title: Prevalence of the virulence-associated gene of Rhodococcus equi in isolates from infected foals.
abstract: The prevalence of the plasmid-encoded virulence-associated gene (vapA) of Rhodococcus equi, as determined by PCR, was found to be 98% in isolates from 154 foals with pneumonia, confirming the strong association of vapA with virulence. The vapA genes from 60 representative isolates were compared by digestion with the restriction endonuclease HinfI, and no evidence of sequence variation was detected.
authors.affiliation: Veterinary Preclinical Centre, Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: R E
authors.initials: RE
authors.lastname: Haites
authors.forename: G
authors.initials: G
authors.lastname: Muscatello
authors.forename: A P
authors.initials: AP
authors.lastname: Begg
authors.forename: G F
authors.initials: GF
authors.lastname: Browning
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology / Q000662:veterinary; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses; D008562:Membrane Glycoproteins / Q000235:genetics*; D008970:Molecular Weight; D010957:Plasmids / Q000032:analysis / Q000737:chemistry; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods; D016929:Rhodococcus equi / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors
chemical_list: D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D037521:Virulence Factors