Polyphenol oxidase activity staining in polyacrylamide electrophoresis gels.
An analytical method allowing the detection of polyphenol oxidase activity on polyacrylamide gel electrophoresis (PAGE) is described. The method is rapid, sensitive and specific and is based on a coupling reaction between 4-tert-butyl-o-benzoquinone and the aromatic amine, 4-amino-N,N-diethylaniline sulphate. Catecholase activity of polyphenol oxidase appears as blue stained bands on a colourless background.
Rescigno
A
A
Istituto di Chonica Biologica, Università di Cagliari, Italy. rescigno@vaxcal.unica.it
Sollai
F
F
Rinaldi
A C
AC
Soddu
G
G
Sanjust
E
E
D000818:Animals; D004156:Catechol Oxidase / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
D004156:Catechol Oxidase