Genotype determination of three dengue type 2 virus strains from Myanmar by sequencing E/NSI gene junction.
Genotype of three dengue-2 virus strains from Myanmar was determined as genotype II by sequencing 240 nucleotide long fragment across the E/NS1 gene junction by the primer extension dideoxy chain termination method, applying direct sequencing of the PCR product. These strains were isolated from a dengue shock syndrome (DSS) patient and two patients with dengue hemorrhagic fever (DHF) grade 1, in Yangon (Rangoon), Myanmar (Burma), in 1987. Sequence homology of all three strains were highest (96%) to New Guinea C strain (genotype II), lesser homology (93%) to Jamaican 1409 strain (genotype III), and the least homology (91%) to PR 159/S1 strain (genotype I). Two DHF strains revealed only 2 nucleotide and 3 nucleotide differences compared with DSS strain, all at the 3rd position of the codons which resulted in silent mutations.
Kyaw-Zin-Thant
Department of Virology, Nagasaki University, Japan. igarashi@net. nagasaki-u.ac.jp
Khin-Mar-Aye
Soe-Thein
Than-Swe
Hasebe
F
F
Morita
K
K
Igarashi
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D002052:Myanmar / Q000453:epidemiology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014759:Viral Envelope Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D004279:DNA, Viral; C100315:NS1 protein, Flavivirus; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins