Inducible expression of the beta 1 subunit of the sodium pump.
The Na,K-ATPase, or sodium pump, a ubiquitous transmembrane enzyme in higher eukaryotes, consists of an alpha and a beta subunit. Here we investigate the expression pattern of the two beta isotypes in mouse B cell lines. Neither primary cells nor cell lines express beta 2. Abelson virus-transformed pre-B cells express beta 1, while B lymphomas and plasmacytomas do not. Thus, beta 1 expression in transformed cells follows that of their untransformed counterparts. Some subclones of pre-B cell line 70Z/3 express beta 1, and others do not, but lipopolysaccharide induces the beta 1-negative cells to become beta 1-positive.
Masat
L
L
Department of Microbiology and Immunology, University of California, San Francisco 94143-0670, USA.
Wabl
M
M
D000011:Abelson murine leukemia virus; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D001921:Brain / Q000166:cytology; D002460:Cell Line; D002472:Cell Transformation, Viral; D004790:Enzyme Induction; D005786:Gene Expression Regulation; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000201:enzymology / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010954:Plasmacytoma / Q000201:enzymology / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis* / Q000235:genetics
D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase