Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra.
A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.
Hackbarth
C J
CJ
Department of Medicine, University of California, San Francisco 94143, USA. corinne@itsa.ucsf.edu
Unsal
I
I
Chambers
H F
HF
D000595:Amino Acid Sequence; D002440:Cefoxitin / Q000494:pharmacology; D002513:Cephamycins / Q000494:pharmacology; D003001:Cloning, Molecular; D003360:Cosmids; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics; D017421:Sequence Analysis; D001618:beta-Lactamases / Q000145:classification / Q000187:drug effects / Q000235:genetics*
D002513:Cephamycins; D002440:Cefoxitin; D001618:beta-Lactamases