title: Non-O157 Vero cytotoxin producing Escherichia coli: aetiological agents of diarrhoea in children in Dunedin, New Zealand.
abstract: Strains of Escherichia coli that produce Vero cytotoxin (VTEC) commonly cause diarrhoea, haemorrhagic colitis and haemolytic-uraemic syndrome in many northern hemisphere countries. In these countries, serotype O157:H7/H-predominates and has caused large food-borne outbreaks of infection. In contrast, few cases of infection with this serotype have been reported in New Zealand. Over a 3-month period, 484 stool specimens submitted to medical laboratories in Dunedin were screened for E. coli O157:H7/H-using sorbitol MacConkey agar, Y1 and Vero cell assays. Where possible, Vero cytotoxin production was confirmed by an ELISA test. Specimens from children aged 12 years or less were additionally screened for non-O157 VTEC. In the specimens of the children tested, O157:H7/H-VTEC was not isolated, but VTEC belonging to other serogroups were isolated from the children. Of interest was the detection of other species of Enterobacteriaceae, which produced a cytopathic effect on Vero cells. This study confirms the low incidence of infection with O157:H7/H- VTEC in New Zealand and suggests that non-O157 VTEC is a more important cause of diarrhoeal disease.
authors.affiliation: Department of Microbiology, University of Otago, Dunedin, New Zealand.
authors.forename: H J
authors.initials: HJ
authors.lastname: Brooks
authors.forename: K A
authors.initials: KA
authors.lastname: Bettelheim
authors.forename: B
authors.initials: B
authors.lastname: Todd
authors.forename: M D
authors.initials: MD
authors.lastname: Holdaway
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002648:Child; D002522:Chlorocebus aethiops; D003967:Diarrhea / Q000209:etiology* / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000209:etiology* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D006801:Humans; D009520:New Zealand / Q000453:epidemiology; D012703:Serotyping; D022622:Shiga Toxin 1; D014709:Vero Cells
chemical_list: D001427:Bacterial Toxins; D022622:Shiga Toxin 1