The endotoxin of Helicobacter pylori is a modulator of host-dependent gastritis.
Atrophic gastritis caused by Helicobacter pylori is the precursor lesion in the development of intestinal-type gastric adenocarcinoma. In animal models, atrophic gastritis induced by Helicobacter felis has been shown to be host dependent, developing in some mouse strains and not in others. The lipopolysaccharide (LPS) of H. pylori has been suggested to play a role in the induction of gastritis. The goal of this study was to compare the inflammation induced by long-term infection of the C3H/He and the C3H/HeJ strains of mice with H. felis. C3H/HeJ mice are unresponsive to LPS. Six months after infection, severe atrophic gastritis had developed in the body mucosae of all infected C3H/He mice, with replacement of parietal and chief cells. Atrophy was associated with a loss of the H. felis from the antral mucosa. In contrast, no atrophy was seen in the infected C3H/HeJ non-LPS responder animals, and heavy colonization of the antrum remained. There were no significant differences between both the quantitative and qualitative serum immunoglobulin G (IgG) and salivary IgA levels in both strains of mice. The main difference between the two strains of long-term-infected mice was a lack of macrophage infiltration in the lamina propria. Immunization induced good protective immunity to challenge with viable H. felis. Helicobacter-induced, host-dependent gastritis is likely to be cell mediated. The C3H/He and C3H/HeJ mouse model provides an excellent opportunity to investigate the cellular basis of atrophic gastritis.
Sakagami
T
T
School of Microbiology and Immunology, The University of New South Wales, Sydney, Australia.
Vella
J
J
Dixon
M F
MF
O'Rourke
J
J
Radcliff
F
F
Sutton
P
P
Shimoyama
T
T
Beagley
K
K
Lee
A
A
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D005260:Female; D005757:Gastritis, Atrophic / Q000209:etiology* / Q000473:pathology; D016481:Helicobacter Infections / Q000276:immunology; D016480:Helicobacter pylori / Q000472:pathogenicity*; D007114:Immunization; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D008809:Mice, Inbred C3H
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides