title: Modulatory effect of erythrocytes on the platelet reactivity to collagen in IDDM patients.
abstract: Platelets participate in the atherothrombotic complications of diabetes. Recent data demonstrate that platelet reactivity can be modulated via cell-cell interactions with erythrocytes and neutrophils. In this study, platelet reactivity was evaluated in 30 IDDM patients. We used an analytical procedure that permits an independent evaluation of platelet activation (granule release, eicosanoid formation) and platelet recruitment (pro-aggregatory activity of cell-free releasates) after platelet stimulation with collagen in the presence or absence of other blood cells. The interaction between platelets and erythrocytes (hematocrit 40%) resulted in a marked enhancement of platelet activation (5HT, betaTG, TXA2 release) and recruitment in both patients and control subjects. The erythrocyte enhancement of platelet TXA2 synthesis and recruitment was significantly higher in the patients, while no differences were detected in platelet granule release. The elevated platelet recruitment in the IDDM patients was found to be due to 1) increased susceptibility of diabetic platelets to the prothrombotic effect of erythrocytes and 2) the greater response of diabetic platelets to their own cell-free releasate. Patients with poor metabolic control (elevated HbA1c) or longer evolution time had an even greater platelet recruitment. The presence of microalbuminuria is not related to the platelet recruitment. Since platelet recruitment is an essential step in thrombus growth, its enhancement may favor thrombotic complications in IDDM.
authors.affiliation: Research Center and Department of Clinical Pathology, University Hospital La Fe, Valencia, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Vallés
authors.forename: M T
authors.initials: MT
authors.lastname: Santos
authors.forename: J
authors.initials: J
authors.lastname: Aznar
authors.forename: M
authors.initials: M
authors.lastname: Velert
authors.forename: G
authors.initials: G
authors.lastname: Barberá
authors.forename: R
authors.initials: R
authors.lastname: Carmena
mesh_terms: D000293:Adolescent; D000328:Adult; D015331:Cohort Studies; D003094:Collagen / Q000494:pharmacology*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000503:physiopathology; D004912:Erythrocytes / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology*; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D012016:Reference Values; D012701:Serotonin / Q000378:metabolism; D013931:Thromboxanes / Q000378:metabolism; D001620:beta-Thromboglobulin / Q000187:drug effects / Q000378:metabolism
chemical_list: D013931:Thromboxanes; D001620:beta-Thromboglobulin; D012701:Serotonin; D003094:Collagen