Prostaglandin F2alpha receptor (FP receptor) agonists are potent adipose differentiation inhibitors for primary culture of adipocyte precursors in defined medium.
Prostaglandin F2alpha inhibits adipose differentiation of primary culture of adipocyte precursors and of the adipogenic cell line 1246 in defined medium. In the present paper, we investigated the effect of FP receptor agonists cloprostenol and fluprostenol on the differentiation of newborn rat adipocyte precursors in primary culture. The results show that cloprostenol and fluprostenol are very potent inhibitors of adipose differentiation. Dose response studies indicate that both agonists are more potent than PGF2alpha in inhibiting adipocyte precursors differentiation. 50% inhibition of adipose differentiation was observed at a concentration of 3 x 10(-12) M for cloprostenol and 3 to 10 x 10(-11) M for fluprostenol respectively whereas the PGF2alpha concentration required to elicit the same effect was 10(-8) M. In contrast compounds structurally related to PGE2 such as 17-phenyl trinor PGE2 had no effect on adipose differentiation except when added at a 10,000-fold higher concentration.
Serrero
G
G
Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore 21201-1180, USA. gserrero@pharmacy.ab.umd.edu
Lepak
N M
NM
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003008:Cloprostenol / Q000494:pharmacology; D003470:Culture Media; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011982:Receptors, Prostaglandin / Q000819:agonists*
D003470:Culture Media; D011461:Prostaglandins F, Synthetic; D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor; C006326:fluprostenol; D003008:Cloprostenol; D005993:Glycerolphosphate Dehydrogenase