Role of transforming growth factor-beta 1 and -beta 2 in ddY mouse nephropathy.
We investigated the glomerular distribution of transforming growth factor-beta (TGF-beta 1 and TGF-beta 2) protein and the expression of its mRNA, and related factors, in ddY mice, aged 5-60 weeks, before and after the onset of nephropathy, TGF-beta 1 protein expression was observed from the age of 20 weeks onwards, peaking at 50 weeks, and then declining. Expression of TGF-beta 2 protein gradually increased from 5 to 60 weeks. TGF-beta 1 and TGF-beta 2 mRNA were both detected from 5 to 60 weeks. The mesangial matrix expansion index (MMEI) was significantly higher in mice with nephropathy than in those without nephropathy, as was the expression of TGF-beta 1 and TGF-beta 2 proteins (P < 0.05). TGF-beta 2 was significantly positively correlated with the MMEI (P < 0.05). Infiltration of CD68-positive monocytes/macrophages gradually increased until 60 weeks, and was significantly correlated with the expression of TGF-beta 1 (P < 0.05) and TGF-beta 2 (P < 0.01). These findings indicate that TGF-beta 1 and TGF-beta 2 were overexpressed in ddY mice with overt nephropathy compared with pre-nephropathic mice. TGF-beta 2 may be an important mediator of mesangial matrix expansion in ddY mouse nephropathy.
Ye
X F
XF
Second Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Yorioka
N
N
Oda
H
H
Taniguchi
Y
Y
Yamakido
M
M
D000375:Aging / Q000235:genetics / Q000276:immunology; D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D001483:Base Sequence; D015331:Cohort Studies; D003404:Creatinine / Q000097:blood; D017931:DNA Primers / Q000737:chemistry; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000502:physiology; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression / Q000235:genetics; D005920:Glomerular Mesangium / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D005922:Glomerulonephritis, IGA / Q000503:physiopathology*; D007106:Immune Sera / Q000276:immunology; D007678:Kidney Glomerulus / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009000:Monocytes / Q000276:immunology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D015703:Antigens, CD; D017931:DNA Primers; D007106:Immune Sera; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D003404:Creatinine