title: Microdialysis monitoring of extracellular glutamate combined with the simultaneous recording of evoked field potentials in hippocampal organotypic slice cultures.
abstract: These experiments combined extracellular electrophysiological multirecordings from hippocampal organotypic slice cultures with application of drugs to and sampling of extracellular fluid from a restricted region of the slice using a microdialysis probe. Glutamate (Glu) concentrations were monitored in 0.5 or 2 min microdialysis samples, while evoked field potentials responses (EvFPR) in the CA1 region of the hippocampus (stimulation in the CA3 area) were simultaneously recorded using a multi-electrodes array (Physiocard). Glu was assayed by capillary electrophoresis with laser-induced fluorescence detection combined with a continuous flow derivatization of dialysates. The performance of this combined approach was demonstrated by monitoring extracellular Glu concentrations and EvFPR after K+ induced depolarisation, Glu uptake blockade by trans-pyrrolidine-2,4-dicarboxylic acid (PDC), and electrical stimulation. Such an approach allows a global monitoring of the neuronal functioning with a fine time resolution (up to 30 s) on a simple in vitro brain slice model, to be used as a complement to conventional in vivo microdialysis studies.
authors.affiliation: Département de Pharmacologie, Centre Médical Universitaire, Geneva, Switzerland. frobert@cmu.unige.ch
authors.forename: F
authors.initials: F
authors.lastname: Robert
authors.forename: L
authors.initials: L
authors.lastname: Parisi
authors.forename: L
authors.initials: L
authors.lastname: Bert
authors.forename: B
authors.initials: B
authors.lastname: Renaud
authors.forename: L
authors.initials: L
authors.lastname: Stoppini
mesh_terms: D000818:Animals; D003998:Dicarboxylic Acids / Q000494:pharmacology; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005071:Evoked Potentials / Q000187:drug effects; D018698:Glutamic Acid / Q000032:analysis / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods; D008839:Microelectrodes; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D009924:Organ Culture Techniques; D011188:Potassium / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D003998:Dicarboxylic Acids; D014179:Neurotransmitter Uptake Inhibitors; D011759:Pyrrolidines; D018698:Glutamic Acid; C083486:pyrrolidine-2,4-dicarboxylic acid; D011188:Potassium