Incidence of chromosome 1 disomy in human sperm estimated by the primed in situ (PRINS) labeling technique.
The primed in situ (PRINS) labeling technique was used to determine the rate of disomy of chromosomes 1 and 16 in sperm of two normal subjects. Two different but specific primers (alpha-satellite and satellite II) for chromosome 1 were used in parallel experiments to test the efficiency of PRINS labeling in sperm nuclei. A minimum of 10,000 sperm nuclei per chromosome primer was analyzed, leading to a total number of 41,651 scored spermatozoa. Similar rates of chromosome 1 disomy (mean values, 0.18% and 0.20%) were found in both donors when the alpha-satellite and satellite II primers were used, demonstrating the reliability of PRINS labeling on sperm nuclei. For chromosome 16, the disomy rate among the two donors ranged from 0.20% to 0.24%. This study confirms that PRINS provides a rapid and efficient method for in situ chromosomal screening of sperm nuclei.
Pellestor
F
F
CNRS ERS 155, Montpellier, France.
Girardet
A
A
Andréo
B
B
Lefort
G
G
Charlieu
J P
JP
D000328:Adult; D002878:Chromosomes, Human, Pair 1; D017931:DNA Primers; D004276:DNA, Satellite; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism*
D017931:DNA Primers; D004276:DNA, Satellite