title: VP7: an attachment protein of bluetongue virus for cellular receptors in Culicoides variipennis.
abstract: The importance of VP7 of bluetongue virus (BTV) in the binding of BTV to membrane proteins of the BTV vector Culicoides variipennis was investigated. Core BTV particles, prepared from whole viruses, lacked outer proteins VP2 and VP5 and had VP7 exposed. More core particles and whole viruses bound to membrane preparations of adults of C. variipennis and KC cells, which were cultured from this vector insect, than to membrane preparations of Manduca sexta larvae. More core particles than whole viruses bound to membrane preparations of adults of C. variipennis and KC cells. Polyclonal anti-idiotypic antibodies (anti-Id), which were made against an antigen-combining region of an anti-BTV-10 VP7 antibody and functionally mimicked VP7, bound more to the membrane preparations of adults of C. variipennis and KC cells, and less to cytosol preparations. In Western overalay analysis, the Culicoides plasma membrane preparation reduced binding of an anti-VP7 monoclonal antibody to VP7. Whole and core BTV particles and the anti-Id bound to a membrane protein with a molecular mass of 23 kDa that was present predominantly in membrane preparations of adults of C. variipennis and KC cells. This protein was present in much lower concentrations in membrane preparations of C6/36 and DM-2 insect cells.
authors.affiliation: Arthropod-Borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, WY 82071-3965, USA.
authors.forename: G
authors.initials: G
authors.lastname: Xu
authors.forename: W
authors.initials: W
authors.lastname: Wilson
authors.forename: J
authors.initials: J
authors.lastname: Mecham
authors.forename: K
authors.initials: K
authors.lastname: Murphy
authors.forename: E M
authors.initials: EM
authors.lastname: Zhou
authors.forename: W
authors.initials: W
authors.lastname: Tabachnick
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001820:Bluetongue virus / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002519:Ceratopogonidae / Q000378:metabolism*; D006224:Cricetinae; D004331:Drosophila melanogaster; D007130:Immunoglobulin Idiotypes / Q000378:metabolism; D018402:Manduca; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D011991:Receptors, Virus / Q000737:chemistry / Q000378:metabolism*; D014758:Viral Core Proteins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D008565:Membrane Proteins; D011991:Receptors, Virus; D014758:Viral Core Proteins; C066862:VP7 protein, orbivirus