Growth inhibition, morphological change, and ectoenzyme release of LLC-PK1 cells by phosphatidylinositol-specific phospholipase C of Bacillus thuringiensis.
Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus thuringiensis added to a culture of LLC-PK1 cells inhibited cell growth by 40%. In contrast with normal cells, the cells cultured in the presence of PI-PLC showed needle-like appendages which seemed to have been formed due to portions of the cell remaining adhered to the culture dish as the cell shrank. When LLC-PK1 cells were treated with PI-PLC, significant amounts of alkaline phosphatase and alkaline phosphodiesterase I were released specifically from the apical surface of the LLC-PK1 cells. Furthermore, PI-PLC treatment caused a delay of enzyme production and dome formation. These data indicate that glycosyl-phosphatidylinositol (GPI)-anchored proteins on the surface of LLC-PK1 cells are important in cell growth and differentiation. Also, the combined use of LLC-PK1 cells and PI-PLC of B. thuringiensis is effective for investigating the function of GPI-anchor proteins.
Itami
C
C
Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
Kimura
Y
Y
Taguchi
R
R
Ikezawa
H
H
Nakabayashi
T
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001413:Bacillus thuringiensis / Q000201:enzymology*; D002455:Cell Division / Q000187:drug effects; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D006131:Growth Inhibitors / Q000494:pharmacology*; D018374:LLC-PK1 Cells; D008970:Molecular Weight; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D043264:Phosphodiesterase I; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013552:Swine; D010738:Type C Phospholipases / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D006131:Growth Inhibitors; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D010738:Type C Phospholipases; D043264:Phosphodiesterase I; D054801:Phosphoinositide Phospholipase C; D018819:Dipeptidyl Peptidase 4; D043265:Phosphatidylinositol Diacylglycerol-Lyase