Effects of macrophage- and granulocyte-colony stimulating factors on thymidylate synthase and thymidine kinase activity in rat hematopoietic cells.
The effects of macrophage (M) and granulocyte (G) colony-stimulating factors (CSFs) on the activity of thymidylate synthase and thymidine kinase, which are involved in de novo and salvage pathways for pyrimidine nucleotide synthesis, were investigated in the hematopoietic cells of rats treated with cyclophasphamide. Thymidine kinase activity, but not that of thymidylate synthase, was markedly enhanced in these cells by M- and G-CSF treatment (p < 0.05 and p < 0.01). G-CSF directly, and M-CSF indirectly stimulate myeloid cells and lead to S-phase predominantly via the salvage pathway for pyrimidine nucleotide synthesis. The present study indicates that these CSFs can be effective inducers of complete remission in acute leukemias when employed together with chemotherapy.
Kudo
H
H
Division of Blood Transfusion, Internal Medicine, Tokyo Metropolitan Bokuto General Hospital, Japan.
Suzuki
S
S
Kuwa
K
K
Nakayama
T
T
Maemura
M
M
Sakamoto
S
S
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000201:enzymology*; D002478:Cells, Cultured; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006410:Hematopoiesis; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013937:Thymidine Kinase / Q000032:analysis*; D013940:Thymidylate Synthase / Q000032:analysis*
D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D013940:Thymidylate Synthase; D013937:Thymidine Kinase