Use of dot-immunobinding assay for visual detection of rinderpest antibodies in vaccinated cattle.
A dot-immunobinding (DIB) assay was used to detect rinderpest antibodies in cattle vaccinated with Kabete 'O' strain vaccine, using purified rinderpest virus. Of 120 serum samples from vaccinated and non-vaccinated animals, rinderpest antibodies were detected in 80%, 88.4% and 91.6% of samples at 2, 3 and 4 weeks postvaccination respectively. All the serum samples from non-vaccinated animals were negative. The DIB results had a good correlation with those of the micro neutralisation test. The technique is simple, easy to perform and suitable for routine use in detecting rinderpest antibodies following vaccination.
Singh
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G
Department of Veterinary Public Health and Epidemiology, Punjab Agricultural University, Ludhiana, India.
Singh
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I
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D015151:Immunoblotting / Q000379:methods / Q000592:standards / Q000662:veterinary*; D009500:Neutralization Tests / Q000379:methods / Q000592:standards / Q000662:veterinary; D012301:Rinderpest / Q000097:blood / Q000276:immunology* / Q000517:prevention & control; D012302:Rinderpest virus / Q000276:immunology*; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000592:standards*
D000914:Antibodies, Viral; D014765:Viral Vaccines