Transformation by bovine papillomavirus type 1 E6 is independent of transcriptional activation by E6.
We have generated mutants of bovine papillomavirus type 1 E6 (BE6) that are defective for transcriptional activation and have analyzed these mutants for transformation of contact-inhibited cells and association with the mammalian protein E6-AP. These BE6 mutants demonstrate that transformation by BE6 does not require transcriptional activation and that association of BE6 with E6-AP is a function separable from transcriptional activation by BE6. Association of BE6 with E6-AP appears to be necessary but not sufficient for transformation by BE6. In addition, the mutational analysis of BE6 demonstrates that transactivation, transformation, and association with E6-AP all require sequences throughout the BE6 gene, indicating that the papillomavirus E6 proteins do not have a simple domain structure.
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Institute of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA.
Allen
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Vande Pol
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D000595:Amino Acid Sequence; D000818:Animals; D052688:Bovine papillomavirus 1 / Q000472:pathogenicity*; D002417:Cattle; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004252:DNA Mutational Analysis; D003673:Defective Viruses; D015967:Gene Expression Regulation, Viral; D008025:Ligases / Q000378:metabolism; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D030361:Papillomavirus Infections / Q000821:virology*; D011485:Protein Binding; D016415:Sequence Alignment; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014412:Tumor Virus Infections / Q000821:virology*; D044767:Ubiquitin-Protein Ligases
D009856:Oncogene Proteins, Viral; C059269:protein E6, Bovine papillomavirus; D044767:Ubiquitin-Protein Ligases; D008025:Ligases