Time course of the effects of a high-fat diet and voluntary exercise on muscle enzyme activity in Long-Evans rats.
This study examined the time course of the effects of a high-fat diet and voluntary running exercise on rat skeletal muscle carnitine acyltransferase (CAT), beta-hydroxy-acyl-CoA dehydrogenase (HAD), and citrate synthase (CS) activities. Sixty male Long-Evans rats were randomly allocated to receive either a standard (12% fat by energy) laboratory chow diet (CHOW) or a high-fat (76% by energy) diet (HFD) and placed in running wheels for up to 6 weeks. Energy intakes and weekly voluntary running distances were similar in the CHOW and HFD rats. In both groups, weekly training distance more than doubled from week 4 to week 6. However, increased training had little influence on soleus (s) CAT(s), HAD(s), and CS(s) activities. CAT(s) and HAD(s) activities were higher in the HFD rats than in the CHOW rats from 2 weeks onward (p < 0.005), and CS(s) activities were not different between groups and remained constant over time. In contrast, increased training distance after 4 weeks in the CHOW rats resulted in an increase in deep vastus (v) CAT(v) activities to values similar to those in HFD rats prior to increases in training volume (p < 0.005) but had no effect on their HAD(v) and CS(v) activities. Increases in HAD(v) and CS(v) activities with increased training volume were only seen in the HFD rats (p < 0.005). HAD(v) activities and HAD/CS(v) activity ratios correlated with training distance in the HFD rats only (p < 0.001 and p < 0.01, respectively). These results suggest that a high-fat diet improves the beta-oxidation capacity of rat predominantly slow-twitch soleus muscle and enhances the effects of modest levels of training on the mitochondrial density and beta-oxidation capacity of rat deep vastus mixed fast- and slow-twitch muscles.
Cheng
B
B
Department of Physiology, University of Cape Town Medical School, Sport Science Institute of South Africa.
Karamizrak
O
O
Noakes
T D
TD
Dennis
S C
SC
Lambert
E V
EV
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism*; D000818:Animals; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism*; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D008297:Male; D008931:Mitochondria, Muscle / Q000201:enzymology; D018656:Muscle Fibers, Fast-Twitch / Q000201:enzymology; D018657:Muscle Fibers, Slow-Twitch / Q000201:enzymology; D018482:Muscle, Skeletal / Q000201:enzymology*; D010084:Oxidation-Reduction; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats
D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D015094:3-Hydroxyacyl CoA Dehydrogenases; D002332:Carnitine O-Acetyltransferase; D002950:Citrate (si)-Synthase