title: Efficacy of hand washing procedures on bacterial contamination of hydrogel contact lenses.
abstract: The effect of various hand washing regimens on transfer of bacterial contaminants from the hands to a hydrogel contact lenses was evaluated. Each of 47 subjects performed 5 different hand washing procedures, and then handled a new, sterile hydrogel contact lens. The lenses were cultured to determine colony-forming units (CFUs) and microbial identity. Median CFUs on lenses handled after washing with water, soap and water, or soap and water followed by towel drying were higher than the median CFU for lenses handled after no hand washing. The median CFU for lenses handled after soap and water washing followed by an alcohol wipe was not different from the no washing group. The majority of the contaminants were identified as Staphylococcus epidermidis. These results show that ordinary hand washing alone does not decrease, and may even increase, the amount of contaminants transferred from the hands to a hydrogel lens. Use of an alcoholic wipe after hand washing reverses this effect. Hand washing is still recommended in contact lens hygiene for removal of more pathogenic contaminants.
authors.affiliation: Southern California College of Optometry, Fullerton, USA.
authors.forename: V T
authors.initials: VT
authors.lastname: Ly
authors.forename: P A
authors.initials: PA
authors.lastname: Simmons
authors.forename: T B
authors.initials: TB
authors.lastname: Edrington
authors.forename: S
authors.initials: S
authors.lastname: Wechsler
authors.forename: P N
authors.initials: PN
authors.lastname: De Land
mesh_terms: D015169:Colony Count, Microbial; D003263:Contact Lenses, Hydrophilic; D004203:Disinfection / Q000379:methods; D004866:Equipment Contamination / Q000517:prevention & control; D005260:Female; D006235:Hand Disinfection / Q000379:methods*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006920:Hygiene / Q000592:standards; D008297:Male; D011092:Polyethylene Glycols; D013212:Staphylococcus epidermidis / Q000302:isolation & purification; D011795:Surveys and Questionnaires
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols