title: Ran1 functions to control the Cdc10/Sct1 complex through Puc1.
abstract: We have undertaken a biochemical analysis of the regulation of the G1/S-phase transition and commitment to the cell cycle in the fission yeast Schizosaccharomyces pombe. The execution of Start requires the activity of the Cdc2 protein kinase and the Sct1/Cdc10 transcription complex. Progression through G1 also requires the Ran1 protein kinase whose inactivation leads to activation of the meiotic pathway under conditions normally inhibitory to this process. We have found that in addition to Cdc2, Sct1/Cdc10 complex formation requires Ran1. We demonstrate that the Puc1 cyclin associates with Ran1 and Cdc10 in vivo and that the Ran1 protein kinase functions to control the association between Puc1 and Cdc10. In addition, we present evidence that the phosphorylation state of Cdc10 is altered upon inactivation of Ran1. These results provide biochemical evidence that demonstrate one mechanism by which the Ran1 protein kinase serves to control cell fate through Cdc10 and Puc1.
authors.affiliation: Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724, USA.
authors.forename: M
authors.initials: M
authors.lastname: Caligiuri
authors.forename: T
authors.initials: T
authors.lastname: Connolly
authors.forename: D
authors.initials: D
authors.lastname: Beach
mesh_terms: D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000502:physiology*; D016213:Cyclins / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D005656:Fungal Proteins / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D010449:Peptide Mapping; D010768:Phosphoserine / Q000378:metabolism; D011494:Protein Kinases / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D018797:Cell Cycle Proteins; D016213:Cyclins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C061178:cdc10 protein, S pombe; C087768:puc1 protein, S pombe; D010768:Phosphoserine; D011494:Protein Kinases; C087019:ran1 protein, S pombe; D017346:Protein Serine-Threonine Kinases