Role of proliferation for intercellular induction of apoptosis.
We have recently described induction of apoptosis of transformed target cells by TGF-beta-treated nontransformed effector cells as a potential novel control step in oncogenesis. Here we study the role of proliferation of both cell types for the efficiency of induction of apoptosis. Inhibition of proliferation of transformed target cells by gamma irradiation or colchicine treatment did not affect their sensitivity to induction of apoptosis by TGF-beta-treated nontransformed effector cells. This finding indicates that sensitivity to intercellular induction of apoptosis is not related to cell cycle control. In contrast, the ability of nontransformed effector cells to induce apoptosis was dependent on their proliferation. Nontransformed cells blocked by gamma irradiation, colchicine treatment, or thymidine treatment were no longer able to induce apoptosis of transformed target cells. This inability seems to be partially due to substances that are released from proliferation-inhibited nontransformed effector cells and that interfere with induction of apoptosis.
Bassler
D
D
Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Germany.
Brauns
H D
HD
Bauer
G
G
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D002455:Cell Division / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D006131:Growth Inhibitors / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D017077:Culture Media, Conditioned; D006131:Growth Inhibitors; D016212:Transforming Growth Factor beta; D003078:Colchicine