title: Immunodetection of a protein related to mammalian arrestin in Euglena gracilis.
abstract: Six monoclonal antibodies directed against different epitopes of bovine retinal arrestin recognized a single polypeptide in extracts of achlorophyllous Euglena gracilis cells. This polypeptide had an apparent molecular weight slightly lower: 45kDa vs. 48 kDa, and a more basic isoelectric point compared to that of bovine visual arrestin. It was located in an insoluble cytoplasmic fraction. Immunofluorescence assays show a cytoplasmic punctuated pattern suggesting a cluster distribution or a linkage to some cell structure. The presence of arrestine-like molecules in achlorophyllous Euglena gracilis cells suggests that these proteins might be involved in a peculiar step of chemical signal transduction processes.
authors.affiliation: Laboratoire Métabolisme Cellulaire et Xénobiotiques, Faculté de Pharmacie, Chátenay-Malabry, France.
authors.forename: A
authors.initials: A
authors.lastname: Razaghi
authors.forename: J
authors.initials: J
authors.lastname: Bonaly
authors.forename: H
authors.initials: H
authors.lastname: Chacun
authors.forename: J P
authors.initials: JP
authors.lastname: Faure
authors.forename: M
authors.initials: M
authors.lastname: Mirshahi
authors.forename: J P
authors.initials: JP
authors.lastname: Barque
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D019393:Arrestin / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D017124:Conserved Sequence; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry / Q000235:genetics; D005056:Euglena gracilis / Q000276:immunology*; D019143:Evolution, Molecular; D015151:Immunoblotting; D007526:Isoelectric Point; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000737:chemistry / Q000276:immunology*; D012160:Retina / Q000737:chemistry / Q000276:immunology; D015398:Signal Transduction
chemical_list: D000911:Antibodies, Monoclonal; D019393:Arrestin; D000939:Epitopes; D015800:Protozoan Proteins