Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens.
We describe a method for the production of baculovirus-based cDNA libraries. By staining with monoclonal antibodies, single positive cells can be sorted and the virus encoding for the surface epitope can be isolated by limiting dilution. We have used this method to isolate cDNAs encoding several cell-surface antigens.
Granziero
L
L
Basle Institute for Immunology, Switzerland.
Nelboeck
P
P
Bedoucha
M
M
Lanzavecchia
A
A
Reid
H H
HH
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D016367:Baculoviridae / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics*; D015723:Gene Library; D005822:Genetic Vectors / Q000276:immunology*; D006801:Humans; D010920:Placenta / Q000378:metabolism / Q000821:virology; D011995:Recombination, Genetic; D018411:Spodoptera / Q000235:genetics / Q000821:virology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D018076:DNA, Complementary; D004279:DNA, Viral