Cockroach allatostatin-like immunoreactivity in the central nervous system of the freshwater snails Bulinus globosus (Planorbidae) and Stagnicola elodes (Lymnaeidae).
Allatostatin-like immunoreactivity was demonstrated in the central nervous system (CNS) neurons of the freshwater pulmonate snails Bulinus globosus (Planorbidae) and Stagnicola elodes (Lymnaeidae). Immunopositive neurons were localized using a monoclonal antibody highly specific to allatostatin I (APSGAQRLYGFGL-amide) from the cockroach Diploptera punctata. All CNS ganglia in both snail species contained immunopositive neurons. The pedal ganglia showed large numbers (80-100) of neurons in Bulinus and Stagnicola. Large numbers of immunopositive cells were also found in the cerebral (60) and buccal ganglia (20) of Bulinus. Other ganglia contained fewer immunopositive cells, but these cells were most concentrated in the cerebral (Stagnicola) and left parietal (Bulinus) ganglia and the visceral ganglia of both species. The high concentration of immunopositive cells in the pedal ganglia and axons demonstrable in the pedal nerves suggests that one possible function for a molluscan allatostatin-like peptide would be to modulate muscular function. Extract of Stagnicola CNS effected 50% inhibition of juvenile hormone synthesis by corpora allata of D. punctata at between 15 and 30 CNS equivalents, providing further evidence that the molluscan immunoreactive material is a peptide, or peptides, with sequence similarity to the active part of the D. punctata allatostatins.
Rudolph
P H
PH
Department of Biological Sciences, University of Iowa, Iowa City 52242, USA.
Stay
B
B
D000818:Animals; D001369:Axons / Q000378:metabolism; D002033:Bulinus / Q000378:metabolism*; D002490:Central Nervous System / Q000187:drug effects / Q000378:metabolism*; D003058:Cockroaches / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D007150:Immunohistochemistry; D007605:Juvenile Hormones / Q000096:biosynthesis; D008195:Lymnaea / Q000378:metabolism*; D009479:Neuropeptides / Q000096:biosynthesis / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology
D007605:Juvenile Hormones; D009479:Neuropeptides; D014020:Tissue Extracts; C076523:allatostatin