title: Rapid and selective analysis of secnidazole in human plasma using high-performance liquid chromatography with ultraviolet detection.
abstract: A rapid, reproducible high-performance liquid chromatographic method for the determination of secnidazole, 5-nitroimidazole class of antiprotozoals from blood is described. Metronidazole was used as an internal standard. A simple extraction step with dichloromethane was done before chromatography on a C18 column with the wavelength fixed at 276 nm on the UV detector. Blood levels up to 500 ng/ml have been measured with good precision in the healthy volunteers after 1 g of secnidazole was administered. The present described method can readily be utilized for routine pharmacokinetic studies.
authors.affiliation: Department of Clinical Pharmacology and Therapeutics, Nizam's Institute of Medical Sciences, Panjagutta, Hyderabad, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Ravi
authors.forename: M U
authors.initials: MU
authors.lastname: Naidu
authors.forename: E C
authors.initials: EC
authors.lastname: Sekhar
authors.forename: T R
authors.initials: TR
authors.lastname: Rao
authors.forename: J C
authors.initials: JC
authors.lastname: Shobha
authors.forename: P U
authors.initials: PU
authors.lastname: Rani
authors.forename: K J
authors.initials: KJ
authors.lastname: Surya
mesh_terms: D000981:Antiprotozoal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008795:Metronidazole / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000981:Antiprotozoal Agents; D008795:Metronidazole; C016724:secnidazole