Flow cytometric analysis of major histocompatibility complex (MHC) class II antigen expression on brain cells from Borna disease virus-infected rats without an intervening in vitro culture step.
Borna disease virus (BDV) infects astrocytes in the Lewis rat brain. BDV-infected astrocytes have been shown to express MHC class II in vitro but not in vivo. Using a sensitive fluorescence-activated cytometric technique, we now report the detection of MHC class II on freshly harvested S100-positive cells from BDV-infected rat brain, without an intervening in vitro culture step. These data support the hypothesis that astrocytes from BDV-infected rats express MHC class II on their surface and, thus, are potential participants in the encephalitic response to BDV infection.
Rubin
S A
SA
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21218, USA.
Park
S W
SW
Soloski
M J
MJ
Carbone
K M
KM
D000818:Animals; D001253:Astrocytes / Q000473:pathology*; D001890:Borna Disease / Q000276:immunology* / Q000473:pathology; D001891:Borna disease virus / Q000276:immunology; D001921:Brain / Q000276:immunology* / Q000473:pathology; D018792:Encephalitis, Viral / Q000276:immunology* / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000096:biosynthesis*; D007150:Immunohistochemistry / Q000379:methods; D051381:Rats; D009418:S100 Proteins / Q000032:analysis / Q000096:biosynthesis; D012680:Sensitivity and Specificity
D000949:Histocompatibility Antigens Class II; D009418:S100 Proteins