title: Fast specific separation and sensitive quantification of bactericidal and sporicidal aldehydes by high-performance liquid chromatography: example of glutaraldehyde determination.
abstract: This article describes the design and the validation of the HPLC determination of glutaraldehyde at g/l and mg/l concentrations, after derivatization by 2,4-dinitrophenylhydrazine and using the external standard method. At low concentrations, the reaction mixture needs to be heated and a weight ratio of 500 for the 2,4-dinitrophenylhydrazine reagent and the glutaraldehyde ensures a linear calibration curve. In contrast, high concentrations do not require heating of the reaction mixture and a weight ratio of 32 proved to be sufficient. The optimized HPLC method has been validated for both ranges of concentrations. Between 1.25 and 10 mg/l, the content can be determined by the external standard method, with a repeatability of 0.5%. The detection limit is 0.2 mg/l. Between 0.31 and 2.5 g/l, the content can also be determined by the external standard method, with a repeatability of 0.4%. Finally, statistical analysis has demonstrated that aqueous solutions of glutaraldehyde are stable for at least three days at 4 degrees C within the mg to g range.
authors.affiliation: Laboratoire de Chimie Analytique, Faculté de Pharmacie de Paris V, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Menet
authors.forename: D
authors.initials: D
authors.lastname: Gueylard
authors.forename: M H
authors.initials: MH
authors.lastname: Fievet
authors.forename: A
authors.initials: A
authors.lastname: Thuillier
mesh_terms: D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004202:Disinfectants / Q000032:analysis*; D004355:Drug Stability; D005976:Glutaral / Q000032:analysis*; D010659:Phenylhydrazines; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004202:Disinfectants; D010659:Phenylhydrazines; C004787:2,4-dinitrophenylhydrazine; D005976:Glutaral