title: Regulation of fibrillar collagen gene expression and protein accumulation in volume-overloaded cardiac hypertrophy.
abstract: BACKGROUND
  Interstitial collagen accumulation has been extensively demonstrated to be increased at both mRNA and protein levels in pressure-overloaded cardiac hypertrophy. However, few data are available regarding the effects of volume overload on myocardial collagens.
  
  
  RESULTS
  To determine whether the alterations of collagens may occur in volume-overloaded cardiac hypertrophy, we measured collagen types I and III mRNA levels and protein accumulation in left ventricular (LV) myocardium of rats at 3, 7, and 28 days after the creation of an aortocaval (AC) shunt. Eccentric LV hypertrophy was produced in rats with AC shunting. Northern blot analysis on RNA extracted from LV tissue indicated that the steady state mRNA levels for both type I and III collagen were persistently upregulated in AC shunt rats compared with sham-operated operated control rats. In contrast, the biochemical collagen protein concentration and morphometric collagen volume fraction were comparable between sham-operated control and AC shunt rats at any study time point. Furthermore, the immunohistochemical staining of types I and III collagen and Sirius red staining on myocardial tissue sections revealed no significant alterations in the distribution or density of fibrillar collagens in AC shunt rats. Tissue collagenase activity was not different between control and AC shunt rats after 28 days.
  
  
  CONCLUSIONS
  Cardiac volume overload increases LV collagen mRNA as does pressure overload. However, in contrast to pressure-overloaded hypertrophy, the upregulation of collagen transcriptional activity does not result in subsequent myocardial fibrosis in volume-overloaded hypertrophy due to AC shunting. Therefore, the upregulation of collagen gene expression and protein accumulation might be different in pressure-overloaded and volume overloaded hypertrophy.
authors.affiliation: Research Institute of Angiocardiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Namba
authors.forename: H
authors.initials: H
authors.lastname: Tsutsui
authors.forename: H
authors.initials: H
authors.lastname: Tagawa
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Kozai
authors.forename: M
authors.initials: M
authors.lastname: Usui
authors.forename: K
authors.initials: K
authors.lastname: Imanaka-Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Imaizumi
authors.forename: A
authors.initials: A
authors.lastname: Takeshita
mesh_terms: D000818:Animals; D006332:Cardiomegaly / Q000235:genetics* / Q000378:metabolism*; D003094:Collagen / Q000235:genetics* / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism; D015870:Gene Expression; D006940:Hyperemia / Q000150:complications*; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009210:Myofibrils / Q000502:physiology*; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003094:Collagen; D017364:Collagenases