Determination of dimethylamine in biological samples by high-performance liquid chromatography.
A reversed-phase HPLC method for the quantification of dimethylamine in serum and urine is presented. Dimethylamine (DMA) is converted into a stable fluorescent product by precolumn derivatization with fluorenylmethylchloroformate. The DMA derivative is resolved from derivatives of other amines and amino acids by gradient elution with a total run-time of 15 min. The lower limit of determination in biological samples is 0.1 micromol/l. Recoveries from spiked serum samples were 99-107%. Within- and between-run precision were better than 6%. Concentrations of DMA in serum from normal human subjects (n=8) and from continuous ambulatory peritoneal dialysis patients (n=15) were 3.3+/-1.5 and 29.1+/-12.1 micromol/l, respectively.
Teerlink
T
T
Department of Clinical Chemistry, Free University Hospital, Amsterdam, Netherlands.
Hennekes
M W
MW
Mulder
C
C
Brulez
H F
HF
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D015314:Dialysis Solutions / Q000032:analysis*; D004123:Dimethylamines / Q000097:blood* / Q000652:urine*; D005449:Fluorenes; D006801:Humans; D007202:Indicators and Reagents; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy / Q000652:urine; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015314:Dialysis Solutions; D004123:Dimethylamines; D005449:Fluorenes; D007202:Indicators and Reagents; C054007:1-(9-fluorenyl)methyl chloroformate; C034516:dimethylamine