Infrequent alterations of the p16 (MTS-1) gene in human gastric cancer.
p16 (MTS-1, multiple tumor suppressor gene 1), a putative tumor suppressor gene, is one of the cyclin-dependent kinase inhibitors (CDI) and it regulates the G1/S transition of the cell cycle. To clarify the role of p16 in primary gastric cancer, we have investigated somatic mutations of this gene by using the polymerase chain reaction/single strand conformation polymorphism (PCR-SSCP) method. In 23 surgical specimens of primary gastric cancer, none were detected in exon1 and exon 2. Among the 6 human gastric cancer cell lines examined, PCR products were not found in 2, MKN28 and MKN45, suggesting the presence of homozygous deletions. No mutation was found in the other 4 cell lines. Furthermore, decreased expression levels were not observed in 13 gastric cancer tissues by reverse transcription PCR (RT-PCR). Considering the above results of PCR-SSCP and RT-PCR, genetic alterations of the p16 gene are rarely implicated in human gastric cancer tumorigenesis.
Takaoka
A S
AS
First Department of Internal Medicine, Sapporo Medical University, Japan.
Kakiuchi
H
H
Itoh
F
F
Hinoda
Y
Y
Kusano
M
M
Ohara
M
M
Tsukakoshi
H
H
Hosokawa
M
M
Imai
K
K
D000199:Actins / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis*; D002460:Cell Line; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016147:Genes, Tumor Suppressor; D006801:Humans; D016133:Polymerase Chain Reaction; D047428:Protein Kinase Inhibitors; D013270:Stomach / Q000737:chemistry; D013274:Stomach Neoplasms / Q000737:chemistry / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000199:Actins; D002352:Carrier Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D047428:Protein Kinase Inhibitors