title: Cloning and functional expression of a novel glucuronyltransferase involved in the biosynthesis of the carbohydrate epitope HNK-1.
abstract: The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules and also on some glycolipids in the nervous system over a wide range of species from insect to mammal. The HNK-1 epitope is involved in cell-cell and/or cell-substrate interaction and recognition during the development of the nervous system. In this study, we isolated a novel glucuronyltransferase from rat brain, which is a key enzyme of the biosynthesis of the HNK-1 epitope on glycoproteins. Based on the partial amino acid sequences, we isolated cDNA encoding the glucuronyltransferase. The primary structure deduced from the cDNA sequence predicted a type II transmembrane protein with 347 amino acids and had no detectable similarity with any other proteins of known functions, including glucuronyltransferases of the liver and olfactory epithelium. Expression of a soluble recombinant form of the enzyme in COS-1 cells produced an active glucuronyltransferase. The selective expression of the glucuronyltransferase gene in the nervous system was consistent with the almost exclusive localization of the HNK-1 epitope in the nervous system. Transfection of the glucuronyltransferase cDNA into COS-1 cells induced not only expression of the HNK-1 epitope on the cell surface but also marked morphological changes of the cells, suggesting that the HNK-1 epitope associates with the cell-substratum interaction.
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Terayama
authors.forename: S
authors.initials: S
authors.lastname: Oka
authors.forename: T
authors.initials: T
authors.lastname: Seiki
authors.forename: Y
authors.initials: Y
authors.lastname: Miki
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Kozutsumi
authors.forename: K
authors.initials: K
authors.lastname: Takio
authors.forename: T
authors.initials: T
authors.lastname: Kawasaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D018959:CD57 Antigens / Q000032:analysis / Q000096:biosynthesis*; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D007313:Insecta; D008322:Mammals; D008969:Molecular Sequence Data; D009420:Nervous System / Q000201:enzymology; D016133:Polymerase Chain Reaction; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: D018959:CD57 Antigens; D018076:DNA, Complementary; D011994:Recombinant Proteins; D014453:Glucuronosyltransferase