title: Cell cycle kinetic studies in 68 patients with myelodysplastic syndromes following intravenous iodo- and/or bromodeoxyuridine.
abstract: Sixty-eight patients with myelodysplastic syndromes (MDS) received sequential infusions of iodo- and/or bromodeoxyuridine for cell kinetic analysis. Bone marrow biopsy sections were treated by appropriate antibodies and a labeling index (LI), duration of S-phase (Ts), and total cell cycle time (Tc) of myeloid cells were determined. The mean LI was 28.4%, Ts was 11.8 hours and Tc was 40.7 hours. The %LI decreased as the disease evolved from refractory anemia toward transformation to acute leukemia (p = 0.04). Double-labeling of biopsy sections for apoptosis and proliferation showed that 30-90% of S-phase cells in MDS patients were simultaneously apoptotic or "antonymous." We conclude that MDS are highly proliferative disorders in which the ineffective hematopoiesis is probably the result of excessive apoptosis rather than slow proliferation.
authors.affiliation: Rush Cancer Institute, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612-3824, USA.
authors.forename: A
authors.initials: A
authors.lastname: Raza
authors.forename: S
authors.initials: S
authors.lastname: Alvi
authors.forename: L
authors.initials: L
authors.lastname: Broady-Robinson
authors.forename: M
authors.initials: M
authors.lastname: Showel
authors.forename: J
authors.initials: J
authors.lastname: Cartlidge
authors.forename: S D
authors.initials: SD
authors.lastname: Mundle
authors.forename: V T
authors.initials: VT
authors.lastname: Shetty
authors.forename: R Z
authors.initials: RZ
authors.lastname: Borok
authors.forename: S E
authors.initials: SE
authors.lastname: Dar
authors.forename: H K
authors.initials: HK
authors.lastname: Chopra
authors.forename: L
authors.initials: L
authors.lastname: Span
authors.forename: A
authors.initials: A
authors.lastname: Parcharidou
authors.forename: C
authors.initials: C
authors.lastname: Hines
authors.forename: S
authors.initials: S
authors.lastname: Gezer
authors.forename: P
authors.initials: P
authors.lastname: Venugopal
authors.forename: J
authors.initials: J
authors.lastname: Loew
authors.forename: J
authors.initials: J
authors.lastname: Showel
authors.forename: D
authors.initials: D
authors.lastname: Alston
authors.forename: B
authors.initials: B
authors.lastname: Hernandez
authors.forename: S
authors.initials: S
authors.lastname: Rifkin
authors.forename: E
authors.initials: E
authors.lastname: Robin
authors.forename: R
authors.initials: R
authors.lastname: Shah
authors.forename: S A
authors.initials: SA
authors.lastname: Gregory
mesh_terms: D017209:Apoptosis; D001973:Bromodeoxyuridine; D002453:Cell Cycle; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007065:Idoxuridine; D009190:Myelodysplastic Syndromes / Q000473:pathology*
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine; D007065:Idoxuridine