title: Nonenzymatic antioxidant activity of human seminal plasma.
abstract: OBJECTIVE
  To examine the nonenzymatic antioxidant properties of human seminal plasma.
  
  
  METHODS
  Determination of total (peroxyl) radical-trapping antioxidant parameter and malondialdehyde concentration in seminal plasma of 112 men attending IVF-ET or intracytoplasmic sperm injection (ICSI) programs.
  
  
  METHODS
  University-based center for reproductive medicine.
  
  
  METHODS
  Normozoospermic men, ages 25 to 50 years, of fertility proved by a pregnancy resulting from IVF-ET, and men ages 25 to 50 years with negative IVF-ET or ICSI outcome, distinguished by their smoking status.
  
  
  METHODS
  Seminal plasma was obtained from ejaculates collected for IVF-ET or ICSI procedures.
  
  
  METHODS
  Total (peroxyl) radical-trapping antioxidant parameter and malondialdehyde concentrations.
  
  
  RESULTS
  No significant differences for total (peroxyl)-radical-trapping antioxidant parameter were observed between normozoospermic IVF-ET or asthenozoospermic ICSI patients; neither was a difference revealed for pregnancy-positive or -negative couples within those groups. Age, smoking status, or increased leukocyte count had no effect on total (peroxyl) radical-trapping antioxidant parameter or malondialdehyde concentrations in seminal plasma.
  
  
  CONCLUSIONS
  Nonenzymatic antioxidant activity in seminal plasma does not reflect sperm fecundity ability.
authors.affiliation: Department of Gynecology, Białystok School of Medicine, Poland. MRJOZWIK@OBGYN.EDU.PL
authors.forename: M
authors.initials: M
authors.lastname: Jóźwik
authors.forename: M
authors.initials: M
authors.lastname: Jóźwik
authors.forename: W
authors.initials: W
authors.lastname: Kuczyński
authors.forename: M
authors.initials: M
authors.lastname: Szamatowicz
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D010545:Peroxides / Q000378:metabolism; D011247:Pregnancy; D018873:Pregnancy Rate; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012661:Semen / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D010545:Peroxides; D017382:Reactive Oxygen Species; D008315:Malondialdehyde