Afferent innervation of gastrointestinal tract smooth muscle by the hepatic branch of the vagus.
To survey the vagal hepatic branch afferent projections to and the terminal specializations in the gastrointestinal tract, male Sprague-Dawley rats were given subdiaphragmatic vagotomies, sparing only the common hepatic branch, and were injected with 3 microl of 8% wheat germ agglutinin-horseradish peroxidase in the left nodose ganglion. The nodose ganglia, the stomach, the first 8 cm of duodenum, and the cecum were prepared as wholemounts and were processed with tetramethyl benzidine. Hepatic afferent innervation of the ventral stomach consisted of one or more bundles entering at the lower esophageal sphincter and coursing to the forestomach, where they branched into distinct terminal fields. The only fibers on the dorsal forestomach were distal branches and terminals that wrapped around the greater curvature from the ventral side. Hepatic afferents supplied the forestomach with both intraganglionic laminar endings (IGLEs; putative mechanosensors that coordinate peristalsis) and intramuscular arrays (IMAs; considered tension receptors). IGLEs were located primarily on the ventral wall of the stomach, whereas IMAs were distributed symmetrically. Afferents were also supplied to the distal antrum and the pylorus, with pyloric innervation consisting almost exclusively of IMAs. Innervation of the proximal duodenum was denser in the first 3 cm and decreased progressively caudally, with only meager innervation after 6 cm. Cecal innervation consisted of a few fibers at the ileocecal junction. Duodenal and cecal endings were predominately IGLEs. These results indicate that the hepatic branch carries sensory information from the forestomach, antrum, pylorus, duodenum, and cecum. Furthermore, the different terminals it supplies suggest that the branch mediates a multiplicity of gastrointestinal functions.
Phillips
R J
RJ
Purdue University, West Lafayette, Indiana 47907, USA.
Baronowsky
E A
EA
Powley
T L
TL
D000344:Afferent Pathways / Q000166:cytology / Q000502:physiology; D000465:Algorithms; D000818:Animals; D002452:Cell Count; D004064:Digestive System / Q000294:innervation*; D004386:Duodenum / Q000294:innervation; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D008099:Liver / Q000294:innervation*; D008297:Male; D009130:Muscle, Smooth / Q000294:innervation*; D009411:Nerve Endings / Q000502:physiology; D009412:Nerve Fibers / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013266:Stilbamidines; D014628:Vagotomy; D014630:Vagus Nerve / Q000502:physiology*; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate