Evaluation of anti-AIDS drugs in conventional mice implanted with a permeable membrane device containing human T cells infected with HIV.
We now report the confirmation of the work of Hollingshead et al. (1995) on development of a cell based hollow fiber (HF) system for evaluating potential anti-AIDS drugs in vivo using conventional mice rather than SCID mice. CD4 +, CEM-SS cells infected with HIV/1, strain RF, at a multiplicity of infection of 0.1 were placed into HFs. The fibers were implanted into the peritoneal cavity of outbred Swiss mice. Using this model, the antiviral activity of azidothymidine (AZT) at doses of approximately 150, 75 and 37.5 mg/kg/day was evaluated by administering AZT to the mice in drinking water. Upon fiber removal on day 6, AZT treatment was shown to significantly increase CEM cell viability over the untreated, virus control group and significantly reduced the levels of HIV p24 and HIV RT activity.
Quenelle
D C
DC
Life Sciences Division, Southern Research Institute, Lexington, MA 02173, USA.
Keith
K A
KA
Dunleavy
K E
KE
Taylor
B A
BA
Bowdon
B J
BJ
Brazier
A D
AD
Mullon
C J
CJ
HarrisRE
Allen
L B
LB
D000180:Acrylic Resins; D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000637:transplantation / Q000821:virology*; D002470:Cell Survival; D003588:Cytopathogenic Effect, Viral; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D016655:HIV Core Protein p24 / Q000032:analysis; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D010539:Permeability; D011108:Polymers; D011143:Polyvinyl Chloride; D019736:Prostheses and Implants; D015215:Zidovudine / Q000494:pharmacology
D000180:Acrylic Resins; D019380:Anti-HIV Agents; D016655:HIV Core Protein p24; D008567:Membranes, Artificial; D011108:Polymers; C086656:polyacrylonitrile-polyvinylchloride; D015215:Zidovudine; D011143:Polyvinyl Chloride; D054303:HIV Reverse Transcriptase