Lack of evidence of human herpesvirus 8 DNA sequences in HIV-negative patients with various lymphoproliferative disorders of the skin.
Human herpesvirus 8 (HHV-8) is a new virus which has been reported in Kaposi's sarcoma and some lymphoproliferative disorders such as Castleman's disease and body-cavity-based lymphoma. Because HHV-8 shares homology with Epstein-Barr virus (EBV), we searched for the presence of HHV-8 DNA sequences in various cutaneous T- and B-cell lymphoma by the polymerase chain reaction (PCR). Forty-seven HIV-negative patients with cutaneous lymphoma or large plaque parapsoriasis were enrolled in the study. For the detection of HHV-8 DNA sequences we used PCR followed by a hybridization with a digoxigenin-labelled probe and nested-PCR. HHV-8 DNA sequences could only be detected in a patient with large plaque parapsoriasis. Our study does not suggest any direct implication of HHV-8 in the pathogenesis of most cutaneous lymphoma. Serological studies will be helpful to appreciate if there is an epidemiological link between HHV-8 and cutaneous lymphomas.
Dupin
N
N
Department of Dermatology, Hôpital Tarnier-Cochin, France.
Franck
N
N
Calvez
V
V
Gorin
I
I
Grandadam
M
M
Huraux
J M
JM
Leibowitch
M
M
Agut
H
H
Escande
J P
JP
D004279:DNA, Viral / Q000032:analysis*; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D008223:Lymphoma / Q000821:virology*; D016393:Lymphoma, B-Cell / Q000821:virology; D016399:Lymphoma, T-Cell / Q000821:virology; D010267:Parapsoriasis / Q000821:virology*; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000821:virology*
D004279:DNA, Viral