Quantitation of cocaine and cocaethylene in small volumes of rat whole blood using gas chromatography-mass spectrometry.
A simple solid phase extraction (SPE) technique combined with gas chromatography-mass spectrometry (GC/MS) operated in selected ion monitoring (SIM) mode is described for quantitation of cocaine and cocaethylene in small samples (250 microliters) of rat whole blood. Use of (N-[2H3C])-cocaine and (N-[2H3C])-cocaethylene internal standards resulted in high sensitivity and selectivity for this analytical method. Analysis was performed using a Hewlett-Packard 5890 GC equipped with a 7673A Automatic Liquid Sampler linked to a Hewlett-Packard 5972 Mass Selective Detector. Separation of analytes was accomplished on a cross-linked methyl silicone gum capillary column (Ultra 1: 12m x 0.2mm (i.d.) x 0.33 microns). Linearity was established over a wide range of concentrations (5.0-2000.0 ng ml-1) with good precision. Limits of detection (LOD) were 1.0 and 2.0 ng ml-1 for cocaine and cocaethylene, respectively. This analytical method was designed for use in pharmacokinetic experiments studying the formation of cocaethylene following ethanol pretreatment in rats administered cocaine.
Burdick
J D
JD
Department of Pharmacology/Toxicology Graduate School of Pharmaceutical Sciences, Duquesne University, Pittsburgh, PA 15282, USA.
Boni
R L
RL
Fochtman
F W
FW
D000818:Animals; D003042:Cocaine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D004347:Drug Interactions; D000431:Ethanol / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
D000431:Ethanol; C066444:cocaethylene; D003042:Cocaine