The role of human leukocyte antigen in susceptibility and clinical manifestations of sarcoidosis.
OBJECTIVE
To investigate the correlation of human leukocyte antigen (HLA) with susceptibility and clinical manifestations of sarcoidosis.


METHODS
Fifty-five patients with sarcoidosis and one hundred and six normal subjects were investigated. Genomic DNAs were purified using the proteinase K-phenol extraction method. DNA samples were amplified by the polymerase chain reaction (PCR) procedure using the DRB1 group specific primer pairs.


RESULTS
The gene frequency of HLA-DR5 increased significantly in patients with sarcoidosis (P < 0.01), and HLA-DR7 decreased (P < 0.05). Gene frequencies of HLA-DR9 and HLA-DR5 increased remarkably in male patients and in patients with stage I and stage IIa, respectively (both P < 0.05).


CONCLUSIONS
It is suggested that HLA-DR gene might contribute to the susceptibility as well as various clinical manifestations of sarcoidosis.
Xu
Z
Z
Department of Respiratory Medicine, Peking Union Medical College (PUMC) Hospital, Beijing.
Zhao
Y
Y
Zhu
X
X
Qiu
C
C
Luo
W
W
Zhu
Y
Y
D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004198:Disease Susceptibility; D005260:Female; D005787:Gene Frequency; D015805:HLA-DR5 Antigen / Q000235:genetics / Q000502:physiology*; D015809:HLA-DR7 Antigen / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017565:Sarcoidosis, Pulmonary / Q000235:genetics*
D015805:HLA-DR5 Antigen; D015809:HLA-DR7 Antigen