title: Generation of small insert genomic FISH probes with high signal intensity suitable for deletion mapping.
abstract: We have developed a method to generate FISH probes from small (2-4 kb) nonrepetitive genomic restriction fragments. The probes showed a high hybridization efficiency of up to 90% in metaphase cells from normal peripheral blood. With the use of these probes, homozygous as well as hemizygous 9p deletions were reliably identified in nine leukemia-derived cell lines.
authors.affiliation: Abteilung für Hämatologie und Onkologie, Georg-August-Universität Götingen, Germany. mdreylin@mdv.gwdg.de
authors.forename: M H
authors.initials: MH
authors.lastname: Dreyling
authors.forename: O I
authors.initials: OI
authors.lastname: Olopade
authors.forename: S K
authors.initials: SK
authors.lastname: Bohlander
mesh_terms: D002460:Cell Line; D002872:Chromosome Deletion; D002899:Chromosomes, Human, Pair 9; D015342:DNA Probes; D018922:HL-60 Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016254:Mutagenesis, Insertional
chemical_list: D015342:DNA Probes