title: Action of trypsin:chymotrypsin (Chymoral forte DS) preparation on acute-phase proteins following burn injury in humans.
abstract: A study was carried out to investigate the efficacy of trypsin: chymotrypsin (Chymoral forte DS) preparation on burn patients by analysing the changes taking place in serum acute-phase proteins. Serum proteins were analysed qualitatively and quantitatively for both control and enzyme-treated groups by the methods of Western Blot, ELISA and Turbidimetric assays. Furthermore, the trypsin inhibitory capacity (TIC) of the sera was also determined. Significant differences were observed between a control group of patients and a parallel group treated with trypsin: chymotrypsin preparation. During the first phase of burn wounds an initial rise was seen in C-reactive protein, alpha 1-antitrypsin, alpha 2-macroglobulin and TIC in both the groups. In the following days, enzyme preparation inhibited the rise in C-reactive protein titres and enhanced the rise in alpha 1-antitrypsin, alpha 2-macroglobulin and TIC. The above studies clearly indicate that the changes in serum acute-phase proteins between the control and treated groups reflect the anti-inflammatory activity and hence the therapeutic efficacy of Chymoral forte DS.
authors.affiliation: Department of Biotechnology, Central Leather Research Institute, Adyar, India.
authors.forename: B
authors.initials: B
authors.lastname: Latha
authors.forename: K M
authors.initials: KM
authors.lastname: Ramakrishnan
authors.forename: V
authors.initials: V
authors.lastname: Jayaraman
authors.forename: M
authors.initials: M
authors.lastname: Babu
mesh_terms: D000209:Acute-Phase Proteins / Q000187:drug effects* / Q000378:metabolism; D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001797:Blood Protein Electrophoresis; D015153:Blotting, Western; D002056:Burns / Q000097:blood / Q000188:drug therapy*; D002918:Chymotrypsin / Q000627:therapeutic use*; D004338:Drug Combinations; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008875:Middle Aged; D014357:Trypsin / Q000627:therapeutic use*
chemical_list: D000209:Acute-Phase Proteins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004338:Drug Combinations; C007059:chymotrypsin, trypsin drug combination; D002918:Chymotrypsin; D014357:Trypsin