Effect of nitrite on blood pressure in anaesthetized and free-moving rats.
The effect of nitrite on blood pressure and heart rate was studied in anaesthetized (non-telemetric method) and free-moving rats (biotelemetry system). In anaesthetized rats, NaNO2 (10-1000 mumol/kg), infused over 5 min, induced a dose-related decrease in blood pressure. The maximal decrease in mean arterial blood pressure (MAP), caused by 1000 mumol/kg NaNO2 and measured 15 min after infusion was 55.9 +/- 3.2% (n = 3). After NaNO2 infusion, in the plasma, rapid conversion of nitrite into nitrate was observed. However, sodium nitrate (NaNO3, 100 mumol/kg) did not decrease blood pressure and there was no conversion of nitrate into nitrite. Free-moving rats received KNO2 which was added to drinking water (36 mmol/litre) for a period of 3 days. KNO2 decreased the MAP and increased the heart rate during the rat's activity phase at night but not during their resting phase in the day. An equal concentration of potassium (KCl, 36 mmol/litre added to drinking water) for 3 days did not decrease blood pressure. It is concluded that nitrite decreases blood pressure in rats, which probably induces, by renin-angiotensin system activation, hypertrophy of the adrenal zona glomerulosa.
Vleeming
W
W
RIVM National Institute of Public Health and Environment, Laboratory of Effects Research, Bilthoven, The Netherlands.
van de Kuil
A
A
te Biesebeek
J D
JD
Meulenbelt
J
J
Boink
A B
AB
D000768:Anesthesia, General; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005520:Food Preservatives / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D009068:Movement / Q000502:physiology*; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012977:Sodium Nitrite / Q000494:pharmacology*; D014851:Wakefulness
D005520:Food Preservatives; D009566:Nitrates; D009573:Nitrites; C041349:potassium nitrite; D012977:Sodium Nitrite