Study of biochemical substrate and role of metalloproteinases in fascia transversalis from hernial processes.
The aim of this study was to examine the fascia transversalis (FT) from patients with direct and indirect hernia in an attempt to identify possible differences between each type of hernia. FT samples were obtained from 36 patients presenting inguinal hernia (23 indirect hernia and 13 direct hernia) who underwent surgery. We have analysed the ultrastructure of the fascia surrounding the hernial lesions, the proline and lysine hydroxylation in the tissue, the type I-type III collagen ratio and the presence of metalloproteinases. We have not detected ultrastructural differences in the collagen fibrils from FT in direct and indirect hernias. However, the interfibrillar matrix was more abundant in direct hernias, showing abundant electron-dense particles. No differences in proline hydroxylation were observed between each type of hernia. A small decrease in lysine hydroxylation was detected in patients with direct hernia. Enzyme-linked immunosorbent assays (ELISAs) showed no statistically significant differences in the type I-type III collagen absorbance ratios. Immunohistochemistry revealed no differences in the expression of matrix metalloproteinase-1. FT from patients presenting direct hernia showed a very strong staining vs. metalloproteinase-2 when compared with that observed in indirect hernia.
Bellón
J M
JM
Department of Morphological Sciences and Surgery, Faculty of Medicine, University of Alcalá Madrid, Spain.
Buján
J
J
Honduvilla
N G
NG
Jurado
F
F
Gimeno
M J
MJ
Turnay
J
J
Olmo
N
N
Lizarbe
M A
MA
D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure; D017364:Collagenases / Q000378:metabolism; D005205:Fascia / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D018093:Gelatinases / Q000378:metabolism; D006552:Hernia, Inguinal / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006900:Hydroxylation; D007150:Immunohistochemistry; D008239:Lysine / Q000378:metabolism; D020781:Matrix Metalloproteinase 1; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000378:metabolism*; D008854:Microscopy, Electron; D011392:Proline / Q000378:metabolism; D013379:Substrate Specificity
D003094:Collagen; D011392:Proline; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1; D008239:Lysine