Isolation of a Dictyostelium discoideum 14-3-3 homologue.
A 1.0 kb cDNA clone (Dd14-3-3) encoding a 14-3-3 homologue was isolated from a Dictyostelium discoideum cDNA library. The putative Dd14-3-3 protein has highest sequence identity to a barley 14-3-3 isoform (74%). Southern blot analysis suggests that only one 14-3-3 gene is present in the Dictyostelium genome. Highest Dd14-3-3 expression is observed in vegetatively growing cells, and expression decreases during multicellular development. In contrast, Dd14-3-3 protein levels detected immunochemically remained constant during Dictyostelium development. Expression of the Dd14-3-3 cDNA in Saccharomyces cerevisiae complemented the lethal disruption of the two yeast genes encoding 14-3-3 proteins (BMH1 and BMH2). This shows that Dd14-3-3 can fulfil the same function(s) as the yeast 14-3-3 proteins.
Knetsch
M L
ML
Section of Cell Biology, Institute of Molecular Plant Sciences, Leiden University, The Netherlands.
van Heusden
G P
GP
Ennis
H L
HL
Shaw
D R
DR
Epskamp
S J
SJ
Snaar-Jagalska
B E
BE
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004023:Dictyostelium / Q000235:genetics* / Q000254:growth & development; D015870:Gene Expression; D001467:Hordeum / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017385:Sequence Homology; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; D014446:Tyrosine 3-Monooxygenase