The mechanism of anticoagulant activity of a novel heparinoid sulfated glucoside-bearing polymer.
In our previous study on glycoside polymer, it was discovered that sulfated poly(glucosyloxyethyl methacrylate) [poly(GEMA)-sulfate], which bears sulfated D-(+)-glucose, exhibited anticoagulant activity. The anticoagulant activity of poly(GEMA)-sulfate in solution was prolonged by increasing the dose or degree of sulfation of the polymer. In this study, to recognize the mechanism of anticoagulant activity of poly(GEMA)-sulfate, we estimated the binding capacity of antithrombin III to thrombin and some in vitro clotting tests in the presence of poly(GEMA)-sulfate. These results revealed that poly(GEMA)-sulfate had an anticoagulant mechanism which differed to that of heparin. We concluded that the anticoagulant activity of poly(GEMA)-sulfate is responsible for inhibiting fibrin network formation by the insoluble ion complex between fibrinogen and poly(GEMA)-sulfate.
Sakamoto
N
N
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
Kishida
A
A
Maruyama
I
I
Akashi
M
M
D000925:Anticoagulants / Q000494:pharmacology*; D000990:Antithrombin III / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D010314:Partial Thromboplastin Time; D011108:Polymers / Q000494:pharmacology*; D011485:Protein Binding; D011517:Prothrombin Time; D013053:Spectrophotometry / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013463:Sulfuric Acid Esters / Q000494:pharmacology*; D013917:Thrombin / Q000378:metabolism*; D013918:Thrombin Time
D000925:Anticoagulants; D005960:Glucosides; D011108:Polymers; D013463:Sulfuric Acid Esters; C090633:poly(glucosylethyl methacrylate); D000990:Antithrombin III; D005340:Fibrinogen; D006493:Heparin; D013917:Thrombin