Evidence for and characterization of cytochrome P-450 in Neurospora crassa.
Cytochrome P-450 was detected in microsomes and presumably in cytosol of Neurospora crassa, and was found to be inducible by progesterone. In the microsomal fraction cytochrome b5 and NADPH-cytochrome c reductase activities were measurable, too. Cytochrome P-450 of Neurospora crassa is inhibited by SKF-525 A and by inhibitors of ergosterol biosynthesis. After induction of cytochrome P-450 with progesterone 11 alpha-hydroxyprogesterone as one metabolite of progesterone was detected in the culture media as well as in the mycelia. After 42 hours about 70% of progesterone were metabolized.
Nega
E
E
Grunwaldt
G
G
D000935:Antifungal Agents / Q000494:pharmacology; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D015786:Cytochromes b5 / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015725:Fluconazole / Q000494:pharmacology; D005659:Fungicides, Industrial / Q000494:pharmacology; D008861:Microsomes / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009492:Neurospora crassa / Q000378:metabolism*; D011335:Proadifen / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
D000935:Antifungal Agents; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D005659:Fungicides, Industrial; D014230:Triazoles; C087058:penconazole; D011374:Progesterone; D015725:Fluconazole; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D011335:Proadifen; D009251:NADPH-Ferrihemoprotein Reductase