title: Differential regulation of phosphoinositide 3-kinase adapter subunit variants by insulin in human skeletal muscle.
abstract: The role of phosphoinositide 3-kinase (PI 3-kinase) in insulin signaling was evaluated in human skeletal muscle. Insulin stimulated both antiphosphotyrosine-precipitable PI 3-kinase activity and 3-O-methylglucose transport in isolated skeletal muscle (both approximately 2-3-fold). Insulin stimulation of 3-O-methylglucose transport was inhibited by the PI 3-kinase inhibitor LY294002 (IC50 = 2.5 microM). The PI 3-kinase adapter subunits were purified from muscle lysates using phosphopeptide beads based on the Tyr-751 region of the platelet-derived growth factor receptor. Immunoblotting of the material adsorbed onto the phosphopeptide beads revealed the presence of p85alpha, p85beta, p55(PIK)/p55gamma, and p50 adapter subunit isoforms. In addition, p85alpha-NSH2 antibodies recognized four adapter subunit variants of 54, 53, 48, and 46 kDa, the latter corresponding to the p50 splice variant. Serial immunoprecipitations demonstrated that these four proteins were associated with a large proportion of the total PI 3-kinase activity immunoprecipitated by p85alpha-NSH2 domain antibodies. Antibodies to p85beta, p55(PIK)/p55gamma, and the p50 adapter subunit also immunoprecipitated PI 3-kinase activity from human muscle lysates. A large proportion of the total cellular pool of the 53-kDa variant, p50, and p55(PIK) was present in antiphosphotyrosine immunoprecipitates from unstimulated muscle, whereas these immunoprecipitates contained only a very small proportion of the cellular pool of p85alpha, p85beta, and the 48-kDa variant. Insulin greatly increased the levels of the 48-kDa variant in antiphosphotyrosine immunoprecipitates and caused smaller -fold increases in the levels of p85alpha, p85beta, and the 53-kDa variant. The levels of p50 and p55(PIK) were not significantly changed. These properties indicate mechanisms by which specificity is achieved in the PI 3-kinase signaling system.
authors.affiliation: Department of Biochemistry, University College London, Gower Street, London WC1E 6BT, United Kingdom. p.shepherd@biochem.ucl.ac.uk
authors.forename: P R
authors.initials: PR
authors.lastname: Shepherd
authors.forename: B T
authors.initials: BT
authors.lastname: Navé
authors.forename: J
authors.initials: J
authors.lastname: Rincon
authors.forename: L A
authors.initials: LA
authors.lastname: Nolte
authors.forename: A P
authors.initials: AP
authors.lastname: Bevan
authors.forename: K
authors.initials: K
authors.lastname: Siddle
authors.forename: J R
authors.initials: JR
authors.lastname: Zierath
authors.forename: H
authors.initials: H
authors.lastname: Wallberg-Henriksson
mesh_terms: D019325:3-O-Methylglucose / Q000378:metabolism; D000328:Adult; D002867:Chromones / Q000494:pharmacology; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D009025:Morpholines / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases; D010748:Phosphopeptides / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011487:Protein Conformation; D015398:Signal Transduction; D018909:src Homology Domains
chemical_list: D002867:Chromones; D004791:Enzyme Inhibitors; D007328:Insulin; D009025:Morpholines; D010748:Phosphopeptides; D019325:3-O-Methylglucose; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor)