Role of the first round of DNA replication in reprogramming gene expression in the preimplantation mouse embryo.
The first round of DNA replication has been proposed to provide a window of opportunity for maternally-derived transcription factors to gain access to their cognate cis-binding DNA sequences and thereby reprogram the pattern of gene expression that occurs during the 2-cell stage. Using high-resolution, two-dimensional electrophoresis of metabolically radio-labeled polypeptides, we report the expression of a group of several polypeptides whose synthesis in the 2-cell embryo is due to transcription (i.e., alpha-amanitin-sensitive) and depends on the first round of DNA replication (i.e., aphidicolin-sensitive). We also describe the synthesis of another subset of alpha-amanitin-sensitive polypeptides whose expression does not require the first round of DNA replication (i.e., aphidicolin-insensitive). These results are consistent with the proposed role of the first round of DNA replication in reprogramming the pattern of gene expression.
Davis
W
W
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018, USA.
Schultz
R M
RM
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D001755:Blastocyst / Q000378:metabolism*; D002453:Cell Cycle; D004261:DNA Replication; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015053:Zygote / Q000378:metabolism*
D000546:Amanitins; D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D010455:Peptides; D016590:Aphidicolin