Long-term alterations in growth factor mRNA expression following seizures.
Although alterations in growth factor mRNA occur during neuronal insults, little is known about the long-term effects of neuronal insults on growth factor expression. We have examined the effects of prolonged post-ictal times on the expression of Brain-derivered nerve factor (BDNF) and Neurotrophin 3 (NT3) following Kainic acid (KA)-induced seizures. In situ hybridization was performed on male Sprague-Dawley rats sacrificed 1-2 weeks following intracranial ventricular KA injections. BDNF mRNA increased bilaterally 1 and 2 weeks after injections, whereas NT3 mRNA decreased contralaterally 1 week and bilaterally 2 weeks post-injection. These observations provide evidence that alterations in growth factor mRNA expression occur even after prolonged post-ictal recovery suggesting a possible role for growth factors in recovery and continued maintenance of surviving neurons within limbic seizure foci.
Garcia
M L
ML
Department of Molecular Neuroscience, Mayo Clinic Rochester, MN 55905, USA.
Garcia
V B
VB
Isackson
P J
PJ
Windebank
A J
AJ
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis; D013006:Growth Hormone / Q000235:genetics*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009414:Nerve Growth Factors / Q000096:biosynthesis; D020933:Neurotrophin 3; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000378:metabolism*; D013997:Time Factors
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D012333:RNA, Messenger; D013006:Growth Hormone