title: Effect of dietary versus pharmacological correction of hypertriglyceridemia on red blood cell membrane sodium/lithium countertransport activity.
abstract: An elevated red blood cell Na/Li countertransport (Na/Li CT) is often associated with high blood pressure and metabolic abnormalities. Recent studies suggested that a reduction in serum TG levels is associated with a decrease in Na/Li CT activity. However, it is still unclear if this phenomenon could be originated from systemic metabolic alterations or from modifications of the membrane dynamic properties. Aim of the present study was to investigate whether dietary or pharmacological TG lowering therapy might have a different effect on Na/Li CT activity and related metabolic parameters. Twenty normotensive hyper-TG patients were recruited from the Lipid outpatient Clinic: they had a baseline Na/Li CT activity significantly higher compared with age- and BMI-matched normolipidemic controls (386+/-33 vs 274+/-39 umol/l RBC/h, p<0.05). The patients were randomly prescribed one of the following two-months treatment: Group 1)-triglyceride lowering diet; Group 2)-lipid lowering drug (Gemfibrozil 600 mg b.i.d.). Na/Li CT and metabolic and anthropometric variables were measured at baseline and after 1 and 2 months of treatment. At the end of intervention, there was in both groups a significant and comparable fall in plasma triglyceride (group 1: -2.61+/-0.73 mmol/l p<0.01; group 2: -4.29+/-1.20 mmol/l p<0.01). In the diet-treated group there were, in addition small but significant reductions in body weight (-3.7+/-0.8 kg p<0.01), fasting glucose (-0.36+/-0.14 mmol/l p<0.05) and insulin levels (-2.1+/-0.5 mU/l, p<0.01), while no such changes were observed in the fibrate treated patients. Na/Li CT activity was significantly and comparably reduced at the end of treatment in both groups (group 1: -97+/-28 umol/l cell/h, p<0.01; group 2: -89+/-30 umol/l cell/h, p<0.01). In conclusion, these results indicate that the decrease in Na/Li CT associated with both dietary and drug treatment of hypertriglyceridemia is to be traced to a direct effect of plasma TG concentration on this transport system (probably as a result of modification in the membrane lipid environment) rather than to changes in plasma insulin levels or insulin resistance.
authors.affiliation: Department of Clinical and Experimental Medicine, Federico II University of Naples Medical School, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Pagano
authors.forename: A
authors.initials: A
authors.lastname: Siani
authors.forename: P
authors.initials: P
authors.lastname: Pauciullo
authors.forename: C
authors.initials: C
authors.lastname: Lirato
authors.forename: R
authors.initials: R
authors.lastname: Iacone
authors.forename: A
authors.initials: A
authors.lastname: Sacchi
authors.forename: P
authors.initials: P
authors.lastname: Strazzullo
mesh_terms: D000328:Adult; D001692:Biological Transport; D002462:Cell Membrane / Q000187:drug effects; D004035:Diet Therapy; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D015248:Gemfibrozil / Q000494:pharmacology*; D006801:Humans; D015228:Hypertriglyceridemia / Q000188:drug therapy*; D008094:Lithium / Q000378:metabolism*; D008297:Male; D012964:Sodium / Q000378:metabolism*
chemical_list: D008094:Lithium; D012964:Sodium; D015248:Gemfibrozil