Human synaptotagmin V (SYT5): sequence, genomic structure, and chromosomal location.
We have determined the sequence, genomic structure, and chromosomal location of the human synaptotagmin V (SYTV) gene. The human SYTV gene encodes a 386-amino-acid product which is 91% identical to rat Syt V. The human SYTV open reading frame is interrupted by seven introns which can be alternatively spliced. Human SYTV was found to lie very close to SYTIII on chromosome 19q13.4 by PCR analysis of somatic cell hybrid DNA and by DNA hybridization to arrayed cosmids of the chromosome 19 metric physical map. This provides the first report of linked synaptotagmin genes.
Craxton
M
M
Medical Research Council, Laboratory of Molecular Biology, Cambridge, United Kingdom.
Olsen
A
A
Goedert
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D016384:Consensus Sequence; D003360:Cosmids; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D007438:Introns; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D050857:Synaptotagmins; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C494295:SYT5 protein, human; C494297:Syt5 protein, rat; D050857:Synaptotagmins; D004247:DNA