Estimation of aneuploidy levels for 8, 15, 18, X and Y chromosomes in 97 human sperm samples using fluorescence in situ hybridization.
OBJECTIVE
To estimate the mean frequency of aneuploidy levels of chromosomes 8, 15, 18, X, and Y in human sperm, while minimizing the effect of individual factors by analyzing sperm samples from a large set of patients.


METHODS
Prospective randomized analysis of sperm nuclei by fluorescence in situ hybridization.


METHODS
University-based laboratory for reproductive biology.


METHODS
One hundred two patients with a large distribution of sperm parameters, randomly selected from volunteers who had presented seeking a semen analysis.


METHODS
The sperm samples were prepared for fluorescence in situ hybridization.


METHODS
The disomy frequencies for chromosomes 8, 15, 18, and sex chromosomes were determined using fluorescence in situ hybridization.


RESULTS
The mean frequencies of disomy for autosomes were 0.18% for chromosome 8, 0.06% for chromosome 15, 0.2% for chromosome 18, and 0.24% for gonosomes (XX, 0.04%; YY, 0.05%; XY, 0.15%).


CONCLUSIONS
This study confirms other previous evaluations on restricted numbers of patients. Our results seem to confirm a relative equiprobability of disomy frequencies concerning the different chromosomal pairs during male meiosis.
Morel
F
F
Unité de Recherche Associée CNRS 561 et Institut d'Etude de Transfert des Gènes, Besançon, France.
Mercier
S
S
Roux
C
C
Clavequin
M C
MC
Bresson
J L
JL
D000328:Adult; D000782:Aneuploidy; D002884:Chromosomes, Human, Pair 15; D002887:Chromosomes, Human, Pair 18; D002898:Chromosomes, Human, Pair 8; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008540:Meiosis; D008875:Middle Aged; D011446:Prospective Studies; D011897:Random Allocation; D012729:Sex Chromosome Aberrations; D013094:Spermatozoa / Q000002:abnormalities* / Q000166:cytology / Q000473:pathology; D014960:X Chromosome; D014998:Y Chromosome