Identification and partial characterization of a myosin-like protein from cysticerci and adults of Taenia solium using a monoclonal antibody.
The host-parasite relationship in taeniosis due to Taenia solium is practically unknown. Monoclonal antibodies were prepared against whole extracts of adult T. solium parasites and evaluated with tapeworms recovered from experimentally infected hamsters and with cysticerci from naturally infected pigs. With one antibody, mAb 4B3, it was possible to identify, purify and partially characterize a T. solium myosin. Some findings indicate that it corresponds to conventional myosin or myosin type II such as: purification with KCl, high molecular weight, size, structure (dimeric protein with globular and long tail portions), reaction with commercial anti-myosin antibodies, distribution in muscle fibres of parasites and cross-reactivity with antibodies against paramyosin from T. solium cysticerci. The reaction of the mAb was only with taeniids and not with other parasites. Also myosin was detected in faeces of infected animals and in supernatants of parasite cultures. Its presence in biological fluids may be useful for diagnosis of infected hosts.
Ambrosio
J
J
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, DF. jrah@servidor.unam.mx
Cruz-Rivera
M
M
Allan
J
J
Morán
E
E
Ersfeld
K
K
Flisser
A
A
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis*; D006224:Cricetinae; D003552:Cysticercus / Q000737:chemistry*; D005260:Female; D015801:Helminth Proteins / Q000032:analysis; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D009218:Myosins / Q000032:analysis*; D013552:Swine; D013621:Taenia / Q000737:chemistry*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D015801:Helminth Proteins; D009218:Myosins