Interleukin-17 up-regulation of nitric oxide production in human osteoarthritis cartilage.
OBJECTIVE
To examine the effect of human interleukin-17 (IL-17) on nitric oxide (NO) production in human osteoarthritis (OA) cartilage under ex vivo conditions.


METHODS
OA cartilage from patients undergoing knee replacement surgery was used in explant assays to assess the effect of IL-17. NO production was measured by estimating the stable NO metabolite, nitrite, in conditioned medium.


RESULTS
IL-17 augmented the spontaneous production of nitric oxide. This augmentation was sensitive to cycloheximide and pyrrolidine dithiocarbamate, but not to dexamethasone or soluble IL-1 receptor.


CONCLUSIONS
IL-17 augments nitric oxide production in OA cartilage via nuclear factor kappaB activation, but independently of IL-1beta signaling.
Attur
M G
MG
Hospital for Joint Diseases, New York, New York 10003, USA.
Patel
R N
RN
Abramson
S B
SB
Amin
A R
AR
D000368:Aged; D002358:Cartilage, Articular / Q000378:metabolism*; D016207:Cytokines / Q000502:physiology; D006801:Humans; D020381:Interleukin-17; D007378:Interleukins / Q000502:physiology*; D007719:Knee Joint; D007720:Knee Prosthesis; D008875:Middle Aged; D009569:Nitric Oxide / Q000096:biosynthesis*; D010003:Osteoarthritis / Q000378:metabolism*; D015854:Up-Regulation
D016207:Cytokines; D020381:Interleukin-17; D007378:Interleukins; D009569:Nitric Oxide