Ribosomal DNA difference between species A and D of the Anopheles dirus complex of mosquitoes from China.
Species A and D of the Anopheles dirus complex were found in China. Ribosomal DNA second internal transcribed spacers (ITS2) of both species A and D were sequenced and found to be 716 and 710 base-pairs in length, respectively, with 69% GC content. No evidence of intraspecific variation was detected in the ITS2 sequence of species A, whereas the sequence of species D showed variation at one position in the ITS2. A large number of simple repeat motifs were dispersed throughout the ITS2 sequences. The level of interspecific difference was 5.4% of the nucleotide sequences. Some of the interspecific differences were located in regions with subrepeat structure.
Xu
J N
JN
Department of Parasitology, Second Military Medical University, Shanghai, People's Republic of China.
Qu
F Y
FY
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D002681:China; D004275:DNA, Ribosomal / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D013045:Species Specificity
D004275:DNA, Ribosomal