Essential role of endogenously synthesized tylosin for induction of ermSF in Streptomyces fradiae.
We compared ermSF induction in wild-type Streptomyces fradiae NRRL B-2702 and that in GS-14, a tylA mutant which cannot synthesize tylosin. Our findings suggest that (i) endogenously synthesized tylosin plays an obligatory role in ermSF induction and (ii) tylosin, or a biosynthetic intermediate beyond tylactone, has an "autocrine" function that induces ErmSF synthesis, thereby enabling S. fradiae to resist higher levels of tylosin.
Memili
E
E
Pharmacology Department, University of Wisconsin Medical School, Madison 53706, USA.
Weisblum
B
B
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D002478:Cells, Cultured; D004352:Drug Resistance, Microbial; D008780:Methyltransferases / Q000096:biosynthesis*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D015645:Tylosin / Q000096:biosynthesis* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase; D015645:Tylosin