Alterations in brain tumor DNA detected by a fingerprinting probe.
We used a novel DNA fingerprinting probe O-chi-1 (ref. 1) to detect differences in the hybridization pattern of brain tumor DNA and paired normal tissue of a given individual. Representatives of meningiomas (two), glioblastoma multeforme (three) and astrocytoma (one) were studied. Alterations, which included amplification as well as the loss of a normal band in tumor DNA, were observed in four of the six tumours. While the increased intensity of a band can be taken to imply increased copy number, the disappearance of bands could either be due to loss of DNA sequence or rearrangement resulting in different sized bands.
Joshi
A R
AR
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
Sinha
S
S
Dil-Afroz
Sulaiman
I M
IM
Banerji
A K
AK
Hasnain
S E
SE
D001254:Astrocytoma / Q000235:genetics; D015139:Blotting, Southern; D001932:Brain Neoplasms / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005909:Glioblastoma / Q000235:genetics; D006801:Humans; D008579:Meningioma / Q000235:genetics
D015342:DNA Probes; D004273:DNA, Neoplasm; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases