title: Rhinoviruses induce interleukin-8 mRNA and protein production in human monocytes.
abstract: Rhinoviruses are important upper respiratory pathogens that are strongly associated with asthma exacerbations. However, the inflammatory response to rhinovirus infection is poorly understood. Interleukin (IL)-8 has been implicated in the pathogenesis of respiratory viral infections and asthma. Rhinovirus-induced IL-8 release and mRNA induction were examined in peripheral blood mononuclear cells (PBMC). Rhinoviruses induced IL-8 release for up to 7 days after inoculation onto PBMC. This was associated with an increase in IL-8 mRNA expression that peaked 48 h after exposure to the virus. IL-8 protein production was reduced by UV inactivation of the virus and abolished by preventing virus-receptor binding. Although rhinovirus replication was not demonstrated in PBMC, low-grade productive infection was shown in the human monocyte cell line THP-1. Rhinovirus induction of IL-8 in monocytes or airway macrophages may be important in the pathogenesis of rhinovirus-induced asthma exacerbation.
authors.affiliation: Division of Pulmonary, Critical Care and Occupational Medicine, University of Iowa College of Medicine, and VA Medical Center, Iowa City, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Johnston
authors.forename: A
authors.initials: A
authors.lastname: Papi
authors.forename: M M
authors.initials: MM
authors.lastname: Monick
authors.forename: G W
authors.initials: GW
authors.lastname: Hunninghake
mesh_terms: D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000494:pharmacology; D016209:Interleukin-8 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear; D009000:Monocytes / Q000378:metabolism* / Q000821:virology*; D010850:Picornaviridae Infections / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012229:Rhinovirus / Q000187:drug effects / Q000254:growth & development*; D014466:Ultraviolet Rays
chemical_list: D016209:Interleukin-8; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1