An efficient and reliable multiplex PCR-SSCP mutation analysis test applied to the human E-cadherin gene.
The invasion suppressor gene E-CADHERIN (CDH1) is downregulated in a large variety of human carcinomas. Up to now, mutational analysis of the CDH1 gene has been described for 325 tumors derived from only four different tissue types. A simple but sensitive mutation detection assay is needed to screen many more tumor types, possibly bearing E-cadherin inactivating mutations. For that purpose, we developed a multiplex PCR-SSCP analysis for all 16 CDH1 exons. Ease of experimentation was combined with reliable sensitivity. Indeed, the present multiplex analysis reduces the number of manipulations to 50%, while the mutation detection turned out to be highly efficient and sensitive.
Berx
G
G
Department of Molecular Biology, University of Ghent, Belgium.
Nollet
F
F
Strumane
K
K
van Roy
F
F
D001483:Base Sequence; D015820:Cadherins / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods* / Q000706:statistics & numerical data; D017931:DNA Primers / Q000235:genetics; D015536:Down-Regulation; D005091:Exons; D006801:Humans; D007438:Introns; D009154:Mutation; D009369:Neoplasms / Q000235:genetics; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D018807:Polymorphism, Single-Stranded Conformational; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015820:Cadherins; D017931:DNA Primers