Insights into the mechanism of action of benzoyl peroxide as a tumor promoter.
A comparison of the mechanism of action of benzoyl peroxide, a tumor promoter was studied in three different cell lines i.e. NIH 3T3, HDCS and A431. Benzoyl peroxide was found to mediate its effect by inducing poly ADP-ribosylation in all the three cell types studied but to different extents, with histone H1 serving as a common acceptor for poly ADP-ribose. It also stimulated the activities of the antioxidant enzymes CuZn superoxide dismutase and catalase in NIH 3T3 and HDCS cells, but not in A431. Alterations in the expression of c-jun and c-fos were observed in NIH 3T3 and A431 cells. Benzoyl Peroxide appeared to mediate its effect via genetic and epigenetic mechanisms.
Rudra
N
N
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
Singh
N
N
D016475:3T3 Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D001585:Benzoyl Peroxide / Q000633:toxicity*; D015152:Blotting, Northern; D002273:Carcinogens / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D016762:Genes, fos / Q000187:drug effects / Q000235:genetics; D016758:Genes, jun / Q000187:drug effects / Q000235:genetics; D006657:Histones; D007641:Keratolytic Agents / Q000633:toxicity*; D051379:Mice; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000201:enzymology
D002273:Carcinogens; D006657:Histones; D007641:Keratolytic Agents; D011064:Poly Adenosine Diphosphate Ribose; D002374:Catalase; D013482:Superoxide Dismutase; D011065:Poly(ADP-ribose) Polymerases; D011493:Protein Kinase C; D001585:Benzoyl Peroxide