Vitellogenin titres in normal and accelerated maturation of gregarious-phase Schistocerca gregaria.
Isolation of vitellogenin of the Schistocerca gregaria (Forskal) in its gregarious phase was achieved by a combination of gel permeation and anion exchange chromatography. Staining for carbohydrate and lipid moieties showed that the vitellogenin is a glycolipoprotein. The vitellogenin of S. gregaria has a native molecular weight of about 700 kDa. On SDS-PAGE, the protein showed nine apoproteins of about 124, 120, 105, 60, 59, 58, 57, 53 and 34 kD. Determination of the levels of vitellogenin by ELISA in the haemolymph of maturing females showed that those exposed to mature males from 1 to 2 days after ecdysis had increased levels of vitellogenin from day 10 (81.1 +/- 4.5). In contrast, females exposed to immature males or kept alone showed an increase (107.3 +/- 0.9 and 70.2 +/- 2.7) not until day 16 or later, respectively. These results are consistent with the accelerating effect of pheromonal emissions from mature males on the maturation of female S. gregaria.
Mahamat
H
H
International Centre of Insect Physiology and Ecology, Nairobi, Kenya. ICIPE@cgnet.com
Hassanali
A
A
Ferenz
H J
HJ
D000818:Animals; D004777:Environment; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006110:Grasshoppers / Q000254:growth & development* / Q000502:physiology; D006458:Hemolymph / Q000737:chemistry; D008297:Male; D010052:Ovariectomy; D013997:Time Factors; D014819:Vitellogenins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*
D014819:Vitellogenins