Isolation and characterization of cDNA clones with enhanced expression in tobacco plants expressing the rice homeobox gene, OSH1.
We have used subtractive hybridization to isolate cDNA clones whose expression were up-regulated in transgenic tobacco ectopically expressing the rice homeobox gene, OSH1. Thirty-nine distinct cDNA clones, which we term HRGs (Homeobox Regulated Genes), were identified. Some of them were specifically expressed in transformants, indicating that their expression was possibly regulated by transgene.
Tamaoki
M
M
Nagoya University, BioScience Center, Aichi, Japan.
Yamamoto
N
N
Matsuoka
M
M
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005801:Genes, Homeobox; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics; D015534:Trans-Activators; D015854:Up-Regulation
D018076:DNA, Complementary; D018398:Homeodomain Proteins; C099194:OSH1 protein, Oryza sativa; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D015534:Trans-Activators