Expression of recombinant rat interleukin-13 (IL-13) and generation of a neutralizing rat IL-13 antiserum.
Using baculoviral and bacterial systems, we expressed biologically active recombinant rat IL-13 and generated neutralizing rat IL-13 antiserum. Recombinant rat IL-13 produced by baculovirus-infected insect cells stimulated proliferation of TF-1 premyeloid cell line and induced expression of 15-lipoxygenase mRNA in human peripheral blood monocytes. Antiserum generated by immunizing a rabbit with recombinant bacterial rat IL-13 specifically inhibited TF-1 proliferation induced by baculoviral rat IL-13 but did not neutralize human IL-13 mitogenic activity. Western blotting with anti-rat IL-13 serum revealed a approximately 12 kD protein band in supernatants of insect cells infected with recombinant baculovirus carrying the rat IL-13 cDNA. The availability of recombinant rat IL-13 and rat IL-13 antibodies should facilitate studying the role of IL-13 in rat models of human inflammatory disorders.
Lakkis
F G
FG
Renal Division, Emory University School of Medicine and Veterans Affairs Medical Center, Atlanta, Georgia 30033, USA. fglakki@emory.edu
Cruet
E N
EN
Nassar
G M
GM
Badr
K F
KF
Pascual
D W
DW
D000818:Animals; D000906:Antibodies; D016367:Baculoviridae; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D007249:Inflammation; D018793:Interleukin-13 / Q000096:biosynthesis* / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008970:Molecular Weight; D009500:Neutralization Tests; D011817:Rabbits; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D018411:Spodoptera; D014162:Transfection
D000906:Antibodies; D018793:Interleukin-13; D011994:Recombinant Proteins