3-Ketoglycoside-mediated metabolism of sucrose in E. coli as conferred by genes from Agrobacterium tumefaciens.
Escherichia coli strains that did not have the ability to use sucrose as a sole carbon source gained this ability after receiving a cloned fragment of DNA from Agrobacterium tumefaciens. No invertase was detected in the sucrose-metabolizing E. coli, but evidence for the activity of certain enzymes, known to be produced by biotype 1 strains of Agrobacterium, were found. Evidence was found for the presence of D-glucoside 3-dehydrogenase (G3DH) and alpha-3-ketoglucosidase. The activity of enzyme extracts on 3-ketosucrose also indicated that 3-ketoglucose reductase, or some enzyme that acts on 3-ketoglucose, was present in the Suc+ E. coli as well. The fragment was found to complement a G3DH mutant of A. tumefaciens and was also found to confer chemotaxis towards sucrose in E. coli.
Schuerman
P L
PL
Department of Pomology, University of California at Davis 95616, USA.
Liu
J S
JS
Mou
H
H
Dandekar
A M
AM
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D002633:Chemotaxis; D004926:Escherichia coli / Q000378:metabolism*; D005798:Genes, Bacterial; D005948:Glucose Dehydrogenases / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*
D006027:Glycosides; D013395:Sucrose; D005948:Glucose Dehydrogenases; C051543:glucoside 3-dehydrogenase