title: Use of a modified binding model for the investigation of affinity dependence on antibody concentration in immunoassay systems.
abstract: Affinity parameters obtained from standard calibration curves of radioimmunoassays (RIA) have been shown to decrease with increasing antibody concentration. A modified binding model was introduced in which not only binding capacity but also affinity was influenced by the antibody concentration. This allows a consistent overall description of RIA standards and titration curves for the characterization of binding assays.
authors.affiliation: BioTez Berlin-Buch GmbH, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Strohner
authors.forename: D
authors.initials: D
authors.lastname: Sarrach
authors.forename: J G
authors.initials: JG
authors.lastname: Reich
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood* / Q000378:metabolism*; D000915:Antibody Affinity; D001666:Binding Sites, Antibody; D004306:Dose-Response Relationship, Immunologic; D004958:Estradiol / Q000097:blood / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D018448:Models, Immunological; D011863:Radioimmunoassay / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D004958:Estradiol