title: Regulation of the DNA binding of p53 by its interaction with protein kinase CK2.
abstract: Some of the numerous functions of the growth suppressor protein p53 are regulated by its interaction with viral and cellular proteins. C-terminal sequences of p53 are implicated in binding to the regulatory beta-subunit of protein kinase CK2. Using a p53-specific DNA binding element we found that the beta-subunit of CK2 inhibited the DNA binding of p53 whereas the alpha-subunit had no influence. The CK2 holoenzyme consisting of two alpha- and two beta-subunits led to a supershift in DNA binding of p53 similar to the p53-specific monoclonal antibody PAb421 as well as the C-terminus of p53. Thus, our results showed an individual role of the free beta-subunit of CK2 on the DNA binding activity of p53.
authors.affiliation: Department of Medical Biochemistry, University of the Saarland, Homburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Prowald
authors.forename: N
authors.initials: N
authors.lastname: Schuster
authors.forename: M
authors.initials: M
authors.lastname: Montenarh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D047390:Casein Kinase II; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006801:Humans; D010766:Phosphorylation; D011487:Protein Conformation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D018411:Spodoptera; D016159:Tumor Suppressor Protein p53 / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D016159:Tumor Suppressor Protein p53; D004247:DNA; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases