title: A unique metabolite of nimesulide.
abstract: Nimesulide is a nonsteroidal anti-inflammatory drug recently detected in equine blood and urine samples taken at the race track. The detection of the drug in a blood sample led to the identification of an unknown thin-layer chromatographic (TLC) spot in track urine samples as a metabolite of nimesulide. Characterization of the unknown TLC spot and comparison with the synthesized compound shows that the unknown TLC spot is a previously unreported equine metabolite of nimesulide. The metabolite was identified as resulting from the reduction of the nitro group on nimesulide to an amino group. This reduced nitro metabolite (4-amino-2-phenoxy-methanesulfonanilide) is a major metabolite of nimesulide in the equine.
authors.affiliation: Novamann (Ontario) Inc., Mississauga, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Sarkar
authors.forename: J M
authors.initials: JM
authors.lastname: McIntosh
authors.forename: R
authors.initials: R
authors.lastname: Leavitt
authors.forename: H
authors.initials: H
authors.lastname: Gouthro
mesh_terms: D000284:Administration, Oral; D000813:Anilides / Q000302:isolation & purification / Q000652:urine*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine; D002855:Chromatography, Thin Layer / Q000662:veterinary; D004300:Doping in Sports; D006736:Horses / Q000378:metabolism* / Q000652:urine; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared / Q000662:veterinary; D013449:Sulfonamides / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine
chemical_list: C108971:4-amino-2-phenoxymethanesulfonanilide; D000813:Anilides; D000894:Anti-Inflammatory Agents, Non-Steroidal; D013449:Sulfonamides; C012655:nimesulide