Transcription of the Leishmania major Hsp70-I gene locus does not proceed through the noncoding region.
Primary transcripts in kinetoplastid protozoa are generally assumed to be multicistronic. We have analyzed the transcription in the gene locus which encodes the 70-kDa heat shock protein by using nuclear run-on analysis. We find that RNA synthesis in the Hsp70-I gene locus either is terminated or pauses within the intergenic region approximately 250 nt downstream of the polyadenylation site. We therefore propose a discontinuous mode of transcription in the Hsp70 genes of Leishmania major.
Dresel
A
A
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Clos
J
J
D000818:Animals; D017125:Genes, Protozoan; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics; D018320:Leishmania major / Q000235:genetics*; D005810:Multigene Family; D016366:Open Reading Frames; D015800:Protozoan Proteins / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism; D015347:RNA Probes / Q000737:chemistry; D016053:RNA, Protozoan / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D014158:Transcription, Genetic
D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C490310:Hsp83 protein, protozoan; D015800:Protozoan Proteins; D015347:RNA Probes; D016053:RNA, Protozoan; D012319:RNA Polymerase II; D015719:Single-Strand Specific DNA and RNA Endonucleases