A simple novel method for studying the combined inhibitory effects of ethylurea and N,N-dimethylurea on jack bean urease.
The kinetics of the inhibition of jack bean urease in the presence of ethylurea and N,N-dimethylurea was studied at pH = 7.0. Both inhibitors were competitive inhibitors. A simple novel method was used for determining the dissociation constants (Ki) values for the inhibition which were 26 and 28 mM respectively.
Saboury
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Department of Chemistry, University of Tarbiat-Modares, Tehran, Iran.
Moosavi-Movahedi
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D001667:Binding, Competitive / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007887:Fabaceae / Q000201:enzymology*; D007700:Kinetics; D008782:Methylurea Compounds / Q000494:pharmacology*; D010946:Plants, Medicinal; D014508:Urea / Q000031:analogs & derivatives* / Q000494:pharmacology; D014510:Urease / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D008782:Methylurea Compounds; C007685:ethylurea; D014508:Urea; D014510:Urease