title: Indirect fluorescent IgM antibody response of pigs infected with porcine reproductive and respiratory syndrome syndrome virus.
abstract: IgG and IgM antibody responses were examined by an indirect fluorescent antibody method in pigs following inoculation with different porcine reproductive and respiratory syndrome virus (PRRSV) isolates or a vaccine virus. Viremia was also examined in the pigs. The IgG antibody was first detected between 9 and 14 days post inoculation (PI) and maintained high titers for at least 7 weeks PI. No change in IgG antibody titers was observed when the pigs were reinoculated with PRRSV 35 days PI. IgM antibody was detected between 5 and 28 days PI in the pigs. Reinoculation at 35 days PI caused a short term rise of IgM antibody. Virus was isolated from sera collected between 2 and 21 days PI. The IgM antibody was detected regularly in sera collected during viremia and up to 1-2 weeks after the viremic periods. These results suggest that pigs with detectable IgM antibody are probably pigs with recent infection and that routine testing of IgM antibody in purchased breeding pigs from seropositive farms may be useful in identification of pigs with recent infection.
authors.affiliation: College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
authors.forename: H S
authors.initials: HS
authors.lastname: Joo
authors.forename: B K
authors.initials: BK
authors.lastname: Park
authors.forename: S A
authors.initials: SA
authors.lastname: Dee
authors.forename: C
authors.initials: C
authors.lastname: Pijoan
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D019084:Fluorescent Antibody Technique, Indirect; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine; D013997:Time Factors; D014766:Viremia / Q000097:blood / Q000175:diagnosis / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M