title: Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells.
abstract: The electrophysiological effects of both trypsinactivated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patchclamp technique. Rates of reduction in whole-cell membrane resistance were correlated with increasing native toxin concentration. The 42- or 51-kDa cloned toxin alone at 50 micrograms/ml reduced the resistance. Electrophysiological effects occurred before any changes were visible by phase-contrast microscopy.
authors.affiliation: Department of Biology, Ankara University, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Cokmus
authors.forename: E W
authors.initials: EW
authors.lastname: Davidson
authors.forename: K
authors.initials: K
authors.lastname: Cooper
mesh_terms: D000818:Animals; D001407:Bacillus; D001427:Bacterial Toxins / Q000633:toxicity*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003465:Culex / Q000187:drug effects*; D004594:Electrophysiology; D007306:Insecticides / Q000633:toxicity*; D018408:Patch-Clamp Techniques
chemical_list: D001427:Bacterial Toxins; D007306:Insecticides; C046134:binB protein, Bacillus sphaericus