title: [Study of DNA content and its association with staging, tumor size, architecture, cell type, and nuclear grade in renal adenocarcinoma].
abstract: OBJECTIVE
  To present the results of the analysis of DNA content in renal adenocarcinoma and its correlation with histopathological findings as a first step to conduct predictive studies.
  
  
  METHODS
  DNA analysis was carried out by flow cytometry in deparaffined and fresh samples (1-4 per tumour) from 192 tumours. Correlation to stage, size, growth pattern and grade was studied using the squared chi test.
  
  
  RESULTS
  The percentage of non-diploid tumours increased with the number of samples analyzed. Use of multiple sampling allowed to classify as non-diploid an additional 19% tumours. 57% tumours were homogeneously diploid, while the rest were non-diploid showing a wide variety of patterns: homogeneous, heterogeneous, and even tumours with more than one different non-diploid population. A positive correlation was seen between DNA content pattern, pathological stage and grade.
  
  
  CONCLUSIONS
  Multiple sampling is essential to obtain representative information on DNA content. Prior to conduct predictive studies, the correlation between DNA content, stage and grade should be studied to preclude addition of non-independent information.
authors.affiliation: Servicio de Urología, Hospital Universitario La Fe, Valencia.
authors.forename: J L
authors.initials: JL
authors.lastname: Ruiz Cerdá
authors.forename: M
authors.initials: M
authors.lastname: Hernández Martí
authors.forename: A
authors.initials: A
authors.lastname: Sempere Talens
authors.forename: J
authors.initials: J
authors.lastname: Martínez Jabaloyas
authors.forename: M
authors.initials: M
authors.lastname: Rodrígo Aliada
authors.forename: J L
authors.initials: JL
authors.lastname: Monros Lliso
authors.forename: J F
authors.initials: JF
authors.lastname: Jiménez Cruz
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging
chemical_list: D004273:DNA, Neoplasm