title: An in vivo dose-response study of fenbendazole against Oesophagostomum dentatum and Oesophagostomum quadrispinulatum in pigs.
abstract: A dose-response study using fenbendazole (FBZ) was carried out in pigs infected with O. dentatum and O. quadrispinulatum to determine the minimum effective dose rate of the drug. Thirty pigs were randomly divided into 6 groups of 5 pigs and infected with 5000 infective larvae each. The animals were re-infected 5 days before treatment (Day 30 after the first infection) with the same number of larvae. On Day 35 the pigs in groups 1-5 were treated with FBZ at the following dose rates: 2.5 mg kg-1 (i.e. 50% of the registered dose level), 1.0 mg kg-1 (20%), 0.25 mg kg-1 (5%), 0.1 mg kg-1 (2%) and 0.05 mg kg-1 (1%), respectively. Pigs in group 6 served as non-treated controls. Seven days after treatment (Day 42 after infection) the pigs were slaughtered, worms recovered from the large intestine and counted. The species and sex of adult worms was determined. A high faecal egg count reduction (FECR) after treatment was observed in groups 1, 2 and 3 (98%, 88% and 91%, respectively), while in groups 4 and 5 the egg counts were not affected by treatment. The mean worm count reduction was high in groups 1, 2 and 3 (100%, 99.9% and 98.6%, respectively), but declined in groups 4 and 5 (77% and 40%, respectively). FBZ showed a high efficacy against immature worms in groups 1 and 2, while in groups 3, 4 and 5 counts were not reduced. Species differentiation revealed a higher effect of FBZ against O. dentatum than against O. quadrispinulatum. Sex differentiation indicated a slightly higher (not significant) efficacy against females than males in both species. This study demonstrated a high efficacy of FBZ against the nodular worms in pigs, even at 5% of the currently registered dose level.
authors.affiliation: Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark. Jan.Praslicka@vetmi.kvl.dk
authors.forename: J
authors.initials: J
authors.lastname: Praslicka
authors.forename: H
authors.initials: H
authors.lastname: Bjørn
authors.forename: M
authors.initials: M
authors.lastname: Várady
authors.forename: P
authors.initials: P
authors.lastname: Nansen
authors.forename: D R
authors.initials: DR
authors.lastname: Hennessy
authors.forename: H
authors.initials: H
authors.lastname: Talvik
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005273:Fenbendazole / Q000627:therapeutic use*; D008297:Male; D009814:Oesophagostomiasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D010270:Parasite Egg Count; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000469:parasitology
chemical_list: D000969:Antinematodal Agents; D005273:Fenbendazole