A rapid urease test for presumptive identification of Cryptococcus neoformans.
A rapid method to evidence urease activity is described. Urea hydrolysis and consequent production ammonia are detected by a chemical reaction producing a blue phenol compound (indophenol blue). Three hundred and three yeast were tested. Out of 107 urease-positive organisms detected by Christensen's Urea Agar Test (CUAT) 102 were positive by our method. No false negatives were observed by this method when testing 87 Cryptococcus strains. Ths practical screening test for presumptive identification of Cryptococcus neoformans is simple, unaffected by pH changes and requires 15 minutes to be performed.
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Departamento de Micologia, Instituto Nacional de Microbiologia Dr. Carlos Malbran, Buenos Aires, Argentina.
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D019411:Clinical Laboratory Techniques / Q000379:methods*; D003453:Cryptococcosis / Q000175:diagnosis*; D003455:Cryptococcus neoformans / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005188:False Negative Reactions; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013997:Time Factors; D014510:Urease / Q000378:metabolism*
D003470:Culture Media; D014510:Urease