[The activity of a number of anabolic metabolism enzymes in Staphylococcus aureus strains containing plasmids of antibiotic resistance].
Activity of a number of enzymes participating in the processes of structure metabolism and dynamics of inclusion of 14C-aspartate in initial strain of Staphylococcus aureus sensitive to antibiotics and strains containing plasmids of resistance to various antibiotics have been comparatively studied. An increase in activity of fumarate-hydratase, acetyl-KoA-carboxylase, aspartate aminotransferase and well as true increases (by the rate of inclusion of 14C-aspartate in a number of strains containing plasmids) of resistance to antibiotics as compared to plasmid-less variant are shown. This evidence for strengthening of biosynthetic function of a cycle of tricarbonic acids and, allowing for extremely low activity of alpha-ketoglutarate dehydrogenase, one can suppose that an open cycle of tricarbonic acids functions in the given strain of staphylococcus. The increase of the level of acetyl-KoA-carboxylase activity evidences for the intensification of lipid synthesis in antibiotic-resistant staphylococci as compared to the sensitive variants.
Kozitskaia
S N
SN
Gavriliuk
V G
VG
Golodok
L P
LP
Vinnikov
A I
AI
D001224:Aspartic Acid / Q000378:metabolism; D002250:Carbon Radioisotopes; D002952:Citric Acid Cycle; D004352:Drug Resistance, Microbial / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics
D002250:Carbon Radioisotopes; D001224:Aspartic Acid