Human IgGFc binding protein (FcgammaBP) in colonic epithelial cells exhibits mucin-like structure.
Cloning a cDNA for human IgGFc binding protein (FcgammaBP) from human colonic epithelial cells reveals an mRNA and coding region of 17 and 16.2 kilobases, respectively. The predicted amino acid sequence contains 12 occurrences of a 400-amino acid cysteine-rich unit resembling that found in mucin. A motif (CGLCGN) in FcgammaBP is conserved in MUC2 and prepro-von Willebrand factor. The N-terminal 450-amino acid sequences are necessary and sufficient to confer IgG Fc binding activity. FcgammaBP mRNA is expressed only in placenta and colonic epithelial cells. These results suggest that FcgammaBP may play an important role in immune protection and inflammation in the intestines of primates.
Harada
N
N
Tokyo Institute for Immunopharmacology, Inc., and Chugai Pharmaceutical Co. Ltd., Tokyo 171, Japan.
Iijima
S
S
Kobayashi
K
K
Yoshida
T
T
Brown
W R
WR
Hibi
T
T
Oshima
A
A
Morikawa
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002352:Carrier Proteins / Q000737:chemistry*; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003106:Colon / Q000737:chemistry* / Q000166:cytology; D006224:Cricetinae; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry*; D016376:Oligonucleotides, Antisense; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014841:von Willebrand Factor / Q000737:chemistry
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; C106607:FCGBP protein, human; D008565:Membrane Proteins; D009077:Mucins; D016376:Oligonucleotides, Antisense; D011994:Recombinant Proteins; D014841:von Willebrand Factor