Development of enzyme immunoassay of 2'-deoxycytidine.
In order to study 2'-deoxycytidine (2'-dCyd) as a possible prognostic marker in cancer chemotherapy, an enzyme immunoassay (EIA) was developed. 2'-dCyd as a hapten was succinylated and two omicron-monosuccinyl-2'-dCyd's were purified by high performance liquid chromatography and identified by mass spectrometry and 1H-NMR. Two antigens were prepared by conjugating two omicron-succinyl derivatives with keyhole limpet hemocyanin (KLH) as a carrier. Both antigen produced specific antibodies to 2'dCyd in BALD/c mice. The spleen cells of one mouse immunized with 5'-omicron-succinyl-2'-dCyd-KLH were hybridized with myeloma cells. One monoclone selected produced a specific antibody. A convenient EIA was attained by using the monoclonal antibody.
Omura
K
K
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Hirose
K
K
Itoh
M
M
Akizawa
T
T
Yoshioka
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D003429:Cross Reactions; D003841:Deoxycytidine / Q000032:analysis*; D007124:Immunoenzyme Techniques; D051379:Mice
D000911:Antibodies, Monoclonal; D000941:Antigens; D003841:Deoxycytidine