Structure and organization of the Bombyx mori sericin 1 gene and of the sericins 1 deduced from the sequence of the Ser 1B cDNA.
The sericin 1 primary transcript of the silkworm Bombyx mori is differentially spliced via a tissue- and developmentally-regulated process. From a middle silk gland cDNA library, we have elucidated the sequence of one of the four mRNAs, the 4.0 kb Ser1B mRNA. Determination of alternative or constitutive exons and intron-exon boundaries allowed us to establish the nine exon-eight intron structure of the Ser1 gene. From these and previous data, it was possible to deduce the sequence of the sericins 1 and to predict the secondary structure and physiochemical properties of the different regions of the proteins.
Garel
A
A
Centre de Génétique Moléculaire et Cellulaire, U.M.R., Université C. Bernard, Lyon I, Villeurbanne, France.
Deleage
G
G
Prudhomme
J C
JC
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D018076:DNA, Complementary; D005091:Exons; D017344:Genes, Insect; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry / Q000235:genetics*; D047030:Sericins
D018076:DNA, Complementary; D010456:Peptides, Cyclic; D047030:Sericins