title: Lack of interleukin 10 expression in monocyte-derived macrophages in response to in vitro infection by HIV type 1 isolates.
abstract: Interleukin 10 is a pleiotropic cytokine that is overexpressed in HIV-infected patients. Here, we investigated IL-10 expression in primary cultures of monocyte-derived macrophages (MDMs) in response to in vitro infection by HIV-1/Ba-L or two macrophage-tropic HIV-1 primary isolates. Whatever the multiplicity of infection used, and in spite of high replication levels and an increase in HIV-infected cell frequency, neither significant IL-10 secretion nor IL-10 mRNA overexpression was induced in HIV-1-infected MDMs. Moreover, identical results were obtained with HIV-1-infected 1-day monocytes. These results show that MDM infection by HIV is not sufficient by itself for inducing IL-10 synthesis.
authors.affiliation: Département de Recherche Médicale, Institut Paris-Sud sur les Cytokines, Centre de Recherches du Service de Santé des Armées, Fontenay aux Roses, France.
authors.forename: N
authors.initials: N
authors.lastname: Dereuddre-Bosquet
authors.forename: P
authors.initials: P
authors.lastname: Clayette
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: O
authors.initials: O
authors.lastname: Benveniste
authors.forename: P
authors.initials: P
authors.lastname: Fretier
authors.forename: P
authors.initials: P
authors.lastname: Jaccard
authors.forename: B
authors.initials: B
authors.lastname: Vaslin
authors.forename: A
authors.initials: A
authors.lastname: Lebeaut
authors.forename: D
authors.initials: D
authors.lastname: Dormont
mesh_terms: D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000276:immunology; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D019562:Viral Load; D014779:Virus Replication
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D054303:HIV Reverse Transcriptase