A novel culture morphology resulting from applied mechanical strain.
To demonstrate that cells both perceive and respond to external force, a strain/relaxation regimen was applied to normal human fetal and aged dermal fibroblasts cultured as monolayers on flexible membranes. The precisely controlled protocol of stretch (20% elongation of the culture membrane) at 6.67 cycles/min caused a progressive change in the monolayers, such that the original randomly distributed pattern of cells became a symmetric, radial distribution as the cell bodies aligned parallel to the applied force. High cell density interfered with the success of re-alignment in the fetal cell cultures observed, which may reflect a preference in this cell strain for cell-cell over cell-matrix contacts. The chronologically aged cells observed did not demonstrate this feature, aligning efficiently at all seeding densities examined. The role of microfilaments in force perception and transmission was investigated through the addition of cytochalasin D in graded doses. Both intercellular interactions and cytoskeletal integrity mediate the morphological response to mechanical strain.
Grymes
R A
RA
Cell and Molecular Biology Laboratory, NASA-Ames Research Center and Bionetics Corporation, Moffett Field, California 94035, USA.
Sawyer
C
C
D000199:Actins / Q000502:physiology; D001696:Biomechanical Phenomena; D002452:Cell Count; D048429:Cell Size; D002478:Cells, Cultured; D016922:Cellular Senescence; D003470:Culture Media / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D010812:Physical Stimulation
D000199:Actins; D003470:Culture Media; D015638:Cytochalasin D