A human gene encoding morphine modulating peptides related to NPFF and FMRFamide.
FMRFamide-related peptides have been isolated from both invertebrates and vertebrates and exhibit a wide range of biological effects in rats. We show here that in humans 2 FMRFamide-related peptides are encoded by a single gene expressed as a spliced mRNA. The larger predicted peptide (AGEGLNSQFWSLAAPQRFamide) differs from the peptide isolated from bovines (AGEGLSSPFWSLAAPQRFamide) by the substitutions of 2 amino acids. The shorter predicted peptide (NPSF, SQAFLFQPQRFamide) is 3 amino acids longer than the bovine 8 amino-acid NPFF (FLFQPQRFamide) or the human NPFF peptide isolated from serum [5], suggesting that the encoded protein is subject to cleavage by a tripeptidyl peptidase or by a novel processing mechanism. On rat spinal cord, the larger peptide is indistinguishable in activity from the equivalent bovine peptide whereas the smaller extended peptide is inactive.
Perry
S J
SJ
Sussex Centre for Neuroscience, University of Sussex, Brighton, UK.
Yi-Kung Huang
E
E
Cronk
D
D
Bagust
J
J
Sharma
R
R
Walker
R J
RJ
Wilson
S
S
Burke
J F
JF
D000200:Action Potentials / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D019835:FMRFamide; D005796:Genes; D015894:Genome, Human; D006801:Humans; D008161:Lumbosacral Region; D008969:Molecular Sequence Data; D009020:Morphine / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics* / Q000494:pharmacology; D009842:Oligopeptides / Q000235:genetics* / Q000494:pharmacology; D018847:Opioid Peptides / Q000235:genetics* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology; D013126:Spinal Nerve Roots / Q000187:drug effects / Q000502:physiology
D009479:Neuropeptides; D009842:Oligopeptides; D018847:Opioid Peptides; C105455:neuropeptide FF, Tyr(1)-N-methyl-Phe(3)-; D019835:FMRFamide; D009020:Morphine