Atherosclerosis and acute arterial thrombosis in rabbits: a model using balloon desendothelization without dietary intervention.
Acute thrombosis can be induced in rabbits by a triggering protocol using Russell's viper venom and histamine given after 8 months of a 1% cholesterol diet and balloon desendothelization. In the present study, we tested the hypothesis that aortic desendothelization performed 4 months before the triggering protocol without a high cholesterol diet is a highly effective and less expensive way of producing arterial atherosclerosis and thrombosis. Nineteen male New Zealand white rabbits on a normal diet were studied. The control group (N = 9) received no intervention during the 4-month observation period, while the other group (N = 10) was submitted to aortic balloon desendothelization using a 4F Fogarty catheter. At the end of this period, all animals were killed 48 h after receiving the first dose of the triggering treatment. Eight of 10 rabbits (80%) in the balloon-trauma group presented platelet-rich arterial thrombosis while none of the animals in the control group had thrombus formation (P < 0.01). Thus, this model, using balloon desendothelization without dietary manipulation, induces arterial atherosclerosis and thrombosis and may provide possibilities to test new therapeutic approaches.
Picon
P D
PD
Departamento de Farmacologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Brasil.
Gonçalves
S C
SC
Wainstein
M V
MV
Costa
A F
AF
Mengarda
C V
CV
Machado
R P
RP
Berlim
G L
GL
Edelweiss
M
M
Edelweiss
M I
MI
Ribeiro
J P
JP
D015906:Angioplasty, Balloon, Coronary; D000818:Animals; D001161:Arteriosclerosis / Q000628:therapy*; D004730:Endothelium, Vascular / Q000601:surgery; D008297:Male; D011817:Rabbits; D013927:Thrombosis / Q000628:therapy*