Oligomerization of the amide sensor protein AmiC by x-ray and neutron scattering and molecular modeling.
AmiC is the negative regulator of the amidase operon which is involved in amide metabolism in the cytosol of Pseudomonas aeruginosa. Crystal structures show that AmiC contains two large domains that are very similar to the periplasmic leucine-isoleucine-valine binding protein (LivJ) of Escherichia coli. Synchrotron X-ray and neutron (in 100% 2H2O buffer) scattering data were obtained for AmiC in the presence of its substrate acetamide and its anti-inducer butyramide which binds more weakly to AmiC than acetamide. Guinier analyses to obtain radius of gyration RG and molecular weight Mr values showed that AmiC formed trimers whose formation was favored in the presence of acetamide and which exhibited concentration-dependent properties at concentrations between 0.4 and 2 mg/mL. Above 2 mg/mL, where trimers predominated, the RG data were identical within 0.05 nm for AmiC-acetamide and AmiC-butyramide with mean X-ray and neutron RG values of 3.35 and 3. 28 nm, respectively. Scattering curve fits constrained by the crystal structure of AmiC-acetamide were evaluated in order to describe a model for trimeric AmiC. A translational search of parallel alignments of three monomers to form a symmetric AmiC homotrimer gave a good X-ray curve fit. Combinations of calculated curves for monomeric, dimeric, trimeric, and tetrameric AmiC as seen in the crystal structure of AmiC gave reasonable but weaker X-ray curve fits which did not favor the existence of tetrameric AmiC. It is concluded that AmiC exhibits novel ligand-dependent oligomerization properties in solution when these are compared to other members of the periplasmic binding protein superfamily, where AmiC exists in monomeric and trimeric forms, the proportions of which depend on the presence of acetamide or butyramide.
Chamberlain
D
D
Department of Biochemistry, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K.
O'Hara
B P
BP
Wilson
S A
SA
Pearl
L H
LH
Perkins
S J
SJ
D001426:Bacterial Proteins / Q000737:chemistry*; D003198:Computer Simulation; D008432:Mathematical Computing; D008958:Models, Molecular; D009502:Neutrons; D009842:Oligopeptides / Q000737:chemistry; D033902:Periplasmic Binding Proteins; D011485:Protein Binding; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa; D012097:Repressor Proteins / Q000737:chemistry*; D012542:Scattering, Radiation; D012996:Solutions; D014965:X-Rays
D001426:Bacterial Proteins; D009842:Oligopeptides; D033902:Periplasmic Binding Proteins; D012097:Repressor Proteins; D012996:Solutions; C070135:AmiC protein, Pseudomonas aeruginosa