Endothelin receptors and their cellular signal transduction mechanism in human cultured prostatic smooth muscle cells.
1. Endothelin (ET) receptors, and their cellular signal transduction mechanism, were characterized in a primary culture of human prostatic smooth muscle cells (HP cell). 2. [125I]-ET-1 and [125I]-ET-3 binding studies revealed that both ETA and ETB receptors were present in the HP cells, and the ratio of ETA to ETB receptors was 1.4:1. 3. Analysis of ET receptor mRNA by reverse transcription-polymerase chain reaction also demonstrated that HP cells express both ETA and ETB receptors. 4. ET-1 and ET-3 increased intracellular free Ca2+ concentration ([Ca2+]i) in the HP cells in a concentration-dependent manner. Use of subtype selective antagonists BQ-123 and BQ-788, indicated that both ETA and ETB receptors were coupled to an increase in [Ca2+]i. 5. Pretreatment of the cells with pertussis toxin resulted in a significant but partial attenuation of the [Ca2+]i increase mediated through the ETA and ETB receptors. However, sensitivity to pertussis toxin (PTX) was significantly different between them. 6. In conclusion, HP cells possess ETA and ETB receptors. Further, these two endothelin receptor subtypes evoke an increase in [Ca2+]i possibly via the action of different GTP-binding proteins.
Saita
Y
Y
Drug Serendipity Research Laboratories, Yamanouchi Pharmaceutical Co. Ltd., Ibaraki, Japan.
Koizumi
T
T
Yazawa
H
H
Morita
T
T
Takenaka
T
T
Honda
K
K
D002118:Calcium / Q000378:metabolism*; D018929:Cell Culture Techniques; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000494:pharmacology; D006801:Humans; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000494:pharmacology; D037342:Pertussis Toxin; D010880:Piperidines / Q000494:pharmacology; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017466:Receptors, Endothelin / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D016232:Endothelins; D009842:Oligopeptides; D010456:Peptides, Cyclic; D010880:Piperidines; D012333:RNA, Messenger; D017466:Receptors, Endothelin; D010566:Virulence Factors, Bordetella; C086539:BQ 788; D037342:Pertussis Toxin; C072247:cyclo(Trp-Asp-Pro-Val-Leu); D002118:Calcium