Immortalization of neuro-endocrine cells from adrenal tumors arising in SV40 T-transgenic mice.
Pheochromocytomas are adrenal medullary tumors which arise from the transformation of neural crest-derived cells. In the course of studies of mice transgenic for an SV40 T-gene ectopically expressed in the adrenal medulla, we observed the occurrence of large, mainly bilateral tumors in a high proportion of transgenic animals. From these tumors we established immortalized cell lines which grow in vitro at 32 degrees C (the permissive temperature for the tsA58 T-protein encoded by the transgene), but not at 38 C. These cells demonstrate characteristics of both neuronal (160 kd neurofilament) and endocrine (chromogranins) cells. The expression of Mash-1 and ret supports their initial characterization as early bipotential neuro-endocrine progenitors.
Cairns
L A
LA
Dip. di Genetica e di Biologia dei Microrganismi, Università di Milano, Italy.
Crotta
S
S
Minuzzo
M
M
Ricciardi-Castagnoli
P
P
Pozzi
L
L
Ottolenghi
S
S
D000310:Adrenal Gland Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D000313:Adrenal Medulla / Q000473:pathology; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics; D050981:Erythroid-Specific DNA-Binding Factors; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D009154:Mutation; D009414:Nerve Growth Factors / Q000494:pharmacology; D009432:Neural Crest / Q000166:cytology / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics; D014407:Tumor Cells, Cultured
D000952:Antigens, Polyomavirus Transforming; D053379:Chromogranin A; D002864:Chromogranins; D016207:Cytokines; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D009414:Nerve Growth Factors; D014157:Transcription Factors; D005346:Fibroblast Growth Factors