[The physicochemical characteristics of ribosomal fractions from Pseudomonas aeruginosa obtained by precipitation with polyethylene glycol 6000].
Ribosomal fractions containing up to 72% of ribosomal material and 25% of sugars (among them, about 6% of hexose) were isolated from P.aeruginosa cells, immunotypes F-1, 2, 6 and 7, by precipitation with polyethylene glycol 6000. Lipopolysaccharide, determined in the test for ketodesoxyoctanoic acid, was not detected in these fractions, but, as determined in the passive hemagglutination test, the content of O-antigen in the preparations was 3-25%. O-antigen and ribosome present in the fractions formed a complex, disintegrating after treatment with trilon B.
Egorova
T P
TP
Belkin
Z P
ZP
Nartikova
V F
VF
Avetisian
G L
GL
Sazykina
S Iu
SIu
Fedosova
V G
VG
D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D006385:Hemagglutination Inhibition Tests; D007202:Indicators and Reagents; D019081:O Antigens / Q000032:analysis / Q000302:isolation & purification; D011092:Polyethylene Glycols; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000276:immunology; D012270:Ribosomes / Q000737:chemistry* / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D019081:O Antigens; D011092:Polyethylene Glycols