Diversity of the Escherichia coli type 1 fimbrial lectin. Differential binding to mannosides and uroepithelial cells.
Type 1 fimbriae are the most common adhesive organelles of Escherichia coli. Because of their virtual ubiquity, previous epidemiological studies have not found a correlation between the presence of type 1 fimbriae and urinary tract infections (UTIs). Recently it has become clear that type 1 fimbriae exhibit several different phenotypes, due to allelic variation of the gene for the lectin subunit, FimH, and that these phenotypes are differentially distributed among fecal and UTI isolates. In this study, we have analyzed in more detail the ability of isogenic, recombinant strains of E. coli expressing fimH genes of the predominant fecal and UTI phenotypes to adhere to glycoproteins and to uroepithelial cells. Evidence was obtained to indicate that type 1 fimbriae differ in their ability to recognize various mannosides, utilizing at least two different mechanisms. All FimH subunits studied to date are capable of mediating adhesion via trimannosyl residues, but only certain variants are capable of mediating high levels of adhesion via monomannosyl residues. The ability of the FimH lectins to interact with monomannosyl residues strongly correlates with their ability to mediate E. coli adhesion to uroepithelial cells. In this way, it would be possible for certain phenotypic variants of type 1 fimbriae to contribute more than others to virulence of E. coli in the urinary tract.
Sokurenko
E V
EV
Department of Anatomy & Neurobiology, University of Tennessee, Memphis, Tennessee 38163, USA.
Chesnokova
V
V
Doyle
R J
RJ
Hasty
D L
DL
D018829:Adhesins, Bacterial / Q000378:metabolism*; D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion; D001665:Binding Sites; D002417:Cattle; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000378:metabolism* / Q000472:pathogenicity / Q000648:ultrastructure; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000378:metabolism*; D008362:Mannosides / Q000378:metabolism*; D008854:Microscopy, Electron; D011995:Recombination, Genetic; D012260:Ribonucleases / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014551:Urinary Tract / Q000166:cytology / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000382:microbiology
D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D037102:Lectins; D008362:Mannosides; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; D012710:Serum Albumin, Bovine; D012260:Ribonucleases; C029600:ribonuclease B