Deletion in the Z-line region of the titin gene in a baby hamster kidney cell line, BHK-21-Bi.
The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titin non-functional. These sequences were present in the original BHK-21 line and in hamster DNAs. Our finding shows that, due to the absence of selective pressure on a gene's function, genomic deterioration can occur in a permanent cell line and can lead to a loss of overlapping DNA stretches in both autosomes.
Jäckel
M
M
Developmental Biology Unit, University of Bielefeld, Germany.
Witt
C
C
Antonova
O
O
Curdt
I
I
Labeit
S
S
Jockusch
H
H
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D064211:Connectin; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007668:Kidney; D008647:Mesocricetus; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion / Q000235:genetics*
D064211:Connectin; D018076:DNA, Complementary; D009124:Muscle Proteins; C577285:TTN protein, human; D011494:Protein Kinases; D015246:Deoxyribonuclease EcoRI