A transcript map encompassing the multiple endocrine neoplasia type-1 (MEN1) locus on chromosome 11q13.
A transcription map of a 1200-kb region encompassing the MEN1 locus was constructed by direct cDNA selection and mapping ESTs. A total of 29 genes were mapped. Ten transcripts were identified by cDNA selection of a focused 300-kb genomic region telomeric to the MEN1 consensus region. Since many of the sequences cloned by cDNA selection also identified ESTs from the region, 19 additional RH-mapped ESTs were mapped to the entire contig region by PCR amplification of genomic clones. Nine known genes, 2 putative human homologues to mouse genes, and 18 novel transcripts map to the region. Transcripts that map to the MEN1 interval PYGM-D11S449 include SGC35223, IB1256, AA147620, ZFM1, FAU, and CAPN1. The latter 3 known genes have already been excluded as candidate MEN1 genes. The 2 putative human homologues of mouse genes Ltbp2 and Spa-1 may be candidate tumor suppressor genes, but they map telomeric to D11S449. Although both of these genes map outside the MEN1 consensus region they may play a role in sporadic endocrine tumors independent of the MEN1 gene or in other tumors, such as breast cancer, that have loss of heterozygosity within this region.
Sawicki
M
M
Department of Surgery Molecular Biology Core Unit, West Los Angeles Veterans Administration Medical Center, California 90073, USA. msawicki@ucla.edu
Arnold
E
E
Ebrahimi
S
S
Duell
T
T
Jin
S
S
Wood
T
T
Chakrabarti
R
R
Peters
J
J
Wan
Y
Y
Samara
G
G
Weier
H U
HU
Udar
N
N
Passaro
E
E
Srivatsan
E S
ES
D015152:Blotting, Northern; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018761:Multiple Endocrine Neoplasia Type 1 / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
C105373:MEN1 protein, human; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins