Microsatellite polymorphism in the promoter sequence of the elongation factor 3 gene of Candida albicans as the basis for a typing system.
The polymorphism of a TTC/TTTC microsatellite in the promoter sequence of the elongation factor 3 gene of Candida albicans was investigated by PCR. One primer was fluorescein labeled, and PCR signals were read with an automatic sequencer. Twenty-nine reference strains and 31 independent clinical isolates were studied. Eleven different alleles were identified, giving 16 different profiles among the 60 strains tested, with a discriminatory power of 0.88. This marker is stable upon subculture, and reproducibility was achieved by automated procedures. When several microsatellite markers are available, many isolates can be rapidly and reproducibly tested for epidemiological questions, such as the prevalence of a given strain in a hospital setting and transmission between patients.
Bretagne
S
S
Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor-Assistance Publique, Créteil, France. bretagne@univ-paris12.fr
Costa
J M
JM
Besmond
C
C
Carsique
R
R
Calderone
R
R
D002176:Candida albicans / Q000145:classification* / Q000235:genetics; D005656:Fungal Proteins; D005800:Genes, Fungal; D016533:Mycological Typing Techniques; D010445:Peptide Elongation Factors / Q000235:genetics*; D011110:Polymorphism, Genetic; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; C111584:YEF3 protein, S cerevisiae