Effect of freeze-drying or gamma-irradiation on remodeling of tendon allograft in a rat model.
Freezing or freeze-drying and gamma-irradiation are techniques currently used for processing tendon allografts. However, it is still unknown how these processing methods affect graft remodeling. In this study, we used a rat patellar tendon transplantation model to investigate the effect of various processing methods on remodeling by quantifying loss of collagen labeled with a radioactive isotope. The grafts were divided into the following four groups according to the processing method: fresh-frozen, freeze-dried, fresh-frozen and gamma-irradiated, or freeze-dried and gamma-irradiated. The percentage of donor collagen, calculated from hydroxyproline content and radioactivity level, was used as an indicator of graft remodeling. At 2 weeks, the level of donor collagen in the fresh-frozen group was 62%; in the freeze-dried group, 59%; in the fresh-frozen and irradiated group, 57%; and in the freeze-dried and irradiated group, 44%. At 4 weeks, the percentage of donor collagen remaining in grafts decreased to 38% in the fresh-frozen group, 19% in the freeze-dried group, 27% in the fresh-frozen and irradiated group, and 12% in the freeze-dried and irradiated group. Finally, at 12 weeks, the levels were 19% in the fresh-frozen group, 20% in the freeze-dried group, 15% in the fresh-frozen and irradiated group, and 6% in the freeze-dried and irradiated group. The percentages of donor collagen in the freeze-dried and the fresh-frozen and irradiated groups were significantly lower than that in the fresh-frozen group at 4 weeks. The values for the freeze-dried and irradiated group were significantly lower than those for the fresh-frozen and irradiated group at 4 and 12 weeks. These data suggest that freeze-drying, freeze-drying followed by gamma-irradiation, and fresh-freezing followed by gamma-irradiation temporarily accelerate graft remodeling.
Toritsuka
Y
Y
Department of Orthopaedic Surgery, Osaka University Medical School, Suita, Japan.
Shino
K
K
Horibe
S
S
Nakamura
N
N
Matsumoto
N
N
Ochi
T
T
D000818:Animals; D003094:Collagen / Q000378:metabolism; D005612:Freeze Drying; D005720:Gamma Rays; D006909:Hydroxyproline / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D013710:Tendons / Q000473:pathology / Q000528:radiation effects* / Q000637:transplantation*; D014019:Tissue Donors; D014184:Transplantation, Homologous
D003094:Collagen; D006909:Hydroxyproline