title: Fatty acid alteration and the lateral diffusion of lipids in the plasma membrane of keratinocytes.
abstract: The fluorescent probe diI was used to study the lateral mobility of lipids in in vitro strains of living adult human keratinocytes grown in four different media. One medium was essential fatty acid deficient (EFAD) and low in calcium ion, a medium known to yield cells that proliferate rapidly and contain lipid with extremely low levels of essential fatty acids. Two other media were supplemented with essential fatty acids (FAS), media that are known to result in cells that grow more slowly and have normalized fatty acid proportions. A fourth medium consisted of 1 microM all-trans-retinoic acid added to the fatty acid-supplemented medium (FAS-RA), a medium known to produce cells that are highly proliferative, with a growth rate greater than that of the FAS strains and similar to that of the EFAD strains. The keratinocytes grown in these four media were studied using the fluorescence recovery after photobleaching (FRAP) technique to determine the lateral diffusion rate of diI in the plasma membranes. Our results showed a positive correlation between growth rate and diffusion coefficient (D): the diffusion coefficient of diI was higher in the EFAD or FAS-RA cells than in the FAS cells. The measurement of D among the FAS cells fell into two groups. One group was similar to the single group seen in the EFAD cells, but the other group was composed of much lower D values. The other FRAP parameters (mobile fraction and bleach depth) were larger in the "slow" group than in the "fast" group. This trend of negative correlation between these parameters and D was also found within the fast group. These results are interpreted in terms of possible changes in membrane structure or morphology that might be indirectly associated with the fatty acid alterations, including the possible presence of areas in senescing keratinocytes where plasma membranes collapse to form an interacting system of lipid bilayers.
authors.affiliation: Biophysics Research Division, University of Michigan, Ann Arbor 48109-1055, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Fulbright
authors.forename: D
authors.initials: D
authors.lastname: Axelrod
authors.forename: W R
authors.initials: WR
authors.lastname: Dunham
authors.forename: C L
authors.initials: CL
authors.lastname: Marcelo
mesh_terms: D000345:Affinity Labels; D002232:Carbocyanines; D002455:Cell Division; D002462:Cell Membrane / Q000502:physiology*; D003470:Culture Media; D004058:Diffusion; D005227:Fatty Acids / Q000378:metabolism*; D005456:Fluorescent Dyes; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*; D050356:Lipid Metabolism; D008560:Membrane Fluidity / Q000502:physiology*; D012867:Skin / Q000166:cytology
chemical_list: D000345:Affinity Labels; D002232:Carbocyanines; D003470:Culture Media; D005227:Fatty Acids; D005456:Fluorescent Dyes