Differential involvement of caspases in apoptosis of myeloid leukemic cells induced by chemotherapy versus growth factor withdrawal.
To assess the potential involvement of the caspase family in the IL-3-dependent murine myeloid leukemic cell line 32D, we studied the effect of bcl-2, crmA and three synthetic caspase inhibitors on apoptosis induced by chemotherapy or IL-3 withdrawal. Apoptosis induced by IL-3 deprivation or by ActD appears to be mediated by a crmA-insensitive pathway. Cell death by IL-3 withdrawal is inhibited by the caspase-inhibitor ZVAD-fmk, but not DEVD-fmk or YVAD-cmk. In contrast, DEVD-fmk as well as ZVAD-fmk protect 32D cells from ActD-induced apoptosis. These results indicate that different caspases are involved in apoptosis induced by growth factor withdrawal and by chemotherapy.
Barge
R M
RM
Laboratory of Molecular Biology, Flanders Interuniversity Institute for Biotechnology, University of Gent, Belgium. Willemze@rullf2.Leidenuniv.nl
Willemze
R
R
Vandenabeele
P
P
Fiers
W
W
Beyaert
R
R
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003546:Cysteine Endopeptidases / Q000378:metabolism / Q000502:physiology*; D015853:Cysteine Proteinase Inhibitors / Q000502:physiology; D003609:Dactinomycin / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D007377:Interleukin-3 / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D051379:Mice; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000502:physiology; D015843:Serpins / Q000096:biosynthesis / Q000502:physiology; D014407:Tumor Cells, Cultured; D014764:Viral Proteins
D015853:Cysteine Proteinase Inhibitors; D007377:Interleukin-3; D019253:Proto-Oncogene Proteins c-bcl-2; D015843:Serpins; D014764:Viral Proteins; D003609:Dactinomycin; C074641:interleukin-1beta-converting enzyme inhibitor; D003546:Cysteine Endopeptidases