title: 32P-Postlabeling high-performance liquid chromatographic improvements to characterize DNA adduct stereoisomers from benzo[a]pyrene and benzo[c]phenanthrene, and to separate DNA adducts from 7,12-dimethylbenz[a]anthracene.
abstract: Single compounds can generate complex DNA adduct patterns by reactions through different pathways, with different target nucleotides and through different configurations of the products. DNA adduct analysis by 32P-HPLC was improved by adding an isocratic plateau in an otherwise linear gradient, thereby enhancing resolution of predictable retention time intervals. This enhanced 32P-HPLC technique was used to analyze and at least partly resolve 14 out of 16 available benzo[c]phenanthrene deoxyadenosine and deoxyguanosine adduct standards, 8 out of 8 available benzo[a]pyrene deoxyadenosine and deoxyguanosine adduct standards, and 51 peaks from 7,12-dimethylbenz[a]anthracene-calf thymus DNA reaction products. The same type of gradient modifications could be used to enhance resolution in analyses of other complex DNA adduct mixtures, e.g., in vivo in humans.
authors.affiliation: Karolinska Institutet, Department of Bioscience, NOVUM Research Park, Huddinge, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Zeisig
authors.forename: L
authors.initials: L
authors.lastname: Möller
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis*; D002273:Carcinogens; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000032:analysis*; D003839:Deoxyadenosines; D003849:Deoxyguanosine; D009153:Mutagens; D010616:Phenanthrenes; D010761:Phosphorus Radioisotopes; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: C059444:7,12-dimethylbenz(a)anthracene-DNA adduct; D002273:Carcinogens; D018736:DNA Adducts; D003839:Deoxyadenosines; D009153:Mutagens; D010616:Phenanthrenes; D010761:Phosphorus Radioisotopes; C056612:benzo(a)pyrene-DNA adduct; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D003849:Deoxyguanosine; C002615:benzo(c)phenanthrene