mRNA stability and localisation of the low-temperature-responsive barley gene family blt14.
Transcription and translation inhibitors have been used to investigate the role of mRNA stability in the low-temperature-regulated expression of the post-transcriptionally controlled low temperature responsive barley gene family, blt14. Genomic clones (blt14.1, blt14.2) representing additional members of the blt14 gene family have been isolated and sequenced. Gene specific probes have been used to analyse the spatial expression of each individual member of the blt14 gene family. Findings indicate that all of the genes are responsive to low temperature, but the organ distribution is different for each gene. The results indicate that blt14.0 mRNA is stabilised by a low-temperature-dependent protein factor. Taken together, the results suggest that organ-specific post-transcriptional mechanisms are important in the low-temperature regulation of blt14 gene expression.
Phillips
J R
JR
Department of Biochemistry and Genetics, University of Newcastle upon Tyne, UK.
Dunn
M A
MA
Hughes
M A
MA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D003513:Cycloheximide / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009153:Mutagens / Q000494:pharmacology; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D018749:RNA, Plant / Q000032:analysis / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D003839:Deoxyadenosines; D006360:Heat-Shock Proteins; D009153:Mutagens; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D018749:RNA, Plant; C106213:blt14 protein, Hordeum vulgare; D003513:Cycloheximide; C058120:cordycepin