title: Direct analysis of fluoxetine and norfluoxetine in plasma by gas chromatography with nitrogen-phosphorus detection.
abstract: A quantitative method for the simultaneous GC resolution and detection of fluoxetine and his metabolite norfluoxetine in human plasma was developed. The procedure required 1.0 ml of plasma, extraction with a mixed organic solvent and injection into a capillary gas chromatograph with an OV-1 fused-silica column coupled to a nitrogen-phosphorus detector. The calibration curves were linear over the range 5-3000 ng/ml. The detection limits were 0.3 and 2 ng/ml for fluoxetine and norfluoxetine, respectively. The assay is suitable for routine analysis.
authors.affiliation: Laboratoire de Pharmacologie, Centre Hospitalier Universitaire de Grenoble, France.
authors.forename: P
authors.initials: P
authors.lastname: Fontanille
authors.forename: N
authors.initials: N
authors.lastname: Jourdil
authors.forename: C
authors.initials: C
authors.lastname: Villier
authors.forename: G
authors.initials: G
authors.lastname: Bessard
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D005473:Fluoxetine / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D009584:Nitrogen; D010758:Phosphorus; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005473:Fluoxetine; D010758:Phosphorus; C036139:norfluoxetine; D009584:Nitrogen