Mouse RT6 locus 1 and rat RT6.2 are NAD+. Arginine ADP-ribosyltransferases with auto-ADP-ribosylation activity.
We report that rat RT6.2 and recombinant mouse Rt6 locus 1 proteins possess auto-ADP-ribosyltransferase activity and that Rt6, but not RT6, catalyzes the ADP-ribosylation of exogenous histones. Based on NH2OH sensitivity, it appeared that the ADP-ribose was attached to arginine residues on proteins. We also observed that the NAD+ concentration in culture medium correlates inversely with the proliferation of rat RT6+ T cells. The data suggest that lymphocyte surface ADP-ribosyltransferases could be involved in signaling and immunoregulatory processes.
Bortell
R
R
Diabetes Division, University of Massachusetts Medical School, Worcester 01605, USA.
Rigby
M
M
Stevens
L
L
Moss
J
J
Kanaitsuka
T
T
Mordes
J
J
Greiner
D
D
Rossini
A
A
D036002:ADP Ribose Transferases / Q000235:genetics / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D002384:Catalysis; D002455:Cell Division; D002460:Cell Line; D008040:Genetic Linkage; D006649:Histocompatibility Antigens / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D009243:NAD / Q000378:metabolism* / Q000494:pharmacology; D011233:Precipitin Tests; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology
D000945:Antigens, Differentiation, T-Lymphocyte; D006649:Histocompatibility Antigens; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D009243:NAD; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; C449339:Art2b protein, mouse; C090163:Art2b protein, rat