title: Mutational analysis and genetic cloning of the agnostic locus, which regulates learning ability in Drosophila.
abstract: P-insertion mutations were obtained and localized by in situ methods at the agnostic gene (agn: 1-38.9; 11AB) in Drosophila. All agn mutants showed a wide spectrum of pleiotropic effects: an EMS-induced mutation of the agn-ts398 improved the ability to develop a conditioned defensive response and increased the activity of cAMP metabolic enzymes; spontaneous mutation of agnX1 showed morphological defects of the brain. P-insertion mutations were used to clone the gene; a restriction map of 80 kb in length was determined, and the insertion was localized to a fragment of 9 kb.
authors.affiliation: Laboratory of Comparative Behavioral Genetics, I.P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg.
authors.forename: A I
authors.initials: AI
authors.lastname: Peresleni
authors.forename: E V
authors.initials: EV
authors.lastname: Savvateeva
authors.forename: I V
authors.initials: IV
authors.lastname: Peresleni
authors.forename: L M
authors.initials: LM
authors.lastname: Sharagina
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D004252:DNA Mutational Analysis; D004330:Drosophila; D005260:Female; D017344:Genes, Insect / Q000235:genetics*; D017403:In Situ Hybridization; D007858:Learning / Q000502:physiology*; D008297:Male; D016254:Mutagenesis, Insertional; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D014960:X Chromosome / Q000502:physiology / Q000648:ultrastructure
chemical_list: D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases