Topical application of low molecular weight heparin in a rabbit traumatic anastomosis model.
Low molecular weight heparins (LMWHs) are antithrombotic drugs composed of lower molecular weight components of heparin with an apparent molecular weight in the range of 4.0-8.0 KDA. These agents have been used clinically for several years. They have different mechanisms of action compared to heparin, a longer half-life and much higher bioavailability. Anticoagulant drugs such as heparin have been used topically in our previous studies to avoid bleeding complications observed with systemic administration. In this study, low molecular weight heparin (Certoparin, Sandoz) was topically administered in a rabbit ear arterial crush-avulsion thrombosis model and compared with heparin. The animals were divided into three groups: LMWH, heparin and saline control groups. In the LMWH group, the patency rate was 71% (10 of 14) at both 1 and 7 days. The patency rate in the heparin group was 95% (19 of 20) at 24 hrs and 80% (16 of 20) at 7 days. In the saline control group, the vessel patency rate was 17% at 24 hrs and 13% at 7 days. Clotting times such as ACT, PT and APTT performed on samples drawn one hour after drug administration were within the normal ranges for both the control and the treatment groups. The results suggest that topical administration of LMWH prevents the occurrence of thrombosis at the traumatic anastomosis site to a similar degree as heparin.
Fu
K
K
Department of Surgery, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153, USA.
Izquierdo
R
R
Vandevender
D
D
Warpeha
R L
RL
Wolf
H
H
Fareed
J
J
D000287:Administration, Topical; D000714:Anastomosis, Surgical; D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage*; D001158:Arteries / Q000293:injuries / Q000601:surgery; D004195:Disease Models, Animal; D004423:Ear / Q000098:blood supply; D006493:Heparin / Q000008:administration & dosage; D006495:Heparin, Low-Molecular-Weight / Q000008:administration & dosage*; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D013927:Thrombosis / Q000517:prevention & control*
D000925:Anticoagulants; D006495:Heparin, Low-Molecular-Weight; D006493:Heparin