Changes in 31P-relaxation times during organ preservation: observations on cold stored human donor livers.
During cold preservation for transplantation the tissue hydration state changes. It is not known whether such changes lead to altered relaxation times of 31P nuclei with potential consequences for the quantification of tissue metabolites. Therefore, 31P spectroscopic and proton T1 relaxometric measurements were performed on 42 isolated human donor livers shortly before implantation. The results demonstrate that 31P T1 relaxation times change during preservation for clinical transplantation, thus quantification of tissue metabolites in cold stored donor livers may be in part dependent on the tissue hydration state. Furthermore, it appeared that changes in tissue hydration state especially affect the physico-chemical characteristics of the intracellular fluid compartment. This study indicates that reliable spectroscopic quantification of tissue metabolites, particularly during sequential spectroscopic measurements in cold stored donor organs is best warranted under fully relaxed conditions.
Wolf
R F
RF
Department of Surgery, University Hospital, Groningen, The Netherlands.
Slooff
M J
MJ
Go
K G
KG
Kamman
R L
RL
D000241:Adenosine / Q000008:administration & dosage; D000493:Allopurinol / Q000008:administration & dosage; D001011:Aorta; D019540:Area Under Curve; D001659:Biliary Tract; D001834:Body Water / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D015925:Cryopreservation; D005583:Fourier Analysis; D005978:Glutathione / Q000008:administration & dosage; D006499:Hepatic Artery; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007328:Insulin / Q000008:administration & dosage; D007424:Intracellular Fluid / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D016031:Liver Transplantation / Q000502:physiology; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010758:Phosphorus / Q000378:metabolism*; D010759:Phosphorus Isotopes; D011169:Portal Vein; D011887:Raffinose / Q000008:administration & dosage; D015203:Reproducibility of Results; D013057:Spectrum Analysis
D007328:Insulin; D019188:Organ Preservation Solutions; D010759:Phosphorus Isotopes; C055206:University of Wisconsin-lactobionate solution; D010758:Phosphorus; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose