Rapid and selective analysis of secnidazole in human plasma using high-performance liquid chromatography with ultraviolet detection.
A rapid, reproducible high-performance liquid chromatographic method for the determination of secnidazole, 5-nitroimidazole class of antiprotozoals from blood is described. Metronidazole was used as an internal standard. A simple extraction step with dichloromethane was done before chromatography on a C18 column with the wavelength fixed at 276 nm on the UV detector. Blood levels up to 500 ng/ml have been measured with good precision in the healthy volunteers after 1 g of secnidazole was administered. The present described method can readily be utilized for routine pharmacokinetic studies.
Ravi
S K
SK
Department of Clinical Pharmacology and Therapeutics, Nizam's Institute of Medical Sciences, Panjagutta, Hyderabad, India.
Naidu
M U
MU
Sekhar
E C
EC
Rao
T R
TR
Shobha
J C
JC
Rani
P U
PU
Surya
K J
KJ
D000981:Antiprotozoal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008795:Metronidazole / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000981:Antiprotozoal Agents; D008795:Metronidazole; C016724:secnidazole