Fasciola hepatica: vaccination of rabbits with native and recombinant antigens related to fatty acid binding proteins.
The current study was designed to compare the immunogenic and immunoprophylactic properties of native (nFh12) and recombinant (rFh15) antigens from Fasciola hepatica in rabbits infected with the fluke. Levels of specific anti-nFh12 and anti-rFh15 antibodies were significantly higher in the rabbits vaccinated twice compared with non-vaccinated infection controls. A reduction of 40% in worm burdens was found in rabbits immunized with nFh12 and infected 4 weeks after the second immunization. The recombinant vaccine induced lesser levels of protection than the native one, suggesting that both molecules may have slight differences either in immunogenicity or in their configuration. Further biochemical studies are required to define these differences. The mean length of flukes recovered was always smaller in all vaccinated rabbits. In addition, infected control rabbits had higher gamma glutamil transferase (GGT) levels than immunized rabbits. Lastly, gross anatomic observation always showed fewer liver lesions in all vaccinated rabbits than in controls. This finding clearly supports the possibility of vaccination regimes in fasciolosis.
Muro
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A
Unidad Patología Animal, Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Salamanca, Spain. ama@gugu.usal.es
Ramajo
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López
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J
Simón
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Hillyer
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GV
D000818:Animals; D000909:Antibodies, Helminth / Q000302:isolation & purification; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005211:Fascioliasis / Q000276:immunology* / Q000517:prevention & control; D050556:Fatty Acid-Binding Proteins; D007115:Immunization Schedule; D008099:Liver / Q000201:enzymology / Q000473:pathology; D018996:Myelin P2 Protein / Q000276:immunology*; D009363:Neoplasm Proteins; D011817:Rabbits; D014611:Vaccination; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic