Detection of toxic organometallic complexes in wastewaters using algal assays.
Chlorella (a unicellular green alga) and Cladophora (a filamentous alga) were used in algal assays to identify the presence and toxicity of organometallic complexes in four industrial wastewaters. Toxicities of inorganic Pb and organometallic compounds (trimethyl, tetramethyl and tetraethyl leads, cacodylic acid and Cu-picolinate) were examined, using algal cells grown in 10% BBM solution. Inorganic Pb and organometallic compounds altered the fine structure of Chlorella cells in a distinguishable manner. X-ray microanalysis revealed that organometallic compounds accumulated in the neutral lipids of Cladophora cells. By applying the above techniques to the wastewater assays, two of the four wastewaters tested were found to contain organometallic complexes. Wastewater from a chemical company contained only traces of organo-Cu, but one mining effluent contained significant quantities of organo-Cu and organo-Pb, and traces of organo-Cr and organo-Tl (thallium). These studies suggest that X-ray microanalysis of algae may be a useful tool in identifying aquatic systems contaminated with metals and organometallic compounds.
Wong
S L
SL
Ontario Ministry of Environment and Energy, Environmental Monitoring and Reporting Branch, Biomonitoring Section, 125 Resources Rd, Etobicoke, Ontario, M9P 3V6, Canada.
Nakamoto
L
L
Wainwright
J F
JF
D002708:Chlorella / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008670:Metals / Q000032:analysis / Q000633:toxicity; D015388:Organelles / Q000187:drug effects / Q000648:ultrastructure; D009942:Organometallic Compounds / Q000032:analysis* / Q000633:toxicity; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014877:Water Pollution, Chemical / Q000032:analysis*
D008670:Metals; D009942:Organometallic Compounds; D014874:Water Pollutants, Chemical; D014867:Water