Effect of lipid emulsion on IL-2 production by mononuclear cells of newborn infants and adults.
The in vitro effect of a lipid emulsion (intralipid) on interleukin-2 (IL-2) production by cord blood mononuclear cells (CBMC) of preterm and term newborn infants was examined and compared to that of peripheral blood mononuclear cells (PBMC) of adults. Intralipid, added at concentrations accepted in clinical practice, caused a dose-dependent inhibition of IL-2 activity tested by bioassay. IL-2 levels, tested by radioimmunoassay (RIA), were found to be reduced only in supernatants derived from CBMC of term infants and not in those derived from MC of preterm infants or adults. The capacity of the IL-2 dependent cell line CTLL-2 to respond to IL-2 was abolished in the presence of intralipid, suggesting an interference with the binding of IL-2 to its receptor on these cells. It is conceivable that administration of intralipid to preterm infants may interfere with the binding of IL-2 to the specific receptors on their activated lymphocytes, with a possible subsequent suppression of their immune response.
Sirota
L
L
Neonatology Unit, Sackler School of Medicine, Tel Aviv University, Israel.
Straussberg
R
R
Notti
I
I
Bessler
H
H
D000328:Adult; D001681:Biological Assay; D002460:Cell Line / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005217:Fat Emulsions, Intravenous / Q000009:adverse effects*; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn / Q000276:immunology* / Q000378:metabolism; D007234:Infant, Premature / Q000276:immunology; D007376:Interleukin-2 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D010288:Parenteral Nutrition / Q000009:adverse effects*
D005217:Fat Emulsions, Intravenous; D007376:Interleukin-2