title: Effect of cadmium injury on growth and migration of cultured human vascular endothelial cells.
abstract: The effect of cadmium chloride (CdCl2) on the cell proliferation and migration of cultured human umbilical vein endothelial cells (HUVECs) was quantitatively analyzed. The HUVEC viable cell count decreased dose-dependently after exposure to Cd (cadmium chloride, 10 nM-1 mM). Morphologic examination by phase-contrast microscopy revealed severe damaging effects of Cd at higher concentrations. The cytotoxic effect of Cd (10 microM-1 mM) on DNA synthesis was also concentration-dependent. When the distance endothelial cells grew out from the scraped edge of a monolayer was measured, HUVEC outgrowth was found to be inhibited by Cd (1.0 microM-1.0 mM) in a dose-dependent manner. These findings suggest that HUVEC cell proliferation and migration are susceptible to Cd cytotoxicity, and that this may be involved in the pathogenesis of atherosclerosis.
authors.affiliation: Department of Environmental Medicine, Shimane Medical University.
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
authors.forename: T
authors.initials: T
authors.lastname: Oguri
authors.forename: S
authors.initials: S
authors.lastname: Yamabe
authors.forename: M
authors.initials: M
authors.lastname: Tada
mesh_terms: D019256:Cadmium Chloride / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects*; D006801:Humans
chemical_list: D004247:DNA; D019256:Cadmium Chloride