[The induction of mutations in Streptomyces aureofaciens by UV rays and nitrosoguanidine and the identification of the mutants obtained].
Streptomyces aureofaciens 019(8), chlortetracycline producent was subjected to the effect of UV-light and nitrosoguanidine and after that its 121520 colonies have been analyzed by the methods of replicas and 57 mutants were isolated: sensitive to the effect of UV radiation--14; auxotrophs--30; antibiotic-inactive --13. The induction frequency of mutations is within the limits of 0.016 to 0.067%. Auxotrophic mutants manifested genetic instability. Mutants with a block chlortetracyclin biosynthesis are stable.
Lavrinchuk
V Ia
VIa
Stryzhkova
H M
HM
Matseliukh
B P
BP
D002751:Chlortetracycline / Q000096:biosynthesis; D003470:Culture Media; D016296:Mutagenesis / Q000187:drug effects* / Q000528:radiation effects*; D009154:Mutation / Q000235:genetics*; D009604:Nitrosoguanidines / Q000494:pharmacology*; D010641:Phenotype; D013304:Streptomyces aureofaciens / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D014466:Ultraviolet Rays
D003470:Culture Media; D009604:Nitrosoguanidines; D002751:Chlortetracycline