[Molecular genetic nature of mutations at the white locus, induced by chemical substances in Drosophila melanogaster].
Molecular genetic mechanisms of the white mutations induced by mitomycin C, N-ethyl-N-nitrosourea, and ethidium bromide were studied. Genomic DNA of the original strain and mutants obtained was tested by Southern blot hybridization. The presence of mobile genetic elements was shown to be characteristic of the white locus of the original strain. Mutations of the white gene obtained mainly resulted from excision of DNA sequences involving mobile genetic elements and insertion of unidentified 5-6-kbp DNA fragments.
Shcherbata
G R
GR
Maksymiv
D V
DV
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D004996:Ethidium / Q000633:toxicity; D005038:Ethylnitrosourea / Q000633:toxicity; D016678:Genome; D016685:Mitomycin / Q000633:toxicity*; D016254:Mutagenesis, Insertional; D009153:Mutagens / Q000633:toxicity*
D004251:DNA Transposable Elements; D009153:Mutagens; D016685:Mitomycin; D004996:Ethidium; D005038:Ethylnitrosourea
Molekuliarno-geneticheskaia priroda mutatsiĭ po lokusu white, indutsirovannykh khimicheskimi veshchestvami u Drosophila melanogaster.