title: Actions and interactions of growth hormone and insulin-like growth factor-II: body and organ growth of transgenic mice.
abstract: To characterize long-term actions and interactions of growth hormone (GH) and insulin-like growth factor-II (IGF-II) on postnatal body and organ growth, hemizygous phosphoenolpyruvate carboxykinase (PEPCK)-human IGF-II transgenic mice were crossed with hemizygous PEPCK-bovine GH transgenic mice. The latter are characterized by two-fold increased serum levels of IGF-I and exhibit markedly increased body, skeletal and organ growth. Four different genetic groups were obtained: mice harbouring the IGF-II transgene (I), the bGH transgene (B), or both transgenes (IB), and non-transgenic controls (C). These groups of mice have previously been studied for circulating IGF-I levels (Wolf et al., 1995a), whereas the present study deals with body and organ growth. Growth curves (week 3 to 12) were estimated by regression with linear and quadratic components of age on body weight and exhibited significantly (p < 0.001) greater linear coefficients in B and IB than in I and C mice. The linear coefficients of male I and C mice were significantly (p < 0.001) greater than those of their female counterparts, whereas this sex-related difference was absent in the bGH transgenic groups. The weights of internal organs as well as the weights of abdominal fat, skin and carcass were recorded from 3.5- to 8-month-old mice. In addition, organ weight-to-body weight-ratios (relative organ weights) were calculated. Except for the weight of abdominal fat, absolute organ weights were as a rule significantly greater in B and IB than in I and C mice. IGF-II overproduction as a tendency increased the weights of kidneys, adrenal glands, pancreas and uterus both in the absence and presence of the bGH transgene. Analysis of relative organ weights demonstrated significant (p < 0.05) effects of elevated IGF-II on the relative growth of kidneys (males and females) and adrenal glands (females), confirming our previous report on organ growth of PEPCK-IGF-II transgenic mice. In females, IGF-II and GH overproduction were additive in stimulating the growth of spleen and uterus, providing evidence for tissue-specific postnatal growth promoting effects by IGF-II in the presence of elevated IGF-I.
authors.affiliation: Lehrstuhl für Molekulare Tierzucht und Haustiergenetik, Ludwig-Maximilians-Universität, München, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Blackburn
authors.forename: A
authors.initials: A
authors.lastname: Schmitt
authors.forename: P
authors.initials: P
authors.lastname: Schmidt
authors.forename: R
authors.initials: R
authors.lastname: Wanke
authors.forename: W
authors.initials: W
authors.lastname: Hermanns
authors.forename: G
authors.initials: G
authors.lastname: Brem
authors.forename: E
authors.initials: E
authors.lastname: Wolf
mesh_terms: D000005:Abdomen / Q000254:growth & development; D000273:Adipose Tissue / Q000502:physiology; D000818:Animals; D001835:Body Weight / Q000235:genetics; D005260:Female; D013006:Growth Hormone / Q000235:genetics* / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000254:growth & development; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000254:growth & development*; D009929:Organ Size / Q000235:genetics*; D012867:Skin / Q000254:growth & development
chemical_list: D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D013006:Growth Hormone