Regional distribution of phospholipids and polyphosphatidyl inositides in the rabbit's spinal cord.
The plasticity of the membrane phospholipids in general and stimulated phosphoinositides turnover in particular are the subjects in a variety of neural paradigms studying the molecular mechanisms of neuronal changes under normal and pathological conditions. The regional modifiability of phospholipids (SM, PC, PS, PI, PA + DG, PE), polyphosphatidylinositides (PI, PIP, PIP2) and diacylglycerol-dependent incorporation of CDP-choline into phosphatidylcholine in the gray matter, white matter, dorsal horns, intermediate zone and ventral horns of the rabbit's spinal cord was studied. We have found 1. a significant increase in the concentration of SM, PC, PS, DG + PA and PE in the white matter in comparison to the gray one, 2. the highest concentration of the outer membrane leaflet-bound phospholipids in the dorsal horns and the inner membrane phospholipids in the intermediate zone in comparison to the gray matter, 3. a substantial amount of labeled polyphosphatidylinositides (poly-PI(s)) in the spinal cord white matter with descending order PIP > PI > PIP2, 4. similar incorporation of myo-2-[3H]inositol into all poly-PI(s) in ventral horns and intermediate zone, but a different, lower incorporation into PI and PIP and higher into PIP2 in the dorsal horns, 5. higher diacylglycerol-dependent incorporation of CDP-choline into PC in the regionally undivided gray matter than in the white matter taken as a whole, 6. the high proportion of diacylglycerol-dependent incorporation of CDP-choline into PC in both the ventral and dorsal horns, whereas that in the intermediate zone remained low.
Lukácová
N
N
Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovak Republic.
Marsala
J
J
D000818:Animals; D008161:Lumbosacral Region; D008297:Male; D010716:Phosphatidylinositols / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D011817:Rabbits; D015290:Second Messenger Systems / Q000502:physiology; D013116:Spinal Cord / Q000737:chemistry*; D009435:Synaptic Transmission / Q000502:physiology
D010716:Phosphatidylinositols; D010743:Phospholipids