Serotype of Nigerian rotavirus strains.
Three hundred and fourteen stool samples collected from children < 5 years between December 1993 and August 1995 were analysed by PAGE, ELISA, PCR and Dot-blot hybridization technique for electropherotype and serotype distribution of rotavirus infection among Nigerian paediatric patients. 14.3% of the children were positive for rotavirus antigen. Children aged 6-9 months were most often infected, accounting for 35.6% of all positive samples, 91.1% of rotavirus-positive samples could be serotyped. Serotypes G2, G4 and G8 were not detected. Serotype G3 predominated (62.5%) in southern Nigeria, while mixed infection specificity was more widespread (63.6%) in northern Nigeria. The presence of some untypeable samples may indicate serotypes which the serotype-specific primers and cDNA probes used could not detect. Electropherotypes of 26 (57.7%) of the positive samples were determined. Two and 3 migration patterns were observed among the short and long-pattern electropherotypes, respectively. Implications for vaccine development and utilization in the country are discussed.
Adah
M I
MI
Institute of Medical Microbiology and Virology, Ruhr-Universität, Bochum, Germany.
Rohwedder
A
A
Olaleye
O D
OD
Durojaiye
O A
OA
Werchau
H
H
D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D017720:Molecular Epidemiology; D009549:Nigeria / Q000453:epidemiology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000235:genetics / Q000276:immunology; D016036:Seroepidemiologic Studies; D012703:Serotyping
D012367:RNA, Viral