title: AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location.
abstract: The AMP-activated protein kinase (AMPK) consists of catalytic alpha and non-catalytic, beta and gamma (38 kDa) subunits and is responsible for acting as a metabolic sensor for AMP levels. There are multiple genes for each subunit and we find that rat liver AMPK-alpha2 isoform catalytic subunit is associated with beta1 and gamma1 and not with beta2 or gamma2 subunit isoforms. The beta1 and gamma1 isoforms are also subunits of the alpha1 isoform. The sequence of cloned human AMPK-beta1 is 95% identical in amino acid sequence with rat beta1. Human chromosomal localizations were determined for AMPK-alpha1 (5p11-p14), AMPK-beta1 (12q24.1-24.3) and AMPK-gamma1 (12q12-q14), respectively.
authors.affiliation: St. Vincent's Institute of Medical Research, St. Vincent's Hospital, Fitzroy, Victoria, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Stapleton
authors.forename: E
authors.initials: E
authors.lastname: Woollatt
authors.forename: K I
authors.initials: KI
authors.lastname: Mitchelhill
authors.forename: J K
authors.initials: JK
authors.lastname: Nicholl
authors.forename: C S
authors.initials: CS
authors.lastname: Fernandez
authors.forename: B J
authors.initials: BJ
authors.lastname: Michell
authors.forename: L A
authors.initials: LA
authors.lastname: Witters
authors.forename: D A
authors.initials: DA
authors.lastname: Power
authors.forename: G R
authors.initials: GR
authors.lastname: Sutherland
authors.forename: B E
authors.initials: BE
authors.lastname: Kemp
mesh_terms: D055372:AMP-Activated Protein Kinases; D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002895:Chromosomes, Human, Pair 5; D002897:Chromosomes, Human, Pair 7; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D008297:Male; D008677:Metaphase / Q000235:genetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000235:genetics*; D005810:Multigene Family; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D051381:Rats
chemical_list: D007527:Isoenzymes; D009097:Multienzyme Complexes; D011494:Protein Kinases; C526181:PRKAA2 protein, human; C526182:PRKAB1 protein, human; C526183:PRKAB2 protein, human; C526222:PRKAG1 protein, human; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; C526180:PRKAA1 protein, human