Post-SELEX combinatorial optimization of aptamers.
In vitro selection techniques provide a means of isolating nucleic acid ligands for binding to particular protein targets. Although most aptamers have quite high affinities for their target proteins, it has been shown that post-SELEX modification can result in further enhancement of binding affinity, as well as other desired properties. This has led to the current development of a more systematic approach to aptamer optimization using a combinatorial screening methodology.
Eaton
B E
BE
NeXstar Pharmaceuticals, Inc., Boulder, CO 80301, USA.
Gold
L
L
Hicke
B J
BJ
Janjić
N
N
Jucker
F M
FM
Sebesta
D P
DP
Tarasow
T M
TM
Willis
M C
MC
Zichi
D A
DA
D001483:Base Sequence; D007256:Information Systems; D008024:Ligands; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000138:chemical synthesis / Q000378:metabolism; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship
D008024:Ligands; D009841:Oligonucleotides; D011506:Proteins; D012313:RNA