Detection of polymorphic FGF receptor 1 mRNA in bovine lens epithelium by PCR.
The expression of FGFR 1, 2 and 3 but not FGFR 4 was detected in bovine lens epithelial cells. FGFR 1 expression of both the 2-lg and 3-lg forms was detected by a difference in the lengths of the PCR products. Furthermore, mRNA which included or excluded six nucleotides corresponding to two amino acids in the acid box region of FGFR 1 was detected by a difference in mobility of the homo- and hetero-duplex strands formed in the PCR reaction.
Marunouchi
T
T
Division of Cell Biology, Fujita Health University, Aichi, Japan.
Hosoya
H
H
Kousaka
M
M
Lee
X R
XR
Majima
Y
Y
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002999:Clone Cells; D017931:DNA Primers; D004586:Electrophoresis; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D005822:Genetic Vectors; D007903:Lens Capsule, Crystalline / Q000737:chemistry* / Q000166:cytology; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D009692:Nucleic Acid Heteroduplexes; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1