title: Sodium-dependent proline and glutamate uptake by hippocampal synaptosomes during postnatal development.
abstract: NA(+)-dependent uptake of proline and glutamate by hippocampal synaptosomes was studied during postnatal development. At all ages from 9 days to adulthood, hippocampal synaptosomes transported proline by both a high-affinity and a low-affinity process, whereas glutamate was always transported predominantly by a high-affinity process. During the period of rapid synaptogenesis, the KT for high-affinity proline transport overshot the adult value, whereas the KT for glutamate transport increased steadily toward the adult value. The ratio of KT values for proline and glutamate was 2-3 times the adult value between 12 and 24 days of age. Although high-affinity transporters for proline and glutamate are expressed by nearly the same hippocampal pathways, they are differentially regulated during postnatal development.
authors.affiliation: Department of Pharmacology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Cohen
authors.forename: J V
authors.initials: JV
authors.lastname: Nadler
mesh_terms: D000818:Animals; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D017729:Presynaptic Terminals / Q000378:metabolism; D011392:Proline / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000502:physiology*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D018698:Glutamic Acid; D011392:Proline; D012964:Sodium