title: Demonstration that the BchH protein of Rhodobacter capsulatus activates S-adenosyl-L-methionine:magnesium protoporphyrin IX methyltransferase.
abstract: The bchH gene of Rhodobacter capsulatus has been cloned into an expression strain of Escherichia coli. Following induction of expression of the BchH protein, it was found that the E. coli strain also accumulated porphyrins with the fluorescence properties of protoporphyrin and zinc protoporphyrin. It was also found that the soluble BchH protein increased the activity of S-adenosyl-L-methionine:magnesium protoporphyrin IX methyltransferase, when mixed with membranes of an expression strain of E. coli into which the bchM gene (which encodes the methyltransferase) had been cloned, as well as membranes of a bchH mutant of R. capsulatus.
authors.affiliation: Department of Chemistry and Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, B.C., Canada.
authors.forename: S B
authors.initials: SB
authors.lastname: Hinchigeri
authors.forename: B
authors.initials: B
authors.lastname: Hundle
authors.forename: W R
authors.initials: WR
authors.lastname: Richards
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism*; D008969:Molecular Sequence Data; D011524:Protoporphyrins / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D011524:Protoporphyrins; D011994:Recombinant Proteins; C033434:protoporphyrin IX monomethyl ester; D008780:Methyltransferases; C060234:magnesium-protoporphyrin methyltransferase