Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation.
Tip of herpesvirus saimiri associates with Lck and down-regulates Lck-mediated activation. We identified a novel cellular Tip-associated protein (Tap) by a yeast two-hybrid screen. Tap associated with Tip following transient expression in COS-1 cells and stable expression in human Jurkat-T cells. Expression of Tip and Tap in Jurkat-T cells induced dramatic cell aggregation. Aggregation was likely caused by the up-regulated surface expression of adhesion molecules including integrin alpha, L-selectin, ICAM-3, and H-CAM. Furthermore, NF-kappaB transcriptional factor of aggregated cells had approximately 40-fold higher activity than that of parental cells. Thus, Tap is likely to be an important cellular mediator of Tip function in T cell transformation by herpesvirus saimiri.
Yoon
D W
DW
Department of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772-9102, USA.
Lee
H
H
Seol
W
W
DeMaria
M
M
Rosenzweig
M
M
Jung
J U
JU
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002449:Cell Aggregation / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D029543:Nucleocytoplasmic Transport Proteins; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins; D013601:T-Lymphocytes / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D016328:NF-kappa B; C122111:NXF1 protein, human; D029543:Nucleocytoplasmic Transport Proteins; D010750:Phosphoproteins; D011506:Proteins; D016601:RNA-Binding Proteins; D014764:Viral Proteins; C092384:tyrosine kinase interacting protein, Saimiriine herpesvirus 2