title: S-phase function of Drosophila cyclin A and its downregulation in G1 phase.
abstract: BACKGROUND
  Cyclin E is the normal inducer of S phase in G1 cells of Drosophila embryos. Stable G1 quiescence requires the downregulation both of cyclin E and of other factors that can bypass the normal regulation of cell cycle progression.
  
  
  RESULTS
  High-level expression of cyclin A triggered the G1/S transition in wild-type embryos and in mutant embryos lacking cyclin E. Three types of control downregulated this activity of cyclin A. First, cyclin destruction limited the accumulation of cyclin A protein in G1. Second, inhibitory phosphorylation of cdc2, the kinase partner of cyclin A, reduced the S-phase promoting activity of cyclin A in G1. Third, rux, a protein with unknown biochemical function, limited cyclin A function in G1. Overexpression of rux blocked S phase induction by coexpressed cyclin A and promoted the degradation of cyclin A. Rux also prevented a stable cyclin A mutant from inducing S phase, indicating that inhibition does not require cyclin destruction, and drove the nuclear localization of cyclin A.
  
  
  CONCLUSIONS
  Cyclin A can drive the G1/S transition, but this function is suppressed by three types of control: cyclin A destruction, inhibitory phosphorylation of cdc2, and inhibition by rux. The partly redundant contributions of these three inhibitory mechanisms safeguard the stability of G1 quiescence until the induction of cyclin E. The action of rux during G1 resembles the action of inhibitors of mitotic kinases present during G1 in yeast, although no obvious sequence similarity exists.
authors.affiliation: Department of Genetics, University of Cologne, Weyertal 121, 50931, Cologne, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Sprenger
authors.forename: N
authors.initials: N
authors.lastname: Yakubovich
authors.forename: P H
authors.initials: PH
authors.lastname: O'Farrell
mesh_terms: D000818:Animals; D016213:Cyclins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015536:Down-Regulation; D004330:Drosophila / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins; D005136:Eye Proteins / Q000235:genetics; D016193:G1 Phase / Q000502:physiology*; D016196:S Phase / Q000502:physiology*
chemical_list: D016213:Cyclins; D029721:Drosophila Proteins; D005136:Eye Proteins; C088084:rux protein, Drosophila