Monoclonal anti-fosB antibody specific for predetermined, nonstructural region of the fosB protein.
Comparison of the primary structures and theoretical prediction of the potential antigenic determinant of the deduced Fos proteins reveals the presence of a nonstructural and hydrophilic region juxtaposed to the leucine zipper and nonconserved among the Fos protein family. To develop monoclonal anti-peptide antibodies capable of distinguishing all Fos-proteins, synthetic peptides specific for the mentioned predicted region were synthesized manually by the "tea-bag" method. Immunization of Balb/c mice with fosB-related synthetic peptide BSA gave rise to mouse hybridoma cell line K21 (IgG1, kappa) secreting highly specific antibodies against corresponding human fosB protein. Fine mapping of the MAb K21 indicated that the minimal epitope essential for the recognition is the sequence GPGPLAE.
Kilcevska
K
K
Department of Genetic Engineering, University of Latvia, Riga.
Jackevica
L
L
Steinbergs
J
J
Gusars
I
I
Sallberg
M
M
Tsimanis
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D004352:Drug Resistance, Microbial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D016760:Proto-Oncogene Proteins c-fos / Q000276:immunology*
D010446:Peptide Fragments; D016760:Proto-Oncogene Proteins c-fos