[Detection of Neisseria meningitidis group B antigens by MB-Dot-Elisa test in patients with meningitis].
Infection with Neisseria meningitidis group B has been difficult to detect, partly because this bacterial group's polysaccharide is a weak immunogen. This article describes work carried out to test a new procedure (MB-Dot-ELISA) employing a high-titered horse antiserum for detection of N. meningitidis group B antigens. The study assayed cerebrospinal fluid samples from 585 subjects, 574 with suspected meningitis cases and 11 with neurologic disorders. The results of the assay indicated a sensitivity of 0.991 and a specificity of 0.826. These results were superior to those obtained with latex agglutination and in substantial agreement with the results of counterimmunoelectrophoresis and bacteriologic methods. Overall, the MB-Dot-ELISA was found to be sensitive, inexpensive, and suitable for public health laboratory investigations.
Alkmin
M G
MG
Instituto Adolfo Lutz, Secciíon de Inmunología, São Paulo, Brasil.
Landgraf
I M
IM
Shimizu
S H
SH
D000818:Animals; D000942:Antigens, Bacterial / Q000134:cerebrospinal fluid*; D001431:Bacteriological Techniques; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D006736:Horses; D006801:Humans; D007106:Immune Sera; D007841:Latex Fixation Tests; D008585:Meningitis, Meningococcal / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000276:immunology; D008853:Microscopy; D009345:Neisseria meningitidis / Q000145:classification / Q000276:immunology*; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D007106:Immune Sera
Detección de antígenos de Neisseria meningitidis del grupo B con la prueba MB-Dot-ELISA en pacientes con meningitis.