Epstein-Barr virus infection of human gastric carcinoma cells: implication of the existence of a new virus receptor different from CD21.
Recombinant Epstein-Barr virus (EBV) with a selectable marker successfully infected the human gastric carcinoma cell lines AGS, MKN28, and MKN74. Following incubation in selective media, drug-resistant cell clones were isolated and proved to be infected with EBV. All gastric carcinoma cell clones were positive for EBNA 1 but negative for EBNA 2. LMP 1 expression was negative in most clones, but there were a few exceptions. Gastric carcinoma cells were negative for the EBV receptor CD21, and infection was not inhibited by pretreatment of cells with the anti-CD21 monoclonal antibody OKB7. The results indicate that gastric carcinoma cells are susceptible to EBV infection and that infection is mediated via a new receptor different from CD21.
Yoshiyama
H
H
Department of Virology, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
Imai
S
S
Shimizu
N
N
Takada
K
K
D002460:Cell Line; D004268:DNA-Binding Proteins / Q000032:analysis; D019309:Epstein-Barr Virus Nuclear Antigens / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D017464:Receptors, Complement 3d / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D013274:Stomach Neoplasms; D015534:Trans-Activators / Q000032:analysis; D014763:Viral Matrix Proteins / Q000378:metabolism; D014764:Viral Proteins; D014779:Virus Replication
C063676:BZLF1 protein, Herpesvirus 4, Human; D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D017464:Receptors, Complement 3d; D011991:Receptors, Virus; D015534:Trans-Activators; D014763:Viral Matrix Proteins; D014764:Viral Proteins