Molecular detection and identification of Paracoccidioides brasiliensis.
Nearly 800 nucleotides from the 5' terminus of the 28S ribosomal gene of Paracoccidioides brasiliensis were sequenced, and a 14-base DNA probe specific for this species was identified. Hybridization results showed that the probe identified P. brasiliensis ribosomal DNA in a panel of ribosomal DNAs representing a total of 48 species of fungi.
Sandhu
G S
GS
Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
Aleff
R A
RA
Kline
B C
BC
da Silva Lacaz
C
C
D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010228:Paracoccidioides / Q000145:classification* / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000382:microbiology*
D004271:DNA, Fungal