title: Control of hypertrophic scar growth using selective photothermolysis.
abstract: OBJECTIVE
  Previous studies have shown a clinical improvement of hypertrophic scars (HS) after treatment with a pulsed dye laser. The objective of this study was to investigate the effects of variations in pulse wavelength and energy density on HS tissue using human HS implanted in athymic mice.
  
  
  METHODS
  Small pieces (approximately 1 mm3) of HS tissue were implanted into athymic mice and allowed to grow for 5 days. The implant site was then exposed to a single 450 microseconds pulse, and implant growth and histology were monitored for an additional 12 days. Laser wavelength and energy density ranges tested were 585-600 nm and 2-10 J/cm2, respectively.
  
  
  RESULTS
  Using a wavelength of 585 nm, laser treatment inhibited implant growth by 70% at 6 J/cm2 and 92% at 10 J/cm2, respectively. The inhibitory effect decreased as the laser wavelength was increased from 585 to 600 nm. A widespread destruction of the implant microvasculature with a minor effect on surrounding extracellular matrix at the highest light dose were observed.
  
  
  CONCLUSIONS
  Pulsed laser treatment inhibits HS implant growth in nude mice. This effect is likely mediated by selective photo-thermolysis of the implant microvasculature.
authors.affiliation: Surgical Services, Massachusetts General Hospital, Boston 02114, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Reiken
authors.forename: S F
authors.initials: SF
authors.lastname: Wolfort
authors.forename: F
authors.initials: F
authors.lastname: Berthiaume
authors.forename: C
authors.initials: C
authors.lastname: Compton
authors.forename: R G
authors.initials: RG
authors.lastname: Tompkins
authors.forename: M L
authors.initials: ML
authors.lastname: Yarmush
mesh_terms: D000818:Animals; D017439:Cicatrix, Hypertrophic / Q000473:pathology / Q000517:prevention & control / Q000601:surgery*; D006801:Humans; D017075:Laser Coagulation; D051379:Mice; D008819:Mice, Nude; D013997:Time Factors; D014183:Transplantation, Heterologous