CHO transfectants produce large amounts of recombinant protein in suspension culture.
Chinese hamster ovary (CHO) cells transfected with various genes are widely used as adherent cell monolayers to produce recombinant proteins. In this report we present a new culture technique for CHO cells transfected with the vector pPOL-DHFR-CD14 using a minifermenter (miniPERM, Heraeus) for the production of recombinant human endotoxin receptor CD14 (rCD14). The transfectants were cultured for 12-17 days under serum-free conditions and formed spheroids. From this system we harvested supernatants containing up to 3.1 mg/ml recombinant CD14 (rCD14). This represents a 200-fold increase of rCD14 yield compared to conventional adherent CHO cell culture.
Schütt
C
C
Institute of Immunology and Transfusion Medicine, Ernst-Moritz-Arndt-University of Greifswald, Germany.
Fürll
B
B
Stelter
F
F
Jack
R S
RS
Witt
S
S
D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D018929:Cell Culture Techniques; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D018950:Lipopolysaccharide Receptors; D011994:Recombinant Proteins