Identification and partial characterization of beta-1,3-glucanase from virus-infected cucumber cotyledons.
One of the five "pathogenesis-related" (PR) proteins known to accumulate in Cucumis sativus L. cv. Laura and to react hypersensitively to tobacco necrosis virus (TNV) was shown to to have beta-1,3-glucanase activity. The TNV-induced acidic beta-1,3-glucanase activity increased 6-fold after infection and had extracellular localization and estimated M(r) of 25,700. The beta-1,3-glucanase activity was investigated with a new method of activity staining using a conjugated substrate in overlay gels. By using the antiserum against tobacco beta-1,3-glucanase purified to homogeneity, a close serological relationship was demonstrated between cucumber and tobacco beta-1,3-glucanases on immunoblots.
Repka
V
V
Laboratory of Molecular Biology and Virology, Complex Research Institute of Viticulture and Enology, Bratislava, Slovak Republic.
Tamás
L
L
Fischerová
I
I
Fric
F
F
D043326:Glucan 1,3-beta-Glucosidase; D007120:Immunochemistry; D008970:Molecular Weight; D010940:Plant Proteins / Q000302:isolation & purification; D010942:Plant Viruses / Q000472:pathogenicity*; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology / Q000821:virology; D014675:Vegetables / Q000201:enzymology* / Q000821:virology*; D014774:Virulence; D001617:beta-Glucosidase / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*
D010940:Plant Proteins; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase