[Splenic lymphoma with villous lymphocytes: morphologic, immunologic and molecular study. Report of three cases].
Splenic lymphoma with villous lymphocytes (SLVL) is a low grade lymphoproliferation characterized by a massive splenomegaly, an absence of lymphadenopathy and the presence in the peripheral blood of atypical B-lymphocytes with hairy-cell appearance. We have studied the morphological, immunological and molecular characteristics of 3 cases of SLVL. SLVL presented on blood smears characteristic irregularities of the plasma membrane consisting in thin and short villi unevenly distributed. The main phenotype was CD5-, CD11c+, and CD25-, but individual SLVL cases can not be identified by using immunohistochemical criteria alone. Clonal rearrangements of the immunoglobulin heavy chain gene were found in all 3 cases and in one case presented a bcl2-JH rearrangement. SLVL are clonal B-cell lymphoproliferations and can be associated with t(14; 18) translocation.
Labouyrie
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Laboratoire d'Anatomie Pathologique, Hôpital Haut-Levêque, CHU Bordeaux.
Chartois-Leauté
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AG
Dubus
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Duchayne
E
E
Brousset
P
P
Ceccaldi
J
J
Capbern
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Fialon
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Soubeyran
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Delsol
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de Mascarel
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Merlio
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JP
D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D019254:Genes, bcl-2; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008297:Male; D013160:Splenic Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D013163:Splenomegaly / Q000276:immunology / Q000473:pathology
D015703:Antigens, CD; D000951:Antigens, Neoplasm