[The construction of 2 BS cell lines overexpressing PKC beta I isoform and the priliminary analysis of the relationship between expression of PKC beta I and cell proliferation].
Using gene transfection technique, we have constructed a series of human embryonic lung cell lines that overexpress stably a full length cDNA encoding the beta I isoform of PKC for the first time. BS-PKC 3 is a cell line containing about 3 fold greater PKC activity than parental cell lines or control cells that carry an integrated vector lacking the cDNA inset. In comparison with control cells and parental cells, these cells exhibit significantly enhanced growth rate. The expression of oncogene c-myc which is relating closely to cell proliferation is also increased obviously in BS-PKC 3 cell. We have firstly evidence that the overexpression PKC beta I affect the level of the expression of c-myc in the 2 BS cells. It seems that there may be one of the molecular mechanisms of the effect on the proliferation in the 2 BS cells by PKC beta I.
Ling
D H
DH
Department of Biology, Beijing Normal University.
Liu
H T
HT
D002455:Cell Division; D002460:Cell Line; D005347:Fibroblasts / Q000201:enzymology; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008168:Lung / Q000166:cytology; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D007527:Isoenzymes; D011493:Protein Kinase C