STRL22 is a receptor for the CC chemokine MIP-3alpha.
STRL22 is a human seven transmembrane domain orphan receptor related to known chemokine receptors and expressed in peripheral blood lymphocytes, tumor infiltrating lymphocytes and lymphoid tissues. MIP-3alpha/LARC/Exodus is a CC chemokine that is chemotactic for lymphocytes and that is expressed in activated cells, including monocytes, T cells, endothelial cells, and fibroblasts, and in liver, lung, and some lymphoid tissues. We report here that STRL22-transfected human embryonic kidney 293 cells demonstrated specific binding for MIP-3alpha and that MIP-3alpha, but no other chemokines, produced a calcium flux in the STRL22-transfected cells. We show that MIP-3alpha, unlike other chemokines, produced a calcium flux in freshly-isolated peripheral blood lymphocytes and we show that MIP-3alpha also produced a signal in tumor infiltrating lymphocytes that express STRL22. Since STRL22 is the sixth functional CC chemokine receptor identified, it should be re-named CCR6.
Liao
F
F
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Alderson
R
R
Su
J
J
Ullrich
S J
SJ
Kreider
B L
BL
Farber
J M
JM
D002460:Cell Line; D054418:Chemokine CCL20; D018925:Chemokines / Q000378:metabolism; D019742:Chemokines, CC; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D019402:Macrophage Inflammatory Proteins; D008969:Molecular Sequence Data; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*
C108355:CCL20 protein, human; C106311:CCR6 protein, human; D054418:Chemokine CCL20; D018925:Chemokines; D019742:Chemokines, CC; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D054399:Receptors, CCR6; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine