title: CHO transfectants produce large amounts of recombinant protein in suspension culture.
abstract: Chinese hamster ovary (CHO) cells transfected with various genes are widely used as adherent cell monolayers to produce recombinant proteins. In this report we present a new culture technique for CHO cells transfected with the vector pPOL-DHFR-CD14 using a minifermenter (miniPERM, Heraeus) for the production of recombinant human endotoxin receptor CD14 (rCD14). The transfectants were cultured for 12-17 days under serum-free conditions and formed spheroids. From this system we harvested supernatants containing up to 3.1 mg/ml recombinant CD14 (rCD14). This represents a 200-fold increase of rCD14 yield compared to conventional adherent CHO cell culture.
authors.affiliation: Institute of Immunology and Transfusion Medicine, Ernst-Moritz-Arndt-University of Greifswald, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Schütt
authors.forename: B
authors.initials: B
authors.lastname: Fürll
authors.forename: F
authors.initials: F
authors.lastname: Stelter
authors.forename: R S
authors.initials: RS
authors.lastname: Jack
authors.forename: S
authors.initials: S
authors.lastname: Witt
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D018929:Cell Culture Techniques; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
chemical_list: D018950:Lipopolysaccharide Receptors; D011994:Recombinant Proteins