title: The protein encoded by the MFT1 gene is a targeting factor for mitochondrial precursor proteins, and not a core ribosomal protein.
abstract: Yeast cells harboring mft1 mutations are compromised in mitochondrial protein targeting, and Mft1p has previously been identified as a ribosomal protein. However, two genes, PLC2 and YML062C, are present in the MFT1 locus, and we show that mft1 mutant cells are compromised in the function of the cytosolic protein encoded by YML062C. The ribosomal protein (YS3a) is actually encoded by the tightly linked PLC2 gene, and does not play a role in targeting proteins to the mitochondria.
authors.affiliation: School of Biochemistry, La Trobe University, Bundoora, Australia.
authors.forename: T
authors.initials: T
authors.lastname: Beilharz
authors.forename: T
authors.initials: T
authors.lastname: Beddoe
authors.forename: K
authors.initials: K
authors.lastname: Landl
authors.forename: P
authors.initials: P
authors.lastname: Cartwright
authors.forename: T
authors.initials: T
authors.lastname: Lithgow
mesh_terms: D000595:Amino Acid Sequence; D002451:Cell Compartmentation; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008040:Genetic Linkage; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011485:Protein Binding; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D015003:Yeasts / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D011498:Protein Precursors; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C068359:MFT1 protein, S cerevisiae