DNA binding and cleavage by a cationic manganese porphyrin-peptide nucleic acid conjugate.
A cationic manganese porphyrin-peptide nucleic acid (PNA) conjugate has been prepared and used to cleave a double-stranded DNA target. Cleavage experiments were performed with a 247-base pair restriction DNA fragment containing a 10-base pair homopurine binding target for the PNA. Oxidative activation by this Mn porphyrin-PNA conjugate leads to sequence specific, 3'-staggered cleavage of both DNA strands near the strand displacement junction. Furthermore, the Mn porphyrin-PNA porphyrin conjugates bind over 100-fold better to double-stranded DNA compared to the native PNA.
Bigey
P
P
Laboratoire de Chimie de Coordination du CNRS, Toulouse, France.
Sönnichsen
S H
SH
Meunier
B
B
Nielsen
P E
PE
D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D010084:Oxidation-Reduction; D010455:Peptides / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism*
D010455:Peptides; D011166:Porphyrins; D008345:Manganese; D004247:DNA