Nonexponential kinetics of a single tRNAPhe molecule under physiological conditions.
The fluorescence decay functions of individual, specifically labeled tRNAPhe molecules exhibit nonexponential character as a result of conformational dynamics occurring during the measurement on a single molecule. tRNAPhe conformational states that interchange much more slowly are evidenced by the distribution of lifetimes observed for many individual molecules. A structural model for the nonexponential decay indicates that the tRNAPhe-probe adduct fluctuates between two states, one of which provides conditions that quench the probe fluorescence.
Jia
Y
Y
Chemistry Department, University of Pennsylvania, Philadelphia, PA 19104, USA.
Sytnik
A
A
Li
L
L
Vladimirov
S
S
Cooperman
B S
BS
Hochstrasser
R M
RM
D003198:Computer Simulation; D005453:Fluorescence; D007700:Kinetics; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D012360:RNA, Transfer, Phe / Q000737:chemistry / Q000378:metabolism*
D012360:RNA, Transfer, Phe