Determination of endogenous peptides with in vitro ACE inhibitory activity in normotensive human plasma by the fluorometric HPLC method.
An in vitro degradation test of angiotensin (ANG) II or III in normotensive supine human plasma from 9 healthy male subjects confirmed the production of smaller ANG metabolites with angiotensin I-converting enzyme inhibitory activity. These metabolites were identified as ANG (3-8), ANG (5-8), and ANG (3-4), whose respective peptide concentrations were determined by our proposed naphthalene-2,3-dialdehyde (NDA)-HPLC method to be 64 +/- 9, 39 +/- 5, 176 +/- 22, and 197 +/- 35 fmol/ml of plasma.
Matsui
T
T
Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Matsufuji
H
H
Kawasaki
T
T
Osajima
Y
Y
D000328:Adult; D000804:Angiotensin II / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D000805:Angiotensin III / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents / Q000737:chemistry; D008297:Male; D009281:Naphthalenes / Q000737:chemistry; D010455:Peptides / Q000097:blood*; D011863:Radioimmunoassay; D013050:Spectrometry, Fluorescence; D016683:Supine Position
D000806:Angiotensin-Converting Enzyme Inhibitors; D007202:Indicators and Reagents; D009281:Naphthalenes; D010455:Peptides; D000804:Angiotensin II; D000805:Angiotensin III; C027717:2,3-naphthalenedicarboxaldehyde