title: Lactate-bicarbonate interrelationship during exercise and recovery in lean and obese Zucker rats.
abstract: OBJECTIVE
  To determine the relationship between muscle-derived lactate at fatigue and earlier onset of fatigue in the obese rat subjected to intense exercise.
  
  
  METHODS
  Rats were subjected to a short, intense exercise protocol on a treadmill. Blood was drawn from hind leg vein and artery during exercise and up to 1 h afterwards. Assuming an exercise respiratory quotient of 1.0, the extra carbon dioxide released was computed and assumed to be displaced by equimolar amounts of lactic acid produced by the rat during exercise.
  
  
  METHODS
  Conscious female adult Zucker lean and Zucker obese rats.
  
  
  METHODS
  Oxygen consumption and carbon dioxide release. Lactate and bicarbonate levels in hind leg venous and arterial blood; balances were estimated by measuring blood flow with fluorescent microspheres. Lactate levels in periovaric white adipose tissue were also measured.
  
  
  RESULTS
  Muscle released, during exercise and post exercise roughly 2.3 mmol lactate in lean rats and 2.6 mmol in obese ones. Of these amounts, hind leg lactate release accounted for 0.40 mmol in lean rats and only 0.11 in the obese ones, which showed a release of acid (mainly lactate) elsewhere in the rats totalling about 19.9 mmol CO2 in lean rats and 4.4% in the obese ones; that is both hind quarters accounted for only 17.2% of all lactate produced in the lean rats and 4.4% in the obese ones. The amount of lactate produced by the rats was roughly similar. White adipose tissue lactate levels (in the basal state and after exercise) were much higher than could be expected from blood sources alone, indicating an active production of lactate.
  
  
  CONCLUSIONS
  Fatigue appears earlier in the obese rats than in lean ones because of loss of buffering ability caused by massive extra-muscular glycolysis (probably in adipose tissue) and lactate production triggered by exercise-induced adrenergic stimulation.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ardévol
authors.forename: C A
authors.initials: CA
authors.lastname: Remesar
authors.forename: J A
authors.initials: JA
authors.lastname: Fernández-López
authors.forename: M
authors.initials: M
authors.lastname: Alemany
mesh_terms: D000818:Animals; D001158:Arteries / Q000737:chemistry; D001639:Bicarbonates / Q000097:blood*; D002245:Carbon Dioxide / Q000378:metabolism; D005260:Female; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000097:blood*; D009765:Obesity / Q000097:blood / Q000503:physiopathology*; D010101:Oxygen Consumption / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D011924:Rats, Zucker
chemical_list: D001639:Bicarbonates; D002245:Carbon Dioxide; D019344:Lactic Acid