title: RAB22 and RAB163/mouse BRCA2: proteins that specifically interact with the RAD51 protein.
abstract: The human RAD51 protein is a homologue of the bacteria RecA and yeast RAD51 proteins that are involved in homologous recombination and DNA repair. RAD51 interacts with proteins involved in recombination and also with tumor suppressor proteins p53 and breast cancer susceptibility gene 1 (BRCA1). We have used the yeast two-hybrid system to clone murine cDNA sequences that encode two RAD51-associated molecules, RAB22 and RAB163. RAB163 encodes the C-terminal portion of mouse BRCA2, the homologue of the second breast cancer susceptibility gene protein in humans, demonstrating an in vitro association between RAD51 and BRCA2. RAB22 is a novel gene product that also interacts with RAD51 in vitro. To detect RAD51 interactions in vivo, we developed a transient nuclear focus assay that was used to demonstrate a complete colocalization of RAB22 with RAD51 in large nuclear foci.
authors.affiliation: Howard Hughes Medical Institute, Children's Hospital, Boston, MA 02115, USA.
authors.forename: R
authors.initials: R
authors.lastname: Mizuta
authors.forename: J M
authors.initials: JM
authors.lastname: LaSalle
authors.forename: H L
authors.initials: HL
authors.lastname: Cheng
authors.forename: A
authors.initials: A
authors.lastname: Shinohara
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: N
authors.initials: N
authors.lastname: Copeland
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: M
authors.initials: M
authors.lastname: Lalande
authors.forename: F W
authors.initials: FW
authors.lastname: Alt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D024682:BRCA2 Protein; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004260:DNA Repair; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D051135:Rad51 Recombinase; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D024682:BRCA2 Protein; C106711:BRCA2 protein, mouse; D002352:Carrier Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C106710:Rad51ap1 protein, mouse; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C495151:RAD51 protein, S cerevisiae; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; C495154:Rad51 protein, mouse