title: NGF and BDNF increase the immunoreactivity of vesicular acetylcholine transporter in cultured neurons from the embryonic rat septum.
abstract: The expression of vesicular acetylcholine transporter (VAChT), which transports ACh into synaptic vesicles, is coregulated with choline acetyltransferase (ChAT). Therefore, the effects of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) on the levels of VAChT in cultured neurons from the septum of embryonic rats were investigated by immunocytochemistry. NGF and BDNF increased the number of VAChT-immunoreactive neurons by approximately 1.5-fold and enhanced the immunoreactivity in each positive cell. These results suggest that the neurotrophins enhance not only synthesis but also storage of ACh in septal neurons.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Sakyo, Japan. nobu.takei@mun.uu.se
authors.forename: N
authors.initials: N
authors.lastname: Takei
authors.forename: H
authors.initials: H
authors.lastname: Kuramoto
authors.forename: Y
authors.initials: Y
authors.lastname: Endo
authors.forename: H
authors.initials: H
authors.lastname: Hatanaka
mesh_terms: D000109:Acetylcholine; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002352:Carrier Proteins / Q000032:analysis*; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D007150:Immunohistochemistry; D026901:Membrane Transport Proteins; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D012688:Septum Pellucidum / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D009414:Nerve Growth Factors; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D000109:Acetylcholine