Functional expression of the murine Golgi CMP-sialic acid transporter in saccharomyces cerevisiae.
We have functionally expressed the murine Golgi putative CMP-sialic acid transporter in Saccharomyces cerevisiae. Using a galactose-inducible expression system, S. cerevisiae vesicles were able to transport CMP-sialic acid. Transport was dependent on galactose induction and was temperature-dependent and saturable with an apparent Km of 2.9 microM. Transport was inhibited by CMP, and upon vesicle disruption with Triton X-100 parameters were very similar to the previously described CMP-sialic acid transport characteristics observed with mammalian Golgi vesicles. CMP-sialic acid transport induction was specific as no transport of UDP-galactose was observed even though the latter putative transporter has a high degree of amino acid sequence identity with the CMP-sialic acid transporter. Together, the above results demonstrate that the previously described cDNA encoding the putative CMP-sialic acid transporter encodes the transporter protein per se and suggests that this heterologous expression system may be used for further structural and functional studies of other Golgi membrane transporter proteins.
Berninsone
P
P
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester, Massachusetts 01655, USA.
Eckhardt
M
M
Gerardy-Schahn
R
R
Hirschberg
C B
CB
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000378:metabolism; D005822:Genetic Vectors; D006056:Golgi Apparatus / Q000378:metabolism*; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D033704:Nucleotide Transport Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D002352:Carrier Proteins; D008565:Membrane Proteins; D033704:Nucleotide Transport Proteins; C101482:Slc35a1 protein, mouse; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid