Peptide antigen treatment of naive and virus-immune mice: antigen-specific tolerance versus immunopathology.
Peptide-specific down-regulation of T cell responses may represent a powerful tool to intervene in autoimmune diseases or graft rejections. It is therefore important to know whether peptide treatment tolerizes both naive and antigen-experienced memory T lymphocytes. Here we show that a major histocompatibility complex class I binding peptide, derived from the glycoprotein (GP33 peptide) of lymphocytic choriomeningitis virus (LCMV), specifically tolerized naive cytotoxic T lymphocytes (CTL) when administered three times intraperitoneally in incomplete Freund's adjuvants. However, in the presence of GP33-specific memory CTL in LCMV-primed mice, the same treatment had a general immunosuppressive effect on unrelated third-party antigen-specific T cell responses and caused severe immunopathological damage to the spleen.
Aichele
P
P
Institute of Experimental Immunology, Department of Pathology, University of Zurich, Switzerland.
Brduscha-Riem
K
K
Oehen
S
S
Odermatt
B
B
Zinkernagel
R M
RM
Hengartner
H
H
Pircher
H
H
D019264:Adoptive Transfer; D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D000939:Epitopes / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology* / Q000494:pharmacology; D007108:Immune Tolerance / Q000187:drug effects; D007156:Immunologic Memory / Q000187:drug effects; D007274:Injections, Intraperitoneal; D008213:Lymphocyte Activation / Q000187:drug effects; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000276:immunology* / Q000494:pharmacology; D013154:Spleen / Q000276:immunology / Q000473:pathology* / Q000637:transplantation; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014764:Viral Proteins
D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D010446:Peptide Fragments; D014764:Viral Proteins; C094244:glycoprotein peptide 33-41, Lymphocytic choriomeningitis virus