Hyperlipidemic nephropathy induced by adriamycin: effect of melatonin administration.
The effect of melatonin (MEL) on the nephropathy and the oxidative stress induced by a single and high dose of Adriamycin (AD) has been studied in Wistar male rats. MEL (50 microg/kg/day) was injected intraperitoneally 3 and 7 days, respectively, before and after AD injection (20 mg/kg i.p.). Trunk blood was drawn and triglycerides, total cholesterol, phospholipids, high-density lipoprotein cholesterol, urea, creatinine, total protein, lipoperoxides, and reduced glutathione (GSH) levels and catalase activity (CAT) were determined in serum. In kidney homogenates, lipoperoxides, GSH, and CAT were measured as well as total protein in urine. AD administration resulted in hyperlipidemia and high-grade proteinuria and a marked increase in serum lipoperoxides, urea, and creatinine. In the kidney, the increase in lipoperoxides was accompanied by a significant decrease of GSH and CAT. The efficiency of MEL was specially remarkable in restoring GSH, CAT, and proteinuria to the levels of controls. These results confirm the involvement of free radicals in the pathogenesis of nephrotoxicity induced by AD. Likewise, they show the high antioxidative power of MEL and its marked effect on the prevention and suppression of this nephropathy.
Montilla
P
P
Department of Biochemistry and Molecular Biology, University of Córdoba School of Medicine, Spain.
Túnez
I
I
Muñoz
M C
MC
López
A
A
Soria
J V
JV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002374:Catalase / Q000097:blood / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D004317:Doxorubicin / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D006949:Hyperlipidemias / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D008054:Lipid Peroxides / Q000097:blood / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D011507:Proteinuria / Q000139:chemically induced / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014508:Urea / Q000097:blood
D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D008054:Lipid Peroxides; D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D004317:Doxorubicin; D014508:Urea; D003404:Creatinine; D002374:Catalase; D005978:Glutathione; D008550:Melatonin