Inhibitory effect of ethanol on the Na(+)-ATPase activity of rat kidney proximal tubular cell plasma membranes.
The inhibitory effect of 2% ethanol (400 mM) in the incubation medium on several characteristics of the Na(+)-ATPase of basolateral plasma membranes from rat kidney proximal tubular cells was investigated. Ethanol did not change the Km of the enzyme for Mg2+, ATP or Na+; it did not change either the optimal pH or temperature values of the incubation medium for the enzyme to act and finally, it did not affect the apparent energy of activation of the enzyme. It was also found that 2% ethanol produced stronger inhibition of the ATPase when it is in an activated or stimulated state, than when it is working at its lower basal level. The presented results can be explained by assuming that 2% ethanol in the incubation medium inhibits Na(+)-ATPase activity by affecting the enzyme structure as well as its activating mechanism.
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Centre of Biophysics and Biochemistry, Institute of Scientific Research, Caracas, Venezuela.
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D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D027682:Cation Transport Proteins; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007038:Hypotonic Solutions; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D027682:Cation Transport Proteins; D004791:Enzyme Inhibitors; D007038:Hypotonic Solutions; D007552:Isotonic Solutions; D000431:Ethanol; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase; D008274:Magnesium