DPP controls tracheal cell migration along the dorsoventral body axis of the Drosophila embryo.
We report that DPP signaling is required for directed tracheal cell migration during Drosophila embryogenesis. The failure of tracheal cells to receive the DPP signal from adjacent dorsal and ventral cells results in the absence of dorsal and ventral migrations. Ectopic DPP signaling can reprogram cells in the center of the placode to adopt a dorsoventral migration behavior. The effects observed in response to ectopic DPP signaling are also observed upon the tracheal-specific expression of a constitutive active DPP type I receptor (TKV(Q253D)), indicating that the DPP signal is received and transmitted in tracheal cells to control their migration behavior. DPP signaling determines localized gene expression patterns in the developing tracheal placode, and is also required for the dorsal expression of the recently identified BRANCHLESS (BNL) guidance molecule, the ligand of the BREATHLESS (BTL) receptor. Thus, DPP plays a dual role during tracheal cell migration. It is required to control the dorsal expression of the BNL ligand; in addition, the DPP signal recruits groups of dorsal and ventral tracheal cells and programs them to migrate in dorsal and ventral directions.
Vincent
S
S
Abteilung Zellbiologie, Biozentrum, Universität Basel, Switzerland.
Ruberte
E
E
Grieder
N C
NC
Chen
C K
CK
Haerry
T
T
Schuh
R
R
Affolter
M
M
D029404:Activin Receptors; D000818:Animals; D002465:Cell Movement; D004268:DNA-Binding Proteins / Q000502:physiology; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology*; D004627:Embryonic Induction; D005346:Fibroblast Growth Factors; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances / Q000502:physiology*; D018398:Homeodomain Proteins / Q000502:physiology; D019476:Insect Proteins / Q000502:physiology*; D009024:Morphogenesis; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D011956:Receptors, Cell Surface / Q000502:physiology; D017978:Receptors, Growth Factor / Q000502:physiology*; D012097:Repressor Proteins / Q000502:physiology; D015398:Signal Transduction; D014132:Trachea / Q000196:embryology; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006133:Growth Substances; D018398:Homeodomain Proteins; D019476:Insect Proteins; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; D012097:Repressor Proteins; D014157:Transcription Factors; C102644:bnl protein, Drosophila; C064196:dpp protein, Drosophila; C074089:kni protein, Drosophila; C085793:salm protein, Drosophila; D005346:Fibroblast Growth Factors; C090169:tkv protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D029404:Activin Receptors