High-resolution liquid chromatographic method using ultraviolet detection for determination of ondansetron in human plasma.
This paper describes a simple technique for extraction and a sensitive high-performance liquid chromatographic method for separation and quantitation of ondansetron in human plasma. The procedure involved liquid-liquid extraction of ondansetron from plasma, reversed-phase HPLC separation and ultraviolet detection at 305 nm. The internal standard method was applied for quantitation. The recovery of ondansetron was >85%. Linearity was good throughout the concentration range anticipated in human plasma from investigations in panic disorder (0.5-15 ng/ml, r2 ranging from 0.9953 to 0.9988). This method was applied to the determination of plasma concentrations of ondansetron in humans.
Dépôt
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University of Montreal, Faculty of Medicine, Department of Pharmacology, Quebec, Canada.
Leroux
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Caillé
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D000932:Antiemetics / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006801:Humans; D008297:Male; D017294:Ondansetron / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D012702:Serotonin Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet
D000932:Antiemetics; D012702:Serotonin Antagonists; D017294:Ondansetron