title: Comparative effects of bovine cytokines on cattle and bison peripheral blood mononuclear cell proliferation.
abstract: Proliferation of peripheral blood mononuclear cells (PBMC) from cattle and bison was measured following stimulation of PBMC with bovine cytokines. Bovine interleukin 1 beta (BoIL-1 beta), interleukin 2 (BoIL-2) or granulocyte-macrophage colony-stimulating factor (BoGM-CSF) at 0.1-100 U/ml were incubated for 48 h with PBMC alone or with PBMC and various mitogens. These included concanavalin A (Con A), phytohemagglutinin (PHA), pokeweed mitogen (PWM) or Escherichia coli 055:B5 lipopolysaccharide (LPS) at 10-0.1 micrograms/ml. BoIL-2 alone, but not BoIL-1 beta and BoGM-CSF alone, induced proliferation of cattle and bison PBMC in the absence of mitogens. In addition, BoIL-1 beta and BoIL-2, but not BoGM-CSF, enhanced proliferation of cattle and bison PBMC induced by mitogens. These results indicate that BoIL-1 beta and BoIL-2 stimulate cattle and bison PBMC proliferation in a similar manner, whereas BoGM-CSF does not appear capable of stimulating either cattle or bison PBMC proliferation.
authors.affiliation: National Animal Disease Center, USDA, Ames, Iowa, 50010, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Stevens
authors.forename: S C
authors.initials: SC
authors.lastname: Olsen
authors.forename: N F
authors.initials: NF
authors.lastname: Cheville
mesh_terms: D000818:Animals; D016164:Bison / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D016207:Cytokines / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000302:isolation & purification / Q000494:pharmacology; D007375:Interleukin-1 / Q000302:isolation & purification / Q000494:pharmacology; D007376:Interleukin-2 / Q000302:isolation & purification / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D013045:Species Specificity
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D007376:Interleukin-2; D008934:Mitogens; D016178:Granulocyte-Macrophage Colony-Stimulating Factor