Synthesis, characterization and biocompatibility of PEGA resins.
Three types of beaded polyethylene glycol polyacrylamide copolymers (PEGA) with a high content of polyethylene glycol (PEG) were synthesized by inverse suspension polymerization and characterized for peptide synthesis and with respect to their physical properties. Several peptides of high purity have been synthesized on the resin. The properties which were determined were loading of amino groups, swelling, bead size distribution, porosity, flexibility and compatibility with active biomolecules. A loading of 0.35 mmol/g has been obtained and the swelling was excellent in solvents of various polarities ranging from water to dichloromethane. The 13C-NMR T1-relaxation times of a resin containing a peptide were determined in DMSO-d6 and the resin was found to exhibit a behavior similar to the components in free solution.
Auzanneau
F I
FI
Department of Chemistry, Carlsberg Laboratory, Valby-Copenhagen, Denmark.
Meldal
M
M
Bock
K
K
D000180:Acrylic Resins / Q000138:chemical synthesis* / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D001978:Bromphenol Blue; D002850:Chromatography, Gel; D004800:Enzymes, Immobilized / Q000378:metabolism; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D015394:Molecular Structure; D010316:Particle Size; D010455:Peptides / Q000138:chemical synthesis*; D010539:Permeability; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D012996:Solutions; D000099166:Polyacrylamides
D000180:Acrylic Resins; D001672:Biocompatible Materials; D001978:Bromphenol Blue; D004800:Enzymes, Immobilized; D010455:Peptides; D011092:Polyethylene Glycols; D012996:Solutions; D000099166:Polyacrylamides