title: beta-Glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitidis.
abstract: Wild-type cells of the pathogenic black yeast Exophiala (Wangiella) dermatitidis grown in a low-pH ascorbate medium became less melanized and less resistant to Zymolyase. This was accompanied by increased staining with fluorescently labelled concanavalin A. The sugar composition of wild-type and mutant cell walls was, except for the presence of galactose, similar to that of Saccharomyces cerevisiae. Digestion of mutant cell walls with laminarinase released galactomannoproteins. In addition, the released cell wall proteins contained glucose and reacted with affinity-purified 1,6-beta-glucan antiserum, indicating that they are linked to 1,6-beta-glucan. It is proposed that 1,6-beta-glucosylated cell wall proteins generally occur among ascomycetes.
authors.affiliation: Institute of Molecular Cell Biology, University of Amsterdam, Biocentrum Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
authors.forename: Roy C
authors.initials: RC
authors.lastname: Montijn
authors.affiliation: Institute of Molecular Cell Biology, University of Amsterdam, Biocentrum Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
authors.forename: Patricia
authors.initials: P
authors.lastname: Van Wolven
authors.affiliation: Centraalbureau voor Schimmelcultures, PO Box 273, Baarn, The Netherlands.
authors.forename: Sybren
authors.initials: S
authors.lastname: De Hoog
authors.affiliation: Institute of Molecular Cell Biology, University of Amsterdam, Biocentrum Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
authors.forename: Frans M
authors.initials: FM
authors.lastname: Klis
mesh_terms: D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000737:chemistry*; D003470:Culture Media; D005093:Exophiala / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D005690:Galactose / Q000032:analysis; D005936:Glucans / Q000302:isolation & purification; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D008358:Mannose / Q000032:analysis; D008543:Melanins / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D005936:Glucans; D006023:Glycoproteins; D008543:Melanins; D008562:Membrane Glycoproteins; C017336:galactoproteins; C025363:mannoproteins; D008358:Mannose; D005690:Galactose