title: Mouse deoxyribonuclease I (DNase I): biochemical and immunological characterization, cDNA structure and tissue distribution.
abstract: Mouse urinary deoxyribonuclease I (DNase I) resembles rat and human DNase Is in terms of its proteochemical and enzymological properties. Furthermore, mouse DNase I was demonstrated to be immunologically closer to the rat than to the human enzyme. A 1176 bp full length cDNA encoding mouse DNase I was constructed from RNA obtained from the kidney and parotid glands. The amino acid sequence up to the 45th residue from the N-terminal of the mature enzyme was identical to that deduced from the cDNA sequence. This DNase I was distributed most densely in the parotid glands from the standpoint of both enzyme activity and gene transcript levels.
authors.affiliation: Department of Legal Medicine, Gunma University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: O
authors.initials: O
authors.lastname: Hosomi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakashima
authors.forename: K
authors.initials: K
authors.lastname: Kishi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007668:Kidney / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000201:enzymology; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014018:Tissue Distribution
chemical_list: D000906:Antibodies; D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA; D003850:Deoxyribonuclease I