Defibrotide augments the anticandidial activity of NK cells.
1. The mechanism of action of Defibrotide, a fibrinolytic agent on Natural Killer (NK) cell cytotoxicity, was investigated through verapamil, TMB-8 cells and pertussis toxin. 2. Defibrotide increased the activity against Candida albicans cells (anticandidial activity), and it is determined that the calcium channels have a role in this effect. 3. Blockage of calcium channels reduced the anticandidial effect by 39.2%. Pertussis toxin led to a 10.7% inhibition, whereas the application of TMB-8 resulted in the stimulation of anticandidial activity. 4. It is concluded that defibrotide is a potent activator of NK cells.
Turhan
A
A
Department of Immunology, Faculty of Medicine, Gazi University, Ankara, Turkey.
Piskin
A K
AK
Emerk
K
K
Imir
T
T
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002176:Candida albicans / Q000254:growth & development* / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin; D011089:Polydeoxyribonucleotides / Q000494:pharmacology*; D013268:Stimulation, Chemical; D014700:Verapamil / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D002121:Calcium Channel Blockers; D005343:Fibrinolytic Agents; D011089:Polydeoxyribonucleotides; D010566:Virulence Factors, Bordetella; C036901:defibrotide; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D014700:Verapamil; D037342:Pertussis Toxin