Overexpression of Bcl-2 attenuates MPP+, but not 6-ODHA, induced cell death in a dopaminergic neuronal cell line.
In order to investigate the role of Bcl-2 in dopaminergic cells, we established a dopaminergic neuronal cell line (MN9D) stably expressing human Bcl-2 (MN9D/Bcl-2) or neomycin (MN9D/Neo). Overexpression of Bcl-2 in MN9D cells attenuated cell death due to treatment of mitochondrial electron transport inhibitors including N-methyl-4-phenylpyridinium, whereas it did not prevent cell death induced by reagents generating reactive oxygen species including 6-hydroxy-dopamine. Moreover, the rate of glucose uptake in MN9D/Bcl-2 was significantly lower than that in MN9D/Neo after MPP+ treatment. Thus, Bcl-2 may counter aberrations in mitochondrial electron transfer processes by altering energy metabolism within the MN9D cells.
Oh
Y J
YJ
Department of Biology, Yonsei University School of Natural Sciences, Seoul, Korea.
Wong
S C
SC
Moffat
M
M
O'Malley
K L
KL
D015655:1-Methyl-4-phenylpyridinium / Q000737:chemistry*; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D018929:Cell Culture Techniques; D016923:Cell Death; D002470:Cell Survival; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007120:Immunochemistry; D016627:Oxidopamine / Q000737:chemistry*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D020112:Rhodamine 123; D012235:Rhodamines / Q000378:metabolism
D000911:Antibodies, Monoclonal; D019253:Proto-Oncogene Proteins c-bcl-2; D012235:Rhodamines; D020112:Rhodamine 123; D016627:Oxidopamine; D005947:Glucose; D015655:1-Methyl-4-phenylpyridinium