Carbohydrates on the surface of Echinococcus granulosus protoscoleces are immunodominant in mice.
A carbohydrate enriched soluble fraction (CHP) was prepared by mild treatment of viable Echinococcus granulosus protoscoleces (PSC) with the enzyme endoglycosidase-F (endo-F) and characterized by SDS-PAGE, glycoside- inhibition ELISA, and immunoblotting. Three groups of four BALB/c mice were immunized with the CHP, with the remaining deglycosylated PSC (DGP) and with dead PSC (DPSC) to analyse the relative immunogenicity of carbohydrates on the surface of PSC. A fourth sentinel group was not immunized. The antibody response was analyzed during primary and hyperimmune responses. Specific antibody titres (IgG and IgM) against somatic PSC antigens (PSA) were evaluated by ELISA and their antigen recognition pattern by immunoblotting, discriminating carbohydrate and peptide specific antibody responses by periodate treatment of PSA. The avidity index of those antibodies and the titer of non-specific immunoglobulins during the whole protocol were evaluated by ELISA in vitro mitogenic activity of CHP was also evaluated. The results indicated: 1) immunodominance of surface carbohydrate epitopes from PSC, 2) predominant IgM and low avidity response against these epitopes, 3) a dramatic increase of non-specific antibody titres and 4) in vitro mitogenic activity of CHP.
Míguez
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M
Cátedra de Inmunologia, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
Baz
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Nieto
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D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000915:Antibody Affinity; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000302:isolation & purification; D000954:Antigens, Surface / Q000302:isolation & purification; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D004443:Echinococcosis / Q000276:immunology; D004446:Echinococcus / Q000276:immunology*; D005260:Female; D006942:Hypergammaglobulinemia / Q000276:immunology; D007114:Immunization; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000302:isolation & purification; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D002241:Carbohydrates; D016056:Immunodominant Epitopes