Does poly(ADP-ribosyl)ation regulate the DNA methylation pattern?
The existence of a possible correlation between poly(ADP-ribosyl)ation and DNA methylation processes was investigated. In vivo and in vitro experiments were carried out on L929 mouse fibroblasts preincubated for 24 h with or without 3-aminobenzamide, a well-known inhibitor of poly(ADP-ribose) polymerase. Both experimental approaches evidenced a close relationship between these two important nuclear enzymatic mechanisms, suggesting that the poly(ADP-ribosyl)ated isoform of H1 histone and/or long and branched protein-free ADP-ribose polymers could act as protecting agents against full methylation of the CpG dinucleotides in genomic DNA.
Zardo
G
G
Department of Biomedical Sciences and Technologies, University of L'Aquila, I-67100 L'Aquila, Italy.
D'Erme
M
M
Reale
A
A
Strom
R
R
Perilli
M
M
Caiafa
P
P
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D019175:DNA Methylation; D005347:Fibroblasts / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D051379:Mice; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism
D006657:Histones; D011064:Poly Adenosine Diphosphate Ribose; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases