title: Optical imaging of low Mg(2+)-induced spontaneous epileptiform activity in combined rat entorhinal cortex-hippocampal slices.
abstract: A reproducible increase in transmission of infrared light was observed during spontaneous seizure-like events (SLEs) induced by low Mg2+ solutions in combined rat entorhinal cortex-hippocampal slices. Comparison of half maxima of transmission change in different regions indicated propagation of SLEs from the medial entorhinal cortex towards the temporal cortex suggesting spread along existing anatomical pathways. Thus, optical imaging of spontaneous epileptiform activity is possible and may improve the assessment of spread patterns. The optical signal outlasted both SLEs and associated K+ signals. In contrast, the tetraethylammonium signal, indicating changes of the extracellular space (ECS) volume, had a longer time course than the transmission changes. ECS volume changes are widely held to be responsible for transmission change. Our data suggest that other mechanisms may also contribute to increased light transmission during epileptiform activity.
authors.affiliation: Neurologische Klinik und Poliklinik, Universitätsklinikum Charité, Humboldt-Universität Berlin, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Meierkord
authors.forename: S
authors.initials: S
authors.lastname: Schuchmann
authors.forename: K
authors.initials: K
authors.lastname: Buchheim
authors.forename: U
authors.initials: U
authors.lastname: Heinemann
mesh_terms: D000818:Animals; D004594:Electrophysiology; D018728:Entorhinal Cortex / Q000473:pathology*; D004827:Epilepsy / Q000473:pathology*; D005110:Extracellular Space / Q000502:physiology; D019044:Image Cytometry; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D007259:Infrared Rays; D007700:Kinetics; D008275:Magnesium Deficiency / Q000473:pathology*; D011188:Potassium / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011188:Potassium