title: Bovine immunodeficiency virus tat gene: cloning of two distinct cDNAs and identification, characterization, and immunolocalization of the tat gene products.
abstract: cDNAs encoding the bovine immunodeficiency virus (BIV) transactivator gene (tat) were cloned from virally infected cells and characterized. BIV expresses two distinct tat mRNAs composed of three exons that are derived by alternative splicing. The BIV tat mRNA splice variants encode Tat proteins of 103 (Tat103) and 108 (Tat108) amino acids. The Tat103 coding region is specified only by exon 2, while that of Tat108 is specified by a truncated exon 2 and the first 30 nt of exon 3. Thus, the first 98 amino acids of each Tat are identical, and have amino terminal, cysteine-rich, conserved core, basic, and carboxyl-terminal domains similar to Tats encoded by primate lentiviruses. BIV-infected bovine cells express a 14-kDa phosphorylated Tat protein identical in size to recombinant Tat expressed in bacteria. BIV Tat was shown to localize exclusively in the nucleoli of virally infected and Tat-expressing cells. Reporter gene assays indicated that Tat103 and Tat108 can strongly transactivate the BIV long terminal repeat (LTR) in virally permissive canine Cf2Th and nonpermissive HeLa and mouse NIH 3T3 cells, but not in permissive lapine EREp cells. However, an intact BIV tat gene is required for viral replication in both Cf2Th and EREp cells. Strong LTR activation by BIV Tat requires a TAR (transactivation responsive) element delimited by viral nt +1 to +31 and the Tat basic domain. BIV Tat strongly cross-transactivates the HIV-1 LTR in a TAR-dependent manner in Cf2Th, but not in EREp, HeLa, or NIH 3T3 cells. In contrast, strong, TAR-dependent cross-transactivation of the BIV LTR by HIV-1 Tat could not be demonstrated in any of these cell types. In Cf2Th cells Tat108 effects a moderately stronger transactivation of the BIV LTR than Tat103, indicative of a functional difference in BIV Tat proteins encoded by the mRNA splice variants. The present studies demonstrate that BIV Tat parallels the primate lentiviral Tats in structure and biochemistry but is not interchangeable with the latter.
authors.affiliation: Laboratory of Cell and Molecular Structure, SAIC Frederick, NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA. Fong@mail.ncifcrf.gov
authors.forename: S E
authors.initials: SE
authors.lastname: Fong
authors.forename: J D
authors.initials: JD
authors.lastname: Greenwood
authors.forename: J C
authors.initials: JC
authors.lastname: Williamson
authors.forename: D
authors.initials: D
authors.lastname: Derse
authors.forename: L A
authors.initials: LA
authors.lastname: Pallansch
authors.forename: T
authors.initials: T
authors.lastname: Copeland
authors.forename: L
authors.initials: L
authors.lastname: Rasmussen
authors.forename: A
authors.initials: A
authors.lastname: Mentzer
authors.forename: K
authors.initials: K
authors.lastname: Nagashima
authors.forename: G
authors.initials: G
authors.lastname: Tobin
authors.forename: M A
authors.initials: MA
authors.lastname: Gonda
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D002466:Cell Nucleolus; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004279:DNA, Viral; D004285:Dogs; D015696:Gene Products, tat / Q000032:analysis / Q000235:genetics*; D016325:HIV Long Terminal Repeat; D015497:HIV-1; D006367:HeLa Cells; D006801:Humans; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics* / Q000502:physiology; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010766:Phosphorylation; D012091:Repetitive Sequences, Nucleic Acid; D015534:Trans-Activators; D015533:Transcriptional Activation; D014779:Virus Replication; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D015696:Gene Products, tat; D015534:Trans-Activators; D054322:tat Gene Products, Human Immunodeficiency Virus