title: Sequence variation in the early genes E1E4, E6 and E7 of human papilloma virus type 6.
abstract: The majority of condylomata acuminata (anogenital warts) are caused by infection with Human Papilloma Virus type 6 (HPV-6). We have sequenced the HPV-6 early genes, E1-E4, E6 and E7 from wart biopsy DNA samples sourced from the UK and USA and derived a consensus sequence for these genes and the proteins they encode. When compared to the prototype HPV-6b sequence, published over 12 years ago, the E1-E4 consensus sequence showed 3/91 (3.3%) amino acid changes, the E6 consensus sequence showed 1/150 (0.7%) changes and the E7 consensus sequence showed 1/98 (1.0%) changes. Since many of the early gene sequences from biopsy material were more similar to the HPV-6a subtype than HPV-6b, this data supports the use of HPV-6a as the HPV-6 prototype.
authors.affiliation: British Biotech Pharmaceuticals Ltd., Oxford, UK.
authors.forename: D
authors.initials: D
authors.lastname: Krige
authors.forename: H R
authors.initials: HR
authors.lastname: Mills
authors.forename: E L
authors.initials: EL
authors.lastname: Berrie
authors.forename: N C
authors.initials: NC
authors.lastname: Doherty
authors.forename: D K
authors.initials: DK
authors.lastname: Jones
authors.forename: C A
authors.initials: CA
authors.lastname: Ryan
authors.forename: H
authors.initials: H
authors.lastname: Davies
authors.forename: S
authors.initials: S
authors.lastname: Myint
authors.forename: D J
authors.initials: DJ
authors.lastname: McCance
authors.forename: G T
authors.initials: GT
authors.lastname: Layton
authors.forename: T J
authors.initials: TJ
authors.lastname: French
mesh_terms: D003218:Condylomata Acuminata / Q000473:pathology / Q000821:virology; D016384:Consensus Sequence; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D005814:Genes, Viral; D006801:Humans; D027383:Papillomaviridae / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins