Expression of the proliferation-related Ki-67 mRNA in the early development of murine embryo.
In a search for early lymphoid-specific genes, we isolated a cDNA clone (LL7) encoding a murine homologue of Ki-67 protein, a proliferation-related nuclear antigen. LL7 transcript appears preferentially in lymphoid organs as the bone marrow, spleen, and the thymus. Here, we studied the expression of murine Ki-67 (mKi-67) mRNA among various organs or tissues during the early development of fetus. In fetus, mKi-67 mRNA appears developmentally as early as day 11 and is expressed maximally at day 15. In situ hybridization on the section revealed that the expression of mKi-67 mRNA is preferential in the area of active organ formation such as neurological system and the fetal liver. These results suggest that mKi-67 plays an important role in the proliferation of early embryonic precursor cells of neurological and immune systems.
Mitsuyoshi
S
S
Department of Immunology, Kumamoto University School of Medicine, Honjo, Japan.
Igarashi
H
H
Sakata
A
A
Koseki
H
H
Taniguchi
M
M
Sakaguchi
N
N
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism*; D005314:Embryonic and Fetal Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D019394:Ki-67 Antigen / Q000235:genetics*; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008221:Lymphoid Tissue / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000235:genetics; D051757:PAX5 Transcription Factor; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism; D014157:Transcription Factors; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D051757:PAX5 Transcription Factor; C497282:Pax5 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors