Restriction endonuclease analysis, DNA relatedness and phenotypic characterization of Campylobacter jejuni and Camp. coli isolates involved in food-borne disease.
Seven cases of Campylobacter infection, each of them involving two isolates, were analysed. Study of their biochemical profiles and susceptibility patterns allowed the identification of Camp. jejuni and Camp. coli isolates and the effective typing of Camp. jejuni strains into biotypes. Genotyping was carried out by comparing chromosomal DNA restriction patterns obtained by cleavage with Bg/II and EcoRV and by Southern hybridization experiments. These studies revealed clonal homogeneity between both isolates in five of the seven cases studied, indicating that in these cases Campylobacter infection was caused by a single strain. Infection with two different strains was characterized in only two of the seven cases studied, two different species belonging to Camp. coli and Camp. jejuni ssp. jejuni biotype 1 being identified. Genetic analysis proved to be the most reliable technique to achieve precise identification of strains and to elucidate clonal heterogeneity among Campylobacter isolates obtained from a single patient.
Jiménez
A
A
Department of Analytical Chemistry, Nutrition and Food Science, University of Santiago de Compostela, Lugo, Spain.
Barros-Velázquez
J
J
Rodríguez
J
J
Villa
T G
TG
D017000:Campylobacter coli / Q000235:genetics / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D005506:Food Contamination; D005516:Food Microbiology; D006801:Humans; D015183:Restriction Mapping
D004269:DNA, Bacterial