A membrane network for nutrient import in red cells infected with the malaria parasite.
The human malaria parasite Plasmodium falciparum exports an interconnected network of tubovesicular membranes (the TVM) that extends from the parasite's vacuolar membrane to the periphery of the red cell. Here it is shown that extracellular solutes such as Lucifer yellow enter the TVM and are delivered to the parasite. Blocking the assembly of the network blocked the delivery of exogenous Lucifer yellow, nucleosides, and amino acids to the parasite without inhibiting secretion of plasmodial proteins. These data suggest that the TVM is a transport network that allows nutrients efficient access to the parasite and could be used to deliver antimalarial drugs directly into the parasite.
Lauer
S A
SA
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5402, USA.
Rathod
P K
PK
Ghori
N
N
Haldar
K
K
D000241:Adenosine / Q000378:metabolism; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D020126:Brefeldin A; D003517:Cyclopentanes / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology*; D018698:Glutamic Acid / Q000378:metabolism*; D006801:Humans; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D007546:Isoquinolines / Q000378:metabolism; D009025:Morpholines / Q000494:pharmacology*; D009705:Nucleosides / Q000378:metabolism*; D015388:Organelles / Q000187:drug effects / Q000378:metabolism*; D009963:Orotic Acid / Q000378:metabolism; D010963:Plasmodium falciparum / Q000187:drug effects / Q000378:metabolism*; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000378:metabolism; D013107:Sphingolipids / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
C095823:1-phenyl-2-palmitoylamino-3-morpholino-1-propanol; D000962:Antimalarials; D003517:Cyclopentanes; D007546:Isoquinolines; D009025:Morpholines; D009705:Nucleosides; D015800:Protozoan Proteins; D013107:Sphingolipids; D020126:Brefeldin A; D018698:Glutamic Acid; D009963:Orotic Acid; C017475:lucifer yellow; D000241:Adenosine; D013936:Thymidine