title: Synthesis of isopropyl-1-thio-beta-D-glucopyranoside (IPTGlc), an inducer of Aspergillus niger B1 beta-glucosidase production.
abstract: Production of beta-glucosidase in Aspergillus niger B1 is subjected to catabolic repression by glucose. Aspergillus niger B1 grown on bran as a carbon source secreted beta-glucosidase. The maximum level of the enzyme was reached after 7 d of fermentation. Addition of 1% glucose to the medium suppressed beta-glucosidase production to undetectable levels. In this study, the organic synthesis of a potential inducer of beta-glucosidase production by A. niger B1's reported. Isopropyl-1-thio-beta-D-glucopyranoside (IPTGlc) was synthesized using a two-step organic synthesis protocol. The H-NMR data agreed with those reported previously for the galactoside analog. When IPTGlc was added 24 h after inoculation at a final concentration of 0.4 mM, similar levels of beta-glucosidase were reached 3 to 4 d earlier as compared to fermentation without IPTGlc induction. In practice, this may translate to a more efficient method of producing beta-glucosidase from this fungus.
authors.affiliation: Kennedy-Leigh Center for Horticultural Research, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Birk
authors.forename: A
authors.initials: A
authors.lastname: Ikan
authors.forename: B
authors.initials: B
authors.lastname: Bravdo
authors.forename: S
authors.initials: S
authors.lastname: Braun
authors.forename: O
authors.initials: O
authors.lastname: Shoseyov
mesh_terms: D001234:Aspergillus niger / Q000187:drug effects / Q000201:enzymology*; D004790:Enzyme Induction; D005947:Glucose / Q000494:pharmacology; D007544:Isopropyl Thiogalactoside / Q000138:chemical synthesis* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D001617:beta-Glucosidase / Q000096:biosynthesis*
chemical_list: D007544:Isopropyl Thiogalactoside; D001617:beta-Glucosidase; D005947:Glucose