Activity of antioxidant defense enzymes and glutathione content in some tissues of the Belgrade (b/b) laboratory rat.
The activity of antioxidant defense (AD) enzymes--superoxide dismutase (SOD, EC 1.15.1.1.), catalase (CAT, EC 1.11.1.6.), glutathione peroxidase (GSH-Px, EC 1.11.1.9.), glutathione-S-transferase (GST, EC 2.5.1.18), glutathione reductase (GR, EC 1.6.4.2) and glutathione (GSH) content of the anemic Belgrade (b/b) laboratory rats--were measured and analyzed in liver, spleen, lung, heart, brain and testes in comparison with nonanemic controls. The activities of hepatic Mn SOD, CAT, GSH-Px and GST (P < 0.02, P < 0.01 and P < 0.005) were decreased in anemic, comparing with nonanemic animals, whereas the spleen CuZn SOD, Mn SOD, CAT and GSH-Px (P < 0.005, P < 0.02, P < 0.005 and P < 0.01) activities were increased. In the lung of anemic rats, Mn SOD, GSH-Px and GR (P < 0.005, P < 0.01, P < 0.05) activities were higher, whereas GST (P < 0.01) activity was lower in relation to nonanemic ones. In anemic rats, heart Mn SOD (P < 0.05) activity was increased, brain GSH-Px (P < 0.005) activity was lower, whereas GR (P < 0.02) activity was higher compared with nonanemic controls. CuZn SOD (P < 0.05) activity in the testes was elevated and GSH-Px (P < 0.05) reduced in anemic animals. GSH content was decreased in the liver (P < 0.01), lung and brain (P < 0.005) and increased in the spleen (P < 0.02) of anemic rats in relation to the controls. Our data suggest phenotype specific differences in the AD system of the Belgrade (b/b) rat tissues in comparison with nonanemic controls.
Saicić
Z S
ZS
Department of Physiology, Institute for Biological Research Sinisa Stanković, Serbia, Yugoslavia.
Simović
M O
MO
Korać
B
B
Blagojević
D
D
Buzadzić
B
B
Spasić
M B
MB
Petrović
V M
VM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D011913:Rats, Inbred BB / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology; D013737:Testis / Q000201:enzymology
D000975:Antioxidants; D010088:Oxidoreductases; D005978:Glutathione