title: Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, Telapia (Telapia mossambica).
abstract: Strains of Aeromonas hydrophila isolates from skin lesions of the common freshwater fish, Telapia mossambica, were screened for the presence of plasmid DNA by agarose gel electrophoresis and tested for susceptibility to 10 antimicrobial agents. Of the 21 fish isolates examined, all were resistant to ampicillin and sensitive to gentamycin. Most isolates were resistant to streptomycin (57%), tetracycline (48%) and erythromycin (43%). While seven of 21 isolates harboured plasmids, with sizes ranging from 3 to 63.4 kilobase pair (kb), it was only possible to associate the presence of a plasmid with antibiotic resistance (ampicillin and tetracycline) in strain AH11. Both the plasmid and the associated antimicrobial resistance could be transferred to an Escherichia coli recipient by single-step conjugation at a frequency of 4.3 x 10(-3) transconjugants per donor cell.
authors.affiliation: Department of Biotechnology, Faculty of Food Science and Biotechnology, University Pertanian Malaysia, Selangor, Malaysia.
authors.forename: R
authors.initials: R
authors.lastname: Son
authors.forename: G
authors.initials: G
authors.lastname: Rusul
authors.forename: A M
authors.initials: AM
authors.lastname: Sahilah
authors.forename: A
authors.initials: A
authors.lastname: Zainuri
authors.forename: A R
authors.initials: AR
authors.lastname: Raha
authors.forename: I
authors.initials: I
authors.lastname: Salmah
mesh_terms: D016980:Aeromonas hydrophila / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D000668:Ampicillin Resistance / Q000235:genetics; D000818:Animals; D017756:Aquaculture; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology; D011815:R Factors / Q000235:genetics / Q000302:isolation & purification; D013307:Streptomycin / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics; D017210:Tilapia / Q000382:microbiology*
chemical_list: D005839:Gentamicins; D004917:Erythromycin; D013307:Streptomycin