Concomitant presence of p16/cyclin-dependent kinase 4 and cyclin D/cyclin-dependent kinase 4 complexes in LNCaP prostatic cancer cell line.
The cyclin D/cyclin-dependent kinase (CDK)/CDK-inhibitory proteins/retinoblastoma protein (pRb) pathway is hypothesized to control the G1-S check point. The role of this pathway is reported to be different depending on the status of pRb. In the present study, we examined nine human urological tumor cell lines. Cells lacking functional pRb expressed p16, instead of forming cyclin D/ CDK4 complex. In the LNCaP prostatic cancer cell line, however, both p16/CDK4 and cyclin D/ CDK4 complexes were present independently, probably because of partial loss of pRb. In view of the concomitant presence of the incompatible complexes, LNCaP should provide us with a valuable model for the study of this pathway in cancer cells.
Itoh
N
N
Department of Urology, Faculty of Medicine, Kyoto University, Kyoto.
Kakehi
Y
Y
Akao
T
T
Kinoshita
H
H
Okada
Y
Y
Yoshida
O
O
D002292:Carcinoma, Renal Cell; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002453:Cell Cycle; D056741:Cyclin D; D051358:Cyclin-Dependent Kinase 4; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis* / Q000302:isolation & purification; D016213:Cyclins / Q000096:biosynthesis* / Q000302:isolation & purification; D016193:G1 Phase; D006801:Humans; D007680:Kidney Neoplasms; D008297:Male; D011471:Prostatic Neoplasms; D011485:Protein Binding; D011518:Proto-Oncogene Proteins; D016160:Retinoblastoma Protein / Q000096:biosynthesis* / Q000172:deficiency / Q000302:isolation & purification; D016196:S Phase; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms
D002352:Carrier Proteins; D056741:Cyclin D; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins; D011518:Proto-Oncogene Proteins; D016160:Retinoblastoma Protein; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases