title: The uvsF gene region in Aspergillus nidulans codes for a protein with homology to DNA replication factor C.
abstract: The UV-sensitive mutant uvsF201 of Aspergillus nidulans shows increased spontaneous and UV-induced mutation and generally resembles mutants defective in nucleotide excision repair (NER). Fully-complementing uvsF clones were isolated from cosmid and cDNA libraries for sequencing. The uvsF gene is approximately 3.75 kb long and codes for a predicted polypeptide of 1092 amino acids (aa). Three small introns are clustered early in the coding region of the protein. A major part of the sequence shows homology to human, mouse and yeast RFC1 genes which code for the large subunit of the DNA replication factor C. The uvsF gene product may therefore function primarily in general DNA replication but in addition be required for the replication step of DNA repair. Extended sequencing of the uvsF gene region identified a second closely adjacent gene of unknown function which is divergently transcribed from a small (0.2 kb) intergenic promoter region.
authors.affiliation: Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada. kafer@darwin.mbb.sfu.ca
authors.forename: E
authors.initials: E
authors.lastname: Kafer
authors.forename: G
authors.initials: G
authors.lastname: May
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000737:chemistry* / Q000235:genetics* / Q000528:radiation effects; D001483:Base Sequence; D003001:Cloning, Molecular; D004260:DNA Repair; D004261:DNA Replication; D018076:DNA, Complementary / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D005656:Fungal Proteins; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D018398:Homeodomain Proteins; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D019253:Proto-Oncogene Proteins c-bcl-2; D051818:Replication Protein C; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: C096816:BCL2-related protein A1; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D018398:Homeodomain Proteins; C096063:MATA1 protein, S cerevisiae; D015778:Minor Histocompatibility Antigens; D019253:Proto-Oncogene Proteins c-bcl-2; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C107007:UvsF protein, Emericella nidulans; D051818:Replication Protein C