A lipoprotein secreted by cultured hepatocytes of silver or yellow eel: comparison with their plasma lipoproteins.
Cultured hepatocytes of silver eel actively secreted only chylomicron-like lipoprotein. The rate of secretion per mg cellular protein per 24 hr was 2.2 times higher compared with that by yellow eel hepatocytes. Silver eel hepatocytes secreted lipids 2.5 times higher through the lipoprotein than yellow eel hepatocytes. Main lipid was triacylglycerol in either secreted lipoprotein and composition of apolipoproteins of both secreted lipoproteins was the same. The incorporation of 3H-leucine into the lipoprotein secreted by silver eel hepatocytes was 2.4 times higher, but that of 14C-acetate was not significantly different. Protein and lipids composition of plasma lipoproteins of silver eel was significantly higher and lower compared with those of yellow eel, respectively. We suggest that the secreted lipoprotein of silver eel hepatocytes transport much more lipids to other tissues than that of yellow eel hepatocytes.
Ndiaye
D
D
United Graduate School of Agricultural Science, Kagoshima University, Japan.
Hayashi
S
S
D019342:Acetic Acid / Q000378:metabolism; D000811:Anguilla / Q000097:blood / Q000254:growth & development / Q000502:physiology*; D000818:Animals; D001797:Blood Protein Electrophoresis; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D007930:Leucine / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000097:blood / Q000302:isolation & purification; D008079:Lipoproteins, VLDL / Q000097:blood / Q000302:isolation & purification; D008099:Liver / Q000378:metabolism*
D005456:Fluorescent Dyes; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL; D007930:Leucine; D019342:Acetic Acid