Simultaneous determination of imipramine, desipramine and their 2- and 10-hydroxylated metabolites in human plasma and urine by high-performance liquid chromatography.
A simultaneous assay for imipramine, desipramine and their 2- and 10-hydroxy-metabolites using high-performance liquid chromatography (HPLC) is described. The drugs and internal standard, pericyazine, were extracted from plasma or urine at pH 9.6 with diethyl ether and back-extracted into 0.1 M orthophosphoric acid. The recovery of the compounds ranged from 78.6% for imipramine to 94.3% for 2-hydroxydesipramine. The extracts were analysed by reversed-phase HPLC with electrochemical detection using a mobile phase of 30% acetonitrile in 0.1 M K2HPO4 at pH 6.0 delivered at 2 ml/min. All compounds were resolved in a run time of 15 min with lower limits of quantification of 1.5 ng/ml for hydroxy-metabolites and 3 ng/ml for imipramine and desipramine. The intra- and inter-day coefficients of variation at 50 ng/ml were 5.2% and 6.8%, respectively (n=8).
Chen
A G
AG
School of Pharmacy and Chemistry, Liverpool John Moores University, UK.
Wing
Y K
YK
Chiu
H
H
Lee
S
S
Chen
C N
CN
Chan
K
K
D000328:Adult; D000929:Antidepressive Agents, Tricyclic / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D003891:Desipramine / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000652:urine*; D005260:Female; D006801:Humans; D007099:Imipramine / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000652:urine*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
D000929:Antidepressive Agents, Tricyclic; C033803:2-hydroxyimipramine; C107212:10-hydroxydesipramine; C107213:10-hydroxyimipramine; D007099:Imipramine; D003891:Desipramine; C028653:2-hydroxydesipramine