Determination of genotypes of hepatitis C virus in Venezuela by restriction fragment length polymorphism.
Hepatitis C virus genotypes in Venezuela were analyzed by restriction fragment length polymorphism in the 5' noncoding region. The absence of BstUI digestion was found to be a useful marker for genotype 2 specimens. From 122 serum samples, 66, 20, and 2.5% were classified as genotypes 1, 2, and 3, respectively; 0.8% were classified as genotype 4; and 10% appeared to be mixed infections.
Pujol
F H
FH
Laboratorio Biología de Virus, CMBC, IVIC, Caracas, Venezuela. fpujol@pasteur.ivic.ve
Loureiro
C L
CL
Devesa
M
M
Blitz
L
L
Parra
K
K
Beker
S
S
Liprandi
F
F
D005814:Genes, Viral / Q000235:genetics; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D014687:Venezuela / Q000453:epidemiology