[Studies on biological characteristics of HL-60 and all-trans-retinoic acid resistant HL-60 cells--comparison of the basic biological and cytogenic features of the two cell lines of HL-60].
The biological features of HL-60 and all-trans-retinoic acid resistant HL-60 cells (HL-60/ RA) were studied in terms of cell proliferation, cytokinetics, chromosome distribution and G-band karyotypes. Results indicated that the two cell lines exhibited similar morphology and doubling time and similar cytokinetic features, but the cell population in S phase of HL-60/RA is smaller than that of HL-60 cells. Their chromosome number is around 45 +/- 3 for HL-60 and 45 +/- 1 for HL-60/RA cells. G-band karyotype analysis showed that they have a similar diploid karyotype and some abnormal chromosomes. These results demonstrated that HL-60 and HL-60/RA cell lines were originated from the same source.
Li
L
L
Institute of Materia Medica, CAMS, Beijing.
Han
R
R
D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002871:Chromosome Banding; D004171:Diploidy; D019008:Drug Resistance, Neoplasm; D018922:HL-60 Cells / Q000166:cytology; D006801:Humans; D007621:Karyotyping; D016196:S Phase; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin