title: A study of the binding of merocyanine 540 to myeloid leukemia M1 cells using an intensified charge-coupled device for fluorescence imaging microscopy.
abstract: The binding of merocyanine 540 (MC540) to murine myeloid leukemia (M1) cells and normal erythrocytes was measured by fluorescence digital imaging microscopy using an intensified charge-coupled device. It was found that, on average, about three times more MC540 were bound to a unit membrane area of M1 cells than erythrocytes, a result consistent with previous studies. However, it was shown for the first time that MC540 binding varied significantly from one M1 cell to the next, and about 15% of the sensitized M1 cells were as MC540-negative as normal erythrocytes. Using the leukemic inhibitory factor as a differentiation inducer, M1 cells were induced to differentiate into mature macrophage-like cells in vitro. Such treatment lowered the average MC540 binding by about one-third but did not affect the cell-to-cell variation significantly.
authors.affiliation: Department of Physics, Fudan University, Shanghai, People's Republic of China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Chen
authors.forename: N K
authors.initials: NK
authors.lastname: Mak
authors.forename: W N
authors.initials: WN
authors.lastname: Leung
authors.forename: S C
authors.initials: SC
authors.lastname: Chen
authors.forename: K N
authors.initials: KN
authors.lastname: Leung
authors.forename: N H
authors.initials: NH
authors.lastname: Cheung
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000378:metabolism; D007700:Kinetics; D007951:Leukemia, Myeloid / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D010641:Phenotype; D017319:Photosensitizing Agents / Q000378:metabolism*; D011744:Pyrimidinones / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured
chemical_list: D017319:Photosensitizing Agents; D011744:Pyrimidinones; C003954:merocyanine dye