Extensive maternal representation of DNA-binding proteins that interact with regulatory target sites of the Strongylocentrotus purpuratus CyIIIa gene.
Nine different embryonic transcription factors interact at specific target sites in the cis-regulatory domain of the Strongylocentrotus purpuratus CyIIIa gene. We tested eight of these site sequences and show here that for every one a DNA-binding protein was present in unfertilized egg cytoplasm. The concentrations of active DNA-binding proteins per egg were estimated. We also present a new and convenient method for preparation of a material cytoplasmic fraction that retains these factors in active form.
Calzone
F J
FJ
Division of Biology, California Institute of Technology, Pasadena 91125, U.S.A.
Grainger
J
J
Coffman
J A
JA
Davidson
E H
EH
D000199:Actins / Q000235:genetics*; D000818:Animals; D001755:Blastocyst / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D010063:Ovum / Q000737:chemistry*; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D012617:Sea Urchins / Q000196:embryology / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*
D000199:Actins; D004268:DNA-Binding Proteins; D014157:Transcription Factors