Characterization of 30-kDa fragments derived from beta-conglycinin degradation process during germination and seedling growth of soybean.
The degradation process of beta-conglycinin, a vicilin-type glycosylated storage protein of soybean seeds, during germination and seedling growth was examined by concanavalin A blotting combined with polyacrylamide gel electrophoresis. We detected and analyzed the structures of key intermediary fragments of 30 kDa derived from beta-conglycinin degradation, they were proved to be single-domain type subunits of beta-conglycinin. We show here a degradation process of beta-conglycinin: beta-conglycinin is subjected to limited proteolysis at exposed regions on the molecular surface, like domain junctions, generating 30-kDa single-domain fragments before non-specific proteolysis.
Kawai
M
M
Food Research & Development Laboratories, Ajinomoto Co., Inc., Japan.
Suzuki
S
S
Asano
M
M
Miwa
T
T
Shibai
H
H
D000595:Amino Acid Sequence; D052179:Antigens, Plant; D003208:Concanavalin A / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D018525:Germination / Q000502:physiology*; D005916:Globulins / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism; D037121:Plant Lectins; D055314:Seed Storage Proteins; D012639:Seeds / Q000254:growth & development / Q000378:metabolism*; D030262:Soybean Proteins; D013025:Glycine max / Q000254:growth & development / Q000378:metabolism*
D052179:Antigens, Plant; D005916:Globulins; D010446:Peptide Fragments; D010936:Plant Extracts; D037121:Plant Lectins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D003208:Concanavalin A; C011474:glycinin