Cloning and sequencing of the gene encoding the high potential iron-sulfur protein (HiPIP) from the purple sulfur bacterium Chromatium vinosum.
The gene encoding the high potential iron-sulfur protein (HiPIP) of Chromatium vinosum strain D (DSM 180T) was cloned from an EcoRI-HindIII digest of genomic DNA. A nucleotide sequence of 648 bp length was determined which contained the coding region and putative promoter and termination sites. The gene codes for a 122 residue 12761 Da protein. The C-terminal 85 residues are those of the previously biochemically determined sequence, whereas the N-terminal 37 residues constitute a leader peptide which shows characteristics of the double arginine signal sequences of complex cofactor containing periplasmic proteins.
Brüser
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Institut für Mikrobiologie and Biotechnologie, Bonn, Germany.
Trüper
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HG
Dahl
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002844:Chromatium / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D005798:Genes, Bacterial; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins; D021382:Protein Sorting Signals / Q000235:genetics
D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D021382:Protein Sorting Signals; C007943:high potential iron-sulfur protein; D004247:DNA