Reproducibility of vitreous fluorophotometry in patients with type 1 diabetes mellitus.
We assessed the day-to-day reproducible of vitreous fluorophotometry in 7 type 1 diabetic patients and in 1 healthy control subject. The coefficient of variation for duplicate measurements was 33.7, 40.3 and 23.6%, for the right eyes, the left eyes and mean values of both eyes, respectively. Assessment of reproducibility in 1 healthy subject, measured 4 times, resulted in coefficients of variation of about 60%. These values are somewhat worse than those obtained by others in healthy subjects. Since technical problems do not seem to play a significant role in this regard, our reproducibility results reflect the true biological variability of the permeability of the blood-retina barrier assessed by vitreous fluorophotometry in diabetic patients. This variability is substantial. Therefore, we conclude that this method is not precise enough to be used in intervention studies with a limited number of subjects.
Jager
J
J
St. Lucas Ziekenhuis, Winschoten.
Tromp
A
A
Hooymans
J M
JM
Reitsma
W D
WD
Smit
A J
AJ
D000328:Adult; D000368:Aged; D001813:Blood-Retinal Barrier; D015925:Cryopreservation; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins / Q000493:pharmacokinetics; D015590:Fluorophotometry; D005615:Freezing; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D014822:Vitreous Body / Q000378:metabolism*
D005452:Fluoresceins; D019793:Fluorescein