Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter junii strain 65.
A polysaccharide containing rhamnose (Rha) and Gal was isolated by acetic acid hydrolysis, followed by gel-permeation chromatography, from the water-soluble lipopolysaccharide (phenol/water extracted) from Acinetobacter junii strain 65. The polysaccharide was characterised by means of monosaccharide analyses, Smith degradation, and NMR studies, and was shown to have a linear pentasaccharide repeating unit, as depicted below. This structure was specifically recognised in western blots and enzyme immunoassays by polyclonal rabbit antisera. [structure in text]
Haseley
S R
SR
Division of Medical and Biochemical Microbiology, Research Centre Borstel, Centre for Medicine and Biosciences, Germany.
Pantophlet
R
R
Brade
L
L
Holst
O
O
Brade
H
H
D000150:Acinetobacter / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D000818:Animals; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007124:Immunoenzyme Techniques; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011817:Rabbits; D012210:Rhamnose / Q000032:analysis
D019081:O Antigens; C106366:O-antigen, Actinetobacter junii strain 65; D012210:Rhamnose; D005690:Galactose