title: A 23911 bp region of the Bacillus subtilis genome comprising genes located upstream and downstream of the lev operon.
abstract: Within the framework of the European project to sequence the whole Bacillus subtilis 168 genome, a 23911 bp long chromosomal DNA fragment located around 233 degrees on the B. subtilis genetic map was cloned and sequenced. From the generated sequencing data and the results of the homology search, the primary structure of this region was determined. In addition to the whole lev operon, the region contains putative genes for an amino acid permease, two different alcohol dehydrogenases, a chitosanase, a protein belonging to the LysR family of transcriptional regulators, a protein related to the MerR transcriptional regulator, up to four proteins related to the product of the spoF gene, and genes coding for nine more inferred proteins of unknown function.
authors.affiliation: Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Parro
authors.affiliation: Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: San Román
authors.affiliation: Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Galindo
authors.affiliation: Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix-du-Sud, 2/20, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Purnelle
authors.affiliation: Laboratoire de Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France.
authors.forename: Alexander
authors.initials: A
authors.lastname: Bolotin
authors.affiliation: Laboratoire de Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France.
authors.forename: Alexei
authors.initials: A
authors.lastname: Sorokin
authors.affiliation: Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Rafael P
authors.initials: RP
authors.lastname: Mellado
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D015698:Genomic Library; D006026:Glycoside Hydrolases / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D006026:Glycoside Hydrolases; C021512:levanase