CTLL assay: comparison of two methods for IL-2 determination.
Two different modifications of CTLL techniques for IL-2 detection, the routine 3II-thymidine incorporation assay and the colorimetric MTT method, were compared. The reproducible results of the studies of supernatants from in vitro propagated transfected cells were obtained. The employed methods appeared to be comparable and are used simultaneously in our co-operating laboratories for estimation of the levels of IL-2 secreted by the transfectants that are applied as the source of the cytokine in therapeutic experiments.
Indrová
M
M
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Pajtasz-Piasecka
E
E
Radzikowski
C
C
Bubeník
J
J
D000818:Animals; D002478:Cells, Cultured; D003124:Colorimetry / Q000379:methods; D003602:Cytotoxicity, Immunologic; D018076:DNA, Complementary; D007376:Interleukin-2 / Q000032:analysis* / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D010954:Plasmacytoma; D011865:Radioisotope Dilution Technique; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D015203:Reproducibility of Results; D013936:Thymidine / Q000378:metabolism; D014162:Transfection; D014316:Tritium; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D007376:Interleukin-2; D011994:Recombinant Proteins; D014316:Tritium; D013936:Thymidine