title: Interleukin-17 up-regulation of nitric oxide production in human osteoarthritis cartilage.
abstract: OBJECTIVE
  To examine the effect of human interleukin-17 (IL-17) on nitric oxide (NO) production in human osteoarthritis (OA) cartilage under ex vivo conditions.
  
  
  METHODS
  OA cartilage from patients undergoing knee replacement surgery was used in explant assays to assess the effect of IL-17. NO production was measured by estimating the stable NO metabolite, nitrite, in conditioned medium.
  
  
  RESULTS
  IL-17 augmented the spontaneous production of nitric oxide. This augmentation was sensitive to cycloheximide and pyrrolidine dithiocarbamate, but not to dexamethasone or soluble IL-1 receptor.
  
  
  CONCLUSIONS
  IL-17 augments nitric oxide production in OA cartilage via nuclear factor kappaB activation, but independently of IL-1beta signaling.
authors.affiliation: Hospital for Joint Diseases, New York, New York 10003, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Attur
authors.forename: R N
authors.initials: RN
authors.lastname: Patel
authors.forename: S B
authors.initials: SB
authors.lastname: Abramson
authors.forename: A R
authors.initials: AR
authors.lastname: Amin
mesh_terms: D000368:Aged; D002358:Cartilage, Articular / Q000378:metabolism*; D016207:Cytokines / Q000502:physiology; D006801:Humans; D020381:Interleukin-17; D007378:Interleukins / Q000502:physiology*; D007719:Knee Joint; D007720:Knee Prosthesis; D008875:Middle Aged; D009569:Nitric Oxide / Q000096:biosynthesis*; D010003:Osteoarthritis / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D007378:Interleukins; D009569:Nitric Oxide