title: IL-1 beta enhances neurite regeneration from transected-nerve terminals of adult rat DRG.
abstract: We clarified the roles of IL-1 beta in peripheral neural regeneration after axotomy in a three-dimensional collagen gel culture system ranging from a single neurone to a dorsal root ganglion (DRG) explant with its associated nerve bundles. Application of 30 U/ml IL-1 beta to the culture systems clearly enhanced neural regeneration. This regeneration was evident in transected nerve terminals of DRG explants with or without associated nerve bundles, but not in dissociated single neurones. Neural survival was not affected by IL-1 beta in any of these culture systems. These results suggest that IL-1 beta stimulates surrounding non-neuronal cells to secrete neurotrophic factors, thus enhancing neurite regeneration from transected nerve terminals in cultured adult DRG explants.
authors.affiliation: Department of Physiology, School of Medicine, Yokohama City University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Horie
authors.forename: I
authors.initials: I
authors.lastname: Sakai
authors.forename: Y
authors.initials: Y
authors.lastname: Akahori
authors.forename: T
authors.initials: T
authors.lastname: Kadoya
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007375:Interleukin-1 / Q000494:pharmacology*; D009411:Nerve Endings / Q000187:drug effects / Q000502:physiology; D009416:Nerve Regeneration / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007375:Interleukin-1