Low-molecular-weight displacers for high-resolution protein separations.
The resolving power of displacement chromatography using low-molecular-weight displacers was investigated using a model mixture containing bovine and horse heart cytochrome c. The linear and nonlinear adsorption behavior of these two proteins was examined in cation-exchange chromatography and shown to be quite similar. Furthermore, an analysis of the dynamic affinity of these proteins indicated extremely similar affinities under displacement conditions. Despite the extreme similarities in the adsorption behavior, displacement chromatography using a protected amino acid displacer resulted in excellent separation of the proteins with both high yields and purity. These results indicate that displacement chromatography may be efficacious for a wide variety of difficult protein separation problems.
Kundu
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Howard P. Isermann Department of Chemical Engineering Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Cramer
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D000327:Adsorption; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives*; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003574:Cytochrome c Group / Q000302:isolation & purification*; D006736:Horses; D016014:Linear Models; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology
D003574:Cytochrome c Group; D001120:Arginine; C009727:benzoylarginine ethyl ester