title: Characterization of cholecystokininA and cholecystokininB receptors expressed by vagal afferent neurons.
abstract: The cholecystokinin receptors expressed by vagal afferent neurons mediate the effect of cholecystokinin in inhibiting food intake and gastric emptying. We have determined the relative abundance of cholecystokininA, gastrin-cholecystokininB and gastrin-cholecystokininC receptor populations in the rat vagus by autoradiography using [125I]Bolton Hunter-cholecystokinin-8, [125I]Bolton Hunter-heptadecapeptide gastrin and [125I]Leu(15)2-17Glycine-extended heptadecapeptide gastrin, together with the selective antagonists devazepide and L-740093. The results indicate approximately three-fold higher abundance of cholecystokininA compared with gastrin-cholecystokininB receptors, and no significant representation of gastrin-cholecystokininC receptors. Topical capsaicin applied to the vagal nerve trunk abolished the accumulation of sites binding both [125I]Bolton Hunter-labelled cholecystokinin-8 and heptadecapeptide gastrin indicating that both cholecystokininA and gastrin-cholecystokininB receptor populations were present on afferent fibres. The molecular identity of the receptors expressed by rat and human nodose ganglia was examined using the reverse transcription polymerase chain reaction. Products of the predicted size for the cholecystokininA and gastrin-cholecystokininB receptors were identified. The human and rat cholecystokininA receptor products were cloned and the sequences were found to be 99% homologous to those published for receptors expressed by rat pancreas and human gall bladder. We conclude that cholecystokininA and gastrin-colecystokininB receptors are synthesized by nodose ganglion cells, and that the receptor proteins are transported to the periphery along afferent fibres. While there is a clear role for vagal cholecystokininA receptors, the function of vagal afferent gastrin-cholecystokininB receptors remains to be determined.
authors.affiliation: Physiological Laboratory, University of Liverpool, U.K.
authors.forename: P
authors.initials: P
authors.lastname: Moriarty
authors.forename: R
authors.initials: R
authors.lastname: Dimaline
authors.forename: D G
authors.initials: DG
authors.lastname: Thompson
authors.forename: G J
authors.initials: GJ
authors.lastname: Dockray
mesh_terms: D000344:Afferent Pathways / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000187:drug effects / Q000378:metabolism*; D014630:Vagus Nerve / Q000378:metabolism*
chemical_list: D011949:Receptors, Cholecystokinin