title: Aberrant O-glycosylation in the collagenous domain of pro alpha2(I) procollagen subunits synthesized by chemically transformed hamster fibroblasts.
abstract: Chemically transformed Syrian hamster embryo fibroblasts (NQT-SHE) do not synthesize the pro alpha1(I) subunit of type I collagen, but they secrete two forms of the pro alpha2(I) subunit (N33 and N50) with abnormal post-translational modifications localized in the alpha2CB3,5 cyanogen bromide peptide of the collagenous domain (B. Peterkofsky and W. Prather (1992) J. Biol. Chem. 267 5388-5395). Isoelectric focusing and treatment of the modified chains with glycosidases and biotinylated Jacalin lectin identified the modifications as Gal beta1,3-GalNAc-O-Ser/Thr with or without a terminal sialic acid in an alpha2,6 linkage. Unhydroxylated N33 alpha-chains also reacted with Jacalin, confirming that the abnormal modification was O-glycosylation and not hyperhydroxylation of proline or lysine. Cells were treated with benzyl GalNAc, a competitive inhibitor of galactosyl transferase that prevents addition of Gal to GalNAc-O-Ser/Thr and thus blocks elongation of O-glycosyl chains. Treated cells secreted pro alpha2(I) chains containing GalNAc-O-Ser/Thr but no galactose or sialic acid, which suggested that Gal addition takes place before sialylation. Treatment of NQT-SHE cells with monensin and brefeldin A inhibited secretion and led to intracellular accumulation of pro alpha2(I) chains that contained only GalNAc. Therefore, it appears that GalNAc addition to pro alpha2(I) chains in NQT-SHE cells occurs in the cis-Golgi, while sialic acid and galactose are added in the trans-Golgi network. The pro alpha2(I) chains produced by NQT-SHE cells most likely are modified because they are in the denatured state, and thus potential O-glycosylation sites become available that would not be exposed in normal triple helical procollagen.
authors.affiliation: Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892-4255, USA.
authors.forename: U
authors.initials: U
authors.lastname: Ramachandran
authors.forename: B
authors.initials: B
authors.lastname: Peterkofsky
mesh_terms: D000116:Acetylgalactosamine / Q000031:analogs & derivatives; D000818:Animals; D001593:Benzyl Compounds; D001665:Binding Sites; D020126:Brefeldin A; D002461:Cell Line, Transformed; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D006224:Cricetinae; D003517:Cyclopentanes / Q000494:pharmacology; D004791:Enzyme Inhibitors; D006026:Glycoside Hydrolases; D006031:Glycosylation; D007525:Isoelectric Focusing; D008985:Monensin / Q000494:pharmacology; D011347:Procollagen / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001593:Benzyl Compounds; D003517:Cyclopentanes; D004791:Enzyme Inhibitors; D011347:Procollagen; D020126:Brefeldin A; C080739:benzyl-alpha-N-acetylgalactosamine; D003094:Collagen; D008985:Monensin; D006026:Glycoside Hydrolases; D000116:Acetylgalactosamine