Use of enzyme-linked immunosorbent assay techniques with cross-reacting human sera in diagnosis of murine typhus and spotted fever.
Enzyme-linked immunosorbent assay (ELISA) techniques for the determination of immunoglobulin G to rickettsial lipopolysaccharides were developed. These techniques provide a simple and convenient way to serodiagnose Mediterranean spotted fever and murine typhus with a single serum dilution. The results of the ELISAs correlated with the indirect immunofluorescence assay titers of cross-reacting sera.
Keysary
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Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, Israel.
Strenger
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D000818:Animals; D001907:Boutonneuse Fever / Q000175:diagnosis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D012281:Rickettsia / Q000276:immunology / Q000302:isolation & purification*; D014437:Typhus, Endemic Flea-Borne / Q000175:diagnosis*