title: Elastin degradation in the superficial temporal arteries of patients with intracranial aneurysms reflects changes in plasma elastase.
abstract: OBJECTIVE
  alpha 1-Antitrypsin (AAT) and alpha 2-macroglobulin (AMG) are elastase inhibitors that bind the enzyme and reduce measured levels of free elastase. It was recently demonstrated that some patients with intracranial aneurysms have significantly elevated plasma elastase (PE) levels. Although this elevation is unrelated to plasma AAT, it is unknown whether abnormal AAT phenotypes or reduced AMG levels play a role. Moreover, the pathological significance of this elevation is not understood.
  
  
  METHODS
  Plasma from 24 patients with aneurysms (ruptured, n = 15; unruptured, n = 9) and 10 age-matched patients who comprised a control group was analyzed for PE and AMG levels by enzyme-linked immunosorbent assay and for AAT phenotype by isoelectric focusing. Sections of superficial temporal temporal artery obtained from these patients at the time of surgery were examined for evidence of elastin degradation by using a van Gieson stain, with scoring on a nine-point quantitative scale.
  
  
  RESULTS
  Patients with aneurysms showed significantly elevated PE levels (119 +/- 28 versus 17 +/- 7 micrograms/ml, P < 0.05), but AMG levels were not decreased. AAT phenotypic abnormalities were observed in 10% (2 of 20) of the patients with aneurysms, but this was not different from the expected population incidence (7%). Elastin degradation scores were significantly higher in patients with aneurysms than in patients control group (4.26 +/- 0.54 versus 1.21 +/- 0.43, P < 0.05). In addition, patients with higher elastase levels (> 80 micrograms/ml) demonstrated 55% higher degradation scores than did those with lower elastase levels (< 80 micrograms/ml).
  
  
  CONCLUSIONS
  These data suggest that high PE levels may play a role in systemic arterial elastin degradation seen in patients with intracranial aneurysms. These data also support the contention that elevated elastase levels are not the result of decreased protease inhibitor levels. Although PE levels were significantly higher for the entire group of patients with aneurysms, this assay has relatively low sensitivity for predicting the presence of unruptured aneurysms. Additional study is necessary to determine whether serum elastase levels greater than 80 micrograms/ml, in the setting of other risk factors, are useful in identifying asymptomatic patients for additional screening.
authors.affiliation: Department of Neurological Surgery, Columbia University, College of Physicians and Surgeons, New York, New York, USA.
authors.forename: E S
authors.initials: ES
authors.lastname: Connolly
authors.forename: A J
authors.initials: AJ
authors.lastname: Fiore
authors.forename: C J
authors.initials: CJ
authors.lastname: Winfree
authors.forename: C J
authors.initials: CJ
authors.lastname: Prestigiacoma
authors.forename: J E
authors.initials: JE
authors.lastname: Goldman
authors.forename: R A
authors.initials: RA
authors.lastname: Solomon
mesh_terms: D000328:Adult; D000368:Aged; D017542:Aneurysm, Ruptured / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology / Q000601:surgery; D003399:Craniotomy; D004547:Elastic Tissue / Q000201:enzymology / Q000473:pathology; D004549:Elastin / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D002532:Intracranial Aneurysm / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000097:blood*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012016:Reference Values; D013345:Subarachnoid Hemorrhage / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology / Q000601:surgery; D013699:Temporal Arteries / Q000201:enzymology / Q000473:pathology*; D000515:alpha 1-Antitrypsin / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D004549:Elastin; D010196:Pancreatic Elastase