Interaction of ozone exposure with airway hyperresponsiveness and inflammation induced by trimellitic anhydride in sensitized guinea pigs.
The effect of prior ozone (O3) exposure on airway hyperresponsiveness and inflammation induced by trimellitic anhydride (TMA) has been investigated in TMA-sensitized guinea pigs. Airway responsiveness was measured as the concentration of acetylcholine needed to increase baseline lung resistance (RL) by 300% (PC300). Ozone (3 ppm for 3 h) caused an increase in -log PC300 at 1 h after exposure, with return of -log PC300 to control levels at 8 h. Ozone also increased baseline RL at 8 h. TMA challenge increase -log PC300 in TMA-sensitized guinea pigs at 8 h after challenge from 3.85 +/- 0.09 to 4.11 +/- 0.09. Ozone exposure prior to TMA challenge prevented the induction of airway hyperresponsiveness with a mean -log PC300 of 3.51 +/- 0.20, which was not different from that of control TMA-sensitized group. Baseline RL was significantly higher in ozone-pretreated animals after TMA challenge when compared to those of either control or challenged with TMA alone. Ozone had no effect on TMA challenge-induced BAL eosinophilia and neutrophilia. We conclude that a single exposure to ozone inhibits the increase in airway responsiveness but increases the bronchoconstrictor response induced by TMA in TMA-sensitized guinea pigs; however, the inflammatory airway response to TMA is unchanged by preexposure to ozone.
Sun
J
J
Department of Thoracic Medicine, National Heart & Lung Institute, London, United Kingdom.
Chung
K F
KF
D000109:Acetylcholine / Q000494:pharmacology; D000403:Airway Resistance / Q000187:drug effects; D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D001991:Bronchitis / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004195:Disease Models, Animal; D004347:Drug Interactions; D004802:Eosinophilia / Q000139:chemically induced; D006168:Guinea Pigs; D007958:Leukocyte Count; D008168:Lung / Q000187:drug effects*; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D010796:Phthalic Anhydrides / Q000008:administration & dosage*
D000485:Allergens; D010083:Oxidants, Photochemical; D010796:Phthalic Anhydrides; D010126:Ozone; C015559:trimellitic anhydride; D000109:Acetylcholine