title: Separation of 18 6-aminoquinolyl-carbamyl-amino acids by ion-pair chromatography.
abstract: Eighteen 6-aminoquinolyl-carbamyl (AQC)-amino acids were separated by ion-pair chromatography, using tetrabutylammonium (TBA) as a counterion. Optimum separation was obtained on a C8 reverse-phase column using gradient elution with two mobile phases, A (5 mM TBA, 75 mM ammonium acetate, pH 7.5) and B (80% acetonitrile). The AQC-amino acids were detected by fluorescence with excitation at 250 nm and emission at 395 nm, and the analysis time was 65 min. The response factors of individual AQC-amino acids to AQC-phenylalanine ranged from 0.42 to 1.08 (except for tryptophan at 0.01), with an average of 0.8. Detection limits by fluorescence ranged from 11.8 fmol (threonine) to 51.7 fmol (methionine), except for tryptophan (1.8 pmol).
authors.affiliation: Division of Biochemical Analysis, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shindo
authors.forename: S
authors.initials: S
authors.lastname: Nojima
authors.forename: T
authors.initials: T
authors.lastname: Fujimura
authors.forename: H
authors.initials: H
authors.lastname: Taka
authors.forename: R
authors.initials: R
authors.lastname: Mineki
authors.forename: K
authors.initials: K
authors.lastname: Murayama
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification*; D000634:Aminoquinolines / Q000737:chemistry*; D002138:Calibration; D002264:Carboxylic Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002919:Chymotrypsinogen / Q000032:analysis; D003545:Cysteine / Q000032:analysis; D006868:Hydrolysis; D012995:Solubility; D014364:Tryptophan / Q000032:analysis; D014867:Water / Q000737:chemistry
chemical_list: D000596:Amino Acids; D000634:Aminoquinolines; D002264:Carboxylic Acids; D014867:Water; D014364:Tryptophan; D002919:Chymotrypsinogen; D003545:Cysteine