[A new PCR-primer for specific amplification of human DNA fragments selected on the basis of computer analysis of the nucleotide sequences of MER1 dispersed repeats in man].
The possibility of using oligonucleotides from MER1 family repeats as PCR primers for the amplification of human genome DNA fragments was studied. The recommended oligonucleotide primers were chosen by computer analysis of the data base of the EMBL (release 37.0) nucleotide sequences. Use of one of the oligonucleotides was shown to allow specific human DNA amplification to be carried out.
Iantsen
E I
EI
Ptitsyn
A A
AA
Filipenko
M L
ML
Batyrina
O A
OA
Muravlev
A I
AI
Mertvetsov
N P
NP
D001483:Base Sequence; D053938:DNA Fragmentation; D017931:DNA Primers; D016208:Databases, Factual; D005787:Gene Frequency; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D012984:Software
D017931:DNA Primers
Novyĭ PTsR-praĭmer dlia spetsifichnoĭ amplifikatsii fragmentov DNK cheloveka, vybrannyĭ na osnove komp'iuternogo analiza nukleotidnykh posledovatel'nosteĭ dispergirovannykh povtorov cheloveka MER1.