High-performance liquid chromatography/electrospray mass spectrometry for the analysis of modified bases in DNA: 7-(2-hydroxyethyl)guanine, the major ethylene oxide-DNA adduct.
A method was developed for the analysis of 7-(2-hydroxyethyl)guanine (7HEG), the major DNA adduct formed after exposure to ethylene oxide (EO). The method is based on DNA neutral thermal hydrolysis, adduct micro-concentration, and final characterization and quantification by HPLC coupled to single-ion monitoring electrospray mass spectrometry (HPLC/SIR-ESMS). The method was found to be selective, sensitive, and easy to handle with no need for enzymatic digestion or previous sample derivatization. Detection limit was found to be close to 1 fmol of adduct injected (10(-10) M), thus allowing the detection of approximately three modified bases on 10(8) intact nucleotides in blood sample analysis. Quantification results are shown for 7HEG after calf thymus DNA and blood exposure to various doses of EO, in both cases obtaining clear dose-response relationships.
Leclercq
L
L
Laboratoire d'Oncologie, Radiologie et Mutagenèse Expérimentales (ORME), CHU B23, Liege, Belgium.
Laurent
C
C
De Pauw
E
E
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000187:drug effects* / Q000378:metabolism*; D018736:DNA Adducts / Q000032:analysis*; D005027:Ethylene Oxide / Q000378:metabolism* / Q000494:pharmacology*; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods
D018736:DNA Adducts; C035018:N(7)-hydroxyethylguanine; D006147:Guanine; D004247:DNA; D005027:Ethylene Oxide