Reversed-phase high-performance liquid chromatographic separation of LY309887 (thienyl-5,10-dideazatetrahydrofolate) stereoisomers using beta-cyclodextrin as a mobile phase additive.
The separation of the four stereoisomers of LY309887 (thienyl-5,10-dideazatetrahydrofolate) is studied here using commercially available high-performance liquid chromatography column technology. The selectivity needed to resolve the four stereoisomers is provided by an achiral reversed-phase column used with beta-cyclodextrin as a mobile phase additive. The separation is dependent on triethylamine and beta-cyclodextrin concentration in the buffer portion of the eluent, eluent strength, and buffer pH.
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Chemical Process Research and Development, Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013763:Tetrahydrofolates / Q000737:chemistry / Q000302:isolation & purification*
C105065:6R-2',5'-thienyl-5,10-dideazatetrahydrofolic acid; D004791:Enzyme Inhibitors; D013763:Tetrahydrofolates