Effect of ethanol on nuclear casein kinase II activity in brain.
Casein kinase II (CK II) plays an important role in serine/threonine dependent protein phosphorylation. In brain it is associated with long term potentiation besides its involvement in DNA, RNA and protein metabolism. Ethanol has been shown to induce cognitive impairment and affects DNA, RNA and protein metabolism at various steps. Since CK II is central in all these events, which are specifically affected by ethanol, the role of nuclear CK II is investigated in the present study. Total nuclear casein kinase activity was unaffected while heparin sensitive nuclear casein kinase II activity showed a 30% decrease in the brain from chronic alcohol fed rats. Cytosolic CK II activity was also unaffected. Immunological detection by western analysis using CK II antibodies showed no alteration in the quantity of enzyme. The decrease in nuclear casein kinase II might be responsible for ethanol induced cognitive impairment in the brain.
Haviryaji
K S
KS
School of Life Sciences, University of Hyderabad, India. mcvsl@uohyd.ernet.in
Vemuri
M C
MC
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D047390:Casein Kinase II; D002467:Cell Nucleus / Q000187:drug effects* / Q000201:enzymology; D003072:Cognition Disorders / Q000139:chemically induced* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D008970:Molecular Weight; D017346:Protein Serine-Threonine Kinases / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D004268:DNA-Binding Proteins; D000431:Ethanol; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases