Development of a direct PCR assay for detection of the diphtheria toxin gene.
PCR has proved to be a reliable tool for the detection of the diphtheria toxin gene, tox, and its use has allowed for the rapid differentiation between toxigenic and nontoxigenic strains. In this study, this PCR was further developed, evaluated, and standardized to detect this gene directly from clinical specimens. Optimal conditions for collection, transport, and storage of the clinical specimens and isolation and purification of DNA from the clinical specimens were defined. With two sets of primers that detect the A and B subunits of the diphtheria toxin gene, sensitivity levels of 50 and 500 CFU/PCR mixture, respectively, were achieved. This PCR was evaluated with 162 clinical samples collected from patients with diphtheria and other upper respiratory tract infections, as well as from healthy individuals.
Nakao
H
H
Division of Bacterial and Mycotic Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Popovic
T
T
D003353:Corynebacterium diphtheriae; D004167:Diphtheria Toxin / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods*
D004167:Diphtheria Toxin