title: Age-dependent lens changes in galactose-fed dogs.
abstract: Aldose reductase initiated sugar cataract formation in 9-month old galactose-fed dogs has been documented to progress from an accentuation of lens sutures (1 month after initial feeding) to the appearance of cortical vacuoles (3 months), cortical opacities (4-6 months) and eventually the progressive formation of a clear zone at the cortical equatorial regions of the cataractous lenses (> 12 months). Here, the effect of age on the onset and degree of sugar cataract formation has been investigated in beagles fed a 30% galactose diet starting at 2, 6, and 24 months of age. Cataract formation was monitored by slit lamp and retroillumination microscopy. Compared to 9-month old dogs, cataract formation in the younger dogs was more rapid and the lens changes were more severe. In the 2-month old group of dogs, galactose-feeding resulted in a rapid formation of dense cataracts which began to resorb after 106 days of galactose feeding with only opaque nuclear remnants remaining after eight months. These changes were mirrored by age-dependent reductions of lenticular NADPH-dependent reductase activity.
authors.affiliation: Laboratory of Ocular Therapeutics, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Lackner
authors.forename: L
authors.initials: L
authors.lastname: Rodriguez
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: M J
authors.initials: MJ
authors.lastname: Lizak
authors.forename: M
authors.initials: M
authors.lastname: Wyman
authors.forename: P F
authors.initials: PF
authors.lastname: Kador
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D002386:Cataract / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D004285:Dogs; D005690:Galactose / Q000009:adverse effects*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism
chemical_list: D009247:NADH, NADPH Oxidoreductases; D005690:Galactose