Optimization of haemolysis in enhanced haemolysis agar (EHA)--a selective medium for the isolation of Listeria monocytogenes.
The presence of Listeria monocytogenes in enrichment media can be masked by faster growth of other Listeria spp. Therefore, enhanced haemolysis agar (EHA) is a good alternative for another isolation media, because the presence of a few L. monocytogenes colonies can be detected in a majority of colonies of other listeriae on the basis of haemolysis. In this study the haemolysis reaction in EHA was optimized. In a collaborative study using reference samples, no significant differences in counts on EHA, Palcam and Oxford agar were shown.
Beumer
R R
RR
Laboratory of Food Microbiology and Hygiene, Agricultural University, Wageningen, The Netherlands.
te Giffel
M C
MC
Cox
L J
LJ
D000362:Agar; D001431:Bacteriological Techniques / Q000592:standards; D015169:Colony Count, Microbial; D005069:Evaluation Studies as Topic; D006461:Hemolysis; D008089:Listeria monocytogenes / Q000254:growth & development / Q000302:isolation & purification*; D012015:Reference Standards
D000362:Agar