Receptor-induced beta gamma release from fatty acylation-deficient mutants of G alpha z.
The neuronal-specific G protein Gz is known to interact with a large variety of receptors for neurotransmitters and hormones. Fatty acylations on the N-terminus of the alpha subunit of Gz (alpha z) provide anchorage to the plasma membrane. Fatty acylation-deficient mutants of alpha z have previously been shown to exhibit altered signaling properties. Since the N-terminus of alpha z is likely to play a critical role in beta gamma binding, we examined the ability of these mutants to interact with beta gamma subunits by means of receptor-mediated stimulation of beta gamma-sensitive type II adenylyl cyclase. Our results indicate that lack of myristoylation, but not lack of palmitoylation, impaired the ability of alpha z to mediate receptor-induced release of beta gamma subunits.
Beck
H I
HI
Department of Biology, Hong Kong University of Science and Technology, Kowloon, Hong Kong.
Chan
J S
JS
Wong
Y H
YH
D000215:Acylation; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D000242:Cyclic AMP / Q000378:metabolism; D018076:DNA, Complementary; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D046911:Macromolecular Substances; D016297:Mutagenesis, Site-Directed; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D017354:Point Mutation; D017465:Receptors, Opioid, delta / Q000096:biosynthesis / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D018076:DNA, Complementary; D004745:Enkephalins; D046911:Macromolecular Substances; D009227:Myristic Acids; D017465:Receptors, Opioid, delta; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; D019814:Myristic Acid; D019308:Palmitic Acid; D020881:Enkephalin, D-Penicillamine (2,5)-; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases