Determination of acyclovir in human plasma by high-performance liquid chromatography.
A selective and sensitive isocratic high-performance liquid chromatographic method for the analysis of acyclovir in human plasma was described. Acid deproteinisation was used as sample treatment. Mean analytical recoveries were higher than 94% at low and high concentrations. The quantification limit was 0.1 mg/l for a plasma volume of 500 microl and precision study exhibits coefficients of variation lower than 5%. The method is suitable for therapeutic monitoring of acyclovir concentrations in organ-transplant recipients.
Boulieu
R
R
Institut des Sciences Pharmaceutiques et Biologiques, Laboratoire de Pharmacie Clinique et d'Evaluation du Médicament, Lyon, France.
Gallant
C
C
Silberstein
N
N
D000212:Acyclovir / Q000097:blood*; D000998:Antiviral Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012680:Sensitivity and Specificity
D000998:Antiviral Agents; D000212:Acyclovir