Integration of HTLV-1 provirus into mouse transforming growth factor-alpha gene.
Human T cell leukemia virus type 1 (HTLV-1) provirus integration was investigated in mice inoculated with MT-2 cells, a HTLV-1 producing human T-cell line. From spleen DNA derived from a MT-2 cell-injected mouse, we cloned a HTLV-1 integration site by ligation-mediated PCR. The nucleotide sequence showed that HTLV-1 provirus was integrated into an intron of the mouse transforming growth factor-alpha gene in the reverse orientation. This result provides for the first time molecular evidence that mice can be infected with HTLV-1.
Fang
J
J
Department of Biochemistry and Molecular Oncology, University of Tsukuba, Ibaraki, Japan.
Kushida
S
S
Feng
R
R
Tanaka
M
M
Kikukawa
H
H
Kawamura
T
T
Uchida
K
K
Miwa
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics*; D016211:Transforming Growth Factor alpha / Q000235:genetics*; D016662:Virus Integration / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D016211:Transforming Growth Factor alpha