Direct colorimetric monoclonal antibody enzyme immunoassay for estradiol-17 beta in saliva.
We developed a direct microtiter plate enzyme immunoassay to measure estradiol-17 beta in saliva. The assay has a commercially available monoclonal antibody, raised against estradiol-17 beta-6-carboxymethyloximebovine serum albumin, and a homologous horseradish peroxidase conjugate measured colorimetrically. The detection limit (equivalent to B0-3 SD) is 365 amol/well or 7.3 pmol/L when 50-microL samples are assayed. Cross-reactivity with estrone and estriol, testosterone, or progesterone is < 0.2%. Estradiol-17 beta was measured in daily samples over five natural menstrual cycles and eight cycles stimulated as a preliminary to in vitro fertilization, and the concentrations and fluctuations found agreed with previously published data. This method gives results in approximately 3 h and may be useful for fertility monitoring and management.
Tamate
K
K
National Diagnostics Centre-Bioresearch Ireland, University College, Galway, Ireland.
Charleton
M
M
Gosling
J P
JP
Egan
D
D
Ishikawa
M
M
Fottrell
P F
PF
Kane
M M
MM
D000911:Antibodies, Monoclonal; D003124:Colorimetry; D004958:Estradiol / Q000032:analysis*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007124:Immunoenzyme Techniques; D008183:Luteal Phase; D008297:Male; D008597:Menstrual Cycle; D010060:Ovulation; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D004958:Estradiol