title: Clostridium thermocellum JW20 (ATCC 31549) is a coculture with Thermoanaerobacter ethanolicus.
abstract: A PCR assay based on 16S rRNA sequence differences among four thermophilic anaerobic bacterial strains was used to demonstrate contamination of Clostridium thermocellum JW20 (ATCC 31549) with a Thermoanaerobacter ethanolicus strain. Therefore, we suggest that interpretation of experimental results with C. thermocellum JW20 be viewed with caution.
authors.affiliation: Department of Animal Sciences, University of Kentucky, Lexington 40546-0215, USA. milerbe@pop.uky.edu
authors.forename: M
authors.initials: M
authors.lastname: Erbeznik
authors.forename: C R
authors.initials: CR
authors.lastname: Jones
authors.forename: K A
authors.initials: KA
authors.lastname: Dawson
authors.forename: H J
authors.initials: HJ
authors.lastname: Strobel
mesh_terms: D001421:Bacteria, Anaerobic / Q000235:genetics* / Q000302:isolation & purification*; D003013:Clostridium / Q000235:genetics* / Q000302:isolation & purification*; D018920:Coculture Techniques; D016988:Gram-Positive Asporogenous Rods, Irregular / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D012988:Soil Microbiology
chemical_list: D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S