Ultraviolet rays induced expression of lectins on the surface of a squamous carcinoma keratinocyte cell line.
Human keratinocytic cells from squamous carcinoma (SCL-1) present, under resting conditions, relatively low amounts of endogenous lectins (sugar-binding proteins). Upon uv irradiation, they express on their cell surface large amounts of endogenous lectin molecules able to bind neoglycoproteins bearing either alpha-L-rhamnosyl or alpha-D-glucosyl residues. A similar binding specificity was found with normal human keratinocytes under the same culture conditions. At sunlike doses, uv.A (365 nm) was more efficient than uv.B (312 nm) in the expression of such receptors on the surface of SCL-1 cells. The increased presentation of lectins by SCL-1 cells was transient and reached a maximum 4 h after irradiation. Such a specific modulation of receptor expression upon uv irradiation might be biologically significant, considering the numerous intercellular recognition phenomena in skin biology. alpha-L-Rhamnose-specific receptor on SCL-1 could not be distinguished from alpha-D-glucose-specific receptor on the basis of neoglycoproteins binding, uptake, and related inhibitions. Lectin expression was mainly detected on the cell surface, and its overexpression due to uv rays required a de novo protein synthesis process.
Condaminet
B
B
Centre de Biophysique Moléculaire, CNRS et Université d'Orléans, France.
Redziniak
G
G
Monsigny
M
M
Kieda
C
C
D002236:Carbohydrate Conformation; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D005947:Glucose / Q000032:analysis; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000528:radiation effects*; D037102:Lectins / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012210:Rhamnose / Q000032:analysis; D012503:Saponins / Q000494:pharmacology; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014466:Ultraviolet Rays
D006023:Glycoproteins; D037102:Lectins; D009363:Neoplasm Proteins; D012503:Saponins; D005947:Glucose; D012210:Rhamnose