Alteration of cartilage metabolism by cells from osteoarthritic bone.
OBJECTIVE
To determine whether bone cells alter cartilage metabolism.


METHODS
Bone cell cultures were established using explants obtained from the hip and knee joints of 9 patients with osteoarthritis (OA) and 6 subjects without arthritis (nonarthritic [NA]). NA human cartilage biopsy samples were incubated in the presence or absence of bone-derived cells, and the effects on glycosaminoglycan (GAG) release from cartilage were measured.


RESULTS
Bone cell cultures secreted osteocalcin (OC) and did not contain cells expressing leukocyte common antigen. None of the 8 cultures established from NA bone, compared with 17 of 32 from OA bone, significantly altered GAG release from cartilage (P = 0.006). In knees with medial joint damage, 38% of the cultures derived from the medial side of the joint increased GAG release from cartilage. In contrast, 77% of the cultures derived from the lateral side of the joint had an effect on GAG, with 38% increasing and 38% decreasing GAG release. Seven cytokines were measured in OA bone cell supernatants. No significant difference was apparent in the concentration of any one cytokine when supernatants were compared according to their effects on GAG release.


CONCLUSIONS
Bone cells from OA patients can influence cartilage metabolism. This might explain why increased subchondral bone activity can predict cartilage loss.
Westacott
C I
CI
University of Bristol, UK.
Webb
G R
GR
Warnock
M G
MG
Sims
J V
JV
Elson
C J
CJ
D000368:Aged; D002356:Cartilage / Q000378:metabolism*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D008297:Male; D010003:Osteoarthritis / Q000378:metabolism*; D015675:Osteocalcin / Q000378:metabolism
D016207:Cytokines; D006025:Glycosaminoglycans; D015675:Osteocalcin