The mouse gene for the inducible G-protein-coupled receptor edg-1.
Edg-1, an immediate-early gene induced during the in vitro differentiation of human endothelial cells, encodes a G-protein-coupled receptor (GPR) that signals via the G(i)/mitogen-activated protein kinase (MAP kinase) pathway (Lee, M.-J., Evans, M., and Hla, T. (1996) J. Biol. Chem. 271, 11272-11279). It is a prototypical member of the subfamily of "orphan" receptors that are expressed in the cardiovascular and nervous systems. In this report, the mouse edg-1 gene was cloned and sequenced, and its expression patterns were defined. The edg-1 transcript was expressed in a wide variety of adult tissues including the brain, lung, liver, heart, and spleen. However, during embryogenesis, the edg-1 mRNA was induced late in development (after Embryonic Day 15.5) at centers of ossification. As a first step toward understanding the molecular basis of tissue-specific and inducible expression, the mouse edg-1 gene and its promoter were characterized. The mouse edg-1 gene is composed of two exons and is 4.9 kb in length. The second exon is large and contains the entire coding region and the 3'-untranslated region. The edg-1 promoter is TATA-less and contains GC-rich elements, and transcription initiation occurs from a single start site. The 5'-flanking region of the promoter contains several enhancer elements. However, the activity of the 5'-flanking region was suppressed by the repressor activity within the first intron. These data provide the basis for the further characterization of the regulation of the orphan GPR edg-1.
Liu
C H
CH
Department of Physiology, University of Connecticut School of Medicine, Farmington 06030, USA.
Hla
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D019204:GTP-Binding Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017781:Genes, Immediate-Early; D005865:Gestational Age; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D014018:Tissue Distribution
D017931:DNA Primers; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D004247:DNA; D019204:GTP-Binding Proteins