Our experience with the enhanced polymer one-step staining in frozen sections.
The enhanced polymer one-step staining (EPOS) method is presented with minor modifications for intraoperative frozen sections. It can be applied in almost every department of pathology where there is a necessity, because no special equipment is required. The method is rapid and immunostaining can be performed in less than 15 min. Antibodies are available for more than 20 important tissue and tumor markers. Out of those, antibodies against human cytokeratin, leukocyte common antigen (LCA) and chromogranin A were introduced in routine work. The first 2 antibodies yield excellent results and are a big advantage in routine diagnostic work: the use of cytokeratin is useful for confirmation or exclusion of suspected tumor invasion, especially in cases of atypical hyperplastic lesions, for detection of spindle cell carcinomas, and the detection of lymph node micrometastases. LCA demonstration is helpful in differentiating various round cell tumors, such as poorly differentiated carcinomas, sarcomas, malignant lymphomas, and evaluation of inflammatory response. Chromogranin A may detect neuroendocrine differentiation in the tumors.
Rott
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T
Institute of Pathology, Medical Faculty, Ljubljana, Slovenia. rott@ibmi.mf.uni-lj.si
Velkavrh
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D004848:Epithelium / Q000473:pathology; D005629:Frozen Sections; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry / Q000379:methods*; D007430:Intraoperative Care; D009369:Neoplasms / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods*