Expression and epitopic conservation of calponin in different smooth muscles and during development.
Calponin is a thin filament associated protein found in smooth muscle as a potential modulator of contraction. Five mouse monoclonal antibodies (mAbs CP1, CP3, CP4, CP7, and CP8) were prepared against chicken gizzard alpha-calponin. The CP1 epitopic structure is conserved in smooth muscles across vertebrate phyla and is highly sensitive to CNBr cleavage in contrast with the chicken-specific CP4 and the avian-mammalian-specific CP8 epitopes that are resistant to CNBr fragmentation. Using this panel of mAbs against multiple epitopes, only alpha-calponin was detected in adult chicken smooth muscles and throughout development of the gizzard. Western blotting showed that the calponin content varied among different smooth muscle tissues and correlated with that of h-caldesmon. In contrast with the constitutive expression of calponin in phasic smooth muscle of the digestive tract, very low levels of calponin were detected in adult avian tracheas and no calponin expression was detected in embryonic and young chick tracheas. These results provide information on the structural conservation of calponins and suggest a relationship between calponin expression and smooth muscle functional states.
Jin
J P
JP
Department of Medical Biochemistry, University of Calgary, Canada.
Walsh
M P
MP
Resek
M E
ME
McMartin
G A
GA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D002642:Chick Embryo; D000939:Epitopes / Q000276:immunology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins; D024510:Muscle Development; D009130:Muscle, Smooth / Q000254:growth & development / Q000378:metabolism*; D009928:Organ Specificity; D013045:Species Specificity; D014714:Vertebrates / Q000276:immunology / Q000378:metabolism; D000096985:Calponins
D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D000939:Epitopes; D008840:Microfilament Proteins; D000096985:Calponins