Quantification of 1,5-anhydro-D-glucitol in urine by automated borate complex anion-exchange chromatography with an immobilized enzyme reactor.
HPLC using a borate form of a strongly anion-exchange resin column and an immobilized enzyme reactor for colorimetric detection was used to quantify urinary 1,5-anhydro-D-glucitol. Urine samples were introduced into the system every 7 min without any pretreatment, and after separation of interfering substances in the column, 1,5-anhydro-D-glucitol was successively detected. Quantitative determination of urinary 1,5-anhydro-D-glucitol was possible within the 1.2-300 micromol/l range. The coefficient of variance was less than 3% and the correlation between results obtained with our system (y) and those obtained by gas chromatography-mass spectrometry (x) was y=0.983x-1.287 micromol/l (n=42, r=0.998).
Tanabe
T
T
Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co., Takasaki-shi, Gunma, Japan.
Tajima
S
S
Suzuki
T
T
Okawa
E
E
Machida
R
R
Ichimura
S
S
Yabuuchi
M
M
D001881:Borates; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D002852:Chromatography, Ion Exchange / Q000295:instrumentation; D003847:Deoxyglucose / Q000652:urine*; D004800:Enzymes, Immobilized; D006801:Humans; D013058:Mass Spectrometry
D001881:Borates; D004800:Enzymes, Immobilized; C006584:1,5-anhydroglucitol; D003847:Deoxyglucose