title: Determination of endogenous peptides with in vitro ACE inhibitory activity in normotensive human plasma by the fluorometric HPLC method.
abstract: An in vitro degradation test of angiotensin (ANG) II or III in normotensive supine human plasma from 9 healthy male subjects confirmed the production of smaller ANG metabolites with angiotensin I-converting enzyme inhibitory activity. These metabolites were identified as ANG (3-8), ANG (5-8), and ANG (3-4), whose respective peptide concentrations were determined by our proposed naphthalene-2,3-dialdehyde (NDA)-HPLC method to be 64 +/- 9, 39 +/- 5, 176 +/- 22, and 197 +/- 35 fmol/ml of plasma.
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsui
authors.forename: H
authors.initials: H
authors.lastname: Matsufuji
authors.forename: T
authors.initials: T
authors.lastname: Kawasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Osajima
mesh_terms: D000328:Adult; D000804:Angiotensin II / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D000805:Angiotensin III / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents / Q000737:chemistry; D008297:Male; D009281:Naphthalenes / Q000737:chemistry; D010455:Peptides / Q000097:blood*; D011863:Radioimmunoassay; D013050:Spectrometry, Fluorescence; D016683:Supine Position
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D007202:Indicators and Reagents; D009281:Naphthalenes; D010455:Peptides; D000804:Angiotensin II; D000805:Angiotensin III; C027717:2,3-naphthalenedicarboxaldehyde