title: Long-term alterations in growth factor mRNA expression following seizures.
abstract: Although alterations in growth factor mRNA occur during neuronal insults, little is known about the long-term effects of neuronal insults on growth factor expression. We have examined the effects of prolonged post-ictal times on the expression of Brain-derivered nerve factor (BDNF) and Neurotrophin 3 (NT3) following Kainic acid (KA)-induced seizures. In situ hybridization was performed on male Sprague-Dawley rats sacrificed 1-2 weeks following intracranial ventricular KA injections. BDNF mRNA increased bilaterally 1 and 2 weeks after injections, whereas NT3 mRNA decreased contralaterally 1 week and bilaterally 2 weeks post-injection. These observations provide evidence that alterations in growth factor mRNA expression occur even after prolonged post-ictal recovery suggesting a possible role for growth factors in recovery and continued maintenance of surviving neurons within limbic seizure foci.
authors.affiliation: Department of Molecular Neuroscience, Mayo Clinic Rochester, MN 55905, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Garcia
authors.forename: V B
authors.initials: VB
authors.lastname: Garcia
authors.forename: P J
authors.initials: PJ
authors.lastname: Isackson
authors.forename: A J
authors.initials: AJ
authors.lastname: Windebank
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis; D013006:Growth Hormone / Q000235:genetics*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009414:Nerve Growth Factors / Q000096:biosynthesis; D020933:Neurotrophin 3; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000378:metabolism*; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D012333:RNA, Messenger; D013006:Growth Hormone