Ultrastructure of murine dermis following topical application of the vitamin D3 analogue KH-1060.
The aim of this study, was to observe the effects of KH-1060, a new vitamin D3 analogue, on the dermis of hairless mice by electron microscopy. KH-1060 (0.2 micrograms/ml in isopropanol) or KH-1060 following betamethasone 17-valerate (2 mg/ml in isopropanol) was applied topically to the backs of hairless mice for 4 weeks. KH-1060 increased the number of dermal fibroblasts, the cytoplasm of which was dominated by secretory components. Mast cells contained normal mature granules and degranulated after disintegration. No extrusion of non-disintegrated granules was seen. Collagen fibrils were thickened and increased in number; however the content of type I collagen in the fibrils did not increase. Glycosaminoglycan figures appeared distinct. KH-1060 prevents betamethasone-induced changes in collagen fibrils and glycosaminoglycans, while no prevention was seen for mast cells.
Kobayasi
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Department of Dermatology, University of Copenhagen, Rigshospital, Denmark.
D000818:Animals; D001623:Betamethasone / Q000494:pharmacology; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000494:pharmacology; D003094:Collagen / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D006025:Glycosaminoglycans / Q000648:ultrastructure; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D051379:Mice; D008812:Mice, Hairless; D008854:Microscopy, Electron; D012867:Skin / Q000187:drug effects* / Q000648:ultrastructure
D006025:Glycosaminoglycans; D007166:Immunosuppressive Agents; C074279:KH 1060; D003094:Collagen; D001623:Betamethasone; D002117:Calcitriol