title: Chloroplast endoribonuclease p54 involved in RNA 3'-end processing is regulated by phosphorylation and redox state.
abstract: Chloroplast RNA-binding protein p54 is an endoribonuclease required for 3'end-processing of plastid precursor transcripts. We find that purified p54 can serve as a phosphate acceptor for protein kinases in vitro. Both the processing and RNA-binding activities of p54 are enhanced by phosphorylation and decreased by dephosphorylation. In addition, the enzyme is activated by the oxidized form of glutathione and inhibited by the reduced form, whereas other redox reagents that were tested showed no effect. Kinase treatment of p54 prior to oxidation by glutathione resulted in highest levels of activation, suggesting that phosphorylation and redox state act together to control p54 activity in vitro and possibly also in vivo.
authors.affiliation: Plant Cell Physiology and Molecular Biology, University of Bochum, D-44780 Bochum, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Liere
authors.forename: G
authors.initials: G
authors.lastname: Link
mesh_terms: D060365:Chloroplast Proteins; D002736:Chloroplasts / Q000201:enzymology*; D004722:Endoribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D009149:Mustard Plant; D010084:Oxidation-Reduction; D010766:Phosphorylation; D010946:Plants, Medicinal; D018087:Plastids; D011499:Protein Processing, Post-Translational; D012323:RNA Processing, Post-Transcriptional; D018749:RNA, Plant / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D014158:Transcription, Genetic
chemical_list: D060365:Chloroplast Proteins; D018749:RNA, Plant; D016601:RNA-Binding Proteins; D004722:Endoribonucleases; C106848:chloroplast RNA-binding protein p54