Regional variations of muscle fibre characteristic in m. semitendinosus of growing cattle.
The objectives of the present study were to elaborate an intra-muscular profile of metabolic enzyme equipment, contractile and morphometric features along the longitudinal axis of m. semitendinosus at various ages throughout the growth phase. Thirty-seven male Montbéliard cattle, about half of them castrated, were representatively allocated to various slaughter dates, scheduled at 4, 8, 12 and 16 months of age. Samples were collected from proximal, medial and distal locations of m. semitendinosus. Isocitrate dehydrogenase (aerobic metabolism) and lactate dehydrogenase (anaerobic metabolism) were measured spectrophotometrically. Contractile muscle type was classified by quantification of myosin heavy chain I isoform proportion using the sensitive enzyme-linked immunosorbent assay. Mean muscle fibre area was obtained on histologically-stained cross-sections utilizing an image analysis system. Our results indicate the existence of a regular intra-muscular pattern of muscle fibre traits along the length of m. semitendinosus, with decreasing glycolytic activities and concomitantly an increase in oxidative capacity towards the distal extremity. The metabolic characteristics were in good agreement with decreasing cross-sectional muscle fibre areas and the slow myosin heavy chain I isoform proportion becoming gradually more abundant from proximal to distal regions of the muscle. Moreover, the observed gradient was found to be closely related to age and diminished with advanced physiological maturity. At the final slaughter age (16 months) no differences among the distinct portions were detected, m. semitendinosus was longitudinally homogeneous in all the characteristics studied.
Brandstetter
A M
AM
Laboratoire Croissance et Métabolismes des Herbivores, U.R. Croissance Musculaire, INRA, Centre de Clermont-Fd./Theix, France.
Picard
B
B
Geay
Y
Y
D000375:Aging / Q000378:metabolism; D000818:Animals; D002417:Cattle / Q000033:anatomy & histology / Q000254:growth & development*; D004797:Enzyme-Linked Immunosorbent Assay; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000201:enzymology / Q000648:ultrastructure*; D018995:Myosin Heavy Chains / Q000032:analysis
D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D018995:Myosin Heavy Chains