Genomic structure and chromosomal localization of GML (GPI-anchored molecule-like protein), a gene induced by p53.
Among its known functions, tumor suppressor gene p53 serves as a transcriptional regulator and mediates various signals through activation of downstream genes. We recently identified a novel gene, GML (glycosylphosphatidylinositol (GPI)-anchored molecule-like protein), whose expression is specifically induced by wildtype p53. To characterize the GML gene further, we determined 35.8 kb of DNA sequence that included a consensus binding sequence for p53 and the entire GML gene. The GML gene consists of four exons; and the p53-binding sequence is present in the 5'-flanking region. In genomic organization this gene resembles genes encoding murine Ly-6 glycoproteins, a human homologue of the Ly-6 family called RIG-E, and CD59; products of these genes, known as GPI-anchored proteins, are variously involved in signal transduction, cell-cell adhesion, and cell-matrix attachment. FISH analysis revealed that the GML gene is located on human chromosome 8q24.3. Genes encoding at least two other GPI-anchored molecules, E48 and RIG-E, are also located in this region.
Kimura
Y
Y
Laboratory of Molecular Medicine, University of Tokyo, Japan.
Furuhata
T
T
Urano
T
T
Hirata
K
K
Nakamura
Y
Y
Tokino
T
T
D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion / Q000235:genetics; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005091:Exons; D058851:GPI-Linked Proteins; D005786:Gene Expression Regulation; D016158:Genes, p53; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D015398:Signal Transduction / Q000235:genetics
D018797:Cell Cycle Proteins; C102519:GML protein, human; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D004247:DNA