Murine Cyp1a-1 induction in mouse hepatoma Hepa-1C1C7 cells by myristicin.
Mouse hepatoma Hepa-1c1c7 (Hepa-1) cells were treated with myristicin to assess the role of myristicin in the process of Cyp1a-1 induction. Treatment of Hepa-1 cells with myristicin increased Cyp1a-1 transcription in a dose-dependent manner as shown by analysis of 7-ethoxyresorufin O-deethylase activity, Cyp1a-1 protein level, and Cyp1a-1 mRNA. Myristicin, however, did not competitively displace [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin from the Hepa-1 cytosolic aryl hydrocarbon (Ah) receptor in a competitive Ah receptor binding analysis using sucrose density gradient sedimentation and did not affect formation of DNA-protein complexes between the Ah receptor and its DRE target in a gel mobility shift assay using oligonucleotides corresponding to DRE 3 of the Cyp1a-1. These results suggest that the induction of Cyp1a-1 gene expression by myristicin in Hepa-1 cells might occur through an Ah receptor-independent pathway.
Jeong
H G
HG
Department of Biological Science, Chosun University, Kwangju, Korea.
Lee
S S
SS
Kim
H K
HK
Yang
K H
KH
D000081248:Allylbenzene Derivatives; D000818:Animals; D001593:Benzyl Compounds; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis* / Q000235:genetics; D004148:Dioxolanes / Q000378:metabolism / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism / Q000494:pharmacology; D011748:Pyrogallol / Q000031:analogs & derivatives; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000081248:Allylbenzene Derivatives; D001593:Benzyl Compounds; D004148:Dioxolanes; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D018336:Receptors, Aryl Hydrocarbon; D011748:Pyrogallol; C005246:myristicin; D019363:Cytochrome P-450 CYP1A1