Chromatography of crotamiton and its application to the determination of active ingredients in ointments.
Crotamiton, which is a mixture of cis and trans isomers, was investigated by several separation techniques. One of the HPLC modes, in which crotamiton eluted as a single peak, was selected for the determination of five active ingredients (crotamiton, prednisolone, glycyrrhetinic acid, dibucaine and chlorhexidine hydrochloride) in an ointment. The simultaneous determination was performed using isocratic reversed-phase mode within 20 min by employing an octyl (C8) column and a mobile phase containing sodium dodecyl sulfate (SDS) and 2-propanol. The method was successfully applied to quality control and stability testing of the ointment.
Izumoto
S
S
Analytical Research Laboratory, Tanabe Seiyaku, Osaka, Japan.
Machida
Y
Y
Nishi
H
H
Nakamura
K
K
Nakai
H
H
Sato
T
T
D000982:Antipruritics / Q000032:analysis*; D002710:Chlorhexidine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003992:Dibucaine / Q000032:analysis; D004355:Drug Stability; D006034:Glycyrrhetinic Acid / Q000032:analysis; D007306:Insecticides / Q000032:analysis*; D015394:Molecular Structure; D009824:Ointments; D011239:Prednisolone / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D013237:Stereoisomerism; D014052:Toluidines / Q000032:analysis*
D000982:Antipruritics; D007306:Insecticides; D009824:Ointments; D014052:Toluidines; D011239:Prednisolone; C005553:crotamiton; D003992:Dibucaine; D006034:Glycyrrhetinic Acid; D002710:Chlorhexidine