Possible involvement of nonbilayer lipids in the stimulation of the activity of cytochrome P450SCC (CYP11A1) and its propensity to induce vesicle aggregation.
Phosphatidylcholines with saturated branched fatty acyl chains substituted in the 2-positions of the main chains have a significant stimulating effect on both the side chain cleavage activity of cytochrome P450SCC and the ability of P450SCC to induce a specific vesicle aggregation in lipid-vesicle reconstituted systems. Stimulation increases with increasing content of the branched phosphatidylcholines and is sensitive to its acyl chain structure and composition. Because branched phosphatidylcholines containing 2-alkyl substituted fatty acid chains belong to a new class of phosphatidylcholines forming inverted nonbilayer phases the results indicate that nonbilayer lipids might play an important role in the function of mitochondrial P450SCC.
Schwarz
D
D
Max Delbruck Centrum for Molecular Medidine, Berlin-Buch, Germany.
Kisselev
P
P
Wessel
R
R
Pisch
S
S
Bornscheuer
U
U
Schmid
R D
RD
D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism*; D004134:Dimyristoylphosphatidylcholine; D008081:Liposomes / Q000378:metabolism*; D009391:Nephelometry and Turbidimetry; D010713:Phosphatidylcholines / Q000737:chemistry / Q000494:pharmacology*
D008081:Liposomes; D010713:Phosphatidylcholines; D002786:Cholesterol Side-Chain Cleavage Enzyme; C017251:1,2-oleoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine