title: Epstein-Barr virus and human herpesvirus 8 prevalence in human immunodeficiency virus-associated oral mucosal lesions.
abstract: The prevalence of Epstein-Barr virus (EBV) and the recently identified Kaposi's sarcoma (KS)-associated herpesvirus (also designated human herpesvirus 8 [HHV-8]) was determined in oral lesions and oral neoplasms common to persons with human immunodeficiency virus (HIV) infection. Oral lesions were examined by polymerase chain reaction (PCR) for EBV and HHV-8 DNA and by Southern blot analysis for EBV clonality. EBV was detected by Southern blot in hairy leukoplakia lesions, in a subset of AIDS-related lymphomas, and in saliva from HIV-positive persons but not in pseudohairy leukoplakia lesions, oral aphthous ulcers, or oral KS lesions. EBV was detected, however, by PCR in most of the lesions, while HHV-8 was detected only in oral KSs. The absence of HHV-8 DNA in both the EBV-associated hairy leukoplakia lesions and in the EBV-associated AIDS-related lymphomas strengthens the etiologic relationship of EBV to these pathologies and the etiologic role of HHV-8 in KS.
authors.affiliation: Department of Microbiology and Immunology and Lineberger Cancer Center, University of North Carolina at Chapel Hill, 27599-7295, USA.
authors.forename: J
authors.initials: J
authors.lastname: Webster-Cyriaque
authors.forename: R H
authors.initials: RH
authors.lastname: Edwards
authors.forename: E B
authors.initials: EB
authors.lastname: Quinlivan
authors.forename: L
authors.initials: L
authors.lastname: Patton
authors.forename: D
authors.initials: D
authors.lastname: Wohl
authors.forename: N
authors.initials: N
authors.lastname: Raab-Traub
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000821:virology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D006566:Herpesviridae Infections / Q000150:complications / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017733:Leukoplakia, Hairy / Q000821:virology; D016483:Lymphoma, AIDS-Related / Q000821:virology; D008297:Male; D009059:Mouth Diseases / Q000150:complications / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012514:Sarcoma, Kaposi / Q000821:virology; D017422:Sequence Analysis, DNA; D014412:Tumor Virus Infections / Q000150:complications / Q000821:virology
chemical_list: D004279:DNA, Viral