title: The adaptability of Escherichia coli thioredoxin to non-conservative amino acid substitutions.
abstract: The adaptability of Escherichia coli thioredoxin to the substitution of a series of non-natural amino acids has been investigated. Different thiosulfonated alkyl groups were inserted into the hydrophobic core of the protein in position 78 via disulfide bonding with a buried cysteine residue as previously described (Wynn R, Richards FM. 1993. Unnatural amino acid packing mutants of Escherichia coli thioredoxin produced by combined mutagenesis/chemical modification techniques. Protein Sci 2:395-403). The side chains added to the cysteine included methyl, ethyl, n-propyl, n-butyl, n-pentyl, and cyclo-pentyl derivatives. The side chains appear to exploit the presence of the large cavities to incorporate these variant forms, enabling the protein to fold and have some activity. Solution structural and kinetic data suggested that these substitutions had little effect on the overall fold of the protein. Thermodynamic data revealed that the entropic effect of restricting the side chains in the folded protein has an effect on the stability. The variant forms of thioredoxin have different propensities to form dimers despite the limited structural perturbations. Molecular modeling studies allow the conformation of the side chains to be assessed.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, United Kingdom.
authors.forename: R
authors.initials: R
authors.lastname: O'Brien
authors.forename: R
authors.initials: R
authors.lastname: Wynn
authors.forename: P C
authors.initials: PC
authors.lastname: Driscoll
authors.forename: B
authors.initials: B
authors.lastname: Davis
authors.forename: K W
authors.initials: KW
authors.lastname: Plaxco
authors.forename: J M
authors.initials: JM
authors.lastname: Sturtevant
authors.forename: J E
authors.initials: JE
authors.lastname: Ladbury
mesh_terms: D002152:Calorimetry, Differential Scanning; D003198:Computer Simulation; D003545:Cysteine / Q000031:analogs & derivatives; D019281:Dimerization; D004926:Escherichia coli / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D013816:Thermodynamics; D013879:Thioredoxins / Q000737:chemistry* / Q000235:genetics
chemical_list: D013879:Thioredoxins; D003545:Cysteine