Stereospecific oxidation of (R)- and (S)-1-indanol by naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4.
A recombinant Escherichia coli strain which expresses naphthalene dioxygenase (NDO) from Pseudomonas sp. strain NCIB 9816-4 oxidized (S)-1-indanol to trans-(1S,3S)-indan-1,3-diol (95.5%) and (R)-3-hydroxy-1-indanone (4.5%). The same cells oxidized (R)-1-indanol to cis-1,3-indandiol (71%), (R)-3-hydroxy-1-indanone (18.2%), and cis-1,2,3-indantriol (10.8%). Purified NDO oxidized (S)-1-indenol to both syn- and anti-2,3-dihydroxy-1-indanol.
Lee
K
K
Department of Microbiology, University of Iowa, Iowa City 52242, USA.
Resnick
S M
SM
Gibson
D T
DT
D049308:Dioxygenases; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D007189:Indans / Q000737:chemistry / Q000378:metabolism*; D007536:Isomerism; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism*; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism; D011995:Recombination, Genetic
D007189:Indans; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; C016515:1-indanol; D010105:Oxygenases; D049308:Dioxygenases; C038703:naphthalene dioxygenase