title: Conformational stability of a type II' beta-turn motif in human growth hormone [6-13] peptide analogues at hydrophobic surfaces.
abstract: The interactive properties of several peptides related to human growth hormone (hGH) [6-13] containing a type II' beta-turn motif have been investigated using reversed-phase high-performance liquid chromatography (RP-HPLC). Various chromatographic parameters related to the hydrophobic interactive surface area and binding affinity were measured over the range of temperatures between 5 and 85 degrees C. Variations in these parameters were consistent with significant differences in the relative stability of the type II' beta-turn structures of these peptidomimetics. The effect of changes in peptide conformation were also investigated through the analysis of band-broadening behaviour during the chromatographic process. Significant variations in bandwidth observed at discrete temperatures were related to the rate of interconversion between the type II' beta-turn and more extended conformers. These investigations further document the potential of RP-HPLC for monitoring subtle changes in peptide secondary structure at hydrophobic interfaces.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: T H
authors.initials: TH
authors.lastname: Lee
authors.forename: P E
authors.initials: PE
authors.lastname: Thompson
authors.forename: M T
authors.initials: MT
authors.lastname: Hearn
authors.forename: M I
authors.initials: MI
authors.lastname: Aguilar
mesh_terms: D002851:Chromatography, High Pressure Liquid; D013006:Growth Hormone / Q000737:chemistry*; D019382:Human Growth Hormone / Q000031:analogs & derivatives* / Q000737:chemistry*; D006801:Humans; D008961:Models, Structural; D010446:Peptide Fragments / Q000737:chemistry*; D017433:Protein Structure, Secondary; D013816:Thermodynamics
chemical_list: D010446:Peptide Fragments; D019382:Human Growth Hormone; C037849:somatotropin (6-13); D013006:Growth Hormone