FT-Raman studies on the transformation of G-actin to F-actin, the binding of cisplatin and transplatin to F-actin and the effects of the conformation of F-actin.
The conformation change of G-actin of F-actin and the binding modes of cisplatin and transplatin to F-actin have been studied by FT-Raman spectroscopy. The studies show that the process of polymerization is related to the vibration of C-S Gauche mode (approximately 650 cm-1), which indicates that the methionine (Met) contributes to the polymerization of actin. The relative intensity of I(925)/I(803), reflecting the conformation of actin secondary structure, does not change during the polymerization process. The effect of cisplatin and transplatin on F-actin is dependent on the species and their concentrations. Cisplatin, at high concentrations, affects the conformation of F-actin mainly by binding with the sulphur of methionine. Transplatin, even at low concentrations, obviously affects the F-actin's conformation due to it's multiple binding sites, on N-containing sites in addition to S-methionine sites. These results relate to the differences in pharmacology and toxicology effects of the complexes.
Zeng
H H
HH
School of Pharmaceutical Sciences, Beijing Medical University, China.
Xu
Z H
ZH
Wang
K
K
D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D001665:Binding Sites; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D002945:Cisplatin / Q000378:metabolism*; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008715:Methionine / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
D000199:Actins; D000970:Antineoplastic Agents; D001704:Biopolymers; D046911:Macromolecular Substances; C034697:transplatin; D000255:Adenosine Triphosphate; D008715:Methionine; D002945:Cisplatin