title: The protein cofactor allows the sequence of an RNase P ribozyme to diversify by maintaining the catalytically active structure of the enzyme.
abstract: To study the effect proteins have on the catalysis and evolution of RNA enzymes, we simulated evolution of RNase P catalytic M1 RNA in vitro, in the presence and absence of its C5 protein cofactor. In the presence of C5, functional M1 sequence variants (not catalytically active in the absence of C5) were selected in addition to those identical to M1. C5 maintains the catalytically active structure of the variants and allows for an enhanced spectrum of M1 molecules to function in the context of a ribonucleoprotein (RNP) complex. The generation of an RNP enzyme, requiring both RNA and protein components, from a catalytically active RNA molecule has implications for how modern RNP complexes evolved from ancestral RNAs.
authors.affiliation: School of Public Health, University of California, Berkeley 94720, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Kim
authors.forename: A F
authors.initials: AF
authors.lastname: Kilani
authors.forename: X
authors.initials: X
authors.lastname: Zhan
authors.forename: S
authors.initials: S
authors.lastname: Altman
authors.forename: F
authors.initials: F
authors.lastname: Liu
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D017124:Conserved Sequence; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D019143:Evolution, Molecular; D014644:Genetic Variation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism; D012365:RNA, Transfer, Tyr / Q000378:metabolism; D043262:Ribonuclease P; D012261:Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012322:RNA Precursors; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D012365:RNA, Transfer, Tyr; D012261:Ribonucleoproteins; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli