Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification.
DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence variation among these pig-derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically distinct from P. carinii isolated from other mammalian host species.
Wakefield
A E
AE
Department of Paediatrics, John Radcliffe Hospital, Oxford, UK.
Keely
S P
SP
Stringer
J R
JR
Christensen
C B
CB
Ahrens
P
P
Peters
S E
SE
Bille-Hansen
V
V
Henriksen
S A
SA
Jorsal
S E
SE
Settnes
O P
OP
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000032:analysis* / Q000737:chemistry; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D006801:Humans; D008168:Lung / Q000473:pathology / Q000821:virology*; D051379:Mice; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D011020:Pneumonia, Pneumocystis / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000235:genetics; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D012313:RNA