Evidence for distinct behaviour of phosphatidylcholine and sphingomyelin at the low density lipoprotein surface.
This study demonstrates that the use of high field 1H NMR spectroscopy permits individual detection of phosphatidylcholine and sphingomyelin molecules at the surface of native low density lipoprotein (LDL) particles. Distinct behaviour was observed for the choline head group -N(CH3)3 resonances of these different phospholipids revealing preferential immobilisation for phosphatidylcholine. This suggests the existence of reversible and irreversible phosphatidylcholine-apolipoprotein B interactions and is consistent with microdomain formation at the surface monolayer of LDL. The novel resonance assignment and results show that 1H NMR can provide efficient and practical means for future studies on the structure and dynamics at the LDL surface.
Murphy
H C
HC
Medical Unit (Cellular and Molecular Mechanisms Research Group), St. Bartholomew's and The Royal London School of Medicine and Dentistry, United Kingdom.
Ala-Korpela
M
M
White
J J
JJ
Raoof
A
A
Bell
J D
JD
Barnard
M L
ML
Burns
S P
SP
Iles
R A
RA
D000328:Adult; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry*
D008077:Lipoproteins, LDL; D010713:Phosphatidylcholines; D013109:Sphingomyelins