Molecular cloning and comparative analysis of the rhesus macaque costimulatory molecules CD80 (B7-1) and CD86 (B7-2).
To facilitate analysis of the role of costimulatory molecules in a nonhuman primate model, we cloned and sequenced the CD80 (B7-1) and CD86 (B7-2) costimulatory molecules from rhesus macaques. Rhesus CD80 and CD86 were highly homologous to their human counterparts, with overall amino acid homologies of greater than 90%, and were specifically recognized by murine antihuman CD80 or CD86 monoclonal antibodies. Stable cell lines expressing rhesus CD80 or CD86 induced proliferation of suboptimally activated CD4+ T cells and transcription of cytokine mRNA. Both CD80 and CD86 were able to provide costimulation for interferon-gamma and IL-2 synthesis by rhesus CD4+ T cells, but CD80 costimulation also resulted in synthesis of IL-4 and IL-10. The high degree of homology between the rhesus and the human CD80 and CD86 molecules should facilitate analysis of therapeutic interventions directed at this costimulatory pathway in nonhuman primates.
Zhang
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Division of Immunology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA.
Johnson
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D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D018122:B7-1 Antigen / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D051940:B7-2 Antigen; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D008253:Macaca mulatta; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D016207:Cytokines; D008562:Membrane Glycoproteins