Characterization of neural cell lines derived from SV40 large T-induced primitive neuroectodermal tumors.
We recently reported intriguing properties of neural tumors generated by retrovirus-mediated transfer of the SV40 large T antigen into fetal rat brain transplants. Histopathologically, these neoplasms displayed characteristic features of primitive neuroectodermal tumors (PNET) and exhibited a striking potential for migration into the host brain. In the present study, four cell lines were derived from these PNETs and characterized. Two lines with an immature phenotype expressed the embryonal form of the neural cell adhesion molecule and nestin. They showed spheroid formation and delicate cell processes. The remaining cell lines had a flat, epitheloid appearance and were immunoreactive for synaptophysin, neurofilament proteins and glial fibrillary acidic protein. These cells constitute valuable tools to study the cellular origin(s) and molecular basis of PNETs, differentiation of neural progenitors and tumor cell migration in the brain.
Weggen
S
S
Department of Neuropathology, University of Bonn Medical Center, FRG.
Bayer
T A
TA
Koch
A
A
Salewski
H
H
Scheidtmann
K H
KH
Pietsch
T
T
Wiestler
O D
OD
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis / Q000276:immunology*; D001921:Brain / Q000196:embryology; D016332:Fetal Tissue Transplantation; D018014:Gene Transfer Techniques; D007150:Immunohistochemistry; D018242:Neuroectodermal Tumors, Primitive / Q000276:immunology* / Q000473:pathology*; D009474:Neurons / Q000502:physiology*; D011233:Precipitin Tests; D051381:Rats / Q000196:embryology; D011916:Rats, Inbred F344; D012190:Retroviridae / Q000235:genetics; D014407:Tumor Cells, Cultured
D000952:Antigens, Polyomavirus Transforming