title: Cloning and chromosomal localization of a gene (GPR18) encoding a novel seven transmembrane receptor highly expressed in spleen and testis.
abstract: Using the techniques of relaxed stringency polymerase chain reaction and genomic library screening, we have isolated homologous canine and human genes that encode a novel putative seven transmembrane G-protein-linked receptor. The gene encodes an open reading frame (ORF) of 993 bp. The sequences of the canine and human ORFs are highly conserved, sharing 89% nucleotide identity and 92% amino acid similarity between the two species. Northern blot analysis demonstrates that mRNA transcripts of the gene are abundantly expressed in testis and spleen with a lesser degree of expression observed in several other tissues associated with endocrine and immunologic/hematologic function. The gene, designated GPR18, was localized to human chromosome 13q32 using fluorescence in situ hybridization.
authors.affiliation: Department of Surgery, University of Michigan, Ann Arbor 48109, USA.
authors.forename: I
authors.initials: I
authors.lastname: Gantz
authors.forename: A
authors.initials: A
authors.lastname: Muraoka
authors.forename: Y K
authors.initials: YK
authors.lastname: Yang
authors.forename: L C
authors.initials: LC
authors.lastname: Samuelson
authors.forename: E M
authors.initials: EM
authors.lastname: Zimmerman
authors.forename: H
authors.initials: H
authors.lastname: Cook
authors.forename: T
authors.initials: T
authors.lastname: Yamada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004285:Dogs; D019204:GTP-Binding Proteins / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011544:Pseudogenes; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; C107515:GPR18 protein, human; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D019204:GTP-Binding Proteins