Characterization of the interactions of alpha-catenin with alpha-actinin and beta-catenin/plakoglobin.
Cadherins are calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. To function in cell-cell adhesion, the transmembrane cadherin molecule must be associated with the cytoskeleton via cytoplasmic proteins known as catenins. Three catenins, alpha-catenin, beta-catenin and gamma-catenin (also known as plakoglobin), have been identified. beta-catenin or plakoglobin is associated directly with the cadherin; alpha-catenin binds to beta-catenin/plakoglobin and serves to link the cadherin/catenin complex to the actin cytoskeleton. The domains on the cadherin and betacatenin/plakoglobin that are responsible for protein-protein interactions have been mapped. However, little is known about the molecular interactions between alpha-catenin and beta-catenin/plakoglobin or about the interactions between alpha-catenin and the cytoskeleton. In this study we have used the yeast two-hybrid system to map the domains on alpha-catenin that allow it to associate with beta-catenin/plakoglobin and with alpha-actinin. We also identify a region on alpha-actinin that is responsible for its interaction with alpha-catenin. The yeast two-hybrid data were confirmed with biochemical studies.
Nieset
J E
JE
Department of Biology, University of Toledo, OH 43606, USA.
Redfield
A R
AR
Jin
F
F
Knudsen
K A
KA
Johnson
K R
KR
Wheelock
M J
MJ
D000185:Actinin / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism*; D051180:Desmoplakins; D006801:Humans; D010449:Peptide Mapping; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D014162:Transfection; D051178:alpha Catenin; D051185:gamma Catenin
C495273:CTNNA1 protein, human; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; C495292:JUP protein, human; D011993:Recombinant Fusion Proteins; D051178:alpha Catenin; D051185:gamma Catenin; D000185:Actinin