title: The role of human leukocyte antigen in susceptibility and clinical manifestations of sarcoidosis.
abstract: OBJECTIVE
  To investigate the correlation of human leukocyte antigen (HLA) with susceptibility and clinical manifestations of sarcoidosis.
  
  
  METHODS
  Fifty-five patients with sarcoidosis and one hundred and six normal subjects were investigated. Genomic DNAs were purified using the proteinase K-phenol extraction method. DNA samples were amplified by the polymerase chain reaction (PCR) procedure using the DRB1 group specific primer pairs.
  
  
  RESULTS
  The gene frequency of HLA-DR5 increased significantly in patients with sarcoidosis (P < 0.01), and HLA-DR7 decreased (P < 0.05). Gene frequencies of HLA-DR9 and HLA-DR5 increased remarkably in male patients and in patients with stage I and stage IIa, respectively (both P < 0.05).
  
  
  CONCLUSIONS
  It is suggested that HLA-DR gene might contribute to the susceptibility as well as various clinical manifestations of sarcoidosis.
authors.affiliation: Department of Respiratory Medicine, Peking Union Medical College (PUMC) Hospital, Beijing.
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: C
authors.initials: C
authors.lastname: Qiu
authors.forename: W
authors.initials: W
authors.lastname: Luo
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004198:Disease Susceptibility; D005260:Female; D005787:Gene Frequency; D015805:HLA-DR5 Antigen / Q000235:genetics / Q000502:physiology*; D015809:HLA-DR7 Antigen / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017565:Sarcoidosis, Pulmonary / Q000235:genetics*
chemical_list: D015805:HLA-DR5 Antigen; D015809:HLA-DR7 Antigen