Genes encoding giant danio and golden shiner ependymin.
Ependymin (EPN) is a brain glycoprotein that functions as a neurotrophic factor in optic nerve regeneration and long-term memory consolidation in goldfish. To date, true epn genes have been characterized in one order of teleost fish, Cypriniformes. In the study presented here, polymerase chain reactions were used to analyze the complete epn genes, gd (1480 bp), and sh (2071 bp), from Cypriniformes giant danio and shiner, respectively. Southern hybridizations demonstrated the existence of one copy of each gene per corresponding haploid genome. Each gene was found to contain six exons and five introns. Gene gd encodes a predicted 218-amino acid (aa) protein GD 93 percent conserved to goldfish EPN, while sh encodes a predicted 214-aa protein SH 91 percent homologous to goldfish. Evidence is presented classifying proteins previously termed "EPNs" into two major categories: true EPNs and non-EPN cerebrospinal fluid glycoproteins. Proteins GD and SH contain all the hallmark, features of true EPNs.
Adams
D S
DS
Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Kiyokawa
M
M
Getman
M E
ME
Shashoua
V E
VE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002347:Carps; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003531:Cypriniformes / Q000235:genetics*; D017931:DNA Primers; D006054:Goldfish; D008969:Molecular Sequence Data; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D009900:Optic Nerve / Q000502:physiology; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D017931:DNA Primers; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C031632:ependymins