Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation.
Three polymerase chain reaction-based assays for the mouse Lepob mutation are described: Dde I site created by the C-->T transversion characterizing Lepob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A-->C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the heterozygote.
Chung
W K
WK
Laboratory of Human Behavior and Metabolism, Rockefeller University, New York, NY 10021, USA.
Chua
S C
SC
Lee
G H
GH
Leibel
R L
RL
D000595:Amino Acid Sequence; D000818:Animals; D004586:Electrophoresis / Q000379:methods; D005838:Genotype; D051379:Mice; D008820:Mice, Obese / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length