Modulation of macrophage inflammatory protein-1alpha and its receptors in human B-cell lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma.
Macrophage inflammatory protein-1 alpha (MIP-1alpha) is a member of the -C-C- family of low-molecular weight chemokines. MIP-1alpha is involved in the homeostatic control of stem cell proliferation, in inducing chemotaxis, and also in inflammatory responses in mature cell types. In order to observe modulations of MIP-1alpha secretion and expression along with MIP-1alpha receptor (MIP-1alpha-R) expression for a possible autocrine role in AIDS associated B-cell lines, we studied a wide panel of human B-cell lines. Previous work by us has shown that HIV-1 tat down modulates MIP-1alpha by inducing a novel transcription factor MNP. Our data in this report suggest that HIV down modulates MIP-1alpha as a mechanism to evade suppression by this chemokine in human B-cells. Furthermore, our results strongly suggest MIP-1alpha autocrine loops in a majority of tumor B-cells as evident by MIP-1alpha-R expression, and also secretion of MIP-1alpha.
Sharma
V
V
Department of Biology, University of West Florida, Pensacola 32514, USA. vsharma@nautilus.uwf.edu
Walper
D
D
Deckert
R
R
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002051:Burkitt Lymphoma / Q000276:immunology*; D002460:Cell Line; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D003001:Cloning, Molecular; D017931:DNA Primers; D006801:Humans; D007700:Kinetics; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis* / Q000737:chemistry; D016133:Polymerase Chain Reaction; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D017931:DNA Primers; D019402:Macrophage Inflammatory Proteins; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D011994:Recombinant Proteins; C080633:macrophage inflammatory protein 1alpha receptor; D013755:Tetradecanoylphorbol Acetate