DNA binding activity of the mammalian translation elongation complex: recognition of chromium- and transplatin-damaged DNA.
The elongation factor complex, EF-1H, serves an essential function in protein biosynthesis in eukaryotic cells, although the role of EF-1H in other physiological processes is unknown. In this report, we demonstrate that three components of EF-1H (EF-1 beta, EF-1 delta, EF-1 gamma) bind to DNA modified with chromium (Cr), a potent DNA-damaging agent and an established human carcinogen. The EF-1H complex also binds to transplatin modified DNA but not to cisplatin-modified DNA. These results demonstrate that the EF-1H complex has functional DNA binding activity and is capable of recognizing the distortions in DNA structure resulting from the covalent binding of Cr and transplatin to DNA.
Wang
J F
JF
Department of Pathology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104-6002, USA.
Engelsberg
B N
BN
Johnson
S W
SW
Witmer
C
C
Merrick
W C
WC
Rozmiarek
H
H
Billings
P C
PC
D000595:Amino Acid Sequence; D000818:Animals; D002277:Carcinoma; D002857:Chromium / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D003432:Cross-Linking Reagents / Q000633:toxicity; D018736:DNA Adducts; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D010051:Ovarian Neoplasms; D010445:Peptide Elongation Factors / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D003432:Cross-Linking Reagents; D018736:DNA Adducts; D004268:DNA-Binding Proteins; D008024:Ligands; D009363:Neoplasm Proteins; D010445:Peptide Elongation Factors; D002857:Chromium; C034697:transplatin; D002945:Cisplatin