When does the lung die? Kfc, cell viability, and adenine nucleotide changes in the circulation-arrested rat lung.
Lungs harvested from cadaveric circulation-arrested donors may increase the donor pool for lung transplantation. To determine the degree and time course of ischemia-reperfusion injury, we evaluated the effect of O2 ventilation on capillary permeability [capillary filtration coefficient (Kfc)], cell viability, and total adenine nucleotide (TAN) levels in in situ circulation-arrested rat lungs. Kfc increased with increasing postmortem ischemic time (r = 0.88). Lungs ventilated with O2 1 h postmortem had similar Kfc and wet-to-dry ratios as controls. Nonventilated lungs had threefold (P < 0.05) and sevenfold (P < 0.0001) increases in Kfc at 30 and 60 min postmortem compared with controls. Cell viability decreased in all groups except for 30-min postmortem O2-ventilated lungs. TAN levels decreased with increasing ischemic time, particularly in nonventilated lungs. Loss of adenine nucleotides correlated with increasing Kfc values (r = 0.76). This study indicates that lungs retrieved 1 h postmortem may have normal Kfc with preharvest O2 ventilation. The relationship between Kfc and TAN suggests that vascular permeability may be related to lung TAN levels.
Jones
D R
DR
Department of Surgery, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA.
Becker
R M
RM
Hoffmann
S C
SC
Lemasters
J J
JJ
Egan
T M
TM
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival / Q000502:physiology; D003643:Death; D006323:Heart Arrest / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D006439:Hemodynamics / Q000502:physiology; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D009929:Organ Size / Q000502:physiology; D010101:Oxygen Consumption / Q000502:physiology; D011180:Postmortem Changes; D011652:Pulmonary Circulation / Q000502:physiology; D011669:Pulmonary Wedge Pressure / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology
D000227:Adenine Nucleotides