Dot-immunogold-silver staining in the diagnosis of cysticercosis.
Dot-immunogold-silver staining (Dot-IGSS) and Dot-ELISA were used, with Taenia solium cyst fluid antigen, to detect specific antibodies in the sera of patients with cysticercosis. All 40 confirmed cases of cysticercosis were found to be positive when tested by 2 methods. The average titer of the sera detected by Dot-IGSS was 1:27,470, which was significantly higher than that detected by Dot-ELISA. The false positive rates were 2% and 4% in 50 healthy controls for Dot-IGSS and Dot-ELISA, respectively, with a low titer of 1:20.
Liu
Y S
YS
Department of Physiology, Xuzhou Medical College, People's Republic of China.
Du
W P
WP
Wu
Z X
ZX
D000909:Antibodies, Helminth / Q000097:blood*; D000918:Antibody Specificity; D003551:Cysticercosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017979:Gold Colloid; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G; D008297:Male; D009942:Organometallic Compounds; D016622:Silver Staining
D000909:Antibodies, Helminth; D017979:Gold Colloid; D007074:Immunoglobulin G; D009942:Organometallic Compounds