Identification of Listeria species by a semi-nested polymerase chain reaction.
Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welshimeri.
Manzano
M
M
Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
Cocolin
L
L
Ferroni
P
P
Gasparini
V
V
Narduzzi
D
D
Cantoni
C
C
Comi
G
G
D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008087:Listeria / Q000235:genetics / Q000302:isolation & purification*; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*