HSP90alpha gene expression may be a conserved feature of vertebrate somitogenesis.
We have previously demonstrated that the hsp90alpha and hsp90beta genes in zebrafish are expressed in dramatically different spatial and temporal patterns in early embryos. In the case of hsp90alpha, expression is spatially restricted within the somites to putative myogenic cells which also express mRNA encoding the myogenic bHLH transcription factor myoD and is downregulated along with myoD following myogenesis. In the present study, we have examined hsp90alpha gene expression in developing chicken embryos using a gene-specific probe. We show that hsp90alpha gene expression is also localized to a subset of cells within the somites of chicken embryos and that the expression pattern correlates closely to that observed for myoD. Furthermore, expression of the hsp90alpha gene is strongly upregulated throughout the embryo following heat shock in a manner similar to that observed in heat-shocked zebrafish embryos. The data suggest that the hsp90alpha gene may play an evolutionarily conserved role during somitogenesis in vertebrates in addition to providing protection to all cells of the embryo following stress.
Sass
J B
JB
Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Canada.
Krone
P H
PH
D000818:Animals; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D009024:Morphogenesis; D009124:Muscle Proteins / Q000235:genetics; D009132:Muscles / Q000196:embryology*; D017570:MyoD Protein / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D019170:Somites / Q000502:physiology*
D018841:HSP90 Heat-Shock Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; D012333:RNA, Messenger