[The effect of angelica polysaccharide on proliferation and differentiation of hematopoietic progenitor cell].
OBJECTIVE
To study the effect of angelica polysaccharide (AP) on proliferation and differentiation of hematopoietic progenitor cells for clarifying the hematonic mechanism of angelica sinensis.


METHODS
The techniques of culture of hematopoietic progenitor cell and hematopoietic growth factor (HGF) assay were used.


RESULTS
AP could obviously promote the proliferation and differentiation of BFU-E, CFU-E, CFU-GM and CFU-MK in healthy and aniemic mice. The culture media of splenocyte, macrophage, fibroblast and skeletal muscle treated with AP had much stronger stimulating effects on hematopoietic progenitor cells.


CONCLUSIONS
AP may enhance hematopoiesis by stimulating directly and/or indirectly macrophages, fibroblasts, lymphocytes in hematopoietic inductive microenvironment and muscle tissue to secrete some HGF (Epo, GM-CSF, IL, and MK-CSF). This is one of the biological mechanisms for hematonic effect of angelica sinensis.
Wang
Y
Y
Department of Histology and Embryology, Chongqing University of Medical Sciences.
Zhu
B
B
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D006397:Hematinics / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D008815:Mice, Inbred Strains; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D006397:Hematinics; D011134:Polysaccharides