title: Enzymatic treatment to enhance carotenoid content in dehydrated marigold flower meal.
abstract: The effect of enzymatic treatments using a commercial enzyme (Econase-cep at pH 5.0 and 0.1% w/w concentration) at different levels of dehydrated marigold meal (5, 10, 15 and 20% dry weight), to enhance carotenoid extraction, was evaluated. The AOAC method consisting of a simultaneous extraction and saponification by using hexane-ethanol-acetone-toluene (10:6:7:7 v/v) and metanolic KOH (40% w/v) was used to evaluate the carotenoid content. The measurement of carotenoids was also carried out in samples in which the water soluble compounds were previously eliminated (AOAC-H2O). Total carotenoids ranged from 11.4 to 17.4 g/kg and 18 to 24.7 g/kg of control and treated marigold meal, respectively. Highest amount of carotenoids were noted when 5% level of treated meal was used.
authors.affiliation: Departmento de Biotecnologia y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Trapuato, Gio. México.
authors.forename: F
authors.initials: F
authors.lastname: Delgado-Vargas
authors.forename: O
authors.initials: O
authors.lastname: Paredes-López
mesh_terms: D002338:Carotenoids / Q000032:analysis* / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism; D013997:Time Factors
chemical_list: D002338:Carotenoids; D005959:Glucosidases; D006026:Glycoside Hydrolases; C023305:hemicellulase; D011096:Polygalacturonase; D002480:Cellulase