Maximizing flap survival in a prefabrication model using exogenous and endogenous bFGF: a new approach.
Flap prefabrication is dependent on the neovascular response that occurs between the implanted arteriovenous pedicle and the recipient tissue. Augmentation of this neovascular response with angiogenic growth factors would maximize flap survival and minimize the interval between pedicle implantation and flap rotation. Maximizing the biologic activity of endogenous growth factors would likewise positively impact upon flap survival. This study examined the role of basic fibroblast growth factor, a known potent angiogenic growth factor, on flap survival in a rabbit ear prefabrication model. Sucrose octasulfate, a substance that binds basic fibroblast growth factor, stabilizes it, and protects it from degradation, was also studied to determine its impact on flap survival. Flap survival was increased using basic fibroblast growth factor, sucrose octasulfate, and the two substances combined together. The use of substrates designed to maximize the biologic activity of endogenous growth factors, rather than relying on the artificial addition of exogenous growth factors, represents a new approach in the search for methods that will improve flap survival.
Duffy
F J
FJ
Division of Plastic Surgery, Brigham and Women's Hospital, Boston, MA 02115-6195, USA.
Maitz
P K
PK
Hergrueter
C A
CA
Pribaz
J J
JJ
D000818:Animals; D004431:Ear, External / Q000601:surgery; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism* / Q000494:pharmacology*; D006085:Graft Survival; D011817:Rabbits; D013395:Sucrose / Q000031:analogs & derivatives / Q000494:pharmacology; D013524:Surgical Flaps
D016222:Fibroblast Growth Factor 2; D013395:Sucrose; C053081:sucrose octasulfate