Rapid reconstitution of tonoplast H(+)-translocating pyrophosphatase from cultured rice cells into liposomes.
We report the rapid and functional reconstitution of H(+)-pyrophosphatase (H(+)-PPase) from the tonoplast of cultured rice (Oryza sativa L.) cells to proteoliposomes. The CHAPS-solubilized H(+)-PPase was incorporated into liposomes by gel-filtration. Both the activities of PPi-hydrolysis and H(+)-pumping were influenced by the lipid-protein ratio and cholesterol.
Nakamura
Y
Y
Molecular Function Laboratory, National Food Research Institute, Ibaraki, Japan.
Sakakibara
Y
Y
Kobayashi
H
H
Kasamo
K
K
D015153:Blotting, Western; D002784:Cholesterol / Q000494:pharmacology; D002850:Chromatography, Gel; D043564:Inorganic Pyrophosphatase; D007700:Kinetics; D008081:Liposomes; D012275:Oryza / Q000201:enzymology*; D011510:Proteolipids / Q000378:metabolism*; D011755:Pyrophosphatases / Q000302:isolation & purification / Q000378:metabolism*
D008081:Liposomes; D011510:Proteolipids; C020485:proteoliposomes; D002784:Cholesterol; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase