Effect of boric acid solution on cartilage metabolism.
Pelvic cartilage of chick embryo was used to demonstrate that presence of boron in culture medium decreases synthesis of proteoglycans, collagen and total proteins but on the other hand increases the release of these macromolecules. However, when glucose concentration in culture medium is brought to 22mM, the synthesis decrease is no longer observed, whereas release increase persists. Proteins released into the culture medium included heat shock proteins (70 hsp) and tumor necrosis factor alpha (TNF alpha). The amount of phosphorylated proteins was enhanced in presence of boron while endoprotease activity in cartilage and in culture medium was significantly augmented. The in vitro effects of boric acid may explain its in vivo effect on wound healing.
Benderdour
M
M
Laboratory of Medical Biochemistry-School of Medicine, University Nancy I, Henri Poincare, Vandoeuvre, France.
Hess
K
K
Gadet
M D
MD
Dousset
B
B
Nabet
P
P
Belleville
F
F
D000818:Animals; D001888:Boric Acids / Q000494:pharmacology*; D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques; D003545:Cysteine / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D010766:Phosphorylation / Q000187:drug effects; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D011509:Proteoglycans / Q000096:biosynthesis / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D001888:Boric Acids; D003470:Culture Media; D018840:HSP70 Heat-Shock Proteins; D011506:Proteins; D011509:Proteoglycans; D014409:Tumor Necrosis Factor-alpha; D003094:Collagen; D011392:Proline; D003545:Cysteine; D005944:Glucosamine; C032688:boric acid