title: cDNA sequence analysis of cardiotoxin variants from Taiwan cobra.
abstract: Five cDNAs encoding cardiotoxin variants were constructed from the cellular RNA isolated from the venom glands of Naja naja atra by reverse transcription-polymerase chain reaction. A high degree of nucleotide sequence homology was observed between these variants and other determined ones. Among them, a novel cardiotoxin 6 had 61 amino acid residues rather than 60 ones that usually observed with Naja naja atra cardiotoxins. The other cardiotoxin variants were the homologues of cardiotoxins 1, V or N with one or two amino acid substitutions, respectively. These results probably reflect the involvement of RNA editing in the production of cardiotoxin variants in the venom of Taiwan cobra.
authors.affiliation: Department of Biochemistry, Kaohsiung Medical College, Taiwan, ROC. lschang@mail.nsysu.edu.tw
authors.forename: L S
authors.initials: LS
authors.lastname: Chang
authors.forename: J
authors.initials: J
authors.lastname: Lin
authors.forename: P F
authors.initials: PF
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004179:Cobra Cardiotoxin Proteins / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry*; D004546:Elapid Venoms / Q000737:chemistry*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004179:Cobra Cardiotoxin Proteins; D018076:DNA, Complementary; D004546:Elapid Venoms; C082541:cardiotoxin V; D012313:RNA