[Study of hepatitis E virus replication in FRhK-4 cell culture].
The cytolytic form of hepatitis E virus reproduction in FRhk-4 cells is described. The cytopathic effect was observed by days 6-7 of the 25th passage in this cell culture. Reverse transcription with polymerase chain reaction were used to study virus reproduction daily for 10 days postinfection. The virus replication starts on day 4 after inoculation. The development of the cytopathic effect depended on the serum concentration in culture medium, whereas the virus replication did not depend on this factor. The replicative form (-RNA) of viral RNA appeared on day 4 postinoculation, as did the new genomic one (+RNA).
Dzagurov
G K
GK
Kupriianov
V V
VV
Balaian
M S
MS
D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D016679:Genome, Viral; D016752:Hepatitis E virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D008253:Macaca mulatta; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D014779:Virus Replication
D012367:RNA, Viral