Prp31p promotes the association of the U4/U6 x U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae.
The PRP31 gene encodes a factor essential for the splicing of pre-mRNA in Saccharomyces cerevisiae. Cell extracts derived from a prp31-1 strain fail to form mature spliceosomes upon heat inactivation, although commitment complexes and prespliceosome complexes are detected under these conditions. Coimmunoprecipitation experiments indicate that Prp31p is associated both with the U4/U6 x U5 tri-snRNP and, independently, with the prespliceosome prior to assembly of the tri-snRNP into the splicing complex. Nondenaturing gel electrophoresis and glycerol gradient analyses demonstrate that while Prp31p may play a role in maintaining the assembly or stability of tri-snRNPs, functional protein is not essential for the formation of U4/U6 or U4/U6 x U5 snRNPs. These results suggest that Prp31p is involved in recruiting the U4/U6 x U5 tri-snRNP to prespliceosome complexes or in stabilizing these interactions.
Weidenhammer
E M
EM
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Ruiz-Noriega
M
M
Woolford
J L
JL
D005656:Fungal Proteins / Q000378:metabolism*; D046911:Macromolecular Substances; D009694:Nucleic Acid Precursors / Q000378:metabolism; D012326:RNA Splicing; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000378:metabolism; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000378:metabolism / Q000648:ultrastructure*; D017415:Ribonucleoprotein, U5 Small Nuclear / Q000378:metabolism / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D017381:Spliceosomes / Q000378:metabolism / Q000648:ultrastructure*
D005656:Fungal Proteins; D046911:Macromolecular Substances; D009694:Nucleic Acid Precursors; C491451:PRP31 protein, S cerevisiae; D012331:RNA, Fungal; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D029701:Saccharomyces cerevisiae Proteins