Quantitative evidence that both Hsc70 and Hsp70 contribute to thermal adaptation in hybrids of the livebearing fishes Poeciliopsis.
The 70-kilodalton heat shock protein family is composed of both environmentally inducible (Hsp) and constitutively expressed (Hsc) family members. While the role of the constitutively expressed stress proteins in thermotolerance is largely unknown, de novo expression of stress proteins in response to elevated temperatures has been associated with increased thermotolerance in many cell lines, developing embryos and adult organisms. Distinct, hemiclonal hybrids between the livebearing fish species Poeciliopsis monacha and P. lucida varied in their abilities to survive temperature stress, with survival being greatest when rates of temperature increase to 40 degrees C were slowest and when P. monacha genomes were combined with a sympatric P. lucida genome. Quantification of Hsp70 under heat shock conditions and Hsc70 under normal physiological conditions indicated that variation in survival among hemiclones was best explained by the combined effects of these two proteins. Similar complex interactions between maternal and paternal genomes and rate of temperature increase were found to underlie patterns of survival, Hsp70 accumulation and Hsc70 abundance. These data suggest that the relationship between Hsps and thermotolerance is more intricate than previously thought and that Hsps contribute to thermal adaptation in these fishes through genetic interactions specific to particular environments.
diIorio
P J
PJ
Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs 06269, USA.
Holsinger
K
K
Schultz
R J
RJ
Hightower
L E
LE
D000220:Adaptation, Biological / Q000502:physiology*; D000818:Animals; D005260:Female; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000502:physiology*; D006358:Hot Temperature; D006824:Hybridization, Genetic; D010641:Phenotype; D011036:Poecilia / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D018840:HSP70 Heat-Shock Proteins