Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae.
The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher than 25 degrees C, it catalyzes acyl transfer reactions on properly structured thiolesters, and it binds penicillin with high affinity.
Lepage
S
S
Centre d'Ingénierie des Protéines, Institut de Chimie, Université dede Liège, Belgium.
Dubois
P
P
Ghosh
T K
TK
Joris
B
B
Mahapatra
S
S
Kundu
M
M
Basu
J
J
Chakrabarti
P
P
Cole
S T
ST
Nguyen-Distèche
M
M
Ghuysen
J M
JM
D000215:Acylation; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003360:Cosmids; D004926:Escherichia coli / Q000235:genetics; D006602:Hexosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002267:Muramoylpentapeptide Carboxypeptidase; D009166:Mycobacterium leprae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000378:metabolism*; D010458:Peptidyl Transferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013696:Temperature; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D011994:Recombinant Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase