In vitro and in vivo studies of murine sarcoma cells after prolonged treatment with promoting phorbol ester TPA.
Murine sarcoma cell line (L-1) treated with promoting phorbol ester (TPA) showed decreased content and activity of protein kinase C (PKC) as measured by Western blotting and histone phosphorylation methods. The PKC depleted line (L-1R) produced bigger, tumors after s.c. transplantation into syngeneic mice and more spontaneous and artificial metastases developing after i.v. injection of tumor cells. The in vitro studies revealed decreased: adhesiveness, migratory and invasiveness properties of PKC depleted cells. Negative correlation between in vitro and in vivo studies were found.
Janik
P
P
Department of Cell Biology, Cancer Center Institute of Oncology, Warsaw, Poland.
Woynarowska
B
B
Miłoszewska
J
J
Szaniawska
B
B
D000818:Animals; D015153:Blotting, Western; D002273:Carcinogens / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D005260:Female; D008175:Lung Neoplasms / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010766:Phosphorylation; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002273:Carcinogens; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate