title: Purification and characterization of an invertase from Candida utilis: comparison with natural and recombinant yeast invertases.
abstract: A periplasmic invertase from the yeast Candida utilis was purified to homogeneity from cells fully derepressed for invertase synthesis. The enzyme was purified by successive Sephacryl S-300, and affinity chromatography and shown to be a dimeric glycoprotein composed of two identical monomer subunits with an apparent molecular mass of 150 kDa. After EndoH treatment, the deglycosylated protein showed an apparent molecular weight of 60 kDa. The apparent K(m) values for sucrose and raffinose were 11 and 150 mM, respectively, similar to those reported in Saccharomyces cerevisiae. The range of optimum temperature was 60-75 degrees C. The optimum pH was 5.5 and the enzyme was stable over pH range 3-6.
authors.affiliation: Bioindustry Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: F P
authors.initials: FP
authors.lastname: Chávez
authors.forename: L
authors.initials: L
authors.lastname: Rodriguez
authors.forename: J
authors.initials: J
authors.lastname: Díaz
authors.forename: J M
authors.initials: JM
authors.lastname: Delgado
authors.forename: J A
authors.initials: JA
authors.lastname: Cremata
mesh_terms: D015139:Blotting, Southern; D002175:Candida / Q000737:chemistry / Q000201:enzymology* / Q000254:growth & development; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006031:Glycosylation; D007700:Kinetics; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013379:Substrate Specificity; D013696:Temperature; D043324:beta-Fructofuranosidase
chemical_list: D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005947:Glucose; D005990:Glycerol