title: Local immunoglobulin G antibodies in the stomach may contribute to immunity against Helicobacter infection in mice.
abstract: OBJECTIVE
  Orogastric immunization of mice with Helicobacter antigens, together with a mucosal adjuvant (cholera toxin), has been shown to confer immunity in the Helicobacter felis infection model. The aim of the study was to investigate the humoral immune responses associated with immunity and to compare these with responses in H. felis-infected mice.
  
  
  METHODS
  Enzyme-linked immunoassays were used to characterize the antibody-secreting cells and antibodies present at mucosal and systemic sites in mice. Animals were immunized orally with either whole-cell H. felis sonicates or Helicobacter pylon urease or heat-shock proteins.
  
  
  RESULTS
  Infection of mice with H. felis preferentially induced the recruitment of plasma cells committed to immunoglobulin (Ig) A synthesis in salivary gland and gastric tissues. Antigen-specific IgA was the major antibody class detected in mucosal secretions recovered from these tissues. In contrast, immunization of mice against H. felis infection induced the proliferation of large numbers of IgG-secreting cells, as well as the synthesis of local IgG antibodies, in the gastric mucosa of the animals. Protection against H. felis infection occurred in the absence of gastric IgA responses in sonicate-immunized mice.
  
  
  CONCLUSIONS
  It is proposed that locally synthesized specific IgG antibodies contribute to immunity against gastric Helicobacter infection.
authors.affiliation: Unité de Pathogénie Bactérienne des Muqueuses, INSERM Unité 389, Institut Pasteur, Paris, France. rferrero@pasteur.fr
authors.forename: R L
authors.initials: RL
authors.lastname: Ferrero
authors.forename: J M
authors.initials: JM
authors.lastname: Thiberge
authors.forename: A
authors.initials: A
authors.lastname: Labigne
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D005753:Gastric Mucosa / Q000276:immunology*; D005756:Gastritis / Q000276:immunology* / Q000382:microbiology*; D016998:Helicobacter / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology*; D007114:Immunization; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G