title: Specificity of amylases and cyclodextrin-glucanotransferase in reactions with 2-deoxy-maltooligosaccharides.
abstract: 2-Deoxy-maltooligosaccharides of different chain length were tested as substrates for exo- and endo-amylases. Cleavage occurred with beta-amylase, yielding 2,2'-dideoxy-maltose, and with amyloglucosidase. With the alpha-amylase from Thermomonospora curvata tris-(2-deoxy)-maltotriose and the corresponding tetra- and pentasaccharides were formed. Porcine pancreatic alpha-amylase did not tolerate the deoxygenated substrate, nor were cyclization experiments with cyclodextrin-glucanotransferase (CGT) successful. In a coupling reaction with CGT, however, a series of transfer products to the acceptor 2-deoxyglucose were obtained.
authors.affiliation: Institut für Organische Chemie, Universität Hamburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Evers
authors.forename: M
authors.initials: M
authors.lastname: Petríćek
authors.forename: J
authors.initials: J
authors.lastname: Thiem
mesh_terms: D000681:Amylases / Q000737:chemistry*; D005964:Glucosyltransferases / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D009844:Oligosaccharides; C021705:maltooligosaccharides; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D000681:Amylases