[Bacterial synthesis of immunogenic epitopes of foot-and-mouth disease virus fused either to human necrosis factor or to hepatitis B core antigen].
Using recombinant DNA technology, construction and bacterial expression of genes was carried out which code for hybrid proteins, human tumor necrosis factor and hepatitis B core protein fused to immunogenic epitopes of foot-and-mouth disease virus, strains A22 and O1-194. Hybrids of tumor necrosis factor with foot-and-mouth disease antigenic determinants protected laboratory animals against the experimental challenge with a homologous strain of foot-and-mouth disease virus. Hybrid protein that contained immunogenic regions of two strains, A22 and O1-194, protected animals against infection with both A and O serotypes. Hybrid proteins based on hepatitis B virus core antigen retained the ability to assemble into core-like particles.
Nekrasova
O V
OV
Boĭchenko
V E
VE
Boldyreva
E F
EF
Borisova
G P
GP
Pumpen
P
P
Perevozchikova
N A
NA
Korobko
V G
VG
D000818:Animals; D005537:Aphthovirus / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D000939:Epitopes / Q000096:biosynthesis* / Q000235:genetics; D006168:Guinea Pigs; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D018076:DNA, Complementary; D000939:Epitopes; D006512:Hepatitis B Core Antigens; D015345:Oligonucleotide Probes; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha
Bakterial'nyĭ sintez immunogennykh épitopov virusa iashchura v sostave gibridov s faktorom nekroza opukholeĭ i kor-antigenom virusa gepatita B.