Direct analysis of fluoxetine and norfluoxetine in plasma by gas chromatography with nitrogen-phosphorus detection.
A quantitative method for the simultaneous GC resolution and detection of fluoxetine and his metabolite norfluoxetine in human plasma was developed. The procedure required 1.0 ml of plasma, extraction with a mixed organic solvent and injection into a capillary gas chromatograph with an OV-1 fused-silica column coupled to a nitrogen-phosphorus detector. The calibration curves were linear over the range 5-3000 ng/ml. The detection limits were 0.3 and 2 ng/ml for fluoxetine and norfluoxetine, respectively. The assay is suitable for routine analysis.
Fontanille
P
P
Laboratoire de Pharmacologie, Centre Hospitalier Universitaire de Grenoble, France.
Jourdil
N
N
Villier
C
C
Bessard
G
G
D002849:Chromatography, Gas / Q000379:methods*; D005473:Fluoxetine / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D009584:Nitrogen; D010758:Phosphorus; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005473:Fluoxetine; D010758:Phosphorus; C036139:norfluoxetine; D009584:Nitrogen