title: Birth from cryopreserved embryos following in-vitro maturation of oocytes and intracytoplasmic sperm injection.
abstract: This case report describes the birth of a baby following the transfer of cryopreserved embryos generated from intracytoplasmic sperm injection (ICSI) carried out on the second day after oocyte pick-up of in-vitro-matured metaphase I and germinal vesicle stage oocytes. The couple had a history of three failed intrauterine insemination attempts and reduced fertilization rates in two previous in-vitro fertilization (IVF) cycles. In the IVF-ICSI treatment cycle, 6/11 mature oocytes became fertilized following ICSI on the first day. However, the patient failed to conceive following the transfer of three embryos. Five oocytes were immature (two at metaphase I stage and three with a germinal vesicle) and these were cultured overnight. All had extruded a polar body by the following day and ICSI was therefore performed; four oocytes became fertilized, and were cryopreserved at the pronulear stage in propanediol. In the next treatment cycle, transfer of frozen embryos was planned. The pronuclear zygotes were thawed and cultured for 24 h prior to the transfer of two embryos in a cycle stimulated with low doses of follicle stimulating hormone. This resulted in a pregnancy and the delivery of a healthy baby boy. In-vitro maturation of metaphase I and germinal vesicle oocytes which are routinely collected in IVF-ICSI cycles, followed by second day ICSI fertilization, may provide a valuable source of embryos for infertile couples.
authors.affiliation: PIVET Medical Centre, Leederville, Perth, Western Australia.
authors.forename: W R
authors.initials: WR
authors.lastname: Edirisinghe
authors.forename: S M
authors.initials: SM
authors.lastname: Junk
authors.forename: P L
authors.initials: PL
authors.lastname: Matson
authors.forename: J L
authors.initials: JL
authors.lastname: Yovich
mesh_terms: D000328:Adult; D015925:Cryopreservation; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro; D005615:Freezing; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D013094:Spermatozoa