Effects of tromethamine buffer on coagulation variables and ionized calcium concentration in dogs.
OBJECTIVE
To evaluate coagulation variables in 2 groups of dogs after tromethamine administration.


METHODS
13 Beagles.


METHODS
Both groups of dogs received a 30-minute IV infusion of 10 ml of 0.3M tromethamine/kg of body weight. In unsedated dogs (group 1, n = 8), prothrombin time, activated partial thromboplastin time, normalized ionized calcium concentration, platelet numbers, and platelet function were measured prior to treatment, at the end of the infusion, and 1 hour after the infusion. In xylazine-sedated dogs (group 2, n = 5), buccal mucosal bleeding time and plasma percentage of von Willebrand factor antigen were measured before and 1 hour after infusion, and fibrin degradation products concentration was measured 1 hour after infusion. Platelet function was assessed by determining platelet aggregation and by measuring ATP release from the aggregating platelets over 6 minutes, using a whole blood aggregometer, with 20, 10, and 5 microM ADP and 5 and 10 micrograms of collagen/ml as platelet activation agonists.


RESULTS
There was no significant change in any of the variables measured in either group of dogs, compared with baseline values.


CONCLUSIONS
When administered to healthy dogs, tromethamine does not change the coagulation indices measured.
Moon
P F
PF
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA.
Barr
S C
SC
Erb
H N
HN
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D001792:Blood Platelets / Q000737:chemistry; D002021:Buffers; D002118:Calcium / Q000097:blood*; D004285:Dogs / Q000378:metabolism*; D005260:Female; D008297:Male; D010314:Partial Thromboplastin Time; D010974:Platelet Aggregation / Q000187:drug effects; D011517:Prothrombin Time; D014325:Tromethamine / Q000494:pharmacology*
D002021:Buffers; D014325:Tromethamine; D002118:Calcium