[Preparation of control human sera with increased level of certain classes of lipoproteins using serum fractions sedimented with sulfated polyanions].
Three main classes of human serum lipoproteins (LP), very low density lipoproteins (VLDLP), low density lipoproteins (LDLP), and high density lipoproteins (HDLP) were selectively sedimented by heparin and dextran sulfate in the presence of metal ions. These data were used for the development of a method for preparation of fractions of human serum enriched with certain class of LP. Using isolated fractions serums with increased content of total cholesterol, cholesterol of HDLP and triglycerides were prepared. These fractions may be used as control samples for evaluation of corresponding parameters in native samples.
Khaĭdukova
I L
IL
Malakhov
V N
VN
D001769:Blood; D005591:Chemical Fractionation; D016264:Dextran Sulfate; D006493:Heparin; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D012016:Reference Values
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D006493:Heparin; D016264:Dextran Sulfate
Poluchenie kontrol'nykh syvorotok krovi cheloveka s povyshennym soderzhaniem lipoproteidov opredelennogo klassa s ispol'zovaniem fraktsiĭ syvorotki, osazhdennykh sul'fatirovannymi polianionami.