Conversion of in vitro cultured human monocytes into effective presenters of an HER2/neu-encoded CTL peptide epitope.
Tumour-derived peptides have been surveyed, in a variety of systems, for their ability to elicit cytokine release from class I restricted T cells. Analogous studies on ovarian carcinoma have employed the antigen-processing defective T2 cell line, Purified dendritic cells (DC) have been reported to act as highly effective APC. A facile method was developed whereby DC-like cells were generated from monocyte precursors. Herein, evidence is presented suggesting DC-like cells are superior to T2 with respect to their ability to present a defined CTL epitope associated with ovarian carcinoma.
Wasserman
K
K
Laboratory of Experimental Immunology, NCI-FCRDC, Frederick, MD, USA.
Corsi
M M
MM
Szekel
L
L
Kono
K
K
Maes
H H
HH
Kiessling
R
R
D000938:Antigen-Presenting Cells / Q000378:metabolism*; D002277:Carcinoma; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media; D016207:Cytokines / Q000494:pharmacology; D003713:Dendritic Cells / Q000378:metabolism; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D005780:Gelatin; D018734:Genes, erbB-2 / Q000276:immunology*; D006801:Humans; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D010051:Ovarian Neoplasms; D010455:Peptides / Q000276:immunology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D005780:Gelatin; D010455:Peptides; D018719:Erb-b2 Receptor Tyrosine Kinases