Isolation and structural characterization of pituitary adenylate cyclase activating polypeptide (PACAP)-like peptide from the brain of a teleost, stargazer, Uranoscopus japonicus.
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a novel neuropeptide consisting of 38-residue (PACAP 1-38) and a truncated form with 27 residues (PACAP 1-27) that plays several roles in tetrapods. We isolated a highly purified PACAP-like peptide from the brain of a teleost, the stargazer, by extracting of acetone-dried powder with acetic acid followed by high-performance liquid chromatography (HPLC) on gel-filtration, cation-exchange, and reverse-phase columns. Purification was monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting analysis using an anti-PACAP 1-27 antiserum. The PACAP-like peptide thus obtained had a molecular mass of 4,623, determined by mass spectrometry, and its amino acid sequence showed 89 and 87% identity with those of ovine and frog PACAPs, respectively. These results indicate that a PACAP-like peptide, which is a highly homologous with tetrapod PACAP, is present in the teleost brain.
Matsuda
K
K
Department of Biology, Faculty of Science, Toyama University, Japan.
Takei
Y
Y
Katoh
J
J
Shioda
S
S
Arimura
A
A
Uchiyama
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009479:Neuropeptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000502:physiology; D010473:Perciformes; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide