title: Method of measurement of pancreatic elastase II activity and postnatal development of proteases in human duodenal juice and bovine and porcine pancreatic tissue.
abstract: A specific method for pancreatic elastase II activity analysis was developed. True elastase II activity could be discriminated from that of elastase I and chymotrypsin. The postnatal development of four pancreatic proteases in the duodenal juice of children and in the pancreatic homogenates of calves and piglets was measured. The study was carried out on patients without (14 children) and with (5 children) pancreatic insufficiency. Calves and piglets were either milk-fed or weaned until slaughter at different ages. Profiles of enzyme development were globally similar in milk-fed piglets and calves, while in children without pancreatic insufficiency, no significant change was observed between 4 and 168 months. In children with pancreatic insufficiency, enzyme activity was low. In animals, elastase II and chymotrypsin activities were maximal at birth, decreased with age, and probably were associated with the digestion of milk protein. In contrast, elastase I and trypsin activities increased markedly after weaning in connection with the intake of solid food.
authors.affiliation: Laboratoire du Jeune Ruminant, I.N.R.A., Rennes, France.
authors.forename: M
authors.initials: M
authors.lastname: Gestin
authors.forename: I
authors.initials: I
authors.lastname: Le Huerou-Luron
authors.forename: J
authors.initials: J
authors.lastname: Peiniau
authors.forename: E
authors.initials: E
authors.lastname: Thioulouse
authors.forename: C
authors.initials: C
authors.lastname: Desbois
authors.forename: G
authors.initials: G
authors.lastname: Le Drean
authors.forename: D
authors.initials: D
authors.lastname: Feldman
authors.forename: A
authors.initials: A
authors.lastname: Aumaitre
authors.forename: P
authors.initials: P
authors.lastname: Guilloteau
mesh_terms: D000293:Adolescent; D000818:Animals; D016022:Case-Control Studies; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D002918:Chymotrypsin / Q000378:metabolism*; D004386:Duodenum / Q000201:enzymology*; D010188:Exocrine Pancreatic Insufficiency / Q000201:enzymology*; D006801:Humans; D007223:Infant; D007419:Intestinal Secretions / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000378:metabolism*; D013552:Swine; D014357:Trypsin / Q000378:metabolism*
chemical_list: D012697:Serine Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; C117309:pancreatic elastase II