Induction of macrophage inflammatory protein MIP-1alpha mRNA on glial cells after focal cerebral ischemia in the rat.
The distribution and cell source of macrophage inflammatory protein-1alpha (MIP-1alpha) mRNA induced by transient and permanent middle cerebral artery occlusion (MCAO) were investigated by a double in situ hybridization technique. The distribution and time course of the induction of MIP-1alpha mRNA were similar in the two MCAO models. MIP-1alpha mRNA was not detected in the sham-operated rat brain. MIP-1alpha mRNA was induced by MCAO with the peak of expression at 4-6 h after the onset of occlusion, and the signals of MIP-1alpha mRNA were observed in the ischemic core region at an earlier time point, and thereafter intensely in the penumbra of the ischemic area. The signals of MIP-1alpha mRNA were evident on Mac-1alpha mRNA-positive cells, but not on glial fibrillary acidic protein (GFAP) mRNA-positive cells, indicating that MIP-1alpha mRNA was induced in microglia/macrophages of the rat brain after focal cerebral ischemia.
Takami
S
S
Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Nishikawa
H
H
Minami
M
M
Nishiyori
A
A
Sato
M
M
Akaike
A
A
Satoh
M
M
D000818:Animals; D001157:Arterial Occlusive Diseases / Q000503:physiopathology; D001253:Astrocytes / Q000502:physiology*; D001345:Autoradiography; D002545:Brain Ischemia / Q000503:physiopathology*; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018076:DNA, Complementary; D015870:Gene Expression / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D017403:In Situ Hybridization; D019402:Macrophage Inflammatory Proteins / Q000235:genetics*; D008297:Male; D017628:Microglia / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018076:DNA, Complementary; D005904:Glial Fibrillary Acidic Protein; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger