A random DNA sequencing, computer-based approach for the generation of a gene map of molluscum contagiosum virus.
The genome of Molluscum contagiosum virus (MCV) has a high G + C content, which largely differs from those of vaccinia virus (VAC) and other characterized poxviruses. This has precluded the use of DNA hybridization for the identification of MCV genes and the further establishment of the virus genetic map. To circumvent this problem, we have partially sequenced clones containing virus restriction endonuclease fragments, which were derived by either single or double-digestion of genomic DNA from the subtype I of MCV. The DNA sequences were translated and used to search protein data bases. This analysis resulted in the finding of high-scoring matches to data base entries, including forty-five VAC genes. In addition, MCV-specific sequences that encoded protein domains of known function (i.e. DNA J domain) were found. The locations of MCV clones were inferred from the presumed colinearity of both MCV and VAC genomes, and further confirmed by PCR technology. The data presented here led to the construction of a partial genetic map of MCVI, which revealed that the order and orientation of a large number of MCV genes were equivalent to those of their VAC homologues. The conserved gene arrangement was apparently disrupted in the terminal regions, where MCV sequences showing homologies with the VAC counterparts were not found.
Moratilla
M
M
Centro Nacional de Biotecnología, C.S.I.C., Cantoblanco, Spain.
Agromayor
M
M
Nuñez
A
A
Funes
J M
JM
Varas
A J
AJ
Lopez-Estebaranz
J L
JL
Esteban
M
M
Martin-Gallardo
A
A
D001482:Base Composition; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D016208:Databases, Factual; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D008977:Molluscum contagiosum virus / Q000235:genetics*; D016366:Open Reading Frames; D014616:Vaccinia virus / Q000235:genetics
D004279:DNA, Viral