title: Cloning and molecular characterization of plant aldehyde oxidase.
abstract: Primary structural information of a plant aldehyde oxidase (AO), which was purified from maize coleoptiles using indole-3-acetaldehyde as a substrate, was obtained by sequencing a series of cleavage peptides, permitting the cloning of the corresponding cDNA (zmAO-1). The complete nucleotide sequence was determined; the deduced amino acid sequence encodes a protein of 1358 amino acid residues of Mr 146,681, which is consistent with the size of the AO monomeric subunit. There is a significant similarity with AO from mammals and xanthine dehydrogenases from various sources. The maize AO polypeptide contains consensus sequences for iron-sulfur centers and a putative molybdopterin cofactor-binding domain. In addition, another cDNA (zmAO-2), highly homologous to zmAO-1 at both the nucleotide and amino acid sequence levels, was cloned. zmAO-2 would encode a protein of 1349 amino acid residues of Mr 145,173 and has molecular characteristics similar to those of zmAO-1. zmAO-1 was expressed at a high level in roots, which was closely correlated with immunoblotting results using antiserum raised against the purified maize AO protein, whereas zmAO-2 was expressed at a higher level in coleoptiles than in roots. We propose each zmAO may have a unique function during plant development.
authors.affiliation: Frontier Research Program, Wako-shi, Saitama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sekimoto
authors.forename: M
authors.initials: M
authors.lastname: Seo
authors.forename: N
authors.initials: N
authors.lastname: Dohmae
authors.forename: K
authors.initials: K
authors.lastname: Takio
authors.forename: Y
authors.initials: Y
authors.lastname: Kamiya
authors.forename: T
authors.initials: T
authors.lastname: Koshiba
mesh_terms: D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary; D006868:Hydrolysis; D007106:Immune Sera; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D007106:Immune Sera; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase