title: Immunoglobulin heavy chain diversity genes rearrangement pattern indicates that MALT-type gastric lymphoma B cells have undergone an antigen selection process.
abstract: Gastric MALT lymphoma usually develops from chronic gastritis, the vast majority of which (>90%) is associated with Helicobacter pylori infection. We sequenced the third complementarity determining region (CDR3) of immunoglobulin heavy chain genes in 19 gastric MALT lymphoma clones to determine the pattern of variable (V), diversity (D) and joining (J) gene utilization during immunoglobulin gene rearrangement. DNA was extracted from paraffin-embedded sections and the rearranged CDR3 regions were amplified using a semi-nested polymerase chain reaction (with primers complementary to the conserved framework-three segment of the variable region [FR3A] and J regions). The DNA used for cloning and sequencing was obtained after purification of monoclonal bands excised from polyacrylamide gels. The N-D-N region specific to each clone was compared with known germline D sequences. Similarly to that observed in normal and leukaemic B cells, our series of gastric MALT lymphomas showed apparent preferential utilization of genes from the DXP family. In two cases no similarity between the CDR3 nucleotide sequences of the neoplastic clones and the known germline D sequences could be found. In 10/19 analysed alleles the lymphoma B-cell clones appeared to contain two D gene segments (D-D recombination), a rare occurrence in normal individuals but one which has been described as a significant event in the determination of idiotype expression and antigen-binding affinity. Remarkably, despite the use of different D and J segments, the resultant amino acid sequences matched in two patients, suggesting the presence of a common selecting antigen. The observed pattern of D gene rearrangement suggests that MALT lymphoma B-cell clones have undergone antigen selection, which seems to indicate that the antigen stimulation plays a pivotal role in the development of the lymphoma.
authors.affiliation: Servizio Oncologico Cantonale, Ospedale San Giovanni, Bellinzona, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Bertoni
authors.forename: G
authors.initials: G
authors.lastname: Cazzaniga
authors.forename: G
authors.initials: G
authors.lastname: Bosshard
authors.forename: E
authors.initials: E
authors.lastname: Roggero
authors.forename: R
authors.initials: R
authors.lastname: Barbazza
authors.forename: M
authors.initials: M
authors.lastname: De Boni
authors.forename: C
authors.initials: C
authors.lastname: Capella
authors.forename: E
authors.initials: E
authors.lastname: Pedrinis
authors.forename: F
authors.initials: F
authors.lastname: Cavalli
authors.forename: A
authors.initials: A
authors.lastname: Biondi
authors.forename: E
authors.initials: E
authors.lastname: Zucca
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D002999:Clone Cells; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D008969:Molecular Sequence Data; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D000951:Antigens, Neoplasm; D007135:Immunoglobulin Variable Region