Immunolocalization of proton-ATPase in the gills of the elasmobranch, Squalus acanthias.
Proton-ATPase was localized to mitochondria-rich cells in the interlamellar region of the gills of the elasmobranch, Squalus acanthias. Localization was accomplished using a polyclonal antibody specific for the 70 kDa subunit of the (V-type) proton-ATPase as confirmed by Western blot analysis. In addition, significant levels of N-ethymaleimide sensitive ATPase activity (0.116 +/- 0.026 mumol Pi.mg-1 protein.h-1) were also measured in crude gill membrane preparations. These data provide, for the first time, direct evidence of the localization of elements possibly involved in branchial acid-base (or ionic) regulation in elasmobranchs.
Wilson
J M
JM
Department of Zoology, University of British Columbia, Vancouver, Canada.
Randall
D J
DJ
Vogl
A W
AW
Iwama
G K
GK
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D004284:Dogfish / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D005880:Gills / Q000166:cytology / Q000201:enzymology*; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008928:Mitochondria / Q000648:ultrastructure; D017494:Proton Pumps / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000032:analysis / Q000378:metabolism*
D017494:Proton Pumps; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases