Protective immunity induced by vaccination with Onchocerca volvulus tropomyosin in rodents.
A cDNA clone of Onchocerca volvulus, designated MOv14, and encoding 136 amino-acid residues from the C-terminus of O. volvulus tropomyosin, was evaluated as a protective immunogen in two complimentary rodent models of onchocerciasis. Vaccination of BALB/c mice with the recombinant fusion of MOv14 coupled to Maltose-Binding Protein (MBP) induced significant reductions (48-62%) in the recovery of Onchocerca lienalis microfilariae from the skin, compared to control groups immunized with MBP alone. The predominant antibody response generated to MOv14 by vaccination was of IgG1. Following a similar vaccination protocol in Mongolian jirds, two independent experiments demonstrated that 16 weeks after infection with Acanthocheilonema viteae there was a 46% reduction in the recovery of adult worms in vaccinated animals compared to control groups. Antibodies generated by vaccination recognized a product released during culture of A. viteae infective larvae which migrated at a distinct molecular mass from native tropomyosin from somatic tissues.
Taylor
M J
MJ
Liverpool School of Tropical Medicine, UK.
Jenkins
R E
RE
Bianco
A E
AE
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology; D002417:Cattle; D005849:Gerbillinae; D007074:Immunoglobulin G / Q000096:biosynthesis; D008297:Male; D058619:Maltose-Binding Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008842:Microfilariae / Q000276:immunology / Q000302:isolation & purification; D009854:Onchocerca / Q000276:immunology / Q000302:isolation & purification; D017181:Onchocerca volvulus / Q000235:genetics / Q000276:immunology*; D009855:Onchocerciasis / Q000276:immunology / Q000469:parasitology; D014335:Tropomyosin / Q000235:genetics / Q000276:immunology*; D014611:Vaccination
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002352:Carrier Proteins; D007074:Immunoglobulin G; D058619:Maltose-Binding Proteins; D014335:Tropomyosin