The use of polymerase chain reaction (PCR) as a diagnostic tool for dengue virus.
This study describes the use of polymerase chain reaction as a diagnostic tool for detecting and typing of dengue virus. PCR was compared against virus isolation. First RT-PCR was done using dengue consensus primers after which positive samples were subjected to RT-PCR using type-specific primers. This study shows that the local strains of the dengue virus could be detected using the chosen primers. Furthermore, RT-PCR was found to be more sensitive than virus isolation in identifying the dengue positive samples.
Thayan
R
R
Division of Virology, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
Vijayamalar
B
B
Zainah
S
S
Chew
T K
TK
Morita
K
K
Sinniah
M
M
Igarashi
A
A
D016022:Case-Control Studies; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008296:Malaysia; D012367:RNA, Viral / Q000032:analysis*; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*
D012367:RNA, Viral