Root colonization of different plants by plant-growth-promoting Rhizobium leguminosarum bv. trifolii R39 studied with monospecific polyclonal antisera.
Monospecific polyclonal antisera raised against Rhizobium leguminosarum bv. trifolii R39, a bacterium which was isolated originally from red clover nodules, were used to study the colonization of roots of leguminous and nonleguminous plants (Pisum sativum, Lupinus albus, Triticúm aestivum, and Zea mays) after inoculation. Eight weeks after inoculation of soil-grown plants, between 0.1 and 1% of the total bacterial population in the rhizospheres of all inoculated plants were identified as R. leguminosarum bv. trifolii R39. To characterize the associative colonization of the nonleguminous plants by R.leguminosarum bv. trifolii R39 in more detail, a time course study was performed with inoculated roots of Z. mays. R. leguminosarum bv. trifolii R39 was found almost exclusively in the rhizosphere soil and on the rhizoplane 4 weeks after inoculation. Colonization of inner root tissues was detected only occasionally at this time. During the process of attachment of R. leguminosarum bv. trifolii R39 to the rhizoplane, bacterial lipopolysaccharides were overexpressed, and this may be important for plant-microbe interaction. Fourteen weeks after inoculation, microcolonies of R. leguminosarum bv. trifolii R39 were detected in lysed cells of the root cortex as well as in intracellular space of central root cylinder cells. At the beginning of flowering (18 weeks after inoculation), the number of R. leguminosarum bv. trifolii R39 organisms decreased in the rhizosphere soil, rhizoplane, and inner root tissue.
Schloter
M
M
Institute of Soil Ecology, GSF-National Research Center for Environment and Health, Neuherberg, Germany. schloter@gsf.de
Wiehe
W
W
Assmus
B
B
Steindl
H
H
Becke
H
H
Höflich
G
G
Hartmann
A
A
D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D015169:Colony Count, Microbial; D003429:Cross Reactions / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D007887:Fabaceae / Q000382:microbiology; D019084:Fluorescent Antibody Technique, Indirect; D007118:Immunoassay; D008070:Lipopolysaccharides / Q000378:metabolism; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D018517:Plant Roots / Q000382:microbiology*; D010944:Plants / Q000382:microbiology; D010946:Plants, Medicinal; D016961:Rhizobium leguminosarum / Q000254:growth & development* / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors; D003313:Zea mays / Q000382:microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D008070:Lipopolysaccharides