title: Down-regulation of beta3-adrenergic receptor expression in rat adipose tissue during the fasted/fed transition: evidence for a role of insulin.
abstract: The beta3-adrenergic receptor (beta3-AR) exerts a central role in the transduction of catecholamine effects in white and brown adipose tissue (WAT and BAT). A recent report has documented that insulin strongly down-regulates beta3-AR expression and catecholamine responsiveness in 3T3-F442A adipocytes [Fève, El Hadri, Quignard-Boulangé and Pairault (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 5677-5681]. In the present report we show that the rise in plasma insulin levels elicited by the fasted/fed transition is associated with a reduction in beta3-AR mRNA levels and beta-adrenergic responsiveness in WAT and BAT. beta3-AR transcripts are also decreased in adipose tissue from animals subjected for 6 h to euglycaemic hyperinsulinaemic glucose clamps. Moreover, insulin acts directly on cultured rat white and brown adipocytes to decrease beta3-AR gene expression and adenylate cyclase activity in response to beta3-AR-selective agonists. These results suggest that there is a close relationship between food intake, plasma insulin levels and beta3-AR expression.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale Unité 282, Hôpital Henri Mondor, 94010-Créteil, France.
authors.forename: K E
authors.initials: KE
authors.lastname: Hadri
authors.forename: C
authors.initials: C
authors.lastname: Charon
authors.forename: J
authors.initials: J
authors.lastname: Pairault
authors.forename: S
authors.initials: S
authors.lastname: Hauguel-De Mouzon
authors.forename: A
authors.initials: A
authors.lastname: Quignard-Boulangé
authors.forename: B
authors.initials: B
authors.lastname: Fève
mesh_terms: D016475:3T3 Cells; D000067956:Adenylyl Cyclase Inhibitors; D017667:Adipocytes / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D004435:Eating; D004983:Ethanolamines / Q000494:pharmacology; D005215:Fasting; D007328:Insulin / Q000097:blood*; D051379:Mice; D011412:Propanolamines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D022702:Receptors, Adrenergic, beta-3
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D007328:Insulin; D011412:Propanolamines; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; C057368:BRL 37344; C033394:CGP 12177