Results of a combined dexamethasone suppression/thyrotropin-releasing hormone stimulation test in healthy horses and horses suspected to have a pars intermedia pituitary adenoma.
OBJECTIVE
To evaluate results of a combined dexamethasone suppression/thyrotropin-releasing hormone (TRH) stimulation test in horses suspected clinically to have a pars intermedia pituitary adenoma (PIPA).


METHODS
Case-control study.


METHODS
7 healthy adult horses and 5 horses suspected to have a PIPA.


METHODS
A baseline blood sample was collected, and dexamethasone (40 micrograms/kg [18 micrograms/lb] of body weight, IV) was administered; a second blood sample was collected 3 hours later, and TRH (1.1 mg, IV) was administered; serial blood samples were collected 15, 30, 45, 60, and 90 minutes and 21 hours after TRH administration (24 hours after dexamethasone injection). Cortisol concentration was determined for all blood samples.


RESULTS
Baseline cortisol concentration was significantly lower in horses suspected to have a PIPA than in healthy horses. Cortisol concentration was suppressed by dexamethasone in both groups; however, after TRH administration, cortisol concentration returned to baseline values in horses suspected to have a PIPA, but not in healthy horses. Concentration was still less than the baseline value 24 hours after dexamethasone administration in healthy horses.


CONCLUSIONS
The combined dexamethasone suppression/TRH stimulation test may be a useful diagnostic test in horses suspected to have a PIPA. For clinical application, collection of a blood sample 30 minutes after TRH administration is recommended.
Eiler
H
H
Department of Animal Science, College of Veterinary Medicine, University of Tennessee, Knoxville 37996-4500, USA.
Oliver
J W
JW
Andrews
F M
FM
Fecteau
K A
KA
Green
E M
EM
McCracken
M
M
D000236:Adenoma / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*; D000818:Animals; D016022:Case-Control Studies; D003907:Dexamethasone; D005260:Female; D005938:Glucocorticoids; D006734:Horse Diseases / Q000175:diagnosis / Q000503:physiopathology*; D006736:Horses / Q000502:physiology*; D006854:Hydrocortisone / Q000097:blood*; D010902:Pituitary Gland / Q000503:physiopathology; D010911:Pituitary Neoplasms / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000187:drug effects; D013973:Thyrotropin-Releasing Hormone
D005938:Glucocorticoids; D018025:Receptors, Thyrotropin-Releasing Hormone; D013973:Thyrotropin-Releasing Hormone; D003907:Dexamethasone; D006854:Hydrocortisone