High-performance liquid chromatographic determination of acrolein as a marker for cyclophosphamide bioactivation in human liver microsomes.
A high-performance liquid chromatographic method for the quantification of acrolein following incubation of cyclophosphamide (CP) with human liver microsomes was developed. Based on the formation of the fluorescent derivative 7-hydroxyquinoline by condensation of acrolein with 3-aminophenol quantitation was performed without prior extraction or other sample cleanup procedures. The method showed sufficient sensitivity with a limit of detection of 5 ng/ml and a limit of quantification of 10 ng/ml. The suitability of the method is shown for enzyme kinetic studies.
Bohnenstengel
F
F
Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart, Germany.
Eichelbaum
M
M
Golbs
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E
Kroemer
H K
HK
D000171:Acrolein / Q000032:analysis* / Q000493:pharmacokinetics; D000627:Aminophenols; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000493:pharmacokinetics*; D015415:Biomarkers / Q000032:analysis; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003520:Cyclophosphamide / Q000493:pharmacokinetics*; D006801:Humans; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D012680:Sensitivity and Specificity
D000627:Aminophenols; D018906:Antineoplastic Agents, Alkylating; D015415:Biomarkers; D000171:Acrolein; D003520:Cyclophosphamide; C055528:3-aminophenol