title: Optimization of the performance of the polymerase chain reaction in silicon-based microstructures.
abstract: We have demonstrated the ability to perform real-time homogeneous, sequence specific detection of PCR products in silicon microstructures. Optimal design/ processing result in equivalent performance (yield and specificity) for high surface-to-volume silicon structures as compared to larger volume reactions in polypropylene tubes. Amplifications in volumes as small as 0.5 microl and thermal cycling times reduced as much as 5-fold from that of conventional systems have been demonstrated for the microstructures.
authors.affiliation: PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA.
authors.forename: T B
authors.initials: TB
authors.lastname: Taylor
authors.forename: E S
authors.initials: ES
authors.lastname: Winn-Deen
authors.forename: E
authors.initials: E
authors.lastname: Picozza
authors.forename: T M
authors.initials: TM
authors.lastname: Woudenberg
authors.forename: M
authors.initials: M
authors.lastname: Albin
mesh_terms: D000199:Actins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D012825:Silicon
chemical_list: D000199:Actins; D012825:Silicon