Gas chromatographic/mass spectrometric measurement of ascorbic acid and analysis of ascorbic acid degradation in solution.
L-Ascorbic acid, DHA, and the oxidized products derived from AA can be accurately measured using GC/MS. Owing to the complex nature of the reactions through which AA proceeds, we believe that GC/MS is currently the procedure of choice in making AA-related measurements. The methods described are useful in defining reactions involving AA. The methods may indicate in vivo oxidative injury and may allow the use of AA-derived products to determine if antioxidant modulations are effective.
Deutsch
J C
JC
Department of Medicine, University of Colorado Health Sciences Center, Denver 80220, USA.
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis* / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*
D001205:Ascorbic Acid