DNA sequencing and analysis of 130 kb from yeast chromosome XV.
We have determined the nucleotide sequence of 129,524 bases of yeast (Saccharomyces cerevisiae) chromosome XV. Sequence analysis revealed the presence of 59 non-overlapping open reading frames (ORFs) of length > 300 bp, three tRNA genes, four delta elements and one Ty-element. Among the 21 previously known yeast genes (36% of all ORFs in this fragment) were nucleoporin (NUP1), ras protein (RAS1), RNA polymerase III (RPC1) and elongation factor 2 (EF2). Further, 31 ORFs (53% of the total) were found to be homologous to known protein or DNA sequences, or sequence patterns. For seven ORFs (11% of the total) no homology was found. Among the most interesting protein identification in this DNA fragment are an inositol polyphosphatase, the second gene of this type found in yeast (homologous to the human OCRL gene involved in Lowe's syndrome), a new ADP ribosylation factor of the arf6 subfamily, the first protein containing three C2 domains, and an ORF similar to a Bacillus subtilis cell-cycle related protein. For each ORF detailed sequence analysis was carried out, with a full consideration of its biological function and pointing out key regions of interest for further functional analysis.
Voss
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H
Biochemical Instrumentation Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Benes
V
V
Andrade
M A
MA
Valencia
A
A
Rechmann
S
S
Teodoru
C
C
Schwager
C
C
Paces
V
V
Sander
C
C
Ansorge
W
W
D020727:ADP-Ribosylation Factors; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D015825:Chromosomes, Fungal / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics; D024002:HMGB Proteins; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000235:genetics; D016366:Open Reading Frames; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012320:RNA Polymerase III / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D055748:SOXB1 Transcription Factors; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D018631:ras Proteins / Q000235:genetics
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D024002:HMGB Proteins; D008565:Membrane Proteins; C064550:NUP1 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D011506:Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012343:RNA, Transfer; D012320:RNA Polymerase III; D010744:Phosphoric Monoester Hydrolases; C021822:myo-inositol-1 (or 4)-monophosphatase; C075695:OCRL protein, human; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors; C489014:RAS1 protein, S cerevisiae; D018631:ras Proteins