Thalamic-projecting preprocholecystokinin messenger RNA-expressing neurons in the dorsal column nuclei of the rat.
This study aimed at investigating the expression of preprocholecystokinin messenger RNA among thalamic-projecting neurons in the dorsal column nuclei of the rat. Thalamic-projecting neurons were identified by injection of cholera toxin subunit b into the ventroposterolateral nucleus. Following immunohistochemical detection of retrogradely transported tracer substance, the expression of preprocholecystokinin messenger RNA in the projection neurons of the dorsal column nuclei was detected by in situ hybridization, using autoradiographic visualization of a 35S-labeled RNA probe complementary to preprocholecystokinin messenger RNA. Many preprocholecystokinin-expressing neurons were seen in the dorsal column nuclei. A large proportion of these neurons were also labeled with cholera toxin. The double-labeled neurons, as well as neurons single-labeled with preprocholecystokinin messenger RNA or cholera toxin, were preferentially found within the middle region of the dorsal column nuclei, located just caudal to the obex. These findings demonstrate that neurons in the dorsal column nuclei express preprocholecystokinin messenger RNA, and show that these neurons provide a peptidergic projection from the dorsal column nuclei to the ventroposterolateral nucleus of the thalamus. These observations suggest that cholecystokinin may be involved in the transmission of somatosensory (tactile) information from the dorsal column nuclei to the thalamus.
Farnebo
S
S
Department of Cell Biology, Faculty of Health Sciences, University of Linköping, Sweden.
Hermanson
O
O
Blomqvist
A
A
D000818:Animals; D002766:Cholecystokinin / Q000235:genetics*; D002772:Cholera Toxin; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism / Q000502:physiology*; D009435:Synaptic Transmission; D013788:Thalamus / Q000502:physiology*
D011498:Protein Precursors; D012333:RNA, Messenger; C042082:preprocholecystokinin; D002766:Cholecystokinin; D002772:Cholera Toxin