Rhabdovirus-induced apoptosis in a fish cell line is inhibited by a human endogenous acid cysteine proteinase inhibitor.
To determine the mechanisms of cell death in rhabdovirus-infected cells, we studied the infection of the epithelial papilloma of carp cell line with spring viremia of carp virus. Studies using electron microscopy, confocal microscopy, and agarose gel electrophoresis revealed changes in cell morphology and DNA fragmentation indicative of apoptosis. The virus-induced apoptosis was inhibited in cells treated with a human endogenous acid cysteine proteinase inhibitor.
Björklund
H V
HV
Institute of Parasitology, Department of Biology, Abo Akademi University, Turku, Finland. harry.bjorklund@bmc.uu.se
Johansson
T R
TR
Rinne
A
A
D000818:Animals; D017209:Apoptosis; D002347:Carps; D002470:Cell Survival; D002712:Chlorides / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D015891:Cystatins / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D003588:Cytopathogenic Effect, Viral; D053938:DNA Fragmentation; D006801:Humans; D012209:Rhabdoviridae / Q000187:drug effects / Q000502:physiology*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D017967:Zinc Compounds / Q000494:pharmacology; D019287:Zinc Sulfate / Q000494:pharmacology
D002712:Chlorides; D003470:Culture Media; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D015842:Serine Proteinase Inhibitors; D017967:Zinc Compounds; D019287:Zinc Sulfate; C016837:zinc chloride