Reconstitution of two isoforms of the human interleukin-11 receptor and comparison of their functional properties.
Long-term stable Ba/F3 transfectants (B13R alpha1 and B13R alpha2) expressing two isoforms of the human IL-IIR alpha receptor (alpha1 full length or alpha2 lacking the cytoplasmic domain) in combination with human gp130 were established. IL-11R alpha1 and IL-11R alpha2 were each expressed and detected as three bands upon Western blot analysis, with apparent molecular masses in agreement with those of the polypeptide backbone (47 and 44 kDa, respectively) with no, one or two N-linked sugars. B13R alpha1 and B13R alpha2 bound IL-11-thioredoxin with similar efficiencies and proliferated with superimposable dose-response curves to IL-11, demonstrating that the intracellular domain of IL-11R alpha has no significant contribution on ligand binding and signaling. Analysis of a set of anti-human gp130 mAbs confirmed the similar responsiveness of B13R alpha1 and B13R alpha2 transfectants.
Lebeau
B
B
Groupe de Recherche Cytokines et Récepteurs, Unité INSERM 463, Nantes, France.
Montero Julian
F A
FA
Wijdenes
J
J
Müller-Newen
G
G
Dahmen
H
H
Chérel
M
M
Heinrich
P C
PC
Brailly
H
H
Hallet
M M
MM
Godard
A
A
Minvielle
S
S
Jacques
Y
Y
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D017370:Interleukin-11 / Q000494:pharmacology*; D053703:Interleukin-11 Receptor alpha Subunit; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D018123:Receptors, Interleukin / Q000235:genetics / Q000378:metabolism*; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D052118:Lysosomal-Associated Membrane Protein 1
D017370:Interleukin-11; D053703:Interleukin-11 Receptor alpha Subunit; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins; C508428:IL11RA protein, human; C508429:Il11ra1 protein, mouse; C508431:Il11ra2 protein, mouse; C489116:LAMP1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1; C052255:Lamp1 protein, mouse