Hepatitis G virus replication in human cultured cells displaying susceptibility to hepatitis C virus infection.
We recently developed a hepatitis C virus (HCV) replication system using two cultured human cell lines: MT-2C, a human T-cell leukemia virus type I-infected cloned T cell line, and PH5CH, a non-neoplastic human hepatocyte line. In the course of the study, we found evidence that replication of hepatitis G virus (HGV), which has recently been identified, was supported in both MT-2C and PH5CH cells. When these cells were inoculated with serum 1B-3 containing both HCV and HGV obtained from a blood donor, the 5'-noncoding (5'-NC) regions of HCV RNA and HGV RNA were detected by RT-nested PCR in both cell lines more than 30 days postinoculation, and the 3'-noncoding region of HGV RNA was also detected in the both cell lines more than 30 days postinoculation. Sequence analysis of the 5'-NC region of HGV RNA revealed the quasispecies nature of HGV, although the 5'-NC region was highly conserved among HGV isolates. This HGV-infected culture system will be useful for various biological and virological studies of HGV.
Ikeda
M
M
Virology Division, National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan.
Sugiyama
K
K
Mizutani
T
T
Tanaka
T
T
Tanaka
K
K
Shimotohno
K
K
Kato
N
N
D001483:Base Sequence; D001782:Blood Donors; D002460:Cell Line; D002999:Clone Cells; D017931:DNA Primers; D004198:Disease Susceptibility; D018067:Flaviviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016174:Hepacivirus / Q000502:physiology*; D015368:Human T-lymphotropic virus 1 / Q000502:physiology; D006801:Humans; D008099:Liver; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000737:chemistry; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes; D014779:Virus Replication
D017931:DNA Primers; D012367:RNA, Viral