Crystallization and preliminary X-ray analysis of the single-headed and double-headed motor protein kinesin.
Crystals of the single-headed and double-headed kinesin motor domains of Rattus norvegicus have been grown by vapor diffusion using ammonium sulfate as the precipitant. Both crystal systems belong to the orthorhombic space group P2(1)2(1)2(1). Double-headed kinesin crystallized with unit cell constants of a = 72.2 A, b = 91.9 A, and c = 141.7 A, and so far the best crystals diffracted to a maximum resolution of 2.7 A. Using ammonium sulfate single-headed kinesin crystallized in two different crystal forms with cell constants of a = 73.1 A, b = 73.2 A, c = 84.0 A and a = 73.4 A, b = 74.1 A, c = 74.7 A, respectively. They were found to diffract to 2.1 A resolution. Crystals of monomeric kinesin were also obtained with lithium sulfate as precipitant. They have cell constants of a = 71.6 A, b = 73.7 A, and c = 74.1 A and diffract up to 1.7 A resolution.
Kozielski
F
F
Max-Planck-Unit for Structural Molecular Biology, Hamburg, Germany. frank@unisgil.desy.de
Schönbrunn
E
E
Sack
S
S
Müller
J
J
Brady
S T
ST
Mandelkow
E
E
D000818:Animals; D001923:Brain Chemistry; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D016547:Kinesins / Q000737:chemistry*; D018020:Lithium Compounds; D008970:Molecular Weight; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D013431:Sulfates; D014404:Tubulin / Q000302:isolation & purification / Q000378:metabolism
D018020:Lithium Compounds; D011994:Recombinant Proteins; D013431:Sulfates; D014404:Tubulin; C054097:lithium sulfate; D016547:Kinesins