Molecular cloning and expression of a bovine endothelial inward rectifier potassium channel.
A 5.1 kb cDNA encoding an inward rectifier K+ channel (BIK) was isolated from a bovine aortic endothelial cell library. The cDNA codes for a 427-amino-acid protein with two putative transmembrane regions. Sequence analysis reveals that BIK is a member of the Kir2.1 family of inward rectifier K+ channels. Expression in Xenopus oocytes showed that BIK is a K+-specific strong inward rectifier channel that is sensitive to extracellular Ba2+, Cs+, and a variety of anti-arrhythmic agents. Northern analysis revealed that endothelial cells express a 5.5 kb BIK mRNA that is sensitive to shear stress.
Forsyth
S E
SE
Department of Bioengineering, University of California at San Diego, La Jolla 92093, USA.
Hoger
A
A
Hoger
J H
JH
D000595:Amino Acid Sequence; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D001011:Aorta; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000378:metabolism* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D015221:Potassium Channels / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D024661:Potassium Channels, Inwardly Rectifying; D012212:Rheology
D000889:Anti-Arrhythmia Agents; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying