title: Amperometric immunosensors based on rigid conducting immunocomposites.
abstract: Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgC1). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay.
authors.affiliation: Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Santandreu
authors.forename: F
authors.initials: F
authors.lastname: Céspedes
authors.forename: S
authors.initials: S
authors.lastname: Alegret
authors.forename: E
authors.initials: E
authors.lastname: Martínez-Fàbregas
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001672:Biocompatible Materials; D015374:Biosensing Techniques; D003188:Composite Resins / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D008689:Methacrylates / Q000737:chemistry; D010755:Organophosphates / Q000378:metabolism; D011817:Rabbits; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003188:Composite Resins; D004852:Epoxy Compounds; D007074:Immunoglobulin G; D008689:Methacrylates; D010755:Organophosphates; D018030:Silver Compounds; D012834:Silver; C074782:phenylphosphate; D006108:Graphite; C037548:silver chloride