title: Polyphenol oxidase activity staining in polyacrylamide electrophoresis gels.
abstract: An analytical method allowing the detection of polyphenol oxidase activity on polyacrylamide gel electrophoresis (PAGE) is described. The method is rapid, sensitive and specific and is based on a coupling reaction between 4-tert-butyl-o-benzoquinone and the aromatic amine, 4-amino-N,N-diethylaniline sulphate. Catecholase activity of polyphenol oxidase appears as blue stained bands on a colourless background.
authors.affiliation: Istituto di Chonica Biologica, Università di Cagliari, Italy. rescigno@vaxcal.unica.it
authors.forename: A
authors.initials: A
authors.lastname: Rescigno
authors.forename: F
authors.initials: F
authors.lastname: Sollai
authors.forename: A C
authors.initials: AC
authors.lastname: Rinaldi
authors.forename: G
authors.initials: G
authors.lastname: Soddu
authors.forename: E
authors.initials: E
authors.lastname: Sanjust
mesh_terms: D000818:Animals; D004156:Catechol Oxidase / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D004156:Catechol Oxidase