title: Inhibition of phospholipase C gamma 1 by the prenylated flavonoids from Sophora flavescens.
abstract: The effect on the phospholipase C gamma 1 activity of eleven prenylated flavonoids from Sophora flavescens was investigated. These flavonoids exhibited relatively strong inhibitory activity with IC50 values ranged from 7.5 x 10(-6) M to 35 x 10(-5) M with the exception of kushenol H (4) (IC50 value; > 5.3 x 10(-4) M). The presence of C3-OH resulted in a significant diminution of activity and the configuration of C3-OH is likely to be another factor influencing the activity. In addition, hydration of the C-4"'-C-5"' double bond of the lavandulyl side chain caused complete loss of activity. These data suggest that the presence and configuration of C3-OH are related to the inhibitory activity and the lavandulyl side chain is also important for high inhibitory activity against PLC gamma 1.
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: H R
authors.initials: HR
authors.lastname: Ko
authors.forename: S Y
authors.initials: SY
authors.lastname: Ryu
authors.forename: W K
authors.initials: WK
authors.lastname: Oh
authors.forename: B Y
authors.initials: BY
authors.lastname: Kim
authors.forename: S C
authors.initials: SC
authors.lastname: Ahn
authors.forename: T I
authors.initials: TI
authors.lastname: Mheen
authors.forename: J S
authors.initials: JS
authors.lastname: Ahn
mesh_terms: D000818:Animals; D002417:Cattle; D002531:Cerebellum / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D015394:Molecular Structure; D051966:Phospholipase C gamma; D013329:Structure-Activity Relationship; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D005419:Flavonoids; D007527:Isoenzymes; D010738:Type C Phospholipases; D051966:Phospholipase C gamma