PCR analysis of half-seeds of cereal crops and its application in marker-assisted selection and breeding.
A simple and rapid PCR method was adapted to DNA analysis of cereal seeds. Half-seeds of rice, wheat, and maize were treated with an extraction buffer and the resulting supernatants were used in PCR and RAPD reactions. PCR products amplified from the half-seed DNA extracts were identical with that from leaf tissue DNA extracts when the same specific primers were used in PCR reactions. The remaining half-seeds with embryos could still germinate normally. The half-seed PCR analysis was applied to the identification of resistant genotypes of rice bacterial blight, and it was proved effective in plant breeding and genetic studies.
Zhai
W
W
Institute of Genetics, Chinese Academy of Sciences, Beijing, China.
Lu
C
C
Zhu
L
L
Yang
W
W
Zhang
Q
Q
D001947:Breeding; D002523:Edible Grain / Q000196:embryology / Q000235:genetics* / Q000276:immunology; D005819:Genetic Markers; D005838:Genotype; D010935:Plant Diseases / Q000235:genetics; D016133:Polymerase Chain Reaction; D012639:Seeds; D012641:Selection, Genetic
D005819:Genetic Markers