title: Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae.
abstract: Thymidylate synthase converts deoxyuridylate to deoxythymidylate. The thyA gene has been cloned and sequenced from Neisseria gonorrhoeae MS11. This gene has an open reading frame of 795-801 bp and encodes a product which shares 71% identity with its Escherichia coli homolog. Unlike its E. coli counterpart, gonococcal thyA has a large, upstream transcribed region (300+ bp) that lacks a translatable reading frame. Gonococcal thyA is capable of complementing an E. coli thyA null mutant and shares similar levels of sensitivity with trimethoprim.
authors.affiliation: Department of Health and Human Services, National Institutes of Health, National Institute of Allergy and Infectious Diseases, USA. jcarlson@pop.niaid.nih.gov
authors.forename: J H
authors.initials: JH
authors.lastname: Carlson
authors.forename: S A
authors.initials: SA
authors.lastname: Hill
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D013940:Thymidylate Synthase / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D014295:Trimethoprim / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D014295:Trimethoprim; D013940:Thymidylate Synthase