Further syntheses employing phosphorylase.
Maltopentaose was immobilized on silica gel and used as a recyclable primer in the reaction of glycogen phosphorylase with D-glucal. An improved method to obtain 2-deoxy-alpha-D-arabino-hexopyranosyl phosphate (4) by using this catalyst as well as the specific synthesis of low molecular weight, water-soluble 2-deoxy-maltooligosaccharides (12, 13, 14 and 15) are described. Further investigations with modified phosphorylase substrates showed that mannosyl phosphate (16) can be slowly transferred to the primer maltotetraose. alpha-1,4-Mannosyl-maltotetraose (17) and its degradation product alpha-1,4-mannosyl-maltose (18) were identified.
Evers
B
B
Institut für Organische Chemie, Universität Hamburg, Germany.
Thiem
J
J
D002240:Carbohydrate Sequence; D002384:Catalysis; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000378:metabolism; D008360:Mannosephosphates / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D006005:Phosphorylases / Q000378:metabolism*; D011198:Solanum tuberosum / Q000201:enzymology; D013379:Substrate Specificity; D013403:Sugar Phosphates / Q000096:biosynthesis
C090607:2-deoxy-arabino-hexopyranosyl phosphate; D008360:Mannosephosphates; D009844:Oligosaccharides; D013403:Sugar Phosphates; C001289:d-glucal; C019193:maltopentaose; D003847:Deoxyglucose; D006005:Phosphorylases