Methylmercury modulation of monocyte chemotactic protein-1 mRNA expression in human peripheral blood mononuclear cells.
The effect of methylmercury (MeHg; CH3HgCl) on the gene expression of monocyte chemotactic protein-1 (MCP-1) by human peripheral blood mononuclear cells (PBMC) was examined. PBMC were exposed with or without thrombin (1 U/ml) or MeHg (0.3 or 3.0 microM) for 24 hours. The total RNA was reverse transcribed and then amplified by the method of reverse transcriptase-polymerase chain reaction (RT-PCR). Thrombin enhanced MCP-1 mRNA expression in PBMC. MeHg inhibited thrombin-stimulated MCP-1 mRNA expression in a dose dependent manner. These findings suggest that MeHg affects the atherosclerotic process by changing MCP-1 mRNA expression in PBMC.
Kishimoto
T
T
Department of Environmental Medicine I, Shimane Medical University.
Oguri
T
T
Ueda
D
D
Tada
M
M
D018932:Chemokine CCL2 / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008767:Methylmercury Compounds / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis*
D018932:Chemokine CCL2; D008767:Methylmercury Compounds; D012333:RNA, Messenger