Vitamin D receptor expression in chicken muscle tissue and cultured myoblasts.
Muscle has long been recognized as a target tissue for 1,25-dihydroxy-vitamin D3 (1,25[OH]2D3). Evidence of the presence of VDR is provided here, thus supporting the existence of a receptor-mediated mechanism of action of 1,25(OH)2D3. Vitamin D receptor (VDR) expression is evidenced by detection of VDR-mRNA, through reverse transcription and polymerase chain reaction (RT/PCR), in chicken muscle and muscle cells (myoblasts) as well as in a variety of tissues such as intestine, kidney, heart and brain. VDR presence is also demonstrated by Southern blot of PCR products with a specific VDR-cDNA probe and by immunocytochemistry carried out on myoblasts and cardiac myocytes. Localization of VDR is mainly nuclear and more faintly detected in the cytosol.
Zanello
S B
SB
Departamento de Biología, Bioquímica y Farmacia, Universidad Nacionaldel Sur, Bahía Blanca, Argentina.
Collins
E D
ED
Marinissen
M J
MJ
Norman
A W
AW
Boland
R L
RL
D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D002642:Chick Embryo; D009132:Muscles / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000378:metabolism*
D012333:RNA, Messenger; D018167:Receptors, Calcitriol