Identification of a minimal promoter element of the mouse epidermal growth factor gene.
We have previously generated a transgenic mouse line (EGF/Tag) in which simian virus 40 (SV40) T-antigen expression is directed by the mouse epidermal growth factor (EGF) gene promoter. In these mice, cellular hyperproliferation is observed in the submaxillary gland associated with SV40 T-antigen expression. In addition, SV40 T-antigen-expressing tumours of prostatic origin are seen. We have now derived immortalized cell lines from these tissues and have used the cells to perform a functional analysis of the EGF gene promoter. Cells were transfected with EGF promoter/reporter constructs, and an element located between 51 and 35 bases upstream of the EGF mRNA start site required for basal activity of the promoter was identified. Electrophoretic mobility-shift analysis suggests that three proteins bind to this region, one of which is either Sp1 or a closely related protein.
Pascall
J C
JC
Growth Factor Laboratory, The Babraham Institute, Cambridge CB2 4AT, U.K.
Brown
K D
KD
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D011467:Prostate / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000378:metabolism
D016329:Sp1 Transcription Factor; D004815:Epidermal Growth Factor