title: Comparison of the nucleic acids of helical and coccoid forms of Helicobacter pylori.
abstract: The nucleic acids of the helical and coccoid forms of Helicobacter pylori were studied to determine if the coccoid forms are "viable (capable of growing) but nonculturable." Using a reference strain (NCTC 11638) and five clinical strains, the nucleic acid contents, DNA integrity, and results of PCR and reverse transcription-PCR (RT-PCR) were compared for helical H. pylori and coccoid forms induced using glycochenodeoxycholic acid or bismuth citrate. The DNA and RNA contents of the coccoid forms were respectively 6.8- and 8.1-fold lower than those of helical H. pylori after 3 days of induction and 11.5- and 14.7-fold lower after 7 days. Agarose gel electrophoresis of DNA extracted from the coccoid forms after 3 days of induction showed a smear pattern indicating DNA cleavage, whereas DNA from helical H. pylori showed a single band with a high molecular mass. After 12 days of induction, all RNA samples from 100% coccoid cultures were negative for the mRNA of urease A or the 26-kDa species-specific protein by RT-PCR. However, most RNA samples obtained after 3 or 7 days of induction were positive at low levels despite the lack of recovery from these cultures. These results suggest that the coccoid form of H. pylori has impaired genomic DNA and is in the process of cellular degeneration, thus being still alive but nonincreasable.
authors.affiliation: Institute of Medical Science and Department of Internal and Laboratory Medicine, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Narikawa
authors.forename: S
authors.initials: S
authors.lastname: Kawai
authors.forename: H
authors.initials: H
authors.lastname: Aoshima
authors.forename: O
authors.initials: O
authors.lastname: Kawamata
authors.forename: R
authors.initials: R
authors.lastname: Kawaguchi
authors.forename: K
authors.initials: K
authors.lastname: Hikiji
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: S
authors.initials: S
authors.lastname: Iino
authors.forename: Y
authors.initials: Y
authors.lastname: Mizushima
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis*; D016480:Helicobacter pylori / Q000737:chemistry* / Q000166:cytology / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D012329:RNA, Bacterial / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012680:Sensitivity and Specificity; D014510:Urease / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014510:Urease