title: Whole joint embedding in polymethylmethacrylate: a method for preparing intact musculoskeletal organ systems for histomorphology and 3-D reconstruction.
abstract: Large and medium size undecalcified joints were embedded in methylmethacrylate resin. Sections of 600 microns prepared from polymethylmethacrylate blocks show minimal distortion and are suitable for surface staining and three-dimensional reconstruction. The 5 microns sections prepared from these slabs retain good cytological detail. This method permits the examination of musculoskeletal organ systems at both macroscopic and microscopic levels.
authors.affiliation: McCaig Centre for Joint Injury and Arthritis Research, University of Calgary, Alberta, Canada.
authors.forename: J R
authors.initials: JR
authors.lastname: Matyas
authors.forename: M G
authors.initials: MG
authors.lastname: Anton
authors.forename: C B
authors.initials: CB
authors.lastname: Frank
mesh_terms: D000818:Animals; D002415:Cats; D007719:Knee Joint / Q000033:anatomy & histology; D008768:Methylmethacrylates; D008867:Microtomy; D009141:Musculoskeletal System / Q000033:anatomy & histology*; D016613:Plastic Embedding / Q000379:methods*; D011817:Rabbits; D013194:Staining and Labeling
chemical_list: D008768:Methylmethacrylates