title: Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates.
abstract: Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.
authors.affiliation: Department of Chemistry, King's College London, Strand, London WC2R 2LS, UK.
authors.forename: C B
authors.initials: CB
authors.lastname: Reese
authors.forename: Q
authors.initials: Q
authors.lastname: Song
mesh_terms: D000641:Ammonia / Q000737:chemistry*; D008623:Mercaptoethanol / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry*; D013873:Thionucleotides / Q000737:chemistry*
chemical_list: D013873:Thionucleotides; D008623:Mercaptoethanol; D013455:Sulfur; D000641:Ammonia