title: Elderberry (Sambucus nigra) bark contains two structurally different Neu5Ac(alpha2,6)Gal/GalNAc-binding type 2 ribosome-inactivating proteins.
abstract: A second NeuAc(alpha2,6)Gal/GalNAc binding type 2 ribosome-inactivating protein (RIP), called SNAI' has been isolated from elderberry (Sambucus nigra) bark. SNAI' is a minor bark protein which closely resembles the previously described major Neu5Ac(alpha2,6)Gal/GalNAc binding type 2 RIP called SNAI with respect to its carbohydrate-binding specificity and ribosome-inactivating activity but has a different molecular structure. Molecular cloning revealed that the deduced amino acid sequence of SNAI' is highly similar to that of SNAI and that the difference in molecular structure between both proteins relies on a single cysteine residue present in the B chain of SNAI but absent from SNAI'. The isolation of SNAI' not only identifies a minor bark protein as a type 2 RIP but also further emphasizes the complexity of the type 2 RIP/lectin mixture present in the bark of elderberry.
authors.affiliation: Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Belgium. Els.VanDamme@agr.kuleuven.ac.be
authors.forename: E J
authors.initials: EJ
authors.lastname: Van Damme
authors.forename: S
authors.initials: S
authors.lastname: Roy
authors.forename: A
authors.initials: A
authors.lastname: Barre
authors.forename: L
authors.initials: L
authors.lastname: Citores
authors.forename: K
authors.initials: K
authors.lastname: Mostafapous
authors.forename: P
authors.initials: P
authors.lastname: Rougé
authors.forename: F
authors.initials: F
authors.lastname: Van Leuven
authors.forename: T
authors.initials: T
authors.lastname: Girbés
authors.forename: I J
authors.initials: IJ
authors.lastname: Goldstein
authors.forename: W J
authors.initials: WJ
authors.lastname: Peumans
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D037102:Lectins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009699:N-Glycosyl Hydrolases; D037121:Plant Lectins; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010944:Plants / Q000737:chemistry*; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D016415:Sequence Alignment
chemical_list: D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C050600:Sambucus nigra lectins; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins