Rapid separation of soybean globulins by reversed-phase high-performance liquid chromatography.
A rapid separation of the main soybean proteins (7S and 11S globulins) was carried out by reversed-phase high-performance liquid chromatography. For this purpose, a linear binary gradient acetonitrile--water--0.1% trifluoroacetic acid, at a flow-rate of 1 ml/min and 50 degrees C temperature was designed. Under the experimental conditions of this work, it was possible to separate five peaks corresponding to the globulins from a soybean protein isolate in 9 min. The characterization of soybean proteins was accomplished by analyzing the 7S and 11S purified fractions obtained from a soybean protein isolate. The method was applied to the separation of soybean proteins from commercial foodstuffs: soybean flour, textured soybean and soybean milks.
García
M C
MC
Centro de Tecnología de los Alimentos, Universidad de Alcalá de Hernares, Madrid, Spain.
Torre
M
M
Laborda
F
F
Marina
M L
ML
D002851:Chromatography, High Pressure Liquid; D005433:Flour / Q000032:analysis; D005526:Food, Formulated / Q000032:analysis*; D005916:Globulins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D030262:Soybean Proteins / Q000737:chemistry*; D013696:Temperature
D005916:Globulins; D030262:Soybean Proteins