Molecular handling of photosynthetic proteins for molecular assembly construction.
Methods of constructing proteins were examined with special reference to the molecular assembly using photosynthetic RCs as membrane proteins. Molecular assemblies at the interfaces were studied by LB films, adsorption to the surface and reconstitution into liposomes and bilayer lipid membranes. The applications of biological specific ligands (recognition and binding), combinatorial chemical method, 2-D and 3-D order array assemblies and modification of protein molecules to make fusion proteins, as well as physical methods of manipulation of molecules by AFM tips and electric fields were reviewed.
Miyake
J
J
National Institute for Advanced Interdisciplinary Research, AIST/MITI, Ibaraki, Japan.
Hara
M
M
D000327:Adsorption; D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000648:ultrastructure; D019617:Halobacterium salinarum / Q000378:metabolism; D008051:Lipid Bilayers; D008961:Models, Structural; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000648:ultrastructure; D011107:Polylysine; D012242:Rhodobacter sphaeroides / Q000378:metabolism; D012241:Rhodopseudomonas / Q000378:metabolism
D008051:Lipid Bilayers; D045322:Photosynthetic Reaction Center Complex Proteins; D011107:Polylysine; D001436:Bacteriorhodopsins