Dr fimbriae operon of uropathogenic Escherichia coli mediate microtubule-dependent invasion to the HeLa epithelial cell line.
Escherichia coli Dr adhesin and decay-accelerating factor (DAF) receptor-mediated interaction was proposed as the mechanism of ascending urinary tract infection (UTI) and chronic interstitial nephritis. This report provides novel evidence for Dr fimbriae operon-mediated invasive capacity of Dr+ E. coli. Insertional mutants draE, draC, and draB, and adherent draD and UV-inactivated BN406 were unable to enter HeLa cells. Complementation of the dra mutation restored invasiveness. Internalization was inhibited by anti-Dr fimbriae IgG (100%), anti-SCR-3 domain of DAF (75%), and nocodazole (95%). Increased receptor-ligand density occurred at the site of internalization. Internalized Dr+ E. coli did not significantly multiply in the HeLa cell line. Accordingly, the dra operon and DAF were required for microtubule-dependent internalization of E. coli to HeLa cells. The relatively low invasion and multiplication rates of Dr+ E. coli may hypothetically contribute to the postattachment steps of ascending UTI and chronic renal infection.
Goluszko
P
P
Department of Obstetrics & Gynecology, University of Texas Medical Branch, Galveston 77555-1062, USA.
Popov
V
V
Selvarangan
R
R
Nowicki
S
S
Pham
T
T
Nowicki
B J
BJ
D018830:Adhesins, Escherichia coli / Q000032:analysis / Q000235:genetics*; D001422:Bacterial Adhesion; D018958:CD55 Antigens / Q000032:analysis / Q000502:physiology; D015638:Cytochalasin D / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008870:Microtubules / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D009876:Operon; D014552:Urinary Tract Infections / Q000382:microbiology*
D018830:Adhesins, Escherichia coli; D018958:CD55 Antigens; D015638:Cytochalasin D; D015739:Nocodazole