Expression of a highly basic peroxidase gene in NaCl-adapted tomato cell suspensions.
A tomato peroxidase gene, TPX2, that is only weakly expressed in the roots of young tomato seedlings is highly expressed in tomato suspension cells adapted to high external NaCl concentration. The protein encoded by this gene, with an isolectric point value of approximately 9.6, is found in the culture medium of the growing cells. Our data suggest that the expression of TPX2 in the salt-adapted cells is not the result of the elicitation imposed by the in vitro culture or the presence of high NaCl concentration in the medium.
Medina
M I
MI
Departamento de Bioquímica y Biología Molecular, Universidad de Málaga, Spain.
Botella
M A
MA
Quesada
M A
MA
Valpuesta
V
V
D000222:Adaptation, Physiological; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007526:Isoelectric Point; D018551:Solanum lycopersicum / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D010544:Peroxidases / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride
D012333:RNA, Messenger; D018749:RNA, Plant; D012965:Sodium Chloride; D010544:Peroxidases