Characterization of cytotoxic T lymphocytes recognizing autologous tumor cells with plural HLA-class I alleles in a patient with esophageal cancer.
T cell sublines and clones were established from the parental HLA-A2601-restricted cytotoxic T lymphocyte line (KE-4 CTL, HLA-A2601/2402, -B5101/40012, -Cw0102/0302) to investigate their capability to recognize tumor antigens on HLA-class I alleles other than HLA-A2601. Thirty-nine of 52 (75%) sublines or 30 of 83 (36%) clones produced interferon (IFN)-gamma by recognition of the autologous KE-4 tumor cells. Among these 39 CTL sublines, 22, 22, and 2 sublines produced IFN-gamma by recognition of HLA-A2402+, -B5101+, and Cw0102+ allogenic tumor cells, respectively. Four, 8, and 4 of these 30 CTL clones also produced IFN-gamma by recognition of HLA-A2402+, -B5101+, and Cw0102+ allogenic tumor cells, respectively. The other 13 sublines or 53 clones failed to produce IFN-gamma by recognition of the autologous tumor cells, respectively. These results suggest the presence of CTL recognizing shared tumor antigens on autologous tumor cells expressed by plural HLA-class I alleles in a patient with esophageal SCC.
Maeda
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Department of Immunology, Kurume University School of Medicine, Japan.
D000483:Alleles; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000276:immunology*; D001483:Base Sequence; D004938:Esophageal Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D005805:Genes, MHC Class I; D006801:Humans; D008969:Molecular Sequence Data; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm