Some recent developments in the preparation of novel recognition systems: a recognition site for the selective catalysis of an aldol condensation using molecular imprinting and specific affinity motifs for alpha-chymotrypsin using a phage display peptide library.
Molecular imprinting and phage display library technologies are rapidly being accepted as useful techniques for the generation of ligand-selective recognition motifs. The use of molecular imprinting to produce a novel type II aldolase mimic selective for the cobalt(II)-mediated aldol condensation of benzophenone and acetaldehyde is reported here. Furthermore, peptide motifs have been identified which are acting as 'affinity ligands' selective for the recognition of the enzyme alpha-chymotrypsin using phage display techniques.
Nicholls
I A
IA
Department of Pure and Applied Biochemistry, University of Lund, Sweden.
Matsui
J
J
Krook
M
M
Mosbach
K
K
D000079:Acetaldehyde / Q000737:chemistry; D001565:Benzoates / Q000737:chemistry; D002384:Catalysis; D047188:Chalcones; D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism*; D003035:Cobalt / Q000737:chemistry; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry / Q000378:metabolism*; D018716:Molecular Mimicry; D019151:Peptide Library; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D011725:Pyridines / Q000737:chemistry; D013343:Styrenes / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry
D001565:Benzoates; D047188:Chalcones; D019151:Peptide Library; D011108:Polymers; D011725:Pyridines; D013343:Styrenes; D014753:Vinyl Compounds; D003035:Cobalt; C061481:dibenzoylmethane; D002918:Chymotrypsin; D005634:Fructose-Bisphosphate Aldolase; D000079:Acetaldehyde; C004985:divinyl benzene