Apolipoprotein secretion and lipid synthesis: regulation by fatty acids in newborn swine intestinal epithelial cells.
The IPEC-1 newborn swine intestinal epithelial cell line was used to determine the effects of the uptake of various fatty acids on the secretion of apolipoprotein (apo) B and apo A-I, as well as triglyceride and phospholipid. Long-chain saturated fatty acids were taken up and stimulated triglyceride synthesis, and palmitic (16:0) and stearic (18:0) acids also stimulated phospholipid synthesis. However, these fatty acids did not enhance triglyceride, phospholipid, or apo B or apo A-I secretion. Oleic acid (18:1) was the most effective of all fatty acids tested in stimulating triglyceride synthesis and the secretion of triglyceride, phospholipid, and apo B. Linoleic (18:2) and linolenic (18:3) acids were no more effective than long-chain saturated fatty acids in stimulating these processes. With saturated fatty acids, apo A-I followed the same secretory pattern as apo B. However, among the unsaturated fatty acids, oleic acid was the least effective and linolenic acid was the most effective in stimulating apo A-I secretion. Basolateral secretion of lipid and apolipoproteins by differentiated IPEC-1 cells is differentially regulated by apical exposure to fatty acids.
Wang
H
H
Department of Pediatrics, Arkansas Children's Hospital Research Institute, University of Arkansas for Medical Sciences, Little Rock 72202, USA.
Berschneider
H M
HM
Du
J
J
Black
D D
DD
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D016632:Apolipoprotein A-I / Q000378:metabolism; D001053:Apolipoproteins / Q000378:metabolism*; D001055:Apolipoproteins B / Q000378:metabolism; D002460:Cell Line; D005227:Fatty Acids / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D019301:Oleic Acid / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D013229:Stearic Acids / Q000494:pharmacology; D013552:Swine; D014280:Triglycerides / Q000378:metabolism
D016632:Apolipoprotein A-I; D001053:Apolipoproteins; D001055:Apolipoproteins B; D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D013229:Stearic Acids; D014280:Triglycerides; D019301:Oleic Acid; C031183:stearic acid; D007770:L-Lactate Dehydrogenase; D005990:Glycerol