Activation of phospholipases C and D by the novel metabotropic glutamate receptor agonist tADA.
In hippocampal slices taken from 8-day-old rats, trans-azetidine-2,4-dicarboxylic acid (tADA), a novel glutamatergic agonist acting preferentially at class I mGluR receptors, activates phosphoinositide and phosphatidylcholine hydrolysis with widely different potencies. Inositol phosphate formation was maximally increased at 10 microM tADA (EC50: 1.2 microM), while phospholipase D activation was observed at a tADA concentration of 1 mM. This is the first report of a tADA-induced phospholipase D activity.
Klein
J
J
Department of Pharmacology, University of Mainz, Germany.
Reymann
K G
KG
Riedel
G
G
D000818:Animals; D001383:Azetidinecarboxylic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists*; D010738:Type C Phospholipases / Q000378:metabolism*
D018690:Excitatory Amino Acid Agonists; D007295:Inositol Phosphates; D018094:Receptors, Metabotropic Glutamate; C083095:azetidine-2,4-dicarboxylic acid; D001383:Azetidinecarboxylic Acid; D010738:Type C Phospholipases; D010739:Phospholipase D