title: Trophic effects of glicentin on rat small-intestinal mucosa in vivo and in vitro.
abstract: To define the role of glicentin the active site of enteroglucagon, we evaluated the trophic effects of recombinant rat glicentin on rat small intestine and IEC-6 cells. In vivo, a significant increase was observed in jejunal wet weight, protein content, DNA content, and alkaline phosphatase activity after the subcutaneous administration of 100 micrograms/kg per day of glicentin for 2 weeks. In the ileum, however, there were no significant differences between the control versus glicentin groups in any of these parameters. Ornithine decarboxylase (ODC) activity 3.5 h after an intraperitoneal injection of glicentin was increased in the jejunal mucosa, but not in the ileal mucosa. In vitro, glicentin, at a dose of more than 100 ng/ml, significantly increased both tritium-thymidine incorporation and the number of IEC-6 cells. These findings indicate that glicentin exerts direct trophic effects on the rat small-intestinal mucosa and on the rat small-intestinal cell line, IEC-6, and that this peptide appears to be an active site of enteroglucagon.
authors.affiliation: Second Department of Internal Medicine, Shiga University of Medical Science, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Myojo
authors.forename: T
authors.initials: T
authors.lastname: Tsujikawa
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Fujiyama
authors.forename: T
authors.initials: T
authors.lastname: Bamba
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001835:Body Weight; D002452:Cell Count; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D053771:Glicentin; D005934:Glucagon / Q000378:metabolism / Q000494:pharmacology*; D004763:Glucagon-Like Peptides / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects*; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology*; D011498:Protein Precursors / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010446:Peptide Fragments; D011498:Protein Precursors; D004763:Glucagon-Like Peptides; D053771:Glicentin; D004247:DNA; D005934:Glucagon; D000469:Alkaline Phosphatase