title: Both A type and B type Epstein-Barr virus nuclear antigen 6 interact with RBP-2N.
abstract: Using the yeast two-hybrid system, Epstein-Barr virus nuclear antigen 6A (EBNA6A) was found to interact with the RBP-2N isoform of RBP-J kappa. The interaction of EBNA6A and EBNA6B with RBP-2N was compared and the results indicated that EBNA6B was less efficient at interacting with RBP-2N than was EBNA6A. Deletion mutation analysis of EBNA6A identified a region involved in the interaction with RBP-2N, while analysis of RBP-2N identified a domain which interacts with EBNA6A. The region of RBP-2N to which EBNA6A binds has previously been shown to interact with EBNA2.
authors.affiliation: Queensland Institute of Medical Research, EBV Unit, Bancroft Centre, Herston Queensland, Australia. davidY@qimr.edu.au
authors.forename: D B
authors.initials: DB
authors.lastname: Young
authors.forename: K
authors.initials: K
authors.lastname: Krauer
authors.forename: N
authors.initials: N
authors.lastname: Kienzle
authors.forename: T
authors.initials: T
authors.lastname: Sculley
mesh_terms: D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D016296:Mutagenesis; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; C086925:RBP-2N protein, human; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins