The application of papain, ficin and clostripain in kinetically controlled peptide synthesis in frozen aqueous solutions.
The capability of the cysteine proteases ficin, papain and clostripain to form peptide bonds in frozen aqueous solutions was investigated. Freezing the reaction mixture resulted in increased peptide yields in kinetically controlled coupling of Bz-Arg-OEt with various amino acid amides and dipeptides. Under these conditions, peptide yields increased up to 70% depending on the enzyme and the amino component used. Enzyme-catalysed peptide syntheses were carried out under optimized reaction conditions (temperature, amino component concentration and pH before freezing) using the condensation of Bz-Arg-OEt and H-Leu-NH2 as a model reaction.
Hänsler
M
M
Faculty of Biosciences, Pharmacy and Psychology, Institute of Biochemistry, Leipzig University, Germany.
Ullmann
G
G
Jakubke
H D
HD
D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004151:Dipeptides / Q000096:biosynthesis / Q000737:chemistry; D005361:Ficain / Q000378:metabolism*; D007053:Ice; D007202:Indicators and Reagents; D007700:Kinetics; D015394:Molecular Structure; D010206:Papain / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry; D012996:Solutions
D004151:Dipeptides; D007053:Ice; D007202:Indicators and Reagents; D010455:Peptides; D012996:Solutions; D003546:Cysteine Endopeptidases; D010206:Papain; D005361:Ficain; C019913:clostripain