Dissociated secretion of islet amyloid polypeptide and insulin in serum-free culture media conditioned by human pancreatic adenocarcinoma cell lines.
CONCLUSIONS
The cosecretion of insulin and islet amyloid polypeptide (IAPP) is altered when isolated rat pancreatic islets are incubated in culture media conditioned by human pancreatic cancer cells.


BACKGROUND
Pancreatic cancer is usually associated with impaired glucose tolerance. This study investigates the tumor-derived influence on beta-cell secretion of pancreatic islets.


METHODS
Four conditioned media were prepared from two human pancreatic cancer cell lines (Panc-1 and HPAF), a hamster pancreatic cancer cell line (PC-1), and a fibroblast cell line (Ag1523). Isolated rat pancreatic islets were incubated first in the conditioned media or nonconditioned control medium for 24 h, then in the same kind of media containing 100 microM carbamylcholine for 90 min. Insulin and IAPP secretion were measured by radioimmunoassay.


RESULTS
Islets in media conditioned by Panc-1 and HPAF cells demonstrated dissociation of insulin and IAPP secretion. During 24-h incubation, the dissociation was expressed as selectively decreased insulin secretion. With addition of 100 microM carbamylcholine, the dissociation was expressed as normal secretion of insulin and hypersecretion of IAPP. As a result, the IAPP/insulin molar ratios were increased in both groups during both time periods. The islets in PC-1 and Ag1523 media did not show any significant changes in insulin and IAPP secretion.
Wang
F
F
Arvid Wretlind Laboratory for Nutritional and Metabolic Research Department of Surgery, Karolinska Institute at Huddinge University Hospital, Sweden.
Larsson
J
J
Abdiu
A
A
Gasslander
T
T
Westermark
P
P
Adrian
T E
TE
Permert
J
J
D000230:Adenocarcinoma / Q000378:metabolism*; D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
D000682:Amyloid; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D007328:Insulin; D058228:Islet Amyloid Polypeptide