title: BstAPI, an ApaBI isoschizomer, cleaves DNA at 5'-GCANNNN NTGC-3'.
abstract: Cleavage positions of Bst API, a new restriction endonuclease (ENase) that recognizes palindromic interrupted DNA sequence, have been determined. Recognition sequences and cleavage sites comparison shows that Bst API shares similarity with a number of type II restriction enzymes.
authors.affiliation: SibEnzyme, 8 Lavrentiev Str., Novosibirsk 630090, Russia. info@siben.nsk.su
authors.forename: M A
authors.initials: MA
authors.lastname: Abdurashitov
authors.forename: O A
authors.initials: OA
authors.lastname: Belichenko
authors.forename: A V
authors.initials: AV
authors.lastname: Shevchenko
authors.forename: V S
authors.initials: VS
authors.lastname: Dedkov
authors.forename: S K
authors.initials: SK
authors.lastname: Degtyarev
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C080472:GCANNNNNTGC -specific type II deoxyribonucleases