title: Ultrastructural morphometry of parotid acinar cells following fractionated irradiation.
abstract: The aim of this study was to evaluate the long term effects on the ultrastructure of parotid glands after fractionated irradiation. The method implemented involved 5 x 6 Gy and 5 x 8 Gy, Monday to Friday 6 MV photons. By unilateral irradiation, the contralateral parotid gland served as a control. Although irradiation diminished the acinar cell density in light microscopic sections from 75 to 32% after 5 x 8 Gy of irradiation, ultrastructural morphometry could not detect any statistically significant differences in acinar cell size, nuclear size, nuclear density, granule area, mean granule size, or granule density. In general, greater differences were seen between rats receiving 30 or 40 Gy, on both the irradiated and the control size, than between the irradiated side and the control side. This was interpreted as due to differences in the nutritional state of the animals. This analysis concluded that individual acinar cells that survive irradiation seem not to be damaged in the long term when evaluated at the ultrastructural level. The study further stresses the importance of adequate sampling sizes and the use of adequate controls.
authors.affiliation: Department of Otorhinolaryngology-head & Neck Surgery, Umeå University, Sweden.
authors.forename: A L
authors.initials: AL
authors.lastname: Grehn
authors.forename: H
authors.initials: H
authors.lastname: Gustafsson
authors.forename: L
authors.initials: L
authors.lastname: Franzén
authors.forename: L E
authors.initials: LE
authors.lastname: Thornell
authors.forename: R
authors.initials: R
authors.lastname: Henriksson
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016371:Cranial Irradiation / Q000009:adverse effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008854:Microscopy, Electron; D010306:Parotid Gland / Q000528:radiation effects* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley