title: Immunohistochemical localization of alpha 1-acid glycoprotein in liver tissues of bovine fetuses, newborn calves, and sick or healthy adult cattle.
abstract: OBJECTIVE
  To detect localization of alpha 1-acid glycoprotein (alpha 1-AG) antigens in the liver tissue of cattle by use of immunoperoxidase technique.
  
  
  METHODS
  Liver specimens from 6 bovine fetuses, 2 healthy bovine neonates, 2 healthy adult cattle, 3 cattle with experimentally induced hepatic abscesses, and 2 cattle with enzootic bovine leukosis (EBL).
  
  
  METHODS
  3 cattle (with hepatic abscesses) were inoculated with a suspension of Fusobacterium necrophorum in the ruminal vein. Serum alpha 1-AG concentration was determined by use of the single radial immunodiffusion method. Livers from fetuses, newborn calves, and adult or sick cattle were fixed in buffered 10% formalin, dehydrated in alcohol, embedded in paraffin, sectioned, and stained by use of the avidinbiotin complex/immunoperoxidase technique.
  
  
  RESULTS
  Sites of localization of the alpha 1-AG antigen positive reaction (AGPR) in the liver obtained from bovine fetuses, neonates, or sick cattle were different. In fetal and newborn calves, the AGPR was detected in the cytoplasm of hepatocytes. Intensity of the reaction varied in direct proportion to alpha 1-AG serum concentration. In adult cattle, the AGPR was particularly intense in hepatocytes adjacent to abscesses or EBL-induced tumors.
  
  
  CONCLUSIONS
  The pattern of distribution of cells with AGPR in the liver varied, depending on severity of inflammation. In the cattle with EBL, whether the AGPR was attributable to inflammation could not be clarified, although suppression of immunologic response to tumors may have been a cause of the observed reaction. This association suggests that the glycoprotein may be synthesized, mainly in hepatocytes.
authors.affiliation: Department of Veterinary Surgery, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: Y
authors.initials: Y
authors.lastname: Motoi
authors.forename: M
authors.initials: M
authors.lastname: Haritani
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Tamura
authors.forename: K
authors.initials: K
authors.lastname: Takase
authors.forename: S
authors.initials: S
authors.lastname: Oikawa
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology / Q000378:metabolism; D002417:Cattle / Q000276:immunology / Q000378:metabolism*; D002418:Cattle Diseases / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000276:immunology / Q000378:metabolism; D005673:Fusobacterium; D005674:Fusobacterium Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary; D008099:Liver / Q000737:chemistry* / Q000276:immunology; D008100:Liver Abscess / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D009961:Orosomucoid / Q000276:immunology*
chemical_list: D009961:Orosomucoid