Expanded-bed adsorption in industrial bioprocessing: recent developments.
Expanded-bed adsorption allows the capture of proteins from particle-containing feedstocks without prior removal of particulates, thus enabling clarification of a cell suspension or cell homogenate and the concentration of the desired product in a single operation. This usually results in higher product recovery in a shorter time period. Process development and scale-up of an expanded-bed operation is convenient because both the adsorbent and the equipment are similar to those in conventional packed-bed chromatography. This article reviews the recent developments in expanded-bed adsorption technology and the range of applications that are now being achieved.
Hjorth
R
R
Pharmacia Biotech AB, Uppsala, Sweden. rolf.hjorth@eu.pharmacia.com
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification*; D018929:Cell Culture Techniques / Q000379:methods*; D005656:Fungal Proteins / Q000302:isolation & purification*; D007218:Industrial Microbiology / Q000639:trends*
D001426:Bacterial Proteins; D005656:Fungal Proteins