Surfactant protein-B and lung function in surfactant-treated preterm lambs.
We asked if the amount of SP-B (range 37-410 micrograms/ml) in surfactants used to treat preterm lambs at 123 days of gestation correlated with postnatal lung function or the SP-B content of surfactant recovered by alveolar washes after 10 h ventilation. Ventilation was initiated using a low tidal volume strategy to minimize early lung injury. There were small increases in compliance for the lambs treated with surfactants containing more than 37 micrograms/ml SP-B and an increased lung volume for lambs treated with a surfactant containing 385 micrograms/ml/ml relative to the surfactant containing 37 micrograms/ml SP-B. The amount of SP-B in the surfactant used for treatment correlated linearly with the amount of SP-B in the surfactant recovered from the lambs (r = 0.95, p < 0.001). Albumin leak from the vasculature to the airspace was low as was total protein in alveolar washes, indicating minimal lung injury. The SP-B content of surfactant (from 37 to 410 micrograms/ml) had minimal effects on postnatal lung function over a 10-hour study period in lambs ventilated in a manner to minimize lung injury.
Rebello
C M
CM
Harbor-UCLA Medical Center, Department of Pediatrics, Torrance, USA.
Ikegami
M
M
Hernandez
R E
RE
Jobe
A H
AH
D001688:Biological Products; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D011510:Proteolipids / Q000032:analysis*; D011663:Pulmonary Surfactants / Q000032:analysis* / Q000737:chemistry / Q000627:therapeutic use*; D012131:Respiratory Insufficiency / Q000188:drug therapy / Q000662:veterinary*; D015656:Respiratory Mechanics / Q000187:drug effects*
D001688:Biological Products; D011510:Proteolipids; D011663:Pulmonary Surfactants; C072197:beractant