Evolution of phosphagen kinase V. cDNA-derived amino acid sequences of two molluscan arginine kinases from the chiton Liolophura japonica and the turbanshell Battilus cornutus.
The cDNAs of arginine kinases from the chiton Liolophura japonica (Polyplacophora) and the turbanshell Battilus cornutus (Gastropoda) were amplified by polymerase chain reaction (PCR), and the complete nucleotide sequences of 1669 and 1624 bp, respectively, were determined. The open reading frame for Liolophura arginine kinase is 1050 nucleotides in length and encodes a protein with 349 amino acid residues, and that for Battilus is 1077 nucleotides and 358 residues. The validity of the cDNA-derived amino acid sequence was supported by chemical sequencing of internal tryptic peptides. The molecular masses were calculated to be 39,057 and 39,795 Da, respectively. The amino acid sequence of Liolophura arginine kinase showed 65-68% identity with those of Battilus and Nordotis (abalone) arginine kinases, and the homology between Battilus and Nordotis was 79%. Molluscan arginine kinases also show lower, but significant homology (38-43%) with rabbit creatine kinase. The sequences of arginine kinases could be used as a molecular clock to elucidate the phylogeny of Mollusca, one of the most diverse animal phyla.
Suzuki
T
T
Department of Biology, Faculty of Science, Kochi University, Japan. suzuki@cc.kochi-u.ac.jp
Ban
T
T
Furukohri
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001122:Arginine Kinase / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D003402:Creatine Kinase / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry*; D008969:Molecular Sequence Data; D008974:Mollusca / Q000201:enzymology* / Q000235:genetics; D016366:Open Reading Frames; D010446:Peptide Fragments / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011817:Rabbits; D017385:Sequence Homology; D014357:Trypsin / Q000378:metabolism
D018076:DNA, Complementary; D010446:Peptide Fragments; D003402:Creatine Kinase; D001122:Arginine Kinase; D014357:Trypsin