v-Rel activates the proto-oncogene c-Jun promoter: a correlation with its transforming activity.
v-Rel is a transforming protein of the reticuloendotheliosis virus, and is a transcription factor regulating various cellular genes. We found that v-Rel activates the promoter of the proto-oncogene c-jun in a transient transfection assay system. Moreover, the expression of endogenous c-jun was augmented in cells expressing exogenous v-Rel, but not c-Rel. The transcriptional activities of v-Rel to the tested promoters containing the kB-site are lower than that of c-Rel, but that to the c-jun promoter was much higher than that of c-Rel. The N-terminal DNA binding domain of v-Rel, which is responsible for its high transforming activity of v-Rel was also responsible for the high transcriptional activity to the c-jun promoter. Thus, the activity of v-Rel upon the c-jun promoter correlates well with its transforming ability. Since c-Jun plays pivotal roles on cell proliferation in various types of cells, the activation of c-jun expression by v-Rel may be an essential step for the oncogenic transformation caused by v-Rel.
Fujii
M
M
Department of Immunotherapeutics, Tokyo Medical and Dental University, Japan.
Minamino
T
T
Nomura
M
M
Miyamoto
K
K
Tanaka
J
J
Seiki
M
M
D001665:Binding Sites; D015500:Chloramphenicol O-Acetyltransferase; D015972:Gene Expression Regulation, Neoplastic; D016758:Genes, jun; D006367:HeLa Cells; D006801:Humans; D016297:Mutagenesis, Site-Directed; D020572:Oncogene Proteins v-rel; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000096:biosynthesis; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
C000715470:MAS1 protein, human; D020572:Oncogene Proteins v-rel; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun; D011993:Recombinant Fusion Proteins; D015682:Retroviridae Proteins, Oncogenic; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D015500:Chloramphenicol O-Acetyltransferase