[Carcinogenic mechanisms of multiple genes in cervical carcinoma].
The alterations of multiple genes and their carcinogenic mechanism in cervical carcinoma were studies by molecular hybridisation, PCR and PCR-ASO techniques. The G-T point mutation in the 12th coden of Ha-ras was detected in cervical carcinomas with mutation frequency of 18.2% (8/44), and the amplification rate of Ha-ras gene was 45% (9/20). The c-erb B2 was amplified 3-30 fold with an amplification rate of 73.3% (11/15) in cervical carcinomas and 5 cancerous samples showed gene rearrangement. The elevated copies of c-myc gene with amplification rate of 91.7% (11/12) were observed in cervical carcinomas. The study of HPV16 viral gene showed that the existence of HPV16 DNA sequence was positively associated with c-myc gene amplification in cervical cancerous samples. The p53 and Rb tumor suppressor genes absence of deletion were observed in the 12 specimens of cervical carcinoma investigated. As mentioned above, the study on alteration and carcinogenic mechanism of multiple genes indicated that 3 oncogenes and HPV16 viral gene were activated or integrated throygh different mechanisms and they played roles in co-carcinogenesis. The integration of HPV16 gene might promote the c-myc gene at the early stage in carcinogenesis of cervical carcinoma, while the alteration of Ha-ras and c-erb B2 gene might be middle-late event. As for the roles of the p53 and Rb tumor suppresor gene in cervical carcinogenesis need further researches.
Huang
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G
Institute of Cancer Research, Chengdu.
Xiao
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X
Huang
Y
Y
Huang
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R
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D015321:Gene Rearrangement / Q000235:genetics; D005814:Genes, Viral; D018734:Genes, erbB-2 / Q000235:genetics; D016259:Genes, myc / Q000235:genetics; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D017354:Point Mutation; D011519:Proto-Oncogenes / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*