title: Lack of effect of granulocyte-macrophage colony-stimulating factor on secretion of interferon-tau, other proteins, and prostaglandin E2 by the bovine and ovine conceptus.
abstract: Three experiments tested the effects of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) on the preimplantation bovine and ovine conceptus. There was no effect of rbGM-CSF on the secretion of total radiolabeled protein in conditioned medium, immunoreactive interferon-tau (IFN tau), antiviral activity, or prostaglandin E2 from Day 16-18 bovine conceptuses cultured for 24 hr with, [3H]leucine and +/- 10 ng/ml rbGM-CSF. Similarly, there was no effect of 1 ng/ml rbGM-CSF on the secretion of total radiolabeled protein. IFN tau, or antiviral activity from Day 17 ovine conceptuses. There was also no beneficial effect of 1 or 10 ng/ml rbGM-CSF on the presence of immunoreactive IFN tau in conditioned medium from in vitro-produced bovine blastocysts at Day 7-8 after fertilization. Results indicate that IFN tau secretion from bovine and ovine conceptuses are unresponsive to rbGM-CSF at the concentrations tested.
authors.affiliation: Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611-0920, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: de Moraes
authors.forename: J A
authors.initials: JA
authors.lastname: Davidson
authors.forename: J G
authors.initials: JG
authors.lastname: Fleming
authors.forename: F W
authors.initials: FW
authors.lastname: Bazer
authors.forename: J L
authors.initials: JL
authors.lastname: Edwards
authors.forename: J G
authors.initials: JG
authors.lastname: Betts
authors.forename: P J
authors.initials: PJ
authors.lastname: Hansen
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D017077:Culture Media, Conditioned; D015232:Dinoprostone / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D007315:Insemination, Artificial; D007370:Interferon Type I; D007371:Interferon-gamma / Q000378:metabolism*; D009924:Organ Culture Techniques; D011257:Pregnancy Proteins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine
chemical_list: D017077:Culture Media, Conditioned; D007370:Interferon Type I; D011257:Pregnancy Proteins; D011506:Proteins; D011994:Recombinant Proteins; C020947:interferon tau; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015232:Dinoprostone