title: Function and expression of a human idiotypic network in Schistosomiasis japonica.
abstract: The cross-reactive idiotype (Hu-SJ-CRIM) is defined by polyclonal human anti-idiotypic antibodies derived from chronically S. japonicum infected patients. The present study shows that serum levels of Hu-SJ-CRIM expressed by antibodies to S. japonicum soluble egg antigen (SEA) are associated with acute infection and hepatosplenic disease. Xenogeneic anti-idiotypic antisera (anti-Hu-SJ-CRIM) suppressed human lymphocyte blastogenesis to SEA in vitro by 47-82% (P < 0.05). These anti-idiotypic antibodies also suppressed in vitro granuloma formation induced by SEA coated heads in a dose dependent manner. This immunosuppression was antigen specific in that mitogen (PHA) or non-related antigen (PPD) induced blastogenic responses were not suppressed. Surprisingly, anti-idiotypic antibodies (anti-SJ-CRIM), which describe the mouse correlate CRIM were not suppressive in the human blastogenesis or in vitro granuloma formation assays. These data indicate a dichotomy in the function and specificity of the idiotype/anti-idiotype human and murine immune networks in S. japonicum infection. Thus, only the patient derived molecules and serology form the basis for an immunoregulatory network in Schistosomiasis japonica.
authors.affiliation: Department of Medicine, New England Deaconess Hospital, Boston MA, USA.
authors.forename: A V
authors.initials: AV
authors.lastname: Wisnewski
authors.forename: G R
authors.initials: GR
authors.lastname: Olds
authors.forename: J H
authors.initials: JH
authors.lastname: Johnson
authors.forename: B
authors.initials: B
authors.lastname: Ramirez
authors.forename: T F
authors.initials: TF
authors.lastname: Kresina
mesh_terms: D000328:Adult; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D000909:Antibodies, Helminth / Q000097:blood; D000910:Antibodies, Heterophile / Q000097:blood; D000947:Antigens, Helminth; D002648:Child; D005260:Female; D006099:Granuloma / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007130:Immunoglobulin Idiotypes / Q000097:blood*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D010063:Ovum / Q000276:immunology; D011247:Pregnancy; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology*; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000909:Antibodies, Helminth; D000910:Antibodies, Heterophile; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes