High expression of human amelogenin in E. coli.
A human cDNA, encoding for the 175-amino-acid human amelogenin, was prepared by RT PCR from tooth bud mRNA and sub-cloned into pGEX-KG expression plasmid for over-expression in E. coli. The expressed protein was characterized by SDS-PAGE Western blotting, and N-terminal amino acid sequencing.
Deutsch
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D
Department of Oral Biology, Hebrew University Hadassah, Faculty of Dental Medicine, Jerusalem, Israel.
Chityat
E
E
Hekmati
M
M
Palmon
A
A
Farkash
Y
Y
Dafni
L
L
D053523:Amelogenin; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D015153:Blotting, Western; D003001:Cloning, Molecular; D003746:Dental Enamel Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
D053523:Amelogenin; C507200:Amelx protein, mouse; D001426:Bacterial Proteins; D003746:Dental Enamel Proteins; D011993:Recombinant Fusion Proteins