title: The integration of nitrogen and carbon catabolite repression in Aspergillus nidulans requires the GATA factor AreA and an additional positive-acting element, ADA.
abstract: The expression of the structural genes of the proline utilization cluster of Aspergillus nidulans is repressed efficiently only when both repressing carbon and nitrogen sources are present. Two hypotheses can account for this fact. One is a direct or indirect competition mechanism between the positive-acting AreA GATA factor, mediating nitrogen metabolite repression, and the negative-acting CreA protein, mediating carbon catabolite repression. The second is to propose that CreA prevents the binding or activity of another, as yet unidentified, positive-acting factor, here called ADA. We show the second possibility to be the correct one, and we localize the new positive cis-acting element within 290 bp of the prnD-prnB divergent promoter.
authors.affiliation: Institut de Génétique et Microbiologie, Unité de Recherche Associée au CNRS D2225, Université Paris-Sud, Orsay, France. rgonzalez@igmors.u-psud.fr
authors.forename: R
authors.initials: R
authors.lastname: Gonzalez
authors.forename: V
authors.initials: V
authors.lastname: Gavrias
authors.forename: D
authors.initials: D
authors.lastname: Gomez
authors.forename: C
authors.initials: C
authors.lastname: Scazzocchio
authors.forename: B
authors.initials: B
authors.lastname: Cubero
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics*; D001667:Binding, Competitive; D002244:Carbon / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family; D009584:Nitrogen / Q000378:metabolism; D011392:Proline / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014157:Transcription Factors / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: C079728:AreA protein, Aspergillus nidulans; D005656:Fungal Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C071237:CreA protein, Aspergillus nidulans; D002244:Carbon; D011392:Proline; D009584:Nitrogen