title: Multiplex fluorescence-based primer extension method for quantitative mutation analysis of mitochondrial DNA and its diagnostic application for Alzheimer's disease.
abstract: A sensitive and highly reproducible multiplexed primer extension assay is described for quantitative mutation analysis of heterogeneous DNA populations. Wild-type and mutant target DNA are simultaneously probed in competitive primer extension reactions using fluorophor-labeled primers and high fidelity, thermostable DNA polymerases in the presence of defined mixtures of deoxy- and dideoxynucleotides. Primers are differentially extended and the resulting products are distinguished by size and dye label. Wild-type:mutant DNA ratios are determined from the fluorescence intensities associated with electrophoretically resolved reaction products. Multiple nucleotide sites can be simultaneously interrogated with uniquely labeled primers of different lengths. The application of this quantitative technique is shown in the analysis of heteroplasmic point mutations in mitochondrial DNA that are associated with Alzheimer's disease.
authors.affiliation: MitoKor, 11494 Sorrento Valley Road, San Diego, CA 92121, USA.
authors.forename: E
authors.initials: E
authors.lastname: Fahy
authors.forename: R
authors.initials: R
authors.lastname: Nazarbaghi
authors.forename: M
authors.initials: M
authors.lastname: Zomorrodi
authors.forename: C
authors.initials: C
authors.lastname: Herrnstadt
authors.forename: W D
authors.initials: WD
authors.lastname: Parker
authors.forename: R E
authors.initials: RE
authors.lastname: Davis
authors.forename: S S
authors.initials: SS
authors.lastname: Ghosh
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis* / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial; D005452:Fluoresceins / Q000737:chemistry*; D006801:Humans
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D005452:Fluoresceins