title: Rapid reconstitution of tonoplast H(+)-translocating pyrophosphatase from cultured rice cells into liposomes.
abstract: We report the rapid and functional reconstitution of H(+)-pyrophosphatase (H(+)-PPase) from the tonoplast of cultured rice (Oryza sativa L.) cells to proteoliposomes. The CHAPS-solubilized H(+)-PPase was incorporated into liposomes by gel-filtration. Both the activities of PPi-hydrolysis and H(+)-pumping were influenced by the lipid-protein ratio and cholesterol.
authors.affiliation: Molecular Function Laboratory, National Food Research Institute, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Kasamo
mesh_terms: D015153:Blotting, Western; D002784:Cholesterol / Q000494:pharmacology; D002850:Chromatography, Gel; D043564:Inorganic Pyrophosphatase; D007700:Kinetics; D008081:Liposomes; D012275:Oryza / Q000201:enzymology*; D011510:Proteolipids / Q000378:metabolism*; D011755:Pyrophosphatases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D008081:Liposomes; D011510:Proteolipids; C020485:proteoliposomes; D002784:Cholesterol; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase