title: In a vaccine model, selected substitution of a highly stimulatory T cell epitope of hen's egg lysozyme into a Salmonella flagellin does not result in a homologous, specific, cellular immune response and may alter the way in which the total antigen is processed.
abstract: A 13 amino acid peptide corresponding to a potent BALB/c mouse T cell epitope of hen's egg lysozyme (HEL) was substituted singly at five sites in the d flagellin of Salmonella muenchen. The resulting chimeric proteins were unable to expand T cells capable of being stimulated by the HEL epitope and induced T cell populations which either failed to respond or responded at a low level to a normally highly stimulatory flagellin T cell epitope that was present in all chimeras. The results suggested that substitution of a T cell epitope in flagellin may alter the processing of the resulting immunogen.
authors.affiliation: Department of Microbiology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Vanegas
authors.forename: N E
authors.initials: NE
authors.lastname: Street
authors.forename: T M
authors.initials: TM
authors.lastname: Joys
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000939:Epitopes / Q000276:immunology* / Q000494:pharmacology; D005408:Flagellin / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009113:Muramidase / Q000276:immunology* / Q000494:pharmacology; D012475:Salmonella / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014436:Typhoid-Paratyphoid Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology
chemical_list: D000939:Epitopes; D014436:Typhoid-Paratyphoid Vaccines; D014614:Vaccines, Synthetic; D005408:Flagellin; D009113:Muramidase