[Chromosomal localization of M/SAR from DNA of the clone lambda20p1.4 in Drosophila melanogaster salivary glands].
M/SAR of Drosophila melanogaster DNA clone gamma 20p 1.4 was localized on chromosomes by in situ hybridization of the gamma 20p 1.4 DNA fragment and its deletion mutants with salivary gland chromosomes of third instar larvae of the lines Canton S and Oregon R. The M/SAR sequence, capable of specifically binding to lamin in vitro, was detected in the pericentric regions 20CD, 40EF, 41AB, the proximal part of region 81 of chromosome 3, and region 101 of chromosome 4, which attach to the nuclear envelope in cells of D. melanogaster salivary glands, and in region 49D, which does not come into contact with the nuclear periphery. This is the first M/SAR localized in pericentric chromatin of D. melanogaster.
Sharakhov
I V
IV
Bogachev
S S
SS
Fisher
P A
PA
D000818:Animals; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D053938:DNA Fragmentation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D017403:In Situ Hybridization; D034882:Lamins; D007814:Larva / Q000235:genetics; D009685:Nuclear Envelope / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D015183:Restriction Mapping; D012469:Salivary Glands / Q000502:physiology*
D004268:DNA-Binding Proteins; D034882:Lamins; D009687:Nuclear Proteins; D004247:DNA
Khromosomnaia lokalizatsiia M/SAR DNK klona lambda20p1.4 v sliunnykh zhelezakh Drosophila melanogaster.