Cell-type-specific modulation of Hox gene expression by members of the TGF-beta superfamily: a comparison between human osteosarcoma and neuroblastoma cell lines.
Homeobox gene expression in osteoblast-like cells was investigated using the polymerase chain reaction (PCR). A total of 13 homeobox genes was detected in U-2 OS (human osteosarcoma) and MC3T3-E1 (mouse osteoblast) cells by sequencing cloned PCR products. Using specific primers, a different pattern of Hox gene expression was shown for the neuroblastoma cell line SK-N-SH relative to U-2 OS and MC3T3-E1. Additionally, we showed that expression of HOXC6 in U-2 OS and SK-N-SH was differentially regulated by rhBMP-2, TGF-beta and activin-A. This suggests that specific Hox genes may be target genes for TGF-beta superfamily members, and allows us to further understand the complex functions of these growth factors and how they relate to growth and development.
Kloen
P
P
Department of Orthopaedic Surgery, Massachusetts General Hospital, and Harvard Medical School, Boston, USA.
Visker
M H
MH
Olijve
W
W
van Zoelen
E J
EJ
Boersma
C J
CJ
D000818:Animals; D001483:Base Sequence; D001859:Bone Neoplasms / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005801:Genes, Homeobox; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016212:Transforming Growth Factor beta