Genetic and physical delineation of the region of the mouse deafness mutation shaker-2.
A total of 951 backcross progeny have been obtained from a backcross segregating for the mouse deafness mutation, shaker-2(sh-2). Linkage analysis provides a detailed genetic map in the vicinity of sh-2 which comprises 40 backcross mice identified as recombinant within a 4 cM region. This allows construction of a contig consisting of 21 BAC clones across an approximately 700-kb region of sh-2. This covers the entire nonrecombinant region of sh-2 and is therefore useful to facilitate the identification of genes in the sh-2 region.
Wakabayashi
Y
Y
First Department of Biochemistry, Niigata University School of Medicine, Japan.
Kikkawa
Y
Y
Matsumoto
Y
Y
Shinbo
T
T
Kosugi
S
S
Chou
D
D
Furuya
M
M
Jishage
K
K
Noda
T
T
Yonekawa
H
H
Kominami
R
R
D000818:Animals; D002874:Chromosome Mapping; D016386:Chromosome Walking; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D003638:Deafness / Q000235:genetics*; D005808:Genes, Recessive; D008040:Genetic Linkage / Q000235:genetics; D006239:Haplotypes / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation; D016133:Polymerase Chain Reaction