Variation in plasma fibrinogen over one year: relationships with genetic polymorphisms and non-genetic factors.
We analyzed plasma fibrinogen level in relation to genetic polymorphisms in the alpha- and beta-fibrinogen gene loci. Furthermore, the association of other CVD risk markers with fibrinogen was studied twice, with a time interval of one year in 50 to 60 year old men (n = 183). DNA polymorphisms were detected by PCR and digestion with Taq I (alpha-fibrinogen), Hind III and Bcl I (beta-fibrinogen) restriction enzymes. The correlation coefficient between fibrinogen measurements was 0.48 (p < 0.001). Blood leucocytes and waist-to-hip circumference ratio were the strongest correlates of fibrinogen level in both examinations, and the changes in leucocyte count and plasma fibrinogen correlated positively (r = 0.40, p < 0.001). In Eastern Finnish men, the Taq I, Hind III or Bcl I restriction fragment length polymorphisms of the alpha- or beta-fibrinogen gene loci did not associate with fibrinogen level, either cross-sectionally or longitudinally.
Väisänen
S
S
Kuopio Research Institute of Exercise Medicine, University of Kuopio, Finland.
Rauramaa
R
R
Penttilä
I
I
Rankinen
T
T
Gagnon
J
J
Pérusse
L
L
Chagnon
M
M
Bouchard
C
C
D015415:Biomarkers; D001824:Body Constitution; D008076:Cholesterol, HDL / Q000097:blood; D015331:Cohort Studies; D003327:Coronary Disease / Q000453:epidemiology; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004734:Energy Metabolism; D005340:Fibrinogen / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D005387:Finland; D006801:Humans; D007958:Leukocyte Count; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D010101:Oxygen Consumption; D012150:Polymorphism, Restriction Fragment Length; D011897:Random Allocation; D012307:Risk Factors; D013997:Time Factors
D015415:Biomarkers; D008076:Cholesterol, HDL; D005340:Fibrinogen; D015247:Deoxyribonuclease HindIII; C041666:endodeoxyribonuclease BclI; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases