In vivo fluorescence kinetics of porphyrins following intravesical instillation of 5-aminolaevulinic acid in normal and tumour-bearing rat bladders.
The fluorescence kinetics of protoporphyrin IX (PPIX) following intravesical instillation of 5-aminolaevulinic acid (5-ALA) have been studied in vivo in a rat bladder tumour model. 5-ALA dissolved in NaHCO3 was intravesically instilled for 60 min in tumour-bearing and normal bladders of Wistar rats. The fluorescence was excited with the violet lines of a Kr(+)-laser and recorded in vivo by means of a fibre coupled optical multichannel analyser. The fluorescence emission bands of PPIX at lambda = 636 nm and lambda = 708 nm were detected in normal and tumorous urothelium after only 30 min. The maximum fluorescence intensity was obtained in tumorous and normal urothelium 3-4 h after instillation. The ratio of the fluorescence intensity in tumorous to normal urothelium decreased continuously from four to about two during the time range of 6 h. PPIX fluorescence following 5-ALA instillation could also be observed in kidney and liver. Fluorescence from further porphyrin species with emission bands at lambda = 617 nm and lambda = 682 nm was detected in the bladder, indicating an efflux of hydrophilic porphyrins from the hepatic pathway.
Heil
P
P
LFL-Laser-Forschungslabor, Urologische Klinik der Ludwig-Maximilians-Universität München, Germany.
Stocker
S
S
Sroka
R
R
Baumgartner
R
R
D000283:Administration, Intravesical; D000622:Aminolevulinic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D005260:Female; D007700:Kinetics; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D011524:Protoporphyrins / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*
D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX