title: Expression of interstitial collagenase (matrix metalloproteinase-1) in gastric cancers.
abstract: The expression of matrix metalloproteinase-1 (MMP-1) gene and the presence of MMP-1 protein in gastric cancer were examined by in situ hybridization and immunohistochemistry. Expression of the interstitial collagenase (MMP-1) gene was detected within the stroma of the neoplastic glands, and infiltration of eosinophils was observed to be associated with regions of MMP-1 gene expression. The degree of eosinophilic infiltration correlated with the level of MMP-1 mRNA expression. Immunostaining showed localization of MMP-1 protein in the stromal cells, and additionally in the neoplastic glands. These findings indicate that the stromal cells may play an important role in the expression of MMP-1, and suggest a pathophysiological role for MMP-1 in the invasion and metastasis of gastric cancer.
authors.affiliation: Department of Surgery, School of Medicine, Keio University, Shinjuku-ku, Tokyo.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakurai
authors.forename: Y
authors.initials: Y
authors.lastname: Otani
authors.forename: K
authors.initials: K
authors.lastname: Kameyama
authors.forename: Y
authors.initials: Y
authors.lastname: Hosoda
authors.forename: I
authors.initials: I
authors.lastname: Okazaki
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: K
authors.initials: K
authors.lastname: Kumai
authors.forename: M
authors.initials: M
authors.lastname: Kitajima
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D017364:Collagenases / Q000378:metabolism*; D004804:Eosinophils / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D017403:In Situ Hybridization; D020781:Matrix Metalloproteinase 1; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D017364:Collagenases; D020781:Matrix Metalloproteinase 1