Effect of gamma-interferon on binding of gliadin and other food peptides to the human intestinal cell line HT-29.
gamma-Interferon is one of the main cytokines released during activation of intestinal lymphocytes in coeliac patients. The question has never been addressed whether gamma-interferon influences binding of gliadin and other food peptides to human enterocytes. Therefore, the human intestinal epithelial cell line HT-29 was cultured with gliadin, casein, beta-lactoglobulin and ovalbumin, with or without gamma-interferon, and peptide binding to cells was determined by flow cytometry and fluorescence microscopy. gamma-Interferon stimulated gliadin binding by a factor of 4. Binding was saturable with half maximal binding at 0.15 mg/ml. For maximal binding, an incubation of at least 24 h was necessary. gamma-Interferon increased binding of beta-lactoglobulin and casein, too, but inhibited that of ovalbumin. Binding of gliadin was inhibited by the other peptides. Under the conditions of ongoing mucosal inflammatory reactions and release of gamma-interferon, enhanced binding may trigger intestinal lymphocytes, increase secretion of cytokines and thus induce a vicious circle.
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Institute of Clinical Chemistry and Pathobiochemistry, Medical Faculty of the University, Leipzig, Germany.
Lentz
S
S
Rothschild
M
M
Lehmann
I
I
Osman
A A
AA
Mothes
T
T
D002364:Caseins / Q000187:drug effects / Q000378:metabolism; D018929:Cell Culture Techniques; D005434:Flow Cytometry; D005502:Food / Q000009:adverse effects; D005903:Gliadin / Q000187:drug effects* / Q000378:metabolism*; D019073:HT29 Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007782:Lactoglobulins / Q000187:drug effects / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010047:Ovalbumin / Q000187:drug effects / Q000378:metabolism; D010455:Peptides / Q000187:drug effects* / Q000378:metabolism; D011485:Protein Binding
D002364:Caseins; D007782:Lactoglobulins; D010455:Peptides; D007371:Interferon-gamma; D010047:Ovalbumin; D005903:Gliadin