Expression of gelatinases within the trabecular bone compartment of ovariectomized and parathyroidectomized adult female rats.
Ovariectomized (ovx) and parathyroidectomized (ptx) rat models of disturbed bone metabolism have been widely used in evaluating bone changes resulting from hormonal depletion, and are characterized by elevated and depressed bone turnover, respectively. We report here the expression of gelatinases extracted from native trabecular bone in these models. Nine-month-old female Sprague-Dawley rats were sacrificed after 3 weeks following ovx or 10 days post ptx to determine the influence of these procedures on the levels of proximal tibial bone tissue gelatinases. Identification and quantitation of these enzymes were performed via gelatin gel zymography of native tissue extracts and laser densitometry of developed gels, respectively. In the ptx model, a reduction in tissue levels of pro- and active-MMP-2 and a 45 kDa activated fragment was seen, whereas ovx exhibited significant increases in these enzymes. The MMPs are therefore clearly under the influence of factors known to modulate bone remodeling in vivo. The study of MMP levels directly extracted from bone using these experimental models may assist in developing management regimes for metabolic bone diseases through the use of drugs aimed at controlling turnover.
Mansell
J P
JP
Collagen Research, Division of Molecular and Cellular Biology, University of Bristol, Langford, UK.
Tarlton
J F
JF
Bailey
A J
AJ
D000818:Animals; D015415:Biomarkers; D001857:Bone Matrix / Q000378:metabolism; D005260:Female; D018093:Gelatinases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000378:metabolism; D010052:Ovariectomy; D016105:Parathyroidectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000737:chemistry / Q000201:enzymology*
D015415:Biomarkers; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2