title: Generation of H2O2 by human neutrophils and changes of cytosolic Ca2+ and pH of rat thymocytes in response to galactoside-binding proteins (lectins or immunoglobulins).
abstract: In contrast to plant agglutinins, biological activities of animal/human lectins are not well defined yet. Testing a panel of seven mammalian carbohydrate-binding proteins we have found that the dimeric lectin from chicken liver (CL-16) was a stimulator of H2O2 release from human neutrophils as well as effector for induction of cytosolic Ca2+ and pH increase in rat thymocytes. Activity of this lectin was comparable to potent galactoside-specific plant lectins such as Viscum album L. agglutinin. The activities of the tested plant lectins depended significantly on their nominal carbohydrate specificity as well as on the source. The results indicate that endogenous lectins may be involved in the regulation of neutrophil and lymphocyte functions by elicitation of selective biosignaling reactions.
authors.affiliation: Department of Biophysics, Belarusian State University, Minsk, Belarus.
authors.forename: A V
authors.initials: AV
authors.lastname: Timoshenko
authors.forename: S
authors.initials: S
authors.lastname: André
authors.forename: H
authors.initials: H
authors.lastname: Kaltner
authors.forename: X
authors.initials: X
authors.lastname: Dong
authors.forename: H J
authors.initials: HJ
authors.lastname: Gabius
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002645:Chickens; D003600:Cytosol / Q000737:chemistry; D004357:Drug Synergism; D037161:Galectins; D006001:Glycoconjugates / Q000494:pharmacology; D006388:Hemagglutinins / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007136:Immunoglobulins / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism*; D037121:Plant Lectins; D010944:Plants; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D037161:Galectins; D006001:Glycoconjugates; D006388:Hemagglutinins; D007136:Immunoglobulins; D037102:Lectins; D037121:Plant Lectins; D000584:Amiloride; D006861:Hydrogen Peroxide; D002118:Calcium