Monoclonal antibody raised against human mitotic cyclin B1, identifies cyclin B-like mitotic proteins in synchronized onion (Allium cepa L.) root meristem.
Cyclin B-like mitotic proteins have been detected in synchronized Allium cepa L. root tip cells by using mouse monoclonal anti-cyclin B1 antibody raised against human cyclin B1. Immunoblot shows two closely placed isoforms of cyclin B-like proteins having an apparent molecular weight around 54 kDa. In vivo [35S]-methionine labelling followed by immunoprecipitation and autoradiography indicates that cyclin B-like proteins are mainly synthesized in the G2 phase of the cell cycle and destroyed in late mitosis. Immunoblotting data depict that the level of cyclin B-like proteins reaches the maximum at the late G2 to early M phase; and it becomes degraded in the late hours of mitosis. Moreover, the cyclin B isoforms are stabilized in colchicine-arrested metaphase cells as already reported in animal cells.
Chaudhuri
S K
SK
Centre of Advanced Study in Botany, University of Calcutta, India.
Ghosh
S
S
D000490:Allium / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D015153:Blotting, Western; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D003429:Cross Reactions / Q000276:immunology; D019926:Cyclin B; D056744:Cyclin B1; D016213:Cyclins / Q000276:immunology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D016195:G2 Phase; D006651:Histocytochemistry; D006801:Humans; D018519:Meristem / Q000166:cytology / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008938:Mitosis; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; C534699:CCNB1 protein, human; C534700:Ccnb1 protein, mouse; D018797:Cell Cycle Proteins; D019926:Cyclin B; D056744:Cyclin B1; D016213:Cyclins; D010940:Plant Proteins; D008715:Methionine