Mycobacterium tuberculosis Des protein: an immunodominant target for the humoral response of tuberculous patients.
The phoA gene fusion methodology permitted the identification of a new Mycobacterium tuberculosis exported protein, Des. This protein has significant sequence similarities to plant acyl-acyl carrier protein desaturases, which are enzymes involved in general fatty acid biosynthesis as well as in mycolic acid biosynthesis in mycobacteria. As shown by Western blot and enzyme-linked immunosorbent assay experiments, the Des protein is a major B-cell antigen that was recognized by all the tuberculous M. tuberculosis- and M. bovis-infected human patients tested.
Jackson
M
M
Unité de Génétique Mycobactérienne, Institut Pasteur, Paris, France.mjackson@pasteur.fr
Portnoï
D
D
Catheline
D
D
Dumail
L
L
Rauzier
J
J
Legrand
P
P
Gicquel
B
B
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial; D001402:B-Lymphocytes / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D002417:Cattle; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C106712:desA1 protein, Mycobacterium tuberculosis