Effect of hydrostatic pressure of a mutant of Saccharomyces cerevisiae deleted in the trehalose-6-phosphate synthase gene.
Mutants Saccharomyces cerevisiae deleted on the trehalose-6-phosphate synthase gene (tps1) and their parental wild-type cells were submitted to hydrostatic pressure in the range of 0-200 MPa. Experimental evidence showed that viability for both strains decreased with increasing pressure and that tps1 mutants, unable to accumulate trehalose, were more sensitive to hydrostatic pressure than the wild-type cells. Additionally, both tps1 and wild-type cells in the stationary phase, when there is an accumulation of endogenous trehalose, were more resistant to pressure than proliferating cells. Under these conditions, mutant cells were also more sensitive to pressure treatment than the wild type. The present work also showed that mild pressure pretreatment did not induce hydrostatic pressure resistance (barotolerance) in yeast cells.
Fernandes
P M
PM
Departamento de Bioquimica, Instituto de Quimica, Universidade Federal do Rio de Janeiro, Brazil.
Panek
A D
AD
Kurtenbach
E
E
D017353:Gene Deletion; D005964:Glucosyltransferases / Q000235:genetics*; D006874:Hydrostatic Pressure; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D014199:Trehalose / Q000502:physiology*
D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase