Effect of granulocyte colony-stimulating factor on the candidacidal activity of polymorphonuclear neutrophils and their collaboration with fluconazole.
The effect of granulocyte colony-stimulating factor (GCSF) treatment of polymorphonuclear neutrophils (PMN) in vitro was studied with respect to their candidacidal activity. The candidacidal activity of PMN was found to be significantly increased when they were pretreated with GCSF. Fluconazole (1 microg/ml) was found to be highly fungistatic (90%) for Candida albicans Sh27 and collaborated with PMN for significantly increased killing. Collaborative killing by PMN significantly increased when they were treated with GCSF before and after fungal exposure. The enhancing activities of GCSF required optimization of the GCSF dose and were thus inoculum and strain dependent.
Natarajan
U
U
Department of Medicine, Santa Clara Valley Medical Center, and California Institute for Medical Research, San Jose 95128-2699, USA.
Brummer
E
E
Stevens
D A
DA
D000935:Antifungal Agents / Q000627:therapeutic use*; D002176:Candida albicans / Q000187:drug effects*; D004359:Drug Therapy, Combination; D015725:Fluconazole / Q000627:therapeutic use*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D011994:Recombinant Proteins
D000935:Antifungal Agents; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D015725:Fluconazole