title: Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.
abstract: A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.
authors.affiliation: Laboratoire de Bactériologie, Université de Bordeaux 2, France. helico@labhel.u-bordeaux2.fr
authors.forename: L
authors.initials: L
authors.lastname: Monteiro
authors.forename: D
authors.initials: D
authors.lastname: Bonnemaison
authors.forename: A
authors.initials: A
authors.lastname: Vekris
authors.forename: K G
authors.initials: KG
authors.lastname: Petry
authors.forename: J
authors.initials: J
authors.lastname: Bonnet
authors.forename: R
authors.initials: R
authors.lastname: Vidal
authors.forename: J
authors.initials: J
authors.lastname: Cabrita
authors.forename: F
authors.initials: F
authors.lastname: Mégraud
mesh_terms: D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011134:Polysaccharides
chemical_list: D011134:Polysaccharides