Exogenous heparin induces fibronectin overexpression parallel to angiogenesis in the extracellular matrix of the chick embryo chorioallantoic membrane.
Heparin (HE) was injected into the allantoic sac of chick embryo eggs on the 5th day of incubation. After 48 h, a morphometric analysis of angiogenic response and an immunohistochemical investigation of fibronectin (FN) and type IV collagen immunoreactivity in developing vasculature were performed in order to verify whether HE-related choriollantoic membrane (CAM) angiogenic activity was associated with overexpression of FN and/or type IV collagen changes in CAM extracellular matrix. Data to be presented show a close relationship between HE treatment, angiogenic processes, and overexpression of FN, but not of type IV collagen in CAM extracellular matrix. They agree with other studies proving a facilitating role of FN in angiogenic processes.
Ribatti
D
D
Institute of Human Anatomy, Histology and Embryology, University of Bari, Italy.
Vacca
A
A
Costantino
F
F
Minischetti
M
M
Locci
P
P
Becchetti
E
E
Roncali
L
L
Dammacco
F
F
D000482:Allantois / Q000098:blood supply / Q000737:chemistry; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply* / Q000737:chemistry; D004625:Embryo, Nonmammalian / Q000098:blood supply; D005109:Extracellular Matrix / Q000737:chemistry / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D005353:Fibronectins / Q000032:analysis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D007150:Immunohistochemistry; D008833:Microcirculation / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000187:drug effects*
D005343:Fibrinolytic Agents; D005353:Fibronectins; D006493:Heparin