A novel putative G-protein-coupled receptor expressed in lung, heart and lymphoid tissue.
cDNA encoding a novel putative G-protein-coupled receptor, named LyGPR (lymphocyte derived G-protein-coupled receptor) was cloned using a reverse transcription-PCR approach. The LyGPR amino acid sequence is 375 residues long and shows similarity (about 30-35% identity) both to the angiotensin receptors and members of the chemokine receptor family. Northern blot analysis revealed a 3.1-kb LyGPR transcript expressed predominantly in lung, heart and lymphoid tissues. LyGPR expression was also detected in the pre-B acute lymphoblastoid leukemia cell lines Reh and Nalm-6, in the Burkitt's lymphoma line Daudi, and in hematopoietic progenitor cells from bone marrow, as well as in B cells, T cells and monocytes from peripheral blood.
Kvingedal
A M
AM
Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo. a.m.kvingedal@labmed.uio.no
Smeland
E B
EB
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000737:chemistry*; D008221:Lymphoid Tissue / Q000737:chemistry*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014018:Tissue Distribution
D018076:DNA, Complementary; D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins