title: Effects of manganese salts on the AIDS-related pathogen, Cryptosporidium parvum in vitro and in vivo.
abstract: The authors examined the effects of manganese salts on the interaction of the AIDS-related pathogen, Cryptosporidium parvum, with human ileoadenocarcinoma (HCT-8) cells in vitro. Manganese (Mn) inhibited binding of C. parvum sporozoite membrane antigens to intact, fixed HCT-8 cells in a dose-dependent fashion, whereas Ca++, Mg++, and Zn++ salts had no effect. Manganese was also found to affect sporozoite penetration of live HCT-8 cells, which resulted in a dose-dependent inhibition of parasite development. However, the levels of Mn++ needed in the live cell assays was approx 10-fold greater than in the fixed-cell assays. This inhibition of parasite development was not reversible when Ca++ or Mg++ were used as competitors. Oral supplementation of suckling mice infected with C. parvum with MnSO4 resulted in significant reductions and, in some cases, elimination of intestinally derived oocysts.
authors.affiliation: Division of Biology, Kansas State University, Manhattan 66506, USA. coccidia/ksu.edu
authors.forename: M V
authors.initials: MV
authors.lastname: Nesterenko
authors.forename: K M
authors.initials: KM
authors.lastname: Woods
authors.forename: S J
authors.initials: SJ
authors.lastname: Upton
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000469:parasitology; D000284:Administration, Oral; D000818:Animals; D000833:Animals, Suckling; D000953:Antigens, Protozoan / Q000187:drug effects / Q000378:metabolism; D000954:Antigens, Surface / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D003457:Cryptosporidiosis / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D006801:Humans; D015636:Magnesium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology*; D017895:Manganese Compounds / Q000008:administration & dosage / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D013431:Sulfates / Q000008:administration & dosage / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D017895:Manganese Compounds; D013431:Sulfates; D015636:Magnesium Chloride; D008345:Manganese; C039798:manganese sulfate