Modulation of mRNA levels in the presence of thymocytes and genome mapping for a set of genes expressed in mouse thymic epithelial cells.
Modulation of gene expression in mouse thymic epithelium upon culture in the presence of thymocytes (coculture) was studied by comparison of hybridization signatures on a set of nearly 5000 mouse thymus cDNA clones. Forty-nine differentially expressed clones (usually down-regulated in coculture) were characterized by tag sequencing. Many of them corresponded to entities that had not been described previously in the mouse, and were further characterized by genome mapping. This set of genes appears to be involved in growth regulation and differentiation within the thymus.
Rocha
D
D
Genome Structure and Immune Functions Laboratory, Centre d'Immunologie INSERM-CNRS de Marseille-Luminy (CIML), Case 906, 13288 Marseille, Cedex 9, France.
Carrier
A
A
Naspetti
M
M
Victorero
G
G
Anderson
E
E
Botcherby
M
M
Guénet
J L
JL
Nguyen
C
C
Naquet
P
P
Jordan
B R
BR
D000818:Animals; D002874:Chromosome Mapping; D018920:Coculture Techniques; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005786:Gene Expression Regulation; D016678:Genome; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*; D013950:Thymus Gland / Q000166:cytology / Q000502:physiology*
D012333:RNA, Messenger