[Promotion of c-fos oncogene expression in cultured rabbit lens epithelial cells by macrophages].
OBJECTIVE
This study was designed to test the hypothesis that inflammatory cells deposited on the surface of implanted intraocular lens stimulate the proliferation of residual lens epithelial cells.


METHODS
Immunocytochemical ABC methods with purified anti-human Fos protein serum antibody was used to stain the cultured rabbit lens epithelial cells incubated with rabbit macrophages and macrophage-conditioned medium (MCM).


RESULTS
The lens epithelial cell growth was faster in the cultures with macrophages and MCM than in the controls. Fos protein positive staining was found in the nuclei of epithelial cells cultured with macrophages for 4 hours, and of the cells with MCM for one hour; whereas the staining was negative in the cells of control cultures.


CONCLUSIONS
Macrophages promote c-fos oncogene expression in the lens epithelial cells, that might be mediated by bioactive factors secreted by macrophages.
Hu
M
M
Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi'an.
Hao
Y
Y
Hui
Y
Y
D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells; D005260:Female; D016762:Genes, fos; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D007910:Lenses, Intraocular / Q000009:adverse effects; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D011817:Rabbits
D016760:Proto-Oncogene Proteins c-fos