title: Effect of hormones on expression of prolactin receptor messenger ribonucleic acids in pancreatic islets of adult female mice in vitro.
abstract: We studied the effects of hormones on expression of prolactin receptor (PRL-R) mRNA in pancreatic islets of adult female mice in vitro. We quantified mRNA expression in small amounts of the islet tissue by competitive PCR and one-sided competitive PCR. Fifty pancreatic islets from adult female mice were cultured in a wall for 4 days with or without ovine prolactin (PRL), bovine growth hormone or estradiol-17 beta. PRL (1 microgram/ml) significantly increased the insulin secretion and the amount of PRL-R mRNA relative to that of beta-actin mRNA. Growth hormone (1 microgram/ml) also increased the relative amount of PRL-R mRNA, although it did not significantly increase the insulin secretion. Neither insulin secretion nor the relative amount of PRL-R mRNA was affected by estradiol (100 ng/ml). The ratio of the short form to the long form of PRL-R mRNA was not altered by these hormones. The present observation that PRL increased PRL-R mRNA expression in pancreatic islets thus suggests the possibility that PRL up-regulates the tissue sensitivity to PRL itself during lactation.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Mori
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011388:Prolactin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D011981:Receptors, Prolactin / Q000096:biosynthesis*
chemical_list: D004967:Estrogens; D012333:RNA, Messenger; D011981:Receptors, Prolactin; D011388:Prolactin; D013006:Growth Hormone