Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila.
A gene capable of conferring spectinomycin resistance was isolated from Legionella pneumophila, the agent of Legionnaires' disease. The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases. Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demonstrated resistance to spectinomycin, and Southern hybridizations determined that aph also exists in other legionellae.
Suter
T M
TM
Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611, USA.
Viswanathan
V K
VK
Cianciotto
N P
NP
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D016952:Legionella pneumophila / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D000198:Spectinomycin / Q000378:metabolism* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D000198:Spectinomycin; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C106858:spectinomycin phosphotransferase