Immunoregulatory effect of substance P in human eosinophil migratory function.
Substance P (SP) is a tachykinin involved in the regulation of inflammatory processes. To investigate a modulatory role of the neuropeptide SP in allergic inflammation, we studied its priming effect on human eosinophil chemotaxis and kinetic responses towards platelet activating factor (PAF) and recombinant human interleukin 5 (rhIL-5). Blood was obtained from normal subjects and eosinophils were separated by Percoll discontinuous density gradient centrifugation. High purification was obtained by negative selection procedure (CD16-beads) and the experiments were performed in a 48-well microchemotaxis Boyden chamber. In the present study we demonstrate a potent synergistic effect of 1OOnM dose of SP on the migratory function of human eosinophils stimulated by PAF and rhIL- 5. This synergism was chemotaxis specific and was abolished by NK-1 receptor antagonist (FK888). The results suggest that neurogenic stimuli may play a significant role in eosinophil infiltration via its priming effect on the cell.
El-Shazly
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AE
Department of Otorhinolaryngology, School of Medicine, Kumamoto University, Japan.
Masuyama
K
K
Eura
M
M
Ishikawa
T
T
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000276:immunology; D004151:Dipeptides / Q000494:pharmacology; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology* / Q000502:physiology; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D007249:Inflammation / Q000209:etiology; D015848:Interleukin-5 / Q000494:pharmacology; D008297:Male; D064729:Neurokinin-1 Receptor Antagonists; D010972:Platelet Activating Factor / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D013373:Substance P / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D004151:Dipeptides; D007211:Indoles; D015848:Interleukin-5; D064729:Neurokinin-1 Receptor Antagonists; D010972:Platelet Activating Factor; D011994:Recombinant Proteins; C078949:FK 888; D013373:Substance P