title: The integration host factor-DNA complex upstream of the early promoter of bacteriophage Mu is functionally symmetric.
abstract: Inversion of the ihf site in the promoter region of the early promoter of bacteriophage Mu did not influence the integration host factor (IHF)-mediated functions. IHF bound to this inverted site could counteract H-NS-mediated repression, directly activate transcription, and support lytic growth of bacteriophage Mu. This implies that the IHF heterodimer and its asymmetrical binding site form a functionally symmetrical complex.
authors.affiliation: Laboratory of Molecular Genetics, Leiden University, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: van Ulsen
authors.forename: M
authors.initials: M
authors.lastname: Hillebrand
authors.forename: L
authors.initials: L
authors.lastname: Zulianello
authors.forename: P
authors.initials: P
authors.lastname: van de Putte
authors.forename: N
authors.initials: N
authors.lastname: Goosen
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D016384:Consensus Sequence; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D005686:Galactokinase / Q000096:biosynthesis; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D011993:Recombinant Fusion Proteins; D005686:Galactokinase