[Effect of birchdust on viability of alveolar macrophages and superoxide produced by alveolar macrophages in vitro].
Rabbit alveolar macrophages (AM) in vitro were used as a model in this wooddust toxicological experiment to study the effect of birchdust with different concentrations and exposure durations on the viability of AM and superoxide produced by AM. The results showed that the viability rates in three birchdust groups (400, 800, 1600 micrograms/ml) gradually decreased when the concentration at all the designed exposure durations (12, 18, 24 h) increased except the rates for the 6-hour period. On the contrary, the contents of superoxide (O2-.) at the first two durations (6, 12 h) increased rapidly and reached their highest level at the 18 hours. Moreover, the rapid increase of O2-. preceded the declination of viability rates, suggesting that birchdust toxicity in AM in vitro be probably related to dust-induced O2-. produced by AM.
Yang
J
J
Department of Occupational Health, School of Public Health, Chengdu.
Wang
M
M
Lü
X
X
Ke
F
F
D000818:Animals; D002470:Cell Survival; D004391:Dust / Q000009:adverse effects*; D005609:Free Radicals; D016676:Macrophages, Alveolar / Q000502:physiology*; D011817:Rabbits; D013481:Superoxides / Q000378:metabolism*; D014934:Wood
D004391:Dust; D005609:Free Radicals; D013481:Superoxides