title: Concomitant rotavirus serotypes 1 and 4 infections in a diarrhoeic child from Belém, Brazil.
abstract: Concomitant serotypes 1 and 4 infections were detected in a 15-month old female child with community-acquired diarrhoea which lasted 7 days and coursed with moderate dehydration. The evidence for dual rotavirus infection was offered by the following findings: a) enzyme-linked immunosorbent assay (ELISA) positive reactions to both 1 and 4 serotypes; and b) extra-migrating bands at electrophoresis of RNA in polyacrylamide gel (PAGE). These results suggest that children living under poor sanitation conditions are heavily exposed to rotavirus infections; in addition, the co-circulation of different serotypes in the same setting sustains the current concept that a rotavirus vaccine should be multivalent, in order to protect children against the four epidemiologically important rotavirus G serotypes.
authors.affiliation: Serviço de Virologia Geral, Instituto Evandro Changas, Belém, Pará, Brasil.
authors.forename: J D
authors.initials: JD
authors.lastname: Mascarenhas
authors.forename: R H
authors.initials: RH
authors.lastname: Gusmão
authors.forename: Y B
authors.initials: YB
authors.lastname: Gabbay
authors.forename: T A
authors.initials: TA
authors.lastname: Monteiro
authors.forename: J B
authors.initials: JB
authors.lastname: Gomes
authors.forename: A C
authors.initials: AC
authors.lastname: Linhares
mesh_terms: D001938:Brazil; D003968:Diarrhea, Infantile / Q000276:immunology / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D012401:Rotavirus / Q000145:classification*; D012400:Rotavirus Infections / Q000276:immunology*; D012703:Serotyping