Absence of a persistently elevated 37 kDa fos-related antigen and AP-1-like DNA-binding activity in the brains of kainic acid-treated fosB null mice.
Chronic stimulation of the nervous system or acute administration of kainic acid results in a persistent increase in AP-1-like DNA-binding activity in the brain. However, the composition and function of these AP-1 complexes remain controversial. By comparing wild-type and fosB-null mice treated with kainic acid, we establish that the complexes comprise JunD in association with an approximately 37 kDa Delta-FosB species. Delta-FosB was expressed persistently in neurons in many areas of the CNS, even though fosB mRNA only increased transiently. This implies that the 37 kDa protein is very stable. fosB-/- mice are predisposed to seizures. Therefore, the chronic expression of Delta-FosB elicited by kainic acid seizures may be indicative of a compensatory/protective role in the pathophysiology of epilepsy.
Mandelzys
A
A
Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Gruda
M A
MA
Bravo
R
R
Morgan
J I
JI
D000818:Animals; D001324:Autoantigens / Q000378:metabolism*; D001921:Brain / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D017403:In Situ Hybridization; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017207:Rats, Sprague-Dawley; D018808:Transcription Factor AP-1 / Q000378:metabolism*
D001324:Autoantigens; D004268:DNA-Binding Proteins; D018808:Transcription Factor AP-1; C089731:islet 37k antigen; D007608:Kainic Acid