[A cytofluorometric study of the stability of secondary DNA structure in the epithelial cells in endometrial hyperplasia and cancer].
Studies were performed on epithelial cells obtained from uterine cavity in 49 women. All cases were subdivided into four groups; unaltered endometrium-9, glandular endometrial hyperplasia-10, atypical hyperplasia-4 and endometrial adenocarcinoma-30. For fluorimetric evaluation of single- and double-strand DNA specimens were dyed with acridine orange, which is known to produce red fluorescence in combination with double-strand acids. Values of coefficients alpha for single and double-strand DNA after acid hydrolysis were much higher than those before acid treatment. This study provides a deeper insight into the relation between DNA lesions and malignancy.
Ganina
K P
KP
Boĭko
Iu V
IuV
D000230:Adenocarcinoma / Q000648:ultrastructure*; D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure; D004247:DNA / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000648:ultrastructure*; D004277:DNA, Single-Stranded / Q000648:ultrastructure*; D004355:Drug Stability; D004714:Endometrial Hyperplasia / Q000473:pathology*; D016889:Endometrial Neoplasms / Q000648:ultrastructure*; D004717:Endometrium / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D004247:DNA
Tsitofliuorometricheskoe issledovanie stabil'nosti vtorichnoĭ struktury DNK épitelial'nykh kletok pri giperplazii i rake éndometriia.