title: Flow cytometric analysis of band 3 protein of human erythrocytes.
abstract: Flow cytometry was used to quantify the transmembrane anion exchanger (band 3 protein) of human erythrocytes by covalently bound eosin-5-maleimide. In vitro and in vivo vesiculated red blood cells were investigated. The fluorescence and light scatter signals of cells after heat induced vesiculation, in vivo ageing, and in patients with hereditary spherocytosis were decreased. These results reflect a deficiency of band 3 protein which is presumably caused by membrane surface area loss. It was possible to distinguish control erythrocytes, erythrocytes from patients with hereditary spherocytosis, and from other forms of haemolytic anaemias on the basis of their light scatter and fluorescence signals characteristics.
authors.affiliation: Institute of Anatomy I, Friedrich Schiller University Jena, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Stoya
authors.forename: E
authors.initials: E
authors.lastname: Baumann
authors.forename: U
authors.initials: U
authors.lastname: Junker
authors.forename: J
authors.initials: J
authors.lastname: Hermann
authors.forename: W
authors.initials: W
authors.lastname: Linss
mesh_terms: D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry* / Q000172:deficiency / Q000378:metabolism; D002469:Cell Separation; D004801:Eosine Yellowish-(YS) / Q000031:analogs & derivatives; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D008027:Light; D012542:Scattering, Radiation; D013103:Spherocytosis, Hereditary / Q000097:blood / Q000175:diagnosis
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; C033531:eosin maleimide; D004801:Eosine Yellowish-(YS)