Activation of p42 mitogen-activated protein kinase by glutamate in cultured radial glia.
The effect of L-glutamate (Glu) and its structural analogs N-methyl-D-aspartate (NMDA), kainate (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), on the activation of p42 mitogen activated protein kinase (MAPK) was examined in cultured chick radial glia cells, namely retinal Muller cells and cerebellar Bergmann cells. Glu, NMDA, AMPA and KA evoked a dose and time dependent increase in MAPK activity. AMPA and KA responses were blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) whereas NMDA responses were sensitive to 3-[(RS)-2-carboxypiperazin-4-yl)]-propyl-1-phosphonate (CPP) indicating that the increase in MAPK activity is mediated by AMPA/low affinity KA and NMDA subtypes of Glu receptors. The present findings open the possibility of a MAPK cascade involvement in the regulation of Glu-induced gene expression in radial glia.
López-Colomé
A M
AM
Departamento de Neurociencias, Instituto de Fisiología Celular-UNAM.
Ortega
A
A
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D004353:Drug Evaluation, Preclinical; D004789:Enzyme Activation; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology*; D019950:Mitogen-Activated Protein Kinase 1; D009457:Neuroglia / Q000187:drug effects* / Q000201:enzymology; D011505:Protein-Tyrosine Kinases / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
D018690:Excitatory Amino Acid Agonists; D011994:Recombinant Proteins; D018698:Glutamic Acid; D011505:Protein-Tyrosine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1