title: 5 alpha reductase activity in human gingiva and gingival fibroblasts in response to bacterial culture supernatants, using [14C]4-androstenedione as substrate.
abstract: 5 alpha-Reduction of androgen substrates is increased in inflamed gingiva. It was therefore relevant to study the effect of bacterial culture supernatants derived from Prevotella intermedius (P.i), Porphyromonas gingivalis (P.g) and Actinobacillus actinomycetemcomitans (A.a) on the metabolism of [14C]4-androstenedione to 5 alpha-dihydrotestosterone (DHT) in gingival tissue and cultured fibroblasts. Chronically inflamed human gingival tissue and cultured gingival fibroblasts from the same source were incubated in duplicate with [14C]4-androstenedione and optimal concentrations of P.i, P.g. and A.a culture supernatants in Eagle's minimal essential medium in a CO2 incubator for 24 h at 37 degrees C. The metabolites were then extracted, separated and quantified using a radioisotope scanner. There were 87, 50 and 6% increases in DHT synthesis by human gingival tissue in response to the culture supernatants of P.i, P.g and A.a, respectively, over control incubations (n = 3; p < 0.01: Wilcoxon signed-ranked statistic for paired observations). With the cells in culture, all four fibroblast cell lines produced DHT and testosterone from [14C]4-androstenedione in varying amounts. The production of DHT was enhanced in the presence of each the bacterial culture supernatants to varying degrees (P.i 40%, P.g 35% and A.a 40%; p < 0.01). Combinations of the bacterial extracts: (P.i + P.g), (P.i + A.a), (P.g + A.a) and (P.i + P.g + A.a) showed intermediate or suppressor effects on DHT formed compared with individual incubations. Culture supernatants of these pathogens can influence DHT synthesis in fibroblasts, and effect that is modulated by baseline androgen metabolism and the proportion of virulent pathogens present. This may have some bearing on host susceptibility on host and the progression of the periodontal lesion.
authors.affiliation: Department of Periodontology, King' College School of Medicine and Dentistry, London, UK.
authors.forename: M
authors.initials: M
authors.lastname: Soory
authors.forename: S
authors.initials: S
authors.lastname: Ahmad
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000472:pathogenicity; D000735:Androstenedione / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000472:pathogenicity*; D002478:Cells, Cultured; D042944:Cholestenone 5 alpha-Reductase; D017077:Culture Media, Conditioned / Q000378:metabolism; D003773:Dental Plaque / Q000382:microbiology; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000378:metabolism; D005891:Gingivitis / Q000378:metabolism* / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010088:Oxidoreductases / Q000378:metabolism*; D010514:Periodontal Pocket / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000472:pathogenicity; D018713:Prevotella intermedia / Q000472:pathogenicity
chemical_list: D017077:Culture Media, Conditioned; D013196:Dihydrotestosterone; D000735:Androstenedione; D010088:Oxidoreductases; D042944:Cholestenone 5 alpha-Reductase