Modulation of neutrophil superoxide response and intracellular diacylglyceride levels by the bacterial pigment pyocyanin.
Low concentrations of pyocyanin are reported to enhance superoxide production by human neutrophils exposed to various stimuli, yet the mechanism remains unknown. Using lucigenin-enhanced chemiluminescence, we examined the kinetics of the neutrophil superoxide response in the presence of pyocyanin. At all concentrations (12.5 to 200 microM), pyocyanin decreased the peak superoxide response while prolonging the duration of the response. The prolonged response may be associated with an observed increase in intracellular diacylglyceride levels due to pyocyanin exposure.
Muller
M
M
Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, New South Wales, Australia.
Sorrell
T C
TC
D004075:Diglycerides / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009249:NADP / Q000378:metabolism; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D011710:Pyocyanine / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*
D004075:Diglycerides; D013481:Superoxides; D009249:NADP; D011710:Pyocyanine