Matrix collagen type and pore size influence behaviour of seeded canine chondrocytes.
This study directly compared the behaviour of chondrocytes in porous matrices comprising different collagen types and different pore diameters. There was a dramatic difference in the morphology of the cells in the type I and type II collagen matrices. The cells in the type II collagen matrix retained their chondrocytic morphology and synthesized glycosaminoglycans, while in the type I matrix the chondrocytes displayed a fibroblastic morphology with less biosynthetic activity than those in the type II. Small pore diameter affected morphology initially in the type I matrices and showed a higher increase of DNA content, but with time the cells lost the chondrocytic morphology. Our results demonstrate the marked influence of collagen type and pore characteristics on the phenotypic expression of seeded chondrocytes.
Nehrer
S
S
Department of Orthopedic Surgery, Brigham & Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Breinan
H A
HA
Ramappa
A
A
Young
G
G
Shortkroff
S
S
Louie
L K
LK
Sledge
C B
CB
Yannas
I V
IV
Spector
M
M
D000704:Analysis of Variance; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D003094:Collagen; D004247:DNA / Q000032:analysis; D004285:Dogs; D005109:Extracellular Matrix; D006025:Glycosaminoglycans / Q000032:analysis; D008854:Microscopy, Electron; D011108:Polymers
D006025:Glycosaminoglycans; D011108:Polymers; C058506:collagen-glycosaminoglycan copolymer; D003094:Collagen; D004247:DNA