Expression of TA1, a rat oncofetal cDNA with homology to transport-associated genes, in carbon-tetrachloride-induced liver injury.
TA1, a novel rat oncofetal cDNA, is the predicted homolog of the human lymphocyte activation gene E16. The encoded peptides share high homology with transport-associated and uncharacterized sequences in distant species, suggesting an important and conserved function in cellular homeostasis. Moderate steady-state levels of TA1 RNA were induced following acute and chronic CCl4-mediated liver injury. TA1 expression was either greatly reduced or absent in livers of animals receiving injury-protective doses of vitamin E in conjunction with CCl4. In contrast to the in vivo data, acute in vitro exposure of hepatocytes to CCl4 did not induce TA1 RNA. Our results indicate that TA1 is spatially and temporally associated with liver injury in vivo and may play an adaptive role in the hepatic response to environmental toxicants.
Shultz
V D
VD
Department of Medical Oncology, Rhode Island Hospital/Brown University, Providence 02903, USA.
Degli Esposti
S
S
Panzica
M A
MA
Abraham
A
A
Finch
P
P
Thompson
N L
NL
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics; D001692:Biological Transport; D002252:Carbon Tetrachloride Poisoning / Q000235:genetics*; D018076:DNA, Complementary; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D017403:In Situ Hybridization; D027283:Large Neutral Amino Acid-Transporter 1; D008099:Liver / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D027283:Large Neutral Amino Acid-Transporter 1; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C000632119:Slc7a5 protein, rat; C031192:oncofetal antigens