Efficient assay for the determination of atenolol in human plasma and urine by high-performance liquid chromatography with fluorescence detection.
An efficient method for the determination of atenolol in human plasma and urine was developed and validated. alpha-Hydroxymetoprolol, a compound with a similar polarity to atenolol, was used as the internal standard in the present high-performance liquid chromatographic analysis with fluorescence detection. The assay was validated for the concentration range of 2 to 5000 ng/ml in plasma and 1 to 20 microg/ml in urine. For both plasma and urine, the lower limit of detection was 1 ng/ml. The intra-day and inter-day variabilities for plasma samples at 40 and 900 ng/ml, and urine samples at 9.5 microg/ml were <3% (n=5).
Chiu
F C
FC
Department of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin NT.
Zhang
J N
JN
Li
R C
RC
Raymond
K
K
D000319:Adrenergic beta-Antagonists / Q000097:blood* / Q000652:urine*; D000959:Antihypertensive Agents / Q000097:blood* / Q000652:urine*; D001262:Atenolol / Q000097:blood* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D000319:Adrenergic beta-Antagonists; D000959:Antihypertensive Agents; D001262:Atenolol