Chemotherapy-induced expression of alpha B-crystallin in neuroblastoma.
Since alpha B-crystallin is known to be expressed in glial tissues of human brain and neuroectodermal tumors, the alpha B-crystallin content of neuroblastomas, may be related to the degree of glial or neuronal differentiation. The alpha B-crystallin content of 73 neuroblastomas, was determined by enzyme immunoassay. The concentration of alpha B-crystallin was examined in light of neuroblastoma prognostic factors. Neuroblastomas from patients who received chemotherapy (n = 23) contained higher concentrations of alpha B-crystallin than those from patients who did not receive chemotherapy (n = 50) (P > 0.05). There was a statistically significant difference in alpha B-crystallin concentrations in advanced stage patients who received preoperative chemotherapy (P < 0.01). Immunohistochemistry demonstrated alpha B-crystallin expression in the nerve-like fibers and a few ganglion-like cells. Staining was not apparent in the less differentiated cells in the tumor cell nest. alpha B-crystallin may play a role in the response to cellular stress in neuroblastoma.
Ishiguro
Y
Y
Department of Pediatric Surgery, Nagoya University School of Medicine, Japan.
Kato
K
K
Akatsuka
H
H
Iwata
H
H
Nagaya
M
M
D000293:Adolescent; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D003459:Crystallins / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009367:Neoplasm Staging; D009447:Neuroblastoma / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D016019:Survival Analysis
D003459:Crystallins; D006360:Heat-Shock Proteins