Protooncogene expression in osteogenesis induced by bone morphogenetic protein.
In this study, changes in the expression of protooncogenes c-fos and c-myc messenger ribonucleic acid were investigated in mice after implantation of bone morphogenetic protein. The expression of c-fos showed a biphasic pattern. The first increase was observed on Day 1 with the aggregation of round cells. The second increase was observed on Day 7 with the appearance of chondroblasts. The amount of c-myc messenger ribonucleic acid showed the sustained high levels from Days 2 to 7. During this period, the proliferation of mesenchymal cells was histologically evident. After Day 11, the expression of c-fos and c-myc decreased and remained at low levels despite the progress in chondroosteogenesis. The protooncogenes c-fos and c-myc appear to increase before calcification in the process of bone morphogenetic protein induced bone and cartilage development.
Sakano
S
S
Department of Orthopaedic Surgery, Nagoya University School of Medicine, Japan.
Murata
Y
Y
Iwata
H
H
Sato
K
K
Ito
T
T
Kurokouchi
K
K
Seo
H
H
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology* / Q000502:physiology; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos; D016259:Genes, myc; D051379:Mice; D008809:Mice, Inbred C3H; D018482:Muscle, Skeletal / Q000473:pathology; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits
D019485:Bone Morphogenetic Proteins; D012333:RNA, Messenger