title: Lactate production by swine adipocytes: effects of age, nutritional status, glucose concentration, and insulin.
abstract: To develop an alternative model in which to study the relationship between adipose tissue lactate production, obesity, and non-insulin-dependent diabetes mellitus (NIDDM), we investigated lactate production by swine adipocytes. Subcutaneous adipocytes from fasted 3-wk-old, fasted 7-mo-old, and fed 7-mo-old Yucatan minIature swine were isolated and incubated with 0.2, 1, 5, 10, or 25 mM glucose +/- 1 mU/ml insulin. Total glucose metabolism (TGM) was estimated by product summation. Results showed that 1) TGM was threefold greater in cells from fasted 7-mo- vs. 3-wk-old swine (P < 0.05), 2) TGM was 2.7-fold greater in cells from fed 7-mo-old vs. fasted 7-mo-old swine (P < 0.05), 3) insulin failed to stimulate TGM in adipocytes from swine of either age and either nutritional status, and 4) lactate and pyruvate accounted for 34 and 30% of TGM, respectively, in adipocytes from swine of both ages. Similarities in glucose metabolism and lactate production in adipocytes from swine and obese NIDDM humans make the swine a potentially valuable model for studying lactate production associated with obesity and NIDDM.
authors.affiliation: Department of Animal and Nutritional Sciences, University of New Hampshire, Durham 03824, USA.
authors.forename: B K
authors.initials: BK
authors.lastname: Heckler
authors.forename: G B
authors.initials: GB
authors.lastname: Carey
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001706:Biopsy; D002478:Cells, Cultured; D004435:Eating; D005215:Fasting; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D007328:Insulin / Q000494:pharmacology; D007773:Lactates / Q000378:metabolism*; D009752:Nutritional Status / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature
chemical_list: D007328:Insulin; D007773:Lactates; D005947:Glucose