Selective incorporation of polyunsaturated fatty acids into organelle phospholipids of animal cells.
The selective incorporation of linoleic (18:2(n-6)) and docosahexaenoic (22:6(n-3)) acids into phospholipids of mitochondria, endoplasmic reticulum, and plasma membrane was investigated by changing the ratio of 22:6(n-3) against 18:2(n-6) in a medium, in which Chinese hamster V79-R cells were grown. In those organelles, 18:2(n-6) and its elongation product (eicosadienoic acid) (20:2 (n-6)) were predominantly incorporated into phosphatidylcholine. However, 22:6(n-3) was incorporated more selectively into phosphatidylethanolamine than 18:2(n-6) and 20:2(n-6).
Masui
H
H
Department of Food and Life Science, Yamaguchi Prefectural University, Japan.
Urade
R
R
Kito
M
M
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D008928:Mitochondria / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism
D005231:Fatty Acids, Unsaturated; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines