title: Structure of the capsular polysaccharide of Clostridium perfringens Hobbs 5 as determined by NMR spectroscopy.
abstract: The complete primary structure of the capsular polysaccharide of Clostridium perfringens Hobbs 5, an anaerobic bacterium implicated in food poisoning, was determined. The polysaccharide was isolated from C. perfringens Hobbs 5 cells, after deproteination, by ethanol precipitation and by ion-exchange chromatography. The polysaccharide was comprised of glucose, galactose, mannose, N-acetylglucosamine, N-acetylgalactosamine, and glucuronic acid, in equimolar ratios. Sequence and linkage assignments of the glycosyl residues were obtained by NMR spectroscopy, specifically by the combination of two-dimensional homonuclear TOCSY and NOESY experiments and heteronuclear (1H, 13C) multiple-quantum coherence (HMQC, HMQC-COSY, HMQC-TOCSY and HMBC) experiments. Thus, the envelope polysaccharide of C. perfringens Hobbs 5 was found to be a polymer composed of a hexasaccharide repeating unit with the following structure: [formula: see text] This structure is novel among bacterial cell-surface polysaccharides, and it is the first of many serotypically distinct capsular polysaccharides of C. perfringens to be described.
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kalelkar
authors.forename: J
authors.initials: J
authors.lastname: Glushka
authors.forename: H
authors.initials: H
authors.lastname: van Halbeek
authors.forename: L C
authors.initials: LC
authors.lastname: Morris
authors.forename: R
authors.initials: R
authors.lastname: Cherniak
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry* / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003016:Clostridium perfringens / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000737:chemistry; D017421:Sequence Analysis
chemical_list: D009005:Monosaccharides; D009844:Oligosaccharides