Evidence for a tissue-specific induction of cutaneous CYP2E1 by dexamethasone.
We studied in mouse the effect of topical application of dexamethasone or salicylic acid, on CYP2E1 and CYP3A expression (proteins and/or mRNA) in liver and skin. Dexamethasone was also administered by intraperitoneal injection. Topical application or intraperitoneal injection of dexamethasone increased cutaneous CYP2E1 (8 and 4-fold respectively) whereas the hepatic level of this isoform showed a slight decrease and hepatic CYP3A expression was increased (3-fold). Cutaneous CYP2E1 was increased (3-fold) after topical treatment by salicylic acid. This compound had no effect on hepatic CYP3A and CYP2E1 expression. Cutaneous CYP3A (protein and mRNA) was not detectable in all groups (control or treated animals). Dexamethasone and salicylic acid increased cutaneous CYP2E1 mRNA level (2.5 and 1.4-fold respectively). In conclusion, dexamethasone and salicylic acid induced cutaneous CYP2E1 protein and mRNA level. Cutaneous CYP2E1 induction by dexamethasone is a tissue-specific process.
Sampol
E
E
UPRES EA 2194, Université de la Méditerranée, Faculté de pharmacie,Marseille, France.
Mirrione
A
A
Villard
P H
PH
Piccerelle
P
P
Scoma
H
H
Berbis
P
P
Barra
Y
Y
Durand
A
A
Lacarelle
B
B
D000287:Administration, Topical; D000818:Animals; D019392:Cytochrome P-450 CYP2E1 / Q000096:biosynthesis*; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D007274:Injections, Intraperitoneal; D051379:Mice; D008815:Mice, Inbred Strains; D008861:Microsomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D051381:Rats; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D012867:Skin / Q000201:enzymology*
D012459:Salicylates; D003907:Dexamethasone; D019392:Cytochrome P-450 CYP2E1; D020156:Salicylic Acid