A novel Arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases.
An Arthrobacter beta-galactosidase has homology with the lysosomal acid beta-galactosidases from humans and mice and with a Xanthomonas manihotis enzyme. Phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade. The gene encodes a subunit of 52 kDa, and the enzyme appears to be active as a dimer. The enzyme hydrolyzed substrates with either a beta-1,4 or a beta-1,3 linkage.
Gutshall
K
K
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
Wang
K
K
Brenchley
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014974:Xanthomonas / Q000201:enzymology / Q000235:genetics; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification
D001616:beta-Galactosidase