title: Keratin intermediate filament dynamics in cell heterokaryons reveals diverse behaviour of different keratins.
abstract: To study the dynamics of keratin intermediate filaments, we fused two different types of epithelial cells (PtK2 and BMGE+H) and studied how the keratins from the parental cells recombine and copolymerize to form the heterokaryon cytoskeleton. The behaviour of the keratins during this process was followed by immunofluorescence using specific antibodies. After fusion, the parental cytoskeletons undergo a depolymerization process most apparent in the region adjacent to the fusion area. The depolymerized subunits spread throughout the heterokaryon and copolymerize into a new hybrid cytoskeleton. The complete process is very rapid, occurring in 3-4 hours, thus demonstrating the highly dynamic nature of the keratin cytoskeleton. Although newly synthesised subunits contribute to the formation of the hybrid cytoskeleton, the process takes place with similar kinetics in the absence of protein synthesis, showing the dynamic nature of the keratins from pre-existing cytoskeletons. During this process, specific keratins behave differently. Keratins K8, K18, K5 and K10 are mobilised from the parental cytoskeletons and reassemble rapidly into the hybrid cytoskeleton (3-6 hours), whereas K14 requires a substantially longer period (9-24 hours). Thus, different keratins, even when they form part of the same heterodimeric/tetrameric complexes, as is the case for K5 and K14, exhibit different dynamics. This suggests that individual polypeptides or homopolymeric complexes rather than exclusively heterodimeric/ tetrameric subunits, as is currently thought, can also take part in keratin intermediate filament assembly and dynamics. Biochemical analysis performed in the absence of protein synthesis revealed greater amounts of K5 than of K14 in the soluble pool of BMGE+H cells. Crosslinking and immunoprecipitation experiments indicated an excess of monomeric K5, as well as of K5/K14 heterodimers and K5 homodimers in the soluble pool. These results are in agreement with the different dynamic behaviour of these keratins observed in immunofluorescence. On the contrary, the phosphorylation levels of K5 and K14 are similar in both the soluble pool and the polymerized fraction, suggesting that phosphorylation does not play an important role in the different dynamics displayed by these two proteins. In summary, our results demonstrate that, following fusion, the keratin intermediate filament network reshapes rather rapidly and that keratins are highly dynamic proteins, although this mobility depends on each particular polypeptide.
authors.affiliation: Department of Cell and Molecular Biology, CIEMAT, Madrid, Spain. chus@ciemat.es
authors.forename: J M
authors.initials: JM
authors.lastname: Paramio
authors.forename: M L
authors.initials: ML
authors.lastname: Casanova
authors.forename: A
authors.initials: A
authors.lastname: Alonso
authors.forename: J L
authors.initials: JL
authors.lastname: Jorcano
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D003599:Cytoskeleton / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006822:Hybrid Cells / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D053550:Keratin-10; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D007614:Macropodidae; D008321:Mammary Glands, Animal / Q000166:cytology; D011092:Polyethylene Glycols; D012995:Solubility
chemical_list: D053550:Keratin-10; D011092:Polyethylene Glycols; D007633:Keratins