Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated.
A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.
Dhankher
O P
OP
Department of Biological Sciences, University of Durham, UK.
Drew
J E
JE
Gatehouse
J A
JA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D018744:DNA, Plant / Q000032:analysis; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017343:Genes, Plant; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D018744:DNA, Plant; D018840:HSP70 Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger