Co-metabolism and microbial growth in the biodegradation of alkylbenzenesulphonates.
Two organisms, CCMI507 and CCMI852, degrading undecylbenzenesulphonate (LAS) by the ortho- and meta-cleavage pathways were studied in cultures where glucose was used as carbon and energy source. CCMI507 (ortho-pathway) started the degradation of LAS at the beginning of the culture development in parallel with glucose utilization. The degradation followed a steady profile of degradation until 77% of LAS was degraded in the culture containing initially 5 mg l-1 of the compound and 81% in the cultures containing initially 10 and 20 mg l-1 of LAS, after 72 h fermentation. The organism CCMI852 (meta-pathway) started degrading the compound only after 20 h, when 75% of glucose was spent and well within the stationary-phase. After 72 h fermentation the level of degradation by CCMI852 varied from 70% (5 mg l-1 of LAS) to around 75% (10 and 20 mg l-1 of LAS).
Marques
M L
ML
Instituto Nacional de Engenharia e Tecnologia Industrial, IBQTA-Laboratório de Microbiologia Industrial, Azinhaga dos Lameiros à Estrada do Paço do Lumiar, Lisboa, Portugal.
Silva
J
J
Roseiro
J C
JC
D001557:Benzenesulfonates / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D049308:Dioxygenases; D005285:Fermentation; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D010105:Oxygenases / Q000378:metabolism; D016958:Pseudomonas putida / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism
D001557:Benzenesulfonates; D013501:Surface-Active Agents; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D005947:Glucose