[Secretion of endothelin-1 by cultured human normal adrenal cells].
Endothelin (ET) is a vasoactive peptide produced by some other types of cells in addition to endothelial cells. The authors investigated into the possibility of ET-1 secretion by cultured human normal adrenal cells. Human normal adrenal cells were prepared by 2% collagenase digestion for 1.5 hours and cultured in DMEM supplemented with 10% FCS. Angiotensin I (10(-9)-10(-7) mol/L) was added to the experimental groups on the 7-9th days of cultivation. Mediums were collected after 24 hours and the levels of aldosterone, cortisol and ET-1 in the mediums were measured by RIA. Other than aldosterone and cortisol, ET-1 was detected in the cultured mediums of human normal adrenal cells. And the secretion of ET-1 was stimulated by angiotesin I. Therefore, it is proposed that ET-1 may play a role by autocrine or paracrine in the human normal adrenal gland.
Pu
Y
Y
PUMC Hospital, CAMS, Beijing.
Zeng
Z
Z
Sun
M
M
Guan
B
B
Li
H
H
D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D000450:Aldosterone / Q000378:metabolism; D000804:Angiotensin II / Q000494:pharmacology; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000378:metabolism*; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D008297:Male; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D019332:Endothelin-1; D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D000450:Aldosterone; D006854:Hydrocortisone