Determination of cocaine and its metabolites in serum microsamples by high-performance liquid chromatography and its application to pharmacokinetics in rats.
A single-solvent extraction step high-performance liquid chromatographic method is described for quantitating cocaine and its three metabolites in rat serum microsamples (50 microl). The separation used a 2.1-mm I.D. reversed-phase Brownlee C18 column with an isocratic mobile phase consisting of methanol-acetonitrile-25.8 mM sodium acetate buffer, pH 2.2, containing 1.29-10(-4) M tetrabutylammonium phosphate (12.5:10:77.5, v/v/v). The detection limit was 2.5 ng/ml for all the compounds using an ultraviolet detector operated at 235 nm. The method was used to study the pharmacokinetics of cocaine after an intravenous (i.v.) bolus dose (4 mg/kg).
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Department of Psychology, Busch Campus, Rutgers University, New Brunswick, NJ 08903, USA.
Zhang
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J
Lau
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CE
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D009294:Narcotics / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D009294:Narcotics; C008671:norcocaine; C005618:benzoylecgonine; D003042:Cocaine; C012209:benzoylnorecgonine