Cell-mediated immune responses of lambs to experimental infection with bovine respiratory syncytial virus.
Primary infection of lambs with bovine respiratory syncytial virus (RSV) was characterised by the presence of virus-specific cytotoxic cells in the peripheral blood as early as 5 days post-infection. These effector cells lysed virus-infected autologous targets in a self-restricted manner. Depletion techniques revealed that cytotoxic activity was largely due to OvCD8+ cells. Neutrophils obtained from experimentally infected lambs 5 to 10 days post-inoculation exhibited significant cytotoxic activity in the presence of bovine RSV-specific antiserum. During the same period there was a significant lymphoproliferative response to live or inactivated bovine RSV. Lymphoproliferative and cytotoxic activity coincided with the clearance of the virus from nasal secretions.
Sharma
A K
AK
University of Liverpool, Department of Veterinary Pathology, Veterinary Field Station, UK.
Woldehiwet
Z
Z
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002417:Cattle; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008297:Male; D009500:Neutralization Tests; D009504:Neutrophils / Q000276:immunology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology* / Q000302:isolation & purification; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000821:virology
D000914:Antibodies, Viral