Effect of crosslinked poly(1-vinyl-2-pyrrolidinone) gels on cell growth in static cell cultures.
Poly(1-vinyl-2-pyrrolidinone) (PVP) and copolymers of 1-vinyl-2-pyrrolidinone are insoluble in water when crosslinked but they can absorb very large amounts of water to become syringe-injectable hydrogels. Such gels have been investigated recently as potential substitutes for the vitreous humour in the eye. In this study, during the cytotoxic evaluation by sulforhodamine B colorimetric assay of variously crosslinked PVP gels, it was found that many of them showed protective/growth promoting effects on 3T3 mouse fibroblasts in static cultures, a phenomenon encountered previously only with aqueous solutions of a limited number of natural or synthetic polymers. Particularly, the gels crosslinked with diethylene glycol dimethacrylate (DEGDMA) induced a significant enhancement of cell proliferation, especially in serum-free cultures. No correlation between this effect and the essential gel properties (chemical composition, viscoelasticity and equilibrium water content) could be established. The study demonstrated that crosslinked PVP hydrogels showed a serum-like growth promoting effect on an anchorage-dependent cell line, which may be due to physical protection, inability of the insoluble gels to penetrate cell membranes, and their ability to mimic the extracellular matrix.
Hong
Y
Y
Lions Eye Institute, Department of Biomaterials and Polymer Research, Nedlands, Western Australia, Australia.
Chirila
T V
TV
Fitton
J H
JH
Ziegelaar
B W
BW
Constable
I J
IJ
D016475:3T3 Cells / Q000187:drug effects*; D000042:Absorption; D000818:Animals; D001082:Aqueous Humor; D001187:Artificial Organs; D002455:Cell Division / Q000187:drug effects*; D003124:Colorimetry; D016895:Culture Media, Serum-Free; D004548:Elasticity; D008422:Materials Testing; D008689:Methacrylates; D051379:Mice; D011145:Polyvinyls / Q000737:chemistry* / Q000494:pharmacology*; D011760:Pyrrolidinones / Q000737:chemistry* / Q000494:pharmacology*; D012235:Rhodamines; D014783:Viscosity
D016895:Culture Media, Serum-Free; D008689:Methacrylates; D011145:Polyvinyls; D011760:Pyrrolidinones; D012235:Rhodamines; C099680:poly(1-vinyl-2-pyrrolidinone); C022027:lissamine rhodamine B; C004919:ethylene dimethacrylate