The binding ability to matrix proteins and the inhibitory effects on cell adhesion of synthetic peptides derived from a conserved sequence of integrins.
The beta peptide (113-125), derived from a conserved sequence of the beta subunit of integrins, was synthesized to investigate its adhesive properties to matrix proteins and the effects on cell adhesion to immobilized fibronectin. In this study, we observed that the biotinylated beta peptide was able to bind efficiently to immobilized fibronectin, fibrinogen, collagen Type I and vitronectin with different degrees of affinity. It was also demonstrated that biotinylated fibronectin or fibrinogen could bind to the coated beta peptide. This kind of binding, which might be non-covalent linkage, was partially blocked by coincubation with the peptide GRGDS or EDTA, but not by SDGRG. Cell adhesion experiments were performed to study the effect of the beta peptide. The data showed that the beta peptide partially inhibited both fibroblast L929 and MC3T3-E1 osteoblastic cells from adhering to immobilized fibronectin in a dosage-dependent manner. In the presence of 100 microM concentration of the beta peptide, the inhibition rate of cell adhesion was 34% for fibroblast L929 cells and 54.1% for MC 3T3-E1 osteoblastic cells. This research suggests that the beta peptide might act independently as an adhesive region of the beta subunit of integrins and may occupy the cell-binding site within fibronectin.
Liu
Y K
YK
Bionic Design Group, National Institute for Advanced Interdisciplinary Research (NAIR), Tsukuba Research Center, Ibaraki.
Nemoto
A
A
Feng
Y
Y
Uemura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D017124:Conserved Sequence; D004492:Edetic Acid / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D016023:Integrins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology*; D011485:Protein Binding
D000970:Antineoplastic Agents; D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D016023:Integrins; D009842:Oligopeptides; D010455:Peptides; C047981:arginyl-glycyl-aspartic acid; D005340:Fibrinogen; C049554:glycyl-arginyl-glycyl-aspartyl-serine; D004492:Edetic Acid; D002118:Calcium