title: The 5' flanking regions of IRBP and arrestin have promoter activity in primary embryonic chicken retina cell cultures.
abstract: Primary cultures of embryonic chicken cells from various tissues were transiently transfected with plasmid vectors containing reporter genes linked to a 1.8 kb fragment of the mouse interphotoreceptor retinoid-binding protein (IRBP) 5' flanking region, a 1.5 kb fragment of the mouse arrestin 5' flanking region, or a 3.4 kb sequence of the bovine arrestin 5' flanking region. Promoter activity was evident in retina-derived cells, but not in fibroblasts or cells from whole brain. Transfection response also varied with transfection method, plasmid DNA concentration, post-transfection incubation time, and cell density. The data suggest that the primary embryonic chicken retinal cell culture system is a useful tool in studying photoreceptor-specific gene regulation.
authors.affiliation: Emory Eye Center, Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Boatright
authors.forename: R
authors.initials: R
authors.lastname: Buono
authors.forename: J
authors.initials: J
authors.lastname: Bruno
authors.forename: R K
authors.initials: RK
authors.lastname: Lang
authors.forename: J S
authors.initials: JS
authors.lastname: Si
authors.forename: T
authors.initials: T
authors.lastname: Shinohara
authors.forename: J W
authors.initials: JW
authors.lastname: Peoples
authors.forename: J M
authors.initials: JM
authors.lastname: Nickerson
mesh_terms: D000818:Animals; D019393:Arrestin / Q000235:genetics*; D001921:Brain; D002417:Cattle; D002478:Cells, Cultured; D002642:Chick Embryo; D005136:Eye Proteins; D005347:Fibroblasts; D017930:Genes, Reporter / Q000502:physiology; D051379:Mice; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012160:Retina / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000235:genetics*; D014162:Transfection; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D019393:Arrestin; D005136:Eye Proteins; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial