title: Comparative physiology of salt tolerance in Candida tropicalis and Saccharomyces cerevisiae.
abstract: The salt tolerance of the respiratory yeast Candida tropicalis and the fermentative yeast Saccharomyces cerevisiae have been compared in glucose media. C. tropicalis showed a better adaptation to Na+ and Li+ and maintained higher intracellular K+:Na+ and K+:Li+ ratios than S. cerevisiae. However, C. tropicalis showed a poorer adaptation to osmotic stress (produced by KCl and sorbitol) and exhibited reduced glycerol production as compared to S. cerevisiae. In media with the non-repressing sugar galactose as carbon source, S. cerevisiae exhibited reduced glycerol production and increased sensitivity to osmotic stress. Under these conditions, S. cerevisiae, but not C. tropicalis, utilized trehalose as a more important osmolyte than glycerol. These results suggest that the relative tolerance of yeast to the osmotic and cation toxicities of NaCl, and the underlying relative capabilities for osmolyte synthesis and cation transport, are modulated by the general catabolite control exerted by glucose.
authors.affiliation: Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: García
authors.affiliation: Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Gabino
authors.initials: G
authors.lastname: Ríos
authors.affiliation: Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Rashid
authors.initials: R
authors.lastname: Ali
authors.affiliation: Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Bellés
authors.affiliation: Institute de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia-CSIC, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Serrano
mesh_terms: D000222:Adaptation, Physiological; D002175:Candida / Q000502:physiology*; D005990:Glycerol / Q000378:metabolism; D006706:Homeostasis; D018021:Lithium Chloride / Q000378:metabolism / Q000494:pharmacology; D009997:Osmotic Pressure; D011189:Potassium Chloride / Q000378:metabolism / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D012492:Salts / Q000378:metabolism* / Q000494:pharmacology; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D013012:Sorbitol / Q000378:metabolism / Q000494:pharmacology; D013045:Species Specificity; D014199:Trehalose / Q000378:metabolism
chemical_list: D012492:Salts; D012965:Sodium Chloride; D013012:Sorbitol; D011189:Potassium Chloride; D014199:Trehalose; D018021:Lithium Chloride; D005990:Glycerol