[Novel reagent for labeling of biomolecules--activated derivative of pyrene dichromophore with excimeric fluorescence].
N-[2-(1-pyrenyl)ethyl]-1-pyrenylacetamide, bis[2-(1-pyrenyl)ethyl]amine, and N,N-bis[2-(1-pyrenyl)ethyl]succinamide were synthesized from 1-pyrenylacetic acid. These compounds contain adjacent pyrene residues and display excimer fluorescence. The latter compound, as a pentafluorophenyl ester, was used to prepare fluorescently labeled oligodeoxyribonucleotide (5)CAGGAAACAGCTATGAC. For N,N-bis[2-(1-pyrenyl)ethyl]succinamide, the excimer-to-monomer fluorescence ratio and intensity of fluorescence in water-methanol solutions changed in the presence of single-stranded and double-stranded oligonucleotides, upon attachment to an oligonucleotide, and upon hybridization of the resulting conjugate with the complementary nucleotide sequence.
Balakin
K V
KV
Korshun
V A
VA
Prokhorenko
I A
IA
Maleev
G V
GV
Kudelina
I A
IA
Gontarev
S V
SV
Berlin
Iu A
IuA
D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes
Novyĭ reagent dlia mecheniia biomolekul--aktivirovannoe proizvodnoe pirenovogo bikhromofora s éksimernoĭ fluorestsentsieĭ.