Adenovirus and respiratory syncytial virus in chronic sinusitis using polymerase chain reaction.
The aim of this study is to investigate the role of adenovirus and respiratory syncytial virus (RSV) in chronic sinusitis using the polymerase chain reaction (PCR) to assay for the viruses. PCR has proved to be more sensitive and specific than viral cultures and immunoassays in the detection of viruses. Adenovirus and RSV are among the most common viruses to cause upper respiratory tract infections. Sinus mucosa biopsies from 20 patients undergoing endoscopic sinus surgery were sterilely collected. Four specimens (20%) tested positive for RSV by PCR and none tested positive for adenovirus. Only one specimen tested positive for RSV and one for adenovirus by viral culture and immunofluorescence. Bacterial cultures tested positive in 40% of the 20 specimens. PCR can be used to detect RSV in patients with chronic sinusitis and PCR is more sensitive than viral culture and immunofluorescence techniques on sinus polyps and mucosa.
Ramadan
H H
HH
West Virginia University, Department of Otolaryngology-Head and Neck Surgery, Morgantown 26506-9200, USA.
Farr
R W
RW
Wetmore
S J
SJ
D000256:Adenoviridae / Q000145:classification / Q000235:genetics; D000257:Adenoviridae Infections / Q000175:diagnosis*; D000293:Adolescent; D000328:Adult; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D001706:Biopsy; D002648:Child; D002908:Chronic Disease; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004724:Endoscopy; D005455:Fluorescent Antibody Technique; D006801:Humans; D008875:Middle Aged; D009092:Mucous Membrane / Q000382:microbiology / Q000473:pathology / Q000821:virology; D010255:Paranasal Sinus Neoplasms / Q000821:virology; D016133:Polymerase Chain Reaction; D011127:Polyps / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification / Q000235:genetics; D012680:Sensitivity and Specificity; D012852:Sinusitis / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology / Q000601:surgery / Q000821:virology*; D014773:Virology / Q000379:methods
D004279:DNA, Viral