Detection of mumps virus genome directly from clinical samples and a simple method for genetic differentiation of the Hoshino vaccine strain from wild strains of mumps virus.
A simple and sensitive method was developed for the differentiation of the Hoshino vaccine strain from wild strains with a restriction fragment length polymorphism (RFLP) analysis in the part of hemagglutinin-neuraminidase (HN) gene. The virus genome was amplified by using a reverse transcriptase-polymerase chain reaction (RT-PCR) directly from clinical samples. The PCR product of the Hoshino vaccine strain was cleaved into 2 fragments after digestion with Sca I and Afl II. All wild strains showed 2 RFLP profiles, A and B, different from that of vaccine strain. Wild A strains were cut into 2 fragments after digestion with Sca I only, while wild B strains were cleaved neither with Sca I nor Afl II. This molecular approach provides an effective method for differentiation of the Hoshino vaccine strain from wild strains of mumps virus in patients after vaccination.
Kashiwagi
Y
Y
Department of Pediatrics, Tokyo Medical College, Japan.
Kawashima
H
H
Takekuma
K
K
Hoshika
A
A
Mori
T
T
Nakayama
T
T
D000818:Animals; D002522:Chlorocebus aethiops; D016679:Genome, Viral; D015707:HN Protein / Q000235:genetics*; D006801:Humans; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid / Q000821:virology; D009107:Mumps / Q000473:pathology / Q000821:virology*; D009108:Mumps Vaccine / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009109:Mumps virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D014709:Vero Cells
D015707:HN Protein; D009108:Mumps Vaccine