title: New assay for glycated lipoproteins by high-performance liquid chromatography.
abstract: We developed an automated analytic system for glycated lipoprotein using high-performance liquid chromatography (HPLC) with an affinity boronate column and a gel permeation column. This system can measure glycated lipoprotein (glycated LDL and glycated HDL) in a small (5 microliters) sample of serum in a short time (40 min/sample). Recovery with this system was 92.1%. Therefore a large number of samples can be measured in clinical use. The system should contribute to an elucidation of the role of glycated lipoprotein in atherosclerosis.
authors.affiliation: Third Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Yui
authors.forename: N
authors.initials: N
authors.lastname: Tomie
authors.forename: T
authors.initials: T
authors.lastname: Baba
authors.forename: M
authors.initials: M
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Makita
authors.forename: F
authors.initials: F
authors.lastname: Numano
authors.forename: S
authors.initials: S
authors.lastname: Nakatani
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood*
chemical_list: D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL