title: Encapsulation of flavodoxin in reverse micelles.
abstract: The regulation of the properties of Desulfovibrio gigas flavodoxin in AOT/water/iso-octane micellar system was studied. UV-visible spectroscopic studies have shown that photoreduction of flavodoxin in the presence of EDTA leads to hydroquinone formation through the intermediate semiquinone. The [free FMN] - [bound to flavodoxin FMN] equilibrium (and hence, the amount of apoprotein) depends on redox state of FMN and on hydration degree which controls the micellar size. Thus, a new method of reversible cofactor removing under mild conditions (at low hydration degree of micelles) is suggested, accompained by isolation of apo-form of the protein.
authors.affiliation: Departamento de Quimica, Centro de Quimica Fina e Biotecnologia, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, Monte de Caparica, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Andrade
authors.forename: E O
authors.initials: EO
authors.lastname: Kamenskaya
authors.forename: A V
authors.initials: AV
authors.lastname: Levashov
authors.forename: J J
authors.initials: JJ
authors.lastname: Moura
mesh_terms: D003901:Desulfovibrio / Q000737:chemistry; D005418:Flavodoxin / Q000737:chemistry*; D008823:Micelles; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence
chemical_list: D005418:Flavodoxin; D008823:Micelles