Factors regulating production of alpha-galactosidase from Bacillus sp. JF2.
Certain factors affecting the production of cell-associated alpha-galactosidase by Bacillus sp. JF2 were investigated. The intention was to maximize alpha-galactosidase activity of potential commercial application, by consecutive optimization of growth media and conditions. The highest alpha-galactosidase activity was obtained when grown on melibiose, whereas sucrose inhibited the production of alpha-galactosidase, alpha-Galactosidase production was optimally active at pH 7.5 and 55 degrees C. It was identified that a soy effluent stream could be used as the best carbon source for alpha-galactosidase by Bacillus sp. JF2.
Li
X
X
Department of Food Technology, Dalian College of Light Industry, China.
Yang
L
L
Yan
P
P
Zuo
F
F
Jin
F
F
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D000519:alpha-Galactosidase / Q000096:biosynthesis*
D000519:alpha-Galactosidase