Differential display cloning identifies motility-related protein (MRP1/CD9) as highly expressed in primary compared to metastatic human colon carcinoma cells.
Differential display cloning was performed to analyze genes that are differentially expressed in matched primary and metastases-derived human colon carcinoma cell lines. This led to the identification of PMA16, a gene identical to the previously cloned motility-related protein gene (MRP1/CD9). Northern and Western blot analyses of cell lines, as well as immunostaining of tissue sections from the original tumor surgical samples, confirmed that MRP1/CD9 was highly expressed at the primary site, compared to the low levels of expression in metastases. We also demonstrated that primary colon cancer cells displayed a significantly higher migration potential, compared to metastasis-derived cells. Antibodies directed against MRP1/CD9 largely prevented cell migration in vitro, but they did not influence cell adhesion. Thus, differential display cloning has allowed for the identification of MRP1/CD9, a motility-related gene product, which may regulate the metastatic phenotype of human colon cancer.
Cajot
J F
JF
Department of Experimental Pathology, Institut Suisse de Recherches Experimentales sur le Cancer, Epalinges, Switzerland.
Sordat
I
I
Silvestre
T
T
Sordat
B
B
D015703:Antigens, CD / Q000378:metabolism*; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000556:secondary; D008562:Membrane Glycoproteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010534:Peritoneal Neoplasms / Q000378:metabolism* / Q000556:secondary; D012333:RNA, Messenger / Q000378:metabolism; D060245:Tetraspanin 29; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; C554869:CD9 protein, human; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D060245:Tetraspanin 29