Ethanol potentiates the uptake of [14C]serine into phosphatidylserine by base-exchange reaction in NG 108-15 cells.
Phospholipid base-exchange enzymes catalyze the incorporation of nitrogenous bases into phosphoglycerides by a calcium-dependent mechanism. In this study, we describe the effect of ethanol on the incorporation of radioactive serine, choline and ethanolamine into their respective phospholipids in a neuroblastoma x glioma hybrid cell line (NG 108-15). Long term ethanol exposure induced a potentiation of the incorporation of [14C]serine into phosphatidylserine. Moreover, the phosphorus content of PS was found to be increased after long-term ethanol exposure. No concomitant changes in the phosphorus content of other phospholipids were observed. The results indicate that in NG 108-15 cells, the incorporation of radiolabelled serine into PS is potentiated during chronic ethanol exposure.
Rodríguez
F D
FD
Dept. of Biochemistry and Molecular Biology, University of Salamanca, Spain.
Alling
C
C
Gustavsson
L
L
D000818:Animals; D002250:Carbon Radioisotopes; D002794:Choline / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D005910:Glioma; D006822:Hybrid Cells; D007700:Kinetics; D009447:Neuroblastoma; D010718:Phosphatidylserines / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D012694:Serine / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D004983:Ethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids; D014316:Tritium; D000431:Ethanol; D012694:Serine; D019856:Ethanolamine; D002794:Choline