Protective effect of interleukin-1 receptor antagonist on oleic acid-induced lung injury.
OBJECTIVE
To observe the changes of interleukin-1 (IL-1), nitric oxide (NO) and nitric oxide synthase (NOS) in mice with oleic acid-induced acute lung injury (ALI) and the protective effects of interleukin-1 receptor antagonist (IL-1ra).


METHODS
Male Kunming mice were divided into control, oleic acid and IL-1ra groups. The control group mice were injected saline; the oleic acid group mice were injected oleic acid (0.2 ml/kg): and the IL-1ra group mice were injected the IL-1ra (20 mg/kg). Lung index, lung wet-to-dry weight ratio, and total protein, cell analysis, nitric oxide measurement, NOS activity in BALF, lung pathology examination were made after an hour of administration of drug.


RESULTS
Preadministration of IL-1ra to the mouse with ALI decreased the lung index, lung wet-to-dry weight ratio and leakage of protein from pulmonary capillary, elevated PaO2, and attenuated lung histologic injury. It was found that in bronchoalveolar lavage fluid (BALF), NO amount and lung NOS activity increased in oleic acid group, BALF NO amount and lung NOS activity decreased obviously after given IL-1ra.


CONCLUSIONS
This study demonstrated the protective effect of IL-1ra on oleic acid-induced lung injury, NO may participate in the pathological process of lung injury.
Sun
D
D
Department of Respiratory Diseases, Third Affiliated Hospital, Beijing Medical University.
Zhao
M
M
Ma
D
D
Liao
S
S
Di
C
C
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000378:metabolism; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D019301:Oleic Acid; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000517:prevention & control*; D012795:Sialoglycoproteins / Q000494:pharmacology*
C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D019301:Oleic Acid; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase