Terminal dUTP nick end labeling (TUNEL) positive cells in the different regions of the brain in normal aging and Alzheimer patients.
This study investigated terminal dUTP nick-end labeling (TUNEL)-positive cells in the frontal, occipital, and hippocampal cortices of seven normal aging and four Alzheimer's patients. Significant increase in TUNEL-positive cells was observed in the frontal and hippocampal cortices of Alzheimer's patients when compared with controls. In the hippocampal cortex, only area CA4 demonstrated a significant increase of TUNEL-positive cells. Double staining of TUNEL-positive cells for glial fibrillary acidic protein revealed that < 13% of the TUNEL-positive nuclei belonged to astrocytes. The results of this study illustrated a differential pattern of cortical degeneration between normal aging and Alzheimer patients.
Li
W P
WP
Department of Neurology, First Military Medical University, Guangzhou, China.
Chan
W Y
WY
Lai
H W
HW
Yew
D T
DT
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000473:pathology*; D000544:Alzheimer Disease / Q000401:mortality / Q000473:pathology*; D017209:Apoptosis / Q000502:physiology; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology; D001710:Biotin; D002467:Cell Nucleus / Q000473:pathology; D053938:DNA Fragmentation; D005260:Female; D006801:Humans; D008297:Male; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D013194:Staining and Labeling; D014500:Uracil Nucleotides / Q000032:analysis*
D014500:Uracil Nucleotides; D001710:Biotin