[Acceleration of hematopoietic reconstitution in mice transplanted with syngeneic bone marrow cells by IL-6 gene therapy].
In the present study, the potential of fibroblast-mediated IL-6 gene therapy for accelerating hematopoietic reconstitution after syngeneic bone marrow transplantation (BMT) in BALB/c mice subjected to total body irradiation at a dose of 7 Gy was investigated. We observed that not only recovery of platelets, WBCs, CFU-GM or CFU-MK in bone marrow in mice treated with IL-6 gene therapy in combination with transplantation of 10(7) syngeneic bone marrow cells was faster than that in mice treated with IL-6 gene therapy in combination with transplantation of 10(6) or 10(5) syngeneic bone marrow cells, but also the number of CFU-S or survival rate in mice treated with IL-6 gene therapy in combination with transplantation of 10(7) syngeneic bone marrow cells was significantly higher. Nevertheless, the number of platelets, WBCs, CFU-GM or CFU-MK in bone marrow, CFU-S and survival rate in mice treated with IL-6 gene therapy in combination with transplantation of syngeneic bone marrow cells were elevated more significantly than that in mice transplanted with syngeneic bone marrow cells alone. These data demonstrated that IL-6 gene therapy could markedly augment hematopoietic reconstitution after syngeneic bone marrow transplantation and the more bone marrow cells transplanted, the better was the effect.
Cai
R
R
Department of Immunology, Second Military Medical University, Shanghai.
Cao
X
X
Ma
S
S
D016475:3T3 Cells; D000818:Animals; D016026:Bone Marrow Transplantation; D005796:Genes; D015316:Genetic Therapy; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D015850:Interleukin-6 / Q000235:genetics*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010976:Platelet Count; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000628:therapy*; D014916:Whole-Body Irradiation
D015850:Interleukin-6