Favorable effect of hemodialysis on decreased serum antioxidant activity in hemodialysis patients demonstrated by electron spin resonance.
There is considerable evidence that uremic patients are in a highly peroxidative state. The purpose of this study was to investigate the serum antioxidant activity that may regulate, or represent, the redox state in vivo. Serum from pre- and posthemodialysis patients and from healthy control subjects was added to a system generating the hydroxyl radical, and then the signal intensities of reactive oxygen species were measured by electron spin resonance and spin-trapping technique. The electron spin resonance signals of the reaction mixture containing prehemodialysis sera were significantly stronger than those of the reaction mixture containing healthy sera (P < 0.001, n = 19), and there was no significant difference in the signals between the reaction mixture containing posthemodialysis and healthy sera. These findings demonstrated that serum antioxidant activity in hemodialysis patients is significantly decreased, and this pathological condition is improved by hemodialysis treatment.
Nagase
S
S
Department of Internal Medicine, University of Tsukuba, Ibaraki, Japan.
Aoyagi
K
K
Hirayama
A
A
Gotoh
M
M
Ueda
A
A
Tomida
C
C
Kamezaki
T
T
Nagai
Y
Y
Kikuchi
H
H
Koyama
A
A
D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D006801:Humans; D017665:Hydroxyl Radical / Q000097:blood; D008297:Male; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000378:metabolism; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood* / Q000628:therapy*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; D002374:Catalase