[Highly metastatic model of human hepatocellular carcinoma established in nude mice using orthotopic organ selection of metastatic variant from patient specimens].
We succeeded in establishing a highly metastatic model of human hepatocellular carcinoma (HCC) in athymic nude mice (LCI-D20). The metastatic variant was obtained by using orthotopic implantation of histologically intact tumor tissue selected from 30 fresh surgical specimens. It exhibited various features seen in clinical liver cancer patients: local growth, regional invasion, spontaneous intrahepatic, lymphnode and pulmonary metastases, and peritoneal seeding with bloody ascites. All mice with the implant-tumor died within 6 weeks due to serious metastasis. The 100% rate of transplantability and metastasis maintained for over 16 passages. The morphological characteristics of implant tumor cells were similar to those of the original specimen by histological and electronmicroscopic observation, and kept on secreting alpha-fetoprotein (AFP) in recipient animals. Those data from DNA content analysis by flow cytometry (D. I. value, 1.61) and chromosome karyotype revealed the existence of hypotriploid and hypertriploid cells. The results demonstrated that orthotopic implantation model of human HCC displayed features of human clinical HCC in animals. It should allow development of new treatment modalities and study of metastatic mechanism of human HCC.
Sun
F
F
Liver Cancer Institute, Shanghai Medical University.
Tang
Z
Z
Liu
K
K
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008175:Lung Neoplasms / Q000556:secondary*; D008207:Lymphatic Metastasis; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation / Q000379:methods