Evidence for local production of acute phase response apolipoprotein serum amyloid A in Alzheimer's disease brain.
Acute phase serum amyloid A (A-apoSAA), but not constitutive apoSAA (C-apoSAA), was identified by Western blotting experiments in brain protein extracts from eight of nine patients with Alzheimer's disease (AD), one with a brain tumor and one with multiple sclerosis. A-apoSAA was not detected in six subjects with Pick's or Lewy Body disease or three other non-AD brain specimens. Apolipoprotein A-I and albumin were not found in any of the brain protein extracts. A-apoSAA mRNA was detected in AD brain by reverse transcription-polymerase chain reaction (RT-PCR). These data suggest that apoSAA is locally produced in AD brain and that investigation of the neuroinflammatory effects of this injury specific apolipoprotein is warranted.
Liang
J S
JS
Department of Biochemistry, Boston University School of Medicine, MA 02118, USA.
Sloane
J A
JA
Wells
J M
JM
Abraham
C R
CR
Fine
R E
RE
Sipe
J D
JD
D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D001053:Apolipoproteins / Q000096:biosynthesis* / Q000378:metabolism*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D013997:Time Factors
D001053:Apolipoproteins; D000685:Serum Amyloid A Protein