title: Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies. Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the alpha5 subunit.
abstract: The high affinity interaction of integrin alpha5beta1 with the central cell binding domain (CCBD) of fibronectin requires both the Arg-Gly-Asp (RGD) sequence (in the 10th type III repeat) and a second site (in the adjacent 9th type III repeat) which synergizes with RGD. We have attempted to map the fibronectin binding interface on alpha5beta1 using monoclonal antibodies (mAbs) that inhibit ligand recognition. The binding of two anti-alpha5 mAbs (P1D6 and JBS5) to alpha5beta1 was strongly inhibited by a tryptic CCBD fragment of fibronectin (containing both synergy sequence and RGD) but not by GRGDS peptide. Using recombinant wild type and mutated fragments of the CCBD, we show that the synergy region of the 9th type III repeat is involved in blocking the binding of P1D6 and JBS5 to alpha5beta1. In contrast, binding of the anti-beta1 mAb P4C10 to alpha5beta1 was inhibited to a similar extent by GRGDS peptide, the tryptic CCBD fragment, or recombinant proteins lacking the synergy region, indicating that the RGD sequence is involved in blocking P4C10 binding. P1D6 inhibited the interaction of a wild type CCBD fragment with alpha5beta1 but had no effect on the binding of a mutant fragment that lacked the synergy region. The epitopes of P1D6 and JBS5 mapped to the NH2-terminal repeats of the alpha5 subunit. Our results indicate that the synergy region is recognized primarily by the alpha5 subunit (in particular by its NH2-terminal repeats) but that the beta1 subunit plays the major role in binding of the RGD sequence. These findings provide new insights into the mechanisms, specificity, and topology of integrin-ligand interactions.
authors.affiliation: Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom. pmould@fs1.scq.man.ac.uk
authors.forename: A P
authors.initials: AP
authors.lastname: Mould
authors.forename: J A
authors.initials: JA
authors.lastname: Askari
authors.forename: S i
authors.initials: Si
authors.lastname: Aota
authors.forename: K M
authors.initials: KM
authors.lastname: Yamada
authors.forename: A
authors.initials: A
authors.lastname: Irie
authors.forename: Y
authors.initials: Y
authors.lastname: Takada
authors.forename: H J
authors.initials: HJ
authors.lastname: Mardon
authors.forename: M J
authors.initials: MJ
authors.lastname: Humphries
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D001665:Binding Sites; D018604:Epitope Mapping; D006801:Humans; D039482:Integrin alpha5; D019012:Integrin beta1 / Q000276:immunology*; D009842:Oligopeptides / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D017469:Receptors, Fibronectin / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039482:Integrin alpha5; D019012:Integrin beta1; D009842:Oligopeptides; D010446:Peptide Fragments; D017469:Receptors, Fibronectin; C047981:arginyl-glycyl-aspartic acid; D014357:Trypsin