[Construction of gene library of attenuated liver hepatitis A vaccine (H2 strain)].
OBJECTIVE
To determine the complete gene library of attenuated live hepatitis A vaccine (H2 strain) from cloned cDNA by application of reverse transcription (RT) and polymerase chain reaction (PCR) technique.


METHODS
Attenuated HAV H2 strain that was selected and studied in China was proven attenuated for human beings and proved to be effective in preventing hepatitis A.


RESULTS
Ten overlapping cDNA clones were obtained in addition to spanned the entire genome. The restriction enzyme mapping was also determined.


CONCLUSIONS
The gene library will benefit for the sequence of HAV cDNA and study on attenuation.
Chen
Y
Y
Zhejiang Academy of Medical Sciences, Hangzhou.
Yang
N
N
Hong
Y
Y
D004279:DNA, Viral / Q000235:genetics; D015723:Gene Library; D022362:Hepatitis A Vaccines; D017957:Hepatitis A Virus, Human / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014613:Vaccines, Attenuated; D014761:Viral Hepatitis Vaccines
D004279:DNA, Viral; D022362:Hepatitis A Vaccines; D014613:Vaccines, Attenuated; D014761:Viral Hepatitis Vaccines