Comparative physiology of salt tolerance in Candida tropicalis and Saccharomyces cerevisiae.
The salt tolerance of the respiratory yeast Candida tropicalis and the fermentative yeast Saccharomyces cerevisiae have been compared in glucose media. C. tropicalis showed a better adaptation to Na+ and Li+ and maintained higher intracellular K+:Na+ and K+:Li+ ratios than S. cerevisiae. However, C. tropicalis showed a poorer adaptation to osmotic stress (produced by KCl and sorbitol) and exhibited reduced glycerol production as compared to S. cerevisiae. In media with the non-repressing sugar galactose as carbon source, S. cerevisiae exhibited reduced glycerol production and increased sensitivity to osmotic stress. Under these conditions, S. cerevisiae, but not C. tropicalis, utilized trehalose as a more important osmolyte than glycerol. These results suggest that the relative tolerance of yeast to the osmotic and cation toxicities of NaCl, and the underlying relative capabilities for osmolyte synthesis and cation transport, are modulated by the general catabolite control exerted by glucose.
García
María J
MJ
Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
Ríos
Gabino
G
Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
Ali
Rashid
R
Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
Bellés
Jose M
JM
Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
Serrano
Ramón
R
Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
D000222:Adaptation, Physiological; D002175:Candida / Q000502:physiology*; D005990:Glycerol / Q000378:metabolism; D006706:Homeostasis; D018021:Lithium Chloride / Q000378:metabolism / Q000494:pharmacology; D009997:Osmotic Pressure; D011189:Potassium Chloride / Q000378:metabolism / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D012492:Salts / Q000378:metabolism* / Q000494:pharmacology; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D013012:Sorbitol / Q000378:metabolism / Q000494:pharmacology; D013045:Species Specificity; D014199:Trehalose / Q000378:metabolism
D012492:Salts; D012965:Sodium Chloride; D013012:Sorbitol; D011189:Potassium Chloride; D014199:Trehalose; D018021:Lithium Chloride; D005990:Glycerol