Hapten-specific tolerance induced by acute, low-dose ultraviolet B radiation of skin is mediated via interleukin-10.
Because interleukin (IL)-10 is an immunoregulatory cytokine that is produced by keratinocytes exposed to UVB radiation (UVR), we determined whether IL-10 participates in either failed contact hypersensitivity (CH) induction or tolerance after acute, low-dose UVR. Murine recombinant IL-10 (200 ng) was injected intradermally on shaved abdominal skin. To assess the effects of IL-10 on CH induction, dinitrofluorobenzene (DNFB, 185 microg) was painted on the skin within 30 min after IL-10 was injected, and the mice were assayed 5 d later by ear challenge with dilute DNFB. To assess tolerance, DNFB (185 microg) was painted a second time on normal body-wall skin 14 d after DNFB was first painted on IL-10-injected skin; CH was then assayed on day 19. We found that mice that received DNFB on IL-10-injected skin developed CH comparable in intensity to that observed in PBS-injected controls. Thus, this dose of IL-10 did not prove to be deleterious to CH induction if hapten was painted on the injected site within 30 min. By contrast, mice that first experienced DNFB within 30 min, 1 d, or 3 d after IL-10 had been injected intracutaneously displayed hapten-specific tolerance. Moreover, intraperitoneally injected anti-IL-10 antibody prevented UVR- and cis-urocanic acid-dependent tolerance; anti-IL-10 antibody had no effect on TNF-alpha-induced tolerance and failed to restore CH induction after UVR exposures. These data indicate that IL-10 is an important mediator of the tolerance induced when hapten is painted on the skin of animals exposed to acute, low-dose UVR.
Niizeki
H
H
Schepens Eye Research Institute and Department of Dermatology, Harvard Medical School, Boston, Massachusetts 02114, U.S.A.
Streilein
J W
JW
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology; D004139:Dinitrofluorobenzene; D000939:Epitopes; D006241:Haptens / Q000276:immunology; D007108:Immune Tolerance / Q000187:drug effects / Q000502:physiology; D016753:Interleukin-10 / Q000494:pharmacology*; D051379:Mice; D009500:Neutralization Tests; D012867:Skin / Q000276:immunology* / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000906:Antibodies; D000939:Epitopes; D006241:Haptens; D016753:Interleukin-10; D004139:Dinitrofluorobenzene