The integration host factor-DNA complex upstream of the early promoter of bacteriophage Mu is functionally symmetric.
Inversion of the ihf site in the promoter region of the early promoter of bacteriophage Mu did not influence the integration host factor (IHF)-mediated functions. IHF bound to this inverted site could counteract H-NS-mediated repression, directly activate transcription, and support lytic growth of bacteriophage Mu. This implies that the IHF heterodimer and its asymmetrical binding site form a functionally symmetrical complex.
van Ulsen
P
P
Laboratory of Molecular Genetics, Leiden University, The Netherlands.
Hillebrand
M
M
Zulianello
L
L
van de Putte
P
P
Goosen
N
N
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D016384:Consensus Sequence; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D005686:Galactokinase / Q000096:biosynthesis; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D011993:Recombinant Fusion Proteins; D005686:Galactokinase