Conversion of biocytin labelled cells and structures for the confocal laser-scanning method.
The method for converting biocytin preparations of brain sections fills a gap in the application of confocal laser-scanning microscopy. Both neuronal and non-neuronal structures are converted. The background remains free of staining. The protocol can be applied to old and already existing biocytin-(diaminobenzidine)-nickel preparations which are then made accessible to evaluation with the laser-scanning microscope by the substitution of nickel with silver-gold. Sodium thiosulphate is used to remove the unbound silver. The reflection image of the laser-scanning microscopy provides more information than the transmission image.
Hilbig
H
H
Institute of Anatomy, Leipzig University, Department of Medicine, Germany.
Müller
A
A
D000818:Animals; D001921:Brain / Q000166:cytology*; D008239:Lysine / Q000031:analogs & derivatives*; D018613:Microscopy, Confocal / Q000379:methods*; D009474:Neurons / Q000502:physiology*; D051381:Rats
C013411:biocytin; D008239:Lysine