[Alteration of mRNA transcripts of rat Sertoli cell secreted proteins and Leydig cell LH/CG-R protein following glycosides of Tripterigium Wilfordii Hook administration].
Our studies were undertaken to assess the male antifertility mechanism of the total glycosides extracted from Tripterigium Wilfordii Hook. f. (GTW). In experimental group I, GTW was given by gastric gavage at a dose of 30 mg/kg per day for 35 days. In experimental group I, GTW was given in the same way at a dose of 10 mg/kg per day for 49 days. The transcripts level analysis indicated that in all the experimental groups ABP, SGP-2 and LH/CG-R mRNA were increased. Two ABP and two LH/CG-R mRNA transcripts with respective molecular sizes of 1.7 kb, 2.3 kb and 1.2 kb, 4.4 kb were identified. ABP and LH/ CG-R mRNA with 1.7 kb and 1.2 kb increased, while those with 2.3 and 4.4 kb remained unchanged. In addition, the changes of different proteins at the mRNA level were shown to be GTW dose-dependent. The morphological features of germ cell degeneration and appearance of germ cells in tubular lumen suggest that the increases in transcripts level of three proteins in testis may not be directly associated with the effect of GTW. The question whether GTW interfere with the function of sertoli cells and leydig cells would be study.
Zheng
W
W
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
Ye
S
S
He
X
X
Chen
Y
Y
Huang
Y
Y
D000727:Androgen-Binding Protein / Q000235:genetics*; D000818:Animals; D051152:Clusterin; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D006023:Glycoproteins / Q000235:genetics*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D007985:Leydig Cells / Q000378:metabolism; D008297:Male; D018832:Molecular Chaperones; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011974:Receptors, LH / Q000096:biosynthesis / Q000235:genetics*; D012708:Sertoli Cells / Q000378:metabolism; D014158:Transcription, Genetic; D029757:Tripterygium
D000727:Androgen-Binding Protein; D051152:Clusterin; D004365:Drugs, Chinese Herbal; D006023:Glycoproteins; D006027:Glycosides; D018832:Molecular Chaperones; D012333:RNA, Messenger; D011974:Receptors, LH