Effects of amylin on human osteoblast-like cells.
Amylin has been reported to have bone-conserving effects. In the present study we evaluated the possible activity of the peptide on human osteoblast-like (hOB) cells in primary culture. Amylin between 10(-9) and 10(-6) M, dose-dependently stimulated cell proliferation with a maximal effect (200%) at 10(-6) M. In addition, amylin increased osteocalcin production when hOB cells were exposed to 1,25(OH)2D3 (10(-8)M) but there was a nonsignificant upward trend on alkaline phosphatase activity. The present results suggest that amylin could be included among the group of peptides endowed with osteogenic activity.
Villa
I
I
Bone Metabolic Unit, Scientific Institute H San Raffaele, Milano, Italy.
Rubinacci
A
A
Ravasi
F
F
Ferrara
A F
AF
Guidobono
F
F
D000682:Amyloid / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D006801:Humans; D058228:Islet Amyloid Polypeptide; D010006:Osteoblasts / Q000187:drug effects*
D000682:Amyloid; D058228:Islet Amyloid Polypeptide