title: Isolation and characterization of the mycobacterial phagosome: segregation from the endosomal/lysosomal pathway.
abstract: Mycobacteria have the ability to persist within host phagocytes, and their success as intracellular pathogens is thought to be related to the ability to modify their intracellular environment. After entry into phagocytes, mycobacteria-containing phagosomes acquire markers for the endosomal pathway, but do not fuse with lysosomes. The molecular machinery that is involved in the entry and survival of mycobacteria in host cells is poorly characterized. Here we describe the use of organelle electrophoresis to study the uptake of Mycobacterium bovis bacille Calmette Guerin (BCG) into murine macrophages. We demonstrate that live, but not dead, mycobacteria occupy a phagosome that can be physically separated from endosomal/lysosomal compartments. Biochemical analysis of purified mycobacterial phagosomes revealed the absence of endosomal/lysosomal markers LAMP-1 and beta-hexosaminidase. Combining subcellular fractionation with two-dimensional gel electrophoresis, we found that a set of host proteins was present in phagosomes that were absent from endosomal/lysosomal compartments. The residence of mycobacteria in compartments outside the endosomal/lysosomal system may explain their persistence inside host cells and their sequestration from immune recognition. Furthermore, the approach described here may contribute to an improved understanding of the molecular mechanisms that determine the intracellular fate of mycobacteria during infection.
authors.affiliation: Department of Medical Microbiology, Imperial College School of Medicine at St. Mary's, London, UK.
authors.forename: Z
authors.initials: Z
authors.lastname: Hasan
authors.forename: C
authors.initials: C
authors.lastname: Schlax
authors.forename: L
authors.initials: L
authors.lastname: Kuhn
authors.forename: I
authors.initials: I
authors.lastname: Lefkovits
authors.forename: D
authors.initials: D
authors.lastname: Young
authors.forename: J
authors.initials: J
authors.lastname: Thole
authors.forename: J
authors.initials: J
authors.lastname: Pieters
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D002458:Cell Fractionation; D002460:Cell Line; D011992:Endosomes / Q000382:microbiology; D008247:Lysosomes / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D009163:Mycobacterium bovis / Q000302:isolation & purification / Q000472:pathogenicity*; D010588:Phagosomes / Q000382:microbiology*