[The effect of a protein concentrate from milk whey and its fractions on the macromolecular permeability of the intestinal barrier in rats with experimental food anaphylaxis].
Rats received dietary supplement of milk whey concentrate (KSP) being manufactured from skimmed milk by addition of apple pectin (Pec), KSP ultrafiltrated fractions and pure Pec during 21 day. A part of animals were sensitized i/p with chicken ovalbumin and finally subjected to systemic anaphylaxis (SA) by i/v antigen challenge. Gastrointestinal barrier macromolecular permeability (MP) for polyethylene glycol 4000 (PEG-4000) was measured by its urinary excretion after an intragastric load. MP increased significantly in nonsensitized rats after feeding with KSP and its high molecular (HM) fraction but not after feeding with low molecular (LM) KSP fraction compared to NaCl fed group. Feeding with Pec dramatically (5-fold) increased permeability. On the contrary the permeability decreased in sensitized rats subjected to SA when fed with KSP, its LM and particularly HM fraction the later group permeability being normalized down to normal value found in nonsensitized animals. It's concluded that KSP whey protein may be recommended for incorporation into specialized foods for patients with impaired gastrointestinal function.
Gmoshinskiĭ
I V
IV
Mazo
V K
VK
Zorin
S N
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D000707:Anaphylaxis / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D005512:Food Hypersensitivity / Q000188:drug therapy* / Q000378:metabolism; D007114:Immunization / Q000379:methods; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D046911:Macromolecular Substances; D008297:Male; D008894:Milk Proteins / Q000627:therapeutic use*; D008970:Molecular Weight; D010368:Pectins / Q000627:therapeutic use; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D000067816:Whey Proteins
D046911:Macromolecular Substances; D008894:Milk Proteins; D000067816:Whey Proteins; D011092:Polyethylene Glycols; D010368:Pectins
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