[Effects of recombinant human tumor necrosis factor on the development of ascitic tumor and expression of c-erbB2 in the nude mouse models of ovarian cancer].
OBJECTIVE
To investigate the effects of recombinant human tumor necrosis factor (rhTNF) on the growth of ascitic tumor and expression of c-erbB2 in the nude mouse models of ovarian cancer.


METHODS
By intraabdominal injection, rhTNF was delivered to the peritoneal cavity of nude mouse with ovarian cancer as well as control group, after 6 days treatment, tumor cell counting and expression of c-erbB2 were detected by indirect fluorescence flow cytometry.


RESULTS
There is a significant difference in tumor cell counting between the study group and control group (P < 0.01). Expression of c-erbB2 in study group was lower than that in control group.


CONCLUSIONS
rhTNF was demonstrated to have effects on both inhibition of ascitis tumor development and down regulation of c-erbB2 expression in nude mouse with ovarian cancer.
Yin
D
D
Obstetric and Gynecology Hospital, Shanghai Medical University.
Zhang
X
X
Feng
Y
Y
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology; D018283:Cystadenocarcinoma, Papillary / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D018734:Genes, erbB-2; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D018719:Erb-b2 Receptor Tyrosine Kinases; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha