Serological relationships between tobacco necrosis virus-inducible cucumber peroxidase and corresponding peroxidase isoenzymes in other Cucurbitaceae.
Pathogenesis-related peroxidases (PRXs), found in leaves of different cucurbits infected with tobacco necrosis virus (TNV), were compared serologically using a highly specific rabbit antiserum raised against PRX purified from TNV-infected cotyledons of Cucumis sativus L., cv. Laura. After native polyacrylamide gel electrophoresis (PAGE) of intercellular washing fluid extracts from a large number of species of this family, immunoblot analysis revealed the presence of cross-reacting protein bands in all species tested. Despite the serological relatedness, the analysis also revealed that the antiserum recognized only fast-moving PRX isoenzymes localized in the apoplast space.
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Laboratory of Molecular Biology and Virology, Complex Research Institute of Viticulture and Enology, Bratislava, Slovak Republic.
D000818:Animals; D000906:Antibodies; D003429:Cross Reactions; D004790:Enzyme Induction; D015151:Immunoblotting; D007527:Isoenzymes / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D010544:Peroxidases / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D010942:Plant Viruses / Q000472:pathogenicity*; D011817:Rabbits; D014675:Vegetables / Q000201:enzymology* / Q000821:virology*
D000906:Antibodies; D007527:Isoenzymes; D010544:Peroxidases