title: Analysis of the fusion protein gene of the porcine rubulavirus LPMV: comparative analysis of paramyxovirus F proteins.
abstract: Complementary DNA clones representing the fusion (F) protein gene of the porcine rubulavirus LPMV were isolated and sequenced. The F gene was found to be 1,845 nucleotides long containing one long open reading frame capable of encoding a protein of 541 amino acids. The cleavage motif for F0 into F1 and F2 is His-Arg-Lys-Lys-Arg. A sequence comparison and a phylogenetic analysis was performed in order to identify possible functional domains of paramyxovirus fusion proteins and also to classify the porcine rubulavirus. The F gene of LPMV is most closely related to the human mumps virus and simian virus type 5 F genes, and is therefore classified into the rubulavirus genus. A coding region for a small hydrophobic protein was however not found between the F and hemagglutinin-neuraminidase (HN) genes as previously found in both SV5 and mumps.
authors.affiliation: Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Biomedical Center, Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Berg
authors.forename: A C
authors.initials: AC
authors.lastname: Bergvall
authors.forename: M
authors.initials: M
authors.lastname: Svenda
authors.forename: A
authors.initials: A
authors.lastname: Sundqvist
authors.forename: J
authors.initials: J
authors.lastname: Moreno-López
authors.forename: T
authors.initials: T
authors.lastname: Linné
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000235:genetics; D016366:Open Reading Frames; D018098:Paramyxovirinae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D019213:Rubulavirus / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014760:Viral Fusion Proteins / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D014760:Viral Fusion Proteins