title: Effects of dichloroisoproterenol on macromolecular synthesis and differentiation in Tetrahymena vorax.
abstract: The effect of dichloroisoproterenol on macrostomal cell formation in Tetrahymena vorax was examined and the drug was found to be 50% inhibitory at a concentration of 88 microM. Cellular uptake and incorporation of a variety of radiolabelled precursors was monitored in the presence of dichloroisoproterenol. The results demonstrate a strong, concentration-dependent inhibitory effect on RNA and protein biosynthesis, with a lesser inhibition observed for lipid biosynthesis. These data indicate that dichloroisoproterenol's reported effects on vacuole formation and processing in Tetrahymena are nonspecific with regard to phagocytic processes, but result from a general suppression of macromolecular synthesis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi 39762, USA. per@ra.msstate.edu
authors.forename: P E
authors.initials: PE
authors.lastname: Ryals
authors.forename: X
authors.initials: X
authors.lastname: Yang
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D007545:Isoproterenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis; D015800:Protozoan Proteins / Q000096:biosynthesis; D016053:RNA, Protozoan / Q000096:biosynthesis; D013768:Tetrahymena / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000319:Adrenergic beta-Antagonists; D008055:Lipids; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D007545:Isoproterenol; C084581:dichloroisoproterenol