title: Laminin-6 and laminin-5 are recognized by autoantibodies in a subset of cicatricial pemphigoid.
abstract: We characterized basement membrane zone (BMZ) autoantigens targeted by autoantibodies (AAb) from patients with cicatricial pemphigoid. Serum from a patient with severe oral cicatricial pemphigoid contained IgG anti-BMZ AAb. The AAb labeled a lower BMZ component on salt-split skin and localized to the lower lamina lucida/lamina densa by direct and indirect immunoelectron microscopy (IEM) but did not label blood vessels. The AAb did not react with EHS laminin-1 and type IV collagen, pepsinized human type IV collagen, recombinant entactin, or NC1 domain of type VII collagen by dot blotting and western blotting. We focused our studies on the laminin family, as laminin-5 was identified as an autoantigen in cicatricial pemphigoid. Culture-conditioned media from normal keratinocytes (containing laminin-6 and laminin-5) and JEB keratinocytes (containing laminin-6 but not laminin-5) were studied by western blotting. Under nonreducing conditions, the patient's AAb recognized a 600-kDa protein (laminin-6) intensely and a 400-kDa protein (laminin-5) weakly in normal keratinocyte medium even though abundant laminin-5 was present. InJEB keratinocyte medium, however, the 600-kDa protein (laminin-6) alone was recognized by the patient's AAb. The AAb also immunolabeled BMZ of JEB skin that lacked laminin-5. The AAb from this patient and two other patients with anti-laminin-5 cicatricial pemphigoid immunoprecipitated both laminin-6 and laminin-5. Taken together, the results of IEM, non-reducing western blotting, immunoprecipitation, and JEB skin BMZ immunolabeling indicate that laminin-6, as well as laminin-5, is identified by the AAb from a subset of cicatricial pemphigoid patients. We propose the name "anti-laminin cicatricial pemphigoid" for this subset.
authors.affiliation: Section of Dermatology, VA Lakeside Medical Center, Chicago, Illinois, Northwestern University Medical School, USA.
authors.forename: L S
authors.initials: LS
authors.lastname: Chan
authors.forename: A A
authors.initials: AA
authors.lastname: Majmudar
authors.forename: H H
authors.initials: HH
authors.lastname: Tran
authors.forename: F
authors.initials: F
authors.lastname: Meier
authors.forename: G
authors.initials: G
authors.lastname: Schaumburg-Lever
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Anhalt
authors.forename: D T
authors.initials: DT
authors.lastname: Woodley
authors.forename: M P
authors.initials: MP
authors.lastname: Marinkovich
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000032:analysis / Q000276:immunology*; D001485:Basement Membrane / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis / Q000276:immunology; D016109:Epidermolysis Bullosa, Junctional / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D007797:Laminin / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D010390:Pemphigoid, Benign Mucous Membrane / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D011233:Precipitin Tests; D012867:Skin / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D000097794:Kalinin
chemical_list: D001323:Autoantibodies; D015815:Cell Adhesion Molecules; D003094:Collagen; D007797:Laminin; D000097794:Kalinin