Multiplex amplification of STR loci with gender alleles using infrared fluorescence detection.
The analysis of short tandem repeat (STR) polymorphisms has proven extremely useful for gene mapping, paternity testing, and forensic analysis. Several commercial products are currently available for performing amplification and analysis of STRs. We have adapted Promega Geneprint Systems for use with a high sensitivity infrared (IR) fluorescent automated DNA sequencer. IR-labeled amplification products are generated by including a small quantity of IR-labeled dATP in the reaction. Several Geneprint STR loci can be multiplexed together with the amelogenin sex identification locus in a single amplification reaction. We have successfully amplified up to five Geneprint STR loci together with the amelogenin locus thus improving the throughput of analysis. Purified genomic DNA as well as simulated forensic samples have been utilized for these multiplex amplifications.
Steffens
D L
DL
LI-COR, Inc., Biotechnology Division, Lincoln, NE 68504, USA.
Roy
R
R
Brumbaugh
J A
JA
D000483:Alleles; D001331:Automation; D001826:Body Fluids / Q000737:chemistry; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis*; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D012732:Sex Determination Analysis / Q000379:methods*; D013055:Spectrophotometry, Infrared / Q000379:methods*
D005456:Fluorescent Dyes; D004247:DNA