Functional characterisation of Eskimo dog hemoglobin: II. The interplay of HCO(3)- and Cl-.
Hemoglobin (Hb) from the Eskimo dog (belonging to Canis lupus familiaris) showed similar Bohr effect (delta log P50/delta pH) to human HbA in the presence of 100 mmol l-1 NaCl at 20 degrees C. The presence of 7% carbon dioxide in the desalted condition caused a positive (reversed) Bohr effect in the pH range 7.1-7.5 on Eskimo dog Hb, whereas in human HbA there was no Bohr effect within this pH range. A positive Bohr effect on Eskimo dog Hb in this condition was also observed at 37 degrees C. This could indicate differences in the pK values of the amino terminal residues of the two hemoglobins, with possible pH-dependent binding of both bicarbonate (HCO(3)-) and carbamate. Analysis of the effect of CO2 on oxygen affinity of Eskimo dog Hb in the pH range 6.7-7.6 in the presence of chloride and/or 2,3-diphosphoglycerate (2,3-DPG) support this theory. Our results indicate a competition between HCO(3)- and Cl- in affecting oxygen binding. Thermodynamic analysis reveals that bicarbonate binding lowers the apparent heat of oxygenation in Eskimo dog Hb nearly as much as chloride does in the presence of 2,3-DPG at physiological pH. This safeguards an effective oxygen unloading at lowered red blood cell concentrations of chloride. Moreover, we show that the oxygen affinity at high O2 saturation is less dependent on temperature in the presence than in the absence of CO2-.
Bårdgard
A J
AJ
Institute of Zoology, University of Bergen, Norway. astrid.bardgard@fa.uib.no
Brix
O
O
D000818:Animals; D001639:Bicarbonates / Q000097:blood; D002712:Chlorides / Q000097:blood; D004285:Dogs / Q000097:blood*; D006441:Hemoglobin A / Q000737:chemistry / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D010100:Oxygen / Q000097:blood; D013816:Thermodynamics
D001639:Bicarbonates; D002712:Chlorides; D006454:Hemoglobins; D006441:Hemoglobin A; D010100:Oxygen