title: The use of a multitracer technique for the studies of the uptake and retention of trace elements in rats.
abstract: The uptake by, and distribution and retention of radioactive isotopes in various organs of Wistar rats were examined using the multitracer technique. A hydrocholoric acid solution (pH 3) of a carrier-free radioactive multitracer was prepared from gold foil irradiated with a 14N beam of 135 MeV nucleon. The solution was administered orally to 12 7-week-old male rats. Urine and faeces were collected and each group of three rats was killed at 1, 2, 3 or 6 days after administration. The percentage of administered dose of the 17 elements, Mn, Co, Zn, As, Rb, Sr, Y, Eu, Gd, Er, Tm, Yb, Lu, W, Re, Ir and Pt in the organs blood and excreta were determined using gamma-ray spectrometry. Each element revealed its characteristic distribution among the different organs, including the blood. These results are discussed and compared with those of single-tracer experiments, and the advantages of the multitracer technique are presented.
authors.affiliation: Showa College of Pharmaceutical Sciences, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Hirunuma
authors.forename: K
authors.initials: K
authors.lastname: Endo
authors.forename: M
authors.initials: M
authors.lastname: Yanaga
authors.forename: S
authors.initials: S
authors.lastname: Enomoto
authors.forename: S
authors.initials: S
authors.lastname: Ambe
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Tozawa
authors.forename: F
authors.initials: F
authors.lastname: Ambe
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001693:Biological Transport, Active; D005069:Evaluation Studies as Topic; D008297:Male; D008722:Methods; D011868:Radioisotopes / Q000008:administration & dosage / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013051:Spectrometry, Gamma; D014018:Tissue Distribution; D014131:Trace Elements / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D011868:Radioisotopes; D014131:Trace Elements