title: Intercellular adhesion molecule-1 on cultured human epithelial cell lines: influence of proinflammatory cytokines.
abstract: The expression of intercellular adhesion molecule-1 (CD54 or ICAM-1) on epithelial cells during acute or chronic inflammation may favor the interaction between epithelial cells and leukocytes expressing the natural ligands of ICAM-1, LFA-1 (CD11a/CD18), and Mac-1 (CD11b/CD18). We have evaluated in vitro the expression of ICAM-1 by a conjunctival (WK) and an intestinal (I407) human continuous epithelial cell line. Cells were cultured for 24 h in the presence or absence of IFN-gamma, TNF-alpha, IL-1 beta, IL-4, IL-6, IL-8, IL-10, and TGF-beta 1. Both epithelial cell lines showed a constitutive expression of ICAM-1. IFN-gamma at 500 U/ml and TNF-alpha at 200 ng/ml upregulated ICAM-1 expression; IL-1 beta at 100 pg/ml upregulated ICAM-1 on WK cells only. Cells cultured in the presence of both IFN-gamma and TNF-alpha exhibited a mean fluorescence intensity far greater than those cultured with IFN-gamma or TNF-alpha alone. I407 and WK cells were able to release soluble ICAM-1. IFN-gamma and TNF-alpha enhanced the release of sICAM-1. IL-4, IL-6, IL-8, IL-10, and TGF-beta 1 did not affect either ICAM-1 expression or sICAM-1 release. In conclusion, continuously cultured human epithelial cells may express ICAM-1 on their surface and release it in culture medium. These phenomena are upregulated by proinflammatory cytokines.
authors.affiliation: Dipartimento di Medicina Interna, University of Genoa, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Paolieri
authors.forename: M
authors.initials: M
authors.lastname: Battifora
authors.forename: A M
authors.initials: AM
authors.lastname: Riccio
authors.forename: G
authors.initials: G
authors.lastname: Pesce
authors.forename: G W
authors.initials: GW
authors.lastname: Canonica
authors.forename: M
authors.initials: M
authors.lastname: Bagnasco
mesh_terms: D002448:Cell Adhesion / Q000276:immunology; D002478:Cells, Cultured; D003228:Conjunctiva / Q000276:immunology*; D016207:Cytokines / Q000276:immunology*; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology* / Q000378:metabolism*; D007371:Interferon-gamma / Q000276:immunology; D007378:Interleukins / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D007378:Interleukins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma