ATP/ADP antiporter- and aspartate/glutamate antiporter-mediated fatty acid-induced uncoupling of liver mitochondria in incubation media differing in ion composition.
Substitution of potassium cloride for sucrose in the incubation medium of liver mitochondria or addition of magnesium cloride to the sucrose medium produces a small (if any) effect on the palmitate-induced uncoupling and its sensitivity to carboxyatractylate and aspartate, but it exerts a pronounced increase in the recoupling effect of glutamate on this uncoupling. 20-40 microM cetyltrimethyl ammonium bromide increases the recoupling effect of glutamate and aspartate but decreases that of carboxyatractylate. The data are in line with suggestion that both ATP/ADP antiporter and aspartate/glutamate antiporters are involved in the fatty acid-induced uncoupling.
Samartsev
V N
VN
Mari State University, Yoshkar-Ola.
Mokhova
E N
EN
D026922:Amino Acid Transport Systems, Acidic; D000818:Animals; D017920:Antiporters; D001224:Aspartic Acid / Q000494:pharmacology; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D015636:Magnesium Chloride / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D019308:Palmitic Acid / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology*
D026922:Amino Acid Transport Systems, Acidic; D017920:Antiporters; D002352:Carrier Proteins; D002593:Cetrimonium Compounds; D014475:Uncoupling Agents; C051046:aspartate-glutamate carrier; D015636:Magnesium Chloride; D001278:Atractyloside; D019308:Palmitic Acid; D001224:Aspartic Acid; D018698:Glutamic Acid; D011189:Potassium Chloride; D000226:Mitochondrial ADP, ATP Translocases; C003853:carboxyatractyloside; D000077286:Cetrimonium