[Fast thin-layer densitometric determination of the nootropic piracetam in biological material].
After oral doses of piracetam this drug is detectable in serum as well as in urine after formation of the red Fe(III)-hydroxamatecomplex by thin-layer densitometry. Detection in serum requires extraction with a mixture of dichloromethane/ methanol, while urine can be used directly after dilution. The limit of quantification in urine is 100 micrograms/ml and in serum 4.0 micrograms/ml. The new method was validated by HPLC. The coefficient of correlation was 0.9999% for determination in urine and 0.9986% for determination in serum.
Bockhard
H
H
Institut für Pharmazie, Friedrich-Schiller-Universität, Jena, Germany.
Oelschläger
H
H
Pooth
R
R
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003720:Densitometry; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018697:Nootropic Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D010889:Piracetam / Q000032:analysis* / Q000097:blood / Q000652:urine
D018697:Nootropic Agents; D010889:Piracetam