Liquid chromatographic determination of the glycoalkaloids alpha-solanine and alpha-chaconine in potato tubers: NMKL Interlaboratory Study. Nordic Committee on Food Analysis.
Twelve laboratories participated in a collaborative study to evaluate precision parameters of a liquid chromatographic method for analysis of the glycoalkaloids alpha-solanine and alpha-chaconine in potato tubers. Samples consisted of frozen potato tuber homogenates distributed as 3 blind duplicates and 3 split-level pairs. The analytical method included aqueous extraction, workup on disposable solid-phase extraction cartridges, and reversed-phase chromatography with photometric detection at 202 nm. Results for alpha-solanine and alpha-chaconine were received from 10 and 9 laboratories, respectively. Relative standard deviations for reproducibility for alpha-solanine and alpha-chaconine were similar, ranging from 8 to 13% in the applied concentration range of 12 to 260 mg/kg fresh weight.
Hellenäs
K E
KE
National Food Administration, Chemistry Division 3, Uppsala, Sweden.
Branzell
C
C
D002853:Chromatography, Liquid; D005504:Food Analysis; D007753:Laboratories; D015203:Reproducibility of Results; D012992:Solanine / Q000031:analogs & derivatives* / Q000032:analysis*; D011198:Solanum tuberosum / Q000737:chemistry*
D012992:Solanine; C011860:alpha-chaconine