title: Solid-phase synthesis and cellular localization of a C- and/or N-terminal labelled peptide.
abstract: We report the solid-phase synthesis by the Fmoc strategy of a peptide containing a cysteamide group at its C-terminus. This peptide was subjected to further modifications including the linkage of fluorophores, namely lucifer yellow and coumarin respectively, at the C- and/or N-terminals. After incubation with living cultured cells these two probes were localized and it is concluded that the post-synthesis modifications can strongly modify the localization of the peptide.
authors.affiliation: Centre de Recherche de Biochimie Macromoléculaire, Montpellier, France.
authors.forename: P
authors.initials: P
authors.lastname: Vidal
authors.forename: L
authors.initials: L
authors.lastname: Chaloin
authors.forename: J
authors.initials: J
authors.lastname: Méry
authors.forename: N
authors.initials: N
authors.lastname: Lamb
authors.forename: N
authors.initials: N
authors.lastname: Lautredou
authors.forename: R
authors.initials: R
authors.lastname: Bennes
authors.forename: F
authors.initials: F
authors.lastname: Heitz
mesh_terms: D000595:Amino Acid Sequence; D005453:Fluorescence; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis* / Q000138:chemical synthesis*; D013057:Spectrum Analysis
chemical_list: D010455:Peptides