Characterization of Marek's disease herpesvirus-specific cytotoxic T lymphocytes in chickens inoculated with a non-oncogenic vaccine strain of MDV.
Previously we have reported that reticuloendotheliosis virus (REV)-transformed cell lines expressing Marek's disease virus (MDV) genes pp38, meq or gB were lysed by syngeneic MDV-specific splenocytes from major histocompatibility complex (MHC):B9B19 and MHC:B1B21 chickens. In contrast, REV-transformed cell lines expressing the MDV gene ICP4 were only lysed by syngeneic MDV-specific splenocytes from MHC:B21B21 chickens. In this study we report that this syngeneic cell-mediated immune response is induced by cytotoxic T lymphocytes (CTL). Splenocytes from MDV vaccine strain, SB-1 inoculated MHC:B19B19 and MHC:B21B21 chickens, depleted for CD4+, CD8+, TCR gamma delta +, TCR alpha beta 1+ and/or TCR alpha beta 2+ cells, were used as effector cells in chromium-release assays. Effector cells depleted of CD8+ or TCR alpha beta 1+, but not TCR gamma delta + or TCR alpha beta 2+, markedly reduced the MDV-specific release. Depletion of CD4+ effector cells did not influence the specific release significantly. This is the first report on identification of virus-specific CD8+ CTL in chickens inoculated with a non-oncogenic vaccine strain of MDV.
Omar
A R
AR
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Schat
K A
KA
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018929:Cell Culture Techniques; D002460:Cell Line; D002645:Chickens / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology; D008381:Herpesvirus 2, Gallid / Q000276:immunology; D016130:Immunophenotyping; D008380:Marek Disease / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology
D000939:Epitopes