title: Purification and some properties of endo-1,4-beta-D-xylanase from a fresh-water mollusc, Pomacea insularus (de Ordigny).
abstract: Endo-1,4-beta-D-xylanase (EC 3.2.1.8) was purified from viscera of a fresh-water mollusc, Pomacea insularus (de Ordigny). The purified enzyme, with a molecular weight of 47,000, gave a single protein band in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The amino-terminal sequence was Ala-Ala-Gly-Ala-Gly-Val-Thr-Ser-Glu-Lys-Asp-Arg-Leu-Arg-Arg-Ser-Asp-Lys -Thr-Val-His-Val-Asn-. The enzyme was stable from pH about 4.5 to 9.5 and had its maximum activity at pH about 5.5. The purified enzyme produced X2, X3, X4, and larger xylooligosaccharides from birchwood xylan. The enzyme activity was greatly inhibited by Ag+, Hg2+, Cu2+, N-bromosuccinimide, and p-chloromercuribenzoic acid. On the other hand, the enzyme activity was greatly elevated by the addition of chloride ion.
authors.affiliation: Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Yamaura
authors.forename: T
authors.initials: T
authors.lastname: Koga
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Kato
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D017610:Calcium Compounds / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases; D004789:Enzyme Activation; D004795:Enzyme Stability; D006868:Hydrolysis; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008974:Mollusca / Q000201:enzymology*; D009566:Nitrates / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D017610:Calcium Compounds; D009566:Nitrates; D014990:Xylans; D012965:Sodium Chloride; C031618:sodium nitrate; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases; D002122:Calcium Chloride; C059948:calcium nitrate