title: [Development of monoclonal antibody-based immunoenzyme kit for detection of human thyrotropin hormone].
abstract: An enzyme immunoassay kit based on monoclonal antibodies (Mab) has been developed for measuring thyroid-stimulating hormone in human serum and plasma. Laboratory trials demonstrated its high sensitivity (3.1 microIU/ml), specificity, reliability, and accuracy. The analysis is simple and rapid (2.5 h), which is convenient for clinical use. The kit permits differentiation between normal subjects and patients with primary hypo- or hyperthyroidism and helps monitor the treatment efficacy. Comparison with the Amerlite TSH-60 kit, carried out on 357 sera, showed a high correlation coefficient (0.96) between the two kits.
authors.forename: E R
authors.initials: ER
authors.lastname: Sigal
authors.forename: L K
authors.initials: LK
authors.lastname: Koriazova
authors.forename: G I
authors.initials: GI
authors.lastname: Kovalevskaia
authors.forename: T A
authors.initials: TA
authors.lastname: Pozdniakova
authors.forename: E I
authors.initials: EI
authors.lastname: Kandyba
authors.forename: V A
authors.initials: VA
authors.lastname: Kuznetsov
authors.forename: R G
authors.initials: RG
authors.lastname: Vasilov
mesh_terms: D000911:Antibodies, Monoclonal; D003937:Diagnosis, Differential; D006801:Humans; D006980:Hyperthyroidism / Q000175:diagnosis; D007037:Hypothyroidism / Q000175:diagnosis; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013972:Thyrotropin / Q000097:blood*
chemical_list: D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic; D013972:Thyrotropin