title: Fetal rat brain injury: effect of transient maternal hypoxemia.
abstract: OBJECTIVE
  To determine whether transient acute maternal hypoxemia during the end of pregnancy may cause neuronal damage in fetal rat brains.
  
  
  METHODS
  Nine pregnant rats (4 study and 5 controls) at 16-17 gestational days were studied. The study rats were placed in a chamber and breathed a gas mixture of 11.8% oxygen, 4.95% CO2, and nitrogen for either 1 or 2 h, while the control animals breathed room air. Tail venous blood was collected and gases were evaluated at the beginning and conclusion of the exposure periods. After 72 h of recovery, at 19-20 days' gestation, the fetal cardiovascular systems were perfused with saline and formalin. The brains were embedded in paraffin, sectioned in coronal plane, and stained with hematoxylin and eosin Histologic assessment of sections was performed by a neuropathologist blinded to the protocol. Statistical analysis of the data was performed using analysis of variance and the chi-square test.
  
  
  RESULTS
  Exposure to the gas mixture resulted in decreased maternal pO2 from 39.9 +/- 7.6 mm Hg in the control group to 28.8 +/- 2.0 mm Hg in the 2-hour hypoxia group (p < 0.05). No significant changes in maternal pH or pCO2 status have been noted. A total of 34 fetal rat brains served as controls and 26 brains as the hypoxia study group There was a significant increase in isolated neuronal damage, including necrosis and shrinkage of cells, with karyorrhexis (fragmentation and breakage of the nucleus) in the hippocampus, basal ganglia, thalamus, and hypothalamus in the hypoxemia rats as compared with controls.
  
  
  CONCLUSIONS
  Transient maternal hypoxemia may cause neuronal necrosis in vulnerable regions of the fetal rat brain, including the hippocampus, basal ganglia, thalamus, and hypothalamus.
authors.affiliation: Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Mich., USA.
authors.forename: M
authors.initials: M
authors.lastname: Hallak
authors.forename: W J
authors.initials: WJ
authors.lastname: Kupsky
authors.forename: J W
authors.initials: JW
authors.lastname: Hotra
authors.forename: S M
authors.initials: SM
authors.lastname: Irtenkauf
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000473:pathology*; D001930:Brain Injuries / Q000196:embryology* / Q000473:pathology; D005260:Female; D000860:Hypoxia / Q000150:complications*; D018811:Maternal Exposure / Q000009:adverse effects*; D011247:Pregnancy; D011248:Pregnancy Complications; D051381:Rats; D012016:Reference Values