing between the 5'- and 3'-terminal common sequences. The formation of the structure was enhanced by the NS1 protein itself. We propose that an A bulge in a stretch of double helix results in a binding site with greater affinity for the NS1 protein.
Hatada
E
E
Department of Biochemistry, Kanazawa University School of Medicine, Ishikawa, Japan.
Saito
S
S
Okishio
N
N
Fukuda
R
R
D016678:Genome; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA / Q000378:metabolism*; D013696:Temperature; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
C085820:INS1 protein, influenza virus; D017361:Viral Nonstructural Proteins; D012313:RNA