Immunohistochemical aspects of chromogranins in endocrine cells of the duodenal mucosa in some primates.
Endocrine cells in the duodenal mucosa of some primates (Cercopithecus aetiops, Macaca cinomolgus-nemestrina and Macaca rhesus) have been studied with immunohistochemical methods for chromogranins. Material sampling immediately after death (by carotid bleeding concomitantly with formalin 10% perfusion) was helpful in the study of endocrine cells. The location and number of endocrine cells of the duodenal mucosa of the primates under study are generally similar to those of humans. The use of an antibody cocktail against all three Cgs/Sgs appears to be the method of choice to identify neuroendocrine granule-containing cells. When comparing the results of this study with those obtained by us in a previous work by silver staining, the Grimelius technique is recommended together with the immunohistochemical techniques for chromogranins in the practice of the usual diagnosis of the endocrine pathology.
Radu
I
I
Faculty of Medicine, Department of Histology, Craiova, Romania.
D000818:Animals; D002522:Chlorocebus aethiops / Q000033:anatomy & histology / Q000378:metabolism*; D002864:Chromogranins / Q000032:analysis*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000166:cytology; D008251:Macaca / Q000033:anatomy & histology / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000166:cytology / Q000378:metabolism*
D002864:Chromogranins