title: Androgen deprivation causes up-regulation of androgen receptor transcript in the rat prostate.
abstract: We have studied the effect of androgenic deprivation on the level of androgen receptor transcript in the rat ventral prostate. The rats were treated with estradiol benzoate, flutamide and [D Trp6, des Gly10]gonadotropin releasing hormone (GnRH) for different time periods. These treatments produced a significant decrease in the weight of prostate. Total RNA isolated from the ventral prostates was hybridized with the cDNA probe for androgen receptor. Densitometric analysis of the autoradiographic signal revealed a rise in the level of androgen receptor RNA following treatment of rats with estradiol benzoate and flutamide. Treatment of rats with [D Trp6, des Gly10] GnRH brought about a transient rise in the level of androgen receptor RNA. Thus, our results indicate that androgenic deprivation up-regulates the level of androgen receptor transcript.
authors.affiliation: Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: V L
authors.initials: VL
authors.lastname: Kumar
authors.forename: P K
authors.initials: PK
authors.lastname: Majumder
authors.forename: V
authors.initials: V
authors.lastname: Kumar
mesh_terms: D000728:Androgens / Q000378:metabolism*; D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D005485:Flutamide / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D008297:Male; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000728:Androgens; D012333:RNA, Messenger; D011944:Receptors, Androgen; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D005485:Flutamide