title: Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors.
abstract: Malondialdehyde (MDA) is one of the most frequently used indicators of lipid peroxidation. To generate reliable reference intervals for plasma malondialdehyde (P-MDA), a reference sample group was established in Funen, Denmark. The group consisted of 213 individuals (107 men, 106 women), ages 20-79 years. P-MDA was measured in EDTA-treated plasma after derivatization by thiobarbituric acid (TBA) and separation on HPLC. UV detection was performed at 532 nm. A reference interval was calculated as recommended by IFCC with REFVAL 3.42. The estimated reference limits (0.025 and 0.975 fractals) for the group were 0.36 and 1.24 mumol/L. The data were analyzed for gender- and age-related differences. Analysis of variance showed no interaction between gender and age, but separate analyses showed an independent effect of gender (P = 0.03), but not of age (P = 0.11). Daily smokers had a slightly higher average concentration of P-MDA than nonsmokers (P = 0.05), and P-MDA correlated with daily exposure to cigarette smoke (r = 0.162; P = 0.03). A positive correlation was also demonstrated between P-MDA and weekly alcohol consumption (r = 0.153; P = 0.03). Within-subject and day-to-day variations of P-MDA indicated that the potential of P-MDA as a biomarker for individuals is questionable. However, on a group basis, the present data support that P-MDA may be a potential biomarker for oxidative stress.
authors.affiliation: Institute of Community Health, Odense University, Denmark. F.Nielsen@Winsloew.ou.dk
authors.forename: F
authors.initials: F
authors.lastname: Nielsen
authors.forename: B B
authors.initials: BB
authors.lastname: Mikkelsen
authors.forename: J B
authors.initials: JB
authors.lastname: Nielsen
authors.forename: H R
authors.initials: HR
authors.lastname: Andersen
authors.forename: P
authors.initials: P
authors.lastname: Grandjean
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging; D000428:Alcohol Drinking; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D003718:Denmark; D005260:Female; D006801:Humans; D008019:Life Style; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D018384:Oxidative Stress; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012727:Sex Characteristics; D012907:Smoking / Q000097:blood; D013858:Thiobarbiturates
chemical_list: D015415:Biomarkers; D013858:Thiobarbiturates; D008315:Malondialdehyde; C029684:thiobarbituric acid