Structure of the 13C-enriched O-deacetylated glucuronoxylomannan of Cryptococcus neoformans serotype A determined by NMR spectroscopy.
The complete assignment of 1H and 13C chemical shifts for 99% uniformly 13C-labeled O-deacetylated glucuronoxylomannan (GXM) of Cryptococcus neoformans serotype A isolate 9759-Mu-1 was accomplished by the analysis of HCCH-TOCSY and HCCH-COSY spectra. The sequence of the glycosyl residues was determined by a GHMBC experiment using 20% uniformly 13C-labeled GXM; GXM was prepared by a novel procedure that insured the virtual exclusion of adjacent 13C-labeled carbon atoms. For each residue in the GXM of 9759-Mu-1 we determined its linkage position, its anomeric configuration, and its position in the repeating sequence as follows: [sequence: see text]
Sheng
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Department of Chemistry (LBCS), Georgia State University, Atlanta 30303-3083, USA.
Cherniak
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R
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003455:Cryptococcus neoformans / Q000737:chemistry* / Q000145:classification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D017421:Sequence Analysis; D012703:Serotyping
D009005:Monosaccharides; D009844:Oligosaccharides; D011134:Polysaccharides; C027478:glucuronoxylomannan