Putative regulatory sequence in human papillomavirus type 16 E2 open reading frame.
A 114 bp fragment of the human papillomavirus type 16 (HPV16) E2 open reading frame (nt. 3142-3255) containing a putative estrogen responsive element (ERE) was amplified and cloned into pBLCAT2 plasmid in both sense (p159-4) and anti-sense (p164) orientation. The plasmids were transfected into human breast-cancer cell line MCF-7 containing estrogen receptor and the cultures were kept in the presence or absence of beta-estradiol. The chloramphenicol acetyltransferase (CAT) activity was not influenced by estrogen. However, a silencer effect was observed both in cultures transfected with p159-4 and p164 plasmids. We prepared and cloned synthetic fragments containing the putative ERE and failed to prove that the palindrome in the putative ERE was responsible for the silencer activity.
Kitasato
H
H
Department of Virology, Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
Tardy-Kadlec
A
A
Smahel
M
M
Tykva
R
R
Vonka
V
V
D001483:Base Sequence; D001943:Breast Neoplasms; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D016384:Consensus Sequence; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D017930:Genes, Reporter; D006801:Humans; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D011960:Receptors, Estrogen / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured
D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D004958:Estradiol; D015500:Chloramphenicol O-Acetyltransferase