Enhanced renal susceptibility to ischemia-reperfusion injury in the rat with obstructive jaundice.
OBJECTIVE
To investigate the susceptibility of kidneys to ischemia-reperfusion injury under obstructive jaundice.


METHODS
Bile ducts of male rats were ligated for five days, right kidneys were removed, and vascular clamps were placed across left renal arteries for 30 minutes.


RESULTS
Twenty-four hours later, ischemia-reperfusion produced renal injury in jaundiced rats as shown by increased serum creatinine and urea nitrogen levels. Histological observation showed tubular damages. These changes were minimal after ischemia-reperfusion in control rats. Renal thiobarbituric acid reactive substance contents were increased after bile duct ligation, which did not change after ischemia-reperfusion. On the other hand, ischemia-reperfusion produced a significant increase in renal thiobarbituric acid reactive substance contents in control rats. Renal glutathione contents were increased two fold after bile duct ligation and they were significantly decreased by ischemia-reperfusion.


CONCLUSIONS
Kidneys in obstructive jaundice appear to be susceptible to ischemia-reperfusion injury. Although protective roles of GSH is suggested, the role of oxygen radicals in this type of renal injury remains to be further elucidated.
Tajiri
K
K
Second Department of Internal Medicine, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Miyakawa
H
H
Liu
J
J
Kamiyama
T
T
Marumo
F
F
Sato
C
C
D000818:Animals; D001806:Blood Urea Nitrogen; D002779:Cholestasis / Q000150:complications*; D003404:Creatinine / Q000097:blood; D004198:Disease Susceptibility; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051437:Renal Insufficiency / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D015427:Reperfusion Injury / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D003404:Creatinine; D005978:Glutathione