Nuclear translocation of constitutive heat shock protein 70 during S phase in synchronous macroplasmodia of Physarum polycephalum.
The level of constitutive heat shock protein 70 (HSC70) in Physarum polycephalum was analyzed by means of Western blots during the synchronous cell cycle of macroplasmodia. Total amounts as well as nuclear and cytoplasmic contents were determined separately and evaluated densitometrically. A drastic increase of nuclear HSC70 was observed 10-40 min after the initiation of S phase (600% of the M phase value) and thereafter a slow decline toward the next M phase. Total HSC levels showed a slight (30%) increase during S phase whereas cytoplasmic HSC70 was about 30% lower during S phase compared to mitosis.
Gevers
M
M
Institute of Cell Biology, Biochemistry and Biotechnology, University of Bremen, Germany.
Fracella
F
F
Rensing
L
L
D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D010804:Physarum / Q000378:metabolism*; D016196:S Phase
D018840:HSP70 Heat-Shock Proteins