title: Disseminated peritoneal leiomyomatosis. Clonality analysis by X chromosome inactivation and cytogenetics of a clinically benign smooth muscle proliferation.
abstract: Disseminated peritoneal leiomyomatosis (DPL, leiomyomatosis peritonealis disseminata) is a rare condition in which multiple histologically benign smooth muscle tumorlets diffusely stud peritoneal and omental surfaces in females, predominantly of reproductive age. Although the distribution of these lesions suggests a metastatic process, DPL generally has a benign clinical course and has been regarded as a metaplastic process. We assessed clonality of 42 tumorlets and 15 normal tissues from four females with DPL by analyzing X chromosome inactivation as indicated by the methylation status of the androgen receptor gene (HUMARA). In each of the four patients, the same parental X chromosome was nonrandomly inactivated in all tumorlets, consistent with a metastatic unicentric neoplasm, or alternatively, selection for an X-linked allele in clonal multicentric lesions. Anomalous demethylation of the marker for X inactivation (HUMARA) was associated with loss of heterozygosity for markers spanning the X chromosome, or monosomy X, in part of one leiomyomatous lesion. Biallelic demethylation of the HUMARA microsatellite polymorphism was also found in one intramural leiomyoma. Two of six DPL lesions karyotyped had cytogenetic abnormalities involving chromosomes 7, 12, and 18, suggesting a pathogenesis in common with uterine leiomyomas.
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Quade
authors.forename: C M
authors.initials: CM
authors.lastname: McLachlin
authors.forename: V
authors.initials: V
authors.lastname: Soto-Wright
authors.forename: J
authors.initials: J
authors.lastname: Zuckerman
authors.forename: G L
authors.initials: GL
authors.lastname: Mutter
authors.forename: C C
authors.initials: CC
authors.lastname: Morton
mesh_terms: D000328:Adult; D002872:Chromosome Deletion; D019175:DNA Methylation; D004303:Dosage Compensation, Genetic; D005260:Female; D006579:Heterozygote; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D017436:Kallmann Syndrome / Q000235:genetics; D007621:Karyotyping; D018231:Leiomyomatosis / Q000235:genetics* / Q000473:pathology; D018895:Microsatellite Repeats; D010534:Peritoneal Neoplasms / Q000235:genetics* / Q000473:pathology; D011110:Polymorphism, Genetic; D011944:Receptors, Androgen / Q000235:genetics; D014960:X Chromosome / Q000235:genetics
chemical_list: D011944:Receptors, Androgen; D007041:Hypoxanthine Phosphoribosyltransferase