title: A transcript map encompassing the multiple endocrine neoplasia type-1 (MEN1) locus on chromosome 11q13.
abstract: A transcription map of a 1200-kb region encompassing the MEN1 locus was constructed by direct cDNA selection and mapping ESTs. A total of 29 genes were mapped. Ten transcripts were identified by cDNA selection of a focused 300-kb genomic region telomeric to the MEN1 consensus region. Since many of the sequences cloned by cDNA selection also identified ESTs from the region, 19 additional RH-mapped ESTs were mapped to the entire contig region by PCR amplification of genomic clones. Nine known genes, 2 putative human homologues to mouse genes, and 18 novel transcripts map to the region. Transcripts that map to the MEN1 interval PYGM-D11S449 include SGC35223, IB1256, AA147620, ZFM1, FAU, and CAPN1. The latter 3 known genes have already been excluded as candidate MEN1 genes. The 2 putative human homologues of mouse genes Ltbp2 and Spa-1 may be candidate tumor suppressor genes, but they map telomeric to D11S449. Although both of these genes map outside the MEN1 consensus region they may play a role in sporadic endocrine tumors independent of the MEN1 gene or in other tumors, such as breast cancer, that have loss of heterozygosity within this region.
authors.affiliation: Department of Surgery Molecular Biology Core Unit, West Los Angeles Veterans Administration Medical Center, California 90073, USA. msawicki@ucla.edu
authors.forename: M
authors.initials: M
authors.lastname: Sawicki
authors.forename: E
authors.initials: E
authors.lastname: Arnold
authors.forename: S
authors.initials: S
authors.lastname: Ebrahimi
authors.forename: T
authors.initials: T
authors.lastname: Duell
authors.forename: S
authors.initials: S
authors.lastname: Jin
authors.forename: T
authors.initials: T
authors.lastname: Wood
authors.forename: R
authors.initials: R
authors.lastname: Chakrabarti
authors.forename: J
authors.initials: J
authors.lastname: Peters
authors.forename: Y
authors.initials: Y
authors.lastname: Wan
authors.forename: G
authors.initials: G
authors.lastname: Samara
authors.forename: H U
authors.initials: HU
authors.lastname: Weier
authors.forename: N
authors.initials: N
authors.lastname: Udar
authors.forename: E
authors.initials: E
authors.lastname: Passaro
authors.forename: E S
authors.initials: ES
authors.lastname: Srivatsan
mesh_terms: D015152:Blotting, Northern; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018761:Multiple Endocrine Neoplasia Type 1 / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: C105373:MEN1 protein, human; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins