title: Malignant lymphoma induction in rabbits by oral inoculation of crude virus fraction prepared from Ts-B6 cells (cynomolgus B-lymphoblastoid cells harboring Epstein-Barr virus-related simian herpesvirus).
abstract: Malignant lymphoma was induced in Japanese (JWY), New Zealand (NZY) and Dutch (DUY) white rabbits by oral spray of cell-free pellets of culture fluid (crude virus fraction) of Ts-B6 cells (cynomolgus monkey B-lymphoblastoid cells harboring Epstein Barr virus-related simian herpesvirus or Cyno-EBV). Nine of 11 inoculated rabbits developed malignant lymphomas within 42-160 days after oral inoculation (JWY, 2/3; NZY, 5/6; DUY, 2/2). In contrast, none of the control rabbits inoculated in the same fashion with B95-8 (EBV-producing marmoset cell line) cell-free pellets developed malignant lymphoma. Most rabbits showed increased anti-VCA IgG and anti-EA-DR IgG antibody titers after inoculation by oral spray of Ts-B6 cell-free pellets. EBV-encoded RNA-1 was revealed in the tumor cells by in situ hybridization. EBV DNA was detected in the rabbit peripheral blood leukocytes (PBL) by polymerase chain reaction; the earliest positive result was obtained only two days after oral inoculation. These data suggest that orally administered Cyno-EBV in Ts-B6 cells infects PBL and then induces malignant lymphoma in rabbits. The availability of this animal model promises to clarify the role of EBV in human lymphoma and provides a means for studying prophylactic and therapeutic regimens.
authors.affiliation: Second Department of Pathology, Okayama University Medical School, Japan.
authors.forename: H L
authors.initials: HL
authors.lastname: Chen
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: T R
authors.initials: TR
authors.lastname: Koirala
authors.forename: H
authors.initials: H
authors.lastname: Ino
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: C R
authors.initials: CR
authors.lastname: Choudhury
authors.forename: T
authors.initials: T
authors.lastname: Akagi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000097:blood / Q000276:immunology; D002213:Capsid / Q000097:blood / Q000276:immunology; D036022:Capsid Proteins; D002472:Cell Transformation, Viral; D002474:Cell-Free System; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D017403:In Situ Hybridization; D016393:Lymphoma, B-Cell / Q000209:etiology* / Q000473:pathology / Q000821:virology*; D008252:Macaca fascicularis; D009368:Neoplasm Transplantation; D012367:RNA, Viral / Q000737:chemistry; D011817:Rabbits; D014407:Tumor Cells, Cultured
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C041788:Epstein-Barr viral capsid antigen; C033305:Epstein-Barr virus early antigen; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral