Centric rings, acentric rings and excess acentric fragments based on a random-walk interphase chromosome model.
Excess acentric fragments, consisting of acentric rings and acentric linear fragments, are among the most frequent kinds of chromosome-type aberrations produced by radiation. The frequency of acentric rings cannot be obtained directly by experiment but is estimated here from the ratio of acentric to centric rings, evaluated using a random-walk model for the organization of chromatin during interphase and an assumption that the probability of an exchange formation is proportional to the rate of collision between two DSB. This ratio is calculated to be 2.5 in low-LET irradiated human fibroblasts, significantly greater than the ratio if proximity effects are not considered. The calculated frequency of acentric rings is insufficient to account for all the observed excess acentric fragments. Assuming that the rest of the excess acentric fragments are due to incomplete exchanges, all possible recombinations between two DSB that result in acentric rings and acentric linear fragments have been identified. From the chromosome aberration data, the incompleteness parameter has been estimated. Intra-arm chromosome exchanges, either complete or incomplete, were estimated to account for more than 50% of the excess acentric fragments in human fibroblasts.
Wu
H
H
NASA Johnson Space Center, Radiation Biophysics Laboratory, Houston Texas, USA.
Durante
M
M
Sachs
R K
RK
Yang
T C
TC
D002869:Chromosome Aberrations; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D018499:Linear Energy Transfer