Polyamine transport systems of Leishmania donovani promastigotes.
The following observations are conjointly indicative of the presence of distinct energy-dependent, saturable and multiple polyamine transport systems in Leishmania donovani promastigotes, the causative agent for visceral leishmaniasis. Spermidine was influxed with as much as seven times higher rate than putrescine, while both spermidine and putrescine transporters exhibited equally high affinity for the respective polyamine. N-Ethylmaleimide arrested the complete functionality of both the transporters which could be restored by reduced glutathione. Putrescine transporter did not recognize spermine but spermidine was recognized to some extent, while spermidine transporter significantly recognized spermine but putrescine was absolutely spared. A few aromatic diamines viz., diaminobiphenyl and the analogs as well as aliphatic diamines viz., cadaverine and agmatine were selectively recognized by the putrescine transporter only. L. donovani promastigotes grown in presence of alpha-difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase, registered marked upregulation of putrescine transport while spermidine transport was only marginally induced. PA transport systems provide the alternative pool of polyamines in L. donovani promastigotes in the absence of an adequate intracellular PA repertoire.
Kandpal
M
M
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Tekwani
B L
BL
D000596:Amino Acids / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism / Q000493:pharmacokinetics*; D001692:Biological Transport / Q000187:drug effects; D003959:Diamines / Q000378:metabolism / Q000493:pharmacokinetics; D000518:Eflornithine / Q000494:pharmacology; D007893:Leishmania donovani / Q000187:drug effects / Q000378:metabolism*
D000596:Amino Acids; D015317:Biogenic Polyamines; D003959:Diamines; D000518:Eflornithine