title: Cytomegalovirus monitoring by polymerase chain reaction of whole blood samples from patients undergoing autologous bone marrow or peripheral blood progenitor cell transplantation.
abstract: Sensitive screening for cytomegalovirus (CMV) by polymerase chain reaction (PCR) following autologous bone marrow or peripheral blood progenitor cell transplantation has not been evaluated. In a three-center study, 98 autograft transplant recipients were prospectively screened for CMV infection by PCR and culture techniques. At a median of 20 days (range, 3-28) after transplantation, 21 (39.6%) of 53 CMV-seronegative patients were PCR positive for CMV, and at a median of 17 days (range, 7-84) after transplantation, 19 (42.2%) of 45 CMV-seropositive patients were PCR positive for CMV. Low-level DNAemia (1-10 fg CMV DNA/mL blood) occurred for 1 week in 31 patients but was never associated with CMV disease. Of 9 patients who presented with at least two consecutive positive PCR results, 1 developed CMV pneumonia. No patients died because of CMV disease. Screening for CMV infection by PCR had a negative predictive value of 100% (as also observed after allogeneic transplantation), but its positive predictive value was significantly lower.
authors.affiliation: Medizinische Universitätsklinik, Abteilung II, Kinderklinik, Institut für Medizinische Informationsverarbeitung, and Abteilung für Medizinische Virologie, Universität Tübingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Hebart
authors.forename: A
authors.initials: A
authors.lastname: Schröder
authors.forename: J
authors.initials: J
authors.lastname: Löffler
authors.forename: T
authors.initials: T
authors.lastname: Klingebiel
authors.forename: H
authors.initials: H
authors.lastname: Martin
authors.forename: B
authors.initials: B
authors.lastname: Wassmann
authors.forename: F
authors.initials: F
authors.lastname: Gerneth
authors.forename: H
authors.initials: H
authors.lastname: Rabenau
authors.forename: G
authors.initials: G
authors.lastname: Jahn
authors.forename: L
authors.initials: L
authors.lastname: Kanz
authors.forename: C A
authors.initials: CA
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Einsele
mesh_terms: D000293:Adolescent; D000328:Adult; D001804:Blood Transfusion, Autologous; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000097:blood*; D005347:Fibroblasts; D006801:Humans; D015994:Incidence; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012307:Risk Factors; D019562:Viral Load
chemical_list: D004279:DNA, Viral