Duplex PCR of the Y-27H39 and HPRT loci with reference to Japanese population data on the HPRT locus.
Using a duplex polymerase chain reaction (PCR) system, we successfully amplified GATA microsatellite loci Y-27H39 and HPRT on the Y and X chromosomes, respectively, and detected their alleles with a digoxigenin-labeled (GATA)6 probe. Among 450 unrelated Japanese including 225 females, we found 6 HPRT alleles ranging from 155 to 175 nucleotides with a simple repeat structure comprising 11 to 16 repeats. The duplex PCR is highly informative in Japanese.
Kishida
T
T
Department of Forensic Medicine, Oita Medical University, Japan.
Wang
W
W
Fukuda
M
M
Tamaki
Y
Y
D000483:Alleles; D044466:Asian People; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007564:Japan; D008297:Male; D018895:Microsatellite Repeats; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012732:Sex Determination Analysis; D014960:X Chromosome; D014998:Y Chromosome
D007041:Hypoxanthine Phosphoribosyltransferase