title: Surface-related triggering of the neutrophil respiratory burst. Characterization of the response induced by IgG adsorbed to hydrophilic and hydrophobic glass surfaces.
abstract: Hydrophilic and hydrophobic glass surfaces precoated with human albumin, fibrinogen, or IgG were investigated with respect to their ability to activate the neutrophil NADPH-oxidase. We found that IgG-coated surfaces induced a substantial and prolonged neutrophil production of reactive oxygen species (ROS). When a hydrophilic surface was used to support protein binding, a somewhat lower neutrophil response (around 35%) was obtained, compared with the response induced by IgG on a hydrophobic surface. The production of ROS was completely eliminated when cytochalasin B was added to the measuring system, suggesting the involvement of the cell cytoskeleton in the activation process. The relation between the intra- and extracellular generation of ROS was further assessed, and we found that most of the ROS produced were released from the cells, in agreement with a model in which the activating surfaces induce a 'frustrated' phagocytic response. Serum totally inhibited 'frustrated' phagocytosis provided that the IgG molecules were sticking to a hydrophilic surface.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Elwing
authors.forename: A
authors.initials: A
authors.lastname: Karlsson
authors.forename: G
authors.initials: G
authors.lastname: Nimeri
authors.forename: C
authors.initials: C
authors.lastname: Dahlgren
mesh_terms: D000418:Albumins / Q000276:immunology; D001769:Blood / Q000276:immunology; D003571:Cytochalasin B / Q000494:pharmacology; D005340:Fibrinogen / Q000276:immunology; D005434:Flow Cytometry; D005898:Glass; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008163:Luminescent Measurements; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism*; D010587:Phagocytosis / Q000276:immunology; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000276:immunology; D013499:Surface Properties; D014867:Water / Q000378:metabolism*
chemical_list: D000418:Albumins; D007074:Immunoglobulin G; D017382:Reactive Oxygen Species; D014867:Water; D003571:Cytochalasin B; D005340:Fibrinogen; D019255:NADPH Oxidases