title: Simulation of human xenobiotic metabolism in microorganisms. Yeast a good compromise between E. coli and human cells.
abstract: An overview of current heterologous expression systems for xenobiotic metabolising enzymes is given with a special emphasis on the yeast expression system. In a first part, basic properties and relative advantages and drawbacks of each expression system are considered. The second part is dedicated to humanized yeast strains allowing human P450 expression in a tailored redox environment and to the possibility to use such strains to simulate complex metabolisms involving a combination of phase I and phase II reactions. The last part presents how the association of numeric simulation to yeast expression can help in understanding rules controlling metabolic profiles in xenobiotic-acting multienzymatic systems.
authors.affiliation: Centre de Génétique Moléculaire du CNRS, Laboratoire associé à l'Université Pierre-et-Marie-Curie, Gif-sur-Yvette, France.
authors.forename: D
authors.initials: D
authors.lastname: Pompon
authors.forename: J C
authors.initials: JC
authors.lastname: Gautier
authors.forename: A
authors.initials: A
authors.lastname: Perret
authors.forename: G
authors.initials: G
authors.lastname: Truan
authors.forename: P
authors.initials: P
authors.lastname: Urban
mesh_terms: D006801:Humans; D015262:Xenobiotics / Q000378:metabolism*; D015003:Yeasts / Q000378:metabolism*
chemical_list: D015262:Xenobiotics