Expanded bed adsorption for recovery of renatured human recombinant interleukin 8 from Escherichia coli inclusion bodies.
Expanded bed adsorption is a chromatography technique based on stable fluidization, designed for large scale recovery of proteins directly from particulate-containing feedstocks. Described in this article is the use of expanded bed adsorption for recovery of renatured recombinant human interleukin 8 from Escherichia coli inclusion bodies, using a cation exchange adsorbent. The yield of interleukin 8 from the expanded bed was approximately 100% and the purification factor was approximately 4. Following each interleukin 8 purification a cleaning in place procedure was performed with a commercial cleaner. The results show that the adsorbent can be used at least 50 times without any significant loss of function.
Barnfield Frej
A K
AK
Pharmacia Biotech AB, Uppsala, Sweden.
D000327:Adsorption; D002845:Chromatography / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry*; D005285:Fermentation; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry*; D016209:Interleukin-8 / Q000302:isolation & purification*; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000302:isolation & purification; D012995:Solubility
D016209:Interleukin-8; D011994:Recombinant Proteins