title: Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit.
abstract: Extraction of high-quality RNA from blackcurrant fruit has hitherto proved difficult, probably owing to high levels of phenolic and polysaccharide components in the berries. The procedure described here is a modification of one described for grape berries, and yields RNA suitable for in vitro translations, RNA blot analysis, and cDNA library construction.
authors.affiliation: Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Dundee, Scotland, UK.
authors.forename: M
authors.initials: M
authors.lastname: Woodhead
authors.forename: M A
authors.initials: MA
authors.lastname: Taylor
authors.forename: H V
authors.initials: HV
authors.lastname: Davies
authors.forename: R M
authors.initials: RM
authors.lastname: Brennan
authors.forename: R J
authors.initials: RJ
authors.lastname: McNicol
mesh_terms: D005638:Fruit / Q000235:genetics*; D018749:RNA, Plant / Q000302:isolation & purification*
chemical_list: D018749:RNA, Plant