Metabolic changes associated with vacuolation in murine models of scrapie using in vitro 1H-NMR spectroscopy.
In this study, metabolic changes in the 79A/C3H, ME7/VM, ME7/C3H, 87V/VM and 22A/SV scrapie mouse models were investigated during the clinical phase of the disease, using in vitro proton nuclear magnetic resonance spectroscopy. N-acetyl-aspartate was found to be significantly reduced in infected mice of the ME7/ C3H (40% reduction), ME7/VM (26%) and 79A/C3H (17%) models when compared to control mice, but not in the 87V/VM and 22A/SV models. The concentration of choline containing compounds and creatine remain unchanged in all models when compared with control murine brains. The level of N-acetyl-aspartate reduction correlated with the extent of grey-matter brain vacuolation. The levels of myo-inositol were found to be significantly increased in the ME7/VM (143%) and 79A/C3H (132%) models only and no significant changes were observed in the ME7/C3H, 87V/VM and 22A/SV models. These changes did not correspond to the severity of gliosis.
Chung
Y L
YL
Robert Steiner MR Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Williams
A
A
Chong
A
A
Hope
J
J
Williams
S C
SC
Bell
J D
JD
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D012016:Reference Values; D012608:Scrapie / Q000175:diagnosis / Q000378:metabolism*; D014617:Vacuoles / Q000473:pathology* / Q000648:ultrastructure
D001224:Aspartic Acid; D006859:Hydrogen; C000179:N-acetylaspartate