Ether extract of fetal calf serum protects cultured rat cortical neurons against glutamate cytotoxicity.
The effects of an ether extract of fetal calf serum (EE-FCS) on glutamate-induced cytotoxicity were examined using primary cultures of rat cortical neurons. The simultaneous addition of EE-FCS and glutamate reduced glutamate cytotoxicity in a concentration-dependent manner. EE-FCS also reduced the cytotoxicity induced by S-nitrosocysteine, a nitric oxide (NO) donor. The findings indicate that EE-FCS contains lipid-soluble, non-peptide substances with neuroprotective actions against NO-mediated glutamate neurotoxicity.
Kume
T
T
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Kohchiyama
H
H
Nishikawa
H
H
Maeda
T
T
Kaneko
S
S
Akaike
A
A
Noda
N
N
Fujita
T
T
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects*; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004987:Ethers / Q000737:chemistry; D005312:Fetal Blood / Q000378:metabolism; D018698:Glutamic Acid / Q000633:toxicity*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D026403:S-Nitrosothiols
D004987:Ethers; D026403:S-Nitrosothiols; D009569:Nitric Oxide; D018698:Glutamic Acid; C021315:S-nitrosocysteine; D003545:Cysteine