title: Detection of post-translational sulfation of alpha-5 beta-1 integrin and its role in integrin-fibronectin binding.
abstract: Fibronectins are glycoproteins of the extracellular matrix composed of two 220-kDa polypeptide chains named A and B bound by two disulfide bridges. Both chains when digested with proteolytic enzymes give rise to six different domains named I to VI that are involved in the ligand properties of this molecule. Fibronectins bind fibrin, collagen, glycosaminoglycan residues and several integrins. In this study, using metabolic radiolabeling of alpha 5 beta 1 integrin with sodium sulfate, an immunoprecipitation reaction, inhibition of sulfate incorporation and a fibronectin-binding assay, we were able to detect this integrin as a sulfated molecule and this sulfation appears to regulate the integrin-fibronectin binding.
authors.affiliation: Instituto Ludwig de Pesquisas sobre o Cancer, São Paulo, Brasil.
authors.forename: S S
authors.initials: SS
authors.lastname: Veiga
authors.forename: M C
authors.initials: MC
authors.lastname: Elias
authors.forename: W
authors.initials: W
authors.lastname: Gremski
authors.forename: M A
authors.initials: MA
authors.lastname: Porcionatto
authors.forename: H B
authors.initials: HB
authors.lastname: Nader
authors.forename: R R
authors.initials: RR
authors.lastname: Brentani
mesh_terms: D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism; D005353:Fibronectins / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D017469:Receptors, Fibronectin / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism
chemical_list: D005353:Fibronectins; D017469:Receptors, Fibronectin; D013431:Sulfates; D005337:Fibrin; D003094:Collagen