Determination of albuterol in plasma after aerosol inhalation by gas chromatography-mass spectrometry with selected-ion monitoring.
Albuterol is a beta2-adrenergic agonist commonly used as a bronchodilator for the treatment of patients with asthma. We have developed an assay to determine plasma levels as low as 50 pg/ml of albuterol by gas chromatography-mass spectrometry (GC-MS). This assay utilizes isotopically labeled albuterol ([13C]albuterol) as an internal standard. In this assay albuterol and the internal standard are recovered from 1 ml of plasma using solid-phase extraction. The samples are then derivatized to trimethylsilyl ethers using N,O-bis(trimethylsilyl)trifluoro-acetamide with 1% trimethylchlorosilane. The samples are then analyzed by GC-MS with selected-ion monitoring (SIM) for the ions m/z 369.15 and 370.15. The method has been validated for a concentration range of 50-10000 pg/ml in plasma.
Logsdon
T W
TW
Department of Biopharmaceutical Sciences, University of Arkansas for Medical Sciences and John L. McClellan Veterans Administration Hospital, Little Rock 72205, USA.
Zhou
X
X
Breen
P
P
Anderson
P
P
Gann
L
L
Hiller
C
C
Compadre
C M
CM
D000280:Administration, Inhalation; D000420:Albuterol / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D001993:Bronchodilator Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007477:Ions; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001993:Bronchodilator Agents; D007477:Ions; D000420:Albuterol