Emergence of the trimethoprim resistance gene dfrD in Listeria monocytogenes BM4293.
The sequence of the trimethoprim resistance gene of the 3.7-kb plasmid (pIP823) that confers high-level resistance (MIC, 1,024 microg/ml) to Listeria monocytogenes BM4293 was determined. The gene was identical to dfrD recently detected in Staphylococcus haemolyticus MUR313. The corresponding protein, S2DHFR, represents the second class of high-level trimethoprim-resistant dihydrofolate reductase identified in gram-positive bacteria. We propose that trimethoprim resistance in L. monocytogenes BM4293 could originate in staphylococci.
Charpentier
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E
Unité des Agents Antibactériens, Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
Courvalin
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P
D000595:Amino Acid Sequence; D008089:Listeria monocytogenes / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D014296:Trimethoprim Resistance / Q000235:genetics*