Structural analysis of a bovine arginine tRNA(CCG) gene.
A bovine genomic clone containing a 17.4-kb DNA fragment was isolated and found to contain a solitary arginine tRNA gene with an anticodon of CCG that has a 100% identity to its cognate tRNA. This arginine tRNA gene, symbolized as TRR4, has a characteristic internal split promoter and a typical termination site for RNA polymerase III. The tRNA gene was transcribed in vitro by RNA polymerase III using a HeLa cell-free extract to yield a mature-sized tRNA product. The gene was mapped to bovine chromosome 19 using a panel of bovine-rodent somatic cell hybrid DNAs.
Eubanks
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Department of Biological Sciences, University of North Texas, Denton 76203, USA.
Roeder
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MJ
Pirtle
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Pirtle
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RM
D000818:Animals; D000926:Anticodon; D001120:Arginine / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D012343:RNA, Transfer / Q000235:genetics*
D000926:Anticodon; D004247:DNA; D012343:RNA, Transfer; D001120:Arginine