Expression cloning and intracellular localization of a human ZF5 homologue.
We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.
Sugiura
K
K
Department of Anatomy, Nagoya University School of Medicine, Showa-ku, Japan.
Muro
Y
Y
Nagai
Y
Y
Kamimoto
T
T
Wakabayashi
T
T
Ohashi
M
M
Hagiwara
M
M
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry* / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D051741:Kruppel-Like Transcription Factors; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics*
C093752:Bric-a-brac protein 1, Drosophila; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D007106:Immune Sera; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; D014157:Transcription Factors; C489978:ZBTB14 protein, human