title: Molecular genetic analysis of HLA class II alleles in Japanese patients with melanoma.
abstract: Distribution of HLA-DQA, -DQB and -DPB alleles in ninety-six Japanese patients with melanoma was analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and the association between clinical parameters and the presence of certain HLA class II alleles investigated. The frequency of HLA-DQB1*0302 was increased, while those of DQA1*0101(04), -DQA1*0401 and DRB1*0802 were decreased in melanoma patients compared with controls. Moreover, the frequency of HLA-DQA1*0103 in patients with acral lentiginous melanoma was increased compared with controls. However, none of these HLA class II alleles showed significant positive or negative associations after correction of the P value. In addition, there was no correlation between these antigens and clinical parameters. These results indicate that HLA class II alleles may not contribute to a strong susceptibility to melanoma in the Japanese.
authors.affiliation: Department of Dermatology, Kumamoto University School of Medicine, Japan. toshiro@kaiju.medic.kumamoto-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Kageshita
authors.forename: T
authors.initials: T
authors.lastname: Naruse
authors.forename: S
authors.initials: S
authors.lastname: Hirai
authors.forename: T
authors.initials: T
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Horikoshi
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
authors.forename: K
authors.initials: K
authors.lastname: Tamaki
authors.forename: K
authors.initials: K
authors.lastname: Hayashibe
authors.forename: M
authors.initials: M
authors.lastname: Ichihashi
authors.forename: J
authors.initials: J
authors.lastname: Nakayama
authors.forename: Y
authors.initials: Y
authors.lastname: Hori
authors.forename: A
authors.initials: A
authors.lastname: Ozawa
authors.forename: M
authors.initials: M
authors.lastname: Miyahara
authors.forename: M
authors.initials: M
authors.lastname: Ohkido
authors.forename: H
authors.initials: H
authors.lastname: Inoko
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D007564:Japan; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged
chemical_list: D000949:Histocompatibility Antigens Class II