title: PCR-mediated detection of acidophilic, bioleaching-associated bacteria.
abstract: The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg.
authors.affiliation: Department of Microbiology, University of Queensland, Brisbane, Australia.
authors.forename: P
authors.initials: P
authors.lastname: De Wulf-Durand
authors.forename: L J
authors.initials: LJ
authors.lastname: Bryant
authors.forename: L I
authors.initials: LI
authors.lastname: Sly
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D008906:Mining; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S