Cytomegalovirus infection in newborns and their family members: polymerase chain reaction analysis of isolates.
Polymerase chain reaction (PCR) analysis with primers for the pp65, a-sequence, glycoprotein B, and major immediate early genes of human cytomegalovirus (CMV) was used to study five congenitally-infected infants and their CMV-infected family members. Family members excreting CMV included three mothers and two siblings. The PCR results indicated that the CMV strain excreted by each infant was indistinguishable from that excreted by the corresponding family member. By contrast, the molecular profiles of the CMV strains were distinct between families, indicating that the PCR algorithm described in this study is a useful method for analyzing CMV strains.
Souza
I E
IE
Dept. of Pediatrics, University of Iowa College of Medicine, Iowa City, USA.
Gregg
A
A
Pfab
D
D
Dawson
J D
JD
Benson
P
P
O'Neill
M E
ME
Murph
J R
JR
Petheram
S J
SJ
Bale
J F
JF
D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000175:diagnosis / Q000635:transmission / Q000821:virology; D018562:Disease Transmission, Infectious; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D007223:Infant; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis* / Q000821:virology; D011446:Prospective Studies; D014759:Viral Envelope Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics; D017201:Virus Shedding / Q000235:genetics
D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D010750:Phosphoproteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; C040495:glycoprotein B, Simplexvirus; C044582:immediate-early proteins, cytomegalovirus