title: Neuroligand-triggered calcium signalling in cultured human glioma cells.
abstract: Cells from primary cultures of four glioblastomas (GB), three low-grade astrocytomas (A), and four low-grade oligodendrogliomas (O) were tested for the presence of neuroligand receptors linked to Ca2+ signalling by calcium imaging. Cells of days 3 to 21 in culture were incubated with 5 microM fluo-3-acetomethylester in a bath solution and stimulated with 0.1 mM ATP, 0.01 mM angiotensin II, bradykinin, histamine, norepinephrine, serotonin, and substance P for 15 s, with 0.01 mM glutamate and 50 mM K+ for 30 s. Changes in the Ca2+ concentration were measured with a confocal laser scanning microscope. In all glioma subtypes, the majority of cells showed Ca2+ responses after application of histamine (60% of cells tested in GB, 67% in A, 86% in O), bradykinin (66% in GB, 29% in A, 55% in O) and ATP (48% in GB, 70% in A, 47% in O). The other stimuli induced Ca2+ transients in a smaller proportion (between 33% and 2%) of the cells. Our study demonstrates that histamine, bradykinin and ATP are potent inducers of [Ca2+]i signals in gliomas.
authors.affiliation: Department of Cellular Neurosciences, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Weydt
authors.forename: T
authors.initials: T
authors.lastname: Möller
authors.forename: C
authors.initials: C
authors.lastname: Labrakakis
authors.forename: S
authors.initials: S
authors.lastname: Patt
authors.forename: H
authors.initials: H
authors.lastname: Kettenmann
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D005456:Fluorescent Dyes; D005909:Glioblastoma; D005947:Glucose / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D009837:Oligodendroglioma; D011188:Potassium / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology*; D013373:Substance P / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D014662:Vasoconstrictor Agents / Q000494:pharmacology
chemical_list: D000316:Adrenergic alpha-Agonists; D005456:Fluorescent Dyes; D018377:Neurotransmitter Agents; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D012701:Serotonin; D013373:Substance P; D006632:Histamine; D000255:Adenosine Triphosphate; D005947:Glucose; D011188:Potassium; D001920:Bradykinin; D002118:Calcium; D009638:Norepinephrine