Distortions of the DNA double helix induced by 1,3-trans-diamminedichloroplatinum(II)-intrastrand cross-link: an internal coordinate molecular modeling study.
A trans-diamminedichloroplatinum(II) (trans-DDP) intrastrand adduct within the sequence d(TCTG*TG*TC).d(GACACAGA) (where G* represents a platinated guanine) is modeled on the basis of qualitative experimental data concerning global unwinding and curvature as well as information on base pairing. Modeling is performed using the internal coordinate JUMNA program, specific to nucleic acids, and modified to include the possibility of covalently bound ligands. Calibration of the energy functions representing the Pt-N7 bond with guanine is described. The platinum atom and the platinum-nitrogen bonds are parameterized for use in the Hückel Del Re method to calculate monopoles at each atom. These monopoles are consistent with the Flex force field included in Jumna. By developing an appropriate minimization protocol we are able to generate stable, distorted three-dimensional structures compatible with the experimental data and including an unusually high global unwinding. No a priori geometric assumptions are made in generating these structures.
Prévost
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C
Centre de Biophysique Moléculaire, CNRS UPR 4301, Orléans, France. chantal@ibpc.fr
Boudvillain
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M
Beudaert
P
P
Leng
M
M
Lavery
R
R
Vovelle
F
F
D000465:Algorithms; D002945:Cisplatin / Q000494:pharmacology*; D003198:Computer Simulation; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D018736:DNA Adducts; D008958:Models, Molecular; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry
D003432:Cross-Linking Reagents; D018736:DNA Adducts; D009838:Oligodeoxyribonucleotides; C034697:transplatin; D004247:DNA; D002945:Cisplatin