title: Sorbitol accumulation and transmembrane efflux in osmotically stressed JS1 schwannoma cells.
abstract: Sorbitol accumulation and transmembrane efflux in JS1 schwannoma cells were examined during osmotic stress in the presence of a sorbitol dehydrogenase (SD) inhibitor and following return to iso-osmotic conditions. SD inhibition promoted sorbitol accumulation under hyperglycemic and/or hyperosmotic conditions, and sorbitol efflux during iso-osmotic incubation was prevented by cooling to 4 degrees C or by quinidine. It appears that sorbitol levels in JS1 cells are dependent on SD activity and that sorbitol is rapidly removed upon restoring iso-osmotic conditions.
authors.affiliation: Department of Pathology, School of Medicine, University of California, San Diego, La Jolla 92093-0612, USA. amizisin@ucsd.edu
authors.forename: A P
authors.initials: AP
authors.lastname: Mizisin
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: N A
authors.initials: NA
authors.lastname: Calcutt
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media; D007064:L-Iditol 2-Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D009442:Neurilemmoma / Q000378:metabolism*; D009997:Osmotic Pressure; D011108:Polymers / Q000378:metabolism; D011802:Quinidine / Q000494:pharmacology; D051381:Rats; D013012:Sorbitol / Q000378:metabolism*; D013696:Temperature; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media; D011108:Polymers; C024617:polyol; D013012:Sorbitol; D007064:L-Iditol 2-Dehydrogenase; D011802:Quinidine