Antiserum to growth hormone decreases sleep in the rat.
To determine whether an acute withdrawal of growth hormone (GH) alters sleep, the effects of antiserum to GH (GH-AS) on sleep were studied in the rat. Sleep-wake activity and cortical brain temperature (Tc) were recorded for 2 days after systemic injection of physiological saline. Then, one group of rats (n = 6) received GH-AS whereas another group of rats was injected with normal rabbit serum (n = 6). The injections were given 1 h before light onset, and the rats' behaviors were recorded for 23 h during the subsequent 12-hour light and 12-hour dark period. Sleep and Tc were not altered after normal rabbit serum. The durations of both rapid eye movement sleep (REMS) and non-REMS (NREMS), and the EEG slow-wave activity during NREMS were significantly suppressed during the light period following the injection of GH-AS. Tc tended to decrease for 3 h and a small rise was observed thereafter during the light period, but these changes were not statistically significant. The assay of GH in plasma samples obtained at 30-min intervals for 5 h after injection of normal rabbit serum or GH-AS verified the decreases in plasma GH concentrations in response to GH-AS. It is suggested that GH may promote sleep possibly via some metabolic actions.
Obál
F
F
Department of Physiology, A. Szent-Györgyi Medical University, Szeged, Hungary.
Bodosi
B
B
Szilágyi
A
A
Kacsóh
B
B
Krueger
J M
JM
D000818:Animals; D001831:Body Temperature; D001921:Brain / Q000502:physiology; D004569:Electroencephalography; D013006:Growth Hormone / Q000037:antagonists & inhibitors* / Q000097:blood / Q000502:physiology*; D007106:Immune Sera / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology
D007106:Immune Sera; D013006:Growth Hormone