Involvement of Ras in the expression of glycolipid sulfotransferase in human renal cancer cells.
Glycolipid sulfotransferase activity in a human renal cell carcinoma cell line, SMKT-R3, is enhanced by epidermal growth factor (EGF); tyrosine kinase inhibitors suppress this enhancement. To investigate the involvement of Ras in the signal transduction pathway from the EGF receptor to the expression of glycolipid sulfotransferase, we introduced v-H-ras into SMKT-R3 cells. In a quiescent state, the percent GTP bound to Ras in v-H-ras-expressing cells increased about 2.5-fold compared with control cells, suggesting that v-Ras introduced into the renal cancer cells is in an active form without EGF stimulation. Glycolipid sulfotransferase activity in v-H-ras-expressing cells was higher than in control cells. The sulfotransferase activity was affected neither by EGF nor by genistein, a tyrosine kinase inhibitor, in v-H-ras-expressing cells, whereas it was enhanced by EGF and reduced by genistein in control cells. Our observations suggest that Ras mediates the regulation pathway of glycolipid sulfotransferase activity in SMKT-R3 cells.
Yabunaka
N
N
Laboratory of Biochemistry, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
Honke
K
K
Ishii
A
A
Ogiso
Y
Y
Kuzumaki
N
N
Agishi
Y
Y
Makita
A
A
D002292:Carcinoma, Renal Cell / Q000201:enzymology / Q000235:genetics*; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D011905:Genes, ras; D019833:Genistein; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D007680:Kidney Neoplasms / Q000201:enzymology / Q000235:genetics*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D015689:Oncogene Protein p21(ras) / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D015398:Signal Transduction / Q000502:physiology*; D015238:Sulfotransferases / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
D004791:Enzyme Inhibitors; D007529:Isoflavones; D009363:Neoplasm Proteins; D004815:Epidermal Growth Factor; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D015238:Sulfotransferases; C020556:galactosylceramide sulfotransferase; D015689:Oncogene Protein p21(ras)