title: Effectiveness of leukocyte filters in reducing tumor cell contamination after intraoperative blood salvage in lung cancer patients.
abstract: OBJECTIVE
  In an effort to reduce allogeneic blood transfusions in patients undergoing elective surgery for lung cancer, we investigated the effectiveness of a method of processing shed blood with an automated device for intra operative blood salvage (IOBS) and filtration with a 3rd-generation polyester filter to remove tumor cells.
  
  
  METHODS
  Sixteen patients were operated on for different types of lung cancer. We searched for malignant cells in pre- and postprocessed shed blood employing density gradient centrifugation, staining of cytospins with hematoxylin-eosin, and antibodies to human cytokeratins.
  
  
  RESULTS
  In 9 out of 16 cases (56%), neoplastic cells were detected in prefiltration samples, but none were found in postfiltration cytospins.
  
  
  CONCLUSIONS
  IOBS combined with appropriate filtration could be a very useful and safe tool in reducing allogeneic blood transfusion in cancer patients.
authors.affiliation: Servizio Trasfusionale, Azienda Ospedaliera Ospedale E. Morelli, Sondalo, Italia.
authors.forename: P
authors.initials: P
authors.lastname: Perseghin
authors.forename: M
authors.initials: M
authors.lastname: Viganò
authors.forename: G
authors.initials: G
authors.lastname: Rocco
authors.forename: C
authors.initials: C
authors.lastname: Della Pona
authors.forename: A
authors.initials: A
authors.lastname: Buscemi
authors.forename: A
authors.initials: A
authors.lastname: Rizzi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001803:Blood Transfusion / Q000706:statistics & numerical data; D001804:Blood Transfusion, Autologous / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D002499:Centrifugation, Density Gradient; D005069:Evaluation Studies as Topic; D005260:Female; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D007430:Intraoperative Care / Q000295:instrumentation / Q000379:methods*; D007962:Leukocytes; D008175:Lung Neoplasms / Q000097:blood* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating; D011091:Polyesters
chemical_list: D011091:Polyesters