Ultrastructure of atheromatous lesions experimentally induced in Syrian hamsters of the APA strain.
In order to examine whether diabetes enhances, primary aortic lesions up to atherosclerotic ones, mild primary lesions were induced in aorta of APA hamsters by an administration of vitamin D2 (VD) and/or stop-and-reflow (SR)-operation, a modification of renal artery clamping. At 2 months after the treatment with the combination of VD-administration and SR-operation, atheromatous lesions, characterized by an appearance of many foam cells in the intima, were observed in the abdominal aorta, the site of SR-operation, in streptozotocin (SZ)-induced diabetic APA hamsters. Foam cells in the atheromatous lesions were originated from smooth muscle cells and monocyte/ macrophages. On the other hand, neither VD-administration alone nor SR-operation alone developed atheromatous lesions in SZ-induced diabetic APA hamsters. In conclusion, we succeeded in a rapid induction of atherosclerotic lesions in abdominal aorta of SZ-induced diabetic APA hamsters by the combination of VD-administration and SR-operation.
Yamanouchi
J
J
Department of Biomedical Science, University of Tokyo, Japan.
Sugawara
Y
Y
Itagaki
S
S
Doi
K
K
D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology; D001161:Arteriosclerosis / Q000097:blood / Q000473:pathology*; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D002784:Cholesterol / Q000097:blood; D006224:Cricetinae; D004730:Endothelium, Vascular / Q000473:pathology / Q000648:ultrastructure; D004872:Ergocalciferols / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D004872:Ergocalciferols; D014280:Triglycerides; D002784:Cholesterol