Dictyostelium development in the absence of cAMP.
Adenosine 3',5'-monophosphate (cAMP) and cAMP-dependent protein kinase (PKA) are regulators of development in many organisms. Dictyostelium uses cAMP as an extracellular chemoattractant and as an intracellular signal for differentiation. Cells that are mutant in adenylyl cyclase do not develop. Moderate expression of the catalytic subunit of PKA in adenylyl cyclase-null cells led to near-normal development without detectable accumulation of cAMP. These results suggest that all intracellular cAMP signaling is effected through PKA and that signals other than extracellular cAMP coordinate morphogenesis in Dictyostelium.
Wang
B
B
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030, USA.
Kuspa
A
A
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004023:Dictyostelium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D009024:Morphogenesis; D015398:Signal Transduction; D014170:Transformation, Genetic
D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D000262:Adenylyl Cyclases