title: Susceptibility to apoptosis is enhanced in immature cortical neurons.
abstract: The susceptibility of cortical neurons to two forms of apoptotic death was compared with susceptibility to excitotoxic death during development in vitro (DIV 4-21). Murine cortical cultures were exposed for 48 h to the phosphatase inhibitor cyclosporine, the protein kinase inhibitor staurosporine or the excitotoxin N-methyl-D-aspartate (NMDA). Susceptibility to apoptosis induced by staurosporine or cyclosporine was maximal between DIV 4-10 and declined from DIV 10 through 18. The opposite pattern was observed with susceptibility to NMDA receptor-mediated excitotoxic necrosis, which was minimal at DIV 6 and progressively increased through DIV 21.
authors.affiliation: Center for the Study of Nervous System Injury and Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: McDonald
authors.forename: M I
authors.initials: MI
authors.lastname: Behrens
authors.forename: C
authors.initials: C
authors.lastname: Chung
authors.forename: T
authors.initials: T
authors.lastname: Bhattacharyya
authors.forename: D W
authors.initials: DW
authors.lastname: Choi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured / Q000502:physiology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000254:growth & development; D016572:Cyclosporine / Q000494:pharmacology*; D051379:Mice; D016202:N-Methylaspartate / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*
chemical_list: D016202:N-Methylaspartate; D016572:Cyclosporine