Concanavalin A directly stimulates bone-resorbing activity of osteoclasts and their gene expression of cathepsin K/OC-2.
Concanavalin A (Con A), a plant lectin that recognizes cell-surface glycoproteins, up-regulates osteoclastic bone-resorbing activity of cultured isolated rabbit pure osteoclasts as well as unfractionated bone cells. The effect of Con A was blocked by alpha-methyl mannopyranoside. Several Con A-binding proteins were detected in the plasma membranes from osteoclasts. Furthermore, Con A increased the levels of transcripts of cathepsin K/OC-2, one of the proteases responsible for osteoclastic bone-resorbing activity. These results indicate that Con A directly enhances the function of osteoclasts by the association with surface glycoproteins of osteoclasts.
Kakudo
S
S
Discovery Research Laboratories II, Shionogi & Co., Ltd., Fukushima-ku, Osaka, Japan.
Mano
H
H
Shiokawa
M
M
Mori
Y
Y
Kumegawa
M
M
Hakeda
Y
Y
D000818:Animals; D001862:Bone Resorption; D056657:Cathepsin K; D002403:Cathepsins / Q000235:genetics*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D010010:Osteoclasts / Q000187:drug effects*; D011817:Rabbits
D003208:Concanavalin A; D002403:Cathepsins; D056657:Cathepsin K