Interleukin-8 and chemotactic activity of middle ear effusions.
The importance of interleukin (IL)-8 in the chemotactic activity of middle ear effusions (MEEs) was evaluated. There was a significantly higher IL-8 concentration in MEEs of children with acute otitis media (AOM) (n = 17; 136 ng/mL) than in children with otitis media with effusion (OME) (n = 28; 65 ng/mL). The IL-8 concentration in MEEs with bacteria (149 ng/mL) was significantly higher than in MEEs without bacteria (66 ng/mL). MEEs from children with AOM and OME had equally higher chemotactic activity than the diluent alone (23.3% and 24.8% vs. 9.2%). The chemotactic activity was not altered by the presence of bacteria nor did it correlate with IL-8 concentration. Fractionation of MEEs by gel chromatography demonstrated that the main chemotactic activity could clearly be separated from the IL-8 activity, thus excluding IL-8 as a main chemotactic component in MEEs.
Storgaard
M
M
Department of Medicine and Infectious Diseases, Marselisborg Hospital, Aarhus, Denmark.
Larsen
K
K
Blegvad
S
S
Nødgaard
H
H
Ovesen
T
T
Andersen
P L
PL
Obel
N
N
D001424:Bacterial Infections / Q000150:complications; D002633:Chemotaxis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D002850:Chromatography, Gel; D004432:Ear, Middle / Q000276:immunology / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D016209:Interleukin-8 / Q000032:analysis* / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000276:immunology; D010033:Otitis Media / Q000276:immunology; D010034:Otitis Media with Effusion / Q000276:immunology*
D016209:Interleukin-8