title: Sequences of terminal non-coding regions from four dengue-2 viruses isolated from patients exhibiting different disease severities.
abstract: We have determined the 5' and 3' non-coding regions (NCR) of four dengue-2 viruses isolated from dengue patients with different clinical severities in Nakhon Phanom, Northeastern Thailand in 1993. The results were compared to prototype dengue-2 strains and were found to have highest homology with the New Guinea C strain. The sequence of the 5' NCR was completely conserved among all 4 isolates and were identical to the sequence of the New Guinea C strain. Homology of the 3' NCR sequence of the four isolates with the prototype strain ranged from 97.3 to 97.8%. Isolate ThNH-p11/93 from a mild dengue fever case showed the highest divergence from the prototype strain and the rest of the isolates from severe hemorrhagic cases, (1.11%). This includes a change in triad 297-299 nucleotides from the 3' terminus. Computer predicted secondary structures showed that isolate ThNHp-11/93 had significant structural differences from the other three isolates at this region.
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Japan.
authors.forename: M N
authors.initials: MN
authors.lastname: Mangada
authors.forename: A
authors.initials: A
authors.lastname: Igarashi
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013785:Thailand; D014774:Virulence / Q000235:genetics
chemical_list: D017931:DNA Primers; D012367:RNA, Viral