In a vaccine model, selected substitution of a highly stimulatory T cell epitope of hen's egg lysozyme into a Salmonella flagellin does not result in a homologous, specific, cellular immune response and may alter the way in which the total antigen is processed.
A 13 amino acid peptide corresponding to a potent BALB/c mouse T cell epitope of hen's egg lysozyme (HEL) was substituted singly at five sites in the d flagellin of Salmonella muenchen. The resulting chimeric proteins were unable to expand T cells capable of being stimulated by the HEL epitope and induced T cell populations which either failed to respond or responded at a low level to a normally highly stimulatory flagellin T cell epitope that was present in all chimeras. The results suggested that substitution of a T cell epitope in flagellin may alter the processing of the resulting immunogen.
Vanegas
R A
RA
Department of Microbiology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.
Street
N E
NE
Joys
T M
TM
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000939:Epitopes / Q000276:immunology* / Q000494:pharmacology; D005408:Flagellin / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009113:Muramidase / Q000276:immunology* / Q000494:pharmacology; D012475:Salmonella / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014436:Typhoid-Paratyphoid Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology
D000939:Epitopes; D014436:Typhoid-Paratyphoid Vaccines; D014614:Vaccines, Synthetic; D005408:Flagellin; D009113:Muramidase