Isolation of transforming growth factor-beta2 cDNA from a fish, Cyprinus carpio by RT-PCR.
Transforming Growth Factors-beta (TGF-betas) have been described in many vertebrate species of amphibians, aves and mammals. In this report we demonstrate the presence of TGF-beta2 in pisces. TGF-beta2 has been cloned from a fish, Cyprinus carpio, by RT-PCR using degenerate oligonucleotide primers. Sequence analysis of the amplified product and alignment of the deduced amino acid sequence with the human TGF-beta2 amino acid sequence revealed 81% and 93% identity in the precursor and the mature regions, respectively. The northern blot analysis of fish heart RNA shows a major messenger RNA species of about 8.0 kb and two messages of very low abundance of about 5.0 kb and 4.0 kb. The identification of TGF-beta2 isoform in Pisces and it's high degree of homology with the mammalian isoform suggests that among all TGF-beta isoforms, TGF-beta2 is the most conserved during evolution.
Sumathy
K
K
Indian Institute of Science Center for Reproductive Biology and Molecular Endocrinology, Indian Institute of Science, Bangalore.
Desai
K V
KV
Kondaiah
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002347:Carps / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000302:isolation & purification
D018076:DNA, Complementary; D016212:Transforming Growth Factor beta