title: Inactivation of Bacillus spores by the supercritical carbon dioxide micro-bubble method.
abstract: Bacillus spores were effectively inactivated by the supercritical (SC) CO2 micro-bubble method. The micro-bubble SC CO2 treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40 degrees C and 30 MPa for 30 min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO2 treatment of B. polymyxa, B. cereus, and B. subtilis spores at 45 degrees C, 50 degrees C, respectively, and 30 MPa for 60 min resulted in a 6-log cycle reduction of survival. The SC CO2 treatment under the foregoing conditions should offer higher efficiency than that of heat treatment at 100 degrees C for 60 min. In addition, the SC CO2 treatment (30 MPa, 60 degrees C, 30 min) of B. polymyxa and B. cereus spores also produced a 6-log cycle reduction.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Shimoda
authors.forename: K
authors.initials: K
authors.lastname: Tamaya
authors.forename: A
authors.initials: A
authors.lastname: Yonekura
authors.forename: T
authors.initials: T
authors.lastname: Kawano
authors.forename: Y
authors.initials: Y
authors.lastname: Osajima
mesh_terms: D001407:Bacillus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002245:Carbon Dioxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D011312:Pressure; D013171:Spores, Bacterial / Q000187:drug effects; D013242:Sterilization / Q000379:methods* / Q000592:standards; D013696:Temperature
chemical_list: D002245:Carbon Dioxide