title: Use of enzyme-linked immunosorbent assay techniques with cross-reacting human sera in diagnosis of murine typhus and spotted fever.
abstract: Enzyme-linked immunosorbent assay (ELISA) techniques for the determination of immunoglobulin G to rickettsial lipopolysaccharides were developed. These techniques provide a simple and convenient way to serodiagnose Mediterranean spotted fever and murine typhus with a single serum dilution. The results of the ELISAs correlated with the indirect immunofluorescence assay titers of cross-reacting sera.
authors.affiliation: Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Keysary
authors.forename: C
authors.initials: C
authors.lastname: Strenger
mesh_terms: D000818:Animals; D001907:Boutonneuse Fever / Q000175:diagnosis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D012281:Rickettsia / Q000276:immunology / Q000302:isolation & purification*; D014437:Typhus, Endemic Flea-Borne / Q000175:diagnosis*