The major component of Na-CBZ-L-lysine thiobenzyl ester (BLT)-specific proteases in cytoplasmic granules of murine intraepithelial lymphocytes is granzyme A.
Na-CBZ-L-lysine thiobenzyl ester (BLT)-specific proteases in cytoplasmic granules of intraepithelial lymphocytes in the murine intestine (iIEL) were characterized. BLT-specific proteases were isolated with the Sephacryl S-200 column chromatography, and the sample isolated contained a protein with a molecular weight of 58 kDa. The 58 kDa protein consisted of the homodimer of the 30 kDa subunits. The 58 kDa protease was detected by [3H] diisopropylfluorophosphate (DFP)-labeling, and also detectable by the immunoblotting using an antibody against the partial synthetic peptide of granzyme A. The cytoplasmic granules of iIEL were stained positively by an immunofluorescence with anti-granzyme A antibody. Therefore, it was suggested that the major BLT-specific proteases present in cytoplasmic granules of iIEL might be granzyme A.
Kato
Y
Y
Department of Microbiology and Immunology, Aichi Medical University, Nagakute, Japan.
Yokochi
T
T
Sasaki
K
K
Kawamoto
Y
Y
Tsuji
T
T
Miyama
A
A
D000818:Animals; D003594:Cytoplasmic Granules / Q000201:enzymology* / Q000276:immunology / Q000648:ultrastructure; D004848:Epithelium / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure; D005260:Female; D053804:Granzymes; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000276:immunology / Q000648:ultrastructure; D007421:Intestine, Small / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D013379:Substrate Specificity / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology* / Q000648:ultrastructure
D053804:Granzymes; D012697:Serine Endopeptidases