title: Analysis of molecular forms and pharmacological properties of acetylcholinesterase in several mosquito species.
abstract: Two acetylcholinesterases (AChE1 and AChE2) have recently been characterized in the common mosquito Culex pipiens. This situation appeared to be an exception among insects, where only one acetylcholinesterase gene had previously been repeatedly reported. In the present study, acetylcholinesterase was studied in five mosquito species: Aedes aegypti, Anopheles gambiae, Anopheles stephensi, Culiseta longeareolata and Culex hortensis, in order to test whether or not two different acetylcholinesterase enzymes could be detected as occurs in C. pipiens. Molecular forms and catalytic properties of the enzyme show that only one enzyme species was detected in the five species. This suggests that a duplication of a single locus Ace probably occurred recently in the phylogeny tree leading to C. pipiens, and produced two distinct acetylcholinesterases: AchE1 and AChE2.
authors.affiliation: Laboratoire Génétique et Environment, Université Montpellier-II, France.
authors.forename: D
authors.initials: D
authors.lastname: Bourguet
authors.forename: A
authors.initials: A
authors.lastname: Roig
authors.forename: J P
authors.initials: JP
authors.lastname: Toutant
authors.forename: M
authors.initials: M
authors.lastname: Arpagaus
mesh_terms: D000110:Acetylcholinesterase / Q000494:pharmacology*; D000330:Aedes / Q000201:enzymology; D000818:Animals; D000852:Anopheles / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D003465:Culex / Q000201:enzymology; D009033:Culicidae / Q000201:enzymology*; D007527:Isoenzymes / Q000494:pharmacology*; D013045:Species Specificity
chemical_list: D002800:Cholinesterase Inhibitors; D007527:Isoenzymes; D000110:Acetylcholinesterase