title: Metabolism of estrogens and androgens by embryonic tissues of Arctic charr, Salvelinus alpinus.
abstract: This study examines the ability of Arctic charr (Salvelinus alpinus) embryos to metabolize tritiated androstenedione (A4), testosterone (T), 17 beta-estradiol (E2), and estrone (E1) in vitro; the metabolic products were separated by HPLC. A4 was poorly metabolized, with 48 to 64% of the substrate remaining even after 24 hr of incubation. The major metabolites of A4 metabolism are E1 and some other unidentified metabolites. T was mostly converted to A4, along with some reduced steroids, but E2 was a minor metabolite. Further, while E2 was almost exclusively transformed into E1, when E1 was used as the precursor, there was little metabolism; the products of E1 metabolism were small amounts of E1 sulfate, glucuronide, E2, and an unknown metabolite which cochromatographed with reference steroid androstenetrione (also called 11-ketoandrostenedione). It is concluded that in Arctic charr embryos there is preferential expression of a form of 17 beta-hydroxysteroid dehydrogenase resembling the type 2 isozyme of mammals that converts T and E2 to A4 and E1, respectively.
authors.affiliation: Department of Zoology, University of Guelph, Ontario, Canada.
authors.forename: M N
authors.initials: MN
authors.lastname: Khan
authors.forename: R L
authors.initials: RL
authors.lastname: Renaud
authors.forename: J F
authors.initials: JF
authors.lastname: Leatherland
mesh_terms: D000728:Androgens / Q000378:metabolism*; D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D014337:Trout / Q000196:embryology* / Q000378:metabolism
chemical_list: D000728:Androgens; D004967:Estrogens; D005965:Glucuronates; D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; D001141:Aromatase