Recombinant histamine-releasing factor enhances IgE-dependent IL-4 and IL-13 secretion by human basophils.
Human recombinant histamine-releasing factor (HrHRF) is known to directly stimulate histamine release and IL-4 secretion from basophils of selected atopic donors in a reaction requiring the expression of a particular type of IgE, referred to as IgE+. In this study, HrHRF is shown to affect the IgE-mediated release of IL-4, IL-13, and histamine from basophils not normally releasing to this protein (i.e, those expressing IgE-). Priming with several different concentrations of HrHRF for 15 min enhanced basophil secretion of IL-4 and histamine after 4 h in a dose-dependent fashion following activation with anti-IgE Ab (10 ng/ml). This effect of HrHRF priming also occurred in cultures activated with 1 or 100 ng/ml of anti-IgE Ab. The secretion of IL-13 protein was enhanced similarly by HrHRF priming in cultures stimulated for 16 to 20 h with anti-IgE Ab. There were, however, no apparent changes in the secretion of histamine or cytokine by basophils primed with HrHRF and activated with the IgE-independent secretogogue, FMLP. These findings suggest that HrHRF modifies the response of basophils for IgE-dependent secretion by binding to a specific receptor, broadening the possible role of this protein in chronic allergic inflammation.
Schroeder
J T
JT
Johns Hopkins University, Department of Medicine, Baltimore, MD 21224, USA.
Lichtenstein
L M
LM
MacDonald
S M
SM
D001491:Basophils / Q000276:immunology / Q000378:metabolism*; D014408:Biomarkers, Tumor; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D018793:Interleukin-13 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D008222:Lymphokines / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D018793:Interleukin-13; D008222:Lymphokines; D011994:Recombinant Proteins; D000090324:Tumor Protein, Translationally-Controlled 1; D015847:Interleukin-4; D007073:Immunoglobulin E