[The isolation amd study of lipopolysaccharide from Klebsiella pneumoniae vaccinal strain 204].
K. pneumoniae lipopolysaccharide (LPS) was isolated from biomass grown under the conditions of controlled multicycle and continuous cultivation. A considerable yield of LPS containing the minimal amount of protein and nucleic acid admixtures was obtained from biomass grown by continuous cultivation with the concentration of glucose in the medium equal to 20 g/l and the content of dissolved oxygen at a level of 0% of complete saturation. Erythrocyte diagnosticum prepared on the basis of this LPS was found to have a sensitizing dose of 100 micrograms per ml of solid erythrocytic precipitate and high specificity, studied in the passive hemagglutination test with the use of rabbit sera.
Stukalova
N V
NV
Karabak
V I
VI
Aleksakhina
N N
NN
Basnak'ian
I A
IA
Miriasova
L V
LV
D000818:Animals; D001428:Bacterial Vaccines; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000276:immunology; D007114:Immunization / Q000379:methods; D007710:Klebsiella Infections / Q000175:diagnosis; D007711:Klebsiella pneumoniae; D007928:Lethal Dose 50; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D051379:Mice; D011817:Rabbits; D012680:Sensitivity and Specificity
D001428:Bacterial Vaccines; D003470:Culture Media; D008070:Lipopolysaccharides