Production and characterization of monoclonal antibodies against Taylorella equigenitalis.
Monoclonal antibodies were produced against Taylorella equigenitalis using two reference strains. Out of the 79 hybridoma clones shown to express antibodies to T equigenitalis by indirect immunofluorescence assay, 16 were selected for monoclonal antibody production and characterization. These clones recognized different field strains of T equigenitalis isolated in France. They showed no cross-reaction with bacterial strains with previously reported antigenic cross-reactivity, nor did they react with other bacteria commonly found in genital flora. The epitopes recognized by eight of the monoclonal antibodies were situated in proteins of 150, 120, 52.7 and 22 kDa. These epitopes were resistant to the extraction denaturing conditions. These monoclonal antibodies could be used as reagents for specific detection of T equigenitalis.
Gradinaru
D A
DA
Laboratoire vétérinaire départemental de l'Orne, Alençon, France. Congen61@worldnet.fr
Helmer
J M
JM
Klein
F
F
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004716:Endometritis / Q000175:diagnosis / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006190:Haemophilus / Q000276:immunology*; D006192:Haemophilus Infections / Q000175:diagnosis / Q000662:veterinary; D006734:Horse Diseases / Q000175:diagnosis; D006736:Horses; D006825:Hybridomas; D015151:Immunoblotting / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes