Isolation of isoflavones from soy-based fermentations of the erythromycin-producing bacterium Saccharopolyspora erythraea.
A search for an abundant and economical source of isoflavones, particularly genistein, led to the discovery that the erythromycin-producing organism Saccharopolyspora erythraea also produces this promising new cancer-prevention agent. Erythromycin fermentation is a large-scale, soybean-based process used world-wide for the commercial production of this medically important antibiotic. Results from this study indicate that genistin (the glucoside form of genistein), which is added to the fermentation in the soybean media, was converted to genistein through the action of a beta-glucosidase produced by the organism. Genistein was co-extracted with erythromycin from the fermentation broth, then separated from erythromycin during the second step of the purification process for the production of erythromycin.
Hessler
P E
PE
Fermalogic, Inc., Chicago, IL 60612, USA.
Larsen
P E
PE
Constantinou
A I
AI
Schram
K H
KH
Weber
J M
JM
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003470:Culture Media / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004917:Erythromycin / Q000096:biosynthesis; D005285:Fermentation; D019833:Genistein; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D013058:Mass Spectrometry; D015394:Molecular Structure; D016930:Saccharopolyspora / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D003470:Culture Media; D007529:Isoflavones; C040641:genistin; D004917:Erythromycin; D019833:Genistein; D001617:beta-Glucosidase; D004250:DNA Topoisomerases, Type II