Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells.
The electrophysiological effects of both trypsinactivated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patchclamp technique. Rates of reduction in whole-cell membrane resistance were correlated with increasing native toxin concentration. The 42- or 51-kDa cloned toxin alone at 50 micrograms/ml reduced the resistance. Electrophysiological effects occurred before any changes were visible by phase-contrast microscopy.
Cokmus
C
C
Department of Biology, Ankara University, Turkey.
Davidson
E W
EW
Cooper
K
K
D000818:Animals; D001407:Bacillus; D001427:Bacterial Toxins / Q000633:toxicity*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003465:Culex / Q000187:drug effects*; D004594:Electrophysiology; D007306:Insecticides / Q000633:toxicity*; D018408:Patch-Clamp Techniques
D001427:Bacterial Toxins; D007306:Insecticides; C046134:binB protein, Bacillus sphaericus