title: Efficient preparation of single-stranded DNA for in vitro selection.
abstract: In vitro selection of aptamers requires the reliable enzymatic preparation of large amounts of (+) single-stranded DNA molecules. This can be achieved by selective enzymatic digest of 5'-phosphorylated (-) strands from PCR products, a method already widely used in sequencing of PCR products. Here we present an adaptation of this method to prepare large pools of single-stranded DNA molecules for in vitro selection.
authors.affiliation: Hans-Knöll-Institut für Naturstoff-Forschung, Abt.Zell- und Molekularbiologie, Jena, Germany.
authors.forename: M J
authors.initials: MJ
authors.lastname: Kujau
authors.forename: S
authors.initials: S
authors.lastname: Wölfl
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
chemical_list: D004277:DNA, Single-Stranded