title: Evolution of cell-surface acid phosphatase of Burkholderia pseudomallei.
abstract: Acid phosphatase active fractions were obtained from cell-free extract, outermembrane fraction and culture filtrate of Burkholderia pseudomallei by column chromatography with sepharose 6B and DEAE cellulose. The comparison of the elution patterns of protein, sugar and enzymatic activity among these three components suggested that the enzyme is a glycoprotein evolving from premature proteins through glycosylation and that the enzyme is translocated during glycosylation from the cytoplasm to the outer membrane and finally excreted into the environment. When tunicamycin, a glycosylation inhibitor, was added to the culture, the peaks of sugar and enzymatic activity were lowered concomitantly leaving the protein peak unchanged in the elution pattern of the culture filtrate. The affinity of the bacterial surface to antienzyme sera was demonstrated by immuno-fluorescence microscopy.
authors.affiliation: National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
authors.forename: E
authors.initials: E
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Kurata
authors.forename: P
authors.initials: P
authors.lastname: Naigowit
authors.forename: K
authors.initials: K
authors.lastname: Kanai
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D016957:Burkholderia pseudomallei / Q000201:enzymology*; D019084:Fluorescent Antibody Technique, Indirect; D006023:Glycoproteins; D006031:Glycosylation; D006801:Humans; D008554:Melioidosis / Q000382:microbiology*; D008856:Microscopy, Fluorescence; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D006023:Glycoproteins; D014415:Tunicamycin; D000135:Acid Phosphatase