Isolation and analysis of cellular DNA fragments directly binding to c-Myc protein.
c-Myc protein, the product of cellular oncogene c-myc, is thought to play an important role in the control of cell cycle progression by binding to the E-box sequence (CACGTG) of cellular DNA, but only a few target genes are known. We cloned two small human DNA fragments (n16 and r37) that bound to c-Myc protein in vitro by random screening. Both clones contained the E-box sequence, to which c-Myc protein bound directly in vitro. Northern blot analysis showed that a low molecular-weight RNA was transcribed from the region near the n16 c-Myc binding site. The function of this low molecular-weight RNA and the regulatory role of c-Myc protein in related transcription are now under investigation.
Ishida
S
S
Department of Gene Research, Cancer Institute (JFCR), Tokyo, Japan.
Takada
S
S
Koike
K
K
D001483:Base Sequence; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular; D002453:Cell Cycle; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016271:Proto-Oncogene Proteins c-myc / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D009838:Oligodeoxyribonucleotides; D016271:Proto-Oncogene Proteins c-myc