Characterization of the osteoclast ruffled border chloride channel and its role in bone resorption.
Bone resorption by osteoclasts requires massive transcellular acid transport, which is accomplished by the parallel action of a V-type proton pump and a chloride channel in the osteoclast ruffled border. We have studied the molecular basis for the appearance of acid transport as avian bone marrow mononuclear cells acquire a bone resorptive phenotype in vitro. We demonstrate a critical role for regulated expression of a ruffled border chloride channel as the cells become competent to resorb bone. Molecular characterization of the chloride channel shows that it is related to the renal microsomal chloride channel, p64. In planar bilayers, the ruffled border channel is a stilbene sulfonate-inhibitable, outwardly rectifying chloride channel. A mechanism by which outward rectification of the single channel chloride current could allow efficient regulation of acidification by the channel is discussed.
Schlesinger
P H
PH
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA. paul@cellbio.wustl.edu
Blair
H C
HC
Teitelbaum
S L
SL
Edwards
J C
JC
D000818:Animals; D001862:Bone Resorption; D002454:Cell Differentiation; D002645:Chickens; D018118:Chloride Channels / Q000378:metabolism* / Q000502:physiology; D004594:Electrophysiology; D005260:Female; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism; D008051:Lipid Bilayers / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008954:Models, Biological; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism*; D017494:Proton Pumps / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D018118:Chloride Channels; D008051:Lipid Bilayers; D017494:Proton Pumps; D012333:RNA, Messenger