title: Use of virginiamycin to control the growth of lactic acid bacteria during alcohol fermentation.
abstract: The antibiotic virginiamycin was investigated for its effects on growth and lactic acid production by seven strains of lactobacilli during the alcoholic fermentation of wheat mash by yeast. The lowest concentration of virginiamycin tested (0.5 mg Lactrol kg-1 mash), was effective against most of the lactic acid bacteria under study, but Lactobacillus plantarum was not significantly inhibited at this concentration. The use of virginiamycin prevented or reduced potential yield losses of up to 11% of the produced ethanol due to the growth and metabolism of lactobacilli. However, when the same concentration of virginiamycin was added to mash not inoculated with yeast, Lactobacillus rhamnosus and L. paracasei grew after an extensive lag of 48 h and L. plantarum grew after a similar lag even in the presence of 2 mg virginiamycin kg-1 mash. Results showed a variation in sensitivity to virginiamycin between the different strains tested and also a possible reduction in effectiveness of virginiamycin over prolonged incubation in wheat mash, especially in the absence of yeast.
authors.affiliation: Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon, Canada.
authors.forename: S H
authors.initials: SH
authors.lastname: Hynes
authors.forename: D M
authors.initials: DM
authors.lastname: Kjarsgaard
authors.forename: K C
authors.initials: KC
authors.lastname: Thomas
authors.forename: W M
authors.initials: WM
authors.lastname: Ingledew
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D019344:Lactic Acid / Q000096:biosynthesis; D007778:Lactobacillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014908:Triticum / Q000378:metabolism; D014769:Virginiamycin / Q000494:pharmacology*; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D014769:Virginiamycin; D019344:Lactic Acid; D000431:Ethanol