title: Electrogenic ion transport in the mouse endometrium: functional aspects of the cultured epithelium.
abstract: A primary culture of mouse endometrial epithelium grown on permeable supports was established and the electrogenic ion transport across the endometrial epithelium was studied using the short-circuit current (I(SC)) technique. Enzymatically isolated mouse endometrial cells were immunostained with epithelial cells markers, cytokeratins, indicating an epithelial origin of the culture. Mouse endometrial epithelial cells grown on Millipore filters formed polarized monolayers with junctional complexes as revealed by light and electron microscopy. The cultured monolayers exhibited an average basal I(SC) of 4.6 +/- 0.3 microA/cm2, transepithelial voltage of 2.7 +/- 0.2 mV and transepithelial resistance of 599 +/- 30 omega cm2. The basal current was reduced by 85% in Na+-free solution and 13% in Cl(-)-free solution. The basal current could also be substantially (57.7%) blocked by an apical Na+ channel blocker, amiloride (10 microM), suggesting that Na+ absorption largely contributed to the basal current. Apical addition of Cl- channel blocker, DPC (2 mM), also exhibited an inhibitory effect, 19.4%, on the basal I(SC), indicating minor involvement of Cl- secretion as compared to that of Na+ absorption. The cultured endometrial epithelium also responded to a number of secretagogues including adrenaline and forskolin with increases in the I(SC), which could involve substantial Cl- secretion. The present study has established a culture of mouse endometrial epithelium exhibiting predominantly Na+ absorption under unstimulated condition, and Cl- secretion in response to various secretagogues. This culture may be useful for studying various regulatory mechanisms of electrogenic ion transport across the endometrial epithelium.
authors.affiliation: Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: H C
authors.initials: HC
authors.lastname: Chan
authors.forename: C Q
authors.initials: CQ
authors.lastname: Liu
authors.forename: S K
authors.initials: SK
authors.lastname: Fong
authors.forename: S H
authors.initials: SH
authors.lastname: Law
authors.forename: P S
authors.initials: PS
authors.lastname: Leung
authors.forename: P Y
authors.initials: PY
authors.lastname: Leung
authors.forename: W O
authors.initials: WO
authors.lastname: Fu
authors.forename: S B
authors.initials: SB
authors.lastname: Cheng Chew
authors.forename: P Y
authors.initials: PY
authors.lastname: Wong
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002034:Bumetanide / Q000494:pharmacology; D002478:Cells, Cultured; D017097:Electric Impedance; D004717:Endometrium / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D004837:Epinephrine / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D017136:Ion Transport; D007633:Keratins / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D014667:Vasopressins / Q000494:pharmacology
chemical_list: D002034:Bumetanide; D014667:Vasopressins; D007633:Keratins; D000584:Amiloride; D000109:Acetylcholine; D004837:Epinephrine