[Use of catalytic properties of iron-containing salts in immunoanalysis].
The possibility of using inorganic catalysts as markers for solid-phase immunoassay on the plates is shown with iron-containing hydrosols: ferrum hydroxide, ammonium ferriglycerate, and ferrum hydroxide modified with potassium ferrocyanide. Diagnostic agents based on iron-containing sols with dispersion of 0.5 to 0.8 mm bound to immunoglobulins or staphylococcal protein A are no less sensitive than the immunoperoxidase agents, provided the same developing system is used (o-phenylenediamine + hydrogen peroxide). The sensitivity of solid-phase immunocatalytical assay of purified vaccinia and Venezuelan equine encephalomyelitis virus antigens was about 2 and 10 ng/ml, respectively. Hence, inorganic catalysts are prospective markers for immunoassay.
Poltavchenko
A G
AG
Sen'kin
Iu N
IuN
Karavaeva
V S
VS
D000956:Antigens, Viral / Q000032:analysis; D015415:Biomarkers; D002384:Catalysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000737:chemistry; D007202:Indicators and Reagents; D058085:Iron Compounds / Q000737:chemistry; D012492:Salts; D012680:Sensitivity and Specificity; D013205:Staphylococcal Protein A / Q000737:chemistry; D014777:Virus Diseases / Q000175:diagnosis
D000956:Antigens, Viral; D015415:Biomarkers; D007136:Immunoglobulins; D007202:Indicators and Reagents; D058085:Iron Compounds; D012492:Salts; D013205:Staphylococcal Protein A