title: Determination of U-89968E, a 5HT1a agonist in rat plasma using solid-phase extraction, precolumn derivatization and reversed-phase high-performance liquid chromatography.
abstract: A selective and sensitive HPLC method was developed for the determination of U-39968E in rat plasma. The assay involved solid-phase extraction of the analyte and the internal standard and precolumn derivatization with cyclohexane-1,3-dione reagent before injection on to the HPLC column. The samples were chromatographed on a Spherisorb S5 CN column (25 cm x 4.6 mm i.d.) with a mobile phase containing acetonitrile-trifluoroacetic acid-water (17:0.2:83, v/v/v) at a flow rate of 1.5 ml min-1. The column eluent was monitored by flourescence detection with excitation at 272 nm and emission at 320 nm. The assay is linear over the range 4-759 ng ml-1. The relative standard deviation at the limit of quantification, 4 ng ml-1, was 7.1%. This method was successfully applied to the determination of U-89968E in rat plasma during pharmacokinetic studies.
authors.affiliation: Pharmacia and Upjohn, West Sussex, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Rees
authors.forename: P M
authors.initials: PM
authors.lastname: Austin
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000031:analogs & derivatives* / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017366:Serotonin Receptor Agonists / Q000097:blood*; D013050:Spectrometry, Fluorescence
chemical_list: D017366:Serotonin Receptor Agonists; C106939:U 89968E; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin