CSF of neuroleptic-naive first-episode schizophrenic patients: levels of biogenic amines, substance P, and peptides derived from chromogranin A (GE-25) and secretogranin II (secretoneurin).
Lumbar cerebrospinal fluid (CSF) was collected from controls and neuroleptic-naive patients with their first acute schizophrenic episode. The CSF was analyzed for several biogenic amines and their metabolites [dopamine,dihydroxyphenylacetic acid (DOPAC), noradrenaline, 5-hydroxytryptamine (5-HT), 5-hydroxyindolacetic acid (5-HIAA)]. For these transmitters, which are stored and secreted from synaptic vesicles, there was no significant difference between controls and schizophrenic patients. As constituents of large dense-core vesicles substance P (SP) and GE-25 (derived from chromogranin A)-and secretoneurin (derived from secretogranin 11)-immunoreactivities were determined. SP-like immunoreactivity levels did not differ between controls and patients; however, GE-25 was elevated and especially the GE-25/secretoneurin ratio was significantly (p < .001) higher in patients. Characterization of the immunoreactivities by high-performance liquid chromatography did not reveal any difference between patients (n = 3) and controls in the processing of the two proproteins chromogranin A and secretogranin II. These data indicate that proteolytic processing of the two widespread constituents of large dense-core vesicles, i.e., chromogranin A and secretogranin II, is not altered in schizophrenic patients. The increase in the chromogranin A /secretoneurin ratio in schizophrenic patients deserves further investigation in order to elucidate its possible pathogenetic significance.
Miller
C
C
Department of Biological Psychiatry, University of Innsbruck, Innsbruck, Austria.
Kirchmair
R
R
Troger
J
J
Saria
A
A
Fleischhacker
W W
WW
Fischer-Colbrie
R
R
Benzer
A
A
Winkler
H
H
D000328:Adult; D001679:Biogenic Amines / Q000134:cerebrospinal fluid*; D002851:Chromatography, High Pressure Liquid; D053379:Chromogranin A; D002864:Chromogranins / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D008297:Male; D009479:Neuropeptides / Q000134:cerebrospinal fluid*; D011569:Psychiatric Status Rating Scales; D011863:Radioimmunoassay; D012559:Schizophrenia / Q000134:cerebrospinal fluid*; D053381:Secretogranin II; D013373:Substance P / Q000134:cerebrospinal fluid*
D001679:Biogenic Amines; C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D009479:Neuropeptides; D053381:Secretogranin II; C081159:secretoneurin; D013373:Substance P