Mg2+- or Mn2+-dependent endonuclease activities of human myeloid leukemia cells capable of producing nucleosomal-size DNA fragmentation.
The presence of at least two distinct Mg2+- or Mn2+-dependent, Ca2+-independent endonuclease activities was shown in the myeloid leukemia cell line P39. One of them was recovered from nuclear extracts and the other from a cytoplasmic fraction. The molecular size of the former was 30 kDa in both gel filtration and activity gel and that of the latter approximately 130-140 kDa in gel filtration and 65-70 kDa in activity gel. These two activities were almost completely inhibited by 0.1 mM ZnCl2 or 0.1 mM aurintricarboxylic acid, common inhibitors of apoptosis. Both could produce nucleosomal-size DNA fragmentation when incubated with diethyl-pyrocarbonate-treated nuclei as substrates, and the pattern of cleavage was 3'-OH and 5'-P. Taken together, either or both of these activities may be associated with apoptosis of myeloid leukemia cells.
Kawabata
H
H
Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
Anzai
N
N
Masutani
H
H
Hirama
T
T
Hishita
T
T
Dodo
M
M
Masuda
T
T
Yoshida
Y
Y
Okuma
M
M
D002850:Chromatography, Gel; D053938:DNA Fragmentation; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D007951:Leukemia, Myeloid / Q000201:enzymology* / Q000473:pathology; D008274:Magnesium / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*
D009707:Nucleosomes; D008345:Manganese; D004720:Endonucleases; D008274:Magnesium