title: Inactivated poliovirus vaccine protects transgenic poliovirus receptor mice against type 3 poliovirus challenge.
abstract: Transgenic (Tg) mice expressing the human poliovirus receptor (PVR) were vaccinated with inactivated poliovirus vaccine (IPV) and evaluated for induced immunity against type 3 poliomyelitis. One injection of monovalent type 3 IPV elicited protective immunity against wild-type poliovirus. In contrast, 2 injections of trivalent IPV were required for protection. Neutralizing antibody response and protection were vaccine dose-dependent. Administration of polio-immune mouse plasma protected unimmunized mice, demonstrating that neutralizing antibody was sufficient for immunity. IPV heated to remove its D antigen component did not induce protection in Tg PVR mice. IPV derived from a wild-type poliovirus strain gave better protection against wild-type viral challenge than IPV derived from an attenuated poliovirus strain. The newly developed Tg PVR mouse-protection test may be useful in evaluating existing IPV potency tests and for attempts to improve formulations of trivalent IPV or combined vaccines for childhood immunization schedules.
authors.affiliation: Food and Drug Administration, Rockville, Maryland, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Taffs
authors.forename: Y V
authors.initials: YV
authors.lastname: Chernokhvostova
authors.forename: E M
authors.initials: EM
authors.lastname: Dragunsky
authors.forename: T
authors.initials: T
authors.lastname: Nomura
authors.forename: K
authors.initials: K
authors.lastname: Hioki
authors.forename: E C
authors.initials: EC
authors.lastname: Beuvery
authors.forename: E A
authors.initials: EA
authors.lastname: Fitzgerald
authors.forename: I S
authors.initials: IS
authors.lastname: Levenbook
authors.forename: D M
authors.initials: DM
authors.lastname: Asher
mesh_terms: D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D007116:Immunization, Passive; D008565:Membrane Proteins; D051379:Mice; D008822:Mice, Transgenic; D009500:Neutralization Tests; D011051:Poliomyelitis / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control*; D017955:Poliovirus / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000008:administration & dosage / Q000276:immunology*; D011991:Receptors, Virus / Q000235:genetics*; D015164:Vaccines, Inactivated / Q000032:analysis / Q000276:immunology
chemical_list: D008565:Membrane Proteins; D011054:Poliovirus Vaccine, Inactivated; D011991:Receptors, Virus; D015164:Vaccines, Inactivated; C066100:poliovirus receptor