title: Human Supt5h protein, a putative modulator of chromatin structure, is reversibly phosphorylated in mitosis.
abstract: The Saccharomyces cerevisiae proteins Spt4p, Spt5p and Spt6p are involved in transcriptional repression by modulating the structure of chromatin. From HeLa cells we have purified a human homologue of Spt5p, Supt5hp, and show here that the protein is reversibly phosphorylated in mitosis. The cloned cDNA predicts a protein of 1087 residues with 31% identity to yeast Spt5p. It includes an acidic N-terminus, a putative nuclear localization signal and a C-terminal region containing two different repeated motifs. One of them, with the consensus sequence P-T/S-P-S-P-Q/A-S/G-Y, is similar to the C-terminal domain in the largest subunit of RNA polymerase II.
authors.affiliation: Deutsches Krebsforschungszentrum, Division for Molecular Biology of Mitosis, Heidelberg, Germany.
authors.forename: A A
authors.initials: AA
authors.lastname: Stachora
authors.forename: R E
authors.initials: RE
authors.lastname: Schäfer
authors.forename: M
authors.initials: M
authors.lastname: Pohlmeier
authors.forename: G
authors.initials: G
authors.lastname: Maier
authors.forename: H
authors.initials: H
authors.lastname: Ponstingl
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology; D002843:Chromatin / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D035602:Transcriptional Elongation Factors
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; C069940:SPT5 transcriptional elongation factor