[Expression of cDNA of human chorionic gonadotropin beta-subunit (beta-hCG) cDNA in insect cells and effect of expressed product on mouse lymphocytes in vitro].
Expression vector pVL 1393-hCG beta containing beta-hCG cDNA has been constructed using an unfused protein nuclear polyhedrosis virus (AcNPV) expression vector. The insect cells (Sf 9) were cotransfected by the expression vector and nuclear polyhedrosis virus genomic DNA, and recombinant virus AcNPV-hCG beta was screened out, beta-hCG cDNA was expressed in insect cells infected by recombinant virus and recombinant beta-hCG (r beta-hCG) was secreted into medium. The purity of r beta-hCG, purified by immuno-affinity chromatography, was about 90% and the molecular weight of r beta-hCG was 22,500 Da. Like hCG, r beta-hCG suppressed significantly proliferation of induced lymphocytes, as well as production of IL-2 to some extent, on a parallel with suppression of lymphoproliferation.
Shen
Q X
QX
Shanghai Institute of Cell Biology, Academia Sinica, Shanghai.
Li
C L
CL
Shen
H
H
Liu
H H
HH
Xiang
C Q
CQ
Ding
X C
XC
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000235:genetics* / Q000494:pharmacology; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D014162:Transfection
D018997:Chorionic Gonadotropin, beta Subunit, Human; D018076:DNA, Complementary; D007376:Interleukin-2; D011993:Recombinant Fusion Proteins