Molecular characterization of attenuated Junin virus strains.
The Junin virus strain Candid #1 was developed as a live attenuated vaccine for Argentine haemorrhagic fever. In this paper we report the nucleotide sequences of S RNA of Candid #1 and its more virulent ancestors XJ#44 and XJ (prototype). Their relationship to Junin virus wild-type MC2 strain and other closely and distantly related arenaviruses was also examined. Comparisons of the nucleotide and amino acid sequences of N and GPC genes from Candid #1 and its progenitor strains revealed some changes that are unique to the vaccine strain. These changes could be provisionally associated with the attenuated phenotype.
Albariño
C G
CG
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Ghiringhelli
P D
PD
Posik
D M
DM
Lozano
M E
ME
Ambrosio
A M
AM
Sanchez
A
A
Romanowski
V
V
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D018051:Junin virus / Q000235:genetics*; D008969:Molecular Sequence Data; D019251:Nucleocapsid / Q000235:genetics; D012367:RNA, Viral; D017386:Sequence Homology, Amino Acid; D014613:Vaccines, Attenuated / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics*
D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines