Limitation of Hu-PBL-scid mouse model in direct application to immunotoxicity assessment.
Hu-PBL-scid mice were directly introduced to the methods of immunotoxicity assessments. Human IgG and IgM was detected 1 week after transplantation. Cyclosporin A (CsA) and cyclophosphamide (CP), which were injected i.p. 4 weeks after transplantation, decreased the serum concentration of IgM after 2-4 days of treatment but not that of IgG. Lymphocyte proliferation induced by various mitogens and primary T-dependent antibody responses to sheep red blood cells could not be measured by using splenocytes of hu-PBL-scid mice. These results were correlated with the fact that human cells were not detected in the spleen, thymus, or blood of hu-PBL-scid mouse but were detected in lymph nodes of the intestine, which were observed by flow cytometric and immunohistochemical examinations. The present results suggest using hu-PBL-scid mice in routine immunotoxicity investigations: lymph nodes of intestines could be used as the lymphocyte source. In addition, the determination of serum Ig concentration might be used as a experimental item.
Kim
H M
HM
Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon, Korea.
Han
S B
SB
Hong
D H
DH
Yoo
B S
BS
Oh
G T
GT
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002455:Cell Division; D003520:Cyclophosphamide / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D017710:Lymphocyte Transfusion; D051379:Mice; D016513:Mice, SCID; D013154:Spleen / Q000166:cytology
D007136:Immunoglobulins; D007166:Immunosuppressive Agents; D016572:Cyclosporine; D003520:Cyclophosphamide