HIP-I: a huntingtin interacting protein isolated by the yeast two-hybrid system.
We report the discovery of the huntingtin interacting protein I (HIP-I) which binds specifically to the N-terminus of human huntingtin, both in the two-hybrid screen and in in vitro binding experiments. For the interaction in vivo, a protein region downstream of the polyglutamine stretch in huntingtin is essential. The HIP1 cDNA isolated by the two-hybrid screen encodes a 55 kDa fragment of a novel protein. Using an affinity-purified polyclonal antibody raised against recombinant HIP-I, a protein of 116 kDa was detected in brain extracts by Western blot analysis. The predicted amino acid sequence of the HIP-I fragment exhibits significant similarity to cytoskeleton proteins, suggesting that HIP-I and huntingtin play a functional role in the cell filament networks. The HIP1 gene is ubiquitously expressed in different brain regions at low level. HIP-I is enriched in human brain but can also be detected in other human tissues as well as in mouse brain. HIP-I and huntingtin behave almost identically during subcellular fractionation and both proteins are enriched in the membrane containing fractions.
Wanker
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EE
Max Planck Institut für Molekulare Genetik, Berlin (Dahlem), Germany.
Rovira
C
C
Scherzinger
E
E
Hasenbank
R
R
Wälter
S
S
Tait
D
D
Colicelli
J
J
Lehrach
H
H
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D001923:Brain Chemistry; D003001:Cloning, Molecular; D003599:Cytoskeleton / Q000737:chemistry; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006801:Humans; D000071058:Huntingtin Protein; D006816:Huntington Disease / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017421:Sequence Analysis
D004268:DNA-Binding Proteins; C096896:HAP1 protein, human; C073368:HIP1 protein, human; C086055:HTT protein, human; C490758:Hap1 protein, mouse; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; C097188:polyglutamine