The resistance and integrase genes of pACM1, a conjugative multiple-resistance plasmid, from Klebsiella oxytoca.
pACM1 is an 85-kb conjugative plasmid from a clinical isolate of Klebsiella oxytoca that encodes resistance to beta-lactams (mediated by SHV-5 extended spectrum beta-lactamase), trimethoprim, sulfonamides, tetracycline, aminoglycosides, and mercuric chloride. The expression of the aminoglycoside resistance is difficult to detect, which could have clinical implications. A region of pACM1 containing five resistance genes and two putative integrons was characterized by restriction mapping and partial DNA sequencing. One integron appears to be class I (sull type); the second lacks a recognizable 3' conserved segment. Neither integron has the BamHI site predicted for the 5' conserved segment. Plasmids encoding SHV-5 from other bacterial strains appear to be closely related to pACM1 by restriction enzyme analysis, but have resistance/ integron regions that vary in size and content from that of pACM1. Integrase-mediated recombination might be responsible for genetic divergence in a widely distributed family of pACM1-like plasmids.
Preston
K E
KE
Department of Pathology and Laboratory Medicine, Albany Medical College, New York 12208, USA.
Kacica
M A
MA
Limberger
R J
RJ
Archinal
W A
WA
Venezia
R A
RA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D005798:Genes, Bacterial; D019450:Genes, MDR; D006801:Humans; D019426:Integrases / Q000235:genetics*; D007709:Klebsiella / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011815:R Factors / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D019426:Integrases