In vitro transcriptional activation of p21 promoter by p53.
The tumor suppressor p53 can control cell growth by inducing cell cycle arrest. This is effected, at least in part, by transcriptional activation of p21 gene, a cell cycle inhibitor. Here in vitro transcription assay for p21 has been established. The assay recapitulates activation of p21 promoter mediated by p53 in vitro. Thus this in vitro assay will allow to study how transcriptional regulation of p21 can be linked to cell cycle controls during development and neoplastic transformation processes.
Kim
T K
TK
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
D000818:Animals; D002453:Cell Cycle; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D016158:Genes, p53; D051379:Mice; D009154:Mutation; D011401:Promoter Regions, Genetic; D051381:Rats; D015533:Transcriptional Activation; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism*
C493957:Cdkn1a protein, mouse; C493958:Cdkn1a protein, rat; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D016159:Tumor Suppressor Protein p53; D004247:DNA