Preliminary investigations of an amperometric oligosaccharide dehydrogenase-based electrode for the detection of glucose and some other low molecular weight saccharides.
Biosensors for the determination of sugars were constructed using oligosaccharide dehydrogenase (ODH) and soluble phenazine methosulfate (PMS) or an osmium-based three-dimensional redox hydrogel. In the latter case the enzyme and poly(1-vinylimidazole) complexed with osmium (4,4'-dimethylbpy)2Cl were cross-linked with poly(ethylene glycol) diglycidyl ether. Both electrode configurations showed similar sensitivities for glucose in the range between 8 and 21 muAmM-1 cm-2. The responses for 10 mono and oligosaccharides were studied. There was no response for fructose. In the concentration range 0.1-20 mM the relative sensitivities were determined for arabinose (96%), xylose (3%), mannose (50%), galactose (11%), glucose (100%), maltose (24%), lactose (12%), cellobiose (34%) and maltotriose (10%).
Ruzgas
T
T
Enzyme Chemistry Laboratory, Institute of Biochemistry, Vilnius, Lithuania.
Csöregi
E
E
Katakis
I
I
Kenausis
G
G
Gorton
L
L
D015374:Biosensing Techniques; D002138:Calibration; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D008773:Methylphenazonium Methosulfate / Q000737:chemistry; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D009992:Osmium / Q000737:chemistry; D011108:Polymers; D012680:Sensitivity and Specificity
D009844:Oligosaccharides; D011108:Polymers; D008773:Methylphenazonium Methosulfate; D009992:Osmium; D002237:Carbohydrate Dehydrogenases; C107242:oligosaccharide dehydrogenase; D005947:Glucose