Radiosensitivity of different aged human lymphocytes following electron irradiation in vitro.
Cytochalasin B-blocking micronucleus test and chromosomal aberration analysis were used in this study to estimate the yield of individual variability in radiation response of different aged human lymphocytes. Both analyses were performed in three groups of adults, aged 18-65 years, on two sampling times, following irradiation by therapeutical dose of 2 Gy e- in vitro. No statistically significant difference in the induced yield of exchange aberrations between individuals under consideration was found. The yield of total aberration data showed greater variability and was statistically significant in the oldest group against two other adult groups. Regarding to fixation times no statistically significant differences in the induced yield of chromosomal aberrations (exchanges as well as total aberrations) were observed. The study has shown a slight increase in spontaneously occurring micronuclei with age. Almost equal mean number of radiation induced micronuclei was observed in the groups of adults aged 18-20 and 45-55 years. The highest mean number was observed in the oldest group. Evident variation in number of radiation induced micronuclei among individuals from the same age group was observed. The results of micronuclei assay for all individuals under consideration show statistically significant difference in the yield of radiation-induced micronuclei regarding the second fixation time. This study has shown that cytochalasin-B blocking micronucleus test is more sensitive assay than chromosomal aberration analysis for the estimation of individual radiosensitivity.
Joksić
G
G
Vinca, Institute of Nuclear Sciences, Medical Protection Center, Beograd, Yugoslavia.
Nikolić
M
M
Spasojević-Tisma
V
V
D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000528:radiation effects; D002869:Chromosome Aberrations; D003571:Cytochalasin B / Q000494:pharmacology; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000528:radiation effects; D015162:Micronucleus Tests; D008875:Middle Aged; D011836:Radiation Tolerance
D003571:Cytochalasin B