title: Three different GB virus C/hepatitis G virus genotypes. Phylogenetic analysis and a genotyping assay based on restriction fragment length polymorphism.
abstract: The 5'-untranslated region (5'-UTR) sequences of 33 GB virus C/hepatitis G virus (GBV-C/HGV) obtained from different geographic areas were determined through reverse-transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide substitution per site, and construction of phylogenetic trees. The 5'-UTR of GBV-HGV was found to be heterogeneous, with 70.9-99.5% homology. Three distinct phylogenetic branches were observed consistently in all phylogenetic trees. GBV-C is the prototype for one, HGV for another, and there is a new branch which consisted of GBV-C/HGV isolates from Asia. Genotype-specific restriction sites for the restriction enzymes, ScrFI and BsmFI, were identified, and a simple restriction fragment polymorphism analysis was developed for genotyping. These data provide evidence that GBV-C/HGV consists of three different genotypes. Our simple genotyping assay will also provide a tool for epidemiological studies of GBV-C/HGV infection.
authors.affiliation: Second Department of Medicine, Nagoya City University Medical School, Mizuho, Nagoya, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Mukaide
authors.forename: M
authors.initials: M
authors.lastname: Mizokami
authors.forename: E
authors.initials: E
authors.lastname: Orito
authors.forename: K
authors.initials: K
authors.lastname: Ohba
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: R
authors.initials: R
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Hikiji
authors.forename: S
authors.initials: S
authors.lastname: Iino
authors.forename: S
authors.initials: S
authors.lastname: Shapiro
authors.forename: N
authors.initials: N
authors.lastname: Lahat
authors.forename: Y M
authors.initials: YM
authors.lastname: Park
authors.forename: B S
authors.initials: BS
authors.lastname: Kim
authors.forename: T
authors.initials: T
authors.lastname: Oyunsuren
authors.forename: M
authors.initials: M
authors.lastname: Rezieg
authors.forename: M N
authors.initials: MN
authors.lastname: Al-Ahdal
authors.forename: J Y
authors.initials: JY
authors.lastname: Lau
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018067:Flaviviridae / Q000145:classification*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012367:RNA, Viral