title: T lymphocyte proliferative capability to defined stimuli and costimulatory CD28 pathway is not impaired in healthy centenarians.
abstract: It is generally assumed that T cell proliferation is impaired in aged individuals. We report data on the proliferative capability of peripheral blood mononuclear cells (PBMC) and T lymphocytes from 40 healthy people of different ages, (19-107 years), including 14 centenarians, to defined mitogenic stimuli. We observed no age-related proliferative impairment both in PBMC and in purified T cells stimulated by anti-CD3 mAb or phorbol myristate acetate (PMA). Furthermore, T cells stimulated by anti-CD3 mAb or PMA and costimulated by CD28 mAb did not proliferate differently among young, middle aged subjects and centenarians. Thus, short term T cell proliferation is not affected even at extreme age when well defined stimuli are used on cells deriving from carefully selected healthy subjects.
authors.affiliation: Istituto di Clinica Medica Generale e Terapia Medica, University of Parma, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Sansoni
authors.forename: F
authors.initials: F
authors.lastname: Fagnoni
authors.forename: R
authors.initials: R
authors.lastname: Vescovini
authors.forename: M
authors.initials: M
authors.lastname: Mazzola
authors.forename: V
authors.initials: V
authors.lastname: Brianti
authors.forename: G
authors.initials: G
authors.lastname: Bologna
authors.forename: E
authors.initials: E
authors.lastname: Nigro
authors.forename: G
authors.initials: G
authors.lastname: Lavagetto
authors.forename: A
authors.initials: A
authors.lastname: Cossarizza
authors.forename: D
authors.initials: D
authors.lastname: Monti
authors.forename: C
authors.initials: C
authors.lastname: Franceschi
authors.forename: M
authors.initials: M
authors.lastname: Passeri
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000276:immunology*; D018106:CD28 Antigens / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002469:Cell Separation; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology*; D012016:Reference Values; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
chemical_list: D018106:CD28 Antigens; D008934:Mitogens