[Site-specific endonuclease BspR7I from thermophilic strain of Bacillus sp. R7].
A site-specific endonuclease which recognizes the sequence 5'-CCTNAGG-3' was purified to homogeneity from the thermophilic strain Bacillus sp. R7. The endonuclease (BspR7I) is monomeric protein with an apparent molecular weight of 37 kD. The enzyme is active over a wide range of NaCl concentrations, pH, and temperatures. BspR7I cleaves DNA substrates according to the scheme: 5'-CC decreases TNAGG-3' 3'-GGANT increases CC-5', hence the endonuclease represents an isoschizomer of Bsu361.
Vasil'eva
L Iu
LIu
Zheleznaia
L A
LA
Matvienko
N I
NI
D001407:Bacillus / Q000201:enzymology*; D002850:Chromatography, Gel; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification*; D004795:Enzyme Stability; D008970:Molecular Weight; D013379:Substrate Specificity
D015252:Deoxyribonucleases, Type II Site-Specific; C062194:endodeoxyribonuclease SauI
Saĭt-spetsificheskaia éndonukleaza BspR7I iz termofil'nogo shtamma Bacillus sp. R7.