Suppression of neutral cholesterol ester hydrolase activity by antisense DNA of hormone-sensitive lipase.
In order to investigate the role of the hormone-sensitive lipase (HSL) gene in the activity of neutral cholesterol ester hydrolase (NCEH) from a molecular perspective, Chinese hamster ovary (CHO) cells were transfected with rat antisense hormone-sensitive lipase cDNA, and three different cell lines, designated as anti-HSL, were established. NCEH activity in anti-HSL cells was reduced to approximately 50% of that in control CHO cells. The concentration of cellular esterified cholesterol increased and the concentration of free cholesterol decreased in the anti-HSL cell lines. These results suggest that the HSL gene has a function of NCEH as well.
Osuga
J
J
Third Department of Internal Medicine, University of Tokyo, Japan.
Ishibashi
S
S
Shimano
H
H
Inaba
T
T
Kawamura
M
M
Yazaki
Y
Y
Yamada
N
N
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics; D004951:Esterification; D005822:Genetic Vectors; D051381:Rats; D002787:Sterol Esterase / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D002788:Cholesterol Esters; D017931:DNA Primers; D016373:DNA, Antisense; D018076:DNA, Complementary; D002784:Cholesterol; D002787:Sterol Esterase