title: Degradation behaviour of a new bioceramic: Ca2P2O7 with addition of Na4P2O7.10H2O.
abstract: A newly produced bioceramic, beta-Ca2P2O7 with addition of Na4P2O7.10H2O (SDCP), has been implanted into the femoral condyle of rabbits. Within 6 weeks after implantation, most of the bioceramic is replaced by new woven bone. On the contrary, block from hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP), which are osteoconductible, do not resorb within a short period of time. We believe that the biodegradable behaviour of SDCP may occur in two steps. The first and most important step is the digestion of particles and migration of the particles by phagocytosis. The object of this study is to examine the change in morphologies, chemical compositions and crystal structure of SDCP after soaking in distilled water for a certain period of time. The SDCP ceramic was also co-cultured with leucocytes to observe how the SDCP particles were digested by the leucocytes, so that the mechanism of biodegradable behaviour of SDCP ceramic in vivo might be clarified. Four types of sintered calcium phosphate ceramics were tested in the experiment: SDCP, pure beta-Ca2P2O7 (DCP), HA and beta-TCP. They wee soaked in distilled water at 37 degrees C for up to 30 days. The microstructure and morphology of crystals deposited on the surface were observed using scanning electron microscopy. Sodium, calcium and phosphorus ion contents in the supernatant solution were detected by atomic absorption analysis and ion coupled plasma. In summary, HA and DCP showed no significant evidence of dissolution in distilled water. In static distilled water, calcium ions may be released from beta-TCP into solution during the initial 7 days and then converted into HA by reprecipitation. The results showed that the SDCP was firstly dissolved into small grains or fragments by the solution. The small fragments should be so small as to be digested by the phagocytes in a physiological environment.
authors.affiliation: Centre for Biomedical Engineering, College of Medicine, National Taiwan University, Taipei, ROC.
authors.forename: F H
authors.initials: FH
authors.lastname: Lin
authors.forename: C J
authors.initials: CJ
authors.lastname: Liao
authors.forename: K S
authors.initials: KS
authors.lastname: Chen
authors.forename: J S
authors.initials: JS
authors.lastname: Sun
authors.forename: H C
authors.initials: HC
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000493:pharmacokinetics*; D001673:Biodegradation, Environmental; D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D002516:Ceramics / Q000493:pharmacokinetics*; D011756:Diphosphates / Q000138:chemical synthesis / Q000493:pharmacokinetics*; D007700:Kinetics; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis; D010710:Phosphates / Q000032:analysis; D011817:Rabbits; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011756:Diphosphates; D010710:Phosphates; C107241:diphosphoric acid; D002118:Calcium