title: Pathogenesis of infection induced by an adenovirus isolated from a goat.
abstract: OBJECTIVE
  To determine the pathogenic potential of an adenovirus isolated from a goat.
  
  
  METHODS
  14 colostrum-deprived, isolation-reared goat kids approximately 3 weeks old.
  
  
  METHODS
  Kids were inoculated with either cell culture fluid containing adenovirus (n = 10) or uninfected cell culture fluid (n = 4): 2 ml transtracheally and 1 ml/nostril. Clinical signs of disease and rectal temperature were recorded daily; nasal secretion and fecal specimens were collected daily. Control kids were necropsied, 2/d, on postinoculation days (PID) 5 and 10. Virus-inoculated kids were necropsied on PID 3, 5, 7, 10, and 28. After necropsy, lung, liver, kidney, and brain specimens were aseptically collected for virus isolation attempts. Tracheal fluid was collected on sterile cotton swabs. Turbinate, trachea, lung, mediastinal lymph node, liver, kidney, duodenum, jejunum, ileum, mesenteric lymph node, colon, and brain specimens were collected for histologic evaluation.
  
  
  RESULTS
  Kids developed mild-to-moderate clinical respiratory tract infection. Virus was recovered consistently from nasal secretion and sporadically from fecal specimens. Grossly, there were multiple areas of atelectasis and hyperemia, principally in the cranioventral portion of the lungs. Microscopically, there was detachment and sloughing of foci of epithelial cells of the terminal bronchioles and alveoli. In kids necropsied late in the disease, these changes were accompanied by hyperplasia of type-II epithelial cells. Viral inclusions were not an obvious feature, but a few cells contained probable inclusions.
  
  
  CONCLUSIONS
  The caprine adenovirus reported here is capable of inducing respiratory tract disease and lesions in the lungs of young kids.
authors.affiliation: Respiratory and Neurologic Disease Research Unit, United States Department of Agriculture, Ames, IA 50010, USA.
authors.forename: H D
authors.initials: HD
authors.lastname: Lehmkuhl
authors.forename: R C
authors.initials: RC
authors.lastname: Cutlip
authors.forename: J T
authors.initials: JT
authors.lastname: Meehan
authors.forename: B M
authors.initials: BM
authors.lastname: DeBey
mesh_terms: D000256:Adenoviridae / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D000257:Adenoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001831:Body Temperature / Q000502:physiology; D001980:Bronchi / Q000737:chemistry / Q000473:pathology / Q000821:virology; D003126:Colostrum / Q000502:physiology; D015511:Goat Diseases / Q000276:immunology / Q000503:physiopathology / Q000821:virology*; D006041:Goats; D008168:Lung / Q000737:chemistry / Q000473:pathology / Q000821:virology; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000473:pathology / Q000821:virology; D012141:Respiratory Tract Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology
chemical_list: D000914:Antibodies, Viral