title: Isolation and mapping of karyopherin alpha 3 (KPNA3), a human gene that is highly homologous to genes encoding Xenopus importin, yeast SRP1 and human RCH1.
abstract: From a human fetal-brain cDNA library, we isolated and characterized a novel gene (KPNA3) encoding a protein highly homologous to certain nuclear transport proteins of Xenopus and human. The complete cDNA clone, designated karyopherin alpha 3, contained an open reading frame of 1,563 nucleotides encoding 521 amino acids. The predicted amino acid sequence showed 48%, 45% and 48% identity with Xenopus importin, yeast SRP1 and human RCH1, respectively. The similarities among these proteins suggest that karyopherin alpha 3 may be involved in the nuclear transport system. Eight repeats of the arm motif were well conserved among these proteins. The N-terminal region of the predicted karyopherin alpha 3 product was highly basic and the C-terminal region was strongly acidic. A 4.3-kb transcript was expressed in all adult human tissues examined by Northern blotting. The cDNA clone was assigned to chromosome band 13q14.3 by fluorescence in situ hybridization.
authors.affiliation: Otsuka GEN Research Institute, Tokushima 771-01, Japan. takeda@otsuka.genome.ad.jp
authors.forename: S
authors.initials: S
authors.lastname: Takeda
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
authors.forename: F
authors.initials: F
authors.lastname: Shimizu
authors.forename: A
authors.initials: A
authors.lastname: Kawai
authors.forename: K
authors.initials: K
authors.lastname: Shinomiya
authors.forename: S
authors.initials: S
authors.lastname: Okuno
authors.forename: K
authors.initials: K
authors.lastname: Ozaki
authors.forename: T
authors.initials: T
authors.lastname: Katagiri
authors.forename: Y
authors.initials: Y
authors.lastname: Shimada
authors.forename: M
authors.initials: M
authors.lastname: Nagata
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: A
authors.initials: A
authors.lastname: Takaichi
authors.forename: Y
authors.initials: Y
authors.lastname: Kuga
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Hishigaki
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Shin
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Hirai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D028884:Karyopherins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014981:Xenopus; D028901:alpha Karyopherins
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D006360:Heat-Shock Proteins; C106306:KPNA3 protein, human; D028884:Karyopherins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; C491115:TIR1 protein, S cerevisiae; D028901:alpha Karyopherins; C087978:karyopherin alpha 2