title: Oligoadenosine tracts favor nucleosome formation.
abstract: We have measured the ability of oligoadenosine tracts 25 base pairs in length to influence nucleosome formation. Such tracts can cause DNA to bind in nucleosomes at higher temperatures with a free energy up to 1 kcal/mol more favorable than heterogenous-sequence DNA. Furthermore, the position of the oligoadenosine tract affects the free energy of binding, with the most favorable position occurring at the dyad axis.
authors.affiliation: Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
authors.forename: H
authors.initials: H
authors.lastname: Mahloogi
authors.forename: M J
authors.initials: MJ
authors.lastname: Behe
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002151:Calorimetry; D002645:Chickens; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry; D004912:Erythrocytes / Q000648:ultrastructure; D017665:Hydroxyl Radical; D009707:Nucleosomes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D015183:Restriction Mapping; D013816:Thermodynamics
chemical_list: D009707:Nucleosomes; D009838:Oligodeoxyribonucleotides; C030570:oligodeoxyadenylic acid; D017665:Hydroxyl Radical; D004247:DNA