Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II.
A human macrophage line, designated K1m, has been established from peripheral blood. K1m expresses a number of lineage-specific markers as well as a broad array of intercellular adhesion molecules. In particular, K1m expresses high levels of human leucocyte antigen (HLA) class I and class II. In response to ligation of HLA class II (HLA-DR), but not in response to ligation of HLA class 1, K1m forms tighter homotypic aggregates and develops a striking 'stellate' culture phenotype. K1m also expresses Fc receptors for immunoglobulin G (IgG) (CD64, CD32, and CD16) and can be shown to phagocytose polystyrene latex beads, as well as neuroblastoma cells in the presence of tumour-specific monoclonal antibody (mAb). The K1m cell line should therefore prove useful for studying both signalling through macrophage HLA class II and immunophagocytosis.
Dialynas
D P
DP
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA.
Lee
M J
MJ
Shao
L E
LE
Tan
P C
PC
Yu
J
J
D000920:Antibody-Dependent Cell Cytotoxicity; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line / Q000276:immunology; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology
D000954:Antigens, Surface; D006684:HLA-DR Antigens