title: Immune responses to individual rotavirus proteins following heterologous and homologous rotavirus infection in mice.
abstract: Serum and intestinal humoral immune responses to rotavirus proteins VP2, VP4, VP6, VP7, NSP2, and NSP4 were quantitatively compared in mice infected with a homologous murine rotavirus (EHPw) or a heterologous simian rotavirus (RRV). Viral protein-specific antibody responses were measured by an immunohistochemistry assay that uses recombinant baculovirus-expressed rotavirus proteins as antigens. In serum, IgG responses to VP6 were dominant and comparable in both RRV- and EHPw-infected groups, but responses to VP2, VP4, VP7, and NSP2 were higher in RRV infection. In feces, IgA responses to VP2, VP4, and VP6 were higher in EHPw-infected mice, but responses to VP7 and NSP2 were detected only in the RRV-infected group. These findings indicate that immune responses to homologous and heterologous rotavirus infection vary both quantitatively and qualitatively. Differences in humoral responses may play a role in the differences in protection induced following homologous or heterologous rotavirus infection.
authors.affiliation: Department of Medicine, Stanford University School of Medicine, California 94305-5487, USA.
authors.forename: S I
authors.initials: SI
authors.lastname: Ishida
authors.forename: N
authors.initials: N
authors.lastname: Feng
authors.forename: J M
authors.initials: JM
authors.lastname: Gilbert
authors.forename: B
authors.initials: B
authors.lastname: Tang
authors.forename: H B
authors.initials: HB
authors.lastname: Greenberg
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D000956:Antigens, Viral; D002460:Cell Line; D005243:Feces / Q000737:chemistry; D005260:Female; D000882:Haplorhini; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology*; D013045:Species Specificity; D018411:Spodoptera; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014764:Viral Proteins