The role of insulin-like growth factor-I (IGF-I) and estradiol in rabbit corpus luteum progesterone production.
To determine whether insulin-like growth factor-I (IGF-I) plays a role in rabbit corpus luteum (CL) physiology the authors examined: IGF-I expression, the effect of IGF-I on progesterone (P) production in vitro, and the interaction of IGF-I with estradiol, the primary luteotropin in the rabbit. Northern blot analysis revealed that IGF-I mRNA is present in the rabbit CL throughout pseudopregnanacy. An intact ovarian in vitro perfusion model and dispersed luteal cell culture were used to determine effects of IGF-I on P production and interactions with estradiol. IGF-I significantly stimulated P production compared to control medium by the isolated, intact perfused rabbit ovary and by dispersed, cultured luteal cells. Estradiol alone did not stimulate P production in vitro. Estradiol did augment IGF-I stimulated P production in the intact perfused ovary and in luteal cell culture. These findings support a role for IGF-I in rabbit CL P production.
Chen
S H
SH
Department of Gynecology and Obstetrics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Zanagnolo
V
V
Preutthipan
S
S
Roberts
K P
KP
Goodman
S B
SB
Dharmarajan
A M
AM
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism*; D004958:Estradiol / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000502:physiology*; D010053:Ovary / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*; D011555:Pseudopregnancy / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D011863:Radioimmunoassay
D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I