Expression of functional gap junctions in cultured pulmonary alveolar epithelial cells.
Recent observations suggest that cell-cell interactions may modulate the response of the alveolar epithelium to injury. Expression and function of gap junctions were thus evaluated in isolated alveolar type II cells. Freshly isolated (day 0) type II cells expressed mRNAs for gap junctional connexins 26, 32, and 43. Whereas connexin 26 mRNA declined approximately 40% in cultured cells, connexin 32 message decreased rapidly and was not detectable on day 1. In contrast, connexin 43 expression increased 10-fold by day 3 compared with day 0. Western blot confirmed a 30-fold elevation in connexin 43 protein. Connexin 45 mRNA was not detected. Functional gap junction-mediated calcium signal propagation was monitored using fura 2, a calcium-sensitive dye. Membrane deformation in a single type II cell increased intracellular calcium; the signal spread rapidly to neighboring cells by octanol-sensitive pathways. Transfer of Lucifer yellow between cells also was inhibited by octanol. These observations demonstrate functional gap junctions between cultured alveolar epithelial cells and suggest that gap junctional expression and gap junction-mediated cell-cell coupling are regulated with time in culture.
Lee
Y C
YC
Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, hershey 17033, USA.
Yellowley
C E
CE
Li
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Z
Donahue
H J
HJ
Rannels
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DE
D000818:Animals; D015153:Blotting, Western; D002450:Cell Communication; D002478:Cells, Cultured; D000072259:Connexin 26; D018031:Connexin 43 / Q000096:biosynthesis*; D017630:Connexins / Q000096:biosynthesis*; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005456:Fluorescent Dyes; D017629:Gap Junctions / Q000502:physiology*; D007546:Isoquinolines; D007700:Kinetics; D008297:Male; D011650:Pulmonary Alveoli / Q000166:cytology / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014158:Transcription, Genetic; D000097002:Gap Junction beta-1 Protein
D000072259:Connexin 26; D018031:Connexin 43; D017630:Connexins; D005456:Fluorescent Dyes; D007546:Isoquinolines; D012333:RNA, Messenger; D000097002:Gap Junction beta-1 Protein; C000607044:Gjb2 protein, rat; C017475:lucifer yellow