Complement coating of erythrocytes is reduced following their interaction with neutrophils in vitro without loss of complement receptor 1 (CR1).
We have investigated the interaction of complement-coated erythrocytes (E) with neutrophils, in vitro, to determine the effects of erythrocyte CR1. Complement-coating in vitro, to mimic immune complex uptake, caused a reduction in E-CR1, but subsequent interaction with neutrophils effected a removal of E-C3b and a return of E-CR1 to levels approximating that of uncoated erythrocytes. These data indicate that C3b, associated with immune complexes, may bind to E-CR1 in a reversible manner and that subsequent interaction with phagocytes need not necessarily result in cleavage (and loss of) E-CR1. Further, we conclude that E-CRI can apparently be reduced by epitope masking.
Imrie
H J
HJ
Department of Immunology, Queen's Medical Centre, Nottingham, UK.
Jones
D R
DR
D000936:Antigen-Antibody Complex / Q000276:immunology; D003179:Complement C3b / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D017463:Receptors, Complement 3b / Q000276:immunology / Q000378:metabolism*
D000936:Antigen-Antibody Complex; D000939:Epitopes; D017463:Receptors, Complement 3b; D003179:Complement C3b