Sex chromosome markers: characterization using fluorescence in situ hybridization and review of the literature.
Fluorescence in situ hybridization (FISH) using biotin labeled X- and Y-chromosome DNA probes was utilized in the analysis of 23 sex chromosome-derived markers. Specimens were obtained through prenatal diagnosis, because of a presumptive diagnosis of Ullrich-Turner syndrome, mental retardation, and minor anomalies or ambiguous genitalia; three were spontaneous abortuses. Twelve markers were derived from the X chromosome and eleven from the Y chromosome; this demonstrates successfully the value and necessity of FISH utilizing DNA probes in the identification of sex chromosome markers. Both fresh and older slides, some of which had been previously G-banded, were used in these determinations. We have also reviewed the literature on sex chromosome markers identified using FISH.
Schwartz
S
S
Center for Human Genetics, Department of Genetics, Case Western Reserve University, and University Hospitals of Cleveland, Ohio 44106, USA.
Depinet
T W
TW
Leana-Cox
J
J
Isada
N B
NB
Karson
E M
EM
Park
V M
VM
Pasztor
L M
LM
Sheppard
L C
LC
Stallard
R
R
Wolff
D J
DJ
Zinn
A B
AB
Zurcher
V L
VL
Zackowski
J L
JL
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012730:Sex Chromosomes; D014424:Turner Syndrome / Q000235:genetics
D005819:Genetic Markers