title: Role of the cytoskeleton in endocytosis of the yeast maltose transporter.
abstract: Certain components of the cytoskeleton play a role in yeast fluid-phase endocytosis as well as in endocytosis of the alpha-factor when this pheromone is bound to its 7-transmembrane segment receptor. The yeast maltose transporter is a 12-transmembrane segment protein that, under certain physiological conditions, is degraded in the vacuole after internalization by endocytosis. In this work, the possible role of the cytoskeleton in endocytosis of this transporter has been investigated. Using mutants defective in beta-tubulin, actin and the actin-binding proteins Sac6 and Abp85. as well as nocodazole, which inhibits formation of microtubules, we have shown that actin microfilaments are involved in endocytosis of the maltose transporter whereas microtubules are not.
authors.affiliation: Instituto de Investigaciones Biomédicas, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Peñalver
authors.forename: L
authors.initials: L
authors.lastname: Ojeda
authors.forename: E
authors.initials: E
authors.lastname: Moreno
authors.forename: R
authors.initials: R
authors.lastname: Lagunas
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D004705:Endocytosis; D005656:Fungal Proteins / Q000378:metabolism*; D017353:Gene Deletion; D008320:Maltose / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D002352:Carrier Proteins; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; D014404:Tubulin; C067575:maltose transport system, S cerevisiae; C026742:plastin; D008320:Maltose