[Glycohistochemical analysis of rat eyelid epithelium].
The glycoconjugates in eyelids of adult rats were examined by lectin histochemistry and in situ hybridization histochemistry. Since Maackia amurensis lectin II and jacalin bound to the cell membranes of basal and apical epithelial cells, sialic acid alpha 2,3 galactose (Gal) beta 1,3 N-acetylgalactosamine (GalNAc) sequence is present in the glycoconjugates of their cell membranes. Peanut agglutinin bound to the cell membranes of spinous cells in the middle of the epithelium, suggesting that Gal beta 1, 3 GalNAc sequence is present in their glycoconjugates. The mRNA of Gal beta 1,3 GalNAc alpha 2,3-sialyltransferase was detected in the cytoplasm of the epithelial cells other than the basal cells. This observation suggests that sialoglycoconjugates may be newly synthesized in the spinous and apical cells, while the glycoconjugates in the cell membranes of basal cells may be produced at an early stage of development and are stable without turnover.
Uehara
F
F
Department of Ophthalmology, Kagoshima University Faculty of Medicine, Japan.
Yanagita
T
T
Iwakiri
N
N
Ohba
N
N
D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D005143:Eyelids / Q000737:chemistry* / Q000166:cytology; D006001:Glycoconjugates / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods; D037102:Lectins / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012799:Sialyltransferases / Q000235:genetics
D006001:Glycoconjugates; D037102:Lectins; D012333:RNA, Messenger; D012799:Sialyltransferases