Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter strain 90 belonging to DNA group 10.
Water-soluble lipopolysaccharide (phenol/water extraction) isolated from Acinetobacter strain 90, which belongs to DNA group 10, was hydrolysed with 1% acetic acid, ultracentrifuged, and water-soluble products finally eluted from a Sephadex G-50 column. The major fraction, a polysaccharide, contained D-Gal, D-GlcNAc, D-GalNAc, and 4,6-dideoxy-4-[(R)-3-hydroxybutyramido]-D-galactose (Fuc4NBuOH). The polysaccharide was characterised by means of monosaccharide analyses, Smith-degradation, N-deacetylation/deamination, and NMR studies, and was shown to have a branched pentasaccharide repeating unit. [structure in text] This structure was specifically recognised in western blots and enzyme immunoassays by polyclonal rabbit antisera.
Haseley
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Division of Medical and Biochemical Microbiology, Research Centre Borstel, Centre for Medicine and Biosciences, Germany.
Holst
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O
Brade
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D000150:Acinetobacter / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D004269:DNA, Bacterial / Q000737:chemistry*; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011817:Rabbits; D012995:Solubility
D004269:DNA, Bacterial; D007106:Immune Sera; D019081:O Antigens; C106365:O-antigen, Acinetobacter strain 90