Diffusion of microinjected markers across the parasitophorous vacuole membrane in cells infected with Eimeria nieschulzi (Coccidia, Apicomplexa).
Cells infected with the intracellular parasite Eimeria nieschulzi were microinjected with lucifer yellow (457 Da), biocytin lucifer yellow (850 Da) and dextranrhodamine (10,000 Da). Immediately after injection of a mixture of the markers into the host cell cytoplasm, a differential diffusion pattern was observed in trophozoites and schizonts. Lucifer yellow and biotin lucifer yellow were seen to enter the parasitophorous vacuole, whereas the dextran was excluded. Since these markers cannot permeate cell membranes, this suggests that the Eimerian parasitophorous vacuole acts as a molecular sieve. This method allows the visualization and further characterization of the parasitophorous vacuole in living cells.
Werner-Meier
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R
Technische Universität Dresden, Institut für Zoologie, Abteilung Spezielle Zoologie, Dresden, Germany.
Entzeroth
R
R
D000818:Animals; D002460:Cell Line; D003911:Dextrans / Q000378:metabolism*; D004058:Diffusion; D004539:Eimeria / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D007202:Indicators and Reagents / Q000378:metabolism*; D007546:Isoquinolines / Q000378:metabolism*; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D008845:Microinjections; D051381:Rats; D012235:Rhodamines / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism
D003911:Dextrans; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007546:Isoquinolines; D012235:Rhodamines; C051311:rhodamine dextran; C017475:lucifer yellow; C013411:biocytin; D008239:Lysine