Decay of calcium transients after electrical stimulation in rat fast- and slow-twitch skeletal muscle fibres.
1. Calcium transients were calculated from fura-2 fluorescence signals (corrected for kinetic delays in the Ca(2+)-fura-2 reaction) from single rat skeletal muscle fibres, either fully dissociated from the fast-twitch flexor digitorum brevis (FDB) muscle or in small bundles from the slow-twitch soleus muscle. Fibres or bundles were embedded in agarose gel to inhibit movement and stimulated by single or trains of 1-2 ms electrical pulses (100 Hz, 2-400 ms train duration). 2. The rate constant of decay of [Ca2+] determined from single-exponential fits to the final decay phase of [Ca2+] after a single action potential was considerably faster in FDB fibres than in soleus fibres. As the stimulation duration increased, the rate constant of [Ca2+] decay decreased for both the FDB and soleus fibres, but the effect was greater in FDB than in soleus fibres. 3. Using the magnitude of the decline in the rate constant of [Ca2+] decay with increasing stimulation duration as an index of relative contribution of the saturable Ca2+ binding sites on parvalbumin, subpopulations termed 'high', 'medium' and 'low', referring to estimated parvalbumin content, were determined within each group of FDB and soleus fibres. In fibres assigned to the 'high' and 'medium' groups, parvalbumin was the major contributor (50-73%) to the [Ca2+] decay rate constant after a single action potential. In fibres in the 'low' group, parvalbumin contributed only 0-28% to the rate constant of [Ca2+] decay. 4. Fluorescence recordings using mag-fura-2, a lower-affinity Ca2+ indicator expected to be in equilibrium with myoplasmic Ca2+, gave similar values for both the [Ca2+] decay rate constant after a single action potential and the decrease in this rate constant with increased stimulation duration, as found for the fura-2 [Ca2+] transients from FDB and soleus fibres. Thus, the observed differences in decay rate of Ca2+ were not introduced by kinetic correction of the fura-2 recordings, but are attributed to differences in the Ca2+ binding and transport properties of fast- and slow-twitch mammalian fibres.
Carroll
S L
SL
University of Maryland, School of Medicine, Department of Biochemistry and Molecular Biology, Baltimore 21201, USA.
Klein
M G
MG
Schneider
M F
MF
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004558:Electric Stimulation; D005260:Female; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D066298:In Vitro Techniques; D017136:Ion Transport; D007700:Kinetics; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism*; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism; D051381:Rats
D005456:Fluorescent Dyes; D010320:Parvalbumins; C058776:2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid; D000252:Calcium-Transporting ATPases; D002118:Calcium; D016257:Fura-2