title: Development of microscopic lesions in splenic cords of pigs infected with African swine fever virus.
abstract: Acute forms of African swine fever are characterized by hemorrhagic lesions in the lymphoid organs. This paper reports the evolution of lesions in the splenic cords of pigs inoculated with African swine fever (ASF) virus (strain Malawi'83). Ultrastructural examination of the splenic cords of the infected pigs revealed numerous macrophages attached to the muscle cells harboring virus replication center and cytopathic effects at 3 dpi (days post-infection). From 5 dpi, the splenic cords contained a large number of erythrocytes associated with abundant fibrin deposits, mainly arranged around the muscle cells, from which macrophages had disappeared. It is likely that the ASF virus replication, and consequent cytopathic effects, observed in the fixed macrophages of splenic cords, may be responsible for the fibrin deposition.
authors.affiliation: Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Cŕdoba, Spain. an1caotl@lucano.uco.es
authors.forename: L
authors.initials: L
authors.lastname: Carrasco
authors.forename: M J
authors.initials: MJ
authors.lastname: Bautista
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Villamandos
authors.forename: J
authors.initials: J
authors.lastname: Martin de las Mulas
authors.forename: F
authors.initials: F
authors.lastname: Chacón-M de Lara
authors.forename: P J
authors.initials: PJ
authors.lastname: Wilkinson
authors.forename: M A
authors.initials: MA
authors.lastname: Sierra
mesh_terms: D000357:African Swine Fever / Q000473:pathology*; D000358:African Swine Fever Virus / Q000302:isolation & purification / Q000502:physiology; D000818:Animals; D003588:Cytopathogenic Effect, Viral; D004912:Erythrocytes / Q000473:pathology / Q000821:virology; D005260:Female; D005337:Fibrin / Q000378:metabolism; D008264:Macrophages / Q000473:pathology / Q000821:virology; D008297:Male; D009130:Muscle, Smooth / Q000473:pathology / Q000821:virology; D013154:Spleen / Q000473:pathology* / Q000821:virology; D013163:Splenomegaly / Q000662:veterinary; D013552:Swine; D014771:Virion / Q000302:isolation & purification / Q000502:physiology; D014779:Virus Replication
chemical_list: D005337:Fibrin