[The complex detection of AB(O)-system antigens and of keratin polymorphism in the forensic medical study of hair].
Hair proteins were extracted by buffer consisting of 8 M urea, 0.025 M dithiothreitol, 2% sodium dodecylsulfate, and 0.01 M tris-HCl pH 10.4. The proteins were analyzed by gradient polyacrylamide gel electrophoresis. A single 5 cm hair, previously subjected to AB0 blood group analysis by the absorption-elution test, was sufficient for assessing the keratin phenotype. It is possible to determine the AB(0) antigens and detect the keratin phenotype in the same hair fragments.
Kukhar'kov
Iu V
IuV
Puchkov
G F
GF
Borovko
S R
SR
D000017:ABO Blood-Group System / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005554:Forensic Medicine / Q000379:methods; D006197:Hair / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007633:Keratins / Q000032:analysis*; D010641:Phenotype; D011110:Polymorphism, Genetic
D000017:ABO Blood-Group System; D007633:Keratins