Immunohistochemical examination of the distribution of macrophages and CGRP-immunoreactive nerve fibers in induced rat periapical lesions.
To clarify the role of calcitonin gene-related peptide (CGRP) in the development of periapical lesions, we examined the distribution of CGRP-immunoreactive (IR) nerve fibers and macrophages, and the behavior of bone tissues in experimentally induced rat periapical lesions by immunohistochemical and quantitative methods. Although no extensive changes were observed at 7 days after pulp exposure, CGRP-IR nerve fibers increased in number until 28 days with a decrease thereafter. These neural changes were closely correlated with the alteration in number of macrophages except on day 7 when macrophages were significantly increased in number as compared with control rats. Tissue repair began to take place and a decrease in number of osteoclasts was observed when the density of CGRP-IR nerve fibers reached a peak. These results suggested that there might be a close relationship between macrophages and CGRP-IR nerve fibers and that CGRP-IR nerve fibers might participate in tissue repair in experimentally induced rat periapical lesions.
Toriya
Y
Y
Department of Conservative Dentistry 1, Kyushu University, Fukuoka, Japan.
Hashiguchi
I
I
Maeda
K
K
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D003782:Dental Pulp / Q000276:immunology / Q000294:innervation; D003790:Dental Pulp Necrosis / Q000150:complications; D007150:Immunohistochemistry; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000473:pathology*; D008297:Male; D009412:Nerve Fibers / Q000276:immunology / Q000473:pathology* / Q000502:physiology; D015213:Neuroimmunomodulation; D010485:Periapical Periodontitis / Q000209:etiology / Q000276:immunology* / Q000503:physiopathology*; D010486:Periapical Tissue / Q000737:chemistry / Q000276:immunology / Q000294:innervation*; D051381:Rats; D017208:Rats, Wistar
D015740:Calcitonin Gene-Related Peptide