title: Morphological changes of intraparenchymal arterioles after experimental subarachnoid hemorrhage in dogs.
abstract: OBJECTIVE
  Morphological and microcirculatory changes in intraparenchymal vessels after subarachnoid hemorrhage (SAH) have not yet been fully clarified. We conducted this experimental study to investigate the serial morphological changes of intraparenchymal arterioles after SAH.
  
  
  METHODS
  SAH was produced by injecting autologous arterial blood into the cisterna magna twice at 48-hour intervals in 30 dogs. The dogs were killed 3, 7, or 14 days after SAH, and then perfusion-fixed specimens of both anterior sylvian giri were obtained by using two methods. Microvascular corrosion casts produced by arterial injection of polyester resin were examined using scanning electron microscopy, and the widths of 40 arterioles of each animal were measured. Sectioned slices from the brain surface to 500 microns deep were examined by light microscopy, and external diameter, internal diameters, and wall thickness of the arterioles at depths of 50, 200, and 500 microns from the brain surface were morphometrically evaluated in 40 arterioles of each animal. In control animals receiving cisternal injections of mock cerebrospinal fluid (n = 10) and in healthy control animals (n = 10), the same examination and evaluation were performed.
  
  
  RESULTS
  Corrosion casts of arterioles showed tapered narrowing with folding after SAH, and the width of the arterioles significantly decreased 3 and 7 days after SAH (P < 0.01). Morphometric examination by light microscopy showed a significant decrease of internal diameter of arterioles associated with a significant increase of wall thickness at any depth from the brain surface 3 and 7 days after SAH (P < 0.05 or P < 0.01). These findings improved 14 days after SAH. Control animals receiving cisternal injections of mock cerebrospinal fluid showed no significant differences compared with healthy control animals.
  
  
  CONCLUSIONS
  These results suggest that constriction of intraparenchymal arterioles occurs after SAH and may contribute to delayed cerebral ischemia.
authors.affiliation: Department of Neurosurgery, Hirosaki University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ohkuma
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Shibata
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001160:Arterioles / Q000473:pathology; D001921:Brain / Q000098:blood supply*; D016319:Corrosion Casting; D004285:Dogs; D005260:Female; D002546:Ischemic Attack, Transient / Q000473:pathology*; D008297:Male; D008833:Microcirculation / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D013345:Subarachnoid Hemorrhage / Q000473:pathology*; D014661:Vasoconstriction / Q000502:physiology