title: Reversed-phase high-performance liquid chromatographic separation of LY309887 (thienyl-5,10-dideazatetrahydrofolate) stereoisomers using beta-cyclodextrin as a mobile phase additive.
abstract: The separation of the four stereoisomers of LY309887 (thienyl-5,10-dideazatetrahydrofolate) is studied here using commercially available high-performance liquid chromatography column technology. The selectivity needed to resolve the four stereoisomers is provided by an achiral reversed-phase column used with beta-cyclodextrin as a mobile phase additive. The separation is dependent on triethylamine and beta-cyclodextrin concentration in the buffer portion of the eluent, eluent strength, and buffer pH.
authors.affiliation: Chemical Process Research and Development, Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Marks
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013763:Tetrahydrofolates / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: C105065:6R-2',5'-thienyl-5,10-dideazatetrahydrofolic acid; D004791:Enzyme Inhibitors; D013763:Tetrahydrofolates