Effects of 5,7-dihydroxytryptamine lesions of the prefrontal cortex on consumption of sucrose-ethanol solutions: relationship to prefrontal monoamines.
Thirty adult male Wistar rats received 8 micrograms bilaterally of 5,7-dihydroxytryptamine into the medial prefrontal cortex (mPFC). Rats were then trained, via a sucrose, fading paradigm, to consume increasing concentrations of alcohol. After death, dopamine (DA), norepinephrine (NE), serotonin (5-HT), and their metabolites were measured in the mPFC, nucleus accumbens (NA), and raphe nucleus. The lesioned group demonstrated a reduction in 5-hydroxyindoleacetic acid (5-HIAA), DA, and NE in the mPFC (p < 0.05), and a trend toward reduction of 5-HT in the NA. In comparison with controls, lesioned animals consumed less of all solutions containing sucrose and alcohol. On regression analyses, monoamines in the mPFC (i.e., 5-HIAA, dihydrophenylacetic acid and NE) predicted consumption of the 5% ethanol solution (p = 0.009), 10% ethanol solution (p = 0.0006), and the 5% sucrose solutions (p = 0.0006), but not the 20% sucrose solutions. In each case, monoamine levels were positively correlated with consumption. No relationships were seen between monoamine levels in the NA and raphe, and in consummatory behavior.
Deckel
A W
AW
Neuropsychology Service, University of Connecticut Medical School, Farmington, USA.
Shoemaker
W J
WJ
Arky
L
L
D015116:5,7-Dihydroxytryptamine; D000428:Alcohol Drinking / Q000503:physiopathology*; D000437:Alcoholism / Q000503:physiopathology*; D000818:Animals; D001931:Brain Mapping; D019422:Dietary Sucrose / Q000008:administration & dosage*; D004298:Dopamine / Q000502:physiology*; D008297:Male; D009042:Motivation; D009638:Norepinephrine / Q000502:physiology*; D009714:Nucleus Accumbens / Q000502:physiology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000503:physiopathology*; D011903:Raphe Nuclei / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000502:physiology*; D013649:Taste / Q000187:drug effects / Q000502:physiology
D019422:Dietary Sucrose; D015116:5,7-Dihydroxytryptamine; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine