Further biochemical and biophysical characterisation of scyllin, Scylla serrata hemolymph lectin.
The amino acid and carbohydrate analysis of scyllin, a low molecular weight lectin purified from Scylla serrata (edible crab) haemolymph reveal that scyllin is rich in acidic and neutral amino acids and contains high amount of mannose. UV absorption of scyllin is perturbed by DMSO at 272 nm showing the presence of tryptophan molecule in scyllin exposed and accessible to the solvent. The oxidation of tryptophan molecule by N-bromosuccinimide results in loss of haemagglutinating activity of lectin. The study of thermodynamic parameters of scyllin-glycoproteins interaction suggests that ceruloplasmin is the most potent inhibitors of scyllin of all the glycoproteins studied.
Chattopadhyay
T
T
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta, India.
Chatterjee
B
B
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001974:Bromosuccinimide / Q000737:chemistry; D002241:Carbohydrates / Q000032:analysis; D002570:Ceruloplasmin / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006384:Hemagglutination; D006458:Hemolymph / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000737:chemistry*; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014364:Tryptophan / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D037102:Lectins; D014364:Tryptophan; D002570:Ceruloplasmin; D001974:Bromosuccinimide; D008358:Mannose; D004121:Dimethyl Sulfoxide