title: Efficiency of the polymerase chain reaction amplification of the uid gene for detection of Escherichia coli in contaminated water.
abstract: Direct detection of Escherichia coli from polluted river water was achieved using polymerase chain reaction (PCR) amplification of the uid gene. Amplification using DNA from environmental samples resulted in non-specific DNA fragments. Specific amplification was achieved through use of the touch-down PCR procedure. Targeting the uidA structural region of the gene gave reproducibly better amplification than targeting the uidR regulatory region. The data demonstrate conditions for optimal specific detection.
authors.affiliation: Department of Genetics and Microbiology, University of Liverpool, UK.
authors.forename: S
authors.initials: S
authors.lastname: Iqbal
authors.forename: J
authors.initials: J
authors.lastname: Robinson
authors.forename: D
authors.initials: D
authors.lastname: Deere
authors.forename: J R
authors.initials: JR
authors.lastname: Saunders
authors.forename: C
authors.initials: C
authors.lastname: Edwards
authors.forename: J
authors.initials: J
authors.lastname: Porter
mesh_terms: D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005966:Glucuronidase / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012722:Sewage / Q000382:microbiology; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012722:Sewage; D005966:Glucuronidase