ATP content measured by 31P NMR correlates with post-ischaemic recovery of external work output in an isolated rat heart perfused with erythrocyte suspension.
The limited capacity of the heart to survive ischaemia presents a recognized problem during open-heart surgery. In order to provide a baseline for the study of cardioprotection we subjected a series of isolated rat hearts to periods of ischaemia of increasing duration, and sought correlations between phosphate metabolite levels as measured by 11P NMR and post-ischaemic recovery of external work output. A strong linear correlation was found between the fraction of ATP measured after reperfusion expressed as a ratio. RATP to the pre-ischaemic level, and the fractional recovery of external work output, RW: Rw = 1.06 (SE 0.27) RATP + 0.01 (SE 0.21) (Spearman rank correlation coefficient 0.72, two-tailed P value 0.006).
Houston
R J
RJ
Physiology Department 237, Faculty of Medical Sciences, University of Nijmegen, The Netherlands.
Heerschap
A
A
Skotnicki
S H
SH
Verheugt
F W
FW
Oeseburg
B
B
D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002302:Cardiac Output; D004912:Erythrocytes / Q000502:physiology*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology; D015425:Myocardial Reperfusion / Q000379:methods*; D010759:Phosphorus Isotopes; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values
D010759:Phosphorus Isotopes; D000255:Adenosine Triphosphate