title: Identification of spin diffusion pathways in proteins by isotope-assisted NMR cross-relaxation network editing.
abstract: A new isotope-assisted cross-relaxation editing experiment, [1H-13C]DINE-NOESY[1H-15N]HSQC (DINE=Double INEPT Edited), is proposed. It is based on the selective inversion of CH/CH3 or CH2 protons in the middle of the mixing time. The experiment sorts out the spin diffusion paths according to the principal mediators, either the CH/CH3 or the CH2 protons. This is useful in the structure refinement process, as it enables proper alignment of the aliphatic protons in the vicinity of NH protons.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Graduate School, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
authors.forename: N
authors.initials: N
authors.lastname: Juranić
authors.forename: Z
authors.initials: Z
authors.lastname: Zolnai
authors.forename: S
authors.initials: S
authors.lastname: Macura
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D006801:Humans; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008962:Models, Theoretical; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D014452:Ubiquitins / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D011506:Proteins; D014452:Ubiquitins; D006859:Hydrogen