title: Granulocyte colony-stimulating factor and interleukin-6-producing lung cancer cell line, LCAM.
abstract: OBJECTIVE
  We describe a case of granulocyte colony-stimulating factor (GCSF) and interleukin-6 (IL-6)-producing lung cancer.
  
  
  METHODS
  A 53-year-old man underwent left upper lobectomy under diagnosis of lung cancer. The tumor obtained by a preoperative biopsy was analyzed.
  
  
  RESULTS
  Preoperative data showed leukocytosis with left-shift of leukocytic morphology and thrombocytosis and an elevated serum GCSF level. Histological examination revealed poorly differentiated adenocarcinoma. A cell line, named LCAM, was established from the tumor and the cytokines in the culture medium were measured by enzyme immunoassay. GCSF and IL-6 were produced in large amounts by LCAM, but granulocyte-macrophage colony-stimulating factor (GMCSF) and interleukin-3 (IL-3) were not. A proportion of LCAM expressed GCSF receptor on the cell surface, but IL-6 receptor could not be detected. LCAM proliferation was inhibited in the culture with antihuman GCSF antibody in a dose-dependent manner.
  
  
  CONCLUSIONS
  We suggest that LCAM proliferation is positively regulated by GCSF.
authors.affiliation: Division of Organ Transplantation, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Minami
authors.forename: Y
authors.initials: Y
authors.lastname: Fujii
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: R
authors.initials: R
authors.lastname: Shirakura
authors.forename: T
authors.initials: T
authors.lastname: Kido
mesh_terms: D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured
chemical_list: D015850:Interleukin-6; D016179:Granulocyte Colony-Stimulating Factor