Diminishing adenovirus gene expression and viral replication by promoter replacement.
The adenovirus E4 promoter was replaced by a synthetic promoter composed of a minimal TATA box and five consensus 17-mer yeast GAL4-binding-site elements. The viral vectors, which also contained human factor IX (hFIX) cDNA driven by Rous sarcoma virus long terminal repeat in the E1 region, were then constructed and expanded in 293 cells permanently expressing GAL4/VP16 fusion protein. Viral replication and expression of adenovirus E4 genes and late genes (hexon and fiber) were evaluated in vitro in the human lung carcinoma cell line H1299. Viral replication and viral gene expression were dramatically reduced in the cells transduced by vectors with a replaced E4 promoter compared to the levels in the cells transduced by vectors with the wild-type E4 promoter. The levels of transgene (hFIX) expression remained similar between vectors with or without E4 promoter replacement. These results indicate that diminution of viral gene expression and viral replication is achievable by promoter replacement.
Fang
B
B
Department of Thoracic and Cardiovascular Surgery, The University of Texas M. D. Anderson Cancer Center, Houston 77030, USA. bfang@surg.mdacc.tmc.edu
Koch
P
P
Roth
J A
JA
D000256:Adenoviridae / Q000235:genetics*; D002213:Capsid / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins; D002460:Cell Line; D004268:DNA-Binding Proteins; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006801:Humans; D011401:Promoter Regions, Genetic; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D036022:Capsid Proteins; D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C018315:hexon capsid protein, Adenovirus