[Changes in the hematopoietic system and in the concentrations of blood-regulatory cytokines after acute irradiation and a combined radiation-thermal lesion].
Acute radiation and combined radiation-thermal injuries (CRTIs) were modelled in the experiments on CBA x C57B16 mice. Changes in the concentrations of endogenic and lipopolysaccharide-induced TNF-alpha in the blood serum were measured in 3 to 24 h after gamma-irradiation at a dose of 7 Gy and a combined radiation-thermal injury. The status of mature and reserve pools of the bone marrow granulocytes was determined from the reaction of leukocytes mobilization to blood in response to the administration of interleukin-1 beta (IL-1 beta) in 48 h after Irradiation and CRTI. The contents of IL-3 and IL-6 in the blood serum, the level of cytokine secretion by the bone marrow cells were determined in vitro in the peak phases of acute radiation and combined injuries. No significant changes in the reaction of the bone marrow cells and in the level of pancytopenia were revealed after acute radiation and CRTIs, which could help in understanding an increase in the death rates after CRTIs. Also, quantitative changes in the TNF and IL-6 concentrations in the blood serum were estimated along with those in the level of secretion of IL-3 by the bone marrow cells of mice exposed to radiation and CRTIs.
Ul'ianova
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LP
Budagov
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RS
D000208:Acute Disease; D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D002056:Burns / Q000097:blood* / Q000401:mortality; D016207:Cytokines / Q000097:blood / Q000528:radiation effects*; D005720:Gamma Rays; D006413:Hematopoietic System / Q000528:radiation effects*; D007958:Leukocyte Count / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011833:Radiation Injuries, Experimental / Q000097:blood* / Q000401:mortality; D013997:Time Factors; D014916:Whole-Body Irradiation / Q000009:adverse effects
D016207:Cytokines