Cloning and sequencing of the cellular-viral junctions from the human adenovirus type 5 transformed 293 cell line.
The human-viral junctions of integrated adenovirus type 5 (Ad5) DNA in 293 cells have been cloned and sequenced. The Ad5 sequences extend from nucleotides (nt) 1 to 4344 and are located in the pregnancy-specific beta-1-glycoprotein 4 (PSG 4) gene. This maps the insertion of Ad5 DNA to human chromosome 19 (19q13.2). The Ad5 sequences are represented as a single collinear insertion of viral DNA with no rearrangements. There were 19 bp of PSG4 DNA deleted at the site of insertion and an extra 3 nt (GTC) at the left viral/cellular junction. A short patch of homology between viral DNA and PSG4 DNA (6 nt with 1 mismatch) was present at the right junction.
Louis
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N
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Evelegh
C
C
Graham
F L
FL
D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D016662:Virus Integration
D004279:DNA, Viral