Electrochemiluminescence flow injection immunoassay for atrazine.
Antibodies to atrazine were labelled with glucose oxidase and used in colorimetric enzyme linked immunosorbent assays. Transparent aminosilanized indium tin oxide coated glass electrodes were derivatized with aminodextran covalently modified with atrazine caproic acid. The labelled antibodies were used to investigate the derivatized electrodes colorimetrically and the electrodes were use in an electrochemiluminescence flow injection analyser. Electrochemiluminescence immunoassay for atrazine in the range 0-10 ppb showed that it was possible to detect less than 0.1 ppb, the precautionary limit for pesticides in drinking water recommended by the European Commission.
Wilson
R
R
Department of Chemistry, University of Liverpool, UK.
Barker
M H
MH
Schiffrin
D J
DJ
Abuknesha
R
R
D000911:Antibodies, Monoclonal; D001280:Atrazine / Q000032:analysis*; D003124:Colorimetry; D003911:Dextrans / Q000737:chemistry; D004563:Electrochemistry; D004797:Enzyme-Linked Immunosorbent Assay; D017022:Flow Injection Analysis / Q000379:methods*; D005949:Glucose Oxidase; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D003911:Dextrans; D005949:Glucose Oxidase; D001280:Atrazine