In vitro effect of natural and semi-synthetic carbohydrate polymers on Chlamydia trachomatis infection.
The effect of different natural and semi-synthetic polysaccharides on Chlamydia trachomatis multiplication in Hela 229 cells was evaluated. Some neutral, negatively and positively charged carbohydrates were able, in a dose-dependent fashion, to inhibit chlamydial infection by interfering mainly with the adsorption process. The inhibiting compounds, whose effect was shown within the concentration range of 8-200 micrograms/ml, were in order of action: dextran sulphate > glyloid sulphate 4327 > glycogen sulphate 4427 > arabic gum = glyloid > chitosan > glycogen. Data obtained suggested that antichlamydial activity was not only related to the electric charge of these molecules but could also be attributed to other features of their polymeric backbone. Since carbohydrate polymers have also been shown to inhibit the early stages of infection by viral agents causing sexually transmitted diseases, the employment of these molecules for prevention or treatment of mixed viral-C. trachomatis infections can be hypothesized.
Petronio
M G
MG
Microbiology Institute, School of Medicine, University La Sapienza, Rome, Italy.
Mansi
A
A
Gallinelli
C
C
Pisani
S
S
Seganti
L
L
Chiarini
F
F
D002686:Chitin / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D048271:Chitosan; D002690:Chlamydia Infections / Q000188:drug therapy*; D002692:Chlamydia trachomatis / Q000187:drug effects*; D006003:Glycogen / Q000494:pharmacology / Q000627:therapeutic use; D006367:HeLa Cells; D006801:Humans; D011134:Polysaccharides / Q000494:pharmacology* / Q000627:therapeutic use
D011134:Polysaccharides; D002686:Chitin; C018558:glyloid; D006003:Glycogen; D048271:Chitosan