title: Detection of human Betaherpesvirinae in saliva and urine from immunocompromised and immunocompetent subjects.
abstract: Human cytomegalovirus (HCMV) is a well-known opportunistic agent that reactivates in human immunodeficiency virus (HIV)-seropositive subjects. Human herpesvirus 6 (HHV-6) and HHV-7 were discovered recently and, like HCMV, belong to the Betaherpesvirinae subfamily. We looked for the presence of HCMV, HHV-6, and HHV-7 by PCR with saliva and urine samples from 125 HIV-seropositive patients at different stages of HIV infection and with saliva and urine samples from 29 HIV-seronegative subjects. All three viruses were frequently detected in the saliva (overall rates of detection, 61, 43, and 63% for HCMV, HHV-6, and HHV-7, respectively) with no correlation with the stage of immune deficiency. In contrast, HCMV was detected in urine much more frequently than the two other herpesviruses (overall rates of detection, 37, 2, and 6.5% for HCMV, HHV-6, and HHV-7, respectively) and was associated with immune deficiency. This suggests that these three genetically related viruses differ from each other with regard to replication in the urinary tract.
authors.affiliation: Laboratoire de Virologie, CNRS EP 57, CERVI, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Gautheret-Dejean
authors.forename: J T
authors.initials: JT
authors.lastname: Aubin
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: J M
authors.initials: JM
authors.lastname: Huraux
authors.forename: J C
authors.initials: JC
authors.lastname: Nicolas
authors.forename: W
authors.initials: W
authors.lastname: Rozenbaum
authors.forename: H
authors.initials: H
authors.lastname: Agut
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D018141:Betaherpesvirinae / Q000302:isolation & purification*; D018791:CD4 Lymphocyte Count; D004279:DNA, Viral / Q000652:urine; D006679:HIV Seropositivity / Q000276:immunology / Q000821:virology; D006566:Herpesviridae Infections / Q000821:virology*; D016199:Herpesvirus 7, Human / Q000302:isolation & purification*; D006801:Humans; D016867:Immunocompromised Host; D012463:Saliva / Q000821:virology
chemical_list: D004279:DNA, Viral