title: Reactivity of murine and human recombinant LPS-binding protein (LBP) within LPS and gram negative bacteria.
abstract: The serum lipopolysaccharide (LPS) binding protein, LBP, has been shown to greatly enhance cellular responses to low concentrations of LPS. Purified LBP facilitates the transfer of LPS to membrane-bound or soluble CD14; the CD14/LPS complex then triggers a signal in responsive cells. We have cloned and sequenced a cDNA encoding murine LBP, and produced recombinant murine LBP using a baculovirus expression system. Using either a solid-phase or a cytofluorometric assay, recombinant murine and human LBP were found to bind avidly to free LPS, but only weakly to live bacteria from most LPS-containing Gram negative strains. Binding correlated loosely with the length and composition of the polysaccharide O chains. However, recombinant LBP did bind well to all heat-killed bacterial preparations. These findings suggest that LBP could be implicated in the response to killed but not live Gram negative bacteria.
authors.affiliation: Division of Infectious Diseases, CHUV, Laussane, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Lengacher
authors.forename: C V
authors.initials: CV
authors.lastname: Jongeneel
authors.forename: D
authors.initials: D
authors.lastname: Le Roy
authors.forename: J D
authors.initials: JD
authors.lastname: Lee
authors.forename: V
authors.initials: V
authors.lastname: Kravchenko
authors.forename: R J
authors.initials: RJ
authors.lastname: Ulevitch
authors.forename: M P
authors.initials: MP
authors.lastname: Glauser
authors.forename: D
authors.initials: D
authors.lastname: Heumann
mesh_terms: D000209:Acute-Phase Proteins; D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D018076:DNA, Complementary; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D000099231:Lipopolysaccharide-Binding Protein