A novel method for isolation of Chlamydia pneumoniae by treatment with trypsin or EDTA.
To establish a novel method for the efficient isolation of Chlamydia pneumoniae, experiments were performed to determine the effects of EDTA or trypsin treatment of C. pneumoniae on its adsorption and inclusion body formation. Treatment of C. pneumoniae with 0.1% trypsin or 1 mM EDTA significantly increased inclusion body-forming activity from 8,000- to 10,000-fold higher than that of the control. C. pneumoniae was successfully isolated in cultured cells which were inoculated with clinical specimens after treatment with 0.1% trypsin.
Kazuyama
Y
Y
Research and Development Center of Hygienic Science, Kitasato University, Minatoku, Tokyo, Japan.
Lee
S M
SM
Amamiya
K
K
Taguchi
F
F
D001431:Bacteriological Techniques; D002460:Cell Line; D002614:Chelating Agents / Q000494:pharmacology; D016993:Chlamydophila pneumoniae / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004432:Ear, Middle / Q000382:microbiology; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D002479:Inclusion Bodies / Q000187:drug effects; D008168:Lung; D010614:Pharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D013183:Sputum / Q000382:microbiology; D014357:Trypsin / Q000494:pharmacology
D002614:Chelating Agents; D004269:DNA, Bacterial; D004492:Edetic Acid; D014357:Trypsin