Detection of MAGE-4 protein in sera of lung cancer patients.
We investigated the level of MAGE-4 protein in sera of patients with primary lung cancer to understand better the biological roles of the MAGE proteins. MAGE-4 protein was detected as a non-degraded form in both the supernatant of a MAGE-4+ tumor cell line and in a patient's serum. Serum level of the MAGE-4 protein in lung cancer patients (n=100, mean=1.17 ng/ml) was significantly higher than that in either patients with benign pulmonary diseases (n=80, 0.33 ng/ml) or healthy donors (n=68, 0.32 ng/ml). It was higher than the cutoff level (1.15 ng/ml) in 34 of 100 cancer patients, but not in anyone in the other groups.
Shichijo
S
S
Department of Immunology, Kurume University School of Medicine.
Hoshino
T
T
Koufuji
K
K
Hayashi
A
A
Kawamoto
M
M
Kikuchi
M
M
Higuchi
T
T
Ichiki
M
M
Oizumi
K
K
Itoh
K
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D008175:Lung Neoplasms / Q000097:blood*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000097:blood*; D010455:Peptides / Q000276:immunology; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; C095204:MAGEA4 protein, human; D009363:Neoplasm Proteins; D010455:Peptides