Cloning and molecular analysis of the plasma membrane Ca(2+)-ATPase gene in Paramecium tetraurelia.
We have determined the DNA sequence of the gene encoding the protein of the plasma membrane Ca(2+)-ATPase in Paramecium tetraurelia. The predicted amino acid sequence of the plasma membrane Ca(2+)-ATPase shows homology to conserved regions of known plasma membrane Ca(2+)-ATPases and contains the known binding sites for ATP (FITC), acylphosphate formation, and calmodulin, as well as the "hinge" region: all characteristics common to plasma membrane Ca(2+)-ATPases. The deduced molecular weight for this sequence is 131 kDa. The elucidation of this gene will assist in the studies of the mechanisms by which this excitable cell removes calcium entering through voltage gated calcium channels and the pump functions in chemosensory signal transduction.
Elwess
N L
NL
Department of Biology, University of Vermont, Burtington 05405, USA.
Van Houten
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017125:Genes, Protozoan; D008565:Membrane Proteins / Q000032:analysis; D008969:Molecular Sequence Data; D016805:Paramecium tetraurelia / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000032:analysis; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D008565:Membrane Proteins; D015800:Protozoan Proteins; D000252:Calcium-Transporting ATPases