title: Expression and self-assembly of recombinant capsid protein from the antigenically distinct Hawaii human calicivirus.
abstract: The Norwalk and Hawaii viruses are antigenically distinct members of the family Caliciviridae and are considered to be important etiologic agents of epidemic gastroenteritis, with most studies focusing on the role of Norwalk virus. To further investigate the importance of Hawaii virus, Hawaii virus-like particles (VLPs) were produced by expression of its capsid protein in the baculovirus system and these VLPs were used as the antigen in an enzyme-linked immunosorbent assay that was efficient in the detection of a serologic response to Hawaii virus. The ready availability of Hawaii VLPs should enable larger-scale epidemiological studies to further elucidate the importance of this agent.
authors.affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. kgreen@atlas.niaid.nih.gov
authors.forename: K Y
authors.initials: KY
authors.lastname: Green
authors.forename: A Z
authors.initials: AZ
authors.lastname: Kapikian
authors.forename: J
authors.initials: J
authors.lastname: Valdesuso
authors.forename: S
authors.initials: S
authors.lastname: Sosnovtsev
authors.forename: J J
authors.initials: JJ
authors.lastname: Treanor
authors.forename: J F
authors.initials: JF
authors.lastname: Lew
mesh_terms: D000956:Antigens, Viral / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002139:Caliciviridae / Q000235:genetics*; D002213:Capsid / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D011994:Recombinant Proteins