title: Deletion in the Z-line region of the titin gene in a baby hamster kidney cell line, BHK-21-Bi.
abstract: The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titin non-functional. These sequences were present in the original BHK-21 line and in hamster DNAs. Our finding shows that, due to the absence of selective pressure on a gene's function, genomic deterioration can occur in a permanent cell line and can lead to a loss of overlapping DNA stretches in both autosomes.
authors.affiliation: Developmental Biology Unit, University of Bielefeld, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Jäckel
authors.forename: C
authors.initials: C
authors.lastname: Witt
authors.forename: O
authors.initials: O
authors.lastname: Antonova
authors.forename: I
authors.initials: I
authors.lastname: Curdt
authors.forename: S
authors.initials: S
authors.lastname: Labeit
authors.forename: H
authors.initials: H
authors.lastname: Jockusch
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D064211:Connectin; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007668:Kidney; D008647:Mesocricetus; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion / Q000235:genetics*
chemical_list: D064211:Connectin; D018076:DNA, Complementary; D009124:Muscle Proteins; C577285:TTN protein, human; D011494:Protein Kinases; D015246:Deoxyribonuclease EcoRI