In utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin: effects on development of the male and female reproductive system of the mouse.
To evaluate effects of in utero and lactational 2,3,7,8-tetrachlorodibenzo-rho-dioxin (TCDD) exposure on male and female reproductive system development of the mouse, the offspring of pregnant ICR mice administered 0, 15, 30, or 60 microg TCDD/kg on Gestation Day (GD) 14 were examined at the postweanling, pubertal, young adult, and adult stages of development. Dam and offspring body weights and prenatal and postnatal mortality were unaffected by TCDD exposure. The most sensitive endpoints in male offspring were decreased ventral prostate, coagulating gland, and thymus weights, accelerated eye opening, and hydronephrosis. Decreases in pituitary gland weight and epididymal sperm numbers were also found in TCDD-exposed male offspring. Testis, epididymis, and dorsolateral prostate weights, anogenital distance, latencies to testis descent and to preputial separation, and serum testosterone concentrations were unaffected. At the highest maternal TCDD dose uterus weights were decreased in female offspring evaluated during estrus and diestrus. No morphologic changes in the external genitalia of female offspring were found, nor were there alterations in ovary or pituitary gland weights. Cross-species comparisons showed that the mouse was not as sensitive to TCDD-induced developmental reproductive toxicity as the rat and hamster. Many endpoints affected by TCDD in rat and hamster offspring were either not affected or were less sensitive in mouse offspring. Endpoints of androgenic status were not affected in the mouse, decreases in accessory sex organ weights were restricted to fewer organs in the mouse, and decreases in daily sperm production were not found in the mouse. The only developmental reproductive endpoint observed in all three species was a reduction in epididymal sperm numbers.
Theobald
H M
HM
School of Pharmacy, University of Wisconsin, Madison 53706, USA.
Peterson
R E
RE
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D004822:Epididymis / Q000187:drug effects; D004971:Estrus / Q000187:drug effects / Q000502:physiology; D005260:Female; D005835:Genitalia / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D006869:Hydronephrosis / Q000139:chemically induced; D007774:Lactation / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011467:Prostate / Q000187:drug effects; D012098:Reproduction / Q000502:physiology; D012669:Seminal Vesicles / Q000187:drug effects / Q000378:metabolism; D013076:Sperm Count; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000097:blood; D014599:Uterus / Q000187:drug effects / Q000378:metabolism
D000072317:Polychlorinated Dibenzodioxins; D013739:Testosterone