A rapid and simple method to quantify Helicobacter pylori adhesion to human gastric MKN-28 cells.
A rapid and simple method to quantitatively analyse Helicobacter pylori adhesion to human gastric epithelial cells was developed by using an enzyme-linked immunosorbent assay. This method made it possible to quantitatively evaluate the adhesion activities of many different H. pylori strains at a time. Our studies indicate that this method is well suited for the quantitative analysis of H. pylori adhesion to gastric epithelial cells.
Hayashi
S
S
Department of Microbiology, School of Medicine, Sapporo Medical University, Hokkaido, Japan.
Sugiyama
T
T
Yachi
A
A
Yokota
K
K
Hirai
Y
Y
Oguma
K
K
Fujii
N
N
D001422:Bacterial Adhesion / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000378:metabolism*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured