[Effect of hypochlorite and hydrogen peroxide on the ability of hemoglobin to stimulate lipid peroxidation of low density lipoproteins].
The ability of hemoglobin, modified by H2O2 or HOCl/OCl-, to induce lipid peroxidation (LPO) in low density lipoproteins (LDL) was studied, as well as the effects of haptoglobin. It was found that Hb modification by H2O2 or HOCl/OCl- increased generation of TBA-reactive substances in low density lipoproteins. Modified Hb was as double or more reactive compared to intact Hb. Free radical scavengers (ethanol and mannitol) gave no effect on LPO in LDL. On the other hand, ferric iron chelator desferrioxamine decreased LPO 5-6 times. Ferrous iron chelator- o-phenanthroline was effective only in the case of LPO, induced by H2O2 modified Hb. Haptoglobin (plasma protein forming complexes with Hb) decreased LPO induced by both intact or HOCl/OCl modified Hb. The results of the paper show that modification of Hb by H2O2 or HOCl/OCl- increase the ability of Hb to induce LPO in LDL, probably due to metHb, ferrylHb or free iron production.
Osipov
A N
AN
Briukhanova
E V
EV
Vakhrusheva
T V
TV
Panasenko
O M
OM
Vladimirov
Iu A
IuA
D016166:Free Radical Scavengers; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006997:Hypochlorous Acid / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000494:pharmacology; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010618:Phenanthrolines / Q000494:pharmacology
D016166:Free Radical Scavengers; D006454:Hemoglobins; D007502:Iron Chelating Agents; D008077:Lipoproteins, LDL; D010618:Phenanthrolines; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide; C025205:1,10-phenanthroline