Molecular cloning of the interleukin-1 gene cluster: construction of an integrated YAC/PAC contig and a partial transcriptional map in the region of chromosome 2q13.
Genes of the interleukin-1 (IL-1) gene cluster localized on chromosome 2q13 are implicated in many physiological and pathophysiological processes. We present here a high-resolution physical map of this region between markers D2S2008 and D2S4/PAX8. An integrated YAC/PAC contig and a partial transcriptional map were constructed by STS-constent mapping using the CEPH YAC library and three PAC libraries. A total of 3 YACs, 34 PACs, and 56 STSs were integrated: 33 newly generated probes to PAC end sequences, 9 polymorphic and 4 nonpolymorphic markers, 5 known genes, 4 expressed sequence tags, and 1 pseudogene. Within the map, a complete PAC contig of > 1 Mb encompasses the IL-1 gene cluster and PAX8, a paired-box-containing gene. This allowed us to define the transcriptional orientation of GLVR1, IL1B, and IL1RN and to show that PAX8 is localized outside the IL-1 gene cluster. FISH analysis localized PAC clones containing the IL-1 gene cluster to 2q12-q13. The data provide the basis for further characterization of the IL-1 gene cluster and for the construction of a sequence-ready PAC contig of this region.
Nothwang
H G
HG
University Children's Hospital, Freiburg University, Germany.
Strahm
B
B
Denich
D
D
Kübler
M
M
Schwabe
J
J
Gingrich
J C
JC
Jauch
A
A
Cox
A
A
Nicklin
M J
MJ
Kurnit
D M
DM
Hildebrandt
F
F
D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007375:Interleukin-1 / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016324:Sequence Tagged Sites
D017931:DNA Primers; D005819:Genetic Markers; D007375:Interleukin-1