title: Self-association of Band 3, the human erythrocyte anion exchanger, in detergent solution.
abstract: Dimeric Band 3 purified in n-dodecyl octaethyleneglycol (C12E8) underwent an irreversible, temperature-dependent association, resulting in a complex with a Stokes radius slightly larger than a native tetramer, before forming a higher molecular weight aggregate. Self-association occurred with a half-time of about 1 h at 37 degrees C but did not occur at 0 degrees C after several days. No change in the secondary structure of Band 3, as observed by circular dichroism, occurred during the association process. However, self-association of Band 3 was accompanied by loss of the stilbene disulfonate inhibitor binding site. No association or loss of inhibitor binding occurred with the dimeric membrane domain under similar incubation conditions. The membrane domain dimer was also stable over a wide range of pH (5.5-9.5) and buffer conditions, while Band 3 aggregated below pH 6.5. Inhibitors of anion transport, which stabilize the membrane domain, slowed the association. Band 3, depleted of phospholipids by extensive washing of resin-bound protein with detergent or, incubated with excess detergent, was more prone to aggregation. The membrane domain also showed some aggregation when depleted of lipids. Preparations could be stabilized by adding dimyristoylphosphatidylcholine (DMPC) prior to the 37 degrees C incubation. The effect of inhibitors and DMPC was additive, with a combination of 1 mM 4,4'-dinitrostilbene-2,2'-disulfonate (DNDS) and 1:1 (wt/wt) DMPC:Band 3 stabilizing 90% of the protein to a 24-h incubation at 37 degrees C. The results suggest that self-association of Band 3 dimers is promoted by the cytoplasmic domain but results in alterations to the membrane domain involving the loss of essential phospholipids. Addition of phospholipid or inhibitors to Band 3 results in a stable preparation of the intact protein that may be suitable for crystallization studies.
authors.affiliation: MRC Group in Membrane Biology, Department of Medicine, University of Toronto, Canada.
authors.forename: J W
authors.initials: JW
authors.lastname: Vince
authors.forename: V E
authors.initials: VE
authors.lastname: Sarabia
authors.forename: R A
authors.initials: RA
authors.lastname: Reithmeier
mesh_terms: D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003902:Detergents; D019281:Dimerization; D004134:Dimyristoylphosphatidylcholine / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017433:Protein Structure, Secondary; D013267:Stilbenes / Q000378:metabolism / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D003902:Detergents; D013267:Stilbenes; C006711:benzoylamido-4'-aminostilbene-2,2'-disulfonate; C022306:4,4'-dinitro-2,2'-stilbenedisulfonic acid; D004134:Dimyristoylphosphatidylcholine