[Nucleotide sequence of cDNA and organization of the gene for alpha-subunit of photoreceptor phosphodiesterase of cyclic GMP in human retinal cones].
Five clones were isolated from a human retina cDNA library whose cDNA inserts allowed reconstruction of the total sequence of the human clone cGMP phosphodiesterase alpha'-subunit cDNA comprising 3455 bp. The protein's deduced sequence contains 858 amino acids residues with molecular mass 99,169 Da. A substantial homology was revealed between the amino acid sequence of the human cones cGMP-phosphodiesterase alpha'-subunit and the corresponding sequences of alpha, beta, and alpha' subunits of visual cGMP-phosphodiesterase of bovine, murine, chicken and human retinas. Four recombinant bacteriophages were isolated from a genomic library whose inserts made it possible to reconstruct a 32-kb fragment of the human cones cGMP-phosphodiesterase alpha'-subunit gene. 5'-Flanking region of the gene and first 14 exons, encoding an N-terminal segment of the protein, along with the adjacent intron segments were sequenced.
Feshchenko
E A
EA
Andreeva
S G
SG
Suslova
V A
VA
Rakitina
T V
TV
Smirnova
E V
EV
Bystrov
N S
NS
Zagranichnyĭ
V E
VE
Lipkin
V M
VM
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D017949:Retinal Cone Photoreceptor Cells / Q000201:enzymology*
D018076:DNA, Complementary; D015106:3',5'-Cyclic-GMP Phosphodiesterases
Nukleotidnaia posledovatel'nost' kDNK i organizatsiia gena alpha'-sub''edinitsy fotoretseptornoĭ fosfodiésterazy tsiklicheskogo GMP kolbochek setchatki glaza cheloveka.