Detection of respiratory syncytial virus from clinical specimens: comparison between reverse transcription polymerase chain reaction and tissue culture.
RT-PCR was compared with tissue culture to detect RSV from nasopharyngeal aspirates. RT-PCR was more sensitive and specific than tissue culture method. RT-PCR has sensitivity and specificity of 100% and 97%, respectively. The results indicate that RT-PCR can be used for detection of RSV in clinical specimens.
Tantivanich
S
S
Department of Microbiology and Immunology, Faculty of Tropical Medicine Mahidol University, Bangkok, Thailand.
Suphanaranonda
K
K
Balachanda
K
K
Anderson
R
R
D015139:Blotting, Southern; D046508:Culture Techniques; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D017817:Nasal Lavage Fluid / Q000821:virology; D012367:RNA, Viral / Q000032:analysis*; D019105:Random Amplified Polymorphic DNA Technique / Q000592:standards*; D015203:Reproducibility of Results; D018357:Respiratory Syncytial Virus Infections / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics* / Q000254:growth & development; D012680:Sensitivity and Specificity; D014776:Virus Cultivation / Q000592:standards*
D012367:RNA, Viral