Cytoprotective effect of ulinastatin on LLC-PK1 cells treated with antimycin A, gentamicin, and cisplatin.
The cytoprotective effect of ulinastatin was studied in LLC-PK1 cells treated with antimycin A, gentamicin, or cisplatin. All of the three agents induced a concentration-dependent increase in the release of lactate dehydrogenase and a decrease in the amount of remaining protein. In the cell injury models treated with 1.5 microM antimycin A, 10 mM gentamicin, and 0.3 mM cisplatin, ulinastatin tended to show a cytoprotective effect at a concentration of 3,000 U/ml and provided a significant protective effect at 10,000 U/ml. LLC-PK1 cells treated with 0.3 mM cisplatin, bovine serum albumin, and alpha1-acid glycoprotein at a concentration of 3.54 mg/ml, which is a comparable protein concentration to that of 10,000 U/ml ulinastatin, showed no protective effect but rather enhanced cell injury. These results suggest that ulinastatin exerts a direct protective effect on LLC-PK1 cells against various renal toxicities.
Ishibashi
M
M
Fuji Central Research Laboratory, Mochida Pharmaceutical Co., Ltd., Gotemba, Shizuoka, Japan.
Yamasaki
F
F
Nakakuki
M
M
Shinkawa
T
T
Mizota
M
M
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors / Q000633:toxicity; D000968:Antimycin A / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000970:Antineoplastic Agents / Q000037:antagonists & inhibitors / Q000633:toxicity; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000633:toxicity; D005839:Gentamicins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D006023:Glycoproteins / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018374:LLC-PK1 Cells; D009961:Orosomucoid / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013552:Swine; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D005839:Gentamicins; D006023:Glycoproteins; D009961:Orosomucoid; D011480:Protease Inhibitors; D012710:Serum Albumin, Bovine; D000968:Antimycin A; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D000118:Acetylglucosaminidase; C028665:urinastatin; D002945:Cisplatin