title: [The study of the relationship between the granule size of immunoliposomes and the conjugate ratio of F(ab')2 of McAb HI30 (CD45)].
abstract: The immunoliposomes were prepared by conjugating the F(ab')2 of monoclonal antibodies HI30(CD45) labelled by 125I with the liposomes labelled by 131I, and then were separated by a sephacryl S-300 column. The chromatography showed that the liposomes prepared by ultrasonic method were not homogeneous, and the liposomes of smaller granule size had a higher conjugate ratio. It was also found that the fragment F(ab')2 of McAb conjugated to liposomes could enlarge their granule size.
authors.affiliation: Institute of Hematology, CAMS, Tianjin.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: D
authors.initials: D
authors.lastname: Shen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008081:Liposomes; D010316:Particle Size; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D008081:Liposomes; D017493:Leukocyte Common Antigens