Stabilization of lignin peroxidases in white rot fungi by tryptophan.
Supplementation of various cultures of white rot fungi with tryptophan was found to have a large stimulatory effect on lignin peroxidase activity levels. This enhancement was greater than that observed in the presence of the lignin peroxidase recycling agent veratryl alcohol. Using reverse transcription-PCR, we found that tryptophan does not act to induce lignin peroxidase expression at the level of gene transcription. Instead, the activity enhancement observed is likely to result from the protective effect of tryptophan against H2O2 inactivation. In experiments using a partially purified lignin peroxidase preparation, tryptophan and its derivative indole were determined to function in the same way as veratryl alcohol in converting compound II, an oxidized form of lignin peroxidase, to ferric enzyme, thereby completing the catalytic cycle. Furthermore, tryptophan was found to be a better substrate for lignin peroxidase than veratryl alcohol. Inclusion of either tryptophan or indole enhanced the oxidation of the azo dyes methyl orange and Eriochrome blue black. Stimulation of azo dye oxidations by veratryl alcohol has previously been shown to be due to its enzyme recycling function. Our data allow us to propose that tryptophan stabilizes lignin peroxidase by acting as a reductant for the enzyme.
Collins
P J
PJ
Microbiology Department, University College, Cork, Ireland.
Field
J A
JA
Teunissen
P
P
Dobson
A D
AD
D001391:Azo Compounds / Q000378:metabolism; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D001592:Benzyl Alcohols / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D006861:Hydrogen Peroxide / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic; D014364:Tryptophan / Q000378:metabolism*
D001391:Azo Compounds; D001592:Benzyl Alcohols; D003470:Culture Media; D007211:Indoles; C100258:methyl orange; C030374:indole; D014364:Tryptophan; D006861:Hydrogen Peroxide; D010544:Peroxidases; C042858:lignin peroxidase; C042197:veratryl alcohol