[Detection of c-Ki-ras oncogene codon 12 point mutations in lung carcinoma, gastric carcinoma and hepatic carcinoma].
DNA extracted from paraffin-embedded tissues of 30 lung carcinomas, 40 gastric carcinomas and 22 hepatocellular carcinomas was tested for the presence of c-Ki-ras codon 12 point mutation by PCR-RFLP technique. The results showed that 20% (6/30) lung carcinomas and 2.5% (1/40) gastric carcinomas had Ki-ras codon 12 point mutation, but non of the 22 hepatocellular carcinomas displayed this point mutation. Among 6 cases of lung carcinomas of mutation, 4 were squamous carcinomas and 2 were adenocarcinomas.
Cui
Q
Q
PUMC Hospital, CAMS and PUMC, Beijing.
Wang
Z
Z
Chen
J
J
Liu
T
T
D000230:Adenocarcinoma / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D003062:Codon / Q000235:genetics; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013274:Stomach Neoplasms / Q000235:genetics*
D003062:Codon