99mTc-labelled diphytanoylphosphatidylcholine liposomes: in vitro and in vivo studies.
The uniquely structured diphytanoylphosphatidylcholine (DphPC) forms liposomes more stable than conventional straight chain phospholipids. In this study DphPC and pegylated DphPC (DphPC-PEG) liposomes were radiolabelled and evaluated in vitro and in vivo. 99mTc-DphPC liposomes were found to be nontoxic to human white blood cells in vitro. In addition 99mTc labelled DphPC-PEG liposomes were evaluated as a nonspecific infection imaging agent in a mouse model. Infection sites were imaged within 30 min postinjection, and the radiopharmaceutical exhibited a remarkable in vivo stability. As their biodistribution and pharmacokinetic patterns can be size-modulated, DphPC-based lipsomes are excellent candidates for diagnostic and therapeutic applications.
Andreopoulos
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Department of Nuclear Medicine, University of Texas, M.D. Anderson Cancer Center, Houston 77030, USA.
Kasi
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LP
D000818:Animals; D004337:Drug Carriers; D004355:Drug Stability; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D008081:Liposomes; D008297:Male; D051379:Mice; D010713:Phosphatidylcholines / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D011092:Polyethylene Glycols; D013667:Technetium; D014018:Tissue Distribution
D004337:Drug Carriers; D008081:Liposomes; D010713:Phosphatidylcholines; C021016:1,2-diphytanoylphosphatidylcholine; D011092:Polyethylene Glycols; D013667:Technetium