Rodent malaria parasites: molecular karyotypes characterize species, subspecies and lines.
The molecular karyotypes of the African murine malaria parasites P. berghei (3 strains, 2 lines) P. yoeli (2 strains) P. chabaudi (3 strains, 1 line) and P. vinckei (4 strains) have been studied using orthogonal field alternation gel electrophoresis (OFAGE). The genome of each species was resolved into 9 to 11 distinct chromosomal DNA banas molecules of varying intensities which seem to represent 14 chromosomes ranging in size from 600 kb to 3500 kb. The position of certain chromosomes allowed the identification of a unique karyotype for each of the strains and lines under study. P. yoelii appears by criteria of chromosome size, chromosome numbers and localisation of DNA probes to differ considerably from the other three rodent malaria species. The chromosomal location of 5 DNA probes allowed the identification of corresponding chromosomes in rodent malaria parasites and the differentiation between species and strains. Assignment of the "PMMSA" gene of P. c. chabaudi IP-PC 1 enables the distinction of the four rodent malaria species. The molecular karyotype combined to chromosomal assignment of DNA probes provides a useful tool for a more precise characterization by a genetic definition of malaria parasites.
Ahamada Said
Laboratory for Protozoology, Institute of Tropical Medicine, Antwerpen, Belgique.
Wery
M
M
Hamers
R
R
D000818:Animals; D015139:Blotting, Southern / Q000662:veterinary; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005819:Genetic Markers; D007621:Karyotyping / Q000662:veterinary; D008288:Malaria / Q000469:parasitology / Q000662:veterinary*; D051379:Mice; D010961:Plasmodium / Q000145:classification* / Q000235:genetics; D010962:Plasmodium berghei / Q000145:classification / Q000235:genetics; D016788:Plasmodium chabaudi / Q000145:classification / Q000235:genetics; D010967:Plasmodium yoelii / Q000145:classification / Q000235:genetics; D012376:Rodent Diseases / Q000469:parasitology*
D015342:DNA Probes; D016054:DNA, Protozoan; D005819:Genetic Markers