title: An NF-kappaB-like transcription factor in axoplasm is rapidly inactivated after nerve injury in Aplysia.
abstract: We found a protein in Aplysia neurons that has many characteristics of the transcription factor NF-kappaB. Thus, the protein recognized a radiolabeled probe containing the kappaB sequence from the human interferon-beta gene enhancer element (PRDII), and the binding was not affected by PRDIV, an ATF-2 enhancer sequence from the same gene. Binding was efficiently inhibited, however, by nonradioactive oligonucleotides containing H2, the kappaB site from the major histocompatibility complex I gene promotor. In addition, recombinant mammalian IkappaB-alpha, which associates specifically with the P65 subunit of NF-kappaB, inhibited the binding to the PRDII probe in a dose-dependent manner. The nuclear form of the Aplysia protein was constitutively active. Axoplasm, however, contained the constitutively active form as well as a latent form. The latter was activated by treatment with deoxycholate under the same conditions as mammalian NF-kappaB. Based on these findings, we believe the protein to be a homolog of NF-kappaB. To investigate the role of apNF-kappaB in the axon, we crushed the peripheral nerves to the body wall. Surprisingly, there was a rapid loss of apNF-kappaB binding at the crush site and, within 15 min, as far as 2.5 cm along the axon. In contrast, exposing either the intact animal or the nervous system in situ to levels of 5-HT that induce synaptic facilitation did not affect apNF-kappaB activity.
authors.affiliation: Departments of Anatomy and Cell Biology, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
authors.forename: M
authors.initials: M
authors.lastname: Povelones
authors.forename: K
authors.initials: K
authors.lastname: Tran
authors.forename: D
authors.initials: D
authors.lastname: Thanos
authors.forename: R T
authors.initials: RT
authors.lastname: Ambron
mesh_terms: D000818:Animals; D001048:Aplysia / Q000502:physiology*; D001369:Axons / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology; D013997:Time Factors; D020196:Trauma, Nervous System
chemical_list: D002352:Carrier Proteins; D016328:NF-kappa B; D012701:Serotonin