title: Expression of adhesion molecules on mononuclear cells from individuals with stable atopic asthma.
abstract: Activated mononuclear cells (lymphocytes and monocytes) (MNCs) circulate in peripheral blood and accumulate in the airways of individuals with steady-state asthma. The expression of adhesion molecules on MNCs of 10 patients with steady-state atopic asthma and 10 non-atopic control subjects was measured by flow cytometry. The mean fluorescence intensity of CD23 (P = 0.0003), CD11a (P = 0.034), and very late antigen-4 (VLA-4) (P = 0.016) was increased on lymphocytes of asthmatic patients relative to those of controls. Although the expression of CD16 (P = 0.002) and CD23 (P = 0.002), which are associated with differentiation into macrophages, was increased on monocytes of patients relative to those of controls, monocyte expression of VLA-4 (P = 0.006) and sialyl Lewis x (P = 0.005) was reduced. The concentration of interleukin-4 (IL-4) in serum was significantly higher in asthmatic patients than in normal subjects (P = 0.023), and a significant correlation was apparent between the serum concentration of IL-4 and the expression of VLA-4 on lymphocytes (p = 0.71, P = 0.03) or on monocytes (p = -0.72, P = 0.03) in asthmatic patients. Results suggest that IL-4 may contribute to the priming of adhesion molecule expression on lymphocytes even in steady-state conditions in individuals with chronic allergic inflammation.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medicine, Tottori University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tomita
authors.forename: T
authors.initials: T
authors.lastname: Tanigawa
authors.forename: H
authors.initials: H
authors.lastname: Yajima
authors.forename: H
authors.initials: H
authors.lastname: Sano
authors.forename: K
authors.initials: K
authors.lastname: Fukutani
authors.forename: Y
authors.initials: Y
authors.lastname: Hitsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000097:blood*; D018845:CD11 Antigens / Q000097:blood; D018791:CD4 Lymphocyte Count; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D017455:Receptors, IgE / Q000097:blood; D017452:Receptors, IgG / Q000097:blood
chemical_list: D018845:CD11 Antigens; D015815:Cell Adhesion Molecules; D017455:Receptors, IgE; D017452:Receptors, IgG; D015847:Interleukin-4