title: Lymphocyte proliferative responses of goats vaccinated with Brucella melitensis 16M or a delta purE201 strain.
abstract: The response to a Brucella melitensis purEK deletion mutant, delta purE201 (referred to as strain 201), was compared with the response to its parental strain, 16M, in juvenile goats. Proliferative responses to gamma-irradiated bacteria were detected earlier in strain 201-infected goats. Lymphocytes from strain 16M- or 201-infected goats proliferated in response to one-dimensional polyacrylamide gel electrophoresis-separated proteins of similar mass isolated from strain 16M or Brucella abortus RB51. Data from this study suggest that some antigens stimulating cell-mediated responses are conserved among Brucella species, as 201- and 16M-infected goats recognized similar proteins expressed by RB51 and 16M.
authors.affiliation: National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010, USA. SOlsen@nadc.ars.usda.com
authors.forename: S C
authors.initials: SC
authors.lastname: Olsen
authors.forename: N F
authors.initials: NF
authors.lastname: Cheville
authors.forename: M G
authors.initials: MG
authors.lastname: Stevens
authors.forename: H H
authors.initials: HH
authors.lastname: Houng
authors.forename: E S
authors.initials: ES
authors.lastname: Drazek
authors.forename: T L
authors.initials: TL
authors.lastname: Hadfield
authors.forename: R L
authors.initials: RL
authors.lastname: Warren
authors.forename: D L
authors.initials: DL
authors.lastname: Hoover
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D017347:Brucella melitensis / Q000276:immunology* / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006041:Goats / Q000276:immunology*; D008213:Lymphocyte Activation; D014611:Vaccination / Q000662:veterinary
chemical_list: D001428:Bacterial Vaccines