[The study of the relationship between the granule size of immunoliposomes and the conjugate ratio of F(ab')2 of McAb HI30 (CD45)].
The immunoliposomes were prepared by conjugating the F(ab')2 of monoclonal antibodies HI30(CD45) labelled by 125I with the liposomes labelled by 131I, and then were separated by a sephacryl S-300 column. The chromatography showed that the liposomes prepared by ultrasonic method were not homogeneous, and the liposomes of smaller granule size had a higher conjugate ratio. It was also found that the fragment F(ab')2 of McAb conjugated to liposomes could enlarge their granule size.
Liu
J
J
Institute of Hematology, CAMS, Tianjin.
Liu
H
H
Chen
Z
Z
Li
S
S
Shen
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008081:Liposomes; D010316:Particle Size; D051381:Rats
D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D008081:Liposomes; D017493:Leukocyte Common Antigens