title: Identification of a cis-acting element within the herpesvirus saimiri ORF 6 promoter that is responsive to the HVS.R transactivator.
abstract: We have previously demonstrated that two distinct transcripts are produced from ORF 50, the major transcriptional activating gene of herpesvirus saimiri. The products of these transcripts trans-activate the delayed-early ORF 6 promoter, though to different degrees. Deletion analysis demonstrated that the ORF 50 responsive elements are contained in a 132 bp fragment situated 127-259 bp from the transcription initiation site within the ORF 6 promoter. This fragment conferred ORF 50-responsiveness on an enhancerless simian virus 40. Gel retardation analysis further mapped the responsive elements to a 38 bp fragment.
authors.affiliation: Molecular Medicine Unit, University of Leeds, St James's University Hospital, UK.
authors.forename: A
authors.initials: A
authors.lastname: Whitehouse
authors.forename: A J
authors.initials: AJ
authors.lastname: Stevenson
authors.forename: M
authors.initials: M
authors.lastname: Cooper
authors.forename: D M
authors.initials: DM
authors.lastname: Meredith
mesh_terms: D001483:Base Sequence; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation