Is an ATPase involved in uncoating of plasma membrane adaptor complex AP-2?
The coat of clathrin-coated vesicles mostly consists of clathrin and adaptor complexes AP-1 or AP-2. Clathrin is released from the vesicles in an ATP-dependent fashion prior to their fusion with endosomes. In the present study we found that ATP strongly inhibits in vitro binding of cytosolic AP-2 to membranes of stripped vesicles, and promotes the release of endogenous AP-2 from clathrin-deprived coated vesicles. Both effects required hydrolysis of ATP. In contrast, binding of AP-1 to stripped vesicles was not affected by ATP, but was enhanced by GTP-gamma-S. These results point to an ATPase that promotes the release of AP-2 from clathrin-coated vesicles.
Sosa
M A
MA
Instituto de Histologia y Embriologia, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
Bertini
F
F
von Figura
K
K
Hille-Rehfeld
A
A
D000214:Acyl Coenzyme A / Q000378:metabolism*; D033942:Adaptor Proteins, Vesicular Transport; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism*; D018699:Coated Vesicles / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006868:Hydrolysis; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects
D000214:Acyl Coenzyme A; D033942:Adaptor Proteins, Vesicular Transport; D002966:Clathrin; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; C106238:formyl-coenzyme A; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases