title: Pathophysiology of neonatal lung injury induced by monoclonal antibody to surfactant protein B.
abstract: Near-term newborn rabbits were exposed via the airways to a monoclonal antibody to surfactant protein B and ventilated for 0-120 min. Control animals received nonspecific rabbit or mouse immunoglobulin G, saline, or no material via the airways. Administration of the antibody at > or = 40 mg/kg elicited an immediate, significant fall in lung-thorax compliance associated with progressive intra-alveolar edema and/or alveolar collapse and necrosis and desquamation of airway epithelium, and hyaline membranes. The vascular-to-alveolar leak of human albumin and human immunoglobulin G, injected intravenously at birth and determined in lung lavage fluid 60-120 min after instillation of the antibody, was 1.8% for the left lung, with no difference between the markers. The average leak in control animals ventilated for 120 min was < 0.3% (P < 0.05). Cytospin preparations of lung lavage fluid from animals exposed to the antibody showed significantly increased recruitment of neutrophilic granulocytes. The pathology and pathophysiology of neonatal lung injury induced by the monoclonal antibody to surfactant protein B probably reflect a combination of direct inactivation of surfactant and an inflammatory response triggered by the immune reaction.
authors.affiliation: Division for Experimental Perinatal Pathology, Karolinska Hospital, Stockholm, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Grossmann
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: B
authors.initials: B
authors.lastname: Robertson
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: P
authors.initials: P
authors.lastname: Berggren
authors.forename: W Z
authors.initials: WZ
authors.lastname: Li
authors.forename: G W
authors.initials: GW
authors.lastname: Song
authors.forename: B
authors.initials: B
authors.lastname: Sun
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002199:Capillary Permeability; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008170:Lung Compliance; D008171:Lung Diseases / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D008854:Microscopy, Electron; D009504:Neutrophils / Q000473:pathology; D011510:Proteolipids / Q000737:chemistry / Q000276:immunology*; D011650:Pulmonary Alveoli / Q000378:metabolism; D011652:Pulmonary Circulation; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012121:Respiration, Artificial; D012709:Serum Albumin / Q000378:metabolism; D013500:Surface Tension; D007507:Therapeutic Irrigation
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011510:Proteolipids; D011663:Pulmonary Surfactants; D012709:Serum Albumin