[Contribution of flow cytometry to allergologic diagnosis].
The technique of Flow Cytometry for activation of basophils (TAB), expressed as the marker CD63, is at present one of the pathways of research applied to drug allergy. In this work are reported the results of TAB of Pneumoallergens and Hymenoptera venoms. TAB can define better than total IgE the atopy of the subject: in effect the fluorescence of the basophils is emphasized in comparison with non-atopic subjects. This hypothesis has been confirmed by a study of three groups of subjects. With regard to drug allergy, it is important to study patients in whom the observations have been documented very objectively by clinical history and positive skin tests to the drug, compared with a negative reference to the same drug. So, TAB has been shown to be very useful in diagnosis of allergy to certain drugs, such as the Myorelaxants.
Sabbah
A
A
Laboratoire d'Immuno-Allergologie-CHU Angers.
Drouet
M
M
Sainte-Laudry
J
J
Lauret
M G
MG
Loiry
M
M
D000485:Allergens / Q000276:immunology; D000707:Anaphylaxis / Q000139:chemically induced / Q000175:diagnosis / Q000276:immunology; D000732:Androstanols / Q000009:adverse effects / Q000276:immunology; D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D001491:Basophils / Q000187:drug effects / Q000276:immunology*; D001514:Bee Venoms / Q000276:immunology; D004285:Dogs; D004342:Drug Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D004391:Dust; D005434:Flow Cytometry; D006636:Histamine Release; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D008925:Mites / Q000276:immunology; D003473:Neuromuscular Nondepolarizing Agents / Q000009:adverse effects / Q000276:immunology; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D011058:Pollen / Q000276:immunology; D000077123:Rocuronium; D012882:Skin Tests; D060149:Tetraspanin 30; D014862:Wasp Venoms / Q000276:immunology
D000485:Allergens; D000732:Androstanols; D015703:Antigens, CD; D001514:Bee Venoms; D004391:Dust; D003473:Neuromuscular Nondepolarizing Agents; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D014862:Wasp Venoms; D007073:Immunoglobulin E; D000077123:Rocuronium