Distribution of Ca(2+)-permeable AMPA receptors among cultured rat cerebellar granule cells.
Some AMPA receptors are permeable to Ca2+. It has been suggested that cultured rat cerebellar granule cells express Ca(2+)-permeable AMPA receptors, but their distribution at a single cell level is unknown. We report that AMPA (in the presence of cyclothiazide) induced Ca2+ entry (measured by Mn2+ quench of fura-2 fluorescence) and intracellular Ca2+ increases in cerebellar granule cells in the absence of extracellular Na+, supporting the presence of Ca(2+)-permeable AMPA receptors. Analysis of intracellular Ca2+ signals in single cells demonstrated a heterogeneous distribution of Ca(2+)-permeable AMPA receptors.
Savidge
J R
JR
University of Manchester, School of Biological Sciences, Division of Neuroscience, UK.
Bristow
D R
DR
D000818:Animals; D001581:Benzothiadiazines / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004232:Diuretics; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005456:Fluorescent Dyes; D016257:Fura-2; D008345:Manganese / Q000378:metabolism; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism*; D049993:Sodium Chloride Symporter Inhibitors / Q000494:pharmacology; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
D001581:Benzothiadiazines; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004232:Diuretics; D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D005456:Fluorescent Dyes; D011810:Quinoxalines; D018091:Receptors, AMPA; D049993:Sodium Chloride Symporter Inhibitors; D008345:Manganese; C056723:FG 9041; D016291:Dizocilpine Maleate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; C004639:cyclothiazide; D002118:Calcium; D016257:Fura-2