title: Effect of oncogene transformation of rat embryo cells on cellular oxygen consumption and glycolysis.
abstract: We found an unique effect of oncogene transfections on rat embryo cell (REF) respiration, glycolysis and radiation response. Radioresistance, defined as an increase in Do, increases for REF cells transfected with v-myc or H-ras oncogenes. The combination of both oncogenes confers the maximal radioresistance. Our work shows inhibition of oxygen uptake when cells are transfected with v-myc or H-ras alone. However, oxygen uptake increases when cells are transfected simultaneously with v-myc + H-ras (3.7,2.1,2.8). A higher oxygen consumption results from increased utilization of pyruvate via the Kreb's cycle. Succinate stimulates cellular oxygen consumption. The maximum stimulation of oxygen consumption by succinate occurred with v-myc + H-ras transfected cells. The glycolysis of the transfected cells is also altered by the oncogenes. Our glycolytic measurements indicate the H-ras oncogene causes the largest stimulation of glycolysis. Our data shows that transfection with oncogenes has a major effect on cellular glycolysis, oxidative metabolism as well as the subsequent radiation response.
authors.affiliation: Department of Radiation Oncology, and Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Biaglow
authors.forename: G
authors.initials: G
authors.lastname: Cerniglia
authors.forename: S
authors.initials: S
authors.lastname: Tuttle
authors.forename: V
authors.initials: V
authors.lastname: Bakanauskas
authors.forename: C
authors.initials: C
authors.lastname: Stevens
authors.forename: G
authors.initials: G
authors.lastname: McKenna
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002952:Citric Acid Cycle; D004622:Embryo, Mammalian; D016259:Genes, myc; D011905:Genes, ras; D006019:Glycolysis; D007700:Kinetics; D010101:Oxygen Consumption; D051381:Rats; D013386:Succinates / Q000378:metabolism; D014162:Transfection
chemical_list: D013386:Succinates