title: Yellow fever virus envelope protein has two discrete type-specific neutralizing epitopes.
abstract: Two monoclonal antibody neutralization resistant (MAbR) variants of the yellow fever (YF) 17D-204 vaccine virus strain were selected using YF type-specific MAb B39. These B39R variants were compared with the variant virus selected by Lobigs et al. (Virology 161, 474-478, 1987) using a second YF-type specific MAb (2E10) which mapped to amino acid position 71/72 in the envelope (E) protein. Neutralization assays with a panel of MAbs suggested that these two YF-type-specific epitopes are located in two discrete regions of the folded E protein. Each of the B39R variants had a single nucleotide mutation which encoded an amino acid substitution at either position E-155 or E-158. Thus, YF type-specific epitopes map to both domain I (B39) and II (2E10) of the YF virus E protein. The B39 defined epitope represents the first flavivirus neutralizing epitope localized to this region of domain I of the E protein.
authors.affiliation: Center for Tropical Diseases, University of Texas Medical Branch, Galveston 77555, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Ryman
authors.forename: T N
authors.initials: TN
authors.lastname: Ledger
authors.forename: R C
authors.initials: RC
authors.lastname: Weir
authors.forename: J J
authors.initials: JJ
authors.lastname: Schlesinger
authors.forename: A D
authors.initials: AD
authors.lastname: Barrett
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000276:immunology*; D014774:Virulence; D015005:Yellow fever virus / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000939:Epitopes; D014759:Viral Envelope Proteins