Semi-micro solid-phase extraction of organic compounds from aqueous and biological samples.
A technique is described for performing solid-phase extractions on a semi-micro scale. Thin membrane disks 4 mm in diameter containing lightly sulfonated polystyrene or Silicalite particles are placed in the hub of a syringe needle. Aqueous samples (1-6 ml) are passed through the membrane disks and extracted compounds are subsequently eluted with 20-50 microliters of an organic solvent. Unlike solid-phase micro extraction (SPME) which uses a coated fiber, the present method is essentially a total extraction technique. Recoveries > 90% were generally obtained for a wide variety of test compounds. The same test compounds in human urine, albumin and human serum samples can be extracted without any pretreatment other than addition of a suitable surfactant. A "double-pass" technique was developed for convenient field sampling.
Mayer
D L
DL
Department of Chemistry, Iowa State University, Ames, USA.
Fritz
J S
JS
D000085:Acetates / Q000032:analysis; D000438:Alcohols / Q000032:analysis; D000447:Aldehydes / Q000032:analysis; D001826:Body Fluids / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D008832:Microchemistry; D008863:Microspheres; D011137:Polystyrenes; D012709:Serum Albumin / Q000032:analysis; D012996:Solutions; D013451:Sulfonic Acids; D014867:Water / Q000032:analysis*
D000085:Acetates; D000438:Alcohols; D000447:Aldehydes; D008567:Membranes, Artificial; D011137:Polystyrenes; D012709:Serum Albumin; D012996:Solutions; D013451:Sulfonic Acids; D014867:Water