Decreased short-term production of tumor necrosis factor-alpha and interleukin-1beta in human immunodeficiency virus-seropositive subjects.
Tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) were measured by ELISA in 4.5-h, lipopolysaccharide-stimulated whole blood cultures of 347 human immunodeficiency virus type 1-infected patients and 107 healthy seronegative controls. The production of TNF-alpha was decreased in both AIDS and non-AIDS patients, whereas the production of IL-1beta was decreased in AIDS patients only. The production of TNF-alpha and IL-1beta was positively affected by the concentrations of CD14+ monocytes and CD8+ lymphocytes; however, in patients, the concentration of CD4+ lymphocytes and the presence of AIDS had a negative effect on cytokine production as determined by multiple linear regression analysis. It is concluded that low whole blood cytokine production is mainly caused by low numbers of cells, but a functional defect may also exist.
Ullum
H
H
Department of Infectious Diseases and Institute for Inflammation Research, Rhima Centre, Rigshospitalet, Copenhagen, Denmark.
Victor
J
J
Katzenstein
T L
TL
Gerstoft
J
J
Gøtzsche
P C
PC
Bendtzen
K
K
Skinhøj
P
P
Pedersen
B K
BK
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000276:immunology; D018791:CD4 Lymphocyte Count; D018414:CD8-Positive T-Lymphocytes; D006679:HIV Seropositivity / Q000097:blood* / Q000276:immunology; D015497:HIV-1; D006801:Humans; D007375:Interleukin-1 / Q000097:blood*; D018950:Lipopolysaccharide Receptors / Q000097:blood; D016131:Lymphocyte Subsets; D009000:Monocytes / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D019562:Viral Load
D007375:Interleukin-1; D018950:Lipopolysaccharide Receptors; D014409:Tumor Necrosis Factor-alpha