Inhibition of lipid peroxidation by N-acetylserotonin and its role in retinal physiology.
N-Acetylserotonin (NAS) inhibits the peroxidation of linoleic acid induced by a water-soluble initiator, 2,2'-azobis(2-amidinopropane) (ABAP). Lipid peroxidation was detected by the formation of conjugated dienes, monitored spectrophotometrically at 236 nm. N-Acetylserotonin, at concentrations ranging from 200 nM to 20 microM, reduced the rate of ABAP-initiated lipid peroxidation in a concentration-dependent manner. The results of NAS-inhibited lipid peroxidation are compared to the antioxidant activities of melatonin, vitamin E, and a water-soluble vitamin E analog, Trolox. It is suggested that NAS acts as a physiological antioxidant in retinal photoreceptor cells.
Longoni
B
B
Dipartimento di Fisiologia e Biochimica, Universitá di Pisa, Italy.
Pryor
W A
WA
Marchiafava
P
P
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002839:Chromans / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008550:Melatonin / Q000494:pharmacology / Q000502:physiology; D010786:Photoreceptor Cells / Q000502:physiology; D010870:Pineal Gland / Q000502:physiology; D012160:Retina / Q000502:physiology*; D012701:Serotonin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000502:physiology; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D002839:Chromans; D016166:Free Radical Scavengers; D014810:Vitamin E; D012701:Serotonin; D008550:Melatonin; C006389:N-acetylserotonin; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid