Nuclear factor-kappaB activity and arterial response to balloon injury.
We studied the effect of arterial balloon injury on nuclear factor-kappaB (NF-kappaB) mobilization and ICAM-1 expression in untreated rats and rats treated with aspirin. Baseline NF-kappaB nuclear binding in smooth muscle cells (SMC) increased two-fold within 6 h after balloon injury. The binding returned to baseline 3 days after injury. Consistently nuclear staining of p65 active subunit increased in the medial SMC following balloon injury. There was no baseline ICAM-1 expression. Within 3 days after balloon injury there was marked medial ICAM-1 expression, that localized to neointima 7 days after injury and to regrowing endothelial cells 14 days after injury. Treatment with aspirin inhibited NF-kappaB nuclear translocation and binding and was associated with reduction of ICAM-1 expression, SMC proliferation and neointimal thickening following balloon injury. These data suggest that transient mobilization of NF-kappaB in vascular SMC after balloon injury mediates ICAM-1 expression and is involved in arterial response to balloon injury.
Cercek
B
B
Atherosclerosis Research Center, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Yamashita
M
M
Dimayuga
P
P
Zhu
J
J
Fishbein
M C
MC
Kaul
S
S
Shah
P K
PK
Nilsson
J
J
Regnstrom
J
J
D000818:Animals; D001158:Arteries / Q000293:injuries*; D001241:Aspirin / Q000494:pharmacology; D002404:Catheterization / Q000009:adverse effects*; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000648:ultrastructure; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1; D001241:Aspirin