title: Fluid shear stress increases the release of platelet derived growth factor BB (PDGF BB) by aortic endothelial cells.
abstract: OBJECTIVE
  The aim of this study was to determine the correlation between shear stress and the release of Platelet Derived Growth Factor (PDGF BB) by aortic endothelial cells.
  
  
  METHODS
  Laboratory in vitro study.
  
  
  METHODS
  Bovine aortic endothelial cells were seeded in fibronectin-coated cylinders at 1.0 x 10(6) cells/tube and allowed to reach confluence and to adhere for 48 hours. The experimental groups were subjected to nonpulsatile, laminar flow of 50, 100, 150 ml/min in polystyrene cylinders (i.d. 10 mm) of a closed circulatory loop giving a shear stress on the endothelial cells of 3, 6, 9 dyn/cm2. The control group was subjected to similar incubation conditions without flow.
  
  
  METHODS
  The release of PDGF BB by endothelial cells was measured by ELISA and Western Blot Analysis.
  
  
  RESULTS
  Shear stress increased significantly (p < 0.01) the release of PDGF BB by endothelial cells.
  
  
  CONCLUSIONS
  PDGF BB release by endothelial cells may be one of the mechanisms linking hemodynamic forces and adaptation of blood vessels wall.
authors.affiliation: I Istituto di Clinica Chirurgica, University of Rome, La Sapienza.
authors.forename: C
authors.initials: C
authors.lastname: Aromatario
authors.forename: A V
authors.initials: AV
authors.lastname: Sterpetti
authors.forename: R
authors.initials: R
authors.lastname: Palumbo
authors.forename: A L
authors.initials: AL
authors.lastname: Patrizi
authors.forename: A
authors.initials: A
authors.lastname: Di Carlo
authors.forename: P
authors.initials: P
authors.lastname: Proietti
authors.forename: M B
authors.initials: MB
authors.lastname: Guglielmi
authors.forename: A
authors.initials: A
authors.lastname: Cavallaro
authors.forename: L
authors.initials: L
authors.lastname: Santoro-D'Angelo
authors.forename: A
authors.initials: A
authors.lastname: Cucina
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D018495:Fluid Shifts; D066298:In Vitro Techniques; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D012212:Rheology
chemical_list: D017077:Culture Media, Conditioned; D010982:Platelet-Derived Growth Factor