title: Preliminary investigations of an amperometric oligosaccharide dehydrogenase-based electrode for the detection of glucose and some other low molecular weight saccharides.
abstract: Biosensors for the determination of sugars were constructed using oligosaccharide dehydrogenase (ODH) and soluble phenazine methosulfate (PMS) or an osmium-based three-dimensional redox hydrogel. In the latter case the enzyme and poly(1-vinylimidazole) complexed with osmium (4,4'-dimethylbpy)2Cl were cross-linked with poly(ethylene glycol) diglycidyl ether. Both electrode configurations showed similar sensitivities for glucose in the range between 8 and 21 muAmM-1 cm-2. The responses for 10 mono and oligosaccharides were studied. There was no response for fructose. In the concentration range 0.1-20 mM the relative sensitivities were determined for arabinose (96%), xylose (3%), mannose (50%), galactose (11%), glucose (100%), maltose (24%), lactose (12%), cellobiose (34%) and maltotriose (10%).
authors.affiliation: Enzyme Chemistry Laboratory, Institute of Biochemistry, Vilnius, Lithuania.
authors.forename: T
authors.initials: T
authors.lastname: Ruzgas
authors.forename: E
authors.initials: E
authors.lastname: Csöregi
authors.forename: I
authors.initials: I
authors.lastname: Katakis
authors.forename: G
authors.initials: G
authors.lastname: Kenausis
authors.forename: L
authors.initials: L
authors.lastname: Gorton
mesh_terms: D015374:Biosensing Techniques; D002138:Calibration; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D008773:Methylphenazonium Methosulfate / Q000737:chemistry; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D009992:Osmium / Q000737:chemistry; D011108:Polymers; D012680:Sensitivity and Specificity
chemical_list: D009844:Oligosaccharides; D011108:Polymers; D008773:Methylphenazonium Methosulfate; D009992:Osmium; D002237:Carbohydrate Dehydrogenases; C107242:oligosaccharide dehydrogenase; D005947:Glucose