Detection of potato mop-top virus capsid readthrough protein in virus particles.
Potato mop-top furovirus (PMTV) RNA 3 encodes the 20 kDa coat protein and a larger readthrough protein of 67 kDa. The readthrough protein is expressed by suppression of the amber stop codon which terminates the coat protein gene. A 21 kDa C-terminal fragment of the readthrough protein was doned, fused to glutathione S-transferase and expressed in E. coli. An antiserum prepared against purified fusion protein was used in ELISA to detect the readthrough protein in extracts of PMTV-infected leaves. Immunogold labelling studies showed that the readthrough protein was located near one extremity of some of the virus particles.
Cowan
G H
GH
Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Torrance
L
L
Reavy
B
B
D000818:Animals; D000914:Antibodies, Viral; D002213:Capsid / Q000032:analysis* / Q000235:genetics; D010942:Plant Viruses / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D012328:RNA Viruses / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D011198:Solanum tuberosum / Q000821:virology; D014771:Virion / Q000737:chemistry
D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins