Toxicological effects of beryllium on platelets and vascular endothelium.
Although ample research has described the toxic effects of the metal beryllium on the respiratory apparatus, less is known about its effects on the vascular apparatus, including pulmonary blood vessels. We investigated the in vitro effects of beryllium on endothelial vascular adenosine diphosphatase activity and prostacyclin production in bovine aortic endothelium, and on nitric oxide release in isolated rabbit arteries. Rabbit and human platelet responsiveness was also evaluated. Beryllium inhibited vascular endothelial adenosine diphosphatase activity, prostacyclin production, and nitric oxide release, thus inducing functional alterations in vascular endothelial cells. It also induced platelet hyperreactivity to arachidonic acid, as shown by a lowering of the threshold of aggregating concentration and by concurrently increasing thromboxane production. In contrast, beryllium left the response to aggregating and nonaggregating concentrations of ADP and collagen unchanged. These findings show that beryllium may impair some vascular endothelial functions and alter the interaction between platelet and endothelial mediators.
Togna
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Institute of Pharmacology, University of Rome La Sapienza, Italy.
Togna
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Russo
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Caprino
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D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects; D001081:Apyrase / Q000378:metabolism; D016718:Arachidonic Acid / Q000494:pharmacology; D001608:Beryllium / Q000633:toxicity*; D001792:Blood Platelets / Q000187:drug effects*; D002417:Cattle; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D009569:Nitric Oxide / Q000096:biosynthesis; D011817:Rabbits; D013931:Thromboxanes / Q000096:biosynthesis
D013931:Thromboxanes; D016718:Arachidonic Acid; D009569:Nitric Oxide; D011464:Epoprostenol; D001081:Apyrase; D001608:Beryllium