Cloning of human chromosome 17-specific cDNAs using representational difference analysis and human-mouse hybrid cells.
We employed cDNA representational difference analysis (RDA) with human-mouse somatic hybrid cells containing human chromosome 17 and obtained several cDNA clones specific for this chromosome. A cDNA library from PHA-stimulated T cells was screened with unknown cDNA clones obtained by RDA as probes. Subsequently, 1 complete gene and 1 partial cDNA clone were obtained. Our successful result implies that this subtractive amplification technique with hybrid cells will be a useful aid in positional cloning in large-spanning regions.
Tajima
Y
Y
Department of Neurology, Hokkaido University School of Medicine, Sapporo, Japan.
Tashiro
K
K
Camerini
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005784:Gene Amplification; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes
D018076:DNA, Complementary; D012333:RNA, Messenger