Amino acid sequences and activities of multiple hyperglycemic hormones from the Kuruma prawn, Penaeus japonicus.
By means of a single-step reversed-phase HPLC, six CHH family peptides were isolated from sinus gland extracts of the kuruma prawn, Penaeus japonicus. Five of these peptides (Pej-SGP-I, -II, -III, -V, and -VI) expressed hyperglycemic activity and were considered to be the hyperglycemic hormones of this prawn. The amino acid sequences of the five peptides were determined (the amino acid sequence of Pej-SGP-III had been previously determined), revealing that all consisted of 72 amino acid residues with a free amino-terminus and an amidated carboxyl-terminus. They showed considerable sequence similarity to one another, but much less similarity to Pej-SGP-IV with molt-inhibiting activity, the sequence of which had also been determined previously. Examination of the dose-response relationship of these peptides showed that they were equally potent but had different efficacies, which were in the order of Pej-SGP-V, -VI > -III, -I > -II, corresponding well with their sequence characteristics.
Yang
W J
WJ
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Aida
K
K
Nagasawa
H
H
D000595:Amino Acid Sequence; D000818:Animals; D006943:Hyperglycemia / Q000503:physiopathology; D007447:Invertebrate Hormones / Q000502:physiology; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000502:physiology*; D010455:Peptides / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D007447:Invertebrate Hormones; D010455:Peptides