title: Caveolin and MAL, two protein components of internal detergent-insoluble membranes, are in distinct lipid microenvironments in MDCK cells.
abstract: The MAL proteolipid and caveolin have been identified as components of internal detergent-insoluble membrane microdomains enriched in glycolipids and cholesterol. We have addressed the study of the glycolipid-enriched membranes in cells expressing endogenously only either MAL (Jurkat T cells) or caveolin (epithelial A498 cells) and in polarized MDCK cells which express both proteins simultaneously. Subcellular fractionation by centrifugation to equilibrium in sucrose gradients of Triton X-100 cell extracts from Jurkat and A498 cells revealed that MAL and caveolin are incorporated in detergent-insoluble buoyant membranes independently of the expression of each other and indicated the existence in these cells of insoluble membrane microdomains with either MAL or caveolin. Immunofluorescence analysis in MDCK cells indicated that both MAL and caveolin were located in the Golgi region, whereas caveolin was found in addition at the cell surface. Biochemical analysis in these cells revealed the existence of distinct membrane microenvironments differentially susceptible to detergent solubilization containing either internal MAL or internal plus surface caveolin. The observed heterogeneity within the internal glycolipid-enriched membrane fraction suggests the existence of distinct specialized lipid microenvironments in MDCK cells.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Consejo Superior de Investigaciones Científicas, Cantoblanco, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Millán
authors.forename: R
authors.initials: R
authors.lastname: Puertollano
authors.forename: L
authors.initials: L
authors.lastname: Fan
authors.forename: M A
authors.initials: MA
authors.lastname: Alonso
mesh_terms: D000818:Animals; D051242:Caveolin 1; D022461:Caveolins; D002451:Cell Compartmentation; D002460:Cell Line; D016764:Cell Polarity; D003902:Detergents; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins; D009185:Myelin Proteins; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D017830:Octoxynol; D011510:Proteolipids / Q000378:metabolism*; D012995:Solubility
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D003902:Detergents; C569026:MAL protein, human; D008563:Membrane Lipids; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009185:Myelin Proteins; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D011510:Proteolipids; D017830:Octoxynol