Intracellular distribution and characteristics of Ca(2+)-transporting systems in cells of ciliate protozoan Tetrahymena pyriformis.
A significant digitonin-sensitive calcium pool has been shown to exist in Tetrahymena pyriformis cells. In the presence of exogenic energy sources, calcium released from the digitonin-sensitive pool can be accumulated by mitochondria or endoplasmic reticulum. The kinetic characteristics of the mitochondrial and reticulum systems of Ca2+ transport and their sensitivity to various inhibitors were studied.
Yurkov
I S
IS
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region.
Zinchenko
V P
VP
Makarov
P R
PR
Kuznetsova
S M
SM
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism* / Q000493:pharmacokinetics; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004072:Digitonin / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D016808:Tetrahymena thermophila / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D002135:Calcium-Binding Proteins; D000255:Adenosine Triphosphate; D008274:Magnesium; D004072:Digitonin; D010100:Oxygen; D002118:Calcium