Immunological detection of lipopolysaccharide antigens of thermophilic campylobacters captured on polymyxin-coated polyester cloth.
Cholate-extracted lipopolysaccharide (LPS) antigens from thermophilic campylobacters were captured on polymyxin-coated polyester cloth. The captured antigens were detected by sequential reactions with rabbit anti-Campylobacter antibody, anti-rabbit IgG peroxidase conjugate and chromogenic peroxidase substrate. A polyclonal rabbit antibody elicited against a single Campylobacter-jejuni strain detected the reference strains of the twenty most frequently isolated thermophilic campylobacters in the Lior serotyping scheme. Moreover, LPS antigens of six C. Jejuni Penner serotypes fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and examined by immunoblotting were recognized by four antisera prepared against homologous and heterologous Penner serotypes. The results suggest the potential application of polymyxin-cloth enzyme immunoassay for rapid detection of thermophilic campylobacters where monoclonal antibodies can be raised to possible common LPS epitopes.
Gomi
K
K
Department of Biology, Carleton University, Ottawa, Canada.
Brooks
B
B
Blais
B
B
Garcia
M
M
Yamazaki
H
H
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D002167:Campylobacter / Q000276:immunology* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D000939:Epitopes / Q000032:analysis; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D011091:Polyesters; D011113:Polymyxins; D011817:Rabbits; D012703:Serotyping
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D011091:Polyesters; D011113:Polymyxins