title: High density lipoprotein decreases beta-amyloid toxicity in cortical cell culture.
abstract: A hallmark of Alzheimer's disease (AD) is the extracellular deposition and accumulation of a 39-43 amino peptide, known as the amyloid beta (A beta) protein, within the brain. It has been postulated that A beta may in some way contribute directly to AD pathogenesis. The epsilon 4 allele of apolipoprotein E (apoE) is a major AD risk factor. Since both apoE and A beta are components of lipoproteins in plasma and cerebrospinal fluid, we asked whether lipoproteins and apoE isoforms would modify the toxicity of A beta (1-42) in cortical cell cultures. We show that high density lipoprotein with or without apoE reduces A beta toxicity and that apoE in the absence of lipoproteins does not affect A beta toxicity. These results suggest that interactions between A beta and lipoproteins in the brain could influence AD pathogenesis.
authors.affiliation: Department of Neurology, Washington University School of Medicine, St Louis, MO 63110, USA.
authors.forename: Z S
authors.initials: ZS
authors.lastname: Farhangrazi
authors.forename: H
authors.initials: H
authors.lastname: Ying
authors.forename: G
authors.initials: G
authors.lastname: Bu
authors.forename: L L
authors.initials: LL
authors.lastname: Dugan
authors.forename: A M
authors.initials: AM
authors.lastname: Fagan
authors.forename: D W
authors.initials: DW
authors.lastname: Choi
authors.forename: D M
authors.initials: DM
authors.lastname: Holtzman
mesh_terms: D000483:Alleles; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000818:Animals; D001057:Apolipoproteins E / Q000032:analysis / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects*; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D012307:Risk Factors
chemical_list: D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D008075:Lipoproteins, HDL