title: Incidence of chromosome 1 disomy in human sperm estimated by the primed in situ (PRINS) labeling technique.
abstract: The primed in situ (PRINS) labeling technique was used to determine the rate of disomy of chromosomes 1 and 16 in sperm of two normal subjects. Two different but specific primers (alpha-satellite and satellite II) for chromosome 1 were used in parallel experiments to test the efficiency of PRINS labeling in sperm nuclei. A minimum of 10,000 sperm nuclei per chromosome primer was analyzed, leading to a total number of 41,651 scored spermatozoa. Similar rates of chromosome 1 disomy (mean values, 0.18% and 0.20%) were found in both donors when the alpha-satellite and satellite II primers were used, demonstrating the reliability of PRINS labeling on sperm nuclei. For chromosome 16, the disomy rate among the two donors ranged from 0.20% to 0.24%. This study confirms that PRINS provides a rapid and efficient method for in situ chromosomal screening of sperm nuclei.
authors.affiliation: CNRS ERS 155, Montpellier, France.
authors.forename: F
authors.initials: F
authors.lastname: Pellestor
authors.forename: A
authors.initials: A
authors.lastname: Girardet
authors.forename: B
authors.initials: B
authors.lastname: Andréo
authors.forename: G
authors.initials: G
authors.lastname: Lefort
authors.forename: J P
authors.initials: JP
authors.lastname: Charlieu
mesh_terms: D000328:Adult; D002878:Chromosomes, Human, Pair 1; D017931:DNA Primers; D004276:DNA, Satellite; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D004276:DNA, Satellite