Identification of an ATPase activity associated with a 71-kilodalton polypeptide encoded in gene 1 of the human coronavirus 229E.
Human coronavirus 229E gene expression involves proteolytic processing of the gene 1-encoded polyproteins pp1a and pp1ab. In this study, we have detected a 71-kDa polypeptide in virus-infected cells that is released from pp1ab by the virus-encoded 3C-like proteinase and that has been predicted to contain both metal-binding and helicase domains. The polypeptide encompasses amino acids Ala-4996 to Gln-5592 of pp1ab and exhibits nucleic acid-stimulated ATPase activity when expressed as a fusion protein with the Escherichia coli maltose-binding protein. These data provide the first identification of a coronavirus open reading frame 1b-encoded enzymatic activity.
Heusipp
G
G
Institute of Virology, University of Würzburg, Germany.
Harms
U
U
Siddell
S G
SG
Ziebuhr
J
J
D000086042:3C Viral Proteases; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D002460:Cell Line; D017934:Coronavirus / Q000201:enzymology* / Q000235:genetics; D028941:Coronavirus 229E, Human; D003546:Cysteine Endopeptidases / Q000378:metabolism; D006801:Humans; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D010455:Peptides; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C087637:gene 1 protein, Coronavirus; D000255:Adenosine Triphosphate; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases; D000251:Adenosine Triphosphatases