Cell mediated immunity induced in mice by HPV 16 L1 virus-like particles.
Recombinant human papillomavirus (HPV) type 16 L1 virus-like particles (VLPs) expressed in the baculovirus system were used to investigate the cellular immune response to human papillomavirus type 16. The cell-mediated immune response was evaluated through immunization of mice with HPV 16 L1 virus-like particles using a lymphoproliferation assay and cytokine production and cytometric analysis of lymphocyte subsets. A significant proliferative response was observed which was associated with secretion of both interferon-gamma and interleukin-2. FACS analysis of splenic lymphocytes revealed that CD8+ T-cells were increased in the immunized mice. These results demonstrate that HPV 16 L1 VLPs induce a T-cell response characterized by a Th1 profile and confirm that the HPV 16 VLP is a reasonable candidate for vaccine development.
Dupuy
C
C
Laboratoire d'Immunologie des Maladies Infectieuses, Faculté de Pharmacie, Tours, France.
Buzoni-Gatel
D
D
Touze
A
A
Le Cann
P
P
Bout
D
D
Coursaget
P
P
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D036022:Capsid Proteins; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D015848:Interleukin-5 / Q000032:analysis; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*; D010641:Phenotype; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D036022:Capsid Proteins; D007376:Interleukin-2; D015848:Interleukin-5; D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins; D015847:Interleukin-4; C063317:L1 protein, Human papillomavirus type 16; D007371:Interferon-gamma