[Increase in access to SH-groups of membrane proteins in Ehrlich ascites carcinoma cells upon purinergic receptor activation].
The effect of ATP on SH-groups accessibility of the surface membrane proteins in Ehrlich ascites tumor cells has been studied using a fluorescent nonpenetrating reagent Thiolyte MQ. No accessible protein SH-groups in plasma membrane of unstimulated cells have been found. The accessibility of SH-groups in proteins with molecular masses 82 and 23 kDa was increased in 10 s after the ATP addition. The data are interpreted as a result of the conformational transition or vertical translocation of certain thiol-bearing membrane proteins at purinoreceptor activation. The effect may apparently be coupled to the changes in the membrane potential and calcium concentration in the cytoplasm occurring in the same time interval.
Rybina
V V
VV
Zinchenko
V P
VP
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D005456:Fluorescent Dyes; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D011983:Receptors, Purinergic / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D008565:Membrane Proteins; D011983:Receptors, Purinergic; D013438:Sulfhydryl Compounds; D002118:Calcium