Effects of pyruvate-based or lactate-based peritoneal dialysis solutions on neutrophil intracellular pH.
Acidic (pH 5.2) incubation mixtures containing pyruvate-based or lactate-based peritoneal dialysis solutions (PDSN's) induced comparable degrees of intracellular acidosis in neutrophils. However, addition of an acidic (pH 5.2), pyruvate-based PDSN to a pH-7.4, neutrophil/phosphate-buffered saline mixture brought about higher extracellular and intracellular (neutrophil) pH values when compared to the introduction of an equally acidic, lactate-based PDSN. This poor ability of acidic (pH 5.0-5.5), pyruvate-based PDSN's to resist alkalinizing influences is the cause for the above higher pH values. The higher intracellular pH levels so obtained may be a reason behind why acidic, pyruvate-based PDSN's appear to be more biocompatible than their equally acidic, lactate-based counterparts.
Ing
T S
TS
Veterans Affairs Hospital, Hines, Illinois, USA.
Zhou
X J
XJ
Yu
A W
AW
Zhou
F Q
FQ
Vaziri
N D
ND
D000328:Adult; D001672:Biocompatible Materials; D015314:Dialysis Solutions / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D019344:Lactic Acid; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D010530:Peritoneal Dialysis; D019289:Pyruvic Acid
D001672:Biocompatible Materials; D015314:Dialysis Solutions; D019344:Lactic Acid; D019289:Pyruvic Acid