Structure of in-serum transfecting DNA-cationic lipid complexes.
Noticeable modifications of in-serum transfection efficiency of dioctadecylamidoglycyl-spermine (DOGS)-DNA complexes are observed, depending on DNA condensation conditions. The structures of the complexes are studied, keeping in mind the variability of lipid polymorphism, by cryo-transmission electron microscopy and X-ray diffraction. By increasing both pH and ionic strength, well-organised lamellar structures with a period of 65 A replace supramicellar aggregates. A relationship between the structures and their in-vitro transfection activity is established. Efficiency in the presence of serum is maintained when a lamellar arrangement is involved.
Boukhnikachvili
T
T
Rhône-Poulenc Rorer Gencell, Centre de Recherche de Vitry-Alfortville, Vitry sur Seine, France.
Aguerre-Chariol
O
O
Airiau
M
M
Lesieur
S
S
Ollivon
M
M
Vacus
J
J
D016475:3T3 Cells; D000818:Animals; D002412:Cations; D004247:DNA / Q000097:blood / Q000737:chemistry* / Q000648:ultrastructure; D000431:Ethanol; D005312:Fetal Blood; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry; D008055:Lipids / Q000097:blood / Q000737:chemistry* / Q000235:genetics*; D051379:Mice; D008854:Microscopy, Electron; D012965:Sodium Chloride; D013096:Spermine / Q000031:analogs & derivatives / Q000737:chemistry; D014162:Transfection; D014961:X-Ray Diffraction
D002412:Cations; D008055:Lipids; C061134:dioctadecylamidoglycylspermine; D013096:Spermine; D000431:Ethanol; D012965:Sodium Chloride; D004247:DNA; D005998:Glycine