Influence of lipopolysaccharide and interleukin-2 on proliferation and synthesis of sulfated macromolecules in cultured rat glomerular epithelial cells.
OBJECTIVE
To investigate the effects of lipopolysaccharide (LPS) and human recombinant interleukin-2 (hrIL-2) on the synthesis of sulfated macromolecules in cultured rat GECs.


METHODS
With 3H-thymidine (3H-TdR) and 35S-Na2SO4 dual isotope labelling technique, the proliferation and synthesis of sulfated macromolecules in cultured rat GECs were estimated according to the 3H-TdR uptake and 35SO4(2-)-contents in cell layers respectively.


RESULTS
Both LPS (6.25-50.0 micrograms/L) and hrIL-2 (2.5-10.0 kU/L) decreased the uptake of 3H-TdR by GECs (P < 0.05). As for incorporation of 35SO4(2-) into cell layers, the results that both means and adjusted means in hrIL-2 groups were lower than those in controls (P < 0.05) implied that hrIL-2 could inhibit the synthesis of basement membrane HSPG while LPS hindered incorporation through its inhibitory effect on cell proliferation.


CONCLUSIONS
We may infer from these results that LPS-like proinflammatory mediators could increase the subpopulation of large pores in capillary wall through the reduction of cell-associated HSPG, and IL-2-like cytokines could contribute to the development of proteinuria through its inhibitory effect on the production of basement membrane HSPG in immune-mediated glomerular disorders.
Zhang
B
B
Department of Nephrology, Xijing Hospital, 4th Military Medical University, Xi'an.
Wang
B
B
Chen
Y
Y
Yang
J
J
Zhang
J
J
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000096:biosynthesis*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology
D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D007376:Interleukin-2; D008070:Lipopolysaccharides; D011509:Proteoglycans; D011994:Recombinant Proteins