title: Isolation and possible composition of glucosylceramides from Paracoccidioides brasiliensis.
abstract: Twelve different species of neutral monohexosyl ceramides (CMHs) and two species of neutral monohexosyl ceramides were isolated from mycelium and yeast forms of Paracoccidioides brasiliensis, respectively, by a combination of ion-exchange chromatography, HPLC, and HPTLC. The glucosylceramides did not react with sera from patients with paracoccidioidomycosis (PCM). Carbohydrate analysis indicated that CMHs contain glucose. Analysis of 1H-NMR and mass spectrometry data suggest that the structure of the CMHs is Glcp beta 1-->Cer (mycelium forms present 12 different ceramides and yeast forms present 2 different ceramides). The composition of the lipid moieties was analyzed by negative fast atom bombardment mass spectrometry. No glycosphingolipid other than glucosylceramide was detected in P. brasiliensis.
authors.affiliation: Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brasil.
authors.forename: H K
authors.initials: HK
authors.lastname: Takahashi
authors.forename: S B
authors.initials: SB
authors.lastname: Levery
authors.forename: M S
authors.initials: MS
authors.lastname: Toledo
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: M E
authors.initials: ME
authors.lastname: Salyan
authors.forename: S
authors.initials: S
authors.lastname: Hakomori
authors.forename: A H
authors.initials: AH
authors.lastname: Straus
mesh_terms: D000818:Animals; D005963:Glucosylceramides / Q000302:isolation & purification*; D010228:Paracoccidioides / Q000737:chemistry*
chemical_list: D005963:Glucosylceramides