title: Changes in intracellular cytokine levels in lymphocytes induced by measles virus.
abstract: We investigated the changes in intracellular cytokine levels in different lymphocyte populations induced by measles virus (MV). MV-infected and uninfected peripheral blood mononuclear cells were cultured with phorbol 2-myristate 13-acetate plus ionomycin in the presence of monensin for 10 hr. Surface antigen and intracellular cytokines, interleukin (IL)-2, interferon (IFN)-gamma, and IL-4, were stained simultaneously and analyzed by flow cytometry. The percentage of cells that expressed IL-2 and IFN-gamma was significantly increased in MV-infected CD3+, CD4+, and CD8+ lymphocytes and alphabeta T lymphocytes compared with that in uninfected lymphocytes. In gammadelta T lymphocytes, expression of IFN-gamma, not IL-2, was increased in MV-infected cells compared with that in uninfected cells. IL-2 was increased mainly in MV-infected CD4+ lymphocytes and alphabeta T lymphocytes, whereas IFN-gamma was increased mainly in MV-infected CD8+ lymphocytes and gammadelta T lymphocytes. Expression of IL-4 was unaffected by MV infection. These results demonstrate that MV enhances intracellular levels of type 1 cytokines during the acute phase of measles.
authors.affiliation: Department of Pediatrics, Mie University School of Medicine, Tsu, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Kamiya
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis; D000956:Antigens, Viral / Q000096:biosynthesis; D002472:Cell Transformation, Viral; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000378:metabolism*; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D007425:Intracellular Membranes / Q000737:chemistry*; D007963:Leukocytes, Mononuclear / Q000821:virology; D016131:Lymphocyte Subsets / Q000737:chemistry* / Q000821:virology; D008457:Measles / Q000473:pathology*; D008459:Measles virus / Q000276:immunology / Q000502:physiology*; D014709:Vero Cells
chemical_list: D000954:Antigens, Surface; D000956:Antigens, Viral; D016207:Cytokines; D007376:Interleukin-2; D007371:Interferon-gamma