title: Sequence polymorphism and structural analysis of timothy grass pollen profilin allergen (Phl p 11).
abstract: Three cDNA clones encoding timothy grass pollen profilin (Phl p 11) were newly isolated. Comparison of the sequences of four cDNA clones, including a previously isolated clone, showed a low level of polymorphism. Isoelectrofocusing of highly purified timothy grass profilin indicated the existence of at least five isoforms. One recombinant profilin showed similar immunological properties to natural timothy grass profilin. Tertiary structure of Phleum pratense profilin was obtained by homology-based molecular modeling.
authors.affiliation: R&D Department, IFIDESA-ARISTEGUI, Bilbao, Spain. im000001@sarenet.es
authors.forename: J A
authors.initials: JA
authors.lastname: Asturias
authors.forename: M C
authors.initials: MC
authors.lastname: Arilla
authors.forename: B
authors.initials: B
authors.lastname: Bartolomé
authors.forename: J
authors.initials: J
authors.lastname: Martínez
authors.forename: A
authors.initials: A
authors.lastname: Martínez
authors.forename: R
authors.initials: R
authors.lastname: Palacios
mesh_terms: D000485:Allergens / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003285:Contractile Proteins; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011058:Pollen / Q000235:genetics*; D011110:Polymorphism, Genetic; D051304:Profilins; D017434:Protein Structure, Tertiary
chemical_list: D000485:Allergens; D003285:Contractile Proteins; D018076:DNA, Complementary; D008840:Microfilament Proteins; C084984:PHLPII protein, Phleum pratense; D010940:Plant Proteins; D051304:Profilins