Novel green fluorescent protein (GFP) baculovirus expression vectors.
Baculovirus expression vectors were constructed, which contained gfp as a reporter gene. Substitutions in the amino acid sequences were carried out to produce two mutant forms of GFP. One of these mutants produces blue color when excited by ultraviolet (UV) light. The other mutant produces a yellow color that can be visualized in regular daylight. The gene of interest cloned in-frame with the gfp open reading frame allows visualization of the produced GFP-fusion protein using UV light. The presence of an in-frame 6 x His tag between the gfp cDNA and the multiple cloning site allows purification of the fusion protein on a Ni-NTA (nickel-nitrilo-triacetic acid) agarose matrix.
Wu
C
C
Department of Molecular and Cellular Biology, PharMingen, San Diego, CA 92121, USA.
Liu
H
H
Crossen
R
R
Gruenwald
S
S
Singh
S
S
D000595:Amino Acid Sequence; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase; D003001:Cloning, Molecular; D018076:DNA, Complementary; D049308:Dioxygenases; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010105:Oxygenases / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics
D018076:DNA, Complementary; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase