New assay for glycated lipoproteins by high-performance liquid chromatography.
We developed an automated analytic system for glycated lipoprotein using high-performance liquid chromatography (HPLC) with an affinity boronate column and a gel permeation column. This system can measure glycated lipoprotein (glycated LDL and glycated HDL) in a small (5 microliters) sample of serum in a short time (40 min/sample). Recovery with this system was 92.1%. Therefore a large number of samples can be measured in clinical use. The system should contribute to an elucidation of the role of glycated lipoprotein in atherosclerosis.
Tanaka
A
A
Third Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Yui
K
K
Tomie
N
N
Baba
T
T
Tamura
M
M
Makita
T
T
Numano
F
F
Nakatani
S
S
Kato
Y
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood*
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL