PCR-amplified cDNA probes for verification of differentially expressed genes.
Differential display has proven to be a powerful technique for the detection and isolation of differentially expressed genes. By generating reproducible cDNA expression patterns, it is possible to compare gene expression by two or more cell types, developmental stages or tissues and to isolate as yet unknown differentially expressed genes. A sensitive method is necessary to verify the differential expression of the isolated cDNAs. Here we describe the use of adaptor-ligated. PCR-amplified total cDNA of the two cell types compared as a probe for Southern hybridizations with the isolated cDNAs.
Ross
R
R
Department of Dermatology, Johannes Gutenberg-University, Mainz, Germany.
Kumpf
K
K
Reske-Kunz
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AB
D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes / Q000032:analysis*; D004817:Epidermis / Q000737:chemistry; D015870:Gene Expression; D005802:Genes, MHC Class II; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D051379:Mice; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism
D015342:DNA Probes; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1