Inactivated poliovirus vaccine protects transgenic poliovirus receptor mice against type 3 poliovirus challenge.
Transgenic (Tg) mice expressing the human poliovirus receptor (PVR) were vaccinated with inactivated poliovirus vaccine (IPV) and evaluated for induced immunity against type 3 poliomyelitis. One injection of monovalent type 3 IPV elicited protective immunity against wild-type poliovirus. In contrast, 2 injections of trivalent IPV were required for protection. Neutralizing antibody response and protection were vaccine dose-dependent. Administration of polio-immune mouse plasma protected unimmunized mice, demonstrating that neutralizing antibody was sufficient for immunity. IPV heated to remove its D antigen component did not induce protection in Tg PVR mice. IPV derived from a wild-type poliovirus strain gave better protection against wild-type viral challenge than IPV derived from an attenuated poliovirus strain. The newly developed Tg PVR mouse-protection test may be useful in evaluating existing IPV potency tests and for attempts to improve formulations of trivalent IPV or combined vaccines for childhood immunization schedules.
Taffs
R E
RE
Food and Drug Administration, Rockville, Maryland, USA.
Chernokhvostova
Y V
YV
Dragunsky
E M
EM
Nomura
T
T
Hioki
K
K
Beuvery
E C
EC
Fitzgerald
E A
EA
Levenbook
I S
IS
Asher
D M
DM
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D007116:Immunization, Passive; D008565:Membrane Proteins; D051379:Mice; D008822:Mice, Transgenic; D009500:Neutralization Tests; D011051:Poliomyelitis / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control*; D017955:Poliovirus / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000008:administration & dosage / Q000276:immunology*; D011991:Receptors, Virus / Q000235:genetics*; D015164:Vaccines, Inactivated / Q000032:analysis / Q000276:immunology
D008565:Membrane Proteins; D011054:Poliovirus Vaccine, Inactivated; D011991:Receptors, Virus; D015164:Vaccines, Inactivated; C066100:poliovirus receptor