title: Long-bone biomechanics in mice selected for body conformation.
abstract: Two lines of mice divergently selected from the control strain (CBi) against the positive phenotypic correlation between body weight (b.w.) and tail (skeletal) length were obtained (CBi/C: high weight, short tail; CBi/L: low weight, long tail). The selected animals showed a different relationship between body and skeletal masses. To compare the adequacy between biomass and load-bearing ability of the skeleton, and to describe the eventual role of bone mechanostat in the production of these changes, cross-sectional and bending properties of both femur diaphyses were determined in CBi, CBi/C, and CBi/L adult mice of both genders. Cortical bone material quality (elastic modulus) was reduced in the selected lines (p < 0.001), significantly less in CBi/C than in CBi/L. In contrast, cross-sectional design (b.w.-adjusted values of moment of inertia, CSMI) was largely improved (p < 0.001), significantly more in CBi/C than in CBi/L. These effects determined a greater stiffness and strength in CBi/C than in CBi/L or CBi weight-paired mice. The elevations of the negative regression lines between elastic modulus and CSMI ("distribution/quality" curves) decreased in the order CBi/C > CBi/L > CBi. Data show that selection improved diaphyseal stiffness and strength in CBi/C animals because of an architectural overcompensation for the reduced bone material quality. Therefore, an inadequate control of long-bone architectural design as a function of the mechanical quality of cortical bone and b.w. bearing could have been induced in that line. Assuming bone mechanostatic regulation to be genetically programmed, some of the corresponding biological determinants should be transmitted independently, because artificial selection separately affected material quality and architectural design. The possibility of transmission of an inadequate mechanostatic function (inability to adapt bone modeling to bone material quality as a function of the biomass to be supported) was also shown, as some genotypes could express architectural modifications that largely exceed bone material quality deterioration.
authors.affiliation: Instituto de Genética Experimental, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Santa Fe, Rosario, Argentina.
authors.forename: R J
authors.initials: RJ
authors.lastname: Di Masso
authors.forename: M T
authors.initials: MT
authors.lastname: Font
authors.forename: R F
authors.initials: RF
authors.lastname: Capozza
authors.forename: G
authors.initials: G
authors.lastname: Detarsio
authors.forename: F
authors.initials: F
authors.lastname: Sosa
authors.forename: J L
authors.initials: JL
authors.lastname: Ferretti
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics*; D004548:Elasticity; D005260:Female; D005269:Femur / Q000502:physiology*; D008297:Male; D051379:Mice; D012044:Regression Analysis; D012641:Selection, Genetic; D016474:Weight-Bearing / Q000502:physiology*