title: Surreptitious hepatitis C virus (HCV) infection detected in the majority of patients with cryptogenic chronic hepatitis and negative HCV antibody tests.
abstract: Reverse transcription-polymerase chain reaction was used to identify hepatitis C virus (HCV) RNA in peripheral whole blood (WB) and plasma samples from 15 patients with chronic, unexplained hepatitis. These patients were serologically negative for hepatitis A, B, and C and were classified as having chronic non-A, non-B, non-C hepatitis (NANBNC). HCV RNA was repeatedly detected in WB samples from 10 (67%). In contrast, plasma samples from only 5 were intermittently positive. Statistically, HCV RNA detection in WB was significantly more sensitive than in plasma. Nucleic acid hybridization and HCV genotypic analysis confirmed the specificity of the HCV RNA assay. Liver biopsies from these patients suggested histopathologic differences between HCV RNA-positive and -negative groups. These data demonstrate that HCV infection is present in patients with unexplained chronic hepatitis more frequently than previously believed. Additionally, WB HCV RNA detection is more sensitive than plasma assays in identifying antibody-negative HCV infection.
authors.affiliation: Department of Internal Medicine and Pathology, University of Iowa College of Medicine, and VA Medical Center, Iowa City, USA.
authors.forename: W N
authors.initials: WN
authors.lastname: Schmidt
authors.forename: P
authors.initials: P
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Cederna
authors.forename: F A
authors.initials: FA
authors.lastname: Mitros
authors.forename: D R
authors.initials: DR
authors.lastname: LaBrecque
authors.forename: J T
authors.initials: JT
authors.lastname: Stapleton
mesh_terms: D000328:Adult; D000368:Aged; D002908:Chronic Disease; D005260:Female; D006505:Hepatitis / Q000821:virology*; D006526:Hepatitis C / Q000821:virology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D007962:Leukocytes / Q000821:virology; D008099:Liver / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood*
chemical_list: D018937:Hepatitis C Antibodies; D012367:RNA, Viral