A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator.
The bacterial cloning vector, pGreenscript A, derived from the mutated Aequorea green fluorescent protein (GFP-S65A) gene, when expressed in E. coli produced colonies that showed yellow color under daylight and strong green fluorescence under long-wave ultraviolet light. The vector was used to select for inserted foreign genes based on the loss of the yellow color/green fluorescence of E. coli cells caused by the insertional inactivation of GFP production.
Inouye
S
S
Scripps Institution of Oceanography, Marine Biology Research Division, University of California at San Diego, 92093, USA.
Ogawa
H
H
Yasuda
K
K
Umesono
K
K
Tsuji
F I
FI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D037401:Scyphozoa / Q000235:genetics*
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins