Characterization of a new isolate of Chlamydia trachomatis which lacks the common plasmid and has properties of biovar trachoma.
A Chlamydia trachomatis urethral isolate, alpha/95, yielding pgp3-negative but otherwise normal inclusions by immunofluorescence also gave negative results when pCT-homologous DNA was searched by PCR and Southern blotting. omp-1 sequence analysis identified alpha/95 as a new genotype B variant. These findings confirm that pCT is not required for chlamydial growth in vitro.
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Sezione di Microbiologia, DMCSS, Policlinico S. Orsola, Università di Bologna, Italy.
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Cevenini
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D000595:Amino Acid Sequence; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014141:Trachoma / Q000382:microbiology*
D004269:DNA, Bacterial