Biodegradation of the mixtures of 4-chlorophenol and phenol by Comamonas testosteroni CPW301.
A 4-chlorophenol (4-CP)-degrading bacterium, strain CPW301, was isolated from soil and identified as Comamonas testosteroni. This strain dechlorinated and degraded 4-CP via a meta-cleavage pathway. CPW301 could also utilize phenol as a carbon and energy source without the accumulation of any metabolites via the same meta-cleavage pathway. When phenol was added as an additional substrate, CPW301 could degrade 4-CP and phenol simultaneously. The addition of phenol greatly accelerated the degradation of 4-CP due to the increased cell mass. The simultaneous degradation of the 4-CP and phenol is useful not only for enhanced cell growth but also for the bioremediation of both compounds, which are normally present in hazardous waste sites as a mixture.
Bae
H S
HS
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejun, Korea.
Lee
J M
JM
Kim
Y B
YB
Lee
S T
ST
D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D002396:Catechols / Q000378:metabolism; D002733:Chlorophenols / Q000378:metabolism*; D049308:Dioxygenases; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D006256:Hazardous Waste; D010105:Oxygenases / Q000378:metabolism; D019800:Phenol; D010636:Phenols / Q000378:metabolism*; D012988:Soil Microbiology
D002396:Catechols; D002733:Chlorophenols; D006256:Hazardous Waste; D010636:Phenols; D019800:Phenol; C029107:4-chlorophenol; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase