[High homology between antigen of HFRSV with anti-idiotype antibody against HFRSY].
OBJECTIVE
To detect the structural characterization of genes of the anti-idiotype antibody and the antigen.


METHODS
The hybridoma (C8) secreted the anti-idiotype human McAb against hemorrhagic fever virus (HFRSV). The variable region genes of heavy and light chain of the anti-Id huMcAb were cloned. The homology was analysed between the variable region genes and the genes of HFRSV.


RESULTS
The 45-55 amino acids of VH and 58-68 amino acids of VL were highly homologic to the 447-457 amino acids of G2 protein in Hantaan virus. And the homologeneous regions were similar in secondary structure of the three proteins.


CONCLUSIONS
There is a molecular basis in the anti-idiotype antibody imaging antigen.
Gao
L
L
Department of Biochemistry, Fourth Militray Medical University, Xian.
Chen
S
S
Chen
N
N
D000888:Antibodies, Anti-Idiotypic / Q000235:genetics*; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000235:genetics*; D000939:Epitopes; D005814:Genes, Viral; D018063:Hantaan virus / Q000276:immunology*; D006801:Humans; D017386:Sequence Homology, Amino Acid
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes