Alterations of mRNA levels of alpha 1-adrenoceptor subtypes with maturation and ageing in different rat blood vessels.
1. Alterations of mRNA levels of alpha 1-adrenoceptor subtypes during maturation and ageing were determined by reverse transcription-polymerase chain reaction (RT-PCR) in aortae and renal, pulmonary and mesenteric arteries isolated from 3, 12 and 24-month-old rats. 2. The steady state levels for alpha 1A-, alpha 1B- and alpha 1D-adrenoceptors in aorta declined with maturation and ageing. In renal artery there was a decrease in mRNA for the alpha 1B-adrenoceptor in aged rats. However, in mesenteric and pulmonary arteries there were no changes in mRNA levels for the three subtypes of alpha 1-adrenoceptors as a result of maturation and ageing. 3. The results suggest that expression of alpha 1-adrenoceptors is changed heterogeneously in different blood vessels during maturation and ageing in rats.
Xu
K M
KM
Institute of Vascular Medicine, Third Hospital, Beijing Medical University, China.
Tang
F
F
Han
C
C
D000375:Aging / Q000378:metabolism* / Q000502:physiology*; D000818:Animals; D001013:Aorta, Thoracic / Q000254:growth & development / Q000378:metabolism; D001158:Arteries / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D008638:Mesenteric Arteries / Q000254:growth & development / Q000378:metabolism; D011651:Pulmonary Artery / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018340:Receptors, Adrenergic, alpha-1 / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D012077:Renal Artery / Q000254:growth & development / Q000378:metabolism
D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1