title: Identification of cDNAs encoding sterol methyl-transferases involved in the second methylation step of plant sterol biosynthesis.
abstract: Two methyl transfers are involved in the course of plant sterol biosynthesis and responsible for the formation of 24-alkyl sterols (mainly 24-ethyl sterols) which play major roles in plant growth and development. The first methyl transfer applies to cycloartenol, the second one to 24-methylene lophenol. Five cDNA clones encoding two Arabidopsis thaliana, two Nicotiana tabacum and one Ricinus communis S-adenosyl-L-methionine (AdoMet) sterol methyltransferases (SMT) were isolated. The deduced amino acid sequences of A. thaliana and N. tabacum SMT are about 80% identical in all possible combinations. In contrast they are about 40% identical with the deduced amino acid sequence of R. communis SMT and the published Glycine max sequence. Both A. thaliana and one N. tabacum SMT cDNAs were expressed in a yeast null mutant erg6, deficient in AdoMet zymosterol C24-methyltransferase and containing C24-non-alkylated sterols. In all cases, several 24-ethylidene sterols were synthesized. A thorough study of the sterolic composition of erg6 expressing the A. thaliana cDNA 411 (erg6-4118-pYeDP60) showed 24-methylene and 24-ethylidene derivatives of 4-desmethyl, 4alpha-methyl and 4,4-dimethyl sterols as well as 24-methyl and 24-ethyl derivatives of 4-desmethyl sterols. The structure of 5alpha-stigmasta-8, Z-24(24(1))-dien-3beta-ol, the major sterol of transformed yeasts, was demonstrated by 400 MHz 1H NMR. Microsomes from erg6-4118-pYeDP60 were shown to possess AdoMet-dependent sterol-C-methyltransferase activity. Delipidated preparations of these microsomes converted cycloartenol into 24-methylene cycloartanol and 24-methylene lophenol into 24-ethylidene lophenol, thus allowing the first identification of a plant sterol-C-methyltransferase cDNA. The catalytic efficiency of the expressed SMT was 17-times higher with 24-methylene lophenol than with cycloartenol. This result provides evidence that the A. thaliana cDNA 411 (and most probably the 3 plant SMT cDNAs presenting 80% identity with it) encodes a 24-methylene lophenol-C-24(1) methyltransferase catalyzing the second methylation step of plant sterol biosynthesis.
authors.affiliation: Institut de Biologie Moléculaire des Plantes, Département d'Enzymologie Cellulaire et Moléculaire, Institut de Botanique, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Bouvier-Navé
authors.forename: T
authors.initials: T
authors.lastname: Husselstein
authors.forename: T
authors.initials: T
authors.lastname: Desprez
authors.forename: P
authors.initials: P
authors.lastname: Benveniste
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary; D008401:Gas Chromatography-Mass Spectrometry; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013261:Sterols / Q000096:biosynthesis / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D018076:DNA, Complementary; D013261:Sterols; D008780:Methyltransferases