Recombinant env-gp85 of HPRS-103 (subgroup J) avian leukosis virus: antigenic characteristics and usefulness as a diagnostic reagent.
We describe the construction of a recombinant baculovirus containing the cloned DNA encoding the gp85 envelope glycoprotein of HPRS-103 (subgroup J) avian leukosis virus fused to the carboxy-terminus of the affinity tag glutathione-S-transferase. The fusion protein was efficiently secreted into the supernatant medium of the infected insect cell culture and could be purified in a single step using immobilized glutathione. An enzyme-linked immunosorbent assay using the recombinant protein was found to be specific and sensitive for detection of HPRS-103 virus-specific antibodies in the sera of infected birds.
Venugopal
K
K
Institute for Animal Health, Compton, Nr. Newbury, Berkshire, United Kingdom.
Howes
K
K
Barron
G S
GS
Payne
L N
LN
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D001353:Avian Leukosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D001354:Avian Leukosis Virus / Q000145:classification / Q000302:isolation & purification*; D002460:Cell Line; D002645:Chickens; D002846:Chromatography, Affinity; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D018411:Spodoptera; D014162:Transfection; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D000956:Antigens, Viral; D017931:DNA Primers; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C107403:glycoprotein 85, avian leukosis virus