Separation of extracts from biological tissues into polycyclic aromatic hydrocarbon, polychlorinated biphenyl and polychlorinated dibenzo-p-dioxin/polychlorinated dibenzofuran fractions prior to analysis.
A low-pressure liquid chromatography method is presented for separating polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/polychlorinated dibenzofurans (PCDDs/PCDFs) from biological tissue extracts. After removing lipid from extracts, the PAHs are separated from PCBs and PCDDs/PCDFs on a deactivated 13-24 microns silica gel column. The PCBs are subsequently separated from PCDDs/PCDFs by collecting the first fraction from an automated three column cleanup procedure for PCDDs/PCDFs. The complete method has been used to obtain high recoveries of the three compound classes for analysis by GC-electron capture detection (PCBs) or GC-MS (PAHs and PCDDs/PCDFs).
O'Keefe
P W
PW
School of Public Health, State University of New York at Albany, USA.
Miller
J
J
Smith
R
R
Connor
S
S
Clayton
W
W
Storm
R
R
D000818:Animals; D001572:Benzofurans / Q000032:analysis*; D002683:Chironomidae; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D003199:Computer Systems; D000072338:Dibenzofurans, Polychlorinated; D007814:Larva; D010316:Particle Size; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis; D011083:Polycyclic Compounds / Q000032:analysis*; D012474:Salmon; D012822:Silicon Dioxide
D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins; D011083:Polycyclic Compounds; D012822:Silicon Dioxide; D011078:Polychlorinated Biphenyls