Resonance Raman spectral properties and stability of manganese protoporphyrin IX cytochrome b5.
The structure and stability of cytochrome b5 reconstituted with manganese protoporphyrin IX instead of iron protoporphyrin IX has been investigated by resonance Raman spectroscopy and stopped-flow visible spectroscopy. The resonance Raman spectrum of MnIII cytochrome b5 was consistent with a high-spin hexacoordinate MnIII protoporphyrin IX structure that converted to a high-spin pentacoordinate structure at higher laser power. The resonance Raman spectrum of MnII cytochrome b5 indicated a high-spin pentacoordinate structure which was independent of laser power. Studies of the binding of MnIII protoporphyrin IX to apocytochrome b5 indicated that the MnIII-containing porphyrin bound much less tightly to the protein than did heme. Although the second-order rate constant at 20 degrees C for the association of heme with apocytochrome b5 (4.5 x 10(7) M(-1) s(-1)) was estimated to be only 1 order of magnitude higher than that with Mn protoporphyrin IX (3.3 x 10(6) M(-1) s(-1)), the dissociation of manganese substituted cytochrome b5 into the apoprotein and free Mn protoporphyrin IX occurs with a first-order rate constant of 1.2 x 10(-2) s(-1) at 20 degrees C while the dissociation of heme from cytochrome b5 at room temperature occurs 3 orders of magnitude more slowly with a first-order rate constant of 1.67 x 10(-5) s(-1) [Vergeres, G., Chen, D. Y., Wu, F.F., & Waskell, L. (1993) Arch. Biochem. Biophys. 305, 231-241]. The equilibrium dissociation constant for manganese-substituted cytochrome b5 increased with temperature from 4 nM at 20 degrees C to 14 nM at 37 degrees C. These results suggest that, in the reconstituted cytochrome P450 metabolizing system, especially in studies done with low protein concentrations (0.1 microM), and at elevated temperatures (37 degrees C), as much as 30% of the manganese-substituted cytochrome b5 may dissociate to free Mn-protoporphyrin IX and apocytochrome b5.
Gruenke
L D
LD
Department of Anesthesia and the Liver Center, University of California, and the Veterans Administration Medical Center, San Francisco 94121, USA.
Sun
J
J
Loehr
T M
TM
Waskell
L
L
D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D002417:Cattle; D003573:Cytochrome b Group / Q000378:metabolism; D045303:Cytochromes b; D015786:Cytochromes b5 / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D004795:Enzyme Stability; D006418:Heme / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D008345:Manganese / Q000378:metabolism*; D009994:Osmolar Concentration; D011524:Protoporphyrins / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D013696:Temperature
D001059:Apoproteins; D003573:Cytochrome b Group; D008024:Ligands; D011524:Protoporphyrins; C038654:manganese protoporphyrin; D006418:Heme; D008345:Manganese; D045303:Cytochromes b; D015786:Cytochromes b5