A functional neo-centromere formed through activation of a latent human centromere and consisting of non-alpha-satellite DNA.
We recently described a human marker chromosome containing a functional neo-centromere that binds anti-centromere antibodies, but is devoid of centromeric alpha-satellite repeats and derived from a hitherto non-centromeric region of chromosome 10q25. Chromosome walking using cloned single-copy DNA from this region enabled us to identify the antibody-binding domain of this centromere. Extensive restriction mapping indicates that this domain has an identical genomic organization to the corresponding normal chromosomal region, suggesting a mechanism for the origin of this centromere through the activation of a latent centromere that exists within 10q25.
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Murdoch Institute for Research into Birth Defects, Royal Children's Hospital, Parkville, Australia.
Cancilla
M R
MR
Earle
E
E
Mao
J I
JI
Saffery
R
R
Tainton
K M
KM
Kalitsis
P
P
Martyn
J
J
Barry
A E
AE
Choo
K H
KH
D001666:Binding Sites, Antibody; D002503:Centromere; D018244:Chromosomes, Artificial, Yeast; D002879:Chromosomes, Human, Pair 10; D004276:DNA, Satellite / Q000235:genetics* / Q000378:metabolism; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence
D004276:DNA, Satellite; D005819:Genetic Markers