Intrathecal administration of recombinant human superoxide dismutase 1 in amyotrophic lateral sclerosis: a preliminary safety and pharmacokinetic study.
We undertook a safety and pharmacokinetic study of intrathecal (i.t.) recombinant human superoxide dismutase (rhSOD1). We administered rhSOD1 as an acute bolus in three sheep and 16 human subjects with amyotrophic lateral sclerosis (ALS). Two sheep received chronic i.t. infusion of rhSOD1 (one at 17.7 mg per day, the second at 38.0 mg per day) for six months. Two of the 16 subjects had familial ALS and mutations in the gene for Cu/Zn SOD1. They both received i.t. infusion of rhSOD1 (5 to 10 mg per day) for 3 to 6 months. Intrathecal rhSOD1 administration was safe. Bolus i.t. administration of 0.25 mg rhSOD1 in sheep revealed a mean elimination half-life of 0.4 (SD +/- 0.06) hours, clearance of 12.2 +/- 3.2 ml per hour, and volume of distribution of 7.3 +/- 0.9 ml. After chronic i.t. infusion, the initial alpha-phase half-life was estimated as 1.2 hours and the extended beta-phase half-life was 15.0 hours. The mean clearance rate was 25.9 ml per hour and the steady-state volume of distribution was 920.6 ml. Bolus i.t. administration of 20 micrograms of rhSOD1 in ALS subjects revealed a mean elimination half-life of 2.2 +/- 0.8 hours, clearance of 1.2 +/- 0.6 ml per hour, and volume of distribution of 3.5 +/- 0.4 ml. With chronic i.t. infusion of 5 mg per day, cerebrospinal SOD1 levels increased approximately fortyfold. We detected no benefit of this treatment in the two patients with familial ALS.
Cudkowicz
M E
ME
Day Neuromuscular Research Laboratory, Massachusetts General Hospital, Charlestown, USA.
Warren
L
L
Francis
J W
JW
Lloyd
K J
KJ
Friedlander
R M
RM
Borges
L F
LF
Kassem
N
N
Munsat
T L
TL
Brown
R H
RH
D000328:Adult; D000690:Amyotrophic Lateral Sclerosis / Q000188:drug therapy*; D000818:Animals; D006801:Humans; D007278:Injections, Spinal; D008875:Middle Aged; D011994:Recombinant Proteins / Q000493:pharmacokinetics; D012756:Sheep; D013482:Superoxide Dismutase / Q000008:administration & dosage* / Q000009:adverse effects* / Q000493:pharmacokinetics
D011994:Recombinant Proteins; D013482:Superoxide Dismutase