Neurogenic substance P stimulates osteogenesis in vitro.
Previous studies have shown that there is colocalization of substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactive nerve fibers in bone, periosteum and bone marrow. Because SP may also possibly play a role in bone formation, we decided to test whether it has an osteogenic stimulating effect on developing bone in vitro. To this end, 0.4, 4 and 40 micrograms/ml of SP in BGJb medium was added daily to 3 million light density (LD) bone marrow white cells which were separated by Ficoll-Paque density gradient separation then seeded onto a previously prepared fibroblast feeder layer in Petri dishes. Seven days after adding SP, in the control without SP there were 2 bone colonies; with 0.4 micrograms of SP there were 3 colonies; with 4 micrograms there were 5 colonies; with 40 micrograms there were 7 colonies. In addition, there was an increase in the size of bone colonies in the SP-added group. The results indicated that SP had a dose-related osteogenic stimulating effect. The increase in the number and size of bone colonies by SP was probably caused by stimulating stem cell mitosis, osteoprogenitor cell differentiation or osteoblastic activity.
Shih
C
C
Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Bernard
G W
GW
D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D007552:Isotonic Solutions; D008297:Male; D009412:Nerve Fibers / Q000502:physiology; D009930:Organic Chemicals; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013373:Substance P / Q000494:pharmacology*
C022788:BGJ culture medium; D003470:Culture Media; D007552:Isotonic Solutions; D009930:Organic Chemicals; D013373:Substance P