Simultaneous determination of amoxycillin and clavulanic acid in pharmaceutical products by HPLC with beta-cyclodextrin stationary phase.
A simple, rapid and accurate method for simultaneous determination of amoxycillin and clavulanic acid using HPLC with beta-cyclodextrin stationary phase was developed. It involves the use of tetraethylammonium acetate (TEAA) as an additive reagent, methanol-buffer solution (pH 4.5) (35:65; v/v) as the mobile phase, detection at 225 mm and chromatogram within 12 min. Linearity and precision of the internal standard method have been obtained. Recoveries ranged from 99.25 to 105.63% for amoxycillin in the synthetic mixture. For clavulanic acid it was from 99.50 to 101.64%. This method is convenient and reproducible for analyses of these two components in different dosage forms.
Tsou
T L
TL
Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan.
Wu
J R
JR
Young
C D
CD
Wang
T M
TM
D000658:Amoxicillin / Q000032:analysis; D019980:Amoxicillin-Potassium Clavulanate Combination; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D002969:Clavulanic Acids / Q000032:analysis; D003505:Cyclodextrins; D004304:Dosage Forms; D004359:Drug Therapy, Combination / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet; D047392:beta-Cyclodextrins
D002021:Buffers; D002969:Clavulanic Acids; D003505:Cyclodextrins; D004304:Dosage Forms; D047392:beta-Cyclodextrins; D019980:Amoxicillin-Potassium Clavulanate Combination; D000658:Amoxicillin; C031215:betadex