title: [In vitro study of morphology of the bladder wall using MR tomography at 1.0 Tesla: correlation with histology].
abstract: OBJECTIVE
  Evaluation of the potential of various MR techniques to differentiate bladder wall layers verified by histological findings.
  
  
  METHODS
  6 bladder specimens of pigs were examined in vitro using T1-weighted spin-echo-sequences, T2-weighted Turbo-SE, fat suppressed T2-weighted SE, and inversion recovery sequences. The MR images were obtained before and after fixating the specimens in formalin. Measurements of the thickness of bladder wall layers were performed on both sets of MR images as well as on histological sections,
  
  
  RESULTS
  T2-weighted SE images showed three layers of different signal intensities: one innermost band of very high signal, one inner band of low and one outer band of intermediate signal corresponding histologically tunica propria and two different muscle layers. Inversion recovery technique provided similar findings but were able to avoid chemical-shift artifacts. After 24 hours in formalin, the signal intensity relation of the two muscle layers was inverted. The thickness of total bladder wall was not reduced significantly. Concerning the thickness of urinary bladder wall, histological measurements and evaluation of MR images correlated well.
  
  
  CONCLUSIONS
  MR imaging enables the differentiation of three bladder wall layers. Inversion recovery technique achieved the best image quality by avoiding chemical shift artifact.
authors.affiliation: Abteilung für Radiologische Diagnostik, Eberhard-Karls-Universität Tübingen.
authors.forename: F
authors.initials: F
authors.lastname: Teufl
authors.forename: F
authors.initials: F
authors.lastname: Dammann
authors.forename: M
authors.initials: M
authors.lastname: Wehrmann
mesh_terms: D000818:Animals; D005557:Formaldehyde / Q000494:pharmacology; D006652:Histological Techniques; D066298:In Vitro Techniques; D008279:Magnetic Resonance Imaging / Q000379:methods; D008962:Models, Theoretical; D013552:Swine; D013997:Time Factors; D001743:Urinary Bladder / Q000033:anatomy & histology* / Q000187:drug effects
chemical_list: D005557:Formaldehyde