Finasteride in biological fluids: extraction and separation by a graphitized carbon black cartridge and quantification by high-performance liquid chromatography.
A simple, specific and sensitive high-performance liquid chromatographic method has been developed and validated for the determination of finasteride in human plasma. A solid-phase extraction procedure was used to isolate finasteride from the biological matrix before quantitative analysis. The analyte was separated on a Symmetry reversed-phase column using acetonitrile-0.04 M orthophosphoric acid (pH 4.0) as mobile phase and quantified by measuring its UV absorbance at 215 nm. The limit of detection for the analyte was 0.005 microg/ml. 4-Androstene-3,17-dione was used as internal standard. The calibration graph of the method was linear from 0.01 to 3.0 microg/ml of finasteride in human plasma, and the coefficient of variation less than 4.5%. This HPLC procedure is simple, precise and accurate for the determination of finasteride in human plasma.
Carlucci
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Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università dell'Aquila, Coppito (L'Aquila), Italy.
Mazzeo
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D002244:Carbon; D042944:Cholestenone 5 alpha-Reductase; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000097:blood*; D018120:Finasteride / Q000097:blood*; D006801:Humans; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D004791:Enzyme Inhibitors; D018120:Finasteride; D002244:Carbon; D010088:Oxidoreductases; D042944:Cholestenone 5 alpha-Reductase