title: [A new method of ex vivo whole blood perfusion of isolated mammalian organs, exemplified by the kidney of swine].
abstract: A new method for the ex vivo perfusion of organs from large mammals is described. Gas exchange and dialysis are carried out simultaneously with a low-flux polysulfon dialysis module. The dialysate (e.g. Tyrode solution) is aerated with a mixture of oxygen and carbon dioxide to ensure gas exchange with the blood. Dialysis is carried out in a closed thermostatically controlled system. Monitoring of ultrafiltration is maintained by continuously weighing the blood reservoir and adjusting an afferent and efferent blood pump. Initial results obtained with isolated pig kidneys demonstrate the suitability of the new method for use as a model for the replacement of animal experiments. Theoretically, clinical application in the area of in vivo regional organ perfusion may also be possible.
authors.affiliation: Universitätsklinikum Rudolf Virchow der Humboldt Universität zu Berlin.
authors.forename: H
authors.initials: H
authors.lastname: von Baeyer
authors.forename: K
authors.initials: K
authors.lastname: Stahl
authors.forename: M
authors.initials: M
authors.lastname: Häusler
authors.forename: M
authors.initials: M
authors.lastname: Meissler
authors.forename: V
authors.initials: V
authors.lastname: Unger
authors.forename: J
authors.initials: J
authors.lastname: Frank
authors.forename: C
authors.initials: C
authors.lastname: Grosse-Siestrup
authors.forename: G
authors.initials: G
authors.lastname: Kaczmarczyk
authors.forename: K
authors.initials: K
authors.lastname: Affeld
authors.forename: H J
authors.initials: HJ
authors.lastname: Flaig
authors.forename: B
authors.initials: B
authors.lastname: Steinbach
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D010478:Chemotherapy, Cancer, Regional Perfusion / Q000295:instrumentation; D015314:Dialysis Solutions; D004867:Equipment Design; D007668:Kidney / Q000098:blood supply*; D010100:Oxygen / Q000097:blood; D010477:Perfusion / Q000295:instrumentation*; D006435:Renal Dialysis / Q000295:instrumentation*; D013552:Swine
chemical_list: D015314:Dialysis Solutions; D002245:Carbon Dioxide; D010100:Oxygen