A human B-lymphoblastoid cell line constitutively producing Epstein-Barr herpesvirus and JHK retrovirus.
The human B-lymphoblastoid cell line, designated JHK-3, with pre-B-cell characteristics, chronically produces two viruses, Epstein-Barr virus (EBV) and JHK virus, an apparently novel retrovirus. The JHK-3 cells are much more productive of extracellular EBV than the high-producer marmoset line B95-8. The extracellular virus of the JHK-3 EBV strain is relatively fragile, more broadly dispersed in an ultracentrifuged sucrose gradient than the B95-8 EBV and more susceptible to disruption by combined treatment with urea and dithiothreitol. By restriction fragment length polymorphism analysis, the JHK-3 EBV strain resembles the EBV strain FF-41. The JHK-3 cells also produce an incompletely characterized, relatively fragile, enveloped, icosahedral RNA virus that contains Mn(++)-dependent reverse transcriptase. JHK virions measure 85 nm in ultrathin sections, much smaller than other Retroviridae. The JHK virus exhibits a distinctive morphogenesis, most nearly resembling C-type retroviruses. The JHK-3 cell line provides a human cell model for investigating virus/virus interactions and their pathogenetic affects on host cells which chronically and simultaneously produce DNA and RNA viruses.
Grossberg
S E
SE
Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226, USA.
Kushnaryov
V M
VM
Cashdollar
L W
LW
Raisch
K P
KP
Miller
G
G
Sun
H Y
HY
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000821:virology*; D002144:Callithrix; D002460:Cell Line; D004279:DNA, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D012191:Retroviridae Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D004279:DNA, Viral; D012191:Retroviridae Proteins; D012194:RNA-Directed DNA Polymerase