title: Production of alpha-terpineol from Escherichia coli cells expressing thermostable limonene hydratase.
abstract: The genes encoding a thermostable limonene hydratase have been located on a cloned fragment in Escherichia coli conferring growth on limonene and production of the monoterpenes perillyl alcohol and alpha-terpineol. Whole cell bioconversion studies at elevated temperature employing both an aqueous phase and neat limonene phase demonstrated significant production of alpha-terpineol with additional production of carvone.
authors.affiliation: Department of Microbiology, Michigan State University, East Lansing 48824, USA.
authors.forename: N
authors.initials: N
authors.lastname: Savithiry
authors.forename: T K
authors.initials: TK
authors.lastname: Cheong
authors.forename: P
authors.initials: P
authors.lastname: Oriel
mesh_terms: D019149:Bioreactors; D003001:Cloning, Molecular; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D005798:Genes, Bacterial; D001411:Geobacillus stearothermophilus / Q000235:genetics / Q000378:metabolism; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism*; D000077222:Limonene; D039821:Monoterpenes; D013696:Temperature; D013729:Terpenes / Q000378:metabolism*
chemical_list: D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D013729:Terpenes; C016775:alpha-terpineol; C006923:carvone; D000077222:Limonene; D006836:Hydro-Lyases; C106636:limonene hydratase