Isolation, partial purification, and ultrastructure of taste bud cells from rabbit foliate papillae.
A method is described for obtaining large numbers of isolated taste bud cells from lingual epithelium of rabbit foliate papillae. The isolation method is based on isopyenic sedimentation in a Percoll gradient. The purification of taste bud cells was evaluated by electron microscopy and by immunohistochemistry using CK 20 antibody. The cytology of the isolated taste bud cells remained very similar to in situ cells. The type III cells, which are regarded as gustatory cells, retained their characteristic dense-cored granules in the cytoplasm. This method will permit study of various parameters of taste bud cell biology.
Seta
Y
Y
Department of Oral Anatomy II, Kyushu Dental College, Kokurakita-ku, Kitakyushu, Japan.
Harada
H
H
Toyoshima
K
K
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003102:Colloids; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D011205:Povidone; D011817:Rabbits; D012822:Silicon Dioxide; D013650:Taste Buds / Q000166:cytology* / Q000648:ultrastructure; D014059:Tongue / Q000166:cytology*
D003102:Colloids; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone