title: An electroneutral anion exchange mechanism is present in brush-border membranes isolated from eel kidney.
abstract: The mechanism of bicarbonate translocation across the luminal membrane of the eel (Anguilla anguilla) kidney tubular cells was studied by monitoring the uptake of H14CO3- into isolated brush-border membrane vesicles. Results indicate that the presence of a transmembrane outwardly directed Cl- gradient was able to transiently accumulate H14CO3- into the vesicular space, whereas neither an inwardly directed sodium gradient nor a transmembrane electrical potential gradient (inside positive) was able to stimulate the H14CO3- influx. This anion-dependent H14CO3- uptake was inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, suggesting that an anion exchanger was present in the brush-border membrane vesicles.
authors.affiliation: Laboratorio di Fisiologia Generale, Dipartimento di Biologia, Università di Lecce, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Vilella
authors.forename: L
authors.initials: L
authors.lastname: Ingrosso
authors.forename: V
authors.initials: V
authors.lastname: Zonno
authors.forename: T
authors.initials: T
authors.lastname: Schettino
authors.forename: C
authors.initials: C
authors.lastname: Storelli
mesh_terms: D000811:Anguilla; D000818:Animals; D000838:Anions / Q000494:pharmacology; D017920:Antiporters / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism*; D001692:Biological Transport; D002250:Carbon Radioisotopes; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides / Q000378:metabolism*; D007668:Kidney / Q000502:physiology*; D007700:Kinetics; D008564:Membrane Potentials; D008871:Microvilli / Q000502:physiology*; D009994:Osmolar Concentration
chemical_list: D000838:Anions; D017920:Antiporters; D001639:Bicarbonates; D002250:Carbon Radioisotopes; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides