Membrane modifications of red blood cells in Alzheimer's disease.
Red blood cells (RBC) from 24 Alzheimer's disease (AD) patients, 18 age- and sex-matched nondemented (ND) patients, hospitalized in the same facility for orthopedic problems, and 18 healthy volunteers aged 30-52 years were studied in order to gain insight into the nature of RBC membrane modifications in AD. Significant differences were found between RBC from AD and ND patients or young controls respectively for annexin V-binding (45.5 +/- 18.0% vs 27.1 +/- 14.7 and 2.7 +/- 1.9, p = .003), fraction of glycerol resistant cells (30.8 +/- 11.1% vs 19.6 +/- 6.4 and 10.2 +/- 3.1, p = .026), cell electrophoretic mobility in polymer (1.028 +/- 0.022 microns sec-1 V-1 cm vs 1.046 +/- 0.022 and 1.053 +/- 0.021, p = .02) and only limited significance for the filterability (1.46 +/- 0.12 msec vs 1.58 +/- 0.11 and 1.54 +/- 0.11, p = 0.1). A logistic analysis, using simultaneously several features as independent variables, suggested the combined use of annexinV- binding, glycerol resistance, and cell filterability which allowed the assignment of 95% of patients from this cohort to the right group. A prospective analysis of a larger cohort is required for the estimation of the diagnostic value of this test battery. In addition, the high level of annexin binding is characteristic of a disruption of the phospholipid asymmetry in aged or damaged cells, while the high glycerol resistance combined with low electrophoretic mobility an rigidity characterize young RBC, thus indicating an enhanced turnover of RBC in Alzheimer's disease.
Sabolovic
D
D
INSERM U313, CHU Pitié Salpétrière, Paris, France.
Roudier
M
M
Boynard
M
M
Pautou
C
C
Sestier
C
C
Fertil
B
B
Geldwerth
D
D
Roger
J
J
Pons
J N
JN
Amri
A
A
Halbreich
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood*; D017304:Annexin A5 / Q000097:blood; D015331:Cohort Studies; D004351:Drug Resistance; D004586:Electrophoresis; D004907:Erythrocyte Deformability; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000502:physiology*; D004912:Erythrocytes / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D008875:Middle Aged; D011108:Polymers; D012016:Reference Values
D017304:Annexin A5; D011108:Polymers; D005990:Glycerol