title: Involvement of dsRNA virus in the protein composition and growth kinetics of host Trichomonas vaginalis.
abstract: Trichomonas vaginalis harbors a double-stranded (ds)-RNA virus, and the presence of virus is related to upregulated expression and phenotypic variation of a prominent immunogen (Khoshnan A, Alderete JF (1994) J Virol 68: 4035-4038). To further test the influence of virus on T. vaginalis, virus-infected (V+) isolates were compared to virus-free (V-), agar-cloned progeny trichomonads derived from the parental isolates for accumulation of total proteins and cysteine proteinases. Comparative high resolution two dimensional (2D)-SDS-PAGE was performed of trichomonads grown in a chemostat under identical conditions. At least 47 proteins were identified as specifically expressed by representative V+ isolate 347, and approximately 41 spots were specific to the corresponding V- progeny, showing an association between virus and the presence and absence of parasite proteins. Qualitatively and quantitatively dissimilar cysteine proteinase patterns were detected from numerous V+ isolates and the V- progeny. A 2D analysis for isolate 347 showed the appearance of unique proteinase activities for parental parasites and presence of at least one proteinase in the V- progeny. Finally, the V+ T. vaginalis isolate 347, but not the V- isolate 347 progeny nor other V+ isolates, underwent fluctuations in density during chemostat growth allowing for purification of virus particles from the V+ isolate 347 supernatants during decreased parasite density.
authors.affiliation: Department of Microbiology, University of Texas Health Science Center, San Antonio, USA.
authors.forename: D
authors.initials: D
authors.lastname: Provenzano
authors.forename: A
authors.initials: A
authors.lastname: Khoshnan
authors.forename: J F
authors.initials: JF
authors.lastname: Alderete
mesh_terms: D000818:Animals; D003546:Cysteine Endopeptidases / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D015800:Protozoan Proteins / Q000032:analysis*; D012328:RNA Viruses; D012330:RNA, Double-Stranded; D014246:Trichomonas vaginalis / Q000254:growth & development* / Q000821:virology*
chemical_list: D015800:Protozoan Proteins; D012330:RNA, Double-Stranded; D003546:Cysteine Endopeptidases