Glucose-dependent transcriptional regulation of the human sucrase-isomaltase (SI) gene.
We have previously shown that the transcription of the human sucrase-isomaltase (SI) gene was negatively regulated by glucose. Using two clonal metabolic variants of the human colon adenocarcinoma cell line Caco-2 we demonstrate here that: 1) although similar growth-related variations of phosphoenolpyruvate carboxykinase (PEPCK), frutose 1,6-diphosphatase (F1, 6-dPase), pyruvate kinase (PK) and SI mRNA levels are observed, only F1,6-dPase, PK and SI mRNA levels vary in the same way in response to modifications of glucose utilization; and 2) regulatory elements responsible for the glucose-dependent transcription of the SI gene are located within the -370/+30 region of the promoter.
Rodolosse
A
A
Unité de Recherches sur la Différenciation Cellulaire Intestinale, INSERM U178, Villejuif, France.
Carrière
V
V
Chantret
I
I
Lacasa
M
M
Zweibaum
A
A
Rousset
M
M
D002454:Cell Differentiation; D015971:Gene Expression Regulation, Enzymologic; D005947:Glucose / Q000378:metabolism; D006801:Humans; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D013394:Sucrase-Isomaltase Complex / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D013394:Sucrase-Isomaltase Complex; D005947:Glucose