title: A case study and use of sedimentation equilibrium analytical ultracentrifugation as a tool for biopharmaceutical development.
abstract: Analytical ultracentrifugation (AUC) has reemerged as a powerful technique for protein characterisation. We report the pivotal role sedimentation equilibrium AUC has played in the development of macrophage inflammatory protein-1 alpha (MIP-1 alpha) as a protein therapeutic. MIP-1 alpha has potential clinical applications in cancer but its clinical use is limited, since it associates to form large insoluble aggregates in physiological buffers. Using AUC as a screening technique, we have produced a biologically active variant of MIP-1 alpha, BB-10010, which has a reduced tendency to aggregate in physiological buffers. The aggregation of protein based pharmaceuticals is routinely monitored by size exclusion chromatography (SEC). Comparison of the data acquired by SEC and AUC, demonstrates that owing to the complexity of BB-10010, AUC analysis is required in addition to SEC to provide a rigorous characterisation of molecular association. This work has been extended to include the use of AUC as an analytical tool to monitor the quality of BB-10010 during formulation and stability studies.
authors.affiliation: British Biotech Pharmaceuticals Ltd. Oxford, UK.
authors.forename: P G
authors.initials: PG
authors.lastname: Varley
authors.forename: A J
authors.initials: AJ
authors.lastname: Brown
authors.forename: H C
authors.initials: HC
authors.lastname: Dawkes
authors.forename: N R
authors.initials: NR
authors.lastname: Burns
mesh_terms: D001702:Biopharmaceutics / Q000379:methods*; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D015195:Drug Design; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis / Q000737:chemistry*; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D009994:Osmolar Concentration; D011485:Protein Binding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012441:Saccharomyces cerevisiae; D014461:Ultracentrifugation / Q000379:methods*
chemical_list: D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D011994:Recombinant Proteins