Analysis of urinary and faecal porphyrin excretion patterns in human porphyrias by fast atom bombardment mass spectrometry.
We report a new method for obtaining urinary and faecal porphyrin excretion patterns in human porphyrias based on fast atom bombardment mass spectrometry (FAB-MS). Porphyrins were esterified and extracted from urine or faeces as their methyl esters for analysis by FAB-MS. The protonated pseudo-molecular ion [M + H]+ observed for each porphyrin is characteristic of that porphyrin, thus allowing a mixture of porphyrins to be analysed without the need for chromatographic separation. By using tandem MS, identification and characterisation of unknown porphyrins can be achieved. The urinary and faecal porphyrin excretion patterns from various porphyric patients obtained by FAB-MS are in good agreement with those analysed by TLC or HPLC methods.
Luo
J
J
MRC Toxicology Unit, University of Leicester, UK.
Lamb
J H
JH
Lim
C K
CK
D005243:Feces / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D011164:Porphyrias / Q000378:metabolism* / Q000652:urine; D011166:Porphyrins / Q000378:metabolism* / Q000652:urine; D016339:Spectrometry, Mass, Fast Atom Bombardment
D011166:Porphyrins