Joint cultivation of human erythroblastoid cells and mouse fibroblasts triggers release of a wide spectrum of cytotoxic factors.
It has been demonstrated that joint cultivation of human K-562 cells and mouse L-929 cells results in triggering apoptosis in L-929 cells and possibly also in K-562 cells. Analysis of proteins recovered from the culture medium has revealed the presence of three cytotoxic factors including TNF-alpha. This is the first observation of a release of cytotoxic factors by non-lymphoid cells in response to a direct contact with cells of different origin.
Gnuchev
N V
NV
Institute of Gene Biology RAS, Moscow, Russia.
Sashchenko
L P
LP
Iarovaia
O V
OV
Dukhanina
E
E
Lukianova
T
T
Razin
S V
SV
D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D053938:DNA Fragmentation; D004900:Erythroblasts / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D017077:Culture Media, Conditioned; D014409:Tumor Necrosis Factor-alpha