title: A rapid bioassay to detect mycotoxins using a melanin precursor overproducer mutant of the ciliate Tetrahymena thermophila.
abstract: Four different mycotoxins (patulin, T-2 toxin, diacetoxyscirpenol and roquefortine) were used to study growth inhibitory effects on a melanin precursor overproducer mutant of the ciliate Tetrahymena thermophila. This strain is especially sensitive to diacetoxyscirpenol and T-2 toxin. The secretion capacity of melanin precursors into the culture medium by this mutant and its biosensor capacity are very useful characteristics to elaborate a rapid bioassay to detect some specific mycotoxins.
authors.affiliation: Departamento de Microbiologia-III, Facultad de Biologia, Universidad Complutense (UCM), Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martín González
authors.forename: L
authors.initials: L
authors.lastname: Benítez
authors.forename: T
authors.initials: T
authors.lastname: Soto
authors.forename: J
authors.initials: J
authors.lastname: Rodriguez de Lecea
authors.forename: J C
authors.initials: JC
authors.lastname: Gutiérrez
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques; D008543:Melanins; D009154:Mutation; D009183:Mycotoxins / Q000032:analysis*; D012680:Sensitivity and Specificity; D016808:Tetrahymena thermophila / Q000235:genetics
chemical_list: D008543:Melanins; D009183:Mycotoxins; C028063:melanin precursors