Isolation and identification of chicken infectious anemia virus in China.
A chicken infectious anemia virus (CIAV) isolate was obtained from broiler flocks aged 25-40 days with anemia and poor performance and was designated SR43. The CIAV isolate was resistant to treatment with chloroform and induced thymus atrophy, bone marrow aplasia, and low hematocrit values when inoculated into 1-day-old, susceptible, specific-pathogen-free chicks. CIAV-specific antigens could be demonstrated in SR43-infected MDCC-MSB-1 cells, a cell line derived from a Marek's disease lymphoma, with the use of a monoclonal antibody specific for CIAV. CIAV DNA in infected MDCC-MSB-1 cell cultures was detected by using a polymerase chain reaction assay. These findings demonstrate that CIAV is present in China.
Zhou
W
W
Institute of Animal Husbandry and Veterinary Sciences, Beijing Academy of Agricultural and Forestry Sciences, People's Republic of China.
Shen
B
B
Yang
B
B
Han
S
S
Wei
L
L
Xiao
B
B
Zhou
J
J
D000818:Animals; D001284:Atrophy; D001853:Bone Marrow / Q000473:pathology; D002460:Cell Line; D017727:Chicken anemia virus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens; D002681:China; D002725:Chloroform / Q000494:pharmacology; D018173:Circoviridae Infections / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D008380:Marek Disease; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases; D015183:Restriction Mapping; D013950:Thymus Gland / Q000473:pathology; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D004279:DNA, Viral; D002725:Chloroform