Expression of platelet-activating factor receptor in the hamster oviduct: localization to the endosalpinx.
Platelet-activating factor (PAF) is a lipid mediator that has a range of biological effects on various cells and tissues. PAF-like activity has been detected in the spent media of two-cell to morula stage hamster embryos, leading to the suggestion that PAF may be the embryonic signal that hastens embryo transport to the uterus in this species. The present study was undertaken to examine whether the PAF receptor (PAFr) gene is expressed in hamster oviduct, and to identify the cell types in which the gene is expressed. DNA fragments complementary to the coding region of mRNA encoding hamster PAFr were cloned by reverse transcription-polymerase chain reaction (RT-PCR), identified by sequencing and used to prepare hamster specific cRNA probes. The presence of mRNA transcripts encoding the PAFr receptor in the oviduct was investigated by subjecting oviduct mRNA to RT-PCR. Southern blot analysis of the RT-PCR products verified the identity of the presumptive PAFr cDNAs. The cloned cDNA fragment of hamster PAFr was found to be highly conserved with respect to the receptor of other species, having 94.3% sequence similarity to the rat PAFr receptor. Hybridization histochemistry demonstrated that PAFr is expressed in the subepithelial cells and occasionally in the epithelium. In conclusion, expression of PAFr in the hamster oviduct is compatible with the proposed paracrine role of early embryo-derived PAF.
Velasquez
L A
LA
Unidad de Reproducción y Desarrollo, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
Ojeda
S R
SR
Croxatto
H B
HB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D017124:Conserved Sequence; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D005187:Fallopian Tubes / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled
D018076:DNA, Complementary; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor