Cotyledon thermal behavior and pectic solubility as related to cooking quality in common beans.
The characteristic of proteins, starch and pectic substances in cotyledons of two bean cultivars varying in cooking time were determined to investigate their possible contribution to bean cooking quality. Both cultivars showed the same enthalpies of starch gelatinization but different protein denaturation enthalpies. The proportion of hot water soluble pectins was higher in Michigan, the cultivar with the lower cooking time, than in Ojo de Cabra, the cultivar with the higher cooking time. These results were not due to differences in pectin methylation or in the ratio of monovalent to divalent cations in the tissue, suggesting that in fresh beans the beta-elimination reaction is not the sole or predominant route of thermal pectin degradation. Overall, this study indicates that varietal differences in bean cooking quality may be reflections of the rate of pectin loss during soaking/heating and that the thermal properties of starch and protein fractions seem to have a minor contribution. Researchers involved in this study propose that in fresh beans, the thermal pectin loss results from a two step mechanism: pectin enzymic breakdown during the bean soaking followed by thermal solubilization rather than beta-elimination during the bean heating.
Bernal-Lugo
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I
Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, México D.F.
Parra
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Portilla
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M
Peña-Valdivia
C B
CB
Moreno
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E
D000042:Absorption; D000688:Amylose / Q000032:analysis; D002152:Calorimetry, Differential Scanning; D002412:Cations / Q000032:analysis; D018548:Cotyledon / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry* / Q000145:classification; D005511:Food Handling; D006358:Hot Temperature; D010368:Pectins / Q000032:analysis / Q000737:chemistry*; D010946:Plants, Medicinal; D011506:Proteins / Q000032:analysis; D012995:Solubility; D013213:Starch / Q000032:analysis; D013997:Time Factors; D014867:Water / Q000737:chemistry
D002412:Cations; D011506:Proteins; D014867:Water; D010368:Pectins; D013213:Starch; D000688:Amylose