Brucella abortus siderophore 2,3-dihydroxybenzoic acid protects brucellae from killing by macrophages.
Addition of 2,3-dihydroxybenzoic acid, a siderophore produced by Brucella abortus, to macrophage cultures prevented intracellular killing of brucellae during the first 12 h after infection and increased the number of intracellular brucellae recovered at 48 h after infection. The protective effect could be demonstrated with inflammatory macrophages, interferon-gamma-activated macrophages and with macrophages supplemented with iron, shown elsewhere to facilitate killing of B abortus.
Leonard
B A
BA
Department of Microbiology, Ohio State University, Columbus 43210, USA.
Lopez-Goni
I
I
Baldwin
C L
CL
D000818:Animals; D001667:Binding, Competitive; D002003:Brucella abortus / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D003676:Deferoxamine / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D062385:Hydroxybenzoates / Q000378:metabolism / Q000494:pharmacology*; D007501:Iron / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D016897:Respiratory Burst; D017262:Siderophores / Q000378:metabolism / Q000494:pharmacology*
D062385:Hydroxybenzoates; D017262:Siderophores; C009135:2,3-dihydroxybenzoic acid; D007501:Iron; D003676:Deferoxamine