Sequence variation in the hpd gene of nonencapsulated Haemophilus influenzae isolated from patients with chronic bronchitis.
The molecular diversity of protein D of nonencapsulated Haemophilus influenzae strains isolated from persistently infected patients with chronic bronchitis was studied by sequencing the hpd gene of four independently obtained isolates. The nucleotide (nt) sequences of the hpd genes of two strains were identical. The other two hpd sequences showed nt substitutions which were mostly synonymous. As a consequence the deduced amino acid (aa) sequences differed from the consensus sequence only by a few aa. No changes in the hpd genes were observed among the four variants of the four strains persisting in chronic bronchitis patients for 9, 11, 8 and 3 months, respectively, although variation in their major outer membrane proteins P2 and P5 occurred. We conclude that the hpd gene is conserved during chronic infections of nonencapsulated H. influenzae.
Duim
B
B
Department of Medical Microbiology, University of Amsterdam, Academic Medical Center, The Netherlands.
Ruiter
P
P
Bowler
L D
LD
Dankert
J
J
van Alphen
L
L
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D001991:Bronchitis / Q000382:microbiology*; D002352:Carrier Proteins / Q000235:genetics*; D002908:Chronic Disease; D018076:DNA, Complementary; D005798:Genes, Bacterial; D014644:Genetic Variation; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007072:Immunoglobulin D; D008074:Lipoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D007072:Immunoglobulin D; D008074:Lipoproteins; C067989:glpQ protein, Haemophilus influenzae