Human amebic liver abscess: expression of intercellular adhesion molecules 1 and 2 and of von Willebrand factor in endothelial cells.
Liver invasion by amebas with production of amebic liver abscess (ALA) is the most common extraintestinal lesion produced by the protozoan parasite Entamoeba histolytica. This hepatic damage is characterized by the presence of extensive tissue necrosis. However, little is known about the parasite and host factors involved in the process of tissue damage. During the early establishment of amebas in the liver parenchyma as well as during the extension of the tissue necrosis, parasites interact with sinusoidal endothelial cells. As a consequence of ameba-endothelial cell interactions, the latter can be activated and express proinflammatory factors that could be related to tissue destruction. We studied by immunohistochemistry the localization of antigenic molecules of E. histolytica trophozoites and of molecules such as intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and von willebrand factor in activated endothelial cells of human ALA, which could be related to the pathophysiological mechanisms of tissue destruction in amebiasis.
Ventura-Juárez
J
J
Department of Experimental Pathology, Center for Research and Advanced Studies, National Polytechnic Institute, Mexico, D.F.
Campos-Rodríguez
R
R
Rodríguez-Martínez
H A
HA
Rodríguez-Reyes
A
A
Martínez-Palomo
A
A
Tsutsumi
V
V
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D000953:Antigens, Protozoan / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004727:Endothelium / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D004730:Endothelium, Vascular / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D004748:Entamoeba histolytica / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D008099:Liver / Q000276:immunology* / Q000473:pathology; D008101:Liver Abscess, Amebic / Q000276:immunology / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014841:von Willebrand Factor / Q000032:analysis*
D015703:Antigens, CD; D000953:Antigens, Protozoan; D015815:Cell Adhesion Molecules; C065289:ICAM2 protein, human; D014841:von Willebrand Factor; D018799:Intercellular Adhesion Molecule-1