title: [Von Willebrand factor concentrations in blood plasma of Bernese mountain dogs].
abstract: Many Bernese Mountain dogs have been found to exhibit an increased hemorrhagic tendency of unknown etiology. Since other bleeding disorders have been excluded by routine tests and Bernese Mountain dogs have been listed to have von Willebrand's disease (vWD), we analyzed the plasma concentration of the von Willebrand Factor (vWF) in 160 Bernese Mountain dogs that were used for breeding in Switzerland in 1992. We also evaluated the suitability of the commercial Asserachrom vWF test kit to quantitate vWF in canine plasma by comparing the plasma vWF determination with validated vWF ELISA test. The vWF plasma concentration in Bernese Mountain dogs ranged from 13% to 162%, with the Asserachrom test kit (normal range 67% to 124%). Similar values were obtained with the research vWF ELISA kit (10% to 166%), and there was a close correlation between the two test methods. In 8 of the 9 Bernese Mountain dogs with initially low vWF concentration (< 60%), the determination was repeated on another sample. Since the values were well within the normal range, a problem with the collection of blood for the first determination is suspected. We conclude that vWD does not appear to be a clinical issue in Bernese Mountain dogs in Switzerland and is, therefore, not likely to be the cause of the observed bleeding tendency. The commercially available Asserachrom vWF test kit seems suitable for the determination of canine vWF plasma concentrations. It is recommended that low vWF values will be confirmed by the determination in second samples.
authors.affiliation: Departement für Fortpflanzungskunde, Veterinär-Medizinische Fakultät, Universität Zürich.
authors.forename: S
authors.initials: S
authors.lastname: Arnold
authors.forename: A
authors.initials: A
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Binder
authors.forename: K
authors.initials: K
authors.lastname: Meyers
authors.forename: U
authors.initials: U
authors.lastname: Giger
mesh_terms: D000818:Animals; D001947:Breeding; D004283:Dog Diseases / Q000453:epidemiology* / Q000235:genetics; D004285:Dogs; D013557:Switzerland / Q000453:epidemiology; D014842:von Willebrand Diseases / Q000453:epidemiology / Q000235:genetics / Q000662:veterinary*; D014841:von Willebrand Factor / Q000032:analysis*
chemical_list: D014841:von Willebrand Factor