Effects of mitomycin C on the expression of proliferating cell nuclear antigen after filtering surgery in rabbits.
BACKGROUND
The aim of the study was to evaluate the effects of mitomycin C on the cell replication activity in wound healing following experimental filtration surgery.


METHODS
Trabeculectomy with or without application of mitomycin C was performed on albino rabbit eyes. At 1, 4, 7, 14, and 28 days after surgery, the expression of proliferating cell nuclear antigen, a marker of cell proliferation, in the filtering site was examined immunohistochemically using a streptavidin-biotin complex method.


RESULTS
In control eyes that underwent trabeculectomy but did not receive mitomycin C, the number of immunoreactive cells increased 4-7 days after operation and decreased markedly at around 14 days. The filtering site was obstructed histologically at 4-7 days after operation. In the mitomycin C-treated eyes, immunoreactive cells appeared 4 days after surgery but disappeared by 7 days at the surgical site. The number of immunoreactive cells in the treated eyes was much lower than that in control eyes.


CONCLUSIONS
The cell replication activity was markedly inhibited by administration of mitomycin C. The filtering site remained open for 28 days after surgery, whereas it was completely obstructed within 7 days in control eyes. Immunocytochemistry for proliferating cell nuclear antigen, as used in this study, is a simple and reliable method for detection of cell replication activity.
Watanabe
J
J
Department of Ophthalmology, Niigata University, School of Medicine, Japan.
Sawaguchi
S
S
Fukuchi
T
T
Abe
H
H
Zhou
L
L
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D015415:Biomarkers; D002455:Cell Division; D017024:Chemotherapy, Adjuvant; D003315:Cornea / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D004195:Disease Models, Animal; D005500:Follow-Up Studies; D007150:Immunohistochemistry; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis* / Q000187:drug effects; D011817:Rabbits; D012590:Sclera / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D014130:Trabeculectomy; D014945:Wound Healing / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen; D016685:Mitomycin