Expression of interstitial collagenase (matrix metalloproteinase-1) in gastric cancers.
The expression of matrix metalloproteinase-1 (MMP-1) gene and the presence of MMP-1 protein in gastric cancer were examined by in situ hybridization and immunohistochemistry. Expression of the interstitial collagenase (MMP-1) gene was detected within the stroma of the neoplastic glands, and infiltration of eosinophils was observed to be associated with regions of MMP-1 gene expression. The degree of eosinophilic infiltration correlated with the level of MMP-1 mRNA expression. Immunostaining showed localization of MMP-1 protein in the stromal cells, and additionally in the neoplastic glands. These findings indicate that the stromal cells may play an important role in the expression of MMP-1, and suggest a pathophysiological role for MMP-1 in the invasion and metastasis of gastric cancer.
Sakurai
Y
Y
Department of Surgery, School of Medicine, Keio University, Shinjuku-ku, Tokyo.
Otani
Y
Y
Kameyama
K
K
Hosoda
Y
Y
Okazaki
I
I
Kubota
T
T
Kumai
K
K
Kitajima
M
M
D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D017364:Collagenases / Q000378:metabolism*; D004804:Eosinophils / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D017403:In Situ Hybridization; D020781:Matrix Metalloproteinase 1; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D017364:Collagenases; D020781:Matrix Metalloproteinase 1