title: Amyloid beta protein and transthyretin, sequestrating protein colocalize in normal human kidney.
abstract: The localization of amyloid beta protein (A beta), A beta 40, A beta 42, and transthyretin (TTR) was investigated immunohistochemically in the autopsied human kidney, using polyclonal antibodies against TTR, A beta and C-terminal end-specific antibodies against A beta 40 and 42. Immunoreactivities of A beta and A beta 40 were found both in the proximal and distal tubular epithelial cells. But the immunolocalization of A beta 40 was observed predominantly in the distal tubules whereas that of A beta 42 was predominantly recognized in the proximal tubules. TTR, sequestrating protein for A beta, was present in the proximal tubules. The mechanism by which A beta does not form amyloid in Alzheimer's disease outside the brain remains unknown. The tubular epithelial cells in the kidney may provide a useful system to shed light on this issue.
authors.affiliation: Department of Microbiology, Sapporo Medical University, School of Medicine, Japan. kayo@sapmed.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Tsuzuki
authors.forename: R
authors.initials: R
authors.lastname: Fukatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: N
authors.initials: N
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Takamaru
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Tateno
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: N
authors.initials: N
authors.lastname: Takahata
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis*; D016564:Amyloid beta-Protein Precursor / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000737:chemistry*; D011228:Prealbumin / Q000032:analysis*; D012016:Reference Values
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D011228:Prealbumin