title: Promoter sequences from two different Brassica napus tapetal oleosin-like genes direct tapetal expression of beta-glucuronidase in transgenic Brassica plants.
abstract: To investigate the sequences responsible for the regulated expression of tapetal-specific oleosin-like genes, ca. 2 kb of the 5'-upstream regions from two divergent genes, OlnB;4 and OlnB;13, were isolated, sequenced and fused to the reporter gene beta-glucuronidase for study in transgenic Brassica napus plants. Although the proteins encoded by these two genes are highly divergent, except for the conserved oleosin-like domain, the first 250 bp of their 5'-upstream regions was 86% identical, including a region of 150 bp upstream from the TATA box. Analysis of 42 independent transformants by histochemical and fluorometric methods showed that both promoters directed tapetal-specific expression that peaked at the 4 mm flower bud stage.
authors.affiliation: Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, Canada.
authors.forename: H P
authors.initials: HP
authors.lastname: Hong
authors.forename: J H
authors.initials: JH
authors.lastname: Ross
authors.forename: J L
authors.initials: JL
authors.lastname: Gerster
authors.forename: S
authors.initials: S
authors.lastname: Rigas
authors.forename: R S
authors.initials: RS
authors.lastname: Datla
authors.forename: P
authors.initials: P
authors.lastname: Hatzopoulos
authors.forename: G
authors.initials: G
authors.lastname: Scoles
authors.forename: W
authors.initials: W
authors.lastname: Keller
authors.forename: D J
authors.initials: DJ
authors.lastname: Murphy
authors.forename: L S
authors.initials: LS
authors.lastname: Robert
mesh_terms: D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D014644:Genetic Variation; D005966:Glucuronidase / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011994:Recombinant Proteins; D005966:Glucuronidase