UVB-induced formation of (6-4) photoproducts in the rat corneal epithelium.
The induction of DNA photoproducts in rat corneal epithelium was studied after in vivo exposure to different doses of ultraviolet B light at 297 nm. Affinity-purified antibodies with a major specificity against UV-induced (6-4) photoproducts were used. The results indicate a dose dependent formation of (6-4) photoproducts. Even a minimal erythema dose (25 mJ/cm2) produced (6-4) photoproducts, demonstrating that DNA damage occurs in corneal tissue following exposure to biologically relevant doses of UVB light.
Estil
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Department of Ophthalmology, University of Oslo, Rikshospitalet, Norway.
Olsen
W M
WM
Huitfeldt
H S
HS
Haaskjold
E
E
D000818:Animals; D003315:Cornea / Q000378:metabolism / Q000528:radiation effects*; D004247:DNA / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004848:Epithelium / Q000378:metabolism / Q000528:radiation effects; D019793:Fluorescein; D005452:Fluoresceins; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000528:radiation effects; D011740:Pyrimidine Dimers / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014466:Ultraviolet Rays
D005452:Fluoresceins; D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer; D004247:DNA; D019793:Fluorescein