title: In vitro metabolism of the new insecticide flupyrazofos by rat liver microsomes.
abstract: 1. The in vitro metabolism of the new insecticide flupyrazofos was studied using rat liver microsomes. Two metabolites were produced and identified as O,O-diethyl O-(1-phenyl-3-trifluoromethyl-5-pyrazoyl) phosphoric acid ester (flupyrazofos oxon) and 1-phenyl-3-trifluoromethyl-5-hydroxypyrazole (PTMHP) based on UV and mass spectral analysis. 2. Cytochrome P450 oxidatively converted flupyrazofos to flupyrazofos oxon, a major metabolite and phenobarbital-induced microsomes increased this desulphuration by 8-fold. 3. Flupyrazofos oxon was converted to PTMHP with a half-life of 47.8 min by chemical hydrolysis and this conversion also proceeded non-enzymatically under our microsomal incubation conditions.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Jeong
authors.forename: K
authors.initials: K
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.forename: B H
authors.initials: BH
authors.lastname: Kang
authors.forename: J K
authors.initials: JK
authors.lastname: Roh
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D015394:Molecular Structure; D010278:Parathion / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007306:Insecticides; D007527:Isoenzymes; D010278:Parathion; D003577:Cytochrome P-450 Enzyme System