title: [The study of radioimmunoimaging on the nude mice bearing human ovarian carcinoma by labelling monoclonal antibody with 99mTc].
abstract: OBJECTIVE
  To investigate the feasibility of radioimmunoimaging (RII) on the nude mice model by labelling anti-ovarian carcinoma monoclonal antibody (McAb, COC166-9) with 99mTc, and the possibility for clinical application.
  
  
  METHODS
  McAb reduced by beta-mercaptoethanol was labelled directly with 99mTc. Imaging positive rate, negative rate and tumor/non-tumor (T/N) ratio were calculated.
  
  
  RESULTS
  (1)McAb COC166-9 labelled with 99mTc reacted with ovarian carcinoma. (2) 8 nude mice models were injected with the labelled antibodies and were then imaged. In the experimental group the positive imaging rate of tumor was 100%, in the control group the negative rate was 100%. The results were the same as those of T/N ratios. (3)In two groups (10 nude mice), intraperitoneal (i.p.) or intravenous (i.v.) injection of McAb labelled with 99mTc, positive imaging rates of tumor 100%. The IV group was better than IP group in the radio-background.
  
  
  CONCLUSIONS
  Immunoactivity of 99mTc-COC166-9 used for RII on the nude mice models was good, and the imaging effect was specific. Clinical application is promising.
authors.affiliation: People's Hospital, Beijing Medical University.
authors.forename: W
authors.initials: W
authors.lastname: Lin
authors.forename: H
authors.initials: H
authors.lastname: Qian
authors.forename: J
authors.initials: J
authors.lastname: Feng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D016719:Radioimmunodetection; D013667:Technetium; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D013667:Technetium