Keratin immobilized on silica as a new stationary phase for chromatographic modelling of skin permeation.
Skin permeability of organic compounds depends on their lipophilicity but can also be affected by compounds interactions with specific skin components. A good chromatographic model of percutaneous penetration determined solely by lipophilicity is provided by the immobilized artificial membrane (IAM) columns. To complete the model a new high-performance liquid chromatographic (HPLC) stationary phase was prepared by physical immobilization of keratin on silica support. The keratin immobilized on silica has properties typical for the reversed-phase materials but it retains specifically acidic solutes. The keratin column can be used to conveniently compare keratolytic properties of xenobiotics. It was demonstrated that retention parameters determined on a keratin column can be combined with the retention parameters determined on the IAM column to predict differences in skin permeability within a class of drugs. It has been postulated that HPLC can model skin permeation thus reducing research time and costs as well as the use of laboratory animals.
Turowski
M
M
Medical University of Gdańsk, Department of Biopharmaceutics and Pharmacodynamics, Poland.
Kaliszan
R
R
D002851:Chromatography, High Pressure Liquid; D004817:Epidermis / Q000378:metabolism*; D007633:Keratins; D008055:Lipids; D016015:Logistic Models; D008567:Membranes, Artificial; D008954:Models, Biological; D010539:Permeability; D010636:Phenols / Q000493:pharmacokinetics; D012822:Silicon Dioxide; D012995:Solubility; D013256:Steroids / Q000493:pharmacokinetics; D013329:Structure-Activity Relationship
D008055:Lipids; D008567:Membranes, Artificial; D010636:Phenols; D013256:Steroids; D007633:Keratins; D012822:Silicon Dioxide