Expression and mutations of p53 in salivary gland tumours.
A series of 219 salivary gland tumours (103 carcinomas and 116 benign tumours) were analysed for p53 protein expression using immunohistochemistry, and for mutations in p53 gene using non-radioactive single strand conformation polymorphism (SSCP). p53 expression was present in 36% (42/116) of the benign tumours and in 54% (56/103) of the carcinomas. The highest prevalence of p53 expression was found in adenoid cystic carcinomas (69%), followed by mucoepidermoid carcinomas (67%). Of the benign tumours, pleomorphic adenomas showed the highest prevalence of p53 positivity (41%). In malignant tumours, expression of p53 bore no correlation to local recurrence, metastatic disease or survival of the patients. Exons 5 through 9 were analysed and four mutations were found in 20 cases of p53-immunopositive tumours and two in 20 p53-negative tumours. Each of the exons 5, 6 and 8/9 had two mutations, whereas no mutations were detected in exon 7.
Kärjä
V J
VJ
Department of Pathology, University of Kuopio, Finland.
Syrjänen
K J
KJ
Kurvinen
A K
AK
Syrjänen
S M
SM
D008949:Adenoma, Pleomorphic / Q000737:chemistry / Q000235:genetics; D000911:Antibodies, Monoclonal; D003528:Carcinoma, Adenoid Cystic / Q000737:chemistry / Q000235:genetics; D018277:Carcinoma, Mucoepidermoid / Q000737:chemistry / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000235:genetics; D016009:Chi-Square Distribution; D004252:DNA Mutational Analysis; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D012468:Salivary Gland Neoplasms / Q000737:chemistry* / Q000235:genetics; D016019:Survival Analysis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
D000911:Antibodies, Monoclonal; D016159:Tumor Suppressor Protein p53