Analysis of creatinine, vanilmandelic acid, homovanillic acid and uric acid in urine by micellar electrokinetic chromatography.
A simultaneous determination of vanilmandelic acid, homovanillic acid, creatinine and uric acid using capillary electrophoresis was investigated. The optimum conditions of buffer concentration, pH and surfactant concentration were studied, and high resolution was obtained using a 30 mM phosphate buffer (pH 7.0) containing 150 mM sodium dodecyl sulfate. The detection was by UV absorbance at 245 nm and the column was a fused-silica capillary of 67 cm x 75 microm I.D.. The determination of these metabolites in human urine was completed within 15 min without any interferences.
Shirao
M K
MK
Department of Pharmacology, The Institute of Public Health, Shirokanedai Minato-ku, Tokyo, Japan.
Suzuki
S
S
Kobayashi
J
J
Nakazawa
H
H
Mochizuki
E
E
D002021:Buffers; D003404:Creatinine / Q000652:urine*; D019075:Electrophoresis, Capillary; D006719:Homovanillic Acid / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents; D014527:Uric Acid / Q000652:urine*; D014642:Vanilmandelic Acid / Q000652:urine*
D002021:Buffers; D013501:Surface-Active Agents; D014527:Uric Acid; D012967:Sodium Dodecyl Sulfate; D014642:Vanilmandelic Acid; D003404:Creatinine; D006719:Homovanillic Acid