Experimental model of obstructive jaundice in mongrel dog: study of subsequent pathological changes in liver.
BACKGROUND
Attempts at creating an experimental model of obstructive jaundice in large animals have been unsuccessful because of frequent fistulization of the ligated bile duct.


OBJECTIVE
To produce a model of obstructive jaundice in mongrel dogs and to study subsequent serial pathological changes in the liver.


METHODS
Four techniques were evaluated in four dogs to produce obstructive jaundice. After creation of a successful model, subsequent clinical and biochemical outcome and serial pathological changes in the liver were studied in another 14 dogs.


RESULTS
Complete extrahepatic biliary dissection produced a model of persistent obstructive jaundice. Serial histological changes included inflammation and edema in the first 14 days followed by lymphocytic infiltration at 28 days. Fibrosis started by day 7 and resulted in architectural distortion by day 21.


CONCLUSIONS
An acute, complete and irreversible model of obstructive jaundice can be produced by extrahepatic biliary tree excision and ligation. Serial pathological changes showed acute inflammation, followed by fibrosis and architectural distortion.
Kocher
H
H
Department of Gastroenterology, G S Medical College, Mumbai.
Bapat
R
R
Joshi
A
A
D000818:Animals; D002779:Cholestasis / Q000473:pathology*; D046508:Culture Techniques; D004195:Disease Models, Animal; D004285:Dogs; D004487:Edema / Q000473:pathology; D005260:Female; D007249:Inflammation / Q000473:pathology; D008099:Liver / Q000473:pathology*; D008111:Liver Function Tests; D008297:Male