Dual targets of a transcriptional activator that tracks on DNA.
The sliding clamp of the bacteriophage T4 DNA polymerase, gp45, is also the proximal effector for activation of transcription of T4 late genes. We have identified the phage T4-encoded sigma factor gp55 and the co-activator gp33 as targets of gp45 in promoter complexes, and have shown that a conserved carboxy-terminal amino acid sequence of gp55 and gp33 is required for interaction with gp45. The respective contribution of each target-gp45 interaction to activation of transcription has been assessed by measuring promoter opening rates. The opening rate supported by interaction with both targets is far greater than the arithmetical sum of the separate contributions of each target, implying a synergistic activation of transcription through at least two separate interactions of the trimeric gp45.
Sanders
G M
GM
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093-0634, USA.
Kassavetis
G A
GA
Geiduschek
E P
EP
D017122:Bacteriophage T4 / Q000201:enzymology / Q000235:genetics*; D001665:Binding Sites; D003432:Cross-Linking Reagents; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D010777:Photochemistry; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012808:Sigma Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism
D003432:Cross-Linking Reagents; D012808:Sigma Factor; D014157:Transcription Factors; D014764:Viral Proteins; C085859:gene 33 protein, Enterobacteria phage T4; C069983:gene 43 protein, Enterobacteria phage T4; C106892:gene 55 protein, Enterobacteria phage T4; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase