title: Relationship of the time of storage and transfusion reactions to platelet concentrates from buffy coats.
abstract: BACKGROUND
  Transfusion reactions to platelet concentrates prepared from buffy coats (BC-PCs) were reviewed to determine the effect of some variables of BC-PC preparation and storage: time of BC storage before BC-PC preparation (1-2 days); time of BC-PC storage before transfusion (1-5 days); no white cell reduction versus laboratory and bedside BC-PC white cell reduction.
  
  
  METHODS
  A multiple linear logistic regression model was used by which the relative effect of one variable is expressed as the relative risk of transfusion reaction against a baseline level (1-day storage, no white cell reduction).
  
  
  RESULTS
  During the 14 months of study, a total of 2707 BC-PC transfusions were given to 192 patients; 37 reactions (1.4%) were reported in 25 patients (13%). The transfusion reactions were febrile, nonhemolytic in 23 cases; allergic in 5; febrile and allergic in 2; and other in 7. The relative risk of transfusion reaction to BC-PCs prepared from BCs stored for 2 days was 1.98 times that to BC-PCs prepared from BCs stored for 1 day (p = 0.07). The relative risk of transfusion reaction of 5-day-old BC-PCs was 10.7 times that of 1-day-old BC-PCs (p = 0.001). The relative risk of transfusion reactions of BC-PCs white cell-reduced in the laboratory and at the bedside were 0.65 (p = 0.3) and 1.87 (p = 0.1) times, respectively, that of non-white cell-reduced BC-PCs.
  
  
  CONCLUSIONS
  Time of storage seems to be an important variable associated with BC-PC transfusion reaction.
authors.affiliation: Centro Trasfusionale e di Immunologia dei Trapianti, Istituto di Ricovero e Cura a Carattere Scientifico Ospedale Maggiore, Milan, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Riccardi
authors.forename: E
authors.initials: E
authors.lastname: Raspollini
authors.forename: P
authors.initials: P
authors.lastname: Rebulla
authors.forename: M
authors.initials: M
authors.lastname: Pappalettera
authors.forename: F
authors.initials: F
authors.lastname: Marangoni
authors.forename: N
authors.initials: N
authors.lastname: Greppi
authors.forename: G
authors.initials: G
authors.lastname: Sirchia
mesh_terms: D016913:Blood Component Transfusion / Q000009:adverse effects*; D001792:Blood Platelets / Q000166:cytology*; D001800:Blood Specimen Collection; D002470:Cell Survival; D006801:Humans; D007962:Leukocytes / Q000166:cytology*; D017713:Platelet Transfusion / Q000009:adverse effects*; D011786:Quality Control; D012189:Retrospective Studies