Inductive capacity of living eye tissues from adult frogs.
The aim of the present work has been to demonstrate the inducing capacity of living homogenous undamaged tissues from adult frogs. Tissues from the eyes of adult frogs Xenopus laevis: retina (R), pigmented epithelium (PE), lens epithelium (LE), and the forebrain (B) as a control, were tested for their inducing capacity using early gastrula ectoderm. To exclude the possibility of ad-mixture of inducing cells in early gastrula ectoderm the transfilter induction technique was used throughout. The results show that the tissues used in the cases of most intense induction tended to induce similar cell types: both R and PE induce R + PE together with adjoining neural cells and secondary lens cells. LE induces lentoids (L) and B cells induce neuroids. In each case epidermis surrounds the explants filled with ectomesenchyme (EM) and melanophores (M). Immunofluorescence reactions clarified the nature of lens cells. This discovery indicates that cells of adult tissues continue throughout life producing substances which are able to promote the appearance of cells of the same type during development. Probably they also serve to mediate interrelations between cells of these tissues, regulating the stability of their differentiation in the adult state, as well.
Lopashov
G V
GV
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Golubeva
O N
ON
Zviadadze
K G
KG
D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation; D004475:Ectoderm / Q000187:drug effects / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology*; D004627:Embryonic Induction / Q000187:drug effects / Q000502:physiology*; D000078404:Epidermal Cells; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000502:physiology; D005123:Eye / Q000737:chemistry; D005775:Gastrula / Q000187:drug effects; D007908:Lens, Crystalline / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D009799:Ocular Physiological Phenomena; D010857:Pigment Epithelium of Eye / Q000737:chemistry / Q000502:physiology; D016548:Prosencephalon / Q000737:chemistry / Q000502:physiology; D012160:Retina / Q000737:chemistry / Q000502:physiology; D014982:Xenopus laevis / Q000502:physiology*