Targeted expression of Cre recombinase to adipose tissue of transgenic mice directs adipose-specific excision of loxP-flanked gene segments.
Functional analysis of mammalian genes relies, in part, on targeted mutations generated by homologous recombination in mice. We have developed a strategy for adipose-specific inactivation of loxP-floxed gene segments. Transgenic mice have been established that express Cre recombinase under the control of the adipose-specific aP2 enhancer/promoter. Crossing of the aP2/ Cre mice with any loxP-floxed gene will facilitate its functional analysis in adipose tissue.
Barlow
C
C
Laboratory of Genetic Disease Research, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. cbarlow@nhgri.nih.gov
Schroeder
M
M
Lekstrom-Himes
J
J
Kylefjord
H
H
Deng
C X
CX
Wynshaw-Boris
A
A
Spiegelman
B M
BM
Xanthopoulos
K G
KG
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D003433:Crosses, Genetic; D004742:Enhancer Elements, Genetic; D005821:Genetic Techniques; D019426:Integrases / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013234:Stem Cells; D014764:Viral Proteins
D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases