title: Dietary modulation of fatty acid composition of mast cell phospholipids does not affect histamine release induced by compound 48/80.
abstract: OBJECTIVE
  In the present study we determined the extent to which the degranulation process in mast cells was related to the fatty acid composition of membrane phospholipids.
  
  
  METHODS
  Peritoneal mast cells were isolated from Wistar rats (3 groups of 18 animals each), fed for 6 weeks diets which differed in their fatty acid compositions: (i) genuine salmon oil, abundant in (n-3) fatty acids, (ii) sunflower seed oil, rich in (n-6) fatty acids, particularly linoleic acid, and (iii) hydrogenated coconut oil, rich in saturated fatty acids.
  
  
  METHODS
  Mast cells (10(6)/ml) were stimulated with various concentrations of the mast cell-degranulating agent, compound 48/80 (0.1-10 micrograms/ml). The extent of mast cell degranulation was quantified by determination of histamine in the supernatants using HPLC techniques.
  
  
  RESULTS
  No differences in compound 48/80-induced histamine release between the three dietary groups for any of the concentrations of compound 48/80 tested were found. Analysis of variance followed by Tukey's method for multiple comparisons was used to evaluate the effect of changes in the dietary fat type.
  
  
  CONCLUSIONS
  These findings strongly suggest that in contrast to the formation of eicosanoids, the process of mast cell degranulation by a receptor-independent pathway is not controlled by the fatty acid composition of membrane phospholipids.
authors.affiliation: Department of Internal Medicine, Maastricht University, The Netherlands.
authors.forename: W
authors.initials: W
authors.lastname: Engels
authors.forename: C M
authors.initials: CM
authors.lastname: Van Haaster
authors.forename: P J
authors.initials: PJ
authors.lastname: Lemmens
authors.forename: G J
authors.initials: GJ
authors.lastname: Van der Vusse
authors.forename: G
authors.initials: G
authors.lastname: Hornstra
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects* / Q000502:physiology; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D000074263:Coconut Oil; D004041:Dietary Fats / Q000008:administration & dosage / Q000032:analysis; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage* / Q000032:analysis; D005227:Fatty Acids / Q000008:administration & dosage / Q000032:analysis*; D005395:Fish Oils / Q000008:administration & dosage / Q000737:chemistry; D006636:Histamine Release / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008407:Mast Cells / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D008563:Membrane Lipids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D000074242:Sunflower Oil; D003189:p-Methoxy-N-methylphenethylamine / Q000633:toxicity*
chemical_list: D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005395:Fish Oils; D008563:Membrane Lipids; D010743:Phospholipids; D010938:Plant Oils; D000074242:Sunflower Oil; D003189:p-Methoxy-N-methylphenethylamine; C030341:salmon oil; D000074263:Coconut Oil