Digoxinlike immunoreactive factor isolated from human pleural fluid is structurally similar to digoxin.
To further define the chemical structure of human endogenous digoxinlike immunoreactive factors (DLIF) we used human pleural effusions as a source of the substance. Digoxinlike immunoreactive factor activity was detected by radioimmunoassay in the pleural fluid of each of four patients; average concentration was 0.35 ng/mL. The chemical profile of DLIF was determined by initial extraction and concentration of DLIF by ion exchange chromatography followed by reverse phase-high-pressure liquid chromatography (RP-HPLC) separation and purification. Using high-pressure liquid chromatography cochromatography of DLIF, together with several radioactively marked glycosides, we observed a single peak of DLIF activity that was chromatographically identical to digoxin. The present study further supports the recent finding that DLIF is related structurally to the cardiac glycosides, and for the first time it has been proven that DLIF is present in pleural fluids.
Weinberg
U
U
Endocrine Unit, Meir Hospital, Sapir Medical Center, Kfar Saba, Israel. uweinbe@ibm.net
Dolev
S
S
Shapiro
M S
MS
Shilo
L
L
Rabinowitz
R
R
Shenkman
L
L
D002298:Cardenolides; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004077:Digoxin / Q000737:chemistry*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism; D008297:Male; D010996:Pleural Effusion / Q000378:metabolism*; D051437:Renal Insufficiency / Q000378:metabolism; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification*
D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D004077:Digoxin