title: Molecular detection and identification of Paracoccidioides brasiliensis.
abstract: Nearly 800 nucleotides from the 5' terminus of the 28S ribosomal gene of Paracoccidioides brasiliensis were sequenced, and a 14-base DNA probe specific for this species was identified. Hybridization results showed that the probe identified P. brasiliensis ribosomal DNA in a panel of ribosomal DNAs representing a total of 48 species of fungi.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
authors.forename: G S
authors.initials: GS
authors.lastname: Sandhu
authors.forename: R A
authors.initials: RA
authors.lastname: Aleff
authors.forename: B C
authors.initials: BC
authors.lastname: Kline
authors.forename: C
authors.initials: C
authors.lastname: da Silva Lacaz
mesh_terms: D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010228:Paracoccidioides / Q000145:classification* / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal