Human endothelial culture supernatant selectively promotes IgM-producing hybridomas.
We compared the effect of human endothelial cell culture supernatant (HECS), ESG (Ewing sarcoma growth factor), IL-6 (interleukin-6) and peritoneal macrophages on the recovery of hybridoma cells after fusion with respect to growth, stability and distribution of isotype variants. A selective growth of murine IgM-producing hybridoma cells was observed in the presence of HECS after cell fusion.
Greferath
R
R
Center for Blood Research Laboratories, Harvard Medical School, Boston, MA 02135, USA.
Sauer-Nehls
S
S
Dyballa
S
S
Nehls
P
P
D000818:Animals; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015850:Interleukin-6