title: Effects of inotropes on human leucocyte numbers, neutrophil function and lymphocyte subtypes.
abstract: We have investigated the effects of inotropes with different adrenergic receptor specificity on differential white cell count, lymphocyte subtypes and neutrophil function in healthy volunteers. Six healthy, male volunteers were enrolled into this randomized, placebo-controlled pilot study. Each volunteer was studied on four separate occasions during a 2-h infusion of various agents, and for 2 h after stopping the infusion. The agents investigated were adrenaline 0.1 microgram kg-1 min-1, dobutamine 5 micrograms kg-1 min-1, dopexamine 2 micrograms kg-1 min-1 and 5% glucose 0.5 ml kg-1 h-1. Venous blood was sampled at 0, 30, 120 and 240 min. Haemodynamic monitoring was continued throughout the study. Full blood count, white cell differential count and enumeration of lymphocyte subtypes were performed. Neutrophil function tests included chemoluminescence, and assessment of neutrophil chemotaxis, phagocytosis and adhesion. The Wilcoxon signed rank test was used to compare differences between placebo and active drugs at each time compared with baseline. There was a significant increase in white cell count, lymphocyte count and neutrophil count with adrenaline, and a small but significant decrease in these variables with dobutamine and dopexamine. These changes were also apparent for absolute CD3+, CD4+ and CD8+ lymphocyte counts. Neutrophil respiratory burst in response to f-methionyl-leucyl-phenylalanine increased significantly only with adrenaline at 30 min (P = 0.046). There were no other significant changes in tests of neutrophil function. Infusion of inotropes was associated with changes in white cell numbers, lymphocyte subtypes and neutrophil respiratory burst. In healthy volunteers, adrenaline had effects different from those of dobutamine and dopexamine. The clinical relevance of such effects requires further investigation in critically ill patients.
authors.affiliation: John Farman Intensive Care Unit, Addenbrooke's Hospial, Cambridge.
authors.forename: A M
authors.initials: AM
authors.lastname: Burns
authors.forename: M
authors.initials: M
authors.lastname: Keogan
authors.forename: M
authors.initials: M
authors.lastname: Donaldson
authors.forename: D L
authors.initials: DL
authors.lastname: Brown
authors.forename: G R
authors.initials: GR
authors.lastname: Park
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D002395:Catecholamines / Q000494:pharmacology*; D004280:Dobutamine / Q000494:pharmacology; D004298:Dopamine / Q000031:analogs & derivatives / Q000494:pharmacology; D018491:Dopamine Agonists / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007958:Leukocyte Count / Q000187:drug effects; D016131:Lymphocyte Subsets / Q000187:drug effects*; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010865:Pilot Projects
chemical_list: D000318:Adrenergic beta-Agonists; D002395:Catecholamines; D018491:Dopamine Agonists; C046318:dopexamine; D004280:Dobutamine; D005947:Glucose; D004298:Dopamine; D004837:Epinephrine