Chemical synthesis of a 7 kDa insect gonadotropic neurohormone.
An original insect neurohormone of 65 residues was synthesized by the solid-phase methodology using t-Boc strategy and Boc-Val-PAM-resin. The purification, conducted by several steps of liquid chromatography having mass, polarity or charge as separative criteria, yielded the product with the correct molecular weight of 6922 Da determined by mass spectrometry. The synthetic peptide had both the same affinity for the anti-native neurohormone serum and the same biological activity as the native neurohormone.
Girardie
J
J
Laboratoire de Neuroendocrinologie, Université Bordeaux 1, Talence, France.
Girardie
A
A
Van Dorsselaer
A
A
Sorokine
O
O
Geoffre
S
S
Hospital
M
M
Precigoux
G
G
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006062:Gonadotropins / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D006110:Grasshoppers; D007120:Immunochemistry; D007301:Insect Hormones / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D019476:Insect Proteins / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D010053:Ovary / Q000187:drug effects / Q000254:growth & development
D006062:Gonadotropins; D007301:Insect Hormones; D019476:Insect Proteins; D009419:Nerve Tissue Proteins; C072215:ovary maturating parsin protein, Locusta migratoria