Overexpression of the gene of ribosomal protein L30 from Thermus thermophilus and crystallization of the recombinant protein.
Ribosomal protein L30 from Thermus thermophilus was overexpressed in E. coli cells. The recombinant protein was isolated and crystallized. The crystals belong to the spatial group P3(1) 12, and their crystallographic parameters are not different from those of crystals obtained earlier from the ribosomal protein isolated from T. thermophilus.
Khairullina
A R
AR
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Shcherbakov
D V
DV
Tishchenko
S V
SV
Nikonov
S V
SV
Garber
M B
MB
D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D015345:Oligonucleotide Probes / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000235:genetics* / Q000302:isolation & purification*; D016963:Thermus thermophilus / Q000235:genetics*
D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C035201:ribosomal protein L30