title: Allergen-induced recruitment of Fc epsilon RI+ eosinophils in human atopic skin.
abstract: We have attempted to identify Fc epsilon RI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30% expressed mRNA for the alpha, beta, and gamma chains of Fc epsilon RI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-alpha chain antibody, the Fc epsilon RI alpha protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of Fc epsilon RI+ (997+)/EG2+ eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express Fc epsilon. RI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.
authors.affiliation: Allergy and Clinical Immunology, Imperial College School of Medicine at the National Heart & Lung Institute, London, GB.
authors.forename: L T
authors.initials: LT
authors.lastname: Barata
authors.forename: S
authors.initials: S
authors.lastname: Ying
authors.forename: J A
authors.initials: JA
authors.lastname: Grant
authors.forename: M
authors.initials: M
authors.lastname: Humbert
authors.forename: J
authors.initials: J
authors.lastname: Barkans
authors.forename: Q
authors.initials: Q
authors.lastname: Meng
authors.forename: S R
authors.initials: SR
authors.lastname: Durham
authors.forename: A B
authors.initials: AB
authors.lastname: Kay
mesh_terms: D000485:Allergens / Q000502:physiology*; D002465:Cell Movement / Q000276:immunology*; D004396:Coloring Agents; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D004804:Eosinophils / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009282:Naphthalenesulfonates; D017455:Receptors, IgE / Q000032:analysis*; D013194:Staining and Labeling
chemical_list: D000485:Allergens; D004396:Coloring Agents; D009282:Naphthalenesulfonates; D017455:Receptors, IgE; C070620:chromotrope 2R