The structural and functional basis of antibody catalysis.
Ten years have passed since the initial reports that antibodies could be programmed to have enzymatic activity by immunization with a transition-site analog. Much of the research over the last decade has focused on defining the scope and generality of antibody catalysis; however, during the past two years the first few crystal structures of catalytic antibody transition-state analogs have been reported. This review analyzes four such structures of catalytic antibodies that catalyze markedly different reactions, including ester hydrolysis, sulfide oxidation, and a pericyclic rearrangement. Structure-function relations for these catalysts are discussed and compared to the structure and function of natural enzymes, as well as the chemistry that occurs in solution.
Wade
H
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Department of Pharmaceutical Chemistry, University of California, San Francisco 94143, USA.
Scanlan
T S
TS
D017151:Antibodies, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D008958:Models, Molecular; D013329:Structure-Activity Relationship
D017151:Antibodies, Catalytic