Molecular cloning of cDNA for lysenin, a novel protein in the earthworm Eisenia foetida that causes contraction of rat vascular smooth muscle.
Lysenin, which causes contraction of rat vascular smooth muscle, is a protein that was isolated from the earthworm Eisenia foetida. A cDNA encoding lysenin was isolated by use of a partial cDNA probe that had been generated by the PCR with a primer designed by reference to an internal peptide sequence of lysenin. This clone had an ORF encoding 297 amino acid residues. The amino acid sequence deduced from the cDNA revealed the absence of any significant homology to those of previously characterized vasoactive substances. The recombinant lysenin was produced in Escherichia coli. This protein and native lysenin isolated from the earthworm had similar contractive activities when tested on rat aorta. Northern blot analysis of the RNA from various tissues of the earthworm indicated that lysenin is produced by the coelomocytes.
Sekizawa
Y
Y
Research Laboratory, Zenyaku Kogyo Co., Ltd., Nerima-ku, Tokyo, Japan.
Kubo
T
T
Kobayashi
H
H
Nakajima
T
T
Natori
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009835:Oligochaeta / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics* / Q000494:pharmacology; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D014118:Toxins, Biological; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D018076:DNA, Complementary; D011506:Proteins; D011994:Recombinant Proteins; D014118:Toxins, Biological; D014662:Vasoconstrictor Agents; C107040:lysenin