title: Filovirus-like particles detected in the leafhopper Psammotettix alienus.
abstract: Filamentous particles were detected by negative contrast electron microscopy of extracts from the leafhopper Psammotettix alienus (Dahlbom) reared on healthy Festuca gigantea plants. The particles were straight, slightly curved or flexuous, sometimes with the one end curled into a ring with an outer diameter of about 200 nm. Length distribution of 280 particles showed a minor and a major group with median length of about 600 and 1,100 nm, respectively. Projections, 8-10 nm long and about 10 nm apart, were evenly distributed on the surface. The diameter of particles, including projections, was 55-70 nm. Partly disintegrated revealed an internal structure about 30 nm in diameter and with cross-striation with a periodicity of 5-5.5 nm. In some particles, a central canal, 5-10 nm in diameter, could be seen at one end. Ultramicrotomy of leafhopper heads showed that some cells contained intracytoplasmic clusters of particles together with filamentous structures. The particles described in this paper resemble virions in the virus family Filoviridae, but can be distinguished by having a smaller particle diameter. The name Taastrup virus is suggested for the putative virus from Psammotettix alienus, according to the place it was first detected.
authors.affiliation: Department of Plant Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark. tl@kvl.dk
authors.forename: T
authors.initials: T
authors.lastname: Lundsgaard
mesh_terms: D000818:Animals; D016563:Filoviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D007313:Insecta / Q000821:virology*; D010936:Plant Extracts; D014771:Virion
chemical_list: D010936:Plant Extracts