title: Abnormal structure of the Y chromosome detected in bovine gonadal hypoplasia (XY female) by FISH.
abstract: The structure of the Y chromosome was investigated by FISH and G banding in 3 cases of bovine XY females shown by PCR to lack the SRY gene. Although 2 different repeat-DNA sequences (BC1.2 and btDYZ-1) hybridized to the short arm of the Y chromosome in a normal bull, they hybridized to both arms of the Y chromosome in the XY females. In the Y chromosome from XY females, only the centromeric region was darkly stained by G banding, in comparison with dark staining on the long arm of a normal Y chromosome. From the results obtained, it seemed that the Y chromosome from the XY females was structurally abnormal, namely an isochromosome with an identical short arm. Our results also confirm that SRY is located on the long arm of the Y chromosome in bovine.
authors.affiliation: National Institute of Animal Industry, Tsukuba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kawakura
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
authors.forename: R K
authors.initials: RK
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Kondoh
authors.forename: T I
authors.initials: TI
authors.lastname: Hirata
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneda
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D006061:Gonadal Dysgenesis, 46,XY / Q000235:genetics / Q000662:veterinary*; D017404:In Situ Hybridization, Fluorescence; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014998:Y Chromosome
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors