Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium.
Thiamine pyrophosphate (TPP) is a required cofactor in Salmonella typhimurium that is generated de novo by the condensation of 4-amino-5-hydroxymethyl pyrimidine (HMP) pyrophosphate and 4-methyl-5-(beta-hydroxyethyl)-thiazole (THZ) monophosphate. The THZ and HMP moieties are independently synthesized, and labeling studies have demonstrated probable metabolic precursors to both. We present herein the initial characterization of thiI, a gene required for THZ synthesis. We show that thiI is a 1,449-bp open reading frame located at minute 9.65 on the S. typhimurium chromosome and that it encodes a 483-amino-acid protein with a predicted molecular mass of 55 kDa. Unlike genes in the thiamine biosynthetic operon at minute 90, thiI is not transcriptionally regulated by TPP.
Webb
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Department of Bacteriology, University of Wisconsin-Madison, 53706, USA.
Claas
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Downs
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DM
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013466:Sulfurtransferases; D013844:Thiazoles / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D013844:Thiazoles; C012572:2-(4-methyl-1,3-thiazol-5-yl)ethanol; D013466:Sulfurtransferases; C107110:ThiI protein, bacteria