CDRH3 length is the target of selection of disease-associated IgM autoantibodies.
The experiments outlined here provide evidence for positive selection of B-1 lymphocytes secreting IgM autoantibodies in the autoimmune mev mice as a result of the reduced threshold of responsiveness to autoantigens because of PTP1C deficiency, which is targeted at the CDR3 length of the variable region of the heavy chain. In addition, characteristic differences in the size, hydrophobicity pattern, and relative local charge of the CDR3 of the heavy chain of disease-associated IgM autoantibodies from moth-eaten mice help distinguish them from physiological natural autoantibodies present in normal mice.
Lipsanen
V
V
Department of Pathobiology, University of Guelph, Ontario, Canada.
Walter
B
B
Emara
M
M
Siminovitch
K
K
Lam
J
J
Kaushik
A
A
D000818:Animals; D001323:Autoantibodies / Q000235:genetics* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data
D001323:Autoantibodies; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region