title: Neurone-specific enolase and Sangtec 100 assays during cardiac surgery: Part III--Dose haemolysis affect their accuracy?
abstract: Neurone-specific enolase (NSE) and Sangtec 100 (S-100) are useful for detecting cerebral damage during cardiopulmonary bypass (CPB). However, red cells contain NSE, and the haemolysis frequently caused by CPB could produce a false rise in NSE; S-100 is not found in red cells and should not be affected. We, therefore, compared the effects of haemolysis on NSE and S-100 to see if correction was necessary and possible. From seven patients, serial dilutions of haemolysed red cells were added to plasma (1/64-1/2048), measured for absorption at 540 nm and assayed for NSE and S-100. S-100 concentrations showed no change with haemolysis. Measured NSE increased significantly with haemolysis > 1/512 (an increase of 6.6 micrograms/ml): a correction formula is presented. In 39/48 patients after CPB, mean haemolysis was < 1/256 and would not need any correction. NSE and S-100 assay can, therefore, be used throughout CPB, which allows both glial and neuronal damage to be studied.
authors.affiliation: Department of Anaesthesia, Royal Postgraduate Medical School, London, UK.
authors.forename: F
authors.initials: F
authors.lastname: Gao
authors.forename: D N
authors.initials: DN
authors.lastname: Harris
authors.forename: S
authors.initials: S
authors.lastname: Sapsed-Byrne
authors.forename: S
authors.initials: S
authors.lastname: Sharp
mesh_terms: D016477:Artifacts; D002315:Cardiopulmonary Bypass; D002561:Cerebrovascular Disorders / Q000097:blood / Q000175:diagnosis*; D004912:Erythrocytes / Q000201:enzymology; D005189:False Positive Reactions; D006461:Hemolysis; D006801:Humans; D007430:Intraoperative Care; D007431:Intraoperative Complications / Q000097:blood / Q000175:diagnosis*; D009419:Nerve Tissue Proteins / Q000097:blood*; D009928:Organ Specificity; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D009418:S100 Proteins / Q000097:blood*
chemical_list: D009419:Nerve Tissue Proteins; D011933:Reagent Kits, Diagnostic; D009418:S100 Proteins; D010751:Phosphopyruvate Hydratase