Further observations on coronavirus infection of primate CNS.
Previously, we demonstrated that intracerebral (IC) inoculation of a murine coronavirus, MHV-JHM, into two species of primates can result in acute encephalomyelitis (Murray et al., 1992a). Infectious virus isolated from acutely infected animals, designated JHM-OMp1, was inoculated IC into a second group of monkeys. In this report we describe observations on the acutely infected animals and those surviving the acute infection were sacrificed at later times post-infection. Results from dual in situ hybridization/immunohistochemistry screening of tissues show that astrocytes are target cells in white matter lesions during acute infection. In animals sacrificed 150 days post-infection, areas of demyelinated gliotic lesions, prominent in the spinal cord, were seen throughout the neuraxis. No virus products were detected in these late-infection lesions.
Murray
R S
RS
Colorado Multiple Sclerosis Center, Englewood 80110, USA.
Cai
G Y
GY
Soike
K F
KF
Cabirac
G F
GF
D000208:Acute Disease; D000818:Animals; D016645:Aotidae / Q000821:virology*; D001253:Astrocytes / Q000821:virology*; D002490:Central Nervous System / Q000473:pathology / Q000821:virology; D002555:Cerebrospinal Fluid / Q000821:virology; D003289:Convalescence; D018352:Coronavirus Infections / Q000134:cerebrospinal fluid / Q000473:pathology / Q000821:virology*; D004679:Encephalomyelitis / Q000134:cerebrospinal fluid / Q000473:pathology / Q000821:virology*; D005911:Gliosis / Q000473:pathology / Q000821:virology; D006517:Murine hepatitis virus / Q000302:isolation & purification / Q000472:pathogenicity*; D012367:RNA, Viral / Q000032:analysis; D013045:Species Specificity
D012367:RNA, Viral