Thermococcus hydrothermalis sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent.
An extremely thermophilic archaeon, strain AL662T, was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at a latitude of 21 degrees N. This strain is a strictly anaerobic coccus, and its cells range from 0.8 to 2 microns in diameter. The optimum temperature, pH, and Sea Salt concentration for growth are 85 degrees C, 6, and 20 to 40 g/liter, respectively. Strain AL662T grows preferentially on proteolysis products, on a mixture of 20 amino acids, and on maltose in the presence of elemental sulfur. The membrane lipids consist of di- and tetraether glycerol lipids. The DNA G+C content is 58 mol%. Sequencing of the 16S rRNA gene showed that strain AL662T belongs to the genus Thermococcus. On the basis of hybridization results, we propose that this strain should be placed in a new species, Thermococcus hydrothermalis.
Godfroy
A
A
Département Environnement Profond, IFREMER, Centre de Brest, Plouzané, France.
Lesongeur
F
F
Raguénès
G
G
Quérellou
J
J
Antoine
E
E
Meunier
J R
JR
Guezennec
J
J
Barbier
G
G
D000143:Acids / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D002455:Cell Division; D004269:DNA, Bacterial / Q000032:analysis; D006358:Hot Temperature; D008055:Lipids / Q000032:analysis; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009792:Oceans and Seas; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012623:Seawater / Q000382:microbiology*
D000143:Acids; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D008055:Lipids; D012336:RNA, Ribosomal, 16S