title: The PerFect lipid optimizer kit for maximizing lipid-mediated transfection of eukaryotic cells.
abstract: The transfection efficiencies of a panel of eight uniquely different lipid reagents has been evaluated with two other commercially available lipids for use in transfecting a diversity of eukaryotic cell lines. The PerFect lipids are available individually or together in an optimization panel format that can be tested in any given cell line, enabling one to evaluate the optimal lipid for transfecting each individual cell line. Our results demonstrate that no single lipid is optimal for plasmid transfection over a broad range of cell types, thus emphasizing the need for multiple unique lipid reagents and a simple format for testing their transfection efficiency on a given cell type.
authors.affiliation: Invitrogen Corporation, San Diego, CA, USA.
authors.forename: T
authors.initials: T
authors.lastname: Griffiths
authors.forename: M
authors.initials: M
authors.lastname: Russell
authors.forename: K
authors.initials: K
authors.lastname: Froning
authors.forename: B D
authors.initials: BD
authors.lastname: Brown
authors.forename: S M
authors.initials: SM
authors.lastname: Scanlon
authors.forename: M
authors.initials: M
authors.lastname: Almazan
authors.forename: R
authors.initials: R
authors.lastname: Marcil
authors.forename: J P
authors.initials: JP
authors.lastname: Hoeffler
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001943:Breast Neoplasms; D016466:CHO Cells; D019556:COS Cells; D002460:Cell Line; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids; D051379:Mice; D009474:Neurons; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D007202:Indicators and Reagents; D008055:Lipids; D001616:beta-Galactosidase