title: ARF1, a transcription factor that binds to auxin response elements.
abstract: The plant hormone auxin regulates plant physiology by modulating the interaction of transcription factors with auxin response elements (AuxREs) of the affected genes. A transcription factor, Auxin Response Factor 1 (ARF1), that binds to the sequence TGTCTC in AuxREs was cloned from Arabidopsis by using a yeast one-hybrid system. ARF1 has an amino-terminal DNA-binding domain related to the carboxyl terminus of the maize transactivator Viviparous-1. Sequence requirements for ARF1 binding in vitro are identical to those that confer auxin responsiveness in vivo. The carboxyl terminus of ARF1 contains two motifs found in the Aux/IAA class of proteins and appears to mediate protein-protein interactions.
authors.affiliation: Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, MO 65211, USA.
authors.forename: T
authors.initials: T
authors.lastname: Ulmasov
authors.forename: G
authors.initials: G
authors.lastname: Hagen
authors.forename: T J
authors.initials: TJ
authors.lastname: Guilfoyle
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C503841:ARF1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D007210:Indoleacetic Acids; D010940:Plant Proteins; D014157:Transcription Factors