Induction of IP-10 chemokine promoter by measles virus: comparison with interferon-gamma shows the use of the same response element but with differential DNA-protein binding profiles.
Measles virus (MV) and interferon (IFN)-gamma induced IP-10 chemokine mRNA in U373 glioblastoma cells. The minimal response element for both MV and IFN-gamma was localized between nucleotide -231 and -153 of muIP-10 promoter, which contains an IFN-stimulated response element (ISRE) and the distal NF-kappa Bd site. Mutation of individual elements showed that ISRE and NF-kappa Bd were required to function together. DNA-protein binding profiles with the minimal response element showed that IFN-gamma induced a complex consisting of STAT1 while MV induced a complex consisting of p50 and p65 in the absence of new protein synthesis. IFN-gamma and MV also induced IRF-1 DNA binding activity which persisted for longer time periods with IFN-gamma stimulation. Despite the functional requirement of both ISRE and NF-kappa Bd elements, different combinations of DNA binding factors are used in the induction of IP-10 by MV or IFN-gamma.
Nazar
A S
AS
Department of Neurology, University of Maryland, Baltimore 21201, USA.
Cheng
G
G
Shin
H S
HS
Brothers
P N
PN
Dhib-Jalbut
S
S
Shin
M L
ML
Vanguri
P
P
D001483:Base Sequence; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D004279:DNA, Viral / Q000276:immunology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology; D017353:Gene Deletion; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D015967:Gene Expression Regulation, Viral / Q000276:immunology; D005909:Glioblastoma; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D008459:Measles virus / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000276:immunology; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D011401:Promoter Regions, Genetic / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000821:virology
D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D016207:Cytokines; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D016328:NF-kappa B; D012333:RNA, Messenger; D007371:Interferon-gamma