Properties of Syrian hamster cells transformed by human papillomavirus type 16.
Adult Syrian hamster kidney cells were transfected with a mixture of plasmids containing human papillomavirus type 16 (HPV16) E6/E7 open reading frames (ORFs), activated Ha-ras gene and neomycin resistance gene. From these cultures two lines were isolated which were oncogenic for newborn and 5-day-old but not for 3-week-old hamsters. Sublines oncogenic for 3-7-week-old hamsters were derived from tumours formed in animals inoculated within 5 days of birth. The cells contained HPV 16 DNA in an integrated form and HPV16 transcripts. The transcript patterns in low and high oncogenicity sublines were different. Very few tumour-bearing animals possessed antibodies reactive with E6- and E7-derived synthetic peptides. On the other hand a majority of these animals gave a positive reaction in the lymphoproliferation assay with either E6 and E7 peptides or extracts from the transformed cells.
Kitasato
H
H
Department of Experimental Virology, Institute of Haematology and Blood Transfusion, Prague, Czech Republic.
Vonka
R
R
Bostík
P
P
Hamsíková
E
E
Sobotková
E
E
Smahel
M
M
Vonka
V
V
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D008647:Mesocricetus; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000276:immunology; D027383:Papillomaviridae / Q000235:genetics* / Q000276:immunology; D050725:Papillomavirus E7 Proteins; D012367:RNA, Viral / Q000032:analysis; D012097:Repressor Proteins; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000914:Antibodies, Viral; D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012367:RNA, Viral; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16