[Mutagenicity studies of RCC-36, the active metabolite of (+/-)-4-diethylamino-1,1-dimethylbut-2-yn-1-yl 2-cyclohexyl-2-hydroxy-2-phenylacetate monohydrochloride monohydrate (NS-21), a novel drug for urinary frequency and incontinence].
The mutagenicity of (+/-)-4-ethylamino-1,1-dimethylbut-2-yn-1-yl 2-cyclohexyl-2-hydroxy-2-phenylacetate monohydrochloride (RCC-36), an active metabolite of (+/-)-4-diethylamino-1,1-dimethylbut-2-yn-1-yl 2-cyclohexyl-2-hydroxy-2-phenylacetate monohydrochloride monohydrate (NS-21), was investigated by the reverse mutation test in bacteria, the chromosome aberration test in vitro, and the micronucleus test in mice. The reverse mutation test was performed at a dose range of 6.25-400 micrograms/plate using Salmonella typhimurium TA100, TA1535, TA98, and TA1537, and Escherichia coli WP2uvrA. RCC-36 did not increase revertant colonies significantly in any of the test strains with or without metabolic activation system (S9 mix). The chromosome aberration test was carried out at a dose range of 2.5-20 micrograms/ml without S9 mix and 10-80 micrograms/ml with S9 mix using cultured Chinese hamster lung cells (CHL/IU). No significant increases of the frequencies of cells with chromosome aberrations were observed with or without S9 mix. The micronucleus test was conducted in the bone marrow cells of Slc:ddY male mice. Mice were given RCC-36 by a single intraperitoneal administration at doses of 0, 10, 20, 40, and 80 mg/kg. There were no significant increases in the frequencies of micronucleated polychromatic erythrocytes at any dose levels. These results show that RCC-36 has no mutagenic activity in vitro or in vivo.
Tamura
H
H
Research Laboratories, Nippon Shinyaku Co., Ltd, Kyoto, Japan.
Yamashita
Y
Y
Kitayama
E
E
Iwakura
K
K
Watanabe
M
M
Sumi
N
N
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D008297:Male; D051379:Mice; D015394:Molecular Structure; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010648:Phenylacetates / Q000737:chemistry / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development; D014549:Urinary Incontinence / Q000188:drug therapy; D014555:Urination Disorders / Q000188:drug therapy*
D009153:Mutagens; D010648:Phenylacetates; C107100:RCC 36; C105354:temiverine