Preparation and crystallization of a cross-linked complex of bovine adrenodoxin and adrenodoxin reductase.
Bovine adrenodoxin was cross-linked to adrenodoxin reductase with 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide. Mass spectrometry showed the reaction product to be a 1:1 complex of the two proteins with M(r) = 64,790 +/- 50. The cross-linked complex showed cytochrome c reductase activity and could be crystallized by hanging-drop vapor diffusion. Crystals of the adrenodoxin-adrenodoxin reductase complex are hexagonal, space group P6(1)22 or P6(5)22, with a = 93.26 A and c = 612.20 A and diffract to 2.9 A resolution at 100 K. Assuming two cross-linked complexes per asymmetric unit yields a reasonable V(M) of 2.97 A3/Da.
Lapko
A
A
Max-Delbruck-Centrum für Molekulare Medizin, Berlin, Germany.
Müller
A
A
Heese
O
O
Ruckpaul
K
K
Heinemann
U
U
D000325:Adrenodoxin / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry*
D003432:Cross-Linking Reagents; D000325:Adrenodoxin; D005287:Ferredoxin-NADP Reductase; D005022:Ethyldimethylaminopropyl Carbodiimide