title: An ADP-ribosylation-factor(ARF)-like protein involved in regulated secretion.
abstract: A rat ADP-ribosylation factor(ARF)-like protein named ARL184 was identified by cDNA cloning. The corresponding recombinant protein had an apparent molecular mass of 22,000. The deduced amino acid sequence had 55% identity with the human ARL1 and four functional GTP-binding sites. Immunofluorescent confocal microscopy studies showed that ARL184 was present in the cytosol as well as in the Golgi apparatus, raising the possibility that it has a role in a secretory pathway. The involvement of this ARF-like protein in secretion was confirmed by demonstrating that ARL184 potentiated acetylcholine release in stably transfected PC12 cells. Collectively these results suggest that this ARL protein is a component of a regulated secretory pathway involved in Ca2(+)-dependent release of acetylcholine.
authors.affiliation: Laboratoire de Génétique Moléculaire de la Neurotransmission, C.N.R.S., Hôpital de la Pitié-Salpêtrière, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Icard-Liepkalns
authors.forename: P
authors.initials: P
authors.lastname: Ravassard
authors.forename: V A
authors.initials: VA
authors.lastname: Liepkalns
authors.forename: F
authors.initials: F
authors.lastname: Chatail
authors.forename: J
authors.initials: J
authors.lastname: Mallet
mesh_terms: D020727:ADP-Ribosylation Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D016716:PC12 Cells; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; C485820:Arl2 protein, rat; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors