Molecular cloning of hemocyanin cDNA from Penaeus vannamei (Crustacea, Decapoda): structure, evolution and physiological aspects.
Hemocyanin is present as 2 subunits in the hemolymph of Penaeus vannamei. Isolated from a hepatopancreas cDNA library of this penaeid shrimp, the cDNA chain (2095 bp) corresponds to a full length hemocyanin messenger as determined by Northern hybridization, with a short 5' untranslated region (17 bp), an open reading frame (1989 bp counting initiation and termination codons) coding for a signal peptide (13 residues) and a mature hemocyanin (648 amino acids), and a 3' untranslated region (89 bp) followed by the polyadenylated track. It is the first time that the existence of a hydrophobic signal peptide is shown in arthropod hemocyanin. Two primary N-terminal sequences are determined and a 3-fold increase of mRNA content, measured in the hepatopancreas during the premoult stages, is reported. The low level of polymorphism shown by P. vannamei hemocyanin, along with its weak percentage identity with counterparts and its similarity with hemocyanin from Panulirus interruptus, suggests that this arthropod hemocyanin may be a primitive subunit that has evolved independently, following gene duplication.
Sellos
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Station de Biologie Marine, Museum National d'Histoire Naturelle et College de France, Concarneau. sellos@sb-roscoff.fr
Lemoine
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S
Van Wormhoudt
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A
D000595:Amino Acid Sequence; D000818:Animals; D001181:Arthropods / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006433:Hemocyanins / Q000235:genetics*; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D010179:Pancreas / Q000737:chemistry; D033561:Penaeidae / Q000235:genetics* / Q000254:growth & development; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D006433:Hemocyanins