Trophic effects of glicentin on rat small-intestinal mucosa in vivo and in vitro.
To define the role of glicentin the active site of enteroglucagon, we evaluated the trophic effects of recombinant rat glicentin on rat small intestine and IEC-6 cells. In vivo, a significant increase was observed in jejunal wet weight, protein content, DNA content, and alkaline phosphatase activity after the subcutaneous administration of 100 micrograms/kg per day of glicentin for 2 weeks. In the ileum, however, there were no significant differences between the control versus glicentin groups in any of these parameters. Ornithine decarboxylase (ODC) activity 3.5 h after an intraperitoneal injection of glicentin was increased in the jejunal mucosa, but not in the ileal mucosa. In vitro, glicentin, at a dose of more than 100 ng/ml, significantly increased both tritium-thymidine incorporation and the number of IEC-6 cells. These findings indicate that glicentin exerts direct trophic effects on the rat small-intestinal mucosa and on the rat small-intestinal cell line, IEC-6, and that this peptide appears to be an active site of enteroglucagon.
Myojo
S
S
Second Department of Internal Medicine, Shiga University of Medical Science, Japan.
Tsujikawa
T
T
Sasaki
M
M
Fujiyama
Y
Y
Bamba
T
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001835:Body Weight; D002452:Cell Count; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D053771:Glicentin; D005934:Glucagon / Q000378:metabolism / Q000494:pharmacology*; D004763:Glucagon-Like Peptides / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects*; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology*; D011498:Protein Precursors / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010446:Peptide Fragments; D011498:Protein Precursors; D004763:Glucagon-Like Peptides; D053771:Glicentin; D004247:DNA; D005934:Glucagon; D000469:Alkaline Phosphatase