title: S-100-positive glial cells are involved in the regeneration of the visual pathway of teleosts.
abstract: Glial cells in the normal and regenerating visual pathways of Tinca tinca (Cyprinid, Teleost) were studied by labelling with anti-S-100 antibody. In normal fish, S-100-positive bipolar cells were found in the optic nerve, optic tract, and in the diencephalic visual pathways. After crushing the left optic nerve, the distribution and the number of S-100-immunoreactive cells were modified. In the injured nerve, 7 to 15 days after crushing no immunoreactive cell bodies were found in the crushed area, but a greater number of S-100-positive cells were found on both sides of the injured area. Sixty days after crushing, positive cells penetrating the crushed area were observed; the normal pattern was almost restored 200 days after crushing. In the diencephalon, 25 days after crushing, the number of S-100-positive cells increased remarkably and the most intense immunostaining of glial processes was observed 60 days after crushing. The density of S-100-labelled cells decreased after 4 months postcrushing. However, in the optic tectum no changes were observed. The increase of glial cells in the lesioned visual pathway suggests that they could play an important role in axonal regeneration after crushing.
authors.affiliation: Departamento de Biología Celular y Patología, Universidad de Salamanca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Velasco
authors.forename: J G
authors.initials: JG
authors.lastname: Briñón
authors.forename: E
authors.initials: E
authors.lastname: Caminos
authors.forename: J M
authors.initials: JM
authors.lastname: Lara
authors.forename: J
authors.initials: J
authors.lastname: Aijón
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D009416:Nerve Regeneration / Q000502:physiology*; D009457:Neuroglia / Q000502:physiology*; D009900:Optic Nerve / Q000378:metabolism*; D009418:S100 Proteins / Q000378:metabolism*; D014795:Visual Pathways / Q000502:physiology*
chemical_list: D009418:S100 Proteins