Biphasic control of NF-kappa B activation induced by the triggering of HLA-DR antigens expressed on B cells.
The regulation of NF-kappa B activation following the triggering of HLA-DR antigens by mAb L243 has been studied at various times in Raji cells. Electrophoretic mobility shift assays demonstrated a strong increase of NF-kappa B DNA binding after triggering of HLA-DR antigens. Using TNF-alpha-activity neutralizing antibodies, the authors demonstrated that the upregulation of NF-kappa B was found to depend, at later time point, on an autocrine effect of TNF-alpha secreted following triggering of HLA-DR antigens. In contrast, it was found to be TNF-alpha independent in the early time point. Moreover, the upregulation of NF-kappa B binding activity is regulated by the triggering of selected epitopes of HLA-DR antigens. In fact, mAb L243 but not the staphylococcal superantigens, staphylococcal exotoxin toxic shock syndrome toxin-I or staphylococcal enterotoxin B, regulate the NF-kappa B binding activity.
Leonardi
A
A
Department of Sciences and Biomedical Technologies, University of Udine, Italy.
Altomonte
M
M
Maio
M
M
Tell
G
G
Bearz
A
A
Formisano
S
S
Pucillo
C
C
D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit; D011518:Proto-Oncogene Proteins / Q000276:immunology; D020569:Proto-Oncogene Proteins c-rel; D051996:Transcription Factor RelA; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha