A scanning electron microscopy study of disturbances in the developing rat molar induced by cyclophosphamide.
Scanning electron microscopy was used to study the effect of cyclophosphamide (Cy) on molar development in 18 Sprague-Dawley rats from 15 to 48 days of age after birth. Doses of 30 mg/kg body weight of Cy dissolved in 1 ml 0.9% NaCl were given to the rats at 10 and 13 days of age. Eighteen control rats had injections of 1 ml 0.9% NaCl at the same ages. The most obvious changes in the experimental teeth were found in the developing roots of the first and second molars and in both the crown and roots of the third molar. The roots of the first and second molars were short and showed apical closure in the experimental rats. In addition to the disturbances in crown and root formation, the third molars were also significantly reduced in total size as compared with the third molars in the control rats.
Näsman
M
M
Department of Orthodontics, Karolinska Institutet, and Clinical Research Center, Huddinge Hospital, Sweden.
Hultenby
K
K
Forsberg
C M
CM
D000505:Alopecia / Q000139:chemically induced; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000494:pharmacology*; D003743:Dental Enamel / Q000187:drug effects / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D007274:Injections, Intraperitoneal; D008297:Male; D008855:Microscopy, Electron, Scanning; D008963:Molar / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D008964:Molar, Third / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D009805:Odontogenesis / Q000187:drug effects*; D009811:Odontometry; D051381:Rats; D017207:Rats, Sprague-Dawley; D019227:Tooth Apex / Q000187:drug effects / Q000648:ultrastructure; D019237:Tooth Cervix / Q000187:drug effects / Q000648:ultrastructure; D019228:Tooth Crown / Q000187:drug effects / Q000648:ultrastructure; D014092:Tooth Root / Q000187:drug effects / Q000648:ultrastructure
D018906:Antineoplastic Agents, Alkylating; D003520:Cyclophosphamide