An anti-chi-1 antibody recognizes a heavily glycosylated protein in rat brain.
The chi(-1) subunit is a recently identified member of a new class of the ionotropic glutamate receptor family that attenuates NMDA receptor current. We have generated a polyclonal C-terminal antibody to the chi(-1) subunit which recognizes a 135-kDa protein in membranes prepared from chi(-1) transfected HEK-293 cells and in rat brain. In the post-natal day 7 (P7) rat brain, Western blot analysis revealed a 135-kDa band in the thalamus and cortex but not the striatum, cerebellum or peripheral tissues. De-glycosylation of the chi(-1) subunit in both transfected cell lines and in the brain reduced the 135-kDa band to 110 kDa, near the predicted molecular weight of the chi(-1) subunit. These studies demonstrate the chi(-1) subunit is expressed as a glycosylated protein subunit in a distribution that parallels that observed for chi(-1) mRNA by in situ hybridization.
Ciabarra
A M
AM
Department of Pharmacology, Yale University School of Medicine and Connecticut Mental Health Center, New Haven 06508, USA.
Sevarino
K A
KA
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002460:Cell Line / Q000378:metabolism; D006031:Glycosylation; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D009419:Nerve Tissue Proteins; D016194:Receptors, N-Methyl-D-Aspartate