title: Post-SELEX combinatorial optimization of aptamers.
abstract: In vitro selection techniques provide a means of isolating nucleic acid ligands for binding to particular protein targets. Although most aptamers have quite high affinities for their target proteins, it has been shown that post-SELEX modification can result in further enhancement of binding affinity, as well as other desired properties. This has led to the current development of a more systematic approach to aptamer optimization using a combinatorial screening methodology.
authors.affiliation: NeXstar Pharmaceuticals, Inc., Boulder, CO 80301, USA.
authors.forename: B E
authors.initials: BE
authors.lastname: Eaton
authors.forename: L
authors.initials: L
authors.lastname: Gold
authors.forename: B J
authors.initials: BJ
authors.lastname: Hicke
authors.forename: N
authors.initials: N
authors.lastname: Janjić
authors.forename: F M
authors.initials: FM
authors.lastname: Jucker
authors.forename: D P
authors.initials: DP
authors.lastname: Sebesta
authors.forename: T M
authors.initials: TM
authors.lastname: Tarasow
authors.forename: M C
authors.initials: MC
authors.lastname: Willis
authors.forename: D A
authors.initials: DA
authors.lastname: Zichi
mesh_terms: D001483:Base Sequence; D007256:Information Systems; D008024:Ligands; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000138:chemical synthesis / Q000378:metabolism; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship
chemical_list: D008024:Ligands; D009841:Oligonucleotides; D011506:Proteins; D012313:RNA