title: Effects of intestinal stasis on intercellular adhesion molecule 1 expression in the rat: role of enteric bacteria.
abstract: OBJECTIVE
  The mechanisms underlying the inflammatory changes associated with intestinal stasis are poorly understood. The objective of this study was to assess whether endothelial expression of intercellular adhesion molecule 1 (ICAM-1) and leukocyte recruitment are altered after intestinal stasis.
  
  
  METHODS
  ICAM-1 expression and granulocyte recruitment were quantified in different tissues of Sprague-Dawley rats using the double-radiolabeled monoclonal antibody technique and peroxidase activity, respectively.
  
  
  RESULTS
  Both constitutive and endotoxin-induced ICAM-1 expression were significantly higher in the cecum than in distal colon, a finding that cannot be explained by a difference in endothelial surface area between the two organs. Surgical procedures to improve cecal stool flow (cecostomy, ileocecostomy) elicited a significant decrease in constitutive ICAM-1 expression in both cecum and distal colon. Tissue peroxidase activity was normally higher in cecum than in distal colon, and this difference was significantly reduced by ileocecostomy. Oral administration of antibiotics (kanamycin and/or metronidazole for 2 days) significantly reduced constitutive ICAM-1 expression in the cecum, but not in the distal colon.
  
  
  CONCLUSIONS
  This study indicates that intestinal stasis is associated with an increased expression of ICAM-1 and granulocyte infiltration, which may be mediated by enteric bacteria.
authors.affiliation: Department of Physiology, Louisiana State University Medical Center, Shreveport 71130-3932, USA.
authors.forename: S
authors.initials: S
authors.lastname: Komatsu
authors.forename: J
authors.initials: J
authors.lastname: Panés
authors.forename: M B
authors.initials: MB
authors.lastname: Grisham
authors.forename: J M
authors.initials: JM
authors.lastname: Russell
authors.forename: N
authors.initials: N
authors.lastname: Mori
authors.forename: D N
authors.initials: DN
authors.lastname: Granger
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000502:physiology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018799:Intercellular Adhesion Molecule-1