Cytotoxic T lymphocyte control during ectromelia (mousepox) virus infection: interaction between MHC-restricted cells analyzed by non-radioactive fluorometry.
Cytotoxic T lymphocyte (CTL) activity of draining lymph node (DLN) cells isolated from BALB/c mice infected with ectromelia virus (EV) was examined using a fluorometric cell-mediated cytotoxicity (CMC) assay. Specific lysis of target cells A20 and EMT-6 primed with EV was demonstrated. The classical CD8+ cytolytic pathway dominated (72.7%) as compared to that of CD4+ (27.3%) in the cellular response during acute EV infection. Also an alternative method for determining CMC, employing a bisbenzamide dye for labelling target cells, is described. Coefficient variations of relative fluorescence were below 6%, that makes the method sensitive and reliable.
Toka
F N
FN
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Warsaw Agricultural University, Poland.
Niemiałtowski
M G
MG
Spohr de Faundez
I
I
Gieryńska
M
M
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D004482:Ectromelia, Infectious / Q000276:immunology*; D005470:Fluorometry; D007553:Isotope Labeling; D018655:Lymphocyte Count; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured