Micellar electrokinetic capillary chromatography of benzodiazepine antiepileptics and their desmethyl metabolites in blood.
A fast and reliable method for the MEKC separation and determination of benzodiazepine antipileptics and their active metabolites in serum has been developed, using a separation buffer composed of borate (pH 9.5), SDS (18 mM), and acetonitrile (14%) as an organic modifier. The method is sensitive enough to be used clinically with a precision of less than 3% for the analysis of nitrazepam, clonazepam, clobazam, diazepam and their desmethyl metabolites in serum.
Imazawa
M
M
National Institute of Neuroscience, NCNP, Tokyo, Japan.
Hatanaka
Y
Y
D000927:Anticonvulsants / Q000097:blood*; D001569:Benzodiazepines / Q000097:blood*; D002845:Chromatography / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008745:Methylation; D008823:Micelles; D008991:Monitoring, Physiologic / Q000379:methods; D015203:Reproducibility of Results; D013997:Time Factors
D000927:Anticonvulsants; D008823:Micelles; D001569:Benzodiazepines