title: Isolation of the mouse (MFH-1) and human (FKHL 14) mesenchyme fork head-1 genes reveals conservation of their gene and protein structures.
abstract: The very recently found evolutionarily conserved DNA-binding domain of 100 amino acids, termed the fork head domain, emerged from a sequence comparison of the rat hepatocyte transcription factor HNF-3 alpha and the homeotic gene fork head of Drosophila. We previously isolated a new member of this family, the mesenchyme fork head-1 (MFH-1) gene, which is expressed in developing mesenchyme. Here we describe the isolation of the mouse (MFH-1) and human (FKHL14) chromosomal MFH-1 genes and the determination of the gene and protein structures of MFH-1. We found that the MFH-1 gene has no introns and that the identity of the amino acid sequences of mouse and human MFH-1 proteins is 94%. We also investigated the transcriptional activity of the mouse and human MFH-1 proteins and found that both proteins act as positive transactivators.
authors.affiliation: Department of Biochemistry, Akita University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Iida
authors.forename: H
authors.initials: H
authors.lastname: Kakinuma
authors.forename: X L
authors.initials: XL
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004330:Drosophila; D019143:Evolution, Molecular; D051858:Forkhead Transcription Factors; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; D009838:Oligodeoxyribonucleotides; D015534:Trans-Activators; D014157:Transcription Factors; C082078:mesenchyme fork head 1 protein