The effect of ultraviolet B induced adult T cell leukemia-derived factor/thioredoxin (ADF/TRX) on survival and growth of human melanocytes.
Ultraviolet B (UVB) radiation is known to induce reactive oxygen species (ROS) in the skin. The skin, however, counteracts ROS by both constitutional and newly produced antioxidants. One such antioxidant, adult T cell leukemia-derived factor (ADF), a human homologue of thioredoxin (TRX), was shown to be efficiently produced in and released from cultured normal human keratinocytes after UVB irradiation by Northern and Western blot analyses and enzyme-linked immunoabsorbent assay (ELISA). Recombinant ADF (rADF) did not rescue UVB-induced melanocyte death, either when added pre- or post-UV irradiation. However, further addition of neutralizing antibody caused cell death of both keratinocytes and melanocytes. rADF was shown to induce higher expression in melanocortin-1 receptor (MC1-R) mRNA accompanied by increased binding activity using 125I labeled [Nle4, D-Phe7]-alpha-MSH in melanocytes, leading to the enhanced increment of DNA synthesis. Taken together, it was shown that released ADF from UVB-irradiated keratinocytes acts as a survival factor for both keratinocytes and melanocytes but does not rescue UV-induced melanocyte death. Further, it may work as one of the stimulatory factors for UVB-induced melanogenesis by upregulating MSH-R binding activity in combination with the enhanced DNA synthesis by alpha-MSH.
Funasaka
Y
Y
Department of Dermatology, Kobe University School of Medicine, Japan. funasaka@med.kobe-u.ac.jp
Ichihashi
M
M
D000293:Adolescent; D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000096:biosynthesis / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D011979:Receptors, Pituitary Hormone / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013879:Thioredoxins / Q000096:biosynthesis / Q000494:pharmacology*; D014466:Ultraviolet Rays
D016207:Cytokines; D006133:Growth Substances; D009363:Neoplasm Proteins; D011979:Receptors, Pituitary Hormone; D011994:Recombinant Proteins; C055533:adult T cell leukemia-derived factor; D013879:Thioredoxins; C029446:MSH receptor; D004247:DNA