title: The acid lipase gene family: three enzymes, one highly conserved gene structure.
abstract: Human gastric lipase (HGL; triacylglycerol lipase; EC 3.1.1.3) plays an important role in the digestion of dietary triglycerides in the gastrointestinal tract, especially in patients suffering from pancreatic lipase deficiencies. The enzyme is secreted by the fundic mucosa of the stomach and hydrolyzes the ester bonds of triglycerides under acidic pH conditions, while cholesteryl esters are not attacked. The 379-amino acid protein is highly homologous to two other acidic lipases, rat lingual lipase (RLL; triacylglycerol lipase; EC 3.1.1.3) and human lysosomal acid lipase (HLAL; cholesteryl esterase; EC 3.1.1.13). To determine whether this remarkable similarity is also present at the genomic level, we have elucidated the respective gene structures by screening three bacteriophage lambda libraries and by polymerase chain reaction-based intron amplification. The genes encoding HGL, RLL, and HLAL are composed of 10 exons interrupted by nine introns and span about 14 kb, 18.7 kb, and 38.8 kb of genomic DNA, respectively. The HGL and RLL gene organizations are identical, suggesting that RLL is the rat gastric lipase expressed in the serous von Ebner glands of the tongue. The positions of the HLAL intervening sequences are also absolutely conserved, except for the location of intron 1. Our results support the concept that HLAL and HGL/RLL are members of a gene family of lipases that most likely have evolved by duplication of an ancestral gene and subsequently assumed distinct roles in neutral lipid metabolism due to sequence divergence and different expression patterns.
authors.affiliation: Department of Clinical Chemistry, Grosshadern Clinic, University of Munich, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Lohse
authors.forename: P
authors.initials: P
authors.lastname: Lohse
authors.forename: S
authors.initials: S
authors.lastname: Chahrokh-Zadeh
authors.forename: D
authors.initials: D
authors.lastname: Seidel
mesh_terms: D000818:Animals; D001483:Base Sequence; D006801:Humans; D008049:Lipase / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D002787:Sterol Esterase / Q000235:genetics*
chemical_list: D002787:Sterol Esterase; D008049:Lipase