title: Immunoperoxidase for the detection of antibodies in cerebrospinal fluid in neurocysticercosis: use of Cysticercus cellulosae and Cysticercus longicollis particles fixed on microscopy slides.
abstract: The ORF strain of Cysticercus longicollis represents an important model for the study of heterologous antigens in the immunodiagnosis of neurocysticcreosis (NC). The immunoperoxidase (IP) technique was standardized using a particulate antigen suspension of Cysticercus longicollis (Cl) and Cysitcercus cellulosae (Cc). Cerebrospinal fluid (CSF) samples were incubated on the antigen fixed to microscopy slides; the conjugate employed was anti-IgG-peroxidase and the enzymatic reaction was started by covering the slides with chromogen solution (diaminobenzidine/H2O2). After washing with distilled water, the slide was stained with 2% malachite green in water. Of the CSF samples from 21 patients with NC, 19 (90.5%) were positive, whereas the 8 CSF samples from the control group (100%) were negative. The results of the [P-C] test applied to 127 CSF samples from patients with suspected NC showed 28.3% reactivity as opposed to 29.1% for the IP-Cc test. The agreement index for the IP test (Cl x Cc) was 94.2%, with no significant difference between the two antigens.
authors.affiliation: Faculdade de Ciências Farmacèuticas, Universidade de São Paulo, Brasil.
authors.forename: A P
authors.initials: AP
authors.lastname: de Andrade
authors.forename: A J
authors.initials: AJ
authors.lastname: Vaz
authors.forename: P M
authors.initials: PM
authors.lastname: Nakamura
authors.forename: V S
authors.initials: VS
authors.lastname: Palou
authors.forename: R A
authors.initials: RA
authors.lastname: da Cunha
authors.forename: A W
authors.initials: AW
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000134:cerebrospinal fluid* / Q000302:isolation & purification*; D003551:Cysticercosis / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000276:immunology*; D003552:Cysticercus / Q000276:immunology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth