title: Molecular cloning and sequencing of the cDNA encoding the catalytic subunit of mouse glutamate-cysteine ligase.
abstract: Reverse transcription-polymerase chain reaction (RT-PCR) was used for the enzymatic synthesis of cDNA sequences encompassing the open reading frame for the catalytic subunit of mouse kidney glutamate-cysteine ligase (Glclc). Comparison of the mouse Glclc cDNA sequence and predicted protein sequence with that of rat Glclc and human GLCLC revealed between 94.8% and 88.4% cDNA homology and 98.4% to 95% amino acid identity, respectively.
authors.affiliation: Department of Environmental Health, University of Washington, Seattle, USA.
authors.forename: L L
authors.initials: LL
authors.lastname: Reid
authors.forename: D
authors.initials: D
authors.lastname: Botta
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: E P
authors.initials: EP
authors.lastname: Gallagher
authors.forename: T J
authors.initials: TJ
authors.lastname: Kavanagh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D005721:Glutamate-Cysteine Ligase / Q000235:genetics*; D007668:Kidney / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D005721:Glutamate-Cysteine Ligase