title: Characterization of two newly established cell lines derived from squamous cell carcinomas of the oesophagus.
abstract: This study describes characteristics of two newly established cell lines (OSC-1 and OSC-2), derived from two oesophageal squamous cell carcinomas. Morphologically, OSC-1 cells and OSC-2 cells grew in epithelial cobblestone patterns with cells piling up to 4 cells. Ultrastructurally, both cell lines showed formation of desmosomes; however, tonofilaments were only formed by OSC-2 cells. Immunohistochemical investigations revealed coexpression of vimentin and cytokeratin in OSC-1 cells and OSC-2 cells. A cytokeratin subtype typical for mature squamous epithelia (cytokeratin 13) was expressed only in OSC-2 cells. OSC-1 cells showed tumour formation in nude mice, whereas OSC-2 cells did not. Cytogenetic analysis revealed that OSC-1 cells had a hyperdiploid karyotype and OSC-2 cells had a near-triploid karyotype. In both cell lines, the formation of multicellular spheroids could be induced. In conclusion, in comparison with OSC-2 cells the OSC-1 cells were characterized by a poorer degree of differentiation and by a more aggressive growth behaviour in vivo.
authors.affiliation: Institute of Pathology, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Sarbia
authors.forename: N
authors.initials: N
authors.lastname: Bösing
authors.forename: B
authors.initials: B
authors.lastname: Hildebrandt
authors.forename: P
authors.initials: P
authors.lastname: Koldovsky
authors.forename: C D
authors.initials: CD
authors.lastname: Gerharz
authors.forename: H E
authors.initials: HE
authors.lastname: Gabbert
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle; D002460:Cell Line; D002871:Chromosome Banding; D003896:Desmosomes / Q000473:pathology / Q000648:ultrastructure; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis; D008677:Metaphase; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis / Q000096:biosynthesis
chemical_list: D014746:Vimentin; D007633:Keratins