Signal transduction through atypical PKCs, but not the EGF receptor, is necessary for UVC-induced AP-1 activation in immortal murine cells.
The exposure of mammalian cells to ultraviolet (u.v.) irradiation leads to activation of transcription factors, such as AP-1 and NFkappaB. It is postulated that the EGF receptor but not protein kinase C (PKC) is the major membrane mediator in UVC-induced signal transduction. We demonstrate here that the antisense oligonucleotides of PKC zeta and the dominant negative mutant of PKC lambda/iota as well as dominant negative PKC zeta markedly blocked UVC-induced AP-1 activity. In contrast, UVC-induced AP-1 activity in cells devoid of the EGF receptor (B82), is not significantly different from that of the stable transfectants with a kinase-deficient EGF receptor (B82M721), or wild-type EGF receptor (B82L). This was found at all UVC irradiation doses and time courses studied, while high levels of EGF-induced AP-1 activity were observed in B82L cells but not in B82 cells. This evidence strongly suggests that atypical PKCs, but not the EGF receptor, is necessary for UVC-induced AP-1 activation in JB6 and B82 cells.
Huang
C
C
The Hormel Institute, University of Minnesota, Austin 55912, USA.
Ma
W
W
Dong
Z
Z
D000818:Animals; D002461:Cell Line, Transformed; D066246:ErbB Receptors / Q000096:biosynthesis / Q000502:physiology*; D005347:Fibroblasts; D017930:Genes, Reporter; D007527:Isoenzymes; D007700:Kinetics; D008156:Luciferases / Q000096:biosynthesis; D051379:Mice; D011493:Protein Kinase C / Q000096:biosynthesis / Q000378:metabolism*; D015398:Signal Transduction; D012867:Skin; D018808:Transcription Factor AP-1 / Q000378:metabolism / Q000528:radiation effects*; D014162:Transfection; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000096:biosynthesis; D000099293:Protein Kinase C-lambda; D000099221:Protein Kinase C zeta
D066246:ErbB Receptors; D007527:Isoenzymes; D008156:Luciferases; D011493:Protein Kinase C; D018808:Transcription Factor AP-1; D001616:beta-Galactosidase; D000099293:Protein Kinase C-lambda; D000099221:Protein Kinase C zeta