MALDI-MS as a monitor of the purification and folding of synthetic eclosion hormone.
Analogues of the small protein Manduca sexta eclosion hormone (62 amino acids) were synthesized by Fmoc solid-phase methodology. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) was used to analyze the products of the syntheses and this information was used to design an efficient purification scheme. MALDI-MS was used to monitor the target products through purification and it was also used to monitor folding of the purified materials. The folded EH analogues were shown to be biologically active proteins with an in vivo bioassay using pharate adult moths, Heliothis virescens.
Wang
Y J
YJ
Department of Entomology, Texas A&M University, College Station 77843, USA.
Yurttas
L
L
Dale
B E
BE
Russell
D H
DH
Kinsel
G
G
Preston-Schaffter
L M
LM
Johnson
V
V
Hayes
T K
TK
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D007301:Insect Hormones / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D018402:Manduca; D008969:Molecular Sequence Data; D017510:Protein Folding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013329:Structure-Activity Relationship
D007301:Insect Hormones; C042645:eclosion hormone