Chromosomal localization of the human and mouse hyaluronan synthase genes.
We have recently identified a new vertebrate gene family encoding putative hyaluronan (HA) synthases. Three highly conserved related genes have been identified, designated HAS1, HAS2, and HAS3 in humans and Has1, Has2, and Has3 in the mouse. All three genes encode predicted plasma membrane proteins with multiple transmembrane domains and approximately 25% amino acid sequence identity to the Streptococcus pyogenes HA synthase, HasA. Furthermore, expression of any one HAS gene in transfected mammalian cells leads to high levels of HA biosynthesis. We now report the chromosomal localization of the three HAS genes in human and in mouse. The genes localized to three different positions within both the human and the mouse genomes. HAS1 was localized to the human chromosome 19q13.3-q13.4 boundary and Has1 to mouse Chr 17.HAS2 was localized to human chromosome 8q24.12 and Has2 to mouse Chr 15. HAS3 was localized to human chromosome 16q22.1 and Has3 to mouse Chr 8. The map position for HAS1 reinforces the recently reported relationship between a small region of human chromosome 19q and proximal mouse chromosome 17. HAS2 mapped outside the predicted critical region delineated for the Langer-Giedion syndrome and can thus be excluded as a candidate gene for this genetic syndrome.
Spicer
A P
AP
Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Arizona 85259, USA. spicer@mayo.edu
Seldin
M F
MF
Olsen
A S
AS
Brown
N
N
Wells
D E
DE
Doggett
N A
NA
Itano
N
N
Kimata
K
K
Inazawa
J
J
McDonald
J A
JA
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D014453:Glucuronosyltransferase / Q000235:genetics*; D016695:Glycosyltransferases; D006801:Humans; D000076002:Hyaluronan Synthases; D008565:Membrane Proteins; D051379:Mice; D013045:Species Specificity; D014166:Transferases; D029867:Xenopus Proteins
D017931:DNA Primers; D008565:Membrane Proteins; D029867:Xenopus Proteins; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; C101754:HAS2 protein, human; C105458:HAS3 protein, human; C491096:Has2 protein, Xenopus; C491099:Has2 protein, mouse; C491356:Has3 protein, mouse; D000076002:Hyaluronan Synthases