The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC.
The yeast splicing factor BBP (branchpoint bridging protein) interacts directly with pre-mRNA at or very near the highly conserved branchpoint sequence UACUAAC within the commitment complex. We also show that the recombinant protein recognizes the UACUAAC sequence. Therefore, BBP is also an acronym for branchpoint binding protein. The mammalian splicing factor SF1 is a BBP ortholog (mBBP) and an E complex component, and also has branchpoint sequence specificity. The relative conservation of this region in yeast and mammals correlates well with the RNA-binding differences between BBP and mBBP, suggesting that BBP contributes to branchpoint sequence definition in both systems.
Berglund
J A
JA
Howard Hughes Medical Institute, Department of Biology and Biochemistry, Brandeis University, Waltham, Massachusetts 02254, USA.
Chua
K
K
Abovich
N
N
Reed
R
R
Rosbash
M
M
D001665:Binding Sites / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae; D017421:Sequence Analysis
D005656:Fungal Proteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins