Multiplexing of Y chromosome specific STRs and performance for mixed samples.
A combination of four Y-specific polymorphic STR loci was amplified simultaneously using fluorescently labeled primers. Multiplex conditions required optimization to eliminate constant bands and amplification products for female DNA. A series of experiments was carried out for mixtures of DNA from two males, and from male and female individuals for the Y-specific STRs and an autosomal locus. For the male/male mixtures amplified with the Y specific system, and amplified for an autosomal locus, the minor component in the mixture could only be identified up to a ratio of 1:10, 1:50 respectively. In male/female DNA mixtures the Y STR alleles could be identified for the highest ratio tested, 400 pg male in DNA in 800 ng female DNA which amounts to a ratio of 1:2000.
Prinz
M
M
Department of Forensic Biology, Office of the Chief Medical Examiner, New York, NY 10022, USA.
Boll
K
K
Baum
H
H
Shaler
B
B
D000483:Alleles; D001826:Body Fluids / Q000378:metabolism*; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D004586:Electrophoresis; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012732:Sex Determination Analysis / Q000379:methods; D014998:Y Chromosome
D017931:DNA Primers; D004247:DNA