Crystallization and preliminary crystallographic analysis of the N-terminal actin binding domain of human fimbrin.
We have crystallized the N-terminal actin binding domain (ABD1) of human fimbrin, a representative member of the largest class of actin crosslinking proteins. Diffraction from these crystals is consistent with the orthorhombic space group P2(1)2(1)2(1) (a = 50.03 A, b = 61.24 A, c = 102.30 A). These crystals contain one molecule in the asymmetric unit and diffract to at least 1.9 A resolution. The crystal structure of ABD1 will be the first structure of an actin crosslinking domain.
Goldsmith
S C
SC
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Pokala
N
N
Matsudaira
P
P
Almo
S C
SC
D002352:Carrier Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008840:Microfilament Proteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; C026742:plastin