title: Inhibitory effect of 0 degree C storage on the proliferation of Yersinia enterocolitica in donated blood.
abstract: BACKGROUND
  Yersinia enterocolitica is frequently identified in cases of bacterial sepsis due to red cell transfusion. One of the features that makes Y. enterocolitica particularly dangerous is that, unlike most other bacterial contaminants of blood components, this organism can actively multiply in currently recommended refrigerator temperatures (1-6 degrees C). The effect of a colder than normal storage temperature on Y. enterocolitica growth was investigated to determine whether bacteria growth could be reduced or inhibited at 0 degree C.
  
  
  METHODS
  Twenty-four units of freshly collected donated blood were obtained. Three sets of 7 units each were inoculated with Y. enterocolitica O:3, Y. enterocolitica O:20, and Y. enterocolitica O:5, 27, respectively. The remaining 3 units served as uninoculated controls. Each of the 24 bags was split into two equal aliquots, with one aliquot stored at 4 degrees C and the other at 0 degree C. Bacteria growth was measured twice weekly for 6 weeks. Endotoxin and hemoglobin levels were also measured at selected intervals.
  
  
  RESULTS
  Bacteria growth was detected earlier and in higher concentrations in the aliquots stored at 4 degrees C. Twenty-two of the 42 inoculated aliquots had measureable bacteria growth. Thirteen aliquots had been maintained at 4 degrees C, and nine had been stored at 0 degree C. Sixteen of these 22 aliquots were matched pairs. Exponential growth was detected after 14 to 32 days in the 4 degrees C aliquots and after 28 to 39 days in the 0 degree C aliquots. Final bacteria counts were much higher in the 4 degrees C aliquots (10(5)-10(14) colony-forming units/mL) than in the 0 degree C aliquots (10(1)-10(4) colony-forming units/mL) on Day 42. Endotoxin was present in all 13 of the 4 degrees C aliquots with actively growing Y. enterocolitica.
  
  
  CONCLUSIONS
  Storage of red cells at 0 degree C markedly prolongs the time required for Y. enterocolitica to achieve exponential grwoth and results in lower concentrations of bacteria.
authors.affiliation: Department of Pathology, University of Texas Southwestern Medical Center, Dallas, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Bradley
authors.forename: R M
authors.initials: RM
authors.lastname: Gander
authors.forename: S K
authors.initials: SK
authors.lastname: Patel
authors.forename: H S
authors.initials: HS
authors.lastname: Kaplan
mesh_terms: D001769:Blood / Q000382:microbiology; D001782:Blood Donors; D001793:Blood Preservation; D015925:Cryopreservation; D004731:Endotoxins / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D013997:Time Factors; D015008:Yersinia enterocolitica / Q000254:growth & development* / Q000378:metabolism
chemical_list: D004731:Endotoxins; D006454:Hemoglobins