Gel microdrop technology for rapid isolation of rare and high producer cells.
Secreted proteins are a therapeutic cornerstone of the biotechnology industry, and numerous recombinant products, including human growth hormone, human erythropoietin and granulocyte-colony stimulating factor, are now widely prescribed. In addition to bioprocessing applications, GMD technology should benefit the emerging fields of cellular and gene therapy. The ability to assess rapidly and precisely the productivity of cells for ex vivo cell screening and expansion is a unique approach with numerous research and therapeutic uses.
Weaver
J C
JC
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge 02139, USA.
McGrath
P
P
Adams
S
S
D000911:Antibodies, Monoclonal / Q000032:analysis; D001710:Biotin; D002469:Cell Separation / Q000379:methods*; D002999:Clone Cells; D005434:Flow Cytometry / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect; D005782:Gels; D006801:Humans; D007202:Indicators and Reagents; D011994:Recombinant Proteins / Q000032:analysis; D012685:Sepharose
D000911:Antibodies, Monoclonal; D005782:Gels; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D001710:Biotin; D012685:Sepharose