A general method for DNA polymorphism identification in genetic assessment and molecular diagnosis.
A general methodology for the analysis of human diseases at the nucleic acid level has been described. This method consists of a semiautomated, nontoxic analytic procedure, staining DNA bands with silver nitrate instead of ethidium bromide after DNA amplification by PCR. This technique provides a sensitive assay with enhanced accuracy and offers the possibility of carrying out the molecular analysis of several polymorphisms in a single day simplifying molecular genetics with diagnostic purposes.
Beyer
K
K
EuroEspes Biomedical Research Center, Department of Clinical and Molecular Genetics, Molecular Neuropharmacology and Neurogeriatrics, La Coruña, Spain.
Lao
J I
JI
Alvarez
X A
XA
Cacabelos
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R
D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D030342:Genetic Diseases, Inborn / Q000175:diagnosis*; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012835:Silver Nitrate
D004247:DNA; D012835:Silver Nitrate