[Effect of ischemic damage factors on lipid peroxidation in rat brain synaptosomes].
Accumulation of TBA-reactive substances after a 40 min incubation of rat brain synaptosomes at 37 degrees C was analysed. A lowering of pH to 6.5-5.5 or arachidonic acid (0.1-1.0 mM) increased lipid peroxidation which was blocked by antioxidants. Acidosis (pH 6.0) and arachidonic acid used in combination had a strong synergic effect. Depolarisation of plasma membranes or intrasynaptosomal mitochondria were without influence on lipid peroxidation at neutral or acid pH. The results support a leading role of acidosis and phospholipases in stimulation of peroxidation under ischaemia.
Lyskova
T I
TI
Alsemtsev
S L
SL
Fedorovich
S V
SV
Levko
A V
AV
Rakovich
A A
AA
Samoĭlenko
S G
SG
Fedulov
A S
AS
Konev
S V
SV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D016718:Arachidonic Acid / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D015227:Lipid Peroxidation; D008928:Mitochondria / Q000378:metabolism; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D016718:Arachidonic Acid