Histochemical study of lectin binding in the major salivary glands of adult fallow-deer (Dama dama L.).
The sugar residues in glycoconjugates present in the parotid and mandibular glands of the adult fallow-deer were detected and characterized by using a battery of eight different lectin-horseradish peroxidase conjugates. In some cases a treatment with sialidase preceded the lectin staining. Parotid secretory cells produced glycoconjugates with N-acetylgalactosamine, N-acetylglucosamine and mannose residues. Mucous acinar cells were the most reactive sites of the mandibular gland and contained conspicuous quantities of oligosaccharides with terminal sialic acid radicals. Galactosil-(beta 1-->3)N-acetylgalactosamine was the most abundant penultimate sugar linked to N-acetylneuraminic acid. Mandibular mucous cells also presented N-acetylglucosamine and sialylated components with the terminal dimer sialic acid-N-acetylgalactosamine. Demilunar cells contained glycoconjugates with fucose and mannose residues. The apical surface of duct cells was stained by all the lectins.
Pedini
V
V
Department of Anatomy for Domestic Animals, Faculty of Veterinary Medicine, Matelica, Italy.
Ceccarelli
P
P
Gargiulo
A M
AM
Dall'Aglio
C
C
D000818:Animals; D001665:Binding Sites; D003670:Deer; D005260:Female; D006651:Histocytochemistry; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D010306:Parotid Gland / Q000166:cytology / Q000378:metabolism; D012469:Salivary Glands / Q000166:cytology* / Q000378:metabolism*
D037102:Lectins