BstAPI, an ApaBI isoschizomer, cleaves DNA at 5'-GCANNNN NTGC-3'.
Cleavage positions of Bst API, a new restriction endonuclease (ENase) that recognizes palindromic interrupted DNA sequence, have been determined. Recognition sequences and cleavage sites comparison shows that Bst API shares similarity with a number of type II restriction enzymes.
Abdurashitov
M A
MA
SibEnzyme, 8 Lavrentiev Str., Novosibirsk 630090, Russia. info@siben.nsk.su
Belichenko
O A
OA
Shevchenko
A V
AV
Dedkov
V S
VS
Degtyarev
S K
SK
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C080472:GCANNNNNTGC -specific type II deoxyribonucleases