title: Stage-specific requirement of cysteine during in vitro maturation of porcine oocytes for glutathione synthesis associated with male pronuclear formation.
abstract: The present study was designed to clarify the duration of maturation of porcine oocytes when cysteine promotes male pronuclear (MPN) formation through oocyte glutathione (GSH) synthesis. When cumulus-oocyte complexes (COCs) were cultured in a serum-free maturation medium supplemented or not supplemented with 0.57 mM cysteine, about 90% of oocytes reached metaphase I (M-I) to metaphase II (M-II) and M-II at 36 and 48 h of culture, respectively. When cysteine was added to medium at 0, 12, 24, and 36 h of culture and COCs were cultured for a total of 48 h, oocyte GSH concentrations at the end of culture and the incidence of MPN formation after sperm penetration in vitro were both higher than the values in oocytes cultured for 48 h without cysteine. In contrast, the GSH concentration at 48 h and the incidence of MPN formation were not increased when cysteine was present only during the first 24 h of maturation culture. When cysteine was added to medium every 3 h from 36 h of culture on, a higher incidence of MPN formation was obtained in oocytes cultured in the presence of cysteine from 36 to 42 h than from 36 to 45 h of culture, although GSH concentrations were higher in oocytes cultured with cysteine from 36 to 42 h than from 39 to 42 h of culture. These results suggest that the presence of cysteine in maturation medium is critical only between 42 and 48 h of culture when porcine oocytes are in the late M-I to M-II stage of development. At that time cysteine is utilized for GSH synthesis, which is subsequently instrumental in the formation of MPN after sperm penetration in vitro.
authors.affiliation: Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sawai
authors.forename: H
authors.initials: H
authors.lastname: Funahashi
authors.forename: K
authors.initials: K
authors.lastname: Niwa
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D016895:Culture Media, Serum-Free; D003545:Cysteine / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro; D005978:Glutathione / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D013084:Sperm-Ovum Interactions / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013552:Swine; D013997:Time Factors
chemical_list: D016895:Culture Media, Serum-Free; D005978:Glutathione; D003545:Cysteine