Restricted TCR V alpha repertoire in the T cell response to a tolerogenic determinant of type II collagen.
Tolerization of B10.RIII mice (H-2r) with i.v.-injected type II collagen (CII) renders the animals resistant to induction of collagen-induced arthritis (CIA). The B10.RIII mouse is of particular interest, in that the T cell determinants that induce tolerance are different from those that induce arthritis. To characterize T cells that react with the tolerogenic determinant and play a role in regulation of arthritis, we have developed a panel of T cell hybridomas reactive with the tolerogenic T cell epitope, CII 607-621. None of the hybrids cross-reacted with either the arthritogenic CII 445-453 or murine CII. As determined by PCR and immunofluorescence, the T cell response to the tolerogenic determinant was oligoclonal, with evident preferential usage of V alpha. Through the analysis of a large panel of T cell hybridomas, preferential usage of V alpha2, J alpha44, J beta2.7, and D beta2.1 was observed. Characterization of T cells reactive with the immunodominant determinant, CII 607-621, responsible for the induction of tolerance should prove important in developing novel therapeutic approaches for the treatment of autoimmune diseases.
Kawamura
S
S
Department of Pediatrics, University of Tennessee, Memphis 38163, USA.
Miyahara
H
H
Esaki
Y
Y
Myers
L K
LK
Rosloniec
E F
EF
Stuart
J M
JM
Kang
A H
AH
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology; D001168:Arthritis / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology; D001483:Base Sequence; D003094:Collagen / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007108:Immune Tolerance; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D000939:Epitopes; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015847:Interleukin-4; D003094:Collagen