Mutational analysis of the structure-function relationship of the leukemogenic membrane glycoprotein (GP55) of Friend spleen focus-forming virus (F-SFFV).
Friend spleen focus-forming virus (F-SFFV) causes acute erythroleukemia in adult mice. F-SFFV encodes an envelope protein-like membrane glycoprotein called gp55 in its defective env gene. Gp55 is responsible for the early stage of leukemogenesis by F-SFFV by specifically binding to and activating the murine erythropoietin receptor (EPO-R). Gp55 has a polytropic env sequence in its N-terminal portion. This portion probably contains the binding site for the EPO-R. In order to obtain a clue for the structure of the binding site to the EPO-R, we isolated and analyzed many spontaneous revertant F-SFFVs which derived from the non-leukemogenic mutant F-SFFV having an ecotropic env sequence instead of the polytropic env sequence in its gp55 gene.
Amanuma
H
H
Laboratory of Gene Technology and Safety, Tsukuba Life Science Center, Ibaraki, Japan.
Yugawa
T
T
Watanabe
N
N
Ikawa
Y
Y
D000818:Animals; D000831:Animals, Newborn; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000378:metabolism; D015686:Gene Products, env / Q000096:biosynthesis / Q000378:metabolism; D015752:Genes, env; D004915:Leukemia, Erythroblastic, Acute / Q000821:virology; D051379:Mice; D008811:Mice, Inbred DBA; D008970:Molecular Weight; D011086:Polycythemia; D017467:Receptors, Erythropoietin / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D013155:Spleen Focus-Forming Viruses / Q000235:genetics / Q000502:physiology*; D013163:Splenomegaly; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000378:metabolism
D004279:DNA, Viral; D015686:Gene Products, env; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C078780:glycoprotein gp55, Friend spleen focus-forming virus