Antisense oligodeoxynucleotide to delta opioid receptors blocks cocaine-induced place preference in mice.
The effect of intracerebroventricular (i.c.v.) treatment with antisense oligodeoxynucleotide (A-oligo) to delta opioid receptor mRNA on cocaine-induced place preference was examined in mice. Cocaine (10 mg/kg, s.c.) produced a significant place preference. I.c.v. treatment with A-oligo (0.001-1 microg/mouse) dose-dependently attenuated the cocaine (10 mg/kg, s.c.)-induced place preference, although mismatched oligodeoxynucleotide (1 microg/mouse, i.c.v.) was ineffective. In the present study, we found that the selective reduction in number and/or function of central delta opioid receptors by A-oligo suppresses the cocaine-induced place preference. These results suggest that the conditioned reward by cocaine may be partially mediated by central delta opioid receptors.
Suzuki
T
T
Department of Pharmacology, School of Pharmacy, Hoshi University, Shinagawa-ku, Tokyo, Japan.
Tsuji
M
M
Ikeda
H
H
Misawa
M
M
Narita
M
M
Tseng
L F
LF
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D003042:Cocaine / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D003214:Conditioning, Classical / Q000187:drug effects*; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009292:Narcotic Antagonists / Q000494:pharmacology*; D009294:Narcotics / Q000494:pharmacology*; D009619:Nociceptors / Q000187:drug effects; D009841:Oligonucleotides / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D017465:Receptors, Opioid, delta / Q000819:agonists / Q000235:genetics* / Q000502:physiology
D009292:Narcotic Antagonists; D009294:Narcotics; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D017465:Receptors, Opioid, delta; D003042:Cocaine