title: [In vitro production of endothelin-1 and prostacyclin by cultured endothelial cells in the presence of polymers].
abstract: The aim of this study was to evaluate endothelin-1 and prostacyclin production by human endothelial cells cultured in the presence of polyethylene terephthalate and collagen-coated PET. Cell counting and the assay of endothelin-1 and 6-keto-prostaglandin F1 alpha, stable metabolite of prostacyclin, were carried out after 48 hour contact of the cells with the examined materials. Endothelial cell contact with uncoated PET caused a significant reduction in cell number, a significant increase in the production of endothelin-1 and a not significant increase in 6-keto-prostaglandin F1 alpha. The endothelial cell contact with collagen-coated PET caused a highly significant decrease in cell number and a not significant decrease in endothelin-1 and 6-keto-prostaglandin F1 alpha. It was concluded that PET causes both a decrease in cell number and a remarkable increase in endothelin-1. On the contrary, collagen-coated PET determines a decrease in cell number and a slight reduction of endothelin-1 and 6-keto-prostaglandin F1 alpha.
authors.affiliation: Laboratorio di Biocompatibilità dei Materiali da Impianto, Istituti Ortopedici Rizzoli, Bologna.
authors.forename: E
authors.initials: E
authors.lastname: Cenni
authors.forename: G
authors.initials: G
authors.lastname: Ciapetti
authors.forename: E
authors.initials: E
authors.lastname: Verri
authors.forename: S
authors.initials: S
authors.lastname: Gamberini
authors.forename: D
authors.initials: D
authors.lastname: Cavedagna
authors.forename: A
authors.initials: A
authors.lastname: Di Leo
authors.forename: A
authors.initials: A
authors.lastname: Pizzoferrato
mesh_terms: D002478:Cells, Cultured; D003094:Collagen; D019332:Endothelin-1 / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D011093:Polyethylene Terephthalates
chemical_list: D019332:Endothelin-1; D011093:Polyethylene Terephthalates; D003094:Collagen; D011464:Epoprostenol