Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP).
The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples. The assay is reliable and reproducible in routine analyses and can be used to estimate the processivity of telomerase activity.
Kim
N W
NW
Geron Corporation, Menlo Park, CA 94025, USA. nkim@geron.com
Wu
F
F
D017931:DNA Primers; D019281:Dimerization; D005188:False Negative Reactions; D006801:Humans; D009369:Neoplasms / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011786:Quality Control; D012091:Repetitive Sequences, Nucleic Acid; D019098:Telomerase / Q000032:analysis* / Q000378:metabolism*; D016615:Telomere
D017931:DNA Primers; D019098:Telomerase