A specific assay for discriminating between peroxidase and lipoxygenase activities.
Peroxidases are widespread heme-containing enzymes able to catalyze the oxidation of a large array of organic substrates. There is growing interest in the measurements of peroxidase activity. We noticed that many substrates used in the routine assays for the biological and cytological determinations of peroxidase could be oxidized by lipoxygenase. We found interesting to set up a procedure to detect selectively peroxidase. In the present note, we report a fluorometric test for peroxidase detection using phenolic compounds or hydroxycoumarins.
Fontana
M
M
Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Università La Sapienza, Roma, Italia.
Costa
M
M
Mosca
L
L
Rosei
M A
MA
D000595:Amino Acid Sequence; D006719:Homovanillic Acid / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D006923:Hymecromone / Q000378:metabolism; D007700:Kinetics; D008084:Lipoxygenase / Q000032:analysis / Q000378:metabolism*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism*; D010636:Phenols; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
D010636:Phenols; D006923:Hymecromone; D006861:Hydrogen Peroxide; D009195:Peroxidase; D008084:Lipoxygenase; D006719:Homovanillic Acid