[Mutagenic effect of nitrosodimethylurea in male mice. Induction of dominant lethal mutations and chromosome aberrations in germ cells; genotype influence on the clastogenic effect in bone marrow cells].
The ability of dimethylnitrosourea (DMNU) to induce dominant lethal mutations in germ cells and chromosome aberrations in bone marrow cells of male CBAB6F1 mice was studied. At a mutagen dose of 200 mg/kg, mortality was 19% in the second week after treatment (being 5% in the control) and the frequency of bone marrow cells containing chromosome aberrations was 19.6% 24 h after treatment. DMNU induces dominant lethals in postmeiotic cells and in spermatogonia; the effects in spermatozoids and spermatogonia are equal. Chromosome aberrations in spermatocytes induced by DMNU are not realized as dominant lethals. The sensitivity of mouse strains to the clastogenic effect of DMNU ranged in an order similar to that observed in experiments with thioTEPA. The most sensitive was the TPS strain (29.2 +/- 4.6% of cells with chromosome aberrations), the most resistant-the CBA/Lac strain (9.5 +/- 2.9%). DMNU exhibited a relatively poor clastogenic activity; the effect in bone marrow cells was higher than that in male germ cells.
Malashenko
A M
AM
Beskova
T B
TB
Vasil'eva
S V
SV
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002869:Chromosome Aberrations; D005799:Genes, Dominant; D005804:Genes, Lethal; D005838:Genotype; D008297:Male; D008770:Methylnitrosourea / Q000031:analogs & derivatives* / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D013093:Spermatogonia / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*
D009153:Mutagens; C001049:N,N'-dimethylnitrosourea; D008770:Methylnitrosourea