Effects of oviductal fluid and heparin on fertility and characteristics of porcine spermatozoa.
The objective of this study was to determine the effects of oviductal fluid and heparin on sperm penetration and the characteristics of spermatozoa. The addition of oviductal fluid and heparin to the fertilisation medium decreased sperm penetration and the mean number of spermatozoa in penetrated eggs. The number of spermatozoa firmly bound to zona pellucida was also decreased in the presence of oviductal fluid and heparin. Chlortetracycline (CTC) fluorescence patterns were used to determine the incidence of capacitation and the acrosome reaction. The proportion of capacitated and acrosome-free spermatozoa increased when spermatozoa were exposed for 1.5 and 3 h to oviductal fluid and heparin. In contrast heparin alone did not increase the number of capacitated spermatozoa at these time points. These results suggest that factor(s) in oviductal secretions reduce polyspermic fertilisation and the number of spermatozoa that will penetrate porcine oocytes. The reduction of polyspermic penetration by oviductal secretions may be due to a reduced number of spermatozoa in the fertilisation medium with an intact acrosome.
Kim
N H
NH
Department of Animal Science, Kon Kuk University, Seoul, Korea.
Day
B N
BN
Lim
J G
JG
Lee
H T
HT
Chung
K S
KS
D000818:Animals; D001826:Body Fluids / Q000502:physiology; D005187:Fallopian Tubes / Q000502:physiology*; D005260:Female; D005298:Fertility / Q000187:drug effects; D005307:Fertilization in Vitro; D006493:Heparin / Q000494:pharmacology*; D008297:Male; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013552:Swine
D006493:Heparin