title: Incidence of aneuploid spermatozoa among infertile men studied by multicolor fluorescence in situ hybridization.
abstract: We studied by fluorescence in situ hybridization the frequency of aneuploidy in spermatozoa of 12 infertile men: 8 with normal or nearly normal semen analysis values and 4 with oligo-astheno-teratozoospermia. The control group consisted of 18 normal healthy fertile men. Probes for chromosome 1 and 7 were used and 10,000 spermatozoa per individual were scored. The hybridization efficiency was good (higher than 98%). In the group with nearly normal semen analysis values the frequencies of spermatozoa disomic for chromosome 1 or chromosome 7 were 0.08% and 0.07%, respectively, and not elevated compared to controls (0.10% and 0.06%, respectively). The frequency of diploid spermatozoa was 0.17%, not significantly different from the control group (0.15%) either. In the group of oligoastheno-teratozoospermic men both the frequencies of disomic cells for chromosome 1 (0.22%) and for chromosome 7 (0.13%) and of diploid spermatozoa (0.56%) were significantly higher compared to controls, although this was mainly due to one patient with high frequencies of hyperploid sperm. The results indicate that infertility may be a risk factor for chromosomal aneuploidy in spermatozoa.
authors.affiliation: Department of Medical Genetics and Center for Reproductive and Developmental Medicine, University of Turku, Finland. jaana.lahdetie@utu.fi
authors.forename: J
authors.initials: J
authors.lastname: Lähdetie
authors.forename: N
authors.initials: N
authors.lastname: Saari
authors.forename: M
authors.initials: M
authors.lastname: Ajosenpää-Saari
authors.forename: J
authors.initials: J
authors.lastname: Mykkänen
mesh_terms: D000328:Adult; D000782:Aneuploidy; D002878:Chromosomes, Human, Pair 1; D002897:Chromosomes, Human, Pair 7; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015994:Incidence; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D013094:Spermatozoa