title: Role of bacterial Mn-cofactored superoxide dismutase in oxidative stress responses, nasopharyngeal colonization, and sustained bacteremia caused by Haemophilus influenzae type b.
abstract: Haemophilus influenzae type b, a causative agent of bacterial sepsis and meningitis in young children, contains a single superoxide dismutase (SOD), a cytoplasmic MnSOD. To study the role of this enzyme, a chromosomal sodA::lacZ mutant (M-2) was constructed. M-2 had an increased sensitivity towards oxygen and the redox-active agent paraquat. A 3.4-fold increase in sodA-lacZ expression was found in M-2 grown with oxygen supply rates between 3 and 36 mmol of O2/liter/h. In similar experiments with the wild type, assaying SodA activity, a 3.1-fold increase was found. Both the wild type and M-2 grew best at the lowest oxygen supply rate tested, consistent with the notion that H. influenzae prefers a more anaerobic environment. In the infant rat model of infection, the ability of M-2 to colonize the nasopharynx was found to be impaired, but its ability to cause invasive disease was unaffected. This suggests that after invasion, the growth disadvantage imposed by a SodA- phenotype is not limiting.
authors.affiliation: Department of Paediatrics, Imperial College School of Medicine at St. Mary's Hospital, London, United Kingdom.
authors.forename: R A
authors.initials: RA
authors.lastname: D'Mello
authors.forename: P R
authors.initials: PR
authors.lastname: Langford
authors.forename: J S
authors.initials: JS
authors.lastname: Kroll
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000209:etiology*; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000254:growth & development* / Q000472:pathogenicity; D009154:Mutation; D009666:Nose / Q000382:microbiology*; D018384:Oxidative Stress; D010614:Pharynx / Q000382:microbiology*; D051381:Rats; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
chemical_list: D013482:Superoxide Dismutase