Poxvirus preparation CONPIND initiates production of the major inflammatory mediators IL-1 alpha and TNF-alpha in human whole blood and in blood mononuclear cell cultures.
The poxvirus preparation CONPIND initiated the production of the two major inflammatory mediators interleukin-1 alpha (IL-1 alpha) and tumour necrosis factor-alpha (TNF-alpha) in whole blood and in blood mononuclear cell cultures of man at 37 degrees C within 12-48 h. The release of the cytokines followed a dose-efficacy curve. High levels of IL1-alpha (up to 810 pg/ml) and TNF-alpha (up to 533 pg/ml) were determined by sandwich enzyme-linked immunosorbent assay.
Ahne
W
W
Veterinary Faculty of the University of Munich, Germany.
Mayr
A
A
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D001688:Biological Products / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000097:blood*; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D011212:Poxviridae / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000276:Adjuvants, Immunologic; D001688:Biological Products; D018836:Inflammation Mediators; D007375:Interleukin-1; C028621:PIND-AVI; C044867:PIND-ORF; D014409:Tumor Necrosis Factor-alpha