Lack of interleukin 10 expression in monocyte-derived macrophages in response to in vitro infection by HIV type 1 isolates.
Interleukin 10 is a pleiotropic cytokine that is overexpressed in HIV-infected patients. Here, we investigated IL-10 expression in primary cultures of monocyte-derived macrophages (MDMs) in response to in vitro infection by HIV-1/Ba-L or two macrophage-tropic HIV-1 primary isolates. Whatever the multiplicity of infection used, and in spite of high replication levels and an increase in HIV-infected cell frequency, neither significant IL-10 secretion nor IL-10 mRNA overexpression was induced in HIV-1-infected MDMs. Moreover, identical results were obtained with HIV-1-infected 1-day monocytes. These results show that MDM infection by HIV is not sufficient by itself for inducing IL-10 synthesis.
Dereuddre-Bosquet
N
N
Département de Recherche Médicale, Institut Paris-Sud sur les Cytokines, Centre de Recherches du Service de Santé des Armées, Fontenay aux Roses, France.
Clayette
P
P
Martin
M
M
Benveniste
O
O
Fretier
P
P
Jaccard
P
P
Vaslin
B
B
Lebeaut
A
A
Dormont
D
D
D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000276:immunology; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D019562:Viral Load; D014779:Virus Replication
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D054303:HIV Reverse Transcriptase