In vivo endoscopic optical biopsy with optical coherence tomography.
Current medical imaging technologies allow visualization of tissue anatomy in the human body at resolutions ranging from 100 micrometers to 1 millimeter. These technologies are generally not sensitive enough to detect early-stage tissue abnormalities associated with diseases such as cancer and atherosclerosis, which require micrometer-scale resolution. Here, optical coherence tomography was adapted to allow high-speed visualization of tissue in a living animal with a catheter-endoscope 1 millimeter in diameter. This method, referred to as "optical biopsy," was used to obtain cross-sectional images of the rabbit gastrointestinal and respiratory tracts at 10-micrometer resolution.
Tearney
G J
GJ
Department of Electrical Engineering and Computer Science, Building 36-357, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Brezinski
M E
ME
Bouma
B E
BE
Boppart
S A
SA
Pitris
C
C
Southern
J F
JF
Fujimoto
J G
JG
D019412:Anatomy, Cross-Sectional; D000818:Animals; D001706:Biopsy; D002404:Catheterization / Q000295:instrumentation; D019723:Endoscopes; D004848:Epithelium / Q000033:anatomy & histology; D020701:Esophagoscopes; D004947:Esophagus / Q000033:anatomy & histology* / Q000098:blood supply; D005336:Fiber Optic Technology; D007368:Interferometry / Q000295:instrumentation; D007834:Lasers; D009092:Mucous Membrane / Q000033:anatomy & histology; D011817:Rabbits; D012542:Scattering, Radiation; D014054:Tomography / Q000295:instrumentation / Q000379:methods*; D014132:Trachea / Q000033:anatomy & histology*