title: Chromosome assignment of 115 expressed sequence tags (ESTs) from human skeletal muscle.
abstract: The chromosome assignment of 115 expressed sequence tags (ESTs) from human skeletal muscle, 101 of which identify unknown human genes, is reported. The ESTs were selected among over 4,000 obtained from systematic sequencing of a skeletal muscle cDNA library containing 3' portions of the mRNAs. Chromosome assignments were obtained by PCR amplification of two panels of human x rodent somatic cell hybrids. Analysis of these preliminary data suggests a nonrandom distribution of muscle ESTs in the human chromosome complement. The unexpected occurrence of multiple chromosome localizations for some ESTs is discussed.
authors.affiliation: CRIBI Biotechnology Centre, Università degli Studi di Padova, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Muraro
authors.forename: D
authors.initials: D
authors.lastname: Stephan
authors.forename: N
authors.initials: N
authors.lastname: Tiso
authors.forename: R
authors.initials: R
authors.lastname: Zimbello
authors.forename: G A
authors.initials: GA
authors.lastname: Danieli
authors.forename: E H
authors.initials: EH
authors.lastname: Hoffman
authors.forename: G
authors.initials: G
authors.lastname: Valle
authors.forename: G
authors.initials: G
authors.lastname: Lanfranchi
mesh_terms: D000328:Adult; D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002875:Chromosomes; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D018482:Muscle, Skeletal / Q000378:metabolism*; D012377:Rodentia
chemical_list: D018076:DNA, Complementary