Thyrotropin-releasing hormone gene expression by anterior pituitary cells in long-term cultures is influenced by the culture conditions and cell-to-cell interactions.
It has been suggested that TRH, synthesized by anterior pituitary (AP) cells in long-term monolayer cultures, may act as a paracrine or autocrine regulator. Because local control through messenger molecules depends on the cellular microenvironment, we were interested in studying the synthesis of TRH by AP cells in different culture systems and under various conditions. When AP cells were cultured as monolayers in medium containing 10% FCS for long periods of time (up to 3 weeks), a considerable increase in TRH content and prepro-TRHmessengerRNA (preproTRHmRNA) levels could be demonstrated by RIA and Northern blot analysis, whereas the cellular content of the TRH-like peptide pyroGlu-Glu-Pro-NH2 decreased with time in culture to undetectable levels. The release of TRH could be stimulated by depolarizing concentrations of K+ (55 mM), by the Ca++ ionophore A23187, and by GnRH, but not by CRH or GRF, indicating that TRH is stored in gonadotropes. Moreover, a combined in situ hybridization and immunocytochemical analysis demonstrated colocalization of LH in preproTRHmRNA-positive AP cells. When AP cells were cultured as reaggregates in the same (FCS-containing) medium, only a marginal increase in TRH content and preproTRHmRNA levels was observed. Irrespective of the culture systems and the culture conditions used, TRH gene expression was not observed when FCS was omitted. These results indicate that TRH gene expression more likely reflects derepression, rather than induction, of the TRH gene.
Peters
A
A
Max-Planck-Institut für experimentelle Endokrinologie, Hannover, Germany.
Heuer
H
H
Schomburg
L
L
De Greef
W J
WJ
Visser
T J
TJ
Bauer
K
K
D000818:Animals; D015152:Blotting, Northern; D002449:Cell Aggregation; D002450:Cell Communication / Q000235:genetics*; D002478:Cells, Cultured; D003470:Culture Media; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013973:Thyrotropin-Releasing Hormone / Q000235:genetics*; D013997:Time Factors; D014018:Tissue Distribution
D003470:Culture Media; D011498:Protein Precursors; D012333:RNA, Messenger; C063596:prepro-thyrotropin-releasing hormone; D013973:Thyrotropin-Releasing Hormone