Protection in a gerbil model of amebiasis by oral immunization with Salmonella expressing the galactose/N-acetyl D-galactosamine inhibitable lectin of Entamoeba histolytica.
Infection with the enteric parasite Entamoeba histolytica can result in colitis and dysentery as well as abscesses at extra-intestinal sites. An effective vaccine must be able to protect against both mucosal and systemic disease. In this study an attenuated Salmonella strain that expressed a portion of the GalNAc lectin of E, histolytica was used to orally immunize gerbils. Animals were challenged by intrahepatic injection of amebic trophozoites. A significant decrease in size of amebic liver abscesses was observed in orally immunized animals. Oral immunization with a Salmonella-based vaccine was as effective as systemic immunization for protection against systemic challenge.
Mann
B J
BJ
Department of Internal Medicine, University of Virginia, School of Medicine, Charlottesville 22908, USA.
Burkholder
B V
BV
Lockhart
L A
LA
D000284:Administration, Oral; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004195:Disease Models, Animal; D004748:Entamoeba histolytica / Q000276:immunology*; D005822:Genetic Vectors; D005849:Gerbillinae; D007114:Immunization; D007274:Injections, Intraperitoneal; D037102:Lectins; D008101:Liver Abscess, Amebic / Q000276:immunology / Q000517:prevention & control*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D012475:Salmonella / Q000235:genetics* / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D000913:Antibodies, Protozoan; D037102:Lectins; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic; C052452:galactose inhibitable adherence protein, Entamoeba histolytica