title: Consequences of a subtle sialic acid modification on the murine polyomavirus receptor.
abstract: Polyomaviruses are small, nonenveloped DNA tumor viruses with restricted host ranges. Virus binding to cell surface receptors is one determinant of viral tropism. Although murine polyomavirus is among the best characterized viruses, little is known about the sialic acid-containing receptor and its interaction with viral particles. By using nonradioactive virus binding assays as recently described for the B-lymphotropic papovavirus, murine polyomavirus particles were found to bind in a saturable and noncooperative manner to 25,000 receptors per 3T6 mouse fibroblast. The virus-receptor interaction at 4 degrees C was of high affinity (Kd = 1.8 x 10(-11) M), very fast (k1 = 1.7 x 10(7) M(-1) s(-1)), and stable (half-life = 38 min). Elongation of the N-acyl side chain of sialic acid by biosynthetic modulation with synthetic precursor analogs has been shown for other polyomaviruses to influence both sialic acid-dependent binding and infection (O. T. Keppler, P. Stehling, M. Herrmann, H. Kayser, D. Grunow, W. Reutter, and M. Pawlita, J. Biol. Chem. 270:1308-1314, 1995). In 3T6 cells in which about one-third of the sialic acids were modified, infection and binding of polyomavirus particles were significantly reduced. The number of receptors per cell was decreased to 18,000, with the remaining receptors displaying the same affinity as in untreated cells. Molecular modeling studies based on the three-dimensional structure of a mouse polyomavirus-sialyllactose complex recently solved by T. Stehle and coworkers (T. Stehle, Y. W. Yan, T. L. Benjamin, and S. C. Harrison, Nature 369:160-163, 1994) were performed. They suggest that the elongation of the N-acyl side chain by a single methylene group leads to steric hinderence, with the peptide backbone of a loop walling the tip of the shallow sialic acid binding groove. This collision appears to be incompatible with functional binding. The data are taken as a basis to discuss possible features of the organization and topology of the cellular receptor for mouse polyomavirus.
authors.affiliation: Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Herrmann
authors.forename: C W
authors.initials: CW
authors.lastname: von der Lieth
authors.forename: P
authors.initials: P
authors.lastname: Stehling
authors.forename: W
authors.initials: W
authors.lastname: Reutter
authors.forename: M
authors.initials: M
authors.lastname: Pawlita
mesh_terms: D000818:Animals; D051379:Mice; D008958:Models, Molecular; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D011120:Polyomavirus / Q000502:physiology*; D011991:Receptors, Virus / Q000737:chemistry* / Q000502:physiology; D013329:Structure-Activity Relationship
chemical_list: D011991:Receptors, Virus; D019158:N-Acetylneuraminic Acid