Comparative study of ovomucoid isolated from Muscovy duck (Cairina moschata), domestic goose (Anser anser) and domestic duck (Anas platyrhynchos).
1. Ovomucoids were purified from Muscovy duck, domestic duck and domestic goose. 2. Peptide maps of cyanogen bromide-cleaved ovomucoids from Muscovy duck and domestic duck were very similar to one another, but differed from that of goose. 3. Muscovy duck ovomucoid showed the same protease inhibitory pattern as ovomucoid from domestic duck, inhibiting trypsin in the molar ratio of 1:2 and chymotrypsin 1:1. 4. Inhibitory complexes could be detected between chymotrypsin and ovomucoid from both Muscovy and domestic duck, but not from goose, by using non-denaturing gels. 5. No complexes could be detected between DFP-inactivated chymotrypsin and any of the ovomucoids. 6. The results show that of ovomucoid from Muscovy duck more closely resembles that from domestic duck than goose.
Nicol
K
K
Department of Biological and Molecular Sciences, University of Stirling, Scotland.
Watson
H
H
Stevens
L
L
D000818:Animals; D000829:Animals, Domestic; D002918:Chymotrypsin / Q000037:antagonists & inhibitors / Q000378:metabolism; D004372:Ducks / Q000145:classification / Q000378:metabolism*; D004529:Egg White / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary; D005260:Female; D005777:Geese / Q000145:classification / Q000378:metabolism*; D007531:Isoflurophate / Q000378:metabolism; D010059:Ovomucin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D010449:Peptide Mapping / Q000662:veterinary; D011480:Protease Inhibitors / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D007531:Isoflurophate; D010059:Ovomucin; D002918:Chymotrypsin