The stabilizing residues and the functional domains in the hyperthermophilic V-ATPase of Desulfurococcus.
To clarify a universal mechanism of the intramolecular rotation of ATP-synthase, an operon encoding a stable, ancestral ATPase was cloned from a heterotrophic archaeum Desulfurococcus strain SY. The operon of about 7 kbp contained genes E, C, G, A, B and D encoding subunits with predicted molecular weights of 23,217, 41,659, 11,499, 65,476, 52,295, and 24,897, respectively. The sequence was compared with that of Na-ATPase of Enterococcus hirae, A-ATPase of Halobacterium salinarium, V-ATPase of Methanosarcina mazei, and ATP synthase of Methanococcus jannaschii, which are homologous. (1) The cause of hyperthermostability: The main exchanges in the amino acid residues of hyperthermophilic proteins included Asp --> Glu (11 residues of A subunit of E.h.) and, Ser --> Ala. (2) The domains needed for the intramolecular rotation: The domains similar to those established in F-type ATPases were also found in the V-type ATPases of species with a different energy metabolism.
Shibui
H
H
Department of Biochemistry, Jichi Medical School, Tochigi-ken, Japan.
Hamamoto
T
T
Yohda
M
M
Kagawa
Y
Y
D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D001105:Archaea / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D027682:Cation Transport Proteins; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016983:Enterococcus / Q000201:enzymology / Q000235:genetics; D004795:Enzyme Stability; D005798:Genes, Bacterial; D017017:Methanococcus / Q000201:enzymology / Q000235:genetics; D017020:Methanosarcina / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009876:Operon; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D025262:Vacuolar Proton-Translocating ATPases
D027682:Cation Transport Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D000251:Adenosine Triphosphatases; D025262:Vacuolar Proton-Translocating ATPases; C021838:sodium-translocating ATPase; D006180:Proton-Translocating ATPases