Developmental regulation of CRD-BP, an RNA-binding protein that stabilizes c-myc mRNA in vitro.
We previously isolated and characterized a coding region determinant-binding protein (CRD-BP) that might regulate c-myc mRNA post-transcriptionally. CRD-BP binds specifically to the coding region of c-myc mRNA and might stabilize c-myc mRNA in vitro by protecting it from endonucleolytic cleavage. Since c-myc abundance is regulated during embryonic development and cell replication, we investigated whether CRD-BP is also regulated in animal tissues. We focused on CRD-BP expression during rat liver development and liver regeneration, because c-myc mRNA is regulated post-transcriptionally in both cases. CRD-BP expression parallels c-myc expression during liver development; the protein is present in fetal and neonatal liver but is absent or in low abundance in adult liver. In contrast, the up-regulation of c-myc mRNA following partial hepatectomy is not accompanied by up-regulation of CRD-BP. To our knowledge, CRD-BP is the first example of a putative mammalian mRNA-binding protein that is abundant in a fetal tissue but either absent from or scarce in adult tissues. Its expression in fetal liver and in transformed cell lines suggests CRD-BP is an oncofetal protein.
Leeds
P
P
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706, USA.
Kren
B T
BT
Boylan
J M
JM
Betz
N A
NA
Steer
C J
CJ
Gruppuso
P A
PA
Ross
J
J
D000818:Animals; D018507:Gene Expression Regulation, Developmental; D016259:Genes, myc; D006498:Hepatectomy; D008099:Liver / Q000196:embryology / Q000254:growth & development / Q000502:physiology; D008115:Liver Regeneration / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D016601:RNA-Binding Proteins