No evidence of KSHV/HHV-8 in mycosis fungoides or associated disorders.
The recently discovered human virus known as Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus-8 (HHV-8) has been associated with body-cavity-based lymphomas in AIDS patients. It is most closely related to two other herpesviruses, the Epstein-Barr virus and herpesvirus saimiri, which are known to be associated with lymphomas in humans and nonhuman primates, respectively. To determine whether KSHV/HHV-8 is involved in the pathogenesis of mycosis fungoides (MF) and related disorders, we used a genomic PCR assay followed by confirmatory Southern blot analysis with a nested oligonucleotide probe to analyze cases for the presence of this virus. The specimens studied included fresh-frozen lesional tissues obtained from 16 patients with MF, seven with lymphomatoid papulosis, seven with primary cutaneous CD30+ large cell lymphoma of T-cell lineage, and five with Hodgkin's disease. Two T-cell tumor lines were also studied: MT4 (derived from a patient with adult T-cell leukemia/lymphoma) and Jurkat (derived from a patient with T-cell acute lymphoblastic leukemia). All cases were uniformly negative for KSHV/HHV-8, whereas Kaposi's sarcoma-positive controls and human beta-globin DNA integrity controls were appropriately positive. These findings provide strong evidence against a role for KSHV/HHV-8 in the pathogenesis of MF or associated lymphoproliferative disorders.
Henghold
W B
WB
Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
Purvis
S F
SF
Schaffer
J
J
Giam
C Z
CZ
Wood
G S
GS
D000328:Adult; D001483:Base Sequence; D001609:Beta-Globulins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D015139:Blotting, Southern; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006689:Hodgkin Disease / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D006801:Humans; D019169:Jurkat Cells / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry / Q000473:pathology / Q000821:virology*; D009182:Mycosis Fungoides / Q000737:chemistry / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012867:Skin / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D017444:Skin Diseases, Papulosquamous / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D012878:Skin Neoplasms / Q000737:chemistry / Q000473:pathology / Q000821:virology*; D014407:Tumor Cells, Cultured
D001609:Beta-Globulins; D017931:DNA Primers; D004273:DNA, Neoplasm; D004279:DNA, Viral; D004247:DNA