Mucosal immune response to an HIV C4/V3 peptide following nasal or intestinal immunization of rabbits.
The HIV env-encoded synthetic peptide T1-SP10MN(A) contains immunodominant epitopes of the C4/V3 regions of gp120. The mucosal immunogenicity of this peptide in various vaccine preparations was first tested in rabbits using chronically isolated Thiry-Vella (T-V) ileal loops. Intestinal and serum samples collected from rabbits immunized via T-V loops demonstrated secretory IgA (S-IgA) and IgG anti-T1-SP10MN(A), respectively, when assayed by ELISA. Intranasal delivery of the peptide supplemented with cholera toxin (CT) resulted in serum IgG and S-IgA anti-T1-SP10MN(A) in vaginal and nasal secretions. This study further demonstrates the utility of rabbits as a convenient animal model for HIV vaccine research and the relationship between nasal immunization and vaginal immunity.
Winchell
J M
JM
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269, USA.
Van Kruiningen
H J
HJ
Silbart
L K
LK
D016915:AIDS Vaccines / Q000008:administration & dosage; D000281:Administration, Intranasal; D000818:Animals; D002772:Cholera Toxin / Q000008:administration & dosage; D005260:Female; D015483:HIV Antibodies / Q000096:biosynthesis / Q000097:blood; D015699:HIV Envelope Protein gp120 / Q000008:administration & dosage / Q000276:immunology*; D018928:Immunity, Mucosal; D007114:Immunization; D016056:Immunodominant Epitopes / Q000008:administration & dosage; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis / Q000097:blood; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007413:Intestinal Mucosa / Q000276:immunology; D009297:Nasal Mucosa / Q000276:immunology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology*; D011817:Rabbits; D014621:Vagina / Q000276:immunology
D016915:AIDS Vaccines; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D016056:Immunodominant Epitopes; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D010446:Peptide Fragments; D002772:Cholera Toxin