Specific antibodies to Trypanosoma cruzi among blood donors in Los Angeles, California.
BACKGROUND
Trypanosoma cruzi, the cause of Chagas' disease, is often transmitted by transfusion in Latin America. Previous studies showed that at least 1 in 1000 eligible blood donors at the Los Angeles County+University of Southern California (LAC+USC) Medical Center Blood Bank had specific antibodies to T. cruzi. In June 1993, serologic screening of prospective allogeneic donors at epidemiologic risk for T. cruzi infection was begun voluntarily.


METHODS
The risk of T. cruzi infection in all eligible donors was assessed by questionnaire. At-risk donors were screened serologically for antibodies to T. cruzi with an enzyme immunoassay, and confirmatory testing was done with a radioimmunoprecipitation assay.


RESULTS
During the 29-month study period 1311 (39.5%) of 3320 donors were judged to be at risk for T. cruzi infection. Seven donors (1/475) were reactive by an enzyme immunoassay, and six of these seven (1/ 553) were positive in a radioimmunoprecipitation assay. All radioimmunoprecipitation assay-positive donors had been born in countries in which Chagas' disease is endemic. One person in this group had received a transfusion in his homeland.


CONCLUSIONS
These results demonstrate that a substantive proportion of eligible blood donors at our institution have antibodies specific for T. cruzi and that a commercially available assay can be used to detect these antibodies. Our data suggest that the risk of transmission of T. cruzi by transfusion could be eliminated by serologic testing limited to persons born in or transfused in countries in which Chagas' disease is endemic.
Shulman
I A
IA
Department of Pathology, Los Angeles County+University of Southern California Hospital, USA.
Appleman
M D
MD
Saxena
S
S
Hiti
A L
AL
Kirchhoff
L V
LV
D000328:Adult; D000368:Aged; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology*; D000918:Antibody Specificity; D001782:Blood Donors / Q000706:statistics & numerical data*; D014355:Chagas Disease / Q000097:blood / Q000453:epidemiology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D015141:Los Angeles / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D014349:Trypanosoma cruzi / Q000276:immunology*
D000913:Antibodies, Protozoan