title: Electrospray mass spectrometry of alpha and beta chains of selected hemoglobins and their TNBA and TNB conjugates.
abstract: The molecular weights of alpha and beta hemoglobin chains from 15 different vertebrate animal sources and 2 common human variants were determined by electrospray mass spectrometry and compared to the calculated masses based on published amino acid sequences. Conjugates were prepared for 14 of the globins using 2 traditional colorimetric derivatizing reagents, 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and trinitrobenzenesulfonic acid (TNBS), and the mass of each conjugate was determined by mass spectrometry.
authors.affiliation: Department of Chemistry (D9NM), Abbott Laboratories, Abbott Park, Illinois 60064. USA. adamczykm@apmac.abbott.com
authors.forename: M
authors.initials: M
authors.lastname: Adamczyk
authors.forename: J C
authors.initials: JC
authors.lastname: Gebler
mesh_terms: D000818:Animals; D002415:Cats; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D008970:Molecular Weight; D009579:Nitrobenzoates / Q000737:chemistry*; D010215:Papio; D013438:Sulfhydryl Compounds; D014301:Trinitrobenzenes / Q000737:chemistry*
chemical_list: D006454:Hemoglobins; D009579:Nitrobenzoates; D013438:Sulfhydryl Compounds; D014301:Trinitrobenzenes; C011136:thionitrobenzoic acid