Contribution to the physically anchored linkage map of the pig.
Thirty-three microsatellites have been mapped on the PiGMaP porcine genetic map. By comparison with the previously published PiGMaP maps, the maps of chromosome 2 (140 cM/70 cM) and chromosome 3 (180 cM/110 cM) were extended and new markers were mapped on the p-arm extremity of chromosome 7 and on the centromeric extremity of chromosome 15. New orders are proposed for markers on chromosomes 3 and 17. Six microsatellites isolated from cosmids were also localized on the cytogenetic map by fluorescent in situ hybridization. We tested the subcloning ligation mixture-polymerase chain reaction (SLiM-PCR) method for isolating microsatellites from cosmids. Subcloning is more effective when the cosmid harbours several microsatellites whereas SLiM-PCR is more straightforward when the cosmid contains a single microsatellite. Fifteen anonymous microsatellites were regionally assigned by using a hybrid cell panel. For map integration, the determination of a regional assignment of anonymous microsatellites by using a hybrid cell panel offers an alternative to microsatellite isolation from cosmids and their localizations by in situ hybridization.
Robic
A
A
INRA, Laboratoire de Génétique Cellulaire, Tolosan, France.
Milan
D
D
Woloszyn
N
N
Riquet
J
J
Yerle
M
M
Nagel
M
M
Bonnet
M
M
Pinton
P
P
Dalens
M
M
Gellin
J
J
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003582:Cytogenetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013552:Swine / Q000235:genetics*
D017931:DNA Primers