title: A closed system for the filtration of Mycobacterium bovis liquid cultures using disposable capsule filters.
abstract: A filtration system was designed to sterilize large volumes of Mycobacterium bovis BCG Tokyo culture safely, needed to purify protein antigens for immunodiagnosis of bovine tuberculosis. A closed system consists of culture bottles connected to three disposable filter capsules of decreasing pore size in series: a depth prefilter over a 1.2 microns filter; 0.8 micron prefilter over a 0.45 micron filter; and a 0.2 micron sterile filter. Low air pressure (3 psi) forces liquid from below the bacillary pellicle. The system features a stainless steel clamp to hold rubber stoppers on the culture bottles, pleated filters to exclude bacillary clumps, a quick disconnector to minimize aerosols, and a closed system with plastic disposable filters that can be autoclaved as a unit without dismantling.
authors.affiliation: Agriculture and Agri-Food Canada, Animal Diseases Research Institute, Nepean, Ontario, Canada.
authors.forename: E A
authors.initials: EA
authors.lastname: Sugden
authors.forename: P
authors.initials: P
authors.lastname: Martineau
authors.forename: E B
authors.initials: EB
authors.lastname: Rohonczy
authors.forename: K
authors.initials: K
authors.lastname: Stilwell
mesh_terms: D000336:Aerosols; D000391:Air Microbiology; D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000295:instrumentation*; D002417:Cattle; D005374:Filtration / Q000295:instrumentation*; D007159:Immunologic Tests; D009163:Mycobacterium bovis / Q000276:immunology / Q000302:isolation & purification*; D013242:Sterilization / Q000295:instrumentation; D014380:Tuberculosis, Bovine / Q000175:diagnosis
chemical_list: D000336:Aerosols; D000942:Antigens, Bacterial; D001426:Bacterial Proteins