title: Calcium-dependent and -independent binding of the pentraxin serum amyloid P component to glycosaminoglycans and amyloid proteins: enhanced binding at slightly acid pH.
abstract: Serum amyloid P component (SAP), a member of the pentraxin family of proteins, binds calcium-dependently to several ligands including glycosaminoglycans (GAG's). We have investigated the influence of pH on the Ca2(+)-dependent binding of SAP to solid phase GAG's and amyloid fibril proteins (AA and beta2M) by ELISA. An increase in the dose-dependent binding of SAP to heparan sulfate, AA-protein and beta2M was observed as the pH decreased from 8.0 to 5.0. Furthermore, a lower, but significant Ca2(+)-independent binding of SAP to heparan sulfate, dermatan sulfate, AA protein and the amyloid precursor protein beta2M was observed. This binding was also enhanced at slightly acid pH, most pronounced at pH 5.0. The results of this study indicate that SAP can exhibit both Ca2(+)-dependent and -independent binding to ligands involved in amyloid fibril formation and that the binding is enhanced under conditions of slightly lowered pH.
authors.affiliation: Department of Medical Microbiology, Odense University, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Danielsen
authors.forename: I J
authors.initials: IJ
authors.lastname: Sørensen
authors.forename: M
authors.initials: M
authors.lastname: Nybo
authors.forename: E H
authors.initials: EH
authors.lastname: Nielsen
authors.forename: B
authors.initials: B
authors.lastname: Kaplan
authors.forename: S E
authors.initials: SE
authors.lastname: Svehag
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011498:Protein Precursors / Q000378:metabolism*; D000683:Serum Amyloid P-Component / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000682:Amyloid; D006025:Glycosaminoglycans; D011498:Protein Precursors; D000683:Serum Amyloid P-Component; D002118:Calcium