Toxoplasma gondii recombinant antigens H4 and H11: use in ELISAs for detection of toxoplasmosis in swine.
Toxoplasma gondii recombinant antigens H4 and H11 were assessed for their potential for use in ELISA for diagnosis of toxoplasmosis in swine. The antigens were evaluated with sera from young pigs experimentally infected with T. gondii. Results were compared with ELISAs based on a native T. gondii antigen extract. Although recombinant antigen ELISAs showed a sharp rise in response with some sera very early after infection, they were relatively non-reactive with late (chronic) infection sera.
Andrews
C D
CD
USDA, Food Safety and Inspection Service, Athens, GA 30605, USA. AndrewsCD@aol.com
Dubey
J P
JP
Tenter
A M
AM
Webert
D W
DW
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D011994:Recombinant Proteins