[A new method of ex vivo whole blood perfusion of isolated mammalian organs, exemplified by the kidney of swine].
A new method for the ex vivo perfusion of organs from large mammals is described. Gas exchange and dialysis are carried out simultaneously with a low-flux polysulfon dialysis module. The dialysate (e.g. Tyrode solution) is aerated with a mixture of oxygen and carbon dioxide to ensure gas exchange with the blood. Dialysis is carried out in a closed thermostatically controlled system. Monitoring of ultrafiltration is maintained by continuously weighing the blood reservoir and adjusting an afferent and efferent blood pump. Initial results obtained with isolated pig kidneys demonstrate the suitability of the new method for use as a model for the replacement of animal experiments. Theoretically, clinical application in the area of in vivo regional organ perfusion may also be possible.
von Baeyer
H
H
Universitätsklinikum Rudolf Virchow der Humboldt Universität zu Berlin.
Stahl
K
K
Häusler
M
M
Meissler
M
M
Unger
V
V
Frank
J
J
Grosse-Siestrup
C
C
Kaczmarczyk
G
G
Affeld
K
K
Flaig
H J
HJ
Steinbach
B
B
D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D010478:Chemotherapy, Cancer, Regional Perfusion / Q000295:instrumentation; D015314:Dialysis Solutions; D004867:Equipment Design; D007668:Kidney / Q000098:blood supply*; D010100:Oxygen / Q000097:blood; D010477:Perfusion / Q000295:instrumentation*; D006435:Renal Dialysis / Q000295:instrumentation*; D013552:Swine
D015314:Dialysis Solutions; D002245:Carbon Dioxide; D010100:Oxygen