Nucleotide sequencing of a part of the 5'-noncoding region of echovirus type 9 and rapid virus detection during the acute phase of aseptic meningitis.
A part of the 5'-noncoding region of echovirus type 9 isolates was sequenced, and an attempt was made for rapid virus detection in clinical samples obtained from 22 subjects hospitalized with aseptic meningitis. The sequence identity of 440-bp products amplified from the region by RT-PCR was 87.7% between the standard echovirus type 9(Hill strain) and the isolates. Specific IgM antibodies to Hill strain were positive in 45.5% by immunofluorescent antibody staining of virus-infected cells. A high detection rate of PCR products was observed in cerebrospinal fluids (CSFs; 54.5%) at admission, and in peripheral mononuclear cells (PMCs; 72.7%) at the end of hospitalization. Viral genomes were detectable for 2 days in serum samples, and for 6 days in PMC samples after onset of disease. When specific IgM antibody titers were less than 1:40, the amplification rate of viral genome from serum samples was 50.0%. These results indicate that the combination of specific IgM determination and viral genome amplification from CSFs will be a rapid and reliable method for early diagnosis.
Kim
G R
GR
Department of Microbiology, Catholic University, Medical College, Seoul, Korea.
Lee
J S
JS
Jung
Y T
YT
Chung
Y J
YJ
Rhyu
M G
MG
D000208:Acute Disease; D000914:Antibodies, Viral / Q000134:cerebrospinal fluid; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D018076:DNA, Complementary; D004459:Echovirus 9 / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007223:Infant; D008297:Male; D008582:Meningitis, Aseptic / Q000821:virology*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D000914:Antibodies, Viral; D018076:DNA, Complementary; D007074:Immunoglobulin G; D007075:Immunoglobulin M