Ascorbate stabilization is stimulated in rho(0)HL-60 cells by CoQ10 increase at the plasma membrane.
Long-term treatment with ethidium bromide of HL-60 cells induced a mitochondria-deficient rho degree cell line, where mitochondrial DNA can not be identified by PCR and cytochrome c oxidase activity was 80% decreased. These cells showed a progressive increase of ascorbate stabilization which was 52% higher in the established rho degree HL-60 cells. Both CoQ10 and NADH-ascorbate free radical reductase of the plasma membrane were increased in rho(0)HL-60 cells compared to parental cells, while NADH-cytochrome c reductase was unchanged. CoQ10 is a component of the ascorbate stabilization activity in the plasma membrane that would provide both a mechanism to deplete the excess of NADH produced in rho(0)HL-60 cells and for resistance to oxidative stress.
Gómez-Díaz
C
C
Departamento de Biología Celular, Universidad de Córdoba, Spain.
Villalba
J M
JM
Pérez-Vicente
R
R
Crane
F L
FL
Navas
P
P
D001205:Ascorbic Acid / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism*; D003067:Coenzymes; D004261:DNA Replication / Q000187:drug effects; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004996:Ethidium / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007364:Intercalating Agents / Q000494:pharmacology; D008861:Microsomes / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology
D003067:Coenzymes; D004272:DNA, Mitochondrial; D007364:Intercalating Agents; D009243:NAD; D014451:Ubiquinone; D009247:NADH, NADPH Oxidoreductases; C023927:monodehydroascorbate reductase (NADH); D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; C024989:coenzyme Q10; D004996:Ethidium; D001205:Ascorbic Acid