Interferons alpha, beta and gamma induce different patterns of gene expression in cultured human epidermal keratinocytes.
Differences in the effects on confluent epidermal keratinocytes of treatment with interferons (IFNs) alpha, beta, and gamma were observed in their modulation of the mRNA levels of representative structural and functional cellular proteins. Comparisons of the responses in culture media with varying cellular maturation potential indicated the dependence of the modulation on the stage of differentiation. Differentiation was correlated with upregulation of all the genes by interferon gamma, but this effect was not seen with the other interferons. Even though IFNs alpha and beta share the same cell surface receptor, their effects on gene expression were clearly distinguishable and varied with the culture medium. These findings might have relevance in the treatment of skin lesions with varying degrees of differentiation.
Arany
I
I
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555-1019, USA.
Arany
M
M
Brysk
H
H
Tyring
S K
SK
Brysk
M M
MM
D016203:CDC2 Protein Kinase / Q000235:genetics; D002352:Carrier Proteins; D018797:Cell Cycle Proteins / Q000235:genetics; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000235:genetics; D056465:Retinoblastoma-Binding Protein 1; D012867:Skin / Q000166:cytology; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D016898:Interferon-alpha; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D016160:Retinoblastoma Protein; D056465:Retinoblastoma-Binding Protein 1; D050680:Transcription Factor DP1; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D016159:Tumor Suppressor Protein p53; D016899:Interferon-beta; D007371:Interferon-gamma; D007372:Interferons; D016203:CDC2 Protein Kinase