title: Quantitation of vaginally administered nonoxynol-9 in premenopausal women.
abstract: A feasibility study was performed in 11 healthy nonpregnant premenopausal women to determine a method for collection and recovery of vaginally administered nonoxynol-9. We also determined if nonoxynol-9 could be quantitated in vaginal lavage fluid obtained 2 h after instillation of a standard precoitol dose of a foam formulation of nonoxynol-9. Samples were analyzed in batch using a validated normal phase high-performance liquid chromatography (HPLC) method. Two hours after instillation of one dose of Delfen Contraceptive Foam (100 mg), the quantity of nonoxynol-9 collected ranged from 10.8 to 67.8 mg (mean: 35.4 mg). This corresponds to a recovery of 11.70%, of the administered dose. Quantitation of vaginally administered nonoxynol-9 is both practical and feasible. These data represent a critical first step in the evaluation of the safety and effectiveness of nonoxynol-9-containing products in the prevention of sexually transmitted diseases.
authors.affiliation: Department of Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.
authors.forename: P
authors.initials: P
authors.lastname: Barditch-Crovo
authors.forename: F
authors.initials: F
authors.lastname: Witter
authors.forename: F
authors.initials: F
authors.lastname: Hamzeh
authors.forename: J
authors.initials: J
authors.lastname: McPherson
authors.forename: P
authors.initials: P
authors.lastname: Stratton
authors.forename: N J
authors.initials: NJ
authors.lastname: Alexander
authors.forename: C B
authors.initials: CB
authors.lastname: Trapnell
mesh_terms: D000282:Administration, Intravaginal; D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D017137:Nonoxynol / Q000008:administration & dosage / Q000032:analysis*; D017697:Premenopause; D013089:Spermatocidal Agents / Q000008:administration & dosage / Q000032:analysis*; D007507:Therapeutic Irrigation; D014621:Vagina / Q000378:metabolism*
chemical_list: D013089:Spermatocidal Agents; D017137:Nonoxynol