title: Recognition of contiguous allele-specific peptide elements in the rubella virus E1 envelope protein.
abstract: Peptides which bind to human HLA-DRB1 class II molecules in an allele-specific fashion were derived from the immunodominant E1 envelope protein of rubella virus. Two nonoverlapping E1 peptide epitopes were recognized by rubella virus-specific T cells in the context of independent HLA alleles when presented either separately or as a contiguous polypeptide containing both epitopes. Direct binding analysis of potential peptide epitopes to distinct HLA molecules provides a direct approach for selecting antigenic peptides useful for epitope-based vaccine targeted to multiple HLA types.
authors.affiliation: Virginia Mason Research Center, Seattle, WA 98101, USA.
authors.forename: G T
authors.initials: GT
authors.lastname: Nepom
authors.forename: M E
authors.initials: ME
authors.lastname: Domeier
authors.forename: D
authors.initials: D
authors.lastname: Ou
authors.forename: S
authors.initials: S
authors.lastname: Kovats
authors.forename: L A
authors.initials: LA
authors.lastname: Mitchell
authors.forename: A J
authors.initials: AJ
authors.lastname: Tingle
mesh_terms: D000483:Alleles; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002461:Cell Line, Transformed; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology*; D059811:HLA-DRB1 Chains; D006801:Humans; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis; D012412:Rubella virus / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D016056:Immunodominant Epitopes; D010455:Peptides; D014759:Viral Envelope Proteins; C061826:E1 envelope protein, Rubella virus