Allergen-induced recruitment of Fc epsilon RI+ eosinophils in human atopic skin.
We have attempted to identify Fc epsilon RI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30% expressed mRNA for the alpha, beta, and gamma chains of Fc epsilon RI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-alpha chain antibody, the Fc epsilon RI alpha protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of Fc epsilon RI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express Fc epsilon. RI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.
Barata
L T
LT
Allergy and Clinical Immunology, Imperial College School of Medicine at the National Heart & Lung Institute, London, GB.
Ying
S
S
Grant
J A
JA
Humbert
M
M
Barkans
J
J
Meng
Q
Q
Durham
S R
SR
Kay
A B
AB
D000485:Allergens / Q000502:physiology*; D002465:Cell Movement / Q000276:immunology*; D004396:Coloring Agents; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D004804:Eosinophils / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009282:Naphthalenesulfonates; D017455:Receptors, IgE / Q000032:analysis*; D013194:Staining and Labeling
D000485:Allergens; D004396:Coloring Agents; D009282:Naphthalenesulfonates; D017455:Receptors, IgE; C070620:chromotrope 2R