Synoviocyte proliferation in joints of SCID mice induced by toxic shock syndrome toxin-1 stimulated T cells from patient with rheumatoid arthritis.
OBJECTIVE
To investigate the histopathological arthropathy in severe combined immunodeficient (SCID) mice given intraarticular injection of toxic shock syndrome toxin-1 (TSST-1) stimulated T cells from patients with rheumatoid arthritis (RA).


METHODS
Unstimulated or TSST-1 stimulated T cell blasts (TB-TSST) of synovial fluid mononuclear cells from patients with RA (RASFMC) were intraarticularly injected into the knee joint of SCID mice. Four weeks later, the knee joints were histopathologically examined and the numbers of fibroblasts in the synovial tissues were compared with those of controls. Total RNA of the SCID mouse knee joints was isolated and Southern analysis for human T cell receptor (TCR) V beta 2 and human tumor necrosis factor-alpha (TNF-alpha) was carried out.


RESULTS
Hyperplasia and increased numbers of the fibroblasts as well as neovascularization of the synovial tissues were observed in the SCID mouse knee joint tissues injected with TB-TSST of RASFMC compared with those injected with unstimulated T cells from RASFMC or with TB-TSST from peripheral blood of healthy controls. Messenger RNA for human TCR V beta 2 and TNF-alpha were detected in the SCID mouse knee joint tissues injected with TB-TSST from RASFMC.


CONCLUSIONS
Superantigen TSST-1 stimulated T cells from RASFMC have the ability to induce chronic arthropathy with fibroblast proliferation and neovascularization in the SCID mouse.
Suzuki
T
T
Department of Intenal Medicine II, Fukushima Medical College, Japan.
Nishimaki
T
T
Kogure
A
A
Okubo
M
M
Akatsuka
H
H
Kokubun
M
M
Kasukawa
R
R
Natsume
T
T
Suzuki
T
T
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001427:Bacterial Toxins; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D007719:Knee Joint / Q000473:pathology; D008213:Lymphocyte Activation; D051379:Mice; D016513:Mice, SCID; D018089:Superantigens; D013582:Synovial Fluid / Q000166:cytology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000473:pathology
D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal