High-performance liquid chromatographic analysis of Peptide T in rabbit plasma with on-line column enrichment.
The present paper describes the development of a simple and sensitive analytical method for quantification of Peptide T (PT) in rabbit plasma, using standard analytical equipment and on-line column enrichment, without prior extraction, clean-up or derivatization. The analytical procedure was found to be accurate, precise and linear. The accuracy was 100% (range 97-103%) and the mean precision was 8% (range 3-14%) for all (n=6) concentrations (0, 15, 50, 100 and 200 ng/ml). The total recovery was found to be approximately 80%, and it was found to be dependent upon the injection rate onto the extraction column. The correlation between added and found concentrations was 0.9982, and the limit of detection was estimated to be around 5 ng/ml. The method is therefore found to be suitable for bioavailability studies, involving Peptide T, in rabbits.
Bechgaard
E
E
The Royal Danish School of Pharmacy, Department of Pharmaceutics, Copenhagen, Denmark.
Bagger
M
M
Larsen
R
R
Nielsen
H W
HW
D000281:Administration, Intranasal; D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D007275:Injections, Intravenous; D015717:Peptide T / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D011817:Rabbits; D013050:Spectrometry, Fluorescence
D015717:Peptide T