title: Histochemical study of lectin binding in the major salivary glands of adult fallow-deer (Dama dama L.).
abstract: The sugar residues in glycoconjugates present in the parotid and mandibular glands of the adult fallow-deer were detected and characterized by using a battery of eight different lectin-horseradish peroxidase conjugates. In some cases a treatment with sialidase preceded the lectin staining. Parotid secretory cells produced glycoconjugates with N-acetylgalactosamine, N-acetylglucosamine and mannose residues. Mucous acinar cells were the most reactive sites of the mandibular gland and contained conspicuous quantities of oligosaccharides with terminal sialic acid radicals. Galactosil-(beta 1-->3)N-acetylgalactosamine was the most abundant penultimate sugar linked to N-acetylneuraminic acid. Mandibular mucous cells also presented N-acetylglucosamine and sialylated components with the terminal dimer sialic acid-N-acetylgalactosamine. Demilunar cells contained glycoconjugates with fucose and mannose residues. The apical surface of duct cells was stained by all the lectins.
authors.affiliation: Department of Anatomy for Domestic Animals, Faculty of Veterinary Medicine, Matelica, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Pedini
authors.forename: P
authors.initials: P
authors.lastname: Ceccarelli
authors.forename: A M
authors.initials: AM
authors.lastname: Gargiulo
authors.forename: C
authors.initials: C
authors.lastname: Dall'Aglio
mesh_terms: D000818:Animals; D001665:Binding Sites; D003670:Deer; D005260:Female; D006651:Histocytochemistry; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D010306:Parotid Gland / Q000166:cytology / Q000378:metabolism; D012469:Salivary Glands / Q000166:cytology* / Q000378:metabolism*
chemical_list: D037102:Lectins