S-allyl cysteine inhibits activation of nuclear factor kappa B in human T cells.
Reactive oxygen species are involved in signal transduction pathways leading to nuclear factor kappa B (NF-kappa B) activation which has been implicated in the regulation of gene transcription. We recently reported that a garlic compound, S-allyl cysteine (SAC), protects bovine pulmonary artery endothelial cells from oxidant injury induced by hydrogen peroxide (H2O2). In this study we determined the effects of SAC on NF-kappa B activation in human T lymphocytes (Jurkat cells) induced by tumor necrosis factor alpha (TNF- alpha) and H2O2. Activated NF-kappa B in nuclear extracts was measured by an electrophoretic mobility shift assay using 32P-labeled probe. SAC consistently exhibited a dose-dependent inhibition of NF-kappa B activation induced by both TNF-alpha and H2O2. Supershift with specific antibodies to NF-kappa B subunits confirmed that the inducible retarded bands observed in the EMSA and p65-p50 heterodimer of the NF-kappa B/Rel protein. Our data suggest that SAC may act via antioxidant mechanisms to block NF-kappa B activation in Jurkat cells.
Geng
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Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, CA 92350, USA.
Rong
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Lau
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D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001483:Base Sequence; D002417:Cattle; D003545:Cysteine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D005737:Garlic / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D019169:Jurkat Cells; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010946:Plants, Medicinal; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000633:toxicity
D000975:Antioxidants; D005609:Free Radicals; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C065299:S-allylcysteine; D006861:Hydrogen Peroxide; D003545:Cysteine