Expression and identification of aberrant c-kit transcripts in human cancer cells.
We have previously cloned and sequenced a novel 3.5 kb c-kit mRNA expressed in a colon carcinoma cell line Colo201. Here we examined the expression of this truncated form of c-kit in 14 gastrointestinal cancer cells and 16 hematopoieic cancer cells by RT-PCR. Expression of the aberrant c-kit transcript was observed in various cancer cell lines. Furthermore, a new transcript which is 78 bp shorter than the transcript previously described was identified and characterized. These results indicate that two kinds of aberrant c-kit transcript produced by alternative promoter in intron 15 are expressed in human cancer cells.
Takaoka
A
A
First Department of Internal Medicine, Sapporo Medical University, Chuo-ku, Japan.
Toyota
M
M
Hinoda
Y
Y
Itoh
F
F
Mita
H
H
Kakiuchi
H
H
Adachi
M
M
Imai
K
K
D000230:Adenocarcinoma / Q000378:metabolism*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D005770:Gastrointestinal Neoplasms / Q000235:genetics / Q000378:metabolism*; D019337:Hematologic Neoplasms / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D019009:Proto-Oncogene Proteins c-kit