Characterization of endonuclease III (nth) and endonuclease VIII (nei) mutants of Escherichia coli K-12.
The nth and nei genes of Escherichia coli affect the production of endonuclease III and endonuclease VIII, respectively, glycosylases/apurinic lyases that attack DNA damaged by oxidizing agents. Here, we provide evidence that oxidative lethal lesions are repaired by both endonuclease III and endonuclease VIII and that spontaneous mutagenic lesions are repaired mainly by endonuclease III.
Saito
Y
Y
Biological Institute, Graduate School of Science, Tohoku University, Sendai, Japan.
Uraki
F
F
Nakajima
S
S
Asaeda
A
A
Ono
K
K
Kubo
K
K
Yamamoto
K
K
D003001:Cloning, Molecular; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation
D029968:Escherichia coli Proteins; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer); C106403:NTH protein, E coli