High glucose and hyperosmolarity increase secretion of interleukin-1 beta in cultured human aortic endothelial cells.
Interleukin-1 (IL-1) is secreted by endothelial cells (ECs) and smooth-muscle cells (SMCs), which are two major component cells of vessels and detected in atherosclerotic lesions. To evaluate the effect of hyperglycemia on the secretion of IL-1 beta in endothelial cells in diabetic patients, we investigated the effects of high glucose and hyperosmolar conditions on the secretion of IL-1 beta from cultured human aortic endothelial cells (HAECs). HAECs were treated with high concentration of glucose or hyperosmolar condition for 3 days. IL-1 beta in the supernatant was measured by high sensitive enzyme-linked immunosorbent assay (ELISA). Under high concentration of glucose (16.6 mmol/L) and hyperosmolar condition (glucose 5.5 mmol/L + mannitol 11.1 mmol/L), the secretion of IL-1 beta was significantly increased (41.0 +/- 2.8 and 26.3 +/- 5.9% increase, respectively, compared with that of 5.5 mmol/L glucose). In conclusion, high glucose and hyperosmolar condition increase the secretion of IL-1 beta in HAECs. The results suggest that diabetic macroangiopathies might be accelerated partly through the increase of IL-1 beta secretion in HAECs.
Asakawa
H
H
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Miyagawa
J
J
Hanafusa
T
T
Kuwajima
M
M
Matsuzawa
Y
Y
D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology / Q000378:metabolism*; D008353:Mannitol / Q000494:pharmacology; D009994:Osmolar Concentration; D011933:Reagent Kits, Diagnostic; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D017077:Culture Media, Conditioned; D007375:Interleukin-1; D011933:Reagent Kits, Diagnostic; D014409:Tumor Necrosis Factor-alpha; D008353:Mannitol; D005947:Glucose