Cloning of equine type II procollagen and the modulation of its expression in cultured equine articular chondrocytes.
The complete nucleotide sequence of equine type II procollagen has not been previously reported, and equine-specific probes have not been available. We report the complete sequence and discuss the molecular characteristics of equine type II procollagen mRNA which was cloned from a cDNA library prepared from mRNA isolated from equine articular chondrocytes. The coding sequence (4257 bp) was 92.4% homologous to the cDNA of the human sequence, and the propeptide was 97% identical to the human sequence. We demonstrated that when equine chondrocytes are grown in phenotypically-maintained cultures, the expression of type II procollagen is linked to the age of the animal. Additionally, in cultures of young equine chondrocytes, IL1-beta and TNF-alpha both reduced the expression of type II procollagen mRNA in a dose responsive manner.
Richardson
D W
DW
Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, USA.
Dodge
G R
GR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002358:Cartilage, Articular / Q000166:cytology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006736:Horses / Q000235:genetics*; D008969:Molecular Sequence Data; D011347:Procollagen / Q000235:genetics*
D011347:Procollagen