Effects of estrogen on cell growth and fibroblast growth factor receptor induction in MtT/Se cells.
The effects of estradiol (E2) on cell growth and the expression of fibroblast growth factor receptor (FGFR) were investigated in a cultured rat anterior pituitary cell line (MtT/Se) established from an E2-induced mammotropic pituitary tumor. E2 stimulated cell growth 2-3 fold as compared to the control. Basic fibroblast growth factor (bFGF) stimulated cell growth in the presence of E2 (10(-9) M) but not in the absence of E2. Tamoxifen as an antiestrogen agent inhibited the proliferation of MtT/Se cells which had been treated with E2 or E2 and bFGF. Numbers of FGFR in E2-stimulated MtT/Se cells significantly exceeded those in cells not treated with E2. These observations indicate that E2 plays an important role in the regulation of FGFR induction in the anterior pituitary cells.
Takahashi
H
H
Department of Biochemistry, School of Medicine, Nihon University, Tokyo, Japan.
Nakagawa
S
S
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004958:Estradiol / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D015151:Immunoblotting; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis*; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D017468:Receptors, Fibroblast Growth Factor; D013629:Tamoxifen; D016222:Fibroblast Growth Factor 2; D004958:Estradiol