Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible co-factor for epidermal growth factor in cell culture.
Oligosaccharides, involved in regulation of plant developmental and defensive processes, were tested to determine their ability to enhance proliferation of human keratinocytes. A mixture of alginate oligosaccharides remarkably stimulated keratinocyte growth and [3H]thymidine uptake in the presence of epidermal growth factor (EGF). The activity was comparable to bovine pituitary extract, a common complement in keratinocyte culture, and additive on BPE-induced stimulation. The most effective oligosaccharide in the mixture was identified and its chemical structure was determined. These findings demonstrate a novel activity of alginate oligosaccharide(s) in keratinocyte growth and suggest a possible co-factor for EGF-dependent stimulation in medium for keratinocytes.
Kawada
A
A
Department of Dermatology, National Defense Medical College, Tokorozawa, Saitama, Japan.
Hiura
N
N
Shiraiwa
M
M
Tajima
S
S
Hiruma
M
M
Hara
K
K
Ishibashi
A
A
Takahara
H
H
D000464:Alginates / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010902:Pituitary Gland / Q000737:chemistry; D012624:Seaweed / Q000737:chemistry; D013936:Thymidine / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology
D000464:Alginates; D009844:Oligosaccharides; D014020:Tissue Extracts; D004815:Epidermal Growth Factor; D004247:DNA; D013936:Thymidine