title: A calcium-dependent nitric oxide synthase and NMDA R1 glutamate receptor in the ink gland of Sepia officinalis: a hint to a regulatory role of nitric oxide in melanogenesis?
abstract: Histochemical, immunohistochemical, and biochemical evidence is reported showing that the ink gland of the cuttlefish Sepia officinalis contains a calcium-dependent isoform of nitric oxide synthase as well as an NMDA R1 receptor subunit localized for the most part in the immature inner cells of the epithelial layer of the gland. These results may be taken to implicate a hitherto unrecognized regulatory role of the glutamate-nitric oxide pathway in the maturation and metabolic activity of melanin-producing cells in the cephalopod defense system.
authors.affiliation: Stazione Zoologica Anton Dohrn, Naples, Italy. palumbo@alpha.szn.it
authors.forename: A
authors.initials: A
authors.lastname: Palumbo
authors.forename: A
authors.initials: A
authors.lastname: Di Cosmo
authors.forename: I
authors.initials: I
authors.lastname: Gesualdo
authors.forename: M
authors.initials: M
authors.lastname: d'Ischia
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005088:Exocrine Glands / Q000201:enzymology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D018698:Glutamic Acid / Q000378:metabolism; D006651:Histocytochemistry; D007527:Isoenzymes / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis*; D018613:Microscopy, Confocal; D008974:Mollusca / Q000201:enzymology / Q000378:metabolism*; D009252:NADPH Dehydrogenase; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D007527:Isoenzymes; D008543:Melanins; D016194:Receptors, N-Methyl-D-Aspartate; D009569:Nitric Oxide; D018698:Glutamic Acid; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase; D002118:Calcium; D019331:NG-Nitroarginine Methyl Ester