How important is the N-3 sugar moiety in the tight-binding interaction of coformycin with adenosine deaminase?
Preliminary findings on the possible important role of the N-3 sugar moiety of coformycin in its tight-binding interaction with adenosine deaminase (ADA) are reported. The compound 3-beta-D-Ribofuranosyl-5,6,7,8-tetrahydro-4H-imidazo[4,5-d][1,3]diaze pin-5-one-8-ol (1), its 3-benzyl analogue (6), and the aglycon (7) served as probes. The first two were both found to be competitive inhibitors of ADA with Ki's in the range of 10(-5) M, while the last one was inactive.
Hosmane
R S
RS
Department of Chemistry & Biochemistry, University of Maryland Baltimore County, Baltimore 21250, USA.
Hong
M
M
D000243:Adenosine Deaminase / Q000737:chemistry*; D001665:Binding Sites; D002241:Carbohydrates / Q000737:chemistry; D003070:Coformycin / Q000737:chemistry*; D008958:Models, Molecular; D011485:Protein Binding
D002241:Carbohydrates; D003070:Coformycin; D000243:Adenosine Deaminase