Bacterial surface display: trends and progress.
Heterologous surface display on Gram-negative bacterial was first described a decade ago and is now an active research area. More recently, strategies for surface display on Gram-positive bacterial have also been devised and these carry some inherent advantages. Bacterial surface display has found a range of applications in the expression of various antigenic determinants, heterologous enzymes, single-chain antibodies, polyhistidyl tags and even entire peptide libraries. This article explains the basis of bacterial surface display and discusses current uses and possible future trends of this emerging technology.
Ståhl
S
S
Department of Biochemistry and Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden. stefans@biochem.kth.se
Uhlén
M
M
D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000302:isolation & purification; D001709:Biotechnology; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D000939:Epitopes / Q000235:genetics / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000378:metabolism; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism*; D014614:Vaccines, Synthetic / Q000302:isolation & purification
D001428:Bacterial Vaccines; D004798:Enzymes; D000939:Epitopes; D007128:Immunoglobulin Fragments; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic