title: Implantation of cultured human leptomeningeal cells into rat brain.
abstract: Since previous studies have shown that cells cultured from human leptomeninges can express neuronal and glial antigens under appropriate culture conditions [DeGiorgio L. A. et al. (1994) J. Neurol. Sci. 124, 141 148; Bernstein J. J. et al. (1996) Int. J. Derl Neurosci. 14(5), 681 687], we have studied the developmental characteristics of these cells further by grafting them into young adult rat brains. Cells were labeled in culture with Fast Blue and were identified unequivocally by hybridization with nick-translated human DNA. Intensely Fast Blue positive human leptomeningeal cells were concentrated in the implant pocket and adjacent rat leptomeninges al one and two weeks postimplant. Human and rat leptomeningeal cells were similar morphologically and were equally immunopositive for vimentin and fibronectin. Implanted human cells did not express the neuronal and glial proteins they had in vitro. Cells which hybridized with human DNA corresponded to the intensely Fast Blue positive cells. Small groups of human DNA hybridizing cells were also observed in the choroid plexus. Less intensely Fast Blue positive neurons and glia were found in the brain but these hybridized with rat DNA. A minority of human leptomeningeal cells implanted into rat brain are subsequently found in host leptomeninges where they demonstrate properties characteristic of leptomeningeal fibroblasts. Small numbers of implanted cells can survive for two weeks.
authors.affiliation: Altshul Laboratory for Dementia Research, Cornell University Medical College, Burke Medical Research Institute, White Plains, NY 10605, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: DeGiorgio
authors.forename: J J
authors.initials: JJ
authors.lastname: Bernstein
authors.forename: J P
authors.initials: JP
authors.lastname: Blass
mesh_terms: D000328:Adult; D000368:Aged; D000578:Amidines; D000818:Animals; D001099:Arachnoid / Q000166:cytology* / Q000502:physiology; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000601:surgery*; D002470:Cell Survival; D017690:Cell Transplantation; D004247:DNA / Q000235:genetics; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010841:Pia Mater / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous
chemical_list: D000578:Amidines; D005904:Glial Fibrillary Acidic Protein; C031455:diamidino compound 253-50; D004247:DNA