Can we detect mutagenic activity of urinary sediment by the Ames test?
Using the Salmonella typhimurium strain TA98 we tested the mutagenicity of filtrate and sediment of urine collected from children and coke-oven workers living in the town of Dabrowa Górnicza. Mutagenic substances were detected in samples of urinary filtrate taken either from environmentally exposed children or from occupationally exposed coke-oven workers. The mutagenic effect was found only in acetone extracts of urinary filtrate in the presence of promutagenic activating fraction S9. Beta-glucuronidase/arylsulphatase treatment hydrolysed the conjugates contained urinary filtrate into compounds that were toxic towards tester strain. The mutagenic effect of urine should be tested only in urinary filtrate as we have never detected mutagenic substances in urinary sediment.
Bubak
A
A
Institute of Occupational Medicine and Environmental Health, Sosnowiec, Poland.
Mielzyńska
D
D
Siwińska
E
E
D000328:Adult; D002648:Child; D003077:Coke / Q000009:adverse effects; D004781:Environmental Exposure; D006801:Humans; D016014:Linear Models; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D016273:Occupational Exposure; D011044:Poland; D012486:Salmonella typhimurium / Q000187:drug effects; D018709:Statistics, Nonparametric; D014556:Urine
D003077:Coke; D009153:Mutagens