Molecular cloning and sequencing of the cDNA encoding the catalytic subunit of mouse glutamate-cysteine ligase.
Reverse transcription-polymerase chain reaction (RT-PCR) was used for the enzymatic synthesis of cDNA sequences encompassing the open reading frame for the catalytic subunit of mouse kidney glutamate-cysteine ligase (Glclc). Comparison of the mouse Glclc cDNA sequence and predicted protein sequence with that of rat Glclc and human GLCLC revealed between 94.8% and 88.4% cDNA homology and 98.4% to 95% amino acid identity, respectively.
Reid
L L
LL
Department of Environmental Health, University of Washington, Seattle, USA.
Botta
D
D
Lu
Y
Y
Gallagher
E P
EP
Kavanagh
T J
TJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D005721:Glutamate-Cysteine Ligase / Q000235:genetics*; D007668:Kidney / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D005721:Glutamate-Cysteine Ligase