Comparisons of CYP2D messenger RNA splice variant profiles in human lung tumors and normal tissues.
Allelic variants of the CYP2D6 gene, a member of the cytochrome P450 gene superfamily, have been implicated in susceptibility to lung carcinogenesis. Human breast CYP2D6 and CYP2D7P (from a pseudogene) mRNAs were previously reported to be expressed as a series of splice variants. In this study, the expression of full-length and splice variants of these mRNAs in human lung tissue and tumors are reported for the first time and are compared in order to probe the potential for differential CYP2D6 regulation in lung normal tissue and tumors. The splice variant profiles differed within the same individual, but no consistent differences were detected.
Huang
Z
Z
Department of Environmental Health and Toxicology, School of Public Health, University at Albany, State University of New York, 12201, USA.
Fasco
M J
MJ
Spivack
S
S
Kaminsky
L S
LS
D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; C102835:cytochrome P-450 CYP2D7P; D003577:Cytochrome P-450 Enzyme System; D019389:Cytochrome P-450 CYP2D6