Determination of total iodine in biological material by alkaline ashing and column-switching ion-pair liquid chromatography.
A method for the determination of total iodine in biological material has been developed. The method combines alkaline ashing with a selective and sensitive column-switching ion-pair HPLC technique. The ashing procedure which converts the organically bound iodine to iodide is miniaturised and requires only about 100 mg of sample. The first column of the column-switching system is polymer based and can therefore withstand the alkaline pH obtained after ashing. On the analytical column the iodide is separated as an ion-pair with tetrabutyl ammonium hydrogensulphate. The method has been applied to samples from whole blood, urine, liver, lung, carcass, and a sample throughput of at least 50 samples per day can be achieved. Validation studies by spiking experiments showed the precision to be better than 10% R.S.D. for all matrices with recoveries in the range 87-97%. The method has been applied for samples with an iodine content in the range 0.07-1060 microg/g.
Andersson
S
S
Analytical Chemistry Department, Pharmaceutical Development, Pharmacia and Upjohn, Stockholm, Sweden.
Forsman
U
U
D000818:Animals; D001842:Bone and Bones / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006878:Hydroxides; D007455:Iodine / Q000032:analysis* / Q000097:blood / Q000652:urine; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D017680:Potassium Compounds; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012972:Sodium Hydroxide; D013056:Spectrophotometry, Ultraviolet; D014018:Tissue Distribution
D006878:Hydroxides; D017680:Potassium Compounds; D012972:Sodium Hydroxide; D007455:Iodine; C029943:potassium hydroxide