title: The role of insulin-like growth factor-I (IGF-I) and estradiol in rabbit corpus luteum progesterone production.
abstract: To determine whether insulin-like growth factor-I (IGF-I) plays a role in rabbit corpus luteum (CL) physiology the authors examined: IGF-I expression, the effect of IGF-I on progesterone (P) production in vitro, and the interaction of IGF-I with estradiol, the primary luteotropin in the rabbit. Northern blot analysis revealed that IGF-I mRNA is present in the rabbit CL throughout pseudopregnanacy. An intact ovarian in vitro perfusion model and dispersed luteal cell culture were used to determine effects of IGF-I on P production and interactions with estradiol. IGF-I significantly stimulated P production compared to control medium by the isolated, intact perfused rabbit ovary and by dispersed, cultured luteal cells. Estradiol alone did not stimulate P production in vitro. Estradiol did augment IGF-I stimulated P production in the intact perfused ovary and in luteal cell culture. These findings support a role for IGF-I in rabbit CL P production.
authors.affiliation: Department of Gynecology and Obstetrics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: S H
authors.initials: SH
authors.lastname: Chen
authors.forename: V
authors.initials: V
authors.lastname: Zanagnolo
authors.forename: S
authors.initials: S
authors.lastname: Preutthipan
authors.forename: K P
authors.initials: KP
authors.lastname: Roberts
authors.forename: S B
authors.initials: SB
authors.lastname: Goodman
authors.forename: A M
authors.initials: AM
authors.lastname: Dharmarajan
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism*; D004958:Estradiol / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000502:physiology*; D010053:Ovary / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*; D011555:Pseudopregnancy / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D011863:Radioimmunoassay
chemical_list: D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I