Whole joint embedding in polymethylmethacrylate: a method for preparing intact musculoskeletal organ systems for histomorphology and 3-D reconstruction.
Large and medium size undecalcified joints were embedded in methylmethacrylate resin. Sections of 600 microns prepared from polymethylmethacrylate blocks show minimal distortion and are suitable for surface staining and three-dimensional reconstruction. The 5 microns sections prepared from these slabs retain good cytological detail. This method permits the examination of musculoskeletal organ systems at both macroscopic and microscopic levels.
Matyas
J R
JR
McCaig Centre for Joint Injury and Arthritis Research, University of Calgary, Alberta, Canada.
Anton
M G
MG
Frank
C B
CB
D000818:Animals; D002415:Cats; D007719:Knee Joint / Q000033:anatomy & histology; D008768:Methylmethacrylates; D008867:Microtomy; D009141:Musculoskeletal System / Q000033:anatomy & histology*; D016613:Plastic Embedding / Q000379:methods*; D011817:Rabbits; D013194:Staining and Labeling
D008768:Methylmethacrylates