Cellular localization and chromosome mapping of a novel candidate tumor suppressor gene (ING1).
A novel gene called ING1 encoding a 33-kDa protein that is an inhibitor of cell growth and a candidate tumor suppressor has been recently isolated (Garkavtsev et al., 1996). Here we show, using indirect immunofluorescence, that the protein (p33ING1) is located in the nucleus, which is consistent with its proposed role as a growth regulator. In addition, we show that a genomic probe to human ING1 localizes to chromosome 13 at q33-->q34 by fluorescence in situ hybridization. This candidate tumor suppressor gene is located near a chromosome region which has been reported to be a site for translocation and deletion in gastric cancers and head and neck squamous carcinomas.
Garkavtsev
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I
Department of Medical Biochemistry, Southern Alberta Cancer Research Centre, University of Calgary, Canada. igarkavt@cs.ucalgary.ca
Demetrick
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Riabowol
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D000818:Animals; D018797:Cell Cycle Proteins; D002460:Cell Line; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D004268:DNA-Binding Proteins; D016147:Genes, Tumor Suppressor; D006131:Growth Inhibitors / Q000235:genetics*; D006801:Humans; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011817:Rabbits; D025521:Tumor Suppressor Proteins
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D006131:Growth Inhibitors; C501503:ING1 protein, human; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D011506:Proteins; D025521:Tumor Suppressor Proteins