title: Determination of heterocyclic aromatic amines in food products: automation of the sample preparation method prior to HPLC and HPLC-MS quantification.
abstract: Heat-processing protein-rich foods may cause the formation of heterocyclic aromatic amines (HAAs), all of which have mutagenic and some also carcinogenic potential. Accurately measuring HAA levels in food products is therefore a necessary to realistically assess this risk factor. A solid-phase extraction method for quantitative HAA analysis has been developed by us over the last few years. This method has recently been automated using a robotic workstation and now allows almost unattended sample preparation, a process which saves a human operator about five hours of benchwork. Cleaned-up samples were analyzed by high performance liquid chromatography (HPLC) and ultraviolet (UV) or mass spectrometric (MS) detection. While HPLC-UV remains the daily tool to quantify HAAs, we found HPLC-electrospray-MS to be an alternative detection method with unique advantages, suited for both HAA identification and quantification.
authors.affiliation: Nestec Ltd, Nestlé Research Centre, Lausanne, Switzerland. laurent.fay@.chlsnr.nestrd.ch
authors.forename: L B
authors.initials: LB
authors.lastname: Fay
authors.forename: S
authors.initials: S
authors.lastname: Ali
authors.forename: G A
authors.initials: GA
authors.lastname: Gross
mesh_terms: D000588:Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D006571:Heterocyclic Compounds / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D012371:Robotics
chemical_list: D000588:Amines; D006571:Heterocyclic Compounds