Analysis of interacting biopolymer systems by analytical ultracentrifugation.
Many of the functions of biological macromolecules are based on specific interactions. Extended concentration dependent studies of sedimentation coefficients or molecular masses of biopolymers are highly useful for describing the different kinds of association phenomena. These studies allow one to determine the partial concentrations of monomers and associates or reactants and complexes in self-associating systems or heterologous associations, respectively. Furthermore, in combination with corresponding measurements of biological activity these data allow one to estimate the individual activity parameters of components involved in equilibrium processes. The study of self-association and heterologous association using analytical ultracentrifugation, some recent developments therein, and its application to different examples are outlined here.
Behlke
J
J
Max Delbrück Center for Molecular Medicine, Berlin, Germany. behlke@mdc-berlin.de
Ristau
O
O
D001704:Biopolymers / Q000737:chemistry* / Q000302:isolation & purification; D002256:Carbonic Anhydrases / Q000737:chemistry; D003605:D-Amino-Acid Oxidase / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004798:Enzymes / Q000737:chemistry*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010449:Peptide Mapping / Q000379:methods; D010732:Phosphofructokinase-1 / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014461:Ultracentrifugation / Q000379:methods*
D001704:Biopolymers; D004798:Enzymes; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011506:Proteins; D004247:DNA; D003605:D-Amino-Acid Oxidase; D010732:Phosphofructokinase-1; D002256:Carbonic Anhydrases