title: Age-related changes in phospholipid fatty acid composition and monoaminergic neurotransmission in the hippocampus of rats fed a balanced or an n-3 polyunsaturated fatty acid-deficient diet.
abstract: The influence of aging (2, 6, 12, and 24 months) on hippocampal lipid composition and neurochemical markers (endogenous noradrenaline, serotonin levels, monoamine oxidase (MOA) activities) was studied in rats fed a control or an n-3 polyunsaturated fatty acid (PUFA)-deficient diet. The n-3 PUFA deficiency reduced the 22:6 (n-3) level, compensated by the increase in 22:5 (n-6). However, the difference in 22:6 (n-3) content between control and deficient rats was less between 2 and 12 months and then became stable. There was an overall age-induced decrease in the major phospholipid classes phosphatidylethanolamine (PE), and phosphatidylcholine (PC) whereas the minor classes, phosphatidylinositol (PI), phosphatidylserine (PS), and sphingomyelin (SM), were greatly increased, regardless of diet. The n-3 PUFA deficiency induced a reduction in the PS level, concomitant with a higher level in MAO-B activity as compared to control rats at the age of 24 months. The age-related evolution of the MAO-B activity was parallel with that of noradrenaline levels in both dietary groups. The noradrenaline and serotonin levels were modified according to age but without effect of the n-3 PUFA deficiency. Results showed that the hippocampus sustained specific age-induced modifications in lipid composition and neurotransmission factors, often with a transition period between 6 and 12 months.
authors.affiliation: INSERM U316, Laboratoire de Biophysique Médicale et Pharmaceutique, Tours, France.
authors.forename: S
authors.initials: S
authors.lastname: Delion
authors.forename: S
authors.initials: S
authors.lastname: Chalon
authors.forename: D
authors.initials: D
authors.lastname: Guilloteau
authors.forename: B
authors.initials: B
authors.lastname: Lejeune
authors.forename: J C
authors.initials: JC
authors.lastname: Besnard
authors.forename: G
authors.initials: G
authors.lastname: Durand
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D004032:Diet; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005260:Female; D006624:Hippocampus / Q000737:chemistry* / Q000502:physiology; D008995:Monoamine Oxidase / Q000378:metabolism; D009638:Norepinephrine / Q000032:analysis / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000032:analysis / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D010743:Phospholipids; D012701:Serotonin; D008995:Monoamine Oxidase; D009638:Norepinephrine