Cloning and nucleotide sequence of Bacillus stearothermophilus pyruvate carboxylase.
A gene for prokaryotic pyruvate carboxylase (PC) was cloned from Bacillus stearothermophilus. It has an open reading frame of 3441 base pairs which can code for a protein of 128,353 Da. Not only the molecular size and domain organization but also the deduced amino acid sequence of B. stearothermophilus PC are similar to those of eukaryotic PCs.
Kondo
H
H
Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Japan. kondo@bse.kyutech.ac.jp
Kazuta
Y
Y
Saito
A
A
Fuji
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D001411:Geobacillus stearothermophilus / Q000145:classification / Q000201:enzymology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011766:Pyruvate Carboxylase / Q000235:genetics*
D004269:DNA, Bacterial; D011766:Pyruvate Carboxylase