title: A circadian enhancer mediates PER-dependent mRNA cycling in Drosophila melanogaster.
abstract: Genes expressed under circadian-clock control are found in organisms ranging from prokaryotes to humans. In Drosophila melanogaster, the period (per) gene, which is required for clock function, is transcribed in a circadian manner. We have identified a circadian transcriptional enhancer within a 69-bp DNA fragment upstream of the per gene. This enhancer drives high-amplitude mRNA cycling under light-dark-cycling or constant-dark conditions, and this activity is per protein (PER) dependent. An E-box sequence within this 69-bp fragment is necessary for high-level expression, but not for rhythmic expression, indicating that PER mediates circadian transcription through other sequences in this fragment.
authors.affiliation: Department of Biology, Texas A & M University, College Station 77843-3258, USA.
authors.forename: H
authors.initials: H
authors.lastname: Hao
authors.forename: D L
authors.initials: DL
authors.lastname: Allen
authors.forename: P E
authors.initials: PE
authors.lastname: Hardin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002940:Circadian Rhythm; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000502:physiology; D005246:Feedback; D005786:Gene Expression Regulation; D017344:Genes, Insect; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D056950:Period Circadian Proteins; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D012333:RNA, Messenger