Preparation of polymeric urease discs by an electron beam irradiation technique.
A preparation method of immobilized urease discs by an electron beam irradiation technique was developed, and the relationship between enzyme activity and preparation conditions was investigated. The immobilized urease disc was a thin circular film (200 microm, 5 mm phi) that is useful for biomedical applications. The activity of urease irradiated with 1 Mrad at room temperature was protected by the presence of cysteine. The activity of the immobilized urease discs was studied as a function of monomer concentration (80-90%) and the thicker disc gave a high activity. The durability of the immobilized urease discs gave a high activity. The durability of the immobilized urease discs was evaluated by repeated batch enzyme reactions, and a high activity yield (80-85%) was obtained.
Higa
O Z
OZ
Comissao Nacional De Energia Nuclear, CNEN/SP, Cidade Universitaria, Armando De Salles Oliveira, Sao Paulo, Brazil.
Kumakura
M
M
D003545:Cysteine / Q000737:chemistry / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004800:Enzymes, Immobilized; D007700:Kinetics; D010209:Paper; D010315:Particle Accelerators; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects*; D011485:Protein Binding; D014510:Urease / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects*
D004800:Enzymes, Immobilized; D011108:Polymers; D014510:Urease; D003545:Cysteine