Cloning and characterization of the rat p130, a member of the retinoblastoma gene family.
A cDNA clone encoding rat p130, a member of the retinoblastoma (Rb) gene family, was isolated based on the sequence homology of the E1A-binding domain. The 4.87 kb cDNA contained an 1135-amino acid open reading frame with high homologies to the human and mouse p130 and a partial homology to the pRb protein. p130 showed difference in distribution of potential phosphorylation sites from pRb in the N-terminal and the B pocket regions. p130 mRNA was detected in most rat tissues. The p130 gene was mapped to rat chromosome 19p11-13 by fluorescence in situ hybridization.
Sawada
Y
Y
Department of Molecular Biology, Cancer Research Institute, Sapporo Medical University School of Medicine, Japan. ysawada@sapmed.ac.jp
Nomura
H
H
Endo
Y
Y
Umeki
K
K
Fujita
T
T
Ohtaki
S
S
Fujinaga
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D011506:Proteins; D051381:Rats; D012175:Retinoblastoma / Q000235:genetics*; D050717:Retinoblastoma-Like Protein p130; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D010750:Phosphoproteins; D011506:Proteins; C493839:Rbl2 protein, rat; D050717:Retinoblastoma-Like Protein p130