Fre-2, a locus closely linked to Fv-2, is rearranged in some erythroleukemias induced by Friend murine leukemia virus.
Friend murine leukemia virus (F-MuLV) induces leukemia by integration into the cellular genome, thereby changing the structure of expression of cellular oncogenes. Here we describe a new F-MuLV integration site Fre-2 isolated from splenic DNA of an erythroleukemic animal. This site has been found rearranged in 5 out of 63 additional tumors; however, no F-MuLV proviruses could be detected in the vicinity of the rearrangement sites in these 5 cases. The rearrangements represented closely clustered chromosomal breakpoints, presumably chromosomal translocations. Exons transcribed into differentially spliced mRNAs of 1.9 and 3.7 kb have been found near the breakpoint. No sequences that are homologous to Fre-2 could be found in databases.
Eisel
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D
Institute of Medical Virology, Justus Liebig University, Giessen, Germany.
Veit
M
M
Friedrich
U
U
Pass
M
M
Sels
F T
FT
Friedrich
R W
RW
D000818:Animals; D010582:Bacteriophage lambda; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005622:Friend murine leukemia virus; D015870:Gene Expression; D015321:Gene Rearrangement; D008040:Genetic Linkage; D015698:Genomic Library; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000821:virology*; D008297:Male; D051379:Mice; D009928:Organ Specificity; D015183:Restriction Mapping; D013154:Spleen / Q000473:pathology; D013160:Splenic Neoplasms / Q000235:genetics* / Q000821:virology*; D016662:Virus Integration
D004273:DNA, Neoplasm; D004279:DNA, Viral