title: A dendritic-cell-derived C-C chemokine that preferentially attracts naive T cells.
abstract: Dendritic cells form a system of highly efficient antigen-presenting cells. After capturing antigen in the periphery, they migrate to lymphoid organs where they present the antigen to T cells. Their seemingly unique ability to interact with and sensitize naive T cells gives dendritic cells a central role in the initiation of immune responses and allows them to be used in therapeutic strategies against cancer, viral infection and other diseases. How they interact preferentially with naive rather than activated T lymphocytes is still poorly understood. Chemokines direct the transport of white blood cells in immune surveillance. Here we report the identification and characterization of a C-C chemokine (DC-CK1) that is specifically expressed by human dendritic cells at high levels. Tissue distribution analysis demonstrates that dendritic cells present in germinal centres and T-cell areas of secondary lymphoid organs express this chemokine. We show that DC-CK1, in contrast to RANTES, MIP-1alpha and interleukin-8, preferentially attracts naive T cells (CD45RA+). The specific expression of DC-CK1 by dendritic cells at the site of initiation of an immune response, combined with its chemotactic activity for naive T cells, suggests that DC-CK1 has an important rule in the induction of immune responses.
authors.affiliation: Department of Tumour Immunology, University Hospital Nijmegen, The Netherlands. g.adema@dent.kun.nl
authors.forename: G J
authors.initials: GJ
authors.lastname: Adema
authors.forename: F
authors.initials: F
authors.lastname: Hartgers
authors.forename: R
authors.initials: R
authors.lastname: Verstraten
authors.forename: E
authors.initials: E
authors.lastname: de Vries
authors.forename: G
authors.initials: G
authors.lastname: Marland
authors.forename: S
authors.initials: S
authors.lastname: Menon
authors.forename: J
authors.initials: J
authors.lastname: Foster
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: P
authors.initials: P
authors.lastname: Nooyen
authors.forename: T
authors.initials: T
authors.lastname: McClanahan
authors.forename: K B
authors.initials: KB
authors.lastname: Bacon
authors.forename: C G
authors.initials: CG
authors.lastname: Figdor
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D018925:Chemokines / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D019742:Chemokines, CC; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D018076:DNA, Complementary; D003713:Dendritic Cells / Q000737:chemistry* / Q000276:immunology; D018858:Germinal Center / Q000166:cytology / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D017403:In Situ Hybridization; D015847:Interleukin-4 / Q000502:physiology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D017386:Sequence Homology, Amino Acid; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013997:Time Factors
chemical_list: C111792:CCL18 protein, human; D018925:Chemokines; D019742:Chemokines, CC; D018076:DNA, Complementary; D012333:RNA, Messenger; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D017493:Leukocyte Common Antigens