title: Expression of intercellular adhesion molecule-1 and lymphocytefunction-associated antigen-1 in experimental Schistosoma mansoni infection and in synchronous periparticular hepatic granulomas in mice: immunohistochemistry, confocal laser scanning microscopy, and immunoelectron microscopy.
abstract: Adhesion molecules play an important role in inflammatory and immunological responses. We assessed the expression pattern of intercellular adhesion molecule-1 (ICAM-1) and lymphocytefunction-associated antigen-1 (LFA-1) in the livers of mice experimentally infected with Schistosoma mansoni and in synchronous hepatic granulomas induced by injection of soluble egg antigen (SEA)-coupled Sepharose beads in a mesenteric vein of mice. By immunohistochemistry, confocal laser scanning microscopy, and immunoelectron microscopy, ICAM-1 was localized on endothelial cells, sinusoidal-lining cells (Kupffer cells and sinusoidal endothelium), the hepatocyte cell membrane facing Disse's space, and inflammatory cells in the granuloma. LFA-1 was visualized on the inflammatory cells of the granuloma and on phagocytic sinusoidal-lining cells, most likely Kupffer cells. ICAM-1- and LFA-1-immunoreactive cells were present in the granuloma as early as at 3 days after injection of SEA-coupled beads and persisted with time. As granulomas became older, nonimmunoreactive granuloma cells appeared. We conclude that adhesion molecules play an important role in the genesis of the schistosomal granuloma.
authors.affiliation: Laboratory of Pathology, Universitaire Instelling Antwerpen, Wilrijk, Belgium.
authors.forename: W
authors.initials: W
authors.lastname: Jacobs
authors.forename: J
authors.initials: J
authors.lastname: Bogers
authors.forename: A
authors.initials: A
authors.lastname: Deelder
authors.forename: E
authors.initials: E
authors.lastname: Van Marck
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006099:Granuloma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D008099:Liver / Q000737:chemistry* / Q000473:pathology; D008109:Liver Diseases, Parasitic / Q000378:metabolism* / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis*; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D012555:Schistosomiasis mansoni / Q000378:metabolism* / Q000473:pathology
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1