Guided bone regeneration in long bone. An experimental study.
OBJECTIVE
In the concept of guided tissue regeneration (GTR), a space is created for selected cells to differentiate, proliferate and repair the defect at last by implanting a membrane around the defect area, which serves as a physiologic barrier. This experiment was designed to test GTR in long bone, namely, guided bone regeneration (GBR).


METHODS
Ten New Zealand rabbits were used in this experiment. A piece of silicone membrane sutured as a tube was used to bridge a 10-mm defect on radius. 10-mm defects were also produced on the control sides. Radiography of forelimbs was taken weekly until 12 weeks. Gross sample examination, 3-point bending test and histology were involved in evaluating bone regeneration.


RESULTS
By the 12th week, seven of 10 experimental sides were healed, 2 were healed with a connective cartilage zone, and I was not healed. None of the control was healed but the defect was occupied by soft tissue.


CONCLUSIONS
The results of this preliminary study showed that GBR is present in long bone of rabbits. The following points relate to the mechanism of GBR; providing a space for bone regeneration; preventing surrounding tissue from the defect; increasing the density of osteogenic precursor cells and concentration of bone morphogenetic protein (BMPs); and maintaining a complete blood clot to bridge the fracture ends in the tube, which provides a structure for osteogenic cells ingrowth.
Lu
S
S
Department of Orthopaedics, General Hospital of Chinese PLA, Beijing.
Zhang
Z
Z
Wang
J
J
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis; D001861:Bone Regeneration; D005260:Female; D017102:Fracture Healing; D008297:Male; D011817:Rabbits; D011885:Radius Fractures / Q000378:metabolism / Q000503:physiopathology*; D011897:Random Allocation; D012826:Silicone Elastomers
D019485:Bone Morphogenetic Proteins; D012826:Silicone Elastomers