Failure of lymphopoiesis after adoptive transfer of NF-kappaB-deficient fetal liver cells.
Mice deficient in the p65 subunit of NF-kappaB die during fetal development. Introduction of p50/p65-deficient fetal liver cells into lethally irradiated hosts resulted in a severe deficit of fetal liver-derived lymphocytes and their immediate precursors but an overabundance of fetal liver-derived granulocytes. Surprisingly, simultaneous transplantation of wild-type bone marrow cells rescued the production of p50/p65-deficient lymphocytes. Expression of immunoglobulin K light chains on these rescued NF-kappaB-deficient B lymphocytes was normal. These results suggest that while p50 and p65 do not regulate the maturation of pre-B cells, NF-kappaB mediates the development or survival of an early lymphocyte precursor through regulation of an extracellular factor.
Horwitz
B H
BH
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Scott
M L
ML
Cherry
S R
SR
Bronson
R T
RT
Baltimore
D
D
D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005434:Flow Cytometry; D015322:Gene Rearrangement, B-Lymphocyte; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D008099:Liver / Q000196:embryology; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000172:deficiency*; D052002:NF-kappa B p50 Subunit; D011828:Radiation Chimera; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology; D051996:Transcription Factor RelA
D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D051996:Transcription Factor RelA