title: Emergence of the trimethoprim resistance gene dfrD in Listeria monocytogenes BM4293.
abstract: The sequence of the trimethoprim resistance gene of the 3.7-kb plasmid (pIP823) that confers high-level resistance (MIC, 1,024 microg/ml) to Listeria monocytogenes BM4293 was determined. The gene was identical to dfrD recently detected in Staphylococcus haemolyticus MUR313. The corresponding protein, S2DHFR, represents the second class of high-level trimethoprim-resistant dihydrofolate reductase identified in gram-positive bacteria. We propose that trimethoprim resistance in L. monocytogenes BM4293 could originate in staphylococci.
authors.affiliation: Unité des Agents Antibactériens, Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Charpentier
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D000595:Amino Acid Sequence; D008089:Listeria monocytogenes / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D014296:Trimethoprim Resistance / Q000235:genetics*