Correlation between the adaptive response and individual sensitivity to monoepoxybutene in in vitro experiments on human lymphocytes.
Individual variations in the susceptibility to mutagenic/carcinogenic chemicals depend on the activity of xenobiotic metabolizing enzymes and on DNA- and chromosome-damage repair systems. Monoepoxybutene (MEB) is a genotoxic metabolite of 1,3-butadiene (BD), which has been classified as a probable carcinogen in humans. The purpose of the present study was to investigate by in vitro experiments on human whole blood lymphocytes (WBL), whether an individual sensitivity to MEB correlates with the adaptive response to the tested agent. In the analyzed group, 8.3% of blood donors were relatively sensitive to MEB. The comparison of SCE induction in cultures pretreated and not pretreated with an adaptive dose (AD) of MEB showed, that there was an adaptive response to MEB. The adaptive response in the group of relatively sensitive donors was similar to that of the relatively resistant ones. This result suggests that individual sensitivity to the tested agent and adaptive response depend on different biological mechanisms.
Sasiadek
M
M
Genetic Department Wroclaw University of Medicine, Poland.
Paprocka-Borowicz
M
M
D000222:Adaptation, Physiological; D000328:Adult; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000633:toxicity*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D012680:Sensitivity and Specificity; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D004852:Epoxy Compounds; D009153:Mutagens; C024370:3,4-epoxy-1-butene