title: The sequence of the 5' end of the U8 small nucleolar RNA is critical for 5.8S and 28S rRNA maturation.
abstract: Ribosome biogenesis in eucaryotes involves many small nucleolar ribonucleoprotein particles (snoRNP), a few of which are essential for processing pre-rRNA. Previously, U8 snoRNA was shown to play a critical role in pre-rRNA processing, being essential for accumulation of mature 28S and 5.8S rRNAs. Here, evidence which identifies a functional site of interaction on the U8 RNA is presented. RNAs with mutations, insertions, or deletions within the 5'-most 15 nucleotides of U8 do not function in pre-rRNA processing. In vivo competitions in Xenopus oocytes with 2'O-methyl oligoribonucleotides have confirmed this region as a functional site of a base-pairing interaction. Cross-species hybrid molecules of U8 RNA show that this region of the U8 snoRNP is necessary for processing of pre-rRNA but not sufficient to direct efficient cleavage of the pre-rRNA substrate; the structure or proteins comprising, or recruited by, the U8 snoRNP modulate the efficiency of cleavage. Intriguingly, these 15 nucleotides have the potential to base pair with the 5' end of 28S rRNA in a region where, in the mature ribosome, the 5' end of 28S interacts with the 3' end of 5.8S. The 28S-5.8S interaction is evolutionarily conserved and critical for pre-rRNA processing in Xenopus laevis. Taken together these data strongly suggest that the 5' end of U8 RNA has the potential to bind pre-rRNA and in so doing, may regulate or alter the pre-rRNA folding pathway. The rest of the U8 particle may then facilitate cleavage or recruitment of other factors which are essential for pre-rRNA processing.
authors.affiliation: Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-1766, USA. bp51h@nih.gov
authors.forename: B A
authors.initials: BA
authors.lastname: Peculis
mesh_terms: D000818:Animals; D001483:Base Sequence; D002466:Cell Nucleolus / Q000378:metabolism*; D005816:Genetic Complementation Test; D006801:Humans; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009694:Nucleic Acid Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012339:RNA, Ribosomal, 28S / Q000378:metabolism*; D012340:RNA, Ribosomal, 5.8S / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000235:genetics*; D012261:Ribonucleoproteins / Q000502:physiology*; D013045:Species Specificity; D013329:Structure-Activity Relationship; D014982:Xenopus laevis
chemical_list: D009694:Nucleic Acid Precursors; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins