Conditionally immortalized cell lines with differentiated functions established from temperature-sensitive T-antigen transgenic mice.
A variety of cell lines with differentiated functions are required to study tissue functions at cellular and molecular levels. Using transgenic mice harbouring ubiquitously expressing the temperature-sensitive T-antigen (ts T-antigen) gene of simian virus 40 (SV40), many cell lines were generated. The properties of these established lines suggested that their growth was dependent on T-antigen, and that they retained some of the differentiated functions of each particular tissue. A possible use of these cell lines for tissue functions in vitro is discussed.
Obinata
M
M
Department of Cell Biology, Institute of Development, Tohoku University, Sendai, Japan. mobinata@idac.tohoku.ac.jp
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D002461:Cell Line, Transformed / Q000502:physiology; D002472:Cell Transformation, Viral / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D013696:Temperature
D000957:Antigens, Viral, Tumor