Transfusing red blood cells stored in citrate phosphate dextrose adenine-1 for 28 days fails to improve tissue oxygenation in rats.
OBJECTIVE
To determine whether the time that red blood cells are stored in citrate phosphate dextrose adenine-1 solution before transfusion alters the ability to improve tissue oxygenation.


METHODS
Prospective, randomized, controlled study.


METHODS
University research institute laboratory.


METHODS
Male Sprague-Dawley rats (350 to 450 g).


METHODS
Twenty-four hours after randomization to sham laparotomy (n = 21) or cecal ligation and perforation (n = 16)1 supply-dependency of systemic oxygen uptake (VO2) was induced in rats by isovolemic hemodilution. Rats were then re-randomized to receive either rat red blood cells stored in citrate phosphate dextrose adenine-1 for 3 days ("fresh" n = 17) or rat red blood cells stored in citrate phosphate dextrose adenine-1 for 28 days ("old" n = 20).


RESULTS
Changes in systemic VO2 were measured for 90 mins to determine the efficiacy of the treatment. Statistical analysis included a fully factorial repeated-measures, generalized linear model. No significant interaction was found between cecal ligation and perforation or sham animals and transfusion with fresh or old red blood cells. However, comparing the combined groups of animals receiving either fresh or old red blood cells, we found that after the transfusion of old red blood cells, systemic VO2 was not significantly improved (after hemodilution 1.68 +/- 0.27 mL/100 g/min, after transfusion 1.86 +/- 0.17 mL/100 g/min; p > .05). In contrast, transfusion with fresh red blood cells acutely increased systemic VO2 (after hemodilution 1.62 +/- 0.06 mL/100 g/min, after transfusion 2.10 +/- 0.09 mL/100 g/min; p = .049).


CONCLUSIONS
Storage of rat red blood cells for 28 days in citrate phosphate dextrose adenine-1 impaired their ability to improve tissue oxygenation when transfused into either control or septic rats placed into supply dependency of systemic VO2.
Fitzgerald
R D
RD
A. C. Burton Vascular Biology Laboratory, Victoria Hospital Research Institute, University of Western Ontario, London, Canada.
Martin
C M
CM
Dietz
G E
GE
Doig
G S
GS
Potter
R F
RF
Sibbald
W J
WJ
D000225:Adenine; D000818:Animals; D000925:Anticoagulants; D001793:Blood Preservation / Q000009:adverse effects* / Q000379:methods; D002951:Citrates; D017707:Erythrocyte Transfusion; D005947:Glucose; D006438:Hemodilution; D006439:Hemodynamics; D008026:Ligation; D008297:Male; D010101:Oxygen Consumption; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000209:etiology; D013997:Time Factors
D000925:Anticoagulants; D002951:Citrates; C007077:citrate phosphate dextrose; D005947:Glucose; D000225:Adenine