Culture of Helicobacter pylori from gastric biopsies transported in biopsy urease test tubes.
Gastric biopsy specimens of 57 consecutively observed dyspeptic patients were studied for the presence of Helicobacter pylori by histological examination, biopsy urease test (BUT) and culture. For culture, biopsy samples were transported in both Stuart media and BUT tubes. All 15 isolates could be cultured from both Stuart and BUT tubes. Thus, if the main reason for culture of Helicobacter pylori is for antimicrobial susceptibility testing, only positive BUT tubes need to be submitted. This would reduce both the expense and the number of biopsies needed.
Rautelin
H
H
Department of Bacteriology and Immunology, Haartman Institute, Helsinki, Finland.
Seppälä
K
K
Nuutinen
H
H
Kärkkäinen
P
P
Sipponen
P
P
Kosunen
T U
TU
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D001706:Biopsy; D003470:Culture Media; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000201:enzymology / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D014510:Urease / Q000378:metabolism*
D003470:Culture Media; D014510:Urease