Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8-hydroxy-2'-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment.
Ratios of urinary 8-hydroxy-2'-deoxyguanosine to urinary creatinine (8-OHdG/creatinine) have been considered as a good biological indicator of DNA oxidation. Urinary 8-OHdG/creatinine levels of lung cancer patients were evaluated by enzyme-linked immunosorbent assay using a monoclonal antibody N45.1 during radiotherapy and chemotherapy. An increase in urinary 8-OHdG/creatinine was found in non-small-cell carcinoma (non-SCC) patients during the course of radiotherapy. SCC patients showed higher levels of urinary 8-OHdG/creatinine than the controls. Furthermore, SCC patients with complete or partial response to the chemotherapy showed a significant decrease in urinary 8-OHdG/creatinine while patients with no change or progressive disease showed an increase.
Erhola
M
M
Department of Respiratory Medicine, University of Tampere, Finland.
Toyokuni
S
S
Okada
K
K
Tanaka
T
T
Hiai
H
H
Ochi
H
H
Uchida
K
K
Osawa
T
T
Nieminen
M M
MM
Alho
H
H
Kellokumpu-Lehtinen
P
P
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000652:urine; D018288:Carcinoma, Small Cell / Q000188:drug therapy / Q000532:radiotherapy / Q000652:urine; D003404:Creatinine / Q000652:urine; D003430:Cross-Sectional Studies; D004247:DNA / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000532:radiotherapy / Q000652:urine*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D012907:Smoking; D016896:Treatment Outcome
D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003404:Creatinine; D003849:Deoxyguanosine