Further analysis of the phenotype and distribution of tumor cells in sporadic B-cell and T-cell lymphomas in the lymph node and spleen of cattle.
Immunohistologic studies were performed to identify the phenotype and distribution of neoplastic lymphocytes in the spleens of BLV-negative animals examined by PCR and diagnosed as having sporadic bovine leukosis. Tumor cells from three cases of sporadic bovine leukosis were identified as of B-cell lineage. Tumor cells from three additional cattle were identified as CD3+ CD4- CD8+, CD3+ CD4- CD8-, and CD3+ CD4- WC1+, respectively. The last case was diagnosed as a gamma/delta T-cell lymphoma. Differences in morphology proliferative characteristics were recognized between B- and T-cell type lymphomas. The tumor cells in B-cell type lymphoma were characterized as follows: medium or large in size, round or polymorphic nucleus with rough chromatin with some tumor cells containing a convoluted nucleus. These tumor cells of B-cell type lymphoma were present in the red pulp and periarteriolar lymphoid sheath. Tumor cells of the T-cell type lymphoma were uniformly smaller than B-cell type and present around arteries or replaced red pulp of the spleen.
Tani
K
K
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Japan.
Asahina
M
M
Wu
D L
DL
Ajito
T
T
Murakami
K
K
Goryo
M
M
Aida
Y
Y
Davis
W C
WC
Okada
K
K
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology*; D016393:Lymphoma, B-Cell / Q000145:classification / Q000473:pathology* / Q000662:veterinary*; D016399:Lymphoma, T-Cell / Q000145:classification / Q000473:pathology* / Q000662:veterinary*; D008297:Male; D013154:Spleen / Q000276:immunology / Q000473:pathology*