[A study of the expression and localization of tumor necrosis factor mRNA in small intestine of rats after severe burn].
In this study, the dot blotting and in situ hybridization, collaborated with determination of contents of endotoxin and TNF in plasma, were used to study the expression and localization of TNF mRNA in the intestine. The results showed that, the expression of TNF mRNA in the small intestine increased rapidly and reached maximum at 6 h postburn, being 5.56 times higher than that of normal control. The TNF mRNA-producing cells in normal animal were mononuclear phagocytes located in lamina propria. After burn, the number of positive cells in lamina propria increased significantly, and the TNF gene-expressing cells were vascular endothelium. Many positive cells were found in the crypts of the intestine. It suggested that the expression of TNF correlated well with bacterial translocation in the early stage of burn. The mononuclear phagocytes and endothelial cells located in lamina propria and interstitial tissue were the chief TNF mRNA-producing cells in the intestine. The overproduction of TNF could provoke microvascular injury or induce the production of oxygen free radical to damage the gut mucosal barrier.
Yuan
J
J
Institute of Burn Research, South-western Hospital, Third Military Medical University, Chongqing.
Xiao
G
G
Zhou
L
L
D000818:Animals; D018988:Bacterial Translocation; D002056:Burns / Q000378:metabolism*; D005260:Female; D007421:Intestine, Small / Q000378:metabolism* / Q000382:microbiology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha