Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine.
Characterization of the dnmZ, dnmU, and dnmV genes from the daunorubicin-producer Streptomyces peucetius by DNA sequence analysis indicated that these genes encode a protein of unknown function plus a putative thymidine diphospho-4-keto-6-deoxyglucose-3(5)-epimerase and thymidine diphospho-4-ketodeoxyhexulose reductase, respectively. Inactivation of each of the three genes by gene disruption and replacement in the wild-type strain demonstrated that all of them are required for daunosamine biosynthesis.
Otten
S L
SL
School of Pharmacy, University of Wisconsin, Madison 53706, USA.
Gallo
M A
MA
Madduri
K
K
Liu
X
X
Hutchinson
C R
CR
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002237:Carbohydrate Dehydrogenases / Q000235:genetics*; D002238:Carbohydrate Epimerases / Q000235:genetics*; D003001:Cloning, Molecular; D003630:Daunorubicin / Q000096:biosynthesis*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D002237:Carbohydrate Dehydrogenases; C107149:dnmV protein, Streptomyces peucetius; D002238:Carbohydrate Epimerases; C085762:thymidine-diphospho-4-keto-6-deoxyglucose epimerase; D003630:Daunorubicin