title: Parasite antigen-induced IFN-gamma and IL-4 production by cells from pathopermissive and pathoresistant strains of mice infected with Trypanosoma cruzi.
abstract: The cardiac pathology associated with infection by Trypanosoma cruzi in mice has been suggested to be partially dependent upon cytokine responses. The pathoresistant B10.D2 mice, which display little infection-induced myocarditis, and the pathopermissive DBA/2 mice, which show significant cardiac damage, were compared for their in vitro interferon (IFN)-gamma and interleukin (IL)-4 production. Concanavalin A-stimulated spleen cells from infected B10.D2 mice produce a greater amount of IFN-gamma than DBA/2 mice, whereas the IL-4 production is only slightly greater in the B10.D2 mice than the DBA/2 mice. Parasite antigen stimulation of spleen cells from these mice results in a clearly greater IFN gamma production by the B10.D2 and a higher IL-4 level for the DBA/2 mice. The data presented suggest a relationship between an enhanced TH1-type response and decreased chronic cardiac pathogenesis, whereas a lower level of TH1 activity may play a role in cardiac involvement.
authors.affiliation: Tropical Disease Institute, College of Osteopathic Medicine, Ohio University, Athens 45701, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Humphrey
authors.forename: T S
authors.initials: TS
authors.lastname: McCormick
authors.forename: E C
authors.initials: EC
authors.lastname: Rowland
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D002478:Cells, Cultured; D002598:Chagas Cardiomyopathy / Q000276:immunology; D014355:Chagas Disease / Q000276:immunology*; D003208:Concanavalin A; D004198:Disease Susceptibility; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D018512:Parasitemia / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D003208:Concanavalin A; D015847:Interleukin-4; D007371:Interferon-gamma