CD4 and CD8 expressions in African green monkey helper T lymphocytes: implication for resistance to SIV infection.
We found that most peripheral CD4 cells co-express a low density of CD8 alpha antigen in African green monkeys (AGM). Further, the cell surface expression of CD4 and the expression of CD4 mRNA underwent a decrease when purified CD4CD8low cells were cultured with mitogen and IL-2. These observations suggest that AGM CD4 cells are subject to loss of CD4 expression after lymphocyte activation. Part of the peripheral CD8 fraction exhibited a significant helper activity which suggested the phenotypic conversion in helper T cells from CD4+ to CD4- in vivo. Simian immunodeficiency virus (SIV) grew well in CD4 panning cells following SIV infection. In contrast, CD4CD8low cells were resistant to SIV infection after their conversion to CD4- cells.
Murayama
Y
Y
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki-ken, Japan.
Amano
A
A
Mukai
R
R
Shibata
H
H
Matsunaga
S
S
Takahashi
H
H
Yoshikawa
Y
Y
Hayami
M
M
Noguchi
A
A
D000818:Animals; D015704:CD4 Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology; D016827:CD8 Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D007113:Immunity, Innate; D008213:Lymphocyte Activation; D012333:RNA, Messenger / Q000096:biosynthesis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism* / Q000821:virology
D015704:CD4 Antigens; D016827:CD8 Antigens; D012333:RNA, Messenger