Identification of alpha 1-acid glycoprotein (orosomucoid) in human synovial fluid by capillary electrophoresis.
Capillary electrophoresis of human synovial fluid in a phosphate borate run buffer containing sodium dodecyl sulphate separates a hydrophilic glycoprotein, hyaluronan and a number of low-molecular-mass components. The hydrophilic glycoprotein is identified as alpha 1-acid glycoprotein (AGP), orosomucoid, by co-injection methods with human AGP and by reaction with neuraminidase which released N-acetylneuraminic acid. Finally, a sample of the glycoprotein was isolated by micropreparative capillary electrophoresis, examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis methods and shown to give a positive reaction with AGP antibodies. The peak due to AGP in the capillary electrophoresis is broad and gives evidence for the presence of glycoforms.
Rice
A
A
School of Biology and Biochemistry, Queen's University of Belfast, UK.
Grimshaw
J
J
Trocha-Grimshaw
J
J
McCarron
P
P
Wisdom
G B
GB
D000906:Antibodies / Q000276:immunology; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D019158:N-Acetylneuraminic Acid / Q000032:analysis / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism; D009961:Orosomucoid / Q000032:analysis*; D013053:Spectrophotometry; D013582:Synovial Fluid / Q000737:chemistry*
D000906:Antibodies; D006023:Glycoproteins; D009961:Orosomucoid; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid