title: Induction of host signal transduction pathways by Helicobacter pylori.
abstract: Adherence of Helicobacter pylori to cultured gastric epithelial cells is associated with several cellular events, including the tyrosine phosphorylation of a 145-kDa host protein; the reorganization of the host cell actin and associated cellular proteins, like vasodilator-stimulated phosphoprotein, adjacent to the attached bacterial cell; and the subsequent release of the cytokine, interleukin 8 (IL-8). H. pylori isolated from patients with ulcer disease and gastric cancer contain a DNA insertion, the cag pathogenicity island (PAI), that is not present in bacteria isolated from individuals with asymptomatic infection. Mutations in a number of PAI genes abolish tyrosine phosphorylation and IL-8 synthesis but not the cytoskeletal rearrangements. Kinase inhibition studies suggest there are two distinct pathways operative in stimulating IL-8 release from host cells and one of these H. pylori pathways is independent of the tyrosine phosphorylation step.
authors.affiliation: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: E D
authors.initials: ED
authors.lastname: Segal
authors.forename: C
authors.initials: C
authors.lastname: Lange
authors.forename: A
authors.initials: A
authors.lastname: Covacci
authors.forename: L S
authors.initials: LS
authors.lastname: Tompkins
authors.forename: S
authors.initials: S
authors.lastname: Falkow
mesh_terms: D002460:Cell Line; D004848:Epithelium / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D005753:Gastric Mucosa / Q000378:metabolism; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D015398:Signal Transduction; D013270:Stomach / Q000382:microbiology* / Q000473:pathology
chemical_list: D016209:Interleukin-8