Simple approach for analysis of plasma oxo-, hydroxy- and dicarboxylic acids.
Following deproteinization of plasma with organic solvents the supernatant was shaken with hexane and cation-exchange resin in an Eppendorf tube to remove fatty and amino acids and the medium was subjected to direct treatment with ethyl chloroformate under catalytic influence of pyridine. A subsequent extraction of the immediately formed ethyl esters with a drop of chloroform enabled us to subject the sample to gas chromatographic (GC) analysis. Since ketocarboxylic acids do not require a preliminary oximation the total time of sample workup and analysis takes only several minutes.
Husek
P
P
Institute of Endocrinology, Prague, Czech Republic.
Matucha
P
P
D002849:Chromatography, Gas; D003998:Dicarboxylic Acids / Q000097:blood*; D005563:Formic Acid Esters; D006801:Humans; D006880:Hydroxy Acids / Q000097:blood*; D007651:Keto Acids / Q000097:blood*; D015203:Reproducibility of Results
D003998:Dicarboxylic Acids; D005563:Formic Acid Esters; D006880:Hydroxy Acids; D007651:Keto Acids; C007658:ethyl chloroformate