An Escherichia coli host strain useful for efficient overproduction of cloned gene products with NaCl as the inducer.
Salt-induced overexpression of genes cloned downstream of the phage T7 phi10 promoter was demonstrated in an Escherichia coli strain (GJ1158) which carries a single chromosomally integrated copy of the gene for phage T7 RNA polymerase under transcriptional control of the cis-regulatory elements of the osmoresponsive proU operon. Plasmids that have been constructed to obtain overproduction of individual target gene products in strain BL21(DE3) (by addition of isopropyl-beta-D-thiogalactopyranoside as an inducer) can directly be transformed into GJ1158. The NaCl induction regimen was also shown to be associated with a decreased propensity for sequestration of overexpressed target proteins within insoluble inclusion bodies.
Bhandari
P
P
Centre for Cellular and Molecular Biology, Hyderabad, India.
Gowrishankar
J
J
D026905:Amino Acid Transport Systems; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D009876:Operon; D010455:Peptides / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*; D012995:Solubility; D014764:Viral Proteins
D026905:Amino Acid Transport Systems; D001426:Bacterial Proteins; D002352:Carrier Proteins; D010455:Peptides; C060774:ProU protein, Bacteria; D014764:Viral Proteins; D012965:Sodium Chloride; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases