Proteasome and myogenesis.
In this report, we examine the involvement of the ubiquitin-proteasome pathway during fusion and differentiation of myoblast primary cell cultures. Up-regulation of proteasome was observed at the maximum fusion rate and was preceded by an increase of unidentified ubiquitin-conjugates. Cell permeable proteasome inhibitors prevent fusion as do antisense oligodesoxyribonucleotides targetted to three proteasome subunits. Identical results were obtained using E3 ubiquitin-ligases dipeptide inhibitor. Involvement of the ubiquitin-proteasome pathway in the regulation of myogenic factors was hypothesized.
Gardrat
F
F
ENSSTAB, Laboratoire de Biochimie et Technologie des Aliments URA-INRA, Université Bordeaux 1, Talence, France.
Montel
V
V
Raymond
J
J
Azanza
J L
JL
D000818:Animals; D002454:Cell Differentiation; D002459:Cell Fusion; D003546:Cysteine Endopeptidases / Q000502:physiology*; D009097:Multienzyme Complexes / Q000502:physiology*; D018482:Muscle, Skeletal / Q000196:embryology*; D046988:Proteasome Endopeptidase Complex; D051381:Rats; D014452:Ubiquitins / Q000378:metabolism
D009097:Multienzyme Complexes; D014452:Ubiquitins; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex