2,4-dichlorophenoxyacetic acid through lactation induces astrogliosis in rat brain.
Comparison of astroglial immunoreactivity in mesencephalon, cerebellum, and hippocampus of 25-d-old rat pups exposed to 2,4-dichlorophenoxyacetic acid (2,4-D) through the mother's milk was made using a quantitative immunohistochemical analysis. A glial reaction was detected at the level of serotonergic nuclei and extreme astrogliosis in the hippocampus and cerebellum. A quantitative analysis of reactive astrocytes was performed by using GFAP and S-100 protein as specific markers. The study showed a significant increase in their number, size, number of processes, and density of immunostaining in 2,4-D-exposed animals. Exposure to 2,4-dichlorophenoxyacetic acid on the first days of life modifies the astroglial cytoarchitecture in parallel to previously described neuronal changes.
Brusco
A
A
Institute for Cell Biology and Neuroscience Prof. Eduardo De Robertis, School of Medicine, University of Buenos Aires, Argentina.
Saavedra
J P
JP
García
G
G
Tagliaferro
P
P
Evangelista de Duffard
A M
AM
Duffard
R
R
D015084:2,4-Dichlorophenoxyacetic Acid / Q000009:adverse effects*; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism / Q000473:pathology*; D001923:Brain Chemistry; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism; D005911:Gliosis / Q000139:chemically induced* / Q000473:pathology; D006540:Herbicides / Q000009:adverse effects*; D007150:Immunohistochemistry; D007774:Lactation / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling
D005904:Glial Fibrillary Acidic Protein; D006540:Herbicides; D015084:2,4-Dichlorophenoxyacetic Acid