Structurally similar Drosophila alpha-tubulins are functionally distinct in vivo.
We used transgenic analysis in Drosophila to compare the ability of two structurally similar alpha-tubulin isoforms to support microtubule assembly in vivo. Our data revealed that even closely related alpha-tubulin isoforms have different functional capacities. Thus, in multicellular organisms, even small changes in tubulin structure may have important consequences for regulation of the microtubule cytoskeleton. In spermatogenesis, all microtubule functions in the postmitotic male germ cells are carried out by a single tubulin heterodimer composed of the major Drosophila alpha-84B tubulin isoform and the testis-specific beta 2-tubulin isoform. We tested the ability of the developmentally regulated alpha 85E-tubulin isoform to replace alpha 84B in spermatogenesis. Even though it is 98% similar in sequence, alpha 85E is not functionally equivalent to alpha 84B. alpha 85E can support some functional microtubules in the male germ cells, but alpha 85E causes dominant male sterility if it makes up more than one-half of the total alpha-tubulin pool in the spermatids. alpha 85E does not disrupt meiotic spindle or cytoplasmic microtubules but causes defects in morphogenesis of the two classes of singlet microtubules in the sperm tail axoneme, the central pair and the accessory microtubules. Axonemal defects caused by alpha 85E are precisely reciprocal to dominant defects in doublet microtubules we observed in a previous study of ectopic germ-line expression of the developmentally regulated beta 3-tubulin isoform. These data demonstrate that the doublet and singlet axoneme microtubules have different requirements for alpha- and beta-tubulin structure. In their normal sites of expression, alpha 85E and beta 3 are coexpressed during differentiation of several somatic cell types, suggesting that alpha 85E and beta 3 might form a specialized heterodimer. Our tests of different alpha-beta pairs in spermatogenesis did not support this model. We conclude that if alpha 85E and beta 3 have specialized properties required for their normal functions, they act independently to modulate the properties of microtubules into which they are incorporated.
Hutchens
J A
JA
Department of Biology, Indiana University, Bloomington 47405, USA.
Hoyle
H D
HD
Turner
F R
FR
Raff
E C
EC
D000818:Animals; D002478:Cells, Cultured; D019281:Dimerization; D004330:Drosophila; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005799:Genes, Dominant; D005854:Germ Cells / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D007536:Isomerism; D008297:Male; D008870:Microtubules / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013329:Structure-Activity Relationship; D014404:Tubulin / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D014404:Tubulin