title: Processing of the Bacillus subtilis thrS leader mRNA is RNase E-dependent in Escherichia coli.
abstract: We have recently reported that processing occurs in the untranslated leader region of several members of a family of Gram-positive genes regulated by tRNA-mediated antitermination. We showed that cleavage at this site plays an important role in the induction of Bacillus subtilis thrS gene expression, following threonine starvation, by stabilising the downstream mRNA. Here we show that, when transferred on a plasmid, processing of the B. subtilis thrS leader can occur at the same site in Escherichia coli. Cleavage at this site is dependent on the E. coli endoribonuclease E, both in vivo and in vitro, suggesting that a functional homologue of RNase E is responsible for thrS processing in B. subtilis.
authors.affiliation: UPR 9073, Institut de Biologie Physico-Chimique, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Condon
authors.forename: H
authors.initials: H
authors.lastname: Putzer
authors.forename: D
authors.initials: D
authors.lastname: Luo
authors.forename: M
authors.initials: M
authors.lastname: Grunberg-Manago
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D004722:Endoribonucleases / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D013914:Threonine-tRNA Ligase / Q000235:genetics*
chemical_list: D012329:RNA, Bacterial; D012333:RNA, Messenger; D004722:Endoribonucleases; C022537:ribonuclease E; D013914:Threonine-tRNA Ligase