Application of the multi-colour FISH to interphase nuclei and metaphase spreads for simultaneous examination of monosomy 7 and trisomies 8 and 11 in acute myelocytic leukaemia (AML).
We have used the multi-colour (three) fluorescence in situ hybridization (FISH) technique based on the ratio labelling for the detection of monosomy 7 and trisomies 8 and 11 in 13 cases of acute myeloid leukaemia (AML). Two out of the 13 AML cases showed monosomy 7 and two out of the remaining cases exhibited trisomy 8 in interphase nuclei. Three of these results were confirmed by metaphase-FISH study. Trisomy 11 was not found either by the interphase FISH study or in the metaphase FISH study. These results demonstrate the potential power of multi-colour FISH using ratio labelling to produce more fluorescein colour in interphase nuclei for the detection of aneuploidies in leukaemia.
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Department of Medical Genetics, University of Selçuk, Konya, Turkey.
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D000293:Adolescent; D000328:Adult; D000782:Aneuploidy; D002675:Child, Preschool; D002880:Chromosomes, Human, Pair 11; D002897:Chromosomes, Human, Pair 7; D002898:Chromosomes, Human, Pair 8; D003582:Cytogenetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000235:genetics*; D008297:Male; D008677:Metaphase / Q000235:genetics*; D008875:Middle Aged; D009006:Monosomy / Q000175:diagnosis* / Q000235:genetics; D014314:Trisomy / Q000175:diagnosis* / Q000235:genetics