Detection of single-molecule interactions using correlated thermal diffusion.
Observation of discrete, single-molecule binding events allows one to bypass assumptions required to infer single-molecule properties from studies of ensembles of molecules. Optically trapped beads and glass microneedles have been applied to detect single-molecule binding events, but it remains difficult to identify signs of binding events given the large displacements induced by thermal forces. Here, we exploit thermal diffusion by using correlation between motion of optically trapped beads attached to both ends of a single actin filament to track binding events of individual myosin molecules. We use correlated diffusion to measure the stiffness of a single myosin molecule and estimate its thermal fluctuation in a poststroke state as comparable in amplitude to the measured stroke distance. The use of correlated diffusion to measure kinetics of single-molecule interactions and the stiffness of the interacting moieties should be applicable to any pair of interacting molecules, and not limited to biological motors.
Mehta
A D
AD
Departments of Biochemistry and Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, CA 94305, USA.
Finer
J T
JT
Spudich
J A
JA
D000199:Actins / Q000737:chemistry*; D004058:Diffusion; D006358:Hot Temperature; D009218:Myosins / Q000737:chemistry; D011485:Protein Binding
D000199:Actins; D009218:Myosins