title: Increased yield of F VIII from pooled plasma vs. single-donor plasma in the production of cryoprecipitates.
abstract: Coagulation factor VIII:C yield was studied in two types of cryoprecipitates. The first group contained products from single-donor plasma units. The other group contained cryoprecipitates which were produced from pooled plasma. The volume of plasma/bag was not different between the two groups, but both the yield and the total content of F VIII:C in cryoprecipitates were significantly different. The yield of F VIII:C was higher (+20% in relative terms) in cryoprecipitates produced from pooled plasma, resulting in higher potency of such products. The positive effect of plasma pooling on the recovery of F VIII:C might be a result of reassembly of factor VIII subunits of different individuals in the plasma pools. The findings may have a role also in large-scale production of F VIII concentrates.
authors.affiliation: National Blood Center of Hungary, Budapest, Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Rácz
authors.forename: M
authors.initials: M
authors.lastname: Thék
authors.forename: A
authors.initials: A
authors.lastname: Jánossy
authors.forename: J
authors.initials: J
authors.lastname: Pintér
authors.forename: C
authors.initials: C
authors.lastname: Baróti
mesh_terms: D011232:Chemical Precipitation; D005069:Evaluation Studies as Topic; D005169:Factor VIII / Q000302:isolation & purification*; D005615:Freezing; D006801:Humans; D010949:Plasma / Q000737:chemistry*
chemical_list: D005169:Factor VIII