Expression of the fibroblast growth factor-2 gene during chick development.
In this report, we have carried out mRNA in situ hybridization analysis to study the pattern of expression of the fibroblast growth factor-2 (FGF-2) gene during chick development. The expression pattern of FGF-2 was compared to that of the fibroblast growth factor-1 (FGF-1) gene to further assess a possible role of the FGF-2 during chick embryogenesis. The whole embryos of various stages were examined with 35S-labeled riboprobes transcribed from the coding regions of chick FGF-2 and FGF-1 cDNAs. We show that the distribution pattern of FGF-2 mRNA was highly specific. The FGF-2 transcripts were localized to limb mesenchymes, pharyngeal regions, and embryonic kidneys in day 3 to day 6 chick embryos. However, no FGF-1 mRNA was detected in these structures indicating a unique role of FGF-2 in chick development. These results support the hypothesis that FGF-2 may be a crucial signaling molecule during chick embryogenesis.
Han
J K
JK
Department of Life Sciences, Pohang University of Science and Technology, Korea.
D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D017931:DNA Primers / Q000235:genetics; D005121:Extremities / Q000196:embryology; D016220:Fibroblast Growth Factor 1 / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D010614:Pharynx / Q000196:embryology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
D017931:DNA Primers; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1