HTLV-I provirus in the clinical subtypes of ATL.
Adult T cell leukemia (ATL) is an aggressive neoplasm of mature helper T cell, which is etiologically linked with human T-lymphotropic virus type 1 (HTLV-I). We studied HTLV-I provirus in 61 cases of ATL with Southern blot analyses and long PCR. These methods detected defective virus in 34 cases (56%). Furthermore, it found two types of defective virus. The first type (type 1) defective virus had both LTRs, but lacked internal sequences, such as gag and pol. Type 1 defective virus was seen in 50% of all defective virus. The second form (type 2) of defective virus had only one LTR, and 5'-LTR was preferentially deleted. This type of defective virus could be more frequently detected in aggressive types of ATL (16/44 cases) than chronic type (1/17 cases). This defective virus might be associated with clinical subtype.
Matsuoka
M
M
Tamiya
S
S
Takemoto
S
S
Yamaguchi
K
K
Takatsuki
K
K
D000328:Adult; D015139:Blotting, Southern / Q000379:methods; D003673:Defective Viruses / Q000302:isolation & purification; D015750:Genes, gag; D015751:Genes, pol; D015368:Human T-lymphotropic virus 1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D015458:Leukemia, T-Cell / Q000145:classification* / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011533:Proviruses / Q000302:isolation & purification*; D012091:Repetitive Sequences, Nucleic Acid; D006377:T-Lymphocytes, Helper-Inducer