The dorsal tegmentum of the pontomesencephalic junction of the rat--immunohistochemistry (choline acetyltransferase, tyrosine hydroxylase, substance P) and NADPH-diaphorase histochemistry in frontal and horizontal sections.
37 complete frontal and horizontal series of rat brain were studied to compare the distribution of choline acetyltransferase- (ChAT), tyrosine hydroxylase- (TH), substance P- (SP), calbindin D- (Calb) and NADPH-diaphorase (NADPH-d)-positive cells within the cytoarchitectonic borders of the latero-dorsal tegmental nucleus (L-D) and its neighbourhood. We found the same distribution, number and morphology of NADPH-d-positive cells and ChAT-positive cells. Rostrally, there are no borders between NADPH-d-positive cells of L-D and NADPH-d-positive cells of the lateral part of the dorsal raphe nucleus. Only a few TH-positive cells are intermingled with ChAT/NADPH-d-positive cells at the lateral border of L-D. TH-positive cells are larger or the same size as cholinergic neutrons. Locus coeruleus and its rostral part is full of TH-positive cells and their fibres run ventromedially towards L-D. Barrington's nucleus appears in double staining (ChAT and TH or NADPH-d and TH) as an empty area bordered by ChAT- or NADPH-d-positive cells of L-D and TH-positive fibres of the locus coeruleus. Some of these fibres run through the Barrington's nucleus. The shape and size of SP-positive neurons is the same as ChAT- and NADPH-d-positive neurons. SP-positive neurons are sparsely distributed in all parts of L-D, but there are only a few SP-positive cells in its medial part. About 50% of the ChAT- and NADPH-d-positive cells are also SP-positive. Results are expressed by figures in three representative frontal sections and one horizontal section through the dorsal mesopontine tegmentum.
Nĕmcová
V
V
Anatomical Institute of the First Medical Faculty, Charles University, Prague, Czech Republic.
Petrovický
P
P
ten Donkelaar
H J
HJ
D000818:Animals; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000033:anatomy & histology / Q000166:cytology* / Q000201:enzymology; D009252:NADPH Dehydrogenase / Q000032:analysis*; D011149:Pons / Q000033:anatomy & histology / Q000166:cytology* / Q000201:enzymology; D011903:Raphe Nuclei / Q000033:anatomy & histology / Q000166:cytology / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000032:analysis*; D013681:Tegmentum Mesencephali / Q000033:anatomy & histology / Q000166:cytology* / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D013373:Substance P; D014446:Tyrosine 3-Monooxygenase; D009252:NADPH Dehydrogenase; D002795:Choline O-Acetyltransferase