Rat TAFII31 gene is induced upon programmed cell death in differentiated PC12 cells deprived of NGF.
Typical programmed cell death (PCD) requires de novo macromolecular synthesis and shares common morphological changes referred to as apoptosis. To elucidate the molecular mechanism of apoptosis, we isolated cDNA clones that are induced in differentiated PC12 cells deprived of NGF by differential display method. Among such clones, homology searches revealed that the one clone encodes the rat TATA-binding-protein-associated factor TAFII31, a component of TFIID, and a transcriptional coactivator of the p53 protein. Northern analysis of various organs in human showed one band in heart, brain, skeletal muscle and pancreas, whose size is approximately 1.1 kb which identical to that of human TAFII31 mRNA, although the size of rat human TAFII31 mRNA is approximately 2.7 kb. The deduced amino acid sequence of the rat TAFII31 was 77% identical to that of the human TAFII31. Northern analysis of various organs in adult mice showed that expression levels of TAFII31 mRNA were strong in heart but weak in spleen, although this gene is ubiquitously expressed.
Aoki
T
T
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan. tomokazu@kuhp.kyoto-u.ac.jp
Koike
T
T
Nakano
T
T
Shibahara
K
K
Nishimura
H
H
Kikuchi
H
H
Honjo
T
T
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017386:Sequence Homology, Amino Acid; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*; D035362:Transcription Factor TFIID; D015533:Transcriptional Activation
D018076:DNA, Complementary; D009414:Nerve Growth Factors; D012333:RNA, Messenger; C106849:TAF9 protein, human; D035381:TATA-Binding Protein Associated Factors; C491456:Taf9b protein, rat; D015534:Trans-Activators; D035362:Transcription Factor TFIID