Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase.
A chloramphenicol acetyltransferase from Pseudomonas aeruginosa genomic DNA has been overexpressed, refolded, purified, and crystallized. Crystals suitable for a three-dimensional x-ray structure determination were obtained from solutions of polyethyleneglycol methyl ether 2000 containing NiCl2 at pH 8.5. These crystals belong to the cubic space group P4(1/3)32 (a = 154.8 A) and diffract x-rays to approximately 3.2 A resolution.
Tian
Y
Y
Department of Biochemistry, Albert Einstein College of Medicine, The Bronx, New York 10461, USA.
Beaman
T W
TW
Roderick
S L
SL
D015500:Chloramphenicol O-Acetyltransferase / Q000737:chemistry / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase