African swine fever virus-specific cytotoxic T lymphocytes recognize the 32 kDa immediate early protein (vp32).
African swine fever (ASF) virus-specific cytotoxic T lymphocyte (CTL) activity has been studied in a model in which SLA inbred minipigs were experimentally infected with an attenuated isolate of the virus. The CTL assays were performed using alveolar macrophages as target cells. The specific lysis is mediated by purified CD8+ lymphocytes but not by CD4+ cells and can be blocked by incubation with anti-SLA class I monoclonal antibodies. The purified CD8+ population produced high levels of interferon-gamma after ASF virus stimulation. In an attempt to define the viral proteins recognized by CTL, target cells infected with a recombinant vaccinia virus (VV) expressing the ASF virus p32, an immediate early protein during ASF virus replication, were recognized and lysed by CTL. This assay may be useful for VV recombinant screening in order to identify other potential target ASF virus proteins.
Alonso
F
F
Centro de Investigación en Sanidad Animal, INIA, Madrid, Spain.
Domínguez
J
J
Viñuela
E
E
Revilla
Y
Y
D000358:African Swine Fever Virus / Q000276:immunology*; D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D017874:Immediate-Early Proteins / Q000276:immunology*; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
D017874:Immediate-Early Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins