Clinical application of a new stain to detect acrosome-reacted sperm for predicting polyspermic fertilization in IVF-ET.
The aim of this study was to evaluate the efficacy of Blutstan staining of human spermatozoa for predicting the spermatozoa capacity of fertilization in human IVF. Blutstan, a prestained glass slide coated with dyes, is able to identify activated sperm quickly and easily. Acrosomal reactivity of spermatozoa was evaluated with this slide glass at the insemination of IVF of 30 couples. There was significant correlation between the Blutstan reactivity and the fertilization rate (r = .52, p < .01). Furthermore, spermatozoa with high Blutstan reactivity were fertilized oocytes polyspermy. This method was rapid, simple, and useful for detecting activated sperm and predicting for the polyspermic fertilization in clinical setting.
Fujino
Y
Y
Department of Obstetrics and Gynecology, Osaka City University Medical School, Japan.
Ozaki
K
K
Nakamura
Y
Y
Sun
T T
TT
Ueda
K
K
Ozaki
A
A
Ito
F
F
Ogita
S
S
D000177:Acrosome / Q000502:physiology*; D000328:Adult; D048588:Benzoxazines; D004396:Coloring Agents / Q000737:chemistry*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D008751:Methylene Blue / Q000737:chemistry*; D008875:Middle Aged; D010078:Oxazines / Q000737:chemistry*; D013094:Spermatozoa / Q000502:physiology
D048588:Benzoxazines; D004396:Coloring Agents; D010078:Oxazines; C028911:cresyl violet; D008751:Methylene Blue