An ADP-ribosylation-factor(ARF)-like protein involved in regulated secretion.
A rat ADP-ribosylation factor(ARF)-like protein named ARL184 was identified by cDNA cloning. The corresponding recombinant protein had an apparent molecular mass of 22,000. The deduced amino acid sequence had 55% identity with the human ARL1 and four functional GTP-binding sites. Immunofluorescent confocal microscopy studies showed that ARL184 was present in the cytosol as well as in the Golgi apparatus, raising the possibility that it has a role in a secretory pathway. The involvement of this ARF-like protein in secretion was confirmed by demonstrating that ARL184 potentiated acetylcholine release in stably transfected PC12 cells. Collectively these results suggest that this ARL protein is a component of a regulated secretory pathway involved in Ca2(+)-dependent release of acetylcholine.
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Laboratoire de Génétique Moléculaire de la Neurotransmission, C.N.R.S., Hôpital de la Pitié-Salpêtrière, Paris, France.
Ravassard
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Liepkalns
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Chatail
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D020727:ADP-Ribosylation Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D016716:PC12 Cells; D051381:Rats; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C485820:Arl2 protein, rat; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors