Correlation of resistance to the alkaloid lycorine with the degree of suppressiveness in petite mutants of Saccharomyces cerevisiae.
In previous papers (Del Giudice et al. Curr Genet 8:493-497, 1984; Massardo et al. Curr Genet 17:455-457, 1985) we have shown that strains of Saccharomyces cerevisiae that are devoid of mitochondrial DNA (rhoo) are resistant to the alkaloid lycorine isolated from Amaryllis plants, whereas strains containing mitochondrial DNA (rho-, mit-, or rho+) are sensitive to this drug. In addition, we were able to show that the so-called hypersuppressive petites, whose mitochondrial genomes consist of short regions of DNA containing an ori sequence,show intermediate resistance. In this paper, we demonstrate that the degree of suppressiveness of a rho- mutant correlates with the degree of resistance to lycorine.
Del Giudice
A
A
Institut für Biologie IV (Mikrobiologie), Rheinisch-Westfälische Technische Hochschule Aachen, Worringer Weg, D-52056 Aachen, Germany.
Massardo
D R
DR
Manna
F
F
Koltovaya
N
N
Hartings
H
H
Del Giudice
L
L
Wolf
K
K
D000470:Alkaloids / Q000494:pharmacology*; D047151:Amaryllidaceae Alkaloids; D004272:DNA, Mitochondrial / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D009154:Mutation; D010617:Phenanthridines / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic
D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D004272:DNA, Mitochondrial; D010617:Phenanthridines; C015330:lycorine