Reconstitution of bile acid transport in a heterologous cell by cotransfection of transporters for bile acid uptake and efflux.
The rat liver canalicular bile acid transporter/ecto-ATPase/cell CAM 105 (CBATP) is a 110-kDa transmembrane phosphoglycoprotein that is thought to have bile acid efflux, ecto-ATPase, and cell adhesion properties. Its extracellular amino-terminal domain is highly homologous to carcinoembryonic antigen (CEA), a glycophosphatidyl inositol-anchored membrane protein with cell adhesion properties and a marker for adenocarcinoma. In the current study, we examined the possibility of more clearly defining the role of CBATP in bile acid efflux by cotransfecting a heterologous cell, the COS cell, with cDNAs for a bile acid importer, the ileal bile acid transporter (IBAT), as well as for CBATP. The results show that when IBAT mediates uptake of [3H]taurocholate to a level 20-fold higher than that achieved previously by nonspecific pinocytosis, CBATP mediates time-, temperature- and concentration-dependent efflux. Efflux of [3H]taurocholate mediated by CBATP in the cotransfected COS cells is saturable and has curvilinear kinetic characteristics (Vmax = 400 pmol/mg protein/min, Km = 70 microM). It is inhibited by 4,4'-diisothiocyanostilbene-2,2-disulfonic acid and dependent on ATP but not dependent on membrane potential. Although CEA could not mediate bile acid efflux in COS cells cotransfected with IBAT and CEA, efflux of [3H]taurocholate was detected in COS cells cotransfected with IBAT and a chimeric molecule having the carboxyl-terminal tail and membrane spanning domain of CBATP and the amino-terminal extracellular tail of CEA. Taken together, these data provide further evidence that CBATP confers bile acid efflux properties on heterologous cells and that its cytoplasmic tail and membrane spanning segment are integral to this property. The data also establish a model system for more clearly defining the molecular determinants of bile acid transport mediated by this molecule.
Sippel
C J
CJ
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Dawson
P A
PA
Shen
T
T
Perlmutter
D H
DH
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D001648:Bile Canaliculi / Q000378:metabolism*; D001692:Biological Transport; D019556:COS Cells; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis; D002352:Carrier Proteins / Q000096:biosynthesis / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary; D006913:Hydroxysteroid Dehydrogenases; D007082:Ileum / Q000378:metabolism; D007700:Kinetics; D008562:Membrane Glycoproteins; D016297:Mutagenesis, Site-Directed; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D013656:Taurocholic Acid / Q000378:metabolism; D014162:Transfection; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
D000251:Adenosine Triphosphatases; D001647:Bile Acids and Salts; D002272:Carcinoembryonic Antigen; D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D006913:Hydroxysteroid Dehydrogenases; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D013656:Taurocholic Acid; C037643:bile acid binding proteins; C435496:AKR1C2 protein, human; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); C027201:ectoATPase