Modulation of protein kinases and microtubule-associated proteins and changes in ultrastructure in female rat pituitary cells: effects of estrogen and bromocriptine.
This study focused on the intracellular signal transduction system and microtubule-associated proteins (MAPs), such as MAP-2 and Tau protein. The modulation of these proteins and their correlation with ultrastructural changes were investigated in rat pituitary prolactin (PRL) cells. Adult female Wistar rats were treated with estrogen and bromocriptine and their pituitary glands were removed for analysis of the expression of tubulin, MAP-2, Tau protein, protein kinase C (PKC), and calcium calmodulin (CaM) kinase. Western blot analysis showed that estrogen increased and bromocriptine decreased the expression of PKC alpha, beta 1, beta 2, CaM kinase alpha, beta, MAP-2, and Tau protein. MAP-2 and Tau protein, which are cytosolic proteins, being translated on free ribosomes, were associated with the membrane of whirling rough endoplasmic reticulum (RER) in estrogen-treated cells and dissociated with vesiculated RER induced by bromocriptine. These results suggested that the modulation of MAP-2 and Tau protein may reflect changes of PKC and CaM kinase, and that the quantitative changes and intracellular modulation of MAPs induced by estrogen and bromocriptine, i.e., estrogen-induced association and bromocriptine-induced dissociation of MAP-2 and Tau protein with membrane of RER, may reflect the dynamics of microtubules and are associated with structural changes in the RER and changes in the synthesis and intracellular transport of PRL.
Matsuno
A
A
Department of Neurosurgery, Teikyo University Ichihara Hospital, Chiba, Japan.
Takekoshi
S
S
Sanno
N
N
Utsunomiya
H
H
Ohsugi
Y
Y
Saito
N
N
Kanemitsu
H
H
Tamura
A
A
Nagashima
T
T
Osamura
R Y
RY
Watanabe
K
K
D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D010902:Pituitary Gland / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure*; D011388:Prolactin / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012270:Ribosomes / Q000378:metabolism; D015398:Signal Transduction; D016875:tau Proteins / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D016875:tau Proteins; D001971:Bromocriptine; D004958:Estradiol; D011388:Prolactin; D011494:Protein Kinases; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases