Identification and characterization of Saccharomyces cerevisiae EXO1, a gene encoding an exonuclease that interacts with MSH2.
A two-hybrid screen was used to identify Saccharomyces cerevisiae genes encoding proteins that interact with MSH2. One gene was found to encode a homologue of Schizosaccharomyces pombe EXO1, a double-stranded DNA-specific 5'-3' exonuclease. S. cerevisiae EXO1 interacted with both S. cerevisiae and human MSH2 in two-hybrid and coimmunoprecipitation experiments. exo1 mutants showed a mutator phenotype, and epistasis analysis was consistent with EXO1 functioning in the MSH2-dependent mismatch repair pathway. exo1 mutations were lethal in combination with rad27 mutations, and overexpression of EXO1 suppressed both the temperature sensitive and mutator phenotypes of rad27 mutants.
Tishkoff
D X
DX
Division of Human Cancer Genetics, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
Boerger
A L
AL
Bertrand
P
P
Filosi
N
N
Gaida
G M
GM
Kane
M F
MF
Kolodner
R D
RD
D000595:Amino Acid Sequence; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000235:genetics*; D005092:Exonucleases / Q000235:genetics*; D005656:Fungal Proteins; D005800:Genes, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D051718:MutS Homolog 2 Protein; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C114331:EXO1 protein, human; D005090:Exodeoxyribonucleases; D005092:Exonucleases; C028450:exodeoxyribonuclease I; C489751:MSH2 protein, S cerevisiae; D051718:MutS Homolog 2 Protein; C489752:msh2 protein, S pombe; D045643:DNA Repair Enzymes