title: The bovine parainfluenza virus type-3 (BPIV-3) hemagglutinin/neuraminidase glycoprotein expressed in baculovirus protects calves against experimental BPIV-3 challenge.
abstract: Despite the availability of numerous vaccine schedules, "shipping fever", an acute bronchopneumonia brought on in part by a complex of bovine respiratory viruses, remains a major source of economic loss in the beef and dairy industries. We are exploring new strategies of bovine vaccine design which we hope may provide more effective and more cost-efficient control of these pathogens. In this report, we examined the possible use of subunit vaccines, using as an example the hemagglutinin/neuraminidase (HN) protein of bovine parainfluenza virus type-3 (BPIV-3) expressed in the baculovirus expression system. We showed that the protein was expressed at high levels, and was modified to a similar, but not identical size as the native HN protein expressed from BPIV-3 infected bovine cells. We further demonstrated antigenicity and biological activity of the expressed HN protein. Finally, we vaccinated colostrum deprived sera-negative calves with the baculo HN recombinant protein and challenged with BPIV-3. Vaccination induced excellent serum neutralizing antibody responses, and surprisingly, good mucosal antibody responses, even though the vaccine was administered parenterally. The vaccinated animals were well protected against challenge.
authors.affiliation: Pharmacia & Upjohn Inc., Kalamazoo, MI 49001, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Haanes
authors.forename: P
authors.initials: P
authors.lastname: Guimond
authors.forename: R
authors.initials: R
authors.lastname: Wardley
mesh_terms: D000818:Animals; D016367:Baculoviridae; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D002460:Cell Line; D015870:Gene Expression; D005822:Genetic Vectors; D015707:HN Protein / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D018108:Respirovirus / Q000276:immunology*; D010253:Respirovirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D018411:Spodoptera / Q000166:cytology; D014611:Vaccination / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D015707:HN Protein; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D006923:Hymecromone; C021224:2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid