title: Anti-insect toxin 5 (AaIT5) from Androctonus australis.
abstract: An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose < 1.8 microg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiurus quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).
authors.affiliation: Department of Entomology, University of California, Davis 95616, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Y M
authors.initials: YM
authors.lastname: Lee
authors.forename: E
authors.initials: E
authors.lastname: Lehmberg
authors.forename: R
authors.initials: R
authors.lastname: Herrmann
authors.forename: R
authors.initials: R
authors.lastname: Herrmann
authors.forename: H
authors.initials: H
authors.lastname: Moskowitz
authors.forename: A D
authors.initials: AD
authors.lastname: Jones
authors.forename: B D
authors.initials: BD
authors.lastname: Hammock
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004175:Diptera / Q000196:embryology; D007814:Larva / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D009036:Moths; D010446:Peptide Fragments / Q000302:isolation & purification; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification* / Q000633:toxicity; D017386:Sequence Homology, Amino Acid; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010446:Peptide Fragments; D012604:Scorpion Venoms; C106838:scorpion toxin AaIT5