Hypertonic-hyperosmotic solution modifies myoglobin levels in early reperfusion after ischemic cardiac arrest. Experimental model.
To evaluate the effect of reperfusion with hypertonic-hyperosmotic solution, cardiectomy was performed in 25 New Zealand white rabbits. Seven isolated hearts were submitted to 30 min of global ischemia and reperfused with oxygenated buffer for 60 min. Myoglobin and isoenzyme MB of creatine kinase concentrations were each measured in the effluent 15 min, and values were correlated (r = 0.5011, p = 0.015). After this procedure, 18 isolated hearts were randomized in two groups. Hearts of group I were reperfused with hypertonic-hyperosmotic solution (NaCl 7.5% dextran 60,000 MW) diluted in oxygenated buffer, and group II with oxygenated buffer. Myoglobin and coronary flow were measured in both groups, group I showed lower levels of myoglobin (p = 0.0069) and higher coronary flow (p = 0.028) than group II. In conclusion, changes in myoglobin concentration in the heart effluent are more sensitive than changes in isoenzyme MB of creatine kinase; thus, evaluation of this parameter may be useful in the detection of ischemia reperfusion injury. Additionally, hypertonic-hyperosmotic solution improves the coronary flow and has a protective effect against ischemia-reperfusion injury.
Careaga
G
G
Hospital de Cardiologia, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México, D.F.
Argüero
R
R
D000818:Animals; D015415:Biomarkers; D003326:Coronary Circulation / Q000187:drug effects; D003402:Creatine Kinase / Q000032:analysis*; D003911:Dextrans / Q000494:pharmacology*; D005260:Female; D006323:Heart Arrest / Q000378:metabolism* / Q000473:pathology; D007527:Isoenzymes; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000473:pathology; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000378:metabolism; D009211:Myoglobin / Q000032:analysis*; D009994:Osmolar Concentration; D011817:Rabbits; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*
D015415:Biomarkers; D003911:Dextrans; D007527:Isoenzymes; D009211:Myoglobin; D012462:Saline Solution, Hypertonic; D003402:Creatine Kinase