Genetic studies of neuropeptide Y and neuropeptide Y receptors Y1 and Y5 regions in morbid obesity.
Synthesis and release of neuropeptide Y (NPY) are both regulated by leptin binding to its hypothalamic receptor mediating some of the effects of leptin on food intake. Moreover, NPY administration is a powerful stimulant of feeding behaviour. Thus, we investigated the potential implication of NPY, NPY-Y1 and -Y5 subtype receptors [rNPY-Y1/-Y5] in the development of human obesity. Two complementary genetic approaches were used: 1) linkage analyses between obesity and polymorphic markers located nearby NPY and rNPY-Y1/-Y5 genes (respectively on chromosomes 7p15.1 and 4q[31.3-32]) in 93 French Caucasian morbidly obese families; 2) single strand conformation polymorphism (SSCP) scanning of the coding region of the NPY and rNPY-Y1 genes performed in 50 unrelated obese patients ascertained on the basis of a body mass index of 27 kg/m2 or more and a family history of obesity. No evidence of linkage between morbid obesity or obesity-related quantitative traits and NPY and rNPY-Y1/ Y5 regions was found in this population. Moreover, SSCP scanning revealed no mutation in the coding region of NPY and rNPY-Y1 genes among obese subjects. These results suggest that NPY and NPY-Y1/ Y5 receptors are unlikely to be implicated in the development of human morbid obesity, at least in the French Caucasian population.
Roche
C
C
CNRS EP10, Institut Pasteur, Lille, France.
Boutin
P
P
Dina
C
C
Gyapay
G
G
Basdevant
A
A
Hager
J
J
Guy-Grand
B
B
Clément
K
K
Froguel
P
P
D000483:Alleles; D015992:Body Mass Index; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D002897:Chromosomes, Human, Pair 7; D017931:DNA Primers; D005091:Exons; D005602:France; D005819:Genetic Markers; D006801:Humans; D009679:Nuclear Family; D009767:Obesity, Morbid / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011964:Receptors, Gastrointestinal Hormone / Q000096:biosynthesis / Q000235:genetics*; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis / Q000235:genetics*; D044465:White People
D017931:DNA Primers; D005819:Genetic Markers; D011964:Receptors, Gastrointestinal Hormone; D017476:Receptors, Neuropeptide Y; C082172:peptide YY receptor