title: The complete Mokola virus genome sequence: structure of the RNA-dependent RNA polymerase.
abstract: The genome sequence of the rabies-related virus Mokola virus (genus Lyssavirus) has been completed by sequencing the L gene, which consists of 6384 nucleotides encoding a 2127 amino acid polymerase. Alignment of the Mokola virus L protein with other polymerases from the virus order Mononegavirales defined three domains: a divergent NH2-terminal domain, a highly conserved central domain carrying most of the functional motifs and a COOH-terminal domain with alternating conserved and divergent regions. A statistical study outlined the stringency of conservation of glycine, acidic (D, E) and basic (K, R, H) amino acids in polymerases, particularly as key residues of the conserved motifs.
authors.affiliation: Laboratoire des Lyssavirus, Institut Pasteur, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Le Mercier
authors.forename: Y
authors.initials: Y
authors.lastname: Jacob
authors.forename: N
authors.initials: N
authors.lastname: Tordo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D004279:DNA, Viral; D016679:Genome, Viral; D006801:Humans; D018114:Lyssavirus / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase