title: Cloning of a gene (RIG-G) associated with retinoic acid-induced differentiation of acute promyelocytic leukemia cells and representing a new member of a family of interferon-stimulated genes.
abstract: In a cell line (NB4) derived from a patient with acute promyelocytic leukemia, all-trans-retinoic acid (ATRA) and interferon (IFN) induce the expression of a novel gene we call RIG-G (for retinoic acid-induced gene G). This gene codes for a 58-kDa protein containing 490 amino acids with several potential sites for post-translational modification. In untreated NB4 cells, the expression of RIG-G is undetectable. ATRA treatment induces the transcriptional expression of RIG-G relatively late (12-24 hr) in a protein synthesis-dependent manner, whereas IFN-alpha induces its expression early (30 min to 3 hr). Database search has revealed a high-level homology between RIG-G and several IFN-stimulated genes in human (ISG54K, ISG56K, and IFN-inducible and retinoic acid-inducible 58K gene) and some other species, defining a well conserved gene family. The gene is composed of two exons and has been mapped by fluorescence in situ hybridization to chromosome 10q24, where two other human IFN-stimulated gene members are localized. A synergistic induction of RIG-G expression in NB4 cells by combined treatment with ATRA and IFNs suggests that a collaboration exists between their respective signaling pathways.
authors.affiliation: Key Laboratory of Genome Research, Shanghai Institute of Hematology, Rui Jin Hospital, Shanghai Second Medical University, Shanghai, 200025, People's Republic of China.
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: J H
authors.initials: JH
authors.lastname: Tong
authors.forename: M
authors.initials: M
authors.lastname: Mao
authors.forename: L X
authors.initials: LX
authors.lastname: Kan
authors.forename: M M
authors.initials: MM
authors.lastname: Liu
authors.forename: Y W
authors.initials: YW
authors.lastname: Sun
authors.forename: G
authors.initials: G
authors.lastname: Fu
authors.forename: Y K
authors.initials: YK
authors.lastname: Jing
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: D
authors.initials: D
authors.lastname: Lepaslier
authors.forename: M
authors.initials: M
authors.lastname: Lanotte
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Waxman
authors.forename: Y X
authors.initials: YX
authors.lastname: Wang
authors.forename: J Z
authors.initials: JZ
authors.lastname: Tan
authors.forename: S J
authors.initials: SJ
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D016147:Genes, Tumor Suppressor; D006801:Humans; D007372:Interferons / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; C116468:IFIT3 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D014212:Tretinoin; D007372:Interferons