title: H-NS and RpoS regulate emergence of Lac Ara+ mutants of Escherichia coli MCS2.
abstract: Two master growth-phase regulatory proteins, H-NS and sigmaS, are involved in the formation of araB-lacZ fusion clones of Escherichia coli MCS2. The stationary-phase sigma factor RpoS is strictly required for the appearance of such mutants, whereas the histone-like protein H-NS represses their emergence. Our results support the idea that genetic changes leading to adaptive mutation in this model system are regulated by physiological signal transduction networks.
authors.affiliation: Servicio de Microbiologia, Hospital Ramón y Cajal, INSALUD, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Gómez-Gómez
authors.forename: J
authors.initials: J
authors.lastname: Blázquez
authors.forename: F
authors.initials: F
authors.lastname: Baquero
authors.forename: J L
authors.initials: JL
authors.lastname: Martínez
mesh_terms: D001089:Arabinose / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D001426:Bacterial Proteins / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005798:Genes, Bacterial; D007763:Lac Operon; D007785:Lactose / Q000378:metabolism; D009154:Mutation; D012808:Sigma Factor / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D001089:Arabinose; D007785:Lactose