title: Optimization of haemolysis in enhanced haemolysis agar (EHA)--a selective medium for the isolation of Listeria monocytogenes.
abstract: The presence of Listeria monocytogenes in enrichment media can be masked by faster growth of other Listeria spp. Therefore, enhanced haemolysis agar (EHA) is a good alternative for another isolation media, because the presence of a few L. monocytogenes colonies can be detected in a majority of colonies of other listeriae on the basis of haemolysis. In this study the haemolysis reaction in EHA was optimized. In a collaborative study using reference samples, no significant differences in counts on EHA, Palcam and Oxford agar were shown.
authors.affiliation: Laboratory of Food Microbiology and Hygiene, Agricultural University, Wageningen, The Netherlands.
authors.forename: R R
authors.initials: RR
authors.lastname: Beumer
authors.forename: M C
authors.initials: MC
authors.lastname: te Giffel
authors.forename: L J
authors.initials: LJ
authors.lastname: Cox
mesh_terms: D000362:Agar; D001431:Bacteriological Techniques / Q000592:standards; D015169:Colony Count, Microbial; D005069:Evaluation Studies as Topic; D006461:Hemolysis; D008089:Listeria monocytogenes / Q000254:growth & development / Q000302:isolation & purification*; D012015:Reference Standards
chemical_list: D000362:Agar