PF1092A, B and C, new nonsteroidal progesterone receptor ligands produced by Penicillium oblatum. I. Taxonomy of producing strain, fermentation, isolation and biological activities.
Three new nonsteroidal progesterone receptor ligands, PF1092A, B and C, have been isolated from Penicillium oblatum. They were purified from the solid cultures of rice media using ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatographies, and crystallization. All three ligands competitively inhibited [3H]-progesterone binding to porcine uteri cytosol preparations with IC50 of 3.0 x 10 nM (PF1092A), 2.2 x 10(2) nM (PF1092B) and 2.2 x 10(3) nM (PF1092C).
Tabata
Y
Y
Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd., Yokohama, Japan.
Miike
N
N
Hatsu
M
M
Kurata
Y
Y
Yaguchi
T
T
Someya
A
A
Miyadoh
S
S
Hoshiko
S
S
Tsuruoka
T
T
Omoto
S
S
D000818:Animals; D001667:Binding, Competitive; D003600:Cytosol / Q000378:metabolism; D005285:Fermentation; D005663:Furans / Q000302:isolation & purification* / Q000494:pharmacology; D008024:Ligands; D009284:Naphthols / Q000302:isolation & purification* / Q000494:pharmacology; D010407:Penicillium; D011374:Progesterone / Q000378:metabolism; D011980:Receptors, Progesterone / Q000187:drug effects*; D012717:Sesquiterpenes / Q000302:isolation & purification* / Q000494:pharmacology; D013552:Swine
D005663:Furans; D008024:Ligands; D009284:Naphthols; C106748:PF 1092A; C106749:PF 1092B; C106750:PF 1092C; D011980:Receptors, Progesterone; D012717:Sesquiterpenes; D011374:Progesterone