Polymorphic microsatellite DNA markers in the rabbit (Oryctolagus cuniculus).
By searching the EMBL nucleotide database a total of 157 rabbit nuclear gene microsatellites were obtained (VAN LITH and VAN ZUTPHEN, Animal Genetics 27, 387-395, 1996). Thirteen of these were analysed by PCR to examine the degree of polymorphism of the amplified fragments in rabbits from different breeds. The 13 pairs of primers resulted in polymorphic products with an average of four alleles per microsatellite sequence (ranging between 2-11). There was a positive relationship between the longest repeat unit number in the nucleotide sequence and the number of alleles detected. The results obtained so far justify the conclusion that rabbit microsatellites extracted from the EMBL nucleotide sequence database are sufficiently polymorphic to be useful as Type 1 markers in rabbit genetic studies.
van Haeringen
W A
WA
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Graduate School of Animal Health, Utrecht University, The Netherlands.
den Bieman
M
M
van Zutphen
L F
LF
van Lith
H A
HA
D000483:Alleles; D000818:Animals; D001345:Autoradiography / Q000662:veterinary; D001483:Base Sequence; D001947:Breeding; D002874:Chromosome Mapping; D004276:DNA, Satellite / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D005819:Genetic Markers; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D011817:Rabbits / Q000235:genetics*
D004276:DNA, Satellite; D005819:Genetic Markers