title: Use of zonal centrifugation method for the preparation of mu-opioid receptor enriched membranes from bovine corpus striatum.
abstract: Earlier attempts to purify the opioid receptors met with limited success because of the use of brain crude membranes. Subcellular fractionation procedures adopted now to get enriched membranes resulted in 2-3 fold enrichment. However, a procedure has been developed in our laboratory in which a crude membrane fraction obtained at 17,500 x g was lysed with 1 mM sodium bicarbonate and later subjected to zonal fractionation, employing a density gradient of 0.6-1.2 M sucrose. This could yield a membrane fraction highly enriched with mu-opioid receptors which is 9.3 fold higher than the crude membranes.
authors.affiliation: Department of Neurochemistry, National Institute of Nutrition, Jamai Osmania, Hyderabad, India. icmrnin@ren.nic.in
authors.forename: K V
authors.initials: KV
authors.lastname: Sastry
authors.forename: K G
authors.initials: KG
authors.lastname: Harikumar
authors.forename: P
authors.initials: P
authors.lastname: Sailaja
authors.forename: M K
authors.initials: MK
authors.lastname: Janardana Sarma
mesh_terms: D000818:Animals; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure*; D002501:Centrifugation, Zonal / Q000379:methods; D003342:Corpus Striatum / Q000378:metabolism / Q000648:ultrastructure*; D004174:Diprenorphine / Q000378:metabolism; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D004745:Enkephalins / Q000378:metabolism; D011869:Radioligand Assay; D017450:Receptors, Opioid, mu / Q000819:agonists / Q000302:isolation & purification* / Q000378:metabolism; D013347:Subcellular Fractions / Q000648:ultrastructure; D013395:Sucrose; D013574:Synaptosomes / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D004745:Enkephalins; D017450:Receptors, Opioid, mu; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D004174:Diprenorphine; D013395:Sucrose