title: Analysis of tumor-infiltrating lymphocytes in cutaneous squamous cell carcinoma.
abstract: OBJECTIVE
  To characterize tumor-infiltrating lymphocytes (TILs) within lesions of cutaneous squamous cell carcinoma (SCC) and related disorders.
  
  
  METHODS
  Case series with 1- and 2-color immunohistologic, molecular biological analysis of T-cell clonality and in vitro cytotoxicity assays.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  Twenty-one patients, including 6 with actinic keratoses, 4 with SCC in situ, and 11 with invasive SCC.
  
  
  RESULTS
  CD8+ TILs were present within lesions of cutaneous SCC and AK. These cells constituted a variable minority of the total T-cell infiltrate, and many expressed a phenotype consistent with major histocompatibility complex-restricted cytotoxic T lymphocytes: CD3+, TIA1+, CD16-, CD56-, CD57-. They also expressed HLA-DR, suggesting their activation in vivo. Virtually all T cells were T-cell receptor (TCR)-beta + delta, indicating that they expressed the TCR-alpha beta protein heterodimer. Molecular biological analysis of TCR-gamma gene rearrangements by the polymerase chain reaction and denaturing gradient gel electrophoresis technique indicated that the TILs were polyclonal. Functional studies suggested that TILs derived from SCC lesions were cytotoxic for autologous tumor cell targets.
  
  
  CONCLUSIONS
  Tumor-infiltrating lymphocytes within cutaneous SCC lesions contain a subpopulation of polyclonal, major histocompatibility complex-restricted cytotoxic T lymphocytes expressing the TCR-alpha beta heterodimer.
authors.affiliation: Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: A C
authors.initials: AC
authors.lastname: Haeffner
authors.forename: K
authors.initials: K
authors.lastname: Zepter
authors.forename: C A
authors.initials: CA
authors.lastname: Elmets
authors.forename: G S
authors.initials: GS
authors.lastname: Wood
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002860:Chromium Radioisotopes / Q000493:pharmacokinetics; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D002860:Chromium Radioisotopes