Function of P-glycoprotein expressed in placenta and mole.
We examined the expression of P-glycoprotein in human placentas and hydatidiform moles. Trophoblasts in all the examined placentas and moles expressed P-glycoprotein, and the size of the P-glycoprotein was smaller than that in multidrug-resistant human epidermoid carcinoma KB cells. The P-glycoprotein in the placenta and mole was photolabeled with [3H]azidopine, and [3H]vincristine was transported in an ATP-dependent manner into membrane vesicles prepared from trophoblasts that expressed P-glycoprotein. These findings indicate that P-glycoprotein expressed in trophoblasts has a drug binding site(s) and the ability to transport vincristine, suggesting that P-glycoprotein in the placenta protects the fetus from xenobiotics and confers drug resistance on moles.
Nakamura
Y
Y
The Institute for Cancer Research and Department of Obstetrics and Gynecology, Faculty of Medicine, Kagoshima University, Sakuragaoka, Japan.
Ikeda
S
S
Furukawa
T
T
Sumizawa
T
T
Tani
A
A
Akiyama
S
S
Nagata
Y
Y
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis / Q000096:biosynthesis*; D000255:Adenosine Triphosphate / Q000378:metabolism; D001386:Azides / Q000378:metabolism; D001692:Biological Transport; D004095:Dihydropyridines / Q000378:metabolism; D018432:Drug Resistance, Multiple; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000378:metabolism*; D007150:Immunohistochemistry; D007624:KB Cells; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000378:metabolism*; D014750:Vincristine / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001386:Azides; D004095:Dihydropyridines; D014750:Vincristine; C041130:azidopine; D000255:Adenosine Triphosphate