title: The quantitative analysis of multiple mRNA species using oligonucleotide probes in an S1 nuclease protection assay.
abstract: The quantitative measurement of steady-state mRNA levels is fundamental to the analysis of gene expression. A variety of techniques are widely used to achieve this including Northern blotting, RNase protection, and S1 nuclease protection. We describe here in detail a relatively recent extension of the S1 nuclease protection technique (1) in which radiolabeled oligonucleotides are used as probes in a solution hybridization assay (2). The principle advantage of this technique is that it allows, in a single RNA sample, the simultaneous measurement of the relative levels of at least six mRNA species, including that of a control mRNA. Further, a large number of RNA samples can be analyzed at one time.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Alberta, Edmonton, Canada.
authors.forename: V A
authors.initials: VA
authors.lastname: Tanay
authors.forename: B P
authors.initials: BP
authors.lastname: Tancowny
authors.forename: T A
authors.initials: TA
authors.lastname: Glencorse
authors.forename: A N
authors.initials: AN
authors.lastname: Bateson
mesh_terms: D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism*
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D015719:Single-Strand Specific DNA and RNA Endonucleases