title: The enzymatic activity of Cu/Zn superoxide dismutase does not fluctuate in mouse spermatogenic cells despite mRNA changes.
abstract: In the mammalian testis, multiple mRNAs encoding the copper zinc superoxide dismutase (SOD-1) are expressed in postmeiotic male germ cells. Here we relate SOD-1 mRNA levels to SOD-1 protein and enzyme activity levels in mouse spermatogenic cells. Although the sizes and relative amounts of the multiple SOD-1 mRNAs vary as male germ cells enter meiosis and proceed into the postmeiotic stages of spermatogenesis, the amount of SOD-1 protein and enzyme activity does not fluctuate significantly, suggesting a precise control of SOD-1 activity in male germ cells.
authors.affiliation: Department of Biology, Tufts University, Medford, Massachusetts 02155, USA.
authors.forename: W
authors.initials: W
authors.lastname: Gu
authors.forename: N B
authors.initials: NB
authors.lastname: Hecht
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004790:Enzyme Induction; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008540:Meiosis; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism*; D013087:Spermatids / Q000201:enzymology*; D013090:Spermatocytes / Q000201:enzymology*; D013091:Spermatogenesis; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D013482:Superoxide Dismutase