title: Prolactin receptors in human meningiomas: characterization and biological role.
abstract: Sixty cerebral meningioma specimens obtained at surgery from 34 female and 26 male patients were examined for the presence of prolactin (PRL) receptors. These were compared with normal arachnoid tissue from which these tumours arise. PRL receptors were detected in 61.7% of meningiomas whereas no PRL binding was found in samples of normal arachnoid tissue. No relationship was found when sex or histological findings were compared with the presence of PRL receptors. Receptor-positive tumours had saturable and high-affinity (Kd, 4.8 +/- 0.5 ng/ml) receptors with hormonal specificity for human PRL (hPRL) resembling that of other target tissues of PRL in man. The biological role of these receptors was investigated in primary cell cultures derived from meningioma tissue characterized for PRL receptor. When human PRL was added to the culture medium, in doses ranging from 1 to 200 ng/ml, a dose-dependent stimulation of 3H-thymidine incorporation was observed only in PRL-receptor positive tumours. The PRL concentrations required to produce a half-maximal effect ranged from 11 to 20 ng/ml and were quite close to the dissociation constant (Kd) of binding of PRL to its receptors. PRL also caused an increase of cell number compared with control with a significant effect after 3 and 4 days of culture. In conclusion, these findings indicate that a large number of human meningiomas express specific and functional receptors for PRL which are involved in mediating its proliferative effects.
authors.affiliation: Department of Anatomy, Pharmacology and Forensic Medicine, Regina Margherita Children's Hospital, Turin, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Muccioli
authors.forename: C
authors.initials: C
authors.lastname: Ghè
authors.forename: G
authors.initials: G
authors.lastname: Faccani
authors.forename: M
authors.initials: M
authors.lastname: Lanotte
authors.forename: M
authors.initials: M
authors.lastname: Forni
authors.forename: E
authors.initials: E
authors.lastname: Ciccarelli
mesh_terms: D000328:Adult; D000368:Aged; D001099:Arachnoid / Q000737:chemistry; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D008579:Meningioma / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D011388:Prolactin / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D011981:Receptors, Prolactin / Q000032:analysis* / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
chemical_list: D011981:Receptors, Prolactin; D011388:Prolactin