Retinoic acid-induced alteration of cell surface topography and other changes in an oral carcinoma cell line in culture.
Nature of antiproliferative action of retinoic acid (RA) on KB cells was studied by using monolayer and agar culture techniques. RA-treated cultures showed increased requirement of serum for their growth. Growth of colonies in agar culture was significantly retarded when cells were treated with 40 mumol RA. RA-induced growth inhibitions in both monolayer and agar cultures were independent of cell seeding densities. Cortisone and hydrocortisone showed no reversal of the inhibitory effects induced by RA on KB cells. Scanning electron microscopy study revealed a significant alteration in cell surface topography of RA-treated cells in monolayer culture. The results demonstrate that RA has a potential of reversing some of the properties which are associated with transformed state of oral carcinoma cells.
Sarkar
R
R
Chittaranjan National Cancer Institute, Calcutta, India.
Das
S K
SK
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D003348:Cortisone / Q000494:pharmacology; D004347:Drug Interactions; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007624:KB Cells / Q000187:drug effects / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D014212:Tretinoin / Q000494:pharmacology*
D000970:Antineoplastic Agents; D014212:Tretinoin; D003348:Cortisone; D006854:Hydrocortisone