title: Adjuvant effect of respiratory irritation on pulmonary allergic sensitization: time and site dependency.
abstract: It has been suggested that airway irritation, by acting as an adjuvant, as well as producing damage, may be an important factor related to asthma. The present study examined the window of time following acute upper and lower airway irritant exposure to determine the period of increased risk of immunological sensitization. Brown Norway rats were exposed to 87 ppm NO2 or 1000 ppm NH3 for 1 hr. A 30-min ovalbumin (OVA) exposure of 18.14 microg/liter air was given at various times based upon the time course of irritant associated inflammatory response (either immediately prior to or 1 or 7 days after the irritant exposure). OVA-only, NO2-only or NH3-only controls, and saline controls were also studied. Weekly booster exposures of OVA (or saline) were given. Circulating OVA-specific IgE, IgA, and IgG levels were quantified periodically during the 6 weeks of the study. Bronchoalveolar lavage (BAL) was also performed to examine the inflammatory response to allergic and irritant challenge. Significant increases in OVA-specific IgE, IgG, and IgA antibody titers were seen in rats given the sensitizing OVA exposure within 1 day of the NO2, but not NH3 exposures. Enhancement of cellular infiltrate in BAL was noted in groups given the sensitizing OVA exposure within 1 day of the NO2 or NH3. It is concluded that the inflammatory and immunological response to antigen exposure can be modified by the site of respiratory tract irritation and the relative times of irritant and antigen exposure.
authors.affiliation: Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA.
authors.forename: P D
authors.initials: PD
authors.lastname: Siegel
authors.forename: N H
authors.initials: NH
authors.lastname: Al-Humadi
authors.forename: E R
authors.initials: ER
authors.lastname: Nelson
authors.forename: D M
authors.initials: DM
authors.lastname: Lewis
authors.forename: A F
authors.initials: AF
authors.lastname: Hubbs
mesh_terms: D000276:Adjuvants, Immunologic / Q000633:toxicity*; D000641:Ammonia / Q000633:toxicity*; D000818:Animals; D001249:Asthma / Q000139:chemically induced* / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D007136:Immunoglobulins / Q000097:blood / Q000187:drug effects; D007509:Irritants / Q000633:toxicity*; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008297:Male; D009585:Nitrogen Dioxide / Q000633:toxicity*; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D007136:Immunoglobulins; D007509:Irritants; D000641:Ammonia; D010047:Ovalbumin; D009585:Nitrogen Dioxide