The N-terminal Z repeat 5 of connectin/titin binds to the C-terminal region of alpha-actinin.
Connectin/titin, a 3000 kDa protein, links the Z line to the myosin filament in striated muscle sarcomeres. Using the yeast two-hybrid system, the present work shows that the N-terminal Z repeat 5 region (amino acids 447-472) of connectin binds to the C-terminal region (amino acids 825-897) of alpha-actinin, the main constituent of the Z line.
Ohtsuka
H
H
Department of Biology, Faculty of Science, Chiba University, Japan.
Yajima
H
H
Maruyama
K
K
Kimura
S
S
D000185:Actinin / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D003001:Cloning, Molecular; D064211:Connectin; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011494:Protein Kinases / Q000737:chemistry* / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D012518:Sarcomeres / Q000737:chemistry; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D064211:Connectin; D018076:DNA, Complementary; D009124:Muscle Proteins; C577285:TTN protein, human; D000185:Actinin; D011494:Protein Kinases