title: Evidence for the formation of an unusual ternary complex of rabbit liver EF-1alpha with GDP and deacylated tRNA.
abstract: Eukaryotic translation elongation factor 1alpha is known to interact in GTP-bound form with aminoacyl-tRNA promoting its binding to the ribosome. In this paper another ternary complex [EF-1alpha*GDP*deacylated tRNA], never considered in widely accepted elongation schemes, is reported for the first time. The formation of this unusual complex, postulated earlier (FEBS Lett. (1996) 382, 18-20), has been detected by four independent methods. [EF-1alpha*GDP]-interacting sites are located in the acceptor stem, TpsiC stem and TpsiC loop of tRNA(Phe) and tRNA(Leu) molecules. Both tRNA and EF-1alpha are found to undergo certain conformational changes during their interaction. The ability of EF-1alpha to form a complex with deacylated tRNA indicates that the factor may perform an important role in tRNA and aminoacyl-tRNA channeling in higher eukaryotic cells.
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Petrushenko
authors.forename: B S
authors.initials: BS
authors.lastname: Negrutskii
authors.forename: A S
authors.initials: AS
authors.lastname: Ladokhin
authors.forename: T V
authors.initials: TV
authors.lastname: Budkevich
authors.forename: V F
authors.initials: VF
authors.lastname: Shalak
authors.forename: A V
authors.initials: AV
authors.lastname: El'skaya
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005453:Fluorescence; D006153:Guanosine Diphosphate / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000378:metabolism*; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism*; D012356:RNA, Transfer, Leu / Q000378:metabolism; D012360:RNA, Transfer, Phe / Q000378:metabolism; D011817:Rabbits; D012261:Ribonucleoproteins / Q000378:metabolism*; D014364:Tryptophan / Q000737:chemistry
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012356:RNA, Transfer, Leu; D012360:RNA, Transfer, Phe; D012261:Ribonucleoproteins; D006153:Guanosine Diphosphate; D014364:Tryptophan; D012343:RNA, Transfer