title: Cytotoxicity of synthetic barium hydroxyapatite.
abstract: Barium hydroxyapatite (Ba10(PO4)6(OH)2, Ba-HAp) was synthesized by a wet method using Ba(OH)2.8H2O and (NH4)2HPO4 as starting materials. The Ba-HAp obtained had a Ba/P atomic ratio of 1.76 and contained CO3 groups. The Ba-HAp was sintered at 1073 K for 12 hours. The sintered Ba-HAp had a three point bending strength of 29 MPa and Young's modulus of 27 GPa. Cytotoxicity of the sintered bodies and particles was tested using L-cells. The sintered Ba-HAp showed no cytotoxicity, and the cells were closely in contact with the surfaces of sintered Ba-HAp. Morphological observation of the cell around the Ba-HAp particles also showed no cytotoxicity. However, cell growth was inhibited by Ca adsorption on the Ba-HAp particles. These results suggested that the Ba-HAp had no cytotoxicity and can be applied as a bioactive X-ray opaque material.
authors.affiliation: 10th Research Group, National Institute for Research in Inorganic Materials, Ibaraki, Japan. kikuchi@nirim.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Kikuchi
authors.forename: A
authors.initials: A
authors.lastname: Yamazaki
authors.forename: M
authors.initials: M
authors.lastname: Akao
authors.forename: H
authors.initials: H
authors.lastname: Aoki
mesh_terms: D000327:Adsorption; D000818:Animals; D001464:Barium / Q000032:analysis; D017609:Barium Compounds / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002118:Calcium / Q000737:chemistry; D002254:Carbonates / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D003287:Contrast Media / Q000737:chemistry; D004548:Elasticity; D006882:Hydroxyapatites / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D007739:L Cells; D051379:Mice; D010710:Phosphates / Q000032:analysis / Q000737:chemistry; D018583:Pliability
chemical_list: D017609:Barium Compounds; D001672:Biocompatible Materials; D002254:Carbonates; D003287:Contrast Media; D006882:Hydroxyapatites; D010710:Phosphates; C024788:ammonium phosphate; C041297:barium hydroxyapatite; C012766:barium hydroxide; D001464:Barium; D002118:Calcium