Efficient preparation of single-stranded DNA for in vitro selection.
In vitro selection of aptamers requires the reliable enzymatic preparation of large amounts of (+) single-stranded DNA molecules. This can be achieved by selective enzymatic digest of 5'-phosphorylated (-) strands from PCR products, a method already widely used in sequencing of PCR products. Here we present an adaptation of this method to prepare large pools of single-stranded DNA molecules for in vitro selection.
Kujau
M J
MJ
Hans-Knöll-Institut für Naturstoff-Forschung, Abt.Zell- und Molekularbiologie, Jena, Germany.
Wölfl
S
S
D004277:DNA, Single-Stranded / Q000737:chemistry / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
D004277:DNA, Single-Stranded