Generation of small insert genomic FISH probes with high signal intensity suitable for deletion mapping.
We have developed a method to generate FISH probes from small (2-4 kb) nonrepetitive genomic restriction fragments. The probes showed a high hybridization efficiency of up to 90% in metaphase cells from normal peripheral blood. With the use of these probes, homozygous as well as hemizygous 9p deletions were reliably identified in nine leukemia-derived cell lines.
Dreyling
M H
MH
Abteilung für Hämatologie und Onkologie, Georg-August-Universität Götingen, Germany. mdreylin@mdv.gwdg.de
Olopade
O I
OI
Bohlander
S K
SK
D002460:Cell Line; D002872:Chromosome Deletion; D002899:Chromosomes, Human, Pair 9; D015342:DNA Probes; D018922:HL-60 Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016254:Mutagenesis, Insertional
D015342:DNA Probes