title: Molecular analysis of katG gene mutations in strains of Mycobacterium tuberculosis complex from Africa.
abstract: A sample of 124 isoniazid (INH)-resistant and 88 susceptible strains of Mycobacterium tuberculosis complex from south, central, and west Africa was analyzed by direct sequence analysis and PCR-restriction fragment length polymorphism analysis of their catalase-peroxidase (katG) genes. Point mutations at codon 315 were found in the genomes of 64% of INH-resistant strains, but no complete deletions were identified. Mutations at codon 463 were independent of INH resistance and were linked to the geographic origins of the strains.
authors.affiliation: Department of General Pediatrics, Children's Hospital, Heidelberg University, Germany. ic1@aixterm1.urz.uni-heidelberg.de
authors.forename: W H
authors.initials: WH
authors.lastname: Haas
authors.forename: K
authors.initials: K
authors.lastname: Schilke
authors.forename: J
authors.initials: J
authors.lastname: Brand
authors.forename: B
authors.initials: B
authors.lastname: Amthor
authors.forename: K
authors.initials: K
authors.lastname: Weyer
authors.forename: P B
authors.initials: PB
authors.lastname: Fourie
authors.forename: G
authors.initials: G
authors.lastname: Bretzel
authors.forename: V
authors.initials: V
authors.lastname: Sticht-Groh
authors.forename: H J
authors.initials: HJ
authors.lastname: Bremer
mesh_terms: D000349:Africa; D002374:Catalase / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D009195:Peroxidase / Q000235:genetics*; D017354:Point Mutation
chemical_list: D002374:Catalase; D009195:Peroxidase