Ultra low temperature cryopreservation of somatic embryogenic cell line of foxtail millet [Setaria italica (L.) Beauv].
Ultra low temperature cryopreservation is one of the methods for preservation of biological material. Until now, a major problem of protoplast culture of Gramineae is the instability of state of the somatic embryogenic cell line. In our experiments, elements affecting the ultra low temperature cryopreservation of somatic embryogenic cell line were studied: components of cryopreserve solution, somatic embryogenic cell line of different subculture time, growth recovery of cryopreserved cell line, and their protoplast cultures. Results demonstrated that the ultra low temperature cryopreservation did not change the properties of protoplast culture, and by using the cryopreserve method, plating efficiency of protoplast culture of cryopreserved cell line was maintained or enhanced.
Dong
J
J
Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai, China.
Xia
Z
Z
D002460:Cell Line; D015925:Cryopreservation; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006822:Hybrid Cells; D006109:Poaceae / Q000196:embryology; D012639:Seeds
D005969:Glutamate Dehydrogenase