title: Immunocytochemistry controls using cell culture.
abstract: A continuing problem in cytology laboratories is the lack of adequate control material for immunocytochemical testing. Usual control procedures involve testing paraffin-embedded control materials along with the patient specimens. These control materials are fundamentally unlike cytologic preparations. We have developed a method to make control preparations for immunocytochemical analysis using cultured anaplastic cells with known antigenic features from commercial sources. Cell lines included melanoma, rhabdomyosarcoma, T-cell leukemia, and squamous-cell carcinoma. Modified Saccomano and acetone fixation coupled with the cytospin technique enabled good-quality preparations. Cell lines were tested with antibodies for HMB-45, actin, leukocyte common antigen (LCA) and cytokeratin, which avidin-biotin immunoperoxidase and diaminobenzidine (DAB) as chromogens. Our final preparations were easily interpretable with excellent morphologic preservation of cellular detail. Cultured cells provide a superior method for preparing almost unlimited numbers of control slides for immunocytochemistry for laboratories with access to a tissue culture facility.
authors.affiliation: Department of Pathology, University of Wisconsin, Madison 53706, USA. dkurtycz@facstaff.wisc.edu
authors.forename: D F
authors.initials: DF
authors.lastname: Kurtycz
authors.forename: R
authors.initials: R
authors.lastname: Logrono
authors.forename: M
authors.initials: M
authors.lastname: Leopando
authors.forename: A
authors.initials: A
authors.lastname: Slattery
authors.forename: S L
authors.initials: SL
authors.lastname: Inhorn
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D014407:Tumor Cells, Cultured