title: Evaluation of a rapid, quantitative cardiac troponin I immunoassay.
abstract: We evaluated a rapid, quantitative immunoassay for the detection of cardiac troponin I. Coefficient of variation is between 1.29 and 13.63% for intra-assay and between 3.88 and 10.15% for inter-assay imprecision. Linearity is given up to 35 micrograms/l. Possible interfering substances (haemoglobin, bilirubin, triacylglycerol and rheuma factors) do not disturb the assay. The analyte is stable under normal storage conditions (+20 degrees C/48 h and +4 degrees C/l week) with decrease up to 30% after 3 months at -20 degrees C. Reference value for apparently healthy individuals is < 0.1 microgram/l. In plasma cardiac troponin I is measured up to 30% depressed compared to serum. Comparison with another cardiac troponin I assay (y = 0.92x + 2.42, r = 0.940) and cardiac troponin T is good with y = 6.61x - 1.94, r = 0.91 for the first generation cardiac troponin T assay and y = 5.59x - 0.68, r = 0.87 for the second generation cardiac troponin T assay. In summary, the evaluated assay is fast, easy to perform, and can be used not only in a specialized laboratory, but is also suitable for emergency laboratory or smaller laboratory units.
authors.affiliation: Institut für Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar der Technischen Universität München, Germany.
authors.forename: L P
authors.initials: LP
authors.lastname: Kuhr
authors.forename: H
authors.initials: H
authors.lastname: Baum
authors.forename: R
authors.initials: R
authors.lastname: Schweigert
authors.forename: G
authors.initials: G
authors.lastname: Hafner
authors.forename: W
authors.initials: W
authors.lastname: Prellwitz
authors.forename: D
authors.initials: D
authors.lastname: Neumeier
mesh_terms: D004355:Drug Stability; D015200:Fluoroimmunoassay / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D016014:Linear Models; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis; D009206:Myocardium / Q000737:chemistry*; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019210:Troponin I / Q000097:blood*
chemical_list: D019210:Troponin I