Functional roles of protein domains on rice alpha-amylase activity.
Characteristics of two rice alpha-amylases Amy1A and Amy3D, and those of two chimeric enzymes Amy1A/3D and Amy3D/1A, engineered from the two isozymes, were compared in the light of the functional roles of protein domains in alpha-amylase. The enzymes that have an Amy1A-type N-terminal domain, Amy1A and Amy1A/3D, showed high activity against soluble starch, while the enzymes that have an Amy3D-type barrel structure, Amy3D and Amy1A/3D, showed high activity in oligosaccharide hydrolysis. Rigidity of protein folding also significantly affected the enzyme activity in both soluble starch and oligosaccharide hydrolysis. Thus, the present work suggests that the structure of the N-terminal domain is important for stability and soluble starch hydrolysis, while the barrel structure that forms the active site significantly affects enzyme activities in oligosaccharide degradation. We have already characterized two rice alpha-amylase isozymes, Amy1A and Amy3D, and a chimeric enzyme engineered from these two isozymes, Amy1A/3D (Terashima et al. 1995, 1996a,b). In spite of the high homology (70%) of their amino acid sequences, Amy1A and Amy3D showed distinct differences in their enzymatic characteristics. The chimeric enzyme Amy1A/3D, which consists of an Amy1A-type N-terminal domain and an Amy3D-type barrel structure, inherited enzymatic characteristics from the both isozymes. In this work, one other chimeric enzyme, Amy3D/1A, which is the counterpart of Amy1A/3D, has been characterized. The characteristics of these four enzymes are discussed in the light of the functional roles of protein domains.
Terashima
M
M
Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Japan.
Hosono
M
M
Katoh
S
S
D000681:Amylases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression; D006868:Hydrolysis; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013213:Starch / Q000378:metabolism; D013379:Substrate Specificity
D007527:Isoenzymes; D009844:Oligosaccharides; D013213:Starch; D000681:Amylases