Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses.
CCR5 and CXCR4 are the two major coreceptors that have been identified for human immunodeficiency virus (HIV) entry. We have modified several beta-galactosidase-based HIV indicator cell lines to express CCR5 and/or CXCR4. Using these new reagents, we have been able to detect all primary isolates tested using one or both of these cell lines. However, there is large variation in the absolute viral infectivity among primary strains. Furthermore, all HIV strains are capable of causing syncytia in the indicator cells when the coreceptor is present regardless of whether they had previously been characterized as "syncytia-inducing" or "non-syncytium-inducing."
Vodicka
M A
MA
Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Goh
W C
WC
Wu
L I
LI
Rogel
M E
ME
Bartz
S R
SR
Schweickart
V L
VL
Raport
C J
CJ
Emerman
M
M
D000818:Animals; D002460:Cell Line; D015726:Giant Cells; D015497:HIV-1 / Q000302:isolation & purification* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism*; D015728:Receptors, HIV / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000302:isolation & purification* / Q000378:metabolism
D008565:Membrane Proteins; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D018121:Receptors, Cytokine; D015728:Receptors, HIV; D011993:Recombinant Fusion Proteins