title: Retention of Candida albicans on acrylic resin and silicone of different surface topography.
abstract: BACKGROUND
  The adhesion of microorganisms to a denture surface is a prerequisite for colonization.
  
  
  OBJECTIVE
  This study compared the retention of Candida albicans on smooth and rough acrylic resin and silicone surfaces after a washing procedure to determine the effect of surface roughness on prosthesis infection and hygiene.
  
  
  METHODS
  Standardized cell suspensions of C. albicans were incubated with smooth and rough acrylic resin and silicone surfaces for 1 hour at 24 degrees C. After washing, cells that had been retained on the surface were stained with acridine orange and examined with incident beam fluorescent microscopy.
  
  
  RESULTS
  There was no significant difference in cell numbers on either of the smooth surfaces. Significantly higher numbers of cells (p > 0.0005) were observed on roughened surfaces (silicone > acrylic resin) than on smooth surfaces. The fitting surface of the maxillary denture was not polished.
  
  
  CONCLUSIONS
  Silicones used in prostheses were processed against dental stone. The resultant surface roughness may facilitate microbial retention and infection and should therefore be kept to a minimum.
authors.affiliation: Department of Biological Sciences, Manchester Metropolitan University, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: Verran
authors.forename: C J
authors.initials: CJ
authors.lastname: Maryan
mesh_terms: D000165:Acridine Orange; D000180:Acrylic Resins / Q000737:chemistry; D002176:Candida albicans / Q000166:cytology / Q000502:physiology*; D004396:Coloring Agents; D003819:Denture Bases; D003778:Dentures; D006801:Humans; D008856:Microscopy, Fluorescence; D009910:Oral Hygiene; D016459:Prosthesis-Related Infections / Q000382:microbiology; D012828:Silicones / Q000737:chemistry; D013499:Surface Properties; D007507:Therapeutic Irrigation
chemical_list: D000180:Acrylic Resins; D004396:Coloring Agents; D012828:Silicones; D000165:Acridine Orange