Calbindin-D28k immunoreactivity in the rat amygdala.
Calbindin-D28k (CB) is a calcium-binding protein whose exact function has yet to be elucidated. Because CB is contained in distinct cell types in the nervous system, it is a valuable marker for distinguishing specific nuclear subdivisions and neuronal populations. In the present study, immunohistochemical methods were used to localize CB in the rat amygdala. A subpopulation of nonpyramidal neurons in all nuclei of the basolateral amygdala (ABL) exhibited intense CB immunoreactivity (CB-ir). CB-positive puncta resembling axon terminals were observed surrounding pyramidal perikarya in the ABL. Pyramidal neurons in caudal and lateral portions of the ABL exhibited moderate CB-ir. Intensely stained nonpyramidal neurons resembling those of the ABL were also seen in the cortical nuclei, periamygdaloid cortex, and nucleus of the lateral olfactory tract; these nuclei also contained variable numbers of moderately stained pyramidal cells. Numerous CB-positive neurons were observed in all subdivisions of the medial nucleus. The posterodorsal subdivision of the medial nucleus exhibited a centrally located island of small CB-negative neurons and three cell-dense clusters of CB-positive neurons. The distribution of CB-ir in the central nuclear complex was very heterogeneous. The intermediate subdivision of the central nuclear complex exhibited the most robust staining, whereas the lateral subdivision contained relatively few CB-positive cells. Dorsal and ventral portions of the lateral capsular subdivision of the central nuclear complex could be readily distinguished on the basis of differing levels of CB-ir. These results indicate that CB is localized in discrete cell types and nuclear subdivisions in the rat amygdala and suggest that CB immunohistochemistry is a useful technique for identifying specific structural components in this brain region.
McDonald
A J
AJ
Department of Cell Biology and Neuroscience, University of South Carolina School of Medicine, Columbia 29208, USA. McDonald@dcsmserver.med.sc.edu
D000679:Amygdala / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D007150:Immunohistochemistry; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017966:Pyramidal Cells / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G