Purification and crystallisation of the autoantigen thyroid peroxidase from human Graves' thyroid tissue.
Milligram quantities of the human membrane autoantigen thyroid peroxidase (TPO) have been purified to a high degree of homogeneity by a combination of detergent solubilisation, monoclonal antibody affinity, and ion exchange chromatography, from pooled Graves' disease thyroid glands. The purified TPO of greater than 90% purity was enzymatically active as judged by its ability to oxidise guaiacol. Crystals of TPO have been grown from solutions of the protein solubilised in sodium deoxycholate, in the presence of ammonium sulphate. The crystals exhibited birefringence under polarised light, indicative of molecular order. Crystallisation of this large, membrane autoantigen represents the first step in delineating the complete three-dimensional structure of a human autoantigen involved in destructive thyroiditis.
Gardas
A
A
Medical Centre of Postgraduate Education, Warsaw, Poland.
Sohi
M K
MK
Sutton
B J
BJ
McGregor
A M
AM
Banga
J P
JP
D001324:Autoantigens / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D003840:Deoxycholic Acid; D006111:Graves Disease / Q000201:enzymology* / Q000276:immunology*; D006139:Guaiacol; D006801:Humans; D007453:Iodide Peroxidase / Q000276:immunology* / Q000302:isolation & purification*; D012995:Solubility; D013379:Substrate Specificity; D013961:Thyroid Gland / Q000201:enzymology* / Q000276:immunology*
D001324:Autoantigens; D003840:Deoxycholic Acid; D006139:Guaiacol; D007453:Iodide Peroxidase