title: Uracil DNA glycosylase specifically interacts with Vpr of both human immunodeficiency virus type 1 and simian immunodeficiency virus of sooty mangabeys, but binding does not correlate with cell cycle arrest.
abstract: The Vpr protein encoded by human immunodeficiency virus type 1 (HIV-1) is important for growth of virus in macrophages and prevents infected cells from passing into mitosis (G2 arrest). The cellular target for these functions is not known, but Vpr of HIV-1 and the related Vpr from simian immunodeficiency virus of sooty mangabeys (SIV(SM)) bind the DNA repair enzyme UNG, while the Vpx protein of SIV(SM) does not. Nonetheless, a mutational analysis of Vpr showed that binding to UNG is neither necessary nor sufficient for the effect of Vpr on the cell cycle.
authors.affiliation: Laboratoire de Génétique Moléculaire des Interactions Protéiques, INSERM U332, ICGM, Université Paris V, France.
authors.forename: L
authors.initials: L
authors.lastname: Selig
authors.forename: S
authors.initials: S
authors.lastname: Benichou
authors.forename: M E
authors.initials: ME
authors.lastname: Rogel
authors.forename: L I
authors.initials: LI
authors.lastname: Wu
authors.forename: M A
authors.initials: MA
authors.lastname: Vodicka
authors.forename: J
authors.initials: J
authors.lastname: Sire
authors.forename: R
authors.initials: R
authors.lastname: Benarous
authors.forename: M
authors.initials: M
authors.lastname: Emerman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle; D016664:Cercocebus / Q000382:microbiology; D045647:DNA Glycosylases; D016195:G2 Phase; D016336:Gene Products, vpr / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D011485:Protein Binding; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015302:Simian Immunodeficiency Virus / Q000378:metabolism*; D051981:Uracil-DNA Glycosidase; D054334:Viral Regulatory and Accessory Proteins / Q000378:metabolism; D054325:vpr Gene Products, Human Immunodeficiency Virus
chemical_list: D016336:Gene Products, vpr; C074286:VPX protein, Human immunodeficiency virus 2; C513059:VPX protein, Simian immunodeficiency virus; D054334:Viral Regulatory and Accessory Proteins; D054325:vpr Gene Products, Human Immunodeficiency Virus; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase