Nuclease susceptibility of the rat liver satellite DNA-containing chromatin decreases with age.
Nuclease susceptibility of the satellite DNA-containing chromatin of the liver of young (18 +/- 2 weeks) and old (100 +/- 5 weeks) rats was analysed using nick-translated rat 185 bp satellite I DNA fragment cloned in pBR322. With increasing concentration of DNaseI and micrococcal nuclease (MNase), multimeric forms of the satellite ladder gradually disappear in both the ages. The rate of disappearance is faster in young rats as compared to old ones. Such age-dependent decrease in the susceptibility of satellite DNA-containing chromatin reflects its condensation towards heterochromatization in old age.
Thakur
M K
MK
Biochemistry and Molecular Biology Laboratory, Centre of Advanced Study in Zoology, Banaras Hindu University, Varanasi, India.
Chaurasia
P
P
D000367:Age Factors; D000818:Animals; D015139:Blotting, Southern; D002843:Chromatin / Q000187:drug effects* / Q000235:genetics; D004276:DNA, Satellite / Q000187:drug effects* / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry; D008297:Male; D008836:Micrococcal Nuclease / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D002843:Chromatin; D004276:DNA, Satellite; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease