[The isolation of the surface antigen from vegetative cells of Bacillus anthracis STI-1 and study of its protective properties].
The method for the extraction of native surface protein antigen with a mol. wt. of 92 kD from vegetative cells of B.anthracis STI-1 and its purification was developed. The antigen was extracted with 3% sodium lauryl sarcosylate at 4 degrees C from bacterial mass previously treated with 0.1 M tris-HCl buffer solution, pH 8.0 Purification was carried out by adsorption chromatography on hydroxylapatite. The isolated protein antigen with a mol. wt. of 92 kD was electrophoretically and immunochemically homogeneous. The study of the protective properties of this protein in combination with Freund's adjuvant, made on white mice, revealed that it had good prospects for use in the creation of chemical vaccines against the causative agent of anthrax.
Beznosov
M V
MV
Petrov
G A
GA
Sorkin
Iu I
IuI
Filippova
O L
OL
Rogov
S N
SN
D000818:Animals; D000881:Anthrax / Q000517:prevention & control; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D001408:Bacillus anthracis / Q000737:chemistry / Q000276:immunology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004353:Drug Evaluation, Preclinical; D007114:Immunization / Q000379:methods; D008722:Methods; D051379:Mice; D008970:Molecular Weight; D005719:gamma-Globulins / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D005719:gamma-Globulins