Identification of differentially expressed mRNAs during rat C6 glial cell differentiation by mRNA fingerprinting using arbitrarily primed PCR (RAP).
Differentiation of glial cells is controlled by a complex program of differential expressions of many genes. To identify differentially expressed genes that are involved in rat C6 glial cell differentiation induced by dibutyryl cyclic AMP and theophylline, mRNA fingerprinting using arbitrarily primed PCR (RAP) was used. Four cDNA fragments, that were differentially expressed during differentiation, were isolated. Sequence analysis revealed that one of them, abundantly expressed during differentiation, was homologous to a hamster calcium-dependent serine protease. Another one was highly similar to rabbit dystrobrevin and the other two clones were identical to rat triose phosphate isomerase and calnexin. The results obtained suggest that the expressions of particular genes were changed and that RAP is a useful method to identify genes which are differentially expressed during glial cell differentiation.
Sakai
H
H
Department of Neurosurgery, Gifu University School of Medicine, Japan. nohgeka@cc.gifu-u.ac.jp
Nakashima
S
S
Yoshimura
S
S
Nishimura
Y
Y
Sakai
N
N
Nozawa
Y
Y
D000818:Animals; D001345:Autoradiography; D002454:Cell Differentiation / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D009457:Neuroglia / Q000378:metabolism*; D009710:Nucleotide Mapping; D016133:Polymerase Chain Reaction; D051381:Rats