title: Urinary biotinidase and alanine excretion in patients with insulin-dependent diabetes mellitus.
abstract: Twenty-four-hour urine specimens from 21 juvenile insulin-dependent diabetics and 10 healthy controls were compared with respect to biotinidase activity and alanine content. Urinary biotinidase activity was analysed by a newly developed high-performance liquid chromatography (HPLC) method. It was found that the excretion of biotinidase in urine was elevated in diabetics (7.02 mU/d; p < 0.005) as compared with controls (not detectable). Alanine excretion was also found to increase (p < 0.01) in diabetics. Biotinidase excretion in diabetics was correlated with alanine excretion (rS = 0.667; p < 0.01), but not with protein, albumin or N-acetyl-beta-glucosaminidase excretion. The simultaneous elevation of urinary biotinidase and alanine excretion in juvenile diabetics suggests that changes in kidney metabolism arise in the early stages of diabetes.
authors.affiliation: Endocrine and Metabolism Research Laboratory, National Children's Medical Research Center, Tokyo, Japan.
authors.forename: E A
authors.initials: EA
authors.lastname: Terentyeva
authors.forename: K
authors.initials: K
authors.lastname: Hayakawa
authors.forename: A
authors.initials: A
authors.lastname: Tanae
authors.forename: N
authors.initials: N
authors.lastname: Katsumata
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: I
authors.initials: I
authors.lastname: Hibi
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000293:Adolescent; D000328:Adult; D000409:Alanine / Q000652:urine*; D000419:Albuminuria / Q000201:enzymology / Q000652:urine; D000581:Amidohydrolases / Q000652:urine*; D043523:Biotinidase; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology* / Q000378:metabolism / Q000652:urine*; D003928:Diabetic Nephropathies / Q000201:enzymology / Q000209:etiology / Q000652:urine; D005260:Female; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011507:Proteinuria / Q000201:enzymology / Q000652:urine
chemical_list: D000118:Acetylglucosaminidase; D000581:Amidohydrolases; D043523:Biotinidase; D000409:Alanine