Expression of MHC class II CD4+ and ED1 molecules in association with selective hippocampal neuronal degeneration after long-term adrenalectomy.
The neuroendocrine and the immune systems are interconnected. Monoclonal antibodies against major histocompatibility complex (MHC) class I, class II, CD4, CD8, pan T cells, and macrophages were used for immunostaining brains from adrenalectomized (ADX) and shamoperated rats to investigate the potential involvement of the immune/inflammatory mechanisms in the neurodegeneration of hippocampus after ADX. Our results demonstrate upregulation of MHC class II, CD4 antigens and activated microglial marker-ED1 expression selectively in the hippocampus after ADX. The absence of CD5 reactivity precludes that these activated cells were T lymphocytes. The activated microglial cells may either be instrumental in the hippocampal neuronal loss or activated secondarily to the neuronal degeneration after long-term adrenalectomy.
Islam
A
A
Department of Clinical Neuroscience and Family Medicine, Huddinge Hospital, Sweden.
Mustafa
M
M
Mustafa
A
A
Olsson
T
T
Winblad
B
B
Adem
A
A
D000315:Adrenalectomy; D000818:Animals; D015415:Biomarkers; D015704:CD4 Antigens / Q000096:biosynthesis*; D006624:Hippocampus / Q000276:immunology / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D017628:Microglia / Q000276:immunology / Q000502:physiology*; D009410:Nerve Degeneration; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D015704:CD4 Antigens; D000949:Histocompatibility Antigens Class II