Full-term development after transfer of nuclei from 4-cell and compacted morula stage embryos to enucleated oocytes in the mouse.
The developmental ability of enucleated mouse oocytes reconstituted between the nucleus from 4-cell mouse embryos at different cell stages and recipient cytoplasms at different conditions, and the developmental ability of oocytes receiving nuclei from compacted morulae were examined. The highest development was observed with the nucleus at the G1 stage fused with cytoplasm at the M stage. Although the enucleated oocytes receiving a nucleus from 4-cell embryos did not develop after transfer to a recipient female, young were obtained after renuclear transfer to enucleated fertilized eggs. Live mice were also obtained from the nucleus of compacted morula.
Tsunoda
Y
Y
Laboratory of Animal Reproduction, College of Agriculture and Research Institute for Animal Developmental Biotechnology, Kinki University, Nara, Japan.
Kato
Y
Y
D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002459:Cell Fusion / Q000502:physiology; D002467:Cell Nucleus / Q000502:physiology; D046508:Culture Techniques; D003593:Cytoplasm / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009028:Morula / Q000502:physiology*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy