Effects of synergistic signaling by phytochrome A and cryptochrome1 on circadian clock-regulated catalase expression.
Persistent oscillation in constant conditions is a defining characteristic of circadian rhythms. However, in plants transferred into extended dark conditions, circadian rhythms in mRNA abundance commonly damp in amplitude over two or three cycles to a steady state level of relatively constant, low mRNA abundance. In Arabidopsis, catalase CAT3 mRNA oscillations damp rapidly in extended dark conditions, but unlike catalase CAT2 and the chlorophyll a/b binding protein gene CAB, in which the circadian oscillations damp to low steady state mRNA abundance, CAT3 mRNA oscillations damp to high steady state levels of mRNA abundance. Mutational disruption of either phytochrome- or cryptochrome-mediated light perception prevents damping of the oscillations in CAT3 mRNA abundance and reveals strong circadian oscillations that persist for multiple cycles in extended dark conditions. Damping of CAT3 mRNA oscillations specifically requires phytochrome A but not phytochrome B and also requires the cryptochrome1 blue light receptor. Therefore, we conclude that synergistic signaling mediated through both phytochrome A and cryptochrome1 is required for damping of circadian CAT3 mRNA oscillations in extended dark conditions.
Zhong
H H
HH
Department of Biological Sciences, Dartmouth College, Gilman Laboratory, Hanover, New Hampshire 03755, USA.
Resnick
A S
AS
Straume
M
M
Robertson McClung
C
C
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins; D002374:Catalase / Q000096:biosynthesis* / Q000235:genetics; D002940:Circadian Rhythm; D056931:Cryptochromes; D003624:Darkness; D029721:Drosophila Proteins; D004357:Drug Synergism; D005136:Eye Proteins; D005420:Flavoproteins / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D008027:Light; D017956:Photoreceptor Cells, Invertebrate; D010834:Phytochrome / Q000502:physiology*; D051942:Phytochrome A; D010940:Plant Proteins / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D015398:Signal Transduction
D029681:Arabidopsis Proteins; C538962:CRY1 protein, Arabidopsis; D056931:Cryptochromes; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins; C497973:PHYA protein, Arabidopsis; D051942:Phytochrome A; D010940:Plant Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C119828:cry protein, Drosophila; D010834:Phytochrome; D002374:Catalase