Identification of ovine herpesvirus-2 infection in sheep.
A polymerase chain reaction test for the detection of ovine herpesvirus-2 (OHV-2) DNA was used to identify sites of OHV-2 infection in peri-natal lambs and in adult sheep. OHV-2 was detected in the nasal secretions from all lambs within a period of two months following birth. Subsequently, OHV-2 DNA was identified in a number of epithelial tissues including the cornea, turbinates and pharynx. In addition, OHV-2 DNA was detected exclusively in B-lymphocytes from six of ten adult sheep tested. An infection cycle for OHV-2 in sheep is proposed which bears similarities with the gammaherpesviruses Epstein-Barr virus and mouse herpesvirus-68.
Baxter
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SI
Moredun Research Institute, Edinburgh, U.K.
Wiyono
A
A
Pow
I
I
Reid
H W
HW
D000818:Animals; D001402:B-Lymphocytes / Q000821:virology; D004279:DNA, Viral; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis* / Q000662:veterinary / Q000821:virology; D009297:Nasal Mucosa / Q000378:metabolism / Q000821:virology; D009693:Nucleic Acid Hybridization; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000821:virology
D004279:DNA, Viral