Ribosomal 5 S rRNA maturation in Saccharomyces cerevisiae.
The maturation of the ribosomal 5 S RNA in Saccharomyces cerevisiae is examined based on the expression of mutant 5 S rRNA genes, in vivo, and a parallel analysis of RNA processing, in vitro. Both types of analysis indicate that 5 S rRNA processing is not dependent on the nucleotide sequence of either the external transcribed spacer or the mature 5 S rRNA. The results further indicate the RNA is processed by an exonuclease activity which is limited primarily or entirely by helix I, the secondary structure formed between the mature and interacting termini. The 5 S RNA binding protein (YL3) also appears not to influence directly the maturation process, but rather to play a role in protecting the rRNA from further degradation by "housekeeping" nucleases. Taken together, the results continue to support a "quality control" function which helps to ensure that during maturation only normal precursors are processed and assembled into active ribosomes.
Lee
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Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Nazar
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D009690:Nucleic Acid Conformation; D012323:RNA Processing, Post-Transcriptional; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012341:RNA, Ribosomal, 5S