Structural analysis of disaccharides synthesized by beta-D-glucosidase of Bifidobacterium breve clb and their assimilation by Bifidobacteria.
Circulation of a solution of 1 M D-fucose and 1 M D-glucose through a reaction system consisting of serial columns of immobilized recombinant beta-D-glucosidase of Bifidobacterium breve clb and activated charcoal gave two oligosaccharides. Structural analysis identified these oligosaccharides as D-fucosylglucose (6-O-beta-D-Fucopyranosyl-D-glucose) and gentiobiose (6-O-beta-D-Glucopyranosyl-D-glucose). The D-fucosylglucose obtained was well assimilated by many Bifidobacteria but not by the other intestinal bacteria tested.
Nunoura
N
N
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Fujita
T
T
Ohdan
K
K
Kirihata
M
M
Yamamoto
K
K
Kumagai
H
H
D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001644:Bifidobacterium / Q000201:enzymology* / Q000378:metabolism; D002606:Charcoal / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004187:Disaccharides / Q000096:biosynthesis* / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology; D009682:Magnetic Resonance Spectroscopy; D016339:Spectrometry, Mass, Fast Atom Bombardment; D001617:beta-Glucosidase / Q000378:metabolism*
C107969:6-O-fucopyranosylglucose; D004187:Disaccharides; D004800:Enzymes, Immobilized; D002606:Charcoal; D001617:beta-Glucosidase; C100052:gentiobiose