Clostridium thermocellum JW20 (ATCC 31549) is a coculture with Thermoanaerobacter ethanolicus.
A PCR assay based on 16S rRNA sequence differences among four thermophilic anaerobic bacterial strains was used to demonstrate contamination of Clostridium thermocellum JW20 (ATCC 31549) with a Thermoanaerobacter ethanolicus strain. Therefore, we suggest that interpretation of experimental results with C. thermocellum JW20 be viewed with caution.
Erbeznik
M
M
Department of Animal Sciences, University of Kentucky, Lexington 40546-0215, USA. milerbe@pop.uky.edu
Jones
C R
CR
Dawson
K A
KA
Strobel
H J
HJ
D001421:Bacteria, Anaerobic / Q000235:genetics* / Q000302:isolation & purification*; D003013:Clostridium / Q000235:genetics* / Q000302:isolation & purification*; D018920:Coculture Techniques; D016988:Gram-Positive Asporogenous Rods, Irregular / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D012988:Soil Microbiology
D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S