title: The influence of physical restraint or fasting on plaque-forming cell response in mice.
abstract: This study was designed to investigate the effects of 1- and 3-day (16 h/day) physically restrained or fasting on immunological and endocrine responses in CBF1 mice. The influence of stressors on these responses was evaluated using anti-sheep red blood cell plaque-forming assay, and by examining T cell subsets, thymus weight and endocrine hormone levels. The results revealed that a significant elevation of the plaque-forming cells (PFC) was found in spleen cells in 1-day restrained mice, that the PFC were conversely suppressed following 3-day physically restrained stress, and that the PFC were not affected by 1- or 3-day fasting stress. Serum levels of norepinephrine were found to be significantly increased only in 1-day physically restrained mice. No change of T cell subsets and thymus weight was found in 1-day physically restrained mice. A significant increase in serum corticosterone levels was elicited in both 1- and 3-day physically restrained mice, and 3-day fasting mice, while increased Lyt2-positive T cell and thymic atrophy were found only in 3-day physically restrained mice. These findings suggest that immune function was differentially affected by the duration and types of stressors.
authors.affiliation: Department of Psychiatry, Mie University School of Medicine, Tsu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Komori
authors.forename: R
authors.initials: R
authors.lastname: Fujiwara
authors.forename: K
authors.initials: K
authors.lastname: Shizuya
authors.forename: S
authors.initials: S
authors.lastname: Miyahara
authors.forename: J
authors.initials: J
authors.lastname: Nomura
mesh_terms: D000818:Animals; D000917:Antibody Formation; D003345:Corticosterone / Q000097:blood; D004837:Epinephrine / Q000097:blood; D005215:Fasting; D008297:Male; D051379:Mice; D009638:Norepinephrine / Q000097:blood; D012149:Restraint, Physical; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D003345:Corticosterone; D009638:Norepinephrine; D004837:Epinephrine