Developmental expression pattern of Stra6, a retinoic acid-responsive gene encoding a new type of membrane protein.
Retinoic acid plays important roles in development, growth and differentiation by regulating the expression of target genes. A new retinoic acid-inducible gene, Stra6, has been identified in P19 embryonal carcinoma cells using a subtractive hybridization cDNA cloning technique. Stra6 codes for a very hydrophobic membrane protein of a new type, which does not display similarities with previously characterized integral membrane proteins. Stra6, which exhibits a specific pattern of expression during development and in the adult, is strongly expressed at the level of blood-organ barriers. Interestingly, in testis Sertoli cells, Stra6 has a spermatogenic cycle-dependent expression which is lost in testes of RAR alpha null mutants where Stra6 is expressed in all tubules. We suggest that the Stra6 protein may be a component of an as yet unidentified transport machinery.
Bouillet
P
P
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Collège de France. C.U. de Strasbourg, France.
Sapin
V
V
Chazaud
C
C
Messaddeq
N
N
Décimo
D
D
Dollé
P
P
Chambon
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018236:Carcinoma, Embryonal / Q000235:genetics; D002831:Choroid Plexus / Q000378:metabolism; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000172:deficiency; D000072482:Retinoic Acid Receptor alpha; D047488:Retinoid X Receptors; D012708:Sertoli Cells / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D014157:Transcription Factors / Q000172:deficiency; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D015017:Yolk Sac / Q000378:metabolism
D008565:Membrane Proteins; C000607072:Rara protein, mouse; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; D047488:Retinoid X Receptors; C108001:Stra6 protein, mouse; D014157:Transcription Factors; D014212:Tretinoin