Alternative splicing of the rabbit beta-globin pre-mRNA in vivo after transient transfection of myoblast and myotube cells.
The relationship between preferences among alternative 5' splice sites and their sequences was investigated using as model mouse myoblasts and myotubes after transient transfection with the rabbit beta-globin gene. The preferences for the use of two different 5' splice sites, acting with different efficiencies to direct splicing in vivo are reported. The predominant selection of the upstream splice site has been shown in normal mouse myoblasts. In the case of differentiated myotubes the downstream splice was 1.4 times better used. The results indicate that there were differences in the preferences for the use of the two alternative splice sites between non-differentiated and terminally differentiated cells, within the same-cell line.
Pironcheva
G L
GL
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Russev
G
G
D017398:Alternative Splicing; D000818:Animals; D002460:Cell Line; D005914:Globins / Q000235:genetics*; D051379:Mice; D009132:Muscles / Q000166:cytology; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000235:genetics*; D011817:Rabbits; D014162:Transfection
D012322:RNA Precursors; D005914:Globins