Promoter sequences from two different Brassica napus tapetal oleosin-like genes direct tapetal expression of beta-glucuronidase in transgenic Brassica plants.
To investigate the sequences responsible for the regulated expression of tapetal-specific oleosin-like genes, ca. 2 kb of the 5'-upstream regions from two divergent genes, OlnB;4 and OlnB;13, were isolated, sequenced and fused to the reporter gene beta-glucuronidase for study in transgenic Brassica napus plants. Although the proteins encoded by these two genes are highly divergent, except for the conserved oleosin-like domain, the first 250 bp of their 5'-upstream regions was 86% identical, including a region of 150 bp upstream from the TATA box. Analysis of 42 independent transformants by histochemical and fluorometric methods showed that both promoters directed tapetal-specific expression that peaked at the 4 mm flower bud stage.
Hong
H P
HP
Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, Canada.
Ross
J H
JH
Gerster
J L
JL
Rigas
S
S
Datla
R S
RS
Hatzopoulos
P
P
Scoles
G
G
Keller
W
W
Murphy
D J
DJ
Robert
L S
LS
D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D014644:Genetic Variation; D005966:Glucuronidase / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D011994:Recombinant Proteins; D005966:Glucuronidase