title: A subgenomic mRNA transcript of the coronavirus mouse hepatitis virus strain A59 defective interfering (DI) RNA is packaged when it contains the DI packaging signal.
abstract: In infected cells, only the genomic RNA of the coronavirus mouse hepatitis virus strain A59 (MHV-A59) is packaged into the virions. In this study, we show that a subgenomic (sg) defective interfering (DI) RNA can be packaged into virions when it contains the DI RNA packaging signal (DI RNA-Ps). However, the sg DI RNA is packaged less efficiently than the DI genomic RNA. Thus, while specificity of packaging of RNAs into MHV-A59 virions is determined by the DI RNA-Ps, efficiency of packaging is determined by additional factors.
authors.affiliation: Department of Virology, Leiden University, The Netherlands.
authors.forename: E C
authors.initials: EC
authors.lastname: Bos
authors.forename: J C
authors.initials: JC
authors.lastname: Dobbe
authors.forename: W
authors.initials: W
authors.lastname: Luytjes
authors.forename: W J
authors.initials: WJ
authors.lastname: Spaan
mesh_terms: D000818:Animals; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology*; D007739:L Cells; D051379:Mice; D006517:Murine hepatitis virus / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger; D012367:RNA, Viral; D019065:Virus Assembly
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral