High-resolution physical mapping of a 6.7-Mb YAC contig spanning a region critical for the monosomy 21 phenotype in 21q21.3-q22.1.
Deletion of genes from the chromosome 21 region between APP and SOD1 is a potential cause of some of the major phenotypic features of monosomy 21 patients. Fine physical mapping helps identify potential candidate genes. After selecting nonchimeric YACs by FISH analysis, we determined their marker contents by PCR and hybridization studies. Fifteen YACs were chosen and mapped by restriction enzyme analysis and labeling of end fragments. We localized 55 markers, including 31 STSs, 10 YAC ends, and 4 NotI linking clones, along a 6.7-Mb contig. This map facilitates transcriptional analysis of this region and construction of ready-to-sequence contigs. Furthermore, FISH mapping of two patients with partial monosomy 21 using YAC and cosmid clones allowed us to define more accurately the telomeric border of the critical region between markers S226 and S213.
Orti
R
R
CNRS URA 1335, Faculte de Medecine Necker Enfants Malades, Paris, France.
Mégarbane
A
A
Maunoury
C
C
Van Broeckhoven
C
C
Sinet
P M
PM
Delabar
J M
JM
D000015:Abnormalities, Multiple / Q000235:genetics / Q000473:pathology; D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D017931:DNA Primers / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016324:Sequence Tagged Sites; D016615:Telomere / Q000235:genetics
D017931:DNA Primers; D005819:Genetic Markers