Determination of N(G),N(G)-dimethylarginine in human plasma by high-performance liquid chromatography.
N(G),N(G)-Dimethylarginine (asymmetric dimethylarginine, ADMA) can be directly separated and measured from deproteinized human plasma using o-phthaldialdehyde-mercaptoethanol (OPA reagent) as a fluorogenic reagent by reversed-phase high-performance liquid chromatography. The mean recovery of ADMA was over 96% and the inter- and intra-assay coefficients of variation of amounts were lower than 3.80% and those of retention time were below 0.37% for five runs. The detection limit of the assay is 1 pmol when the signal-to-noise ratio is 3:1. It was observed that the concentration of ADMA was significantly elevated in plasma of patients with pregnancy induced hypertension (PIH) in contrast to healthy pregnant women.
Chen
B M
BM
Analytical Testing Center, Hunan Medical University, Changsha, China.
Xia
L W
LW
Zhao
R Q
RQ
D000596:Amino Acids / Q000097:blood / Q000302:isolation & purification; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000097:blood; D015203:Reproducibility of Results
D000596:Amino Acids; C018524:N,N-dimethylarginine; D001120:Arginine