title: Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.
abstract: In this report, we show that the vasoactive peptide agonist bradykinin (BK) when bound to B2 BK receptors on DDT1 MF-2 smooth muscle cells promotes the recruitment and sequestration of the occupied receptors and the receptor-coupled G-protein alpha subunits Galphaq and Galphai in caveolae. Association of ligand receptor complexes and Galpha subunits with caveolae was indicated by their co-enrichment on density gradients with caveolin, a marker protein for caveolae. Caveolin and Galpha subunits were monitored by immunoblotting, whereas receptors were monitored as ligand receptor complexes formed by labeling receptors with the agonist BK or the antagonist NPC17731 prior to cell disruption and caveolae enrichment. These complexes were detected with radioligand and by immunoblotting with BK antibodies. A direct interaction of Galpha subunits with caveolin was also indicated by their co-immunoprecipitation. Immunoelectron microscopy revealed that the enriched caveolin, Galpha subunits, and BK receptor complexes were present in structures of 0.1-0.2 microm. At 4 degrees C, BK and NPC17731 receptor complexes were detected in caveolae, and both complexes were sensitive to acid washing prior to cell disruption and caveolae enrichment. Elevation of the temperature to 37 degrees C increased the amount of BK receptor complexes in caveolae with a maximal response at 10 min (continuous labeling) or 20 min (single-round labeling), and the complexes became acid-resistant. These conditions also increased the amount of Galphaq and Galphai in caveolae with a maximal response at 5-10 min. In contrast, the NPC17731 receptor complexes remained acid-sensitive and dissociated at this temperature, and antagonists did not increase the amount of Galpha subunits in caveolae. These results show that some agonists that act through G-protein-coupled receptors promote the association of their receptors and receptor-coupled Galpha subunits with caveolae.
authors.affiliation: Department of Biochemistry, The University of Texas Health Science Center, San Antonio, Texas 78284-7760, USA.
authors.forename: W F
authors.initials: WF
authors.lastname: de Weerd
authors.forename: L M
authors.initials: LM
authors.lastname: Leeb-Lundberg
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000378:metabolism*; D065168:Bradykinin Receptor Antagonists; D002451:Cell Compartmentation; D002478:Cells, Cultured; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D016253:Microscopy, Immunoelectron; D009130:Muscle, Smooth / Q000378:metabolism* / Q000648:ultrastructure; D009842:Oligopeptides / Q000378:metabolism; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D065168:Bradykinin Receptor Antagonists; D009842:Oligopeptides; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; C071327:NPC 17731; D019204:GTP-Binding Proteins; D001920:Bradykinin