Low molecular weight Cooperia oncophora antigens. Potential to discriminate between susceptible and resistant calves after infection.
The recognition of low molecular weight proteins by sera obtained during a single oral (primary) infection with 100,000 3rd-stage Cooperia oncophora larvae was studied in calves. Three groups of 6 or 7 calves were selected based on different egg excretion patterns. SDS-gel electrophoresis of adult Cooperia antigen under reducing conditions, followed by Western blotting, revealed that resistance of individual calves to C. oncophora might be related with antibody responses (42 days post infection) against at least 2 protein fragments (14-16 kDa and 27 kDa). The 14-16-kDa protein complex was bound, to some extent, by individual sera from all calves. The intensity of staining was negatively correlated with egg excretion on Day 42 p.i. Calves with high egg counts on Day 21 p.i. either did not or only weakly recognized the 27-kDa band. It has to be established whether the 14-16 kDa (or recombinant 14.2 kDa) provides a tool for immunodiagnostics and whether the 27-kDa fragment can help further unravel immune-mediated resistance to Cooperia.
van Diemen
P M
PM
Department of Animal Husbandry, Animal Health and Reproduction, Agricultural University, Wageningen, The Netherlands.
Ploeger
H W
HW
Nieuwland
M G
MG
Rietveld
F W
FW
Eysker
M
M
Kooyman
F N
FN
Kloosterman
A
A
Parmentier
H K
HK
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000469:parasitology; D016000:Cluster Analysis; D004198:Disease Susceptibility / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015801:Helminth Proteins / Q000276:immunology; D007113:Immunity, Innate; D008970:Molecular Weight; D010270:Parasite Egg Count; D011994:Recombinant Proteins / Q000276:immunology; D014251:Trichostrongyloidea / Q000276:immunology*; D014252:Trichostrongyloidiasis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins