Cell cultures and associated retroviruses in multiple sclerosis. Collaborative Research Group on MS.
Retroviral particles associated with reverse transcriptase (RT) activity in cell-cultures from MS patients have been reported by different groups. Cell-cultures have been used for the study and characterization of the corresponding retroviral genome which we have shown is related to ERV9 in the pol region. Previously unpublished details of a study with monocyte cultures are presented together with observations on leptomeningeal and choroid-plexus cultures. The generation of self-transformed cultures after inhibition of interferon, followed by the loss of retroviral expression and recurrent apoptosis, is analyzed. Retroviral particles with RT-activity are produced in monocyte cultures with an apparent correlation with MS disease activity. However, though leptomeningeal and choroid plexus cells from MS can be passaged for a limited period, their evolution in vitro is not compatible with stable retroviral expression. These culture limitations greatly hampered progress on the elucidation of the retroviral genome sequence.
Perron
H
H
BioMérieux S.A., UMR 103 CNRS-bioMérieux, Lyon, France.
Firouzi
R
R
Tuke
P
P
Garson
J A
JA
Michel
M
M
Beseme
F
F
Bedin
F
F
Mallet
F
F
Marcel
E
E
Seigneurin
J M
JM
Mandrand
B
B
D000328:Adult; D000368:Aged; D000818:Animals; D002455:Cell Division / Q000235:genetics; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002472:Cell Transformation, Viral / Q000235:genetics; D002478:Cells, Cultured / Q000821:virology; D002831:Choroid Plexus / Q000821:virology; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D015751:Genes, pol / Q000235:genetics; D006801:Humans; D008297:Male; D008578:Meninges / Q000821:virology; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009000:Monocytes / Q000821:virology*; D009103:Multiple Sclerosis / Q000821:virology*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D012192:Retroviridae Infections / Q000235:genetics / Q000821:virology*
D012194:RNA-Directed DNA Polymerase