[Comparison of localizations of insertions of phage-transposons D3112 and B3 into the Pseudomonas aeruginosa chromosome].
Preferential chromosomal integration sites (sites of conservative transposition) of transposable phage D3112 were identified by means of pulsed-field gel electrophoresis and separation of restriction fragments of the Pseudomonas aeruginosa chromosome. Phase B3 was shown to differ from D3112 with respect to this trait. High site-specificity and regional specificity, typical for conservative transposition of D3112, can partially explain the absence of mutations in biosynthetic P. aeruginosa genes upon cell lysogenization with D3112 phage (unlike B3 phage).
Burkal'steva
M V
MV
Krylov
V N
VN
D002874:Chromosome Mapping / Q000379:methods*; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000235:genetics*; D004586:Electrophoresis; D008242:Lysogeny; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D004279:DNA, Viral