Characterization of the avian Ich-1 cDNA and expression of Ich-1L mRNA in the hen ovary.
We have sequenced the chicken interleukin-1beta-converting enzyme (ICE) and ced-3 homolog (Ich-1) cDNA, and evaluated Ich-1 mRNA expression in the hen ovary during follicle development. While two alternatively spliced forms of Ich-1, Ich-1L and Ich-1S, were amplified by PCR from an embryonic chicken cDNA library, only the Ich-1L form was found to be expressed in adult ovarian granulosa and theca tissues. The deduced amino acid (aa) sequence of ICH-1L is 70.8% identical to human ICH-1L and contains the conserved QACRG peptide active catalytic sequence characteristic of many ICE-related family of cysteine proteases.
Johnson
A L
AL
Department of Biological Sciences, The University of Notre Dame, IN 46556, USA. johnson.128@nd.edu
Bridgham
J T
JT
Bergeron
L
L
Yuan
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D053143:Caspase 2; D020169:Caspases; D002645:Chickens; D003546:Cysteine Endopeptidases / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D008969:Molecular Sequence Data; D010053:Ovary / Q000502:physiology; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; D053143:Caspase 2; D020169:Caspases; D003546:Cysteine Endopeptidases