title: Levels of L-methionine S-adenosyltransferase activity in erythrocytes and concentrations of S-adenosylmethionine and S-adenosylhomocysteine in whole blood of patients with Parkinson's disease.
abstract: In the present study, levels of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in whole blood as well as L-methionine S-adenosyltransferase (MAT) activity in erythrocytes were assayed in a series of 20 patients with Parkinson's disease and 12 healthy control subjects. A significant difference was found with regard to SAM levels between patients and controls, with the detected levels being 383.1 +/- 41.5 nM for the parkinsonian patients and 680.6 +/- 30.9 nM for the controls. With regard to SAH, we found no difference between the groups. The catalytic activity of MAT was increased by 30% in patients compared to controls, with the Vmax for methionine being 17.9 +/- 3.7 and 13.9 +/- 2.2 pmol/mg/h, respectively.
authors.affiliation: Department of Medical Pharmacology, Uppsala University, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Cheng
authors.forename: C
authors.initials: C
authors.lastname: Gomes-Trolin
authors.forename: S M
authors.initials: SM
authors.lastname: Aquilonius
authors.forename: A
authors.initials: A
authors.lastname: Steinberg
authors.forename: C
authors.initials: C
authors.lastname: Löfberg
authors.forename: J
authors.initials: J
authors.lastname: Ekblom
authors.forename: L
authors.initials: L
authors.lastname: Oreland
mesh_terms: D000978:Antiparkinson Agents / Q000008:administration & dosage; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry / Q000201:enzymology*; D005260:Female; D006801:Humans; D007980:Levodopa / Q000008:administration & dosage; D008297:Male; D008716:Methionine Adenosyltransferase / Q000378:metabolism*; D008875:Middle Aged; D010300:Parkinson Disease / Q000188:drug therapy / Q000378:metabolism*; D012435:S-Adenosylhomocysteine / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism*
chemical_list: D000978:Antiparkinson Agents; D007980:Levodopa; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; D008716:Methionine Adenosyltransferase