Expression of the RT6 mono(ADP-ribosyl)transferases is regulated by two promoter regions.
The structure of the RT6 mono(ADP-ribosyl)transferase gene was studied. Analysis of cDNA clones revealed eight exons and suggested two independent transcriptional start sites. The existence of the downstream initiation site was confirmed by S1-nuclease protection and localized to position +29 of exon 2. The corresponding 5' flanking regions were found to contain typical promoter structures such as TATA- and CCAAT-boxes. Comparison with sequences deposited in the TRANSFAC database of transcription factor binding sites revealed few putative regulatory elements in the region associated with exon 1 (promoter 1). In contrast, several elements contained in the regulatory regions of other T cell-specific genes, such as ets, lyf-1 and ikaros were found in in promoter 2. Analysis of RT6-transcripts showed this region to be the most active promoter in spleen cells of adult rats. Finally, transient transfection assays with reporter gene constructs showed promoter 2 to mediate T-cell specific transcription.
Kuhlenbäumer
G
G
Department of Immunology, University Hospital Hamburg-Eppendorf, Germany.
Rothenburg
S
S
Matthes
M
M
Hollmann
C
C
Wingender
E
E
Thiele
H G
HG
Koch-Nolte
F
F
Haag
F
F
D036002:ADP Ribose Transferases / Q000235:genetics*; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics*; D005786:Gene Expression Regulation; D008562:Membrane Glycoproteins / Q000235:genetics*; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D051381:Rats; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D000945:Antigens, Differentiation, T-Lymphocyte; D008562:Membrane Glycoproteins; D036002:ADP Ribose Transferases; C090163:Art2b protein, rat