Cloning and characterization of Gu/RH-II binding protein.
Gu/RNA helicase II (Gu/RH-II) is the first reported mammalian nucleolar RNA helicase that is a member of the D-E-A-D (Asp-Glu-Ala-Asp) box family of proteins. It has an ATP-dependent RNA unwinding (helicase) activity and a separate RNA folding activity (introduction of intramolecular secondary structure into single-stranded RNA). To determine which proteins may bind to Gu/RH-II, a yeast two-hybrid system was used. A cDNA which encoded a protein, called Gu/RH-II binding protein or GBP, was isolated and sequenced. The GBP protein is localized to the nucleus in speckled or diffuse nucleoplasmic patterns. The GBP mRNA level is highest in testis, 9- to 49-fold greater than other tissues. When GBP interacts with Gu/RH-II, proteolytic cleavage of Gu/RH-II occurs; the amino-terminal portion of Gu/RH-II is critical for this proteolysis.
Valdez
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Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA. bvaldez@bcm.tmc.edu
Henning
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Perlaky
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Busch
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Busch
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D050828:Protein Inhibitors of Activated STAT; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D025841:Small Ubiquitin-Related Modifier Proteins; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution; D014162:Transfection
D002352:Carrier Proteins; D018076:DNA, Complementary; C106529:PIAS1 protein, human; D050828:Protein Inhibitors of Activated STAT; D012333:RNA, Messenger; D025841:Small Ubiquitin-Related Modifier Proteins; D012316:RNA Nucleotidyltransferases; D020365:RNA Helicases