title: Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome.
abstract: A 36 kb sequence of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome at around 55 degrees has been determined. The sequenced region contains 36 ORFs including the phoB and groESL genes, and the whole rrnE operon. The phoB gene is transcribed in the direction opposite to that of chromosome replication, while most ORFs, including groESL and the rrnE operon, are transcribed in the same direction. Two newly identified tRNA genes upstream of the rrnE operon were those for Arg-tRNA and Gly-tRNA. The sequenced region contains an operon consisting of genes for degradation and uptake of mannan. The rrnE operon and its downstream ORFs are well conserved among Mycoplasma genitalium, Haemophilus influenzae, Synechocystis sp. and Methanococcus jannaschii. delta H consensus sequences are present in the promoter regions of three ORFs, including groESL.
authors.affiliation: Radioisotope Center, National Institute of Genetics, Mishima 411, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Sadaie
authors.affiliation: Radioisotope Center, National Institute of Genetics, Mishima 411, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yata
authors.affiliation: Radioisotope Center, National Institute of Genetics, Mishima 411, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Fujita
authors.affiliation: Laboratory for Pharmacology, Asahi Chemical Industry Co. Ltd, Shizuoka, Ohito 410-23, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sagai
authors.affiliation: Mitsubishi-kasei Institute of Life Sciences, Minami-Oya 11, Machida-shi, Tokyo 194, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Itaya
authors.affiliation: Advanced Institute of Science and Technology, Nara, Ikoma 630-01, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kasahara
authors.affiliation: Advanced Institute of Science and Technology, Nara, Ikoma 630-01, Japan.
authors.forename: Naotake
authors.initials: N
authors.lastname: Ogasawara
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018833:Chaperonins / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics; D000458:Cyanobacteria / Q000235:genetics; D004247:DNA / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D006193:Haemophilus influenzae / Q000235:genetics; D006361:Heating / Q000379:methods; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012407:rRNA Operon / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C097970:GroESL protein, Bacteria; C061278:PhoB protein, Bacteria; D004247:DNA; D012343:RNA, Transfer; D018833:Chaperonins