title: Beta 2-microglobulin removal by synthetic dialysis membranes. Mechanisms and kinetics of the molecule.
abstract: Beta 2-microglobulin (beta 2-m) accumulation represents a possible complication of long term dialysis. It is therefore important to evaluate the capacity of removal of this molecule from the patient by different dialysis membranes. The present study is aimed at evaluating the mechanisms involved in beta 2-m removal by three different synthetic membranes: a) highly asymmetric hydrophobic polysulfone (Biosulfane, NMC), b) moderately asymmetric and hydrophobic polysulfone (PS600, Fresenius), c) Polyacylonitrile (AN69HF, Hospal). The adsorption capacity and sieving coefficients of the three membranes for native and labeled beta 2-m were studied in vitro utilizing human blood. The amount adsorbed by the membrane was measured by the elution of the molecule obtained with a detergent solution. Clearances, total removal and membrane adsorption were studied in six patients treated in a randomized sequence with the three membranes. For this purpose, plasma and dialysate measurements as well as total collection of spent dialysate and beta 2-m elution from the used dialyzers were carried out. Ex novo generation of beta 2-m did not take place during in vitro circulation. The molecule was removed by the studied membranes both by filtration and adsorption. The Biosulfane membrane removed beta 2-m mostly by adsorption while the PS600 membrane removed beta 2-m almost entirely by filtration. Intermediate behaviour was shown by AN69 membrane. Similar quantities of beta 2-m were removed from the patients with the three membranes. Total removal could only be precisely measured by adding the quantity of beta 2-m eluted from the membrane to the amount recovered in the spent dialysate. Out of total removal, adsorption was more than 90% with Biosulfane, while only 5% with the PS600. These findings contribute to the understanding of the discrepancy found between the clearance measured from the plasma side and that measured from the dialysate side. In conclusion, clearance and sieving measurements for beta 2-m cannot be correctly performed unless the capacity of adsorption of the membrane is taken into account.
authors.affiliation: Department of Nephrology, St. Bortolo Hospital, Vicenza, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Ronco
authors.forename: A
authors.initials: A
authors.lastname: Heifetz
authors.forename: K
authors.initials: K
authors.lastname: Fox
authors.forename: C
authors.initials: C
authors.lastname: Curtin
authors.forename: A
authors.initials: A
authors.lastname: Brendolan
authors.forename: F
authors.initials: F
authors.lastname: Gastaldon
authors.forename: C
authors.initials: C
authors.lastname: Crepaldi
authors.forename: A
authors.initials: A
authors.lastname: Fortunato
authors.forename: G
authors.initials: G
authors.lastname: Pietribasi
authors.forename: A
authors.initials: A
authors.lastname: Caberlotto
authors.forename: A
authors.initials: A
authors.lastname: Brunello
authors.forename: S
authors.initials: S
authors.lastname: Milan Manani
authors.forename: M
authors.initials: M
authors.lastname: Zanella
authors.forename: G
authors.initials: G
authors.lastname: La Greca
mesh_terms: D000180:Acrylic Resins / Q000378:metabolism*; D000181:Acrylonitrile / Q000031:analogs & derivatives* / Q000378:metabolism; D000327:Adsorption; D001672:Biocompatible Materials / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D006400:Hematocrit; D006801:Humans; D066298:In Vitro Techniques; D007676:Kidney Failure, Chronic / Q000628:therapy*; D008567:Membranes, Artificial; D011108:Polymers / Q000378:metabolism*; D006435:Renal Dialysis / Q000009:adverse effects / Q000592:standards*; D013450:Sulfones / Q000378:metabolism*; D014508:Urea / Q000652:urine; D001613:beta 2-Microglobulin / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D001798:Blood Proteins; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; D001613:beta 2-Microglobulin; C017662:polysulfone P 1700; C016892:AN-69; D014508:Urea; D000181:Acrylonitrile