An anti-adipocyte monoclonal antibody is cytotoxic to porcine preadipocytes in vitro and depresses the development of pig adipose tissue.
A mouse monoclonal antibody of the IgG2b subclass was raised against porcine adipocyte plasma membranes. This antibody did not cross-react in immunocytofluorescence with any tested cell-type or tissue other than porcine adipocytes. Complement-mediated cytotoxicity was demonstrated in primary cultures of porcine stromal-vascular cells. When the antibody and complement were added to already differentiated cultures, the treatment resulted in elimination of lipid-filled preadipocytes, whereas an early treatment of cultures prevented the appearance of these cells. In vivo, injection of newborn pigs with 1 mg/kg of monoclonal antibody on d 2 and 5 of life produced a more than 20% reduction of subcutaneous and leaf fat lipids at 35 d of age, whereas the lipid content of the longissimus muscle remained unaffected. These results demonstrate that early systemic treatment of pigs with a specific anti-adipocyte antibody reduces the fat mass. In addition to their potential in vivo use, monoclonal antibodies directed against adipose determinants may be useful tools for studying adipocyte lineage.
De clercq
L
L
Laboratoire de Biologie Cellulaire, Université Catholique de Louvain, Belgium.
Mourot
J
J
Genart
C
C
Davidts
V
V
Boone
C
C
Remacle
C
C
D017667:Adipocytes / Q000166:cytology* / Q000276:immunology* / Q000648:ultrastructure; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology / Q000502:physiology; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D000918:Antibody Specificity; D001835:Body Weight / Q000502:physiology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000502:physiology; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D051379:Mice; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D013552:Swine / Q000276:immunology / Q000502:physiology*; D015430:Weight Gain / Q000502:physiology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D008055:Lipids; D003165:Complement System Proteins