Recognition of osteopontin by rat bone marrow derived osteoblastic primary cells.
To study the role of osteopontin, we did cell adhesion and ALP assays of rat bone marrow osteoblastic cells (RBMO) on collagen Type I and osteopontin surfaces. The RBMO proved to adhere much more strongly to the osteopontin and to have higher ALP activity on the osteopontin, which suggests that pre-osteoblasts differentiate into osteoblasts that form bone by recognizing osteopontins.
Yabe
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T
Bionic Design Group, National Institute for Advanced Interdisciplinary Research (NAIR), Tsukuba Research Center, Ibaraki, Japan.
Nemoto
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A
Uemura
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T
D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001853:Bone Marrow / Q000201:enzymology; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002454:Cell Differentiation; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D053495:Osteopontin; D051381:Rats; D012795:Sialoglycoproteins / Q000502:physiology*
D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; D000469:Alkaline Phosphatase