title: Expression of estrogen receptor-beta in human breast tumors.
abstract: The expression of a recently described novel estrogen receptor, ER-beta, was detected in several human breast tumor biopsy samples and several human breast epithelial cell lines using reverse transcription and polymerase chain reaction (RT-PCR) analysis. Cloning and sequencing of the PCR product from a breast tumor confirmed the identity of the sequence with that of the ER-beta mRNA previously reported in human testis. The expression of ER-beta was not correlated with that of ER-alpha, and both ER-alpha positive and ER-alpha negative cell lines expressed ER-beta mRNA. However, some breast tumors and some cell lines coexpress ER-beta and ER-alpha mRNA. Our data support a possible role for ER-beta in human breast cancer.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Dotzlaw
authors.forename: E
authors.initials: E
authors.lastname: Leygue
authors.forename: P H
authors.initials: PH
authors.lastname: Watson
authors.forename: L C
authors.initials: LC
authors.lastname: Murphy
mesh_terms: D001706:Biopsy; D001943:Breast Neoplasms / Q000378:metabolism*; D002478:Cells, Cultured; D047629:Estrogen Receptor beta; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D011960:Receptors, Estrogen