Recombinant human granulocyte colony-stimulating factor increases circulating CD34-postive cells in patients with AIDS.
In a gene therapy-based treatment of AIDS, it would be desirable to have as many transduced target cells as possible. A limiting factor is the number of target cells. In this study, we investigated whether it was possible to increase the absolute number of one possible target cell, i.e., the circulating hematopoietic progenitor cells (CD34 cells) in patients with AIDS, using the recombinant human granulocyte colony-stimulating factor (G-CSF). Eight patients with AIDS were treated with G-CSF for neutropenia (< 1.0 x 10(9)/l). Treatment consisted of daily subcutaneous injections with 300 micrograms G-CSF for 3-5 consecutive days. Within 5 days of initiation of G-CSF therapy, an increase in the absolute neutrophil count (ANC) was seen in all patients. There was a median increase in ANC from 0.4 to 3.4 x 10(9)/l. In addition, G-CSF treatment significantly increased the absolute number of CD34 cells. The median increase in CD34 cells was from 0.8 to 2.2 x 10(6)/l. Finally, using a highly sensitive HIV-1 RNA PCR, we found that treatment of AIDS patients with G-CSF did not lead to enhanced HIV replication. These observations indicate that G-CSF may be used to mobilize CD34 cells in patients with AIDS, e.g., for a gene therapy protocol.
Nielsen
S D
SD
Department of Infectious Diseases, Hvidovre Hospital, Denmark.
Dam-Larsen
S
S
Nielsen
C
C
Wantzia
P
P
Mathiesen
L
L
Hansen
J E
JE
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000188:drug therapy*; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D006678:HIV / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007958:Leukocyte Count / Q000187:drug effects; D008297:Male; D009504:Neutrophils; D011994:Recombinant Proteins / Q000627:therapeutic use; D014779:Virus Replication / Q000187:drug effects
D018952:Antigens, CD34; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor