Sodium butyrate decreases histamine-stimulated calcium mobilization in C6 glioma cells.
The aim of this study was to investigate the effect of sodium butyrate on calcium mobilization. Histamine was found to stimulate a dose-dependent increase in intracellular calcium concentrations ([Ca2+]i) through H1 receptors, but this effect was attenuated in C6 cells pretreated with 1-5 mM sodium butyrate. Evidence is provided that release of Ca2+ from intracellular stores is decreased in a dose-dependent manner. Experiments with BAPTA that show lower levels of [Ca2+]i in cells pretreated with higher concentrations of sodium butyrate suggest that sodium butyrate also decreases Ca2+ influx. These results suggest that changes in Ca2+ mobilization are at least partially responsible for sodium butylate-induced C6 cell differentiation.
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Institute of Neuroscience and Department of Physiology, National Yang Ming University, Taipei, Taiwan, ROC.
Jea-Chien
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Sun
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D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002744:Chlorpheniramine / Q000494:pharmacology; D005456:Fluorescent Dyes; D016257:Fura-2; D005910:Glioma / Q000378:metabolism*; D006633:Histamine Antagonists / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D002087:Butyrates; D005456:Fluorescent Dyes; D006633:Histamine Antagonists; D006634:Histamine H1 Antagonists; D020148:Butyric Acid; D002744:Chlorpheniramine; D002118:Calcium; D016257:Fura-2