Study of the delivery of the gD gene of pseudorabies virus to one-day-old piglets by adenovirus or plasmid DNA as ways to by-pass the inhibition of immune response by colostral antibodies.
In the present study, it was shown that piglets with maternal antibodies, which had been primed with a replication-defective adenovirus that expresses the pseudorabies virus (PRV) glycoprotein gD and boosted with the Bartha vaccine strain at 10 weeks of age are equally protected clinically upon a challenge as piglets without maternal antibodies vaccinated with the same approach or with the Bartha vaccine strain alone. Priming with a plasmid that expresses gD was less efficient.
Le Potier
M F
MF
Centre National d'Etudes Vétérinaire et Alimentaire, UR virologie et immunologie porcine, Ploufragan, France.
Monteil
M
M
Houdayer
C
C
Eloit
M
M
D000256:Adenoviridae; D000818:Animals; D000831:Animals, Newborn; D003126:Colostrum / Q000276:immunology*; D003673:Defective Viruses; D005260:Female; D018014:Gene Transfer Techniques; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D007112:Immunity, Maternally-Acquired; D011247:Pregnancy; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D022421:Pseudorabies Vaccines; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D013552:Swine; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines
D022421:Pseudorabies Vaccines; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C107752:glycoprotein D, pseudorabies virus