title: Solution conformations of a peptide containing the cytoplasmic domain sequence of the beta amyloid precursor protein.
abstract: The cytoplasmic domain of the beta amyloid precursor protein (betaAPP) may play a role in cellular events that lead to the secretion of the Abeta peptide, the major constituent of amyloid plaques found in the brains of individuals affected by Alzheimer's disease, by interacting with cellular factors involved in betaAPP function or processing. In order to elucidate the structural basis of cytoplasmic domain activity, the conformations adopted in solution by a peptide containing the 47-residue C-terminal sequence of betaAPP have been investigated by NMR and CD spectroscopy. The peptide does not have a stable tertiary structure, but local regions of the polypeptide chain populate defined conformations. In particular, the amino acid sequences TPEE and NPTY form type I reverse turns. These structured regions correspond to sequences within the cytoplasmic domain implicated in the biological activity of betaAPP.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA.
authors.forename: C D
authors.initials: CD
authors.lastname: Kroenke
authors.forename: D
authors.initials: D
authors.lastname: Ziemnicka-Kotula
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Kotula
authors.forename: A G
authors.initials: AG
authors.lastname: Palmer
mesh_terms: D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012996:Solutions
chemical_list: D016564:Amyloid beta-Protein Precursor; D010446:Peptide Fragments; D012996:Solutions