title: Results of laser tissue soldering in vasovasostomy and epididymovasostomy: experience in the rat animal model.
abstract: The use of microsurgical techniques (vasovasostomy and epididymovasostomy) for vasectomy reversal has now enabled surgeons to perform both procedures with certainly acceptable success rates. However, these operations are technically demanding and require special training in microsurgery.
  
  
  OBJECTIVE
  A new method of performing these procedures using laser tissue soldering is described and results are evaluated. Laser tissue soldering is different from laser welding in that it involves the laser activation of a protein solder with a dye specific for the specific wavelength of laser light; therefore, surrounding tissue is not affected by the laser.
  
  
  METHODS
  Ten rats underwent bilateral vasovasostomy and eleven underwent bilateral epididymovasostomy. In each rat, a sutured anastomosis was performed on one side while laser tissue soldering was performed on the other. Animals were sacrificed after one month and anastomoses were evaluated for patency and presence of sperm granulomas. Histologic analysis was also performed.
  
  
  RESULTS
  Patency rates were 8/10 (80%) for sutured vasovasostomy versus 9/10 (90%) for the laser technique. Epididymovasostomy patency rates were 8/11 (73%) for sutured versus 9/11 (82%) for the laser technique. Mean operative times were significantly shorter for lasered anastomoses when compared to controls. The frequency of granuloma formation did not significantly differ between laser and control groups.
  
  
  CONCLUSIONS
  Laser tissue soldering resulted in similar patency when compared to a conventional 2 layered sutured anastomosis while decreasing operative time. In addition, since fewer sutures are placed, the laser method is less technically demanding.
authors.affiliation: Scott Department of Urology, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: E K
authors.initials: EK
authors.lastname: Seaman
authors.forename: E D
authors.initials: ED
authors.lastname: Kim
authors.forename: A J
authors.initials: AJ
authors.lastname: Kirsch
authors.forename: Y C
authors.initials: YC
authors.lastname: Pan
authors.forename: S
authors.initials: S
authors.lastname: Lewitton
authors.forename: L I
authors.initials: LI
authors.lastname: Lipshultz
mesh_terms: D000818:Animals; D004822:Epididymis / Q000601:surgery; D005069:Evaluation Studies as Topic; D053685:Laser Therapy; D008297:Male; D008866:Microsurgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D014669:Vasovasostomy / Q000379:methods*