The isomeric preference of Holliday junctions influences resolution bias by lambda integrase.
Lambda site-specific recombination proceeds by a pair of sequential strand exchanges that first generate and then resolve a Holliday junction intermediate. A family of synthetic Holliday junctions with the branch point constrained to the center of the 7 bp overlap region was used to show that resolution of the top strands and resolution of the bottom strands are symmetrical but stereochemically distinct processes. Lambda integrase is sensitive to isomeric structure, preferentially resolving the pair of strands that are crossed in the protein-free Holliday junction. At the branch point of stacked immobile Holliday junctions, the number of purines is preferentially maximized in the crossed (versus continuous) strands if there is an inequality of purines between strands of opposite polarity. This stacking preference was used to anticipate the resolution bias of freely mobile junctions and thereby to reinforce the conclusions with monomobile junctions. The results provide a strong indication that in the complete recombination reaction a restacking of helices occurs between the top and bottom strand exchanges.
Azaro
M A
MA
Division of Biology and Medicine, Brown University, Providence, RI 02912, USA.
Landy
A
A
D010582:Bacteriophage lambda / Q000201:enzymology*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004274:DNA, Recombinant / Q000737:chemistry / Q000378:metabolism; D019426:Integrases / Q000378:metabolism*; D007536:Isomerism; D009690:Nucleic Acid Conformation; D011687:Purines
D004274:DNA, Recombinant; D011687:Purines; D004247:DNA; D019426:Integrases