HLA class I-restricted and tumor-specific cytotoxic T lymphocytes from metastatic lymph nodes of esophageal cancers.
This paper investigates the presence of HLA class I-restricted and tumor-specific cytotoxic T lymphocytes (CTL) in tumor sites of esophageal cancers. Five CTL lines were established from the metastatic lymph nodes or pleural effusion by incubation with interleukin-2 of tumor-infiltrating lymphocytes: cases 1 and 5, HLA-A26- and HLA-A33-restricted and squamous cell carcinoma (SCC)-specific CTL; case 2, HLA-Cw0102-restricted and esophageal SCC-specific CTL; case 3, HLA-A24- and HLA-A26-restricted CTL recognizing histologically different tumor cells; and case 4, HLA-A26-restricted and esophageal SCC-specific CTL. These results suggest the existence of HLA class I-restricted and tumor-specific CTL in metastatic esophageal SCC.
Toh
U
U
First Department of Surgery, Kurume University School of Medicine, Asahi-machi, Japan.
Yamana
H
H
Nakao
M
M
Imai
Y
Y
Seki
N
N
Takasu
H
H
Kaneshige
T
T
Fujita
H
H
Shirouzu
K
K
Itoh
K
K
D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology / Q000556:secondary*; D003601:Cytotoxicity Tests, Immunologic; D018076:DNA, Complementary / Q000235:genetics; D004938:Esophageal Neoplasms / Q000276:immunology / Q000473:pathology*; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology*; D059871:HLA-A24 Antigen; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008207:Lymphatic Metastasis / Q000276:immunology* / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000473:pathology; D009928:Organ Specificity; D016066:Pleural Effusion, Malignant / Q000276:immunology / Q000473:pathology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D015234:HLA-A Antigens; C039683:HLA-A*26 antigen; C082945:HLA-A*33 antigen; D059871:HLA-A24 Antigen; D007376:Interleukin-2