title: The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor.
abstract: Vertebrate limb formation has been known to be initiated by a factor(s) secreted from the lateral plate mesoderm. In this report, we provide evidence that a member of the fibroblast growth factor (FGF) family, FGF10, emanates from the prospective limb mesoderm to serve as an endogenous initiator for limb bud formation. Fgf10 expression in the prospective limb mesenchyme precedes Fgf8 expression in the nascent apical ectoderm. Ectopic application of FGF10 to the chick embryonic flank can induce Fgf8 expression in the adjacent ectoderm, resulting in the formation of an additional complete limb. Expression of Fgf10 persists in the mesenchyme of the established limb bud and appears to interact with Fgf8 in the apical ectoderm and Sonic hedgehog in the zone of polarizing activity. These results suggest that FGF10 is a key mesenchymal factor involved in the initial budding as well as the continuous outgrowth of vertebrate limbs.
authors.affiliation: Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohuchi
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: A
authors.initials: A
authors.lastname: Yamamoto
authors.forename: A
authors.initials: A
authors.lastname: Araga
authors.forename: T
authors.initials: T
authors.lastname: Ohata
authors.forename: Y
authors.initials: Y
authors.lastname: Ishimaru
authors.forename: H
authors.initials: H
authors.lastname: Yoshioka
authors.forename: T
authors.initials: T
authors.lastname: Kuwana
authors.forename: T
authors.initials: T
authors.lastname: Nohno
authors.forename: M
authors.initials: M
authors.lastname: Yamasaki
authors.forename: N
authors.initials: N
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Noji
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D003001:Cloning, Molecular; D004475:Ectoderm / Q000378:metabolism; D051526:Fibroblast Growth Factor 10; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000502:physiology*; D005347:Fibroblasts / Q000637:transplantation; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances / Q000502:physiology*; D053823:Hedgehog Proteins; D018878:Limb Buds / Q000254:growth & development*; D008648:Mesoderm / Q000378:metabolism; D008969:Molecular Sequence Data; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D015534:Trans-Activators
chemical_list: D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D053823:Hedgehog Proteins; D011506:Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors