Induction of c-fos mRNA by lead in PC 12 cells.
Addition of lead acetate to PC 12 pheochromocytoma cells elicits induction of c-fos, an immediate early response gene. Induction of c-fos was concentration- and time-dependent: the lowest concentration of lead acetate tested that induced c-fos was 10 microM; induction was observed after a 30 min incubation and remained high after 90 min. Treatment with lead acetate and cycloheximide superinduced c-fos mRNA. Actinomycin D, an inhibitor of mRNA transcription, decreased the level of c-fos mRNA induced by lead acetate by almost 80%. Cadmium chloride and zinc chloride did not induce c-fos mRNA. Since the c-fos gene encodes a transcription factor, Pb2+ has the potential to deregulate the expression of other genes.
Kim
K
K
Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Annadata
M
M
Goldstein
G W
GW
Bressler
J P
JP
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005786:Gene Expression Regulation; D016762:Genes, fos; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D009942:Organometallic Compounds / Q000494:pharmacology*; D009994:Osmolar Concentration; D016716:PC12 Cells / Q000187:drug effects* / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D019384:Nucleic Acid Synthesis Inhibitors; D009942:Organometallic Compounds; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D003609:Dactinomycin; D003513:Cycloheximide; C008261:lead acetate