title: Plasma antioxidant vitamins, chronic hepatitis B virus infection and urinary aflatoxin B1-DNA adducts in healthy males.
abstract: Epidemiological evidence indicates that aflatoxin B1 (AFB1) intake is associated with an increased risk of hepatocellular carcinoma (HCC). The hepatocarcinogenesis is initiated by covalent binding of AFB1 to cellular DNA. To determine whether nutritional factors and hormonal status may influence the binding of AFB1 to hepatic DNA, a cross-sectional study was performed on a total of 42 male asymptomatic hepatitis B surface antigen (HBsAg) carriers and 43 male non-carriers in a cohort study on the multistage development of HCC in Taiwan. The major AFB1-DNA adduct in vivo, AFB1-N7-guanine, was measured by high-performance liquid chromatography in urine. Urinary AFB1-N7-guanine was detectable in 40% of the subjects. HBsAg carriers had a higher detection rate of urinary AFB1-DNA adducts than non-carriers and the difference was statistically significant after multivariate adjustment. After taking into account the total AFB1 urinary metabolite level, chronic HBsAg carrier status, and other potential confounders, plasma levels of cholesterol, alpha-tocopherol, and alpha- and beta-carotene were positively associated with the detection rate of the AFB1-DNA adducts in a dose-dependent manner, whereas plasma lycopene level was inversely related to the presence of the adducts in urine. The association of urinary AFB1-DNA adducts with the plasma levels of cholesterol, alpha-tocopherol, lycopene, and alpha- and beta-carotene was observed at both low and high exposure levels of AFB1. There was a synergistic interaction of plasma alpha-tocopherol with alpha- and beta-carotene on the adduct levels. No association with the adducts was found for plasma levels of retinol and testosterone. This study demonstrated different associations of antioxidant vitamins with AFB1-DNA adduct formation. The data consistent with our previous finding in cultured woodchuck hepatocytes that alpha-tocopherol and beta-carotene enhanced AFB1-DNA adduct formation suggest that prospective investigation of the relationship between plasma micronutrients and risk of AFB1-related HCC is warranted.
authors.affiliation: School of Public Health, College of Public Health, National Taiwan University, Taipei.
authors.forename: M W
authors.initials: MW
authors.lastname: Yu
authors.forename: Y C
authors.initials: YC
authors.lastname: Chiang
authors.forename: J P
authors.initials: JP
authors.lastname: Lien
authors.forename: C J
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D000328:Adult; D016604:Aflatoxin B1 / Q000031:analogs & derivatives / Q000378:metabolism / Q000633:toxicity / Q000652:urine*; D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D002338:Carotenoids / Q000097:blood; D002908:Chronic Disease; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D018736:DNA Adducts / Q000378:metabolism / Q000652:urine*; D006147:Guanine / Q000031:analogs & derivatives / Q000652:urine; D006509:Hepatitis B / Q000097:blood* / Q000652:urine*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008113:Liver Neoplasms / Q000097:blood / Q000139:chemically induced / Q000652:urine; D008297:Male; D008875:Middle Aged; D013739:Testosterone / Q000097:blood / Q000502:physiology; D014810:Vitamin E / Q000097:blood; D014815:Vitamins / Q000097:blood*; D019207:beta Carotene / Q000097:blood
chemical_list: D000975:Antioxidants; D018736:DNA Adducts; D006514:Hepatitis B Surface Antigens; D014815:Vitamins; C072611:aflatoxin B1-DNA adduct; D019207:beta Carotene; D014810:Vitamin E; D002338:Carotenoids; D013739:Testosterone; C041635:alpha-carotene; D006147:Guanine; C028319:2,3-dihydro-2-(N(7)-guanyl)-3-hydroxyaflatoxin B1; D004247:DNA; D016604:Aflatoxin B1