Apoptosis induced in the spinal cord and dorsal root ganglion by infection of herpes simplex virus type 2 in the mouse.
After inoculation of a highly neuro-invasive strain of herpes simplex virus (HSV) type 2 (186) into the mouse hind-paw planter skin, many virus-positive neurons and glial cells were detected in the dorsal root ganglia (DRGs) and lumbar spinal cord by immunohistochemistry for HSV-2 antigen. A number of apoptotic cells were also observed in the spinal cord and DRGs by the terminal dUTP nick-end-labeling (TUNEL) method. Double labeling with the immunohistochemistry for HSV-2 and the TUNEL method revealed further that some glial and neuronal cells in the spinal cord, either infected or non-infected by HSV-2, showed apoptotic signs. In DRGs, however, apoptosis was detected in no neuronal cells, although some of HSV-2 -infected glial cells were apoptotic.
Ozaki
N
N
Department of Anatomy, Fukushima Medical College, Hikarigaoka, Japan.
Sugiura
Y
Y
Yamamoto
M
M
Yokoya
S
S
Wanaka
A
A
Nishiyama
Y
Y
D000818:Animals; D000914:Antibodies, Viral; D017209:Apoptosis / Q000502:physiology*; D001710:Biotin; D002467:Cell Nucleus / Q000473:pathology / Q000821:virology; D053938:DNA Fragmentation; D003856:Deoxyuracil Nucleotides; D005727:Ganglia, Spinal / Q000166:cytology / Q000821:virology*; D016679:Genome, Viral; D006561:Herpes Simplex / Q000473:pathology*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013116:Spinal Cord / Q000166:cytology / Q000821:virology*; D013194:Staining and Labeling
D000914:Antibodies, Viral; D003856:Deoxyuracil Nucleotides; D001710:Biotin