Effect of maternally acquired Aujeszky's disease (pseudorabies) virus-specific antibody in pigs on establishment of latency and seroconversion to differential glycoproteins after low dose challenge.
This study investigated whether (1) passively immune pigs could become latently infected after challenge with low doses of wild type pseudorabies virus (PRV) and (2) if seroconversion to PRV could be consistently detected using two commercially available differential diagnostic ELISAs. Three litters of piglets with passively acquired PRV serum neutralizing (SN) antibody (geometric mean titers 47.03 to 95.10) were challenged at 6 to 12 days of age with 236 to 500 TCID50 of Shope strain virus; pigs were vaccinated at 11 weeks of age with a commercially available genetically engineered vaccine (TK- gE- gG- Iowa S62 strain PRV). Vaccination was intended to reduce the risk of reactivation of latent infection resulting in spread of virulent PRV infection to previously uninfected pigs during the experiment. Vaccination at this age also approximated common field practices in infected herds. After 15 weeks, all challenged pigs were seropositive on the PRV glycoprotein (g or gp) E differential ELISA but were seronegative on the gG differential ELISA. All three challenge groups had pigs that were latently infected as evidenced by the detection of PRV DNA by polymerase chain reaction (PCR) assay of their trigeminal ganglia (TG). There was a significant inverse relationship observed for age at challenge and the proportion of PCR positive pigs in the group 15 weeks postchallenge (p = 0.0004). This trend was independent of the passively acquired PRV SN antibody titers at challenge. In this study, passively acquired antibody did not provide protection against establishment of latent infection in piglets after exposure to low doses of virulent PRV. These latent infections were detected serologically by only one of two available differential diagnostic ELISA.
McCaw
M B
MB
Food Animal and Equine Medicine Dept., College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA. mmccaw@sn1.cvm.ncsu.edu
Osorio
F A
FA
Wheeler
J
J
Xu
J
J
Erickson
G A
GA
D000704:Analysis of Variance; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D017931:DNA Primers; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D011558:Herpesvirus 1, Suid / Q000302:isolation & purification / Q000502:physiology*; D007112:Immunity, Maternally-Acquired; D016133:Polymerase Chain Reaction / Q000379:methods; D011557:Pseudorabies / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D013552:Swine; D017735:Virus Latency
D000914:Antibodies, Viral; D017931:DNA Primers