title: Lack of effect of antineutrophil cytoplasmic antibodies associated with ulcerative colitis on superoxide anion production from neutrophils.
abstract: BACKGROUND
  Antineutrophil cytoplasmic antibodies (ANCAs) from patients with vasculitidis can induce neutrophils to release oxygen radicals in vitro. ANCAs with a perinuclear pattern of immunofluorescence are found in most patients with ulcerative colitis, but several findings are against ANCAs having a pathogenetic role in this disease.
  
  
  OBJECTIVE
  To evaluate the influence of ANCAs associated with ulcerative colitis on the respiratory burst activity of neutrophils.
  
  
  METHODS
  Serum samples were obtained from 14 patients with ulcerative colitis, seven of whom showed positivity for p-ANCAs, three patients with vasculitidis, two with positivity for p-ANCAs, and one for c-ANCAs, and seven healthy volunteers.
  
  
  METHODS
  A positive ANCA serology was determined with a standard indirect immunofluorescence assay. Purified immunoglobulins (IgGs) were prepared from serum samples by DEAE-Affigel blue chromatography. Human neutrophils were prepared by dextran-Ficoll-Hypaque separation. Superoxide anion (O2-.) generation was measured by following the superoxide dismutase inhibitable reduction of ferricytochrome.
  
  
  RESULTS
  There were no significant differences among samples from ulcerative colitis IgG p-ANCA positive, ulcerative colitis IgG p-ANCA negative patients, and controls on O2-. production, whereas ANCA positive IgG from vasculitidis significantly enhanced O2-. release (p < 0.001).
  
  
  CONCLUSIONS
  p-ANCAs associated with ulcerative colitis have no effect on the respiratory burst activity of normal human neutrophils in vitro. These results reinforce the hypotheses that ANCAs are unlikely to contribute to the pathogenesis of ulcerative colitis.
authors.affiliation: Istituto di Clinica Medica e Gastroenterologia, Universitá di Bologna, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Gionchetti
authors.forename: M
authors.initials: M
authors.lastname: Vecchi
authors.forename: F
authors.initials: F
authors.lastname: Rizzello
authors.forename: M
authors.initials: M
authors.lastname: Ferretti
authors.forename: C
authors.initials: C
authors.lastname: Calabresi
authors.forename: A
authors.initials: A
authors.lastname: Venturi
authors.forename: M B
authors.initials: MB
authors.lastname: Bianchi
authors.forename: C
authors.initials: C
authors.lastname: Brignola
authors.forename: R A
authors.initials: RA
authors.lastname: Sinico
authors.forename: R
authors.initials: R
authors.lastname: De Franchis
authors.forename: M
authors.initials: M
authors.lastname: Miglioli
authors.forename: M
authors.initials: M
authors.lastname: Campieri
mesh_terms: D019268:Antibodies, Antineutrophil Cytoplasmic / Q000494:pharmacology*; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism*; D016897:Respiratory Burst / Q000187:drug effects*; D013481:Superoxides / Q000378:metabolism*; D014657:Vasculitis / Q000378:metabolism
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D007074:Immunoglobulin G; D013481:Superoxides