Three generations of high-density lipoprotein cholesterol assays compared with ultracentrifugation/dextran sulfate-Mg2+ method.
We report on the analytical performance of three generations of HDL-cholesterol assays: phosphotungstic acid/Mg2+, Spinpro, and a homogeneous method, N-geneous. The run-to-run imprecision (CV) of all assays was < or = 4.9%, and all results correlated highly with those of a modified reference procedure (r > or = 0.96). At triglycerides concentrations < 4000 mg/L, these field methods showed an acceptable systematic error (y = 1.12x - 47, 1.05x - 23, and 0.96x + 8 for the phosphotungstate, Spinpro, and N-geneous assays, respectively), and the total error of the field methods met the current National Cholesterol Education Program (NCEP) performance goal of < or = 22%. Regression analyses of results for samples with triglycerides > 4000 mg/L produced the following results for the above respective assays: y = 1.08x - 4.2, 1.02x + 3.6, and 0.85x + 108. In this hypertriglyceridemic group, only the N-geneous assay (at an HDL-cholesterol content of 240 mg/L) had a total error (35%) that exceeded the NCEP limit. Bilirubin and ascorbate produced a negative interference with the phosphotungstate and Spinpro assays but had little effect on the N-geneous assay.
Harris
N
N
Department of Laboratory Medicine and Pathology, Children's Hospital, Boston, MA 02115, USA. harris n@al.tch.harvard.edu
Galpchian
V
V
Thomas
J
J
Iannotti
E
E
Law
T
T
Rifai
N
N
D001205:Ascorbic Acid / Q000097:blood; D001663:Bilirubin / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood*; D016264:Dextran Sulfate; D005188:False Negative Reactions; D005215:Fasting; D006801:Humans; D008274:Magnesium; D010772:Phosphotungstic Acid; D011786:Quality Control; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation
D008076:Cholesterol, HDL; D014280:Triglycerides; D010772:Phosphotungstic Acid; D016264:Dextran Sulfate; D008274:Magnesium; D001205:Ascorbic Acid; D001663:Bilirubin