[Interleukin-8 is an important inflammatory mediator in hemorrhagic rectocolitis and Crohn disease].
OBJECTIVE
The aim of the study was to assess in vitro the production of interleukin 8 in Crohn's disease and ulcerative colitis.


METHODS
Interleukin 8 concentrations (measured by ELISA) were evaluated in the culture supernatants of: a) peripheral blood mononuclear cells from 13 patients with Crohn's disease, 7 patients with ulcerative colitis and 7 controls, b) organ cultures of inflamed (22 Crohn's disease, 15 ulcerative colitis) and uninflamed (21 Crohn's disease, 9 ulcerative colitis, 15 controls) intestinal mucosal biopsies.


RESULTS
In patients with Crohn's disease and ulcerative colitis, the production of interleukin 8 by peripheral blood mononuclear cells and inflamed mucosa was higher than in controls (P < 0.01) without statistical difference between Crohn's disease and ulcerative colitis. Interleukin 8 production by normal mucosa was not different between the 3 groups. Interleukin 8 concentrations in the supernatants of organ culture were positively correlated to an endoscopical and a histological inflammatory index as well as to tumor necrosis factor, interleukin 1 and 6 concentrations.


CONCLUSIONS
These results support the notion that interleukin 8 plays an important role in the pathogenesis of inflammatory bowel disease.
Reimund
J M
JM
Départment d'Immunologie, Immunopharmacologie et Pathologie, CNRS, Centre de Recherches Pharmaceutiques, Illkirch-Graffenstaden, Strasbourg.
Duclos
B
B
Dumont
S
S
Muller
C
C
Baumann
R
R
Poindron
P
P
D000328:Adult; D001706:Biopsy; D002478:Cells, Cultured; D003093:Colitis, Ulcerative / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D003424:Crohn Disease / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis*; D016209:Interleukin-8 / Q000032:analysis* / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D018836:Inflammation Mediators; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha
L'interleukine-8 est un médiateur inflammatoire important, au cours de la rectocolite hémorragique et de la maladie de Crohn.