title: Interleukin-12- and interleukin-2-stimulated release of interferon-gamma by uterine CD56++ large granular lymphocytes is amplified by decidual macrophages.
abstract: Numerous studies have suggested that interferon-gamma (IFN-gamma) exhibits an inhibitory effect on conceptus development during pregnancy, and recent investigations have shown that decidual CD56++, CD16- large granular lymphocytes (LGL) contain mRNA for IFN-gamma. We have investigated the influence of exogenous interleukin-12 (IL-12) and interleukin-2 (IL-2) on IFN-gamma secretion by cultivated LGL and macrophages isolated from first trimester human decidua. The effect of decidual macrophages on IFN-gamma secretion by LGL was also assessed using co-incubation experiments. Neither IL-12 nor IL-2 stimulated the secretion of IFN-gamma by decidual macrophages. IL-12 alone, but not IL-2 alone, stimulated the release of IFN-gamma by LGL. However, IL-2 acted synergistically with IL-12 to enhance the release of IFN-gamma by LGL. Unstimulated and IL-12- and IL-2-stimulated LGL incubated with macrophages exhibited a marked increase in secretion of IFN-gamma compared to those in monoculture. This effect was also seen when the LGL and macrophages were separated by a semi-permeable membrane. The results suggest that interactions between decidual LGL and macrophages, possibly mediated by soluble factors, could play a role in regulating IFN-gamma secretion at the materno-fetal interface and thus contribute to the control of invasion by the trophoblast.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Wurzburg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Marzusch
authors.forename: F
authors.initials: F
authors.lastname: Buchholz
authors.forename: P
authors.initials: P
authors.lastname: Ruck
authors.forename: R
authors.initials: R
authors.lastname: Handgretinger
authors.forename: A
authors.initials: A
authors.lastname: Geiselhart
authors.forename: L
authors.initials: L
authors.lastname: Engelmann
authors.forename: J
authors.initials: J
authors.lastname: Dietl
mesh_terms: D019002:CD56 Antigen / Q000276:immunology; D003656:Decidua / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism*; D018664:Interleukin-12 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology*; D008264:Macrophages / Q000276:immunology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*
chemical_list: D019002:CD56 Antigen; D007376:Interleukin-2; D018950:Lipopolysaccharide Receptors; D018664:Interleukin-12; D007371:Interferon-gamma