title: A rapid and simple method to quantify Helicobacter pylori adhesion to human gastric MKN-28 cells.
abstract: A rapid and simple method to quantitatively analyse Helicobacter pylori adhesion to human gastric epithelial cells was developed by using an enzyme-linked immunosorbent assay. This method made it possible to quantitatively evaluate the adhesion activities of many different H. pylori strains at a time. Our studies indicate that this method is well suited for the quantitative analysis of H. pylori adhesion to gastric epithelial cells.
authors.affiliation: Department of Microbiology, School of Medicine, Sapporo Medical University, Hokkaido, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
authors.forename: A
authors.initials: A
authors.lastname: Yachi
authors.forename: K
authors.initials: K
authors.lastname: Yokota
authors.forename: Y
authors.initials: Y
authors.lastname: Hirai
authors.forename: K
authors.initials: K
authors.lastname: Oguma
authors.forename: N
authors.initials: N
authors.lastname: Fujii
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000378:metabolism*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured