Free radical production by azomethine H: effects on pancreatic and hepatic tissues.
The antimalarial properties of azomethine H represent the basis for its use as a chemotherapeutic agent. This work was carried out in order to verify the biological side effects of azomethine H and to clarify the contribution of reactive oxygen species (ROS) in this process. It was shown that azomethine H increased serum activities of amylase, alanine transaminase (ALT) and the TBARS concentrations, in rats. No changes were observed in glutathione peroxidase and catalase activities. The drug-induced tissue damage might be due to superoxide radicals (O2.-), since Cu-Zn superoxide dismutase activities were increased by azomethine H treatment. This study allows tentative conclusions to be drawn regarding which reactive oxygen metabolites play a role in azomethine H activity. We concluded that (O2.-) maybe produced as a mediator of azomethine H action.
Novelli
E L
EL
Department of Chemistry and Biochemistry, I.B., University Estadual Paulista, UNESP, São Paulo, Brazil.
Silva
A M
AM
Monteiro
J P
JP
Sacomani
L B
LB
Novellif
J L
JL
D000410:Alanine Transaminase / Q000097:blood; D000681:Amylases / Q000097:blood; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects*; D008297:Male; D009282:Naphthalenesulfonates / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D010179:Pancreas / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013882:Thiosemicarbazones / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity
D000962:Antimalarials; D005609:Free Radicals; D009282:Naphthalenesulfonates; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D013882:Thiosemicarbazones; D013481:Superoxides; C097966:azomethine H; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000410:Alanine Transaminase; D000681:Amylases