title: Rapid preparation method for technetium-99m bicisate.
abstract: The method currently recommended for the preparation of technetium-99m bicisate (99mTc-bicisate) requires a lengthy 30-min incubation at room temperature. The purpose of this study was to evaluate an alternative method to shorten the preparation time. 99mTc-bicisate was prepared with 3.7 GBq (100 mCi) 99mTc according to the manufacturer's instructions, except for the final incubation step, which was replaced with the microwave heating procedure. A standard thin-layer chromatography (TLC) method (i.e., Baker-Flex silica gel IB-F TLC plate with ethyl acetate as mobile phase) was used for the determination of the radiochemical purity (RCP) of 99mTc-bicisate. Our evaluation with different microwave heating processes (300 W with different heating times) demonstrated that as the microwave heating temperature was increased (i.e., 44 masculine-71 masculine C), an increased percentage of samples reached 95% within 5 min post preparation (n=58). The highest RCP value (i.e., 97.4%+/-0.5%, n=10) could be obtained immediately after an 8-s microwave heating time at 300 W (microwave temperature at 69 masculine C), and an average RCP value of 96.4%+/-1.3% (n=90) was maintained throughout the 24-h evaluation period. However, the trend seemed to reverse at higher microwave temperatures (i.e., 76 masculine-90 masculine C), which reconfirmed our initial findings that overheating had no benefit for the preparation of 99mTc-bicisate. To ensure that temperature was the only determining factor, a hot water incubator set at 69 masculine C was used (n=6). Similar RCP results were achieved. In conclusion, the use of a microwave oven at a low heat cycle provides a rapid and efficient way to prepare 99mTc-bicisate.
authors.affiliation: Nuclear Medicine, Department of Diagnostic Radiology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Hung
authors.forename: S
authors.initials: S
authors.lastname: Chowdhury
authors.forename: M G
authors.initials: MG
authors.lastname: Redfern
authors.forename: D W
authors.initials: DW
authors.lastname: Mahoney
mesh_terms: D002855:Chromatography, Thin Layer; D003545:Cysteine / Q000031:analogs & derivatives*; D006358:Hot Temperature; D006801:Humans; D008872:Microwaves; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D011933:Reagent Kits, Diagnostic; D013997:Time Factors
chemical_list: D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D011933:Reagent Kits, Diagnostic; C059659:technetium Tc 99m bicisate; D003545:Cysteine