Identification of an Ixodes ricinus salivary gland fraction through its ability to stimulate CD4 T cells present in BALB/c mice lymph nodes draining the tick fixation site.
BALB/c mice infested with larvae or nymphs of Ixodes ricinus develop in their lymph nodes a T cell-specific immune response triggered by salivary gland soluble antigens (SGA). SGA are apparently conserved in the 3 biological stages of I. ricinus ticks and are species specific. SGA derived from partially fed females I. ricinus stimulate lymph node T cells from mice infested with I. ricinus larvae or nymphs. In contrast, lymph node cells from mice infested with Amblyomma hebraeum nymphs do not respond. A chromatographic fraction enriched with a 65 kDa protein (IrSG65) isolated from salivary glands of I. ricinus partially fed females induces in vitro a specific T cell proliferation of lymph node cells from mice infested with I. ricinus nymphs. The depletion of CD4+ T cells drastically reduces the ability of lymphocytes from infested mice to proliferate after IrSG65 stimulation.
Ganapamo
F
F
Department of Immunology, University of Neuchâtel, Switzerland.
Rutti
B
B
Brossard
M
M
D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002455:Cell Division; D002852:Chromatography, Ion Exchange; D005260:Female; D018884:Ixodes / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012469:Salivary Glands / Q000276:immunology; D013984:Tick Infestations / Q000276:immunology* / Q000469:parasitology
D000941:Antigens