title: High-performance liquid chromatographic method for an automated determination of local anaesthetics in human plasma.
abstract: A method is described that allows the rapid and precise determination of the local anaesthetics bupivacaine and etidocaine from biological fluids. This method uses a fully automated system with solid-phase extraction in combination with a column-switching technique. Both sample extraction on a LiChrocart pre-column and elution onto the analytical LiChrospher column, were performed automatically and concomitantly using conventional HPLC equipment in conjunction with an OSP-2 on-line sample preparator from Merck combined with UV detection. Recoveries were found to be 96.7 and 96.4% for 2 micrograms/ml bupivacaine and etidocaine, respectively. Lower limits of quantification were found to be 0.05 microgram/ml plasma for both of the compounds.
authors.affiliation: Outpatient Clinic, University Clinic, Kantonsspital Basel, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Drewe
authors.forename: S
authors.initials: S
authors.lastname: Rufer
authors.forename: J
authors.initials: J
authors.lastname: Huwyler
authors.forename: E
authors.initials: E
authors.lastname: Küsters
mesh_terms: D000779:Anesthetics, Local / Q000097:blood*; D001322:Autoanalysis; D002045:Bupivacaine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005041:Etidocaine / Q000097:blood*; D006801:Humans; D011786:Quality Control
chemical_list: D000779:Anesthetics, Local; D005041:Etidocaine; D002045:Bupivacaine