Expression of 65-kDa oncofetal protein in experimental hepatoma after anticancer therapy.
We have tested the expression of a 65-kDa oncofetal protein (p65) after combined treatment with menadione and methotrexate in hamsters transplanted with Kirkman-Robins hepatoma. The treatment of tumor-bearing animals with these compounds significantly inhibited both the tumor development and the expression of p65. This inhibition in tumor tissue was calculated from densitograms of Western blots. The inhibition of p65 expression was also confirmed in the serum of hepatoma bearing animals by using solid-phase radioimmunoassay (RIA) to quantify the specificity of polyclonal antibodies to fetal p65 molecules. Additionally, p65 was shown to localize both in cytoplasm and in the nuclear extracts prepared from hepatoma tissue.
Mirowski
M
M
Department of Biochemistry, Medical University, Lodz, Poland.
Rozalski
M
M
Krajewska
U
U
Hanausek
M
M
Wierzbicki
R
R
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000187:drug effects / Q000276:immunology; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage*; D006224:Cricetinae; D003598:Cytoskeletal Proteins; D004347:Drug Interactions; D004591:Electrophoresis, Polyacrylamide Gel; D006490:Hemostatics / Q000008:administration & dosage; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008727:Methotrexate / Q000008:administration & dosage; D008840:Microfilament Proteins; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000276:immunology; D011863:Radioimmunoassay; D014812:Vitamin K / Q000008:administration & dosage
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000964:Antimetabolites, Antineoplastic; D003598:Cytoskeletal Proteins; D006490:Hemostatics; D008840:Microfilament Proteins; D010750:Phosphoproteins; C031192:oncofetal antigens; D014812:Vitamin K; C066559:Lcp1 protein, mouse; D008727:Methotrexate