Optimization of the performance of the polymerase chain reaction in silicon-based microstructures.
We have demonstrated the ability to perform real-time homogeneous, sequence specific detection of PCR products in silicon microstructures. Optimal design/ processing result in equivalent performance (yield and specificity) for high surface-to-volume silicon structures as compared to larger volume reactions in polypropylene tubes. Amplifications in volumes as small as 0.5 microl and thermal cycling times reduced as much as 5-fold from that of conventional systems have been demonstrated for the microstructures.
Taylor
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TB
PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA.
Winn-Deen
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ES
Picozza
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Woudenberg
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TM
Albin
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D000199:Actins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D012825:Silicon
D000199:Actins; D012825:Silicon