title: Hydrophobic properties of NAD glycohydrolase from neurospora crassa conidia and interaction with dioxane.
abstract: NAD glycohydrolase (NADase, EC 3.2.2.5) from Neurospora crassa conidia shows marked hydrophobic properties which are related to the self inhibition of the enzyme. Both aliphatic amines and carboxylic acids are able to inhibit noncompetitively the catalytic activity of the enzyme and the inhibition depends on the non-polar moiety of the substances. Also dioxane is an inhibitor of NAD glycohydrolase even though it apparently increases the specific activity of the enzyme. This effect can be explained by the fact that NADase is present as a dimer when the enzyme is concentrated or at high temperature, and dioxane binds the enzyme breaking the hydrophobic bonds in the dimeric enzyme and yielding the most active monomeric form which is only slightly inhibited by the organic solvent.
authors.affiliation: Institute of Veterinary Physiology and Biochemistry, University of Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Pace
authors.forename: D
authors.initials: D
authors.lastname: Agnellini
authors.forename: G
authors.initials: G
authors.lastname: Lippoli
authors.forename: R L
authors.initials: RL
authors.lastname: Berger
mesh_terms: D000588:Amines / Q000494:pharmacology; D002151:Calorimetry; D002264:Carboxylic Acids / Q000494:pharmacology; D004146:Dioxanes / Q000378:metabolism* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology*
chemical_list: D000588:Amines; D002264:Carboxylic Acids; D004146:Dioxanes; D004791:Enzyme Inhibitors; D009244:NAD+ Nucleosidase; C025223:1,4-dioxane