The S.//.A.IG amino acid motif is present in a replication dependent late H3 histone variant of P. lividus sea urchin.
A novel gene encoding a new H3 histone varian (H3L) has been identified in P. lividus sea urchin embryo. It encodes a H3 histone protein showing the S.//.A.IG amino acid motif typical of the replication independent H3.3 variants but in a mRNA showing the 3' terminal stem-loop nucleotide sequence that is typical of the replication dependent variants. The gene is intronless, the corresponding short transcript is non-polyadenyl ated and its expression is replication dependent with a timing of late variant. The new H3 variant is expressed as a minor component with respect to a major replication dependent late H3 histone here identified by partial cDNA sequence. These results show that classification of histones in replication dependent and independent variants only on the basis of their amino acid sequences should be reconsidered.
Fucci
L
L
Department of Genetics, General and Molecular Biology, University of Naples Federico II, Italy. fucci@dgbm.unina.it
Forte
A
A
Mancini
P
P
Affaitati
A
A
Branno
M
M
Aniello
F
F
Geraci
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004261:DNA Replication; D047108:Embryonic Development; D014644:Genetic Variation; D015698:Genomic Library; D006657:Histones / Q000235:genetics*; D008969:Molecular Sequence Data; D012617:Sea Urchins / Q000196:embryology / Q000235:genetics*; D017422:Sequence Analysis, DNA
D006657:Histones