Nucleotide sequence analysis of the HLA class I region spanning the 237-kb segment around the HLA-B and -C genes.
To elucidate the detailed gene organization of the human leukocyte antigen (HLA) class I region on chromosome 6, seven contiguous cosmid genomic clones covering the 237-kb segment around the HLA-B and -C loci were subjected to DNA sequencing by the shotgun strategy to give a single contig of 236,822 bp from the MICA gene (58.2 kb centromeric of HLA-B) to 90.8 kb telomeric of HLA-C. This region was confirmed to contain four known genes, MICA, HLA-17, HLA-B, and HLA-C, from centromere to telomere. Further, a new member of the P5 multicopy genes was found to be about 1.3 kb upstream of the HLA-17 gene and designated P5.8. Five novel genes designated NOB1-5 were identified by RT-PCR and Northern blot hybridization. In addition, two pseudogenes, dihydrofolate reductase pseudogene (DHFRP) and ribosomal protein L3 homologous gene (RPL3-Hom), were also found in the vicinity of the HLA-B and -C genes, respectively. The two segments (about 40 kb) downstream of the HLA-B and HLA-C genes showed high sequence homology to each other, suggesting that segmental genome duplication including the major histocompatibility complex (MHC) class I gene must have occurred during the evolution of the MHC.
Mizuki
N
N
Department of Genetic Information, Tokai University School of Medicine, Kanagawa, Japan.
Ando
H
H
Kimura
M
M
Ohno
S
S
Miyata
S
S
Yamazaki
M
M
Tashiro
H
H
Watanabe
K
K
Ono
A
A
Taguchi
S
S
Sugawara
C
C
Fukuzumi
Y
Y
Okumura
K
K
Goto
K
K
Ishihara
M
M
Nakamura
S
S
Yonemoto
J
J
Kikuti
Y Y
YY
Shiina
T
T
Chen
L
L
Ando
A
A
Ikemura
T
T
Inoko
H
H
D015152:Blotting, Northern; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000235:genetics; D019143:Evolution, Molecular; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D000090822:Ribosomal Protein L3; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D015235:HLA-B Antigens; D015236:HLA-C Antigens; D012333:RNA, Messenger; C000716547:RPL3 protein, human; D000090822:Ribosomal Protein L3; D004247:DNA