Relationship between age-dependent changes of bovine neutrophil functions and their intracellular Ca2+ concentrations.
Neutrophil functions and intracellular Ca2+ concentrations ([Ca2+]i) were evaluated in 15 Holstein cattle divided into the following 3 groups: 5 neonatal calves less than 1 week old (group 1), 5 young calves 2 to 4 weeks old (group 2) and 5 cows 2 to 3 years old (group 3). The ability of neutrophils to phagocytose Candida albicans (C. albicans) was significantly higher (p < 0.05) in neonatal and young calves than in cows, whereas the phagocytosis by neutrophils of bovine IgG-coated yeasts (IgG-yeasts) was significantly lower (p < 0.05) in neonatal and young calves than that in cows. The killing activity by neutrophils of C. albicans in neonatal and young calves was significantly lower (p < 0.05) than that in cows. Luminol dependent chemiluminescent (LDCL) responses stimulated with opsonized zymosan (OPZ), heat-aggregated IgG (H-agg.IgG) and phorbol myristate acetate (PMA) were apparently lower in neonatal and young calves than in cows. No clearly different expressions of complement receptor type 3 (CR3) on neutrophils were observed among the 3 groups of cattle, although the values due to the binding of FITC-anti-bovine IgG to neutrophils in neonatal and young calves were lower than those in group 3. The OPZ-induced [Ca2+]i of neutrophils in neonatal and young calves were significantly higher (p < 0.05) than those in cows, but they were lower in neonatal and young calves when stimulated with H-agg.IgG. These results indicate that CR3- and FcR-mediated phagocytic and killing activities of neutrophils in neonatal and young calves are different from those in cows. These phenomena may be associated with age-dependent changes in [Ca2+]i.
Higuchi
H
H
Department of Animal Health, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Nagahata
H
H
Hiroki
M
M
Noda
H
H
D000375:Aging / Q000378:metabolism* / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D002417:Cattle / Q000097:blood / Q000378:metabolism* / Q000502:physiology; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007074:Immunoglobulin G / Q000494:pharmacology; D008163:Luminescent Measurements; D016177:Macrophage-1 Antigen / Q000032:analysis; D009504:Neutrophils / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology; D011961:Receptors, Fc / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D007074:Immunoglobulin G; D016177:Macrophage-1 Antigen; D011961:Receptors, Fc; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium