title: Differences in immunophenotyping of mucosal lymphocytes between ulcerative colitis and Crohn's disease.
abstract: OBJECTIVE
  Immunologic studies have characterized the numbers and types of inflammatory cells in diseased inflammatory bowel disease (IBD) mucosa but have yielded conflicting results regarding intestinal lymphocytes activation in IBD. We investigated the levels of lymphocytes subsets, interleukin-2 receptor, transferrin receptor, and T cell receptors in mainly isolated lamina propria lymphocytes. Including intraepithelial lymphocytes of normal colonic mucosa or IBD (ulcerative colitis and Crohn's disease) mucosa to understand the pathogenesis of IBD. We have results from this study.
  
  
  RESULTS
  1) In comparing ulcerative colitis with control, IL-2R (p < 0.05), TR (p < 0.01), and CD3/HLA-DR (< 0.05) showed a significant increase. 2) In comparing Crohn's disease with control, CD3 (P < 0.05), TCR alpha/beta (p < 0.01) and TCR gamma/delta (p < 0.05) showed a significant decrease. 3) In comparing Crohn's disease with ulcerative colitis, CD19 (p < 0.01), TR (p < 0.01), TCR alpha/beta (p < 0.01) and TCR gamma/delta (p < 0.05) showed a significant decrease.
  
  
  CONCLUSIONS
  From these results, there are increased T cell markers, IL-2R, TR, and CD3/HLA-DR in UC, but differently, decreased CD3, TCR alpha/beta and TCR gamma/delta in CD compared with control. In addition, definitive differences in lymphocytes markers, CD19, TR, TCR alpha/beta and TCR gamma/delta, which are higher in UC than in CD, may elucidate the different immunopathogenesis between UC and CD.
authors.affiliation: Department of Internal Medicine, Chon Buk National University, Chonbuk, Korea.
authors.forename: H B
authors.initials: HB
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: C Y
authors.initials: CY
authors.lastname: Yim
authors.forename: D G
authors.initials: DG
authors.lastname: Kim
authors.forename: D S
authors.initials: DS
authors.lastname: Ahn
mesh_terms: D000328:Adult; D017252:CD3 Complex / Q000032:analysis; D003093:Colitis, Ulcerative / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D003424:Crohn Disease / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D046508:Culture Techniques; D003937:Diagnosis, Differential; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000032:analysis; D011990:Receptors, Transferrin / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D017252:CD3 Complex; D006684:HLA-DR Antigens; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2; D011990:Receptors, Transferrin