In situ detection of reovirus in formalin-fixed, paraffin-embedded chicken tissues using a digoxigenin-labeled cDNA probe.
An in situ hybridization (ISH) technique using a digoxigenin (DIG)-labeled cDNA probe detected avian reovirus (ARV) RNA in formalin-fixed, paraffin-embedded chicken tissues. Tissues were collected 3 and 10 days following inoculation with the R-2 or the S1133 strain of ARV. The cDNA clone HJp1, located on the S1 gene segment of the ARV S1133 strain, was used to prepare a nonradioactive probe. The ISH assay localized ARV RNA in infected tissues including heart, liver, intestine, pancreas, and tendon. No positive-stained cells occurred in sections from uninfected chickens.
Liu
H J
HJ
Department of Poultry Science, Auburn University, Alabama 36849-5416, USA.
Giambrone
J J
JJ
D000818:Animals; D002645:Chickens; D015342:DNA Probes; D018076:DNA, Complementary; D004076:Digoxigenin; D005557:Formaldehyde; D005814:Genes, Viral; D006652:Histological Techniques; D017403:In Situ Hybridization; D010232:Paraffin; D011201:Poultry Diseases; D012367:RNA, Viral / Q000032:analysis; D012087:Reoviridae / Q000302:isolation & purification*; D012088:Reoviridae Infections / Q000473:pathology / Q000662:veterinary*
D015342:DNA Probes; D018076:DNA, Complementary; D012367:RNA, Viral; D005557:Formaldehyde; D010232:Paraffin; D004076:Digoxigenin