title: Acute action of choriogonadotropin on Leydig tumor cells: qualitative and quantitative transformation of lipid moieties.
abstract: Testicular Leydig cells use either exogenous or de novo synthesized cholesterol as the substrate for the production of testosterone with hormone stimulation. Although the long-term effect of trophic hormones on Leydig cell cholesterol uptake, storage, and deesterification has been well documented, the early effects of the human choriogonadotropin (hCG) on cell cholesterol/lipid distribution are not yet known.
  
  
  
  Sections of cells treated with hCG for 15 sec to 30 min were examined by electron microscopy (EM) for the surface density of lipid moieties in the cytoplasm. In addition, the time-dependent distribution of lipids within the cytoplasmic inclusions and the ultimate destination of this substrate were evaluated by EM. The results were analyzed with standard morphometric methods.
  
  
  
  The surface density of cytoplasmic lipid pools increased significantly within the 15 sec following the exposure of cells to hCG, and it tapered off to the control level in the subsequent 30 min. Such a fluctuation in the amount of cytoplasmic lipids may be due to (1) the quantity of released substrate from the reticular compartment or (2) the rate of its transport across the outer mitochondrial membrane to the inner mitochondrial cytochrome P450 side-chain cleavage, where steroidogenesis begins with the conversion of cholesterol to pregnenolone. These two processes were not quantitatively coordinated in the stimulated cell during the initial 30 min, resulting in a surplus of cytoplasmic lipid pools. To compensate for such uneven metabolic balance, the cell apparently disposed of the excess substrate by a mechanism of molecular regrouping from a micellar configuration to a bilayer structure followed by exocytosis.
authors.affiliation: Department of Cell Biology, Georgetown University Medical Center, Washington, D.C. 20007, USA.
authors.forename: B
authors.initials: B
authors.lastname: Vidic
authors.forename: N
authors.initials: N
authors.lastname: Boujrad
authors.forename: V
authors.initials: V
authors.lastname: Papadopoulos
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D006801:Humans; D002479:Inclusion Bodies / Q000187:drug effects / Q000648:ultrastructure; D007984:Leydig Cell Tumor / Q000378:metabolism* / Q000648:ultrastructure; D050356:Lipid Metabolism; D051379:Mice; D008854:Microscopy, Electron; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D006063:Chorionic Gonadotropin