title: On-line micellar electrokinetic chromatography-electrospray ionization mass spectrometry using anodically migrating micelles.
abstract: On-line micellar electrokinetic chromatography (MEKC)-electrospray ionization mass spectrometry (ESIMS) is demonstrated for the analysis of chlorotriazine herbicides and barbiturates. In this study, the micellar velocity is directly manipulated by the adjustment of electroosmosis rather than the electrophoretic velocity of the micelle. The electroosmotic flow is adjusted against the electrophoretic velocity of the micelle by changing the solution pH in MEKC. The elimination of MEKC surfactant introduction into ESIMS is achieved with an anodically migrating micelle, moving away from the electrospray interface. The effects of moving surfactant boundary in the MEKC capillary on separation efficiency and resolution of triazine herbicides and barbiturates are investigated. The mass detection of herbicides and barbiturates sequentially eluted from the MEKC capillary is acquired using the positive and negative electrospray modes, respectively.
authors.affiliation: Department of Chemistry, USDOE, Iowa State University, Ames 50011, USA.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: A K
authors.initials: AK
authors.lastname: Harrata
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
mesh_terms: D001463:Barbiturates / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002845:Chromatography / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D006540:Herbicides / Q000032:analysis; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D008823:Micelles; D014227:Triazines
chemical_list: D001463:Barbiturates; D006540:Herbicides; D008823:Micelles; D014227:Triazines