Glucose transport correlates with GLUT2 abundance in rat liver during altered thyroid status.
Glucose transport activity ([3H]D-glucose uptake) in liver sinusoidal membrane vesicles (SMVs) from hyperthyroid rats was significantly higher than that from euthyroid controls (2.1-times increase in V(max) with K(m) unchanged at approximately 18 mM), associated with increased GLUT2 expression. In contrast, glucose transport V(max) into SMVs from hypothyroid rats was reduced to 0.75-times that of euthyroid controls, associated with a reduced GLUT2 abundance. GLUT1 expression in SMVs was unaffected by changes in thyroid status. GLUT2, but not GLUT1 abundance on the blood-facing membrane of liver cells is sensitive to changes in thyroid status and these changes in transporter expression directly correlate (r = 0.96) with altered glucose transport activity.
Kemp
H F
HF
Department of Anatomy and Physiology, University of Dundee, UK.
Hundal
H S
HS
Taylor
P M
PM
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D051273:Glucose Transporter Type 2; D006980:Hyperthyroidism / Q000378:metabolism*; D007037:Hypothyroidism / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013961:Thyroid Gland / Q000502:physiology*
D051273:Glucose Transporter Type 2; D009004:Monosaccharide Transport Proteins; D005947:Glucose