Construction of specific erythromycin resistance mutations in the temperate lactococcal bacteriophage TP901-1 and their use in studies of phage biology.
A method for the construction and isolation of specifically designed mutations of the temperate lactococcal phage TP901-1 has been developed. Two different erm-labeled mutants were isolated. One was shown to be defective in lysogenization and excision. The other, showing normal lysogenization, was used for host range studies.
Koch
B
B
Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Christiansen
B
B
Evison
T
T
Vogensen
F K
FK
Hammer
K
K
D001435:Bacteriophages / Q000187:drug effects* / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology*; D013294:Lactococcus lactis / Q000821:virology*; D008242:Lysogeny / Q000235:genetics; D009154:Mutation; D015183:Restriction Mapping; D014161:Transduction, Genetic
D004279:DNA, Viral; D004917:Erythromycin