A novel G protein-coupled receptor with homology to neuropeptide and chemoattractant receptors expressed during bone development.
A novel G protein-coupled receptor was isolated from a cDNA derived from the cell line NH15-CA2 and a cDNA library from adult mouse brain using a PCR cloning strategy. The amino acid sequence of the candidate receptor DEZ showed homology to neuropeptide and chemoattractant receptors. Highest overall homology was found with the orphan receptor GPR-1 (65%), the angiotensin II receptor (62%), and the C5a anaphylatoxin receptor (60%). Northern blot analysis of dez revealed a predominant 2.6 kb mRNA species in NH15-CA2 cells. In situ hybridization experiments showed that dez is differentially regulated during development, with a prominent expression in developing osseous and cartilaginous tissue. It was also detectable in the adult parathyroid glands, hinting at a possible function in bone metabolism.
Methner
A
A
Center for Molecular Neurobiology, University of Hamburg, Germany.
Hermey
G
G
Schinke
B
B
Hermans-Borgmeyer
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001846:Bone Development; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism; D001921:Brain / Q000196:embryology / Q000378:metabolism; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000737:chemistry*; D018013:Receptors, Neuropeptide / Q000096:biosynthesis / Q000737:chemistry*; D018000:Receptors, Peptide / Q000096:biosynthesis / Q000737:chemistry*
D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018013:Receptors, Neuropeptide; D018000:Receptors, Peptide; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019204:GTP-Binding Proteins