Biogenesis of Candida albicans Can1 permease expressed in Saccharomyces cerevisiae.
The Candida albicans CAN1 gene, encoding a high-affinity permease for arginine, lysine and histidine, was tagged at its C-terminus with a c-myc epitope and introduced into strains of Saccharomyces cerevisiae lacking basic amino acid permeases. The expression levels of Ca-Can1p were influenced by the available nitrogen source, being almost negligible when cells were grown in the presence of ammonia. Ca-Can1p was shown to follow the secretory pathway in S. cerevisiae. Ca-Can1p activity was not detected in a secretion-defective sec1-1 mutant grown at a non-permissive temperature. Shr3p, an ER protein that participates in the biogenesis of amino acid permeases was also required for the functional expression of Ca-Can1p. The shr3 mutation does not affect the affinity for substrate but does decrease the number of Can1p molecules reaching the plasma membrane.
Matìjèková
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Department of Membrane Transport, Institute of Physiology, CzAcadSci, Prague, Czech Republic.
Sychrová
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H
D026905:Amino Acid Transport Systems; D000641:Ammonia / Q000378:metabolism / Q000494:pharmacology; D001120:Arginine / Q000378:metabolism; D015153:Blotting, Western; D002176:Candida albicans / Q000201:enzymology*; D002956:Citrulline / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D000939:Epitopes; D005656:Fungal Proteins; D015870:Gene Expression; D006639:Histidine / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010957:Plasmids; D011392:Proline / Q000378:metabolism / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic
D026905:Amino Acid Transport Systems; C097640:CAN1 protein, Candida albicans; D000939:Epitopes; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins; D002956:Citrulline; D006639:Histidine; D000641:Ammonia; D001120:Arginine; D003513:Cycloheximide; D011392:Proline; D008239:Lysine