Detection of tricyclic antidepressants in whole blood by headspace solid-phase microextraction and capillary gas chromatography.
A simple method for the extraction of four tricyclic antidepressants from whole blood by headspace solid-phase microextraction (SPME) is presented. The whole blood samples contain four drugs (amitriptyline, chlorimipramine, imipramine, and trimipramine) and are heated at 100 degrees C in a septum-capped vial in the presence of distilled water and NaOH solution; a polydimethylsiloxane-coated SPME fiber is exposed to the headspace of the vial to allow adsorption of the drugs before capillary gas chromatography (GC) with flame-ionization detection. The headspace SPME-GC produces intense peaks for each drug with very little background noise. Recoveries of the four drugs by the present method are 5.3-12.9%. The calibration curves for the drugs show linearity in the range of 31-1000 ng/0.5 mL. The detection limits of each drug are 16-25 ng/0.5 mL. Imipramine is detectable from rat blood 5 h after oral administration of imipramine (500 mg/kg body weight); the concentration is 1.44 +/- 0.209 micrograms/mL.
Lee
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XP
Department of Legal Medicine, Showa University School of Medicine, Tokyo, Japan.
Kumazawa
T
T
Sato
K
K
Suzuki
O
O
D000639:Amitriptyline / Q000097:blood*; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D002997:Clomipramine / Q000097:blood*; D006801:Humans; D007099:Imipramine / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012972:Sodium Hydroxide / Q000737:chemistry; D014299:Trimipramine / Q000097:blood*; D014867:Water / Q000737:chemistry
D000929:Antidepressive Agents, Tricyclic; D014867:Water; D000639:Amitriptyline; D012972:Sodium Hydroxide; D014299:Trimipramine; D002997:Clomipramine; D007099:Imipramine