title: TAP-dependent major histocompatibility complex class I presentation of soluble proteins using listeriolysin.
abstract: Immunization of mice with mixtures of listeriolysin, a pore-forming hemolysin secreted by the pathogenic bacterium Listeria monocytogenes, together with soluble ovalbumin, nucleoprotein of influenza virus, or beta-galactosidase of Escherichia coli, resulted in strong cytotoxic CD8 T cell responses to each of the respective passenger proteins in vivo. Also, the concomitant addition of either protein with listeriolysin to target cells elicited efficient sensitization of these cells which could be attributed to the pore-forming activity of listeriolysin. This response was dependent upon a functional TAP transporter and was inhibitable by brefeldin A, indicating the transfer of the soluble proteins into the cytosol and the classical major histocompatibility (MHC) class I presentation pathway. The treatment of target cells with listeriolysin under our experimental conditions did not affect cell viability and the pores generated by listeriolysin treatment were repaired within 60 min. Introduction of soluble proteins into the MHC class I presentation pathway by listeriolysin provides a powerful system to study the cytotoxic response towards intracellular pathogens and would allow for rapid screening of potential antigens in vaccine formulations.
authors.affiliation: Division of Cell Biology and Immunology, National Center for Biotechnology, Braunschweig, Germany. ada@gbf-braunschweig.de
authors.forename: A
authors.initials: A
authors.lastname: Darji
authors.forename: T
authors.initials: T
authors.lastname: Chakraborty
authors.forename: J
authors.initials: J
authors.lastname: Wehland
authors.forename: S
authors.initials: S
authors.lastname: Weiss
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000502:physiology*; D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000378:metabolism; D001427:Bacterial Toxins; D002451:Cell Compartmentation / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D003600:Cytosol / Q000276:immunology / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D011992:Endosomes / Q000276:immunology / Q000378:metabolism; D005260:Female; D005805:Genes, MHC Class I / Q000276:immunology*; D006360:Heat-Shock Proteins / Q000276:immunology* / Q000378:metabolism; D006460:Hemolysin Proteins / Q000276:immunology* / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009698:Nucleoproteins / Q000276:immunology / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D012995:Solubility
chemical_list: D018528:ATP-Binding Cassette Transporters; D000956:Antigens, Viral; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D015395:Histocompatibility Antigens Class I; D009698:Nucleoproteins; D010047:Ovalbumin; C038705:hlyA protein, Listeria monocytogenes