The alanine racemase gene is essential for growth of Lactobacillus plantarum.
The Lactobacillus plantarum alr gene encoding alanine racemase was cloned by complementation of an Escherichia coli Alr- DadX- double mutant strain. Knockout of the alr gene abolished all measurable alanine racemase activity, and the mutant was shown to be strictly dependent on D-alanine for growth.
Hols
P
P
Unité de Génétique, Université catholique de Louvain, Belgium.
Defrenne
C
C
Ferain
T
T
Derzelle
S
S
Delplace
B
B
Delcour
J
J
D000411:Alanine Racemase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017353:Gene Deletion; D007778:Lactobacillus / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data
D000411:Alanine Racemase