Increased levels of 4-hydroxynonenal modified proteins in plasma of children with autoimmune diseases.
Analysis of serum samples of healthy children (n = 11) and children with Systemic Lupus Erythematosus (SLE), (n = 21) was performed by SDS-PAGE and immunoblot with an antibody directed against proteins modified by lipid peroxidation (LPO) product 4-hydroxynonenal (HNE). A single major stained protein band was detected. By comparison of the molecular weights in nonreducing and reducing SDS-PAGE was found that the main protein modified by HNE is immunoglobulin G. Significantly higher concentrations of the aldehyde modified protein were found in children with high disease activity of SLE measured by SLE disease activity index (SLEDAI). Lipid peroxidation measured by malondialdehyde and 4-hydroxynonenal concentrations show an enhanced level of both compounds also in patients with the active autoimmune disease. Therefore, it can be assumed that free radical mediated processes play a pathophysiological role in the active phase of SLE and HNE-modified serum proteins are a further parameter for the detection of in vivo LPO.
Grune
T
T
Clinic of Physical Medicine and Rehabilitation, Medical Faculty (Charité), Humboldt University Berlin, Germany.
Michel
P
P
Sitte
N
N
Eggert
W
W
Albrecht-Nebe
H
H
Esterbauer
H
H
Siems
W G
WG
D000293:Adolescent; D000447:Aldehydes / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015227:Lipid Peroxidation; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood
D000447:Aldehydes; D001798:Blood Proteins; D008315:Malondialdehyde; C027576:4-hydroxy-2-nonenal