Effects of epidermal growth factor and insulin on the activity of N-acetylglucosaminyltransferase V.
When quiescent rat hepatocellular carcinoma 7919 cells were treated with epidermal growth factor (EGF) or insulin (stimulators of receptor tyrosine kinase activity), the activity of N-acetylglucosaminyltransferase V was increased. The effect of EGF reached a maximum after 10 min and remained high for 30 min, while the effect of insulin reached a maximum after 5 min and decreased after 15 min. Preincubation of the cells with 1-O-octadecyl-2-O-methylglycerophosphocholine (Et18-OH3), which blocked the activation of mitogen-activated protein kinase by EGF, also blocked the activation of N-acetylglucosamyltransferase V by this hormone, whereas the activation of N-acetylglucosamyltransferase V by insulin could not be blocked by Et18-OH3. Our results suggest that N-acetylglucosamyltransferase V may be regulated by different receptor protein tyrosine kinase pathways.
Wang
Q
Q
Gene Research Centre, Key Laboratory of Glycoconjugate, Ministry of Public Health, and Department of Biochemistry, Shanghai Medical University, Shanghai, People's Republic of China 200032.
Zhou
D
D
Shao
D
D
Shen
Z
Z
Gu
J
J
D000818:Animals; D002240:Carbohydrate Sequence; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D010742:Phospholipid Ethers / Q000494:pharmacology; D051381:Rats
D004791:Enzyme Inhibitors; D007328:Insulin; D009363:Neoplasm Proteins; D010742:Phospholipid Ethers; C026659:edelfosine; D004815:Epidermal Growth Factor; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase