Cloning and subcellular localization of hamster and rat isopentenyl diphosphate dimethylallyl diphosphate isomerase. A PTS1 motif targets the enzyme to peroxisomes.
To date, isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase; EC 5.3.3.2) is presumed to have a cytosolic localization. However, we have recently shown that in permeabilized cells lacking cytosolic components, mevalonate can be converted to cholesterol, implying that all of the enzymes required for the conversion of mevalonate to farnesyl diphosphate are found in the peroxisome. To provide unequivocal evidence for the subcellular localization of IPP isomerase, in this study, we have cloned the rat and hamster homologues of IPP isomerase and identified the signal that targets this enzyme to peroxisomes. In addition, we also demonstrate that IPP isomerase is regulated at the mRNA level.
Paton
V G
VG
Department of Biology, San Diego State University, San Diego, California 92182, USA.
Shackelford
J E
JE
Krisans
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D015139:Blotting, Southern; D019748:Carbon-Carbon Double Bond Isomerases; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis; D003001:Cloning, Molecular; D016384:Consensus Sequence; D006224:Cricetinae; D004044:Dietary Proteins / Q000378:metabolism; D045782:Hemiterpenes; D016045:Human Genome Project; D006801:Humans; D007535:Isomerases / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000201:enzymology; D008297:Male; D008798:Mevalonic Acid / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D000074435:Peroxisome-Targeting Signal 1 Receptor; D021382:Protein Sorting Signals / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism
D004044:Dietary Proteins; D045782:Hemiterpenes; D000074435:Peroxisome-Targeting Signal 1 Receptor; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D002784:Cholesterol; D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase; D008798:Mevalonic Acid