title: Intraspecific variation in Cryptocaryon irritans.
abstract: Intraspecific variation in the ciliate Cryptocaryon irritans was examined using sequences of the first internal transcribed spacer region (ITS-1) of ribosomal DNA (rDNA) combined with developmental and morphological characters. Amplified rDNA sequences consisting of 151 bases of the flanking 18 S and 5.8 S regions, and the entire ITS-1 region (169 or 170 bases), were determined and compared for 16 isolates of C. irritans from Australia, Israel and the USA. There was one variable base between isolates in the 18 S region and 11 variable bases in the ITS-1 region. Despite their similar morphology, significant sequence variation (4.1% divergence) and developmental differences indicate that Australian C. irritans isolates from estuarine (Moreton Bay) and coral reef (Heron Island) environments are distinct. The Heron Island isolate was genetically closer to morphologically dissimilar isolates from Israel (1.8% divergence) and the USA (2.3% divergence) than it was to the Moreton Bay isolates. Three isolates maintained in our laboratory since February 1994 differed in sequence from earlier laboratory isolates (2.9% to 3.5% divergence), even though all were similar morphologically and originated from the same source. During this time the sequence of the isolates from wild fish in Moreton Bay remained unchanged. These genetic differences indicate the existence of a founder effect in laboratory populations of C. irritans. The genetic variation found here, combined with known morphological and developmental differences, is used to characterise four strains of C. irritans.
authors.affiliation: Department of Parasitology, University of Queensland, Brisbane, Australia. diggles.ben@pi.sa.gov.au
authors.forename: B K
authors.initials: BK
authors.lastname: Diggles
authors.forename: R D
authors.initials: RD
authors.lastname: Adlard
mesh_terms: D000818:Animals; D016798:Ciliophora / Q000145:classification / Q000235:genetics*; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D005399:Fishes / Q000469:parasitology; D014644:Genetic Variation
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal