title: Mitochondrial acyl carrier protein is involved in lipoic acid synthesis in Saccharomyces cerevisiae.
abstract: The yeast gene, ACP1, encoding the mitochondrial acyl carrier protein, was deleted by gene replacement. The resulting acp1-deficient mutants had only 5-10% of the wild-type lipoic acid content remaining, and exhibited a respiratory-deficient phenotype. Upon meiosis, the lipoate deficiency co-segregated with the acp1 deletion. The role of ACP1 in long-chain fatty acid synthesis was studied in fast and fas2 null mutants completely lacking cytoplasmic fatty acid synthase. When grown on odd-chain (13:0 and 15:0) fatty acids, these cells showed less than 1% of C-16 and C-18 acids in their total lipids. Mitochondrial ACP is therefore suggested to be involved with the biosynthesis of octanoate, a precursor to lipoic acid.
authors.affiliation: Department of Biology, University of California at San Diego, La Jolla 92093, USA.
authors.forename: S
authors.initials: S
authors.lastname: Brody
authors.forename: C
authors.initials: C
authors.lastname: Oh
authors.forename: U
authors.initials: U
authors.lastname: Hoja
authors.forename: E
authors.initials: E
authors.lastname: Schweizer
mesh_terms: D000213:Acyl Carrier Protein / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D064429:Fatty Acid Synthases / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D005800:Genes, Fungal; D008540:Meiosis; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D008063:Thioctic Acid / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D000213:Acyl Carrier Protein; D005231:Fatty Acids, Unsaturated; D005656:Fungal Proteins; D008063:Thioctic Acid; D064429:Fatty Acid Synthases