Immunomagnetic separation and conventional culture procedure for detection of naturally occurring Salmonella in raw pork sausages and chicken meat.
The aim of the study was to compare immunomagnetic separation (IMS) and conventional selective enrichment procedures using selenite cystine broth (SC) and Rappaport-Vassiliadis broth (RV) in 137 naturally contaminated food samples (69 raw pork sausages and 68 chicken meat). The utilization of SC or IMS appeared to be the most appropriate enrichment procedure: 15 out of 18 Salmonella-positive samples (83.3%) were detected by SC and 12 (66.7%) by IMS; RV yielded only seven positive isolations (38.9%). However, RV yielded the highest count of Salmonella colonies per plate and the lowest interference by competing organisms. IMS could become a reliable alternative to standard enrichment procedures and a combined IMS and selective enrichment broth could increase the chance of Salmonella recovery.
Ripabelli
G
G
Department of Animal, Plant and Environmental Sciences, University of Molise, Campobasso, Italy.
Sammarco
M L
ML
Ruberto
A
A
Iannitto
G
G
Grasso
G M
GM
D000818:Animals; D001431:Bacteriological Techniques; D002645:Chickens; D015169:Colony Count, Microbial; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008460:Meat / Q000009:adverse effects / Q000382:microbiology*; D008461:Meat Products / Q000009:adverse effects / Q000382:microbiology*; D012475:Salmonella / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D012478:Salmonella Food Poisoning / Q000209:etiology; D013552:Swine
D003470:Culture Media