Different affinity of monoclonal antibodies for conserved neutralizing epitopes on two strains of rubella virus.
There is, apparently, only one serological type of rubella virus (RV) in the population, although several isolates exist with different characteristics. Some authors failed to detect significant differences among RV strains by neutralization, hemagglutination inhibition, and enzyme immunoassay using polyclonal and monoclonal antibodies, but differences in growth, plaque morphology, and temperature sensitivity between vaccine and wild-type strains were shown by Chantler et al. (3) With the purpose of analyzing the possible differences among several strains of RV, we studied the affinity constant of two monoclonal antibodies (MAbs) for two conserved neutralizing epitopes. Wild-type Cordoba (regional isolation of a post-natal infection) and RA 27/3 (vaccine) strains of RV were tested. H3 and H14 MAbs were generated against wild-type Cordoba strain. They defined two epitopes with conserved neutralizing and hemagglutinating activity on both strains. The affinity of the MAbs (expressed as the affinity constant), was greater for Cordoba strain than for RA 27/3. Analyzing the results obtained, we conclude that the neutralizing epitopes defined by our MAbs on E1 glycoprotein are conserved in the two strains, but react with significative different affinities. This could be a way to characterize antigenically different viral strains of the same serotype.
Cordoba
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Instituto de Virología J. M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba.
Grutadauria
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Cuffini
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C
Zapata
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MT
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000737:chemistry*; D000915:Antibody Affinity; D001667:Binding, Competitive / Q000276:immunology; D017124:Conserved Sequence / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006386:Hemagglutination Tests; D006825:Hybridomas / Q000737:chemistry / Q000378:metabolism; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012412:Rubella virus / Q000276:immunology*; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes