title: Female rats do not develop sucrose-induced insulin resistance.
abstract: In male rats, 2 wk of high-sucrose feeding results in insulin resistance and hypertriglyceridemia [Pagliassotti, M.J., P.A. Prach, T.A. Koppenhafer, and D.A. Pan. Am. J. Physiol. 271 (Regulatory Integrative Comp. Physiol. 40): R1319-R1326, 1996]. The present study aimed to determine if female rats also become insulin resistant and hypertriglyceridemic in response to high-sucrose feeding. Female Wistar rats (7 wk old) were fed either a high-sucrose diet (68% energy) (SU) or a high-starch diet (68% energy) (ST) for 3, 5, or 8 wk. In each animal, glucose kinetics were measured using [3-(3)H]glucose under basal and hyperinsulinemic conditions (insulin infusion 4.0 mU.kg-1.min-1). Body weight and basal glucose kinetics were not different between diet groups at 3, 5, or 8 wk. Glucose infusion rate (mg.kg-1.min-1) was not different between groups (3 wk: 17.7 +/- 1.6 ST, 16.6 +/- 0.9 SU; 5 wk: 16.1 +/- 0.9 ST, 15.1 +/- 2.0 SU; 8 wk: 18.3 +/- 1.9 ST, 16.1 +/- 1.5 SU). Clamp rate of glucose appearance (mg.kg-1.min-1) was also not different between diet groups (3 wk: 4.0 +/- 1.6 ST, 3.6 +/- 1.4 SU; 5 wk: 2.6 +/- 1.0 ST, 2.3 +/- 1.14 SU; 8 wk: 5.9 +/- 1.8 ST, 7.7 +/- 1.2 SU). No difference was observed in plasma and tissue triglycerides or tissue glycogen between sucrose- and starch-fed animals. We therefore conclude that female rats, in contrast to males, do not develop sucrose-induced insulin resistance and hypertriglyceridemia.
authors.affiliation: Section of Pediatric Nutrition, University of Colorado Health Sciences Center, Denver 80262, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Horton
authors.forename: E C
authors.initials: EC
authors.lastname: Gayles
authors.forename: P A
authors.initials: PA
authors.lastname: Prach
authors.forename: T A
authors.initials: TA
authors.lastname: Koppenhafer
authors.forename: M J
authors.initials: MJ
authors.lastname: Pagliassotti
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D019422:Dietary Sucrose / Q000378:metabolism*; D002149:Energy Intake; D005260:Female; D006003:Glycogen / Q000378:metabolism; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D019422:Dietary Sucrose; D007328:Insulin; D014280:Triglycerides; D006003:Glycogen