[Bacterial translocation in acute pancreatitis. Experimental study in rats].
Ninety Wistar rats were studied to confirm if the intestinal tract is a source of infection in acute pancreatitis. Bacterial translocation to the mesenteric lymph nodes, pancreas, peritoneal cavity, and blood was determined at 6 h, 24 h, 48 h and 96 hours after induction of non lethal acute pancreatitis. Bacterial growing was present in 60% (6 h), 90% (24 h), 70% (48 h) and 40% (96 h) (p < 0.05) of the mesenteric lymph nodes. Live bacteria were recovered from 67% (6 h), 90% (24 h), 50% (48 h) and 40% (96 h) (p < 0.05) of the pancreas. Gram-positive bacteria translocate more frequently than Gram-negative bacteria in the early period (6 h). Lately (24-96 h), Gram-negative bacteria were much more frequently isolated (p < 0.05). The most common organism isolated was Escherichia coli. No overgrowth of cecal Gram-negative bacteria was found. It is concluded that bacterial translocation in acute pancreatitis is an early phenomena, initially caused by Gram-positive bacteria and lately by Gram-negative bacteria, and associated with the severity of pancreatic lesions.
de Souza
L
L
Laboratório de Investigao Médica da Disciplina de Cirurgia do Aparelho Digestivo (LIM 35), Faculdade de Medicina, Universidade de São Paulo.
Sampietre
S N
SN
Figueiredo
S
S
Yria
Y
Y
Machado
M C
MC
Pinotti
H W
HW
D000208:Acute Disease; D000818:Animals; D018988:Bacterial Translocation; D002756:Cholagogues and Choleretics / Q000009:adverse effects; D006090:Gram-Negative Bacteria / Q000302:isolation & purification / Q000502:physiology*; D006094:Gram-Positive Bacteria / Q000302:isolation & purification / Q000502:physiology*; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D008643:Mesentery / Q000382:microbiology; D010179:Pancreas / Q000382:microbiology; D010195:Pancreatitis / Q000139:chemically induced / Q000382:microbiology*; D051381:Rats; D017208:Rats, Wistar; D013656:Taurocholic Acid / Q000009:adverse effects
D002756:Cholagogues and Choleretics; D013656:Taurocholic Acid
Translocação bacteriana na pancreatite aguda. Estudo experimental em ratos.