Evaluation of hemolysis in a pulsatile assist device for centrifugal pump.
To evaluate the blood trauma caused by a new device for producing a pulsatile flow of the centrifugal pump, the pulsatile assist device for the centrifugal pump (PAD-CP) that we have developed, a hemolysis study was performed in vitro and in animal experimentation. For the in vitro testing, 2 identical sets of hemolysis test circuits were prepared with 2,400 ml of bovine blood. The 2 circuits were pumped simultaneously. Plasma total hemoglobin levels were less than 40 mg/dl after 3 h, under a pump flow of 2 L/min. Hemolysis increased to a severe level after 4 h of 4 L/min pump flow. The cause of this hemolysis was thought to be a vibration of the circuit because of incomplete compression of the polyurethane tube in the PAD-CP. Five adult sheep (average body weight, 47 kg) were used for in vivo evaluation of hemolysis. Hemolysis was less than 30 mg/dl of plasma hemoglobin after 4 h of open chest extracorporeal circulation with 3.0-3.6 L/min of flow rate using the PAD-CP. Other hematologic changes after PAD-CP driving were within normal limits. We conclude that the PAD-CP has proven to have possible clinical applications.
Iwaya
F
F
Department of Cardiovascular Surgery, Fukushima Medical College, Japan.
Igari
T
T
Hoshino
S
S
Hikichi
H
H
D000818:Animals; D000925:Anticoagulants / Q000627:therapeutic use; D001783:Blood Flow Velocity; D002417:Cattle; D002498:Centrifugation; D005112:Extracorporeal Circulation / Q000592:standards; D006353:Heart-Assist Devices / Q000009:adverse effects* / Q000592:standards; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis; D006493:Heparin / Q000627:therapeutic use; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000097:blood; D010976:Platelet Count; D011140:Polyurethanes / Q000378:metabolism; D011673:Pulsatile Flow; D012756:Sheep; D013927:Thrombosis / Q000517:prevention & control
D000925:Anticoagulants; D006454:Hemoglobins; D011140:Polyurethanes; D006493:Heparin; D007770:L-Lactate Dehydrogenase