title: Relationship between the type of BCR-ABL rearrangement and bone marrow histopathological features in chronic myeloid leukemia.
abstract: The aim of the present study was to characterize the type of BCR-ABL transcript and to correlate the molecular feature with bone marrow histology. For this purpose, we analysed the BCR-ABL rearrangement in 26 patients with chronic myeloid leukemia (CML) in the chronic phase by RT-PCR, and we also classified the bone marrow histology according to the predominance of granulocylic (GRAN) or granulocytic and megakaryocytic (GRAM/MEG) proliferation, after analysis of two independent observers. We did not find any significant difference in survival of patients presenting b2-a2 and b3-a2 transcripts or GRAN and GRAN/MEG bone marrow types, nor did we find any significant correlation of the type of BCR-ABL transcript with the bone marrow histological subgroups GRAN and GRAN-MEG (Fisher's test = 0.31). Thus, we conclude that the presence of exon b3 is not correlated to bone marrow histology in CML.
authors.affiliation: Divisão de Hematologia, Universidade Federal de São Paulo, Brazil.
authors.forename: G W
authors.initials: GW
authors.lastname: Colleoni
authors.forename: M R
authors.initials: MR
authors.lastname: Silva
authors.forename: R S
authors.initials: RS
authors.lastname: Silva
authors.forename: F F
authors.initials: FF
authors.lastname: Costa
authors.forename: J
authors.initials: J
authors.lastname: Kerbauy
authors.forename: S T
authors.initials: ST
authors.lastname: Saad
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000473:pathology*; D005091:Exons; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D015466:Leukemia, Myeloid, Chronic-Phase / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011379:Prognosis
chemical_list: D016044:Fusion Proteins, bcr-abl