Inactivation of Bacillus spores by the supercritical carbon dioxide micro-bubble method.
Bacillus spores were effectively inactivated by the supercritical (SC) CO2 micro-bubble method. The micro-bubble SC CO2 treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40 degrees C and 30 MPa for 30 min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO2 treatment of B. polymyxa, B. cereus, and B. subtilis spores at 45 degrees C, 50 degrees C, respectively, and 30 MPa for 60 min resulted in a 6-log cycle reduction of survival. The SC CO2 treatment under the foregoing conditions should offer higher efficiency than that of heat treatment at 100 degrees C for 60 min. In addition, the SC CO2 treatment (30 MPa, 60 degrees C, 30 min) of B. polymyxa and B. cereus spores also produced a 6-log cycle reduction.
Ishikawa
H
H
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Shimoda
M
M
Tamaya
K
K
Yonekura
A
A
Kawano
T
T
Osajima
Y
Y
D001407:Bacillus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002245:Carbon Dioxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D011312:Pressure; D013171:Spores, Bacterial / Q000187:drug effects; D013242:Sterilization / Q000379:methods* / Q000592:standards; D013696:Temperature
D002245:Carbon Dioxide