title: DNA binding and cleavage by a cationic manganese porphyrin-peptide nucleic acid conjugate.
abstract: A cationic manganese porphyrin-peptide nucleic acid (PNA) conjugate has been prepared and used to cleave a double-stranded DNA target. Cleavage experiments were performed with a 247-base pair restriction DNA fragment containing a 10-base pair homopurine binding target for the PNA. Oxidative activation by this Mn porphyrin-PNA conjugate leads to sequence specific, 3'-staggered cleavage of both DNA strands near the strand displacement junction. Furthermore, the Mn porphyrin-PNA porphyrin conjugates bind over 100-fold better to double-stranded DNA compared to the native PNA.
authors.affiliation: Laboratoire de Chimie de Coordination du CNRS, Toulouse, France.
authors.forename: P
authors.initials: P
authors.lastname: Bigey
authors.forename: S H
authors.initials: SH
authors.lastname: Sönnichsen
authors.forename: B
authors.initials: B
authors.lastname: Meunier
authors.forename: P E
authors.initials: PE
authors.lastname: Nielsen
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D010084:Oxidation-Reduction; D010455:Peptides / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism*
chemical_list: D010455:Peptides; D011166:Porphyrins; D008345:Manganese; D004247:DNA