Transposition of the Hermes element in embryos of the vector mosquito, Aedes aegypti.
Using a plasmid-based transpositional recombination assay in vivo, we have demonstrated that Hermes, a short inverted repeat type transposable element from Musca domestica, can transpose in Aedes aegypti embryos. Hermes transpositions in Ae. aegypti have all the characteristics observed during Hermes transposition in its host M. domestica and in related species. These characteristics include an absolute dependence on the expression of the Hermes transposase and a preference for the integration site GTNCAGAC (P < 0.05). In addition, the rate of Hermes transposition in Ae. aegypti (0.286 transpositions per 10,000 donor plasmids screened) was comparable to that observed in Drosophila melanogaster under similar conditions. These results suggest that Hermes can be developed into a gene vector and genetic engineering tool for Ae. aegypti and related mosquitoes.
Sarkar
A
A
Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park 20742, USA.
Yardley
K
K
Atkinson
P W
PW
James
A A
AA
O'Brochta
D A
DA
D000330:Aedes / Q000196:embryology / Q000235:genetics*; D000818:Animals; D004251:DNA Transposable Elements; D017344:Genes, Insect; D006793:Houseflies / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
D004251:DNA Transposable Elements