Horseradish peroxidase permeation from the capillaries of the stria vascularis after inoculation of endotoxin into the middle ear.
Escherichia coli-derived endotoxin was inoculated in the middle ear of guinea pigs 24 hours after being injected intraperitoneally. Twenty-four hours after the middle ear inoculation, horseradish peroxidase (HRP) was injected via the femoral vein and the permeability of HRP through the capillaries of the stria vascularis and the destination of the leaked HRP were examined. A large amount of HRP leaked out of the capillary through he opened endothelial cell junctions and penetrated the enlarged intercellular spaces. Leaked HRP entered the pinocytotic vesicles of the intermediate cells. Even slightly degenerated intermediate cells retained this function. The HRP penetrated the spongelike structure of the marginal cells leading to the intercellular space. This structure was not observed without endotoxin. The HRP could not pass the cochlear duct through the tight junctions between marginal cells. Blood sludging was observed in the strial capillaries. It appeared more frequently in the upper three turns than in the basal turn. The HRP leakage out of the capillaries was observed not only in the upper three turns but also in the basal turn.
Watanabe
K
K
Department of Otorhinolaryngology, Koshigaya Hospital, Dokkyo University School of Medicine, Japan.
Tanaka
Y
Y
D000818:Animals; D002199:Capillary Permeability; D004432:Ear, Middle / Q000187:drug effects*; D004731:Endotoxins / Q000494:pharmacology*; D006168:Guinea Pigs; D006735:Horseradish Peroxidase / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008854:Microscopy, Electron; D013316:Stria Vascularis / Q000378:metabolism / Q000648:ultrastructure
D004731:Endotoxins; D006735:Horseradish Peroxidase