Biochemical characterization of the mouse muscle-specific enolase: developmental changes in electrophoretic variants and selective binding to other proteins.
The glycolytic enzyme enolase (EC 4.2.1.11) is active as dimers formed from three subunits encoded by different genes. The embryonic alphaalpha isoform remains distributed in many adult cell types, whereas a transition towards betabeta and gammagamma isoforms occurs in striated muscle cells and neurons respectively. It is not understood why enolase exhibits tissue-specific isoforms with very close functional properties. We approached this problem by the purification of native betabeta-enolase from mouse hindlimb muscles and by raising specific antibodies of high titre against this protein. These reagents have been useful in revealing a heterogeneity of the beta-enolase subunit that changes with in vivo and in vitro maturation. A basic carboxypeptidase appears to be involved in generating an acidic beta-enolase variant, and may regulate plasminogen binding by this subunit. We show for the first time that pure betabeta-enolase binds with high affinity the adjacent enzymes in the glycolytic pathway (pyruvate kinase and phosphoglycerate mutase), favouring the hypothesis that these three enzymes form a functional glycolytic segment. betabeta-Enolase binds with high affinity sarcomeric troponin but not actin and tropomyosin. Some of these binding properties are shared by the alphaalpha-isoenolase, which is also expressed in striated muscle, but not by the neuron-specific gammagamma-enolase. These results support the idea that specific interactions with macromolecules will address muscle enolase isoforms at the subcellular site where ATP, produced through glycolysis, is most needed for contraction. Such a specific targeting could be modulated by post-translational modifications.
Merkulova
T
T
Biochimie Cellulaire, CNRS UPR 9065, Collège de France, 11 place Marcelin Berthelot, 75231 Paris Cedex 05, France.
Lucas
M
M
Jabet
C
C
Lamandé
N
N
Rouzeau
J D
JD
Gros
F
F
Lazar
M
M
Keller
A
A
D000818:Animals; D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006019:Glycolysis; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D009124:Muscle Proteins / Q000302:isolation & purification* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000196:embryology / Q000201:enzymology*; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D011817:Rabbits
D007527:Isoenzymes; D009124:Muscle Proteins; D010958:Plasminogen; D010751:Phosphopyruvate Hydratase