[Characterization of wool dust extract in vitro].
Wool dust has been described as a cause of respiratory impairment in workers occupationally exposed in wool processing. A pharmacological investigation was performed on 30 samples of isolated guinea plg trachea with wool dust water extracts prepared from the material collected at the workplace of exposed workers. Wool extract was added into the system with Krebs solution in progressively increased concentrations of 10, 30, 100, 300 and 1000 microliters. The constriction of the tracheal smooth muscle caused by wool dust extract was diminished after pretreatment with pyrilamine (antihistamine) and verapamil (intracellular and extracellular calcium blocking agent). Atropine (anticholinergic) and indomethacin (inhibitor of prostaglandin synthesis) had very little impact on the bronchoconstrictive effect of wool dust extract on the tracheal smooth muscle. This finding suggests that the airway constriction induced by wool dust is party attributable to mediators like histamine and also to calcium.
Zuskin
E
E
Skola narodnog zdravlja Andrija Stampar Medicinskog fakulteta Sveucilista u Zagrebu.
Mustajbegović
J
J
D000818:Animals; D004391:Dust / Q000009:adverse effects*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D014132:Trachea / Q000502:physiology*; D014935:Wool
D004391:Dust
Karakteriziranje ekstrakta vunene prasine in vitro.