title: The malEFG gene cluster of Streptomyces coelicolor A3(2): characterization, disruption and transcriptional analysis.
abstract: The malEFG gene cluster of the Gram-positive mycelial actinomycete Streptomyces coelicolor A3(2) was cloned and sequenced. MalEFG show only limited similarity to homologues involved in maltose and maltodextrin transport in other bacteria. Disruption of malE prevented the utilization of maltose as carbon source. Transcription of malE was induced by maltose and repressed by glucose.
authors.affiliation: Department of Genetics, John Innes Centre, Norwich Research Park, Colney, UK.
authors.forename: G P
authors.initials: GP
authors.lastname: van Wezel
authors.forename: J
authors.initials: J
authors.lastname: White
authors.forename: M J
authors.initials: MJ
authors.lastname: Bibb
authors.forename: P W
authors.initials: PW
authors.lastname: Postma
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D005798:Genes, Bacterial; D005947:Glucose / Q000494:pharmacology; D008320:Maltose / Q000378:metabolism*; D058619:Maltose-Binding Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C079123:MalG protein, E coli; D058619:Maltose-Binding Proteins; D008320:Maltose; D005947:Glucose