title: Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine.
abstract: Characterization of the dnmZ, dnmU, and dnmV genes from the daunorubicin-producer Streptomyces peucetius by DNA sequence analysis indicated that these genes encode a protein of unknown function plus a putative thymidine diphospho-4-keto-6-deoxyglucose-3(5)-epimerase and thymidine diphospho-4-ketodeoxyhexulose reductase, respectively. Inactivation of each of the three genes by gene disruption and replacement in the wild-type strain demonstrated that all of them are required for daunosamine biosynthesis.
authors.affiliation: School of Pharmacy, University of Wisconsin, Madison 53706, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Otten
authors.forename: M A
authors.initials: MA
authors.lastname: Gallo
authors.forename: K
authors.initials: K
authors.lastname: Madduri
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: C R
authors.initials: CR
authors.lastname: Hutchinson
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002237:Carbohydrate Dehydrogenases / Q000235:genetics*; D002238:Carbohydrate Epimerases / Q000235:genetics*; D003001:Cloning, Molecular; D003630:Daunorubicin / Q000096:biosynthesis*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D002237:Carbohydrate Dehydrogenases; C107149:dnmV protein, Streptomyces peucetius; D002238:Carbohydrate Epimerases; C085762:thymidine-diphospho-4-keto-6-deoxyglucose epimerase; D003630:Daunorubicin