Isocratic high-performance liquid chromatographic method for the separation of isradipine and its main metabolites. Application to in vitro metabolization by h3A4/OR cells.
A reversed-phase HPLC method was developed for the study of isradipine oxidation in vitro. The drug and its main metabolites were determined after extraction from culture media and from h3A4/OR cells having the cytochrome P450 isoenzyme involved in the xenobiotic metabolization. The HPLC assay was fully validated and was used to follow the biotransformation kinetics of isradipine.
Bidouil
S
S
Université Libre de Bruxelles, Institut de Pharmacie, Laboratoire de Chimie Bioanalytique et Toxicologie, Brussels, Belgium.
Dubois
J
J
Hanocq
M
M
D002121:Calcium Channel Blockers / Q000302:isolation & purification* / Q000378:metabolism; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D017275:Isradipine / Q000302:isolation & purification* / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002121:Calcium Channel Blockers; D017275:Isradipine