title: Presenilin 1 interaction in the brain with a novel member of the Armadillo family.
abstract: One approach to understanding the function of presenilin 1 (PS1), is to discover those proteins with which it interacts. Evidence for a function in developmental patterning came from C. elegans, in which a PS homologue was identified by screening for suppressors of a mutation in Notch/lin-12, a gene which specifies cell fate. However, this genetic experiment cannot determine which proteins directly interact with PS1. Therefore, we utilized the two hybrid system and confirmatory co-immunoprecipitations to identify a novel catenin, termed gamma-catenin, which interacts with PS1 and is principally expressed in brain. The catenins are a gene family related to the Armadillo gene in Drosophila, some of which appear to have dual roles-they are components of cell-cell adherens junctions, and may serve as intermediates in the Wingless (Wg) signaling pathway, which, like Notch/lin-12, is also responsible for a variety of inductive signaling events. In the non-neuronal 293 cell line, PS1 interacted with gamma-catenin, the family member with the greatest homology to Armadillo. Wg and Notch interactions are mediated by the Disheveled gene, which may form a signaling complex with PS1 and Wg pathway intermediates to regulate the function of the Notch/lin-12 gene.
authors.affiliation: Department of Neurology, Brigham and Women's Hospital, Boston, MA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.forename: U
authors.initials: U
authors.lastname: Liyanage
authors.forename: M
authors.initials: M
authors.lastname: Medina
authors.forename: C
authors.initials: C
authors.lastname: Ho
authors.forename: A D
authors.initials: AD
authors.lastname: Simmons
authors.forename: M
authors.initials: M
authors.lastname: Lovett
authors.forename: K S
authors.initials: KS
authors.lastname: Kosik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D051180:Desmoplakins; D004330:Drosophila / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017344:Genes, Insect; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D053764:Presenilin-1; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators; D051176:beta Catenin; D051185:gamma Catenin
chemical_list: D003598:Cytoskeletal Proteins; D051180:Desmoplakins; D008565:Membrane Proteins; D053764:Presenilin-1; D015534:Trans-Activators; D051176:beta Catenin; D051185:gamma Catenin