title: Type 1 ribosome-inactivating proteins are the most abundant proteins in iris (Iris hollandica var. Professor Blaauw) bulbs: characterization and molecular cloning.
abstract: The most abundant protein of Iris bulbs has been identified as a type 1 ribosome-inactivating protein (RIP). Analysis of the purified proteins and molecular cloning of the corresponding cDNAs demonstrated that this type 1 RIP is a mixture of three isoforms that exhibit a high degree of sequence identity and have similar, though not identical, ribosome-inactivating and polynucleotide:adenosine glycosidase activities. The accumulation of large quantities of type 1 RIP in a vegetative storage organ suggests that this presumed defence-related protein also plays a role in the nitrogen-storage metabolism of the bulb.
authors.affiliation: Laboratory for Phytopathology and Plant Protection, Catholic University of Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium.
authors.forename: E J
authors.initials: EJ
authors.lastname: Van Damme
authors.forename: A
authors.initials: A
authors.lastname: Barre
authors.forename: L
authors.initials: L
authors.lastname: Barbieri
authors.forename: P
authors.initials: P
authors.lastname: Valbonesi
authors.forename: P
authors.initials: P
authors.lastname: Rouge
authors.forename: F
authors.initials: F
authors.lastname: Van Leuven
authors.forename: F
authors.initials: F
authors.lastname: Stirpe
authors.forename: W J
authors.initials: WJ
authors.lastname: Peumans
mesh_terms: D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007498:Iris; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins