title: Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon.
abstract: The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.
authors.affiliation: Centre d'Ingénierie des Protéines, Université de Liège, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Brans
authors.forename: A
authors.initials: A
authors.lastname: Loriaux
authors.forename: I
authors.initials: I
authors.lastname: Thamm
authors.forename: B
authors.initials: B
authors.lastname: Joris
authors.forename: J
authors.initials: J
authors.lastname: Dusart
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D006360:Heat-Shock Proteins; D011994:Recombinant Proteins