Lactose-induced expression of Bacillus sp. TS-23 amylase gene in Escherichia coli regulated by a T7 promoter.
Bacillus sp. TS-23 amylase gene was amplified by polymerase chain reaction and cloned into the pET23(+) transcription vector. Lactose induction of Escherichia coli BL21(DE3) cells harbouring this recombinant plasmid resulted in the extracellular production of gene products. By the addition of 20 mmol l-1 lactose, the amylase activity of fermentation broth had a specific activity of 21 U mg-1 of protein.
Lin
L L
LL
Department of Food Science and Nutrition, Hung Kuang Institute of Medical and Nursing Technology, Taichung, Taiwan.
Hsu
W H
WH
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial / Q000187:drug effects; D007785:Lactose / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D000516:alpha-Amylases / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; D000516:alpha-Amylases; D007785:Lactose