title: Sensitive high-performance liquid chromatographic assay for aminoglycosides in biological matrices enables the direct estimation of bacterial drug uptake.
abstract: Following the development of a sensitive high-performance liquid chromatographic (HPLC) assay for gentamicin in biological matrices, the utility of this assay for the determination of other clinically important aminoglycosides (neomycin, netilmicin and sisomicin) in bacterial culture media or plasma is demonstrated. The high sensitivity of the assay enables direct measurement of the aminoglycoside content of bacterial cells cultured in the presence of unlabelled drug.
authors.affiliation: School of Pharmacy, The Robert Gordon University, Schoolhill, Aberdeen, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Stead
authors.forename: R M
authors.initials: RM
authors.lastname: Richards
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D013293:Enterococcus faecalis / Q000378:metabolism*; D006801:Humans; D009355:Neomycin / Q000032:analysis* / Q000097:blood; D009428:Netilmicin / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012853:Sisomicin / Q000032:analysis* / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D009428:Netilmicin; D009355:Neomycin; D012853:Sisomicin