HLA-B27 modulates intracellular survival of Salmonella enteritidis in human monocytic cells.
Human major histocompatibility complex class I allele HLA-B27 is associated with a group of diseases called spondyloarthropathies. In reactive arthritis (ReA), the disease is triggered by certain infections, e.g. gastroenteritis caused by Salmonella. The host/microbe interaction is abnormal in susceptible individuals leading to inefficient elimination of arthritis-triggering bacteria, fragments of them, or both, after the initial infection. Using transfected human monocytic U937 cell lines, we demonstrate that the expression of the HLA-B27 antigen does not influence the uptake of S. enteritidis into U937 cells in vitro. Interestingly, HLA-B27 remarkably impairs the elimination of S. enteritidis within the HLA-B27 transfected U937 cells. The impaired elimination of ReA-triggering microbes by HLA-B27+ monocytes may offer an explanation for the persistence of ReA-triggering microbes in susceptible HLA-B27+ individuals. This modulation of the host/microbe interaction by HLA-B27 may have an important role in the pathogenesis of ReA.
Laitio
P
P
National Public Health Institute, Turku, Finland. pailai@utu.fi
Virtala
M
M
Salmi
M
M
Pelliniemi
L J
LJ
Yu
D T
DT
Granfors
K
K
D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology; D005805:Genes, MHC Class I / Q000276:immunology; D015796:HLA-B27 Antigen / Q000502:physiology*; D006801:Humans; D007424:Intracellular Fluid / Q000276:immunology* / Q000382:microbiology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology* / Q000648:ultrastructure; D010587:Phagocytosis; D000091402:Prohibitins; D012477:Salmonella enteritidis / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D014162:Transfection
D015796:HLA-B27 Antigen; C000717331:PHB2 protein, human; D000091402:Prohibitins