title: Inhibition of glucose transport in human red blood cells by adenosine antagonists.
abstract: Previous studies have suggested that adenosine antagonists can interfere with normal glucose uptake in perfused rat heart. In the present studies, fluorine-19 nuclear magnetic resonance spectroscopy was used to study the effect of the adenosine antagonist, BW-A1433U, on the equilibrium exchange of fluorinated glucose analogs in human erythrocytes. Studies of the equilibrium exchange of both 2-fluoro-2-deoxy-D-glucose and 3-fluoro-3-deoxy-D-glucose with either one-dimensional magnetization transfer or two-dimensional exchange spectroscopy were performed, and significant inhibition was observed in all cases. From concentration-dependent studies, an inhibition constant for the equilibrium exchange measured at 37 degrees C of 24 microM was determined.
authors.affiliation: Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Gabel
authors.forename: T M
authors.initials: TM
authors.lastname: O'Connell
authors.forename: E
authors.initials: E
authors.lastname: Murphy
authors.forename: R E
authors.initials: RE
authors.lastname: London
mesh_terms: D000241:Adenosine / Q000037:antagonists & inhibitors*; D001692:Biological Transport / Q000187:drug effects; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D005462:Fluorine Radioisotopes; D019788:Fluorodeoxyglucose F18; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D008954:Models, Biological; D014970:Xanthines / Q000494:pharmacology*
chemical_list: D005462:Fluorine Radioisotopes; D014970:Xanthines; D019788:Fluorodeoxyglucose F18; C053414:BW A1433U; D003847:Deoxyglucose; D000241:Adenosine