Cloning and characterization of the mouse tob gene.
The human Tob protein was identified as a molecule that interacted with the ErbB-2 protein, a receptor-type protein tyrosine kinase (RPTK). The interaction suggests that Tob is involved in RPTK-mediated signaling. In the present paper, we report molecular cloning and characterization of the mouse tob gene. The mouse tob gene contains an open reading frame of 1089 bp with 87% identity to its human counterpart in the nucleotide sequence. The coding region of mouse tob as well as human tob is not interrupted by introns. The mouse tob transcript is 2.3 kb long, the size being similar to that of the human tob transcript, and is detected ubiquitously in various tissues. Like human Tob, mouse Tob is characterized by the presence of a sequence rich in proline and glutamine which is often present in the sequence of transcription factors. In addition, the ATTTA motif characteristic of the immediate early gene is present in the 3'-untranslated region of the mouse tob gene. This, together with the conserved sequence and expression pattern of tob between mouse and human, suggests that Tob plays an important role in the response to extracellular signals.
Yoshida
Y
Y
Department of Oncology, The Institute of Medical Science, University of Tokyo, Minato-ku, Japan.
Matsuda
S
S
Yamamoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D016678:Genome; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins
D002352:Carrier Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; C098669:TOB1 protein, human; C490903:Tob1 protein, mouse; D025521:Tumor Suppressor Proteins