[Rheologic study of the dissociation reaction of erythrocyte immunologic agglutinates: application to subgroup A erythrocytes].
Red blood cell Agglutinates can be dissociated by providing enough energy which may be supplied by shear stress in a viscometer. In this paper a new technique is proposed to determine dissociation parameters of erythrocyte immunological agglutinates by using a laser backscattering method. Backscattered light intensity increases during the process of agglutinate dissociation when a controlled shear stress in a transparent Couette viscometer is applied. The obtained curves of dissociation can be fitted by an exponential function. A numerical integration of the dissociation curves allows us to obtain a parameter associated to the energy used in the dissociation process. A satisfactory differentiation of erythrocyte sub-groups Al, Az, A,B and Am has been carried out using this technique.
Rasia-Valverde
J R
JR
Laboratorio de Immunohematología, Hemorreología e Histocompatibilidad, Fac. de Cs. Bioquim. y Farmac., Rosario, Argentine.
Rasia
R J
RJ
Botte
C
C
Stoltz
J F
JF
D001809:Blood Viscosity; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D007834:Lasers; D037102:Lectins; D016015:Logistic Models; D015203:Reproducibility of Results; D012212:Rheology; D012542:Scattering, Radiation; D013314:Stress, Mechanical
D037102:Lectins
Approche rhéologique de la réaction de dissociation d'agglutinats immunologiques érythrocytaires: application à des hématies du sous-groupe A.