Blockade of pilocarpine- or kainate-induced mossy fiber sprouting by cycloheximide does not prevent subsequent epileptogenesis in rats.
Post-injury sprouting of hippocampal mossy fibers has been suggested to be a causal mechanism underlying the development of temporal lobe epilepsy. However, this hypothesis rests entirely on indirect correlational evidence. Here we demonstrate that cycloheximide, a protein synthesis inhibitor, blocked pilocarpine- and kainate-induced mossy fiber sprouting in rats, but did not prevent the subsequent development of spontaneous seizures or affect their frequency. These results provide direct evidence against a causal role for mossy fiber sprouting in temporal lobe epileptogenesis.
Longo
B M
BM
Department of Physiology, Universidade Federal de São Paulo-EPM, Brazil.
Mello
L E
LE
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004569:Electroencephalography / Q000187:drug effects; D004827:Epilepsy / Q000517:prevention & control*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D007608:Kainic Acid / Q000037:antagonists & inhibitors*; D008297:Male; D018721:Muscarinic Agonists / Q000494:pharmacology*; D009412:Nerve Fibers / Q000187:drug effects; D010862:Pilocarpine / Q000037:antagonists & inhibitors*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D018690:Excitatory Amino Acid Agonists; D018721:Muscarinic Agonists; D011500:Protein Synthesis Inhibitors; D010862:Pilocarpine; D003513:Cycloheximide; D007608:Kainic Acid