Insertion of MAP4-VP1 peptide into lipid monolayers and bilayers.
Myscibility of dipalmitoylphosphatidylcholine (DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipine (CL) and dipalmitoylphosphatidylethanolamine (DPPE) was studied using monomolacular layers of different compositions. The influence of MAP4-VP1 peptide construct related to HAV protein in mixed monolayers of the above cited lipid components was determined following penetration kinetics and compression isotherms. Moreover, liposomes with the same composition as monolayers were saturated with ANS or DPH and incubated with MAP4VP1, and polarization values as well as transition temperatures were determined. In general interaction is maximum with DPPC/DPPG mono and bilayers, followed by DPPC/CL. In these systems electrostatic repulsive forces seem to play a strong role.
Mestres
C
C
Physico-chemical Unit, Facultat Farmàcia, Barcelona, Spain.
Haro
I
I
Reig
F
F
Alsina
M A
MA
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002308:Cardiolipins / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D010712:Phosphatidic Acids / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D015678:Viral Structural Proteins / Q000737:chemistry*
D002308:Cardiolipins; D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D010712:Phosphatidic Acids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; C096539:VP1 protein, hepatitis A virus; D015678:Viral Structural Proteins; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C030345:1,2-dipalmitoylphosphatidylglycerol