title: Recombinant plasmid mobilization between E. coli strains in seven sterile microcosms.
abstract: Transfer by mobilization of a pBR derivative recombinant plasmid lacking transfer functions (oriT+, tra-, mob-) from one E. coli K12 strain to another was investigated in seven sterile microcosms corresponding to different environments. These microcosms were chosen as representative of environments that genetically engineered microorganisms (GEMOs) encounter after accidental release, namely attached biomass in aquatic environments (biofilm), soil, seawater, freshwater, wastewater, mouse gut, and mussel gut, GEMOs survived in the same way as the host strains in all microcosms. Recombinant DNA mobilization occurred in the mouse gut, in sterile soil, and in biofilm. The plasmid transfer rates principally reflected the environmental conditions encountered in each microcosm.
authors.affiliation: Observatoire océanologique, Unité de recherche associée, Centre national de la recherche scientifique 2071, Banyuls-sur-mer, France. lebaron@arago.univ-perp.fr
authors.forename: P
authors.initials: P
authors.lastname: Lebaron
authors.forename: P
authors.initials: P
authors.lastname: Bauda
authors.forename: M C
authors.initials: MC
authors.lastname: Lett
authors.forename: Y
authors.initials: Y
authors.lastname: Duval-Iflah
authors.forename: P
authors.initials: P
authors.lastname: Simonet
authors.forename: E
authors.initials: E
authors.lastname: Jacq
authors.forename: N
authors.initials: N
authors.lastname: Frank
authors.forename: B
authors.initials: B
authors.lastname: Roux
authors.forename: B
authors.initials: B
authors.lastname: Baleux
authors.forename: G
authors.initials: G
authors.lastname: Faurie
authors.forename: J C
authors.initials: JC
authors.lastname: Hubert
authors.forename: P
authors.initials: P
authors.lastname: Normand
authors.forename: D
authors.initials: D
authors.lastname: Prieur
authors.forename: S
authors.initials: S
authors.lastname: Schmitt
authors.forename: J C
authors.initials: JC
authors.lastname: Block
mesh_terms: D000818:Animals; D018441:Biofilms; D049872:Bivalvia / Q000382:microbiology; D015169:Colony Count, Microbial; D003227:Conjugation, Genetic; D017753:Ecosystem; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D005818:Genetic Engineering; D051379:Mice; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D012988:Soil Microbiology; D014871:Water Microbiology