title: Assessment of reperfusion injury by means of MR contrast agents in rat liver.
abstract: The purpose of this study was to investigate whether extracellular MR contrast agents or intracellular liver-specific MR contrast agents may enable the assessment of liver reperfusion injury. Ischemia-related reperfusion was induced in 32 rats using Pringle's maneuver. Pringle's maneuver consisted of cross-clamping of the complete hepatoduodenal ligament for 45 minutes followed by 90 minutes of reperfusion. Two extracellular (gadopentetate dimeglumine and gadobutrol) and two intracellular gadoxetic acid and SH U 555 A) MR contrast agents were evaluated as model agents. Control animals and animals with liver ischemia were used to calculate changes in liver signal enhancement after Pringle's maneuver. Significant changes in liver signal after reperfusion injury were observed only with reticuloendothelial system (RES)-specific SH U 555 A. Liver signal enhancement after Pringle's maneuver with RES-specific SH U 555 A was decreased by 25.4% as compared with the control group. RES-specific contrast agents such as SH U 555 A seem to be more sensitive to ischemia-related dysfunction of the liver than hepatobiliary contrast agents such as gadoxetic acid or extracellular gadolinium chelates at different concentrations because Kupffer cells are more sensitive to liver ischemia than hepatocytes.
authors.affiliation: Institute of Clinical Radiology, Westphalian Wilhelms University Münster, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Reimer
authors.forename: T
authors.initials: T
authors.lastname: Allkemper
authors.forename: C
authors.initials: C
authors.lastname: Bremer
authors.forename: E J
authors.initials: EJ
authors.lastname: Rummeny
authors.forename: H U
authors.initials: HU
authors.lastname: Spiegel
authors.forename: T
authors.initials: T
authors.lastname: Balzer
authors.forename: P E
authors.initials: PE
authors.lastname: Peters
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004338:Drug Combinations; D005260:Female; D019786:Gadolinium DTPA; D007089:Image Enhancement / Q000379:methods; D008099:Liver / Q000473:pathology*; D008297:Male; D008536:Meglumine / Q000008:administration & dosage*; D009942:Organometallic Compounds / Q000008:administration & dosage*; D004369:Pentetic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D015427:Reperfusion Injury / Q000175:diagnosis* / Q000473:pathology; D012680:Sensitivity and Specificity
chemical_list: D003287:Contrast Media; D004338:Drug Combinations; D009942:Organometallic Compounds; C090600:gadobutrol; D008536:Meglumine; D004369:Pentetic Acid; D019786:Gadolinium DTPA