Development of a quantitative sampling method for periapical exudates from human root canals.
A quantitative method for collection of periapical exudates on endodontic paper points is described. In a pilot study, it was revealed that the relationship between fluid volume and wetted length of paper points had a highly significant curvilinear relationship (p < 0.0001), and a highly significant positive linear relationship was found to describe eluted and absorbed interleukin (IL)-1 beta activities from paper points. Periapical exudates from 29 root canals with apical periodontitis were collected using this method, and IL-1 beta activities in clinical specimens were measured. Periapical exudates (0.15 to 26.7 microliters) were recovered, and the range of IL-1 beta concentration was 0.1 to 179.5 ng/ml. These results showed that this sampling method was useful to analyze immunological changes in periapical lesions.
Shimauchi
H
H
Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, Japan.
Miki
Y
Y
Takayama
S
S
Imai
T
T
Okada
H
H
D003786:Dental Pulp Cavity / Q000276:immunology*; D005122:Exudates and Transudates / Q000737:chemistry / Q000276:immunology; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis; D007375:Interleukin-1 / Q000032:analysis*; D010209:Paper; D010485:Periapical Periodontitis / Q000175:diagnosis / Q000276:immunology*; D010865:Pilot Projects; D013048:Specimen Handling; D018709:Statistics, Nonparametric
D018836:Inflammation Mediators; D007375:Interleukin-1