[Protective effect of lipid A monoclonal antibody against burn sepsis in rats].
A lipid A monoclonal antibody(mAb) was prepared and it was used to study its protective effect against burn sepsis. Wistar rats were inflicted with 30% TBSA third degress burn, and they were given LPS to mimic early sepsis after burn. The rats were divided randomly into burn with LPS, monoclonal antibody treatment, and control groups. The levels of endotoxin, tumor necrosis factor, light and electron microscopic studies of the morphological changes in the liver were studied. The results showed that the anti-lipid A monoclonal antibody demonstrated capacity to cross-react with several Gram-negative bacteria and their endotoxins. The mAb improved the survival rate of rats and decreased the levels of endotoxin and TNF as well as the liver damage significantly.
Xiao
H
H
Institute of Burn Research, South-western Hospital, Third Military Medical College, Chongqing.
Fu
W
W
Xiao
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002056:Burns / Q000150:complications*; D005260:Female; D008050:Lipid A / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000209:etiology / Q000517:prevention & control*
D000911:Antibodies, Monoclonal; D008050:Lipid A