Inhibition of matrix metalloproteinase-1 by dichloromethylene bisphosphonate (clodronate).
Interstitial collagenase present in human jaw cyst extract and purified human fibroblast-type collagenase (MMP-1) were both efficiently inhibited in vitro by clodronate, an osteoactive, antiresorptive bisphosphonate. The IC50 of clodronate to inhibit MMP-1 is 150 microM. These findings suggest an extended and hitherto undescribed properties for clodronate/biphosphonates in prevention and treatment of tissue degradation in both bone and soft tissue destructive diseases.
Teronen
O
O
Department of Oral and Maxillofacial Surgery, Institute of Dentistry, University of Helsinki, P.O. Bos 41 (Mannerheimintie 172) FIN-00014, Helsinki, Finland.
Konttinen
Y T
YT
Lindqvist
C
C
Salo
T
T
Ingman
T
T
Lauhio
A
A
Ding
Y
Y
Santavirta
S
S
Valleala
H
H
Sorsa
T
T
D001847:Bone Diseases / Q000188:drug therapy; D004002:Clodronic Acid / Q000627:therapeutic use / Q000633:toxicity*; D003094:Collagen / Q000378:metabolism; D017364:Collagenases / Q000302:isolation & purification / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007570:Jaw Cysts / Q000201:enzymology; D020781:Matrix Metalloproteinase 1; D020784:Matrix Metalloproteinase 8; D061965:Matrix Metalloproteinase Inhibitors
D061965:Matrix Metalloproteinase Inhibitors; D004002:Clodronic Acid; D003094:Collagen; D017364:Collagenases; D020784:Matrix Metalloproteinase 8; D020781:Matrix Metalloproteinase 1