GnRH receptors and GnRH endocrine effects on luteoma cells.
An ovary implanted into the spleen of an ovariectomized rat develops into a luteinized tumor, growing in response to gonadotrophins. Previously, it was shown that in vivo Buserelin, a gonadotrophin-releasing hormone (GnRH) analog, inhibited tumor growth. To determine if GnRH had a direct effect on tumor cells, the presence of GnRH receptors as well as the endocrine effects of buserelin were studied on tumoral tissue. GnRH receptors were present in luteoma in similar concentrations and dissociation constant (Kd) to control estrous ovaries. In vivo treatment with buserelin did not modify luteoma GnRH receptors. In organ incubations, luteoma secreted significantly higher estradiol and lower progesterone than estrous ovaries; addition of buserelin did not modify steroid secretion. The same difference in basal steroid secretion between luteoma cells and luteal cells superovulated prepubertal ovaries was observed in cell cultures. Although luteinizing-hormone (LH)-stimulated progesterone in both kinds of cells, buserelin significantly inhibited LH-stimulated progesterone only in luteoma cells. These results describe clear differences in basal steroid secretion between tumoral and normal tissue. Furthermore, they show that luteoma possess GnRH receptors similar to those in normal ovarian tissue, and that GnRH analogs have endocrine effects on these cells. Therefore, a direct effect of buserelin on luteoma cells can be postulated.
Chamson-Reig
A
A
Instituto de Biología y Medicina Experimental, CONICET, Buenos Aires, Argentina.
Lux-Lantos
V
V
Tesone
M
M
Libertun
C
C
D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D002064:Buserelin / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004971:Estrus / Q000502:physiology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D007700:Kinetics; D018311:Luteoma / Q000378:metabolism*; D009924:Organ Culture Techniques; D010051:Ovarian Neoplasms / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011966:Receptors, LHRH / Q000378:metabolism*; D013480:Superovulation / Q000502:physiology; D014407:Tumor Cells, Cultured
D018931:Antineoplastic Agents, Hormonal; D007457:Iodine Radioisotopes; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D002064:Buserelin