Administration of corticosterone alters intracellular localization of brain-derived neurotrophic factor-like immunoreactivity in the rat brain.
We investigated the distribution of immunoreactivity for brain-derived neurotrophic factor (BDNF) in rat brain after peripheral administration of corticosterone or vehicle. In the immunohistochemical study, BDNF-like immunoreactivity (LI) was observed predominantly in the nucleus of the cortical and hippocampal neurons in the brain of vehicle-treated rats. In corticosterone-treated rats, BDNF-LI was markedly reduced in the nucleus and concomitantly increased in cytoplasm. Western immunoblot study also demonstrated that corticosterone significantly reduced BDNF-LI in the nuclear fraction of the cerebral cortex and hippocampus. These results indicate that corticostrone regulates the intracellular localization of BDNF and/or its derivatives in the rat brain.
Nitta
A
A
Laboratory of Molecular Biology, Gifu Pharmaceutical University, Japan.
Fukumitsu
H
H
Kataoka
H
H
Nomoto
H
H
Furukawa
S
S
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003345:Corticosterone / Q000494:pharmacology*; D006624:Hippocampus / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar
D019208:Brain-Derived Neurotrophic Factor; D003345:Corticosterone