Amperometric immunosensors based on rigid conducting immunocomposites.
Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgC1). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay.
Santandreu
M
M
Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
Céspedes
F
F
Alegret
S
S
Martínez-Fàbregas
E
E
D000818:Animals; D001667:Binding, Competitive; D001672:Biocompatible Materials; D015374:Biosensing Techniques; D003188:Composite Resins / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D008689:Methacrylates / Q000737:chemistry; D010755:Organophosphates / Q000378:metabolism; D011817:Rabbits; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry
D001672:Biocompatible Materials; D003188:Composite Resins; D004852:Epoxy Compounds; D007074:Immunoglobulin G; D008689:Methacrylates; D010755:Organophosphates; D018030:Silver Compounds; D012834:Silver; C074782:phenylphosphate; D006108:Graphite; C037548:silver chloride