Cloning and overexpression of polypeptide release factor 1 of Thermus thermophilus.
A prfA gene encoding polypeptide release factor RF1 was cloned from Thermus thermophilus. T thermophilus RF1 shares 68% homology with Escherichia coli RF1, and its overproduction reduced readthrough translation of UAG, not of UGA, in the lacZ gene. Rapid purification of T thermophilus RF1 was achieved by T7-RNA polymerase driven overexpression of T thermophilus RF1 protein with a C-terminal histidine tag.
Ito
K
K
Department of Tumor Biology, Institute of Medical Science, University of Tokyo, Japan.
Nakamura
Y
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010454:Peptide Termination Factors; D016963:Thermus thermophilus / Q000235:genetics; D015534:Trans-Activators / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*
D001426:Bacterial Proteins; D010454:Peptide Termination Factors; D015534:Trans-Activators