Immunomodulating efficacy of combined administration of galactoside-specific lectin standardized mistletoe extract and sodium selenite in BALB/c-mice.
The immunomodulating abilities of commercially available mistletoe extract standardized for the galactoside-specific lectin (mistletoe lectin-1, ML-1) and sodium selenite (Se) were evaluated in BALB/c-mice and compared to non-treated control animals. Following the optimal schedule of administration (ML-1: 1ng/kg BW; days 1, 2, 3, 5 and Se: 3,5 micrograms/kg BW for 7 subsequent days before evaluation) yielded enhanced counts (thymocytes; peritoneal macrophages, MO; peripheral blood leukocytes; lymphocytes, PBL; monocytes, PBM) and activities (CD-25 positive PBL, MAC-3 positive PBM) of desired immune cells reaching statistical significance for most parameters. However, combined administration of ML-1 and Se proved to be superior to monotherapy since immune cell counts (thymocytes, leukocytes, PBL, PBM) and activities (CD-25 positive PBL) exceeded values obtained after monotherapy. These data are in favour of therapeutical strategies in complementary oncology and suggest that the combination of defined immunomodulating substances might positively enhance the efficacy.
Stoffel
B
B
Institute of Medical Microbiology and Hygiene, University of Köln, Germany.
Krämer
K
K
Mayer
H
H
Beuth
J
J
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis / Q000096:biosynthesis; D004347:Drug Interactions; D005690:Galactose; D007165:Immunosuppression Therapy / Q000379:methods*; D037102:Lectins; D007958:Leukocyte Count / Q000187:drug effects; D018655:Lymphocyte Count / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014784:Mistletoe; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D010946:Plants, Medicinal; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000096:biosynthesis; D054790:Ribosome Inactivating Proteins, Type 2; D018038:Sodium Selenite / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014118:Toxins, Biological / Q000494:pharmacology*
D000943:Antigens, Differentiation; D037102:Lectins; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D015375:Receptors, Interleukin-2; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C036763:monocyte-macrophage differentiation antigen; C009674:ribosome inactivating protein, Viscum; D018038:Sodium Selenite; D005690:Galactose