title: Comparison of field and vaccine strains of Australian fowlpox viruses.
abstract: The mild fowlpox vaccine, FPV M, widely used in Australia is composed of two predominant genotypes based upon differences identifiable in restriction enzyme analyses of plaque purified derivatives of this vaccine. The differences, where identifiable, were in the end fragments of the genomes. Five field isolates of FPV from chickens in New South Wales showed restriction enzyme profiles closely related to the more virulent (standard) vaccine strain, FPV S. The FPV S strain differs from FPV M in both terminal genome fragments and in the presence of a PstI fragment of approximately 10kb (this fragment was also present in PstI digests of all of the field isolates). Plaque purified derivatives of FPV M showed similar lesion development upon inoculation into the wing web of chickens. The field isolates showed significantly higher virulence in day-old and three-week-old chickens in comparison with FPV M. One field isolate was similar to the FPV S vaccine. Two isolates had slowly developing wing web lesions, caused significant secondary lesions in three-week-old chickens and generalised poxvirus infection when inoculated into day-old chickens. For two isolates, the primary wing web lesion took even longer to develop and resolve although these isolates did not cause generalised poxvirus infection. It was possible to identify four virulence/pathogenicity types amongst these vaccine and field isolates of FPV. These strains may allow the characterisation of FPV encoded virulence factors. The field strains with higher virulence may be suitable as parent strains for the construction of FPV recombinants with enhanced immune responses to co-expressed vaccine antigens when compared with current FPV M strain based recombinants.
authors.affiliation: CSIRO, Division of Animal Health, Australian Animal Health Laboratory, Geelong, Victoria, Australia.
authors.forename: D B
authors.initials: DB
authors.lastname: Boyle
authors.forename: A D
authors.initials: AD
authors.lastname: Pye
authors.forename: B E
authors.initials: BE
authors.lastname: Coupar
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D018150:Avipoxvirus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D002478:Cells, Cultured; D002642:Chick Embryo; D004279:DNA, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D015183:Restriction Mapping; D014765:Viral Vaccines; D014774:Virulence
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D014765:Viral Vaccines