title: Effect of phosphate concentration in buffered irrigating solutions on arterial thrombosis.
abstract: The concentration of phosphate in buffered saline irrigation solutions was evaluated for its effect upon the incidence of arterial thrombosis. A rabbit thrombosis model of combined intimal abrasion and twisted pedicle in the rabbit central ear artery was used. Higher concentrations of phosphate (20 mM) in normal saline yielded a lower thrombosis rate at 7 days postoperatively (50%) in comparison to 5 mM phosphate (89% thrombosis rate; P < 0.05). A standard phosphate-buffered salt solution (Dulbecco's; 9.5 mM phosphate with potassium, calcium, and magnesium salts) also had a high thrombosis rate (91%). This study demonstrates that subtle variations in the irrigation solution can have profound effects upon arterial thrombosis.
authors.affiliation: Department of Surgery, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Brown
authors.forename: N
authors.initials: N
authors.lastname: Kania
authors.forename: L A
authors.initials: LA
authors.lastname: Lantieri
authors.forename: E N
authors.initials: EN
authors.lastname: Pasia
authors.forename: R K
authors.initials: RK
authors.lastname: Khouri
mesh_terms: D000818:Animals; D002021:Buffers; D004195:Disease Models, Animal; D004423:Ear / Q000098:blood supply; D008866:Microsurgery; D010710:Phosphates / Q000494:pharmacology*; D011817:Rabbits; D007507:Therapeutic Irrigation / Q000379:methods*; D013927:Thrombosis / Q000517:prevention & control*; D014654:Vascular Patency
chemical_list: D002021:Buffers; D010710:Phosphates