title: Protection of SIVmac-infected macaque monkeys against superinfection by a simian immunodeficiency virus expressing envelope glycoproteins of HIV type 1.
abstract: The infection of macaque monkeys by attenuated simian immunodeficiency virus can vaccinate against pathogenic molecular clones and isolates of the same virus. The correlates of this potent protective immunity are not fully understood but may be the key to an effective AIDS vaccine for humans. Aiming to determine whether host immune responses to envelope glycoprotein are an essential component of the immunity to primate lentiviruses, we have tried to superinfect SIVmac-infected macaque monkeys with SHIVsbg, a chimeric primate lentivirus constructed from the SIVmac239 genome with the env, rev, tat, and vpu genes from HIV-1 Lai. After inoculation of a large dose of SHIVsbg, the chimeric virus was isolated by coculture of mononuclear blood cells from four of five SIV-infected monkeys, but three animals were protected from extracellular SHIV viremia and did not seroconvert to HIV-1 glycoproteins. In the two SIV-infected monkeys that did develop SHIV viremia, cell-associated viral load was reduced at least 100-fold. These data indicate that an antiviral response capable of effectively controlling primate lentivirus replication might not necessarily involve the envelope glycoprotein.
authors.affiliation: INSERUM U74 et Laboratoire de Virologie, Strasbourg, France.
authors.forename: C S
authors.initials: CS
authors.lastname: Dunn
authors.forename: B
authors.initials: B
authors.lastname: Hurtrel
authors.forename: C
authors.initials: C
authors.lastname: Beyer
authors.forename: L
authors.initials: L
authors.lastname: Gloeckler
authors.forename: T N
authors.initials: TN
authors.lastname: Ledger
authors.forename: C
authors.initials: C
authors.lastname: Moog
authors.forename: M P
authors.initials: MP
authors.lastname: Kieny
authors.forename: M
authors.initials: M
authors.lastname: Mehtali
authors.forename: D
authors.initials: D
authors.lastname: Schmitt
authors.forename: J P
authors.initials: JP
authors.lastname: Gut
authors.forename: A
authors.initials: A
authors.lastname: Kirn
authors.forename: A M
authors.initials: AM
authors.lastname: Aubertin
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000097:blood; D015686:Gene Products, env / Q000276:immunology*; D005814:Genes, Viral; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000276:immunology* / Q000517:prevention & control; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D017958:Lentiviruses, Primate / Q000302:isolation & purification; D007963:Leukocytes, Mononuclear / Q000821:virology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D012367:RNA, Viral / Q000097:blood; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*; D015163:Superinfection / Q000276:immunology*; D019562:Viral Load
chemical_list: D016915:AIDS Vaccines; D000914:Antibodies, Viral; D004279:DNA, Viral; D015686:Gene Products, env; D015483:HIV Antibodies; D012367:RNA, Viral