title: Study of internal transcribed spacer and mitochondrial large-subunit genes of Pneumocystis carinii hominis isolated by repeated bronchoalveolar lavage from human immunodeficiency virus-infected patients during one or several episodes of pneumonia.
abstract: The objective of this study was to type, analyze, and compare Pneumocystis carinii hominis strains obtained from different samples during a given or recurrent episodes of P. carinii pneumonia (PCP) for epidemiologic purposes. We studied 36 bronchoalveolar lavage (BAL) or induced sputum (IS) samples from 16 human immunodeficiency virus-infected patients with one or several episodes of PCP. PCR amplification and direct sequencing were performed on the two internal transcribed spacers (ITS1 and ITS2) of P. carinii hominis rRNA genes by using DNA extracted from BAL or IS samples, and the sequences were compared to the mitochondrial large-subunit (mt LSU) gene sequence determined in a previous study in our laboratory. The studies of the mt LSU and ITS sequences showed that some patients (n = 10) were infected with the same strains of P. carinii hominis during a given episode of PCP. In one patient infected with strains with identical sequences in several episodes, the recurrence could have been due to reactivation of organisms not eliminated by treatment during the first episode or to de novo infection by an identical strain. In five patients infected with strains with different sequences in each episode, recurrence was due to de novo infection. Sequence analysis of these two P. carinii hominis gene regions showed that de novo infection can occur in AIDS patients with recurrent PCP.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, CHU Saint-Antoine, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Latouche
authors.forename: J L
authors.initials: JL
authors.lastname: Poirot
authors.forename: C
authors.initials: C
authors.lastname: Bernard
authors.forename: P
authors.initials: P
authors.lastname: Roux
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D018893:Bronchoalveolar Lavage; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D005798:Genes, Bacterial; D015497:HIV-1; D006801:Humans; D011010:Pneumocystis / Q000235:genetics / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology*
chemical_list: D004272:DNA, Mitochondrial