title: Binding of human peripheral blood polymorphonuclear leukocytes to E-selectin (CD62E) does not promote their activation.
abstract: E-selectin (CD62E) is a cytokine-inducible endothelial cell adhesion molecule that tethers polymorphonuclear leukocytes (PMNs) and supports PMN rolling under conditions of flow. We examined whether interaction of PMNs with E-selectin also leads to activation of CD11b/CD18 (Mac-1, alphaMbeta2), an event that can promote firm adhesion. PMNs were added to monolayers of IL-1beta-activated HUVECs and Chinese hamster ovary (CHO) cells transfected with E-selectin cDNA. PMN activation was assessed by 1) increased CD11b/CD18 surface expression, 2) appearance of activation epitope on CD11b/CD18 (CD11b*) detected by mAb CBRM1/5, and 3) decreased L-selectin (CD62L) expression, as determined by flow cytometry. Both adherent and nonadherent supernatant PMNs became activated on IL-1beta-pretreated HUVECs. This activation was not affected by CD62E-blocking mAb P6E2. The activation state of PMNs adhered to CHO cells transfected with E-selectin cDNA was not increased over background and was similar to that of PMNs exposed to parent CHO cells. The findings were confirmed using confocal microscopy, which allowed staining of the cells for CD11b* in situ. In concert, the results suggest that PMN binding to E-selectin does not elicit inter-receptor signaling that could result in strengthening of PMN adhesion to endothelium.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195, USA. herepo@cc.helsinki.fi
authors.forename: H
authors.initials: H
authors.lastname: Repo
authors.forename: Y P
authors.initials: YP
authors.lastname: Rochon
authors.forename: B R
authors.initials: BR
authors.lastname: Schwartz
authors.forename: S R
authors.initials: SR
authors.lastname: Sharar
authors.forename: R K
authors.initials: RK
authors.lastname: Winn
authors.forename: J M
authors.initials: JM
authors.lastname: Harlan
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000276:immunology; D006224:Cricetinae; D019040:E-Selectin / Q000276:immunology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D018375:Neutrophil Activation / Q000276:immunology*; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*
chemical_list: D019040:E-Selectin