Heme metabolism in promastigotes of Leishmania donovani.
Promastigotes of Leishmania donovani (Dd-8 strain) showed presence of important key enzymes of heme synthesizing (delta-aminolevulinic acid synthase and ferrochelatase) and degrading (heme oxygenase and biliverdin reductase) systems, classical leishmanicidal drugs viz allopurinol, amphotericin B, pentamidine and CDRI compound 93/202 inhibited the heme oxygenase activity of the parasite, whereas, delta-aminolevulinic acid synthase activity practically remained unaffected. The Km, Vmax and pH values of heme oxygenase of promastigotes were found to be 1666 microM hemin, 625 nmol of bilirubin formed h-1 mg protein-1 and 7.5 respectively. The findings suggest the presence and importance of heme metabolism in the de novo synthesis of different hemoproteins of the Leishmania parasite as well as the detoxification and its defence against biological insults.
Srivastava
P
P
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Sharma
G D
GD
Kamboj
K K
KK
Rastogi
A K
AK
Pandey
V C
VC
D000624:5-Aminolevulinate Synthetase / Q000032:analysis*; D000818:Animals; D005294:Ferrochelatase / Q000032:analysis*; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007893:Leishmania donovani / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D015800:Protozoan Proteins / Q000032:analysis*
D015800:Protozoan Proteins; D010088:Oxidoreductases; D006419:Heme Oxygenase (Decyclizing); D044925:Oxidoreductases Acting on CH-CH Group Donors; C019789:biliverdin reductase; D000624:5-Aminolevulinate Synthetase; D005294:Ferrochelatase