Binding and distribution of Na2B12H11SH on cellular and subcellular level in tumor tissue of glioma patients in boron neutron capture therapy.
To determine binding and distribution of Na2B12H11SH (BSH) in glioma tissue in case of boron neutron capture therapy, an antibody to this compound was produced and used in immunohistochemical investigations. It is possible to trace BSH in immunohistochemistry, because BSH is firmly bound to the glioma tissue. The antibody against BSH is specific for that antigen, as tumor tissue from patients without BSH administration did not stain. In areas of healthy brain from BSH infused patients, no staining of tissue was detectable. In tumor tissues, BSH is presenting as a strong staining in cytoplasm and nucleus areas.
Otersen
B
B
Department of Chemistry, University of Bremen, Germany.
Haritz
D
D
Grochulla
F
F
Bergmann
M
M
Sierralta
W
W
Gabel
D
D
D000906:Antibodies; D001665:Binding Sites; D001894:Borohydrides / Q000032:analysis / Q000493:pharmacokinetics*; D016754:Boron Neutron Capture Therapy; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy* / Q000601:surgery; D005910:Glioma / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy* / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D013347:Subcellular Fractions / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D000906:Antibodies; D001894:Borohydrides; D013438:Sulfhydryl Compounds; C014651:mercaptoundecahydrododecaborate