Three-dimensional culture of a mixed mullerian tumor of the ovary: expression of in vivo characteristics.
The Rotating-Wall Vessel (RWV) is a novel in vitro cell culture system used to successfully culture a cell line derived from a heterologous mixed mullerian tumor cell of the ovary. Although the original tumor was comprised of both epithelial and mesodermal components, long-term culture in conventional flasks established a cell line from this tumor with homogeneous epitheliallike growth characteristics (1). Cells from Passage 36 were seeded into a Rotating-Wall Vessel containing Cytodex-3 microcarrier beads. Scanning electron micrographs of tumor cells cultured for 32 d in the RWV showed the presence of heterogeneous cell populations organized into three-dimensional tissuelike architecture. Immunocytochemical analysis confirmed the cellular heterogeneity, as demonstrated by expression of both epithelial and mesenchymal antigens. Reverse transcription polymerase chain reaction amplification demonstrated the presence of mRNA for cellular oncogenes HER-2/neu, H-ras, K-ras, and tumor suppressor p53. Thus, there are two advantages to propagation of tissue in the RWV culture system:(a) tissue diversification representing populations present in the original tumor, and (b) the three-dimensional freedom to organize tissues morphologically akin to those observed in vivo. These data indicate that the RWV culture system is suitable for generating large quantities of ovarian tumor cells in vitro that are amenable to immunocytochemical, oncogenic, morphologic characteristics demonstrated in vivo.
Goodwin
T J
TJ
NASA/Johnson Space Center, Biotechnology, Houston, Texas 77058, USA.
Prewett
T L
TL
Spaulding
G F
GF
Becker
J L
JL
D019149:Bioreactors; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002455:Cell Division; D005260:Female; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D008855:Microscopy, Electron, Scanning; D018200:Mixed Tumor, Mullerian / Q000648:ultrastructure; D010051:Ovarian Neoplasms / Q000648:ultrastructure; D011519:Proto-Oncogenes; D014407:Tumor Cells, Cultured