title: Prophylactic effects of isometamidium- and ethidium-sustained release devices against Trypanosoma congolense in cattle.
abstract: Two successive experiments were carried out in which three cows were treated by intramuscular injection of either 0.5 mg/kg isometamidium or 1 mg/kg ethidium and compared with another group of three cows which received a subcutaneously implanted sustained release device (SRD) containing the same dose of drug. The prophylactic effect of both drug formulations was evaluated by exposing the animals at monthly intervals to Glossina morsitans morsitans infected with Trypanosoma congolense. The average protection period using the isometamidium- and the ethidium-SRD was extended by a factor of 3.2 and 2.8, respectively in comparison with the intramuscular injection of the drugs. In the analysis of isometamidium concentrations in the serum of the animals using a competitive drug-ELISA the drugs remained present for much longer periods in the sera of the implanted animals than in those of the intramuscularly treated cattle. The animals were still protected, however, a long time after the disappearance of detectable drug levels in the serum. No difference in drug sensitivity could be observed, when breakthrough isolates were compared from animals which received the ethidium-SRD and those treated intramuscularly, although a slight loss sensitivity occurred in the breakthrough isolates as compared to the parent trypanosome population.
authors.affiliation: Institute of Tropical Medicine, Antwerpen, Belgium. sgeerts@itg.be
authors.forename: S
authors.initials: S
authors.lastname: Geerts
authors.forename: P
authors.initials: P
authors.lastname: Kageruka
authors.forename: R
authors.initials: R
authors.lastname: De Deken
authors.forename: J R
authors.initials: JR
authors.lastname: Brandt
authors.forename: J M
authors.initials: JM
authors.lastname: Kazadi
authors.forename: B
authors.initials: B
authors.lastname: Diarra
authors.forename: M C
authors.initials: MC
authors.lastname: Eisler
authors.forename: Y
authors.initials: Y
authors.lastname: Lemmouchi
authors.forename: E
authors.initials: E
authors.lastname: Schacht
authors.forename: P H
authors.initials: PH
authors.lastname: Holmes
mesh_terms: D000818:Animals; D002417:Cattle; D004996:Ethidium / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D005260:Female; D015918:Infusion Pumps, Implantable; D008826:Microbial Sensitivity Tests; D010617:Phenanthridines / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D014348:Trypanosoma congolense / Q000187:drug effects*; D014353:Trypanosomiasis, African / Q000097:blood / Q000188:drug therapy* / Q000517:prevention & control*
chemical_list: D010617:Phenanthridines; D014344:Trypanocidal Agents; C000702:isometamidium chloride; D004996:Ethidium