Development of an immunoassay for larch arabinogalactan and its use in the detection of larch arabinogalactan in rat blood.
We describe a sensitive and convenient immunoassay for larch arabinogalactan and demonstrate its specificity for larch arabinogalactan. Anti-larch arabinogalactan antiserum is about 10(4) and 10(6) times more selective for detecting larch arabinogalactan than antiserum binds to branch terminal disaccharides consisting of the terminal beta-D-galactosyl residue and the penultimate branch (1-->6)-beta-D-galactosyl residue. It does not bind L-arabinose. The sensitivity of the assay for larch arabinogalactan is less than 0.1 microgram/mL. The application of the assay for measuring arabinogalactan pharmacokinetics in rat blood is illustrated.
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Advanced Magnetics, Inc., Cambridge, MA 02138, USA.
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D000818:Animals; D037263:Asialoglycoprotein Receptor; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002241:Carbohydrates / Q000494:pharmacology; D003429:Cross Reactions; D005685:Galactans / Q000032:analysis* / Q000097:blood* / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D011956:Receptors, Cell Surface; D012680:Sensitivity and Specificity; D014197:Trees
D037263:Asialoglycoprotein Receptor; D002241:Carbohydrates; D005685:Galactans; D006023:Glycoproteins; D007106:Immune Sera; D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides; D011956:Receptors, Cell Surface; C017707:Ricinus communis agglutinin-1; C005653:arabinogalactan