title: [Splenic lymphoma with villous lymphocytes: morphologic, immunologic and molecular study. Report of three cases].
abstract: Splenic lymphoma with villous lymphocytes (SLVL) is a low grade lymphoproliferation characterized by a massive splenomegaly, an absence of lymphadenopathy and the presence in the peripheral blood of atypical B-lymphocytes with hairy-cell appearance. We have studied the morphological, immunological and molecular characteristics of 3 cases of SLVL. SLVL presented on blood smears characteristic irregularities of the plasma membrane consisting in thin and short villi unevenly distributed. The main phenotype was CD5-, CD11c+, and CD25-, but individual SLVL cases can not be identified by using immunohistochemical criteria alone. Clonal rearrangements of the immunoglobulin heavy chain gene were found in all 3 cases and in one case presented a bcl2-JH rearrangement. SLVL are clonal B-cell lymphoproliferations and can be associated with t(14; 18) translocation.
authors.affiliation: Laboratoire d'Anatomie Pathologique, Hôpital Haut-Levêque, CHU Bordeaux.
authors.forename: E
authors.initials: E
authors.lastname: Labouyrie
authors.forename: A G
authors.initials: AG
authors.lastname: Chartois-Leauté
authors.forename: P
authors.initials: P
authors.lastname: Dubus
authors.forename: E
authors.initials: E
authors.lastname: Duchayne
authors.forename: P
authors.initials: P
authors.lastname: Brousset
authors.forename: J
authors.initials: J
authors.lastname: Ceccaldi
authors.forename: M
authors.initials: M
authors.lastname: Capbern
authors.forename: P
authors.initials: P
authors.lastname: Fialon
authors.forename: P
authors.initials: P
authors.lastname: Soubeyran
authors.forename: G
authors.initials: G
authors.lastname: Delsol
authors.forename: A
authors.initials: A
authors.lastname: de Mascarel
authors.forename: J P
authors.initials: JP
authors.lastname: Merlio
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D019254:Genes, bcl-2; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008297:Male; D013160:Splenic Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D013163:Splenomegaly / Q000276:immunology / Q000473:pathology
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm