title: [Establishment of a new human ovarian serous cystadenocarcinoma cell line (IM), and influence on cell proliferations by cisplatin with or without hyperthermia].
abstract: A new cell line, designated IM has been established from operation material derived from a woman with ovarian serous cystadenocarcinoma. The population doubling time of the 35th passage IM cell was 28.8 hours. And it was successively subcultured 165 times in over 7 years, moreover still kept CA125 production. The nuclear DNA and cell surface CA125 antigen were double stained by propidium iodide and anti CA125 monoclonal antibody-FITC. Then the two color cytogram obtained by flow cytometry was drawn up. For the most part of CA125 positive cell retained G0 + G1 of cell cycle, the lesser part was in G2 + M phase. The S phase rate of IM cell incubated with cisplatin at 37 degrees C or 41 degrees C for 1 hour that estimated by BrdU-propidium iodide double stain method of flow cytometry, it was suggested that the inhibition of DNA synthesis by cisplatin was increased with 41 degrees C low hyperthermia.
authors.affiliation: Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Yokohama City Seibu Hospital, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iida
authors.forename: N
authors.initials: N
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Kuramoto
authors.forename: T
authors.initials: T
authors.lastname: Shimoda
authors.forename: M
authors.initials: M
authors.lastname: Hamano
authors.forename: H
authors.initials: H
authors.lastname: Hata
authors.forename: K
authors.initials: K
authors.lastname: Yonamine
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D018394:CA-125 Antigen / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology*; D018284:Cystadenocarcinoma, Serous / Q000473:pathology*; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D018394:CA-125 Antigen; D002945:Cisplatin