Alternatively spliced isoforms of the Na+/Ca2+ exchanger in the guinea pig cochlea.
The cochlea has been suggested to express some Na+/ Ca2+ exchangers (NCX), since efficient acoustic transduction requires cytosolic calcium homeostasis. The present study revealed that several spliced isoforms of NCX are expressed in the guinea pig cochlea. Moreover, to determine their localization in the cochlea, microdissected RT-PCR was performed. The guinea pig cochlea was microdissected into three parts (lateral wall, the organ of Corti and modiolus). The cochlear lateral wall and the organ of Corti expressed only a single isoform of NCX1. On the other hand, five isoforms of NCX1 and four isoforms of NCX3 were detected in the cochlear modiolus. The alternative splicing may provide diverse functions for NCX in the cochlea.
Oshima
T
T
Department of Otolaryngology, Tohoku University School of Medicine 1-1, Sendai, Japan.
Ikeda
K
K
Furukawa
M
M
Takasaka
T
T
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D003051:Cochlea / Q000033:anatomy & histology / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D009925:Organ of Corti / Q000033:anatomy & histology / Q000378:metabolism; D016133:Polymerase Chain Reaction; D019831:Sodium-Calcium Exchanger; D013136:Spiral Ganglion / Q000033:anatomy & histology / Q000378:metabolism; D014018:Tissue Distribution
D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D019831:Sodium-Calcium Exchanger