title: Modulation of endothelin-induced intracellular Ca2+ mobilization by interleukin-1 beta and lipopolysaccharide in C6 rat glioma cells.
abstract: We have investigated the effects of interleukin (IL)-1 beta and lipopolysaccharide (LPS) on endothelin (ET)-induced intracellular Ca2+ rise in C6 rat glioma cells in order to study the mechanisms of their effects on Ca2+ signaling systems. Pretreatment with IL-1 beta (10(3) U/mL) and LPS (1 microgram/mL) for 24 h significantly inhibited 100 nM ET-1-induced increase in intracellular Ca2+ either in the presence or absence of external Ca2+. Their inhibitory effects were in dosedependent (IL-1 beta; 50-1000 U/mL, LPS; 10-1000 ng/mL) and time-dependent (12-24 h) manners. A tyrosine kinase antagonist genistein (50 microM) but not a protein kinase C inhibitor H7 (30 microM) prevented the inhibition of the ET response by IL-1 beta and LPS. These results suggest that activation of tyrosine kinase may be essential for the inhibition of the ET receptor-mediated Ca2+ signaling systems by IL-1 beta and LPS.
authors.affiliation: Department of Psychiatry and Neurosciences, Hiroshima University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kugaya
authors.forename: A
authors.initials: A
authors.lastname: Kagaya
authors.forename: H
authors.initials: H
authors.lastname: Zensho
authors.forename: T
authors.initials: T
authors.lastname: Oyamada
authors.forename: Y
authors.initials: Y
authors.lastname: Tawara
authors.forename: M
authors.initials: M
authors.lastname: Inagaki
authors.forename: Y
authors.initials: Y
authors.lastname: Uchitomi
authors.forename: S
authors.initials: S
authors.lastname: Yamawaki
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D019332:Endothelin-1 / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein; D005910:Glioma; D007375:Interleukin-1 / Q000494:pharmacology*; D007529:Isoflavones / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D019332:Endothelin-1; D004791:Enzyme Inhibitors; D007375:Interleukin-1; D007529:Isoflavones; D008070:Lipopolysaccharides; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D002118:Calcium