title: Qualitative and quantitative measurements of oligonucleotides in gene therapy: Part II in vivo models.
abstract: Part I of this review has already been presented on methods of processing and purification of crude or raw samples in cell-culture or cell free systems. Part II of the review focuses on the in vivo models of determination of input oligonucleotides in both non-primates and primates. Emphasis has been given to the techniques developed for quantification of oligonucleotides or their metabolites from biological samples including blood, plasma, serum, urine and other tissues.
authors.affiliation: Department of Pharmacology, University of Nebraska Medical Center, Omaha 68198-6260, USA.
authors.forename: H K
authors.initials: HK
authors.lastname: Tewary
authors.forename: P L
authors.initials: PL
authors.lastname: Iversen
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D017321:Clinical Trials, Phase I as Topic; D004591:Electrophoresis, Polyacrylamide Gel; D015316:Genetic Therapy; D006801:Humans; D009841:Oligonucleotides / Q000032:analysis* / Q000493:pharmacokinetics / Q000627:therapeutic use; D010761:Phosphorus Radioisotopes; D013050:Spectrometry, Fluorescence; D013462:Sulfur Radioisotopes; D014018:Tissue Distribution
chemical_list: D009841:Oligonucleotides; D010761:Phosphorus Radioisotopes; D013462:Sulfur Radioisotopes