title: Lower density of antral somatostatin-immunoreactive cells in the digestive form of chronic Chagas' disease.
abstract: Patients with the digestive form of chronic Chagas' disease exhibit abnormally increased gastrin release, possibly caused by antral gastrin cell (G cell) hyperfunction. In order to identify the mechanisms underlying this abnormality, we used an immunohistochemical method to assess the population of antral somatostatin-producing cells (D cells) in chagasic patients, since somatostatin is known to be the main inhibitory factor of gastrin secretion. Samples (N = 11) of endoscopic antral biopsies taken from 16 Chagas' disease patients and 13 control subjects were studied. Antral D and G cell populations were determined by an immunohistochemical technique using highly specific antibodies against somatostatin and gastrin. There was no significant difference between Chagas' disease and control groups regarding G cell population (number of cells/mm reported as median (range): 70.0 (23.7-247.0) vs 98.1 (52.7-169.4), P > 0.10). In contrast, the number of antral D cells in Chagas' disease patients was significantly lower than in controls (16.4 (6.9-54.4) vs 59.3 (29.6-113.8), P < 0.05). Chronic superficial gastritis and infection with Helicobacter pylori were more frequent in chagasic patients than in controls, but there was no demonstrable association between these factors and the reduction of the number of antral D cells. These data suggest that reduction in the number of antral somatostatin-producing cells, which should lead to reduced inhibition of gastrin cell activity, may play a role in the increased gastrin secretion observed in Chagas' disease patients.
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
authors.forename: L E
authors.initials: LE
authors.lastname: Troncon
authors.forename: A J
authors.initials: AJ
authors.lastname: Barbosa
authors.forename: J N
authors.initials: JN
authors.lastname: Carvalhais
authors.forename: L M
authors.initials: LM
authors.lastname: Romanello
mesh_terms: D014355:Chagas Disease / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D002908:Chronic Disease; D005753:Gastric Mucosa / Q000737:chemistry / Q000473:pathology*; D005755:Gastrins / Q000378:metabolism*; D016481:Helicobacter Infections / Q000150:complications; D016480:Helicobacter pylori; D006801:Humans; D011706:Pyloric Antrum / Q000737:chemistry / Q000473:pathology; D013004:Somatostatin / Q000032:analysis* / Q000276:immunology
chemical_list: D005755:Gastrins; D013004:Somatostatin