title: CO2 transport in normovolemic anemia: complete compensation and stability of blood CO2 tensions.
abstract: Isovolemic hemodilution does not appear to impair CO2 elimination nor cause CO2 retention despite the important role of red blood cells in blood CO2 transport. We studied this phenomenon and its physiological basis in eight New Zealand White rabbits that were anesthetized, paralyzed, and mechanically ventilated at a fixed minute ventilation. Isovolemic anemia was induced by simultaneous blood withdrawal and infusion of 6% hetastarch in sequential stages; exchange transfusions ranged from 15-30 ml in volume. Variables measured after each hemodilution included hematocrit (Hct), arterial and venous blood gases, mixed expired PCO2 and PO2, and blood pressure; also, O2 consumption, CO2 production, cardiac output (Q), and physiological dead space were calculated. Data were analyzed by comparison of changes in variables with changes in Hct and by using the model of capillary gas exchange described by Bidani (J. Appl. Physiol. 70: 1686-1699, 1991). There was complete compensation for anemia with stability of venous and arterial PCO2 between Hct values of 36 +/- 3 and 12 +/- 1%, which was predicted by the mathematical model. Over this range of hemodilution, Q rose 50%, and the O2 extraction ratio increased 61% without a decline in CO2 production or a rise in alveolar ventilation. The dominant compensations maintaining CO2 transport in normovolemic anemia include an increased Q and an augmented Haldane effect arising from the accompanying greater O2 extraction.
authors.affiliation: Department of Anesthesiology, University of Washington, Seattle, 98195, USA.
authors.forename: S
authors.initials: S
authors.lastname: Deem
authors.forename: M K
authors.initials: MK
authors.lastname: Alberts
authors.forename: M J
authors.initials: MJ
authors.lastname: Bishop
authors.forename: A
authors.initials: A
authors.lastname: Bidani
authors.forename: E R
authors.initials: ER
authors.lastname: Swenson
mesh_terms: D000740:Anemia / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D001784:Blood Gas Analysis; D001810:Blood Volume / Q000502:physiology; D002245:Carbon Dioxide / Q000097:blood / Q000378:metabolism*; D006438:Hemodilution; D006439:Hemodynamics / Q000502:physiology; D006706:Homeostasis / Q000502:physiology; D007700:Kinetics; D008954:Models, Biological; D010101:Oxygen Consumption / Q000502:physiology; D011659:Pulmonary Gas Exchange / Q000502:physiology; D011817:Rabbits
chemical_list: D002245:Carbon Dioxide