Serum-free medium and pyruvate improve survival and glucose responsiveness of islet beta cells in culture.
Bottino
R
R
Diabetes Research Institute, University of Miami, School of Medicine, Florida, USA.
Inverardi
L
L
Valente
U
U
Ricordi
C
C
D000293:Adolescent; D000328:Adult; D000818:Animals; D001769:Blood; D002102:Cadaver; D002417:Cattle; D002470:Cell Survival; D003470:Culture Media; D016895:Culture Media, Serum-Free; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008875:Middle Aged; D019289:Pyruvic Acid; D014019:Tissue Donors; D014021:Tissue Preservation / Q000379:methods*
D003470:Culture Media; D016895:Culture Media, Serum-Free; D007328:Insulin; D019289:Pyruvic Acid; D005947:Glucose