Genomic structure and assignment of the RhoH/TTF small GTPase gene (ARHH) to 4p13 by in situ hybridization.
The RhoH/TTF (ARHH) gene encodes a new member of the Ras superfamily of small GTPases. The gene was identified by fusion to the BCL6/LAZ3 oncogene in an initially described t(3;4)(q27;p11) translocation in a non-Hodgkin's lymphoma cell line. The predicted amino acid sequence of the RhoH/TTF gene product includes Rho-like GTPase structural motifs. The RhoH/TTF gene is restrictively expressed in hematopoietic cells and tissues. Mutations in the human RAS genes have been shown previously to be tumorigenic; in the search for a potential implication of the RhoH/TTF gene in hemopoietic malignancies, we established its genomic structure. The RhoH/TTF gene spans 35 kb and contains two exons, with the second bearing the entire amino-acid-coding region. Chromosomal mapping, by FISH experiments, places the RhoH/TTF gene on the short arm of chromosome 4, band p13.
Dallery-Prudhomme
E
E
U. 124 INSERM, Institut de Recherches sur le Cancer de Lille, France.
Roumier
C
C
Denis
C
C
Preudhomme
C
C
Kerckaert
J P
JP
Galiegue-Zouitina
S
S
D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D020558:GTP Phosphohydrolases / Q000235:genetics*; D011905:Genes, ras; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D018631:ras Proteins / Q000235:genetics
D017931:DNA Primers; D020558:GTP Phosphohydrolases; D018631:ras Proteins