Effects of UV-B radiation on a hereditary suture cataract in mice.
UV-B (290-320 um, lambda max = 305 nm) radiation and the Cat2ns (suture cataract) mutation in mice affect both the anterior lens epithelium and the formation of the suture. A low dose of UV-B radiation (2.2 Jcm-2) induces similar anterior subcapsular and cortical lens opacities in wild type as in heterozygous mutant mice. The UV-B treatment of the mutant lenses, however, leads to an increase in the number of epithelial cell layers in the anterior central part as compared to the wild type indicating a more severe form of the cataract formation in mutants. In addition, mutants demonstrate a predisposition for a rupture of the posterior lens capsule, because from 2.9 Jcm-2 and higher, this phenomenon could always be observed in the UV-B treated mutants, but never in the treated wild type mice. The protein biochemical analyses were performed by gel electrophoresis and isoelectric focusing of extracts of total lenses or from defined areas of the lens (lens slice technique). These covered the patterns of those proteins already synthesized before irradiation, which in irradiated lenses in no case evidenced a difference to the untreated control, neither in the wild type nor in the mutants. In contrast, by analysing specifically those proteins, which are synthesised after irradiation, in both treated groups a protein with a molecular mass of about 31 kDa becomes discernable in both treated groups. In addition, the cataractous lenses demonstrate a significantly enhanced overall synthesis of water-soluble proteins after irradiation, which might promote the rupture of the posterior capsule at the posterior pole. The present study offers for the first time the possibility to discriminate between endogeneous (genetic) effects and exogeneous (environmental) effects in cataractogenesis and to study their interactive effects. The first set of experiments demonstrated a clear intensification of the hereditary cataract by the UV-B treatment. The study supports the hypothesis that environmental stress (like UV-B radiation) enhanced the severity of genetically triggered eye disease.
Forker
C
C
Department of Experimental Ophthalmology, University of Bonn, Germany.
Wegener
A
A
Graw
J
J
D000818:Animals; D002386:Cataract / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D003459:Crystallins / Q000378:metabolism / Q000528:radiation effects; D004848:Epithelium / Q000473:pathology; D005260:Female; D007903:Lens Capsule, Crystalline / Q000473:pathology / Q000528:radiation effects; D007904:Lens Cortex, Crystalline / Q000473:pathology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D014466:Ultraviolet Rays / Q000009:adverse effects*
D003459:Crystallins