Effect of fixation on interphase cytogenetic analysis by direct fluorescence in situ hybridization on cell imprints.
The direct fluorescent in situ hybridization (FISH) technique using a centromerespecific DNA probe for chromosome 11 was applied on fresh tissue imprints from melanomas and pituitary adenomas. The cell imprints were fixed in acetone and formalin, and the influence of fixation was evaluated. Acetone fixed imprints gave a sharp and intense fluorescent signal in a large number of cells from both tumors without any pretreatment. In contrast, formalin fixed imprints disclosed a weak hybridization signal in a few cells even after careful enzymatic digestion. Direct FISH on acetone fixed cell imprints represents a simple and time saving technique applicable to any laboratory for the study of numerical chromosomal abnormalities.
Kapranos
N
N
Department of Pathology, Hygeia Hospital, General Hospital of Athens, Greece.
Kontogeorgos
G
G
Frangia
K
K
Kokka
E
E
D000096:Acetone; D000236:Adenoma / Q000235:genetics; D002880:Chromosomes, Human, Pair 11; D003582:Cytogenetics / Q000379:methods*; D004273:DNA, Neoplasm / Q000032:analysis; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D008545:Melanoma / Q000235:genetics; D010911:Pituitary Neoplasms / Q000235:genetics; D016707:Tissue Fixation / Q000379:methods
D004273:DNA, Neoplasm; D005404:Fixatives; D000096:Acetone; D005557:Formaldehyde