NGF and BDNF increase the immunoreactivity of vesicular acetylcholine transporter in cultured neurons from the embryonic rat septum.
The expression of vesicular acetylcholine transporter (VAChT), which transports ACh into synaptic vesicles, is coregulated with choline acetyltransferase (ChAT). Therefore, the effects of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) on the levels of VAChT in cultured neurons from the septum of embryonic rats were investigated by immunocytochemistry. NGF and BDNF increased the number of VAChT-immunoreactive neurons by approximately 1.5-fold and enhanced the immunoreactivity in each positive cell. These results suggest that the neurotrophins enhance not only synthesis but also storage of ACh in septal neurons.
Takei
N
N
Department of Applied Biology, Kyoto Institute of Technology, Sakyo, Japan. nobu.takei@mun.uu.se
Kuramoto
H
H
Endo
Y
Y
Hatanaka
H
H
D000109:Acetylcholine; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002352:Carrier Proteins / Q000032:analysis*; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D007150:Immunohistochemistry; D026901:Membrane Transport Proteins; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D012688:Septum Pellucidum / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
D019208:Brain-Derived Neurotrophic Factor; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D009414:Nerve Growth Factors; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D000109:Acetylcholine