title: Distinct mechanisms of splicing regulation in vivo by the Drosophila protein Sex-lethal.
abstract: The protein Sex-lethal (SXL) controls pre-mRNA splicing of two genes involved in Drosophila sex determination: transformer (tra) and the Sxl gene itself. Previous in vitro results indicated that SXL antagonizes the general splicing factor U2AF65 to regulate splicing of tra. In this report, we have used transgenic flies expressing chimeric proteins between SXL and the effector domain of U2AF65 to study the mechanisms of splicing regulation by SXL in vivo. Conferring U2AF activity to SXL relieves its inhibitory activity on tra splicing but not on Sxl splicing. Therefore, antagonizing U2AF65 can explain tra splicing regulation both in vitro and in vivo, but this mechanism cannot explain splicing regulation of Sxl pre-mRNA. These results are a direct proof that Sxl, the master regulatory gene in sex determination, has multiple and separable activities in the regulation of pre-mRNA splicing.
authors.affiliation: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientificas, Velázquez 144, 28006 Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Granadino
authors.forename: L O
authors.initials: LO
authors.lastname: Penalva
authors.forename: M R
authors.initials: MR
authors.lastname: Green
authors.forename: J
authors.initials: J
authors.lastname: Valcárcel
authors.forename: L
authors.initials: L
authors.lastname: Sánchez
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D017344:Genes, Insect; D007301:Insect Hormones / Q000235:genetics; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D007301:Insect Hormones; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C067777:Sxl protein, Drosophila