title: Decrease in expression of the master operon of flagellin synthesis in a dnaA46 mutant of Escherichia coli.
abstract: We examined the expression of the fliC, fliA and fihD genes in the dnaA46 mutant. In Northern blot analysis, the amounts of fliC, fliA, and flhD mRNA decreased in the dnaA46 mutant growing at 37 degrees C but not at 28 degrees C. The promoter activity of each gene also decreased in this mutant at 37 degrees C. The decrease in expression of the flh D gene was not related to the cAMP-catabolite activator protein (CAP) pathway. We tentatively conclude that DnaA protein is involved in the expression of the flhD gene by a mechanism independent of the cAMP-CAP pathway.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mizushima
authors.forename: R
authors.initials: R
authors.lastname: Koyanagi
authors.forename: T
authors.initials: T
authors.lastname: Katayama
authors.forename: T
authors.initials: T
authors.lastname: Miki
authors.forename: K
authors.initials: K
authors.lastname: Sekimizu
mesh_terms: D015152:Blotting, Northern; D000242:Cyclic AMP / Q000378:metabolism; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005408:Flagellin / Q000096:biosynthesis / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D009876:Operon; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D002373:Cyclic AMP Receptor Protein; D012333:RNA, Messenger; D005408:Flagellin; D000242:Cyclic AMP