Identification of pore-forming subunit of P-type calcium channels: an antisense study on rat cerebellar Purkinje cells in culture.
Treatment of cerebellar neurones in culture with an antisense oligonucleotide (ODN) against alpha1A, reduced the whole-cell P-type calcium channel current relative to mismatch ODN treated controls (p < 0.001). Therefore, AgaIVA (50 nM) reduced whole-cell calcium current in mismatch and antisense treated cells by 70 +/- 4 and 19 +/- 3%, respectively.
Gillard
S E
SE
Lilly Research Centre, Eli Lilly Co. Ltd., Windlesham, Surrey, U.K.
Volsen
S G
SG
Smith
W
W
Beattie
R E
RE
Bleakman
D
D
Lodge
D
D
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D002531:Cerebellum / Q000187:drug effects*; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011247:Pregnancy; D011689:Purkinje Cells / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D015220:Calcium Channels; D016376:Oligonucleotides, Antisense