title: Exogenous heparin induces fibronectin overexpression parallel to angiogenesis in the extracellular matrix of the chick embryo chorioallantoic membrane.
abstract: Heparin (HE) was injected into the allantoic sac of chick embryo eggs on the 5th day of incubation. After 48 h, a morphometric analysis of angiogenic response and an immunohistochemical investigation of fibronectin (FN) and type IV collagen immunoreactivity in developing vasculature were performed in order to verify whether HE-related choriollantoic membrane (CAM) angiogenic activity was associated with overexpression of FN and/or type IV collagen changes in CAM extracellular matrix. Data to be presented show a close relationship between HE treatment, angiogenic processes, and overexpression of FN, but not of type IV collagen in CAM extracellular matrix. They agree with other studies proving a facilitating role of FN in angiogenic processes.
authors.affiliation: Institute of Human Anatomy, Histology and Embryology, University of Bari, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Ribatti
authors.forename: A
authors.initials: A
authors.lastname: Vacca
authors.forename: F
authors.initials: F
authors.lastname: Costantino
authors.forename: M
authors.initials: M
authors.lastname: Minischetti
authors.forename: P
authors.initials: P
authors.lastname: Locci
authors.forename: E
authors.initials: E
authors.lastname: Becchetti
authors.forename: L
authors.initials: L
authors.lastname: Roncali
authors.forename: F
authors.initials: F
authors.lastname: Dammacco
mesh_terms: D000482:Allantois / Q000098:blood supply / Q000737:chemistry; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply* / Q000737:chemistry; D004625:Embryo, Nonmammalian / Q000098:blood supply; D005109:Extracellular Matrix / Q000737:chemistry / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D005353:Fibronectins / Q000032:analysis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D007150:Immunohistochemistry; D008833:Microcirculation / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000187:drug effects*
chemical_list: D005343:Fibrinolytic Agents; D005353:Fibronectins; D006493:Heparin