Conjugation-specific genes in the ciliate Euplotes crassus: gene expression from the old macronucleus.
Following mating or conjugation, the hypotrichous ciliate Euplotes crassus undergoes a massive genome reorganization process. While the nature of the rearrangement events has been well studied, little is known concerning proteins that carry out such processes. As a means of identifying such proteins, differential screening of a developmental cDNA library, as well as construction of a cDNA subtraction library, was used to isolate genes expressed only during sexual reproduction. Five different conjugation-specific genes have been identified that are maximally expressed early in conjugation, during the period of micronuclear meiosis, which is just prior to macronuclear development and the DNA rearrangement process. All five genes are retained in the mature macronucleus. Micronuclear, macronuclear, and cDNA clones of one gene (conZA7) have been sequenced, and the results indicate that the gene encodes a putative DNA binding protein. In addition, the presence of an internal eliminated sequence in the micronuclear copy of the conZA7 gene indicates that this conjugation-specific gene is transcribed from the old macronucleus.
Ling
Z
Z
Department of Biochemistry, University of Connecticut Health Center, Farmington 06030, USA.
Ghosh
S
S
Jacobs
M E
ME
Klobutcher
L A
LA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D018076:DNA, Complementary; D016054:DNA, Protozoan; D004268:DNA-Binding Proteins / Q000235:genetics; D016812:Euplotes / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015800:Protozoan Proteins / Q000235:genetics; D016053:RNA, Protozoan; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D016054:DNA, Protozoan; D004268:DNA-Binding Proteins; D015800:Protozoan Proteins; D016053:RNA, Protozoan; C105824:conZA7 protein, Euplotes crassus