Rapid cytocidal and cytostatic chemosensitivity test by measuring total amount of mRNA.
Chemosensitivity of vinblastin, cisplatin, and mitomycin C were assessed in four different human cancer cell lines (U937, HL-60, CaR-1, and HepG2) by MTT (3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) assay and the measurement of total cytosolic poly(A) + mRNA. Results of 12 h mRNA assay with 10 x peak plasma concentration (PPC) were significantly correlated with that of standard 3 days MTT assay with 1 x PPC. Furthermore, mRNA assay was changed more significantly than MTT assay under cytostatic condition. Because of its minimum culture requirement (12 h) and broad spectrum (cytocidal and cytostatic), mRNA assay will become a useful tool for chemosensitivity test.
Miura
Y
Y
Department of Pathology, University of California, Irvine, USA.
deFries
R
R
Shimada
H
H
Mitsuhashi
M
M
D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis*; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue