Detection of 2-amino-5-chloropyridine in urine as a parameter of zopiclone (Imovane) intake using HPLC with diode array detection.
A qualitative screening technique was developed for the detection of 2-amino-5-chloropyridine, a newly identified decomposition product of zopiclone and metabolites after alkaline hydrolysis of urine samples. The method was elaborated using a standard operation procedure (Merck Tox Screening System), combining a solid-phase extraction with reversed-phase high-performance liquid chromatography and diode array detection. The limit of detection, expressed as zopiclone concentrations in spiked urine, was found to be 0.5 microg/mL. Field urine samples from a volunteer were positive for 2-amino-5-chloropyridine until 16 h after ingestion of one therapeutic dose of Imovane (7.5 mg zopiclone).
Mannaert
E
E
Laboratory of Toxicology, Katholieke Universiteit Leuven, Belgium.
Tytgat
J
J
Daenens
P
P
D000284:Administration, Oral; D053961:Azabicyclo Compounds; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006993:Hypnotics and Sedatives / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine; D010879:Piperazines / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine; D011725:Pyridines / Q000652:urine*; D012015:Reference Standards
D053961:Azabicyclo Compounds; D006993:Hypnotics and Sedatives; D010879:Piperazines; D011725:Pyridines; C515050:zopiclone; C108972:2-amino-5-chloropyridine