Membrane topology of the metal-tetracycline/H+ antiporter TetA(K) from Staphylococcus aureus.
A series of fusions to the reporter proteins alkaline phosphatase and beta-galactosidase have been constructed in the predicted periplasmic and cytoplasmic loops of TetA(K), a protein responsible for efflux-mediated tetracycline resistance in Staphylococcus aureus. The results support a topological model of 14 transmembrane segments for TetA(K).
Ginn
S L
SL
School of Biological Sciences, University of Sydney, New South Wales, Australia.
Brown
M H
MH
Skurray
R A
RA
D000595:Amino Acid Sequence; D017920:Antiporters / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002462:Cell Membrane / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D013211:Staphylococcus aureus / Q000737:chemistry*
D017920:Antiporters; D001426:Bacterial Proteins; C079051:tetA protein, Bacteria