Expression of the highly toxic centromere binding protein CENP-B in E. coli using the pET system in the absence of the inducer IPTG.
Bocanegra
J A
JA
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Cádiz, Spain.
Bejarano
L A
LA
Valdivia
M M
MM
D000818:Animals; D001324:Autoantigens; D015153:Blotting, Western; D002503:Centromere / Q000378:metabolism*; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D006224:Cricetinae; D004268:DNA-Binding Proteins; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D016133:Polymerase Chain Reaction
D001324:Autoantigens; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D007544:Isopropyl Thiogalactoside; D015245:Deoxyribonuclease BamHI; C037106:endodeoxyribonuclease NdeI; D015252:Deoxyribonucleases, Type II Site-Specific