title: Isolation and analysis of moenomycin and its biosynthetic intermediates from Streptomyces ghanaensis (ATCC 14672) wildtype and selected mutants.
abstract: Streptomyces ghanaensis (ATCC 14672) produces the phosphoglycolipid antibiotic moenomycin consisting of several components. A solid phase extraction procedure was developed which allowed a rapid isolation of both moenomycin and its biosynthetic intermediates from culture filtrates. Semi-preparative high performance liquid chromatography followed by high performance liquid chromatography-mass spectrometry provided structural data on the different moenomycin components. In order to obtain initial information on the biosynthetic pathway, moenomycin non-producing mutants were isolated. They were shown to release intermediates with shorter lipid chains suggesting that the lipid chain synthesis probably takes place at a later stage of the moenomycin biosynthesis. Based on the biological activity and the analytical data, we assume that a modification and in particular a shorter lipid portion drastically influences the inhibitory activity of this antibiotic.
authors.affiliation: Universität Bielefeld, Lehrstuhl für Genetik, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Subramaniam-Niehaus
authors.forename: T
authors.initials: T
authors.lastname: Schneider
authors.forename: J W
authors.initials: JW
authors.lastname: Metzger
authors.forename: W
authors.initials: W
authors.lastname: Wohlleben
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D001455:Bambermycins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016296:Mutagenesis; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D001455:Bambermycins