title: High levels of viremia in hu-PBL-NOD-scid mice with HIV-1 infection.
abstract: We studied the compatibility of human lymphocyte engraftment and susceptibility to HIV-1 infection in 2 new immunodeficient mice. NOD/Shi-scid mice were generated by backcrossing of the scid mutation into NOD mice while C57BL/6-RAG2(0/0) were generated by knocking out the RAG-2 gene. Human T lymphocytes were reconstituted in new immunodeficient mouse strains. We found that the new immunodeficient mouse strains accepted human PBL engraftment and HIV-1 infection more efficiently than conventional C.B-17-scid mice. Especially in the hu-PBL-NOD/Shi-scid strain, we reproduced the high levels of HIV-1 viremia comparable to or at significantly higher levels than after HIV-1 primary infection. These results indicate that our hu-PBL-NOD-scid animal is useful for investigations of the activation mechanism in HIV-1 replication in vivo and after primary infection.
authors.affiliation: Department of Molecular Virology, Tokyo Medical and Dental University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Koyanagi
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: R
authors.initials: R
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Misawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kawano
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Miyasaka
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Ueyama
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000503:physiopathology* / Q000821:virology; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000637:transplantation / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000637:transplantation / Q000821:virology; D003433:Crosses, Genetic; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D004198:Disease Susceptibility; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D017710:Lymphocyte Transfusion; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D009687:Nuclear Proteins; D013045:Species Specificity; D014183:Transplantation, Heterologous; D014766:Viremia / Q000503:physiopathology*
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C499311:RAG2 protein, human; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2