Detection of hepatitis A virus RNA in oyster meat.
Detection of low concentrations of viruses in shellfish is possible with nucleic acid amplification by PCR. Hepatitis A virus (HAV) has been detected in oyster meat by reverse transcription-PCR (RT-PCR). We developed a method to identify HAV RNA by RT-PCR of total RNA extracted from oyster meat contaminated by adsorption, bioaccumulation, or injection. With dot blot hybridization detection of amplicons from the RT-PCR, rapid screening of a large number of samples is feasible. As few as 8 PFU of HAV/g of oyster meat can be detected.
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Hepatitis Branch (World Health Organization Collaborating Center), National Centers for Disease Control and Prevention, Atlanta, Georgia, USA. TRC1@ciddvd1.em.cdc.gov
Nainan
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Margolis
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D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005069:Evaluation Studies as Topic; D006506:Hepatitis A / Q000517:prevention & control / Q000635:transmission; D006507:Hepatovirus / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D010125:Ostreidae / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D011634:Public Health; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D012623:Seawater / Q000821:virology; D012680:Sensitivity and Specificity; D012722:Sewage / Q000821:virology; D012758:Shellfish / Q000821:virology*
D017931:DNA Primers; D012367:RNA, Viral; D012722:Sewage