title: Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats.
abstract: The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Isolated granulosa cells were cultured in serum-free medium, different stimuli added for periods of 48 h, and 3H-thymidine incorporation was measured. Both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) inhibited 3H-thymidine incorporation by cultured granulosa cells in a dose-dependent manner (FSH: 10, 100, 200 ng/mL = 26, 41, 49% inhibition, respectively; LH: 0.1, 1, 10 ng/mL = 11, 37, 75% inhibition, respectively). On the other hand, estradiol was found to stimulate 3H-thymidine incorporation in granulosa cells (Estradiol: 5, 50, 500 ng/mL = 17, 37, 76% stimulation, respectively). In luteal cells, the rate of basal 3H-thymidine incorporation was very low (granulosa cells: 2560 +/- 310; luteal cells: 661 +/- 92 cpm/100,000 cells) and not modified by any stimulus. To determine the possible production of an inhibitory growth factor by the early corpus luteum, 3H-thymidine incorporation by granulosa cells was assessed in the presence of 10% conditioned media (CM) recovered from luteal cell cultures. A marked inhibition both in basal and estradiol-stimulated 3H-thymidine incorporation was observed (74 and 76% of inhibition, respectively). Results suggest that an inhibitory growth factor produced by luteal cells after luteinizing gonadotrophin stimulus could be involved in the differentiation of growing follicles to corpus luteum.
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME)-CONICET, Buenos Aires, Argentina.
authors.forename: C V
authors.initials: CV
authors.lastname: Andreu
authors.forename: C A
authors.initials: CA
authors.lastname: Buscaglia
authors.forename: F
authors.initials: F
authors.lastname: Parborell
authors.forename: P
authors.initials: P
authors.lastname: Stein
authors.forename: M
authors.initials: M
authors.lastname: Tesone
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism; D017077:Culture Media, Conditioned; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D006064:Gonadotropins, Equine; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology* / Q000378:metabolism; D010053:Ovary / Q000166:cytology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013256:Steroids / Q000096:biosynthesis; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D006062:Gonadotropins; D006064:Gonadotropins, Equine; D013256:Steroids; D004247:DNA; D013936:Thymidine