Structural elucidation of two polysaccharides present in the lipopolysaccharide of a clinical isolate of Burkholderia cepacia.
Based on the sugar composition, methylation analyses and Smith degradation, supported by NMR spectroscopic analyses and fast-atom-bombardment MS experiments, the lipopolysaccharide produced by a clinical isolate of Burkholderia cepacia was shown to contain two distinct polymers, both with linear trisaccharide repeating units; a major, containing D-rhamnose and D-galactose residues (2:1) with the structure -->3)-alpha-D-Rhap(1-->3)-alpha-D-Rhap(1-->4)-alpha-D-Galp(1 --> (major), and a minor repeating unit, constituted by D-rhamnosyl residues, with the structure -->3)-alpha-D-Rhap(1-->3)-alpha-D-Rhap(1-->2)-alpha-D-Rha p(1--> (minor).
Cérantola
S
S
Institut de Pharmacologie et de Biologie Structurale du CNRS, Toulouse, France.
Montrozier
H
H
D016956:Burkholderia cepacia / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment
D008070:Lipopolysaccharides