High salt and solvent induced Z-conformation in native calf thymus DNA.
The Z-DNA forming potentiality of native calf thymus DNA using spectrophotometric measurements and diethyl pyrocarbonate reactivity have been investigated. The Z value was evaluated from absorbance ratio for B- and Z-DNA and compared with the values obtained for native DNA. The results suggest that native DNA undergoes B- to Z-/Z-analogous transition to an extent of 20 per cent under constraint conditions. Possible Z-DNA formation was also characterized by hyper-reactivity of native DNA towards diethyl pyrocarbonate in aqueous solvent. The thermal melting profile of native and modified DNA and nuclease S1 digestibility data, in addition, reflects B- to Z-/Z-analogous transition in native DNA.
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Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
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D000818:Animals; D002417:Cattle; D004247:DNA / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D004047:Diethyl Pyrocarbonate / Q000378:metabolism / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009691:Nucleic Acid Denaturation / Q000187:drug effects / Q000502:physiology; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013950:Thymus Gland / Q000737:chemistry
D012997:Solvents; D012965:Sodium Chloride; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases; D004047:Diethyl Pyrocarbonate