Isolation of an anaerobic bacterium which reductively dechlorinates tetrachloroethene and trichloroethene.
Strain TEA, a strictly anaerobic, motile rod with one to four lateral flagella and a crystalline surface layer was isolated from a mixed culture that completely reduces chlorinated ethenes to ethene. The organism coupled reductive dehalogenation of tetrachloroethene or trichloroethene to cis-1,2-dichloroethene to growth, using molecular hydrogen as the electron donor. It was unable to grow fermentatively or in the presence of tri- or tetrachloroethene with glucose, pyruvate, lactate, acetate or formate. The 16S rDNA sequence of strain TEA was 99.7% identical to that of Dehalobacter restrictus. The two organisms thus are representatives of the same species or the same genus within the Bacillus/Clostridium subphylum of the gram-positive bacteria.
Wild
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Institute of Microbiology, Swiss Federal Institute of Technology, ETH-Zentrum, Zürich, Switzerland.
Hermann
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R
Leisinger
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D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002712:Chlorides / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D004000:Dichloroethylenes / Q000378:metabolism; D004579:Electron Transport; D019143:Evolution, Molecular; D005407:Flagella / Q000648:ultrastructure; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013750:Tetrachloroethylene / Q000378:metabolism*; D014241:Trichloroethylene / Q000378:metabolism*
D002712:Chlorides; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004000:Dichloroethylenes; D014241:Trichloroethylene; D006859:Hydrogen; D010088:Oxidoreductases; D013750:Tetrachloroethylene; C035384:1,2-dichloroethylene