Induction of plasma hepatocyte growth factor in acute colitis of mice.
OBJECTIVE
The study examined plasma levels of hepatocyte growth factor (HGF) in acute colitis models on mice.


METHODS
Acute colitis was induced in male FVB mice (about 25 g) by intrarectal administration of 200 microliters of 0.5% acetic acid.


METHODS
Plasma was taken at 0, 12, 24, 36, 48 and 72 h after acetic acid treatment. Plasma HGF was measured by an enzyme immunoassay in duplicate.


RESULTS
Plasma levels of HGF at 0, 12, 24, 36, 48 and 72 h after acetic acid administration were 0.31 +/- 0.11 ng/ml (n = 4), 0.45 +/- 0.04 ng/ml (n = 4), 0.46 +/- 0.13 ng/ml (n = 4), 0.71 +/- 0.22 ng/ml (n = 4), 0.82 +/- 0.15 ng/ml (n = 4) and 0.44 +/- 0.04 ng/ml (n = 4), respectively. Significant increases in plasma HGF levels were observed at 36 and 48 h after the treatment (p < 0.05, compared to at 0 h).


CONCLUSIONS
Plasma HGF was induced by acute inflammation of colonic tissues. These findings suggest that HGF in blood may be one of the non-specific markers of inflammation in mice.
Matsuno
M
M
Second Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.
Shiota
G
G
Umeki
K
K
Kawasaki
H
H
Kojo
H
H
Miura
K
K
D019342:Acetic Acid / Q000008:administration & dosage / Q000633:toxicity; D000208:Acute Disease; D000285:Administration, Rectal; D000818:Animals; D015415:Biomarkers / Q000097:blood; D003092:Colitis / Q000097:blood* / Q000139:chemically induced; D004195:Disease Models, Animal; D017228:Hepatocyte Growth Factor / Q000097:blood*; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents / Q000008:administration & dosage / Q000633:toxicity; D007249:Inflammation / Q000097:blood; D008297:Male; D051379:Mice
D015415:Biomarkers; D007202:Indicators and Reagents; D017228:Hepatocyte Growth Factor; D019342:Acetic Acid