title: Hepatitis C virus genotypes in Southern Brazil.
abstract: The prevalence of hepatitis C virus (HCV) genotypes in Southern Brazil was studied in the plasma of 100 HCV-RNA-positive patients attended in Porto Alegre, South of Brazil. Reverse transcription-polymerase chain reaction (RT-PCR) products from the 5' noncoding region were double digested with RsaI-HaeIII and BstNI-HinfI and analyzed by restriction fragment length polymorphism (RFLP). Three genotypes (1, 2 and 3) were demonstrable, the most prevalent being HCV type 1 (55 of 100 patients, 55%), followed by HCV type 3 (37 of 100 patients, 37%) and HCV type 2 (8 of 100 patients, 8%). There was an unusual high prevalence of genotype 3, in contrast to the majority of published data from the Southeast region.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil. krug@dna.cbiot.ufrgs.br
authors.forename: L P
authors.initials: LP
authors.lastname: Krug
authors.forename: V R
authors.initials: VR
authors.lastname: Lunge
authors.forename: N
authors.initials: N
authors.lastname: Ikuta
authors.forename: A S
authors.initials: AS
authors.lastname: Fonseca
authors.forename: H
authors.initials: H
authors.lastname: Cheinquer
authors.forename: L S
authors.initials: LS
authors.lastname: Ozaki
authors.forename: S G
authors.initials: SG
authors.lastname: Barros
mesh_terms: D001938:Brazil; D004279:DNA, Viral / Q000032:analysis; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans
chemical_list: D004279:DNA, Viral