Genetic alterations and oxidative metabolism in sporadic colorectal tumors from a Spanish community.
Deletions of loci on chromosomes 5q, 17p, 18q, and 22q, together with the incidence of p53 mutations and amplification of the double minute-2 gene were investigated in the sporadic colorectal tumors of 44 patients from a Spanish community. Chromosome deletions were analyzed by means of loss of heterozygosity analysis using a restriction fragment length polymorphism assay. Allelic losses were also detected by polymerase chain reaction (PCR)-single-stranded conformation polymorphism (SSCP) analysis of a polymorphic site in intron 2 of the p53 gene. The percentages of genetic deletions on the screened chromosomes were 39.3% (5q), 58.3% (17p), 40.9% (18q), and 40% (22q). Mutations in p53 exons 2-9 were examined by PCR-SSCP analysis and direct sequencing of the mutated region. Twenty of 44 tumor samples (45.45%) showed mutations at various exons except for exons 2, 3, and 9, the most frequent changes being G-->T transversion and C-->T transition. Because oxygen-free radicals play a role in the carcinogenesis process, we evaluated the oxidative status of the colorectal tumors. Antioxidant activities, lipid peroxidation, and DNA-damaged product concentrations in colon tumors and normal mucosa were compared. In tumor tissues, superoxide dismutase and catalase decreased fourfold and twofold, respectively, whereas glutathione peroxidase and reduced glutathione increased threefold. Malondialdehyde and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were twofold higher in colorectal tumors than in normal mucosa. Seven of 10 DNA tumor samples (70%) showing higher values of 8-OHdG also had genetic alterations at different chromosomal loci. In these samples, the p53 gene was deleted or mutated in 71.4% of cases. We concluded that the observed changes in the oxidative metabolism of the tumor cells and the consecutive increase in DNA damage may potentiate the genomic instability of different chromosomal regions, leading to further cell malignancy and tumor expansion.
Oliva
M R
MR
Servicio de Análisis Clínicos e Inmunología, Hospital Virgen de las Nieves, Granada, Spain.
Ripoll
F
F
Muñiz
P
P
Iradi
A
A
Trullenque
R
R
Valls
V
V
Drehmer
E
E
Sáez
G T
GT
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D002878:Chromosomes, Human, Pair 1; D002887:Chromosomes, Human, Pair 18; D002892:Chromosomes, Human, Pair 22; D002895:Chromosomes, Human, Pair 5; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry; D005260:Female; D005784:Gene Amplification; D016158:Genes, p53; D005819:Genetic Markers; D005978:Glutathione / Q000378:metabolism; D006579:Heterozygote; D006801:Humans; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins; D010084:Oxidation-Reduction; D018807:Polymorphism, Single-Stranded Conformational; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051736:Proto-Oncogene Proteins c-mdm2; D017384:Sequence Deletion; D013030:Spain
D004273:DNA, Neoplasm; D005819:Genetic Markers; D008054:Lipid Peroxides; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D000080242:8-Hydroxy-2'-Deoxyguanosine; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2; D003849:Deoxyguanosine; D005978:Glutathione