title: Flipper, a mobile Fot1-like transposable element in Botrytis cinerea.
abstract: A transposable element, Flipper, was isolated from the phytopathogenic fungus Botrytis cinerea. The element was identified as an insertion sequence within the coding region of the nitrate reductase gene. The Flipper sequence is 1842 bp long with perfect inverted terminal repeats (ITRs) of 48 bp and an open reading frame (ORF) of 533 amino acids, potentially encoding for a transposase; the element is flanked by the dinucleotide TA. The encoded protein is very similar to the putative transposases of three elements from other phytopathogenic fungi, Fot1 from Fusarium oxysporum, and Pot2 and MGR586 from Magnaporthe grisea. The number of Flipper elements in strains of B. cinerea varied from 0 to 20 copies per genome. Analysis of the descendants of one cross showed that the segregation ratio of Flipper elements was 2:2 and that the copies were not linked.
authors.affiliation: Station de Pathologie Végétale, INRA, Route de Saint Cyr, Versailles,France.
authors.forename: C
authors.initials: C
authors.lastname: Levis
authors.forename: D
authors.initials: D
authors.lastname: Fortini
authors.forename: Y
authors.initials: Y
authors.lastname: Brygoo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D003904:Mitosporic Fungi / Q000235:genetics*; D008969:Molecular Sequence Data; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004251:DNA Transposable Elements; D009565:Nitrate Reductases; D050901:Nitrate Reductase