title: Cloning of equine type II procollagen and the modulation of its expression in cultured equine articular chondrocytes.
abstract: The complete nucleotide sequence of equine type II procollagen has not been previously reported, and equine-specific probes have not been available. We report the complete sequence and discuss the molecular characteristics of equine type II procollagen mRNA which was cloned from a cDNA library prepared from mRNA isolated from equine articular chondrocytes. The coding sequence (4257 bp) was 92.4% homologous to the cDNA of the human sequence, and the propeptide was 97% identical to the human sequence. We demonstrated that when equine chondrocytes are grown in phenotypically-maintained cultures, the expression of type II procollagen is linked to the age of the animal. Additionally, in cultures of young equine chondrocytes, IL1-beta and TNF-alpha both reduced the expression of type II procollagen mRNA in a dose responsive manner.
authors.affiliation: Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Richardson
authors.forename: G R
authors.initials: GR
authors.lastname: Dodge
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002358:Cartilage, Articular / Q000166:cytology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006736:Horses / Q000235:genetics*; D008969:Molecular Sequence Data; D011347:Procollagen / Q000235:genetics*
chemical_list: D011347:Procollagen