title: Permeability and metabolic properties of a trophoblast cell line (HRP-1) derived from normal rat placenta.
abstract: The HRP-1 cell line is derived from normal rat placenta and appears morphologically similar to and retains characteristic expression of cellular markers of labyrinthine trophoblast cells. In this study, monolayers of HRP-1 cells grown on permeable supports were evaluated as a potential in vitro system to study trophoblast transport and metabolism. The cell line was shown to express and retain functional activity of the predominant placental cytochrome P450 isozyme, CYP1A1. Additionally, the HRP-1 cells retain functional activity of angiotensin I converting enzyme and carboxypeptidase N-like enzyme, peptidases characteristic of the trophoblast. The permeation of several hydrophilic, inert markers across the HRP-1 monolayers was observed to be dependent on effective molecular size and to be passive in nature. Functional asymmetry of the HRP-1 cells was illustrated by the predominant permeation of linoleic acid in the apical-to-basolateral direction across the monolayers. Transferrin passage across HRP-1 monolayers was concentration-dependent, was bidirectional, and could be inhibited by unlabeled transferrin, features typical of the trophoblast transport system for transferrin. Collectively, these properties suggest that the HRP-1 cell line may provide a useful tool for evaluating some of the permeability and metabolic properties of the trophoblast.
authors.affiliation: Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence 66047, USA.
authors.forename: F
authors.initials: F
authors.lastname: Shi
authors.forename: M J
authors.initials: MJ
authors.lastname: Soares
authors.forename: M
authors.initials: M
authors.lastname: Avery
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: K L
authors.initials: KL
authors.lastname: Audus
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D015415:Biomarkers; D002250:Carbon Radioisotopes; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000502:physiology*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003879:Dermatologic Agents / Q000493:pharmacokinetics; D003911:Dextrans / Q000493:pharmacokinetics; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins / Q000493:pharmacokinetics; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000493:pharmacokinetics; D008353:Mannitol / Q000493:pharmacokinetics; D008727:Methotrexate / Q000493:pharmacokinetics; D008861:Microsomes / Q000201:enzymology; D010078:Oxazines / Q000378:metabolism; D051381:Rats; D013395:Sucrose / Q000493:pharmacokinetics; D014168:Transferrin / Q000493:pharmacokinetics; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism*; D014508:Urea / Q000493:pharmacokinetics
chemical_list: D015415:Biomarkers; D002250:Carbon Radioisotopes; D003879:Dermatologic Agents; D003911:Dextrans; D005452:Fluoresceins; D008041:Linoleic Acids; D010078:Oxazines; D014168:Transferrin; D008353:Mannitol; D013395:Sucrose; C007649:ethoxyresorufin; D014508:Urea; D019787:Linoleic Acid; D019363:Cytochrome P-450 CYP1A1; D019793:Fluorescein; D008727:Methotrexate