title: Intron-length polymorphism at the actin gene locus mac-1: a genetic marker for population studies in the marine mussels Mytilus galloprovincialis Lmk. and M. edulis L.
abstract: A novel intron-length polymorphism at the actin gene locus mac-1 is here reported and used as a genetic marker for population studies in mussels of the genus Mytilus. Two closely related genes subsequently identified as alleles, mac-1a1 and mac-1b1, from a genomic library of M. galloprovincialis were partially cloned and sequenced. They mainly differed from each other by a 65-bp insertion within their first intron. Polymerase chain reaction (PCR) primers were designed outside the insertion. The PCR analysis of 166 individual mussels from M. galloprovincialis and M. edulis populations revealed three size-classes of alleles or allelomorphs, two of which were of the expected sizes for mac1a1 and mac-1b1. One allelomorph was absent from M. edulis samples, although it was present at substantial frequencies in M. galloprovincialis populations. The frequencies of the two other allelomorphs significantly differed between M. galloprovincialis and M. edulis populations. The comparison of six mac-1 intron sequences over 277 bp showed at once that allelomorphs encompassed alleles differing from one another by substantial numbers of mutations, and that identical alleles were present in both M. galloprovincialis and M. edulis individuals, a probable result of the recent introgression between the two species.
authors.affiliation: Laboratoire Gńome et populations, UPR CNRS 9060, Génétique des Populations marines, Station Méditerranénne de l'Environnement Littoral, Quai de la Daurade, Sete, France.
authors.forename: M
authors.initials: M
authors.lastname: Ohresser
authors.forename: P
authors.initials: P
authors.lastname: Borsa
authors.forename: C
authors.initials: C
authors.lastname: Delsert
mesh_terms: D000199:Actins / Q000235:genetics*; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics*; D004739:England; D005602:France; D005819:Genetic Markers; D005843:Geography; D006239:Haplotypes; D007438:Introns / Q000235:genetics*; D008386:Marine Biology; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D000199:Actins; D005819:Genetic Markers