title: Differences in resistance to herpes simplex virus type 1 (HSV-1) among oligodendroglia derived from different strains of mice are determined after viral adsorption but prior to the expression of immediate early (IE) genes.
abstract: The nature of an innate cellular resistance to HSV-1 of cultured murine oligodendrocytes (OLs) in three strains of mice (C57BL/6J, Balb/cByJ and A/J) was investigated. The expression of immediate early (ICP4), early (ICP8) and late (gC) antigens in primary OL cultures was studied using an indirect immunofluorescence (IF) technique. HSV-1 infected OLs from C57BL/6J mice showed no viral antigens at 24 h post infection (p.i.) but rather a marked delay in antigen expression beginning at 60 h p.i. In contrast all three proteins were expressed in A/J OLs at 24 h p.i. while Balb/cByJ OLs showed an intermediate protein expression pattern. These results suggest that the innate cellular resistance to HSV-1 is determined prior to the expression of immediate early viral antigens. To further study these differences, the adsorption capacity between the three mouse strains was compared using dextran purified, [3H]thymidine labelled virus. No differences in HSV-1 adsorption were identified. Results from viral penetration studies approached statistical significance suggesting that penetration may be impaired in C57BL/6J and Balb/cByJ OLs when compared to A/J OLs and is likely fusion independent. The selective differences in HSV-1 resistance mediated by OLs, reflect differences in virus host cell interactions, that likely contribute to differences in mortality, viral spread, and the ability of virus to induce central nervous system (CNS) demyelination.
authors.affiliation: Department of Pathology and Laboratory Medicine, BC Children's Hospital, Vancouver, Canada.
authors.forename: E E
authors.initials: EE
authors.lastname: Thomas
authors.forename: A S
authors.initials: AS
authors.lastname: Lau
authors.forename: B
authors.initials: B
authors.lastname: Morrison
authors.forename: S U
authors.initials: SU
authors.lastname: Kim
authors.forename: L F
authors.initials: LF
authors.lastname: Kastrukoff
mesh_terms: D000327:Adsorption; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000096:biosynthesis; D002478:Cells, Cultured; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D017781:Genes, Immediate-Early; D006561:Herpes Simplex / Q000401:mortality / Q000503:physiopathology*; D017874:Immediate-Early Proteins / Q000096:biosynthesis; D007113:Immunity, Innate; D008561:Membrane Fusion; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009836:Oligodendroglia / Q000821:virology*; D018139:Simplexvirus / Q000502:physiology*; D013045:Species Specificity; D014157:Transcription Factors / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D004268:DNA-Binding Proteins; C045462:ICP8 protein, Simplexvirus; D017874:Immediate-Early Proteins; D014157:Transcription Factors; D014764:Viral Proteins; C048925:herpes simplex virus, type 1 protein ICP4