A 2-Mb YAC contig and physical map of the natural killer gene complex on mouse chromosome 6.
We have constructed a physical map of a > 2-Mb region on mouse chromosome 6 that contains the natural killer gene complex (NKC). The map comprises a contig of 14 overlapping yeast artificial chromosomes onto which we positioned 25 NKC markers. NKC genetically linked genes encode > 17 proteins that directly control innate NK cell-mediated tumor lysis and disease resistance. Herein we show that Nkrp1 genes are clustered in a region flanked by A2m and Cd69 genes and that most Ly49 genes are clustered in a distal region -1 Mb distant. Importantly, syntenic intervals of mouse chromosome 6 and human chromosome 12p that include the NKC are conserved. NKC species conservation suggests that the human NKC may contain orthologues for the mouse viral disease resistance genes, Cmv1 and Rmp1. The high-resolution NKC map will facilitate investigation of NKC gene regulation and identification of phenotypically defined gene products that confer NK cell defense against viral pathogens.
Brown
M G
MG
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Fulmek
S
S
Matsumoto
K
K
Cho
R
R
Lyons
P A
PA
Levy
E R
ER
Scalzo
A A
AA
Yokoyama
W M
WM
D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D055651:NK Cell Lectin-Like Receptor Subfamily B; D010641:Phenotype; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites; D013045:Species Specificity; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D017931:DNA Primers; D005819:Genetic Markers; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D000099192:CD69 Antigens; C527203:KLRB1 protein, human