Purification and characterization of dexamethasone inducible hepatic cytochrome P450 isozymes from rhesus monkey.
Two isozymes of hepatic cytochrome P450 named DEX M-1 and M-2 have been purified and characterized from dexamethasone (DEX) pretreated (150 mg Kg-1 body wt x 4 days) rhesus monkeys by various chromatographic procedures. These isozymes demonstrated similar peptide maps. Their absolute and CO-dithionite reduced difference spectra demonstrated maximum absorbance at 417 and 449.4 nm, respectively. DEX M-1 and M-2 demonstrated polypeptide molecular wt of 50 and 52.5 KDa, specific content of 16.35 and 11.39 nmol mg-1 protein and 11 and 8 fold purification, respectively. The antibodies against these isozymes cross reacted with each other and also demonstrated slight differences in the immunoinhibition of erythromycin N-demethylase. These results demonstrated that DEX induced two different isozymes of hepatic cytochrome P450 in rhesus monkeys.
Puri
V
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Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Kohli
K K
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D000818:Animals; D000893:Anti-Inflammatory Agents / Q000633:toxicity*; D002845:Chromatography; D003429:Cross Reactions; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D003907:Dexamethasone / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D007106:Immune Sera / Q000276:immunology; D007527:Isoenzymes / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D008253:Macaca mulatta; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D008970:Molecular Weight; D013056:Spectrophotometry, Ultraviolet
D000893:Anti-Inflammatory Agents; D007106:Immune Sera; D007527:Isoenzymes; D003907:Dexamethasone; D003577:Cytochrome P-450 Enzyme System