Cerebrospinal-fluid ciliary neurotrophic factor in neurological patients.
We developed a double sandwich immunoassay for the dosage of ciliary neurotrophic factor (CNTF) in cerebrospinal fluid (CSF). The detection limit was 100 pg/ml. This assay was applied to human CSF samples from 14 normal subjects, 26 patients with multiple sclerosis (MS), 17 with Guillain-Barré syndrome (GBS) or chronic inflammatory demyelinating polyneuropathy (CIDP), and 22 with tumours of the central nervous system (CNS) or leucaemic meningosis (LM). Samples from normal control subjects and from patients with tumours did not contain detectable CNTF. Only 2 patients with LM were positive, and all the patients with inflammatory diseases of the CNS and peripheral nervous system were positive. The MS group presented a mean value of 240 pg/ml CNTF and the GBS/CIDP group a value of 430 pg/ml.
Massaro
A R
AR
Institute of Neurology, UCSC Medical School, Rome, Italy.
Soranzo
C
C
Carnevale
A
A
D001254:Astrocytoma / Q000134:cerebrospinal fluid / Q000175:diagnosis; D001929:Brain Edema / Q000134:cerebrospinal fluid / Q000175:diagnosis; D001932:Brain Neoplasms / Q000134:cerebrospinal fluid / Q000175:diagnosis; D002908:Chronic Disease; D020934:Ciliary Neurotrophic Factor; D003711:Demyelinating Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis; D005909:Glioblastoma / Q000134:cerebrospinal fluid / Q000175:diagnosis; D006801:Humans; D007118:Immunoassay; D017254:Leukemic Infiltration / Q000134:cerebrospinal fluid / Q000175:diagnosis; D008577:Meningeal Neoplasms / Q000134:cerebrospinal fluid / Q000175:diagnosis; D008578:Meninges / Q000473:pathology; D008579:Meningioma / Q000134:cerebrospinal fluid / Q000175:diagnosis; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000175:diagnosis; D009419:Nerve Tissue Proteins / Q000134:cerebrospinal fluid*; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D011115:Polyneuropathies / Q000134:cerebrospinal fluid / Q000175:diagnosis; D011129:Polyradiculoneuropathy / Q000134:cerebrospinal fluid / Q000175:diagnosis; D012016:Reference Values
D020934:Ciliary Neurotrophic Factor; D009419:Nerve Tissue Proteins