title: Voltammetric detection of nitric oxide (NO) in the rat brain: its variations throughout the sleep-wake cycle.
abstract: A sensor allowing the specific detection of nitric oxide (NO) is reported. Together with differential pulsed voltammetry, it allows the detection of a 650 mV signal either in NO solutions or in the rat frontal cortex. The intraperitoneal (i.p.) administration of a NO donor (S-nitrosoglutathione, 20 mg/kg i.p.) increases the signal height (+30%) while that of a nitric oxide synthase (NOS) inhibitor like L-nitro-arginine-p-nitro-anilide (100 mg/kg i.p.), produces its complete disappearance in the cortex of anesthetized rats. These results suggest that the 650 mV signal might be NO-dependent. Some other NOS inhibitors have been found either inefficient (L-nitro-arginine-methyl-ester) or partially efficient (7-nitro-indazole) on the signal height. In freely moving rats, also equipped with polygraphic electrodes, the signal measured in the frontal cortex exhibits the highest height during waking. It decreases during slow-wave sleep (-6%) and paradoxical sleep (-9%).
authors.affiliation: Department of Experimental Medicine, INSERM-U52 and CNRS-ERS5645, Lyon, France.
authors.forename: S
authors.initials: S
authors.lastname: Burlet
authors.forename: R
authors.initials: R
authors.lastname: Cespuglio
mesh_terms: D000204:Activity Cycles; D000704:Analysis of Variance; D000813:Anilides; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004563:Electrochemistry / Q000379:methods; D005625:Frontal Lobe / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D009603:Nitroso Compounds / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D026422:S-Nitrosoglutathione; D012890:Sleep / Q000502:physiology*; D014851:Wakefulness / Q000502:physiology*
chemical_list: D000813:Anilides; D009603:Nitroso Compounds; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; C080840:N(G)-nitroarginine-4-nitroanilide; D001120:Arginine; D005978:Glutathione; D019331:NG-Nitroarginine Methyl Ester