title: Identification of a lactoferrin-binding protein in Prevotella nigrescens.
abstract: A 40-kDa lactoferrin-binding protein was identified in a strain of Prevotella nigrescens isolated from a patient with periodontitis. The protein was purified by affinity column chromatography using a Sepharose-lactoferrin column and detergent-solubilized membranes. The N-terminal sequence revealed no apparent similarities with any other sequenced bacterial protein. The native conformation of the 40-kDa protein was a condition to bind either iron-free or iron-saturated lactoferrin. A possible function of this Lf-binding protein could be related with an iron acquisition mechanism in P. nigrescens.
authors.affiliation: Department of Functional Biology (Microbiology), Faculty of Medicine, University of Oviedo, Spain.
authors.forename: A
authors.initials: A
authors.lastname: de Lillo
authors.forename: J F
authors.initials: JF
authors.lastname: Fierro
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins; D016866:Bacteroidaceae Infections / Q000382:microbiology; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007781:Lactoferrin / Q000378:metabolism*; D008970:Molecular Weight; D010518:Periodontitis / Q000382:microbiology; D018720:Prevotella / Q000737:chemistry*; D011489:Protein Denaturation; D017421:Sequence Analysis
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C027768:lactoferrin binding proteins, bacterial; D007781:Lactoferrin