Fibronectin concentrations correlate with ovarian follicular size and estradiol values in equine follicular fluid.
The amounts of total protein, albumin, fibronectin, alpha 2-macroglobulin (alpha 2-M), immunoglobulin G, ceruloplasmin and antithrombin were determined in fluids collected from 53 preovulatory equine follicles and compared with the contents of estradiol-17 beta, progesterone and androstenedione, with follicle size and the amounts of the equivalent proteins in normal equine plasma. The concentration of fibronectin and the fibronectin/albumin ratios increased significantly with follicle size and with follicular estradiol levels. The alpha 2-M levels and alpha 2-M/albumin ratios correlated with follicle size but not with hormone content. Both fibronectin and alpha 2-M were present in lower amounts in follicular fluid compared with plasma while the other proteins were present in similar amounts. Among the proteins evaluated, there was a positive correlation between the amount of the protein in the follicular fluid and the molecular weight of the protein.
Gentry
P A
PA
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Canada.
Zareie
M
M
Liptrap
R M
RM
D000418:Albumins / Q000032:analysis / Q000737:chemistry; D000735:Androstenedione / Q000032:analysis; D000818:Animals; D000990:Antithrombin III / Q000032:analysis / Q000737:chemistry; D002570:Ceruloplasmin / Q000032:analysis / Q000737:chemistry; D004958:Estradiol / Q000032:analysis*; D005260:Female; D005353:Fibronectins / Q000032:analysis* / Q000737:chemistry; D015571:Follicular Fluid / Q000737:chemistry*; D006736:Horses / Q000502:physiology*; D007074:Immunoglobulin G / Q000032:analysis / Q000737:chemistry; D016014:Linear Models; D008970:Molecular Weight; D006080:Ovarian Follicle / Q000502:physiology*; D011374:Progesterone / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis / Q000737:chemistry
D000418:Albumins; D005353:Fibronectins; D007074:Immunoglobulin G; D000511:alpha-Macroglobulins; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D000990:Antithrombin III; D002570:Ceruloplasmin