Versatile insertion plasmids for targeted genome manipulations in bacteria: isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli O157:H7 genome.
A system of versatile insertion plasmids was constructed that permits efficient delivery of the target sites of an ultra-rare-cutting endonuclease and the recombinase FLP into preselected sites of the bacterial genome. With the help of this system, the pathogenicity island LEE of the Escherichia coli O157:H7 genome was excised and isolated in vitro, deleted in vivo, rescued as a plasmid, and transferred into another strain.
Pósfai
G
G
Institute of Biochemistry, Biological Research Center, Szeged, Hungary. posfaigy@everx.szbk.u-szeged.hu
Koob
M D
MD
Kirkpatrick
H A
HA
Blattner
F R
FR
D001483:Base Sequence; D004269:DNA, Bacterial; D019453:Escherichia coli O157 / Q000235:genetics* / Q000472:pathogenicity; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids; D017384:Sequence Deletion
D004269:DNA, Bacterial