Production of alpha-terpineol from Escherichia coli cells expressing thermostable limonene hydratase.
The genes encoding a thermostable limonene hydratase have been located on a cloned fragment in Escherichia coli conferring growth on limonene and production of the monoterpenes perillyl alcohol and alpha-terpineol. Whole cell bioconversion studies at elevated temperature employing both an aqueous phase and neat limonene phase demonstrated significant production of alpha-terpineol with additional production of carvone.
Savithiry
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Department of Microbiology, Michigan State University, East Lansing 48824, USA.
Cheong
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TK
Oriel
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D019149:Bioreactors; D003001:Cloning, Molecular; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D005798:Genes, Bacterial; D001411:Geobacillus stearothermophilus / Q000235:genetics / Q000378:metabolism; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism*; D000077222:Limonene; D039821:Monoterpenes; D013696:Temperature; D013729:Terpenes / Q000378:metabolism*
D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D013729:Terpenes; C016775:alpha-terpineol; C006923:carvone; D000077222:Limonene; D006836:Hydro-Lyases; C106636:limonene hydratase