[Ribonuclease improves the function of hippocampal slices in the postischemic period].
Population spike amplitude was measured in hippocampal slices under conditions of 20-min glucose and oxygen deficit ("in vitro ischemia") with or without ribonuclease A. In control slices the response was gradually decreased within 3 +/- 2 min after the onset of hypoxia/hypoglycemia. This process continued during 13 +/- 6 min of reperfusion. The reperfusion restored the amplitude up to 70 +/- 17% of its initial level within 1.5 h after the onset of this procedure. Addition of ribonuclease delayed the beginning of the response decrease (by 8 +/- 2 min) and increased the level of its restoration (up to 113 +/- 23%) after the reperfusion. It is suggested that ribonuclease prevents from the energy exhaustion by preserving the cellular energy stores.
Kudriashov
I E
IE
Kudriashova
I V
IV
Raevskiĭ
V V
VV
D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D046508:Culture Techniques; D004558:Electric Stimulation; D004594:Electrophysiology; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D010477:Perfusion / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012260:Ribonucleases / Q000494:pharmacology*
D012260:Ribonucleases