A probable conformational difference between recombinant and urinary erythropoietins.
Urinary and recombinant human erythropoietin differ with respect to ease of iodination, inactivation by iodination, second derivative and circular dichroic spectra, rate of inactivation by trypsin and glycosylation pattern. All of these differences are compatible with a significant difference in conformation of these two forms of erythropoietin.
Kung
C K
CK
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA.
Goldwasser
E
E
D000818:Animals; D002942:Circular Dichroism; D004921:Erythropoietin / Q000737:chemistry* / Q000235:genetics / Q000652:urine*; D006801:Humans; D006868:Hydrolysis; D007457:Iodine Radioisotopes / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin / Q000737:chemistry
D007457:Iodine Radioisotopes; D011994:Recombinant Proteins; D004921:Erythropoietin; D014357:Trypsin