title: Immunophenotypic analysis of non-Hodgkin's lymphoma.
abstract: One hundred and three cases of Non-Hodgkin's lymphoma were evaluated immunohistochemically using a panel of monoclonal antibodies which includes leucocyte common antigen (LCA), CD45R (Pan-B marker), L-26 (CD 20-Pan-B marker) and UCHL-1 (Pan-T marker). Of the total 63 cases (61.17%) showed a B-cell phenotype while 40 (38.83%) were of T-cell origin. Most B-cell neoplasms belonged to intermediate (79.36%) or high grade (15.87%) according to the International Working Formulation (WF). Most T-cell lymphomas were of either intermediate (52.5%) or high grade (32.5%) neoplasms. Some T-cell neoplasms presented as specific clinicopathological entities like lymphomatoid granulomatosis (2 cases), mycosis fungoides (1 case) and AILD type NHL (1 case). In 27 cases the immunostaining pattern of two. Pan-B markers i.e., L26 and CD45R was compared. L26 staining was expressed in all 27 cases (100% sensitivity) while CD45R showed positive reaction in 22 cases (82% sensitivity). UCHL-1 is proved to be a sensitive and lineage specific T-cell marker and in 67% cases the staining pattern was moderate (+2) to intense (+3). The mean age for the B-cell lymphomas was 49 years and 36 years in T-cell neoplasm. Male to female ratio in both types of lymphomas was 2:1. The study indicates a high prevalence of T-cell when comparing the data from western countries and lower to those from Japan and Caribbean countries.
authors.affiliation: Department of Pathology, Aga Khan University Hospital, Karachi.
authors.forename: S
authors.initials: S
authors.lastname: Muzaffar
authors.forename: S
authors.initials: S
authors.lastname: Pervez
authors.forename: F
authors.initials: F
authors.lastname: Aijaz
authors.forename: S A
authors.initials: SA
authors.lastname: Aziz
authors.forename: S H
authors.initials: SH
authors.lastname: Hasan
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000187:drug effects; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000145:classification / Q000276:immunology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000145:classification* / Q000276:immunology / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000145:classification / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: D000911:Antibodies, Monoclonal