beta-Glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitidis.
Wild-type cells of the pathogenic black yeast Exophiala (Wangiella) dermatitidis grown in a low-pH ascorbate medium became less melanized and less resistant to Zymolyase. This was accompanied by increased staining with fluorescently labelled concanavalin A. The sugar composition of wild-type and mutant cell walls was, except for the presence of galactose, similar to that of Saccharomyces cerevisiae. Digestion of mutant cell walls with laminarinase released galactomannoproteins. In addition, the released cell wall proteins contained glucose and reacted with affinity-purified 1,6-beta-glucan antiserum, indicating that they are linked to 1,6-beta-glucan. It is proposed that 1,6-beta-glucosylated cell wall proteins generally occur among ascomycetes.
Montijn
Roy C
RC
Institute of Molecular Cell Biology, University of Amsterdam, Biocentrum Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
Van Wolven
Patricia
P
Institute of Molecular Cell Biology, University of Amsterdam, Biocentrum Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
De Hoog
Sybren
S
Centraalbureau voor Schimmelcultures, PO Box 273, Baarn, The Netherlands.
Klis
Frans M
FM
Institute of Molecular Cell Biology, University of Amsterdam, Biocentrum Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000737:chemistry*; D003470:Culture Media; D005093:Exophiala / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D005690:Galactose / Q000032:analysis; D005936:Glucans / Q000302:isolation & purification; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D008358:Mannose / Q000032:analysis; D008543:Melanins / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000302:isolation & purification
D003470:Culture Media; D005656:Fungal Proteins; D005936:Glucans; D006023:Glycoproteins; D008543:Melanins; D008562:Membrane Glycoproteins; C017336:galactoproteins; C025363:mannoproteins; D008358:Mannose; D005690:Galactose