Cloning and characterization of a novel member of the human Mad gene family (MADH6).
MAD (mothers against decapentaplegic)-related proteins (MADRs) are intracellular components that play critical roles in signal-transduction pathways involving the transforming growth factor beta (TGFbeta) superfamily. Some Mad genes are candidates for tumor-suppressor functions. From a human fetal brain cDNA library we have isolated a novel Mad-related gene. Two alternatively transcribed mRNAs encode deduced 430- and 467-amino-acid peptides that showed high levels of similarity to MADR1/Smad1/hMAD1 (about 80% identity at the amino acid level). This gene, which we designated MADH6, resides on 13q12-q14 between BRCA2 and RB, a region that frequently displays loss of heterozygosity in breast, liver, and prostate cancers.
Watanabe
T K
TK
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Kawauchi-cho, Tokushima, Japan. watanabe@otsuka.genome.ad.jp
Suzuki
M
M
Omori
Y
Y
Hishigaki
H
H
Horie
M
M
Kanemoto
N
N
Fujiwara
T
T
Nakamura
Y
Y
Takahashi
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002882:Chromosomes, Human, Pair 13; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides; D011506:Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D051904:Smad8 Protein; D014018:Tissue Distribution; D014157:Transcription Factors; D016212:Transforming Growth Factor beta / Q000235:genetics*
D018076:DNA, Complementary; D010455:Peptides; D011506:Proteins; C107514:SMAD9 protein, human; D051904:Smad8 Protein; D014157:Transcription Factors; D016212:Transforming Growth Factor beta