Analysis of extracellular matrix proteins in malignant chicken cell lines.
The adherent chicken cell line, MDCC-MSB1-41C, was highly transplantable and metastatic in vivo, compared with the parental non-adherent cell line MDCC-MSB1 from Marek's disease (MD) lymphoblastoid tumor. For clarification of differences in extracellular matrix proteins in MSB1-41C and MSB1 cells, examination was made of various components of extracellular matrix proteins. A detachment experiment indicated the protein(s) recognizing the Arg-Gly-Asp (RGD) sequence, the minimum structure required for recognition by the cell-surface receptors, is essential for the adherent character. Immunoblot assay using antibodies showed increased expression of fibronectin, fibronectin receptors, and vinculin on MSB1-41C cell lines. RGD-directed integrins mediate important cell-cell adhesive interaction and these interactions with extracellular matrix proteins may thus possibly be requisite for migration, proliferation and metastatic dissemination of MDCC-MSB1-41C cells. The RGD-containing peptide in the culture medium could cause detachment of cultured adhesive lymphoid leukosis LSCC-1104X5 cells from the dish too.
Koyama
T
T
National Institute of Animal Health, Ibaraki, Japan.
Nakajima
Y
Y
Miura
K
K
Yamazaki
M
M
Shinozaki
M
M
Kumagai
T
T
Sakaniwa
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002645:Chickens; D016326:Extracellular Matrix Proteins / Q000032:analysis* / Q000096:biosynthesis; D005353:Fibronectins / Q000032:analysis / Q000096:biosynthesis; D019084:Fluorescent Antibody Technique, Indirect; D015151:Immunoblotting; D016023:Integrins / Q000502:physiology; D008380:Marek Disease; D009842:Oligopeptides; D017469:Receptors, Fibronectin / Q000032:analysis / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D016596:Vinculin / Q000032:analysis / Q000096:biosynthesis
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D016023:Integrins; D009842:Oligopeptides; D017469:Receptors, Fibronectin; D016596:Vinculin; C047981:arginyl-glycyl-aspartic acid