Presence of multiple functional polyadenylation signals in the 3'-untranslated region of human corticotropin receptor cDNA.
We present 2.59 kb of the 3'-non-coding region of the ACTH receptor cDNA that contains seven potential polyadenylation signals. Among these signals, five are functional as detected by 3'-RACE and are consistent with the transcripts of 1.8, 3.4 and 4 kb visualized on Northern blots. We propose that the most likely molecular mechanism for the multiple ACTH-R mRNA transcripts is the alternative use of polyadenylation signals.
Penhoat
A
A
INSERM-INRA U 418, Hôpital Debrousse, Lyon, France. pdurand@univ-lyon1.fr
Naville
D
D
Jaillard
C
C
Durand
P
P
Bégeot
M
M
D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D018043:Receptors, Corticotropin / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D018043:Receptors, Corticotropin; D011061:Poly A