Aluminum fluoride associates with the small guanine nucleotide binding proteins.
AlF4- has long been known to associate with and activate the GDP-bound alpha subunits of heterotrimeric G-proteins. Recently the small guanine nucleotide binding protein Ras has also been shown to associate with AlF4- in the presence of stoichiometric amounts of its GTPase activating protein (GAP). Here we present the isolation of a stable Ras x GDP- x AlF4- x GAP ternary complex by gel filtration. In addition, we generalise the association of AlF4- with the small GTP-binding proteins by demonstrating ternary complex formation for the Cdc42, Rap and Ran proteins in the presence of their respective GAP proteins.
Ahmadian
M R
MR
Max-Planck-Institut für molekulare Physiologie, Dortmund, Germany.
Mittal
R
R
Hall
A
A
Wittinghofer
A
A
D017607:Aluminum Compounds / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000302:isolation & purification / Q000378:metabolism; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004926:Escherichia coli; D005459:Fluorides / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; D020931:ran GTP-Binding Protein; D020668:rap GTP-Binding Proteins; D020703:ras GTPase-Activating Proteins; D018631:ras Proteins / Q000302:isolation & purification / Q000378:metabolism*
D017607:Aluminum Compounds; D018797:Cell Cycle Proteins; D020690:GTPase-Activating Proteins; D009687:Nuclear Proteins; D011506:Proteins; D011994:Recombinant Proteins; D020703:ras GTPase-Activating Proteins; C050992:tetrafluoroaluminate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; D020931:ran GTP-Binding Protein; D020668:rap GTP-Binding Proteins; D018631:ras Proteins; D005459:Fluorides