Centromeric and noncentromeric ADE2-selectable fragmentation vectors for yeast artificial chromosomes in AB1380.
We have constructed a set of fragmentation vectors for the truncation of either the centromeric or the noncentromeric end of YACs containing a human DNA insert. These vectors carry ADE2 or HIS5 as the selectable marker, enabling direct use in AB1380, the host strain of most publicly available YAC libraries. Centromeric fragmentation vectors for AB1380 have not been reported previously; the noncentromeric vectors show high frequencies of fragmentation.
Heus
J J
JJ
Department of Human Genetics, Leiden University, The Netherlands.
de Winther
M P
MP
van de Vosse
E
E
van Ommen
G J
GJ
den Dunnen
J T
JT
D002262:Carboxy-Lyases / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D053938:DNA Fragmentation; D005819:Genetic Markers; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016254:Mutagenesis, Insertional; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D005819:Genetic Markers; D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase