Effects on complement, granulocytes and platelets of a leukocyte-depletion filter during in vitro extracorporeal circulation.
In an in vitro study, extracorporeal circuits equipped with either a leukocyte-depleting filter (n = 5) or a standard arterial-line filter (n = 5) were perfused for 120 minutes with fresh human whole blood. Leukocyte activation, leukocyte and platelet counts and complement activation were studied. Significant reduction of leukocyte and platelet counts and significant activation of leukocytes and of platelets were found in both groups, but without significant intergroup difference for any parameter after 120 minutes of perfusion. The leukocyte-depleting filters, however, were somewhat more effective in removing leukocytes during the initial 30 minutes of circulation.
Baksaas
S T
ST
Surigical Dept A, National Hospital, University of Oslo, Norway.
Videm
V
V
Mollnes
T E
TE
Pedersen
T
T
Karlsen
H
H
Svennevig
J L
JL
D003165:Complement System Proteins / Q000502:physiology*; D001026:Coronary Artery Bypass / Q000009:adverse effects* / Q000295:instrumentation; D006098:Granulocytes / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007958:Leukocyte Count; D008851:Micropore Filters; D010976:Platelet Count; D012016:Reference Values
D003165:Complement System Proteins