Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae.
Thymidylate synthase converts deoxyuridylate to deoxythymidylate. The thyA gene has been cloned and sequenced from Neisseria gonorrhoeae MS11. This gene has an open reading frame of 795-801 bp and encodes a product which shares 71% identity with its Escherichia coli homolog. Unlike its E. coli counterpart, gonococcal thyA has a large, upstream transcribed region (300+ bp) that lacks a translatable reading frame. Gonococcal thyA is capable of complementing an E. coli thyA null mutant and shares similar levels of sensitivity with trimethoprim.
Carlson
J H
JH
Department of Health and Human Services, National Institutes of Health, National Institute of Allergy and Infectious Diseases, USA. jcarlson@pop.niaid.nih.gov
Hill
S A
SA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D013940:Thymidylate Synthase / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D014295:Trimethoprim / Q000494:pharmacology
D012333:RNA, Messenger; D014295:Trimethoprim; D013940:Thymidylate Synthase