The Bacillus subtilis 168 chromosome from sspE to katA.
We have cloned and sequenced a 24.5 kb region of the Bacillus subtilis 168 chromosome spanning the sspE and katA genes. The region contains a ribosomal RNA operon, rrnD, a tRNA gene set, trnD and 17 ORFs, 16 with putative ribosome-binding sites. Four of the ORFs (ORF2, ORF14, ORF16 and ORF17) match to known B. subtilis genes (sspE, thiA, senS and katA). Eight of the remaining ORF products show similarities with proteins present in the databases, including an ATP-binding transport protein, a glutamate-1-semialdehyde aminotransferase, a thiol-specific antioxidant protein, a mitomycin radical oxidase and a ferric uptake regulation protein.
Cummings
N J
NJ
Department of Food Macromolecular Science, Institute of Food Research, Earley Gate, Whiteknights Road, Reading RG6 6BZ, UK.
Connerton
I F
IF
Department of Food Macromolecular Science, Institute of Food Research, Earley Gate, Whiteknights Road, Reading RG6 6BZ, UK.
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D002374:Catalase / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics; D019751:Intramolecular Transferases; D007535:Isomerases / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D010544:Peroxidases; D054464:Peroxiredoxins; D011506:Proteins; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D012808:Sigma Factor; D014157:Transcription Factors; D012407:rRNA Operon / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D011506:Proteins; D012345:RNA, Transfer, Amino Acid-Specific; D012097:Repressor Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C068477:ferric uptake regulating proteins, bacterial; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus; D010088:Oxidoreductases; D010544:Peroxidases; D054464:Peroxiredoxins; D002374:Catalase; C090103:KATA protein, Bacillus subtilis; D007535:Isomerases; D019751:Intramolecular Transferases; C061049:glutamate-1-semialdehyde 2,1-aminomutase