title: [Experimental validation of a semi-quantitative single-layer procedure for proton-spin tomographic imaging of regional cerebral perfusion].
abstract: OBJECTIVE
  The aim of the present study was to validate a simple MRI-procedure for semiquantitative assessment of regional cerebral blood flow.
  
  
  METHODS
  Unilateral cerebral ischaemia (30 minutes) in the territory of the middle cerebral artery was induced in 14 anesthetised rates. The MRI-experiment consisted in an intravenous bolus injection of gadolinium-DTPA, recording of the cerebral contrast kinetics with a T2*-weighted pulse sequence, and measurement of the maximal concentration change at a chosen reference point of time. To measure perfusion quantitatively, a microsphere technique, an accepted reference technique was used. With both methods a perfusion index related to the contralateral side was calculated.
  
  
  RESULTS
  In all cases decreased perfusion was detected by the MRI technique. The perfusion indices correlated with a coefficient of correlation of r = 0.89 (p < 0.001).
  
  
  CONCLUSIONS
  The results demonstrate that contrast-enhanced MRI with bolus injection can be implemented with clinical potential as a semiquantitative instrument for the assessment of cerebral perfusion. Regional cerebral blood volume and collateral blood flow may interfere with the estimate of blood flow.
authors.affiliation: Strahlenklinik und Poliklinik, Virchow-Klinikum, Medizinische Fakultät, Humboldt-Universität zu Berlin.
authors.forename: J C
authors.initials: JC
authors.lastname: Böck
authors.forename: A H
authors.initials: AH
authors.lastname: Goetze
authors.forename: R
authors.initials: R
authors.lastname: Felix
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply*; D002545:Brain Ischemia / Q000473:pathology*; D002536:Cerebral Arteries; D002560:Cerebrovascular Circulation; D003287:Contrast Media; D005682:Gadolinium / Q000008:administration & dosage; D019786:Gadolinium DTPA; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008863:Microspheres; D009942:Organometallic Compounds / Q000008:administration & dosage; D004369:Pentetic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003287:Contrast Media; D009942:Organometallic Compounds; D004369:Pentetic Acid; D005682:Gadolinium; D019786:Gadolinium DTPA