title: Humoral response of dairy cattle to spirochetes isolated from papillomatous digital dermatitis lesions.
abstract: OBJECTIVE
  To determine whether a humoral response against spirochetes isolated from papillomatous digital dermatitis (PDD) lesions is elicited in dairy cattle affected with PDD.
  
  
  METHODS
  41 cattle with PDD from 8 dairies (study population) and 30 cattle from 2 dairies free of PDD (control population). Additionally evaluated were 32 cattle from a dairy with a past history of PDD but no current disease, and 52 cattle from a dairy with high prevalence of PDD, 25 with and 27 without detectable lesions.
  
  
  METHODS
  ELISA were used to evaluate the humoral response of all cattle to representative isolates from 2 groups of spirochetes of unknown species isolated from PDD lesions. Specificity of the response was evaluated, using immune sera prepared against each of the spirochetes, and by adsorption studies of immune and field sera. The potential for confounding by an antibody response to other spirochetes associated with diseases of cattle was assessed.
  
  
  RESULTS
  The antibody response (specific) to both PDD spirochete groups of cows with PDD was significantly increased, compared with that of cows from PDD-free dairies. There was no association between antibody response to PDD-associated spirochetes and antibody response to other spirochetal diseases of cattle. None of the cattle from the dairy with previous history of PDD but without current disease were classified as test positive by either PDD ELISA. There was a significant (P < 0.01) difference in classification results for both PDD ELISA for cattle with PDD from the dairy with a high herd prevalence of PDD, compared with cattle without detectable disease from the same dairy.
  
  
  CONCLUSIONS
  The humoral response in cattle with PDD lesions was significantly different from that in cattle without detectable lesions, thus providing additional information regarding the potential role of spirochetes isolated from PDD lesions in the etiopathogenesis of PDD.
authors.affiliation: California Veterinary Diagnostic Laboratory System, University of California, Davis 95616, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Walker
authors.forename: D H
authors.initials: DH
authors.lastname: Read
authors.forename: K J
authors.initials: KJ
authors.lastname: Loretz
authors.forename: D W
authors.initials: DW
authors.lastname: Hird
authors.forename: S L
authors.initials: SL
authors.lastname: Berry
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002417:Cattle / Q000276:immunology; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D003612:Dairying; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005533:Foot Dermatoses / Q000382:microbiology / Q000662:veterinary*; D013144:Spirochaetales / Q000276:immunology / Q000302:isolation & purification*; D013145:Spirochaetales Infections / Q000276:immunology / Q000662:veterinary*