Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates.
Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.
Reese
C B
CB
Department of Chemistry, King's College London, Strand, London WC2R 2LS, UK.
Song
Q
Q
D000641:Ammonia / Q000737:chemistry*; D008623:Mercaptoethanol / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry*; D013873:Thionucleotides / Q000737:chemistry*
D013873:Thionucleotides; D008623:Mercaptoethanol; D013455:Sulfur; D000641:Ammonia