title: Description of a new HLA-E (E*01031) allele and its frequency in the Spanish population.
abstract: An HLA-E polymorphism study by oligotyping and DNA sequencing was carried out in the Spanish population. As a result, a new HLA-E allele (E*01031) initially assigned by polymerase chain reaction oligotyping as E*0104 was found. This allele presents a synonymous change at codon 77 (AAT-->AAC; Asn) when compared with the E*01032 allele. This position is located in the alpha-helix (alpha 1-domain) and is involved in the peptide binding region of the hypothetical HLA-E molecule. Among 60 Spanish individuals, HLA-E*0101 presents the highest phenotype frequency, followed in decreasing order by E*01032, E*01031 (new allele), and E*0102. Also, new partial intron 1 and complete intron 2 sequences from E*0101, E*01031, and E*01032 are described; the sequences are identical among the three forms. However, the intron 2 sequence of the E*0102 allele bears a two-base deletion not found in apes.
authors.affiliation: Department of Immunology, Hospital 12 de Octubre, Universidad Complutense, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Gomez-Casado
authors.forename: J
authors.initials: J
authors.lastname: Martinez-Laso
authors.forename: G
authors.initials: G
authors.lastname: Vargas-Alarcón
authors.forename: P
authors.initials: P
authors.lastname: Varela
authors.forename: N
authors.initials: N
authors.lastname: Diaz-Campos
authors.forename: M
authors.initials: M
authors.lastname: Alvarez
authors.forename: R
authors.initials: R
authors.lastname: Alegre
authors.forename: A
authors.initials: A
authors.lastname: Arnaiz-Villena
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D005787:Gene Frequency / Q000276:immunology*; D005805:Genes, MHC Class I / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000276:immunology; D013030:Spain; D000097625:HLA-E Antigens
chemical_list: D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens