Glucose metabolism and protective biochemical mechanisms in a rat brain affected by kaolin-induced hydrocephalus.
To clarify glucose metabolism in a hydrocephalic rat brain, substances related to glycolytic metabolism were biochemically measured. Kaolin-induced hydrocephalic rats were sacrificed and lactate dehydrogenase (LDH), LDH isozyme, lactate, adenosine triphosphate (ATP), and isocitrate dehydrogenase (ICDH) were measured in the following regions: cortex, thalamus, midbrain, hippocampus, cerebellum, and pons with medulla. During the development of hydrocephalus, lactate and LDH increased in most regions, the LDH M-subunit increased in the cortex, and ICDH decreased in most regions. However, ATP levels did not change. The increases in lactate, LDH and M-subunit suggested an anaerobic environment in the cell leading to activation of the anaerobic glycolysis. The decrease in ICDH represented a diminution of the tricarboxylic acid cycle. Through these alterations, the ATP level can be kept constant during the course of hydrocephalus, allowing the brain to create a better biochemical milieu.
Hidaka
M
M
Department of Neurosurgery, Tokai University School of Medicine, Kanagawa, Japan.
Matsumae
M
M
Yamamura
M
M
Tsugane
R
R
Sato
O
O
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001921:Brain / Q000473:pathology / Q000503:physiopathology*; D001931:Brain Mapping; D004579:Electron Transport / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology*; D006019:Glycolysis / Q000502:physiology; D006849:Hydrocephalus / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D007521:Isocitrate Dehydrogenase / Q000502:physiology; D007527:Isoenzymes; D007616:Kaolin; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D051381:Rats
D001786:Blood Glucose; D007527:Isoenzymes; D007616:Kaolin; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase