Low specificity of PGP9.5 expression for detection of micrometastatic neuroblastoma.
To determine the specificity of neuroendocrine protein gene product (PGP9.5) gene transcripts for detecting micrometastatic neuroblastoma, we have used a highly sensitive polymerase chain reaction (PCR) technique to evaluate expression of this gene in normal blood and bone marrow. While expression of the tyrosine hydroxylase gene was not detected in any normal sample, low-level PGP9.5 expression was detected in eight out of ten blood and seven of 12 marrow samples. PGP9.5 gene transcripts in normal tissues have the potential to interfere with the detection of micrometastatic neuroblastoma.
Gilbert
J
J
Children's Cancer Research Institute, Sydney Children's Hospital, New South Wales, Australia.
Norris
M D
MD
Marshall
G M
GM
Haber
M
M
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D001483:Base Sequence; D001853:Bone Marrow / Q000737:chemistry; D002460:Cell Line; D005069:Evaluation Studies as Topic; D006801:Humans; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000175:diagnosis* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009447:Neuroblastoma / Q000175:diagnosis / Q000235:genetics* / Q000556:secondary; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013869:Thiolester Hydrolases / Q000235:genetics; D014158:Transcription, Genetic; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D009419:Nerve Tissue Proteins; D014446:Tyrosine 3-Monooxygenase; D013869:Thiolester Hydrolases