title: Sarcoma cells engineered to secrete IFN-gamma or IL-2 acquire sensitization to immune cell killing via different mechanisms.
abstract: The murine fibrosarcoma FS29 can be more efficiently killed by syngeneic lymphocytes when it has been engineered to secrete either interferon gamma (IFN-gamma), or interleukin 2 (IL-2). The mechanisms by which the two cytokines enhance target sensitivity differ. Supernatant from IFN-gamma-secreting cells can enhance the sensitivity of unmodified cells. The enhanced sensitivity correlates with MHC upregulation observed on both the IFN-gamma-secreting and supernatant-treated cells. In contrast, supernatant from IL-2-secreting cells does not affect the sensitivity of unmodified cells. IL-2 can be detected, by a bioassay, bound to the extracellular matrix of the secreting tumour cells.
authors.affiliation: CRC Centre for Cell and Molecular Biology, Chester Beatty Laboratories, London.
authors.forename: C L
authors.initials: CL
authors.lastname: Flemming
authors.forename: P M
authors.initials: PM
authors.lastname: Patel
authors.forename: G
authors.initials: G
authors.lastname: Box
authors.forename: S A
authors.initials: SA
authors.lastname: Eccles
authors.forename: M K
authors.initials: MK
authors.lastname: Collins
mesh_terms: D000818:Animals; D016923:Cell Death; D017690:Cell Transplantation; D003602:Cytotoxicity, Immunologic; D005354:Fibrosarcoma; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D015395:Histocompatibility Antigens Class I; D007376:Interleukin-2; D007371:Interferon-gamma