title: [A promoter responsible for over-expression of cholera toxin B subunit in cholera toxin A subunit structure gene].
abstract: A promoter sequence, which promotes the transcription of cholera toxin B subunit gene, was found in cholera toxin A subunit structure gene. The transcription starts at the adenine Located at +833, that is 456bp upstream to the A of the initiation codon ATG of cholera toxin B gene. Under the control of the promoter, cholera toxin B subunit was over-expressed as high as 200 mg/L at an optimized culture condition. The chloramphenicol acetyl transferase gene and beta-galactosidase could also be efficiently expressed under the direction of the promoter. This promoter may be responsible for the 6 fold and 7 fold higher expression level of cholera toxin B subunit than cholera toxin A subunit in V. cholerae and Escheria coli respectively. The over-expression of CTB may be useful in preparing vaccine against cholera and facilitating the construction of peptide-bearing immunogenic hybrid proteins.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing.
authors.forename: C
authors.initials: C
authors.lastname: Cao
authors.forename: C
authors.initials: C
authors.lastname: Shi
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Ma
mesh_terms: D001483:Base Sequence; D002772:Cholera Toxin / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
chemical_list: D002772:Cholera Toxin