A comparison of human immunodeficiency virus type 1 inhibition by partially purified aqueous extracts of Chinese medicinal herbs.
A multiple screening approach to detect compounds inhibitory to various aspects of the human immunodeficiency virus type 1 (HIV-1) life-cycle has been applied to aqueous extracts of 19 herbs traditionally used in Chinese medicine as anti-viral agents. The extracts were tested for their ability to inhibit HIV-1 in a series of in vitro assays. The extracts were tested for inhibition of the interaction between HIV-1 gp120 and immobilized CD4 receptor, inhibition of recombinant HIV-1 reverse transcriptase and for inhibition of three glycohydrolase enzymes that contribute to viral protein glycosylation. Six of the herb extracts (30%) were potent inhibitors of the interaction between HIV-1 gp120 and the CD4 receptor (ID50 5.6 - 79.4 microg/ml), two extracts (10%) contained potent reverse transcriptase inhibitors (ID50 16.9 - 26.0 microg/ml) and 14 extracts (75%) were able to inhibit at least one of the glycohydrolase enzymes.
Collins
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Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories. racollins@cuhk.edu.hk
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Fong
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Wan
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Yeung
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D015704:CD4 Antigens / Q000187:drug effects / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors; D015699:HIV Envelope Protein gp120 / Q000187:drug effects / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology
D015704:CD4 Antigens; D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D015699:HIV Envelope Protein gp120; D018894:Reverse Transcriptase Inhibitors; D006026:Glycoside Hydrolases