Effect of interferon-gamma on lymphocyte cell subsets in human large bowel: a study using organ culture method.
This study was conducted to investigate the effects of interferon (IFN)-gamma on normal colonic lamina propria lymphocyte subsets in humans using organ culture method. Lamina propria lymphocyte subsets in normal colonic biopsy tissues receiving 1 x 10(5) u/ml of IFN-gamma (IFN-gamma-treated group) were investigated in comparison with those cultured in medium only (IFN-gamma-non-treated group) for 24 hr. CD8-positive cells and IgG, IgA1 and IgM-containing cells were elevated in the IFN-gamma-treated group compared with those in the IFN-gamma-non-treated group, which was similar to immunological changes in mucosal lesions of inflammatory bowel disease.
Suzuki
T
T
First Department of Internal Medicine, Akita University School of Medicine.
Horie
Y
Y
Chiba
M
M
Iizuka
M
M
Masamune
O
O
D000911:Antibodies, Monoclonal; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D003106:Colon / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000276:immunology / Q000473:pathology; D007371:Interferon-gamma / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D016131:Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D011994:Recombinant Proteins; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D011994:Recombinant Proteins; D007371:Interferon-gamma