title: alpha3beta1 Integrin is required for normal development of the epidermal basement membrane.
abstract: Integrins alpha3beta1 and alpha6beta4 are abundant receptors on keratinocytes for laminin-5, a major component of the basement membrane between the epidermis and the dermis in skin. These integrins are recruited to distinct adhesion structures within keratinocytes; alpha6beta4 is present in hemidesmosomes, while alpha3beta1 is recruited into focal contacts in cultured cells. To determine whether differences in localization reflect distinct functions of these integrins in the epidermis, we studied skin development in alpha3beta1-deficient mice. Examination of extracellular matrix by immunofluorescence microscopy and electron microscopy revealed regions of disorganized basement membrane in alpha3beta1-deficient skin. Disorganized matrix was first detected by day 15.5 of embryonic development and became progressively more extensive as development proceeded. In neonatal skin, matrix disorganization was frequently accompanied by blistering at the dermal-epidermal junction. Laminin-5 and other matrix proteins remained associated with both the dermal and epidermal sides of blisters, suggesting rupture of the basement membrane itself, rather than detachment of the epidermis from the basement membrane as occurs in some blistering disorders such as epidermolysis bullosa. Consistent with this notion, primary keratinocytes from alpha3beta1-deficient skin adhered to laminin-5 through alpha6 integrins. However, alpha3beta1-deficient keratinocytes spread poorly compared with wild-type cells on laminin-5, demonstrating a postattachment requirement for alpha3beta1 and indicating distinct roles for alpha3beta1 and alpha6beta4. Our findings support a novel role for alpha3beta1 in establishment and/or maintenance of basement membrane integrity, while alpha6beta4 is required for stable adhesion of the epidermis to the basement membrane through hemidesmosomes.
authors.affiliation: Center for Cancer Research, and Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: DiPersio
authors.forename: K M
authors.initials: KM
authors.lastname: Hodivala-Dilke
authors.forename: R
authors.initials: R
authors.lastname: Jaenisch
authors.forename: J A
authors.initials: JA
authors.lastname: Kreidberg
authors.forename: R O
authors.initials: RO
authors.lastname: Hynes
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000954:Antigens, Surface / Q000378:metabolism; D001485:Basement Membrane / Q000648:ultrastructure*; D001768:Blister / Q000235:genetics; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D004817:Epidermis / Q000254:growth & development*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D039201:Integrin alpha3beta1; D039161:Integrin alpha6beta4; D016023:Integrins / Q000378:metabolism / Q000502:physiology*; D015603:Keratinocytes / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D000097794:Kalinin
chemical_list: D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D003094:Collagen; D016326:Extracellular Matrix Proteins; D039201:Integrin alpha3beta1; D039161:Integrin alpha6beta4; D016023:Integrins; D008562:Membrane Glycoproteins; D000097794:Kalinin; C029967:nidogen