Endogenous ADP-ribosylation of a G(alpha i) protein in bovine ciliary body is stimulated by nitric oxide.
Five ciliary body membrane proteins were labeled when incubated with (adenylate-32P)NAD. Nitric oxide donors stimulated the labeling of 64, 40, and 29-30 kDa proteins and inhibited that of 58 and 56 kDa proteins. The greatest influence of nitric oxide was on the 40 kDa protein: a 17-fold stimulation. Western blotting and immunoprecipitation with specific antibodies identified this protein as the alpha-subunit of G(i-1). Studies with inhibitors showed that the protein was mono-ADP-ribosylated. Treatment of (32P)NAD-labeled G(i-1) with Hg and analysis of the released radioactive material showed that the protein was ADP-ribosylated on a cysteine residue.
Pozdnyakov
N
N
Eye Research Institute, Oakland University, Rochester, Michigan 48309, USA.
Margulis
A
A
Sitaramayya
A
A
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002924:Ciliary Body / Q000187:drug effects / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008628:Mercury / Q000494:pharmacology; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism*; D009569:Nitric Oxide / Q000494:pharmacology*; D009589:Nitrogen Oxides; D010761:Phosphorus Radioisotopes; D013096:Spermine / Q000031:analogs & derivatives* / Q000494:pharmacology
D006898:Hydroxylamines; D046911:Macromolecular Substances; D008565:Membrane Proteins; D009589:Nitrogen Oxides; D010761:Phosphorus Radioisotopes; D009243:NAD; C091861:spermine nitric oxide complex; D000246:Adenosine Diphosphate Ribose; D019811:Hydroxylamine; D013096:Spermine; D009569:Nitric Oxide; D019204:GTP-Binding Proteins; D008628:Mercury