[The essential region of CD40 cytoplasmic domain for signal transduction in WEHI231 cells].
Ligation of surface immunoglobulin M (IgM) induced cell death and this was blocked by CD40-mediated signal in a mouse B cell line, WEHI231. In order to define which region of CD40 and how CD40-mediated signal are involved in the blocking of IgM-induced cell death, we generated several human CD40 transfectants. We demonstrated that a 10-amino acid segment in the conserved cytoplasmic region of CD40-mediated rescue from IgM-induced cell death and was involved in the activation of c-Jun aminoterminal kinase (JNK). In addition, the same segment was also important for CD40-mediated activation of extracellular signal-regulated protein kinase 2 (ERK2). Moreover, tumor necrosis factor receptor-associated factor (TRAF) 2 and 3 could bind to the same segment of CD40.
Hara
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Section of Immunopathogenesis, Hokkaido University, Sapporo, Japan.
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology; D019013:CD40 Antigens / Q000502:physiology*; D006801:Humans; D007075:Immunoglobulin M / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection
D019013:CD40 Antigens; D007075:Immunoglobulin M