title: tTGase/G alpha h protein expression inhibits adenylate cyclase activity in Balb-C 3T3 fibroblasts membranes.
abstract: Stably transfected Balb-C 3T3 fibroblasts (clone 5), overexpressing a catalytically active tissue transglutaminase, showed a basal adenylate cyclase activity lower than control cells (clone 1). Several modulators of the adenylate cyclase activity (forskolin, Mn2+ and pertussis toxin) showed the existence of a marked negative control on the adenylate cyclase activity present in clone 5 cells. Very interestingly, this same marked negative control was also found in a Balb-C 3T3 fibroblast clone stably transfected with a mutagenized human tissue transglutaminase (mut277 cys > ser) virtually devoid of transglutaminase catalytic activity (clone Ser). Conversely, a significant increase of the adenylate cyclase activity was observed in bovine aortic endothelial cells after the lowering of tissue transglutaminase expression levels by the transfection of an eukaryotic expression vector containing the gene for tissue transglutaminase in antisense orientation. All these findings suggest a possible role for type II tissue transglutaminase as a negative modulator of the adenylate cyclase activity in different cell types, beside its transglutaminase enzyme activity.
authors.affiliation: Department of Biochemistry and Biophysics, Second University of Naples, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Gentile
authors.forename: R
authors.initials: R
authors.lastname: Porta
authors.forename: E
authors.initials: E
authors.lastname: Chiosi
authors.forename: A
authors.initials: A
authors.lastname: Spina
authors.forename: F
authors.initials: F
authors.lastname: Valente
authors.forename: R
authors.initials: R
authors.lastname: Pezone
authors.forename: P J
authors.initials: PJ
authors.lastname: Davies
authors.forename: A
authors.initials: A
authors.lastname: Alaadik
authors.forename: G
authors.initials: G
authors.lastname: Illiano
mesh_terms: D016475:3T3 Cells; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D003432:Cross-Linking Reagents; D004730:Endothelium, Vascular / Q000201:enzymology; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D014162:Transfection; D011503:Transglutaminases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D003432:Cross-Linking Reagents; D011503:Transglutaminases