Glial development in the Drosophila CNS requires concomitant activation of glial and repression of neuronal differentiation genes.
Two classes of glial cells are found in the embryonic Drosophila CNS, midline glial cells and lateral glial cells. Midline glial development is triggered by EGF-receptor signalling, whereas lateral glial development is controlled by the gcm gene. Subsequent glial cell differentiation depends partly on the pointed gene. Here we describe a novel component required for all CNS glia development. The tramtrack gene encodes two zinc-finger proteins, one of which, ttkp69, is expressed in all non-neuronal CNS cells. We show that ttkp69 is downstream of gcm and can repress neuronal differentiation. Double mutant analysis and coexpression experiments indicate that glial cell differentiation may depend on a dual process, requiring the activation of glial differentiation by pointed and the concomitant repression of neuronal development by tramtrack.
Giesen
K
K
Institut für Entwicklungsbiologie, Universität zu Köln, Germany.
Hummel
T
T
Stollewerk
A
A
Harrison
S
S
Travers
A
A
Klämbt
C
C
D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation / Q000235:genetics; D002490:Central Nervous System / Q000196:embryology* / Q000254:growth & development; D004268:DNA-Binding Proteins / Q000502:physiology; D004330:Drosophila / Q000196:embryology* / Q000235:genetics / Q000254:growth & development*; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000502:physiology; D004627:Embryonic Induction; D018507:Gene Expression Regulation, Developmental; D005816:Genetic Complementation Test; D009154:Mutation; D009419:Nerve Tissue Proteins; D009457:Neuroglia / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology; D009479:Neuropeptides / Q000502:physiology; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000502:physiology; D012097:Repressor Proteins; D015534:Trans-Activators / Q000502:physiology; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C102805:GCM protein, Drosophila; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C083884:pnt protein, Drosophila; C075878:ttk protein, Drosophila