Functional and ultrastructural studies on the burst of c-kit ligand containing secretory cytoplasmatic vesicles.
A fibroblast-like cell line (D064) was established from canine marrow long-term cultures. These cells start to differentiate into hematopoietic progenitor precursors under the influence of c-kit ligand, functioning in an autocrine and paracrine fashion. The message for c-kit ligand is transcribed in the fibroblast-like cell line and the ligand for the tyrosine-kinase c-kit is expressed on the cell surface as well as secreted in its soluble form. With a monoclonal antibody directed against all isoforms of c-kit ligand, the expression of c-kit ligand was investigated functionally and topographically by electron microscopy. Ultrastructural analysis revealed c-kit ligand in electron dense vesicles which were transported to the cell surface and burst to release the soluble, non-membrane bound isoform of c-kit ligand. A membrane bound isoform of c-kit ligand was also present on the cell membrane.
Huss
R
R
Institute of Pathology, University of Munich, Germany.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000648:ultrastructure*; D004285:Dogs; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000648:ultrastructure; D008854:Microscopy, Electron; D019089:Stem Cell Factor / Q000276:immunology / Q000378:metabolism* / Q000502:physiology
D019089:Stem Cell Factor