title: Nod factors of Azorhizobium caulinodans strain ORS571 can be glycosylated with an arabinosyl group, a fucosyl group, or both.
abstract: In addition to the previously described arabinosylated Nod factors, Azorhizobium caulinodans can also produce fucosylated Nod factors and Nod factors that are both arabinosylated and fucosylated. The presence of a plasmid carrying extra copies of a subset of nod genes as well as bacterial growth conditions influence the relative proportion of carbamoylated, fucosylated, and arabinosylated Nod factors. By using a root hair formation assay, we demonstrate that the Nod factor glycosylations are important for biological activity on Sesbania rostrata roots.
authors.affiliation: Department of Genetics, Flanders Interuniversity, Universiteit Gent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Mergaert
authors.forename: M
authors.initials: M
authors.lastname: Ferro
authors.forename: W
authors.initials: W
authors.lastname: D'Haeze
authors.forename: M
authors.initials: M
authors.lastname: van Montagu
authors.forename: M
authors.initials: M
authors.lastname: Holsters
authors.forename: J C
authors.initials: JC
authors.lastname: Promé
mesh_terms: D001089:Arabinose / Q000378:metabolism; D002240:Carbohydrate Sequence; D007887:Fabaceae / Q000254:growth & development / Q000382:microbiology; D005643:Fucose / Q000378:metabolism; D005798:Genes, Bacterial; D006031:Glycosylation; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D010946:Plants, Medicinal; D012230:Rhizobiaceae / Q000235:genetics / Q000378:metabolism*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D008070:Lipopolysaccharides; D005643:Fucose; D001089:Arabinose