Chromosomal translocations deregulating c-myc are associated with normal immune responses.
Plasmacytomas induced in BALB/c mice by pristane consistently evidence chromosomal translocations involving the c-myc gene and one of the Ig loci. This observation has lead to the suggestion that c-myc deregulation is a critical event in the generation of such tumors. However, it is not clear whether c-myc translocation is related to pristane treatment or occurs in normal lymphocyte populations nor whether such translocations occur normally, and at similar frequencies, in strains genetically resistant to plasmacytoma development, such as DBA/2. In order to address these questions, a Long Distance PCR assay with single copy sensitivity was employed to assess the frequency of c-myc/IgA translocations in normal and immunized mice of both plasmacytoma resistant and susceptible lineages in the absence of pristane treatment. Our data demonstrate that spontaneous translocations occur in normal DBA/2 and BALB/c mice with no significant differences in frequency. A 3-5-fold increase in translocation frequency was observed in mice immunized with cholera toxin, a strong stimulator of IgA responses. We conclude that c-myc deregulation by chromosomal translocation is associated with normal physiological processes of B-cell differentiation and, as such, can not be the determining factor leading to malignancy.
Roschke
V
V
Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Kopantzev
E
E
Dertzbaugh
M
M
Rudikoff
S
S
D000818:Animals; D001483:Base Sequence; D002772:Cholera Toxin / Q000276:immunology; D005260:Female; D016259:Genes, myc; D020022:Genetic Predisposition to Disease; D007114:Immunization; D007070:Immunoglobulin A / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D010954:Plasmacytoma / Q000235:genetics* / Q000276:immunology*; D014178:Translocation, Genetic
D007070:Immunoglobulin A; D002772:Cholera Toxin