title: Distribution of heat shock proteins in eutopic and ectopic endometrium in endometriosis and adenomyosis.
abstract: OBJECTIVE
  To evaluate the pathophysiologic role of heat shock proteins and to examine the effect of danazol on these proteins in patients with endometriosis and adenomyosis.
  
  
  METHODS
  Immunohistochemical identification of human heat shock proteins 27, 60, and 70 in endometrial glandular cells identified using monoclonal antibodies.
  
  
  METHODS
  Department of obstetrics and gynecology in a university hospital.
  
  
  METHODS
  Subjects were 119 women with documented endometriosis or adenomyosis. The subjects were divided into three groups: fertile control (n = 38), with 14 in the proliferative phase and 24 in the secretory phase; endometriosis (n = 38); and adenomyosis (n = 43), including 33 who underwent hysterectomy and 10 treated with danazol.
  
  
  METHODS
  Staining of glandular cells by semiquantitative immunostaining (evaluation nomogram) score.
  
  
  RESULTS
  Significantly increased expression of heat shock protein 27 was noted in eutopic endometrium from patients with endometriosis and adenomyosis as compared with controls, regardless of the menstrual phase. The scores for heat shock protein 27 and heat shock protein 70 in the ectopic endometrium of patients with endometriosis were low compared with those in eutopic endometrium, whereas in adenomyosis, the scores were similar to those of eutopic endometrium. After treatment with danazol, the expression of heat shock proteins returned to control levels.
  
  
  CONCLUSIONS
  We suggest that abnormally increased expression of heat shock proteins plays a role in the pathophysiology of endometriosis and adenomyosis.
authors.affiliation: Department of Obstetrics and Gynecology, Akita University, School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ota
authors.forename: S
authors.initials: S
authors.lastname: Igarashi
authors.forename: J
authors.initials: J
authors.lastname: Hatazawa
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D018834:Chaperonin 60 / Q000032:analysis / Q000276:immunology; D015331:Cohort Studies; D003613:Danazol / Q000627:therapeutic use; D004715:Endometriosis / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D004717:Endometrium / Q000276:immunology / Q000473:pathology*; D004965:Estrogen Antagonists / Q000627:therapeutic use; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000276:immunology; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007044:Hysterectomy; D007150:Immunohistochemistry; D051379:Mice; D008875:Middle Aged
chemical_list: D000911:Antibodies, Monoclonal; D018834:Chaperonin 60; D004965:Estrogen Antagonists; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D003613:Danazol