Electroporation of intact cells of Streptomyces parvulus and Streptomyces vinaceus.
Various assays of classical PEG-assisted transformation as well as electrotransformation of Streptomyces parvulus IMET41380 and Streptomyces vinaceus NCIB8852 are described. Contrary to the so far reported assays of electrotransforming Streptomyces strains, electroporation in S. parvulus and S. vinaceus was carried out on intact cells, without any lysozyme treatment. In these two strains, the classical PEG-assisted transformation of protoplasts does not work efficiently (10(3) to 10(4) transformants per micrograms of pIJ702 DNA) and electrotransformation gives 10 to 100 times higher yields (10(5) transformants per micrograms of pIJ702 DNA). The electroporation method described here is not applicable to other Streptomyces strains (S. lividans or S. coelicolor).
Mazy-Servais
C
C
Centre d'Ingénierie des Protéines, Université de Liège, Sart Tilman, Belgium.
Baczkowski
D
D
Dusart
J
J
D018274:Electroporation; D009113:Muramidase / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D013302:Streptomyces / Q000235:genetics*; D014169:Transformation, Bacterial
D011092:Polyethylene Glycols; D009113:Muramidase