title: The C1q-binding cell membrane proteins cC1q-R and gC1q-R are released from activated cells: subcellular distribution and immunochemical characterization.
abstract: Two types of widely coexpressed cell surface C1q-binding proteins (C1q-R): a 60-kDa calreticulin-homolog which binds to the collagen-like "stalk" of C1q and a 33-kDa protein with affinity for the globular "heads" of the molecule, have been described. In this report, we show that the two molecules are also secreted by Raji cells and peripheral blood lymphocytes and can be isolated in soluble form from serum-free culture supernatant by HPLC purification using a Mono-Q column. The two purified soluble proteins had immunochemical and physical characteristics similar to their membrane counterparts in that both bound to intact C1q and to their respective C1q ligands, cC1q and gC1q. In addition, N-terminal amino acid sequence analyses of the soluble cC1q-R and gC1q-R were found to be identical to the reported sequences of the respective membrane-isolated proteins. Ligand blot analyses using biotinylated membrane or soluble cC1q-R and gC1q-R showed that both bind to the denatured and nondenatured A-chain and moderately to the C-chain of C1q. Moreover, like their membrane counterparts, the soluble proteins were found to inhibit serum C1q hemolytic activity. Although cC1q-R was released when both peripheral blood lymphocytes and Raji cells were incubated in phosphate-buffered saline for 1 hr under tissue culture conditions, gC1q-R was releasable only from Raji cells, suggesting that perhaps activation or transformation leading to immortalization is required for gC1q-R release. Subcellular fractionation of Raji cells and analyses by enzyme-linked immunosorbent assay and Western blotting showed that the two molecules are present in the cytosolic fractions as well as on the membrane. The data suggest that soluble forms of both C1q-binding molecules are released from cells and that these molecules may play important roles in vivo as regulators of complement activation.
authors.affiliation: Department of Medicine, State University of New York, Stony Brook 11794-8161, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Peterson
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: P D
authors.initials: PD
authors.lastname: Lu
authors.forename: S A
authors.initials: SA
authors.lastname: Keilbaugh
authors.forename: E I
authors.initials: EI
authors.lastname: Peerschke
authors.forename: B
authors.initials: B
authors.lastname: Ghebrehiwet
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D002051:Burkitt Lymphoma / Q000276:immunology / Q000378:metabolism; D002352:Carrier Proteins; D003166:Complement Activating Enzymes / Q000378:metabolism; D015922:Complement C1q / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D006461:Hemolysis / Q000276:immunology; D006801:Humans; D018960:Hyaluronan Receptors; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000276:immunology; D011951:Receptors, Complement / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C087299:C1QBP protein, human; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D011951:Receptors, Complement; C032402:complement 1q receptor; D015922:Complement C1q; D003166:Complement Activating Enzymes