Cytokine mediated growth inhibition of HTLV-I infected transformed human lymphocytes.
The interaction between HTLV-I infected immortalized cells and transformed cells was studied. HTLV-I infected immortalized human lymphocytes (Coculture-5) dependent of IL-2 medium (IL-2+PHA+TPA) and transformed cells (UV-5) derived from Coculture-5 following UV irradiation were cocultured in equal numbers in IL-2 medium. UV-5 cells were suppressed and disappeared about 1 month in cocultivation. UV-5 cells were not suppressed in IL-2 medium. Coculture-5 cells and UV-5 cells were seeded in Transwell dishes that were separated by membrane with pores 0.4 micron in diameter and cultured in IL-2 medium. UV-5 cells were suppressed indicating that cell-cell contact was not required for the observed suppression. UV-5 cells were markedly suppressed in IL-2 medium harvested from Coculture-5 cells when recombinant interferon alpha (1000 U/ml) was added to the medium. These findings demonstrated that growth of HTLV-I infected transformed cells could be suppressed by cytokine(s) released by HTLV-I carrier lymphocytes.
Mochizuki
S
S
Div. Pathol., Chiba Center Research Institute, Japan.
Kawamura
K
K
Koshikawa
N
N
Maruyama
K
K
D002455:Cell Division / Q000187:drug effects* / Q000528:radiation effects; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002871:Chromosome Banding; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D007621:Karyotyping; D011994:Recombinant Proteins; D013601:T-Lymphocytes; D014466:Ultraviolet Rays
D017077:Culture Media, Conditioned; D007370:Interferon Type I; D007376:Interleukin-2; D011994:Recombinant Proteins