Analysis of the DPC4 gene in gastric carcinoma.
Allelic loss on chromosome 18q is involved in the progression of gastric carcinoma. Recently, DPC4 (deleted in pancreatic carcinoma, locus 4), a candidate tumor suppressor gene, has been localized at 18q21.1. This gene is inactivated in nearly one half of pancreatic carcinomas. We tested for DPC4 gene mutations and allelic status at 18q21 in 30 primary gastric carcinomas and 5 gastric carcinoma cell lines. Polymerase chain reaction single-strand conformation polymorphism and sequencing analyses revealed no DPC4 mutations in any of the primary tumors or cell lines. Homozygous deletion of DPC4 was observed in only 1 (MKN-45) of the 5 (20%) cell lines. This suggests that the target gene for loss on 18q is not DPC4. The true tumor suppressor gene, encoded near DPC4, has yet to be identified.
Nishizuka
S
S
Department of Pathology, Iwate Medical University School of Medicine, Morioka.
Tamura
G
G
Maesawa
C
C
Sakata
K
K
Suzuki
Y
Y
Iwaya
T
T
Terashima
M
M
Saito
K
K
Satodate
R
R
D002872:Chromosome Deletion; D002887:Chromosomes, Human, Pair 18; D004268:DNA-Binding Proteins; D016147:Genes, Tumor Suppressor; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D051901:Smad4 Protein; D013274:Stomach Neoplasms / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*
D004268:DNA-Binding Proteins; C497796:SMAD4 protein, human; D051901:Smad4 Protein; D015534:Trans-Activators