Isolation from coastal sea water and characterization of bacterial strains involved in non-ionic surfactant degradation.
A bacterial community degrading branched alkylphenol ethoxylate (APE) was selected from coastal sea water intermittently polluted by urban sewage. This community degraded more than 99% of a standard surfactant, TRITON X 100, but I.R. analysis of the remaining compound showed the accumulation of APE2 (alkylphenol with a two units length ethoxylated chain) which seemed very recalcitrant to further biodegradation. Twenty-five strains were isolated from this community, essentially Gram negative and were related to Pseudomonas, Oceanospirillum or Deleya genera. Among these strains, only four were able to degrade APE9-10 (TRITON X 100). They were related to the Pseudomonas genus and were of marine origin. Pure cultures performed with these strains on TRITON X 100 gave APE5 and APE4 as end products. These products were further degraded to APE2 by two other strains unable to degrade the initial surfactant.
Nguyen
M H
MH
Laboratoire de Microbiologie, Faculté des Sciences et Techniques de Saint-Jérôme, Marseille, France.
Sigoillot
J C
JC
D001673:Biodegradation, Environmental; D004987:Ethers / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D015394:Molecular Structure; D017830:Octoxynol / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*; D012722:Sewage; D013055:Spectrophotometry, Infrared; D013501:Surface-Active Agents / Q000378:metabolism*; D014871:Water Microbiology; D014876:Water Pollution
D004987:Ethers; D010636:Phenols; D012722:Sewage; D013501:Surface-Active Agents; C023354:alkylpolyethoxylates; D011092:Polyethylene Glycols; D017830:Octoxynol