Effects of a novel perfluorocarbon emulsion on neutrophil chemiluminescence in human whole blood in vitro.
The effects have been studied of a novel perfluorochemical (PFC) emulsion (18.5% perfluorodecalin, 1.5% perfluorodimorpholine propane, 2.5% lecithin) on phorbol 12-myristate 13-acetate (PMA; 100 micrograms ml-1)-induced neutrophil chemiluminescence in citrated human whole blood in vitro. A transient, dose-dependent, decrease in chemiluminescence, to a maximum of 54% after 12 min (P < 0.05), occurred when blood was pre-incubated with 10-40 microliters of the PFC emulsion, compared to saline controls. The mean (+/- s.e.m., n = 6) chemiluminescence of neutrophils incubated with 30 microliters emulsion at 12 min following PMA stimulation (9.5 +/- 1.3 mV) was significantly lower (P < 0.05) than control (24.2 +/- 2.2 mV). Incubation of blood with lecithin up to 16 mg ml-1 and Pluronic F-68 or Pluronic PE 6800 up to 65 mg ml-1 did not affect chemiluminescence.
Edwards
C M
CM
Department of Life Science, University of Nottingham, University Park, U.K.
Lowe
K C
KC
Röhlke
W
W
Geister
U
U
Reuter
P
P
Meinert
H
H
D000838:Anions / Q000378:metabolism; D001802:Blood Substitutes / Q000494:pharmacology*; D004655:Emulsions; D005466:Fluorocarbons / Q000494:pharmacology*; D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate
D000838:Anions; D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; D010713:Phosphatidylcholines; D013501:Surface-Active Agents; D013481:Superoxides; C008805:perfluorodecalin; D013755:Tetradecanoylphorbol Acetate