Interleukin-18 activates NF-kappaB in murine T helper type 1 cells.
Interleukin-18 (IL-18) activated T helper type 1 (Th1) cells, OVA#4, and induced production of interleukin-2 (IL-2) in costimulation with anti-CD3 antibody. Upon stimulation with IL-18, IkappaB disappeared from cytoplasm and subsequently nuclear factor-kappaB (NF-kappaB) (p65) accumulated in the nucleus. Corresponding with that, DNA binding activity of NF-kappaB (p65 homodimer or p65/p50 heterodimer) was detected in the nucleus. In the transfection experiments, an IL-2 promoter-driven reporter construct showed the similar responsiveness against IL-18 to that of the intrinsic IL-2 gene, and a construct lacking kappaB site failed to respond to IL-18. These results suggest that IL-18 activates NF-kappaB and it is important for enhancement of IL-2 gene expression by Th1 cells stimulated with IL-18.
Matsumoto
S
S
Fujisaki Institute, Hayashibara Biochemical Labs, Inc., Okayama, Japan.
Tsuji-Takayama
K
K
Aizawa
Y
Y
Koide
K
K
Takeuchi
M
M
Ohta
T
T
Kurimoto
M
M
D000818:Animals; D001483:Base Sequence; D001693:Biological Transport, Active; D002467:Cell Nucleus / Q000378:metabolism; D002999:Clone Cells; D016207:Cytokines / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D015342:DNA Probes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D017930:Genes, Reporter; D020672:I-kappa B Proteins; D020382:Interleukin-18; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism*; D015533:Transcriptional Activation; D014162:Transfection
D016207:Cytokines; D015342:DNA Probes; D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; D020382:Interleukin-18; D007376:Interleukin-2; D016328:NF-kappa B; C000606206:Nfkbia protein, rat; D000072000:NF-KappaB Inhibitor alpha; D004247:DNA