Copper-zinc superoxide dismutase and isolectin B4 binding are markers for associative and transhemispheric diaschisis induced by focal ischemia in rat cortex.
Copper/zinc-superoxide dismutase (Cu/Zn-SOD) belongs to a class of enzymes, identified as essential and highly effective endogenous scavengers of cytotoxic oxygen radicals. These radicals contribute to postlesional neurotoxicity. In order to determine the superoxide-scavenging potential of regions affected by unilateral cortical photothrombosis, we studied the changes in the distribution of Cu/Zn-SOD and the appearance of activated microglia by immunohistochemistry and isolectin B4 binding. Four hours postlesion, Cu/Zn-SOD increased significantly within a homotopic area of the contralateral hemisphere and in ipsilateral thalamic nuclei, whereas isolectin B4-positive microglia were upregulated at days 5 and 7 postlesion within the same regions. The contralateral increase in the amount of the superoxide-scavenging Cu/Zn-SOD indicates that this enzyme is induced by a retrograde reaction carried through callosal connections.
Bidmon
H J
HJ
Department of Neuroanatomy, Heinrich-Heine-University, Düsseldorf, Germany.
Oermann
E
E
Schleicher
A
A
Kato
K
K
Kinscherf
R
R
Buchkremer-Ratzmann
I
I
Witte
O W
OW
Zilles
K
K
D000818:Animals; D015415:Biomarkers; D002545:Brain Ischemia / Q000473:pathology / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology*; D002560:Cerebrovascular Circulation / Q000502:physiology; D016166:Free Radical Scavengers / Q000378:metabolism; D007839:Functional Laterality / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism*; D013927:Thrombosis / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology
D015415:Biomarkers; D016166:Free Radical Scavengers; D037102:Lectins; D009249:NADP; D013482:Superoxide Dismutase