Force and myosin content variation in isolated intact single muscle fibres from Rana temporaria.
We studied the relation between force normalized by dry mass per unit length and the myosin fraction of muscle dry mass. The two tibialis anterior muscles were dissected from 12 frogs (Rana temporaria). Then, from one muscle, two single fast-twitch fibres were isolated. Each fibre was mounted isometrically in Ringer's solution, and electrically stimulated using a standardized protocol. Peak force production, normalized by the fibre's dry mass per unit length, varied by a factor of 1.4. Little variation in normalized force was measured between fibres from the same animal, whereas between animals a significant difference was found (P<0.05). The contralateral muscle was used to determine the myosin fraction of the dry mass. The relationship between the fraction myosin of the dry mass and force normalized by dry mass per unit length showed a high correlation (r = 0.81; n = 12). From this we conclude that variation in normalized tetanic force is determined greatly (65%) by variations in myosin content.
Buschman
H P
HP
Laboratory for Physiology, Institute for Cardiovascular Research, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands.
van der Laarse
W J
WJ
Stienen
G J
GJ
Elzinga
G
G
D000818:Animals; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000502:physiology*; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism / Q000502:physiology*; D009218:Myosins / Q000378:metabolism*; D011896:Rana temporaria / Q000378:metabolism / Q000502:physiology*
D009218:Myosins