[Integral kinetics of multisubstrate enzyme reactions. Criteria of kinetic behavior and characteristic coordinates for solution of direct and reverse problems].
General schemes of unbranched multisubstrate enzyme reactions associated with enzyme inactivation during catalysis are analyzed. Equations of integral kinetics at constant substrate concentrations and at depletion in one of the substrate are presented. Experimental dose-response curve characterized by additivity of some enzyme intermediates (absorption spectra, fluorescence, EPR, etc.) are theoretically analyzed. Also rapid equilibrium at certain stages of enzyme reaction is considered. The interrelationship of enzyme intermediates is a criterion of the kinetic behavior of enzyme reaction mechanism. New coordinates are suggested for the analysis of the integral kinetics of self-inactivating enzymes. Results of the analysis are used for interpretation of the data on arachidonic cascade enzymes.
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D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D007700:Kinetics; D008297:Male; D008962:Models, Theoretical; D011451:Prostaglandin-Endoperoxide Synthases / Q000302:isolation & purification / Q000378:metabolism*; D012756:Sheep; D013379:Substrate Specificity
D016718:Arachidonic Acid; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone
Integral'naia kinetika mnogosubstratnykh fermentativnykh reaktsiĭ. Kriterii kineticheskogo povedeniia i kharakteristicheskie koordinaty dlia resheniia priamoĭ i obratnoĭ zadach.