Understanding enzymic catalysis: the importance of short, strong hydrogen bonds.
We have proposed previously that short, strong hydrogen bonds exist in enzyme active sites and that they are important in explaining enzymic rate enhancements. Here, we defend this proposal and provide evidence for likely changes of hydrogen bond strengths during enzymic catalysis.
Gerlt
J A
JA
Department of Biochemistry, University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801, USA. j-gerlt@uiuc.edu
Kreevoy
M M
MM
Cleland
W
W
Frey
P A
PA
D001665:Binding Sites; D002384:Catalysis; D002918:Chymotrypsin / Q000737:chemistry; D003562:Cytidine / Q000737:chemistry; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D015394:Molecular Structure; D011522:Protons; D013816:Thermodynamics
D004798:Enzymes; D011522:Protons; D003562:Cytidine; D002918:Chymotrypsin