Detection of mutant p53 in clam leukemia cells.
Leukemia in the soft-shell clam, Mya arenaria, is characterized by tumor cells which are detected initially in the hemolymph. This disease is much more common in clams inhabiting polluted waters, suggesting an environmental component to its pathogenesis. In this study, leukemia cells were identified using a murine monoclonal antibody, 1E10, which recognizes a leukemia-specific protein expressed by tumor cells. Mutant p53 protein was detected using a murine monoclonal antibody (PAb 240) which reacts with mutant p53. Using immunofluorescence, the reactivity of clam cells to the 1E10 antibody was evaluated along with mutant p53 protein reactivity. Reverse transcriptase-polymerase chain reactions followed by sequence analyses were utilized to examine clams with hemocytes reacting with the p53 antibody for possible p53 gene mutations. Mutant p53 protein was expressed by tumor cells from five animals with advanced disease (in which greater than 90% of cells reacted with 1E10). A C-->G transversion was detected at the end of exon 6 from two of the five animals that reacted with both the mutant p53 antibody and 1E10. This substitution changes the amino acid of this codon from proline to alanine. Overall, our results suggest that environmentally induced alterations in p53 can contribute to the pathogenesis of leukemia in soft-shell clams inhabiting polluted water and/or sediment.
Barker
C M
CM
Department of Comparative Medicine, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA.
Calvert
R J
RJ
Walker
C W
CW
Reinisch
C L
CL
D000818:Animals; D049872:Bivalvia / Q000235:genetics*; D003043:Cocarcinogenesis; D003062:Codon / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect; D016158:Genes, p53; D006434:Hemocytes / Q000737:chemistry / Q000473:pathology*; D006458:Hemolymph / Q000166:cytology*; D007938:Leukemia / Q000139:chemically induced / Q000235:genetics / Q000662:veterinary*; D009363:Neoplasm Proteins / Q000097:blood; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000097:blood; D014873:Water Pollutants / Q000009:adverse effects; D015262:Xenobiotics / Q000009:adverse effects
D003062:Codon; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53; D014873:Water Pollutants; D015262:Xenobiotics