title: Enantiomer separation of denopamine by capillary electrophoresis with charged and uncharged cyclodextrins.
abstract: Direct separation of enantiomers of denopamine was investigated by capillary electrophoresis employing charged and uncharged cyclodextrin (CD) derivatives. Uncharged beta-type CDs, having hydrophobic groups, were essential for the enantioseparation of denopamine; of these, especially dimethyl-beta-CD was effective. Among charged CDs, gamma-type as well as beta-type CDs were found effective for the enantioseparation of denopamine. Reversal of migration order of R-form (active) and S-form enantiomers was investigated by using two types of coated capillaries: (i) an amine capillary with an inner wall coated with dimethylamino groups, and (ii) a polyacrylamide-coated capillary. Manipulation of migration order could be easily performed by selecting suitable capillaries, buffer pH, and CDs.
authors.affiliation: Analytical Research Laboratory, Tanabe Seiyaku Co., Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ishibuchi
authors.forename: S
authors.initials: S
authors.lastname: Izumoto
authors.forename: H
authors.initials: H
authors.lastname: Nishi
authors.forename: T
authors.initials: T
authors.lastname: Sato
mesh_terms: D003505:Cyclodextrins / Q000737:chemistry*; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004983:Ethanolamines / Q000737:chemistry / Q000302:isolation & purification*; D008968:Molecular Conformation
chemical_list: D003505:Cyclodextrins; D004983:Ethanolamines; C037293:denopamine