title: A functional neo-centromere formed through activation of a latent human centromere and consisting of non-alpha-satellite DNA.
abstract: We recently described a human marker chromosome containing a functional neo-centromere that binds anti-centromere antibodies, but is devoid of centromeric alpha-satellite repeats and derived from a hitherto non-centromeric region of chromosome 10q25. Chromosome walking using cloned single-copy DNA from this region enabled us to identify the antibody-binding domain of this centromere. Extensive restriction mapping indicates that this domain has an identical genomic organization to the corresponding normal chromosomal region, suggesting a mechanism for the origin of this centromere through the activation of a latent centromere that exists within 10q25.
authors.affiliation: Murdoch Institute for Research into Birth Defects, Royal Children's Hospital, Parkville, Australia.
authors.forename: D
authors.initials: D
authors.lastname: du Sart
authors.forename: M R
authors.initials: MR
authors.lastname: Cancilla
authors.forename: E
authors.initials: E
authors.lastname: Earle
authors.forename: J I
authors.initials: JI
authors.lastname: Mao
authors.forename: R
authors.initials: R
authors.lastname: Saffery
authors.forename: K M
authors.initials: KM
authors.lastname: Tainton
authors.forename: P
authors.initials: P
authors.lastname: Kalitsis
authors.forename: J
authors.initials: J
authors.lastname: Martyn
authors.forename: A E
authors.initials: AE
authors.lastname: Barry
authors.forename: K H
authors.initials: KH
authors.lastname: Choo
mesh_terms: D001666:Binding Sites, Antibody; D002503:Centromere; D018244:Chromosomes, Artificial, Yeast; D002879:Chromosomes, Human, Pair 10; D004276:DNA, Satellite / Q000235:genetics* / Q000378:metabolism; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence
chemical_list: D004276:DNA, Satellite; D005819:Genetic Markers