Protective effect against Rhodococcus equi infection in mice of IgG purified from horses vaccinated with virulence associated protein (VapA)-enriched antigens.
IgG was purified from horses immunized with repeated doses of virulence associated (VapA) enriched antigens extracted with Triton X-114 from the surface of a virulent strain of R. equi. This IgG were administered to mice immunosuppressed by prior treatment with indomethacin. Mice administered the higher dose were completely protected against intraperitoneal infection with R. equi; mice given the lower dose were partially protected. By contrast, mice administered concentrated nonimmune equine IgG were not protected. This study demonstrates that VapA may be an important antigen involved in humoral protective immunity in R. equi infections caused by foal virulent strains.
Fernandez
A S
AS
Buenos Aires Province Central University, Tandil, Argentina.
Prescott
J F
JF
Nicholson
V M
VM
D000193:Actinomycetales Infections / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary*; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines; D003902:Detergents; D004797:Enzyme-Linked Immunosorbent Assay; D006734:Horse Diseases; D006736:Horses; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification / Q000627:therapeutic use*; D007165:Immunosuppression Therapy; D007213:Indomethacin / Q000494:pharmacology; D008074:Lipoproteins / Q000276:immunology*; D051379:Mice; D017830:Octoxynol; D011092:Polyethylene Glycols; D016929:Rhodococcus equi / Q000276:immunology*; D037521:Virulence Factors
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D003902:Detergents; D007074:Immunoglobulin G; D008074:Lipoproteins; D037521:Virulence Factors; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D007213:Indomethacin