title: Three-dimensional structure and position of porcine motilin in sodium dodecyl sulfate micelles determined by 1H NMR.
abstract: The solution structure of the porcine gastrointestinal peptide hormone motilin was determined in the presence of sodium dodecyl sulfate (SDS) micelles at 28 degrees C using 1H nuclear magnetic resonance, full relaxation matrix analysis, and structure calculations based on restrained molecular dynamics. The structure of motilin in SDS micelles is described by a reverse gamma-turn and a beta-turn of type II in the N terminal end, an alpha-helical region in the middle of the molecule, and an extended structure at the C terminus. The position of the motilin molecule relative to the SDS micelles was probed by adding spin-labeled stearic acids, containing 12-doxyl or 5-doxyl spin-labels. We observed selective broadening of the proton resonances of residues 3-5 and concluded that they must be located in the interior of the micelle. These experiments suggest a structural model in which the hydrophobic N terminus consists of two well-defined turns buried in the interior of the micelle, whereas the amphiphilic alpha-helical part is located at the surface of the micelle. Spectral density mapping using a 13C label on the alphaC of Leu10 gave overall rotational correlation times taum of 6.6 and 4.5 ns at 35 and 45 degrees C, respectively. The long correlation time in combination with a high order parameter (S = 0.92) indicates that motilin has a rigid structure in the complex with the SDS micelle.
authors.affiliation: Department of Biophysics, Arrhenius Laboratories, Stockholm University, S-106 91 Stockholm, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Jarvet
authors.forename: J
authors.initials: J
authors.lastname: Zdunek
authors.forename: P
authors.initials: P
authors.lastname: Damberg
authors.forename: A
authors.initials: A
authors.lastname: Gräslund
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008823:Micelles; D008969:Molecular Sequence Data; D009037:Motilin / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011522:Protons; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013552:Swine
chemical_list: D008823:Micelles; D011522:Protons; D012967:Sodium Dodecyl Sulfate; D009037:Motilin