An unusual artefact in the terminal deoxynucleotidyl transferase assay for apoptotic cells.
A staining artefact associated with the terminal deoxynucleotidyl assay for apoptotic cells is described. the Artefact is caused by particulate matter in the reconstituted dried milk used in the washing buffer. We recommend filtering the washing buffer before use.
Detre
S
S
Academic Department of Biochemistry, Royal Marsden NHS Trust, London, United Kingdom.
Ormerod
M G
MG
Titley
J C
JC
Dowsett
M
M
D000818:Animals; D017209:Apoptosis; D016477:Artifacts; D002021:Buffers; D002478:Cells, Cultured; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D005434:Flow Cytometry; D051379:Mice; D008892:Milk; D013194:Staining and Labeling
D002021:Buffers; D004253:DNA Nucleotidylexotransferase