Measurement of inherent particle properties by dynamic light scattering: introducing electrorotational light scattering.
Common dynamic light scattering (DLS) methods determine the size and zeta-potential of particles by analyzing the motion resulting from thermal noise or electrophoretic force. Dielectric particle spectroscopy by common microscopic electrorotation (ER) measures the frequency dependence of field-induced rotation of single particles to analyze their inherent dielectric structure. We propose a new technique, electrorotational light scattering (ERLS). It measures ER in a particle ensemble by a homodyne DLS setup. ER-induced particle rotation is extracted from the initial decorrelation of the intensity autocorrelation function (ACF) by a simple optical particle model. Human red blood cells were used as test particles, and changes of the characteristic frequency of membrane dispersion induced by the ionophore nystatin were monitored by ERLS. For untreated control cells, a rotation frequency of 2 s-1 was induced at the membrane peak frequency of 150 kHz and a field strength of 12 kV/m. This rotation led to a decorrelation of the ACF about 10 times steeper than that of the field free control. For deduction of ERLS frequency spectra, different criteria are discussed. Particle shape and additional field-induced motions like dielectrophoresis and particle-particle attraction do not significantly influence the criteria. For nystatin-treated cells, recalculation of dielectric cell properties revealed an ionophore-induced decrease in the internal conductivity. Although the absolute rotation speed and the rotation sense are not yet directly accessible, ERLS eliminates the tedious microscopic measurements. It offers computerized, statistically significant measurements of dielectric particle properties that are especially suitable for nonbiological applications, e.g., the study of colloidal particles.
Prüger
B
B
Institute of Biology, Humboldt University, Berlin, Germany.
Eppmann
P
P
Donath
E
E
Gimsa
J
J
D004867:Equipment Design; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007834:Lasers; D008027:Light; D008838:Microcomputers; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D009761:Nystatin / Q000494:pharmacology; D012399:Rotation; D012542:Scattering, Radiation; D013696:Temperature; D013816:Thermodynamics
D009761:Nystatin