How to overcome the disturbing effects of human anti-mouse antibodies (HAMA) on in vitro assays.
The development of human anti-mouse antibodies (HAMA) is a common immune response in patients with ovarian carcinoma after repeated injections of murine anti-CA 125 monoclonal antibodies for immunoscintigraphy. As a tumor marker with significant diagnostic value CA 125 is routinely measured in the follow-up of tumor patients by immunoradiometric assays (IRMA) based on murine anti-CA 125 monoclonal antibodies. HAMA may cause false-positive findings in a CA 125-IRMA. In this report our group demonstrates a simple way of eliminating HAMA by protein A/G affinity chromatography allowing the reliable detection of the tumor marker CA 125 in the serum of patients with ovarian carcinoma.
Oltrogge
J B
JB
Department of Biological Chemistry, University Medical Center, Frankfurt/Main, Germany.
Baum
R P
RP
Lema
K N
KN
Donnerstag
B
B
Hör
G
G
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000097:blood / Q000737:chemistry / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D000912:Antibodies, Neoplasm / Q000097:blood / Q000737:chemistry / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology* / Q000627:therapeutic use; D018394:CA-125 Antigen / Q000032:analysis* / Q000276:immunology* / Q000627:therapeutic use; D002846:Chromatography, Affinity; D005189:False Positive Reactions; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods; D051379:Mice; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology* / Q000628:therapy
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D018394:CA-125 Antigen