A gene homologous to the Streptomyces chymotrypsin-like protease (SAM-p20) gene is tandemly located.
A gene encoding a homolog of the Streptomyces chymotrypsin-like serine protease, SAM-P20, was identified downstream of the sam-p20 gene and designated SAM-P20D. This gene has two tandem Shine Dalgarno sequences and two initiation codons. We have established vector systems with the function of tyrosinase gene-bone melanin pigmentation as a reporter for sam-p20D gene expression in Streptomyces coelicolor in order to identify the promoter and terminator activities. Using this system, the sam-p20D gene was suggested to be transcribed monocistronically.
Taguchi
S
S
Department of Biological Science and Technology, Science University of Tokyo, Japan. staguchi@rs.noda.sut.ac.jp
Ogawa
T
T
Endo
T
T
Momose
H
H
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics*; D013302:Streptomyces / Q000201:enzymology*; D013728:Terminator Regions, Genetic
D001426:Bacterial Proteins; C116416:SAM-P26 serine protease, Streptomyces; D012697:Serine Endopeptidases