Establishment and characteristics of a practical and useful astrocyte cell line transformed by a temperature-sensitive mutant of simian virus 40.
A practical mouse astrocyte cell line (A640-IG) was established by transformation with a temperature-sensitive mutant of simian virus 40 (SV40) and the relationship between the function of SV40 large T antigen and the growth and differentiation of A640-IG cells, which are most clearly dependent on temperature that ever established, was reported. A640-IG cells proliferated actively with expression of large T antigen when they were cultured at 33 degrees C. They had a fibroblast-like appearance, and displayed faint immunoreactivity with an antibody against glial fibrillary acidic protein (GFAP). However, when large T antigen expression ceased at 39 degrees C, the cells did not grow actively and differentiated into astrocytes as demonstrated by both their morphological and immunohistochemical characteristics. Differentiation into astrocytes was more obvious when the cells were plated on bacteriological dishes in high density. Western blotting confirmed immunohistochemical observations. A640-IG cells thus showed contrasting behaviour in terms of cell growth and differentiation depending on the temperature. This unique and practical astrocyte cell line is a useful model for investigating the mechanisms of astrocyte growth and differentiation.
Kitamura
Y
Y
Second Department of Pathology, Faculty of Medicine, Tottori University, Yonago, Japan.
Michikawa
M
M
Tanigawa
T
T
Katsumoto
T
T
Morita
T
T
Nanba
E
E
Ohama
E
E
Terada
T
T
D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D051379:Mice; D008813:Mice, Inbred ICR; D013539:Simian virus 40 / Q000502:physiology*; D013696:Temperature