Minisequencing: a specific tool for DNA analysis and diagnostics on oligonucleotide arrays.
We describe a method for multiplex detection of mutations in which the solid-phase minisequencing principle is applied to an oligonucleotide array format. The mutations are detected by extending immobilized primers that anneal to their template sequences immediately adjacent to the mutant nucleotide positions with single labeled dideoxynucleoside triphosphates using a DNA polymerase. The arrays were prepared by coupling one primer per mutation to be detected on a small glass area. Genomic fragments spanning nine disease mutations, which were selected as targets for the assay, were amplified in multiplex PCR reactions and used as templates for the minisequencing reactions on the primer array. The genotypes of homozygous and heterozygous genomic DNA samples were unequivocally defined at each analyzed nucleotide position by the highly specific primer extension reaction. In a comparison to hybridization with immobilized allele-specific probes in the same assay format, the power of discrimination between homozygous and heterozygous genotypes was one order of magnitude higher using the minisequencing method. Therefore, single-nucleotide primer extension is a promising principle for future high-throughput mutation detection and genotyping using high density DNA-chip technology.
Pastinen
T
T
Department of Human Molecular Genetics, National Public Health Institute, Helsinki, Finland.
Kurg
A
A
Metspalu
A
A
Peltonen
L
L
Syvänen
A C
AC
D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D006580:Genetic Carrier Screening / Q000379:methods*; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods
D015345:Oligonucleotide Probes; C034477:RNA primers; D012313:RNA; D004259:DNA-Directed DNA Polymerase