A monoclonal antibody directed against the 97-kilodalton gonococcal hemin-binding protein inhibits hemin utilization by Neisseria gonorrhoeae.
Neisseria gonorrhoeae expresses two hemin-binding proteins (HmBPs) of 97,000 and 44,000 in molecular weight. A murine monoclonal antibody (MAb) produced against the 97-kDa HmBP from N. gonorrhoeae PID543 specifically inhibited in a concentration-dependent manner the ability of hemin to promote growth. The anti-97-kDa HmBP MAb competitively inhibited binding of the 97-kDa HmBP to a hemin-agarose affinity column. In Western immunoblots, the MAb recognized the 97-kDa homologs from a limited survey of clinical gonococcal isolates. These results support the contention that the 97-kDa HmBP is involved in the gonococcal hemin acquisition pathway.
Lee
B C
BC
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada. leec@acs.ucalgary.ca
Levesque
S
S
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology / Q000502:physiology*; D042966:Cytochrome-B(5) Reductase / Q000276:immunology / Q000502:physiology*; D000081062:Heme-Binding Proteins; D006420:Hemeproteins / Q000276:immunology / Q000502:physiology*; D006427:Hemin / Q000378:metabolism*; D009344:Neisseria gonorrhoeae / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D006427:Hemin; D042966:Cytochrome-B(5) Reductase