title: Identification of Angiostrongylus cantonensis in infected rats with a coagglutination assay.
abstract: The presence of Angiostrongylus cantonensis excretory secretory (ES) products was detected in the cerebrospinal fluid (CSF) of infected rats using a coagglutination assay. There was clear agglutination in 100% of CSF samples tested of the rats infected with third-stage larvae of A. cantonensis and neither of the 20 non-infected rats produced agglutination. The assay detected 250 ng/ml or more of ES products. This assay is simple, easy to perform with minimum training and requires no equipment.
authors.affiliation: Department of Parasitology, Institute of Tropical Medicine Pedro Kouri, Havana, Cuba.
authors.forename: L
authors.initials: L
authors.lastname: Rojas Rivero
authors.forename: G L
authors.initials: GL
authors.lastname: Fonte
authors.forename: V C
authors.initials: VC
authors.lastname: Finlay
mesh_terms: D000372:Agglutination Tests / Q000379:methods*; D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000134:cerebrospinal fluid*; D001681:Biological Assay; D004195:Disease Models, Animal; D011817:Rabbits; D051381:Rats / Q000469:parasitology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017206:Strongylida Infections / Q000134:cerebrospinal fluid* / Q000276:immunology
chemical_list: D000947:Antigens, Helminth