title: Immunoreactive p53 and metallothionein expression in duct carcinoma in situ of the breast. No correlation.
abstract: Immunocytochemically detectable MT and p53 have been found more commonly in comedo DCIS of the breast with high-grade cytology. The aim of this study is to confirm these findings and to investigate the relationship between MT and p53 in a single large series of cases of DCIS of the breast. To this end, 127 cases of DCIS were classified histologically according to architecture, cytonuclear differentiation (grade), presence and extent of intraduct necrosis, and using the Van Nuys system. Sections were immunostained for p53 and MT (E9) using established techniques, and the extent and intensity of staining were assessed semi-quantitively. The results confirmed that there was generally more MT and p53 positivity in poorly differentiated (grade 3) DCIS with extensive necrosis and that MT expression was greater in grade 2 lesions than p53 expression. However, overall there was no statistically significant correlation between p53 and MT staining. The results indicate that MT and p53 overexpression may arise from independent mechanisms in early breast neoplasia.
authors.affiliation: Department of Pathology, University of Wales College of Medicine, Cardiff, UK.
authors.forename: A G
authors.initials: AG
authors.lastname: Douglas-Jones
authors.forename: H
authors.initials: H
authors.lastname: Navabi
authors.forename: J M
authors.initials: JM
authors.lastname: Morgan
authors.forename: B
authors.initials: B
authors.lastname: Jasani
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D002278:Carcinoma in Situ / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008668:Metallothionein / Q000032:analysis* / Q000235:genetics; D009336:Necrosis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53; D008668:Metallothionein