[Establishment of a new human ovarian serous cystadenocarcinoma cell line (IM), and influence on cell proliferations by cisplatin with or without hyperthermia].
A new cell line, designated IM has been established from operation material derived from a woman with ovarian serous cystadenocarcinoma. The population doubling time of the 35th passage IM cell was 28.8 hours. And it was successively subcultured 165 times in over 7 years, moreover still kept CA125 production. The nuclear DNA and cell surface CA125 antigen were double stained by propidium iodide and anti CA125 monoclonal antibody-FITC. Then the two color cytogram obtained by flow cytometry was drawn up. For the most part of CA125 positive cell retained G0 + G1 of cell cycle, the lesser part was in G2 + M phase. The S phase rate of IM cell incubated with cisplatin at 37 degrees C or 41 degrees C for 1 hour that estimated by BrdU-propidium iodide double stain method of flow cytometry, it was suggested that the inhibition of DNA synthesis by cisplatin was increased with 41 degrees C low hyperthermia.
Iida
T
T
Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Yokohama City Seibu Hospital, Japan.
Yoshida
N
N
Kuramoto
H
H
Shimoda
T
T
Hamano
M
M
Hata
H
H
Yonamine
K
K
Hayashi
K
K
D018394:CA-125 Antigen / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology*; D018284:Cystadenocarcinoma, Serous / Q000473:pathology*; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D018394:CA-125 Antigen; D002945:Cisplatin