5'-RNA self-capping from guanosine diphosphate.
A selected RNA (isolate 6) efficiently catalyzes a self-capping reaction with free GDP, yielding the same 5'-capped structure as is formed by protein GTP:RNA guanylyltransferase. This unexplored RNA-catalyzed reaction type involving nucleophilic attack on phosphate by phosphate adds to the variety of possible postsynthetic RNA-catalyzed RNA modifications. The selected RNA requires only Ca2+ for activation and has a broad active pH range of 4.5-9.0. The RNA also has a 5'-pyrophosphatase activity.
Huang
F
F
Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, 80309-0347, USA.
Yarus
M
M
D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D006153:Guanosine Diphosphate / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012315:RNA Caps / Q000737:chemistry / Q000378:metabolism*; D000079962:RNA, Circular; D012260:Ribonucleases / Q000378:metabolism; D012972:Sodium Hydroxide; D000098556:mRNA Guanylyltransferases
D012315:RNA Caps; D000079962:RNA, Circular; D006153:Guanosine Diphosphate; D012972:Sodium Hydroxide; D012313:RNA; D006160:Guanosine Triphosphate; D009713:Nucleotidyltransferases; D000098556:mRNA Guanylyltransferases; D012260:Ribonucleases; D011755:Pyrophosphatases; D002118:Calcium