Determination of radiosensitivity in established and primary squamous cell carcinoma cultures using the micronucleus assay.
In this study, the cytokinesis-block micronucleus assay (CBMN) was used to measure radiosensitivity in three established cell lines (SCC-61, V175 and V134) and 10 primary cell cultures of squamous cell carcinoma (SCC) of the head and neck. Assessment involved optimisation of the assay to determine cytochalasin-B (CB) concentration and sampling time postirradiation. A much closer correlation between dose-response data measured in the clonogenic and micronucleus assays was found when the micronucleus assay was performed under standardised conditions for each cell line (2 micrograms/ml CB: 48 h postirradiation) instead of predetermined optimised assay conditions. This indicates that, for these SCC cell lines, the CBMN assay may be able to predict in vitro radiosensitivity. To be of clinical use in predicting radiosensitivity, the CBMN assay also needs to be evaluated with primary cell cultures. In this study, no relationship between micronucleus frequency at 2 or 6 Gy and patient clinical outcome 12 months following surgery and radiotherapy was seen. Similarly, no association between patient outcome and tumour stage, nodal stage and histology was observed. These CBMN assay data from the primary cell cultures are presently inconclusive as a measure of patient tumour radiosensitivity.
Champion
A R
AR
Cellular and Molecular Radiation Research Unit, Velindre Hospital NHS Trust, Whitchurch, Cardiff, U.K.
Hanson
J A
JA
Venables
S E
SE
McGregor
A D
AD
Gaffney
C C
CC
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002470:Cell Survival / Q000528:radiation effects; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D015162:Micronucleus Tests; D014411:Neoplastic Stem Cells / Q000528:radiation effects; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D004273:DNA, Neoplasm