A universal procedure for primer labelling of amplicons.
Detection and visualisation of nucleic acids is integral to genome analyses. Exponential amplification procedures have provided the means for the manipulation of nucleic acid sequences, which were otherwise inaccessible. We describe the development and application of a universal method for the labelling of any PCR product using a single end-labelled primer. Amplification was performed in a single reaction with the resulting amplicon labelled to a high specific activity. The method was adapted to a wide range of PCRs and significantly reduced the expense of such analyses.
Neilan
B A
BA
School of Microbiology and Immunology, The University of New South Wales, Sydney, NSW 2052, Australia. b.neilan@unsw.edu.au
Wilton
A N
AN
Jacobs
D
D
D017931:DNA Primers; D018900:Dinucleotide Repeats; D005240:Feasibility Studies; D006801:Humans; D008969:Molecular Sequence Data; D010798:Phycocyanin / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D010798:Phycocyanin