Structure of the O-polysaccharide from the LPS of a Hafnia alvei strain isolated from a patient with suspect yersinosis.
The carbohydrate backbone of the Hafnia alvei strain Y166/91 lipopolysaccharide (LPS) was isolated by mild acid hydrolysis followed by gel permeation chromatography and studied by NMR spectroscopy and methylation analysis. Treatment with periodate and hypoiodite gave a modified polysaccharide which was also characterised. It was concluded that the polysaccharide has the following structure with two distinct regions, which are connected. The chain length parameters m and n were not determined but the ratio m/n is approximately 2. [-->3)-beta-D-Galp-1(1-->3)-alpha-D-Galp-(1-->]m[-->3)-alpha-D-Galp-(1-- >3)- beta-D-Galf-(1-->]n From the present data it is not possible to determine whether it is the Galp-Galp chain or the Galp-Galf chain that is connected to the core. The structure found here is identical to that suggested for the O-specific polysaccharide chain of Klebsiella pneumoniae O1K2 (NCTC 5055) LPS [O. Kol, J.-M. Wieruszeski, G. Strecker, J. Montreuil, and B. Fournet, Carbohydr. Res., 217 (1991) 117-125; O. Kol, J.-M. Wieruszeski, G. Strecker, B. Fournet, R. Zalisz, and P. Smets, Carbohydr. Res., 236 (1992) 339-344].
Karlsson
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Clinical Research Centre, Karolinska Institute, Huddinge Hospital, Sweden.
Jansson
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Wollin
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D002240:Carbohydrate Sequence; D006090:Gram-Negative Bacteria / Q000737:chemistry*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*
D008070:Lipopolysaccharides; D011134:Polysaccharides