[Immunohistological study of the nasal mucosa with reference to gamma delta-T cells].
The distribution of gamma delta-T cells (gamma delta-TCR positive cells) in nasal mucosa and polyps was studied in patients with nasal allergy and non-allergic patients. The biopsy specimens from the inferior turbinate and resected polyps were frozen at -70 degrees C and sliced at a thickness of 4 microns with a cryostat. Monoclonal antibodies (CD3 and TCR-gamma delta-1) and the Labelled Streptavidin Biotin method were used to detect T lymphocytes and gamma delta-T cells. The results were as follows: 1) The rate of gamma delta-T cells in the epithelium is higher than that in the lamina propria in patients with nasal allergy. 2) In non-allergic patients, on the other hand, the rate of gamma delta-T cells was almost the same in these layers. 3) The distribution of gamma delta-T cells in nasal polyps was uniform and their rate was relatively high. It has been reported that gamma delta-T cells can recognize a stress antigen such as heat shock protein. These cells are thought to play an important role in non-specific immunoreactions. This study suggests that gamma delta-T cells in the nasal mucosa play an important role also in specific immunoreactions.
Tsurumoto
H
H
Department of Otolaryngology, Nagasaki University School of Medicine.
Takamura
H
H
Takasaki
K
K
Yoshimi
R
R
Kobayashi
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology*; D009298:Nasal Polyps / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D016692:Receptors, Antigen, T-Cell, gamma-delta