An expression vector encoding a lacZ reporter gene facilitates identification of stable, high-producing CHO cell clones.
We describe a vector and a streamlined procedure for isolating high-producing stable mammalian cell transfectants. The vector encodes the Escherichia coli lacZ gene as a reporter. We show that levels of beta-galactosidase activity, assayed in situ in clonal isolates, can be used to identify clones producing high levels of the protein of interest (in this case, soluble human CD4 protein).
Grossman
T H
TH
Department of Molecular Biology, Procept. Inc., Cambridge, Massachusetts, USA.
Godoy
J E
JE
Kawasaki
E S
ES
Osburne
M S
MS
D000818:Animals; D015704:CD4 Antigens / Q000096:biosynthesis / Q000235:genetics; D016466:CHO Cells; D002999:Clone Cells; D006224:Cricetinae; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D007763:Lac Operon; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D015704:CD4 Antigens; D011994:Recombinant Proteins; D001616:beta-Galactosidase