[A dynamic study of the cytotoxic effects of hyperthermia combined with cis-diaminedichlorolplatinum (DDP) on human gastric cancer cell lines MKN28 and MKN45 in vitro].
Cytotoxic effects of hyperthermia combined with DDP on MKN28 and MKN45 cells were studied by MTT assay according to a nested design. The rusults showed: hyperthermia alone above 43 degrees C for 30 min was cytotoxic; hyperthermia at temperature lower than 43 degrees C for 30 min could increase sensitivity of cancer cells to DDP. The cytotoxic effect of simultaneous use of hyperthermia and DDP was more marked than that of sequential use of the 2 treatments. Hyperthermia combined with DDP could inhibit growth of human gastric adenocarcinoma cells regardless of their degree of differentiation.
Chen
W
W
Department of Gastroenterology, First Affiliated Hospital, Hang Zhou.
Ji
F
F
Huang
H
H
D000230:Adenocarcinoma / Q000473:pathology*; D002945:Cisplatin / Q000494:pharmacology*; D006801:Humans; D006979:Hyperthermia, Induced; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002945:Cisplatin