Densitometric evaluation of markers for cholinergic transmission in rat superior olivary complex.
Cholinergic transmission in the rat superior olivary complex (SOC) was assessed by densitometric measurements of labeling with several markers: acetylcholinesterase (AChE) histochemistry, choline acetyltransferase (ChAT) immunohistochemistry, and muscarinic acetylcholine receptor (M35) immunohistochemistry and N-methylscopolamine (NMS) binding autoradiography, as well as its subtype 2 (m2) immunohistochemistry. The markers which may occur in cholinergic neurons (ChAT, m2 receptor and AChE) showed dense labeling in the ventral nucleus of the trapezoid body (VNTB), in line with other evidence that it contains cholinergic neuron somata. The markers which are predominantly in cholinoceptive neurons (M35 and NMS) showed less labeling in the SOC, suggesting few muscarinic cholinergic inputs. These results are compared with those for the cochlear nucleus.
Yao
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Department of Otolaryngology, Head and Neck Surgery, Medical College of Ohio, Toledo 43699-0008, USA. wyao@gemini.mco.edu
Godfrey
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DA
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D015415:Biomarkers; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003720:Densitometry; D007150:Immunohistochemistry; D009847:Olivary Nucleus / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D010275:Parasympathetic Nervous System / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology*
D015415:Biomarkers; D011950:Receptors, Cholinergic; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase