title: Structural analysis of a bovine arginine tRNA(CCG) gene.
abstract: A bovine genomic clone containing a 17.4-kb DNA fragment was isolated and found to contain a solitary arginine tRNA gene with an anticodon of CCG that has a 100% identity to its cognate tRNA. This arginine tRNA gene, symbolized as TRR4, has a characteristic internal split promoter and a typical termination site for RNA polymerase III. The tRNA gene was transcribed in vitro by RNA polymerase III using a HeLa cell-free extract to yield a mature-sized tRNA product. The gene was mapped to bovine chromosome 19 using a panel of bovine-rodent somatic cell hybrid DNAs.
authors.affiliation: Department of Biological Sciences, University of North Texas, Denton 76203, USA.
authors.forename: A C
authors.initials: AC
authors.lastname: Eubanks
authors.forename: M J
authors.initials: MJ
authors.lastname: Roeder
authors.forename: I L
authors.initials: IL
authors.lastname: Pirtle
authors.forename: R M
authors.initials: RM
authors.lastname: Pirtle
mesh_terms: D000818:Animals; D000926:Anticodon; D001120:Arginine / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D012343:RNA, Transfer / Q000235:genetics*
chemical_list: D000926:Anticodon; D004247:DNA; D012343:RNA, Transfer; D001120:Arginine