A 900 bp genomic region from the mouse dystrophin promoter directs lacZ reporter expression only to the right heart of transgenic mice.
In order to study the regulatory mechanism of developmental and tissue-specific expression of the muscle type dystrophin gene in mice, transgenic mice were generated carrying the 900 bp genomic fragment derived from the muscle type dystrophin promoter region fused to the bacterial lacZ gene. Six independent transgenic mouse lines showed specific reporter gene expression in the right heart, but not in skeletal or smooth muscle. The reporter gene expression was first detected in the presumptive right ventricle of the embryos at 8.5 days post coitum and the expression continued only in the right ventricle throughout the development and at the adult stage. The results indicate that the 900 bp genomic fragment contains the regulatory element required for expression of dystrophin only in the right heart, suggesting that distinct elements are responsible for the expression in the left and right compartments of the heart, and/or in skeletal and smooth muscle cells. Based on these findings, the relationship between defects in muscle type promoter and the diseases caused by abnormal dystrophin expression is discussed.
Kimura
S
S
Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 862, Japan.
Abe
K
K
Suzuki
M
M
Ogawa
M
M
Yoshioka
K
K
Kaname
T
T
Miike
T
T
Yamamura
K
K
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D016189:Dystrophin / Q000235:genetics* / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D016678:Genome; D006321:Heart / Q000196:embryology / Q000254:growth & development*; D006352:Heart Ventricles / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D007763:Lac Operon; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D011401:Promoter Regions, Genetic
D016189:Dystrophin; D004247:DNA