Isolation of coding sequences from bovine cosmids by means of exon trapping.
Exon trapping was employed to identify coding sequences from a collection of 46 bovine cosmids, previously characterized for the presence of microsatellite markers and physically mapped to chromosomes by FISH. The sequence analysis of 104 clones revealed 18 putative exons, 10 of which showed near identity to known sequences. Among these were the human (cytosine-5)-methyltransferase (DNMT), ATP-citrate lyase (ACLY), the mouse Lbcl1 oncogene, the bovine mitochondrial aconitase (ACO2) and beta-arrestin 1 (ARR1). The chromosomal localization of the cloned exons was inferred from the localization of the parent cosmids. DNMT and ACLY were not previously known in cattle, but the physical localization of the cloned bovine exons is in agreement with the published comparative human and bovine maps. The trapping of exons for bovine ACO2 and ARR1 confirms the available mapping information based on synteny and provides a physical assignment for the genes.
Comincini
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Istituto per la Difesa e la Valorizzazione del Germoplasma Animale (I.D.V.G.A.) - C.N.R., via Celoria 10, 20133 Milano, Italy.
Drisaldi
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Ferretti
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D001275:ATP Citrate (pro-S)-Lyase / Q000235:genetics; D000154:Aconitate Hydratase / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D019390:Arrestins / Q000235:genetics; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids / Q000235:genetics*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics; D005091:Exons; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009857:Oncogenes / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D000071559:beta-Arrestin 1; D000071557:beta-Arrestins
C000605253:ARRB1 protein, human; C000605255:Arrb1 protein, mouse; D019390:Arrestins; D000071559:beta-Arrestin 1; D000071557:beta-Arrestins; D004248:DNA (Cytosine-5-)-Methyltransferases; D001275:ATP Citrate (pro-S)-Lyase; D000154:Aconitate Hydratase