Direct isolation of human BRCA2 gene by transformation-associated recombination in yeast.
Mutant forms of the BRCA2 gene contribute significantly to hereditary breast cancer. Isolation of the normal and mutant forms of the BRCA2 gene with its natural promoter would greatly facilitate analysis of the gene and its contribution to breast cancer. We have accomplished the direct isolation of the 90-kb gene from total human DNA by transformation-associated recombination in yeast using a small amount of 5' and 3' BRCA2 sequence information. Because the entire isolation procedure of a single chromosomal gene could be accomplished in approximately 2 weeks, the transformation-associated recombination cloning approach is readily applicable to studies of chromosome alterations and human genetic diseases.
Larionov
V
V
Laboratories of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. larionov@niehs.nih.gov
Kouprina
N
N
Solomon
G
G
Barrett
J C
JC
Resnick
M A
MA
D024682:BRCA2 Protein; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae; D014157:Transcription Factors / Q000235:genetics*
D024682:BRCA2 Protein; D009363:Neoplasm Proteins; D014157:Transcription Factors