title: Effects of mild hypothermia on nitric oxide synthesis following contusion trauma in the rat.
abstract: The exact mechanism of hypothermic cerebroprotection after traumatic brain injury (TBI) is not fully understood. The present study was conducted to investigate the effects of mild hypothermia on trauma-induced synthesis of nitric oxide (NO), which has been implicated in the pathogenesis of ischemic brain damage associated with glutamate neurotoxicity. Cerebral contusion was created in the rat parietal cortex by a weight-drop method, and extracellular concentrations of the NO end products nitrite and nitrate were measured using in vivo brain microdialysis and capillary electrophoresis under normothermic (37 degrees C) and mild hypothermic (32 degrees C) conditions. In normothermic animals, the level of NO end products increased markedly 10 min after contusion, reaching a maximum level at 20 min. In the hypothermic rats, such increases were absent. Although it is unknown whether endothelial NO synthase, neuronal NO synthase, or both caused the elevation of the NO end products seen in the normothermic animals, the present results indicate that inhibition of NO synthesis may play a part in hypothermic cerebroprotection following TBI.
authors.affiliation: Department of Neurosurgery, Yamaguchi University School of Medicine, Kogushi, Ube, Japan.
authors.forename: K I
authors.initials: KI
authors.lastname: Sakamoto
authors.forename: H
authors.initials: H
authors.lastname: Fujisawa
authors.forename: H
authors.initials: H
authors.lastname: Koizumi
authors.forename: E
authors.initials: E
authors.lastname: Tsuchida
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.forename: D
authors.initials: D
authors.lastname: Sadamitsu
authors.forename: T
authors.initials: T
authors.lastname: Maekawa
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism / Q000628:therapy*; D003288:Contusions / Q000378:metabolism*; D007035:Hypothermia / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019001:Nitric Oxide Synthase