Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs.
In eukaryotes, chromosomal DNA is licensed for a single round of replication in each cell cycle. Xenopus MCM3 protein has been implicated in the licensing of replication in egg extract. We have cloned cDNAs encoding five immunologically distinct proteins associated with Xenopus MCM3 as members of the MCM/P1 family. Six Xenopus MCM proteins formed a physical complex in the egg extract, bound to unreplicated chromatin before the formation of nuclei, and apparently displaced from replicated chromatin. The requirement of six XMCM proteins for the replication activity of the egg extract before nuclear formation suggests that their re-association with replicated chromatin at the end of the mitotic cell cycle is a key step for the licensing of replication.
Kubota
Y
Y
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Japan.
Mimura
S
S
Nishimoto
S
S
Masuda
T
T
Nojima
H
H
Takisawa
H
H
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002451:Cell Compartmentation; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002474:Cell-Free System; D002843:Chromatin / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004261:DNA Replication; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique; D064168:Minichromosome Maintenance Complex Component 7; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010063:Ovum; D010539:Permeability; D011233:Precipitin Tests; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014981:Xenopus; D029867:Xenopus Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D018797:Cell Cycle Proteins; D002843:Chromatin; D018076:DNA, Complementary; D009687:Nuclear Proteins; D029867:Xenopus Proteins; D000251:Adenosine Triphosphatases; C509836:Mcm7 protein, Xenopus; D064168:Minichromosome Maintenance Complex Component 7