Serological comparison of tospoviruses with polyclonal antibodies produced against the main structural proteins of tomato spotted wilt virus.
A new purification procedure for the tospoviruses of serogroups II and III was developed. SDS-polyacrylamide gel electrophoresis of purified tomato spotted wilt virus (TSWV; serogroup I), groundnut ringspot virus (GRSV: serogroup II), tomato chlorotic spot virus (TCSV; serogroup II) and impatiens necrotic spot virus (INSV; serogroup III) showed that the glycoprotein G2 of serogroup II members differs significantly in size from that of the serogroup I virus. Western immunoblot analysis using polyclonal antisera produced against purified glycoproteins TSWV-G1 and G2 as well as peptides of hydrophilic sequences of TSWV-G1 and G2 expressed in Escherichia coli demonstrated a higher homology amongst G1 of different serogroups than for G2. These results are supported by comparing the glycoprotein gene sequences of different serogroups.
Feldhoff
A
A
Department of Virology, Agricultural University of Wageningen, The Netherlands.
Kikkert
M
M
Kormelink
R
R
Krczal
G
G
Goldbach
R
R
Peters
D
D
D000914:Antibodies, Viral / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D017865:Tospovirus / Q000145:classification* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism
D000914:Antibodies, Viral; D007106:Immune Sera; D014759:Viral Envelope Proteins