Involvement of a common 10-amino-acid segment in the cytoplasmic region of CD40 but different MAP kinases in different CD40-mediated responses.
CD40-mediated signals can induce cell aggregation, proliferation and rescue from apoptosis in WEHI231. To define which segment of cytoplasmic domain of CD40 and how signals are involved in those events, we generated mutant CD40 transfectants. We demonstrated the same 10 amino acid segment that could bind to tumor necrosis factor receptor associated factor-2 and -3 mediated all those responses. However, activation pattern of mitogen activated protein kinases was different. Immunoglobulin M-mediated apoptosis was inhibited by CD40-mediated signal that activated c-Jun aminoterminal kinase synergistically. While, CD40 stimulus through the 10 amino acid segment alone that induced cell aggregation and proliferation resulted in activation of extracellular signal-regulated protein kinase 2.
Hara
T
T
Section of Immunopathogenesis, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo, Japan.
Murakami
M
M
Maeda
H
H
Hibi
M
M
Uede
T
T
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000276:immunology; D019013:CD40 Antigens / Q000737:chemistry / Q000276:immunology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000737:chemistry* / Q000378:metabolism; D003593:Cytoplasm / Q000276:immunology*; D004789:Enzyme Activation; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011506:Proteins / Q000737:chemistry; D048008:TNF Receptor-Associated Factor 3
D019013:CD40 Antigens; D007075:Immunoglobulin M; D011506:Proteins; D048008:TNF Receptor-Associated Factor 3; D017871:Calcium-Calmodulin-Dependent Protein Kinases