title: S-adenosylmethionine decarboxylase activity is decreased in the rat cortex after traumatic brain injury.
abstract: S-Adenosyl-L-methionine decarboxylase (SAMdc) and L-ornithine decarboxylase (ODC) are major enzymes regulating polyamine synthesis. Following ischemia, putrescine content increases as a result of posttraumatic activation of ODC and inhibition of SAMdc. These alterations are thought to mediate edema and cell death. The purpose of this study was to quantify SAMdc activity and edema in the brain following controlled cortical impact injury. Anesthetized adult male rats underwent a right parietal craniectomy and were subjected to cortical impact injury. Tissues were obtained from three bilateral regions: parietal cortex, motor area (CPm); parietal cortex, somatosensory area (CPs); and the pyriform cortex (CPF). SAMdc activity was determined in the postmitochondrial fraction from homogenates of fresh, unfrozen tissues by measuring the decarboxylation of S-adenosyl-L-[carboxyl-14C]methionine. Basal SAMdc activity was determined in unoperated rats, and regional differences were noted: Activity was lower in the CPF than in the CPm and CPs. SAMdc activity decreased to the greatest extent in the ipsilateral CPm (impact site) from 1 to 72 h following traumatic brain injury. Significant edema was found in the ipsilateral CPm 1, 8, 16, 24, and 48 h after injury. Decreased SAMdc activity impairs the conversion of putrescine to polyamines and may contribute to delayed pathological changes in the brain after traumatic injury.
authors.affiliation: Bobby R. Alford Department of Otorhinolaryngology and Communicative Sciences, Baylor College of Medicine, Houston, Texas 77030, U.S.A.
authors.forename: C M
authors.initials: CM
authors.lastname: Henley
authors.forename: K
authors.initials: K
authors.lastname: Wey
authors.forename: A
authors.initials: A
authors.lastname: Takashima
authors.forename: C
authors.initials: C
authors.lastname: Mills
authors.forename: E
authors.initials: E
authors.lastname: Granmayeh
authors.forename: I
authors.initials: I
authors.lastname: Krishnappa
authors.forename: C S
authors.initials: CS
authors.lastname: Robertson
mesh_terms: D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism*; D000818:Animals; D001929:Brain Edema / Q000378:metabolism; D001930:Brain Injuries / Q000378:metabolism*; D002540:Cerebral Cortex / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014947:Wounds and Injuries / Q000378:metabolism
chemical_list: D012437:Adenosylmethionine Decarboxylase