[Recombinant Thermus thermophilus His6-DNA polymerase with reverse transcriptase activity].
The Tth DNA polymerase genes from the thermophilic bacterium Thermus thermophilus (strans HB-8 and Tt-111) were cloned and expressed in Escherichia coli cells. The target protein contained a polyhistidine tag at the N-terminus that allowed its single-stage isolation by affinity chromatography on Ni-NTA-agarose. The isolated enzyme displayed both high DNA polymerase and reverse transcriptase activities.
Smirnov
Iu V
IuV
Chakhmakhcehva
O G
OG
Efimov
V A
VA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002846:Chromatography, Affinity; D004274:DNA, Recombinant; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016963:Thermus thermophilus / Q000201:enzymology*
D004274:DNA, Recombinant; D011994:Recombinant Proteins; C068508:Tth polymerase; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase