T cells subsets and cytokines in allergic and non-allergic children. II. Analysis and IL-5 and IL-10 mRNA expression and protein production.
Interleukin 5 (IL-5) has an enhancing effect on IL-4 induced immunoglobulin E (IgE) synthesis. Furthermore, IL-5 plays an important role in the differentiation, recruitment, activation and survival of eosinophils. IL-10 has a downmodulating effect on interferon gamma (IFN-gamma) production and can exert strong anti-inflammatory activities. Therefore, we analysed whether differences were present in IL-5 and IL-10 mRNA expression and protein production between T cells of children with allergic and non-allergic asthma, atopic dermatitis and healthy control children. We demonstrated significant increases in IL-5 mRNA expression and protein production in different T cell fractions of children with allergic and non-allergic asthma and children with atopic dermatitis as compared to healthy controls. This indicates that IL-5 is not only involved in allergy, but also plays a role in the inflammatory process of non-allergic asthma. Interestingly, IL-10 mRNA expression by purified T cells of children with allergic and non-allergic asthma and children with atopic dermatitis was strongly decreased as compared with that of healthy controls. In the peripheral blood mononuclear cell (PBMC) fraction, IL-10 mRNA expression was comparable between the four groups. We hypothesize that this decreased T cell derived IL-10 expression results in a lack of immunosuppression of the inflammatory process in these diseases. However, a role of monocyte derived IL-10 cannot be ruled out.
Koning
H
H
Department of Paediatrics, Sophia Children's Hospital; Rotterdam, The Netherlands.
Neijens
H J
HJ
Baert
M R
MR
Oranje
A P
AP
Savelkoul
H F
HF
D001249:Asthma / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D016022:Case-Control Studies; D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D003876:Dermatitis, Atopic / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D004804:Eosinophils; D015870:Gene Expression; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D066298:In Vitro Techniques; D007223:Infant; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics*; D015848:Interleukin-5 / Q000096:biosynthesis* / Q000235:genetics*; D007958:Leukocyte Count; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism
D016207:Cytokines; D017931:DNA Primers; D015848:Interleukin-5; D012333:RNA, Messenger; D016753:Interleukin-10