Tumor cell growth is inhibited by suppressing metallothionein-I synthesis.
The effect of metallothionein (MT)-I antisense oligodeoxynucleotide (ODN) on the growth of three kinds of tumor cells was studied, since MTs may be involved in cell growth. When MT-I antisense ODN was added to leukemia P388 cells, cell growth was inhibited in a manner dependent on the dose and incubation time. MT-I antisense ODN was also inhibitory for other tumor cell lines, i.e. Ehrlich carcinoma and sarcoma 180. A significant decrease in the level of MT, but not of Zn, was observed in MT-I antisense ODN-treated cells. On the other hand, control ODN did not inhibit the cell growth appreciably. These results indicate that MT-I expression may be necessary for the growth and survival of these tumor cells.
Takeda
A
A
Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Yada, Japan. takedaa@ys7.u-shizuoka-ken.ac.jp
Hisada
H
H
Okada
S
S
Mata
J E
JE
Ebadi
M
M
Iversen
P L
PL
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D007941:Leukemia P388 / Q000473:pathology; D008668:Metallothionein / Q000037:antagonists & inhibitors / Q000502:physiology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000502:physiology
D016376:Oligonucleotides, Antisense; D008668:Metallothionein; D015032:Zinc