Effect of angiotensin-converting enzyme inhibition on Na+/H(+)-exchange and Ca(2+)-efflux in human erythrocytes.
The effect of the angiotensin-converting enzyme inhibitor, captopril, on Na+/H(+)-exchange activity and on Ca(2+)-ATPase mediated Ca(2+)-efflux was investigated in red blood cell membranes of patients with essential hypertension. The erythrocyte Na+/H(+)-exchange rate was measured as the difference in H(+)-efflux rate when measured in the presence or absence of Na+. The erythrocyte Ca(2+)-transport mediated by Ca(2+)-ATPase was assayed as the erythrocyte Ca(2+)-efflux rate. Our data show that captopril, in vitro and in vivo, inhibits the Ca(2+)-ATPase mediated Ca(2+)-efflux rate in human erythrocytes, while in vivo it stimulates the erythrocytes Na+/H(+)-exchange rate.
Lijnen
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Department of Molecular and Cardiovascular Research, University of Leuven (KULeuven), Belgium.
Arabidze
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Petrov
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D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002216:Captopril / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006973:Hypertension / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017923:Sodium-Hydrogen Exchangers / Q000187:drug effects* / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D017923:Sodium-Hydrogen Exchangers; D002216:Captopril; D002118:Calcium