Confocal laser scanning microscopy of porcine skin: implications for human wound healing studies.
The structure of porcine skin as examined by light microscopy is reviewed and its similarities to and differences from human skin are highlighted. Special imaging techniques and staining procedures are described and their use in gathering morphological information in porcine skin is discussed. Confocal laser scanning microscopy (CLSM) was employed to examine the structure of porcine skin and the findings are presented as an adjunct to the information already available in the literature. It is concluded that CLSM provides valuable additional morphological information to material examined by conventional microscopy and is useful for wound healing studies in the porcine model.
Vardaxis
N J
NJ
Department of Medical Laboratory Sciences, Royal Melbourne Institute of Technology, Bundoora, Victoria, Australia.
Brans
T A
TA
Boon
M E
ME
Kreis
R W
RW
Marres
L M
LM
D000818:Animals; D003094:Collagen / Q000648:ultrastructure; D004396:Coloring Agents; D004195:Disease Models, Animal; D004549:Elastin / Q000648:ultrastructure; D004817:Epidermis / Q000648:ultrastructure; D005260:Female; D008264:Macrophages / Q000166:cytology; D008407:Mast Cells / Q000166:cytology; D018613:Microscopy, Confocal; D009130:Muscle, Smooth / Q000166:cytology; D012867:Skin / Q000166:cytology* / Q000648:ultrastructure; D013552:Swine / Q000033:anatomy & histology*; D014945:Wound Healing
D004396:Coloring Agents; D003094:Collagen; D004549:Elastin