RAP74 induces promoter contacts by RNA polymerase II upstream and downstream of a DNA bend centered on the TATA box.
RAP74, the large subunit of transcription factor IIF, associates with a preinitiation complex containing RNA polymerase II (pol II) and other general initiation factors. We have mapped the location of RAP74 in close proximity to promoter DNA at similar distances both upstream and downstream of a DNA bend centered on the TATA box. Binding of RAP74 induces a conformational change that affects the position of pol II relative to that of the DNA. This reorganization of the preinitiation complex minimally requires the N-terminal region of RAP74 containing both its RAP30-binding domain and another region necessary for accurate transcription in vitro. We propose a role for RAP74 in controlling the topological organization of the pol II preinitiation complex.
Forget
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Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Québec, PQ Canada J1K 2R1.
Robert
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Grondin
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Burton
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ZF
Greenblatt
J
J
Coulombe
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B
D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012319:RNA Polymerase II / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016385:TATA Box / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
D011994:Recombinant Proteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D004247:DNA; D012319:RNA Polymerase II; C056604:transcription factor TFIIF