Na+(Li+)/H+ antiporter in Pseudomonas aeruginosa and effect of Li+ on cell growth.
Everted membrane vesicles were prepared from cells of Pseudomonas aeruginosa, and cation/H+ antiport was measured. We observed activities of Na+/H+ antiport, Li+/H+ antiport and K+/H+ antiport. Judging from the competition pattern, it seems that there are at least two types of antiporter, a Na+(Li+)/H+ antiporter and a K+/H+ antiporter. Na+ was a good substrate for the Na+(Li+)/H+ system, whereas Li+ was a poor substrate. Although the K(m) value for Na+ (or Li+) was similar to those in Escherichia coli Na+/H+ antiporters, the Vmax value for Na+ (or Li+) was much smaller in the P. aeruginosa antiporter than in the E. coli antiporters. Growth of P. aeruginosa was strongly inhibited by 0.4 M LiCl, but not by NaCl or KCl.
Inaba
K
K
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Utsugi
J
J
Kuroda
T
T
Tsuda
M
M
Tsuchiya
T
T
D000584:Amiloride / Q000494:pharmacology; D017920:Antiporters / Q000378:metabolism; D001693:Biological Transport, Active; D007700:Kinetics; D008094:Lithium / Q000378:metabolism / Q000494:pharmacology*; D027961:Potassium-Hydrogen Antiporters; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000037:antagonists & inhibitors / Q000378:metabolism*
D017920:Antiporters; D027961:Potassium-Hydrogen Antiporters; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride; D008094:Lithium; D012964:Sodium