title: Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density-gradient centrifugation.
abstract: A method for isolation of enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of experimentally infected cats was developed using Percoll density-gradient centrifugation. Gamonts and merozoites were obtained essentially free of host-cell debris. A recovery rate of nearly 30% of the parasites in the original preparations was obtained by this method. Merozoites were separated from gamonts by filtration through a 3-micron polycarbonate filter.
authors.affiliation: Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Omata
authors.forename: A
authors.initials: A
authors.lastname: Taka
authors.forename: K
authors.initials: K
authors.lastname: Terada
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: M
authors.initials: M
authors.lastname: Kanda
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000469:parasitology*; D002415:Cats; D002499:Centrifugation, Density Gradient; D005260:Female; D007413:Intestinal Mucosa / Q000469:parasitology*; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D014122:Toxoplasma / Q000254:growth & development / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000469:parasitology*
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone