Enhanced in vivo catalytic activity of PEG-modified cellulase complex from Trichoderma reesei.
The cellulase complex from Trichoderma reesei was polyethylene glycol (PEG)-modified with considerable retention of endo-beta-1,4-glucanase activity, as evaluated by the carboxymethylcellulase (CMCase) assay. While resistance towards heat denaturation was the same for either form, susceptibility towards proteolysis was slightly greater for the PEG-conjugate, in contrast to most reports using other enzymes. The circulatory life of endoglucanase activity associated with the complex was enhanced upon PEG-modification. This was confirmed by demonstrating degradation of chromogen-labelled substrate injected (i.v.) after 24hr of administration of the PEG-ylated enzymes; in contrast the substrate remained undegraded in mice pretreated with the native complex. The PEG-modified complex could be a good candidate for the treatment of pneumoconioses of textile workers exposed to cotton dust.
Mitra
G
G
Department of Biochemistry, University of Delhi, India. bic_dusc@ernet.in
Bachhawat
B K
BK
D000818:Animals; D015153:Blotting, Western; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002384:Catalysis; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism* / Q000493:pharmacokinetics; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011489:Protein Denaturation; D014242:Trichoderma / Q000201:enzymology*
D004396:Coloring Agents; D011092:Polyethylene Glycols; D006026:Glycoside Hydrolases; D002480:Cellulase; C019826:carboxymethylcellulase; D002266:Carboxymethylcellulose Sodium