Expression of receptors for gut peptides in human pancreatic adenocarcinoma and tumour-free pancreas.
Gut hormones that modulate the growth of normal pancreas may also modulate the growth of cancers originating from pancreas. This study visualized and compared the receptors for cholecystokinin (CCK), bombesin (BBS), secretin and vasoactive intestinal peptide (VIP) in tumour-free tissue sections of human pancreas (n = 10) and pancreatic ductal adenocarcinomas (n = 12) with storage phosphor autoradiography using radioligands. CCK-B receptors, present in control pancreata, were not detected in any of the pancreatic cancers. BBS receptors were visualized in control pancreata, but they were absent in 10 of 12 pancreatic cancers. In 5 of 12 pancreatic cancers, receptors for secretin were visualized, while binding for secretin was present in all tumour-free pancreata. Conversely, no specific binding of VIP was detected in control pancreata but was identified in 3 of 12 pancreatic cancer specimens. It is concluded that the expression of gut peptide receptors in pancreatic cancer differs from that in tumour-free pancreas. Receptors for these peptides are present in only a minority of pancreatic cancer specimens.
Tang
C
C
Department of Gastroenterology, University Hospital Leiden, The Netherlands.
Biemond
I
I
Offerhaus
G J
GJ
Verspaget
W
W
Lamers
C B
CB
D000230:Adenocarcinoma / Q000648:ultrastructure*; D000328:Adult; D000368:Aged; D001345:Autoradiography; D001839:Bombesin / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000648:ultrastructure*; D010190:Pancreatic Neoplasms / Q000648:ultrastructure*; D018004:Receptors, Bombesin / Q000378:metabolism*; D011949:Receptors, Cholecystokinin / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism*; D018005:Receptors, Vasoactive Intestinal Peptide / Q000378:metabolism*; D012633:Secretin / Q000378:metabolism; D012680:Sensitivity and Specificity; D012844:Sincalide / Q000031:analogs & derivatives / Q000378:metabolism; D013388:Succinimides / Q000378:metabolism
C044758:Bolton Hunter-cholecystokinin octapeptide; D007457:Iodine Radioisotopes; D018004:Receptors, Bombesin; D011949:Receptors, Cholecystokinin; D043562:Receptors, G-Protein-Coupled; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D013388:Succinimides; C029937:secretin receptor; D012633:Secretin; C035891:bombesin, Tyr(4)-; D012844:Sincalide; D001839:Bombesin