Pancreas-specific protein (PASP), serum amyloid A (SAA), and neopterin (NEOP) in the diagnosis of rejection after simultaneous pancreas and kidney transplantation.
A reliable, noninvasive indicator of pancreatic allograft rejection is urgently needed. In this study, serum (S), plasma (P), and urine (U) levels of pancreas-specific protein (P-PASP, U-PASP), neopterin (S-NEOP, U-NEOP), amylase (U-AMYL), and amyloid A (SAA) were measured daily in ten type I diabetic patients following simultaneous pancreas and kidney transplantation (SPK). Rejection episodes occurred in three isolated pancreas, nine isolated kidney, and five simultaneous pancreas and kidney transplants. In the case of the eight pancreas rejections, SAA was the rejection marker with the highest diagnostic accuracy (94%). Using P-PASP and U-PASP, an accuracy of 81% and 79%, respectively, was achieved. During viral infections, U-NEOP levels increased to a maximum level of 1904 mumol/mol creatinine, whereas during bacterial infections, SAA levels increased to a maximum value of 43 mg/dl. SAA, measured for the first time in SPK, appears to be a valuable rejection parameter. In combination with U-NEOP and U-AMYL, a differential diagnosis between rejection, bacterial infection, and viral infection was possible. Neither U-PASP nor P-PASP monitoring led to a significant improvement in the results.
Müller
T F
TF
Department of Nephrology, Philipps-University of Marburg, Germany.
Trösch
F
F
Ebel
H
H
Grüssner
R W
RW
Feiber
H
H
Göke
B
B
Greger
B
B
Lange
H
H
D000208:Acute Disease; D000328:Adult; D001053:Apolipoproteins / Q000032:analysis*; D001424:Bacterial Infections / Q000175:diagnosis; D001708:Biopterins / Q000031:analogs & derivatives* / Q000097:blood; D043424:Carboxypeptidase B; D002268:Carboxypeptidases; D003922:Diabetes Mellitus, Type 1 / Q000601:surgery; D005260:Female; D006084:Graft Rejection / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D006801:Humans; D016030:Kidney Transplantation / Q000379:methods*; D008297:Male; D019798:Neopterin; D016035:Pancreas Transplantation / Q000379:methods*; D010865:Pilot Projects; D011506:Proteins / Q000032:analysis*; D000685:Serum Amyloid A Protein / Q000032:analysis*; D014777:Virus Diseases / Q000175:diagnosis
D001053:Apolipoproteins; C058173:CPB1 protein, human; D011506:Proteins; D000685:Serum Amyloid A Protein; D001708:Biopterins; D019798:Neopterin; D002268:Carboxypeptidases; D043424:Carboxypeptidase B