title: Changes in gut neurotensin and modified colonic motility following whole-body irradiation in rat.
abstract: Exposure to ionizing radiation induces gastrointestinal dysfunction often associated with disorders of intestinal motility. Neurotensin is one of the mediators involved in the control of intestinal muscle activity. The aim of this study was to relate neurotensin tissue content and specific receptor binding with contractile effect of neurotensin in rat colon after irradiation. Rats were exposed to whole-body gamma-irradiation (60Co; 6 Gy). Intestinal (caecum, colon) neurotensin-like immunoreactivity, colonic muscle neurotensin receptor binding and neurotensin-induced contractions in isolated colon were investigated 3 and 7 days after irradiation. Irradiation produced a marked increase in the intestinal muscle content of neurotensin-like immunoreactivity (2.5-fold in caecum, 5-fold in colon) 3 days post-irradiation. At 7 days, the intestinal neurotensin content was close to that of the control values. Three days after irradiation, neurotensin receptors in colonic muscle were characterized by the appearance of a transient second class of sites of low affinity-high capacity. A three-fold increase in the total number of sites was observed. In addition, effects of neurotensin on isolated colon preparations showed an increase (37%) of potency but a decrease (7-fold) of efficacy. Seven days after irradiation, the efficacy was close to the control. Modifications of intestinal neurotensin content and specific receptor characteristics induced by irradiation can influence the colonic contractile activity.
authors.affiliation: Institut de Protection et de Sûreté Nucléaire, Département de Protection de la Santé de l'Homme et de Dosimétrie, Section Autonome de Radiobiologie Appliquée à la Médecine, IPSN, Fontenay aux Roses, France.
authors.forename: C
authors.initials: C
authors.lastname: Linard
authors.forename: N M
authors.initials: NM
authors.lastname: Griffiths
authors.forename: V
authors.initials: V
authors.lastname: Esposito
authors.forename: J
authors.initials: J
authors.lastname: Aigueperse
authors.forename: P
authors.initials: P
authors.lastname: Gourmelon
mesh_terms: D000818:Animals; D003106:Colon / Q000502:physiology / Q000528:radiation effects; D005769:Gastrointestinal Motility / Q000187:drug effects*; D007422:Intestines / Q000737:chemistry / Q000528:radiation effects*; D008297:Male; D009496:Neurotensin / Q000032:analysis* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018028:Receptors, Neurotensin / Q000032:analysis; D014916:Whole-Body Irradiation
chemical_list: D018028:Receptors, Neurotensin; D009496:Neurotensin