Electrospray mass spectrometry of alpha and beta chains of selected hemoglobins and their TNBA and TNB conjugates.
The molecular weights of alpha and beta hemoglobin chains from 15 different vertebrate animal sources and 2 common human variants were determined by electrospray mass spectrometry and compared to the calculated masses based on published amino acid sequences. Conjugates were prepared for 14 of the globins using 2 traditional colorimetric derivatizing reagents, 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and trinitrobenzenesulfonic acid (TNBS), and the mass of each conjugate was determined by mass spectrometry.
Adamczyk
M
M
Department of Chemistry (D9NM), Abbott Laboratories, Abbott Park, Illinois 60064. USA. adamczykm@apmac.abbott.com
Gebler
J C
JC
D000818:Animals; D002415:Cats; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D008970:Molecular Weight; D009579:Nitrobenzoates / Q000737:chemistry*; D010215:Papio; D013438:Sulfhydryl Compounds; D014301:Trinitrobenzenes / Q000737:chemistry*
D006454:Hemoglobins; D009579:Nitrobenzoates; D013438:Sulfhydryl Compounds; D014301:Trinitrobenzenes; C011136:thionitrobenzoic acid