Cloning, sequencing and expression in MEL cells of a cDNA encoding the mouse ribosomal protein S5.
We describe the isolation and characterization of a cDNA encoding the mouse S5 ribosomal protein. It was isolated from a MEL (murine erythroleukemia) cell cDNA library by differential hybridization as a down regulated sequence during HMBA-induced differentiation. Northern series analysis showed that S5 mRNA expression is reduced 5-fold throughout the differentiation process. The mouse S5 mRNA is 760 bp long and encodes for a 204 amino acid protein with 94% homology with the human and rat S5.
Vanegas
N
N
Departamento de Biologia Celular y del Desarrollo, Centro de Investigaciones Biológicas (CSIC), Velázquez, Madrid, Spain.
Castañeda
V
V
Santamaría
D
D
Hernández
P
P
Schvartzman
J B
JB
Krimer
D B
DB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D051379:Mice; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D012269:Ribosomal Proteins; C030831:ribosomal protein S5