[The effect of the processes of Klebsiella pneumoniae cultivation on the antigenic activity of vaccinal preparations for peroral use].
Whole-cell preparations, obtained from the microbial mass of K. pneumoniae grown by batch and continuous cultivation at a dilution rate of 0.24 and 0.41 hr-1 and used in animal experiments for oral administration, were shown to have different serological activity. The preparation obtained from biomass grown by continuous cultivation at a dilution rate of 0.41 hr-1 proved to be most active regarding the level of hemagglutinating antibodies to K. pneumoniae LPS. At the same time the 360-fold rise of the level of anti-LPS antibodies in rabbit immune sera was observed. On day 258 oral revaccination was made; after that the twofold rise of the level of anti-LPS antibodies in the sera of the animals was observed. These antibody levels exceeded 40-fold those registered before primary immunization, and sufficiently high antibody levels were retained for 4 more months (the term of observation).
Aleksakhina
N N
NN
Basnak'ian
I A
IA
Karabak
V I
VI
Stukalova
N V
NV
Miriasova
L V
LV
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification*; D019149:Bioreactors; D003470:Culture Media; D004306:Dose-Response Relationship, Immunologic; D007114:Immunization / Q000379:methods; D007711:Klebsiella pneumoniae / Q000254:growth & development* / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D011817:Rabbits; D013997:Time Factors
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D003470:Culture Media; D008070:Lipopolysaccharides