Chloroquine induces basophilic differentiation of HL-60 cells.
Many agents have been known to induce the differentiation of HL-60 cells. However, only a small number of reports on the basophilic differentiation of this cell line are known. In this study we show that the exposure of HL-60 cells to chloroquine induces to differentiate into basophils. This chloroquine-induced change suggests that the increase in intracellular pH and the upregulation of p21 with subsequent downregulation of cdc2 kinase are triggers for basophilic differentiation of this cell line.
Sasaki
R
R
Division of Hematology, Jichi Medical School, Tochigi, Japan.
Furukawa
Y
Y
Okada
K
K
Nakamura
M
M
Yamane
Y
Y
Miura
Y
Y
Eguchi
M
M
D001491:Basophils / Q000166:cytology* / Q000187:drug effects; D016203:CDC2 Protein Kinase / Q000096:biosynthesis; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002454:Cell Differentiation; D002738:Chloroquine / Q000494:pharmacology*; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis
D002738:Chloroquine; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D016203:CDC2 Protein Kinase; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)