Intracellular Ca2+ imaging of cultured chicken telencephalic cells: characterization of ionotropic glutamate receptor activation.
In the present study we investigated the intracellular [Ca2+] increasing effect of the excitatory amino acid agonists kainate, AMPA and NMDA on fura-2/AM loaded chicken telencephalic cells in various conditions. Kainate (110 microM) increased [Ca2+]i to 256 +/- 23% of the basal level (n = 7). In Ca(2+)-free medium the effect of kainate on intracellular Ca2+ was completely abolished indicating that the primary source of the Ca2+ signal was the extracellular pool. Voltage dependent Ca2+ channel antagonism by Cd2+ decreased the intracellular Ca2+ elevation caused by 100 microM kainate indicating the involvement of voltage dependent Ca2+ channels (VDCC).
Lendvai
B
B
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
Mike
A
A
Zelles
T
T
Vizi
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ES
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D005456:Fluorescent Dyes; D016257:Fura-2; D007425:Intracellular Membranes / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology; D008858:Microscopy, Phase-Contrast; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism*; D017470:Receptors, Glutamate / Q000187:drug effects / Q000502:physiology*; D013687:Telencephalon / Q000166:cytology / Q000378:metabolism*
D005456:Fluorescent Dyes; D017470:Receptors, Glutamate; D016202:N-Methylaspartate; D007608:Kainic Acid; D002118:Calcium; D016257:Fura-2