The calcium-binding protein VILIP in olfactory neurons: regulation of second messenger signaling.
Visinin-like-protein (VILIP), a member of the neuronal subfamily of EF-hand calcium-sensor proteins is shown to be expressed in olfactory sensory cells of the rat nasal epithelium. Its prominent localization in cilia and dendritic knobs-the chemosensory compartments of olfactory neurons-suggests that the calcium-binding protein could be involved in olfactory signal transduction. Consistent with this assumption, it was found that recombinant VILIP attenuates in a calcium-dependent manner odorant-induced cAMP formation in olfactory cilia preparations. Kinetic data indicate that VILIP does not interfere with odorant-induced receptor desensitization. Since VILIP inhibits the forskolin-induced formation of cAMP, it is conceivable that VILIP may directly affect the olfactory adenylyl cyclase. Thus, VILIP may play a role in adaptation of olfactory neurons.
Boekhoff
I
I
Institute of Zoophysiology, University of Hohenheim, Stuttgart/Germany.
Braunewell
K H
KH
Andreini
I
I
Breer
H
H
Gundelfinger
E
E
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000378:metabolism* / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D007150:Immunohistochemistry; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000494:pharmacology; D051596:Neurocalcin; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044169:Receptors, Calcium-Sensing; D011994:Recombinant Proteins / Q000494:pharmacology; D015290:Second Messenger Systems / Q000187:drug effects / Q000502:physiology*
D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D044169:Receptors, Calcium-Sensing; D011994:Recombinant Proteins; C496690:Vsnl1 protein, rat; D005576:Colforsin; D000242:Cyclic AMP; D002118:Calcium