Detection and isolation of nuclear haplotypes by PCR-SSCP.
SSCP (single-strand conformational polymorphism) is used widely in the field of human biomedicine, but its potential as a population genetics tool for the recovery of nuclear gene genealogies remains to be realized. We describe and illustrate a use for SSCP in the physical isolation of nuclear haplotypes that circumvents several difficulties associated with more conventional cloning procedures. The DNA sequence can be determined directly from the isolated haplotypes and used for phylogenetic inference. SSCP provides a convenient first step toward generating nuclear genealogies for population studies.
Ortí
G
G
Department of Genetics, University of Georgia, Athens 30602, USA.
Hare
M P
MP
Avise
J C
JC
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D006239:Haplotypes; D006737:Horseshoe Crabs / Q000235:genetics*; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational