Enzymatic hydrolysis of the conjugate of vitamin D and related compounds.
The monoglucuronides of vitamin D, 25-hydroxyvitamin D and the corresponding pro-forms were subjected to enzymatic hydrolysis using beta-glucuronidase, and substrate specificities were found in the examined enzymes originating from different sources, which were determined using reversed-phase high-performance liquid chromatography with UV detection. The enzymatic hydrolysis of the corresponding monosulfates was also examined using the same system.
Shimada
K
K
Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Mitamura
K
K
Saito
K
K
Ohtake
Y
Y
Nakatani
I
I
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005966:Glucuronidase / Q000737:chemistry*; D006868:Hydrolysis; D013379:Substrate Specificity; D013429:Sulfatases / Q000737:chemistry*; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000737:chemistry*
D014807:Vitamin D; C104450:25-hydroxyvitamin D; D013429:Sulfatases; D005966:Glucuronidase