Analysis of dissociated single neurons by simple and semi-quantitative RT-PCR (reverse transcription and polymerase chain reaction).
We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H1-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.
Andoh
T
T
Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Tohda
C
C
Kuraishi
Y
Y
D000818:Animals; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011969:Receptors, Histamine H1 / Q000096:biosynthesis
D012333:RNA, Messenger; D011969:Receptors, Histamine H1; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D012194:RNA-Directed DNA Polymerase