Comparative study of antiphospholipid antibody detection in eleven Belgian laboratories.
Twenty-six plasma samples have been sent to 11 different Belgian laboratories in order to detect the presence of antiphospholipid antibodies, either by immunological methods and/or by coagulation tests. A good concordance between laboratories was observed for coagulation tests. Laboratories using detection tests and performing mixing procedures and neutralisation procedures displayed the highest sensitivity as compared with laboratories which did not perform one of these two latter procedures. The concordance between laboratories for the immunological methods was much worse as compared with coagulation tests. This may be attributable either to an intrinsic problem of the immunological tests or to a selection bias due the fact that the plasmas used in this study were selected in coagulation laboratories only where the chance to find a lupus anticoagulant positive/ELISA antiphospholipid negative sample is high.
Capel
P
P
Hôpital Erasme, Université Libre de Bruxelles.
Arnout
J
J
Cauchie
P
P
Chatelain
B
B
Criel
A
A
David
J L
JL
Fondu
P
P
Jochmans
K
K
Lavenne
E
E
Lust
A
A
Van der Planken
M
M
D000328:Adult; D000368:Aged; D017152:Antibodies, Antiphospholipid / Q000097:blood*; D001530:Belgium; D001780:Blood Coagulation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D007753:Laboratories / Q000592:standards; D016682:Lupus Coagulation Inhibitor / Q000097:blood; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D010314:Partial Thromboplastin Time; D010743:Phospholipids; D011237:Predictive Value of Tests; D015983:Selection Bias; D012680:Sensitivity and Specificity
D017152:Antibodies, Antiphospholipid; D016682:Lupus Coagulation Inhibitor; D010743:Phospholipids