title: Influenza-specific immunity induced by recombinant Listeria monocytogenes vaccines.
abstract: In this study, we evaluate two Listeria monocytogenes strains that express influenza nucleoprotein (NP) sequences for their ability to protect against challenge with influenza-virus. The construction of one strain, which expresses only the Kd restricted NP epitope (NP 147-155), is described in this study; the other strain, which expresses the full NP sequence in the form of a fusion protein, has been described previously. The ability of the two strains to present the Kd restricted NP epitope in vitro and induce NP-specific CTL in vivo is also described. Mice immunized by the intravenous route with either strain cleared a subsequent (3 weeks post-immunization) influenza virus infection more rapidly as indicated by reduced virus titers in the lungs 5 days after challenge. Efficacy of both recombinant L. monocytogenes strains as vaccines in this system was equivalent and equal to that of recombinant vaccinia expressing NP.
authors.affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
authors.forename: G
authors.initials: G
authors.lastname: Ikonomidis
authors.forename: D A
authors.initials: DA
authors.lastname: Portnoy
authors.forename: W
authors.initials: W
authors.lastname: Gerhard
authors.forename: Y
authors.initials: Y
authors.lastname: Paterson
mesh_terms: D000818:Animals; D002460:Cell Line; D000939:Epitopes / Q000378:metabolism; D007113:Immunity, Innate; D009980:Influenza A virus / Q000276:immunology* / Q000378:metabolism; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology*; D008168:Lung / Q000821:virology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control* / Q000628:therapy; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology / Q000378:metabolism; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000939:Epitopes; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014758:Viral Core Proteins; D014765:Viral Vaccines