Evaluation of tests for detection of antibodies to Aujeszky's disease (pseudorabies) virus glycoprotein E in the target population.
Receiver operating characteristic (ROC) curves assess the quality of tests over the entire range of test signals. We compared the ability of an ELISA to detect antibodies to Aujeszky's disease (pseudorabies) virus gE in colostrum (test A) and in a single droplet of whole blood (test B) with the results obtained in serum (gold standard) in the target population by constructing and analyzing such curves. The area under the ROC curve, which is a quantitative measure of test performance, proved to be significantly (p < 0.01) smaller in test A than in test B or the gold standard. No significant differences in the area under the ROC curve were observed between test B and the gold standard.
Stegeman
A
A
Animal Health Service, Boxtel, The Netherlands. j.a.stegeman@id.dlo.nl
De Jong
M C
MC
Van der Heijden
H M
HM
Elbers
A R
AR
Kimman
T G
TG
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D003126:Colostrum / Q000276:immunology; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D011557:Pseudorabies / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D022421:Pseudorabies Vaccines; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines
D000914:Antibodies, Viral; D022421:Pseudorabies Vaccines; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C089580:glycoprotein E, Suid herpesvirus 1