title: Suppression of neutral cholesterol ester hydrolase activity by antisense DNA of hormone-sensitive lipase.
abstract: In order to investigate the role of the hormone-sensitive lipase (HSL) gene in the activity of neutral cholesterol ester hydrolase (NCEH) from a molecular perspective, Chinese hamster ovary (CHO) cells were transfected with rat antisense hormone-sensitive lipase cDNA, and three different cell lines, designated as anti-HSL, were established. NCEH activity in anti-HSL cells was reduced to approximately 50% of that in control CHO cells. The concentration of cellular esterified cholesterol increased and the concentration of free cholesterol decreased in the anti-HSL cell lines. These results suggest that the HSL gene has a function of NCEH as well.
authors.affiliation: Third Department of Internal Medicine, University of Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Osuga
authors.forename: S
authors.initials: S
authors.lastname: Ishibashi
authors.forename: H
authors.initials: H
authors.lastname: Shimano
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: M
authors.initials: M
authors.lastname: Kawamura
authors.forename: Y
authors.initials: Y
authors.lastname: Yazaki
authors.forename: N
authors.initials: N
authors.lastname: Yamada
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics; D004951:Esterification; D005822:Genetic Vectors; D051381:Rats; D002787:Sterol Esterase / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D002788:Cholesterol Esters; D017931:DNA Primers; D016373:DNA, Antisense; D018076:DNA, Complementary; D002784:Cholesterol; D002787:Sterol Esterase