title: Serological indicators of Helicobacter pylori infection in adult dyspeptic patients and healthy blood donors.
abstract: The levels of IgM, IgG and IgA antibodies reacting with two Helicobacter pylori antigens (glycine acid extract (GE) and a recombinant CagA protein) were determined in the sera from adult dyspeptic patients, positive (H.p.(+)) or negative (H.p.(-)) for H. pylori urease/culture, and from healthy blood donors. All sera were also examined against GE by Western blot (Immunoblot) technique. Similar levels of anti-GE IgG were detected in the sera from all H.p.(+) and almost all H.p.(-) patients and from over 40% of the healthy volunteers. In contrast, higher levels of anti-GE IgA were found in the sera from patients than that from healthy subjects, although such antibodies were not detected in the sera from 30% of the H.p.(+) patients. In general, our results suggest that a combination of ELISA and immunoblot may be more sensitive in the detection of H. pylori infection in dyspeptic patients than the examination of biopsy specimens by culturing or histology.
authors.affiliation: K. Jonscher Hospital in Lódź, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Rechciński
authors.forename: M
authors.initials: M
authors.lastname: Chmiela
authors.forename: E
authors.initials: E
authors.lastname: Małecka-Panas
authors.forename: I
authors.initials: I
authors.lastname: Płaneta-Małecka
authors.forename: W
authors.initials: W
authors.lastname: Rudnicka
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D001782:Blood Donors; D015153:Blotting, Western; D004415:Dyspepsia / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005756:Gastritis / Q000276:immunology / Q000382:microbiology; D005759:Gastroenteritis / Q000276:immunology; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008875:Middle Aged; D010437:Peptic Ulcer / Q000276:immunology / Q000382:microbiology; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins; C080866:cagA protein, Helicobacter pylori