Interaction of rabbit C-reactive protein with phospholipid monolayers studied by microfluorescence film balance with an externally applied electric field.
C-reactive protein (CRP) is one of the most characteristic acute-phase proteins in humans and many other animals. It binds to phosphorylcholine in a calcium-dependent manner. In addition, CRP activates the complement systems via the classical pathway. The interaction between rabbit CRP (rCRP) and model biological membrane is studied using dimyristoylphosphatidylethanolamine and dipalmitoylphosphatidylcholine monolayers. Observations with fluorescence microscopy indicate that rCRP is more likely to be incorporated in the liquid phase of monolayers. Such incorporation does not depend on the presence of calcium and is not inhibited by phosphocholine. The area occupied by the protein when incorporated into the monolayer was estimated. The dipole moment density of the protein crossing the air/water interface was measured by applying an external electric field. Our results indicate that calcium binding leads to a conformational change in CPR, which might modify the orientation of CRP in the monolayer. In addition, a negative charge or negative difference in dipole moment density facilitates the incorporation of CPR into the monolayer.
Mi
L Z
LZ
Department of Biological Sciences and Blotechnology, Tsinghua University, Beijing, People's Republic of China.
Wang
H W
HW
Sui
S F
SF
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002097:C-Reactive Protein / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D012235:Rhodamines
D005456:Fluorescent Dyes; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D012235:Rhodamines; D015060:1,2-Dipalmitoylphosphatidylcholine; D002097:C-Reactive Protein; C017251:1,2-oleoylphosphatidylcholine; D016650:Fluorescein-5-isothiocyanate; C018525:1,2-dimyristoylphosphatidylethanolamine