title: [Chemical tests with Marrubium species. Official data on Marubii herba in Pharmacopoeia Hungarica VII].
abstract: About 40 species of the Marrubium genus (Lamiaceae) are known of which 2 species (M. vulgare L. and M. peregrinum L.) and one hybrid (M. x paniculatum Desr.) can be found as native plants in Hungary. The above-ground parts of M. vulgare L. are official in Hungarian Pharmacopoeia VII. Active substances in Marrubii herba are labdane-structured bitter materials. Although the presence of furanic labdane diterpenes in the plant is known, the pharmacopoeia gives only microscopic tests, qualitative tests (for other parts of the plant and foreign organic matter) for the bitter value of Marrubii herba. We have examined the main terpenoid substances isolated with column, gel and preparative layer chromatography. Structure elucidations were performed by means of UV, mass and NMR spectroscopy. We have compared the changes in terpenoid-type compounds (premarrubiin and marrubiin) in plants during the vegetation period; in different Marrubium species and in the different extractions of horehound by means of thin layer chromatography and densitometry. By reason of our results we propose qualitative and quantitative chemical tests for the paragraph of Marrubii herba in Pharmacopoeia Hungarica VII.
authors.affiliation: Jókai Pharma Gyógyszerkereskedelmi Betéti Társaság, Hódmezóvásárhely.
authors.forename: E
authors.initials: E
authors.lastname: Telek
authors.forename: L
authors.initials: L
authors.lastname: Tõth
authors.forename: L
authors.initials: L
authors.lastname: Botz
authors.forename: I
authors.initials: I
authors.lastname: Máthé
mesh_terms: D004224:Diterpenes; D006814:Hungary; D015394:Molecular Structure; D010602:Pharmacopoeias as Topic; D010946:Plants, Medicinal; D013045:Species Specificity; D013729:Terpenes
chemical_list: D004224:Diterpenes; D013729:Terpenes; C105732:premarrubiin; C105731:marrubiin
vernacular_title: Marrubium fajok növénykémiai vizsgálata Adatok a VII. Magyar Gyógyszerkönyv Marrubii herba cikkelyéhez.