Elastin degradation by matrix metalloproteinases. Cleavage site specificity and mechanisms of elastolysis.
Insoluble elastin was used as a substrate to characterize the peptide bond specificities of human (HME) and mouse macrophage elastase (MME) and to compare these enzymes with other mammalian metalloproteinases and serine elastases. New amino termini detected by protein sequence analysis in insoluble elastin following proteolytic digestion reveal the P'1 residues in the carboxyl-terminal direction from the scissile bond. The relative proportion of each amino acid in this position reflects the proteolytic preference of the elastolytic enzyme. The predominant amino acids detected by protein sequence analysis following cleavage of insoluble elastin with HME, MME, and 92-kDa gelatinase were Leu, Ile, Ala, Gly, and Val. HME and MME were similar in their substrate specificity and showed a stronger preference for Leu/Ile than did the 92-kDa enzyme. Fibroblast collagenase showed no activity toward elastin. The amino acid residues detected in insoluble elastin following hydrolysis with porcine pancreatic elastase and human neutrophil elastase were predominantly Gly and Ala, with lesser amounts of Val, Phe, Ile, and Leu. There were interesting specificity differences between the two enzymes, however. For both the serine and matrix metalloproteinases, catalysis of peptide bond cleavage in insoluble elastin was characterized by temperature effects and water requirements typical of common enzyme-catalyzed reactions, even those involving soluble substrates. In contrast to what has been observed for collagen, the energy requirements for elastolysis were not extraordinary, consistent with cleavage sites in elastin being readily accessible to enzymatic attack.
Mecham
R P
RP
Department of Cell Biology and Physiology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. bmecham@cellbio.wustl.edu
Broekelmann
T J
TJ
Fliszar
C J
CJ
Shapiro
S D
SD
Welgus
H G
HG
Senior
R M
RM
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D017364:Collagenases / Q000378:metabolism*; D004549:Elastin / Q000737:chemistry* / Q000378:metabolism*; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D019272:Leukocyte Elastase / Q000378:metabolism; D008264:Macrophages / Q000201:enzymology; D008322:Mammals; D020783:Matrix Metalloproteinase 7; D008666:Metalloendopeptidases / Q000378:metabolism*; D051379:Mice; D008961:Models, Structural; D010196:Pancreatic Elastase / Q000378:metabolism*; D013379:Substrate Specificity; D013816:Thermodynamics; D014333:Tropoelastin / Q000737:chemistry / Q000378:metabolism
D014333:Tropoelastin; D004549:Elastin; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020783:Matrix Metalloproteinase 7