Solid-phase synthesis and cellular localization of a C- and/or N-terminal labelled peptide.
We report the solid-phase synthesis by the Fmoc strategy of a peptide containing a cysteamide group at its C-terminus. This peptide was subjected to further modifications including the linkage of fluorophores, namely lucifer yellow and coumarin respectively, at the C- and/or N-terminals. After incubation with living cultured cells these two probes were localized and it is concluded that the post-synthesis modifications can strongly modify the localization of the peptide.
Vidal
P
P
Centre de Recherche de Biochimie Macromoléculaire, Montpellier, France.
Chaloin
L
L
Méry
J
J
Lamb
N
N
Lautredou
N
N
Bennes
R
R
Heitz
F
F
D000595:Amino Acid Sequence; D005453:Fluorescence; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis* / Q000138:chemical synthesis*; D013057:Spectrum Analysis
D010455:Peptides