title: The conserved HR domain of the Drosophila suppressor 2 of zeste [Su(z)2] and murine bmi-1 proteins constitutes a locus-specific chromosome binding domain.
abstract: The related Drosophila Suppressor 2 of zeste [Su(z)2] and Posterior sex combs (Psc) proteins are both locus-specific chromosome binding proteins. They are found at many of the same polytene chromosome loci as other Polycomb-group proteins. The 1,365 amino acid Su(z)2 protein and the 1,603 amino acid Psc protein share a conserved 200 amino acid domain, the homology region (HR). To identify the protein domain responsible for locus-specific chromosome binding, we made a series of Hsp70:cDNA deletion constructs of the Sz(z)2 gene and transformed these into flies. We found that the HR is necessary and sufficient for Su(z)2 locus-specific polytene chromosome binding. The murine Bmi-1 protein also shares the conserved HR domain. When expressed in flies, the Bmi-1 protein showed a locus-specific chromosome binding pattern similar to that of the Su(z)2 and Psc proteins. These results argue that a locus-specific chromosome binding function resides in the HR domain. Other results show that a second, low affinity, non-specific chromosome binding function is localized outside the HR in the Su(z)2 protein, and that the Su(z)2 protein contains at least two nuclear localization signals.
authors.affiliation: Biology Department and Cancer Center, University of Virginia, Charlottesville, VA 22903, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Sharp
authors.forename: N A
authors.initials: NA
authors.lastname: Abramova
authors.forename: W J
authors.initials: WJ
authors.lastname: Park
authors.forename: P N
authors.initials: PN
authors.lastname: Adler
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D000013:Congenital Abnormalities; D004268:DNA-Binding Proteins; D004330:Drosophila / Q000033:anatomy & histology / Q000235:genetics; D029721:Drosophila Proteins; D016259:Genes, myc; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D019076:Transgenes; D014960:X Chromosome / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C071051:Su(z)2 protein, Drosophila