Detection of lactate dehydrogenase-elevating virus in transplantable mouse tumors by biological assay and RT-PCR assays and its removal from the tumor cell.
It is known that lactate dehydrogenase-elevating virus (LDV) of mice is a common contaminant of transplantable tumors of both murine and human origin. It is imperative that tumors that are maintained by transplantation in mice are examined for LDV and freed of the virus, when present, before use in experimental studies, because an LDV infection of mice exerts considerable effects on lymphoid cell populations and cytokine production and other effects. Methods for LDV detection are described using a biological assay and reverse transcription (RT)-polymerase chain reaction (PCR) technology and their application is illustrated. A differential RT-PCR method that distinguishes between three quasispecies of LDV is also described and applied to an examination of LDVs isolated from a number of different tumors. Each of the LDV isolates was found to contain at least two different quasispecies, generally in different concentrations.
Chen
Z
Z
Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455, USA.
Plagemann
P G
PG
D000818:Animals; D018174:Arterivirus Infections / Q000097:blood / Q000235:genetics / Q000821:virology*; D001483:Base Sequence; D001681:Biological Assay / Q000379:methods*; D016679:Genome, Viral; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007772:Lactate dehydrogenase-elevating virus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation / Q000379:methods*; D009374:Neoplasms, Experimental / Q000737:chemistry / Q000821:virology*; D016133:Polymerase Chain Reaction
D007770:L-Lactate Dehydrogenase