Endothelial cell monolayer dysfunction caused by oxidized low density lipoprotein: attenuation by oleic acid.
Oleic acid (18:1) may exert beneficial effects on the pathogenesis of vascular disease by a variety of mechanisms. To determine if 18:1 exerts direct protective effects on vascular endothelial cells, porcine pulmonary artery endothelial cells (PAEC) were supplemented with 0.1 mM 18:1, gamma-linolenic acid (18:3), or ethanol vehicle (ETOH) prior to treatment with low density lipoprotein (LDL), or CU(2+)-oxidized LDL (OXLDL). Treatment with neither LDL nor OXLDL (100 micrograms protein/ml) for 24-48 h caused PAEC cytotoxicity, whereas OXLDL, but not LDL, caused derangements in PAEC actin microfilament architecture and monolayer barrier dysfunction. Supplementation with 18:1, but not 18:3, attenuated derangements caused by OXLDL and lysophosphatidylcholine, a component of OXLDL. These results demonstrate that monounsaturated fatty acids directly alter the response of vascular endothelial cells to OXLDL and may retard the atherosclerotic process by decreasing the efflux of macromolecules (e.g. LDL) into the vessel wall.
Karman
R J
RJ
Department of Medicine, Indiana University, Indianapolis, USA.
Garcia
J G
JG
Hart
C M
CM
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology; D002478:Cells, Cultured; D004347:Drug Interactions; D004730:Endothelium, Vascular / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D019301:Oleic Acid / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010539:Permeability / Q000187:drug effects; D011651:Pulmonary Artery / Q000166:cytology; D013552:Swine
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D019301:Oleic Acid