title: The protein composition of normal and developmentally defective enamel.
abstract: The development of human enamel involves a complex series of events including the secretion and degradation of a unique extracellular matrix. Ameloblasts progress through a succession of cellular phenotypes executing specialized secretory and regulatory functions. When performing optimally, ameloblasts produce a highly structured and mineralized tissue. Given the elaborate developmental events required for normal enamel formation, it is not surprising that a variety of enamel malformations arise from defects in matrix synthesis, secretion and extracellular processing. Normal matrix secretion and post-secretory processing by ameloblasts can be affected by a variety of hereditary and environmental conditions. These disturbances can result in an abnormal amount and/or composition of matrix proteins, and subsequently, an altered enamel structure and/or mineral content. For example, abnormal matrix removal during enamel maturation apparently contributes to hypomineralization associated with dental fluorosis. Incomplete matrix removal can also occur in several different forms of the hereditary condition amelogenesis imperfects. Specific types of this condition can have retention of substantial enamel protein (e.g. 5% by weight) that is, at least in part, composed of amelogenin and/or its breakdown products. Characterization of the enamel proteins in teeth affected by developmental disturbances can provide insight into the pathogenesis and normal formation of this highly specialized tissue.
authors.affiliation: Department of Pediatric Dentistry, University of North Carolina School of Dentistry, Chapel Hill 27599-7450, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Wright
authors.forename: K
authors.initials: K
authors.lastname: Hall
authors.forename: M
authors.initials: M
authors.lastname: Yamauchi
mesh_terms: D000328:Adult; D000565:Ameloblasts / Q000378:metabolism; D000566:Amelogenesis / Q000502:physiology; D000567:Amelogenesis Imperfecta / Q000378:metabolism*; D053523:Amelogenin; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D002648:Child; D003744:Dental Enamel Hypoplasia / Q000378:metabolism; D003746:Dental Enamel Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019229:Dentition, Permanent; D004591:Electrophoresis, Polyacrylamide Gel; D009050:Fluorosis, Dental / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D051381:Rats; D014094:Tooth, Deciduous
chemical_list: D053523:Amelogenin; C507201:Amelx protein, rat; D000596:Amino Acids; D003746:Dental Enamel Proteins; C038456:tuftelin