Cytokine-induced leukocyte rolling in mouse cremaster muscle arterioles in P-selectin dependent.
Leukocyte rolling and adhesion are generally observed in venules but rarely observed in arterioles. With the use of intravital microscopy, we found that a 4-h treatment with interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) dose dependently induced leukocyte rolling and adhesion in arterioles of the mouse cremaster muscle. The rolling response lasted more than 24 h and was completely inhibited by treatment with the sulfated polysaccharide fucoidin. Moreover, we found that costimulation with IL-1beta and TNF-alpha for 4 h synergistically increased arteriolar leukocyte rolling, i.e., threshold doses of IL-1beta and TNF-alpha together caused a more than 10-fold increase of rolling in arterioles compared with the sum of the individual responses. This rolling interaction was abolished by treatment with a monoclonal antibody directed against P-selectin (RB40.34), but it apparently was unaffected by a monoclonal antibody against L-selectin (MEL-14). Taken together, our functional data show that IL-1beta and TNF-alpha separately induce and synergistically increase P-selectin-dependent leukocyte rolling and firm adhesion in mouse cremaster arterioles.
Thorlacius
H
H
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Lindbom
L
L
Raud
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001160:Arterioles / Q000502:physiology*; D001161:Arteriosclerosis / Q000503:physiopathology; D002448:Cell Adhesion; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000098:blood supply*; D019007:P-Selectin / Q000276:immunology / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D019007:P-Selectin; D014409:Tumor Necrosis Factor-alpha