Hair follicle dermal papilla cell lines from p53-knockout mice.
We have attempted to establish a cell line from hair follicle dermal papillae microdissected from vibrissa of mice lacking p53 tumor suppressor gene. The hair follicle dermal papillae were obtained from three types of mice: null knockout, hetero-knockout and wild type litters. Continuous cell lines with short doubling time were obtained only from null knockout litter-mates. Dermal papilla cells from either the hetero-knockout or wild type mice did not multiply well, and died within three passages; provided, however, that in only three cases out of 40 primary dermal papilla implants from two hetero-knockout mice, the cell culture reached 29 passages with a much slower growth rate than the cell lines from the null-knockout mice. These data are in accordance with the notion that the loss of p53 function is closely related to perpetuation of cell cycles and or immortalization.
Hanzawa
C
C
Shiseido Research Center, Yokohama, Japan.
Kobayashi
K
K
Hirabayashi
Y
Y
Inoue
T
T
Aizawa
S
S
Adachi
K
K
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D017690:Cell Transplantation; D002478:Cells, Cultured; D004210:Dissection; D016158:Genes, p53 / Q000235:genetics*; D018859:Hair Follicle / Q000166:cytology*; D006579:Heterozygote; D007668:Kidney; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D012867:Skin / Q000166:cytology*; D012879:Skin Physiological Phenomena; D016042:Transplantation, Heterotopic