title: Protein involvement in the fusion between the equatorial segment of acrosome-reacted human spermatozoa and liposomes.
abstract: Artificial membranes (liposomes) can interact with the equatorial segment (ES) of human spermatozoa, provided that the acrosome reaction (AR) has occurred [Arts, Kuiken, Jager and Hoekstra (1993) Eur. J. Biochem. 217, 1001-1009]. Using fluorescently labelled liposomes, this interaction can be seen as either punctate fluorescence in the ES (lip-ESp), reflecting only bound liposomes, or as diffuse fluorescence in this region (lip-ESd), indicating that the liposomes have fused with the ES membrane. Only equatorial segments that still contain constituents of the acrosomal matrix have the capacity to bind liposomes and eventually to fuse with them. Since the exposure of such intact equatorial segments is the exclusive result of induction of the AR under physiological conditions, these results imply that liposomes can be used for the rapid detection of acrosome-reacted spermatozoa. The lip-ESp and lip-ESd patterns were shown to be reflections of two distinct properties of the ES. Proteolytic treatment after AR completely inhibited the formation of a lip-ESd pattern, whereas formation of the lip-ESp pattern was only marginally inhibited by the proteolytic treatment. The same results were obtained using anti-sperm antibodies, which did not react with acrosome-intact spermatozoa. Proteolytic treatment of spermatozoa before AR induction had no effect on the fusion capacity of the ES after subsequent AR, which implies that the putative fusion protein is not accessible before AR. Thus fusion of liposomes with the ES of human spermatozoa is mediated by a sperm protein(s), whereas the lip-ESp pattern is not likely to represent the liposome-binding stage that precedes the fusion step.
authors.affiliation: Section of Reproductive Medicine, Department of Obstetrics and Gynaecology, University Hospital Groningen, P.O. Box 30.001, NL-9700 RB Groningen, The Netherlands.
authors.forename: E G
authors.initials: EG
authors.lastname: Arts
authors.forename: J G
authors.initials: JG
authors.lastname: Wijchman
authors.forename: S
authors.initials: S
authors.lastname: Jager
authors.forename: D
authors.initials: D
authors.lastname: Hoekstra
mesh_terms: D000177:Acrosome / Q000502:physiology*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D008297:Male; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure; D015044:Zona Pellucida / Q000502:physiology*; D015053:Zygote / Q000502:physiology
chemical_list: D005456:Fluorescent Dyes; D008081:Liposomes; D008565:Membrane Proteins