title: The bactericidal effects of dental ultrasound on Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. An in vitro investigation.
abstract: This study investigated the possible bactericidal acoustic effects of the dental ultrasonic scaler. Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis suspensions, were subjected to the vibrations of a Cavitron P1 insert for 2.5 and 5.0 min in an acoustically-simulated pocket model and the survivors enumerated. The extent of any cavitation occurring within the pocket model to which the statistically significant bactericidal activity observed might be attributed, was determined by 'sonoluminescence', which was then investigated by photomultiplication techniques. However, these failed to detect any sonoluminescence within the pocket space and, moreover, the necessary deflection of the water coolant away from the insert tip, to avoid flooding of the experimental pocket, proved to result in temperatures of 47.6 degrees C and 52.3 degrees C at the respective time intervals, and thereby constituted an alternative possible bactericidal mechanism. Examination of the effects of such temperature changes on the target bacteria then revealed statistically significant differences in the viable counts of both microorganisms after 5.0-min periods, and as such were comparable to those previously detected in relation to the pocket model. Whilst it must be presumed that the bacteriolytic effect observed in the main investigation was due to the incidental temperature changes, in the absence of acoustic cavitation the influence of any associated acoustic microstreaming cannot be discounted. Further investigations to assess the bactericidal potential of acoustic phenomena using a modified experimental to exclude any hyperthermic effects are therefore necessary.
authors.affiliation: Department of Periodontology, Eastman Dental Institute, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: O'Leary
authors.forename: A M
authors.initials: AM
authors.lastname: Sved
authors.forename: E H
authors.initials: EH
authors.lastname: Davies
authors.forename: T G
authors.initials: TG
authors.lastname: Leighton
authors.forename: M
authors.initials: M
authors.lastname: Wilson
authors.forename: J B
authors.initials: JB
authors.lastname: Kieser
mesh_terms: D000162:Acoustics; D016976:Aggregatibacter actinomycetemcomitans / Q000254:growth & development*; D015169:Colony Count, Microbial; D012534:Dental Scaling / Q000295:instrumentation*; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced; D008163:Luminescent Measurements; D008953:Models, Anatomic; D010514:Periodontal Pocket / Q000382:microbiology / Q000628:therapy; D016966:Porphyromonas gingivalis / Q000254:growth & development*; D013997:Time Factors; D014464:Ultrasonic Therapy / Q000295:instrumentation*; D014732:Vibration; D014867:Water
chemical_list: D014867:Water