title: Fas ligand expression in glioblastoma cell lines and primary astrocytic brain tumors.
abstract: Fas/APO-1 (CD95) is a cell surface receptor that mediates apoptosis when it reacts with Fas ligand (FasL) or Fas antibody. We previously reported that Fas expression is predominantly induced in perinecrotic glioma cells, suggesting that Fas induction is associated with apoptosis and necrosis formation, a histological hallmark of glioblastomas. In this study, we assessed the expression of FasL in 10 glioblastoma cell lines and in 14 astrocytic brain tumors (three low-grade astrocytomas and 11 glioblastomas). Reverse transcriptase (RT)-PCR revealed that all glioblastoma cell lines and primary astrocytic brain tumors express FasL. Immunohistochemically, FasL was predominantly expressed on the plasma membrane of glioma cells. These results suggest that FasL expression is common in human astrocytic brain tumors and may cause apoptosis of glioma cells if Fas expression is induced.
authors.affiliation: International Agency for Research on Cancer, France.
authors.forename: C
authors.initials: C
authors.lastname: Gratas
authors.forename: Y
authors.initials: Y
authors.lastname: Tohma
authors.forename: E G
authors.initials: EG
authors.lastname: Van Meir
authors.forename: M
authors.initials: M
authors.lastname: Klein
authors.forename: M
authors.initials: M
authors.lastname: Tenan
authors.forename: N
authors.initials: N
authors.lastname: Ishii
authors.forename: O
authors.initials: O
authors.lastname: Tachibana
authors.forename: P
authors.initials: P
authors.lastname: Kleihues
authors.forename: H
authors.initials: H
authors.lastname: Ohgaki
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D017209:Apoptosis; D001254:Astrocytoma / Q000276:immunology* / Q000473:pathology; D001932:Brain Neoplasms / Q000276:immunology* / Q000473:pathology; D002648:Child; D053222:Fas Ligand Protein; D005260:Female; D005909:Glioblastoma / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008024:Ligands; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000096:biosynthesis
chemical_list: D000951:Antigens, Neoplasm; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008024:Ligands; D008562:Membrane Glycoproteins; D019014:fas Receptor