title: Lack of influence of a long-term high or low vitamin E diet on the oxidative DNA damage in the bone marrow of mice.
abstract: To investigate the influence of dietary vitamin E (VE) on DNA damage in bone marrow, we fed mice either a low VE diet (-VE), a basal VE diet (+30 mg VE/kg) or a high VE diet (+1000 mg VE/kg) for 50 weeks. DNA damage was evaluated by two cytogenetic methods: micronucleus (MN) assay using peripheral blood, and examination of sister chromatid exchanges (SCE) in bone marrow cells. The MN assay was performed periodically from 6 to 50 weeks, and showed that the incidence of reticulocytes containing MNs did not increase in the low VE diet group, and did not decrease in the high VE diet group. SCE assay done at 50 weeks also showed no difference among the VE diet groups. VE in bone marrow was markedly lower in the low VE diet group and higher in the high VE diet group compared to that in the basal VE diet group at 6 weeks. The VE at 50 weeks was not markedly different from that at 6 weeks. Corresponding to the changes in the VE, lipid peroxide in bone marrow was higher in the low VE diet group, but was not lower in the high VE diet group. Glutathione and vitamin C in the bone marrow, which were about 100-1000 times higher than that of VE, were not different among the groups.
authors.affiliation: Division of Applied Food Research, National Institute of Health and Nutrition, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Umegaki
authors.forename: H
authors.initials: H
authors.lastname: Uramoto
authors.forename: T
authors.initials: T
authors.lastname: Esashi
mesh_terms: D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D001205:Ascorbic Acid / Q000032:analysis; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D001853:Bone Marrow / Q000737:chemistry / Q000378:metabolism*; D001854:Bone Marrow Cells; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects*; D004032:Diet / Q000592:standards*; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000032:analysis; D008054:Lipid Peroxides / Q000032:analysis / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D010084:Oxidation-Reduction; D012156:Reticulocytes / Q000166:cytology; D012854:Sister Chromatid Exchange; D013997:Time Factors; D014810:Vitamin E / Q000032:analysis / Q000494:pharmacology*
chemical_list: D000447:Aldehydes; D000975:Antioxidants; D008054:Lipid Peroxides; D014810:Vitamin E; D004247:DNA; D005978:Glutathione; C027576:4-hydroxy-2-nonenal; D001205:Ascorbic Acid