Expression of Tetrahymena snRNA gene variants including a U1 gene with mutations in the 5' splice site recognition sequence.
The expression of U1, U2 and U5 snRNA gene variants has been studied under different physiological states of Tetrahymena. Variants of all three snRNA genes are expressed. Among the snRNAs detected is U1-3, a variant with 66 mutations compared to the normal U1 snRNA. Three of these mutations affect the 5' splice site recognition sequence. The U1-3 snRNA is present in a few hundred copies per cell. The expression of Tetrahymena snRNA genes is independent of the physiological state of the cell.
Eugen-Olsen
J
J
Institute of Medical Biochemistry and Genetics, The Panum Institute, University of Copenhagen, Denmark.
Hagemeister
J J
JJ
Hellung-Larsen
P
P
D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation / Q000502:physiology; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000235:genetics*; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000737:chemistry / Q000235:genetics*; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000235:genetics; D017415:Ribonucleoprotein, U5 Small Nuclear / Q000235:genetics; D016808:Tetrahymena thermophila / Q000235:genetics* / Q000502:physiology
D012342:RNA, Small Nuclear; D017412:Ribonucleoprotein, U1 Small Nuclear; D017413:Ribonucleoprotein, U2 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear