Distribution of endosomal, lysosomal, and major histocompatability complex markers in a monocytic cell line infected with Chlamydia psittaci.
The intracellular fate of Chlamydia psittaci during infection of a monocytic cell line, THP1, was characterized. Cytochalasin D inhibited phagocytosis of latex beads but had no effect on infection by C. psittaci, and vacuoles expressed the transferrin receptor, suggesting accessibility to the endocytic pathway. Early Chlamydia-containing vacuoles expressed major histocompatibility complex (MHC) class I molecules, and most vacuoles fused with host cell lysosomes, since they expressed LAMP-1 and had acidic pHs. In cells prestimulated with gamma interferon, vacuoles also expressed MHC class II molecules, suggesting that the monocytes might effectively process Chlamydia-derived antigens for presentation by MHC class I and class II molecules.
Ojcius
D M
DM
Unité de Biologie des Interactions Cellulaires, CNRS URA 1960, Institut Pasteur, Paris, France. ojcius@pasteur.fr
Hellio
R
R
Dautry-Varsat
A
A
D000818:Animals; D002691:Chlamydophila psittaci / Q000276:immunology*; D011992:Endosomes / Q000378:metabolism*; D006168:Guinea Pigs; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis; D014407:Tumor Cells, Cultured; D014617:Vacuoles / Q000382:microbiology
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II