VIDAS enzyme-linked fluorescent immunoassay for detection of Salmonella in foods: collaborative study.
The VIDAS SLM method for detection of Salmonella was compared with the Bacteriological Analytical Manual (BAM)/AOAC culture method in a collaborative study. Twenty laboratories participated in the evaluation. Each laboratory tested one or more of 6 test products: milk chocolate, nonfat dry milk, dried whole egg, soy flour, ground black pepper, and ground raw turkey. No significant differences (P < 0.05) were observed between the 2 methods. The 2 methods were in agreement for 99% of 1544 samples analyzed. Of the 20 samples out of agreement, 8 were VIDAS SLM positive and BAM/AOAC negative, and 12 were VIDAS SLM negative and BAM/AOAC positive. The VIDAS SLM method for detection of Salmonella in foods has been adopted first action by AOAC INTERNATIONAL.
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Silliker Laboratories Group, Inc., Corporate Research Center, South Holland, IL 60473, USA.
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D000818:Animals; D001431:Bacteriological Techniques; D002099:Cacao / Q000382:microbiology; D004531:Eggs / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015200:Fluoroimmunoassay / Q000379:methods*; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology; D012475:Salmonella / Q000302:isolation & purification*; D013025:Glycine max / Q000382:microbiology; D014422:Turkeys / Q000382:microbiology