Nucleic acid in situ probing. Hybridization to human chromosomes of an alkaline phosphatase labeled centromeric probe.
The main principles that underly the use of nucleic acid probes for in situ hybridization are summarized. These include probe design, target preparation, hybridization formats and conditions, and signal generating systems. These principles underly the specific protocol that is described, namely the use of an akaline phosphatase-labeled cloned sequence of the alphoid repeated DNA family as a centomere probe for human chromosomes.
Nardone
R M
RM
Center for Advanced Training in Cell Molecular Biology, Catholic University of America, Washington, DC 20064, USA.
D000469:Alkaline Phosphatase / Q000737:chemistry; D002503:Centromere; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D015341:Nucleic Acid Probes
D015341:Nucleic Acid Probes; D000469:Alkaline Phosphatase