Cloning and bacterial expression of a gene encoding ribosome-inactivating proteins, karasurin-A and karasurin-C, from Trichosanthes kirilowii var. japonica.
A genomic DNA clone of karasurin was isolated using the polymerase chain reaction from Trichosanthes kirilowii var. japonica (Cucurbitaceae). The amino acid sequence deduced from the nucleotide sequence was consistent with previously reported sequences of karasurin-A and karasurin-C except for a putative signal peptide and extra amino acids at the C-terminus, neither of which is present in the natural protein. Recombinant karasurin was synthesized in Escherichia coli, in which the cloned karasurin gene was expressed under the control of the trc promoter.
Mizukami
H
H
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Iida
K
K
Kondo
T
T
Ogihara
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D010940:Plant Proteins; D011994:Recombinant Proteins; C069870:karasurin protein, Trichosanthes kirilowii; D009699:N-Glycosyl Hydrolases