Genetic evidence for a clonal link between low and high-grade components in gastric MALT B-cell lymphoma.
High-grade MALT lymphomas often contain low-grade tumour components; both cell populations have been shown to express the same immunoglobulin light chain previously. However, the clonal link between the low and high-grade components has not been established at the genetic level. To investigate this link, we have examined low- and high-grade components micro-dissected from tissue sections of four high-grade gastric MALT lymphomas. PCR and sequence analyses were performed to identify clone-specific rearranged immunoglobulin heavy chain gene sequences. In each of these cases, the PCR products from the two components were identical in size by electrophoresis. Direct sequencing revealed common clone-specific immunoglobulin heavy chain gene rearrangements in both lesions of each case, providing genetic evidence for a clonal link. These results support the proposal that high-grade MALT lymphomas generally evolve from low-grade clones.
Peng
H
H
Department of Histopathology, University College London Medical School, UK.
Du
M
M
Diss
T C
TC
Isaacson
P G
PG
Pan
L
L
D001483:Base Sequence; D019070:Cell Lineage; D002999:Clone Cells; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012689:Sequence Homology, Nucleic Acid; D013274:Stomach Neoplasms / Q000235:genetics*
D007143:Immunoglobulin Heavy Chains