title: Functional and morphological outcome of knee joint transplantation in dogs depends on control of rejection.
abstract: BACKGROUND
  The reconstruction of massive osteochondral defects extending to weight-bearing joints remains a surgical challenge. Total knee joint transplantation has been performed experimentally, but these studies lacked prospective evaluation of functional outcome, graft vascularization, and graft viability.
  
  
  METHODS
  Replantation and transplantation of vascularized knee joints was performed in dogs (n=4 per group), comparing functional and morphological results during a 6-month follow-up.
  
  
  RESULTS
  All replant recipients and three transplant recipients survived the 6-month follow-up period. At this time, duplex sonography and angiography revealed patent anastomoses in all animals. Increases in volumetric flow rates and vascular collateralization were observed in allografts, as compared with replanted joints (100+/-16 ml/min vs. 31+/-15 ml/min at 6 months after transplantation). Bone fusion at the graft-host interface was verified by fluorography in all animals at 3 months after transplantation. Six months after transplantation, microradiographies and computerized tomographies revealed spongialization of the cortical bone and filling of the medullary space by trabecular bone in transplanted joints. Such alterations were not detectable in replanted joints. Chondrocyte viability exceeded 80% in all but one transplanted joint. Lymphocyte infiltration of synovia and arterial walls was detected in all transplanted joints, suggesting the presence of chronic rejection. Weight-bearing capacity recovered in all replanted animals (weight-bearing index before transplantation: 0.499+/-0.080; 6 months after transplantation: 0.38+/-0.16) but only in two of four transplanted animals (weight-bearing index 6 months after transplantation: 0.37, 0.28, and 0.00).
  
  
  CONCLUSIONS
  These data demonstrate the potential of joint grafting and the critical dependence of allotransplantation on the control of rejection.
authors.affiliation: Department of Surgery, University of Basel, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Rosso
authors.forename: D
authors.initials: D
authors.lastname: Schäfer
authors.forename: R
authors.initials: R
authors.lastname: Fricker
authors.forename: J
authors.initials: J
authors.lastname: Brennwald
authors.forename: G
authors.initials: G
authors.lastname: Jundt
authors.forename: G
authors.initials: G
authors.lastname: Spagnoli
authors.forename: F
authors.initials: F
authors.lastname: Harder
authors.forename: M
authors.initials: M
authors.lastname: Heberer
mesh_terms: D000792:Angiography; D000818:Animals; D016572:Cyclosporine / Q000627:therapeutic use; D004285:Dogs; D005260:Female; D005470:Fluorometry; D006084:Graft Rejection / Q000517:prevention & control*; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007596:Joints / Q000637:transplantation*; D007717:Knee / Q000098:blood supply / Q000601:surgery*; D008297:Male; D018919:Neovascularization, Physiologic; D012092:Replantation; D014184:Transplantation, Homologous
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine