Different human tenascin-C variants in the extracellular matrix of cultured human fibroblasts.
Using an immunoadsorbent prepared with a monoclonal antibody specific for the high molecular mass isoform of human tenascin-C, we purified tenascin-C molecules containing at least one large subunit from the extracellular matrix of cultured normal human fibroblasts. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting analyses have shown that both high and low molecular mass subunits are present in these tenascin-C preparations. Because the monoclonal antibody used is able to bind only the high molecular mass isoform, the present data show that part of the tenascin-C present in the fibroblast extracellular matrix is made up of heterohexameric molecules.
Siri
A
A
Laboratory of Cell Biology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
Allemanni
G
G
Gaggero
B
B
Zardi
L
L
D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000737:chemistry*; D006801:Humans; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D019063:Tenascin / Q000032:analysis* / Q000737:chemistry
D046911:Macromolecular Substances; D019063:Tenascin