title: Demonstration of common and stage-specific anti-Hepatozoon mocassini antibodies in experimentally infected unnatural lizard hosts.
abstract: This study examines the immunological response to Hepatozoon mocassini in lizards. Three lizard species were infected experimentally with H. mocassini. Baseline and post-infection (PI) sera were assayed for anti-H. mocassini meront and gametocyte antibody by immunohistochemistry and IFA. Seroconversion occurred at 38 d PI with endpoint IFA titers of 1:64. Antisera from non-parasitaemic and parisitaemic lizards exhibited similar affinities for merozite and gametocyte antigens. Antibody specific for the membranes of gametocyte-infected erythrocytes was demonstrated exclusively in parasitaemic lizards. The results demonstrate that lizards infected with snake haemogregarines mount an antibody response with common and stage-specific components.
authors.affiliation: Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Wozniak
authors.forename: C
authors.initials: C
authors.lastname: Kanitz
authors.forename: B
authors.initials: B
authors.lastname: Homer
authors.forename: R
authors.initials: R
authors.lastname: Kreisle
authors.forename: S R
authors.initials: SR
authors.lastname: Telford
authors.forename: G
authors.initials: G
authors.lastname: McLaughlin
mesh_terms: D000330:Aedes / Q000469:parasitology; D017836:Agkistrodon / Q000469:parasitology; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D016783:Eucoccidiida / Q000276:immunology*; D007150:Immunohistochemistry; D008116:Lizards / Q000276:immunology* / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D018512:Parasitemia / Q000276:immunology; D013045:Species Specificity
chemical_list: D000913:Antibodies, Protozoan