Expression of estrogen receptor-beta in human breast tumors.
The expression of a recently described novel estrogen receptor, ER-beta, was detected in several human breast tumor biopsy samples and several human breast epithelial cell lines using reverse transcription and polymerase chain reaction (RT-PCR) analysis. Cloning and sequencing of the PCR product from a breast tumor confirmed the identity of the sequence with that of the ER-beta mRNA previously reported in human testis. The expression of ER-beta was not correlated with that of ER-alpha, and both ER-alpha positive and ER-alpha negative cell lines expressed ER-beta mRNA. However, some breast tumors and some cell lines coexpress ER-beta and ER-alpha mRNA. Our data support a possible role for ER-beta in human breast cancer.
Dotzlaw
H
H
Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.
Leygue
E
E
Watson
P H
PH
Murphy
L C
LC
D001706:Biopsy; D001943:Breast Neoplasms / Q000378:metabolism*; D002478:Cells, Cultured; D047629:Estrogen Receptor beta; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D014407:Tumor Cells, Cultured
D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D011960:Receptors, Estrogen