Albumin gene expression during mouse odontogenesis.
Albumin protein is present in developing teeth of several species. Oligomer primers and cRNA probes specific for albumin were designed to perform RT-PCR, and for in situ hybridization, respectively. In situ hybridization failed to reveal albumin expression in any tooth cells, however, albumin PCR products were amplified from tissues adhering to the roots of developing teeth from four-week-old mice. It is concluded that this source is not the primary source of albumin protein found in developing enamel, because of the location and level of expression of albumin mRNA in periodontal tissue.
Yuan
Z A
ZA
Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104, USA.
McAndrew
K S
KS
Collier
P M
PM
Koyama
E
E
Chen
E
E
Sandgren
E P
EP
Gibson
C W
CW
D000418:Albumins / Q000096:biosynthesis* / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D003743:Dental Enamel / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D008822:Mice, Transgenic / Q000235:genetics; D008963:Molar / Q000166:cytology / Q000378:metabolism; D009805:Odontogenesis / Q000502:physiology*; D009808:Odontogenic Tumors / Q000378:metabolism / Q000473:pathology; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis
D000418:Albumins; D012333:RNA, Messenger