A novel method for induction and detection of anaphylactic reaction using the mouse abdominal wall (AW method).
We found that an antigen-specific anaphylaxis was induced by antigen challenge to the abdominal wall, ear auricle, or subcutaneous tissue in mice sensitized 9 days previously with antigen and adjuvant. The anaphylactic reaction was detected by vascular permeability at the injected site 7 minutes after challenge, which was the best time for estimation. A novel method (AW method) for induction and detection of the anaphylactic reaction in mice was established using the abdominal wall as the challenge site. This method could detect the anaphylactic response in mice 1 to 3 weeks after sensitization. The increase in vascular permeability was completely inhibited by administration of diphenhydramine.
Kataoka
H
H
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
Tsuda
A
A
Tsuda
Y
Y
Baba
A
A
Yoshida
H
H
Hirasawa
R
R
Tobimatsu
Y
Y
Nishiguchi
M
M
Semma
M
M
Ito
Y
Y
D000009:Abdominal Muscles / Q000098:blood supply / Q000276:immunology*; D000818:Animals; D000941:Antigens / Q000276:immunology*; D002199:Capillary Permeability / Q000276:immunology; D004396:Coloring Agents; D005070:Evans Blue; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009113:Muramidase / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology*; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000276:immunology
D000941:Antigens; D004396:Coloring Agents; D012965:Sodium Chloride; D005070:Evans Blue; D010047:Ovalbumin; D009113:Muramidase