title: Primary structure of the smallest (6.4-kDa) subunit of human and bovine ubiquinol-cytochrome c reductase deduced from cDNA sequences.
abstract: Amino acid sequences of the smallest subunit of human and bovine ubiquinol-cytochrome c reductase were deduced from nucleotide sequence of recombinant cDNA clones isolated by screening the corresponding cDNA libraries. Both proteins were composed of 56 amino acids. They were 84% homologous to each other in the coding nucleotide sequences and 88% homologous in the amino acid sequences. Southern blot analysis with human DNA suggested the presence of a single gene coding for the protein. Northern blot of human mRNAs from different tissues confirmed the existence of a single species of transcript among the tissues but the human gene is highly expressed in bioenergetically active tissues like heart and skeletal muscle.
authors.affiliation: Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
authors.forename: M M
authors.initials: MM
authors.lastname: Islam
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Yoneda
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002417:Cattle; D015342:DNA Probes / Q000302:isolation & purification; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D014450:Electron Transport Complex III / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics; D009206:Myocardium / Q000201:enzymology; D017433:Protein Structure, Secondary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013329:Structure-Activity Relationship
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D009124:Muscle Proteins; D014450:Electron Transport Complex III