Microplate assay for measurement of histamine release from mast cells.
A microplate-format assay for measurement of histamine release by activated rat peritoneal mast cells, with accompanying microplate-format spectrofluorometric assay of histamine, is demonstrated. Reproducibility and linearity of the histamine assay are comparable to the historical large format assay. Transfer of samples in the 96-well format, from histamine release through spectrofluorometric assay, greatly expedites handling and provides for better independence of each replicate. A sample experiment is included to show a typical size experiment easily done in this format.
Price
J A
JA
Oklahoma State University, College Osteopathic Medicine, Tulsa, USA. price@vms.ocom.okstate.edu
D000818:Animals; D011232:Chemical Precipitation; D005260:Female; D006632:Histamine / Q000032:analysis*; D006636:Histamine Release; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014238:Trichloroacetic Acid
D014238:Trichloroacetic Acid; D006632:Histamine