Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg(2+)-contaminated environments.
Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein significantly increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent properties for bioremediation of Hg(2+)-contaminated environments.
Chen
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Institute for Comparative and Environmental Toxicology, Cornell University, Ithaca, New York 14853, USA.
Wilson
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DB
D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D027682:Cation Transport Proteins; D004352:Drug Resistance, Microbial / Q000235:genetics; D004785:Environmental Pollutants / Q000493:pharmacokinetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D005818:Genetic Engineering; D005982:Glutathione Transferase / Q000235:genetics; D017136:Ion Transport / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D008628:Mercury / Q000493:pharmacokinetics*; D008668:Metallothionein / Q000235:genetics; D018532:Pisum sativum / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011506:Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D004785:Environmental Pollutants; D008565:Membrane Proteins; C086060:MerP protein, Bacteria; D011506:Proteins; D011993:Recombinant Fusion Proteins; C094115:mercury transport protein, bacterial; D008668:Metallothionein; D005982:Glutathione Transferase; D008628:Mercury