title: A newly identified member of the tumor necrosis factor receptor superfamily with a wide tissue distribution and involvement in lymphocyte activation.
abstract: The tumor necrosis factor receptor (TNFR) superfamily consists of approximately 10 characterized members of human proteins. We have identified a new member of the TNFR superfamily, TR2, from a search of an expressed sequence tag data base. cDNA cloning and Northern blot hybridization demonstrated multiple mRNA species, of which a 1.7-kilobase form was most abundant. However, TR2 is encoded by a single gene which, maps to chromosome 1p36.22-36.3, in the same region as several other members of the TNFR superfamily. The most abundant TR2 open reading frame encodes a 283-amino acid single transmembrane protein with a 36-residue signal sequence, two perfect and two imperfect TNFR-like cysteine-rich domains, and a short cytoplasmic tail with some similarity to 4-1BB and CD40. TR2 mRNA is expressed in multiple human tissues and cell lines and shows a constitutive and relatively high expression in peripheral blood T cells, B cells, and monocytes. A TR2-Fc fusion protein inhibited a mixed lymphocyte reaction-mediated proliferation suggesting that the receptor and/or its ligand play a role in T cell stimulation.
authors.affiliation: Department of Microbiology and Immunology and Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA. kkwon@sunflower.bio.indiana.edu
authors.forename: B S
authors.initials: BS
authors.lastname: Kwon
authors.forename: K B
authors.initials: KB
authors.lastname: Tan
authors.forename: J
authors.initials: J
authors.lastname: Ni
authors.forename: K O
authors.initials: KO
authors.lastname: Oh
authors.forename: Z H
authors.initials: ZH
authors.lastname: Lee
authors.forename: K K
authors.initials: KK
authors.lastname: Kim
authors.forename: Y J
authors.initials: YJ
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Gentz
authors.forename: G L
authors.initials: GL
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Harrop
authors.forename: S D
authors.initials: SD
authors.lastname: Lyn
authors.forename: C
authors.initials: C
authors.lastname: Silverman
authors.forename: T G
authors.initials: TG
authors.lastname: Porter
authors.forename: A
authors.initials: A
authors.lastname: Truneh
authors.forename: P R
authors.initials: PR
authors.lastname: Young
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008969:Molecular Sequence Data; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; D014018:Tissue Distribution
chemical_list: D018124:Receptors, Tumor Necrosis Factor; D053240:Receptors, Tumor Necrosis Factor, Member 14; D011991:Receptors, Virus; C506028:TNFRSF14 protein, human