title: Changes in fatty acid profiles in different lipid classes during late development of turkey embryos from two genetic lines.
abstract: Fatty acid (FA) profiles in embryonic yolk sacs (YS) and livers were studied in embryos from a randombred turkey line (RBC2) and a line selected for body weight at 16 wk (F line). There were no differences in FA profiles of fresh yolk lipids. During the course of incubation, oleic acid (C18:1) was higher and linoleic acid (C18:2) was lower in YS triglyceride (TG) and phospholipid (PL) subclasses in F line compared with RBC2 embryos. In both lines, the C18:1 content of YS cholesteryl esters (CE) increased from 58 to 63% during the last 6 d of incubation. From 22 to 28 d of incubation, there was a constant C18:1 concentration in hepatic CE, which was > 60% of total hepatic CE FA. As incubation proceeded, palmitic acid (C16:0) and C18:1 in hepatic TG decreased from 27 to 16% and 37 to 34%, respectively. The stearic acid (C18:0) in TG increased from 12% at Day 22 to 32% of total FA at hatch (Day 28) in RBC2 embryos compared with a lesser increase in the F line (11.8 to 18.6%). In hepatic PL, arachidonic acid (C20:4) decreased, whereas both C16:0 and C18:0 increased from 22 to 28 d of incubation. During this same time period, there was an overall decline in docosahexaenoic acid (C22:6) only in the RBC2. On Days 26 and 28, F line embryos had greater concentrations of C22:6 and C20:4 in hepatic PL than did RBC2. These results suggest that selection for increased BW has changed the proportional incorporation of different FA into embryonic lipids.
authors.affiliation: Department of Animal Sciences, Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691-4096, USA.
authors.forename: S T
authors.initials: ST
authors.lastname: Ding
authors.forename: M S
authors.initials: MS
authors.lastname: Lilburn
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000032:analysis / Q000378:metabolism; D002788:Cholesterol Esters / Q000737:chemistry* / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000032:analysis / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D005260:Female; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000737:chemistry* / Q000196:embryology / Q000378:metabolism; D019301:Oleic Acid / Q000032:analysis / Q000378:metabolism; D019308:Palmitic Acid / Q000032:analysis / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D013229:Stearic Acids / Q000032:analysis / Q000378:metabolism; D014280:Triglycerides / Q000737:chemistry* / Q000378:metabolism; D014422:Turkeys / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D015017:Yolk Sac / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001095:Arachidonic Acids; D002788:Cholesterol Esters; D005227:Fatty Acids; D008041:Linoleic Acids; D010743:Phospholipids; D013229:Stearic Acids; D014280:Triglycerides; D004281:Docosahexaenoic Acids; D019301:Oleic Acid; D019308:Palmitic Acid; D019787:Linoleic Acid