title: Structure of the human type I iodothyronine 5'-deiodinase gene and localization to chromosome 1p32-p33.
abstract: The human type I iodothyronine 5'-deiodinase gene encodes a member of the family of selenocysteine-containing deiodinases. These enzymes catalyze the activation of the prohormone thyroxine to 3,3',5-triiodothyronine or the degradation of thyroxine and triiodothyronine to inactive metabolites. Here we report the isolation of two genomic type I 5'-deiodinase clones from a chromosome 1-specific gridded cosmid library, the localization of the gene to chromosome 1p32-p33 by fluorescence in situ hybridization, and the determination of the complete structure of the 17.5-kb gene.
authors.affiliation: Medizinische Poliklinik, Klinische Forschergruppe, University of Würzburg, Germany.
authors.forename: T C
authors.initials: TC
authors.lastname: Jakobs
authors.forename: M R
authors.initials: MR
authors.lastname: Koehler
authors.forename: C
authors.initials: C
authors.lastname: Schmutzler
authors.forename: F
authors.initials: F
authors.lastname: Glaser
authors.forename: M
authors.initials: M
authors.lastname: Schmid
authors.forename: J
authors.initials: J
authors.lastname: Köhrle
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D007453:Iodide Peroxidase / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D007453:Iodide Peroxidase