title: Effect of tumor necrosis factor-alpha on progesterone production by granulosa cells in laying hens of different genetic lines.
abstract: In vitro progesterone production by granulosa cells in the presence or absence of human recombinant tumor necrosis factor-alpha (hrTNF-alpha) was measured at 10, 20, and 30 wk of egg production in White Leghorn hens selected for high (HA)- or low-antibody (LA) response to sheep red blood cell challenge. Isolated granulosa cells from the three largest preovulatory follicles (F1-F3) were incubated with 5 or 250 ng/ml hrTNF-alpha, and progesterone production was determined by the use of a validated radioimmunoassay. F1, F2 and F3 granulosa cells from HA hens produced more (P < or = 0.05) progesterone (140.8, 107.2, and 49.7 ng/ml) than LA hens (109.4, 78.9, and 26.9 ng/ml). The treatment of granulosa cells with hrTNF-alpha consistently inhibited (P < or = 0.05) progesterone secretion by all follicles among HA and LA hens, but not always at both doses. Generally, 5 ng/ml hrTNF-alpha was the maximum inhibitory dose. In the laying hen, a decrease in steroid production in response to cytokines may upset the steroid balance created by follicular hierarchy and inhibit or delay ovulation.
authors.affiliation: Department of Animal Science, University of Tennessee, Knoxville 37901, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Bryan
authors.forename: J M
authors.initials: JM
authors.lastname: Grizzle
authors.forename: A M
authors.initials: AM
authors.lastname: Saxton
authors.forename: P B
authors.initials: PB
authors.lastname: Siegel
authors.forename: E A
authors.initials: EA
authors.lastname: Dunnington
mesh_terms: D000818:Animals; D002470:Cell Survival; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism; D010060:Ovulation; D011374:Progesterone / Q000096:biosynthesis*; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000494:pharmacology; D012641:Selection, Genetic; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D011374:Progesterone