[Reversion of malignant phenotypes of human lung squamous carcinoma cells by ornithine decarboxylase antisense RNA].
Abnormally elevated activity of ornithine decarboxylase (ODC), with subsequent polyamine accumulation are intimately associated with the genesis, development and metastasis of cancer. Therefore, ODC antisense RNA was used to transfect human lung squmous carcinoma cell line LTEP-78. Compared with the parental cells, growth of the antisense transfected LTEP-78 cells arrested in G0/G1 phase and colony formation in soft agar and tumorigenicity in nude mice were significantly reduced. Nucleic acid hybridization demonstrated the expression of ODC antisense RNA and the content of ODC mRNA was markedly reduced. The results suggest that the reversion of malignant phenotypes of human lung squamous carcinoma cells is associated with the control of polyamine biosynthesis.
Guan
J
J
China-Japan Friendship Institute of Clinical Medical Sciences, Beijing.
Fan
M
M
Cao
S
S
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics*; D010641:Phenotype; D016372:RNA, Antisense / Q000096:biosynthesis / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
D016372:RNA, Antisense; D012333:RNA, Messenger; D009955:Ornithine Decarboxylase