Three-dimensional organization of a human water channel.
Aquaporins (AQP) are members of the major intrinsic protein (MIP) superfamily of integral membrane proteins and facilitate water transport in various eukaryotes and prokaryotes. The archetypal aquaporin AQP1 is a partly glycosylated water-selective channel that is widely expressed in the plasma membranes of several water-permeable epithelial and endothelial cells. Here we report the three-dimensional structure of deglycosylated, human erythrocyte AQP1, determined at 7 A resolution in the membrane plane by electron crystallography of frozen-hydrated two-dimensional crystals. The structure has an inplane, intramolecular 2-fold axis of symmetry located in the hydrophobic core of the bilayer. The AQP1 monomer is composed of six membrane-spanning, tilted alpha-helices. These helices form a barrel that encloses a vestibular region leading to the water-selective channel, which is outlined by densities attributed to the functionally important NPA boxes and their bridges to the surrounding helices. The intramolecular symmetry within the AQP1 molecule represents a new motif for the topology and design of membrane protein channels, and is a simple and elegant solution to the problem of bidirectional transport across the bilayer.
Cheng
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Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
van Hoek
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Yeager
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Verkman
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Mitra
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D051398:Aquaporin 1; D020346:Aquaporins; D001789:Blood Group Antigens; D003460:Crystallization; D005615:Freezing; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007473:Ion Channels / Q000737:chemistry* / Q000648:ultrastructure; D008958:Models, Molecular; D011487:Protein Conformation; D014867:Water
C496020:AQP1 protein, human; D020346:Aquaporins; D001789:Blood Group Antigens; D007473:Ion Channels; D014867:Water; D051398:Aquaporin 1