Assessment of mutagenic potential of thiram.
Thiram is a widely used dithiocarbamate fungicide. In this study, the mutagenicity of thiram was investigated using the micronucleus and dominant lethal tests in Swiss albino mice. A single ip injection of 100 mg thiram/kg body weight, which is the maximum tolerated dose (MTD), significantly induced micronucleus formation in bone marrow cells after 30 and 48 hr of exposure; 50% and 25% of the MTD also induced micronucleus formation after the above time periods. A significant number of dead implants were induced when thiram was given to male mice in the diet at 10% of the oral LD50 during the whole spermatogenesis cycle (8 wk); this post-implantation loss indicates a dominant lethal mutation.
Agrawal
R C
RC
Environmental Carcinogenesis Laboratory, Industrial Toxicology Research Centre, Lucknow, India.
Shukla
Y
Y
Mehrotra
N K
NK
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D047108:Embryonic Development; D005260:Female; D005659:Fungicides, Industrial / Q000633:toxicity*; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009154:Mutation / Q000187:drug effects*; D011247:Pregnancy; D013091:Spermatogenesis / Q000187:drug effects; D013893:Thiram / Q000633:toxicity*
D005659:Fungicides, Industrial; D013893:Thiram