title: Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae.
abstract: The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher than 25 degrees C, it catalyzes acyl transfer reactions on properly structured thiolesters, and it binds penicillin with high affinity.
authors.affiliation: Centre d'Ingénierie des Protéines, Institut de Chimie, Université dede Liège, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Lepage
authors.forename: P
authors.initials: P
authors.lastname: Dubois
authors.forename: T K
authors.initials: TK
authors.lastname: Ghosh
authors.forename: B
authors.initials: B
authors.lastname: Joris
authors.forename: S
authors.initials: S
authors.lastname: Mahapatra
authors.forename: M
authors.initials: M
authors.lastname: Kundu
authors.forename: J
authors.initials: J
authors.lastname: Basu
authors.forename: P
authors.initials: P
authors.lastname: Chakrabarti
authors.forename: S T
authors.initials: ST
authors.lastname: Cole
authors.forename: M
authors.initials: M
authors.lastname: Nguyen-Distèche
authors.forename: J M
authors.initials: JM
authors.lastname: Ghuysen
mesh_terms: D000215:Acylation; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003360:Cosmids; D004926:Escherichia coli / Q000235:genetics; D006602:Hexosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002267:Muramoylpentapeptide Carboxypeptidase; D009166:Mycobacterium leprae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000378:metabolism*; D010458:Peptidyl Transferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013696:Temperature; D014169:Transformation, Bacterial
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D011994:Recombinant Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase