Effects of brefeldin A on the expression and transport of influenza A virus haemagglutinin, M1 and M2 proteins within the cell.
Brefeldin A (BFA) decreased the expression of influenza A virus haemagglutinin (HA) and M2 protein on the plasma membrane of virus-infected MDCK cells. It caused a retention of M1 protein in the cell nucleus and a decrease of its expression on the plasma membrane. On the other hand, an increased labelling of the cytoplasmic domain of M2 protein on the plasma membrane in BFA-treated cells was observed in contrast to the labelling in BFA-untreated cells. The effects of BFA on the microtubules and cellular motors are discussed.
Ciampor
F
F
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Závodská
E
E
Cmarko
D
D
Cmarková
J
J
Varecková
E
E
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D020126:Brefeldin A; D002451:Cell Compartmentation / Q000187:drug effects; D002460:Cell Line; D003517:Cyclopentanes / Q000494:pharmacology*; D004285:Dogs; D006389:Hemagglutinins, Viral / Q000378:metabolism*; D007150:Immunohistochemistry; D007473:Ion Channels / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000378:metabolism*; D000086082:Viroporin Proteins
D000954:Antigens, Surface; D020126:Brefeldin A; D003517:Cyclopentanes; D006389:Hemagglutinins, Viral; D007473:Ion Channels; D008565:Membrane Proteins; D014763:Viral Matrix Proteins; C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins