Lack of phospholipase A2 mutations in neuroblastoma, melanoma and colon-cancer cell lines.
A candidate murine tumor-suppressor gene, Mom1, has been identified as the secretory phospholipase A2 (GDB nomenclature: PLA2G2A) gene. Evidence suggests that PLA2G2A functions as a tumor-suppressor because mice lacking PLA2G2A expression demonstrate increased colonic polyposis. The human homologue of PLA2G2A has been mapped to chromosome 1p36, a region frequently implicated in the pathogenesis of neuroblastoma, colon cancer and melanoma. We identified 2 alterations in the PLA2G2A gene in a single neuroblastoma cell line out of 20 examined; however, we found no mutations in 24 melanoma cell lines, 12 lymphoblastoid cell lines from patients having chromosome 1-linked familial melanoma and 10 colon cancer cell lines. Secretory phospholipase A2 is unlikely to play a significant role in the pathogenesis of these tumors.
Haluska
F G
FG
Department of Hematology/Oncology, Massachusetts General Hospital, Boston 02114, USA. HALUSKA.FRANK@MGH.HARVARD.EDU
Thiele
C
C
Goldstein
A
A
Tsao
H
H
Benoit
E P
EP
Housman
D
D
D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003110:Colonic Neoplasms / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D051379:Mice; D009154:Mutation; D009447:Neuroblastoma / Q000235:genetics*; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D014407:Tumor Cells, Cultured
D010741:Phospholipases A; D054467:Phospholipases A2