LTW4 protein on mouse chromosome 1 is a member of a family of antioxidant proteins.
Based on its map position, polymorphism pattern, and expression in the kidney, the gene encoding liver 20,000-30,000 MW protein 4 (LTW4) can be considered a potential candidate for the Jckm2 modifying locus, which mediates the severity of polycystic kidney disease in the juvenile cystic kidney mouse. Using two-dimensional gel electrophoresis, we identified variants of a 26-kDa polypeptide that differed in their isoelectric points between the C57BL/6J and the DBA/2J inbred strains in a pattern similar to that originally described for LTW4 protein. N-terminal amino acid sequence was obtained by microsequencing analysis, and full-length clones were obtained by RT-PCR amplification and characterized. The map position of the cloned gene was determined and corresponded to that previously described for Ltw4. The gene has homology to a class of proteins characterized as thiol-specific antioxidants that are protective against damage caused by oxidative stress. The murine MER5 gene is also a member of this gene family and has recently been renamed Antioxidant protein 1 (Aop1), based on its functional characterization. We therefore propose that the gene encoding LTW4 be called Aop2.
Iakoubova
O A
OA
Genetics Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Pacella
L A
LA
Her
H
H
Beier
D R
DR
D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants; D001483:Base Sequence; D018076:DNA, Complementary; D015180:Electrophoresis, Gel, Two-Dimensional; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010544:Peroxidases; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; D018807:Polymorphism, Single-Stranded Conformational; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D000975:Antioxidants; D018076:DNA, Complementary; D011506:Proteins; D010544:Peroxidases; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; C515983:Prdx6 protein, mouse