Parasite antigen-induced IFN-gamma and IL-4 production by cells from pathopermissive and pathoresistant strains of mice infected with Trypanosoma cruzi.
The cardiac pathology associated with infection by Trypanosoma cruzi in mice has been suggested to be partially dependent upon cytokine responses. The pathoresistant B10.D2 mice, which display little infection-induced myocarditis, and the pathopermissive DBA/2 mice, which show significant cardiac damage, were compared for their in vitro interferon (IFN)-gamma and interleukin (IL)-4 production. Concanavalin A-stimulated spleen cells from infected B10.D2 mice produce a greater amount of IFN-gamma than DBA/2 mice, whereas the IL-4 production is only slightly greater in the B10.D2 mice than the DBA/2 mice. Parasite antigen stimulation of spleen cells from these mice results in a clearly greater IFN gamma production by the B10.D2 and a higher IL-4 level for the DBA/2 mice. The data presented suggest a relationship between an enhanced TH1-type response and decreased chronic cardiac pathogenesis, whereas a lower level of TH1 activity may play a role in cardiac involvement.
Humphrey
J S
JS
Tropical Disease Institute, College of Osteopathic Medicine, Ohio University, Athens 45701, USA.
McCormick
T S
TS
Rowland
E C
EC
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D002478:Cells, Cultured; D002598:Chagas Cardiomyopathy / Q000276:immunology; D014355:Chagas Disease / Q000276:immunology*; D003208:Concanavalin A; D004198:Disease Susceptibility; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D018512:Parasitemia / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology
D000953:Antigens, Protozoan; D003208:Concanavalin A; D015847:Interleukin-4; D007371:Interferon-gamma