Sodium ascorbate induces DNA single-strand breaks in human cells in vitro.
Incubation of human lymphocytes, neonatal fibroblasts, and Molt-4 cells (T-cell leukemia cell line) with sodium ascorbate for 1 h resulted in a concentration-dependent increase in DNA single-strand breaks as assayed by an alkaline microgel electrophoresis technique. Fibroblasts and Molt-4 cells were significantly more sensitive than lymphocytes to the induction of DNA single-strand breaks by 25, 50, and 100 microM concentrations of sodium ascorbate. Significant cell loss was observed in Molt-4, but not in lymphocyte and fibroblast cultures, after 4 h of incubation in 50 microM of sodium ascorbate, a concentration similar to the plasma level of ascorbic acid in humans.
Singh
N P
NP
Bioelectromagnetics Research Laboratory, College of Medicine, University of Washington, Seattle 98195, USA.
D001205:Ascorbic Acid / Q000737:chemistry*; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D004252:DNA Mutational Analysis / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000737:chemistry; D009152:Mutagenicity Tests / Q000379:methods*
D004247:DNA; D001205:Ascorbic Acid