In vitro release kinetics of biologically active transforming growth factor-beta 1 from a novel porous glass carrier.
Sol-gel silica-based porous glass (xerogel) was used as a novel carrier material for recombinant human transforming growth factor-beta 1 (TGF-beta 1). Room temperature synthesis procedures included sol preparation, the addition of TGF-beta 1 solution to the sol, subsequent gelation and drying. After determination of optimal synthesis parameters, the material was assayed in vitro for its ability to release biologically active TGF-beta 1 in a controlled manner. Sustained release of TGF-beta 1 over a 7-day period was demonstrated. On the basis of published TGF-beta 1 potency, the amount released is capable of eliciting bone tissue reactivity. These findings suggest that this novel glass-growth factor composite may serve as an effective bone graft material for the repair of osseous defects.
Nicoll
S B
SB
Department of Bioengineering, University of Pennsylvania, Philadelphia 19104, USA.
Radin
S
S
Santos
E M
EM
Tuan
R S
RS
Ducheyne
P
P
D001672:Biocompatible Materials; D018786:Bone Substitutes; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005782:Gels; D005898:Glass; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D010012:Osteogenesis / Q000187:drug effects; D010865:Pilot Projects; D011994:Recombinant Proteins / Q000008:administration & dosage; D016212:Transforming Growth Factor beta / Q000008:administration & dosage*
D001672:Biocompatible Materials; D018786:Bone Substitutes; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005782:Gels; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta