Intranasal immunization of mice with Echinococcus granulosus surface antigens iscoms evokes a strong immune response, biased towards glucidic epitopes.
The present work describes the preparation and characterization of iscoms from tegumental antigens of Echinococcus granulosus protoscoleces, and their use as immunogens in mice by intranasal and subcutaneous routes. Iscoms given at 10 mg doses evoke a significant antibody response when administered i.n. and a strong and long lasting antibody response by s.c. route. The Ag administered i.n. in a non-adjuvanted form as a booster was not immunogenic. The i.n. route of immunization induced higher IgA serum titre in relation to IgG than the s.c. route and antisera of slightly higher avidity. Also, ten fold lower IgG2a/IgG1 and ten fold higher IgG3/IgG1 ratios were observed for the i.n. protocol compared to the s.c. one. Moreover, the mucosal route of immunization induced more efficiently antibodies of both isotypes directed to carbohydrate epitopes. The differences between immune response generated by i.n. and parenteral immunizations should be taken into consideration, particularly in those cases in which protective antigens are glycosylated.
Carol
H
H
Cátedra de Inmunología, Facultad de Química, Montevideo, Uruguay.
Nieto
A
A
Villacres-Eriksson
M
M
Morein
B
B
D000281:Administration, Intranasal; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D002241:Carbohydrates / Q000276:immunology; D004446:Echinococcus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D016882:ISCOMs / Q000276:immunology*; D007114:Immunization / Q000379:methods; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008297:Male; D051379:Mice
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D002241:Carbohydrates; D000939:Epitopes; D016882:ISCOMs; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M