Solution conformation and dynamics of a tetrasaccharide related to the Lewix(X) antigen deduced by 1H NMR NOESY, ROESY, and T-ROESY measurements.
The conformational and dynamical features of a Le(X) tetrasaccharide analogue GalNAc (alpha 1-3)Gal(beta 1-4)[Fuc(alpha 1-3)]Glc(beta OMe) 1 have been studied through 1H NMR relaxation measurements. The results indicate that the different glycosidic linkages of 1 present distinct conformational flexibility in solution. In addition, the use of T-ROESY experiments in conformational analysis of oligosaccharides is explored emphasizing its scope and limitations.
Poveda
A
A
Servicio Interdepartamental de Investigación, Universidad Autónoma de Madrid, Spain.
Asensio
J L
JL
Martín-Pastor
M
M
Jiménez-Barbero
J
J
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D016256:Lewis X Antigen / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*
D016256:Lewis X Antigen; D009844:Oligosaccharides