Single molecular functional assay of ferritin arrays.
In situ functional assay of each ferritin molecule in single-layer 2D arrays for horse spleen apoferritin and recombinant horse L- and human H-apoferritins was conducted by observing the iron-cores formed in the arrays by TEM. The study of the time-course, pH-dependence, and temperature-dependence of the function confirmed the iron-core formation to be due to the native function of apoferritins in array. Dark-field TEM imaging revealed that there was crystallinity in the cores in the array of recombinant human H-apoferritin. This iron-core formation was perfectly preserved in the array even after 3 months of storage at room temperature and low humidity. Moreover, about 50% of the function was found to remain in the array after it was exposed to 150 degrees C in vacuum for 1 hr.
Yamaki
M
M
Nagayama Protein Array Project, ERATO, JRDC, Tsukuba, Japan.
D000818:Animals; D001052:Apoferritins / Q000737:chemistry / Q000648:ultrastructure*; D005293:Ferritins / Q000737:chemistry / Q000648:ultrastructure*; D006736:Horses; D006801:Humans; D007501:Iron / Q000032:analysis; D007700:Kinetics; D008854:Microscopy, Electron; D011994:Recombinant Proteins / Q000737:chemistry / Q000648:ultrastructure; D012996:Solutions; D013154:Spleen / Q000378:metabolism
D011994:Recombinant Proteins; D012996:Solutions; D005293:Ferritins; D001052:Apoferritins; D007501:Iron