title: Activation-induced expression of human programmed death-1 gene in T-lymphocytes.
abstract: The Programmed Death-1 (PD-1) gene is a member of the immunoglobulin superfamily of genes. Murine PD-1 mRNA expression has been shown to correlate with activation-induced apoptosis in a mouse T-cell hybridoma cell line and in murine thymocytes. Here we report that expression of the human homolog, hPD-1, seems to correlate with activation of T lymphocytes rather than apoptosis. We observed a time-dependent upregulation of hPD-1 mRNA and protein levels in Jurkat cells during phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA)-induced differentiation. Human PD-1 protein was also induced during lectin-stimulated activation of human peripheral blood mononuclear cells. Additionally, TPA stimulation of Jurkat cells induces tyrosine phosphorylation of hPD-1, putatively on its cytoplasmic tail signal transduction motif. These data suggest a role for hPD-1 during activation and differentiation of T-lymphocytes.
authors.affiliation: Department of Pathology, New York Medical College, Elmsford 10523, USA. Pumba2c@aol.com
authors.forename: R
authors.initials: R
authors.lastname: Vibhakar
authors.forename: G
authors.initials: G
authors.lastname: Juan
authors.forename: F
authors.initials: F
authors.lastname: Traganos
authors.forename: Z
authors.initials: Z
authors.lastname: Darzynkiewicz
authors.forename: L R
authors.initials: LR
authors.lastname: Finger
mesh_terms: D015703:Antigens, CD; D000954:Antigens, Surface / Q000096:biosynthesis*; D051017:Apoptosis Regulatory Proteins; D002454:Cell Differentiation; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D010766:Phosphorylation; D061026:Programmed Cell Death 1 Receptor; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D051017:Apoptosis Regulatory Proteins; C105992:PDCD1 protein, human; C491383:Pdcd1 protein, mouse; D061026:Programmed Cell Death 1 Receptor; D014443:Tyrosine; D013755:Tetradecanoylphorbol Acetate