title: Distribution pattern of tenascin-C in normal and neoplastic mesenchymal tissues.
abstract: Descriptions for tenascin-C distribution are largely restricted to epithelial tumours. The present study utilized newly developed and characterized monoclonal (hT191) and polyclonal antibodies to investigate the distribution pattern of tenascin-C in a panel of mesenchymal tumours, which was contrasted with normal tissue. The specific antibodies recognized the distinctive star-like hexabrachion protein isolated from transformed cell-culture medium and serum from normal individuals. In normal tissues, a strong tenascin-C expression in the extracellular matrix was largely restricted to basement-membrane regions of epithelium and tonsilar sinusoids, pericellularly within smooth-muscle bundles, associated with perimysial, -chondrial, -neurial and -tendon surfaces, and diffusely within vascular adventitia. It was found in the corresponding tumours of the neural sheath (schwannoma) and smooth muscle (leiomyosarcoma), and was abundantly present around certain blood vessels of mesenchymal tumours. Although not detected in normal muscle, or in adipose or fibrous connective tissue, neo-expression of tenascin-C was shown in more than half of the rhabdomyosarcomas, fibromas and liposarcomas, with an increased positive percentage in variably malignant myxoid liposarcomas compared with lipoma-like sarcomas. Tenascin-C was typically found in the extracellular matrix of soft-tissue tumours, but was notably absent from the epithelial-cell components of mixed epithelial/mesenchymal tumours. Its apparently enhanced expression in soft-tissue tumours differs from that of most other large extracellular-matrix proteins, suggesting possible functional involvement of the cell-adhesion molecule, tenascin-C, in the neoplastic phenotype.
authors.affiliation: Laboratory of Biochemistry, Federal Institute of Technology-ETH, Zurich, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Schnyder
authors.forename: R O
authors.initials: RO
authors.lastname: Semadeni
authors.forename: R W
authors.initials: RW
authors.lastname: Fischer
authors.forename: L
authors.initials: L
authors.lastname: Vaughan
authors.forename: B D
authors.initials: BD
authors.lastname: Car
authors.forename: P U
authors.initials: PU
authors.lastname: Heitz
authors.forename: K H
authors.initials: KH
authors.lastname: Winterhalter
authors.forename: B F
authors.initials: BF
authors.lastname: Odermatt
mesh_terms: D000906:Antibodies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008637:Mesenchymoma / Q000378:metabolism*; D008648:Mesoderm / Q000378:metabolism*; D011247:Pregnancy; D019063:Tenascin / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D019063:Tenascin