Changes in the expression of voltage-gated K+ currents during development of human megakaryocytic cells.
We distinguished four distinct groups of megakaryocytic cells on the basis of their voltage-gated membrane currents. One group of 32 cells (15%), exhibited an inward rectifying current and had a diameter of 12 +/- 3.5 microm (mean +/- S.D.). A large group of 85 cells (39%) exhibited only a 'leakage-like' current and had a diameter of 15.8 +/- 3.7 microm. The other two groups of cells exhibited voltage-gated outward currents. One group consisted of 43 'I-type' cells (19%), with a diameter of 22.3 +/- 3.4 microm, for which the maximal outward current occurred for a voltage step from -60 to either 0 or +20 mV. For the last group of 60 'M-type' cells (27%), which had a diameter of 26.7 +/- 2.9 microm, the maximal outward current occurred for a voltage step from -60 to +80 mV, the largest voltage step used. The currents recorded in this study, from megakaryocytes having 'leakage-like' currents and 'I-type' currents, were indistinguishable from the voltage-gated currents of the megakaryocytes from myelogenous leukemia patients, in which voltage-gated currents were suppressed (Kapural, L., O'Rourke, F., Feinstein, M.B. and Fein, A. (1995) Blood 86, 1043), suggesting that the megakaryocytes from the myelogenous leukemia patients are a dedifferentiated or less mature form of megakaryocyte.
Kapural
L
L
Department of Physiology, University of Connecticut, Health Center, Farmington 06030, USA.
Fein
A
A
D002454:Cell Differentiation; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology*; D007951:Leukemia, Myeloid / Q000473:pathology; D008533:Megakaryocytes / Q000166:cytology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010316:Particle Size; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D012965:Sodium Chloride; D011189:Potassium Chloride