Domains within the Vibrio cholerae toxin coregulated pilin subunit that mediate bacterial colonization.
Several experimental approaches have provided evidence suggesting that a domain within the C-terminal region of the TcpA pilin, delineated by the single disulfide loop, is directly responsible for the colonization function mediated by the toxin coregulated pilus (TCP) of Vibrio cholerae. This evidence includes the mapping of domains recognized by protective monoclonal antibodies to this region, the ability of peptides from within this region to elicit cholera protective antibody, the construction of tcpA missense mutations that abolish TCP function, and the requirement of a periplasmic disulfide isomerase to produce functional TCP.
Sun
D
D
Department of Microbiology, Dartmouth Medical School, Hanover, NH 03755, USA.
Lafferty
M J
MJ
Peek
J A
JA
Taylor
R K
RK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002771:Cholera / Q000517:prevention & control; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000378:metabolism / Q000502:physiology*; D007116:Immunization, Passive; D007535:Isomerases / Q000378:metabolism; D051379:Mice; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D011487:Protein Conformation; D019704:Protein Disulfide-Isomerases; D014734:Vibrio cholerae / Q000737:chemistry* / Q000472:pathogenicity* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004220:Disulfides; C085807:TcpA protein, Vibrio cholerae; D036781:Fimbriae Proteins; D007535:Isomerases; D019704:Protein Disulfide-Isomerases; C106180:TcpG protein, Vibrio cholerae