Molecular cloning and characterization of a cDNA encoding the rat interleukin-8 receptor.
In this study we present the cloning, characterization and expression analysis of a cDNA encoding a rat interleukin-8 receptor (rIL-8R). A 1324 bp cDNA containing an open reading of 359 amino acids with an 86.1% overall identity with the previously characterized mouse IL-8R was isolated. Genomic DNA analysis using several restriction enzymes revealed a single band suggesting that the rIL-8R gene exists as a single-copy, which is in contrast to humans where there are two different IL-8Rs genes. Expression of rIL-8R mRNA was found in several tissues including spleen, heart, lung, liver, skeletal muscle and kidney. In brain and testis rIL-8R mRNA was not detectable. Rat IL-8R mRNA expression at the cellular level was studied in the spleen using RNA-RNA in situ hybridization and immunohistochemistry. IL-8R mRNA containing cells were predominately found in the mantle zone of the germinal center. These cells were identified as B lymphocytes using the OX-33 monoclonal antibody.
Gobl
A E
AE
Department of Internal Medicine, University Hospital, Uppsala, Sweden. anders.gobl@medicin.uu.se
Huang
M R
MR
Wang
S
S
Zhou
Y
Y
Oberg
K
K
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D018628:Gene Dosage; D005786:Gene Expression Regulation / Q000235:genetics; D018858:Germinal Center / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008214:Lymphocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D018123:Receptors, Interleukin / Q000737:chemistry / Q000235:genetics*; D023062:Receptors, Interleukin-8A; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism
D015703:Antigens, CD; D018076:DNA, Complementary; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A