Fumaric acid, anti-thrombin substance from Rhizopus javanicus.
An anti-thrombin substance (M2) was isolated from a culture broth of Rhizopus javanicus. Accumulation of M2 reached a maximum peak after 14 to 15 days of incubation and then decreased. The yield of M2 was 500 mg from 1 L of culture broth. M2 inhibited thrombin activity, and its 50% inhibition concentration in a reaction mixture containing 50 microL of 12.5 NIH unit/mL thrombin and 200 microL of 0.33% bovine fibrinogen was 63 microM. M2 had a specific activity for thrombin, but it was less responsive to plasmin, tissue-type plasminogen activator, urokinase, plasma kallikrein and glandular kallikrein. The structure of M2 was identified as fumaric acid by elementary analysis, FAB/MS, 1H-NMR, 13C-NMR and IR spectra.
Okamura
T
T
Department of Food Science and Nutrition, School of Home Life and Environmental Science, Mukogawa Women's University, Nishinomiya, Japan.
Horie
N
N
Miyazaki
Y
Y
Ohsugi
M
M
D000818:Animals; D000991:Antithrombins / Q000302:isolation & purification* / Q000494:pharmacology; D001780:Blood Coagulation Tests; D002417:Cattle; D003470:Culture Media; D005650:Fumarates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012233:Rhizopus / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
D000991:Antithrombins; D003470:Culture Media; D005650:Fumarates; C032005:fumaric acid