title: Is laminin-1 a guidance cue for cerebellar granule cell migration?
abstract: Laminin-1 is a glycoprotein found in the basement membrane of many tissues. In the cerebellum of rodents, it has also been localized along Bergmann glial fibers, where it is thought to be involved in promoting granule cell migration by enhancing adhesion and neurite outgrowth along these fibers. Recent reports, however, indicate that laminin-1 is not present on Bergmann fibers, but instead is associated with blood vessels and meninges. Furthermore, attempts to block granule cell migration using antibodies against laminin-1 have yielded conflicting results. In this report, we provide further evidence that laminin-1 is associated exclusively with blood vessels and meninges in the cerebellum of postnatal rats. In addition, we show that adhesion and neurite outgrowth of granule cells was impeded on laminin-coated surfaces. In fact, cerebellar cells dramatically and consistently avoided laminin-1 regions of patterned surfaces. Cells did adhere to laminin regions if it was coadsorbed with polylysine or tested in serum-containing medium. Avoidance of laminin-1 regions in culture was not, however, blocked by pretreatment with laminin-1 antibodies. By comparison, mouse neuroblastoma cells adhered preferentially to laminin-1 regions in serum-free medium, a response which was blocked by laminin-1 antibodies. These results indicate that laminin-1 is not involved in granule cell migration along Bergmann glial fibers. Instead, they suggest that laminin-1 may function as a repulsive guidance cue preventing granule cells from following inappropriate pathways during development.
authors.affiliation: Institute for Neuroscience and Department of Physiology, Northwestern University Medical School, Chicago, Illinois 60611-3008, USA.
authors.forename: B
authors.initials: B
authors.lastname: Lom
authors.forename: P E
authors.initials: PE
authors.lastname: Hockberger
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D002448:Cell Adhesion; D002465:Cell Movement; D002531:Cerebellum / Q000166:cytology / Q000502:physiology*; D016895:Culture Media, Serum-Free; D007797:Laminin / Q000032:analysis* / Q000502:physiology*; D051379:Mice; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011107:Polylysine; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
chemical_list: D016895:Culture Media, Serum-Free; D007797:Laminin; D011107:Polylysine