Comparison of a monoclonal antibody-based IgM capture ELISA with a neutralization assay for assessing response to trivalent oral poliovirus vaccine.
Monoclone-based IgM capture ELISAs were developed for each of the three poliovirus serotypes and compared with a neutralization assay for detecting response to trivalent oral poliovirus vaccine among 224 infants. The IgM-based response rates were significantly higher than the neutralizing antibody-based rates: 95% versus 83% to poliovirus type 1, 99% versus 94% to poliovirus type 2, and 89% versus 59% to poliovirus type 3. IgM responses to the first vaccine dose were significantly associated between serotypes, suggesting that some of the discordance may reflect a heterotypic IgM response. When the response rates in 4 vaccine formulation groups were compared, group differences using the two assays were similar for poliovirus types 1 and 2 but not for type 3. Therefore, IgM results using these assays may not be adequate substitutes for neutralizing antibody results when determining vaccine response.
Gary
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Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Freeman
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Peñaranda
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Maher
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Anderson
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Pallansch
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MA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M; D007223:Infant; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011051:Poliomyelitis / Q000276:immunology*; D017955:Poliovirus / Q000187:drug effects / Q000276:immunology*; D011055:Poliovirus Vaccine, Oral / Q000276:immunology* / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D011055:Poliovirus Vaccine, Oral