Molecular analysis of hus1+, a fission yeast gene required for S-M and DNA damage checkpoints.
The structure of hus1+, a Schizosaccharomyces pombe gene required for S-M and DNA damage checkpoints, has been determined. Expression of hus1+ requires splicing of five exons, including a microexon that is only 13 nucleotides long. hus1+ is predicted to encode a 33 kDa protein with no similarity to sequences in any database, including the entire S. cerevisiae genome. Yeast strains disrupted for the hus1+ gene are viable but checkpoint-defective. Polyclonal antibodies were raised against bacterially expressed Hus1 protein, and used to study Hus1 regulation. Hus1 protein levels are not affected by S-phase arrest, and are not altered by mutations in other checkpoint genes, suggesting that Hus1 is not regulated at the transcriptional or translational levels.
Kostrub
C F
CF
MRC Cell Mutation Unit, Sussex University, Falmer, UK.
al-Khodairy
F
F
Ghazizadeh
H
H
Carr
A M
AM
Enoch
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004249:DNA Damage / Q000235:genetics*; D018076:DNA, Complementary; D005091:Exons / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005800:Genes, Fungal; D007438:Introns / Q000235:genetics; D008856:Microscopy, Fluorescence; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016366:Open Reading Frames; D016196:S Phase; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D014158:Transcription, Genetic / Q000235:genetics
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D029702:Schizosaccharomyces pombe Proteins; C106332:hus1 protein, S pombe