title: Diversity of isolates of Acinetobacter from activated sludge systems based on their whole cell protein patterns.
abstract: Whole cell protein extracts from strains of the currently recognized genomic species of Acinetobacter, together with those from a range of isolates of several genomic species identified using the Biolog system and obtained from a biological nutrient-removal activated sludge plant were analysed by SDS-PAGE. The dendrograms obtained after numerical analysis for the known genomic species generally supported the taxonomic relationships suggested from earlier DNA-DNA hybridisation data. In some cases the activated sludge isolates identified to genomic species level clustered closely with the corresponding genomic species reference strains, although isolates 5 and 8/9 were scattered throughout the dendrogram. Considerable variations were seen in the protein patterns of the 27 different environmental isolates of genomic species 7 that were analysed. Three unidentified Acinetobacter isolates examined formed their own subcluster.
authors.affiliation: Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia.
authors.forename: A M
authors.initials: AM
authors.lastname: Maszenan
authors.forename: R J
authors.initials: RJ
authors.lastname: Seviour
authors.forename: B M
authors.initials: BM
authors.lastname: McDougall
authors.forename: J A
authors.initials: JA
authors.lastname: Soddell
mesh_terms: D000150:Acinetobacter / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis*; D016000:Cluster Analysis; D014644:Genetic Variation; D012722:Sewage / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D012722:Sewage