Motility stimulant effects of prostasome inclusion in swim-up medium on cryopreserved human spermatozoa.
The chance of obtaining a fertilization and establishing a pregnancy increases with the number of motile spermatozoa that can reach and interact with the oocyte after the time of insemination. In an attempt to increase the recovery of freeze-thawed and motile spermatozoa, the swim-up medium was supplemented with prostasomes and some other effectors. Swim-up media supplemented with prostasomes were superior in comparison to the other effectors investigated in the recovery of motile spermatozoa for insemination. These results suggest that prostasome inclusion in swim-up medium might be of benefit in improving results in assisted reproductive technologies using freeze-thawed spermatozoa.
Carlsson
L
L
Department of Clinical Chemistry, University Hospital, University of Uppsala, Sweden.
Ronquist
G
G
Stridsberg
M
M
Johansson
L
L
D000626:Aminopeptidases / Q000378:metabolism; D015925:Cryopreservation; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000201:enzymology / Q000502:physiology*
D003470:Culture Media; D000626:Aminopeptidases