Surface plasmon resonance analysis of topoisomerase I-DNA binding: effect of Mg2+ and DNA sequence.
Surface plasmon resonance detection was used to characterize interactions of human topoisomerase I and DNA. The disassociation of topoisomerase I and DNA is characterized by two rate constants. This suggests two parallel but independent pathways of release. DNA association appears to be complex. Mg2+ was found to increase both disassociation rate constants and may have a detectable effect on topoisomerase I-DNA association. DNA base content did not affect disassociation rate constants or the rate of association.
Pond
C D
CD
Department of Pharmacology, University of Utah, Salt Lake City 84112, USA.
Holden
J A
JA
Schnabel
P C
PC
Barrows
L R
LR
D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques; D002384:Catalysis; D004247:DNA / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D012474:Salmon; D013057:Spectrum Analysis / Q000379:methods; D013379:Substrate Specificity
D004269:DNA, Bacterial; D004247:DNA; D004264:DNA Topoisomerases, Type I; D008274:Magnesium