title: Microprobe X-ray absorption spectroscopic determination of the oxidation state of intracellular chromium following exposure of V79 Chinese hamster lung cells to genotoxic chromium complexes.
abstract: The oxidation state of intracellular chromium has been determined directly in mammalian lung cells exposed to mutagenic and carcinogenic chromium compounds. Microprobe X-ray absorption spectroscopy (XAS) experiments on single V79 Chinese hamster lung cells showed that Cr(VI) and Cr(V) complexes were reduced completely (>90%) to Cr(III) within 4 h of exposure of the cells. This result provides direct evidence for the hypothesis that these genotoxic oxidants react rapidly with intracellular reductants.
authors.affiliation: School of Chemistry, University of Sydney, NSW, Australia.
authors.forename: C T
authors.initials: CT
authors.lastname: Dillon
authors.forename: P A
authors.initials: PA
authors.lastname: Lay
authors.forename: M
authors.initials: M
authors.lastname: Cholewa
authors.forename: G J
authors.initials: GJ
authors.lastname: Legge
authors.forename: A M
authors.initials: AM
authors.lastname: Bonin
authors.forename: T J
authors.initials: TJ
authors.lastname: Collins
authors.forename: K L
authors.initials: KL
authors.lastname: Kostka
authors.forename: G
authors.initials: G
authors.lastname: Shea-McCarthy
mesh_terms: D000818:Animals; D002460:Cell Line; D002857:Chromium / Q000378:metabolism* / Q000633:toxicity; D017608:Chromium Compounds / Q000633:toxicity*; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000187:drug effects*; D007424:Intracellular Fluid / Q000187:drug effects* / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008839:Microelectrodes; D009152:Mutagenicity Tests; D010084:Oxidation-Reduction; D013054:Spectrophotometry, Atomic; D014961:X-Ray Diffraction
chemical_list: D017608:Chromium Compounds; D002857:Chromium; D004247:DNA