Interaction of the UV-damaged DNA-binding protein with hepatitis B virus X protein is conserved among mammalian hepadnaviruses and restricted to transactivation-proficient X-insertion mutants.
We carried out a comparative analysis of several proposed host protein partners of the human hepatitis B virus X protein (HBx) using both the GAL4- and the LexA-based yeast two-hybrid system. We showed that the interaction of HBx with the UV-damaged DNA-binding protein (UVDDB) is positive in both yeast systems, detectable in cotransfected human cells, conserved by rodent hepadnavirus X proteins (known to transactivate in human cells), and tightly correlated with the transactivation proficiency of X-insertion mutants. Taken together, our results strongly suggest that UVDDB is involved in X-mediated transactivation.
Sitterlin
D
D
Unité de Recombinaison et Expression Génétique (INSERM U163), Institut Pasteur, Paris, France.
Lee
T H
TH
Prigent
S
S
Tiollais
P
P
Butel
J S
JS
Transy
C
C
D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000528:radiation effects; D015613:Hepadnaviridae / Q000235:genetics*; D006801:Humans; D009154:Mutation; D015534:Trans-Activators / Q000502:physiology*; D015533:Transcriptional Activation; D014466:Ultraviolet Rays; D054334:Viral Regulatory and Accessory Proteins
D004268:DNA-Binding Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein