Prostaglandin levels in BL6 melanoma cells cultured in vitro: the effect of vitamin E succinate supplementation.
Malignant murine melanoma (BL6-F10) cells convert arachidonic acid primarily to PGD2, PGF2alpha, PGE2, PGI2 in descending order of magnitude. Supplementation with 1-10 microg/ml vitamin E succinate resulted in a significant (P < or = 0.05) decrease in PGD2 levels at vitamin concentrations of 3, 5, 7 and 10 microg/ml respectively, while PGF2alpha levels were significantly decreased at 1, 3, 5 (P < or = 0.05), 7 and 10 microg/ml (P < or = 0.01) vitamin E succinate. BL6-F10 cells supplemented with 7 and 10 microg/ml vitamin E succinate showed a marked increase in PGE2 levels with a significant increase occurring at 10 microg/ml (P < or = 0.025). PGI2 levels followed a similar trend to PGE2 with a significant increase (P < or = 0.05) occurring at 10 microg/ml.
Ottino
P
P
Department of Biochemistry and Microbiology, Rhodes University, J Grahamstown, South Africa.
Duncan
J R
JR
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D011464:Epoprostenol / Q000378:metabolism; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D015230:Prostaglandin D2 / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism*; D024505:Tocopherols; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
D011453:Prostaglandins; D014810:Vitamin E; D016718:Arachidonic Acid; D015237:Dinoprost; D011464:Epoprostenol; D015232:Dinoprostone; D024505:Tocopherols; D015230:Prostaglandin D2