Association of hnRNP S1 proteins C2 and D2 with vimentin intermediate filaments.
S1 proteins A-D are hnRNP proteins which were originally isolated from nuclei of various tissues, by selective extraction of pH 4.9 from the supernatants of nuclei mildly treated with DNase I or RNase A. In the present study, a hybridoma was isolated which produced a monoclonal antibody that reacted specifically with S1 proteins C2 and D2. When the antibody was used in indirect immunofluorescence staining of cultured cells, it stained, in addition to the nuclei, the cytoskeleton-like fibrous structures in the cytoplasm. We demonstrate that the cytoskeletal filaments are vimentin intermediate filaments. This is the first report on the hnRNP protein-association with cytoskeleton, and will help to clarify cytoplasmic mRNA localization as well as cytoplasmic distribution of hnRNP proteins.
Tsugawa
K
K
Department of Natural Science, Osaka Women's University, Sakai, Japan.
Takahashi
K P
KP
Watanabe
T
T
Mai
A
A
Fujimoto
D
D
Inoue
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D019084:Fluorescent Antibody Technique, Indirect; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006825:Hybridomas; D007382:Intermediate Filaments / Q000378:metabolism*; D051381:Rats; D012261:Ribonucleoproteins / Q000378:metabolism*; D013194:Staining and Labeling; D014746:Vimentin / Q000378:metabolism*
D000911:Antibodies, Monoclonal; C439788:C1 HNRNP; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012261:Ribonucleoproteins; D014746:Vimentin