Increased expression and secretion of ICAM-1 during experimental infection with Trypanosoma cruzi.
In the present study we demonstrate that spleens and hearts from BALB/c mice infected with the virulent Tulahuén or the low virulent CA-I strains of Trypanosoma cruzi, contain substantially higher ICAM-1 transcripts than uninfected controls. ICAM-1 expression in heart cells was also increased in the protein level, as measured by flow cytometry, ELISA and immunohistochemistry. The adhesive receptor was observed not only on inflammatory cells but also on sarcolemma of cardiac myocytes from T. cruzi infected mice. ICAM-1 expression was higher during the acute phase than in the chronic phase of infection, and paralleled the density of inflammatory leukocytes. Elevated titres of soluble ICAM-1 (s-ICAM-1) were detected in sera from mice during the acute phase of infection with CA-I or Tulahuén parasites. Cytokines, including IFN-gamma, IL-1 alpha, IL-6 and TNF-alpha have been shown to modulate expression of ICAM-1. Spleens and hearts from mice infected with CA-I or Tulahuen strains showed increased accumulation of mRNAs specific for these cytokines, which peaked during the acute phase of infection. However, IFN-gamma activity was not necessary for ICAM-1 induction, as its levels were also increased during infection in IFN-gamma receptor knock-out (IFN-gamma R- ) mice. Upregulation of ICAM-1 expression might be a direct consequence of parasite infection, since its density on cell lines of different lineages was enhanced after 24 or 48 h of infection with T. cruzi.
Laucella
S
S
Instituto Nacional de Chagas Dr M. Fatala Chaben, Buenos Aires, Agentina.
Salcedo
R
R
Castaños-Velez
E
E
Riarte
A
A
De Titto
E H
EH
Patarroyo
M
M
Orn
A
A
Rottenberg
M E
ME
D000818:Animals; D001483:Base Sequence; D014355:Chagas Disease / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics*; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009206:Myocardium / Q000276:immunology; D016133:Polymerase Chain Reaction; D017471:Receptors, Interferon / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000472:pathogenicity; D015854:Up-Regulation; D014774:Virulence; D000096743:Interferon gamma Receptor
D016207:Cytokines; D017931:DNA Primers; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor