Identification and characterization of the SMT3 cDNA and gene from nematode Caenorhabditis elegans.
SMT3 gene from nematode, Caenorhabditis elegans, was identified and sequenced. The nematode SMT3 gene codes for a homolog to the yeast SMT3, suppressor of MIF2 mutation in a centromere protein gene. Further, nematode SMT3 cDNA was amplified by polymerase chain reaction from a cDNA library and its nucleotide sequence determined. A comparison of the SMT3 genomic and cDNA sequences established that the protein-encoding sequence is interrupted by two introns of 56 and 50 bp at codon Nos. 22-23 and 56, respectively. The sequence of 91 amino acids deduced from the nematode SMT3 nucleotide sequence exhibited 42 and 47% identity to the yeast and human SMT3 protein sequences, respectively. The evolutionary relationships of SMT3 proteins from human, nematode, Arabidopsis, rice, and yeast were analyzed.
Choudhury
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BK
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA.
Li
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SS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D018076:DNA, Complementary; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D025841:Small Ubiquitin-Related Modifier Proteins
D018076:DNA, Complementary; D012097:Repressor Proteins; C099362:SMT3 protein, S cerevisiae; C117909:SUMO2 protein, human; D029701:Saccharomyces cerevisiae Proteins; D025841:Small Ubiquitin-Related Modifier Proteins