title: Bacterial expression and characterization of an anti-desipramine single-chain antibody fragment.
abstract: Tricyclic antidepressant toxicity is a leading cause of death from intentional drug overdose. Monoclonal antibody Fab' fragments specific for the tricyclic antidepressant, desipramine, reverse acute drug toxicity but may themselves have adverse effects at therapeutic doses. To evaluate the characteristics of smaller antibody fragments, we cloned, expressed and characterized a 26 kD single chain Fv fragment (G5-sFv). A DNA sequence encoding VH-linker-V1 was constructed from hybridoma mRNA encoding a high affinity monoclonal desipramine specific IgG1 and expressed in E. coli. G5-sFv was produced at high levels as insoluble inclusion bodies. Single chain Fv was solubilized, folded in a redox buffer and affinity purified on desipramine-Sepharose. The affinity of G5-sFv for desipramine was similar to that of the corresponding monoclonal Fab' as measured by surface plasmon resonance (Fab' 5.5 +/- 0.5 x 10(8) M-1, sFv 2.3 +/- 0.5 x 10(8) M-1). G5-sFv administered to rats after a tracer dose of 3H-desipramine produced rapid and marked redistribution of drug from tissues into serum. G5-sFv was stable at 4 C for greater than 6 months but lost activity at higher temperatures. We conclude that desipramine-specific-single chain Fv expressed in E. coli retains the affinity of the parent antibody for desipramine. The pharmacokinetic effect of G5-sFv on desipramine distribution suggests that it may be useful as an antidote for desipramine overdose.
authors.affiliation: Department of Veterinary PathoBiology, University of Minnesota, St Paul 55108, USA.
authors.forename: G
authors.initials: G
authors.lastname: Lin
authors.forename: P R
authors.initials: PR
authors.lastname: Pentel
authors.forename: W L
authors.initials: WL
authors.lastname: Shelver
authors.forename: D E
authors.initials: DE
authors.lastname: Keyler
authors.forename: C A
authors.initials: CA
authors.lastname: Ross
authors.forename: Y
authors.initials: Y
authors.lastname: Hieda
authors.forename: M C
authors.initials: MC
authors.lastname: Flickinger
authors.forename: C A
authors.initials: CA
authors.lastname: Pennell
authors.forename: M P
authors.initials: MP
authors.lastname: Murtaugh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry; D000929:Antidepressive Agents, Tricyclic / Q000276:immunology*; D001483:Base Sequence; D002645:Chickens; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D003891:Desipramine / Q000276:immunology*; D062787:Drug Overdose / Q000628:therapy; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D007128:Immunoglobulin Fragments / Q000737:chemistry; D008969:Molecular Sequence Data; D011506:Proteins / Q000302:isolation & purification; D051381:Rats
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000929:Antidepressive Agents, Tricyclic; D007128:Immunoglobulin Fragments; D011506:Proteins; D003891:Desipramine