[A new way for inbred strain mice genetic monitoring and the discovery of sex-linkaging RAPD markers].
We used 21 10 bp random primers to amplify DNA for four colonies of BALB/ c mice, four individuals of C 57 BL mice, and four individuals of Kunming stock mice. The amplified band patterns were different between BALB/c and C 57 BL mice in the products of 13 primers, and 8 primer products showed difference between BALB/c and Kunming stock mice. These results indicated that we can easily distinguish different strains of mice by RAPD method. For the four colonies of BALB/c mice, the genetic background of Chengdu colony. Shanghai colony and Beijing colony were homogeneous at all RAPD markers, however, in Kunming colony, there were two BALB/c individuals were disclosed different amplified patterns by 4 primers, showing that these two Kunming BALB/c mice maybe suffer genetic contamination or mutation some time. An unexpected phenomenon discovered in this study is that sex specific amplification bands were amplified by primer OPG 2, OPE 4, OPE 9 in all male mice. Although an 1.2 kb band were disclosed by both OPG 2 and OPE 4 in male mice, cross RAPD and not hybridization showed that the two bands were not homologous.
Zhang
W V
WV
Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming.
Gong
C M
CM
Wei
Y L
YL
Wu
C H
CH
Wang
W
W
Lang
H
H
Li
S R
SR
Su
B
B
Zhang
Y P
YP
D000818:Animals; D004247:DNA / Q000032:analysis; D005260:Female; D008040:Genetic Linkage; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics*; D008810:Mice, Inbred C57BL / Q000235:genetics*; D009154:Mutation; D019105:Random Amplified Polymorphic DNA Technique; D012730:Sex Chromosomes
D004247:DNA