title: Rotavirus infections in young Nicaraguan children.
abstract: BACKGROUND
  Rotavirus is an important cause of dehydrating diarrhea in young children throughout the world. Knowledge about frequency of reinfections, development of immunity to the virus and the possible protective effect of breast milk is important, in particular in relation to possible strategies for immunization.
  
  
  METHODS
  A prospective study of rotavirus infections in a cohort of 235 infants followed from birth until 2 years of age was performed in León, Nicaragua. Fecal and serum specimens were collected at specified times, and stools were also obtained during episodes of diarrhea. Fecal specimens were screened by rotavirus antigen detection and serum and colostral specimens were analyzed by isotype-specific rotavirus antibody enzyme-linked immunosorbent assay.
  
  
  RESULTS
  As judged by anti-rotavirus IgA antibody seroconversion and/or demonstration of rotavirus antigen in fecal specimens, > 50% of the babies had evidence of past rotavirus infection by the age of 2 months. The total incidence of rotavirus infections, including many reinfections, was 0.7 infection/child-year, of which only 17% were associated with diarrhea. The time from birth to the first demonstration of rotavirus in stool samples correlated significantly with the concentration of anti-rotavirus IgA antibodies in colostrum. There was also a tendency toward a relationship between long duration of breast-feeding and asymptomatic infection.
  
  
  CONCLUSIONS
  Rotavirus infections are acquired very early in infants in León, Nicaragua, and reinfections are common. Most infections are asymptomatic. Breast milk appears to confer partial protection against rotavirus infection, probably mediated by specific IgA antibodies. To be effective rotavirus vaccination would probably have to be given at a very early age to infants in developing countries.
authors.affiliation: Department of Microbiology, National University (UNAN) León, Nicaragua.
authors.forename: F
authors.initials: F
authors.lastname: Espinoza
authors.forename: M
authors.initials: M
authors.lastname: Paniagua
authors.forename: H
authors.initials: H
authors.lastname: Hallander
authors.forename: L
authors.initials: L
authors.lastname: Svensson
authors.forename: O
authors.initials: O
authors.lastname: Strannegård
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011446:Prospective Studies; D012008:Recurrence; D012401:Rotavirus / Q000145:classification; D012400:Rotavirus Infections / Q000276:immunology* / Q000517:prevention & control; D014611:Vaccination
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G