Lymphocyte proliferative responses of goats vaccinated with Brucella melitensis 16M or a delta purE201 strain.
The response to a Brucella melitensis purEK deletion mutant, delta purE201 (referred to as strain 201), was compared with the response to its parental strain, 16M, in juvenile goats. Proliferative responses to gamma-irradiated bacteria were detected earlier in strain 201-infected goats. Lymphocytes from strain 16M- or 201-infected goats proliferated in response to one-dimensional polyacrylamide gel electrophoresis-separated proteins of similar mass isolated from strain 16M or Brucella abortus RB51. Data from this study suggest that some antigens stimulating cell-mediated responses are conserved among Brucella species, as 201- and 16M-infected goats recognized similar proteins expressed by RB51 and 16M.
Olsen
S C
SC
National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010, USA. SOlsen@nadc.ars.usda.com
Cheville
N F
NF
Stevens
M G
MG
Houng
H H
HH
Drazek
E S
ES
Hadfield
T L
TL
Warren
R L
RL
Hoover
D L
DL
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D017347:Brucella melitensis / Q000276:immunology* / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006041:Goats / Q000276:immunology*; D008213:Lymphocyte Activation; D014611:Vaccination / Q000662:veterinary
D001428:Bacterial Vaccines