Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells.
Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was found to be depressed after several days incubation. Cellular alkaline phosphatase activity followed a similar pattern: an initial increase induced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decrease in this parameter. 45Ca2+ uptake was only significantly inhibited by advanced glycation end-products after 24 h incubation. These results suggest that advanced glycation end-products directly regulate osteoblast proliferation and differentiation in a dose and time dependent manner.
McCarthy
A D
AD
Cátedra de Bioquímica Patólogica, Universidad Nacional de La Plata, Argentina.
Etcheverry
S B
SB
Bruzzone
L
L
Cortizo
A M
AM
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D017127:Glycation End Products, Advanced / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012516:Osteosarcoma; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D017127:Glycation End Products, Advanced; C081628:advanced glycation end products-bovine serum albumin; D012710:Serum Albumin, Bovine; D000469:Alkaline Phosphatase; D002118:Calcium