Purification of an expressed insect transferrin from cell culture media using high-capacity Ni(2+)-dipicolylamine gel.
Vertebrate transferrin is a well characterized iron transport protein. In contrast, little is known concerning the role of transferrin in insects. Yet, study of iron metabolism in insects could give insights into strategies for insect control, particularly for insects that transmit disease.
Winzerling
J J
JJ
Arizona Research Laboratories, Center for Insect Science and Biotechnology Program, University of Arizona, Tuscon 85721, USA.
Pham
D Q
DQ
Kunz
S
S
Law
J H
JH
Porath
J
J
D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods*; D003470:Culture Media; D005782:Gels / Q000737:chemistry; D019476:Insect Proteins / Q000235:genetics / Q000302:isolation & purification*; D018402:Manduca / Q000737:chemistry* / Q000235:genetics; D009942:Organometallic Compounds / Q000737:chemistry*; D010847:Picolines / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification; D018411:Spodoptera / Q000166:cytology; D014168:Transferrin / Q000235:genetics / Q000302:isolation & purification*
D003470:Culture Media; D005782:Gels; D019476:Insect Proteins; D009942:Organometallic Compounds; D010847:Picolines; D011993:Recombinant Fusion Proteins; D014168:Transferrin; C103101:nickel dipicolylamine