title: Experimental infection of young specific pathogen-free cats with Bartonella henselae.
abstract: Eighteen 12-week-old specific pathogen-free cats, blood culture- and serum antibody-negative for Bartonella henselae, were randomly allocated to groups and were intravenously inoculated with 10(10) (group 1), 10(8) (group 2), or 10(6) (group 3) B. henselae or with saline (group 4) or were not inoculated (group 5). Cats were humanely killed at 2, 4, 8, 16, and 32 weeks after inoculation. All B. henselae-inoculated cats were bacteremic by 2 weeks after infection. Bacteremia persisted until 32 weeks after infection in 1 cat. Cats in groups 1 and 2 had fever (>39.7 degrees C) and partial anorexia by 2 weeks after infection that lasted 2-7 days. All infected cats had Bartonella-specific IgM and IgG serum antibodies and lymphocyte blastogenic responses. Histopathologic lesions were observed in multiple organs of infected cats through 8 weeks after infection. Cats were readily infected with B. henselae by intravenous inoculation, developed histopathologic lesions that apparently resolved, and developed B and T lymphocyte responses to infection.
authors.affiliation: Department of Veterinary Pathobiology, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: L
authors.initials: L
authors.lastname: Guptill
authors.forename: L
authors.initials: L
authors.lastname: Slater
authors.forename: C C
authors.initials: CC
authors.lastname: Wu
authors.forename: T L
authors.initials: TL
authors.lastname: Lin
authors.forename: L T
authors.initials: LT
authors.lastname: Glickman
authors.forename: D F
authors.initials: DF
authors.lastname: Welch
authors.forename: H
authors.initials: H
authors.lastname: HogenEsch
mesh_terms: D000818:Animals; D018416:Bartonella henselae; D002372:Cat-Scratch Disease / Q000276:immunology* / Q000473:pathology; D002415:Cats; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D008297:Male; D013047:Specific Pathogen-Free Organisms