[Development of monoclonal antibody-based immunoenzyme kit for detection of human thyrotropin hormone].
An enzyme immunoassay kit based on monoclonal antibodies (Mab) has been developed for measuring thyroid-stimulating hormone in human serum and plasma. Laboratory trials demonstrated its high sensitivity (3.1 microIU/ml), specificity, reliability, and accuracy. The analysis is simple and rapid (2.5 h), which is convenient for clinical use. The kit permits differentiation between normal subjects and patients with primary hypo- or hyperthyroidism and helps monitor the treatment efficacy. Comparison with the Amerlite TSH-60 kit, carried out on 357 sera, showed a high correlation coefficient (0.96) between the two kits.
Sigal
E R
ER
Koriazova
L K
LK
Kovalevskaia
G I
GI
Pozdniakova
T A
TA
Kandyba
E I
EI
Kuznetsov
V A
VA
Vasilov
R G
RG
D000911:Antibodies, Monoclonal; D003937:Diagnosis, Differential; D006801:Humans; D006980:Hyperthyroidism / Q000175:diagnosis; D007037:Hypothyroidism / Q000175:diagnosis; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013972:Thyrotropin / Q000097:blood*
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic; D013972:Thyrotropin