Characterization of iridovirus IV1 polypeptides: mapping by surface labelling.
A comprehensive index of IV1(Tipula iridescent virus, or TIV)-associated polypeptides has been established using enrichments of "empty" and "filled" virions that are considered to be maturation-phase-related. The mapping strategy which involved one- and two-dimensional polyacrylamide gel electrophoresis, silver staining, disulphide bond reduction and 125I iodination of putative surface proteins revealed 103 and 116 polypeptides for empty and filled capsids, respectively. These estimates could be reduced to < or = 70 by reclassing multicharged polypeptides of approximately identical masses as single entitles. At least 10 polypeptides from empty and filled virions were involved in intermolecular sulphhydryl linkages and another 11 species were identified as putative outer shell (surface) polypeptides. These data provide useful criteria for iridovirus classification and identification of candidate polypeptides involved in capsid formation and maturation.
Watson
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Institute of Biological Sciences, Carleton University, Ottawa, Ontario, Canada.
Seligy
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VL
D000818:Animals; D002213:Capsid / Q000032:analysis; D002700:Chloramines / Q000737:chemistry; D004175:Diptera / Q000821:virology; D004220:Disulfides; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes; D017994:Iridovirus / Q000737:chemistry*; D007553:Isotope Labeling; D007784:Lactoperoxidase / Q000737:chemistry; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D014105:Tosyl Compounds / Q000737:chemistry; D014759:Viral Envelope Proteins / Q000032:analysis*
D002700:Chloramines; D004220:Disulfides; D007457:Iodine Radioisotopes; D010455:Peptides; D014105:Tosyl Compounds; D014759:Viral Envelope Proteins; C016300:chloramine-T; D012967:Sodium Dodecyl Sulfate; D007784:Lactoperoxidase