Reduced tumorigenicity of metastatic human lung cancer cell subline (PGCL3) transfected with hRAR beta gene.
The recombinant PSG5-RAR beta plasmid and the G418-resistant PSV2 neo plasmid (10.1) were cotransfected into PGCL3 cells by coprecipitation with calcium phosphate. The transfectants CR3 and CR4, which expressed the RAR beta gene, were identified by Northern blot hybridization. The results showed that the in vitro growth and invasion of CR3 and CR4 were dramatically reduced compared to the control-transfected cell (CSV1). Furthermore, the colony-forming abilities in soft agar and the tumorigenicity in nude mice of CR3 and CR4 were abrogated. Our results suggests that RAR beta functions not only as a receptor mediating the RA action, but also as a suppressor in lung tumorigenesis.
Zhu
W
W
Department of Pathology, Beijing Medical University.
Wu
B
B
Zheng
J
J
Fang
W
W
Wan
J
J
You
J
J
D016475:3T3 Cells; D000818:Animals; D018286:Carcinoma, Giant Cell / Q000473:pathology; D002461:Cell Line, Transformed; D005260:Female; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010957:Plasmids; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D018168:Receptors, Retinoic Acid