Isolation and characterization of a Listeria monocytogenes mutant strain hyperproducing virulence factors.
A mutant strain characterized by hyperproduction of a number of cell wall and supernatant proteins was isolated after N'methyl-N'-nitro-N-nitrosoguanidine treatment of Listeria monocytogenes strain NCTC10527. Several of these proteins were identified as virulence factors. When a wild-type strain was grown in the presence of activated charcoal it was shown to exhibit the same protein pattern as the isolated mutant.
Ermolaeva
S
S
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia. sveta@ermolaeva.msk.su
Varfolomeeva
N
N
Belyi
Y
Y
Tartakovskii
I
I
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001427:Bacterial Toxins; D002473:Cell Wall / Q000378:metabolism; D002606:Charcoal / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006460:Hemolysin Proteins; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000235:genetics; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010740:Phospholipases / Q000096:biosynthesis / Q000235:genetics; D010738:Type C Phospholipases / Q000096:biosynthesis / Q000235:genetics; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D008769:Methylnitronitrosoguanidine; D002606:Charcoal; D010740:Phospholipases; D010738:Type C Phospholipases; D008666:Metalloendopeptidases; D043265:Phosphatidylinositol Diacylglycerol-Lyase; C038705:hlyA protein, Listeria monocytogenes