Detection of parvovirus B19-specific IgM by antibody capture radioimmunoassay.
A solid phase IgM-capture radioimmunoassay (MACRIA) for the detection of parvovirus B19-IgM is described (Cohen et al., 1983, J. Hyg. Camb. 91, 113-130). IgM from a dilution of patients serum is 'captured' onto a solid phase coated by anti-human IgM. To determine whether any of the IgM is specific for parvovirus B19, B19 antigen is added followed by a detector system. In the MACRIA described here the detector system comprises a mouse monoclonal antibody to parvovirus B19 and a 125I-labelled anti-mouse antibody. A calibration curve derived from a standard B19 IgM serum is used to quantify B19 IgM using a single dilution of test sera. The purpose of the protocol is the diagnosis of recent acute infection with parvovirus B19.
Cohen
B J
BJ
Enteric and Respiratory Virus Laboratory, Central Public Health Laboratory, London, UK. bcohen@phls.co.uk
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D016732:Parvovirus B19, Human / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay
D000914:Antibodies, Viral; D007075:Immunoglobulin M