title: Nonexponential kinetics of a single tRNAPhe molecule under physiological conditions.
abstract: The fluorescence decay functions of individual, specifically labeled tRNAPhe molecules exhibit nonexponential character as a result of conformational dynamics occurring during the measurement on a single molecule. tRNAPhe conformational states that interchange much more slowly are evidenced by the distribution of lifetimes observed for many individual molecules. A structural model for the nonexponential decay indicates that the tRNAPhe-probe adduct fluctuates between two states, one of which provides conditions that quench the probe fluorescence.
authors.affiliation: Chemistry Department, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Jia
authors.forename: A
authors.initials: A
authors.lastname: Sytnik
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Vladimirov
authors.forename: B S
authors.initials: BS
authors.lastname: Cooperman
authors.forename: R M
authors.initials: RM
authors.lastname: Hochstrasser
mesh_terms: D003198:Computer Simulation; D005453:Fluorescence; D007700:Kinetics; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D012360:RNA, Transfer, Phe / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012360:RNA, Transfer, Phe