Description of a new HLA-E (E*01031) allele and its frequency in the Spanish population.
An HLA-E polymorphism study by oligotyping and DNA sequencing was carried out in the Spanish population. As a result, a new HLA-E allele (E*01031) initially assigned by polymerase chain reaction oligotyping as E*0104 was found. This allele presents a synonymous change at codon 77 (AAT-->AAC; Asn) when compared with the E*01032 allele. This position is located in the alpha-helix (alpha 1-domain) and is involved in the peptide binding region of the hypothetical HLA-E molecule. Among 60 Spanish individuals, HLA-E*0101 presents the highest phenotype frequency, followed in decreasing order by E*01032, E*01031 (new allele), and E*0102. Also, new partial intron 1 and complete intron 2 sequences from E*0101, E*01031, and E*01032 are described; the sequences are identical among the three forms. However, the intron 2 sequence of the E*0102 allele bears a two-base deletion not found in apes.
Gomez-Casado
E
E
Department of Immunology, Hospital 12 de Octubre, Universidad Complutense, Madrid, Spain.
Martinez-Laso
J
J
Vargas-Alarcón
G
G
Varela
P
P
Diaz-Campos
N
N
Alvarez
M
M
Alegre
R
R
Arnaiz-Villena
A
A
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D005787:Gene Frequency / Q000276:immunology*; D005805:Genes, MHC Class I / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000276:immunology; D013030:Spain; D000097625:HLA-E Antigens
D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens