Calcitonin gene-related peptide and alpha-CGRP mRNA expression in cranial motoneurons after hypoglossal nerve injury during postnatal development.
Changes in calcitonin gene-related peptide (CGRP) immunoreactivity and alpha-CGRP mRNA expression were determined in the hypoglossal nucleus after the nerve was crushed or transected in rats at 10, 14 and 21 days postnatal. alpha-CGRP mRNA expression was determined in normal, noninjured, hypoglossal nuclei at the three ages and after both injuries in 10 and 21 days postnatal rats. Reinnervation and neuronal survival were assayed. Although the three age groups expressed comparable levels of alpha-CGRP mRNA and its peptide in intact, hypoglossal nuclei, axonal injury produced age-dependent alterations in alpha-CGRP mRNA and CGRP. In the 21 days postnatal rats, changes in alpha-CGRP mRNA and peptide mimicked those reported in adult motoneurons after the same injuries. CGRP was elevated until reinnervation after nerve crush, whereas biphasic elevations occurred after nerve transection. In 21 days postnatal rats, increases in alpha-CGRP mRNA preceded elevations of the peptide but a greater increase resulted initially after nerve transection. An upregulation of alpha-CGRP mRNA also developed initially after both injuries in 10 days postnatal rats but subsequent elevations of alpha-CGRP mRNA did not materialize. In contrast, CGRP immunoreactivity did not increase after either injury in 10 days postnatal rats and, in fact decreased. Levels of CGRP immunoreactivity did not differ from normal amounts after either nerve injury in 14 days postnatal rats. Substantial neuronal cell loss occurred after each injury in 10 and 14 days postnatal rats but was not found in 21 days postnatal rats. Tongue reinnervation by surviving motoneurons was established after all injury paradigms except 10 days postnatal transection. The current findings demonstrate an age-dependent correlation between injury-induced expression of CGRP and hypoglossal motoneuron survival.
Blake-Bruzzini
K M
KM
Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Borke
R C
RC
Anders
J J
JJ
Potts
J D
JD
D000375:Aging; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics* / Q000378:metabolism*; D002470:Cell Survival; D003714:Denervation; D015870:Gene Expression; D007002:Hypoglossal Nerve / Q000254:growth & development / Q000502:physiology* / Q000601:surgery; D009046:Motor Neurons / Q000378:metabolism*; D009409:Nerve Crush; D009416:Nerve Regeneration; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012260:Ribonucleases; D014059:Tongue / Q000294:innervation
D012333:RNA, Messenger; D012260:Ribonucleases; D015740:Calcitonin Gene-Related Peptide