Polymer-shielded dye-affinity chromatography.
Polymer-shielded dye-affinity chromatography is a form of chromatography in which the dye matrix forms complexes with a nonionic, water-soluble polymer such as poly(vinylpyrrolidone) or poly(vinyl alcohol), prior to the column chromatography of a crude protein extract, the idea being that polymer shielding of the dye will prevent nonspecific interactions between the target protein and the dye. The concept of polymer shielding and a strategy for the rational selection of polymers suitable as shielding agents are presented.
Mattiasson
B
B
Department of Biotechnology, Lund University, Sweden.
Galaev IYu
Garg
N
N
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D014227:Triazines / Q000737:chemistry
D004396:Coloring Agents; D011108:Polymers; D011506:Proteins; D014227:Triazines; C012842:Cibacron Blue F 3GA