Up-regulation of the Nedd2 gene encoding an ICE/Ced-3-like cysteine protease in the gerbil brain after transient global ischemia.
We assessed the expression of several genes encoding pro-apoptotic cysteine proteases similar to interleukin-1 beta converting enzyme (ICE) and nematode Ced-3 in association with delayed neuronal death (DND) after transient forebrain ischemia in Mongolian gerbil. The levels of the two species of Nedd2 mRNA concomitantly increased about two-fold in the whole forebrain at 3-6 h after 10-min ischemia and declined to the basal level by 24 h. In situ hybridization revealed that the Nedd2 gene was up-regulated in some neuronal populations in CA1 and CA3 regions of the hippocampus. In contrast, expression of ICE, CPP32/Yama/Apopain, and TX/ICErelll did not change within 48 h. These observations raise the possibility that up-regulation of Nedd2 in the vulnerable neurons may contribute to the proteolytic processes preceding the manifestation of apoptosis and/or necrosis after ischemic insult.
Kinoshita
M
M
Department of Molecular Oncology, Graduate School of Medicine, Kyoto University, Japan.
Tomimoto
H
H
Kinoshita
A
A
Kumar
S
S
Noda
M
M
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D003546:Cysteine Endopeptidases / Q000235:genetics*; D015870:Gene Expression; D005849:Gerbillinae; D017403:In Situ Hybridization; D002546:Ischemic Attack, Transient / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D015854:Up-Regulation
D012333:RNA, Messenger; D003546:Cysteine Endopeptidases