Comparison of susceptibility to apoptosis induced by rhTNF-alpha and cycloheximide between human circulating and exudated neutrophils.
To determine whether exudated neutrophils differ from circulating ones in their apoptosis, rhTNF-alpha plus cycloheximide-induced apoptosis in human salivary neutrophils was compared to that in human neutrophils in peripheral blood. Concomitant treatment of peripheral blood neutrophils with rhTNF-alpha and cycloheximide-induced apoptosis in blood neutrophils within 3 hr, as evaluated both by light microscopic changes characteristic to apoptosis and by DNA fragmentation, whereas the same treatment failed to induce any apoptosis in salivary neutrophils. These results indicate that the exudation of neutrophils from blood into tissue is associated with marked changes in their functions such as alteration in their sensitivity to apoptosis-inducing stimuli.
Niwa
M
M
Department of Pharmacology, Gifu University School of Medicine, Tsukasamachi, Japan. mniwa@cc.gifu-u.ac.jp
Hara
A
A
Kanamori
Y
Y
Kohno
K
K
Yoshimi
N
N
Mori
H
H
Uematsu
T
T
D017209:Apoptosis; D003513:Cycloheximide / Q000494:pharmacology*; D053938:DNA Fragmentation; D004357:Drug Synergism; D006801:Humans; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D012463:Saliva / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003513:Cycloheximide