Suppression of Helicobacter pylori urease activity by sucralfate and sulglycotide.
The effect of gastroprotective agents, sucralfate and sulglycotide, on the in vitro activity of H. pylori urease was investigated. The bacterium was subjected to sonication, centrifuged, and the supernatant used as an enzyme source. The assays revealed that the rate of urea degradation was proportional to enzyme protein up to 100 micrograms and remained constant with time for 10 min. Introduction of sucralfate or sulglycotide to the assay system led to the reduction in the rate of ammonia production. With both drugs the optimal inhibition was attained at 10 micrograms/ml, at which dose a 63.1% decrease in urease activity occurred with sucralfate and a 70.2% inhibition was obtained with sulglycotide. The findings demonstrate that the inhibitory action of sucralfate and sulglycotide on H. pylori urease activity may be of value in the treatment of gastric disease associated with H. pylori infection.
Slomiany
B L
BL
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400, USA.
Piotrowski
J
J
Slomiany
A
A
D000641:Ammonia / Q000378:metabolism; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002498:Centrifugation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000201:enzymology*; D007700:Kinetics; D012795:Sialoglycoproteins / Q000494:pharmacology*; D013010:Sonication; D013392:Sucralfate / Q000494:pharmacology*; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000037:antagonists & inhibitors*
D000897:Anti-Ulcer Agents; D004791:Enzyme Inhibitors; D012795:Sialoglycoproteins; C009354:sulglicotide; D013392:Sucralfate; D000641:Ammonia; D014508:Urea; D014510:Urease