Adhesion of HT-29 colon carcinoma cells to E-selectin results in increased tyrosine phosphorylation and decreased activity of c-src.
Adhesion of metastatic cancer cells at secondary sites is known to be regulated by several families of adhesion proteins, including selectins and integrins. Colon carcinoma cells have been shown to tether to and roll on both stimulated endothelial cells and purified E-selectin. We have demonstrated that HT-29 human colon carcinoma cells adhere specifically to an E-selectin-IgG chimera. Upon adhesion to E-selectin, the amount of tyrosine phosphorylation of several proteins in HT-29 cell lysates increases compared with cells in bovine serum albumin-coated wells on phosphotyrosine Western blots; this increase is statistically significant. This effect is specific for adhesion to E-selectin, since addition of an E-selectin blocking monoclonal antibody (MAb), E3, to the wells causes a statistically significant decrease in tyrosine phosphorylation relative to E-selectin alone on phosphotyrosine Western blots. One protein that is affected this way has been identified as c-src. Kinase assays show a dose-dependent and statistically significant decrease in c-src activity upon adhesion to E-selectin, which correlates with an increase in phosphorylation of Tyr 527, the negative regulatory tyrosine. CnBr digestion of 32P-labeled c-src shows an increase in phosphorylation of tyrosine 527 after adhesion to E-selectin. Our results may identify a signaling pathway involving the E-selectin ligand on HT-29 cells and c-src.
Soltesz
S A
SA
Cancer Research, Pharmacia and Upjohn Inc., Kalamazoo, MI 49001, USA.
Powers
E A
EA
Geng
J G
JG
Fisher
C
C
D000818:Animals; D002277:Carcinoma / Q000473:pathology*; D002417:Cattle; D002448:Cell Adhesion; D002465:Cell Movement; D003110:Colonic Neoplasms / Q000473:pathology*; D003470:Culture Media; D019040:E-Selectin / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D012710:Serum Albumin, Bovine; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D003470:Culture Media; D019040:E-Selectin; D009363:Neoplasm Proteins; D012710:Serum Albumin, Bovine; D016392:Proto-Oncogene Proteins pp60(c-src)