PCR strategy to obtain the 5' and 3' flanking sequences of partial clones from lambda ZAP cDNA libraries.
Li
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University of North Carolina at Chapel Hill, USA.
Nicholas
R A
RA
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D019204:GTP-Binding Proteins / Q000235:genetics; D015723:Gene Library; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D018048:Receptors, Purinergic P2 / Q000235:genetics; D014422:Turkeys
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D019204:GTP-Binding Proteins