Selectivity optimization for the separation of chlorophenols in an irregularly shaped experimental region in capillary electrophoresis.
The separation of seventeen chlorophenol congeners and phenol was studied as a function of several variables. The pH and the concentration of sodium dodecylsulphate (SDS) were found to be important. During the implementation of a central composite design for the optimization of the separation it appeared that a part of the domain was not feasible as it resulted in very long migration times and extremely deformed peaks. Therefore, a D-optimal design was selected within the boundaries of the feasible region. The optimization of the selectivity did not result in selective regions for a simultaneous separation. It was, however, possible to find a region for the simultaneous separation of 15 compounds. Further optimization at these optimal conditions resulted in a separation where 17 peaks could be observed.
Jimidar
M
M
Department of Pharmaceutical Biomedical Analysis (FABI), Vrije Universiteit Brussel, Belgium.
de Aguiar
P F
PF
Pintelon
S
S
Massart
D L
DL
D002733:Chlorophenols / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary; D005240:Feasibility Studies; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019800:Phenol; D010636:Phenols / Q000302:isolation & purification*; D012044:Regression Analysis; D012967:Sodium Dodecyl Sulfate / Q000032:analysis; D013499:Surface Properties
D002733:Chlorophenols; D010636:Phenols; D019800:Phenol; D012967:Sodium Dodecyl Sulfate