Coincident induction of K rev-1/rap 1A, rap 1B and H-ras mRNAs in the rat spinal cord by noxious stimulation.
Two cDNA fragments, K rev-1/rap 1A and rap 1B, were amplified from total cellular RNA of the rat spinal cord by reverse transcription-polymerase chain reaction with a set of oligonucleotide primers specific for the human rap 1A cDNA. We report here using Northern blot analysis with these cDNA probes that noxious stimulation causes a marked and coincident increase in rap 1A, rap 1B and H-ras mRNAs in the rat spinal cord. This suggests that Rap 1 participates in sensory processing in spinal neurons in parallel with Ras.
Urayama
O
O
Department of Clinical and Laboratory Medicine, Akita University School of Medicine, Japan.
Murakoshi
T
T
Kubota
M
M
Hara
Y
Y
Chihara
J
J
Ikawa
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D005557:Formaldehyde; D019204:GTP-Binding Proteins / Q000096:biosynthesis*; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D010146:Pain / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*; D014158:Transcription, Genetic; D020668:rap GTP-Binding Proteins
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D005557:Formaldehyde; D019204:GTP-Binding Proteins; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); D020668:rap GTP-Binding Proteins