A low energy short hydrogen bond in very high resolution structures of protein receptor--phosphate complexes.
A very short hydrogen bond between an Asp and a phosphate is established in two high resolution structures (0.98 and 1.05 A). A mutant complex that changes the Asp to an Asn, which forms a normal hydrogen bond, has a similar free energy of binding to the wild type complex, suggesting that the contribution of the short hydrogen bond is not extraordinarily strong.
Wang
Z
Z
Luecke
H
H
Yao
N
N
Quiocho
F A
FA
D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D009154:Mutation; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation
D002352:Carrier Proteins; D028044:Phosphate-Binding Proteins; D010710:Phosphates