Effects of 17 beta estradiol on the metabolism of labelled arachidonic acid in uteri isolated from intact and ovariectomized rats. Influence of a restricted diet.
The effects of restricted diet (50% of the normal intake during 25 days) on the metabolism of 14U arachidonic acid, were explored in uterine horn strips isolated from intact and ovariectomized rats, treated by 17 beta-estradiol or controls. The metabolism of arachidonic acid into different eicosanoids, PGE2, PGF2alpha, 6-keto PGF1alpha and TXB2, showed that the restricted diet diminished PGE2 and PGF2alpha, in intact rats, significantly. In contrast, this kind of feeding did not produce any change in castrated rats. Tissue preparations from previously estrogenized intact and castrated normal-fed rats showed that the production of different metabolites decreased. A similar result was obtained in intact rats subjected to a restricted diet. Nevertheless, in castrated underfed rats, estrogens did not produce any effect on the various eicosanoids analysed. These results showed that in isolated uteri, the effects of 17 beta-estradiol, on metabolite production from labelled arachidonic acid, are different from controls in ovariectomized diet-restricted rats.
Casalino-Matsuda
S M
SM
Cátedra de Fisiología, Facultad de Odontología, Universidad Nacionalde Córdoba, Argentina.
Linares
J A
JA
Goldraij
A
A
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D004032:Diet; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005508:Food Deprivation; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013929:Thromboxane B2 / Q000096:biosynthesis; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism*
D016718:Arachidonic Acid; D004958:Estradiol; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D015232:Dinoprostone