title: Increased endothelial cell retraction and tumor cell invasion by soluble factors derived from pancreatic cancer cells.
abstract: BACKGROUND
  Tumor cells induce endothelial cell retraction before invasion. In pancreatic cancer cells, the factors affecting endothelial cell retraction are not well-understood.
  
  
  METHODS
  The activities of the endothelial cell retraction in conditioned media (CM) derived from three human pancreatic cancer cell lines, PSN-1, MiaPaca-2, and Capan-1, were measured for the amount of intercellular junctional transport of FITC dextran through an endothelial cell monolayer in a transwell cell culture system.
  
  
  RESULTS
  The CM derived from the three pancreatic cancer cells induced endothelial cell retraction. The endothelial cell retraction activity in the CM from PSN-1 cells was significantly higher than those from MiaPaca-2 and Capan-1 cells. The CM from PSN-1 cells enhanced both the adhesion and the invasion of MiaPaca-2 and Capan-1 cells. The factors with endothelial cell retraction activity in the CM from PSN-1 cells were characterized as heat-stable, trypsin-sensitive glycoproteins ranging from 10,000 to 50,000 in molecular weight, and were found both in heparin-bound and unbound fractions.
  
  
  CONCLUSIONS
  PSN-1 cells produced and secreted at least two factors inducing the endothelial cell retraction. The factors could play an important role in the establishment of invasion and metastasis of PSN-1 cells.
authors.affiliation: Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nakamori
authors.forename: H
authors.initials: H
authors.lastname: Okamoto
authors.forename: T
authors.initials: T
authors.lastname: Kusama
authors.forename: K
authors.initials: K
authors.lastname: Shinkai
authors.forename: M
authors.initials: M
authors.lastname: Mukai
authors.forename: H
authors.initials: H
authors.lastname: Ohigashi
authors.forename: O
authors.initials: O
authors.lastname: Ishikawa
authors.forename: H
authors.initials: H
authors.lastname: Furukawa
authors.forename: S
authors.initials: S
authors.lastname: Imaoka
authors.forename: H
authors.initials: H
authors.lastname: Akedo
mesh_terms: D002448:Cell Adhesion; D005591:Chemical Fractionation; D017077:Culture Media, Conditioned; D004727:Endothelium / Q000378:metabolism / Q000473:pathology; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D008970:Molecular Weight; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D017077:Culture Media, Conditioned; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate