Activation of c-fos mRNA in the brain by the kappa-opioid receptor agonist enadoline and the NMDA receptor antagonist dizocilpine.
Using in situ hybridization, we have shown that the kappa-opioid receptor agonist enadoline (CI-977) and the non-competitive NMDA receptor antagonist dizocilpine (MK-901) induced the immediate early gene c-fos in dorsal medial thalamic nuclei. Dizocilpine, and not enadoline, also induced c-fos in the posterior cingulate cortex and retrosplenial cortex. Enadoline's stimulation of c-fos mRNA was dose and time dependent and completely inhibited by pretreatment with naltrexone. Morphine did not stimulate c-fos in the thalamus. It is suggested that the stimulation of c-fos with enadoline represents a specific kappa-opioid receptor effect.
Panegyres
P K
PK
Parke-Davis Neuroscience Research Centre, Cambridge University Forvie Site, UK. petepane@dunamis.rph.uwa.edu.au
Hughes
J
J
D000704:Analysis of Variance; D000818:Animals; D001345:Autoradiography; D001572:Benzofurans / Q000008:administration & dosage / Q000494:pharmacology*; D016291:Dizocilpine Maleate / Q000008:administration & dosage / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D017781:Genes, Immediate-Early / Q000187:drug effects / Q000235:genetics; D016762:Genes, fos / Q000187:drug effects* / Q000235:genetics; D017403:In Situ Hybridization; D007274:Injections, Intraperitoneal; D008297:Male; D011759:Pyrrolidines / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors*; D017473:Receptors, Opioid, kappa / Q000819:agonists*; D013787:Thalamic Nuclei / Q000187:drug effects / Q000378:metabolism
D001572:Benzofurans; D018691:Excitatory Amino Acid Antagonists; D011759:Pyrrolidines; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D017473:Receptors, Opioid, kappa; D016291:Dizocilpine Maleate; C067110:enadoline