title: Protection against tetanus toxin in mice nasally immunized with recombinant Lactococcus lactis expressing tetanus toxin fragment C.
abstract: Mice inoculated intranasally (i.n.) with a recombinant strain of live Lactococcus lactis expressing tetanus toxin fragment C (TTFC), produced both serum and secretory antibodies to TTFC. Killed bacteria which had accumulated TTFC intracellularly in vitro also elicited protective serum antibody responses. There was no requirement for either colonization or invasion of the mucosa. In addition secretory antibody responses in the lung and nasal tissues were elicited after i.n. inoculation in the presence of an adjuvant.
authors.affiliation: Department of Pathology, University of Cambridge, UK.
authors.forename: P M
authors.initials: PM
authors.lastname: Norton
authors.forename: J M
authors.initials: JM
authors.lastname: Wells
authors.forename: H W
authors.initials: HW
authors.lastname: Brown
authors.forename: A M
authors.initials: AM
authors.lastname: Macpherson
authors.forename: R W
authors.initials: RW
authors.lastname: Le Page
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D005822:Genetic Vectors; D018928:Immunity, Mucosal; D013294:Lactococcus lactis; D051379:Mice; D008810:Mice, Inbred C57BL; D013743:Tetanus Antitoxin / Q000097:blood*; D013744:Tetanus Toxin / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D013743:Tetanus Antitoxin; D013744:Tetanus Toxin; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic