title: Protein tyrosine phosphorylation influences adhesive junction assembly and follicular organization of cultured thyroid epithelial cells.
abstract: The follicular histoarchitecture of the thyroid forms the anatomical basis for thyroid physiology and is commonly disturbed in diseases of the thyroid. We have used cultured porcine thyroid cells to study thyroid epithelial morphogenesis and its regulation. When cultured in the presence of TSH, freshly isolated thyroid cells reorganize to form follicles within three-dimensional cell aggregates. However, when established follicles are washed into TSH-free medium, thyroid cells spread and migrate to convert follicles into confluent epithelioid monolayers, activating morphogenetic mechanisms, such as cell locomotility, that may be relevant to thyroid inflammation and tumor invasiveness. The phenomenon of follicle to monolayer conversion, therefore, provides an opportunity to identify morphogenetic mechanisms that 1) must be tonically inhibited to maintain follicular organization and 2) may contribute to pathogenetic disturbances of follicular architecture when functioning aberrantly. In this study we found that follicle to monolayer conversion is associated with an increase in cellular phosphotyrosine. This was particularly evident at nascent focal adhesions (cell-substrate adhesive junctions) and later at cell-cell junctions. Focal adhesion assembly was accompanied by reorganization of the actin cytoskeleton, with the appearance of prominent stress fibers. Genistein, a potent inhibitor of protein tyrosine kinases, inhibited the accumulation of phosphotyrosine, focal adhesion assembly, and follicle to monolayer conversion. We conclude that tyrosine phosphorylation exerts an important influence on thyroid epithelial organization in culture, at least partly mediated through regulation of focal adhesion assembly.
authors.affiliation: Department of Physiology and Pharmacology, University of Queensland, St. Lucia, Brisbane, Australia. yap@plpk.uq.oz.au
authors.forename: A S
authors.initials: AS
authors.lastname: Yap
authors.forename: B R
authors.initials: BR
authors.lastname: Stevenson
authors.forename: V
authors.initials: V
authors.lastname: Cooper
authors.forename: S W
authors.initials: SW
authors.lastname: Manley
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D019833:Genistein; D007365:Intercellular Junctions / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D007529:Isoflavones / Q000494:pharmacology*; D009024:Morphogenesis; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013552:Swine; D013972:Thyrotropin / Q000494:pharmacology*; D019108:Tight Junctions / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D016596:Vinculin / Q000032:analysis / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D007529:Isoflavones; D016596:Vinculin; D019000:Phosphotyrosine; D013972:Thyrotropin; D019833:Genistein; D011505:Protein-Tyrosine Kinases