Poliovirus protein 2BC increases cytosolic free calcium concentrations.
Poliovirus-infected cells undergo an increase in cytoplasmic calcium concentrations from the 4th h postinfection. The protein responsible for this effect was identified by the expression of different poliovirus nonstructural proteins in HeLa cells by using a recombinant vaccinia virus system. Synthesis of protein 2BC enhances cytoplasmic calcium concentrations in a manner similar to that observed in poliovirus-infected cells. To identify the regions in 2BC involved in modifying cytoplasmic calcium levels, several 2BC variants were generated. Regions present in both 2B and 2C are necessary to augment cellular free calcium levels. Therefore, in addition to inducing proliferation of membranous vesicles, poliovirus protein 2BC also alters cellular calcium homeostasis.
Aldabe
R
R
Centro de Biología Molecular, UAM-CSIC, Universidad Autonoma de Madrid, Canto Blanco, Spain.
Irurzun
A
A
Carrasco
L
L
D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000502:physiology*; D003600:Cytosol / Q000378:metabolism; D006367:HeLa Cells; D006706:Homeostasis; D006801:Humans; D017361:Viral Nonstructural Proteins / Q000502:physiology*; D014779:Virus Replication
C091441:2BC protein, Poliovirus; D002352:Carrier Proteins; D017361:Viral Nonstructural Proteins; D002118:Calcium