Clinical and epidemiological correlations between the infection with HPV 16 and HPV 18 and female cervical lesions.
A number of 66 specimens from female cervical lesions were examined for infection with human papillomavirus (HPV) types 6, 11, 16, and 18 by nucleic acid hybridization in dot-blot techniques and 35 sera were tested by the immunodot-blot technique, in order to detect the presence of anti E4 and E7 HPV protein antibodies. The findings were compared with the histologic diagnosis. Fifty-six per cent of specimens contained HPV DNA sequences. In 47% of specimens from cervical carcinoma, HPV 11 was detected in 4 cases, HPV 16 in 21 cases, and HPV 18 in 7 cases. Serum antibodies against HPV 16 E4 and HPV 16 E7 occurred in all the cases of uterine carcinoma, in 4 of 10 cases of CIN I-II, and in 3 of 5 sera obtained from apparently healthy women. The analysis of risk factors disclosed the early onset of sexual activity, a relatively high number of births and abortions before the age of 22 years, the use of oral oestroprogestative contraceptive agents, the presence in anamnesis of genital infections with bacterial flora--Candida albicans, Trichomonas vaginalis, Chlamydia trachomatis, Mycoplasma, etc. Our results showed that HPV typing by nucleic acid hybridization was useful for differentiating low- from high-risk cervical lesions and also tried to elucidate the risk factors associated with HPV infections and progression to malignancy.
Stoian
M
M
Stefan S. Nicolau Institute of Virology.
Repanovici
R
R
Corniţescu
F
F
D000028:Abortion, Induced; D000328:Adult; D000368:Aged; D002181:Candidiasis, Vulvovaginal / Q000453:epidemiology; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology*; D002690:Chlamydia Infections / Q000150:complications; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000453:epidemiology; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology*; D011247:Pregnancy; D012016:Reference Values; D012307:Risk Factors; D012725:Sexual Behavior; D014247:Trichomonas Vaginitis / Q000453:epidemiology; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000453:epidemiology*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000601:surgery / Q000821:virology*; D014594:Uterine Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000601:surgery / Q000821:virology*
D004279:DNA, Viral