title: Steroidal saponins from Asparagus officinalis and their cytotoxic activity.
abstract: Two oligofurostanosides were isolated from the seeds of Asparagus officinalis L and their structures characterized as 3-O-[alpha-L-rhamnopyranosyl-(1-->2)-(alpha-L-rhamnopyranosyl- (1-->4))-beta-D-glucopyranosyl]-26-O-[beta-D-glucopyranosyl]-(25R) -22 alpha-methoxyfurost-5-ene-3 beta,26-diol(methyl protodioscin) and its corresponding 22 alpha-hydroxy analogue (protodioscin). The structural identification was performed using detailed analysis of 1H- and 13C-NMR spectra including two-dimensional NMR spectroscopy (COSY, HMQC, NOESY and HMBC), and chemical conversions. These two compounds have been shown to inhibit the growth of human leukemia HL-60 cells in culture and macromolecular synthesis in a dose-dependent manner. The inhibitory effect on DNA synthesis was found to be irreversible.
authors.affiliation: Department of Plant Science, Cook College, Rutgers, State University of New Jersey, New Brunswick 08903, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Shao
authors.forename: O
authors.initials: O
authors.lastname: Poobrasert
authors.forename: E J
authors.initials: EJ
authors.lastname: Kennelly
authors.forename: C K
authors.initials: CK
authors.lastname: Chin
authors.forename: C T
authors.initials: CT
authors.lastname: Ho
authors.forename: M T
authors.initials: MT
authors.lastname: Huang
authors.forename: S A
authors.initials: SA
authors.lastname: Garrison
authors.forename: G A
authors.initials: GA
authors.lastname: Cordell
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D010936:Plant Extracts; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D012639:Seeds / Q000737:chemistry; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D014675:Vegetables / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012503:Saponins; D013256:Steroids