title: Clinical significance of human sperm-zona pellucida binding.
abstract: OBJECTIVE
  To assess the relationship between sperm morphology and motion parameters and sperm-zona pellucida (ZP) binding capacity under hemizona assay (HZA) conditions and to determine the discriminatory power of the HZA for the prediction of in vitro sperm fertilizing ability.
  
  
  METHODS
  Prospectively designed study.
  
  
  METHODS
  Academic tertiary centers.
  
  
  METHODS
  One hundred ninety-six couples undergoing IVF therapy participated in this study.
  
  
  METHODS
  Hemizona assay and IVF results were determined for each couple.
  
  
  METHODS
  Computerized sperm motion analysis, sperm morphology (strict) criteria), and HZA results were correlated with fertilization outcome.
  
  
  RESULTS
  Among sperm parameters from the original ejaculates, morphology was the best predictor of sperm-ZP binding ability; hyperactivated motility was the best predictor of HZA results after swim-up separation of the motile sperm fractions. The HZA index provided the highest discriminatory power for fertilization success/failure, with an overall accuracy of 86%.
  
  
  CONCLUSIONS
  Sperm morphology and hyperactivated motility showed a high correlation with the capacity of sperm to achieve tight binding to the ZP. The excellent positive and negative predictive values of the HZA for fertilization outcome provide additional support for the use of this functional bioassay in the decision-making process within the assisted reproduction setting.
authors.affiliation: Jones Institute for Women's Health, Department of Obstetrics and Gynecology, Norfolk, Virginia 23507, USA. SERGIO@JONES1.EVMS.EDU
authors.forename: S
authors.initials: S
authors.lastname: Oehninger
authors.forename: M
authors.initials: M
authors.lastname: Mahony
authors.forename: K
authors.initials: K
authors.lastname: Ozgür
authors.forename: P
authors.initials: P
authors.lastname: Kolm
authors.forename: T
authors.initials: T
authors.lastname: Kruger
authors.forename: D
authors.initials: D
authors.lastname: Franken
mesh_terms: D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D011446:Prospective Studies; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D016896:Treatment Outcome; D015044:Zona Pellucida / Q000502:physiology*