Elevated levels of ERK2 in human breast carcinoma MCF-7 cells transfected with protein kinase C alpha.
We investigated the effect of elevated levels of protein kinase C alpha (PKC alpha) on cell proliferation in human breast carcinoma cells (MCF-7). MCF-7 cells transfected with either the pSV2M(2)6 vector without the insert (MCF-7/Vector) or containing a full length cDNA encoding PKC alpha (MCF-7/PKC alpha) were compared. MCF-7/PKC alpha cells were found to have an increased proliferative rate with a doubling time of 15 h as compared to 42 h for MCF-7/Vector cells. Flow cytometry illustrated a greater percentage of MCF-7/PKC alpha cells in the S phase of the cell cycle. Western and Northern blot analyses demonstrated an increase in extracellular regulated protein kinase 2 (ERK2) gene expression in MCF-7/PKC alpha cells but no alteration of this gene expression in MCF-7/Vector cells. These results suggested that the elevated level of ERK2 which is also known as mitogen activated protein kinase is probably involved in the increase in MCF-7/PKC alpha cell proliferation.
Gupta
A K
AK
Department of Radiation Oncology, William Beaumont Hospital, Michigan, Royal Oak 48073, USA.
Galoforo
S S
SS
Berns
C M
CM
Martinez
A A
AA
Corry
P M
PM
Guan
K L
KL
Lee
Y J
YJ
D015152:Blotting, Northern; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis*; D002455:Cell Division / Q000235:genetics*; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D051571:Protein Kinase C-alpha; D016196:S Phase; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism
D018076:DNA, Complementary; D007527:Isoenzymes; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1