title: [Flow cytometry for extensive thoracic diagnosis].
abstract: Immunophenotyping of cells by flow cytometry has become a routine test to diagnose pulmonary and mediastinal diseases. Peripheral blood, extravascular fluids, bronchoalveolar lavage (BAL) and suspension of single cells obtained by fine-needle aspiration can be used. Peripheral blood (MOAb for immunophenotyping of lymphocytes: CD14, CD45, CD3, CD19, CD4, CD8, CD16/56, HLA DR, CD38, CD25) is the material of choice for detection and monitoring of immunodeficiences. BAL (MOAb for immunophenotyping of lymphocytes: CD14, CD45, CD3, CD19, CD4, CD8, CD16/56, HLA DR) is used mainly for differential diagnosis of extrinsic allergic alveolitis (low CD4/CD8 ratio) and sarcoidosis (high CD4/CD8 ratio). The enumeration of alveolar macrophage subsets is an important tool to establish diagnosis of histiocytosis X (CD1a > 3%). Extravascular fluids, suspension of single cells and BAL are preferred materials for detection and classification of non-Hodgkin lymphomas (MOAb for immunophenotyping of lymphocytes: CD14, CD45, CD3, CD19, CD4, CD8, CD16/56, HLA DR, CD38, CD25, CD23, CD5, CDl1c, CD30, light chain immunoglobulins).
authors.affiliation: Pulmologischen Zentrums, Stadt Wien.
authors.forename: K
authors.initials: K
authors.lastname: Bernhardt
mesh_terms: D003937:Diagnosis, Differential; D005434:Flow Cytometry; D006689:Hodgkin Disease / Q000276:immunology / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D008168:Lung / Q000276:immunology / Q000473:pathology; D008171:Lung Diseases / Q000276:immunology / Q000473:pathology*; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000473:pathology; D008477:Mediastinal Diseases / Q000276:immunology / Q000473:pathology*; D008479:Mediastinal Neoplasms / Q000276:immunology / Q000473:pathology*; D008482:Mediastinum / Q000473:pathology