Caroteno-protein and exopolysaccharide production by co-cultures of Rhodotorula glutinis and Lactobacillus helveticus.
The lactose-negative yeast Rhodotorula glutinis 22P and the homofermentative lactic acid bacterium Lactobacillus helveticus 12A were cultured together in a cheese whey ultrafiltrate containing 42 g L-1 lactose. The chemical composition of the caroteno-protein has been determined. The carotenoid and protein contents are 248 micrograms g-1 dry cells and 48.2% dry weight. Carotenoids produced by Rhodotorula glutinis 22P have been identified as beta-carotene 15%, torulene 10%, and torularhodin 69%. After separating the cell mass from the microbial association, the exopolysaccharides synthesized by Rhodotorula glutinis 22P were isolated from the supernatant medium in a yield of 9.2 g L-1. The monosaccharide composition of the synthesized biopolymer was predominantly D-mannose (57.5%).
Frengova
G
G
Laboratory of Applied Microbiology, Bulgarian Academy of Sciences, Bulgaria.
Simova
E
E
Beshkova
D
D
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000737:chemistry; D002338:Carotenoids / Q000096:biosynthesis*; D003470:Culture Media; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis*; D007778:Lactobacillus / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000096:biosynthesis*; D012248:Rhodotorula / Q000378:metabolism*
D000596:Amino Acids; D002241:Carbohydrates; D003470:Culture Media; D005656:Fungal Proteins; D009005:Monosaccharides; D011134:Polysaccharides; D002338:Carotenoids; D007785:Lactose