title: The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
abstract: Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-consuming process. In an effort to overcome these problems, several groups have successfully demonstrated the viability of the whole-genome random 'shotgun' method in large-scale sequencing of both viruses and prokaryotes. Here we report the sequence of Saccharomyces cerevisiae chromosome IX, determined in part by a whole-chromosome 'shotgun', and describe the particular difficulties encountered in the random 'shotgun' sequencing of an entire eukaryotic chromosome. Analysis of this sequence shows that chromosome IX contains 221 open reading frames (ORFs), of which approximately 30% have been sequenced previously. This chromosome shows features typical of a small Saccharomyces cerevisiae chromosome.
authors.affiliation: The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: C
authors.initials: C
authors.lastname: Churcher
authors.forename: S
authors.initials: S
authors.lastname: Bowman
authors.forename: K
authors.initials: K
authors.lastname: Badcock
authors.forename: A
authors.initials: A
authors.lastname: Bankier
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: T
authors.initials: T
authors.lastname: Chillingworth
authors.forename: R
authors.initials: R
authors.lastname: Connor
authors.forename: K
authors.initials: K
authors.lastname: Devlin
authors.forename: S
authors.initials: S
authors.lastname: Gentles
authors.forename: N
authors.initials: N
authors.lastname: Hamlin
authors.forename: D
authors.initials: D
authors.lastname: Harris
authors.forename: T
authors.initials: T
authors.lastname: Horsnell
authors.forename: S
authors.initials: S
authors.lastname: Hunt
authors.forename: K
authors.initials: K
authors.lastname: Jagels
authors.forename: M
authors.initials: M
authors.lastname: Jones
authors.forename: G
authors.initials: G
authors.lastname: Lye
authors.forename: S
authors.initials: S
authors.lastname: Moule
authors.forename: C
authors.initials: C
authors.lastname: Odell
authors.forename: D
authors.initials: D
authors.lastname: Pearson
authors.forename: M
authors.initials: M
authors.lastname: Rajandream
authors.forename: P
authors.initials: P
authors.lastname: Rice
authors.forename: N
authors.initials: N
authors.lastname: Rowley
authors.forename: J
authors.initials: J
authors.lastname: Skelton
authors.forename: V
authors.initials: V
authors.lastname: Smith
authors.forename: B
authors.initials: B
authors.lastname: Barrell
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D015825:Chromosomes, Fungal; D004271:DNA, Fungal; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004271:DNA, Fungal