Growth ability of auxiliary gene mutants of human immunodeficiency virus types 1 and 2 in unstimulated peripheral blood mononuclear cells.
Mutational studies on the Vif, Vpr, Vpu, Vpx, and Nef genes of human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) were performed to evaluate their biological functions in natural target cells. For this purpose, replication properties of mutant viruses derived from HIV-1 NL strain and HIV-2 GH strain in unstimulated peripheral blood mononuclear cells were determined. Vif- viruses of both HIV-1 and HIV-2 did not grow at all in these cells. Similarly, no replication of HIV-2 Vpx- mutant was detected. In contrast, both of Vpr- and Nef- viruses of HIV-1 and HIV-2, and Vpu- virus of HIV-1 grew quite well in the cells. These results show, together with the data previously reported, that only Vif and Vpx are essential for HIV replication in primary blood cell cultures.
Tokunaga
K
K
Institute for Virus Research, Kyoto University, Japan.
Ishimoto
A
A
Ikuta
K
K
Adachi
A
A
D002478:Cells, Cultured; D005814:Genes, Viral; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D015498:HIV-2 / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D009154:Mutation; D014779:Virus Replication / Q000235:genetics*