Cloning of the pumpkin ascorbate oxidase gene and analysis of a cis-acting region involved in induction by auxin.
A genomic clone encoding ascorbate oxidase was isolated from pumpkin (Cucurbita sp.). This gene is consisted of four exons and three introns. Analyses of the promoter fusion to beta-glucuronidase reporter gene by transient expression assay in pumpkin fruit tissues suggested the existence of a cis-acting region responsible for auxin regulation.
Kisu
Y
Y
Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan.
Harada
Y
Y
Goto
M
M
Esaka
M
M
D000595:Amino Acid Sequence; D001204:Ascorbate Oxidase / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D005091:Exons; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D015698:Genomic Library; D005966:Glucuronidase / Q000235:genetics; D007210:Indoleacetic Acids / Q000494:pharmacology*; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014675:Vegetables / Q000201:enzymology / Q000235:genetics*
D007210:Indoleacetic Acids; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D001204:Ascorbate Oxidase; D005966:Glucuronidase