The transcription of liver thioredoxin following the ionizing irradiation of radioresistant and radiosensitive mice.
The radiation protective effect of thioredoxin (TRX) in a bacterial system has been reported and based upon this observation we were interested to examine TRX transcription in the mammalian system following ionizing irradiation. In order to answer the question whether radiation sensitive mice (BALB/c) showed TRX transcription different from radiation resistant mice (C3H), we exposed these strains to X-ray doses of 2 Gy, 4 Gy and 6 Gy. Groups consisting of 6 mice were sacrificed 5, 15 and 30 minutes after irradiation and livers were immediately taken into liquid nitrogen. Total RNA was isolated from the organs by the use of a commercially available kit and used for Northern blots and slot blots with a chemiluminescence technique. Northern blots revealed a single band at 538 bp for TRX and at 1.8 kb for beta-actin. Quantification of mRNA TRX by densitometry of slot blots revealed that C3H transcribed TRX significantly higher at an earlier time point (5 min) than BALB/c. This delayed transcription of TRX in the radiosensitive mouse strain showed a comparable pattern at three different radiation doses and may well be responsible for radioresistance although no quantitative differences of TRX transcription between BALB/c and C3H mice were detectable.
Hardmeier
R
R
Department of Pediatrics, University of Vienna, Austria.
Rink
H
H
Hoeger
H
H
Labudova
O
O
Lubec
G
G
D000199:Actins / Q000737:chemistry / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D008099:Liver / Q000737:chemistry / Q000528:radiation effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000737:chemistry / Q000528:radiation effects; D011836:Radiation Tolerance / Q000235:genetics*; D013879:Thioredoxins / Q000235:genetics* / Q000528:radiation effects*; D013997:Time Factors; D014158:Transcription, Genetic / Q000528:radiation effects*
D000199:Actins; D012333:RNA, Messenger; D013879:Thioredoxins