A simple method for enumerating bacteriophages in soil.
A plaque technique that uses antibiotic-resistant bacteria growing on antibiotic-containing agar for the assay lawn resulted in significantly better recovery of bacteriophages P1 of Escherichia coli and F116 of Pseudomonas aeruginosa from nonsterile soil than standard membrane filtration or centrifugation techniques. Adsorption of the phages on soil particles appeared to be involved in their recovery and survival in soil.
Yin
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Department of Biology, New York University, New York 1003, USA.
Zeph
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Stotzky
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D000327:Adsorption; D017101:Bacteriophage P1 / Q000254:growth & development; D001435:Bacteriophages / Q000254:growth & development*; D002498:Centrifugation; D005069:Evaluation Studies as Topic; D017105:Pseudomonas Phages / Q000254:growth & development; D012988:Soil Microbiology; D010948:Viral Plaque Assay