Release of amino acids from fatty livers during organ harvest for transplantation.
Shortage of organ donors presents a perplexing problem in liver transplantation, and improved methods for evaluating the viability of organs prior to implantation are urgently needed. In the present study, the hypothesis was evaluated that grafts from fatty livers release more amino acids than non-fatty controls during organ harvest. Amino acids in graft rinse effluents at the time of harvest and after cold storage were measured by reverse-phase high performance liquid chromatography and compared with plasma aspartate aminotransferase (AST) levels and recipient survival. Twenty-four hours after transplantation of fatty livers, AST levels in recipient rats were increased more than two-fold compared to non-fatty controls (p < 0.01). Survival in the control group was 83 percent, whereas animals receiving fatty livers from ethanol-treated rats survived no longer than 7 days after transplantation (p < 0.05). The rate of release of amino acids from the liver explant was two-fold higher during the harvest procedure (0.5 h) than during the subsequent 23.5 hour cold storage period (435 +/- 70 vs. 186 +/- 14 nmol/ml/hr/g liver, p < 0.001). Further, in the early rinse effluent, amino acids were released about two-fold faster from fatty livers than from controls (p < 0.05). This study demonstrates that the release of amino acids from liver explants increases during the harvesting procedure and is about two-fold higher in fatty livers which fail after transplantation than in surviving controls. It is proposed that amino acid release from explants after organ harvest might serve as a useful marker to evaluate graft function prior to transplantation.
von Frankenberg
M
M
Department of Pharmacology, University of North Carolina, Chapel Hill 27599, USA.
Forman
D T
DT
Frey
W
W
Stachlewitz
R F
RF
Bunzendahl
H
H
Thurman
R G
RG
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005234:Fatty Liver / Q000378:metabolism* / Q000601:surgery*; D006085:Graft Survival / Q000187:drug effects; D007552:Isotonic Solutions / Q000032:analysis / Q000378:metabolism; D016031:Liver Transplantation / Q000276:immunology / Q000401:mortality; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D009927:Tissue and Organ Procurement
D000596:Amino Acids; D007552:Isotonic Solutions; D000431:Ethanol; D001219:Aspartate Aminotransferases