The repertoire of T cell antigen receptor beta-chain variable regions associated with psoriasis vulgaris.
We investigated whether the pattern of T-cell receptors expressed by T cells in inflamed psoriatic skin differed substantially from the pattern seen in T cells from the peripheral blood. A bias or restriction in the repertoire of T-cell receptors found in the lesional skin of different patients might imply that specific subsets of T cells were causally associated with initiating or maintaining the lesions. By using a polymerase chain reaction-based assay of T-cell receptor beta-chain variable region mRNA, we found that the patterns of beta-chain mRNAs displayed in 14 samples of lesional skin or six samples of noninvolved skin were not significantly less diverse than the patterns found in matched peripheral blood samples. There was no evidence that the active lesions of multiple patients showed overexpression of T cells expressing one or a few T-cell receptor forms. The pattern of T-cell receptors displayed in clinically normal skin from normal control individuals showed about the same diversity as normal blood. While these results may not exclude either classical antigen or superantigen-based T-cell activation mechanisms in active plaques, the absence of a simple pattern of Vbeta usage in different patients suggests than other aspects of T-cell biology including trafficking, proliferation, co-stimulation, or responses to cytokines must also be considered.
Moss
P
P
Department of Molecular Biology & Immunology, Darwin Molecular Corporation, Bothell, Washington 98021, U.S.A.
Charmley
P
P
Mulvihill
E
E
Ziegler
S
S
Raugi
G J
GJ
Kern
D
D
Piepkorn
M
M
Gelinas
R
R
D017931:DNA Primers / Q000032:analysis; D015395:Histocompatibility Antigens Class I / Q000097:blood; D006650:Histocompatibility Testing; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011565:Psoriasis / Q000097:blood / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D012867:Skin / Q000737:chemistry / Q000276:immunology
D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; D007135:Immunoglobulin Variable Region; D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta