title: Frequent loss of heterozygosity on chromosome arm 18q in squamous cell carcinomas. Identification of 2 regions of loss--18q11.1-q12.3 and 18q21.1-q23.
abstract: OBJECTIVE
  To determine the frequency and regions of loss on chromosome arm 18q in uncultured head and neck squamous cell carcinomas.
  
  
  METHODS
  Polymerase chain reaction amplification of DNA extracted from 18 tumor specimens (1 patient had 2 tumors) and blood samples from 17 patients with head and neck squamous cell carcinoma was performed using primers flanking 16 microsatellite repeat polymorphisms spanning most of chromosome 18q. DNA was extracted only from specimens with greater than 70% tumor nuclei.
  
  
  METHODS
  Research university.
  
  
  METHODS
  Seventeen individuals with newly diagnosed head and neck cancer.
  
  
  METHODS
  Loss of heterozygosity (LOH).
  
  
  RESULTS
  There was LOH at more than 1 locus in 52% (9/ 17) of the tumors; 3 tumors had LOH at all informative markers. Four had loss at only 1 locus, raising the total with loss to 12 (75%) of 16. Loss of 18q11.1-q12.3 in 4 tumors without distal loss defines a proximal region of loss. Loss of heterozygosity affecting 18q21.1 in 1 tumor, without proximal loss and LOH for 18q21.1, 18q22, or 18q23 in 9 (52%) of 17 tumors defines a distal region of loss.
  
  
  CONCLUSIONS
  Loss of heterozygosity on chromosome arm 18q is not an artifact of in vitro culture. The finding of 18q LOH in 50% to 70% tumors makes 18q an important region for study. Regions 18q11.1-q12.3 and 18q21.1-q23 are common regions of loss, indicating that there may be more than one 18q tumor suppressor gene involved in the genesis and progression of head and neck squamous cell carcinomas.
authors.affiliation: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Jones
authors.forename: J R
authors.initials: JR
authors.lastname: Raval
authors.forename: T F
authors.initials: TF
authors.lastname: Beals
authors.forename: M J
authors.initials: MJ
authors.lastname: Worsham
authors.forename: D L
authors.initials: DL
authors.lastname: Van Dyke
authors.forename: R M
authors.initials: RM
authors.lastname: Esclamado
authors.forename: G T
authors.initials: GT
authors.lastname: Wolf
authors.forename: C R
authors.initials: CR
authors.lastname: Bradford
authors.forename: T
authors.initials: T
authors.lastname: Miller
authors.forename: T E
authors.initials: TE
authors.lastname: Carey
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002872:Chromosome Deletion; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
chemical_list: D004273:DNA, Neoplasm