The A protein of the filamentous bacteriophage Cf of Xanthomonas campestris pv. citri.
Filamentous bacteriophages have very strict host specificities. Experiments were performed to investigate whether the A protein of the filamentous phage Cf, which infects Xanthomonas campestris pv. citri but not X. campestris pv. oryzae, is involved in determining Cf's host specificity. The gene encoding the A protein of Cf was cloned and expressed in X. campestris pv. citri. The genomic DNA of another filamentous bacteriophage, Xf, which infects X. campestris pv. oryzae but not X. campestris pv. citri, was then introduced by electroporation into X. campestris pv. citri that had expressed the A protein of Cf. The progeny phages thus produced were able to infect both X. campestris pv. oryzae and X. campestris pv. citri, indicating that the A protein of Cf was incorporated into the viral particles of Xf and conferred upon Xf the ability to infect the host of Cf. Inactivation of the A protein gene abolished the infectivity of Cf. The results of this study indicate that the A protein of Cf is responsible for controlling the host specificity of Cf.
Yang
M K
MK
Department of Biology, Fu Jen University, Taipei, Taiwan, Republic of China. bio1004@fujens.fju.edu.tw
Yang
Y C
YC
D001435:Bacteriophages / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D005814:Genes, Viral; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D014764:Viral Proteins / Q000096:biosynthesis / Q000378:metabolism*; D014771:Virion / Q000502:physiology; D016959:Xanthomonas campestris / Q000235:genetics / Q000821:virology*
C105794:A protein, bacteriophage Cf; D011994:Recombinant Proteins; D014764:Viral Proteins