Expression of GABA(A) receptor subunit mRNAs by layer V pyramidal cells of the rat primary visual cortex.
The expression of the GABA(A) receptor subunit mRNAs by layer V pyramidal neurons of the primary visual cortex and cerebellar Purkinje cells was analysed by single-cell reverse transcription of the mRNAs and amplification of the resulting cDNAs by the polymerase chain reaction. Neurons were identified by infrared videomicroscopy, and GABA(A)-mediated miniature inhibitory postsynaptic currents were recorded. In Purkinje cells, alpha1, beta2, beta3, gamma2S and gamma2L subunit mRNAs were detected within a single cell. In layer V pyramidal cells, a total of ten GABA(A) receptor subunit mRNAs could be detected, with a mean of seven subunit mRNAs per cell, suggesting GABA(A) receptor heterogeneity within a single pyramidal cell.
Ruano
D
D
Institut Alfred Fessard, CNRS UPR 2212, Gif sur Yvette, France.
Perrais
D
D
Rossier
J
J
Ropert
N
N
D000818:Animals; D002531:Cerebellum / Q000502:physiology; D017931:DNA Primers; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008297:Male; D008564:Membrane Potentials; D016133:Polymerase Chain Reaction; D011689:Purkinje Cells / Q000502:physiology; D017966:Pyramidal Cells / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000737:chemistry; D014158:Transcription, Genetic; D014793:Visual Cortex / Q000502:physiology*
D017931:DNA Primers; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011963:Receptors, GABA-A