Fucosylated glycoproteins in Chinese hamster metaphase chromosomes.
Distribution of fucosylated proteins in Chinese hamster metaphase chromosomes was studied with a fluorescein isothiocyanate conjugated monofucosyl-specific lectin, Ulex europaeus agglutinin I (UEA I). In situ binding of UEA I showed that fucosylated chromosomal proteins are preferentially localized to two internal structural domains of metaphase chromosomes: the helically coiled substructure of chromatids, and the Q-band regions. Corresponding proteins were identified in Western blots of isolated metaphase chromosomes. Several proteins with molecular weights ranging from 33 to 195 kD were recognized by UEA I. These data suggest that a subset of chromosomal proteins are fucosylated; they may have a role in the structural organization of chromosomes.
Myllyharju
J
J
Department of Biology, University of Turku, Finland.
Nokkala
S
S
D000818:Animals; D002875:Chromosomes / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D006023:Glycoproteins / Q000737:chemistry*; D008677:Metaphase; D008970:Molecular Weight
D006023:Glycoproteins; C016465:fucoproteins