Effects of alveolar macrophage conditioned media from interstitial lung disease patients on the procollagen mRNA expression in human lung fibroblasts.
Progressive inflammation and fibrosis are the central processes in the pathogenesis of pulmonary fibrosis. It is believed that macrophages in areas of chronically inflamed lung play a key role in fibrotic response. Therefore, we investigated the effects of alveolar macrophage (Am phi) conditioned media from interstitial lung disease (ILD) patients on lung fibroblast proliferation and procollagen mRNA expression. After stimulating with Am phi conditioned media from ILD patients, the fibroblast proliferation increased 71.4% compared with the control, but for media from bronchial carcinoma (BC) patients, it just increased 14.3%. There is a significant difference between the two groups (P < 0.05). The procollagen alpha, (I) mRNA in fibroblasts stimulated with Am phi conditioned media from ILD patients was increased 21.3%, and alpha 1 (III) was 37.2% higher than control (P < 0.05). It increased 6.8% and 12.8% for media from BC patients respectively, but there was no difference when compared to the control. We considered that Am phi from ILD patients might be in an activated state and could release some growth factors to stimulate fibroblast proliferation and promote collagen DNA expression.
Guo
Z
Z
Department of Internal Medicine, PUMC Hospital, Beijing.
Zhu
Y
Y
Lui
B
B
Zhu
Y
Y
Zhao
W
W
Chen
Y
Y
D002283:Carcinoma, Bronchogenic / Q000473:pathology; D002455:Cell Division; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005333:Fetus; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D017563:Lung Diseases, Interstitial / Q000473:pathology*; D008175:Lung Neoplasms / Q000473:pathology; D016676:Macrophages, Alveolar / Q000276:immunology*; D011347:Procollagen / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*
D017077:Culture Media, Conditioned; D011347:Procollagen; D012333:RNA, Messenger