Demonstration of common and stage-specific anti-Hepatozoon mocassini antibodies in experimentally infected unnatural lizard hosts.
This study examines the immunological response to Hepatozoon mocassini in lizards. Three lizard species were infected experimentally with H. mocassini. Baseline and post-infection (PI) sera were assayed for anti-H. mocassini meront and gametocyte antibody by immunohistochemistry and IFA. Seroconversion occurred at 38 d PI with endpoint IFA titers of 1:64. Antisera from non-parasitaemic and parisitaemic lizards exhibited similar affinities for merozite and gametocyte antigens. Antibody specific for the membranes of gametocyte-infected erythrocytes was demonstrated exclusively in parasitaemic lizards. The results demonstrate that lizards infected with snake haemogregarines mount an antibody response with common and stage-specific components.
Wozniak
E J
EJ
Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907, USA.
Kanitz
C
C
Homer
B
B
Kreisle
R
R
Telford
S R
SR
McLaughlin
G
G
D000330:Aedes / Q000469:parasitology; D017836:Agkistrodon / Q000469:parasitology; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D016783:Eucoccidiida / Q000276:immunology*; D007150:Immunohistochemistry; D008116:Lizards / Q000276:immunology* / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D018512:Parasitemia / Q000276:immunology; D013045:Species Specificity
D000913:Antibodies, Protozoan