Reversed-phase separations of nitrogenous phospholipids on an octadecanoyl poly(vinyl alcohol) phase.
Molecular species of nitrogenous phospholipids (PLs) phosphatidylcholine (PC), phosphatidylethanolamine (PE), PE-derivatives and sphingomyelin (SP) were separated on an octadecanoyl poly(vinyl alcohol) (ODPVA) column by reversed-phase HPLC with UV and evaporative light scattering detection (ELSD). Mobile phases employed variable proportions of acetonitrile, methanol and water. HPLC-UV of the polar lipids yield components with peak intensities somewhat different from those obtained by HPLC-ELSD despite discernible similarity in the peak profiles observed in the two detection systems. Incorporation of ammonium hydroxide in mobile phases resulted in a decrease in analyte retention. The mobile phase basicity effect on capacity factors of PE species was significantly greater than that of PC counterparts. The new ODPVA-HPLC-ELSD technique was applied to the analysis of PC and PE molecular species in vegetable oils.
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National Center for Agricultural Utilization Research, US Department of Agriculture, Peoria, IL 61604, USA.
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D064753:Ammonium Hydroxide; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006878:Hydroxides; D015394:Molecular Structure; D009584:Nitrogen; D010713:Phosphatidylcholines / Q000737:chemistry / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification*; D011142:Polyvinyl Alcohol; D013109:Sphingomyelins / Q000737:chemistry / Q000302:isolation & purification
D006878:Hydroxides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D013109:Sphingomyelins; D064753:Ammonium Hydroxide; D011142:Polyvinyl Alcohol; D009584:Nitrogen