Rapid diagnosis of enterovirus infection by a new one-step reverse transcription-PCR assay.
The AMPLICOR Enterovirus Test was evaluated with 103 cerebrospinal fluid (CSF) specimens. Twenty-seven CSF specimens were culture positive. With the AMPLICOR test, enterovirus RNA was detected in 34 specimens. Compared with culture, the AMPLICOR test gave a sensitivity of 96.3% and a specificity of 100%. The sensitivity of culture was 79.4% in comparison with the AMPLICOR test.
Kessler
H H
HH
Institute of Hygiene, KF-University Graz, Austria. harald.kessler@kfunigraz.ac.at
Santner
B
B
Rabenau
H
H
Berger
A
A
Vince
A
A
Lewinski
C
C
Weber
B
B
Pierer
K
K
Stuenzner
D
D
Marth
E
E
Doerr
H W
HW
D004770:Enterovirus / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*