title: The effect of viral regulatory protein expression on gene delivery by human immunodeficiency virus type 1 vectors produced in stable packaging cell lines.
abstract: We describe the generation of stable human immunodeficiency virus type 1 (HIV-1)-packaging lines that constitutively express high levels of HIV-1 structural proteins in either a Rev-dependent or a Rev-independent fashion. These cell lines were used to assess gene transfer by using an HIV-1 vector expressing the hygromycin B resistance gene and to study the effects of Rev, Tat, and Nef on the vector titer. The Rev-independent cell lines were created by using gag-pol and env expression vectors that contain the Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE). Vector titers approaching 10(4) CFU/ml were routinely obtained with these cell lines, as well as with the Rev-dependent cell lines, with HeLa-CD4 cells as targets. The presence of Nef and Tat in the producer cell each increased the vector titer 5- to 10-fold. Rev, on the other hand, was absolutely essential for gene transfer, unless the MPMV CTE was present in the vector. In that case, by using the Rev-independent cell lines for packaging, Rev could be completely eliminated from the system without a reduction in vector titer.
authors.affiliation: Myles H. Thaler Center for AIDS and Human Retrovirus Research and Department of Microbiology, University of Virginia, Charlottesville 22908, USA.
authors.forename: N
authors.initials: N
authors.lastname: Srinivasakumar
authors.forename: N
authors.initials: N
authors.lastname: Chazal
authors.forename: C
authors.initials: C
authors.lastname: Helga-Maria
authors.forename: S
authors.initials: S
authors.lastname: Prasad
authors.forename: M L
authors.initials: ML
authors.lastname: Hammarskjöld
authors.forename: D
authors.initials: D
authors.lastname: Rekosh
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D015693:Gene Products, nef / Q000502:physiology; D015694:Gene Products, rev / Q000502:physiology; D015696:Gene Products, tat / Q000502:physiology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D015678:Viral Structural Proteins / Q000235:genetics*; D019065:Virus Assembly; D014779:Virus Replication; D054311:nef Gene Products, Human Immunodeficiency Virus; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D015693:Gene Products, nef; D015694:Gene Products, rev; D015696:Gene Products, tat; D015678:Viral Structural Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus