Singlet oxygen producing photosensitizers in photophoresis.
In this study, the immunosuppressive properties of two photosensitizers (benzoporphyrin derivative monoacid ring A (BPD) and Photofrin (HPD)), used for the photodynamic therapy of cancer, were investigated. The investigations were performed in our rat model for photophoresis. The validity of this model has been amply demonstrated. It enables a distinction to be made between antigen-specific and antigen non-specific immune suppression. With this model, the immune response which can be specifically suppressed is the contact hypersensitivity (CHS). CHS is induced by 2,4-dinitrofluorobenzene (DNFB). Both BPD and HPD are able to suppress CHS induced by DNFB. Furthermore, this generated suppression is transferable by the spleen cells of treated animals and is antigen non-specific.
van Iperen
H P
HP
Leiden/Amsterdam Centre for Drug Research, Department of Medicinal Photochemistry, Netherlands. iperenh@chem.leidenuniv.nl
Beijersbergen van Henegouwen
G M
GM
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D017324:Hematoporphyrin Derivative / Q000276:immunology / Q000378:metabolism*; D007166:Immunosuppressive Agents / Q000276:immunology / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D017893:Photopheresis; D017319:Photosensitizing Agents / Q000276:immunology / Q000378:metabolism*; D010853:Picryl Chloride / Q000494:pharmacology; D011166:Porphyrins / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D026082:Singlet Oxygen
D007166:Immunosuppressive Agents; D017319:Photosensitizing Agents; D011166:Porphyrins; C504224:benzoporphyrin D; D026082:Singlet Oxygen; D017324:Hematoporphyrin Derivative; D004139:Dinitrofluorobenzene; D010100:Oxygen; D010853:Picryl Chloride