title: Detection of antibodies to Bartonella henselae in clinically diagnosed cat scratch disease.
abstract: OBJECTIVE
  To determine the usefulness of an indirect immunoflourescence antibody test for antibodies to Bartonella henselae in diagnosing cat scratch disease (CSD).
  
  
  METHODS
  Retrospective case survey of 354 patients whose sera were tested for antibodies to B. henselae at Royal Perth Hospital, Perth, and the Institute of Clinical Pathology and Medical Research, Sydney. In 1994; and measurement of the background prevalence of antibodies to B. henselae.
  
  
  METHODS
  Prevalence of antibodies to B. henselae, odds of a positive titre (> or = 64) in patients with and without specific risk factors for CSD and clinical features of the disease; prevalence of antibodies to B. henselae in randomly selected blood donors.
  
  
  RESULTS
  Demographic, clinical and cat contact data were available for 303 patients. Sixty-four (21.1%) had a positive titre, as did 53 of 98 (54%) patients with a history of cat contact and lymphadenopathy. This proportion increased to 62% (38 of 61 patients) in patients with a history of cat scratch or bite and to 90.3% (28 of 31) in those with cat contact, lymphadenopathy and histological evidence of granulomatous lymphadenitis. Patients who developed lymphadenopathy after cat contact were significantly more likely to have a positive titre than those without this history (odds ratio [OR], 20.8; 95% confidence interval [95% Cl], 9.6-46; P < 0.0001). Inclusion of a history of a cat scratch or bite significantly raised the odds of being seropositive (OR, 13.7; 95% Cl, 6.8-28.1; P < 0.0001), and the presence of granulomas on lymph node biopsy further increased the odds (OR, 124.4; 95% Cl, 19.4-1073; P < 0.0001). The prevalence of antibodies to B. henselae in random blood donors in New South Wales was about 5% (five of 102 sera samples).
  
  
  CONCLUSIONS
  The immunofluorescence antibody test for B. henselae can be expected to be positive in just over half the patients with clinically suspected CSD, and it has a positive predictive value of 83%. In a significant number of cases the diagnosis cannot be made on the basis of the results of immunofluorescence antibody testing alone and further investigations, including lymph node biopsy, may be required.
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Royal Perth Hospital, WA. jameflex@dunamis.rph.uwa.edu.au
authors.forename: J P
authors.initials: JP
authors.lastname: Flexman
authors.forename: S C
authors.initials: SC
authors.lastname: Chen
authors.forename: D J
authors.initials: DJ
authors.lastname: Dickeson
authors.forename: J W
authors.initials: JW
authors.lastname: Pearman
authors.forename: G L
authors.initials: GL
authors.lastname: Gilbert
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D018416:Bartonella henselae / Q000276:immunology*; D002372:Cat-Scratch Disease / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D002415:Cats; D002648:Child; D002675:Child, Preschool; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D015995:Prevalence; D011897:Random Allocation; D012189:Retrospective Studies; D012307:Risk Factors; D011795:Surveys and Questionnaires
chemical_list: D000907:Antibodies, Bacterial