title: Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination.
abstract: Novel approaches for the generation of more effective vaccines for HIV-1 are of significant importance. In this report we analyze the immunogenicity and efficacy of an HIV-1 DNA vaccine encoding env, rev and gag/pol in a chimpanzee model system. The immunized animals developed specific cellular and humoral immune responses. Animals were challenged with a heterologous chimpanzee titered stock of HIV-1 SF2 virus and followed for 48 weeks after challenge. Polymerase chain reaction coupled with reverse transcription (RT-PCR) results indicated infection in the control animal, whereas those animals vaccinated with the DNA constructs were protected from the establishment of infection. These studies serve as an important benchmark for the use of DNA vaccine technology for the production of protective immune responses.
authors.affiliation: Department of Pathology, University of Pennsylvania, Philadelphia 19104, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Boyer
authors.forename: K E
authors.initials: KE
authors.lastname: Ugen
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Agadjanyan
authors.forename: L
authors.initials: L
authors.lastname: Gilbert
authors.forename: M L
authors.initials: ML
authors.lastname: Bagarazzi
authors.forename: M
authors.initials: M
authors.lastname: Chattergoon
authors.forename: P
authors.initials: P
authors.lastname: Frost
authors.forename: A
authors.initials: A
authors.lastname: Javadian
authors.forename: W V
authors.initials: WV
authors.lastname: Williams
authors.forename: Y
authors.initials: Y
authors.lastname: Refaeli
authors.forename: R B
authors.initials: RB
authors.lastname: Ciccarelli
authors.forename: D
authors.initials: D
authors.lastname: McCallus
authors.forename: L
authors.initials: L
authors.lastname: Coney
authors.forename: D B
authors.initials: DB
authors.lastname: Weiner
mesh_terms: D016915:AIDS Vaccines / Q000627:therapeutic use*; D000818:Animals; D018106:CD28 Antigens / Q000097:blood; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control*; D015497:HIV-1 / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008198:Lymph Nodes / Q000821:virology; D008297:Male; D009500:Neutralization Tests; D002679:Pan troglodytes; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000627:therapeutic use*; D019562:Viral Load
chemical_list: D016915:AIDS Vaccines; D018106:CD28 Antigens; D004279:DNA, Viral; D015483:HIV Antibodies; D019444:Vaccines, DNA