Elution and reconcentration of coliphages in water from positively charged membrane filters with urea-arginine phosphate buffer.
Coliphages in drinking water and waste water samples have been adsorbed onto positively charged membrane filters, eluted with urea-arginine phosphate buffer (UAPB), reconcentrated, and detected with Escherichia coli C (ATCC 13706). The proposed membrane filter-based UAPB method for concentration and detection of coliphages compares favorably with the beef extract elution and reconcentration procedure and also with the proposed coliphage detection procedure described in Standard Methods for the Examination of Water and Wastewater. The higher recovery of coliphages with UAPB elution from positively charged membrane filters is attributed to testing the whole volume of concentrated sample, rather than partial analysis of the sample as in the procedure described in Standard Methods for the Examination of Water and Wastewater, especially when the titre is very low.
Jothikumar
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Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis 95616-8743, USA.
Cliver
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D001120:Arginine / Q000378:metabolism*; D002021:Buffers; D003090:Coliphages / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism / Q000821:virology; D007477:Ions; D008851:Micropore Filters; D010710:Phosphates / Q000378:metabolism*; D014508:Urea / Q000378:metabolism*; D014871:Water Microbiology
D002021:Buffers; D007477:Ions; D010710:Phosphates; D014508:Urea; D001120:Arginine