Structural characterization of the O-antigenic polysaccharide of Escherichia coli serotype 017 lipopolysaccharide.
The structure of the O-polysaccharide component of the lipopolysaccharide produced by Escherichia coli 017 (ATCC 23512) was determined by the use of methylation, periodate oxidation, one- and two-dimensional nuclear magnetic resonance spectroscopy, and mass spectrometric methods. The O-polysaccharide was found to be a high molecular weight polymer of repeating branched pentasaccharide units composed of D-mannose, D-glucose, and 2-acetamido-2-deoxy-D-glucose residues (3:1:1) and had the structure (formula: see text).
Masoud
H
H
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada.
Perry
M B
MB
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D004926:Escherichia coli / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*
D019081:O Antigens; C106472:O-antigen, E. coli O17