Carbohydrate binding specificity of the neutrophil-activating protein of Helicobacter pylori.
The possible interaction of the neutrophil-activating protein of Helicobacter pylori with target cell glycoconjugates was investigated by the binding of 125I-labeled recombinant protein to glycosphingolipids from human neutrophils in solid phase assays. Thereby, a distinct binding of the neutrophil-activating protein to four bands in the acid glycosphingolipid fraction from human neutrophils was detected, whereas no binding to the non-acid glycosphingolipids or polyglycosyl ceramides from these cells was obtained. When using glycosphingolipids not present in the cell membrane of human neutrophils, it was found that the neutrophil-activating protein also bound to sulfated glycosphingolipids as sulfatide and sulfated gangliotetraosyl ceramide. Comparison of the binding preferences of the protein to reference glycosphingolipids from other sources suggested that in human granulocytes, the neutrophil-activating protein of H. pylori preferentially recognizes glycoconjugates with a terminally unsubstituted NeuAcalpha3Galbeta4GlcNAcbeta3Galbeta4GlcNAcbeta sequence.
Teneberg
S
S
Department of Medical Biochemistry, Göteborg University, Medicinaregatan 9A, S-413 90 Göteborg, Sweden.
Miller-Podraza
H
H
Lampert
H C
HC
Evans
D J
DJ
Evans
D G
DG
Danielsson
D
D
Karlsson
K A
KA
D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D001665:Binding Sites; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D006028:Glycosphingolipids / Q000276:immunology / Q000378:metabolism*; D016480:Helicobacter pylori / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000378:metabolism*; D008969:Molecular Sequence Data; D018375:Neutrophil Activation; D011994:Recombinant Proteins / Q000378:metabolism
D001426:Bacterial Proteins; D006028:Glycosphingolipids; D016209:Interleukin-8; D011994:Recombinant Proteins; C092412:neutrophil-activating protein A, Helicobacter pylori