Enhancement of humoral immunity to SIVenv following simultaneous inoculation of mice by three recombinant adenoviruses encoding SIVenv/poliovirus chimeras, Tat and Rev.
A means of inducing gene expression by simultaneous infection with three recombinant adenoviruses (Ad) is described. The simian immunodeficiency virus (SIV) envelope-coding region was placed under the control of the human immunodeficiency virus type 1 (HIV-1) Tat and Rev proteins provided in trans by distinct Ad vectors (Ad-tat; Ad-rev). Coinfection of cells with the three recombinant adenoviruses led to induction of high levels of SIV env mRNA and protein synthesis, while inoculation of mice elicited anti-Env antibodies. Insertion of the poliovirus VP1 neutralization epitope (C3) in the V1 hypervariable region of SIV envelope not only proved to be highly immunogenic for the poliovirus epitope but also enhanced the kinetics of anti-SIV Env antibody production. By contrast, insertion in V4 elicited no anti-C3 response and only normal anti-Env responses.
Chenciner
N
N
Unite de Retrovirologie Moleculaire, Institut Pasteur, Paris, France.
Randrianarison-Jewtoukoff
V
V
Delpeyroux
F
F
Hanania
N
N
Pedroza Martins
L
L
Stratford Perricaudet
L
L
Perricaudet
M
M
Wain-Hobson
S
S
D000256:Adenoviridae; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D017931:DNA Primers; D015686:Gene Products, env / Q000276:immunology*; D015694:Gene Products, rev / Q000276:immunology*; D015696:Gene Products, tat / Q000276:immunology*; D014644:Genetic Variation; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins; D015302:Simian Immunodeficiency Virus / Q000201:enzymology* / Q000235:genetics; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
D017931:DNA Primers; D015686:Gene Products, env; D015694:Gene Products, rev; D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus