title: New technique for inducing reversible obstructive jaundice in the rat.
abstract: We present a new animal model of reversible obstructive jaundice, in which two polyethylene tubes were inserted into the common bile duct, one towards the liver and the other towards the duodenum. The ends of the tubes were tunneled subcutaneously and tied shut to establish obstructive jaundice. Biliary drainage was achieved without a second laparotomy by connecting the tubes with a 1-cm segment of a 24-gauge needle. Serum concentrations of total bilirubin (TB), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), alkaline phosphatase (ALP), and albumin (Alb.) were measured before and after obstruction and decompression. TB, GOT, GPT and ALP increased and Alb. decreased 7 days after the onset of biliary obstruction. All values normalized 14 days after biliary drainage at the latest. Our model is reproducible and allows for evaluation of biliary decompression. Unlike previous models, it can be used for long-term investigation of chronic obstructive jaundice.
authors.affiliation: Department of Surgery II, Yamaguchi University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hirazawa
authors.forename: M
authors.initials: M
authors.lastname: Oka
authors.forename: Y
authors.initials: Y
authors.lastname: Ogura
authors.forename: M
authors.initials: M
authors.lastname: Miyahara
authors.forename: S
authors.initials: S
authors.lastname: Hazama
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D001835:Body Weight; D002779:Cholestasis / Q000209:etiology* / Q000503:physiopathology; D004195:Disease Models, Animal; D008099:Liver / Q000473:pathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000032:analysis; D013997:Time Factors
chemical_list: D012709:Serum Albumin; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin