title: Regional cerebral blood flow measurement with iodine-123-IMP autoradiography: normal values, reproducibility and sensitivity to hypoperfusion.
abstract: We recently proposed a simplified technique for measuring regional cerebral blood flow (rCBF) using the [123I]N-isopropyl-p-iodoamphetamine (IMP) autoradiographic (ARG) method with SPECT (the IMP-ARG method). We examined normal values of rCBF and the reproducibility and sensitivity to hypoperfusion in stroke patients using this method.
  
  
  METHODS
  By using a standard arterial input, a single static scan, a fixed distribution volume (Vd) and one-point arterial blood sampling, we measured rCBF in 39 normal volunteers (19 men and 20 women; mean ages 61 +/- 11 yr for the men and 60 +/- 12 yr for the women). Eighteen neurologically stable patients with prior stroke (mean age = 65 +/- 11 yr) were studied twice at a mean interval of 97 days. In 16 patients (7 men and 9 women, mean age = 63 +/- 5 yr) with subarachnoid hemorrhage, rCBF was measured 1-2 wk after onset. Cerebral vasospasm was evaluated by repeated angiography. The mean rCBF in the vasospastic area was compared with that in a nonvasospastic area.
  
  
  RESULTS
  The mean rCBFs of the cerebral cortex and centrum semiovale in the volunteers were 33.0 +/- 5.1 ml/100 g/min and 25.0 +/- 4.5 ml/100 g/min, respectively. There was no age-dependent change in rCBF, but the women showed significantly higher cortical rCBF than the men (p < 0.05). In the stroke patients, the whole-brain CBF values showed high reproducibility, with high correlations between those obtained at the first and second studies (y = -3.5 + 1.03x; r = 0.90; p < 0.001). In the subarachnoid hemorrhage patients, the vasospastic area showed significantly lower rCBF than the normal cortical rCBF (p < 0.01) and the nonvasospastic area (p < 0.01). Brain regions with rCBF levels below 20 ml/100 g/min showed infarction on the follow-up CT scan.
  
  
  CONCLUSIONS
  The IMP-ARG method is reproducible, sensitive to hypoperfusion and feasible for the quantitative evaluation of rCBF in routine clinical practice.
authors.affiliation: Department of Radiology and Nuclear Medicine, Akita Research Institute of Brain and Blood Vessels, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Hatazawa
authors.forename: H
authors.initials: H
authors.lastname: Iida
authors.forename: E
authors.initials: E
authors.lastname: Shimosegawa
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
mesh_terms: D000368:Aged; D000662:Amphetamines; D001345:Autoradiography; D002544:Cerebral Infarction / Q000000981:diagnostic imaging / Q000503:physiopathology; D002560:Cerebrovascular Circulation; D002561:Cerebrovascular Disorders / Q000000981:diagnostic imaging / Q000503:physiopathology; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D020367:Iofetamine; D008297:Male; D008875:Middle Aged; D011877:Radionuclide Imaging; D012016:Reference Values; D015203:Reproducibility of Results; D013345:Subarachnoid Hemorrhage / Q000000981:diagnostic imaging / Q000503:physiopathology
chemical_list: D000662:Amphetamines; D007457:Iodine Radioisotopes; D020367:Iofetamine