Molecular cloning of a new unc-33-like cDNA from rat brain and its relation to paraneoplastic neurological syndromes.
Anti-CV2-autoantibodies from patients with paraneoplastic neurological syndromes were used to purify protein(s) related to this disease. A novel cDNA, c-22, was obtained by PCR with primers based on amino-acid sequence of peptides obtained from this protein and rat brain cDNA as template. The deduced amino-acid sequence of c-22 shows homology to the Unc-33 gene from C. elegans in which mutations lead to defects in neuritic outgrowth and axonal guidance and cause uncoordinated movements of the nematode. Several consensus sites for putative protein kinase C phosphorylation were found, suggesting that the c-22 gene product may be a phosphoprotein. Northern hybridizations show that the apparently unique 3.8-kb mRNA of c-22 is present in rat brain tissue and its expression is developmentally regulated: the levels of C-22 mRNA, detectable in brain at embryonic day 17 (E17), increase up to post-natal day 7 (P7) and decline rapidly to an almost undetectable level in adult.
Quach
T T
TT
Faculté de Medécine Laennec, INSERM (U433), Lyon, France.
Rong
Y
Y
Belin
M F
MF
Duchemin
A M
AM
Akaoka
H
H
Ding
S
S
Baudry
M
M
Kolattukudy
P E
PE
Honnorat
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001932:Brain Neoplasms / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D010257:Paraneoplastic Syndromes / Q000235:genetics*; D051381:Rats
D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D015801:Helminth Proteins; D009414:Nerve Growth Factors; C078652:unc-33 protein, C elegans