PCR identification of class I major histocompatibility complex genes transcribed in mouse blastocyst and placenta.
We used an RT-PCR based strategy to amplify, clone and sequence MHC class I genes transcribed in the blastocyst and placenta of BALB/c mice. The PCR primers used were capable of amplifying many novel class I sequences from genomic DNA. By comparing the resulting sequence data with known class I sequences, we identified a number of different class I genes transcribed in these tissues. These include H2-K, -D, -L and a novel sequence in blastocysts, and H2-K, -D, -L, -D2, -T9, -T13, -T17, -T18, -M2 and three additional novel sequences in placenta. We postulate that some members of this spectrum of blastocyst and placentally-expressed MHC class Ib genes may act together at the maternal-fetal interface in ways that are important for a successful pregnancy.
Landel
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Department of Clinical Science, Alfred I. duPont Institute, Wilmington, DE 19899, USA. landel@helios.medsci.udel.edu
Stabley
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Bundesen
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000276:immunology* / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000276:immunology; D005805:Genes, MHC Class I / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010920:Placenta / Q000276:immunology* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic / Q000276:immunology*
D012333:RNA, Messenger