title: Organization and expression of bovine TSPY.
abstract: We have isolated genomic sequences as well as transcripts from the bovine homolog of the human testis-specific protein, Y-encoded, TSPY which-in both species-is located on the Y Chromosome (Chr), organized as a gene family with a variable number of members, and expressed exclusively in the testis. 1266 bp of bovine TSPY specific sequence have been isolated from a testis cDNA library, by RT-PCR analyses and by Rapid Amplification of cDNA Ends (RACE). A bovine TSPY gene 4 is organized in seven exons, and transcripts are polyadenylated at various 3' ends. Consensus polyadenylation signals AAUUAAA are missing. Microheterogeneous sequence variation is found between TSPY family members. In addition, homologies to other Y-located repeated sequence families, BRY, have been discovered; these sequences are presumably derived from ancient members of the TSPY cluster, now forming a separate, probably nonfunctional subfamily. Bovine TSPY is subject to differential splicing. In the adult, it is expressed in early germ-cell stages, and expression could also be detected in fetal testis. Comparison with the human homolog shows the highest degree of similarity in the coding regions of exons 2, 3, and 4, which are also precisely conserved regarding their length.
authors.affiliation: Institut für Humangenetik, Medizinische Hochschule Hannover, D-30623 Hannover, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Vogel
authors.forename: F
authors.initials: F
authors.lastname: Dechend
authors.forename: E
authors.initials: E
authors.lastname: Manz
authors.forename: C
authors.initials: C
authors.lastname: Jung
authors.forename: S
authors.initials: S
authors.lastname: Jakubiczka
authors.forename: S
authors.initials: S
authors.lastname: Fehr
authors.forename: J
authors.initials: J
authors.lastname: Schmidtke
authors.forename: F
authors.initials: F
authors.lastname: Schnieders
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle / Q000235:genetics*; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009928:Organ Specificity; D011061:Poly A; D012326:RNA Splicing; D017386:Sequence Homology, Amino Acid; D051876:Sex-Determining Region Y Protein; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D014018:Tissue Distribution; D014157:Transcription Factors; D014158:Transcription, Genetic
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; C497749:TSPY1 protein, human; D014157:Transcription Factors; D011061:Poly A