A simple method for the purification of type 1 RIPs.
We describe a straightforward and simple method for obtaining pure and active preparations of type 1 ribosome inactivating proteins (RIPs). The very high isoelectric point values, characteristic of these proteins, allow this purification in a single chromatographic step.
Marcozzi
G
G
Department of Basic and Applied Biology, University of L'Aquila, Italy.
Spano
L
L
D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007526:Isoelectric Point; D009699:N-Glycosyl Hydrolases / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry / Q000201:enzymology*; D054790:Ribosome Inactivating Proteins, Type 2; D012639:Seeds / Q000737:chemistry / Q000201:enzymology*; D016622:Silver Staining
D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D009699:N-Glycosyl Hydrolases