Inhibition of motility and invasion of B16 melanoma by the overexpression of cystatin C.
Although increased expression of cysteine proteinases has been shown to be correlated with increased metastasis for a wide variety of tumours, the contribution of cysteine proteinases to the metastatic spread of tumour cells is not well understood. In order to examine this question we have overexpressed a specific cysteine proteinase inhibitor, cystatin C, by stable transfection of B16F10 melanoma. Increased expression of cystatin C inhibited motility and in vitro invasiveness of B16 melanoma by 50% in both stimulated (autocrine motility factor, laminin) and unstimulated cells. These results suggest that cysteine proteinases are involved in B16 melanoma motility and invasion.
Sexton
P S
PS
Department of Biochemistry, Kirksville College of Osteopathic Medicine, MO 63501, USA.
Cox
J L
JL
D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement / Q000187:drug effects; D055316:Cystatin C; D015891:Cystatins / Q000096:biosynthesis*; D015853:Cysteine Proteinase Inhibitors / Q000096:biosynthesis*; D005956:Glucose-6-Phosphate Isomerase / Q000494:pharmacology; D007700:Kinetics; D007797:Laminin / Q000494:pharmacology; D008546:Melanoma, Experimental / Q000473:pathology* / Q000503:physiopathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
C525031:Cst3 protein, mouse; D055316:Cystatin C; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D007797:Laminin; D011993:Recombinant Fusion Proteins; D005956:Glucose-6-Phosphate Isomerase