title: Location of the three disulfide bonds in an antimicrobial peptide from Amaranthus caudatus using mass spectrometry.
abstract: The disulfide bridge pairing of Ac-AMP2, a 30-residue peptide isolated from the seeds of Amaranthus caudatus, is determined using a fast method involving enzymatic fragmentation followed by identification of the fragments with FAB mass spectrometry. The results confirm the location of the three disulfide bridges as previously established by NMR spectroscopy and molecular modelling.
authors.affiliation: Organic Chemistry Department, Vrije Universitet Brussel, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: el Bouyoussfi
authors.forename: G
authors.initials: G
authors.lastname: Laus
authors.forename: P
authors.initials: P
authors.lastname: Verheyden
authors.forename: L
authors.initials: L
authors.lastname: Wyns
authors.forename: D
authors.initials: D
authors.lastname: Tourwe
authors.forename: G
authors.initials: G
authors.lastname: Van Binst
mesh_terms: D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000737:chemistry*; D004220:Disulfides; D010450:Endopeptidases; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods
chemical_list: D000890:Anti-Infective Agents; D004220:Disulfides; D010446:Peptide Fragments; D010455:Peptides; D010450:Endopeptidases