title: Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers.
abstract: We used a combination of sequence analysis and exon trapping in an effort to determine the complete transcript map for a cosmid (6E5) derived from 12q13.3, a region of DNA sequence amplification in human cancers. This cosmid, previously known to contain three genes (CDK4, SAS, and OS9), was sequenced, and that information was used for computer-assisted analysis. In addition, 6E5 was subjected to both internal and 3'-terminal exon-trapping protocols, and the results of these studies were used to guide cDNA cloning experiments. These studies demonstrate that this cosmid is derived from a remarkably gene-dense region and add two new transcripts (KIAA0167 and 6E5.2) to the list of sequences that are expressed in tumors bearing amplification of this region.
authors.affiliation: Laboratory of Cancer Genetics, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892-4470, USA.
authors.forename: A G
authors.initials: AG
authors.lastname: Elkahloun
authors.forename: D B
authors.initials: DB
authors.lastname: Krizman
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: T A
authors.initials: TA
authors.lastname: Hofmann
authors.forename: B
authors.initials: B
authors.lastname: Roe
authors.forename: P S
authors.initials: PS
authors.lastname: Meltzer
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D018076:DNA, Complementary / Q000235:genetics; D016208:Databases, Factual; D005091:Exons; D005784:Gene Amplification; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary