Roles of N-glycans with alpha2,6 as well as alpha2,3 linked sialic acid in infection by polyoma virus.
Specific glycosylation inhibitors were used to show that N-glycans, and not O-glycans or glycolipids, constitute the major class of receptors on 3T3 cells for polyoma virus. Sialic acid (SA)-specific lectins were used in attempts to confirm the expected SA linkage on N-linked glycans. A lectin specific for alpha2,6 linked SA, a non-receptor type, was slightly more effective in blocking infection than a lectin specific for alpha2,3 linked SA which corresponds to the known receptor specificity. Possible explanations are suggested for this unexpected result.
Chen
M H
MH
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Benjamin
T
T
D017485:1-Deoxynojirimycin / Q000494:pharmacology; D016475:3T3 Cells; D000818:Animals; D037102:Lectins / Q000494:pharmacology; D051379:Mice; D019158:N-Acetylneuraminic Acid / Q000502:physiology*; D011120:Polyomavirus / Q000472:pathogenicity*; D011134:Polysaccharides / Q000502:physiology*; D011956:Receptors, Cell Surface; D011991:Receptors, Virus / Q000502:physiology*; D014415:Tunicamycin / Q000494:pharmacology
D037102:Lectins; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C092488:sialic acid receptor; D014415:Tunicamycin; D017485:1-Deoxynojirimycin; D019158:N-Acetylneuraminic Acid