Capillary electrophoretic analysis of genetic variants of milk proteins from different species.
Polymorphism of bovine, ovine and caprine milk proteins was studied by CE. Identification of some rare bovine variants was carried out by isoelectric focussing (IEF) using PhastSystem. Genetic variants A and D of bovine alpha s2-casein, beta-casein variants A1, A2, A3, B and C and alpha s1-casein variants B and C were determined by CE. In addition, the different casein fractions including some genetic variants of ovine and caprine milk were identified by CE. In order to carry out this identification, collected fractions from a cation-exchange FPLC separation were injected by CE.
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Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain.
Pérez-Rodríguez
M L
ML
Ramos
M
M
Amigo
L
L
D000818:Animals; D002364:Caseins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D002417:Cattle; D002853:Chromatography, Liquid; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D007525:Isoelectric Focusing; D007782:Lactoglobulins / Q000032:analysis / Q000235:genetics; D008894:Milk Proteins / Q000032:analysis* / Q000235:genetics; D012756:Sheep
D002364:Caseins; D007782:Lactoglobulins; D008894:Milk Proteins