Solution conformation of alpha, beta or gamma-methylglutamyl-containing derivatives as probes of vitamin K-dependent carboxylase using molecular modelling and nuclear magnetic resonance.
In the present study, the conformational behaviour of methyl substituted N-BOC glutamic acid methyl esters (2M, 3T, 3E, 4T, 4E) has been completely characterized through combined NMR and molecular modeling studies. Hetero- and homonuclear coupling constants were measured in order to assign the remaining diastereotopic methylene protons at C(3) and/or C(4), and used for comparison with theoretical data. In parallel, the complete conformational analysis of these analogues has been achieved using molecular mechanics and molecular dynamics (MD) methods. The conformation of the glutamyl residue is established by the excellent agreement between the experimental and calculated side chain scalar coupling constants. The theoretical NMR data were calculated taking into account all the accessible conformations and using the averaging methods appropriate for internal motions. There is a significant influence of the methyl group on the conformational behaviour and on the biological relevance of these structures. Steric effect or electrostatic interaction may also have a considerable influence in stabilizing a conformational population in D2O solution. The conformational preferences of those different analogues in aqueous and methanol solution are discussed in the light of biological results obtained on the vitamin K-dependent carboxylase system.
Larue
V
V
Université René Descartes-Paris V. Laboratoire de Chimie et Biochimie Pharmacologique et Toxicologique (URA 400 CNRS), France.
Gharbi-Benarous
J
J
Acher
F
F
Arulmozhi
V
V
Tisné
C
C
Todeschi
N
N
Azerad
R
R
Girault
J P
JP
D001665:Binding Sites; D019735:Carbon-Carbon Ligases; D005971:Glutamates / Q000737:chemistry; D008025:Ligases / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D015335:Molecular Probes / Q000737:chemistry; D015394:Molecular Structure; D012996:Solutions; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D005971:Glutamates; D046911:Macromolecular Substances; D015335:Molecular Probes; D012996:Solutions; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase