High-performance liquid chromatographic determination of myocardial interstitial dihydroxyphenylglycol.
This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) for monitoring dihydroxyphenylglycol (DHPG) in the myocardial interstitial space. Using a cardiac dialysis technique, 10-microl dialysates were sampled from the myocardial interstitial space (1-min fractions) and were injected directly into the HPLC-ED system. The in vitro quantification limit for DHPG was 250 fg in a 10-microl injection. The basal DHPG concentration of dialysate was 181+/-46 pg/ml. This system offers a new possibility for monitoring myocardial interstitial DHPG levels.
Takauchi
Y
Y
Department of Anesthesiology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.
Kitagawa
H
H
Kawada
T
T
Akiyama
T
T
Yamazaki
T
T
D000818:Animals; D002415:Cats; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D004563:Electrochemistry; D005110:Extracellular Space / Q000737:chemistry*; D005260:Female; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives* / Q000032:analysis; D009206:Myocardium / Q000737:chemistry*; D015203:Reproducibility of Results
D008734:Methoxyhydroxyphenylglycol; C010117:3,4-dihydroxyphenylglycol