title: High concentration of recombinant murine interferon-beta enhances the growth of Orientia (formerly Rickettsia) tsutsugamushi Gilliam in mouse L929 cells.
abstract: We studied the effect of recombinant murine interferons (rMuIFNs) on the growth of Orientia (formerly Rickettsia) tsutsugamushi Gilliam in mouse L929 cells. Rickettsial growth was measured by flow cytometry. rMUIFN-gamma inhibited the growth of O. tsutsugamushi at the concentrations of 100 i.u./ml and 1,000 i.u./ml in accord with previous reports. Relatively low concentrations (10 i.u./ml and 100 i.u./ml) of rMUIFN-beta also inhibited the growth of O. tsutsugamushi. On the other hand, high concentrations (1,000 i.u./ml and 10,000 i.u./ml) of rMuIFN-beta enhanced the growth of the rickettsia. This enhancement of rickettsial growth was blocked by anti-murine IFN-beta monoclonal antibody (MoAb). rMuIFN-beta also enhanced the growth of Rickettsia sibirica 246 in L929 cells to some extent.
authors.affiliation: Department of Bacteriology, Faculty of Medicine, Kagoshima University, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Tasneen
authors.forename: K
authors.initials: K
authors.lastname: Yoshiie
authors.forename: T
authors.initials: T
authors.lastname: Ohkawa
authors.forename: H
authors.initials: H
authors.lastname: Oda
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D016899:Interferon-beta / Q000008:administration & dosage*; D007371:Interferon-gamma / Q000008:administration & dosage; D007739:L Cells; D051379:Mice; D012285:Orientia tsutsugamushi / Q000254:growth & development*; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; D016899:Interferon-beta; D007371:Interferon-gamma