Human placental ecto-enzymes: studies on the plasma membrane anchorage and effect of inhibitors of ATP-metabolizing enzymes.
The human placental microvillar membrane contains several ectoenzymes, including 5'-nucleotidase, alkaline phosphatase and ATP-diphosphohydrolase (ATP-DPH), which might be involved in the extracellular metabolism of nucleotides. The type of anchorage to the plasma membrane of the two first enzymes has been shown to be via a glycosyl-phosphatidylinositol. In the present study, using an enzymatic approach, we show that the ATP-DPH should be attached to the plasma membrane through a different type of anchorage. We were also interested in the search of compounds which could interact differentially with this enzyme to be used as a tool for studying the other two hydrolytic enzymes in the presence of ATP-DPH. Here we report several inhibitors of ecto-ATPases which seem to be a useful tool for studying these three enzymes.
Chayet
L
L
Dept. de Bioquímica y Biología Molecular, Fac. Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
Collados
L
L
Kettlun
A M
AM
Campos
E
E
Traverso-Cori
A
A
García
L
L
Valenzuela
M A
MA
D015720:5'-Nucleotidase / Q000032:analysis* / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000032:analysis* / Q000378:metabolism; D000639:Amitriptyline / Q000494:pharmacology; D001081:Apyrase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D002110:Caffeine / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D010450:Endopeptidases / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D005476:Fluphenazine / Q000494:pharmacology; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D006801:Humans; D008012:Lidocaine / Q000494:pharmacology; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D009711:Nucleotides / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010739:Phospholipase D / Q000494:pharmacology; D010920:Placenta / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D010738:Type C Phospholipases / Q000494:pharmacology
D004791:Enzyme Inhibitors; D017261:Glycosylphosphatidylinositols; D009711:Nucleotides; D000639:Amitriptyline; D002110:Caffeine; D008012:Lidocaine; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D010738:Type C Phospholipases; D010739:Phospholipase D; D010450:Endopeptidases; D001081:Apyrase; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D005476:Fluphenazine