Optimizing multiplex and LA-PCR with betaine.
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and 'long and accurate' polymerase chain reaction (LA-PCR). For details on how to partake in the newsgroup, see the accompanying box.
Hengen
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PN
National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702-1201, USA. pnh@ncifcrf.gov
D016477:Artifacts; D001483:Base Sequence; D001622:Betaine; D003195:Computer Communication Networks; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008899:Mineral Oil; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods; D019914:Taq Polymerase
D017931:DNA Primers; D001622:Betaine; D008899:Mineral Oil; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase