Glycogenolysis stimulation by non-steroidal anti-inflammatories in the perfused rat liver is not accompanied by Ca2+ release.
Several non-steroidal anti-inflammatories increase glycogenolysis and are also able to affect intracellular Ca2+ channels. In the perfused liver, glycogenolysis stimulation is often associated with a transient Ca2+ efflux, which occurs immediately after the introduction of the glycogenolytic agonist. The purpose of this work was to verify if the introduction of non-steroidal anti-inflammatories also stimulates Ca2+ efflux. Rat livers were pre-loaded with 45Ca2+ in a recirculating system. The anti-inflammatory drugs diclofenac and niflumate were infused 10 minutes after restoration of the once-through perfusion. Glycogenolysis and oxygen uptake stimulation caused by these compounds was not accompanied by any increment in 45Ca2+ release. Subsequent infusion of vasopressin greatly stimulated 45Ca2+ efflux, denoting that the intracellular Ca2+ pools had not been depleted. These results corroborate previous results suggesting that Ca2+ is not involved in glycogenolysis stimulation caused by non-steroidal anti-inflammatories.
Nascimento
E A
EA
Department of Biochemistry, University of Maringá, Brazil.
Tonon
F A
FA
Kelmer-Bracht
A M
AM
Bracht
A
A
Ishii-Iwamoto
E L
EL
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D004008:Diclofenac / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009544:Niflumic Acid / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014667:Vasopressins / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D014667:Vasopressins; D004008:Diclofenac; D009544:Niflumic Acid; D019297:2,4-Dinitrophenol; D010100:Oxygen; D002118:Calcium