Molecular genetic analysis of HLA class II alleles in Japanese patients with melanoma.
Distribution of HLA-DQA, -DQB and -DPB alleles in ninety-six Japanese patients with melanoma was analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and the association between clinical parameters and the presence of certain HLA class II alleles investigated. The frequency of HLA-DQB1*0302 was increased, while those of DQA1*0101(04), -DQA1*0401 and DRB1*0802 were decreased in melanoma patients compared with controls. Moreover, the frequency of HLA-DQA1*0103 in patients with acral lentiginous melanoma was increased compared with controls. However, none of these HLA class II alleles showed significant positive or negative associations after correction of the P value. In addition, there was no correlation between these antigens and clinical parameters. These results indicate that HLA class II alleles may not contribute to a strong susceptibility to melanoma in the Japanese.
Kageshita
T
T
Department of Dermatology, Kumamoto University School of Medicine, Japan. toshiro@kaiju.medic.kumamoto-u.ac.jp
Naruse
T
T
Hirai
S
S
Ono
T
T
Horikoshi
T
T
Nakagawa
H
H
Tamaki
K
K
Hayashibe
K
K
Ichihashi
M
M
Nakayama
J
J
Hori
Y
Y
Ozawa
A
A
Miyahara
M
M
Ohkido
M
M
Inoko
H
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D007564:Japan; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged
D000949:Histocompatibility Antigens Class II