Decorin and collagen type I gene expression in avian low score normal pectoral muscle.
The avian Low Score Normal (LSN) genetic muscle weakness is phenotypically characterized by a reduction in the ability of the birds to right themselves from a supine position. Compared to normal skeletal muscle, LSN muscle has normal myosin isoform switching and cell-cell recognition, elevated glycosaminoglycan and decorin levels at embryonic Day 20, and a large increase in collagen crosslinking at 6 wk posthatch. To begin to determine the biological mechanism involved in the elevated decorin protein concentration at embryonic Day 20, the steady-state levels of transcripts encoding both decorin and collagen Type I at embryonic Days 14, 19, and 20, and at 1 d and 6 wk posthatch were measured. On embryonic Day 20, collagen Type I transcripts were not different from the control but there was a significant elevation in decorin transcript levels. At 1 d and 6 wk posthatch, transcript levels of decorin and collagen Type I were not different between LSN and controls. The change in decorin transcript steady-state levels is limited to late embryonic development and suggests an alteration in a signal transduction pathway regulating decorin transcription.
Velleman
S G
SG
Department of Animal Sciences, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691, USA.
Coy
C S
CS
D000704:Analysis of Variance; D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D007536:Isomerism; D009123:Muscle Hypotonia / Q000235:genetics / Q000503:physiopathology / Q000662:veterinary*; D009218:Myosins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D010369:Pectoralis Muscles / Q000737:chemistry / Q000196:embryology / Q000378:metabolism*; D010641:Phenotype; D011201:Poultry Diseases / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology; D014158:Transcription, Genetic
D058575:Decorin; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D011509:Proteoglycans; D012333:RNA, Messenger; D003094:Collagen; D009218:Myosins