Inhibitory activity of 3'-fluoro-2' deoxythymidine and related nucleoside analogues against adenoviruses in vitro.
Antiviral effects of nucleoside analogues against human adenoviruses (ADV) belonging to subgroup B (ADV3) and C (ADV2) were comparatively analysed using focus reduction assay on Fogh and Lund (FL) cells. 3'-Fluoro-2'-deoxythymidine (FTdR), 3'-fluoro-2'-deoxyuridine (FUdR), 2',3'-dideoxycytidine (ddC) and 3'-fluoro-2'-deoxyguanosine (FGdR) emerged as potent and selective inhibitors. They were nontoxic for the FL cells at the tested doses. FTdR was proved to be the most effective inhibitor against both serotypes ADV2 and ADV3 (0.05 microM/0.02 microM). The inhibitory effect of FTdR was also analyzed on the level of viral proteins and viral DNA synthesis using radioimmunoprecipitation and PCR, respectively. Neither the main structural protein of ADV, the hexon, nor viral DNA could be detected in ADV-infected FL cells that had been exposed to FTdR.
Mentel
R
R
Institut für Medizinische Mikrobiologie der Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Kinder
M
M
Wegner
U
U
von Janta-Lipinski
M
M
Matthes
E
E
D000260:Adenoviruses, Human / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D015224:Dideoxynucleosides / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D005467:Floxuridine / Q000494:pharmacology; D006801:Humans; D016133:Polymerase Chain Reaction; D014779:Virus Replication / Q000187:drug effects; D016047:Zalcitabine / Q000494:pharmacology
D000998:Antiviral Agents; D017931:DNA Primers; D004279:DNA, Viral; D015224:Dideoxynucleosides; D005467:Floxuridine; D016047:Zalcitabine; D003849:Deoxyguanosine; C002854:alovudine