Non-viral ex vivo hepatic gene transfer by in situ lipofection of liver and intraperitoneal transplantation of hepatocytes.
Perfusion of liver with plasmid DNA-lipofectin complexes via the portal vein results in efficient accumulation of the vector in hepatocytes. Such hepatocytes, when administered intraperitoneally into a hepatectomized rat, repopulate the liver and express the transgene efficiently. This procedure obviates the need for large-scale hepatocyte culture for ex vivo gene transfer. Further, intraperitoneal transplantation is a simple and cost-effective strategy of introducing genetically modified hepatocytes into liver. Thus, in situ lipofection of liver and intraperitoneal transfer of hepatocytes can be developed into a novel method of non-viral ex vivo gene transfer technique that has applications in the treatment of metabolic disorders of liver and hepatic gene therapy.
Rangarajan
P N
PN
Department of Biochemistry, Indian Institute of Science, Bangalore. ranga@biochem.iisc.ernet.in
Vatsala
P G
PG
Ashok
M S
MS
Srinivas
V K
VK
Habibullah
C M
CM
Padmanaban
G
G
D000818:Animals; D017690:Cell Transplantation; D005260:Female; D015870:Gene Expression; D006498:Hepatectomy; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008115:Liver Regeneration; D051379:Mice; D010537:Peritoneum; D011247:Pregnancy; D051381:Rats; D014162:Transfection / Q000379:methods*; D019076:Transgenes