A comparison of two-dimensional electrophoresis data with phenotypical traits in Arabidopsis leads to the identification of a mutant (cri1) that accumulates cytokinins.
Total proteins extracted from developmental mutants of Arabidopsis thaliana (L.) Heyhn. and from wild-type plants cultivated in the presence of various hormones were analyzed by two-dimensional (2-D) gel electrophoresis. Computer analysis of 2-D gels followed by a statistical treatment of data allowed us to build a phenogram that describes the biochemical distances between the different genotypes. Analysis of the 2-D electrophoresis data allowed us to discriminate mutants in agreement with phenotypical and physiological traits. This biochemical analysis helped us to develop a working hypothesis which led us to show that one developmental mutant (cri1) overaccumulates cytokinins.
Santoni
V
V
Biochimie et Physiologie Végétales, INRA/ENSA-M/CNRS, Montpellier, France. santoniv@ensam.inra.fr
Delarue
M
M
Caboche
M
M
Bellini
C
C
D017360:Arabidopsis / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D009154:Mutation; D010641:Phenotype; D010940:Plant Proteins / Q000032:analysis*
D010940:Plant Proteins