title: Protein purification and nucleotide sequence of a lysozyme from the bacteria-induced larvae of the fall webworm, Hyphantria cunea.
abstract: A protein with lytic activity against Micrococcus luteus was purified from the hemolymph of the fall webworm, Hyphantria cunea, larvae challenged with live E. coli. A bacteriolytic protein of about 14,000 daltons in mass was purified by cation exchange chromatography and reverse-phased HPLC. The optimum pH and optimum temperature range for activity were around pH 6.2 and 50 degrees C, respectively, in a 100 mM phosphate buffer. The amino-terminal amino acid sequence of this protein was determined and the corresponding cDNA was isolated and analyzed. The deduced protein of 142 amino acid residues was composed of a putative leader sequence of 20 residues and the mature enzyme of 122 residues. The cloned lysozyme gene was strongly induced in response to bacterial injection, implying that the enzyme is a part of the immune response of H. cunea. Comparison with other known lysozyme sequences shows that our lysozyme belongs to the chicken lysozyme.
authors.affiliation: Insect Resources Lab., Korea Research Institute of Bioscience and Biotechnology, KIST, Yusong, Taejon, Korea.
authors.forename: H Y
authors.initials: HY
authors.lastname: Park
authors.forename: S S
authors.initials: SS
authors.lastname: Park
authors.forename: S W
authors.initials: SW
authors.lastname: Shin
authors.forename: D S
authors.initials: DS
authors.lastname: Park
authors.forename: M G
authors.initials: MG
authors.lastname: Kim
authors.forename: H W
authors.initials: HW
authors.lastname: Oh
authors.forename: C K
authors.initials: CK
authors.lastname: Joo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli; D007814:Larva; D008969:Molecular Sequence Data; D009036:Moths / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D009113:Muramidase / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D009113:Muramidase