title: Affinity and response of Burkholderia pseudomallei and Burkholderia cepacia to insulin.
abstract: The cells of Burkholderia pseudomallei, B. cepacia and Pseudomonas aeruginosa grown on agar plates were stained with fluorescently-labeled insulin. The former two species were stained positively indicating insulin binding but P. aeruginosa was not. Insulin exposure reduced phospholipase C and acid phosphatase activities of B. pseudomallei but did not affect those enzymatic activities of B. cepacia in the employed experimental conditions. It is suggested that B. pseudomallei have insulin receptors which may be associated with a signal transfer system involving phospholipase and protein tyrosine phosphatase.
authors.affiliation: National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
authors.forename: K
authors.initials: K
authors.lastname: Kanai
authors.forename: E
authors.initials: E
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Kurata
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D019121:Burkholderia Infections / Q000150:complications / Q000201:enzymology* / Q000382:microbiology; D016956:Burkholderia cepacia / Q000201:enzymology* / Q000472:pathogenicity; D016957:Burkholderia pseudomallei / Q000201:enzymology* / Q000472:pathogenicity; D048909:Diabetes Complications; D003920:Diabetes Mellitus / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D007328:Insulin; D000135:Acid Phosphatase; D010738:Type C Phospholipases