Riboprinting: a tool for the study of genetic diversity in microorganisms.
Classical morphology-based methods of taxonomic and phylogenetic analysis are inadequate in many groups of structurally simple eukaryotes. Molecular methods can generate data independently of the complexity of the organisms' morphology. Riboprinting is one such technique, and involves restriction enzyme analysis of polymerase chain reaction amplified small subunit ribosomal RNA genes. The utility of the method is illustrated with examples from several genera of intestinal and bloodstream parasites. Among the applications of riboprinting are the detection of cryptic genetic variation within species, organism misidentifications and culture mix-ups, independent verification of DNA sequences, and the rapid generation of data useful in phylogenetic analyses.
Clark
C G
CG
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, England, Great Britain. g.clark@lshtm.ac.uk
D000818:Animals; D016844:Blastocystis / Q000145:classification / Q000235:genetics; D016054:DNA, Protozoan / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D004747:Entamoeba / Q000145:classification / Q000235:genetics; D056890:Eukaryota / Q000145:classification* / Q000235:genetics; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D015203:Reproducibility of Results; D015183:Restriction Mapping; D014351:Trypanosomatina / Q000145:classification / Q000235:genetics
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012335:RNA, Ribosomal