Substitution of alanine for aspartate at position 179 in the SHV-6 extended-spectrum beta-lactamase.
SHV-6 was previously identified by its susceptibility pattern and biochemical criteria in a clinical isolate of Klebsiella pneumoniae which was resistant to ceftazidime. It contains only a single point difference with the beta laSHV-1 gene as determined by PCR amplification and nucleotide sequencing. This is the result of a single amino acid substitution, Ala for Asp, at position 179. Directed mutagenesis experiments have shown this substitution to confer selective resistance to ceftazidime in the TEM family.
Arlet
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G
Université Paris VII Denis Diderot, Service de Microbiologie, Hôpital Saint-Louis, Paris, France.
Rouveau
M
M
Philippon
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002442:Ceftazidime / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D013329:Structure-Activity Relationship; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology
D002442:Ceftazidime; D001618:beta-Lactamases