title: Nuclear factor-kappaB activity and arterial response to balloon injury.
abstract: We studied the effect of arterial balloon injury on nuclear factor-kappaB (NF-kappaB) mobilization and ICAM-1 expression in untreated rats and rats treated with aspirin. Baseline NF-kappaB nuclear binding in smooth muscle cells (SMC) increased two-fold within 6 h after balloon injury. The binding returned to baseline 3 days after injury. Consistently nuclear staining of p65 active subunit increased in the medial SMC following balloon injury. There was no baseline ICAM-1 expression. Within 3 days after balloon injury there was marked medial ICAM-1 expression, that localized to neointima 7 days after injury and to regrowing endothelial cells 14 days after injury. Treatment with aspirin inhibited NF-kappaB nuclear translocation and binding and was associated with reduction of ICAM-1 expression, SMC proliferation and neointimal thickening following balloon injury. These data suggest that transient mobilization of NF-kappaB in vascular SMC after balloon injury mediates ICAM-1 expression and is involved in arterial response to balloon injury.
authors.affiliation: Atherosclerosis Research Center, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: B
authors.initials: B
authors.lastname: Cercek
authors.forename: M
authors.initials: M
authors.lastname: Yamashita
authors.forename: P
authors.initials: P
authors.lastname: Dimayuga
authors.forename: J
authors.initials: J
authors.lastname: Zhu
authors.forename: M C
authors.initials: MC
authors.lastname: Fishbein
authors.forename: S
authors.initials: S
authors.lastname: Kaul
authors.forename: P K
authors.initials: PK
authors.lastname: Shah
authors.forename: J
authors.initials: J
authors.lastname: Nilsson
authors.forename: J
authors.initials: J
authors.lastname: Regnstrom
mesh_terms: D000818:Animals; D001158:Arteries / Q000293:injuries*; D001241:Aspirin / Q000494:pharmacology; D002404:Catheterization / Q000009:adverse effects*; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000648:ultrastructure; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1; D001241:Aspirin