[Detection of poliovirus in fecal specimens by polymerase chain reaction].
Poliovirus-RNA was detected in 11 clinical fecal specimens with AFP out of 19 samples. These comparative results between NT and Mcab identifications showed a coincidence rate of 90.90%-100%. Our experiment suggested that PCR a simple, sensitive, identification type and could be used for intratypic-differentiation of poliovirus strains. It can also be applied for the detection of poliovirus contamination in the environment.
Liu
M T
MT
Guangxi Sanitary and Anti Epidemic Centre, Nanning.
Yang
H H
HH
Wang
S S
SS
D005243:Feces / Q000821:virology; D006801:Humans; D017955:Poliovirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*
D012367:RNA, Viral