title: Cloning and gene mapping of the chromosome 13q14 region deleted in chronic lymphocytic leukemia.
abstract: Frequent deletions and loss of heterozygosity in a segment of chromosome 13 (13q14) in cases of B-cell chronic lymphocytic leukemia (CLL) have suggested that this malignancy is caused by inactivation of an unknown tumor suppressor gene located in this region. Toward the identification of the putative CLL tumor suppressor, we have constructed a high-resolution physical map of YAC, PAC, and cosmid contigs covering 600 kb of the 13q14 genomic region. In addition to densely positioned genetic markers and STSs, this map was further annotated by localization of 32 transcribed sequences (ESTs) using a combination of exon trapping, direct cDNA selection, sample sequencing of cosmids and PACs, and homology searches. On the basis of these mapping data, allelic loss analyses at 13q14 using CLL tumor samples allowed narrowing of the genomic segment encompassing the putative CLL gene to <300 kb. Twenty-three ESTs located within this minimally deleted region are candidate exons for the CLL-associated tumor suppressor gene.
authors.affiliation: Columbia Genome Center, Columbia University, New York, New York 10032, USA. kalachikov@cuccfa.ccc.columbia.edu
authors.forename: S
authors.initials: S
authors.lastname: Kalachikov
authors.forename: A
authors.initials: A
authors.lastname: Migliazza
authors.forename: E
authors.initials: E
authors.lastname: Cayanis
authors.forename: N S
authors.initials: NS
authors.lastname: Fracchiolla
authors.forename: M F
authors.initials: MF
authors.lastname: Bonaldo
authors.forename: L
authors.initials: L
authors.lastname: Lawton
authors.forename: P
authors.initials: P
authors.lastname: Jelenc
authors.forename: X
authors.initials: X
authors.lastname: Ye
authors.forename: X
authors.initials: X
authors.lastname: Qu
authors.forename: M
authors.initials: M
authors.lastname: Chien
authors.forename: R
authors.initials: R
authors.lastname: Hauptschein
authors.forename: G
authors.initials: G
authors.lastname: Gaidano
authors.forename: U
authors.initials: U
authors.lastname: Vitolo
authors.forename: G
authors.initials: G
authors.lastname: Saglio
authors.forename: L
authors.initials: L
authors.lastname: Resegotti
authors.forename: V
authors.initials: V
authors.lastname: Brodjansky
authors.forename: N
authors.initials: N
authors.lastname: Yankovsky
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: M B
authors.initials: MB
authors.lastname: Soares
authors.forename: J
authors.initials: J
authors.lastname: Russo
authors.forename: I S
authors.initials: IS
authors.lastname: Edelman
authors.forename: A
authors.initials: A
authors.lastname: Efstratiadis
authors.forename: R
authors.initials: R
authors.lastname: Dalla-Favera
authors.forename: S G
authors.initials: SG
authors.lastname: Fischer
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA; D006801:Humans; D006822:Hybrid Cells; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
chemical_list: D004247:DNA