Isoenzymes of Eimeria from the domestic fowl: electrophoretic variants among species, strains and clones.
Electrophoretic variation of enzymes in five Eimeria spp. of the domestic fowl, including nine strains, ten single-sporocyst clones and two single-sporozoite clones of E. acervulina, three strains each of E. maxima and E. tenella, two strains of E. praecox and one strain of E. necatrix, were assayed using cellulose acetate electrophoresis. Ten enzymes [aldehyde oxidase (AO), alkaline phosphatase (ALP), amylase (AMY), fumarate hydratase (FUM), glucose-6-phosphate dehydrogenase (G6PDH), glucose phosphate isomerase (GPI), glutamate-oxaloacetate transferase (GOT), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH) and phosphoglucomutase (PGM)] were analyzed for their ability to distinguish between these species and strains. Enzymatic activity of G6PDH, GPI, IDH, MDH and PGM was detected in all the Eimeria spp. examined. Strains within each species were characterized by the same electrophoretic variant of G6PDH. Electrophoretic variants of GPI and PGM were the most valuable in the identification of inter- and intra-specific variation, particularly in the field strains of E. acervulina and E. tenella. These two enzymes were used to examine single-sporocyst and single-sporozoite clones derived from two strains of E. acervulina. The enzymes in E. maxima appeared to be conserved, showing no variation among strains with the five enzymes detected. Relative mobilities, calculated as described in this paper, were found to be consistent between different electrophoresis runs and may serve as a reference when this medium is used.
Johnston
D A
DA
Dept. of Pathobiology, University of Guelph, Ontario, Canada.
Fernando
M A
MA
D000818:Animals; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000201:enzymology*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D007521:Isocitrate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis; D010733:Phosphoglucomutase / Q000032:analysis; D011201:Poultry Diseases / Q000469:parasitology*
D007527:Isoenzymes; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase