Epidermal localization and protective effects of topically applied superoxide dismutase.
Data from the literature, as well as our previous work, indicate a protective effect of superoxide dismutase (SOD) in topical application against UV-induced cutaneous damage. In the present article we show that pre-treatment of the skin with SOD protects against PUVA-induced inflammatory reactions not only in murine, but also in human skin. Using fluorescently labelled Cu,Zn SOD, epifluorescence microscopy and digital image processing, we demonstrate that the FITC fluorescence localizes in the stratum corneum and upper granulosa, as well as in the epidermal cell layer surrounding the lumina of the hair follicles. These findings were similar for murine and human skin. Since autofluorescence was eliminated by a special filter, it can be ascertained that the fluorescence observed in the tissues was due to FITC-labelled SOD.
Filipe
P
P
Oxygen Free Radical Research Group, Université Paris VI, France.
Emerit
I
I
Vassy
J
J
Rigaut
J P
JP
Martin
E
E
Freitas
J
J
Fernandes
A
A
D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D016650:Fluorescein-5-isothiocyanate; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D008814:Mice, Inbred NZB; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011701:PUVA Therapy / Q000009:adverse effects; D011855:Radiodermatitis / Q000209:etiology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects
D000975:Antioxidants; D005456:Fluorescent Dyes; D013482:Superoxide Dismutase; D016650:Fluorescein-5-isothiocyanate