title: Plasminogen receptors. Turning Salmonella and Escherichia coli into proteolytic organisms.
abstract: We evaluated in vitro the hypothesis that bacterial adhesiveness to the mammalian extracellular matrix and the activation of plasminogen on bacterial plasminogen receptors promote bacterial penetration through basement membranes. We used the strain SH401 of Salmonella enterica serovar Typhimurium, which adheres to the high-mannose chains of laminin, a major glycoprotein of basement membranes, and expresses plasminogen receptors. Bacterium-bound plasmin was able to degrade laminin and extracellular matrix preparations as well as to potentiate the penetration of bacteria through a reconstituted basement membrane. The results suggest that metastatic tumour cells and bacterial pathogens use similar mechanisms to penetrate through tissue barriers.
authors.affiliation: Department of Biosciences, University of Helsinki, Finland.
authors.forename: T K
authors.initials: TK
authors.lastname: Korhonen
authors.forename: K
authors.initials: K
authors.lastname: Lähteenmäki
authors.forename: M
authors.initials: M
authors.lastname: Kukkonen
authors.forename: R
authors.initials: R
authors.lastname: Pouttu
authors.forename: U
authors.initials: U
authors.lastname: Hynönen
authors.forename: K
authors.initials: K
authors.lastname: Savolainen
authors.forename: B
authors.initials: B
authors.lastname: Westerlund-Wikström
authors.forename: R
authors.initials: R
authors.lastname: Virkola
mesh_terms: D001422:Bacterial Adhesion; D003094:Collagen; D004338:Drug Combinations; D004926:Escherichia coli / Q000472:pathogenicity; D005109:Extracellular Matrix / Q000382:microbiology; D005341:Fibrinolysin / Q000378:metabolism*; D006801:Humans; D007797:Laminin; D009361:Neoplasm Invasiveness; D010958:Plasminogen / Q000378:metabolism*; D011485:Protein Binding; D011509:Proteoglycans; D012486:Salmonella typhimurium / Q000472:pathogenicity*
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D010958:Plasminogen; D003094:Collagen; D005341:Fibrinolysin