An equine herpesvirus-1 gene 71 deletant is attenuated and elicits a protective immune response in mice.
The pathogenesis of pulmonary infection and the immune response following intranasal inoculation of mice with two equine herpesvirus type 1 (EHV-1) deletion mutants have been assessed. The mutants, ED71 and ED75, have deletions in genes 71 (EUS4) and 75 (10K), respectively. Deletions were replaced by the Escherichia coli lacZ gene driven by the simian virus 40 (SV40) early promoter. It has previously been shown that the protein products of genes 71 and 75 are dispensable in vitro but that removal of gene 71 results in a defect in virus maturation and capsid envelopment which impairs the ability of mutant virus to spread via release and readsorption. This study demonstrated that the 192-kDa gene 71 product is required for full expression of virulence in mice, whereas the putative 10-kDa product of gene 75 has minimal effect. Both mutants exhibited the same tissue and cytotropism as wild-type EHV-1 and induced both humoral and cell-mediated immune responses indistinguishable from those induced by the parental strain. Irrespective of the reduced pathogenicity of the gene 71 mutant, infected mice were protected against a challenge with wild-type EHV-1. These findings highlight the potential of ED71 as a vaccine candidate.
Marshall
K R
KR
MRC Virology Unit, Glasgow, United Kingdom.
Sun
Y
Y
Brown
S M
SM
Field
H J
HJ
D000818:Animals; D005260:Female; D017353:Gene Deletion; D005814:Genes, Viral; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control*; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D051379:Mice; D008807:Mice, Inbred BALB C; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D014613:Vaccines, Attenuated; D014765:Viral Vaccines