title: [Microsurgical pancreatoduodenal transplantation in rats. Technique and results following 12 years of investigation].
abstract: In this study we present the technical details, adaptations and modifications of the original procedure of pancreaticoduodenal transplantation in rats described by Lee et al. in 1972. We also present the results and technical failures observed in a follow-up of 12 years. From March, 1982 to December, 1994, we performed in the Laboratory of Surgical Technique and Experimental Surgery of Faculty of Medicine, Botucatu-UNESP, Brazil, 665 duodenopancreatectomies in donor rats and 592 surgeries for revascularization of the pancreatic graft in recipient animals. The observed percentage of technical failures in donor rats was 11% due to bleeding and/or vascular complications, irregular flushing of the graft with saline and respiratory insufficiency. In recipients of grafts, we observed a percentage of technical failures of 22.5% due to porto-caval thrombosis, vascular bleeding, pancreatitis and graft ischemia. In both surgeries, the successful results are directly related to the technical performance of the surgeon and the cares in the postoperative period.
authors.affiliation: Departamento de Cirurgia e Ortopedia, FMB-UNESP.
authors.forename: C T
authors.initials: CT
authors.lastname: Spadella
authors.forename: M C
authors.initials: MC
authors.lastname: Mercadante
authors.forename: J L
authors.initials: JL
authors.lastname: Machado
authors.forename: S A
authors.initials: SA
authors.lastname: Schellini
authors.forename: A R
authors.initials: AR
authors.lastname: de Macedo
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D004386:Duodenum / Q000637:transplantation*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008866:Microsurgery / Q000379:methods; D016035:Pancreas Transplantation / Q000379:methods*; D016577:Pancreaticoduodenectomy; D011183:Postoperative Complications; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D017211:Treatment Failure