title: Changes of neutrophil migration without modification of in vitro metabolism and adhesion in Behçet's disease.
abstract: OBJECTIVE
  Increase of neutrophil chemotaxis in Behçet's disease (BD) has been described, but it is not clear whether there is a correlation with other variables of neutrophil function and whether these modifications correlate with disease activity.
  
  
  METHODS
  We studied neutrophil functions in patients with BD in the acute phase in comparison with healthy control subjects and with the same patients during disease remission, with or without therapy. We investigated in vivo neutrophil migration by Senn's skin window technique and measured adhesion assay and superoxide production in circulating and migrating neutrophils after different stimuli.
  
  
  RESULTS
  Neutrophil migration in vivo was 101.3 +/- 17.9 x 10(6) polymorphonuclear lymphocytes (PMN)/cm2/24 h in patients with BD in the acute phase and 66.1 +/- 7.8 x 10(6) PMN/cm2/24 h in controls (p < 0.001). No correlation was found between leukocyte counts and neutrophil migration. Neutrophil migration evaluated in the same patients in a phase of disease remission was 58.3 +/- 10.3 x 10(6) PMN/cm2/24 h (p < 0.001 vs acute phase, not significant vs controls). The neutrophils of the exudate were normally primed to response to the chemotactic peptide fMLP. No differences between the 2 groups were found in superoxide production, adhesion under basal conditions, or in response to different stimuli by circulating and migrating neutrophils.
  
  
  CONCLUSIONS
  Abnormally high migration of neutrophils in the active phase of BD is the only consistent neutrophil dysfunction. Since this modification is reversed by therapy, the evaluation of in vivo neutrophil migration may be useful in diagnosing and monitoring disease activity. Blood neutrophils have normal responses to different stimuli, indicating they are not primed by the disease state.
authors.affiliation: Institute of Patologia Medica, University of Verona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Carletto
authors.forename: M L
authors.initials: ML
authors.lastname: Pacor
authors.forename: D
authors.initials: D
authors.lastname: Biasi
authors.forename: P
authors.initials: P
authors.lastname: Caramaschi
authors.forename: S
authors.initials: S
authors.lastname: Zeminian
authors.forename: P
authors.initials: P
authors.lastname: Bellavite
authors.forename: L M
authors.initials: LM
authors.lastname: Bambara
mesh_terms: D000328:Adult; D001528:Behcet Syndrome / Q000175:diagnosis / Q000276:immunology*; D002273:Carcinogens / Q000494:pharmacology; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects / Q000276:immunology*; D009504:Neutrophils / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D012867:Skin / Q000276:immunology / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000276:immunology / Q000494:pharmacology
chemical_list: D002273:Carcinogens; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate