[The study of the in vitro effect of selenium on formation of active oxygen species and lipid peroxidation in rat liver microsomes].
In vitro influence of sodium selenite on cytochrome P-450-dependent formation of active oxygen species on lipid peroxidation (LPO) in rat liver microsomes was studied. Sodium selenite (10(-6)-10(-3) M) did not influence rates of NADPH/Fe-induced formation of active oxygen species (O2.-, OH., H2O2) and NAPDH-dependent LPO. Only at 10(-3) M selenite caused significant decrease in production of hydrogen peroxide in microsomes. Data obtained suggest that sodium selenite at physiological concentrations does not influence formation of active oxygen species by cytochrome P-450 and the rate of enzymatic LPO in rat liver microsomes.
Lashneva
N V
NV
Karge
E
E
Knells
U
U
Klinger
V
V
Splinter
F K
FK
Tutel'ian
V A
VA
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D018038:Sodium Selenite / Q000494:pharmacology*
D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D006861:Hydrogen Peroxide; D018038:Sodium Selenite
Izuchenie vliianiia selena in vitro na obrazovanie aktivnykh form kisloroda i perekisnoe okislenie lipidov v mikrosomakh pecheni krys.