Nedd5, a mammalian septin, is a novel cytoskeletal component interacting with actin-based structures.
The mouse Nedd5 gene encodes a 41.5-kD GTPase similar to the Saccharomyces and Drosophila septins essential for cytokinesis. Nedd5 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody. Microinjection of anti-Nedd5 antibody interferes with cytokinesis, giving rise to binucleated cells. In interphase and postmitotic cells, Nedd5 localizes to fibrous or granular structures depending on the growth state of the cell. The Nedd5-containing fibers are disrupted by microinjection of GTPgammaS and by Nedd5 mutants lacking GTP-binding activity, implying that GTP hydrolysis is required for its assembly. The Nedd5-containing fibers also appear to physically contact actin bundles and focal adhesion complexes and are disrupted by cytochalasin D, C3 exoenzyme, and serum starvation, suggesting a functional interaction with the actin-based cytoskeletal systems in interphase cells.
Kinoshita
M
M
Department of Molecular Oncology, Graduate School of Medicine, Kyoto University, Japan. mkinoshi@virus.kyoto-u.ac.jp
Kumar
S
S
Mizoguchi
A
A
Ide
C
C
Kinoshita
A
A
Haraguchi
T
T
Hiraoka
Y
Y
Noda
M
M
D016475:3T3 Cells; D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D004330:Drosophila; D019143:Evolution, Molecular; D020558:GTP Phosphohydrolases / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D015723:Gene Library; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology; D058112:Septins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D000199:Actins; D003598:Cytoskeletal Proteins; D011993:Recombinant Fusion Proteins; D020558:GTP Phosphohydrolases; C106863:Sept2 protein, mouse; D058112:Septins