title: A melibiose transporter and an operon containing its gene in Enterobacter cloacae.
abstract: We detected inducible melibiose transport activity in cells of Enterobacter cloacae IID977. H+, but not Na+, was found to be the coupling cation for this transporter. We cloned and sequenced the gene encoding the melibiose transporter. A homology search of a protein sequence database revealed that this melibiose transporter has high sequence similarity with the lactose transporter (LacY) and the raffinose transporter (RafB) and has some similarity with the melibiose transporter (MelB) of Escherichia coli.
authors.affiliation: Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Okazaki
authors.forename: X X
authors.initials: XX
authors.lastname: Jue
authors.forename: H
authors.initials: H
authors.lastname: Miyake
authors.forename: M
authors.initials: M
authors.lastname: Kuroda
authors.forename: T
authors.initials: T
authors.lastname: Shimamoto
authors.forename: T
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D015139:Blotting, Southern; D003001:Cloning, Molecular; D016972:Enterobacter cloacae / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008553:Melibiose / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D027981:Symporters
chemical_list: D026901:Membrane Transport Proteins; D027981:Symporters; C021548:melibiose permease; D008553:Melibiose