Five-membered mercaptoheterocyclic ligands for thiophilic adsorption chromatography.
A series of five-membered mercaptoheterocyles containing at least two heteroatoms in their ring structure was investigated for their ability to specifically adsorb antibodies. The structures that contain at least one double bond adsorbed antibodies, while the saturated structure showed no protein interaction under the experimental conditions studied. The influence of various lyotropic salt on adsorbance was studied.
Schwarz
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BioSepra Inc., MA 01752, USA.
D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D002516:Ceramics / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D002951:Citrates / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008024:Ligands; D012492:Salts / Q000737:chemistry; D000077559:Sodium Citrate; D013431:Sulfates / Q000737:chemistry; D013830:Thiadiazoles / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002951:Citrates; D007074:Immunoglobulin G; D008024:Ligands; D012492:Salts; D000077559:Sodium Citrate; D013431:Sulfates; D013830:Thiadiazoles; D013844:Thiazoles; D000099166:Polyacrylamides; C012036:sodium sulfate