A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues.
A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.
Dalton
J P
JP
Molecular Parasitology Unit, Queensland Institute of Medical Research, Bancroft Centre, Royal Brisbane Hospital, Australia. daltonj.@ccmail.dcu.ie
Day
S R
SR
Drew
A C
AC
Brindley
P J
PJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary; D018509:DNA, Helminth; D008099:Liver / Q000469:parasitology*; D051379:Mice; D008969:Molecular Sequence Data; D010063:Ovum; D010274:Parasitology / Q000379:methods*; D012549:Schistosoma japonicum / Q000235:genetics / Q000302:isolation & purification*; D012550:Schistosoma mansoni / Q000235:genetics / Q000302:isolation & purification*; D012554:Schistosomiasis japonica / Q000469:parasitology*; D012555:Schistosomiasis mansoni / Q000469:parasitology*
D018076:DNA, Complementary; D018509:DNA, Helminth