title: Cellular localization of human herpesvirus-6 in the brains of children with AIDS encephalopathy.
abstract: Human herpesvirus-6, the etiologic agent of exanthem subitum, is a ubiquitous virus that infects almost all children by the age of 2 years and that has previously been shown to be neuroinvasive. These characteristics suggest that human herpesvirus-6 may be important in the neuropathogenesis of acquired immune deficiency syndrome (AIDS) in children. To address this hypothesis, we evaluated postmortem pediatric brain tissues for the presence of human herpesvirus-6 infection. Using in situ hybridization with a digoxigenin-labeled DNA probe for the large tegument protein gene of human herpesvirus-6, we detected nuclear signals in postmortem brain tissue from 4/5 children with human immunodeficiency virus-1 encephalitis. Human herpesvirus-6 DNA was found in numerous oligodendrocytes of the white matter and less frequently in astrocytes, macrophages, microglia and neurons. The human herpesvirus-6 positive cells detected by in situ hybridization were not immunoreactive either for human herpesvirus-6 early nuclear phosphoproteins or for surface glycoproteins associated with productive infection. Only rare human herpesvirus-6 infected cells were found in age-matched control brain tissues. No human herpesvirus-6 infected cells were found in human fetal brain tissue. These data suggest that human herpesvirus-6 is more extensively disseminated in neural cells in the presence of human immunodeficiency infection and immunodeficiency in pediatric AIDS patients, and it may contribute to the pathogenesis of AIDS encephalopathy.
authors.affiliation: Department of Neurology, University of Rochester Medical Center, NY 14642, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: L R
authors.initials: LR
authors.lastname: Sharer
authors.forename: S
authors.initials: S
authors.lastname: Dewhurst
authors.forename: B M
authors.initials: BM
authors.lastname: Blumberg
authors.forename: C B
authors.initials: CB
authors.lastname: Hall
authors.forename: L G
authors.initials: LG
authors.lastname: Epstein
mesh_terms: D015526:AIDS Dementia Complex / Q000821:virology*; D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D001921:Brain / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007223:Infant; D008297:Male
chemical_list: D004279:DNA, Viral