A novel 90-kDa stress protein induced in fish cells by fish rhabdovirus infection.
A 90 kDa cellular protein in a fish cell, CHSE-214, showed increased expression by the infection of infectious hematopoietic necrosis virus (IHNV), heat shock, 2-mercaptoethanol, copper sulfate, and cadmium sulfate, and was detected in various kinds of cells such as human, rat, and mouse cells. The molecular mass of the 90 kDa protein was different from those of the hsp90 and grp94. In addition, all the anti-stress protein MAbs did not react with the 90 kDa protein. Finally, the subcellular distribution of the 90 kDa protein, determined by Western blots of subcellular fractions, was found to be mainly nuclear, both in normal and IHNV-infected CHSE-214 cells. The present results indicate that the 90 kDa protein is a kind of stress protein. However, based on its molecular mass, antigenic characteristics, and subcellular distribution, it is likely that this protein is a novel stress protein that has not been previously described in animal systems, especially in fish systems.
Cho
W J
WJ
Department of Microbiology, University of Ulsan, Kyongnam, Korea.
Cha
S J
SJ
Do
J W
JW
Choi
J Y
JY
Lee
J Y
JY
Jeong
C S
CS
Cho
K J
KJ
Choi
W S
WS
Kang
H S
HS
Kim
H D
HD
Park
J W
JW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D019187:Cadmium Compounds / Q000494:pharmacology; D002460:Cell Line; D019327:Copper Sulfate / Q000494:pharmacology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005659:Fungicides, Industrial / Q000494:pharmacology; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D051381:Rats; D018353:Rhabdoviridae Infections / Q000378:metabolism*; D012474:Salmon; D013431:Sulfates / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D019187:Cadmium Compounds; D005659:Fungicides, Industrial; D018841:HSP90 Heat-Shock Proteins; D009687:Nuclear Proteins; D013431:Sulfates; D008623:Mercaptoethanol; C037123:cadmium sulfate; D019327:Copper Sulfate