Postischemic expression of P-selectin immunoreactivity in rat brain.
Change of immunoreactive P-selectin was examined in rat brain after transient middle cerebral artery (MCA) occlusion (O) with anti-P-selectin monoclonal antibody using brain samples of sham control and after ischemia. Temporal, spatial, and cellular changes of immunohistochemical expressions of P-selectin were evaluated with rat brain sections at 2 and 8 h, 1, 3, and 7 days of reperfusion after 1 h of MCAO. Western blot showed a single band at molecular weight of 140 kDa for P-selectin after ischemia. P-selectin immunoreactivity was not normally present in rat brain sections. However, it was expressed mainly in the post-capillary venules of the cerebral cortex and caudate in the MCA territory with a peak at 8 h-1 day. The expression was diminished by 3 days of reperfusion. The present results indicate that P-selectin was expressed from an earlier stage of reperfusion in post-capillary venules, and the expression became maximum at the same time both in the cerebral cortex and caudate.
Suzuki
H
H
Department of Neurology, Tohoku University School of Medicine, Tohoku Kouseinenkin Hospital, Aobaku, Sendai, Japan.
Abe
K
K
Tojo
S
S
Morooka
S
S
Kimura
K
K
Mizugaki
M
M
Itoyama
Y
Y
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry / Q000502:physiology; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002536:Cerebral Arteries / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D008970:Molecular Weight; D019007:P-Selectin / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D019007:P-Selectin