title: Biochemical and phylogenetic characterization of two novel deep-sea Thermococcus isolates with potentially biotechnological applications.
abstract: The partial 16S rDNA gene sequences of two thermophilic archaeal strains, TY and TYS, previously isolated from the Guaymas Basin hydrothermal vent site were determined. Lipid analyses and a comparative analysis performed with 16S rDNA sequences of similar thermophilic species showed that the strains isolated from deep-sea vents were not identical to the other species belonging to the genus Thermococcus. On the basis of the results of the phylogenetic analyses, lipid analyses, and previously reported physiological data, we believe that strains TY and TYS are significantly different from the previously described Thermococcus species. According to specific physiological and molecular features, we propose the use of these isolates as potential tools for the development of biotechnological applications in the field of starch processing and DNA technology.
authors.affiliation: Department of Agrobiology and Agrochemistry, University of Tuscia, via C. de Lellis, I-01100 Viterbo, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Canganella
authors.forename: W J
authors.initials: WJ
authors.lastname: Jones
authors.forename: A
authors.initials: A
authors.lastname: Gambacorta
authors.forename: G
authors.initials: G
authors.lastname: Antranikian
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D008055:Lipids / Q000032:analysis; D010802:Phylogeny; D014871:Water Microbiology
chemical_list: D008055:Lipids