Determination of acetaldehyde in biological samples by gas chromatography with electron-capture detection.
A simple specific assay was developed for the determination of acetaldehyde in biological samples. Acetaldehyde was derivatized to 2,4-dinitrophenylhydrazone, which was determined by gas chromatography with electron-capture detection. The use of this detection method is an important device to which no one drew notice. This procedure was very simple and so sensitive that as little as 500 fmol of acetaldehyde could be measured in aqueous solution. The calibration curve of acetaldehyde was linear at least up to 40 microM. Its recoveries from human plasma and rat liver homogenate were 96.5 and 95.7%, respectively.
Ohata
H
H
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Otsuka
M
M
Ohmori
S
S
D000079:Acetaldehyde / Q000032:analysis* / Q000097:blood; D000818:Animals; D002849:Chromatography, Gas; D000431:Ethanol / Q000493:pharmacokinetics; D005947:Glucose / Q000032:analysis; D006801:Humans; D006835:Hydrazones; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
C001072:2,4-dinitrophenylhydrazone; D006835:Hydrazones; D000431:Ethanol; D000079:Acetaldehyde; D005947:Glucose