title: Somatostatin does not affect multiplication of granule cells in the rat cerebellum.
abstract: Somatostatin receptor are transiently expressed by immature granule cells of rat cerebellum. The effects of somatostatin and octreotide on cell proliferation were studied in cultured cerebellar explants from 10-day-old rats. Cell multiplication was measured using [3H]thymidine incorporation and flow cytometric analysis of cell cycle parameters. [3H]Thymidine incorporation occurred exclusively in neuroblasts of the external granule cell layer in the presence of insulin (1 microM). The labeling index, the length of the S phase, and the potential doubling time were similar in vivo and in explants. Octreotide (10(-12) to 10(-6)M) had no effect on [3H]thymidine incorporation in cerebellar explants. In addition, somatostatin and octreotide did not modify the proportion of cells in the S, G0-G1, and G2-M phases. The present results demonstrate that somatostatin does not affect cell multiplication in neurons of the external granule cell layer.
authors.affiliation: European Institute for Peptide Research (IFRMP No. 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, Mont-Saint-Aignan, France.
authors.forename: C
authors.initials: C
authors.lastname: Bodenant
authors.forename: A
authors.initials: A
authors.lastname: Laquerrière
authors.forename: M
authors.initials: M
authors.lastname: Paresy
authors.forename: J
authors.initials: J
authors.lastname: Hemet
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
authors.forename: P
authors.initials: P
authors.lastname: Leroux
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002531:Cerebellum / Q000166:cytology*; D004247:DNA / Q000096:biosynthesis; D007700:Kinetics; D008940:Mitotic Index / Q000187:drug effects; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D015282:Octreotide / Q000494:pharmacology*; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
chemical_list: D013004:Somatostatin; D004247:DNA; D015282:Octreotide; D013936:Thymidine