title: Expression and identification of aberrant c-kit transcripts in human cancer cells.
abstract: We have previously cloned and sequenced a novel 3.5 kb c-kit mRNA expressed in a colon carcinoma cell line Colo201. Here we examined the expression of this truncated form of c-kit in 14 gastrointestinal cancer cells and 16 hematopoieic cancer cells by RT-PCR. Expression of the aberrant c-kit transcript was observed in various cancer cell lines. Furthermore, a new transcript which is 78 bp shorter than the transcript previously described was identified and characterized. These results indicate that two kinds of aberrant c-kit transcript produced by alternative promoter in intron 15 are expressed in human cancer cells.
authors.affiliation: First Department of Internal Medicine, Sapporo Medical University, Chuo-ku, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Takaoka
authors.forename: M
authors.initials: M
authors.lastname: Toyota
authors.forename: Y
authors.initials: Y
authors.lastname: Hinoda
authors.forename: F
authors.initials: F
authors.lastname: Itoh
authors.forename: H
authors.initials: H
authors.lastname: Mita
authors.forename: H
authors.initials: H
authors.lastname: Kakiuchi
authors.forename: M
authors.initials: M
authors.lastname: Adachi
authors.forename: K
authors.initials: K
authors.lastname: Imai
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D005770:Gastrointestinal Neoplasms / Q000235:genetics / Q000378:metabolism*; D019337:Hematologic Neoplasms / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D019009:Proto-Oncogene Proteins c-kit