Construction of a 5-Mb YAC contig from the putative 10q25 tumor-suppressor region for glioblastomas.
During the final step of the malignant progression to glioblastoma multiforme (GBM), the most frequent and malignant of primary brain tumors, more than 90% of the cases exhibit loss of genetic material on chromosome 10. We previously identified a 4-cM deletion interval in the 10q24-qter region that is common to all the GBM we have examined. A contig of 20 YACs spanning the 5 Mb of chromosomal DNA in the region has been assembled. Overlaps between YACs have been verified by STS content, fingerprinting analysis, and/or Alu-Alu PCR. The contig contains 17 known microsatellite markers, 15 new STSs derived from the insert ends of YACs, 9 ESTs, and 11 others STSs, for a total of 52 STSs (average marker density 1/100 kb). The physical map of this region will facilitate the search for a candidate tumor-suppressor gene(s) that is inactivated during the information of GBM.
Albarosa
R
R
Divisione di Biochimica e Genetica, Istituto Nazionale Neurologico C. Besta, Milan, Italy.
Finocchiaro
G
G
Chiariello
E
E
Russo
G
G
Susani
L
L
Vezzoni
P
P
Zucchi
I
I
D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016324:Sequence Tagged Sites
D017931:DNA Primers; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D005819:Genetic Markers