Establishment of HTLV-1 carrier mice by injection with HTLV-1-producing T cells.
We injected with HTLV-1-producing T-cells, MT-2, into C3H/HeJ and Balb/c mice intraperitoneally within 24 hours after birth and at one week old. At 3 months old, HTLV-1 provirus was detected in peripheral blood mononuclear cells in both mice. It was also detected in lymph nodes, thymus, spleen and liver of those mice. The antibody response against HTLV-1 Gag antigen was detected in some of Balb/c mice. These findings suggest that the C3H/HeJ and Balb/c mice were infected with HTLV-1 persistently. The HTLV-1-infected mice should be helpful for studying the pathological state of HTLV-1 carriers and for an establishment of a small animal model of HTLV-1 related diseases including ATL.
Kushida
S
S
Department of Biochemistry, University of Tsukuba, Ibaraki, Japan.
Maeda
N
N
Fang
J
J
Uchida
K
K
Miwa
M
M
D000818:Animals; D002353:Carrier State / Q000473:pathology / Q000821:virology*; D004195:Disease Models, Animal; D005260:Female; D015683:Gene Products, gag / Q000032:analysis; D015490:HTLV-I Infections / Q000473:pathology / Q000635:transmission*; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification*; D018445:Infectious Disease Transmission, Vertical; D007963:Leukocytes, Mononuclear / Q000821:virology; D008099:Liver / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology; D011533:Proviruses / Q000302:isolation & purification; D013154:Spleen / Q000821:virology; D013950:Thymus Gland / Q000821:virology
D015683:Gene Products, gag