[The positive effect of pretreatment with serotonin and gangliosides on the development of early mouse embryos after cryopreservation].
We have demonstrated that viability of preimplantation mouse embryos F1 (C57B1/6 x CBA) after cryoconservation at the stage of four blastomeres improves after pretreatment with serotonin (5 HT, 5 microM) or total gangliosides of bovine brain gangliosides (TG, 3 microM) added to the cultivation medium. After thawing, 64% of embryos preincubated with total brain gangliosides and 49% of embryos preincubated with serotonin developed to the stage of blastocyst during the cultivation in vitro; in the control, no more than 25% of embryos reaches this stage, and all these embryos were abnormal. Possible mechanisms of protective action of these compounds is discussed. We conclude that mouse embryos subjected to freezing-thawing procedure can be used to examine the role of serotonin and gangliosides in the regulatory processes of mammalian preimplantation development.
Sakharova
N Iu
NIu
Markova
L N
LN
Lepikhov
K A
KA
Zvezdina
N D
ND
Buznikov
G A
GA
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D001757:Blastomeres / Q000187:drug effects; D002417:Cattle; D015925:Cryopreservation; D046508:Culture Techniques; D005328:Fetal Viability; D005732:Gangliosides / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D012701:Serotonin / Q000494:pharmacology*; D013268:Stimulation, Chemical
D005732:Gangliosides; D012701:Serotonin