Cloning and sequence analysis of putative histidine protein kinases isolated from Lactococcus lactis MG1363.
Eight recombinant plasmids harboring chromosomal fragments of Lactococcus lactis MG1363 were shown to phenotypically suppress a histidine protein kinase (HPK) deficiency in either of two different E. coli strains. Sequence analysis of the plasmid inserts revealed five different complete or partial open reading frames (ORFs) specifying proteins with high similarity to HPKs. One of the plasmids also harbored an additional ORF, unrelated to HPKs, with suppressing activity.
O'Connell-Motherway
M
M
Department of Microbiology, University College, Cork, Ireland.
Fitzgerald
G F
GF
van Sinderen
D
D
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D000071677:Histidine Kinase; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics*; D011995:Recombination, Genetic; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013489:Suppression, Genetic
D004269:DNA, Bacterial; D011494:Protein Kinases; D000071677:Histidine Kinase