Peptide-based bovine leukemia virus (BLV) vaccine that induces BLV-Env specific Th-1 type immunity.
In controlling retrovirus infection and replication, cell-mediated immunity (CMI) is considered to be effective. To develop a synthetic peptide vaccine capable of inducing CMI, mannan-coated liposome encapsulating 20-mer synthetic peptide, spanning the 98-117 amino acids of bovine leukemia virus (BLV) envelope glycoprotein (Env) gp51 was constructed and inoculated to BALB/c mice. The liposome induced specific delayed-type hypersensitivity, lymphocyte proliferative responses, and a weak cytotoxic lymphocyte response. The spleen cells from the immunized mice produced a large amount of IFN-gamma and IL-2, whereas they released neither IL-4 or IL-10. Mannan-coated liposome containing two kinds of peptides (the 121-140 and 142-161 regions of BLV Env gp51) also induced peptide-specific lymphocyte proliferative response and IFN-g production in C57BL/6 mice. Thus, the synthetic T-cell epitope peptide-liposome system augmented a strong Th-1 type immunity in mice.
Ohishi
K
K
Laboratory of Gene Technology and Safety, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Kabeya
H
H
Amanuma
H
H
Onuma
M
M
D000595:Amino Acid Sequence; D000818:Animals; D019496:Cancer Vaccines; D002417:Cattle; D004337:Drug Carriers; D016583:Enzootic Bovine Leukosis / Q000276:immunology* / Q000517:prevention & control*; D015686:Gene Products, env / Q000276:immunology*; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
D019496:Cancer Vaccines; D004337:Drug Carriers; D015686:Gene Products, env; D008081:Liposomes; D010446:Peptide Fragments; D014614:Vaccines, Synthetic; D014765:Viral Vaccines