Substance P binding to peripheral blood mononuclear leukocytes in atopic dermatitis.
Substance P has various immunomodulatory effects, including in vitro modification of lymphocyte proliferation and cytokine release. Elevated levels of substance P and increased staining of substance P-positive nerve fibres have been reported in atopic dermatitis patients. We examined fluoresceinated substance P binding to a range of lymphocyte subsets and compared the results in atopic dermatitis, non-atopic psoriasis patients and normal controls. Fluoresceinated substance P and phycoerythrin-labelled monoclonal antibodies to CD3, CD4, CD8, CD57, CD19 and CD14 were incubated in duplicate with Ficoll-Hypaque separated peripheral blood mononuclear leukocytes. With flow cytometry the fluoresceinated substance P-positive cells were identifiable as a peak of positively fluorescent cells, and the percentages of positive cells were measured. We have demonstrated binding of fluoresceinated substance P to all subsets examined, with significantly less binding to atopic dermatitis CD3-, CD8- and CD57-positive cells. This may affect cytokine release and hence be important in the pathogenesis of atopic dermatitis.
Ostlere
L S
LS
Department of Dermatology, St Helier N.H.S. Trust, Surrey, UK.
Gordon
D J
DJ
Ayliffe
M J
MJ
Rustin
M H
MH
Pereira
R S
RS
Holden
C A
CA
D000293:Adolescent; D000328:Adult; D003876:Dermatitis, Atopic / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D019793:Fluorescein; D005452:Fluoresceins; D006801:Humans; D016131:Lymphocyte Subsets; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010799:Phycoerythrin; D011485:Protein Binding; D011565:Psoriasis / Q000097:blood; D013373:Substance P / Q000097:blood*
D005452:Fluoresceins; D010799:Phycoerythrin; D013373:Substance P; D019793:Fluorescein