Presence of a neutralizing domain in isolates of rubella virus in Cordoba, Argentina.
We studied the presence of a neutralizing epitope of rubella virus (RV) in locally circulating strains in Cordoba, Argentina, using binding by the monoclonal antibody (MAb) H3. This epitope is contained in a sequence of the E1 glycoprotein (E1208-239) represented by the synthetic peptide SP15. H3 MAb showed specific binding to SP15 by enzyme-linked immunosorbent assay (ELISA). One wild-type postnatal isolate, four clones derived from this isolate, and one congenital isolate were reactive with H3 by ELISA. These results suggest that the region of RV represented by SP15 is a domain present in locally circulating strains.
Cordoba
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Instituto de Virología J. M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Grutadauria
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SL
Cuffini
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C
Zapata
M T
MT
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D001118:Argentina; D001667:Binding, Competitive / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012412:Rubella virus / Q000737:chemistry / Q000276:immunology*; D014764:Viral Proteins / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D010455:Peptides; D014764:Viral Proteins