Long-term regulation of opioid receptors in neuroblastoma and lymphoma cell lines.
Long-term regulation of opioid binding was studied in the human neuroblastoma NMB and in the murine lymphoma R1.1 and R1.EGO cell lines. Binding was down-regulated following prolonged exposure to opioid agonists and up-regulated following exposure to antagonist. Down-regulation was inhibited by the metabolic blocker sodium-azide and by the protein kinase H-7. Up-regulation was blocked by the protein and mRNA synthesis blockers cycloheximide, alpha-amanitin and actinomycin D. A significant difference was found between the response of neuronal and immune cells to ethanol exposure: while opioid binding in neuroblastoma culture underwent a pronounced (75%) up-regulation, no effect of ethanol on opioid receptors in lymphoma cultures was detected. The described cell lines present an excellent experimental model to study long-term regulation of opioid receptors in the nervous and immune systems and to elucidate the biological effects of chronic use of opiates and alcohol.
Shapira
M
M
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Baumhaker
Y
Y
Sarne
Y
Y
D004174:Diprenorphine / Q000378:metabolism; D015536:Down-Regulation; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D008223:Lymphoma / Q000737:chemistry*; D009447:Neuroblastoma / Q000737:chemistry*; D011957:Receptors, Opioid / Q000032:analysis* / Q000187:drug effects; D014407:Tumor Cells, Cultured
D011957:Receptors, Opioid; D004174:Diprenorphine; D000431:Ethanol