title: Derivatization and high-performance liquid chromatographic analysis of pentaazapentacosane pentahydrochloride.
abstract: A rapid high-performance liquid chromatographic method for determination of the dansyl derivative of pentaazapen-tacosane (PAPC) pentahydrochloride has been developed. The chromatographic system uses a reversed-phase C8 column, a mobile phase of acetic acid buffer and acetonitrile and UV detection. The dansylation conditions were optimized with a pH of 11.0 and a 20-fold dansyl chloride excess. The yield of dansyl PAPC increased 10-fold as the reaction pH was changed from 9.5 to 10.5. Under derivatization conditions of pH 8.5-11.0 and 1-30-fold excess dansyl chloride only perdansyl PAPC was found.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of Arizona, Tucson 85721, USA.
authors.forename: S
authors.initials: S
authors.lastname: Heimbecher
authors.forename: Y C
authors.initials: YC
authors.lastname: Lee
authors.forename: S E
authors.initials: SE
authors.lastname: Tabibi
authors.forename: S H
authors.initials: SH
authors.lastname: Yalkowsky
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D013096:Spermine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000970:Antineoplastic Agents; D003619:Dansyl Compounds; C082768:BE 4-4-4-4; D013096:Spermine