title: Solution structure of human CTLA-4 and delineation of a CD80/CD86 binding site conserved in CD28.
abstract: The structure of human CTLA-4 reveals that residues Met 99, Tyr 100 and Tyr 104 of the M99YPPPY104 motif are adjacent to a patch of charged surface residues on the A'GFCC' face of the protein. Mutation of these residues, which are conserved in the CTLA-4/CD28 family, significantly reduces binding to CD80 and/or CD86, implicating this patch as a ligand binding site.
authors.forename: W J
authors.initials: WJ
authors.lastname: Metzler
authors.forename: J
authors.initials: J
authors.lastname: Bajorath
authors.forename: W
authors.initials: W
authors.lastname: Fenderson
authors.forename: S Y
authors.initials: SY
authors.lastname: Shaw
authors.forename: K L
authors.initials: KL
authors.lastname: Constantine
authors.forename: J
authors.initials: J
authors.lastname: Naemura
authors.forename: G
authors.initials: G
authors.lastname: Leytze
authors.forename: R J
authors.initials: RJ
authors.lastname: Peach
authors.forename: T B
authors.initials: TB
authors.lastname: Lavoie
authors.forename: L
authors.initials: L
authors.lastname: Mueller
authors.forename: P S
authors.initials: PS
authors.lastname: Linsley
mesh_terms: D000069594:Abatacept; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018122:B7-1 Antigen / Q000378:metabolism*; D051940:B7-2 Antigen; D001665:Binding Sites; D018106:CD28 Antigens / Q000378:metabolism*; D060908:CTLA-4 Antigen; D017124:Conserved Sequence; D019281:Dimerization; D006801:Humans; D018796:Immunoconjugates; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D012996:Solutions; D013440:Sulfides
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D018106:CD28 Antigens; C497964:CD86 protein, human; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D018796:Immunoconjugates; D008562:Membrane Glycoproteins; D012996:Solutions; D013440:Sulfides; D000069594:Abatacept