title: Demonstration of acid-fast bacilli in buffy coat and bone marrow smear--a diagnostic tool in pulmonary tuberculosis.
abstract: Buffy coat smears prepared from peripheral blood and bone marrow aspirate obtained from proved 60 untreated cases of pulmonary tuberculosis were stained with Ziehl-Neelsen method and thoroughly screened for presence of tubercle bacilli. Acid-fast bacilli were detected in 55% cases in buffy coat, 48.3% cases in bone marrow, 38.3% cases both in buffy coat and bone marrow and 65% cases either in buffy coat or in bone marrow or in both. Considering the fact that demonstration of acid-fast bacilli may not be possible in more than 25-50% of the suspected cases of tuberculosis, these techniques are recommended for evaluation of their utility in establishing diagnosis of tuberculosis, particularly in reference to sputum negative cases of pulmonary tuberculosis and tuberculosis of inaccessible extrapulmonary sites.
authors.affiliation: Department of Pathology, Pt B D Sharma Postgraduate Institute of Medical Sciences, Rohtak.
authors.forename: R
authors.initials: R
authors.lastname: Sen
authors.forename: S
authors.initials: S
authors.lastname: Singh
authors.forename: H P
authors.initials: HP
authors.lastname: Singh
authors.forename: J
authors.initials: J
authors.lastname: Sen
authors.forename: M S
authors.initials: MS
authors.lastname: Yadav
authors.forename: B R
authors.initials: BR
authors.lastname: Arora
mesh_terms: D001431:Bacteriological Techniques; D001853:Bone Marrow / Q000382:microbiology*; D001856:Bone Marrow Examination; D003430:Cross-Sectional Studies; D003906:Developing Countries; D006801:Humans; D015994:Incidence; D007194:India; D007962:Leukocytes / Q000382:microbiology*; D008403:Mass Screening; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000453:epidemiology*