Loss of thymidine kinase activity due to a base deletion in a candidate vaccine strain of bovine herpesvirus 2.
The nucleotide (nt) sequence of the thymidine kinase (TK) gene (a 918 nt long coding region) of two TK-deficient (TK) strains of bovine herpesvirus 2 (BHV-2) was determined. The candidate vaccine strain C290BU5, which was no longer able to cause disease, was found to have an A deletion after nt 887 of the TK gene with a predicted change of His 296 to Pro, altering the last 10 amino acids (aa) and extending the gene by another 34 aa. The strain which still caused disease, C290BU3, had a T insertion after nt 16 causing a predicted chain termination after only 16 aa.
Gaut
R
R
School of Microbiology, La Trobe University, Bundoora, Victoria, Australia.
May
J T
JT
D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D001911:Herpesvirus 2, Bovine / Q000201:enzymology* / Q000235:genetics; D017354:Point Mutation; D017384:Sequence Deletion; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics*
D004279:DNA, Viral; D015678:Viral Structural Proteins; D014765:Viral Vaccines; D013937:Thymidine Kinase