[The first member of a novel family of eukaryotic transcription factors detected by the heterospecific complementation].
The cDNA of a previously uncharacterized gene fet5 (factor of eukaryotic transcription, clone no. 5) of the fission yeast Schizosaccharomyces pombe was cloned by the heterospecific complementation of a conditional mutant of Saccharomyces cerevisiae defective in the function of the RNA polymerases I-III common subunit ABC10 beta. The gene encodes a new factor of eukaryotic transcription, Fet5, the first member of a superfamily of proteins for which the area of functioning is determined. The Fet5-superfamily consists of three distinct families of proteins highly evolutionarily conserved and widely spread among eukaryotes. Features of the Fet5 amino acid sequence suggest that it belongs to ATP/GTP-binding proteins.
Shpakovskiĭ
G V
GV
Lebedenko
E N
EN
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification
D018076:DNA, Complementary; D014157:Transcription Factors