Brain creatine kinase reaction rates and reactant concentrations during seizures in developing rats.
Brain creatine kinase (CK) catalyzed phosphorus fluxes between phosphocreatine (PCr) and ATP and changes in reactant concentrations were measured using [31P] nuclear magnetic resonance spectroscopy ([31P]NMR) before and during pentylenetetrazole-induced seizures in 7 and 21 day old rats. The CK rate constants measured before seizures were three times higher in the older than in the younger rats. The rate constants increased 60% during seizures in the older rats but did not change or decreased in the younger. Small decreases in PCr were seen during seizures at both ages. A small decrease in ATP was seen at 7 days but not at 21 days.
Holtzman
D
D
Department of Neurology, Children's Hospital, Boston, MA, USA.
Meyers
R
R
Khait
I
I
Jensen
F
F
D000255:Adenosine Triphosphate / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000254:growth & development*; D001923:Brain Chemistry / Q000502:physiology*; D003402:Creatine Kinase / Q000378:metabolism*; D004569:Electroencephalography; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D010433:Pentylenetetrazole; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*
D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D010433:Pentylenetetrazole