Solution structure of neuropeptide tyrosine 13-36, a Y2 receptor agonist, as determined by NMR.
The three-dimensional structure of neuropeptide tyrosine (NPY) 13-36, a specific Y2 receptor agonist, has been investigated by two-dimensional 1H-NMR spectroscopy in solution. Analysis of the double-quantum-filtered correlation spectroscopy (DQFCOSY), total correlation spectroscopy (TOCSY) and nuclear Overhauser enhancement spectroscopy (NOESY) spectra provided a complete assignment of the proton signals. The interproton connectivities observed in the NOESY spectra comprised 166 intraresidue and 95 interresidue distance ranges which were used as constraints for molecular modeling by distance geometry, simulated annealing and energy minimization. The optimal structures are characterized by a helical C-terminal fragment Leu30-Tyr36 and a wide loop from Leu17 to Ser22. The structure of NPY 13-36 is analogous to the structure of NPY under the same solvent conditions. Comparison with other reported Y2 agonists suggests that the helical Leu30-Tyr36 fragment is the most critical for activity.
Labelle
M
M
INRS-Santé, Université du Québec, Canada.
St-Pierre
S
S
Savard
R
R
Boulanger
Y
Y
D000818:Animals; D004121:Dimethyl Sulfoxide; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009478:Neuropeptide Y / Q000737:chemistry* / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*; D012996:Solutions; D013552:Swine; D013696:Temperature
D009478:Neuropeptide Y; D010446:Peptide Fragments; D017476:Receptors, Neuropeptide Y; D012996:Solutions; C062924:neuropeptide Y (13-36); C095889:neuropeptide Y2 receptor; D004121:Dimethyl Sulfoxide