title: Nucleotide sequence analysis of the HLA class I region spanning the 237-kb segment around the HLA-B and -C genes.
abstract: To elucidate the detailed gene organization of the human leukocyte antigen (HLA) class I region on chromosome 6, seven contiguous cosmid genomic clones covering the 237-kb segment around the HLA-B and -C loci were subjected to DNA sequencing by the shotgun strategy to give a single contig of 236,822 bp from the MICA gene (58.2 kb centromeric of HLA-B) to 90.8 kb telomeric of HLA-C. This region was confirmed to contain four known genes, MICA, HLA-17, HLA-B, and HLA-C, from centromere to telomere. Further, a new member of the P5 multicopy genes was found to be about 1.3 kb upstream of the HLA-17 gene and designated P5.8. Five novel genes designated NOB1-5 were identified by RT-PCR and Northern blot hybridization. In addition, two pseudogenes, dihydrofolate reductase pseudogene (DHFRP) and ribosomal protein L3 homologous gene (RPL3-Hom), were also found in the vicinity of the HLA-B and -C genes, respectively. The two segments (about 40 kb) downstream of the HLA-B and HLA-C genes showed high sequence homology to each other, suggesting that segmental genome duplication including the major histocompatibility complex (MHC) class I gene must have occurred during the evolution of the MHC.
authors.affiliation: Department of Genetic Information, Tokai University School of Medicine, Kanagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Mizuki
authors.forename: H
authors.initials: H
authors.lastname: Ando
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Ohno
authors.forename: S
authors.initials: S
authors.lastname: Miyata
authors.forename: M
authors.initials: M
authors.lastname: Yamazaki
authors.forename: H
authors.initials: H
authors.lastname: Tashiro
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: A
authors.initials: A
authors.lastname: Ono
authors.forename: S
authors.initials: S
authors.lastname: Taguchi
authors.forename: C
authors.initials: C
authors.lastname: Sugawara
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuzumi
authors.forename: K
authors.initials: K
authors.lastname: Okumura
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: M
authors.initials: M
authors.lastname: Ishihara
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: J
authors.initials: J
authors.lastname: Yonemoto
authors.forename: Y Y
authors.initials: YY
authors.lastname: Kikuti
authors.forename: T
authors.initials: T
authors.lastname: Shiina
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Ando
authors.forename: T
authors.initials: T
authors.lastname: Ikemura
authors.forename: H
authors.initials: H
authors.lastname: Inoko
mesh_terms: D015152:Blotting, Northern; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000235:genetics; D019143:Evolution, Molecular; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D000090822:Ribosomal Protein L3; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
chemical_list: D015235:HLA-B Antigens; D015236:HLA-C Antigens; D012333:RNA, Messenger; C000716547:RPL3 protein, human; D000090822:Ribosomal Protein L3; D004247:DNA