Survival of human epidermal keratinocytes after short-duration high temperature: synthesis of HSP70 and IL-8.
Thermal injury by short pulses (1-30 s) of relatively high temperature (50-68 degrees C) was investigated in normal human epidermal keratinocytes (NHEK). NHEK were cultured on plastic cover-slips and dipped in medium held at various temperatures. Survival assessed by methylthiazol tetrazolium reduction assay at 6 days postheating demonstrated an inverse time-temperature relationship that indicated that most cells could survive after a 1-s, 60 degrees C exposure or a 30-s, 55 degrees C exposure. Arrhenius plots of the data indicated major transition points for cell injury at 50 and 60 degrees C. Heat shock protein 70 (HSP70) and interleukin-8 (IL-8) were both induced by elevation of temperature between 50 and 60 degrees C for as short a time as 1 s. HSP70 synthesis stimulated by short, high pulses of heat appeared to induce thermotolerance. These results demonstrate that brief exposure to relatively high temperature can induce HSP70 and IL-8 synthesis in keratinocytes.
Bowman
P D
PD
United States Army Institute of Surgical Research, Fort Sam, Houston 78234-6315, USA.
Schuschereba
S T
ST
Lawlor
D F
DF
Gilligan
G R
GR
Mata
J R
JR
DeBaere
D R
DR
D002470:Cell Survival; D002478:Cells, Cultured; D004396:Coloring Agents; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology*; D007700:Kinetics; D016462:Mammaplasty; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
D004396:Coloring Agents; D018840:HSP70 Heat-Shock Proteins; D016209:Interleukin-8; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue