Kainate-induced inhibition of voltage-activated potassium currents in cultured chick telencephalic neurons.
Kainate indirectly produces an inhibition of voltage-activated outward potassium currents on cultured chick telencephalic neurons, besides directly evoking activation of AMPA/kainate receptors (current with linear I-V curve, reversal potential near 0 mV). The former effect is also mediated by AMPA/kainate receptors. The role of [Ca2+]i in the inhibition was investigated by whole cell- and nystatin-perforated patch-clamp technique. Elevation of [Ca2+]i evoked by the Ca2+ ionophores ionomycin and A23187 caused an inhibition of outward currents as well. When the elevation of [Ca2+]i is prevented, the inhibition is decreased. These results suggest that [Ca2+]i elevation may be involved in the process.
Mike
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A
Institute of Experimental Medicine, Budapest, Hungary.
Dyatlov
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V
Vizi
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ES
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004553:Electric Conductivity; D007608:Kainic Acid / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D011188:Potassium / Q000037:antagonists & inhibitors* / Q000502:physiology*; D013687:Telencephalon / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*
D011188:Potassium; D007608:Kainic Acid