Immunohistochemical study of myelin-specific proteins in pt rabbits.
The cellular/regional expression of myelin-specific proteins: PLP, MBP, CNP-ase, MAG and MOG was investigated in the brains of 14 and 42 days old control and pt-mutant rabbits. The results showed severe reduction in expression of PLP protein, the known molecular target of pt mutation. The minor differences in immunostaining of the other studied myelin-connected proteins between normal and mutant rabbits confirmed once more the deficient and delayed myelination in pt brain. No signs of the increased retention of neither PLP nor any other protein in pt oligodendrocytes were evidenced in this study.
Domańska-Janik
K
K
Department of Neurochemistry, Polish Academy of Sciences, Warszawa.
Sypecka
J
J
Taraszewska
A
A
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000032:analysis / Q000096:biosynthesis; D000367:Age Factors; D000818:Animals; D002490:Central Nervous System / Q000737:chemistry*; D007150:Immunohistochemistry; D004676:Myelin Basic Protein / Q000032:analysis / Q000096:biosynthesis; D009185:Myelin Proteins / Q000032:analysis* / Q000096:biosynthesis; D018991:Myelin Proteolipid Protein / Q000737:chemistry* / Q000172:deficiency / Q000235:genetics; D009186:Myelin Sheath / Q000737:chemistry* / Q000473:pathology; D018992:Myelin-Associated Glycoprotein / Q000032:analysis / Q000096:biosynthesis; D063308:Myelin-Oligodendrocyte Glycoprotein; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011817:Rabbits; D014202:Tremor / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D009419:Nerve Tissue Proteins; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases