Macrophage modifying factor secreted by the tetrathyridia of Mesocestoides corti (Cestoda): monoclonal antibody to the modifying factor antagonizes its immunological activity.
Immunomodulation of macrophage activity by in vitro secretions of Mesocestoides corti has been previously demonstrated. The modifying activity secreted by M. corti had the effect of reducing the normal accessory function of macrophages in a Con-A-activated lymphocyte proliferation assay. This paper describes the purification of the modifying activity by FPLC techniques and the generation of a monoclonal antibody (MoAb) to this molecule in mice. The MoAb bound immunomodulatory FPLC fractions of M. corti in an ELISA. When MoAb was applied in conjunction with immunomodulatory parasite secretions to macrophages in vivo or in vitro, the modifying effect of the secretions was abolished. This profound effect of the MoAb should help to elucidate the mechanisms by which metacestode parasites avoid host immune responses and may enable therapeutic intervention.
Kadian
S K
SK
Department of Veterinary Pathology, University of Liverpool, UK.
Dixon
J B
JB
Carter
S D
SD
Jenkins
P
P
D000276:Adjuvants, Immunologic / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000818:Animals; D000909:Antibodies, Helminth / Q000494:pharmacology; D000947:Antigens, Helminth / Q000302:isolation & purification; D001681:Biological Assay; D002590:Cestode Infections / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008645:Mesocestoides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth