Changes in lymphocyte subsets following administration of interleukin 2 and cyclophosphamide in mice with transitional cell carcinoma.
We investigated the effects of combination therapy with interleukin 2(IL-2) and cyclophosphamide (CPM) in C3H/HeN mice implanted with mouse bladder tumor cells (MBT2). Tumor growth was suppressed in the mice treated with IL-2 alone and mice treated with CPM alone, although not completely. However, tumor growth was completely suppressed in the mice treated with both IL-2 and CPM. As for lymphocyte subset analysis of the spleen using flow cytometry, the frequency of Lyt2+ cells and asialo GM1+ cells was significantly higher in the mice treated with IL-2 and CPM compared to the other groups. In the lymphocyte subset analysis of the thymus, the frequency of L3T4-Lyt2+ cells was significantly lower in the mice treated with CPM alone and mice treated with IL-2 and CPM compared to the other groups.
Ikemoto
S
S
Department of Urology, Osaka City University Medical School, Japan.
Kamizuru
M
M
Wada
S
S
Asai
Y
Y
Kishimoto
T
T
D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D003520:Cyclophosphamide / Q000627:therapeutic use*; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000627:therapeutic use*; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009929:Organ Size; D011994:Recombinant Proteins / Q000627:therapeutic use; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013997:Time Factors; D001749:Urinary Bladder Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*
D007376:Interleukin-2; D011994:Recombinant Proteins; D003520:Cyclophosphamide