Increased endothelial cell retraction and tumor cell invasion by soluble factors derived from pancreatic cancer cells.
BACKGROUND
Tumor cells induce endothelial cell retraction before invasion. In pancreatic cancer cells, the factors affecting endothelial cell retraction are not well-understood.


METHODS
The activities of the endothelial cell retraction in conditioned media (CM) derived from three human pancreatic cancer cell lines, PSN-1, MiaPaca-2, and Capan-1, were measured for the amount of intercellular junctional transport of FITC dextran through an endothelial cell monolayer in a transwell cell culture system.


RESULTS
The CM derived from the three pancreatic cancer cells induced endothelial cell retraction. The endothelial cell retraction activity in the CM from PSN-1 cells was significantly higher than those from MiaPaca-2 and Capan-1 cells. The CM from PSN-1 cells enhanced both the adhesion and the invasion of MiaPaca-2 and Capan-1 cells. The factors with endothelial cell retraction activity in the CM from PSN-1 cells were characterized as heat-stable, trypsin-sensitive glycoproteins ranging from 10,000 to 50,000 in molecular weight, and were found both in heparin-bound and unbound fractions.


CONCLUSIONS
PSN-1 cells produced and secreted at least two factors inducing the endothelial cell retraction. The factors could play an important role in the establishment of invasion and metastasis of PSN-1 cells.
Nakamori
S
S
Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.
Okamoto
H
H
Kusama
T
T
Shinkai
K
K
Mukai
M
M
Ohigashi
H
H
Ishikawa
O
O
Furukawa
H
H
Imaoka
S
S
Akedo
H
H
D002448:Cell Adhesion; D005591:Chemical Fractionation; D017077:Culture Media, Conditioned; D004727:Endothelium / Q000378:metabolism / Q000473:pathology; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D008970:Molecular Weight; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate