[Allergenicity and specific protein profiles of mange mites Chorioptes bovis, Psoroptes ovis (Acari: Psoroptidae), Saroptes suis and Notoedres cati (Acari: Sarcoptidae) using SDS-PAGE and immunoblotting].
A main point of immunoparasitological research in regard to pest arthropod-infestation is biochemical and immunological characterization of antigens. Precondition of own examinations to the specific protein pattern of mange mites were in quality and amount sufficient antigen preparations in mite extract solutions. For mite separation and antigen refinement field strains of Chorioptes bovis, Psoroptes ovis, Sarcoptes suis and Notoedres cati from definitive host animals cattle, sheep, pig and cat have been used. Parasites were isolated in a migration procedure. After having applicated subepidermally a low dose of mite extract solutions in sensitized animals allergic skin changes (Immediate reaction type 1) became apparent. SDS-PAGE exhibited specific protein patterns of 4 pathogen mite species. For Chorioptes bovis 16, Psoroptes ovis 15, Sarcoptes suis 27, and Notoedres cati 36 fractions have been detected. Proteins are antigens or allergen structures to be found in saliva, faecal output or moulting products of developmental stages and other metabolites of the parasites. Protein components were transferred onto nitrocellulosis. Immunoblotting made fractions with antigen activity visible.
Beck
W
W
Institut für Parasitologie und Tropenveterinärmedizin (Standort Mitte), Freien Universität Berlin.
Hiepe
T
T
D000485:Allergens; D000818:Animals; D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification; D002415:Cats; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006969:Hypersensitivity, Immediate; D007114:Immunization; D015151:Immunoblotting; D008925:Mites / Q000276:immunology*; D011506:Proteins / Q000276:immunology* / Q000302:isolation & purification; D012756:Sheep; D012882:Skin Tests; D013045:Species Specificity; D013552:Swine
D000485:Allergens; D000941:Antigens; D011506:Proteins