title: Isolation and characterization of a cDNA clone encoding an osmotin-like protein from Arabidopsis thaliana.
abstract: A phage library of cDNA from Arabidopsis thaliana has been screened with oligodeoxyribonucleotides designed from regions of high homology found in tobacco osmotin and other osmotin-like proteins. One of the selected clones, Atosm34, presents a 734 bp open reading frame encoding a polypeptide of 244 amino acids, including the putative N-terminal signal and C-terminal propeptide sequences. Comparative alignment reveals extensive homologies to osmotin and the osmotin-like proteins found in Solanaceae, and also to a related polypeptide found in soybean. Genomic hybridization suggests that the cDNA obtained here corresponds to a single copy gene, and RNA blot analysis showed that the level of expression is highest in old leaves.
authors.affiliation: Laboratoire de Biochimie et Physiologie Végétales, Université de Geneve, Switzerland.
authors.forename: N
authors.initials: N
authors.lastname: Capelli
authors.forename: T
authors.initials: T
authors.lastname: Diogon
authors.forename: H
authors.initials: H
authors.lastname: Greppin
authors.forename: P
authors.initials: P
authors.lastname: Simon
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000145:classification / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant; D015870:Gene Expression; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D018749:RNA, Plant; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; C107039:Atosm34 protein, Arabidopsis; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant