The effect of areca nut on salivary and selected oral microorganisms.
The aim of this study was to investigate the effect on the growth of salivary and selected oral microorganisms of areca nut, aqueous extracts of the nut, its major alkaloid arecoline and the components tannic acid and catechin of its tannin fraction. The antibacterial properties of the above were tested on Streptococcus mutans, Streptococcus salivarius, Candida albicans and Fusobacterium nucleatum and, as a control, Staphylococcus aureus. This was followed by investigating its effect on salivary organisms cultured from the saliva after chewing boiled areca nut. Extracts inhibited the growth of the selected organisms in a concentration dependent manner, baked and boiled nuts being significantly more potent than raw nut. Growth of C. albicans was the least affected by the nut extracts. Tannic acid was strongly antibacterial but not catechin or arecoline. No antibacterial effect could be demonstrated on salivary organisms after chewing the nut for 5 minutes but exposure of saliva to the cud for 1 hour caused a significant depression of bacterial growth. It is concluded that the hydrolysable tannins in the tannin fraction, which include tannic acid, are responsible for the antibacterial properties of the nut and that prolonged intraoral exposure to the nut can suppress bacteria in the mouth.
de Miranda
C M
CM
University of Stellenbosch, Tygerberg, South Africa.
van Wyk
C W
CW
van der Biji
P
P
Basson
N J
NJ
D000704:Analysis of Variance; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology; D001114:Areca; D001115:Arecoline / Q000008:administration & dosage / Q000494:pharmacology; D001252:Astringents / Q000008:administration & dosage / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D002392:Catechin / Q000008:administration & dosage / Q000494:pharmacology; D003296:Cooking; D004305:Dose-Response Relationship, Drug; D016967:Fusobacterium nucleatum / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D006868:Hydrolysis; D047348:Hydrolyzable Tannins / Q000008:administration & dosage / Q000494:pharmacology; D008409:Mastication; D009055:Mouth / Q000382:microbiology*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D012463:Saliva / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013291:Streptococcus / Q000187:drug effects* / Q000254:growth & development; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D001252:Astringents; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D001115:Arecoline; D002392:Catechin