Decreased expression of LFA-1 on peripheral blood lymphocytes in graft versus host disease.
We have previously demonstrated an increase in lymphocyte function associated antigen-1 (LFA-1) expression in the native intestines of animals with graft versus host disease (GVHD) after small bowel transplantation (SBTx). The present study evaluated LFA-1 expression on peripheral blood lymphocytes (PBLs) during GVHD. GVHD was created in LBNF1 rats by heterotopic vascularized SBTx from Lewis donors and compared to sham-op controls and cyclosporine-treated SBTx rats (SBTx-CyA, 10 mg/kg/day). PBLs were harvested on Postop Day 13 when signs of severe GVHD were present and PBLs were stained for LFA-1, CD3 (T-cell marker), and CD45 (B cell marker) and analyzed on an Epics 5 flow cytometer. Mesenteric lymph node (MLN) lymphocytes from the native intestines were also harvested for each group and stained for LFA-1. There were significant decreases in LFA-1, CD3, and CD45 PBL expression in rats with GVHD. CyA treatment corrected the abnormal CD3 and CD45 ratios, but not LFA-1 expression. It is concluded that: (1) The proportion of PBLs expressing LFA-1 is depressed in animals with clinical GVHD consistent with their recruitment into the host's inflamed tissues. (2) CyA treatment corrects the abnormalities in T and B cell ratios but not the decreased expression of LFA-1 on PBLs and may relate to its known imperfect ability to treat GVHD.
Poritz
L S
LS
Department of Surgery, Pennsylvania State University Medical Center, Hershey 17033, USA.
Tilberg
A F
AF
Koltun
W A
WA
D000818:Animals; D017252:CD3 Complex / Q000378:metabolism; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000378:metabolism*; D016130:Immunophenotyping; D007421:Intestine, Small / Q000276:immunology / Q000637:transplantation*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013997:Time Factors
D017252:CD3 Complex; D016169:Lymphocyte Function-Associated Antigen-1; D017493:Leukocyte Common Antigens