Peptidoglycan composition of vancomycin-resistant Enterococcus faecium.
Muropeptide composition of peptidoglycan isolated from isogenic vancomycin-resistant and sensitive Enterococcus faecium strains was analyzed by reverse-phase high-performance liquid chromatography combined with amino acid and fast atom bombardment mass spectrometric analyses. Peptidoglycan of the sensitive and resistant strains was the same and was composed of tri- and tetrapeptides stem peptide subunits with or without aspartate or asparagine substitutions on the epsilon-amino group of the lysine residue. Thus, the synthesis of lactate-terminating peptidoglycan precursors in vancomycin-resistant E. faecium did not affect the chemical composition of peptidoglycan.
de Jonge
B L
BL
Laboratory of Microbiology, Rockefeller University, New York, New York 10021, USA.
Gage
D
D
Handwerger
S
S
D000596:Amino Acids / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics; D007773:Lactates / Q000378:metabolism; D008970:Molecular Weight; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014640:Vancomycin / Q000494:pharmacology*
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D007773:Lactates; D010457:Peptidoglycan; D014640:Vancomycin; D002267:Muramoylpentapeptide Carboxypeptidase