Molecular analysis of katG gene mutations in strains of Mycobacterium tuberculosis complex from Africa.
A sample of 124 isoniazid (INH)-resistant and 88 susceptible strains of Mycobacterium tuberculosis complex from south, central, and west Africa was analyzed by direct sequence analysis and PCR-restriction fragment length polymorphism analysis of their catalase-peroxidase (katG) genes. Point mutations at codon 315 were found in the genomes of 64% of INH-resistant strains, but no complete deletions were identified. Mutations at codon 463 were independent of INH resistance and were linked to the geographic origins of the strains.
Haas
W H
WH
Department of General Pediatrics, Children's Hospital, Heidelberg University, Germany. ic1@aixterm1.urz.uni-heidelberg.de
Schilke
K
K
Brand
J
J
Amthor
B
B
Weyer
K
K
Fourie
P B
PB
Bretzel
G
G
Sticht-Groh
V
V
Bremer
H J
HJ
D000349:Africa; D002374:Catalase / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D009195:Peroxidase / Q000235:genetics*; D017354:Point Mutation
D002374:Catalase; D009195:Peroxidase