title: Cell cultures and associated retroviruses in multiple sclerosis. Collaborative Research Group on MS.
abstract: Retroviral particles associated with reverse transcriptase (RT) activity in cell-cultures from MS patients have been reported by different groups. Cell-cultures have been used for the study and characterization of the corresponding retroviral genome which we have shown is related to ERV9 in the pol region. Previously unpublished details of a study with monocyte cultures are presented together with observations on leptomeningeal and choroid-plexus cultures. The generation of self-transformed cultures after inhibition of interferon, followed by the loss of retroviral expression and recurrent apoptosis, is analyzed. Retroviral particles with RT-activity are produced in monocyte cultures with an apparent correlation with MS disease activity. However, though leptomeningeal and choroid plexus cells from MS can be passaged for a limited period, their evolution in vitro is not compatible with stable retroviral expression. These culture limitations greatly hampered progress on the elucidation of the retroviral genome sequence.
authors.affiliation: BioMérieux S.A., UMR 103 CNRS-bioMérieux, Lyon, France.
authors.forename: H
authors.initials: H
authors.lastname: Perron
authors.forename: R
authors.initials: R
authors.lastname: Firouzi
authors.forename: P
authors.initials: P
authors.lastname: Tuke
authors.forename: J A
authors.initials: JA
authors.lastname: Garson
authors.forename: M
authors.initials: M
authors.lastname: Michel
authors.forename: F
authors.initials: F
authors.lastname: Beseme
authors.forename: F
authors.initials: F
authors.lastname: Bedin
authors.forename: F
authors.initials: F
authors.lastname: Mallet
authors.forename: E
authors.initials: E
authors.lastname: Marcel
authors.forename: J M
authors.initials: JM
authors.lastname: Seigneurin
authors.forename: B
authors.initials: B
authors.lastname: Mandrand
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002455:Cell Division / Q000235:genetics; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002472:Cell Transformation, Viral / Q000235:genetics; D002478:Cells, Cultured / Q000821:virology; D002831:Choroid Plexus / Q000821:virology; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D015751:Genes, pol / Q000235:genetics; D006801:Humans; D008297:Male; D008578:Meninges / Q000821:virology; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009000:Monocytes / Q000821:virology*; D009103:Multiple Sclerosis / Q000821:virology*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D012192:Retroviridae Infections / Q000235:genetics / Q000821:virology*
chemical_list: D012194:RNA-Directed DNA Polymerase