title: Effects of lysosomal protease inhibitors on the degradation of acetylated low density lipoprotein in cultured rat peritoneal macrophages.
abstract: The effect of protease inhibitors, leupeptin and pepstatin A, on the metabolism of acetylated low density lipoprotein (acetyl-LDL) was investigated in cultured rat peritoneal macrophages and compared with that of chloroquine. While both leupeptin and pepstatin inhibited the proteolytic degradation of 125I-acetyl-LDL, a combination of both showed an additive effect. Similar to chloroquine, both protease inhibitors diminished [3H] oleate incorporation into cellular cholesteryl[3H] oleate and increased cholesterol content of macrophages. These results suggest that both thiol protease and cathepsin D participate in the physiological degradation of apolipoprotein in macrophages. The inhibition of apolipoprotein degradation seemed to have an effect on cholesterol metabolism in macrophages cultured with acetyl-LDL.
authors.affiliation: Second Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kuroda
authors.forename: M
authors.initials: M
authors.lastname: Yoshinari
authors.forename: K
authors.initials: K
authors.lastname: Okamura
authors.forename: K
authors.initials: K
authors.lastname: Okazawa
authors.forename: H
authors.initials: H
authors.lastname: Ikenoue
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Fujishima
mesh_terms: D000107:Acetylation; D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D007976:Leupeptins / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000187:drug effects* / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects* / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism; D010436:Pepstatins / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D014316:Tritium
chemical_list: D007457:Iodine Radioisotopes; D007976:Leupeptins; D008077:Lipoproteins, LDL; D010436:Pepstatins; D011480:Protease Inhibitors; D014316:Tritium; C014195:Streptomyces pepsin inhibitor; D019301:Oleic Acid; D002784:Cholesterol; C032854:leupeptin; C031375:pepstatin