A comparison of monoclonal antibody immobilization of platelet antigen (MAIPA) and immunobead methods for detection of GPIIb/IIIa antiplatelet antibodies in immune thrombocytopenic purpura.
In this study we have compared two assays for the detection of autoantibodies GpIIb/IIIa, platelet bound and in serum, in immune thrombocytopenic purpura (ITP). Both assays were found to have a similar sensitivity, but the monoclonal antibody immobilization of platelet antigen (MAIPA) assay was more reproducible than the immunobead assay. The MAIPA and immunobead assay demonstrated an 81% concordance of results for serum antibody detection and a 78% concordance for platelet-associated antibody detection, with an 8-12% incidence of false positive or negative results.
Crossley
A
A
Department of Haematology, Medical School, University of Newcastle upon Tyne, UK.
Calvert
J E
JE
Taylor
P R
PR
Dickinson
A M
AM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D016824:Antigens, Human Platelet / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D006801:Humans; D007163:Immunosorbent Techniques; D008875:Middle Aged; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000175:diagnosis* / Q000276:immunology; D017186:Titrimetry
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D001323:Autoantibodies; D019039:Platelet Glycoprotein GPIIb-IIIa Complex