Expression of envelope protein (E2) of bovine viral diarrhea virus in insect cells.
The gene encoding the envelope glycoprotein (E2) of bovine viral diarrhea virus (BVDV) was expressed in a baculovirus. The expressed protein was detected on the surface of infected cells by immunofluorescence. Western blotting analysis showed the presence of the expressed protein of a similar molecular size to the E2 protein. The antigenicity of expressed protein were tested in guinea pigs and cattle. The immunized animals developed neutralizing antibodies against BVDV.
Kweon
C H
CH
National Veterinary Research Institute, Rural Development Administration, Anyang, Republic of Korea.
Yoon
Y D
YD
An
S H
SH
Lee
Y S
YS
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016367:Baculoviridae; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology; D002417:Cattle; D002460:Cell Line; D005822:Genetic Vectors; D006168:Guinea Pigs; D010576:Pestivirus / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D018411:Spodoptera; D014162:Transfection; D014759:Viral Envelope Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C076551:gp53, bovine viral diarrhea virus