title: Identification of Listeria species by a semi-nested polymerase chain reaction.
abstract: Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welshimeri.
authors.affiliation: Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Manzano
authors.forename: L
authors.initials: L
authors.lastname: Cocolin
authors.forename: P
authors.initials: P
authors.lastname: Ferroni
authors.forename: V
authors.initials: V
authors.lastname: Gasparini
authors.forename: D
authors.initials: D
authors.lastname: Narduzzi
authors.forename: C
authors.initials: C
authors.lastname: Cantoni
authors.forename: G
authors.initials: G
authors.lastname: Comi
mesh_terms: D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008087:Listeria / Q000235:genetics / Q000302:isolation & purification*; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*