title: A 2.8-Mb clone contig of the multiple endocrine neoplasia type 1 (MEN1) region at 11q13.
abstract: Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder that results in parathyroid, anterior pituitary, and pancreatic and duodenal endocrine tumors in affected individuals. The MEN1 locus is tightly linked to the marker PYGM on chromosome 11q13, and linkage analysis has placed the MEN1 gene within a 2-Mb interval flanked by D11S1883 and D11S449. As a step toward cloning the MEN1 gene, we have constructed a 2.8-Mb clone contig consisting of YAC and bacterial clones (PAC, BAC, and P1) for the D11S480 to D11S913 region. The bacterial clones alone represent nearly all of the 2.8-Mb contig. The contig was assembled based on a high-density STS-content analysis of 79 genomic clones (YAC, PAC, BAC, and P1) with 118 STSs. The STSs included 22 polymorphic markers and 20 transcripts, with the remainder primarily derived from the end sequences of the genomic clones. An independent cosmid contig for the 1-Mb PYGM-SEA region was also generated. Support for correctness of the 2.8-Mb contig map comes from an independent ordering of the clones by fiber-FISH. This sequence-ready contig will be a useful resource for positional cloning of MEN1 and other disease genes whose loci fall within this region.
authors.affiliation: Laboratory of Gene Transfer, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Guru
authors.forename: S E
authors.initials: SE
authors.lastname: Olufemi
authors.forename: P
authors.initials: P
authors.lastname: Manickam
authors.forename: C
authors.initials: C
authors.lastname: Cummings
authors.forename: L M
authors.initials: LM
authors.lastname: Gieser
authors.forename: B L
authors.initials: BL
authors.lastname: Pike
authors.forename: M L
authors.initials: ML
authors.lastname: Bittner
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: A C
authors.initials: AC
authors.lastname: Chinault
authors.forename: N J
authors.initials: NJ
authors.lastname: Nowak
authors.forename: A
authors.initials: A
authors.lastname: Brzozowska
authors.forename: J S
authors.initials: JS
authors.lastname: Crabtree
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: B A
authors.initials: BA
authors.lastname: Roe
authors.forename: J M
authors.initials: JM
authors.lastname: Weisemann
authors.forename: M S
authors.initials: MS
authors.lastname: Boguski
authors.forename: S K
authors.initials: SK
authors.lastname: Agarwal
authors.forename: A L
authors.initials: AL
authors.lastname: Burns
authors.forename: A M
authors.initials: AM
authors.lastname: Spiegel
authors.forename: S J
authors.initials: SJ
authors.lastname: Marx
authors.forename: W L
authors.initials: WL
authors.lastname: Flejter
authors.forename: P J
authors.initials: PJ
authors.lastname: de Jong
authors.forename: F S
authors.initials: FS
authors.lastname: Collins
authors.forename: S C
authors.initials: SC
authors.lastname: Chandrasekharappa
mesh_terms: D018244:Chromosomes, Artificial, Yeast; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D003360:Cosmids; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D009377:Multiple Endocrine Neoplasia / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D016324:Sequence Tagged Sites
chemical_list: C105373:MEN1 protein, human; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins