Polypeptides of hepatitis B surface antigen produced in transgenic potato.
Chimeric genes containing the genomic fragments of hepatitis B virus (HBV) coding for middle (M) and major/small (S) surface proteins were constructed and expressed in transgenic potato plants under the control of the cauliflower mosaic virus 35S promoter. Enzyme-linked immunosorbent assay (ELISA) revealed the presence of HBV surface antigen (HBsAg) determinants in extracts from transgenic plants carrying both constructions, the pre-S2 determinant was identified using the corresponding monoclonal antibodies (mAb) in a dot immunobinding assay. Polypeptides of the immunoaffinity-purified S and M proteins were analyzed by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis. Two polypeptides of the M protein, probably representing different glycosylated forms, and the multimeric forms of HBsAg were observed.
Ehsani
P
P
Molecular Biology Department, Pasteur Institute of Iran, Tehran.
Khabiri
A
A
Domansky
N N
NN
D002678:Chimera; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000235:genetics*; D010446:Peptide Fragments / Q000235:genetics*; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D010446:Peptide Fragments