title: Determination and pharmacokinetics of a furosemide-amiloride drug combination.
abstract: The study presents an accurate and precise HPLC assay for the determination of furosemide and amiloride in human specimens. Both drugs were extracted from human plasma with ethyl acetate; furosemide was extracted at pH 1 and amiloride at pH 12. While chromatographic separation conditions, i.e., column, mobile phase and flow-rate were the same for both investigated drugs, furosemide was detected using a UV absorbance detector, whereas amiloride, because of its very low therapeutic range, was detected with a spectrofluorimetric detector. The linearity of the furosemide and amiloride assays were confirmed over the range of 30-3000 ng/ml and 0.5-30 ng/ml, respectively. These concentrations correspond well with the therapeutic ranges of both drugs. The extraction recoveries, depending on concentration, exceed 80% for furosemide and 74% for amiloride. The reported methods were applied to pharmacokinetic investigations of the two compounds taken in form of a drug combination.
authors.affiliation: Medical Center of Postgraduate Education, Department of Biopharmaceutics, Bydgoszcz, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Jankowski
authors.forename: A
authors.initials: A
authors.lastname: Skorek-Jankowska
authors.forename: H
authors.initials: H
authors.lastname: Lamparczyk
mesh_terms: D000328:Adult; D000584:Amiloride / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004232:Diuretics / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D004338:Drug Combinations; D005665:Furosemide / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004232:Diuretics; D004338:Drug Combinations; D000584:Amiloride; D005665:Furosemide