MNDA dimerizes through a complex motif involving an N-terminal basic region.
Human myeloid cell nuclear differentiation antigen (MNDA) is a myelomonocytic lineage-specific protein that influences gene expression through interactions with other nuclear proteins and transcription factors. MNDA also self-associates and chemical cross-linking was used to demonstrate that MNDA forms a dimer. C-terminal and internal deletion mutants were used to identify two regions in the N-terminal half of MNDA essential for self-association. One region contains an imperfect leucine zipper and the second is highly enriched in basic residues. The sequences that are essential for dimerization are separated by a highly basic amphipathic alpha-helical region which was not required for dimerization.
Xie
J
J
Department of Pathology, Vanderbilt University Medical School, Nashville, TN 37232-5310, USA.
Briggs
J A
JA
Briggs
R C
RC
D000595:Amino Acid Sequence; D015214:Antigens, Differentiation, Myelomonocytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D003432:Cross-Linking Reagents / Q000378:metabolism; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006801:Humans; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017384:Sequence Deletion; D013388:Succinimides / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D015214:Antigens, Differentiation, Myelomonocytic; D003432:Cross-Linking Reagents; C075373:MNDA protein, human; D011994:Recombinant Proteins; D013388:Succinimides; D014157:Transcription Factors; C096464:disuccinimidyl glutarate