title: Evidence for local production of acute phase response apolipoprotein serum amyloid A in Alzheimer's disease brain.
abstract: Acute phase serum amyloid A (A-apoSAA), but not constitutive apoSAA (C-apoSAA), was identified by Western blotting experiments in brain protein extracts from eight of nine patients with Alzheimer's disease (AD), one with a brain tumor and one with multiple sclerosis. A-apoSAA was not detected in six subjects with Pick's or Lewy Body disease or three other non-AD brain specimens. Apolipoprotein A-I and albumin were not found in any of the brain protein extracts. A-apoSAA mRNA was detected in AD brain by reverse transcription-polymerase chain reaction (RT-PCR). These data suggest that apoSAA is locally produced in AD brain and that investigation of the neuroinflammatory effects of this injury specific apolipoprotein is warranted.
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, MA 02118, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Liang
authors.forename: J A
authors.initials: JA
authors.lastname: Sloane
authors.forename: J M
authors.initials: JM
authors.lastname: Wells
authors.forename: C R
authors.initials: CR
authors.lastname: Abraham
authors.forename: R E
authors.initials: RE
authors.lastname: Fine
authors.forename: J D
authors.initials: JD
authors.lastname: Sipe
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D001053:Apolipoproteins / Q000096:biosynthesis* / Q000378:metabolism*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001053:Apolipoproteins; D000685:Serum Amyloid A Protein