An improved, ultrasensitive method for the detection of IgM oligoclonal bands in cerebrospinal fluid.
A new, 10-fold more sensitive method, based on an improved immunofixation technique, has been devised to detect oligoclonal IgM bands in unconcentrated cerebrospinal fluid (CSF). Using agarose gel electrophoresis, 5 microliters of an unconcentrated sample containing oligoclonal bands was separated and blotted on to a polyvinyldifluoride membrane. To visualise the pattern, a peroxidase-labelled double-antibody technique was used. No prior concentration of CSF was needed and the process required only 5 h. The technique may prove very useful in diagnosing an early intrathecal immune response.
Rijcken
C A
CA
Department of Neurobiochemistry, University Hospital Groningen, Netherlands. C.A.W.Rijcken@farmedu.rug.nl
Thompson
E J
EJ
Teelken
A W
AW
D003429:Cross Reactions; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005404:Fixatives; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood / Q000134:cerebrospinal fluid*; D007136:Immunoglobulins / Q000097:blood / Q000134:cerebrospinal fluid*; D008567:Membranes, Artificial; D043025:Oligoclonal Bands; D011145:Polyvinyls; D012680:Sensitivity and Specificity; D005465:Fluorocarbon Polymers
D005404:Fixatives; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D008567:Membranes, Artificial; D043025:Oligoclonal Bands; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers