title: Antifungal effect of zeolite-incorporated tissue conditioner against Candida albicans growth and/or acid production.
abstract: A new antimicrobial material, Ag-zeolite (Zeomic), was combined with a commercial tissue conditioner (GC-Soft Liner (GC); 1-5%) and, through monitoring the pH of the growth medium, examined for effects on the in vitro growth and/or acid production of Candida albicans on protein-free and saliva-coated specimens. The effect of incorporation of this agent on the physical property of the lining material was also examined according to the ISO penetration test. Comparison studies were carried out using GC, Coe Comfort (CC) or undecylenate combined GC (1-5%) specimens. Although the pH changes in the media varied depending upon the materials on which the Candida was grown, reverse sigmoidal pH curves were observed with most samples. As compared with GC, the soft lining materials showed, to some extent, an inhibitory effect on the acid production and/or the growth of C. albicans. These inhibitory effects consisted of a delay in the onset of rapid pH decline, decreases in the rate of pH change and increases in minimum pH. In most cases, the inhibitory effects of test specimens were dose-dependent, and zeolite specimens showed a significantly higher antifungal effect, followed by CC and undecylenate-combined GC; GC showed the least antifungal effect. The inhibitory effects of these materials on fungal growth were decreased by the presence of a saliva-coat, particularly with zeolite specimens and CC. However, four of eight 5%-Zeomic specimens still exhibited perfect growth inhibition in the presence of the salivary pellicle. Furthermore, test specimens containing 2-5% Zeomic showed a significantly greater effect on the delay in rapid decline of pH, as compared with the other specimens examined. In addition, the significantly higher minimum pH was observed where the yeasts were grown on 4%- and 5%-Zeomic specimens. The physical properties of all the test specimens conformed with the ISO standard as examined by penetration test. These results taken together suggest that an antimicrobial zeolite-combined tissue conditioner would be a potential aid in denture plaque control.
authors.affiliation: Department of Prosthetic Dentistry, Hiroshima University School of Dentistry, Minami-ku, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nikawa
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Hamada
authors.forename: M B
authors.initials: MB
authors.lastname: Rahardjo
authors.forename: H
authors.initials: H
authors.lastname: Murata
authors.forename: S
authors.initials: S
authors.lastname: Nakanoda
mesh_terms: D000143:Acids / Q000378:metabolism; D000180:Acrylic Resins / Q000737:chemistry; D000935:Antifungal Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003470:Culture Media; D044622:Dental Pellicle; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control; D003778:Dentures; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry; D010795:Phthalic Acids / Q000737:chemistry; D012463:Saliva / Q000502:physiology; D012834:Silver; D014016:Tissue Conditioning, Dental; D017641:Zeolites / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000143:Acids; D000180:Acrylic Resins; D000935:Antifungal Agents; D003470:Culture Media; D008689:Methacrylates; D010795:Phthalic Acids; C073909:GC Soft-Liner; D017641:Zeolites; C043685:Coe Comfort; D012834:Silver