Cationic phosphonolipids as non viral vectors for DNA transfection in hematopoietic cell lines and CD34+ cells.
The ability to transfer genes into a hematopoietic stem cell and to achieve regulation of their expression in lymphoid or myeloid lineages should open many new therapeutic opportunities. Besides gene transfer mediated by virus vectors like retrovirus or adenovirus, non viral systems have the theoretical advantage of being safe and easy to manage. We developed a new family of cationic lipids called phosphonolipids, synthesized 24 new molecules, and then in a first step we tested their potential to transfer genes in human hematopoietic cell lines (K562 and TF1). A LacZ plasmid under the control of a strong viral promoter was used as a reporter gene and a FACS-Gal assay and a quantitative test CPRG assay evaluated the beta gal expression. The targeted cells were analyzed 48 hours after transfection. The present work shows that seven novel molecules display a high transfer efficiency. One of them is nine-fold more efficient than the commercially available cationic lipids. The results obtained ex vivo on CD34 cells with the FACS-Gal assay show that at day 10 after transfection, 45 percent of cells are expressing gal.
Floch
V
V
Centre de Biogénétique, University, Hospital, ETSBO, Brest, France.
Le Bolc'h
G
G
Audrézet
M P
MP
Yaouanc
J J
JJ
Clément
J C
JC
des Abbayes
H
H
Mercier
B
B
Abgrall
J F
JF
Férec
C
C
D018952:Antigens, CD34 / Q000032:analysis*; D002453:Cell Cycle; D002733:Chlorophenols / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D005697:Galactosides / Q000378:metabolism; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D008081:Liposomes; D010743:Phospholipids; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
D018952:Antigens, CD34; D002733:Chlorophenols; D005697:Galactosides; D008081:Liposomes; D010743:Phospholipids; C028299:phosphonolipids; D004247:DNA; C074184:chlorophenol red galactopyranoside