title: VP5 of infectious bursal disease virus is not essential for viral replication in cell culture.
abstract: Infectious bursal disease virus (IBDV), a member of the Birnaviridae family, encodes in its bisegmented double-stranded RNA genome four structural virion proteins, VP1, VP2, VP3, and VP4, as well as a nonstructural protein, VP5. Recently, the establishment of an infectious cRNA system for IBDV has been described (E. Mundt and V. N. Vakharia, Proc. Natl. Acad. Sci. USA 93:11131-11136, 1996). Here, we report the isolation of a VP5- IBDV mutant constructed by site-directed mutagenesis of the methionine start codon of VP5, followed by cRNA transfection. The resulting virus mutant was replication competent in cell culture, which indicates that VP5 is not required for productive replication of IBDV. Absence of VP5 expression was verified by lack of reactivity with newly established anti-VP5 monoclonal antibodies and polyclonal sera. VP5- IBDV exhibited a delay in replication in chicken embryo cells compared to the VP5+ parental virus. However, final yields were similar. Our results thus show that VP5 is nonessential for IBDV replication, which makes it a prime candidate for the construction of deleted, marked vaccines.
authors.affiliation: Institutes of Molecular and Cellular Virology, Friedrich Loeffler Institutes, Federal Research Center for Virus Diseases of Animals, Insel Riems, Germany. mundt@rie.bfav.de
authors.forename: E
authors.initials: E
authors.lastname: Mundt
authors.forename: B
authors.initials: B
authors.lastname: Köllner
authors.forename: D
authors.initials: D
authors.lastname: Kretzschmar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004279:DNA, Viral; D017353:Gene Deletion; D007243:Infectious bursal disease virus / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011817:Rabbits; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D004279:DNA, Viral; D017361:Viral Nonstructural Proteins