Antibiotic resistance in Streptomyces lividans: fluorescence assay for streptogramin B lyase.
A fluorescence assay for streptogramin B lyase, an enzyme that confers resistance to streptogramin B antibiotics, has been developed. The antibiotic substrates are fluorescent and the linear peptide products formed in the lyase-catalyzed reaction are relatively nonfluorescent. The assay has potential for assessing bacterial resistance to streptogramin B antibiotics and will be utilized to direct the purification of streptogramin B lyase from bacterial extracts.
Bateman
K P
KP
Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
Armstrong
S M
SM
White
R L
RL
Ross
N W
NW
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D019753:Intramolecular Lyases; D007535:Isomerases / Q000032:analysis*; D018942:Macrolides; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013302:Streptomyces / Q000187:drug effects* / Q000201:enzymology*; D013379:Substrate Specificity; D014769:Virginiamycin / Q000494:pharmacology*; D047630:Depsipeptides
D000900:Anti-Bacterial Agents; D019753:Intramolecular Lyases; D007535:Isomerases; D018942:Macrolides; D014769:Virginiamycin; C106455:streptogramin B lyase; C004910:etamycin; D047630:Depsipeptides