Novel quantitative trait loci for blood pressure and related traits on rat chromosomes 1, 10, and 18.
Hypertension and diabetes mellitus are known to be frequently associated. The genetic dissection of diseases such as hypertension or diabetes mellitus is possible by using experimental crosses, which allow identification of loci influencing phenotypic traits (quantitative trait loci - QTLs). In this study the spontaneously hypertensive rat (SHR) and spontaneously diabetic, but normotensive rat (BB/OK) were crossed and the F2 population was analysed in order to search for QTLs on selected chromosomes (1, 10, 18) for blood pressure and some metabolic traits related to diabetes, renal function and hypertension. There were 3 regions found on chromosome 1 which showed linkage to blood pressure. The strongest evidence for linkage was observed between loci Igf2 and D1Mgh12. On chromosome 10 there was a QTL for blood pressure found between Ppy and Abp and on chromosome 18 there were three regions (Ttr-Grl, Tilp-Gja1, Olf-D18Mit9) with linkage to blood pressure. Since the 24 hr albumin and phosphate excretion correlated with blood pressure in F2 hybrids, the same regions were linked to both parameters. Region with linkage to serum concentrations of cholesterol (probably located beyond the terminal marker Ttr of the linkage group) were also found. The results of this study with a new F2(BB x SHR) population confirm the existence of previously described blood pressure loci (Sa and Bp2) and showed novel QTLs on chromosomes 1, 10 and 18.
Kovács
P
P
Department of Laboratory Animal Sciences, Institute of Pathophysiology, University of Greifswald, Karlsburg, Germany.
Voigt
B
B
Klöting
I
I
D000419:Albuminuria; D000483:Alleles; D000818:Animals; D001794:Blood Pressure / Q000235:genetics*; D001798:Blood Proteins / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D002875:Chromosomes / Q000235:genetics*; D003433:Crosses, Genetic; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D006973:Hypertension / Q000235:genetics; D008126:Lod Score; D008297:Male; D010710:Phosphates / Q000652:urine; D051381:Rats; D011913:Rats, Inbred BB; D011918:Rats, Inbred SHR
D001798:Blood Proteins; D005819:Genetic Markers; D010710:Phosphates; D002784:Cholesterol