A complementary DNA encoding an antigen from Trichinella spiralis muscle larvae and its analog from Trichinella T5 of bobcat origin: sequence, cloning and expressions.
Reverse transcription-polymerase chain reaction (RT-PCR) was employed to amplify a cDNA encoding an excretory-secretory (ES) antigen with mol. wt 45-50 kDa by SDS-PAGE from T. spiralis muscle larvae. The PCR product was purified by electrophoresis and sequenced by thermal cycle sequencing with primer walking. The cDNA is 890 bp long and encodes a polypeptide of 255 amino acid (AA) residues. Using the same methods, we also recovered a corresponding cDNA from Trichinella T5, which is 891 bp long and encodes 255 AAs. Comparison of the 2 Trichinella species indicates approximately 2.6% and 2.4% differences between the 2 cDNA sequences and between the 2 deduced AA sequences, respectively.
Yao
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Department of Parasitology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
McGraw
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RA
Prestwood
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AK
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics*; D001483:Base Sequence; D002335:Carnivora / Q000469:parasitology*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018509:DNA, Helminth; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D015801:Helminth Proteins / Q000235:genetics*; D007814:Larva; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000469:parasitology; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D013552:Swine; D014234:Trichinella / Q000235:genetics* / Q000276:immunology; D017160:Trichinella spiralis / Q000235:genetics* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins; C097419:excretory-secretory antigen, Trichinella