Mycothiol, 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D- myo-inositol, is the factor of NAD/factor-dependent formaldehyde dehydrogenase.
Two different NAD/coenzyme-dependent formaldehyde dehydrogenases exist, the well-known NAD/GSH-dependent (EC 1.2.1.1) and the more recently discovered NAD/Factor-dependent enzyme. The GSH-dependent one has been found in eukaryotes and Gram-negative bacteria, the Factor-dependent one in two different Gram-positive bacteria. Previous work also showed that Factor and GSH are not interchangeable in the enzymatic reactions. Here it is revealed that the Factor is identical to mycothiol (MySH), 1-O-(2'-[N-acetyl-L-cysteinyl]-amido-2'-deoxy-alpha-D-glucopyranosyl)-D- myo-inositol, a thiol compound which has recently been detected in Actinomycetes. Thus, MySH is GSH's companion as it is the coenzyme for the enzyme which henceforth can be indicated as NAD/MySH-dependent formaldehyde dehydrogenase.
Misset-Smits
M
M
Department of Microbiology and Enzymology, Delft University of Technology, The Netherlands.
van Ophem
P W
PW
Sakuda
S
S
Duine
J A
JA
D000445:Aldehyde Oxidoreductases / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine; D004187:Disaccharides / Q000737:chemistry*; D006020:Glycopeptides; D007294:Inositol; D009243:NAD / Q000502:physiology*; D011720:Pyrazoles; D013302:Streptomyces / Q000201:enzymology; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D001426:Bacterial Proteins; D004187:Disaccharides; D006020:Glycopeptides; D011720:Pyrazoles; D013438:Sulfhydryl Compounds; C089265:mycothiol; D009243:NAD; D007294:Inositol; C020145:formaldehyde dehydrogenase (glutathione); D000445:Aldehyde Oxidoreductases; D003545:Cysteine