title: Enzymatic hydrolysis of the conjugate of vitamin D and related compounds.
abstract: The monoglucuronides of vitamin D, 25-hydroxyvitamin D and the corresponding pro-forms were subjected to enzymatic hydrolysis using beta-glucuronidase, and substrate specificities were found in the examined enzymes originating from different sources, which were determined using reversed-phase high-performance liquid chromatography with UV detection. The enzymatic hydrolysis of the corresponding monosulfates was also examined using the same system.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Mitamura
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtake
authors.forename: I
authors.initials: I
authors.lastname: Nakatani
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005966:Glucuronidase / Q000737:chemistry*; D006868:Hydrolysis; D013379:Substrate Specificity; D013429:Sulfatases / Q000737:chemistry*; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D014807:Vitamin D; C104450:25-hydroxyvitamin D; D013429:Sulfatases; D005966:Glucuronidase