title: Collection of peripheral blood stem cells from normal donors 60 years of age or older.
abstract: We report 14 normal peripheral blood stem cell (PBSC) donors > or = 60 years of age who had cytokine mobilization followed by PBSC apheresis for allogeneic transplantation. Mobilization was achieved with filgrastim (6 microg/kg twice daily). Their median age was 63.5 years (range 60-77), and 43% had a positive medical history, mainly hypertension and/or cardiac problems. Their median pre-apheresis leucocyte count (x 10(9)/l) was 38.6 (range 29.6-63.4). The median apheresis yield (x 10(6) CD34+ cells/litre blood processed, first apheresis) was 27.9 (range 1.6-54.8). The target cell dose (> or = 4 x 10(6) CD34+ cells/kg recipient) was reached with one procedure in eight (57%) donors. Filgrastim-related adverse events were acceptable and apheresis was well tolerated. When compared to younger donors (< 60 years of age), a trend to a lower CD34+ apheresis yield and a requirement for more than one apheresis to achieve the collection target (> or = 4 x 10(6) CD34+ cells/kg) was evident. Although older (> or = 60 years) donors seem to mobilize less effectively, these data suggest that PBSC collection from them is feasible and has an acceptable short-term safety profile.
authors.affiliation: Department of Hematology, The University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
authors.forename: P
authors.initials: P
authors.lastname: Anderlini
authors.forename: D
authors.initials: D
authors.lastname: Przepiorka
authors.forename: J
authors.initials: J
authors.lastname: Lauppe
authors.forename: D
authors.initials: D
authors.lastname: Seong
authors.forename: S
authors.initials: S
authors.lastname: Giralt
authors.forename: R
authors.initials: R
authors.lastname: Champlin
authors.forename: M
authors.initials: M
authors.lastname: Körbling
mesh_terms: D000368:Aged; D005260:Female; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D006801:Humans; D007937:Leukapheresis; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000069585:Filgrastim