Immunohistochemical localization of serotonin, galanin, cholecystokinin, and methionine-enkephalin in adrenal medullary cells of the chicken.
The identification of adrenaline- (A) and noradrenaline- (NA) containing cells in the adrenal medulla of the chicken and colocalization of serotonin and neuropeptides with A or NA in medullary cells were investigated with the use of immunohistochemical methods. Antisera against tyrosine hydroxylase and phenylethanolamine-N-methyltransferase were used as markers for catecholamine- and A-synthesizing cells, respectively. About 70% of catecholamine-synthesizing cells also exhibited immunoreactivity for phenylethanolamine-N-methyltransferase antiserum. Therefore, these cells are A-containing ones and the rest of cells seem to be NA-containing cells. Immunoreactivity with serotonin antiserum was observed in almost all medullary cells. Galanin-immunoreactivity was also found throughout the adrenal medulla, but was stronger in A-containing cells than in NA-containing ones. Cholecystokinin-immunoreactivity was restricted to A-containing cells. Methionine-enkephalin-immunoreactivity was seen in both A- and NA-containing cells, but in about half of medullary cells. From these results, it is suggested that serotonin, galanin, cholecystokinin, and methionine-enkephalin may be co-released with A and/or NA from adrenal medullary cells of the chicken.
Ohmori
Y
Y
Department of Veterinary Anatomy, School of Agriculture, Nagoya University, Japan.
Okada
Y
Y
Watanabe
T
T
D000313:Adrenal Medulla / Q000737:chemistry* / Q000166:cytology / Q000201:enzymology; D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D002766:Cholecystokinin / Q000032:analysis* / Q000276:immunology; D004744:Enkephalin, Methionine / Q000032:analysis* / Q000276:immunology; D004837:Epinephrine / Q000096:biosynthesis / Q000378:metabolism; D019004:Galanin / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D009638:Norepinephrine / Q000096:biosynthesis / Q000378:metabolism; D012701:Serotonin / Q000032:analysis* / Q000276:immunology
D012701:Serotonin; D004744:Enkephalin, Methionine; D019004:Galanin; D002766:Cholecystokinin; D009638:Norepinephrine; D004837:Epinephrine