The positional distribution of dioleoyl-palmitoyl glycerol influences lymph chylomicron transport, composition and size in rats.
The effects of 1,3-dioleoyl-2-palmitoyl glycerol (OPO) on lymph chylomicron transport, composition and size in rats were investigated in comparison with 1,2-dioleoyl-3-palmitoyl glycerol (OOP). The OPO and OOP were prepared by enzymatic transesterification reactions. The concentrations of OPO and OOP in the preparations were 65.7 g/100 g, and the composition of fatty acids was similar for each. The OPO preparation contained triacylglycerols with 76.6% of the palmitic acid in the sn-2 position, whereas 100% of the oleic acid was esterified to the sn-2 position in the OOP preparation. Rats were infused with lipid emulsion containing 150 g/L of OPO or OOP via a stomach catheter. Lymph was collected through the mesenteric lymphatic trunk at 1-h intervals for 12 h. Collected lymph chylomicrons were analyzed for triacylglycerol, fatty acids, apolipoprotein A-I and particle size. The maximum transport rates of triacylglycerols in the OPO group were higher than those in the OOP group. The overall absorption of triacylglycerols, palmitic acid and oleic acid in the OPO group was also higher than that in the OOP group. In the chylomicrons, 60-70% of the fatty acids at the sn-2 position of the infused triacylglycerol was transported at the original position. The transport rates of dioleoyl-palmitoyl glycerol in the OPO group were higher than those in the OOP group. The transport rates of apolipoprotein A-I did not differ between groups, whereas the mean diameter of the chylomicrons in the OPO group was larger than that in the OOP group. These results indicate that OPO is absorbed and transported more effectively than OOP.
Aoe
S
S
Nutritional Science Laboratory, Snow Brand Milk Products Co., Ltd., Kawagoe, Saitama 350-11, Japan.
Yamamura
J
J
Matsuyama
H
H
Hase
M
M
Shiota
M
M
Miura
S
S
D000818:Animals; D016632:Apolipoprotein A-I / Q000493:pharmacokinetics; D001692:Biological Transport; D002914:Chylomicrons / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008196:Lymph / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009829:Oleic Acids / Q000737:chemistry / Q000493:pharmacokinetics; D010169:Palmitic Acids / Q000737:chemistry / Q000493:pharmacokinetics; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000737:chemistry* / Q000378:metabolism* / Q000493:pharmacokinetics / Q000502:physiology
D016632:Apolipoprotein A-I; D002914:Chylomicrons; D005227:Fatty Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D014280:Triglycerides; C095376:1,3-dioleoyl-2-palmitoylglycerol; C106818:1,2-dioleoyl-3-palmitoylglycerol