title: Highly efficient control of iron-containing nitrile hydratases by stoichiometric amounts of nitric oxide and light.
abstract: The reaction of two iron-containing nitrile hydratases (NHase) with NO has been studied: NHase from Rhodococcus sp. R312, which is probably similar to the photosensitive N771 NHase, and the new NHase from Comamonas testosteroni NI1 whose aminoacid sequence is quite different from those of BR312 and N771 NHases. Both enzymes are equally inactivated after addition of stoichiometric amounts of NO added as an anaerobic solution or produced in situ under physiological conditions by a rat brain NO-synthase. Both enzymes are reactivated by photoirradiation, and two cycles of NO inactivation/photoactivation can be performed without significant loss of activity. Both iron-containing NHases have a high affinity for NO, similar to that of methemoglobin.
authors.affiliation: Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques (URA 400 CNRS), Université Paris V, France.
authors.forename: D
authors.initials: D
authors.lastname: Bonnet
authors.forename: I
authors.initials: I
authors.lastname: Artaud
authors.forename: C
authors.initials: C
authors.lastname: Moali
authors.forename: D
authors.initials: D
authors.lastname: Pétré
authors.forename: D
authors.initials: D
authors.lastname: Mansuy
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D004789:Enzyme Activation / Q000187:drug effects; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology; D006836:Hydro-Lyases / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008027:Light; D008706:Methemoglobin / Q000378:metabolism; D009569:Nitric Oxide / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010777:Photochemistry; D051381:Rats; D012240:Rhodococcus / Q000201:enzymology
chemical_list: D009569:Nitric Oxide; D008706:Methemoglobin; D007501:Iron; D019001:Nitric Oxide Synthase; D006836:Hydro-Lyases; C048831:nitrile hydratase