title: Kinetic analysis of taurine influx into cerebral cortical slices from adult and developing mice in different incubation conditions.
abstract: The influx of taurine into cerebral cortical slices was studied with 3-day-old and 3-month-old mice in different ionic environments in incubation medium. In standard Krebs-Ringer medium the influx comprised two saturable uptake components, high- and low-affinity, and non-saturable penetration. In isoosmotic medium potassium stimulation abolished the high-affinity uptake in both age groups. In hyperosmotic medium the high-affinity uptake disappeared totally in 3-day-old mice and partially in 3-month-old mice. The high-affinity uptake was also obliterated in hypoosmotic medium and in the absence of chloride ions in both age groups. The low-affinity uptake was abolished by potassium stimulation in 3-month-olds and strongly inhibited in 3-day-olds. Hypoosmotic and chloride-free media also inhibited the low-affinity uptake at both ages. Non-saturable influx was greatly diminished in chloride-free media. The taurine uptake systems are thus strongly inhibited in incubation conditions which simultaneously evoke apparent release of taurine from cerebral cortical slices. This inhibition contributes to the magnitude of the estimated release, which in vitro represents overflow of released taurine molecules which escape recapture by the membrane carriers. In vivo the same mechanism may underlie the delayed and spreading neuromodulatory actions of taurine.
authors.affiliation: Department of Physiology, Tampere Brain Research Center, University of Tampere Medical School, Tampere, Finland.
authors.forename: S S
authors.initials: SS
authors.lastname: Oja
authors.forename: P
authors.initials: P
authors.lastname: Saransaari
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002712:Chlorides / Q000502:physiology; D003470:Culture Media; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009994:Osmolar Concentration; D011188:Potassium / Q000494:pharmacology*; D012044:Regression Analysis; D013654:Taurine / Q000378:metabolism*
chemical_list: D002712:Chlorides; D003470:Culture Media; D013654:Taurine; D011188:Potassium