title: Evidence for in vitro and in vivo autocatalytic processing of the primary translation product of beet necrotic yellow vein virus RNA 1 by a papain-like proteinase.
abstract: Beet necrotic yellow vein virus RNA 1 contains a single long ORF corresponding to the theoretical translation product of 237 kDa which contains the information necessary for replication of the viral genome. This ORF contains a putative papain-like proteinase domain which has been localized, on the basis of sequence alignments, between the helicase and polymerase domains. Here we show that the RNA 1 primary translation product can be cleaved autocatalytically in vitro into two species of 150 kDa and 66 kDa, the latter of which probably contains the entire polymerase domain. A 66 kDa protein was detected immunologically in infected C. quinoa protoplasts using an antiserum specific for the C-terminal region of the RNA 1 primary translation product, confirming that processing also occurs in vivo.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
authors.forename: A
authors.initials: A
authors.lastname: Hehn
authors.forename: C
authors.initials: C
authors.lastname: Fritsch
authors.forename: K E
authors.initials: KE
authors.lastname: Richards
authors.forename: H
authors.initials: H
authors.lastname: Guilley
authors.forename: G
authors.initials: G
authors.lastname: Jonard
mesh_terms: D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010206:Papain / Q000494:pharmacology; D010942:Plant Viruses / Q000235:genetics*; D014176:Protein Biosynthesis; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D010206:Papain