In vitro production of tumor necrosis factor-alpha, interleukin-6 and interleukin-8 from normal human peripheral blood mononuclear cells stimulated by Rhodococcus equi.
The capability of heat-killed Rhodococcus equi organisms to induce in vitro release of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6 and IL-8 from normal human mononuclear cells as well as the secretion kinetics of these inflammatory cytokines over a 48 h period were evaluated. Results show that normal human mononuclear cells are efficiently triggered to secrete TNF-alpha, IL-6 and IL-8 following R. equi stimulation according to a different kinetics. In particular, release of IL-B was already maximally expressed after 2 h of stimulation, while TNF-alpha amounts progressively increased in a time-dependent fashion. Finally, IL-6 secretion reached peak levels as soon as 18 h of incubation. Taken together, these data point out that monocyte-derived cytokines may play an important role in the immunological control of R. equi infection in immunocompetent people.
Pece
S
S
Department of Internal Medicine, Immunology and Infectious Diseases, Bari University Medical School, Italy.
Giuliani
G
G
Fumarola
D
D
Mastroianni
C M
CM
Lichtner
M
M
Vullo
V
V
Antonaci
S
S
Jirillo
E
E
D002478:Cells, Cultured; D004731:Endotoxins / Q000032:analysis; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology* / Q000382:microbiology*; D016929:Rhodococcus equi / Q000276:immunology* / Q000472:pathogenicity; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D014774:Virulence
D004731:Endotoxins; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha