Protein-tyrosine phosphatase activity of Coxiella burnetii that inhibits human neutrophils.
Supernatants prepared from disrupted Coxiella burnetii possess acid phosphatase (ACP) activity that apparently accounts for the inhibition of the metabolic burst of formyl-Met-Leu-Phe(fMLP)-stimulated human neutrophils. Results are presented regarding purification and biochemical-biological characterization of the ACP. The highly purified enzyme, which exhibited an apparent M(r) of 91 K and optimal activity at pH 5.0, also inhibited neutrophils. The enzyme retained full activity at pH 4.5, 5.5, and 7.4, when incubated overnight at 0 degrees C and room temperature; at pH 5.5, it retained full activity after overnight incubation at 37 degrees C. Apparently, the enzyme contains asparagine-linked but not serine- or threonine-linked glycan residues since its treatment with N-glycosidase F (PNGase F) decreased its M(r) to 87 K and no changes were detected with O-glycosidase. The enzyme's capacity to hydrolyze phosphate from a number of phosphate-containing compounds was examined; five phosphocompounds were significantly hydrolyzed: 5'-CMP > fructose 1,6-diphosphate > tyrosine phosphate > 3'-AMP > 5'-AMP. The ACP also dephosphorylated (32)P-Raytide, a phosphotyrosine-containing peptide. Dephosphorylation of Raytide was inhibited by the following phosphatase inhibitors: sodium molybdate, potassium fluoride, sodium ortho-vanadate and D2, a heteropolymolybdate compound. These results indicate that C. burnetii ACP may play a role in disrupting tyrosine phosphorylation/dephosphorylation reactions associated with the signal transduction pathway culminating in the metabolic burst. Interestingly, Western blot analysis of ACP-inhibited neutrophils showed a marked increase in tyrosine phosphorylation of a 44 K protein as compared to uninhibited cells.
Li
Y P
YP
Biology Department, University of New Mexico, Albuquerque 87131-1091, USA.
Curley
G
G
Lopez
M
M
Chavez
M
M
Glew
R
R
Aragon
A
A
Kumar
H
H
Baca
O G
OG
D000135:Acid Phosphatase / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D000963:Antimetabolites / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D016997:Coxiella burnetii / Q000201:enzymology*; D017886:Durapatite; D004795:Enzyme Stability; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007739:L Cells; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D013696:Temperature; D014443:Tyrosine; D014638:Vanadates
D000963:Antimetabolites; D001426:Bacterial Proteins; D014638:Vanadates; D014443:Tyrosine; D017886:Durapatite; D000135:Acid Phosphatase; D017027:Protein Tyrosine Phosphatases; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase