Amperometric detection of organic thiols at a tungsten wire electrode following their separation by liquid chromatography.
The amperometric detection of thiols following their separation by reversed-phase chromatography and reaction into a post-column mercury carrier stream is shown to be a sensitive method when using a tungsten wire sensor as the working electrode in a three-electrode flow cell. Five organic thiols (cysteine, homocysteine, reduced glutathione, D,L-penicillamine and 2-mercaptopropionic acid) and thiourea could be separated within approx. 18 min. The analytical performance is comparable, and stability superior, to chemically modified electrodes previously reported.
Hidayat
A
A
Department of Analytical Chemistry, University of New South Wales, Sydney, Australia.
Hibbert
D B
DB
Alexander
P W
PW
D002853:Chromatography, Liquid; D003545:Cysteine / Q000032:analysis / Q000097:blood / Q000652:urine; D004563:Electrochemistry; D004566:Electrodes; D005978:Glutathione / Q000032:analysis / Q000097:blood / Q000652:urine; D006710:Homocysteine / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D010396:Penicillamine / Q000032:analysis / Q000097:blood / Q000652:urine; D012623:Seawater / Q000737:chemistry; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000097:blood / Q000652:urine; D013890:Thiourea / Q000032:analysis; D014414:Tungsten
D013438:Sulfhydryl Compounds; D006710:Homocysteine; D005978:Glutathione; D010396:Penicillamine; D013890:Thiourea; D003545:Cysteine; D014414:Tungsten