Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax.
Six goats were experimentally infected with a stock of Trypanosoma vivax. Parasitaemia was weekly monitored by buffy coat and wet blood film examination during a period of 15 weeks and another 3 weeks following drug-treatment. Dried blood samples were tested by the polymerase chain reaction (PCR), using an extraction method with Chelex 100 (BioRad). PCR proved consistently more sensitive than the parasitological techniques.
de Almeida
P J
PJ
Institute of Tropical Medicine, Belgium. palmeid@itg.be
Ndao
M
M
Van Meirvenne
N
N
Geerts
S
S
D000818:Animals; D005260:Female; D015511:Goat Diseases / Q000175:diagnosis* / Q000469:parasitology; D006041:Goats / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods; D016689:Trypanosoma vivax; D014353:Trypanosomiasis, African / Q000175:diagnosis / Q000662:veterinary*