Deoxycytidine kinase mRNA expression in childhood acute lymphoblastic leukemia.
Initial and relapsed childhood acute lymphoblastic leukemias (ALL) were investigated for mutations and expression of deoxycytidine kinase (dCK). dCK mediating toxicity of 1-beta-D-arabinofuranosylcytosine was analyzed semiquantitatively by RT-PCR and by single-strand conformation polymorphism. We found that patients with initial ALL experienced more frequently a relapse if dCK expression was low or absent. No mutations were found in initial and relapsed ALL.
Stammler
G
G
German Cancer Research Centre, Department 0510, Heidelberg.
Zintl
F
F
Sauerbrey
A
A
Volm
M
M
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D001215:Asparaginase / Q000627:therapeutic use; D002478:Cells, Cultured; D002648:Child; D003630:Daunorubicin / Q000627:therapeutic use; D003842:Deoxycytidine Kinase / Q000096:biosynthesis*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000201:enzymology*; D011241:Prednisone / Q000627:therapeutic use; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D014750:Vincristine / Q000627:therapeutic use
D012333:RNA, Messenger; D014750:Vincristine; D003842:Deoxycytidine Kinase; D001215:Asparaginase; D011241:Prednisone; D003630:Daunorubicin