Labeling proteins at high specific activity using N-succinimidyl 4-[18F](fluoromethyl) benzoate.
High effective specific activity N-succinimidyl 4-[18F](fluoromethyl)benzoate was prepared using a reversed phase HPLC procedure. Reversed phase HPLC removed several additional impurities not removed by normal phase HPLC, thereby increasing the effective specific activity. Small amount (< 100 micrograms) of sensitive proteins such as erythropoietin can be labeled with this reagent in high yield without aggregation.
Lang
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Positron Emission Tomography Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.
Eckelman
W C
WC
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification; D001565:Benzoates / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004921:Erythropoietin / Q000737:chemistry / Q000302:isolation & purification; D005462:Fluorine Radioisotopes; D007202:Indicators and Reagents; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013388:Succinimides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D014168:Transferrin / Q000737:chemistry / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D001565:Benzoates; D005462:Fluorine Radioisotopes; D007202:Indicators and Reagents; C092711:N-succinimidyl 4-(fluoromethyl)benzoate; D011506:Proteins; D013388:Succinimides; D014168:Transferrin; D004921:Erythropoietin