title: Effect of glucose-6-phosphate dehydrogenase deficiency on neutrophil function.
abstract: The bactericidal activity of neutrophils depends primarily on free oxygen radicals released by the activation of NADPH oxidase when neutrophils are stimulated by microorganisms. Severe glucose-6-phosphate dehydrogenase (G6PD) deficiency is associated with decreased NADPH production. Increased susceptibility to recurrent bacterial infections in children with severe neutrophil G6PD deficiency as a consequence of decreased NADPH production has been reported earlier. In this study, the in vitro activity of neutrophils from normal and G6PD-deficient individuals was assessed by measuring the [14C]CO2 released via the hexose monophosphate shunt from radiolabeled [1-14C]-glucose and the nitroblue tetrazolium (NBT) dye reduction test. Our results show that the G6PD activity of neutrophils from 48 individuals, identified as severely erythrocyte (RBC) G6PD deficient (< 2 U/10(12) RBC) was 23% of the enzyme activity of neutrophils from 53 individuals with normal RBC G6PD levels (98.8 U/10(12) RBC). However, the results of functional assays of neutrophils as measured by hexose monophosphate shunt and the NBT test were comparable in G6PD-deficient and normal individuals, suggesting that a reduced activity of G6PD to as low as 23% of normal does not affect neutrophil function.
authors.affiliation: Arabian Gulf University College of Medicine and Medical Sciences, Manama, Bahrain.
authors.forename: K O
authors.initials: KO
authors.lastname: Ardati
authors.forename: K M
authors.initials: KM
authors.lastname: Bajakian
authors.forename: K S
authors.initials: KS
authors.lastname: Tabbara
mesh_terms: D000328:Adult; D002245:Carbon Dioxide / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology; D005947:Glucose / Q000378:metabolism; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood / Q000145:classification / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D009249:NADP / Q000096:biosynthesis / Q000172:deficiency; D009504:Neutrophils / Q000201:enzymology / Q000502:physiology*; D009580:Nitroblue Tetrazolium; D010427:Pentose Phosphate Pathway; D017382:Reactive Oxygen Species; D012720:Severity of Illness Index
chemical_list: D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D002245:Carbon Dioxide; D009580:Nitroblue Tetrazolium; D009249:NADP; D005947:Glucose