Characterization of Chlamydia trachomatis l2-induced tyrosine-phosphorylated HeLa cell proteins by two-dimensional gel electrophoresis.
Chlamydia trachomatis is an obligate intracellular bacteria, inducing its own uptake in nonprofessional phagocytes either by phagocytosis or pinocytosis. We have previously shown that C. trachomatis L2 induces tyrosine phosphorylation of eukaryotic proteins upon their entry by phagocytosis. In this paper we characterize the tyrosine-phosphorylated proteins by two-dimensional gel electrophoresis. In immunoblotting with anti-phosphotyrosine antibodies of C. trachomatis L2-infected HeLa cells, but not with uninfected cells, two rows of spots were observed with a molecular mass of 69 and 71 kDa and pI from 5.0 to 5.2. In addition, a single spot of 100 kDa and pI 6.2 was observed.
Birkelund
S
S
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark.
Bini
L
L
Pallini
V
V
Sanchez-Campillo
M
M
Liberatori
S
S
Clausen
J D
JD
Ostergaard
S
S
Holm
A
A
Christiansen
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002692:Chlamydia trachomatis / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis*; D014443:Tyrosine / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D019000:Phosphotyrosine; D014443:Tyrosine