title: Singlet oxygen mediates the activation of JNK by UVA radiation in human skin fibroblasts.
abstract: Ultraviolet A (UVA: 320-400 nm) radiation activates c-Jun-N-terminal kinase (JNK 2) in human skin fibroblasts. Exposure of cells to UVA (300 kJ/m2) led to a 5-fold induction of JNK-activity which was significantly increased in the presence of D2O, an enhancer of the lifetime of singlet oxygen. Sodium azide, a quencher of singlet oxygen, abolished the activation of JNK. A hydroxyl radical scavenger, mannitol, had no effect. Furthermore, photochemically produced singlet oxygen (Rose Bengal plus white light) was found to induce JNK activity. This was enhanced by D2O and inhibited by azide. Thus, singlet oxygen activates and mediates the UVA-induced activation of JNK.
authors.affiliation: Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, Germany.
authors.forename: L O
authors.initials: LO
authors.lastname: Klotz
authors.forename: K
authors.initials: K
authors.lastname: Briviba
authors.forename: H
authors.initials: H
authors.lastname: Sies
mesh_terms: D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis* / Q000378:metabolism; D002478:Cells, Cultured; D004790:Enzyme Induction / Q000528:radiation effects; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases; D007700:Kinetics; D008027:Light; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism*; D012395:Rose Bengal / Q000494:pharmacology; D026082:Singlet Oxygen; D012867:Skin / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D016166:Free Radical Scavengers; D026082:Singlet Oxygen; D012395:Rose Bengal; D008353:Mannitol; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D010100:Oxygen