Interaction of dystrophin fragments with model membranes.
The interaction with membrane lipids of recombinant fragments of human dystrophin, corresponding to a single structural repeating unit of the rod domain, was examined. Surface plasmon resonance, constant-pressure isotherms in a Langmuir surface film balance, and interfacial rheology were used to observe binding of the polypeptides and its effects on the properties of the lipid film. Modification of the monolayer properties was found to depend on the presence of phosphatidylserine in the lipid mixture and on the native tertiary fold of the polypeptide; thus a fragment with the minimum chain length required for folding (117 residues) or longer caused a contraction of the surface area at constant pressure, whereas fragments of 116 residues or less had no effect. The full extent of contraction was reached at a surface concentration of lipid corresponding to an average area of about 42 A2 per lipid molecule. A dystrophin fragment with the native, folded conformation induced a large increase in surface shear viscosity of the lipid film, whereas an unfolded fragment had no effect. Within a wide range of applied shear, the shear viscosity remained Newtonian. Binding of liposomes to immobilized dystrophin fragments could be observed by surface plasmon resonance and was again related to the conformational state of the polypeptide and the presence of phosphatidylserine in the liposomes. Our results render it likely that intact dystrophin interacts directly and strongly with the sarcolemmal lipid bilayer and grossly modifies its material properties.
DeWolf
C
C
Department of Chemical Engineering, Imperial College, London, England.
McCauley
P
P
Sikorski
A F
AF
Winlove
C P
CP
Bailey
A I
AI
Kahana
E
E
Pinder
J C
JC
Gratzer
W B
WB
D001665:Binding Sites; D055592:Biophysical Phenomena; D001703:Biophysics; D016189:Dystrophin / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry; D008567:Membranes, Artificial; D008956:Models, Chemical; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013499:Surface Properties; D014783:Viscosity
D016189:Dystrophin; D008081:Liposomes; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010446:Peptide Fragments; D011994:Recombinant Proteins