Postadhesive technique for archival paraffin sections.
We present a postadhesive protocol for adhering paraffin sections of archival material to microscope slides. Appropriately posttreated sections, subsequently processed for immunohistochemistry, remained attached to the slides and were well preserved with no signs of artifacts, such as scratching and shrinkage. The immunohistochemical staining was intense and antigen-specific without nonspecific background. Specific staining intensity was equal to that produced in untreated control sections; however, the latter became partially or fully detached from the slides. The postadhesion protocol may be used with modern techniques and is recommended for reclaiming use of otherwise unsuitable paraffin sections of archival material.
Pateraki
M
M
Department of Pathology, General Hospital of Athens, Greece.
Kontogeorgos
G
G
D000941:Antigens / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008867:Microtomy; D016612:Paraffin Embedding
D000941:Antigens