Characteristics of a cell line established from a case of acute megakaryoblastic leukemia.
Acute megakaryoblastic leukemia is uncommon and comprises about 5% of acute nonlymphoid leukemias in the French-American-British classification. Cell lines from such leukemias are relatively rare with only about 8 reported in the literature. We established a cell line from a case of acute megakaryoblastic leukemia arising in a 2-year-old child. Surface marker studies of the cells confirmed their megakaryoblastic nature, with 54% of the cells being CD61 positive and peroxidase and esterase negative. The cells had a doubling time of 72 h. Emperipolesis (a phenomenon in which a cell, usually a lymphocyte or neutrophil, enters another cell, moves about and leaves without undergoing phagocytosis) of one blast into another, larger one, was occasionally seen, and a review of the original bone marrow specimen also showed emperipolesis of neutrophils into the megakaryoblasts. The cells responded to interleukin 3 and were inhibited with all-trans-retinoic acid. The karyotype of the cells was the 46,XX,-16 with a marker chromosome. The marker chromosome is possibly chromosome 16 with a small segment of a chromosome translocated to the terminal portion of chromosome 16.
Skinnider
L F
LF
Department of Pathology, University of Saskatchewan, Saskatoon, Canada.
Pabello
P
P
Zheng
C Y
CY
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D018929:Cell Culture Techniques; D002455:Cell Division; D002467:Cell Nucleus / Q000473:pathology; D002675:Child, Preschool; D003593:Cytoplasm / Q000473:pathology; D006801:Humans; D039661:Integrin beta3; D007377:Interleukin-3 / Q000494:pharmacology; D007621:Karyotyping; D007947:Leukemia, Megakaryoblastic, Acute / Q000276:immunology / Q000473:pathology*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D011003:Ploidies; D063268:Sialic Acid Binding Ig-like Lectin 3; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D039661:Integrin beta3; D007377:Interleukin-3; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins; D063268:Sialic Acid Binding Ig-like Lectin 3; D014212:Tretinoin