Comparison between dual and tri-colour reagents for the analysis of lymphocyte subsets.
The aim of this project was to compare dual and tri-colour reagents for lymphocyte immunophenotyping. A total of 37 patient and normal specimens were immunophenotyped concurrently with the following mean values (% dual vs tri-colour): CD3 (69.4 vs 68.3) CD4 (24.0 vs 24.2) and CD19 (13.9 vs 12.6). A comparison of the results obtained using the paired t test showed that there were no significant differences for cells expressing CD3, CD4 and CD19. However, there was a significant difference in the NK (18.3 vs 16.3) cell component. A major advantage in using 3 colour immunophenotyping is the ability to analyse specimens that cannot be analysed using dual colour reagents due to debris or contamination of the gate with non-lymphocytic cells.
Dhaliwal
J S
JS
Division of Immunology, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
Quek
C K
CK
Balasubramaniam
T
T
Nasuruddin
B A
BA
D018941:Antigens, CD19 / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D004396:Coloring Agents; D005434:Flow Cytometry / Q000379:methods; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D007694:Killer Cells, Natural / Q000737:chemistry / Q000166:cytology; D016131:Lymphocyte Subsets / Q000737:chemistry / Q000166:cytology*
D018941:Antigens, CD19; D017252:CD3 Complex; D015704:CD4 Antigens; D004396:Coloring Agents; D006684:HLA-DR Antigens; D007202:Indicators and Reagents