title: Function of the Greek key connection analysed using circular permutants of superoxide dismutase.
abstract: Human Cu,Zn superoxide dismutase (SOD) is a single domain all beta-sheet protein with its eight beta-strands arranged as a Greek key beta-barrel or immunoglobulin fold. Three circularly permuted variants of SOD were made by joining the native amino- and carboxy-termini, and introducing new termini at sites originally within connections between beta-strands. The locations of the new termini were chosen to interrupt beta-turns between the two N-terminal beta-hairpins and the short cross-barrel Greek key connection. Expression levels in the Escherichia coli periplasm were indistinguishable from that of native SOD. Reaction rates for the purified proteins were similar to those of the native enzyme, indicating that the permutants are correctly folded. Interrupting the covalent cross-bracing provided by the Greek key connection reduced the stability of the protein by approximately 1.0 kcal/mol, indicating only a slight contribution to conformational stability. The experiments test and eliminate two hypotheses for folding pathways for Greek key beta-barrels that require N-terminal beta-hairpins or covalent attachment across the short Greek key connection.
authors.affiliation: Molecular Biology Department, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: M
authors.initials: M
authors.lastname: Boissinot
authors.forename: S
authors.initials: S
authors.lastname: Karnas
authors.forename: J R
authors.initials: JR
authors.lastname: Lepock
authors.forename: D E
authors.initials: DE
authors.lastname: Cabelli
authors.forename: J A
authors.initials: JA
authors.lastname: Tainer
authors.forename: E D
authors.initials: ED
authors.lastname: Getzoff
authors.forename: R A
authors.initials: RA
authors.lastname: Hallewell
mesh_terms: D000595:Amino Acid Sequence; D002152:Calorimetry, Differential Scanning; D004795:Enzyme Stability; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D013482:Superoxide Dismutase