title: Colocalization of parvalbumin and calbindin D-28k in neurons including chandelier cells of the human temporal neocortex.
abstract: Chandelier cells are cortical GABAergic interneurons with a unique synaptic specificity enabling them to exert a strong inhibitory influence on pyramidal cells. By using immunocytochemistry for the calcium-binding protein calbindin D-28k in the human temporal neocortex, we have found numerous immunoreactive processes that were identified as chandelier cell axon terminals. This was a striking find since in previous immunocytochemical studies of the primate neocortex, chandelier cell axon terminals had been shown to be immunoreactive for another calcium-binding protein, parvalbumin, and colocalization studies indicate that parvalbumin and calbindin are present in almost completely separate neuronal populations. Here, we present double-label immunofluorescence experiments showing that parvalbumin and calbindin immunoreactivities are colocalized in certain neurons that include a subpopulation of chandelier cells whose cell bodies are located mainly in layers V and VI of the human temporal neocortex. The results suggest a selective laminar distribution of neurochemical subtypes of chandelier cells which is a peculiar feature of the organization of the human neocortex.
authors.affiliation: Instituto Cajal (CSIC), Madrid, Spain.
authors.forename: M R
authors.initials: MR
authors.lastname: del Río
authors.forename: J
authors.initials: J
authors.lastname: DeFelipe
mesh_terms: D000293:Adolescent; D000328:Adult; D064026:Calbindins; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007150:Immunohistochemistry; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000502:physiology / Q000648:ultrastructure; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013702:Temporal Lobe / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*
chemical_list: D064026:Calbindins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G