title: [Intervention of Campylobacter jejuni subsp. jejuni flagella in the adhesion to cellular cultures: bacteriological and immunological evidence].
abstract: The participation of the flagella of a virulent strain (O52) of Campylobacter jejuni subsp. jejuni in the adhesion to HEp-2 cells and their inhibition by means of homologous polyclonal antibodies, monoclonal anti-flagella antibodies and colostral natural antibodies (IgA) was studied. An aflagellated strain (T1) was used as negative control. Adhesion was observed in higher rates with O52 strain (72%) than with T1 strain (27.5%). Polyclonal, monoclonal and colostral antibodies inhibited O52 strain adhesion in more than 70% (p < 0.001). T1 strain adhesion was inhibited only by polyclonal and colostral natural antibodies. Our results suggest that the flagella of C. jejuni subsp. jejuni could participate effectively in the adhesion process. However, the inhibition of T1 strain by polyclonal and colostral antibodies suggests the existence of other binds of adhesins in the bacterial surface.
authors.affiliation: Institutos de Microbiología Clínica, Inmunología y Parasitología, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: H
authors.initials: H
authors.lastname: Fernández
authors.forename: H
authors.initials: H
authors.lastname: Folch
authors.forename: G
authors.initials: G
authors.lastname: Eller
authors.forename: N
authors.initials: N
authors.lastname: Navarrete
authors.forename: E
authors.initials: E
authors.lastname: Andrews
authors.forename: T
authors.initials: T
authors.lastname: Gajardo
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D016123:Campylobacter jejuni / Q000276:immunology / Q000502:physiology*; D002478:Cells, Cultured; D003126:Colostrum / Q000276:immunology; D005260:Female; D005407:Flagella / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007223:Infant; D008297:Male; D011247:Pregnancy