title: Interleukin (IL)-2 deficiency aggravates the defect of natural killer cell activity in AIDS patients.
abstract: A decrease in natural killer (NK) cell activity is a common feature of the immune dysfunction found in patients with human immunodeficiency virus (HIV)-induced acquired immune deficiency syndrome (AIDS). The present study was aimed at exploring the NK and the lymphokine-activated killer (LAK) cell activities of lymphocytes from HIV-seropositive subjects. The in vitro production of interleukins (IL-2 and IL-10) in response to mitogens was also studied. Two groups of HIV-seropositive subjects were studied: asymptomatic and AIDS patients. Controls were normal blood donors. The NK cell activity in peripheral blood mononuclear cells (PBMC) from AIDS patients was significantly lower than that in PBMC from both HIV-seronegative subjects and asymptomatic patients. There was no significant difference between asymptomatic patients and controls. Exposure of PBMC from all three groups of individuals to an optimal dose of IL-2 in vitro enhanced LAK cell activity. At all three effector: target cell ratios, the LAK activity in AIDS patients remained below that in normal subjects. However, the proportional increase of lytic activity with IL-2 was slightly higher in AIDS patients than in HIV-seronegative subjects. The mitogen-induced production of IL-2 was especially reduced in AIDS patients. In contrast, very high levels of mitogen-induced production of IL-10 were found in the AIDS group, as compared to asymptomatic subjects or to controls. We therefore conclude that the alteration of NK cell activity occurs at an advanced stage of HIV infection, that the reduction of cytotoxic activity is partially restored by exogenous IL-2, and that decreased production of IL-2 and increased production of IL-10 may account for part of this reduction in cytotoxicity.
authors.affiliation: Department of Transplantation and Clinical Immunology & INSERM U80, Lyon, France.
authors.forename: B
authors.initials: B
authors.lastname: Zerhouni
authors.forename: K
authors.initials: K
authors.lastname: Sanhadji
authors.forename: L
authors.initials: L
authors.lastname: Kehrli
authors.forename: J M
authors.initials: JM
authors.lastname: Livrozet
authors.forename: J L
authors.initials: JL
authors.lastname: Touraine
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000628:therapy; D000328:Adult; D016022:Case-Control Studies; D003602:Cytotoxicity, Immunologic; D018023:HIV Seronegativity / Q000276:immunology; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis / Q000172:deficiency* / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D007376:Interleukin-2; D016753:Interleukin-10