Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density-gradient centrifugation.
A method for isolation of enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of experimentally infected cats was developed using Percoll density-gradient centrifugation. Gamonts and merozoites were obtained essentially free of host-cell debris. A recovery rate of nearly 30% of the parasites in the original preparations was obtained by this method. Merozoites were separated from gamonts by filtration through a 3-micron polycarbonate filter.
Omata
Y
Y
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Taka
A
A
Terada
K
K
Koyama
T
T
Kanda
M
M
Saito
A
A
Dubey
J P
JP
D000818:Animals; D002371:Cat Diseases / Q000469:parasitology*; D002415:Cats; D002499:Centrifugation, Density Gradient; D005260:Female; D007413:Intestinal Mucosa / Q000469:parasitology*; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D014122:Toxoplasma / Q000254:growth & development / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000469:parasitology*
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone