Immune responses in goats to caprine arthritis-encephalitis virus.
In this study, goats were experimentally infected with caprine arthritis-encephalitis virus (CAEV). Anti-CAEV antibodies were detected in vivo using indirect enzyme-linked immunosorbent assay (ELISA) and Western blot assays. The results showed that the sensitivity of detection of anti-CAEV antibodies using indirect ELISA was relatively high, whereas Western blotting was very sensitive for detection of P28 anti-CAEV antibody as 4 days postinfection. Apparently, because of high immunogenicity of P28 antigen, the detection of antibodies specific for this viral protein may represent a valuable assay to detect early infection. CAEV related to other lentiviruses may be useful in studies of human immunodeficiency virus (HIV) and related viruses in animal models.
Ding
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Institute of Genetics, Fudan University, Shanghai, The People's Republic of China.
Xiang
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D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D016088:Arthritis-Encephalitis Virus, Caprine / Q000276:immunology*; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology / Q000821:virology; D006041:Goats; D016180:Lentivirus Infections / Q000276:immunology* / Q000662:veterinary; D012692:Serial Passage; D013583:Synovial Membrane / Q000166:cytology / Q000821:virology; D014779:Virus Replication
D000914:Antibodies, Viral