Analysis of the nucleolar organizing regions in the ant Tapinoma nigerrimum (Hymenoptera, Formicidae).
This study analyses the NORs of Tapinoma nigerrimum, a species that, as known from previous studies, has various chromosomes which carry a NOR site. The analysis was made by a combination of three methods: silver nitrate staining, in situ hybridization with fluorescein-or digoxigenin-labelled probes, and staining with the CG-specific fluorochrome chromomycin A3. The silver staining technique showed an Ag-positive region on chromosome 6 and on various other chromosomes. However, the application of in situ hybridization techniques showed only one positive signal in the proximal region of the short arm of chromosome 6 of T. nigerrimum. Similar results were observed by CMA banding. The absence of rDNA genes or the presence of only a small number of these, not detectable with the above probes, might explain the absence of hybridization signal in the remaining chromosomes.
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Departamento de Biologia Experimental, Universidad de Jaén, Spain.
Aránega
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Luque
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Palomeque
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D000818:Animals; D001000:Ants / Q000235:genetics*; D014128:Chromomycin A3 / Q000737:chemistry; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000235:genetics; D004076:Digoxigenin / Q000737:chemistry; D004171:Diploidy; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins / Q000737:chemistry; D017344:Genes, Insect; D006238:Haploidy; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D009697:Nucleolus Organizer Region / Q000235:genetics*; D012835:Silver Nitrate / Q000737:chemistry; D016622:Silver Staining
D015342:DNA Probes; D004275:DNA, Ribosomal; D005452:Fluoresceins; D012835:Silver Nitrate; D014128:Chromomycin A3; D004076:Digoxigenin; D019793:Fluorescein