[Accumulation of 14C-cystine in inherited cataractous rat lens].
This study was designed to investigate the formation of mixed disulfides of protein and glutathione (GSH) in the cataractous lens. We compared the changes in accumulation of 14C-cystine in cultured inherited cataractous rat lens (ICR/f) during cataractogensis with those in Wistar strain rats. The accumulation of 14C-cystine in water insoluble protein (WIP) of the lens was increased, especially in lens recognized cataract. The radioactivity accumulated in the WIP was released by incubation with 2-mercaptoethanol (2-ME), dithiothereitol (DTT) and GSH. The accumulation of 14C-cystine in WIP was inhibited by pretreatment with DTT. The existence of some materials in the lens-which combined with S-S compounds became clear. A large part of the materials is present in WIP which is increased along with the lens opacification. We surmised that the accumulation of 14C-cystine was related to the reaction of protein-glutathione disulfide (PSSG).
Iijima
T
T
Second Department of Biochemistry, School of Medicine Showa University, Tokyo, Japan.
Takenaka
N
N
Takeda
M
M
Kinohira
Y
Y
Koide
R
R
D000375:Aging / Q000378:metabolism; D000818:Animals; D002386:Cataract / Q000235:genetics* / Q000378:metabolism*; D003459:Crystallins / Q000378:metabolism; D003553:Cystine / Q000378:metabolism*; D003864:Depression, Chemical; D004220:Disulfides / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D003459:Crystallins; D004220:Disulfides; D013439:Sulfhydryl Reagents; D003553:Cystine; D005978:Glutathione; D004229:Dithiothreitol