title: A subpopulation of reactive astrocytes at the immediate site of cerebral cortical injury.
abstract: We have identified an early-appearing intermediate filament-associated protein (IFAP-70/280 kDa) in radial glia and their immediate derivatives. This IFAP is absent in the adult CNS. In this study, we examined the reexpression of this early glial differentiation trait in rat reactive astrocytes induced by stab injury of the cerebrum. Double-label immunofluorescence microscopy demonstrated that by 36 h postlesion, IFAP-70/280 kDa was present in a few GFAP-positive astrocytes in the area adjacent to the wound. As the gliotic reaction progressed, the number of IFAP-positive reactive astrocytes increased and by 5-6 days postlesion, IFAP-70/280 kDa was present in most of the hypertrophied astrocytes in tissue immediately adjacent to the wound. By 8 days postlesion, while the number of IFAP-negative reactive astrocytes away from the wound diminished, the IFAP-containing reactive astrocytes close to the wound persisted. Concurrently, they began to change from a stellate form to an elongated shape, with their longitudinal axes radiating from the wound. The immunoreactivity of this IFAP started to diminish at 20 days postlesion, and by 30 days postlesion, it was not observed in the remaining gliotic cells. These results demonstrate that reactive astrocytes induced by stab-wound injury can be divided into two subtypes: persistent IFAP-70/280 kDa-containing cells which are close to the wound in the area of the glial scar and transient IFAP-70/280 kDa-negative cells which are farther from the wound. The reappearance of IFAP-70/280 kDa also suggests that some reactive astrocytes have the capacity to recapitulate early developmental stages.
authors.affiliation: Department of Anatomy, University of Illinois at Chicago 60612, USA.
authors.forename: H Y
authors.initials: HY
authors.lastname: Yang
authors.forename: N
authors.initials: N
authors.lastname: Lieska
authors.forename: V
authors.initials: V
authors.lastname: Kriho
authors.forename: C M
authors.initials: CM
authors.lastname: Wu
authors.forename: G D
authors.initials: GD
authors.lastname: Pappas
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000145:classification / Q000473:pathology*; D015415:Biomarkers / Q000032:analysis*; D001930:Brain Injuries / Q000473:pathology*; D002540:Cerebral Cortex / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005911:Gliosis / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014951:Wounds, Stab / Q000473:pathology*
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein