An evaluation of nine commercial EIA kits for the detection of measles specific IgG.
Nine commercial EIAs for measles-specific IgG were compared with haemagglutination inhibition (HI) and plaque reduction neutralization (PRN). A total of 174 sera selected, to give approximately half of the sera without measles antibody by HI, were tested by all EIAs and HI. However, there was sufficient volume of only 101 samples for testing by PRN. A dilution curve of the British Standard measles antibody serum was also tested by each EIA. Assays were evaluated qualitatively against a consensus EIA result, HI and PRN: Gull, Melotest and Behring EIAs performed best. Quantitative evaluation was by assessment of the characteristics of the standard dilution curve, and by plotting differences with PRN against mean: Gull and Melotest EIAs were best.
Hesketh
L
L
Public Health Laboratory Service Seroepidemiology Unit, Royal Preston Hospital, UK.
Charlett
A
A
Farrington
P
P
Miller
E
E
Forsey
T
T
Morgan-Capner
P
P
D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood*; D008457:Measles / Q000276:immunology*; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic