title: Modification of DNA duplexes to smooth their thermal stability independently of their base content for DNA sequencing by hybridization.
abstract: The possibility of equalizing DNA duplex stability is essential for the application of sequencing by hybridization. In this paper we describe a new strategy to obtain DNA duplexes with a thermal stability independent of their base content. Modified *C bases have been developed and incorporated into oligonucleotides. The influence of these modifications on duplex stability has been studied by absorption spectroscopy, thus allowing selection of N -4-ethyl-2'-deoxycytidine (d4EtC), which hybridizes specifically with natural dG to give a G4EtC base pair whose stability is very close to that of natural AT base pairs. Duplexes built with AT and/or G4EtC base pairs exhibit thermal stabilities independent of their base content in a classical buffer solution, thus enabling control of the stability of DNA hybrids as a function of their length only.
authors.affiliation: Centre de Biophysique Moléculaire, CNRS, rue Charles Sadron, 45071 Orléans Cedex 02, France and 1 Appligène-Oncor, Parc d'Innovation, BP 72, 67407 Illkirch, France.
authors.forename: H K
authors.initials: HK
authors.lastname: Nguyen
authors.forename: P
authors.initials: P
authors.lastname: Auffray
authors.forename: U
authors.initials: U
authors.lastname: Asseline
authors.forename: D
authors.initials: D
authors.lastname: Dupret
authors.forename: N T
authors.initials: NT
authors.lastname: Thuong
mesh_terms: D001482:Base Composition; D003562:Cytidine; D004247:DNA / Q000737:chemistry*; D003841:Deoxycytidine / Q000737:chemistry; D006151:Guanosine; D006361:Heating; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D017422:Sequence Analysis, DNA
chemical_list: D009838:Oligodeoxyribonucleotides; D003841:Deoxycytidine; D006151:Guanosine; D003562:Cytidine; D004247:DNA