title: Sequence and phylogenetic analysis of the cytoplasmic inclusion protein gene of zucchini yellow mosaic potyvirus: its role in classification of the Potyviridae.
abstract: The cytoplasmic inclusion (CI) gene of a Singapore isolate of zucchini yellow mosaic virus (ZYMV-S) was sequenced and compared with CI of 14 other potyviruses. In addition to the consensus sequence GAVGSGKST of nucleotide binding motif (NTBM) which is implicated as a membrane-binding component of the RNA helicase complex, five other conserved motifs were found. Phylogenetic trees were constructed from sequence data for the CI and the coat protein. Similar branching patterns obtained from both CI and coat protein analyses suggests that phylogenetic relationship among potyviruses can be determined using the CI. We propose that phylogenetic analysis of CI gene may be used as an alternative approach for the study of evolution within the family Potyviridae.
authors.affiliation: School of Biological Sciences, National University of Singapore, Kent Ridge, Republic of Singapore.
authors.forename: K C
authors.initials: KC
authors.lastname: Lee
authors.forename: P H
authors.initials: PH
authors.lastname: Mahtani
authors.forename: C G
authors.initials: CG
authors.lastname: Chng
authors.forename: S M
authors.initials: SM
authors.lastname: Wong
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D019143:Evolution, Molecular; D005814:Genes, Viral; D007181:Inclusion Bodies, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D019176:Potyviridae / Q000145:classification* / Q000235:genetics*; D017800:Potyvirus / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D014764:Viral Proteins