[Detection of dermonecrotic toxin genes in Pasteurella multocida strains using the polymerase chain reaction (PCR)].
A PCR method was developed which allows to distinguish between Pasteurella multocida strains carrying or lacking the dermonecrotic toxin gene. Specific primers were used to amplify a 1501-bp DNA fragment from the genomic dermonecrotic toxin gene region. Isolated DNA, broth cultures and swabs were used as samples. Detection of the toxin gene directly from swab samples accelerates considerably the diagnosis since cultivation steps can be omitted. The results of PCR corresponded to findings obtained by ELISA.
Hotzel
H
H
Institut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Jena.
Erler
W
W
Schimmel
D
D
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics*; D002417:Cattle; D017931:DNA Primers; D003885:Dermotoxins / Q000235:genetics*; D016979:Pasteurella multocida / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000379:methods; D013552:Swine
D001427:Bacterial Toxins; D017931:DNA Primers; D003885:Dermotoxins; C052804:dermonecrotic toxin, Pasteurella multocida