title: A 4-term energy level scheme for the high-spin ferrous hemoproteins: evidence for the 5E eta, and 5B2 terms as the ground multiplets in hemoproteins with a histidine and a cysteine protein-derived heme ligand, respectively.
abstract: We have carried out analysis of the electronic level scheme of the high-spin ferrous hemoproteins by simultaneous fit of the adjustable parameters of a 4-term theoretical model to low-temperature magnetic circular dichroism (MCD), room temperature absorption spectra and available magnetic susceptibility and or Mössbauer data of myoglobin, horseradish peroxidase and cytochrome P450. The high reliability of the ligand field parameter values obtained for deoxymyoglobin is confirmed by good agreement between the predicted and observed magnetic field dependences of MCD and magnetization not used in the fit procedure. In addition, an energy gap between the ground and first excited singlets, estimated to be 4.2 cm-1, agrees well with the value of approximately 4 cm-1 derived from the far-infrared magnetic resonance. Our computer and explicit theoretical analyses give strong evidence that large distinctions in the shape, intensity and temperature behaviour of the MCD of Mb and HRP from those of cytochrome P450 can be described only if the ground manifold in these proteins is 5E eta and 5B2, respectively. The changes in relative energies of the one-electron 3d-orbitals on substitution of an imidazole of histidine for a sulphur anion of cysteine as a protein-derived heme iron ligand are rationalized by the lower ionization potential of the negatively charged sulphur ligand and the higher pi-orbital overlap of its lone pair orbitals with the iron d pi-orbitals compared to the imidazole ligand.
authors.affiliation: Engelhardt Institute of Molecular Biology, Academy of Sciences of Russia, Moscow, Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Oganesyan
authors.forename: Y A
authors.initials: YA
authors.lastname: Sharonov
mesh_terms: D000818:Animals; D002711:Chloride Peroxidase / Q000737:chemistry; D002942:Circular Dichroism; D003198:Computer Simulation; D003545:Cysteine / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D006418:Heme / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009211:Myoglobin / Q000031:analogs & derivatives* / Q000737:chemistry; D015204:Spectroscopy, Mossbauer; D013696:Temperature; D013816:Thermodynamics
chemical_list: D007093:Imidazoles; D009211:Myoglobin; C055547:deoxymyoglobin; D006418:Heme; D006639:Histidine; D003577:Cytochrome P-450 Enzyme System; D006735:Horseradish Peroxidase; D002711:Chloride Peroxidase; D003545:Cysteine