Increased tissue resistance in the nude mouse against Candida albicans without altering strain-dependent differences in susceptibility.
Strain differences in tissue responses to infection with Candida albicans were examined in nude mice having susceptible (CBA/CaH) and resistant (BALB/c) parentage. Homozygous (nu/nu) mice of both strains were more resistant to systemic infection with C. albicans than heterozygous (nul+) littermates as indicated by a reduction in both the severity of tissue damage and colony counts in the brain and kidney. However, the tissue lesions in nu/nu CBA/CaH mice were markedly more severe than those in nu/nu mice with the BALB/c background. This pattern was reflected in the greater fungal burden in the CBA/CaH strain. Analysis of cDNA from infected tissues using a competitive polymerase chain reaction excluded interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha), and interleukin 6 (IL-6) as mediators of the enhanced resistance of the nude mice. The results confirm that the different patterns of lesion severity in BALB/c and CBA/CaH mice do not involve T lymphocyte-mediated pathology, and are consistent with the hypothesis that strain-dependent tissue damage is not dependent on the effector function of macrophages or their precursors.
Fulurija
A
A
Department of Pathology, University of Western Australia, Nedlands, Australia.
Ashman
R B
RB
Papadimitriou
J M
JM
D000818:Animals; D001921:Brain / Q000382:microbiology / Q000473:pathology; D002176:Candida albicans / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis; D004198:Disease Susceptibility; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007113:Immunity, Innate; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D013045:Species Specificity
D016207:Cytokines; D012333:RNA, Messenger