title: Artificial insemination and in-vitro fertilization using donor spermatozoa: a report on 15 years of experience.
abstract: Donor insemination (DI) using cryopreserved semen commenced at The Royal Women's Hospital in 1976. Over the next 15 years we performed 5953 treatment cycles to achieve 816 pregnancies (13.7% per cycle) and 706 live births. In-vitro fertilization (IVF) using donor spermatozoa commenced in 1986. Over the next 5 years we performed 303 treatment cycles for 185 couples. Including subsequent transfer of cryopreserved embryos, a total of 33% of couples achieved a successful pregnancy by IVF. Statistical analysis indicated that, for DI pregnancies, the most important semen variable was the percentage post-thaw motility, whilst for normal fertilization in IVF it was the pre-freeze motility. These results may be explained by the compensatory effects of post-thaw processing of spermatozoa for IVF, but not for DI in our clinic.
authors.affiliation: The Royal Women's Hospital, Melbourne, Australia.
authors.forename: G N
authors.initials: GN
authors.lastname: Clarke
authors.forename: H
authors.initials: H
authors.lastname: Bourne
authors.forename: P
authors.initials: P
authors.lastname: Hill
authors.forename: W I
authors.initials: WI
authors.lastname: Johnston
authors.forename: A
authors.initials: A
authors.lastname: Speirs
authors.forename: J C
authors.initials: JC
authors.lastname: McBain
authors.forename: H W
authors.initials: HW
authors.lastname: Baker
mesh_terms: D000293:Adolescent; D000328:Adult; D001315:Australia; D015925:Cryopreservation; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007316:Insemination, Artificial, Heterologous; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D013081:Sperm Motility; D016896:Treatment Outcome