title: Expression of cholecystokinin messenger RNA in reciprocally-connected auditory thalamus and cortex in the rat.
abstract: Cholecystokinin exerts a potent antiepileptic action in mammalian auditory system and undergoes seizure-mediated up-regulation. The present study investigated cholecystokinin messenger RNA expression in the reciprocally-connected auditory thalamus and cortex in the rat. Immunofluorescence in situ hybridization was performed using a 24-base cholecystokinin-messenger RNA oligonucleotide probe. Corticothalamic projection neurons were identified by means of the retrograde fluorescent tracer rhodamine latex microspheres injected into the medial geniculate body. In our experiments, cholecystokinin messenger RNA transcripts were found in about 80% of neurons located within the reciprocally-connected regions of the medial geniculate body and the auditory cortices. These observations provide evidence of cholecystokinin production in the reciprocally-connected regions of the auditory thalamus and cortex, the structures which jointly create the thalamo-corticothalamic circuit which has been implicated in seizure genesis.
authors.affiliation: Loeb Research Institute, Ottawa Civic Hospital/University of Ottawa, Ontario, Canada.
authors.forename: V V
authors.initials: VV
authors.lastname: Senatorov
authors.forename: V L
authors.initials: VL
authors.lastname: Trudeau
authors.forename: B
authors.initials: B
authors.lastname: Hu
mesh_terms: D000818:Animals; D001306:Auditory Pathways / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D002766:Cholecystokinin / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013788:Thalamus / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D002766:Cholecystokinin