Molecular cloning and characterization of a mono(ADP-ribosyl)transferase from human testis.
A human homologue of the rodent T cell mono(ADP-ribosyl)transferase RT6 mRNA was identified by a systematic partial sequencing of human testis transcripts. This messenger encodes for a precursor protein of 367 aa (MW: 41.5 kDa) which exhibits a peptide signal, consensus domains for mono(ADP-ribosyl)transferase and a C terminal part which contains three repeated motives (GEKNQKLEDH) and a region characteristic of glycophosphatidyl inositol anchored proteins. This mRNA is transcribed from a gene localized in 4q13-q21. Surprisingly, it is not expressed in human white blood cells but it exhibits a very specific testis expression in which it is likely to correspond to a new ADP-ribosyl transferase.
Lévy
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I
Unité INSERM 99, Hôpital Henri Mondor, Créteil, France.
Pawlak
A
A
Mattéi
M G
MG
Guellaën
G
G
D036002:ADP Ribose Transferases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D018076:DNA, Complementary; D058851:GPI-Linked Proteins; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger; D011817:Rabbits; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology*
D018076:DNA, Complementary; D058851:GPI-Linked Proteins; D011506:Proteins; D012333:RNA, Messenger; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; C449301:ART1 protein, human