title: Hepatic glutaminase gene expression in the tumor-bearing rat.
abstract: Previous studies have documented an increase in hepatic plasma membrane glutamine transport in the tumor-bearing rat, but the effects of tumor burden on hepatic glutaminase expression have not been carefully studied. The purpose of this study was to examine the effects of tumor burden and food intake on hepatic glutaminase expression. Rats were implanted with syngeneic methylcholanthrene-induced fibrosarcoma tumor tissue; control rats were sham operated and pair-fed every 24 hr. Northern blotting was used to assay the effect of tumor burden and fasting on hepatic glutaminase mRNA levels, using beta-actin mRNA as a control. Hepatic glutaminase mRNA levels in livers of pair-fed controls were found to be 4-fold greater than levels in livers of tumor-bearing animals. Examination of food intake patterns in these animals indicated that pair-fed controls ate their allotted chow quickly while tumor-bearing rats ate small amounts throughout each 24 hr period. This observation suggested that the differences in glutaminase mRNA levels may be due to a period of fasting by pair-fed animals which was not experienced by the tumor-bearing group. Hepatic glutaminase mRNA levels rapidly increased in normal rats during acute fasting to levels 5.5-fold greater than fed animals. Glucose feeding and insulin injection rapidly reversed the effect of fasting on hepatic glutaminase mRNA levels in normal rats. Tumor-bearing rats also exhibited upregulation of hepatic glutaminase mRNA levels in response to fasting.
  
  
  CONCLUSIONS
  (1) Tumor burden itself does not alter hepatic glutaminase expression, at least at the pre-translational level. Instead, differences in hepatic glutaminase mRNA content are due to differences in food intake patterns. (2) Hepatic glutaminase mRNA levels are rapidly upregulated in response to fasting, an effect which appears to be linked to a decrease in plasma insulin concentrations. Because tumor-bearing rats eat regularly over a 24 hr period (albeit in small increments), thereby maintaining the plasma insulin concentration, hepatic glutaminase mRNA may not rise as it does in pair-fed controls whose daily chow intake is complete within hours of food allocation. (3) This study indicates that differences in the timing of food intake between tumor-bearing rats and pair-fed controls can alter the expression of genes that are influenced by nutrient availability. These differences should be taken into account when designing studies which involve pair-feeding to control nutrient intake.
authors.affiliation: Massachusetts General Hospital, Boston 02114, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Elgadi
authors.forename: W W
authors.initials: WW
authors.lastname: Souba
authors.forename: B P
authors.initials: BP
authors.lastname: Bode
authors.forename: S F
authors.initials: SF
authors.lastname: Abcouwer
mesh_terms: D000208:Acute Disease; D000818:Animals; D005354:Fibrosarcoma / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics*; D015870:Gene Expression; D005947:Glucose / Q000494:pharmacology; D005972:Glutaminase / Q000235:genetics*; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008748:Methylcholanthrene; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D013217:Starvation / Q000201:enzymology
chemical_list: D007328:Insulin; D008748:Methylcholanthrene; D005972:Glutaminase; D005947:Glucose