title: The manganese superoxide dismutase gene of Drosophila: structure, expression, and evidence for regulation by MAP kinase.
abstract: The gene encoding manganese superoxide dismutase (MnSOD) from Drosophila melanogaster has been isolated and its expression has been studied. In contrast to several mammalian MnSOD genes, the Drosophila gene contains a single intron and is transcribed into a single 0.8-kb transcript. Whole-mount in situ hybridization reveals extensive transcript accumulation in ovarian nurse cells and a heavy maternal contribution to the early embryo. Larval imaginal discs are enriched with MnSOD transcripts relative to other larval tissues, further suggesting a possible relationship between high MnSOD expression and mitotic activity. The 5'-upstream region contains several well-known regulatory elements including metal response, antioxidant response, and xenobiotic response elements (MRE, ARE, and XRE, respectively), sites for activator protein-1 (AP-1), nuclear factor-kappaB (NF-kappaB), adenosine 3',5'-cyclic monophosphate regulator binding element factor (CREB), as well as classic TATA and CAAT boxes. That MnSOD expression in Drosophila is regulated in part by the transcription factor AP-1 via the MAP kinase signal transduction pathway is suggested by experiments which show that a hypomorphic mutation of the MAP kinase-encoding rolled gene substantially reduces levels of MnSOD transcripts and correlates with reduced resistance to oxidative stress in rolled mutants.
authors.affiliation: Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Duttaroy
authors.forename: T
authors.initials: T
authors.lastname: Parkes
authors.forename: P
authors.initials: P
authors.lastname: Emtage
authors.forename: K
authors.initials: K
authors.lastname: Kirby
authors.forename: G L
authors.initials: GL
authors.lastname: Boulianne
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: A J
authors.initials: AJ
authors.lastname: Hilliker
authors.forename: J P
authors.initials: JP
authors.lastname: Phillips
mesh_terms: D000818:Animals; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases; D015971:Gene Expression Regulation, Enzymologic; D017344:Genes, Insect; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000235:genetics*; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D018808:Transcription Factor AP-1; D013482:Superoxide Dismutase; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; C086795:rl protein, Drosophila; D010269:Paraquat