Expression of rac1 protein in the crypt-villus axis of rat small intestine: in reference to insulin action.
The expression of rho family along the crypt-villus axis of rat small intestine was investigated. The immunoblot analysis showed that the content of rac1 protein gradually increased from tip-villus to crypt-villus enterocytes without changes in the contents of rhoA and rac2 proteins. Furthermore, the contents of rac1 protein in the entire enterocytes were markedly decreased in streptozotocin-induced diabetic rats and restored by short-term treatment with insulin. These results suggest that differentiation and proliferation in the enterocytes of rat small intestine are possibly related to the expression of rac1 protein through insulin action.
Chono
E
E
Department of Physiological Chemistry, Hiroshima University School of Medicine, Minami-ku, Japan.
Kurokawa
T
T
Oda
C
C
Kawasaki
K
K
Yamamoto
T
T
Ishibashi
S
S
D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007328:Insulin / Q000502:physiology*; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020744:rac GTP-Binding Proteins
D007328:Insulin; D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins