title: [A study on antiprothrombin antibodies in antiphospholipid syndrome].
abstract: In this study we investigated the frequency and the type of manifestations of antiprothrombin antibodies (aFII) in patients with antiphospholipid syndrome (APS). In 16 (84.2%) of 19 patients with lupus anticoagulant (LA) and either anticardiolipin antibodies or antiphosphatidylserine antibodies, two types of abnormal patterns were shown on a crossed immuno-electrophoresis technique using anti-human prothrombin murine IgG. The slow-moving peak of prothrombin was detected in 8 patients, while a peak in the other patients had the slow-moving shoulder. These slow-moving materials might represent prothrombin-aFII complexes. In 13 patients who were studied on Western blots, IgGs of 11 patients (84. 6%) bound to human purified prothrombin, and the IgGs of 7 (53.8%) of these patients also bound to human purified alpha-thrombin. Our results indicate that aFII detected in patients with APS may explain part of the mechanism of LA.
authors.affiliation: Department of Clinical Immunology and Hematology, Sapporo City General Hospital, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ieko
authors.forename: D A
authors.initials: DA
authors.lastname: Triplett
authors.forename: M
authors.initials: M
authors.lastname: Kohno
authors.forename: A
authors.initials: A
authors.lastname: Ohmoto
authors.forename: A
authors.initials: A
authors.lastname: Notoya
authors.forename: K
authors.initials: K
authors.lastname: Sawada
authors.forename: T
authors.initials: T
authors.lastname: Koike
mesh_terms: D000293:Adolescent; D000328:Adult; D016736:Antiphospholipid Syndrome / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011516:Prothrombin / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D011516:Prothrombin