Cloning and sequencing of a full-length sea bream (Sparus aurata) beta-actin cDNA.
A full-length cDNA clone encoding beta-actin (beta-actin) was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. The sea bream beta-actin sequence showed 98% identity to carp and human beta-actin and 95% and 94% identity to sea squirt and Dictyostelium cytoplasmic actins, respectively.
Santos
C R
CR
CCMAR, Universidade do Aloarve, Portugal.
Power
D M
DM
Kille
P
P
Llewellyn
L
L
Ramsurn
V
V
Wigham
T
T
Sweeney
G E
GE
D000199:Actins / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D000199:Actins; D018076:DNA, Complementary