title: Molecular properties of the erythromycin resistance plasmid pPV141 from Staphylococcus chromogenes.
abstract: The 2.3-kb erythromycin resistance (EmR) plasmid pPV141 of Staphylococcus chromogenes 3688 was isolated and characterized. Nucleotide sequence analysis identified ORF1 and ORF2 separated by a 445-bp spacing, encoding a 158-residue replication protein (Rep141) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respectively. Structural analysis and Southern hybridization showed that the rep and ermM genes in pPV141 shared homology with other known EmR plasmids. Based on sequence analysis, pPV141 was classified as a unique member of the pSN2 family of EmR plasmids.
authors.affiliation: U.S. Department of Agriculture, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Somkuti
authors.forename: D K
authors.initials: DK
authors.lastname: Solaiman
authors.forename: D H
authors.initials: DH
authors.lastname: Steinberg
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004265:DNA Helicases / Q000235:genetics; D004261:DNA Replication; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins; D004352:Drug Resistance, Microbial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology*; D005798:Genes, Bacterial; D008780:Methyltransferases / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011815:R Factors / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013210:Staphylococcus / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D013816:Thermodynamics; D015534:Trans-Activators / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004917:Erythromycin; D008780:Methyltransferases; C047346:16S rRNA (adenine(1518)-N(6)-adenine(1519)-N(6))-dimethyltransferase; D004265:DNA Helicases