Specific targeting of human papillomavirus type 16 E7 oncogene with triple-helix forming purine oligodeoxyribonucleotides.
Molecular mechanical calculations (computer modelling), optical DNA melting experiments and co-migration assay were used to assess stable helix formation at homopurine--homopyrimidine-rich target sites present in the human papillomavirus type 16 E7 oncogene (positions 656-673 on the genome map). The target sequence, either present in the E7 oncogene obtained by PCR technique or prepared from oligodeoxyribonucleotides (ODNs), can be specifically recognised by different 17-merpurine ODNs designed to form antiparallel or parallel triple helices. These in vitro experiments realised with rather long purine ODNs having a high degree of specificity open the way for in vivo tests focused on E7 oncogene targeting and suppression.
Popa
L M
LM
Medical and Pharmaceutical University of Cluj-Napoca, Romania.
Schütz
H
H
Winter
S
S
Kittler
L
L
Löber
G
G
D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry*; D005813:Genes, Synthetic; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides