title: Membrane topology of the metal-tetracycline/H+ antiporter TetA(K) from Staphylococcus aureus.
abstract: A series of fusions to the reporter proteins alkaline phosphatase and beta-galactosidase have been constructed in the predicted periplasmic and cytoplasmic loops of TetA(K), a protein responsible for efflux-mediated tetracycline resistance in Staphylococcus aureus. The results support a topological model of 14 transmembrane segments for TetA(K).
authors.affiliation: School of Biological Sciences, University of Sydney, New South Wales, Australia.
authors.forename: S L
authors.initials: SL
authors.lastname: Ginn
authors.forename: M H
authors.initials: MH
authors.lastname: Brown
authors.forename: R A
authors.initials: RA
authors.lastname: Skurray
mesh_terms: D000595:Amino Acid Sequence; D017920:Antiporters / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002462:Cell Membrane / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D013211:Staphylococcus aureus / Q000737:chemistry*
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; C079051:tetA protein, Bacteria