title: Type-4 pili of Kingella denitrificans.
abstract: Kingella denitrificans possess type-4 pili, and the type strain, ATCC 33394, contains at least four complete copies of type-4 pilin-encoding genes. Previously reported hybridization patterns of K. denitrificans chromosomal DNA seen using a Neisseria gonorrhoeae pilin gene region probe, had been interpreted as representing possible partial, silent gene loci. This now appears to be due to cross-reaction to multiple copies of 18-bp inverted repeat structures. Data are presented on a variety of colony variants which have changed from a spreading-corroding (SC) phenotype to a nonspreading-noncorroding (N) phenotype. Interestingly, while the SC to N transition is most often associated with loss of piliation in other bacteria containing type-4 pili, many of the K. denitrificans N variants still produce pilin, and some still produce pili.
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts School of Medicine, Boston, MA 02111, USA.
authors.forename: S
authors.initials: S
authors.lastname: Weir
authors.forename: L W
authors.initials: LW
authors.lastname: Lee
authors.forename: C F
authors.initials: CF
authors.lastname: Marrs
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015139:Blotting, Southern; D015153:Blotting, Western; D015342:DNA Probes; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry* / Q000235:genetics; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D017881:Kingella / Q000737:chemistry* / Q000235:genetics / Q000472:pathogenicity; D008854:Microscopy, Electron; D010641:Phenotype
chemical_list: D001425:Bacterial Outer Membrane Proteins; D015342:DNA Probes; D036781:Fimbriae Proteins