Additional copies of the mitochondrial Ef-Tu and aspartyl-tRNA synthetase genes can compensate for a mutation affecting the maturation of the mitochondrial tRNAAsp.
In an attempt to identify new nuclear genes involved in the synthesis and processing of mitochondrial tRNAs, we utilized a multicopy nuclear library to suppress the heat-sensitive phenotype of a Saccharomyces cerevisiae mitochondrial mutant strain. This strain (Ts 932) is defective in the 3'-end processing of the mitochondrial tRNAAsp transcript. The nuclear genes coding for the mitochondrial elongation factor Tuf M and for the mitochondrial aspartyl-tRNA synthetase have been found to restore the temperature-resistant phenotype and to correct the RNA processing defect. Suppression was effective even when the genes were present on a centromeric plasmid.
Rinaldi
T
T
Pasteur Institute-Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome I, I-00185 Rome, Italy.
Lande
R
R
Bolotin-Fukuhara
M
M
Frontali
L
L
D001226:Aspartate-tRNA Ligase / Q000235:genetics*; D015152:Blotting, Northern; D002455:Cell Division / Q000235:genetics; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal; D016296:Mutagenesis; D009154:Mutation; D010088:Oxidoreductases; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional; D012349:RNA, Transfer, Asp / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature
D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D012349:RNA, Transfer, Asp; D029701:Saccharomyces cerevisiae Proteins; D010088:Oxidoreductases; C107530:SMM1 protein, S cerevisiae; D010444:Peptide Elongation Factor Tu; D001226:Aspartate-tRNA Ligase