title: Detergent effects on the light-harvesting chlorophyll A/B-protein complex crystallization revealed by fluorescence depolarization.
abstract: Detergent effects on the pre-crystallization of light-harvesting chlorophyll a/b-protein complex (LHCII) were investigated through the fluorescence depolarization method. Stable LHCII crystals were formed in the media containing Triton X-100 (TX) or n-nonyl-beta-D-glucopyranoside (NG) and the crystallization efficients were dependent on their concentrations. The second virial coefficient of crystallizing system, estimated by the fluorescence depolarization analysis of LHCII, showed the most harmonious value under the condition yielding the most efficient crystallization of LHCII to suggest that some specific molecular interaction leading to the crystal growth would be induced according to the concentration of TX or NG.
authors.affiliation: Faculty of Agriculture, Institute of Biophysics, Kyushu University, Higashi-ku, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Furuichi
authors.forename: E
authors.initials: E
authors.lastname: Nishimoto
authors.forename: T
authors.initials: T
authors.lastname: Koga
authors.forename: A
authors.initials: A
authors.lastname: Takase
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
mesh_terms: D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D003460:Crystallization; D003902:Detergents / Q000494:pharmacology*; D005454:Fluorescence Polarization; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000187:drug effects*; D018724:Spinacia oleracea
chemical_list: D003902:Detergents; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D002734:Chlorophyll; C037184:chlorophyll b; D000077194:Chlorophyll A