title: Liquid chromatographic determination of the glycoalkaloids alpha-solanine and alpha-chaconine in potato tubers: NMKL Interlaboratory Study. Nordic Committee on Food Analysis.
abstract: Twelve laboratories participated in a collaborative study to evaluate precision parameters of a liquid chromatographic method for analysis of the glycoalkaloids alpha-solanine and alpha-chaconine in potato tubers. Samples consisted of frozen potato tuber homogenates distributed as 3 blind duplicates and 3 split-level pairs. The analytical method included aqueous extraction, workup on disposable solid-phase extraction cartridges, and reversed-phase chromatography with photometric detection at 202 nm. Results for alpha-solanine and alpha-chaconine were received from 10 and 9 laboratories, respectively. Relative standard deviations for reproducibility for alpha-solanine and alpha-chaconine were similar, ranging from 8 to 13% in the applied concentration range of 12 to 260 mg/kg fresh weight.
authors.affiliation: National Food Administration, Chemistry Division 3, Uppsala, Sweden.
authors.forename: K E
authors.initials: KE
authors.lastname: Hellenäs
authors.forename: C
authors.initials: C
authors.lastname: Branzell
mesh_terms: D002853:Chromatography, Liquid; D005504:Food Analysis; D007753:Laboratories; D015203:Reproducibility of Results; D012992:Solanine / Q000031:analogs & derivatives* / Q000032:analysis*; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D012992:Solanine; C011860:alpha-chaconine