title: Periodate treatment of Anisakis simplex allergens.
abstract: Anaphylactic reactions after parasitized fish consumption are mediated by an IgE response. However, positive skin tests and specific IgE can also be found in many asymptomatic subjects who recognize a single medium-mol.-wt. antigen by IgE immunoblot. The study aimed to find out whether this unspecificity was due to the carbohydrate moieties of parasite antigens. Sixty-two patients with suspected parasite allergy, 51 blood donors, 18 bakers, and 38 atopic patients were studied by blotting. Parasite proteins were treated with periodate. Several selected sera were inhibited with a crude wheat extract and fungal amylase. Twelve patients (19%), eight donors (16%), six bakers (33%), and one atopic patient (3%) recognized a single medium-mol.-wt. band in blotting and should be considered false-positive. This band was periodate-sensitive, but specific IgE to this allergen could not be inhibited by a wheat extract nor by fungal amylase and was clinically irrelevant. Diagnosis of Anisakis simplex hypersensitivity by skin tests and/ or specific IgE values should always be confirmed by specific IgE immunoblotting in order to detect the presence of clinically unrelated antibodies directed to periodate-sensitive allergens. These allergens are probably not a carbohydrate moiety of a parasite glycoprotein.
authors.affiliation: Centro de Investigación Clínica, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Moneo
authors.forename: M T
authors.initials: MT
authors.lastname: Audicana
authors.forename: E
authors.initials: E
authors.lastname: Alday
authors.forename: G
authors.initials: G
authors.lastname: Curiel
authors.forename: M D
authors.initials: MD
authors.lastname: del Pozo
authors.forename: M
authors.initials: M
authors.lastname: García
mesh_terms: D000681:Amylases / Q000276:immunology; D000818:Animals; D017163:Anisakis / Q000276:immunology*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis* / Q000276:immunology*; D005399:Fishes / Q000469:parasitology; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D010504:Periodic Acid; D010936:Plant Extracts / Q000276:immunology; D017747:Seafood / Q000009:adverse effects; D012882:Skin Tests
chemical_list: D000947:Antigens, Helminth; D002241:Carbohydrates; D010936:Plant Extracts; D010504:Periodic Acid; D007073:Immunoglobulin E; C009288:metaperiodate; D000681:Amylases