A method for separation and staining of flat mounts of human corneal endothelium.
A technique is described in which sheets of corneal endothelium are removed from human donor corneo-scleral discs. Celloidin solution was applied to the endothelial surface, allowed to dry, peeled off with the attached endothelial cell layer and mounted on a glass slide. Following removal of the celloidin with acetone, this endothelial cell flat mount was then stained with H&E and monoclonal antibodies to cell adhesion molecules. A pilot study of endothelial cell adhesion molecule expression in flat mount preparations of 14 corneas showed constitutive neural cell adhesion molecule (NCAM) expression, but a lower degree of focal expression of intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VACM)-1, P/E-selectin and HLA-DR.
Claesson
M
M
Ogonkliniken, Sahlgrenska sjukhuset, Göteborg, Sweden.
Elder
M J
MJ
Larkin
D F
DF
D002469:Cell Separation / Q000379:methods*; D046508:Culture Techniques; D004728:Endothelium, Corneal / Q000166:cytology* / Q000378:metabolism; D005125:Eye Banks; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D010865:Pilot Projects; D019087:Selectins / Q000378:metabolism; D013048:Specimen Handling; D013194:Staining and Labeling / Q000379:methods; D014021:Tissue Preservation; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D006684:HLA-DR Antigens; D019006:Neural Cell Adhesion Molecules; D019087:Selectins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1