Purification and cDNA cloning of maize HMGd reveal a novel plant chromosomal HMG-box protein with sequence similarity to HMGa.
We have purified the chromosomal high mobility group (HMG) protein HMGd from maize suspension culture cells, determined the N-terminal amino acid (aa) sequence, and isolated the corresponding cDNA. Sequence analysis showed that the cDNA encoded a protein of 126 aa residues with a theoretical mass of 14,104 Da. The protein contains an HMG-box DNA-binding domain and a short acidic C-terminal tail. HMGd is in approx. 65% of its residues identical to maize HMGa, whereas it is only approx. 46% identical to maize HMGcl/2. The differences to the previously reported HMG proteins in aa sequence, in overall charge and in protein size indicate that we have identified a third type of plant chromosomal HMG-box protein belonging to the HMG1 protein family. Immunoblot analysis with a HMGd antiserum reveals that HMGd is expressed in all tissues tested.
Grasser
K D
KD
Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, Germany. grasser@maize.biologie.uni-freiburg.de
Grimm
R
R
Igloi
G L
GL
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D029721:Drosophila Proteins; D018506:Gene Expression Regulation, Plant; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D029721:Drosophila Proteins; C106684:HMGa protein, Zea mays; D006609:High Mobility Group Proteins; C106685:HmgD protein, Drosophila; D010940:Plant Proteins