title: Leucocyte and platelet-derived bioactive substances in stored blood: effect of prestorage leucocyte filtration.
abstract: Adverse reactions to transfusion of allogeneic blood may depend on content of leucocytes and platelets and on storage-time of the erythrocyte suspensions. Therefore, we studied the efficacy of prestorage leucocyte reduction by filtration on total content and extracellular accumulation of histamine, eosinophil cationic protein (ECP), eosinophil protein X (EPX), myeloperoxidase (MPO), plasminogen activator inhibitor type-1 (PAI-1) and interleukin-6 (IL-6) in samples obtained from 5 units of SAGM blood, 7 units of plasma-reduced whole-blood and 6 units of whole-blood before and after filtration, respectively. In addition, we analysed supernatants from the same units after storage at +4 degrees C for 0, 21 and 35 d, respectively. The filtration was performed at room temperature within 2-4 h after donation. The substances were analysed by ELISA and RIA methods and we also analysed the donor plasma levels of the same bioactive substances. The total content of histamine, ECP, EPX, and MPO were 10-70-fold higher in all unfiltered erythrocyte products compared to donor plasma concentrations, while PAI-1 content was 15-20-fold higher only in plasma-reduced whole-blood and whole-blood. Prestorage leucocyte filtration significantly reduced the total histamine, ECP, EPX, MPO and PAI-1 content to levels similar to donor plasma levels in plasma-reduced whole-blood and whole-blood, while PAI-1 was still low in filtered SAGM blood. In addition, the levels of extracellular bioactive substances at d 0 after donation and filtration were within the range of concentrations in donor plasma, and there was no time-dependent accumulation during storage for 35 d at +4 degrees C. IL-6 was not detected in either plasma or samples obtained from the blood bags. These results suggest prestorage leucocyte filtration to deplete leucocyte contents to levels, which prevent the previously shown time-dependent accumulation of leucocyte derived bioactive substances in various erythrocyte suspensions. In addition, the PAI-1 results suggest leucocyte filters to reduce the obligatory platelet content in whole-blood products.
authors.affiliation: Surgical Immunology Laboratory, Hvidovre University Hospital, Denmark.
authors.forename: H J
authors.initials: HJ
authors.lastname: Nielsen
authors.forename: F
authors.initials: F
authors.lastname: Skov
authors.forename: E
authors.initials: E
authors.lastname: Dybkjaer
authors.forename: C M
authors.initials: CM
authors.lastname: Reimert
authors.forename: A N
authors.initials: AN
authors.lastname: Pedersen
authors.forename: N
authors.initials: N
authors.lastname: Brünner
authors.forename: P S
authors.initials: PS
authors.lastname: Skov
mesh_terms: D001685:Biological Factors / Q000097:blood*; D001782:Blood Donors; D001792:Blood Platelets / Q000166:cytology; D001798:Blood Proteins / Q000032:analysis; D001800:Blood Specimen Collection; D004797:Enzyme-Linked Immunosorbent Assay; D047091:Eosinophil Granule Proteins; D047208:Eosinophil-Derived Neurotoxin; D004804:Eosinophils; D005374:Filtration; D006632:Histamine / Q000097:blood; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D017708:Leukocyte Transfusion / Q000009:adverse effects; D007962:Leukocytes / Q000166:cytology; D009195:Peroxidase / Q000097:blood; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D017713:Platelet Transfusion / Q000009:adverse effects; D011863:Radioimmunoassay; D012260:Ribonucleases
chemical_list: D001685:Biological Factors; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D015850:Interleukin-6; D017395:Plasminogen Activator Inhibitor 1; D006632:Histamine; D009195:Peroxidase; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases