Isolation of viable egg cells of rape (Brassica napus L.).
The isolation of viable egg cells of rape (Brassica napus L.) has been achieved from microdissected ovules. The non-gametic cells of the embryo sac, synergids and central cells have also been isolated. Their morphology corresponded to that of these cells in situ, making a discrimination from isolated sporophytic cells possible. Two hours after isolation the egg cells were still viable. Viable egg cells have been reproducibly isolated with a frequency of 25% per dissected ovule.
Katoh
N
N
Japan Tobacco Inc., Oyama, Japan.
Lörz
H
H
Kranz
E
E
D001937:Brassica / Q000166:cytology*; D002469:Cell Separation; D008856:Microscopy, Fluorescence; D010063:Ovum / Q000166:cytology*; D013194:Staining and Labeling