title: Cellular distribution of myosin-V in the guinea pig cochlea.
abstract: The importance of unconventional myosins to hearing has recently been revealed by the identification of myosins-VI and -VII as the defective genes in mouse mutations and in a human syndrome which lead to profound hearing loss. Another class of novel myosins (V) has been implicated in the trafficking of intracellular vesicles in neurons and other secretory cells. We used affinity-purified antibodies to determine the localization of myosin-V in the guinea pig inner ear. In the sensory epithelium of the cochlea, myosin-V epitopes were recognized in neuronal and supporting cells. Neuronal labelling was most intense in the afferent innervation of inner and outer hair cells. Supporting cells labelled were cells of Hensen and Deiters, and inner border, inner phalangeal, inner sulcus and interdental cells. In the vascular tissue of the cochlea, we observed staining of intermediate cells of the stria vascularis and of border cells between the stria and the spiral prominence. Staining of afferent chalice nerve endings was observed on type I vestibular hair cells. The results suggest that, like myosins VI and VII, myosin-V is localized in positions that may be critical to auditory function.
authors.affiliation: Laboratory of Cellular Biology, NIDCD-NIH, Rockville, MD 20850-3320, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Coling
authors.forename: E M
authors.initials: EM
authors.lastname: Espreafico
authors.forename: B
authors.initials: B
authors.lastname: Kachar
mesh_terms: D000818:Animals; D001306:Auditory Pathways / Q000166:cytology; D015153:Blotting, Western; D002148:Calmodulin-Binding Proteins / Q000032:analysis*; D003051:Cochlea / Q000737:chemistry* / Q000166:cytology* / Q000294:innervation; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000166:cytology; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D018994:Myosin Light Chains / Q000032:analysis; D024701:Myosin Type V; D009412:Nerve Fibers / Q000648:ultrastructure*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D013136:Spiral Ganglion / Q000166:cytology
chemical_list: D002148:Calmodulin-Binding Proteins; D018994:Myosin Light Chains; D009419:Nerve Tissue Proteins; C078829:chicken brain myosin-V p190; D024701:Myosin Type V