Homologues of the 24-kDa flagellar Ca(2+)-binding protein gene of Trypanosoma cruzi are present in other members of the Trypanosomatidae family.
A full-length cDNA encoding the 24-kDa flagellar Ca(2+)-binding protein (FCaBP) of the Dm28c clone of Trypanosoma cruzi was cloned and characterized. Comparison of the deduced amino acid sequence with those of the FCaBPs of other T. cruzi strains revealed greater than 97% sequence conservation. FCaBP-like genes are found in Trypanosoma conorhini, Trypanosoma freitasi, Trypanosoma lewisi, Herpetomonas megaseliae, Leptomonas seymouri, and Phytomonas serpens, but not in Crithidia deanei, Leishmania amazonensis, or Endotrypanum schaudinni: Among various T. cruzi strains, FCaBP genes are located on chromosomes of different size, although all strains possess multiple FCaBP genes organized as tandemly arranged gene families. Northern and Western blot analyses revealed that FCaBP mRNAs are produced in all organisms possessing FCaBP-hybridizing sequences, indicating that expression of FCaBP or an FCaBP-like protein is common to a number of trypanosomatid species.
Maldonado
R A
RA
Department of Biochemistry and Molecular Biology, Fundaçao Oswaldo Cruz, Rio de Janeiro, Brazil.
Linss
J
J
Thomaz
N
N
Olson
C L
CL
Engman
D M
DM
Goldenberg
S
S
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002135:Calcium-Binding Proteins / Q000235:genetics*; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005407:Flagella / Q000737:chemistry; D008969:Molecular Sequence Data; D016053:RNA, Protozoan / Q000032:analysis / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D014351:Trypanosomatina / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure
D002135:Calcium-Binding Proteins; D016054:DNA, Protozoan; D016053:RNA, Protozoan