title: Immobilization of streptavidin for immunosensors on nanostructured surfaces.
abstract: A modular immunosensor device based on silicon technology with electrochemical detection is presented. The sensor surface is functionalized with the antigen estradiol using the streptavidin-biotin system. The immobilized antigen is recognized by ferrocene-labeled antibody molecules in a competitive assay format. The ferrocene redox centers can be detected electrochemically. In order to facilitate the electron transfer, a gold layer structured in nanometer dimensions is used as an electrode. The functionalized silicon chip will be integrated into a micromachined fluid transport system. The resulting immunosensor device for estradiol represents a prototype allowing easy adaptation to other biotinylated analytes since the immobilized streptavidin serves as a universal anchor.
authors.affiliation: Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Kossek
authors.forename: C
authors.initials: C
authors.lastname: Padeste
authors.forename: L
authors.initials: L
authors.lastname: Tiefenauer
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry; D004958:Estradiol / Q000032:analysis; D012825:Silicon / Q000737:chemistry*; D019809:Streptavidin
chemical_list: D001426:Bacterial Proteins; D004958:Estradiol; D001710:Biotin; D019809:Streptavidin; D012825:Silicon