Long-term survival effects of GDNF on neonatal rat facial motoneurons after axotomy.
Glial cell-line derived neurotrophic factor (GDNF) has survival promoting effects on axotomized neonatal motoneurons. We examined how long it could sustain motoneurons after postnatal day O (PND) facial nerve axotomy. GDNF, or cytochrome c as a negative control, were locally administered by Gelfoam implants at the time of axotomy and some were re-implanted on PND 14. The surviving motoneurons were quantified on PND 14 and 28. GDNF completely rescued lesioned motoneurons from axotomy-induced cell death at 14 days and was still effective (about 40%) at PND 28. GDNF also prevented axotomy-induced atrophy at both PND 14 and 28, indicating that the neurotrophic effects of GDNF on neonatal motoneurons are long-term.
Matheson
C R
CR
Department of Neurobiology, Amgen Inc., Thousand Oaks, CA 91320, USA.
Wang
J
J
Collins
F D
FD
Yan
Q
Q
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001369:Axons / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor; D008297:Male; D009046:Motor Neurons / Q000187:drug effects*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins