Local delivery of interleukin 4 by retrovirus-transduced T lymphocytes ameliorates experimental autoimmune encephalomyelitis.
Experimental autoimmune encephalomyelitis (EAE) is an inflammatory autoimmune disease of the central nervous system which serves as a model for the human disease multiple sclerosis. We demonstrate here that encephalitogenic T cells, transduced with a retroviral gene, construct to express interleukin 4, and can delay the onset and reduce the severity of EAE when adoptively transferred to myelin basic protein-immunized mice. Thus, T lymphocytes transduced with retroviral vectors can deliver "regulatory cytokines" in a site-specific manner and may represent a viable therapeutic strategy for the treatment of autoimmune disease.
Shaw
M K
MK
Department of Medicine, Stanford University School of Medicine, California 94305-5111, USA.
Lorens
J B
JB
Dhawan
A
A
DalCanto
R
R
Tse
H Y
HY
Tran
A B
AB
Bonpane
C
C
Eswaran
S L
SL
Brocke
S
S
Sarvetnick
N
N
Steinman
L
L
Nolan
G P
GP
Fathman
C G
CG
D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000628:therapy*; D015316:Genetic Therapy; D005822:Genetic Vectors; D007116:Immunization, Passive; D007167:Immunotherapy; D016753:Interleukin-10 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000008:administration & dosage* / Q000096:biosynthesis; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D013601:T-Lymphocytes; D014161:Transduction, Genetic
D004676:Myelin Basic Protein; D011948:Receptors, Antigen, T-Cell; D016753:Interleukin-10; D015847:Interleukin-4