Production of formyl-CoA during peroxisomal alpha-oxidation of 3-methyl-branched fatty acids.
alpha-Oxidation of 3-methyl-substituted fatty acids was studied in purified rat liver peroxisomes. The experiments revealed that formyl-CoA is formed during the alpha-oxidation process. The amount of formyl-CoA found constituted 2-5% of the amount of formate formed. Under the conditions used, no activation of exogenously added formate occurred in purified peroxisomes, whereas 95.5% of added synthetic formyl-CoA was converted to formate. These data indicate that during alpha-oxidation first formyl-CoA is formed, which is then hydrolysed to formate.
Croes
K
K
Katholieke Universiteit Leuven, Afdeling Farmacologie, Campus Gasthuisberg, Belgium.
Van Veldhoven
P P
PP
Mannaerts
G P
GP
Casteels
M
M
D000818:Animals; D008099:Liver / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000378:metabolism*; D010084:Oxidation-Reduction; D010169:Palmitic Acids / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D010169:Palmitic Acids; C034840:3-methylhexadecanoic acid