title: Type III collagen messenger RNA is modulated in non-compliant human bladder tissue.
abstract: OBJECTIVE
  Previous studies have demonstrated that the non-compliant bladder is characterized histologically by an increased deposition of extracellular matrix protein, especially type III collagen, in the muscle wall. We sought to determine if an increased tissue level of type III collagen messenger RNA (mRNA) parallels the observed increase in protein expression.
  
  
  METHODS
  Using a reverse transcription-polymerase chain reaction (RT-PCR) quantitative technique we measured and compared the bladder tissue concentration of type III collagen mRNA between an experimental group of patients (n = 7) with urodynamically proven non-compliant bladders (< 12 cc/cm. H2O) and a control group (n = 8) with normal bladder compliance (> 20 cc/cm. H2O).
  
  
  RESULTS
  The mean (+/-S.E.M.) of type III collagen mRNA level in the non-compliant group was 11.72 +/- 2.56 attomole/milligram (amol/mg.) which was almost threefold higher than the level 4.26 +/- 0.74 amol/mg measured in the control group. This difference was statistically significant (p = 0.027).
  
  
  CONCLUSIONS
  Type III collagen mRNA levels are increased in the human non-compliant bladder. Therefore the accumulation of type III collagen protein is, in part, transcriptionally regulated.
authors.affiliation: Department of Urology, University of Texas Southwestern Medical Center, Dallas 75235-9110, USA.
authors.forename: E P
authors.initials: EP
authors.lastname: Kaplan
authors.forename: J C
authors.initials: JC
authors.lastname: Richier
authors.forename: P S
authors.initials: PS
authors.lastname: Howard
authors.forename: D H
authors.initials: DH
authors.lastname: Ewalt
authors.forename: V K
authors.initials: VK
authors.lastname: Lin
mesh_terms: D000293:Adolescent; D002648:Child; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D001750:Urinary Bladder, Neurogenic / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D003094:Collagen