Equine inhibin/activin beta A-subunit mRNA is expressed in the endometrial gland, but not in the trophoblast, during pregnancy.
The expression of both inhibin alpha- and inhibin/activin beta A-subunit mRNA was examined in equine uteroplacental tissues collected during pregnancy (days 90 to 300). Northern blot analysis revealed that 5 transcripts (7.0, 4.1, 3.4, 2.6, 1.5 kb) of beta A-subunit were present, and the most abundantly expressed transcript was the 1.5 kb one. Relatively high levels of the 1.5 kb transcript were seen in the second trimester of pregnancy compared to what was found in the third trimester. To identify the tissue localization of beta A-subunit mRNA, in situ hybridization was performed, and the positive signal was observed exclusively in the endometrial glands, but not in the fetal placental tissue (trophoblast) at days 150, 210, and 300 of pregnancy. On the other hand, inhibin alpha-subunit transcript could not be detected at any stage of pregnancy examined either by Northern blot analysis or in situ hybridization. Although the factor(s) regulating the gene expression of beta A-subunit in this equine tissue is currently unknown, these results suggest that activin, but not inhibin, is predominantly produced in the endometrial glands of the pregnant mare, and thus produced activin may play a paracrine or endocrine role during pregnancy in this species.
Yamanouchi
K
K
Department of Veterinary Physiology, Veterinary Medical Science, University of Tokyo, Japan.
Hirasawa
K
K
Hasegawa
T
T
Ikeda
A
A
Chang
K T
KT
Matsuyama
S
S
Nishihara
M
M
Miyazawa
K
K
Sawasaki
T
T
Tojo
H
H
Tachi
C
C
Takahashi
M
M
D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D006736:Horses / Q000502:physiology*; D017403:In Situ Hybridization; D028322:Inhibin-beta Subunits; D007265:Inhibins / Q000096:biosynthesis / Q000235:genetics*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000235:genetics*; D016372:RNA, Antisense / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014327:Trophoblasts / Q000378:metabolism*
D015342:DNA Probes; D016372:RNA, Antisense; D012333:RNA, Messenger; C103008:inhibin beta A subunit; D007265:Inhibins; D028322:Inhibin-beta Subunits