Effect of medium composition on hybridoma growth and antibody production.
Two murine hybridoma cell lines named 1D12 and 3B1 secreting antibody against human blood group B were developed. Effect of medium composition and hyperosmotic stress on cell growth, viability and monoclonal antibody (MAb) production was examined during down-stream process in suspension culture. Both of the cell lines secreted MAb of higher titre if the basal medium was supplemented with 2.5 g/l L-glucose and 419 mg/l L-glutamine. Additional nutrients like transferrine, amino acids, ethanolamine, selenium were added to promote cell growth and viability. In case of cell line 1D12 enhancement of MAb production was observed using hyper-osmotic medium as well. Having data of these preliminary experiments we optimized the large scale production of MAbs in bioreactor. These MAbs serve as a base of the anti-B blood group reagent registered in Hungary.
Hencsey
Z
Z
HUMAN Co. Ltd. Gödölló, Hungary.
Fizil
A
A
Inzelt-Kovács
M
M
Veszely
G
G
Bánkúti
L
L
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002455:Cell Division; D002470:Cell Survival; D003470:Culture Media; D006801:Humans; D006825:Hybridomas; D051379:Mice; D014407:Tumor Cells, Cultured
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D003470:Culture Media