title: Purification and comparison of beta-1,3-glucan binding protein from white shrimp (Penaeus vannamei).
abstract: A beta-glucan binding protein (BGBP) was identified in both white (Penaeus vannamei) and blue shrimp (P. stylirostris) plasma. White shrimp BGBP was purified by affinity chromatography using immobilized laminarin, and its molecular and biological properties were described. White shrimp BGBP is a monomeric protein with a molecular mass of 100 kDa, similar to those described for other crustacean BGBPs. White and blue shrimp BGBPs can be detected with antisera against crayfish BGBP and brown shrimp BGBP. Both amino acid composition and N-terminal sequence are markedly similar to brown shrimp (P. californiensis) and crayfish (Pacifastacus leniusculus) BGBP, indicating that this recognition protein is present in freshwater and marine crustaceans.
authors.affiliation: Center for Biological Research, La Paz, BCS, México. fvargas@cascabel.ciad.mx
authors.forename: F
authors.initials: F
authors.lastname: Vargas-Albores
authors.forename: F
authors.initials: F
authors.lastname: Jiménez-Vega
authors.forename: G M
authors.initials: GM
authors.lastname: Yepiz-Plascencia
mesh_terms: D000373:Agglutinins / Q000378:metabolism; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003400:Astacoidea; D002352:Carrier Proteins / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D003208:Concanavalin A / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005936:Glucans; D006031:Glycosylation; D037102:Lectins; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D033561:Penaeidae / Q000737:chemistry*; D011134:Polysaccharides; D017386:Sequence Homology, Amino Acid
chemical_list: D000373:Agglutinins; D000596:Amino Acids; D002352:Carrier Proteins; D005936:Glucans; D037102:Lectins; D011134:Polysaccharides; C037937:glucan-binding proteins; D003208:Concanavalin A; C008247:laminaran