Expression and nocturnal increase of type II iodothyronine deiodinase mRNA in rat pineal gland.
It has been demonstrated that thyroxine deiodinating activity is present in rat pineal gland, and its activity increases significantly during the night time. We have studied whether mRNA for type II iodothyronine deiodinase is expressed in rat pineal gland and whether the nocturnal rise of pineal T4 deiodinating activity is due to the change in type II iodothyronine deiodinase mRNA level. Reverse transcription-polymerase chain reaction amplification and Northern blot analyses have demonstrated that type II iodothyronine deiodinase mRNA is expressed in rat pineal gland and its mRNA level increases markedly at midnight. These results suggest that the nocturnal rise in pineal T4 deiodinating activity is due to the change in type II iodothyronine deiodinase mRNA level.
Murakami
M
M
First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Hosoi
Y
Y
Negishi
T
T
Kamiya
Y
Y
Ogiwara
T
T
Mizuma
H
H
Yamada
M
M
Iriuchijima
T
T
Mori
M
M
D000818:Animals; D015152:Blotting, Northern; D007453:Iodide Peroxidase / Q000235:genetics*; D008297:Male; D010870:Pineal Gland / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
D012333:RNA, Messenger; D007453:Iodide Peroxidase