Purification of a regulatory subunit of type II cAMP-dependent protein kinase from Drosophila heads.
The cytosolic extract from Drosophila heads was separated using anion-exchange column chromatography. Two types of cAMP-dependent protein kinase (PKA), type I and type II, were detected, and type II PKA was found to be a major isozyme. The regulatory subunit of type II PKA (RII) was purified, and only one isoform was observed. The purified protein had an apparent molecular mass of 51 kDa on SDS gel electrophoresis. Partial amino acid sequences of the protein were almost identical with the RII alpha subunit of human. Since PKA has been implicated to be especially important for learning and memory in Drosophila, the RII subunit may play an essential role in the regulation of neuronal activity in the brain of Drosophila, and possibly in human.
Inoue
H
H
Department of Molecular Neurobiology, School of Human Sciences, Waseda University, Tokorozawa, Japan. hiroko@human.waseda.ac.jp
Yoshioka
T
T
D000345:Affinity Labels; D000818:Animals; D002852:Chromatography, Ion Exchange; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism; D054754:Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D004330:Drosophila / Q000201:enzymology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006257:Head; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry; D017421:Sequence Analysis; D012697:Serine Endopeptidases / Q000378:metabolism
D000345:Affinity Labels; D054754:Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit; C517819:PRKAR2A protein, human; D010446:Peptide Fragments; D000242:Cyclic AMP; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase