Clean-up and re-use of kieselguhr (Extrelut) for liquid-liquid extraction of urinary cortisol.
Cortisol was isolated from human urine using kieselguhr (Extrelut)-filled columns. After use, Extrelut was cleaned-up once with distilled water and twice with ethanol. Before re-use, the cleaned-up kieselguhr was dried for 24 h by a warm air stream. The comparison of cortisol recovery from human urine and HPLC chromatograms of urinary extracts show that Extrelut can be repeatedly used for liquid-liquid extraction of urinary cortisol.
Fenske
M
M
Department of Animal Physiology, University of Bayreuth, NW I, Germany.
D002851:Chromatography, High Pressure Liquid; D007692:Diatomaceous Earth; D006801:Humans; D006854:Hydrocortisone / Q000652:urine*; D008297:Male
D007692:Diatomaceous Earth; C018540:Extrelut; D006854:Hydrocortisone