title: Chromosomal homeologies between human, harbor seal (Phoca vitulina) and the putative ancestral carnivore karyotype revealed by Zoo-FISH.
abstract: We report on the construction of the first comparative Zoo-FISH map of a marine mammal. Zoo-FISH with DNA probes from a human chromosome-specific library to metaphase spreads of the harbor seal (Phoca vitulina) disclosed 31 conserved syntenic segments covering the complete autosomal complement and the X chromosome. Comparison with Zoo-FISH maps of other species reveals that the harbor seal shares a high degree of karyotypic homeology with the human complement and an even higher degree with the conordinal cat complement. These findings suggest that pinniped, felid and human karyotypes have maintained conserved complements. Based on data of Zoo-FISH and comparative cytogenetics, a Zoo-FISH map of the ancestral carnivore karyotype (Z-CAR) is proposed. Flow cytometry revealed that the DNA value of the harbor seal genome is 79% that of the human genome.
authors.affiliation: Abteilung für Humanbiologie und Humangenetik, Universität Kaiserslautern, Postfach 3049, D-67653 Kaiserslautern, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Frönicke
authors.forename: J
authors.initials: J
authors.lastname: Müller-Navia
authors.forename: K
authors.initials: K
authors.lastname: Romanakis
authors.forename: H
authors.initials: H
authors.lastname: Scherthan
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002335:Carnivora / Q000235:genetics*; D002415:Cats; D002875:Chromosomes / Q000235:genetics* / Q000648:ultrastructure*; D015342:DNA Probes; D015698:Genomic Library; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D046048:Seals, Earless / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015342:DNA Probes