Quantitation of beta-lapachone and 3-hydroxy-beta-lapachone in human plasma samples by reversed-phase high-performance liquid chromatography.
beta-Lapachone is an o-naphthoquinone found to have in vitro cytotoxicity in cancer cells, type I human immunodeficiency virus, and fungi. Analytical methods for evaluating beta-lapachone in biological fluids using high-performance liquid chromatography (HPLC) have not been published. The reversed-phase HPLC method described in this report utilizes liquid extraction of a 0.5-ml plasma sample with average recoveries of 67+/-10.8% and 70+/-10.3% for beta-lapachone and 3-hydroxy-beta-lapachone, respectively. Sensitivity of the assay using ultraviolet (UV) detection at 256 nm is 15 ng ml(-1) from a 100 microl injection. Plasma standards for beta-lapachone and 3-hydroxy-beta-lapachone are linear with no significant difference in slope between the compounds. The retention times of 2.7 min for 3-hydroxy-beta-lapachone and 5.9 min for beta-lapachone result in a clean separation permitting use of the same assay procedure without modification for both compounds. This assay offers the advantage that either beta-lapachone or 3-hydroxy-beta-lapachone can serve as the internal standard, depending on which compound is being analyzed.
Glen
V L
VL
School of Pharmacy, University of Wisconsin, Madison, USA.
Hutson
P R
PR
Kehrli
N J
NJ
Boothman
D A
DA
Wilding
G
G
D000890:Anti-Infective Agents / Q000097:blood*; D000903:Antibiotics, Antineoplastic / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009285:Naphthoquinones / Q000097:blood*
C106995:3-hydroxylapachone; D000890:Anti-Infective Agents; D000903:Antibiotics, Antineoplastic; D009285:Naphthoquinones; C014638:beta-lapachone