Inhibition of the rat adrenal ornithine decarboxylase activity by immobilization stress and/or dexamethasone.
The effects of immobilization stress and/or dexamethasone (DEX) on the adrenal ornithine decarboxylase (ODC) activities of sham-operated and adrenal-medulloectomized (enucleated) male Sprague-Dawley rats were investigated. On day 11 after surgery, rats were injected with saline or DEX (1 mg/kg), 3 h before the time of sacrifice (0600 h or 1800 h). Four groups, from sham-operated and enucleated rats (ENU) treated with saline or DEX were subjected to immobilization stress for 1 h prior to sacrifice. Groups of rats from stress-sham-DEX, non stress-sham-DEX, stress-sham, non stress-sham, stress-ENU-DEX, non stress-ENU-DEX, stress-ENU, and non stress-ENU were sacrificed at 0600 h or 1800 h on day 11 after surgery. Adrenal glands were excised and later analyzed for ODC activities. Results indicated that DEX and/or immobilization stress inhibited ODC activities (p<0.05) in normal and regenerating adrenal glands at 1800 h and ODC activity varies diurnally, the activity being greater at 1800 h than at 0600 hours (p<0.001).
Soliman
K F
KF
Florida A&M University College of Pharmacy and Pharmaceutical Sciences, Tallahassee 32307, USA.
Reams
R R
RR
Udoye
M O
MO
Nonavinakere
V K
VK
D000311:Adrenal Glands / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D007103:Immobilization; D008297:Male; D009955:Ornithine Decarboxylase / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000378:metabolism*
D003907:Dexamethasone; D009955:Ornithine Decarboxylase