title: Isolation of viable egg cells of rape (Brassica napus L.).
abstract: The isolation of viable egg cells of rape (Brassica napus L.) has been achieved from microdissected ovules. The non-gametic cells of the embryo sac, synergids and central cells have also been isolated. Their morphology corresponded to that of these cells in situ, making a discrimination from isolated sporophytic cells possible. Two hours after isolation the egg cells were still viable. Viable egg cells have been reproducibly isolated with a frequency of 25% per dissected ovule.
authors.affiliation: Japan Tobacco Inc., Oyama, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Katoh
authors.forename: H
authors.initials: H
authors.lastname: Lörz
authors.forename: E
authors.initials: E
authors.lastname: Kranz
mesh_terms: D001937:Brassica / Q000166:cytology*; D002469:Cell Separation; D008856:Microscopy, Fluorescence; D010063:Ovum / Q000166:cytology*; D013194:Staining and Labeling