Establishment and characterization of 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat erythroleukemia cell lines.
To investigate molecular mechanisms and biological behaviors of 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat erythroleukemia, we established 8 new culture cell lines from 7 primary erythroleukemias. We designated them KYD-10, 12, 17, 32, 38, 44A, 44B, and 49. Representative clones isolated from each cell line in early passage were analyzed cytogenetically and genetically. All cell lines except KYD-12 possessed the specific N-ras mutation at the 2nd base of codon 61. Four of them showed #2 trisomy (KYD-10, 32, 38, 44B), and the rest normal diploid karyotype (2n). KYD-32 cells showed Robertson type II #2 trisomy which had never been clonally isolated in vitro although it was reported in some DMBA leukemias in vivo. We further studied the genomic imbalance related to the N-ras allele using mutant-allele-specific amplification (MASA) method. Deletion of normal N-ras allele was found in 5 of 8 cell lines. KYD-32 and 38 retained the normal N-ras allele. The specific N-ras mutation and allelic loss of wild type N-ras were correlated with advanced cell proliferation in culture probably independent of #2 trisomy.
Koh
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T
Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Japan.
Osaka
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M
Sugiyama
T
T
D015127:9,10-Dimethyl-1,2-benzanthracene; D000483:Alleles; D000818:Animals; D002460:Cell Line; D016259:Genes, myc; D011905:Genes, ras; D007621:Karyotyping; D004915:Leukemia, Erythroblastic, Acute / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D017354:Point Mutation; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D051381:Rats; D011917:Rats, Inbred Lew; D014314:Trisomy; D014407:Tumor Cells, Cultured
D016271:Proto-Oncogene Proteins c-myc; D015127:9,10-Dimethyl-1,2-benzanthracene