title: Molecular analysis of FMRFamide- and FMRFamide-related peptides (FaRPS) in the cuttlefish Sepia officinalis.
abstract: The display of complex color patterns of the cuttlefish Sepia officinalis is under the regulation of the FMRFamide-related peptide (FaRP) family, but their exact identities are unknown. We report the isolation and characterization of a full-length FaRP cDNA from the brain of S. officinalis. This cDNA is 1850 base pairs long, including an open reading frame of 996 base pairs. The cDNA encodes a precursor protein containing four FaRPs: ALSGDAFLRF, FIRF, FLRF and FMRF. Each propeptide has a C-terminal glycine residue that is presumably converted post-translationally to an amide. Every FaRP propeptide is also flanked by basic amino acid residues at the amino and carboxy termini, indicative of putative cleavage sites during post-translational processing. Each of the four FaRPs encoded by this cDNA causes chromatophore expansion when assayed in an in vitro chromatophore bioassay. Thus, it is likely that one or more of the FaRPs identified in this study are involved in controlling chromatophore activity in cuttlefish.
authors.affiliation: Institute of Neuroscience, University of Oregon, Eugene 97403, USA. Loi@uoneuro.uoregon.edu
authors.forename: P K
authors.initials: PK
authors.lastname: Loi
authors.forename: N
authors.initials: N
authors.lastname: Tublitz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D019835:FMRFamide; D008969:Molecular Sequence Data; D008974:Mollusca / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics*; D018377:Neurotransmitter Agents / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; C103585:FMRFamide precursor; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D011498:Protein Precursors; D019835:FMRFamide