Regulation of the metabolism of plasma lipoproteins by apolipoprotein A-II.
Mouse apolipoprotein (apo) A-II has three variants (type A, B, and C) among inbred strains. To clarify the role of ApoA-II in the metabolism of high density lipoproteins (HDL), we constructed a new congenic mouse strain (P1.R1-Apoa2b) with type B ApoA-II of the SAMR1 strain on the genetic background of the SAMP1 strain, and examined it together with another ApoA-II congenic strain (R1.P1-Apoa2c) containing type C ApoA-II of the SAMPI strain on the SAMR1 strain and the parental SAMP1 and SAMR1 strains. Genetic characterization of the congenic strains indicated that only small regions surrounding the ApoA-II gene of the parental strains had been transferred. The strains with Apoa2c had lower plasma concentrations of HDL and ApoA-II, and a smaller HDL particle size than strains with Apoa2b. We detected no significant differences in the mRNA levels of ApoA-II or in the in vitro translational efficiency of the ApoA-II mRNA among the four strains. These findings suggested that the differences in the post-translational modification or efficiency of secretion between the Apoa2b and Apoa2c protein regulates the ApoA-II concentration which in turn determines the concentration and size of HDL in mice.
Wang
J
J
Department of Senescence Biology, Chest Disease Research Institute, Kyoto University, Sakyo-ku, Japan.
Kitagawa
K
K
Kitado
H
H
Kogishi
K
K
Matsushita
T
T
Hosokawa
M
M
Higuchi
K
K
D000821:Animal Feed; D000818:Animals; D016633:Apolipoprotein A-II / Q000097:blood / Q000235:genetics*; D004041:Dietary Fats / Q000008:administration & dosage*; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry; D051379:Mice; D008817:Mice, Mutant Strains; D011110:Polymorphism, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis
D016633:Apolipoprotein A-II; D004041:Dietary Fats; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D012333:RNA, Messenger