title: Sematilide blocks the inward rectifier potassium channel in isolated guinea pig ventricular myocytes.
abstract: 1. In whole-cell patch recording, the relative potency of the blocking action of sematilide on IK1 was found to be constant at each potential level of IK1 activation. Under more acidic condition, the degree of block was decreased. These results strongly suggested that the neutral form of sematilide may penetrate the cardiac cell membrane via hydrophobic pathway. 2. In cell-attached patches, sematilide prolonged the interburst interval and reduced the opening probabilities of the IK1 channel without affecting either the mean open time or the mean closed time within a burst.
authors.affiliation: First Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takai
authors.forename: R
authors.initials: R
authors.lastname: Sato
authors.forename: R
authors.initials: R
authors.lastname: Katori
mesh_terms: D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers; D024661:Potassium Channels, Inwardly Rectifying; D011342:Procainamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D016276:Ventricular Function
chemical_list: D000889:Anti-Arrhythmia Agents; D026902:Potassium Channel Blockers; D024661:Potassium Channels, Inwardly Rectifying; C062818:sematilide; D011342:Procainamide