Experimental gluing of lung parenchyma in rats.
Gelatin-resorcinol-dialdehyde adhesive has been developed from a gelatin-resorcinol-formaldehyde adhesive by replacing the formaldehyde with two less histotoxic dialdehydes, ethandial and pentandial. The aim of the present study was to evaluate the usefulness of this modified composition in gluing defects in lung parenchyma. In 40 male Wistar rats a standardized lung incision 1.0 cm in length and 0.8 cm in depth were closed by application of gelatin-resorcinol-dialdehyde adhesive. For macroscopic and microscopic examination 4 animals were sacrificed on each of postoperative days 2, 7, and 14 and 14 animals on each of postoperative days 28 and 120. Macroscopic examination revealed a tight closure of the parenchymal defects in all postoperative stages. Initially by an adhesive layer and later on by granulation tissue and scar tissue respectively. On microscopic examination an inflammatory tissue response with polymorphonuclear neutrophils and macrophages predominating was found 2 days postoperatively. After 7 days multinucleated giant cells appeared. On postoperative day 14 the tissue response presented a distinct granulomatous character with multinucleated giant cells persisting. After 28 days remnants of adhesive surrounded by granulation tissue were detectable. On postoperative day 120 the adhesive had been completely resorbed and the parenchymal defect was replaced by fibrous scar tissue. The gelatin-resorcinol-adhesive proved effective in tight closure of lung parenchyma in rats. The adhesive is resorbed completely and does not interfere with parenchymal healing.
Wertzel
H
H
Department of Thoracic Surgery, University of Freiburg, Germany.
Wagner
B
B
Stricker
A
A
Swoboda
L
L
Hasse
J
J
Lange
W
W
Freudenberg
N
N
D000042:Absorption; D000818:Animals; D002921:Cicatrix / Q000473:pathology; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D005780:Gelatin / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D005976:Glutaral / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D006037:Glyoxal / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D008168:Lung / Q000601:surgery*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012118:Resorcinols / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D013997:Time Factors; D014014:Tissue Adhesives / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D014945:Wound Healing
D004338:Drug Combinations; D012118:Resorcinols; D014014:Tissue Adhesives; C087754:gelatin-resorcinol-pentanedial-ethanedial adhesive; D006037:Glyoxal; D005780:Gelatin; D005976:Glutaral