title: Studies on oriented and reversible immobilization of glycoprotein using novel boronate affinity gel.
abstract: The authors have previously synthesized a novel boronate affinity ligand, catechol [2-(diethylamino)carbonyl-4-bromomethyl]phenylboronate. When this ligand was coupled to cellulose beads, it bound horseradish peroxidase (HRP), a glycoprotein, at pH 7.0. In comparison, commercial m-aminophenylboronic acid-agarose did not bind HRP below pH 8.0. HRP was immobilized in an oriented and reversible fashion using this gel. The immobilized enzyme retained 90.12 per cent of its original activity, probably due to its attachment via the carbohydrate moiety of the enzyme. After repeated use, the activity remaining on the new gel was twice as high as that on conventional m-aminophenylboronic acid-agarose. The column was regenerated easily by washing with dilute acid because of reversibility of the boronate glycol bond.
authors.affiliation: Biotechnology Center, Utah State University, Logan 84322-4700, USA.
authors.forename: X C
authors.initials: XC
authors.lastname: Liu
authors.forename: W H
authors.initials: WH
authors.lastname: Scouten
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D001897:Boronic Acids / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D004800:Enzymes, Immobilized; D005782:Gels; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure
chemical_list: D001896:Boron Compounds; D001897:Boronic Acids; D004800:Enzymes, Immobilized; D005782:Gels; D006023:Glycoproteins; C107009:catechol-(2-(diethylamino)carbonyl-4-bromomethyl)phenylboronate; D006735:Horseradish Peroxidase; C010686:benzeneboronic acid