title: Sendai virus efficiently infects cells via the asialoglycoprotein receptor and requires the presence of cleaved F0 precursor proteins for this alternative route of cell entry.
abstract: Biochemical evidence suggests that the asialoglycoprotein receptor (ASGP-R) can be used as an alternative receptor for a temperature-sensitive Sendai virus (SV) mutant. We now have investigated this possible alternative route of infection for SV wild-type (SV-wt) strain Fushimi by using a pair of cell lines which differ only with regard to ASGP-R expression. Infection studies after enzymatic destruction of conventional sialic acid-containing SV receptors (SA-R) revealed that only ASGP-R-expressing cells could be infected by SV-wt. This alternative route of cell entry could be completely blocked by incubation of cells with ASGP-R-specific antibodies prior to infection. Furthermore, cleavage of SV-F0 precursor protein into the subunits F1 and F2 was necessary to establish infection via ASGP-R, suggesting a fusion-mediated cell entry after binding of SV-wt to the ASGP-R on host cells. Interestingly, infection via ASGP-R was found to be nearly as efficient as infection via conventional sialic acid-containing SV receptors. A possible physiological role of the ASGP-R-mediated route of SV infection is discussed.
authors.affiliation: Abteilung Innere Medizin I, Medizinische Universitätsklinik Tübingen,Germany. michael.bitzer@uni-tuebingen.de
authors.forename: M
authors.initials: M
authors.lastname: Bitzer
authors.forename: U
authors.initials: U
authors.lastname: Lauer
authors.forename: C
authors.initials: C
authors.lastname: Baumann
authors.forename: M
authors.initials: M
authors.lastname: Spiegel
authors.forename: M
authors.initials: M
authors.lastname: Gregor
authors.forename: W J
authors.initials: WJ
authors.lastname: Neubert
mesh_terms: D016475:3T3 Cells; D000818:Animals; D037263:Asialoglycoprotein Receptor; D002460:Cell Line; D002522:Chlorocebus aethiops; D004285:Dogs; D006023:Glycoproteins / Q000378:metabolism; D051379:Mice; D011498:Protein Precursors / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D018108:Respirovirus / Q000378:metabolism* / Q000502:physiology; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D037263:Asialoglycoprotein Receptor; C099057:F0 protein, Sendai virus; D006023:Glycoproteins; D011498:Protein Precursors; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D014759:Viral Envelope Proteins