title: A micro-dissection approach for isolation of NotI linking clones from regions frequently deleted in RCC and SCLC.
abstract: We demonstrate that micro-dissection can be used for isolating NotI linking clones from the human 3p21-pter region. This approach is an improvement to positional cloning techniques, since NotI linking clones are directly linked with genes.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. Andrei.Alimov@mtc.ki.se
authors.forename: A A
authors.initials: AA
authors.lastname: Alimov
authors.forename: M A
authors.initials: MA
authors.lastname: Rodova
authors.forename: R Z
authors.initials: RZ
authors.lastname: Gizatullin
authors.forename: M Y
authors.initials: MY
authors.lastname: Kost-Alimova
authors.forename: L I
authors.initials: LI
authors.lastname: Fedorova
authors.forename: V E
authors.initials: VE
authors.lastname: Barsky
authors.forename: V
authors.initials: V
authors.lastname: Kashuba
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: E R
authors.initials: ER
authors.lastname: Zabarovsky
authors.forename: A V
authors.initials: AV
authors.lastname: Zelenin
mesh_terms: D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular / Q000379:methods*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D008846:Micromanipulation / Q000379:methods*; D008969:Molecular Sequence Data
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases