A chick bioassay approach for determining the bioavailable choline concentration in normal and overheated soybean meal, canola meal and peanut meal.
Our objectives were to use a soy protein isolate (SPI) diet containing 2-amino-2-methyl-1-propanol, an inhibitor of choline biosynthesis, to determine the bioavailable choline content of normal and overheated soybean meal (SBM), canola meal (CM) and peanut meal (PM). In the first four experiments, it was determined that weight gain of chicks fed the basal diet would respond linearly (P < 0.05) to graded levels of crystalline choline and would not respond to betaine, and that when fortified with adequate choline, no weight gain or feed intake response would occur upon addition of 100 g/kg SBM, CM or PM to the basal diet. Furthermore, addition of crystalline amino acids simulating the amino acid composition of 100 g/kg SBM did not alter the utilization of crystalline choline. In Experiment 5, feeding graded doses of choline, SBM, CM or PM resulted in linear (P < 0.05) increases in weight gain. Multiple linear regression analysis indicated bioavailable choline concentrations of 1708, 1545 and 1203 mg/kg for SBM, CM and PM, respectively. In Experiment 6, no differences (P > 0.05) in bioavailable choline concentrations occurred between normal and overheated SBM, CM or PM, and the bioavailable choline concentration of normal SBM, CM and PM was 2002, 1464 and 1320 mg/kg, respectively. Average bioavailable choline levels were 83, 24 and 76% of analytically determined choline levels in SBM, CM and PM, respectively. Canola meal, although three times as rich in total choline as SBM, has less bioavailable choline than SBM. A substantial portion of choline in SBM, CM and PM is unavailable, and overheating does not appear to decrease the bioavailability of choline in these products.
Emmert
J L
JL
Department of Animal Sciences, University of Illinois, Urbana 61801, USA.
Baker
D H
DH
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D010367:Arachis / Q000737:chemistry*; D001622:Betaine / Q000494:pharmacology; D001681:Biological Assay; D001682:Biological Availability; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D001937:Brassica / Q000737:chemistry*; D002645:Chickens / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D002794:Choline / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics*; D004032:Diet / Q000662:veterinary; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D016014:Linear Models; D008297:Male; D011897:Random Allocation; D013025:Glycine max / Q000737:chemistry*; D015430:Weight Gain / Q000187:drug effects / Q000502:physiology
D004042:Dietary Fats, Unsaturated; D001622:Betaine; D002794:Choline