Induction of MHC class I expression by the MHC class II transactivator CIITA.
Major histocompatibility complex (MHC) class I-deficient cell lines were used to demonstrate that the MHC class II transactivator (CIITA) can induce surface expression of MHC class I molecules. CIITA induces the promoter of MHC class I heavy chain genes. The site alpha DNA element is the target for CIITA-induced transactivation of class I. In addition, interferon-gamma (IFNgamma)-induced MHC class I expression also requires an intact site alpha. The G3A cell line, which is defective in CIITA induction, does not induce MHC class I antigen and promoter in response to IFNgamma. Trans-dominant-negative forms of CIITA reduce class I MHC promoter function and surface antigen expression. Collectively, these data argue that CIITA has a role in class I MHC gene induction.
Martin
B K
BK
Lineberger Comprehensive Cancer Center, Department of Microbiology-Immunology, University of North Carolina at Chapel Hill, 27599-7295, USA.
Chin
K C
KC
Olsen
J C
JC
Skinner
C A
CA
Dey
A
A
Ozato
K
K
Ting
J P
JP
D001943:Breast Neoplasms; D002277:Carcinoma; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic; D005802:Genes, MHC Class II / Q000502:physiology*; D017930:Genes, Reporter; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D009154:Mutation; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D015395:Histocompatibility Antigens Class I; C083728:MHC class II transactivator protein; D009687:Nuclear Proteins; D015534:Trans-Activators; D015500:Chloramphenicol O-Acetyltransferase