[Metabolites produced by Streptomyces kanamyceticus mutants with impaired biosynthesis of kanamycin].
Metabolites produced by Streptomyces kanamyceticus mutants with impaired kanamycin biosynthesis (kan mutants) were investigated by thin layer chromatography. With spectrophotometric scanning of the chromatograms the quantitative content of kanamycin A and 2-desoxystreptamine (2-DOS) in the culture fluid was determined. Five groups of the S.kanamyceticus mutants with impaired kanamycin biosynthesis at various stages were identified: kanA produced no D-glucosamine, kanB and kanC produced no 2-DOS, kanD was not able to transfer 2-DOS to the metabolites with the antibiotic activity, kanG synthesized no kanosamine.
Golets
L M
LM
Esipov
S E
SE
Fedorenko
V A
VA
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002855:Chromatography, Thin Layer; D007612:Kanamycin / Q000096:biosynthesis*; D009154:Mutation; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D007612:Kanamycin