[Specific inhibition of anti-DNA antibody production by an anti-DH ribozyme].
In order to develop a new therapy for systemic lupus erythematosus, we designed a ribozyme (catalytic RNA) to specifically cleave the mRNA of anti-DNA-responsible VH genes and tested for their ability to regulate the production of autoantibody by human B cell clone O-81 expressing nephritogenic idiotypes. Based on a preliminary antisense experiments for O-81 VH gene, we determined the regions in O-81 mRNA, which would be the target site for the cleavege. The O-81 ribozyme specifically inhibited the production of anti-DNA antibody by O-81 cones. For application to clinical state, nuclease-resistance ribozyme has been expected. These results indicate to introduce ribozymes as a new method of therapy in autoimmune diseases.
Suzuki
Y
Y
Second Department of Internal Medicine, Tohoku University School of Medicine.
Funato
T
T
D000974:Antibodies, Antinuclear / Q000096:biosynthesis* / Q000235:genetics; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D005803:Genes, Immunoglobulin; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000628:therapy*; D016337:RNA, Catalytic / Q000627:therapeutic use*; D012333:RNA, Messenger
D000974:Antibodies, Antinuclear; D016337:RNA, Catalytic; D012333:RNA, Messenger