Telomerase activity is elevated in early S phase in hamster cells.
Telomerase is a ribonucleoprotein that elongates telomeric repeats de novo. We examined the possibility that telomerase activity is cell cycle regulated by examining telomerase activity in cell cycle synchronized Chinese hamster ovary (CHO) B11 cells. Overall telomerase activity was similar in growing and quiescent cells. Further, cells synchronized in G1, S, or G2/M showed similar levels of telomerase activity. However, a detailed analysis of cells within S phase showed that there was a higher level of telomerase activity in early S phase when compared with other points in the cell cycle. These results suggest a relationship between telomerase activity and cell cycle regulation.
Kruk
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PA
Department of Pathology, University of South Florida, Tampa 33612, USA. pkruk@com1.med.usf.edu
Orren
D K
DK
Bohr
V A
VA
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002453:Cell Cycle / Q000502:physiology; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D016196:S Phase / Q000502:physiology*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D016615:Telomere / Q000235:genetics / Q000378:metabolism
D017931:DNA Primers; D004247:DNA; D019098:Telomerase