title: Borrelia burgdorferi uridine kinase: an enzyme of the pyrimidine salvage pathway for endogenous use of nucleotides.
abstract: The 621 bp udk gene encoding Borrelia burgdorferi potential uridine kinase, involved in the pyrimidine salvage pathway, was cloned and sequenced. The B burgdorferi protein has a molecular mass of 24 kDa in sodium dodecyl sulfate-polyacrylamide gel. The N-terminal sequence of the protein, Ala-Lys-Ile-Ile, is identical to that predicted but lacks N-terminal methionine. udk is located at around 15 kb from the left telomere and forms an operon with an upstream ORF. A likely hypothesis for the role of the pyrimidine salvage pathway is the sole use of endogenous nucleotides for Borrelia.
authors.affiliation: Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Boursaux-Eude
authors.forename: D
authors.initials: D
authors.lastname: Margarita
authors.forename: A M
authors.initials: AM
authors.lastname: Gilles
authors.forename: O
authors.initials: O
authors.lastname: Bârzu
authors.forename: I
authors.initials: I
authors.lastname: Saint Girons
mesh_terms: D000595:Amino Acid Sequence; D015748:Borrelia burgdorferi Group / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009876:Operon; D011743:Pyrimidines / Q000378:metabolism*; D014541:Uridine Kinase / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
chemical_list: D011743:Pyrimidines; D014541:Uridine Kinase