Parasite genome analysis. A global map of the Leishmania major genome: prelude to genomic sequencing.
In 1994, the World Health Organization (TDR) launched a new strategic initiative in parasite genome analysis, establishing international genome networks for filariae, Schistosoma, Leishmania, Trypanosoma brucei and T. cruzi. For Leishmania, a number of different but complementary approaches have been adopted by members of the Leishmania Genome Network. Our laboratory has been using cosmid clone fingerprinting to produce a physical map of the genome. Progress towards the completion of an integrated physical and biological map of L. major, and the preparations for genomic sequencing, are described.
Ivens
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Department of Biochemistry, Imperial College, London, UK.
Smith
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D000818:Animals; D003195:Computer Communication Networks; D003360:Cosmids; D016172:DNA Fingerprinting; D015342:DNA Probes; D016208:Databases, Factual; D001330:Electronic Data Processing; D016521:Electrophoresis, Gel, Pulsed-Field; D018503:Genome, Protozoan; D015698:Genomic Library; D018320:Leishmania major / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D016324:Sequence Tagged Sites
D015342:DNA Probes