Induction of ABA 8'-hydroxylase by (+)-S-, (-)-R- and 8'-8'-8'-trifluoro-S-abscisic acid in suspension cultures of potato and Arabidopsis.
Suspension cultures of potato and Arabidopsis were incubated with 50 microM of (+)-ABA and (-)-ABA for 3 hr. These pretreatments were found to increase the rate, by two- to seven-fold, of formation of [2H6] phaseic acid (PA) from [2H6] ABA, applied in a subsequent incubation. Pretreatment with trifluoro-ABA had a higher efficacy, increasing the rate of conversion 15-fold. Suspension cell cultures that had been dehydrated and then rehydrated in the presence of [2H6] ABA displayed a much lower enhancement of PA formation. We conclude that ABA induces its own oxidative catabolism in suspension cultures.
Windsor
M L
ML
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824, USA.
Zeevaart
J A
JA
D000040:Abscisic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology*; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D003890:Desiccation; D003903:Deuterium; D004790:Enzyme Induction / Q000187:drug effects; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D010940:Plant Proteins; D011198:Solanum tuberosum / Q000201:enzymology*; D013237:Stereoisomerism
D010940:Plant Proteins; C107205:8',8',8'-trifluoroabscisic acid; D000040:Abscisic Acid; D003577:Cytochrome P-450 Enzyme System; D003903:Deuterium; D006899:Mixed Function Oxygenases; C107203:abscisic acid 8'-hydroxylase