CKbeta8, a novel CC chemokine that predominantly acts on monocytes.
We have studied the biological properties of a new human CC chemokine, CKbeta8, consisting of 99 amino acids including six cysteines. CKbeta8 mRNA transcripts were induced in monocytes by IL-1beta and, to a lesser extent, by IFNgamma, and were detected in RNA extracted from normal human liver and gastrointestinal tract. CKbeta8 is chemotactic for monocytes, but is inactive on IL-2 conditioned T lymphocytes, eosinophils and neutrophils. Desensitization experiments indicate that CKbeta8 and MIP-1beta completely share receptors on monocytes and that the CKbeta8 receptor, which appears to differ from the known ones, is also recognized by MCP-1, MCP-2, MCP-3, MCP-4, MIP-1alpha and RANTES.
Forssmann
U
U
Theodor Kocher Institute, University of Bern, Switzerland.
Delgado
M B
MB
Uguccioni
M
M
Loetscher
P
P
Garotta
G
G
Baggiolini
M
M
D000118:Acetylglucosaminidase / Q000378:metabolism; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D018925:Chemokines / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D019742:Chemokines, CC; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D003571:Cytochalasin B / Q000494:pharmacology; D004804:Eosinophils / Q000187:drug effects; D005456:Fluorescent Dyes / Q000378:metabolism; D016257:Fura-2 / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000502:physiology*; D018121:Receptors, Cytokine / Q000378:metabolism; D016415:Sequence Alignment; D017421:Sequence Analysis; D013601:T-Lymphocytes / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
C105504:CCL23 protein, human; D018925:Chemokines; D019742:Chemokines, CC; D005456:Fluorescent Dyes; D007376:Interleukin-2; D018121:Receptors, Cytokine; D010566:Virulence Factors, Bordetella; D003571:Cytochalasin B; D000118:Acetylglucosaminidase; D002118:Calcium; D016257:Fura-2