Restriction endonuclease analysis of DNA from Indian isolates of bovine herpesvirus 1.
The genomic variation of three isolates of bovine herpes virus 1 (BHV-1) originating from different geographical regions and displaying different clinical symptoms were studied by restriction analysis using four different restriction endonucleases. EcoRI displayed an uniform restriction pattern for all the isolates and HindIII showed marginal variation among the isolates. BstEII and PstI displayed unique restriction patterns, based on which the isolates could be grouped into two subgroups of BHV-1.1. BstEII appears to be the enzyme of choice for differentiating BHV-1.1.
Sreenivasa
B P
BP
Indian Veterinary Research Institute, Bangalore Campus, Hebbal, India.
Natarjan
C
C
Rasool
T J
TJ
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000302:isolation & purification; D007194:India
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C032823:GGTNACC-specific type II deoxyribonucleases