title: Transcription of the Leishmania major Hsp70-I gene locus does not proceed through the noncoding region.
abstract: Primary transcripts in kinetoplastid protozoa are generally assumed to be multicistronic. We have analyzed the transcription in the gene locus which encodes the 70-kDa heat shock protein by using nuclear run-on analysis. We find that RNA synthesis in the Hsp70-I gene locus either is terminated or pauses within the intergenic region approximately 250 nt downstream of the polyadenylation site. We therefore propose a discontinuous mode of transcription in the Hsp70 genes of Leishmania major.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Dresel
authors.forename: J
authors.initials: J
authors.lastname: Clos
mesh_terms: D000818:Animals; D017125:Genes, Protozoan; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics; D018320:Leishmania major / Q000235:genetics*; D005810:Multigene Family; D016366:Open Reading Frames; D015800:Protozoan Proteins / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism; D015347:RNA Probes / Q000737:chemistry; D016053:RNA, Protozoan / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C490310:Hsp83 protein, protozoan; D015800:Protozoan Proteins; D015347:RNA Probes; D016053:RNA, Protozoan; D012319:RNA Polymerase II; D015719:Single-Strand Specific DNA and RNA Endonucleases