title: GAP1 activity is dependent on cAMP in Saccharomyces cerevisiae.
abstract: General amino acid permease (GAP1) activity was evaluated in adenylate cyclase-deficient Saccharomyces cerevisiae to determine the effect of cAMP levels on GAP1 activity. Lowering cAMP concentrations in the culture media led to a decrease in the initial rates of L-citrulline uptake. Kinetics of the amino acid transport system showed a partial loss of transport capacity, with no apparent modifications in permease affinity.
authors.affiliation: Departamento de Bioquímica, Facultad de Medicina, Universidad de Buenos Aires-CONICET, Argentina.
authors.forename: A A
authors.initials: AA
authors.lastname: Amitrano
authors.forename: D A
authors.initials: DA
authors.lastname: Saenz
authors.forename: E H
authors.initials: EH
authors.lastname: Ramos
mesh_terms: D026905:Amino Acid Transport Systems; D001692:Biological Transport / Q000187:drug effects; D002956:Citrulline / Q000493:pharmacokinetics; D000242:Cyclic AMP / Q000494:pharmacology*; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D026905:Amino Acid Transport Systems; D026901:Membrane Transport Proteins; D002956:Citrulline; D000242:Cyclic AMP