title: Synthetic peptide combinatorial libraries: a method for the identification of bioactive peptides against phytopathogenic fungi.
abstract: Synthetic combinatorial libraries were evaluated with an iterative process to identify a hexapeptide with broadspectrum activity against selected phytopathogenic fungi. A D-amino acid hexapeptide (FRLKFH) and pentapeptide (FRLHF) exhibited activity against Fusarium oxysporum f. sp. lycopersici, Rhizoctonia solani (anastomosis group 1), Ceratocystis fagacearum, and Pythium ultimum. The peptides showed no hemolytic or mutagenic activity. Fluorescent microscopy studies with a membrane impermeant dye indicated that fungal cytoplasmic membranes were compromised rapidly and that the nuclear membrane was also affected.
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station 77843-2132, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Reed
authors.forename: D L
authors.initials: DL
authors.lastname: Edwards
authors.forename: C F
authors.initials: CF
authors.lastname: Gonzalez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects / Q000472:pathogenicity; D001665:Binding Sites; D002417:Cattle; D004353:Drug Evaluation, Preclinical; D005670:Fusarium / Q000187:drug effects / Q000472:pathogenicity; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009153:Mutagens / Q000494:pharmacology; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D019151:Peptide Library; D010944:Plants / Q000382:microbiology; D011775:Pythium / Q000187:drug effects / Q000472:pathogenicity; D012232:Rhizoctonia / Q000187:drug effects / Q000472:pathogenicity
chemical_list: D000935:Antifungal Agents; D009153:Mutagens; D009842:Oligopeptides; D019151:Peptide Library