Protein-lipid interaction on the surface of a hydrophilic contact lens in vitro.
OBJECTIVE
To investigate the mechanism of protein-lipid interactions responsible for biofilm formation on the surface of hydrophilic contact lenses in vitro.


METHODS
New, never-worn hydrophilic contact lenses were individually incubated in a protein-only, lipid-only, or combination protein-lipid artificial tear solution for 24 hours at 37 degrees C with constant stirring. Deposited lipids were removed with a methanol based extraction procedure, separated using high-performance, thin layer chromatography and quantitatively analyzed densitometrically. Deposited proteins were extracted with 4M urea, separated using gel electrophoresis and quantitatively analyzed densitometrically.


RESULTS
The presence of protein in the artificial tear solution has a profound effect on the nature of lipid deposition for each group of hydrophilic lens, whereas the presence of lipid has a significant effect on the nature of protein deposition for only a group IV lens. In addition, the presence of lipid deposits on a group IV lens decreases the adsorption of lysozyme, while the presence of protein deposits reduces the amount of total lipid adhering to a group II lens.


CONCLUSIONS
Protein adsorption on a group IV lens renders the lens surface less hydrophilic and, thereby, more susceptible to lipid deposition, which in turn increases surface hydrophobicity and inhibits additional protein deposition. For a group II lens, positively charged protein competes with and replaces some of the polar lipids attached to the lens. Thus, the interaction of protein and lipid on a lens surface most prone to a particular contaminant apparently makes it less likely for that contaminant to bind.
Bontempo
A R
AR
Department of Biological Sciences, State University of New York, College of Optometry 10010, USA.
Rapp
J
J
D000327:Adsorption; D002855:Chromatography, Thin Layer; D003263:Contact Lenses, Hydrophilic; D066298:In Vitro Techniques; D007781:Lactoferrin / Q000378:metabolism*; D050356:Lipid Metabolism; D009113:Muramidase / Q000378:metabolism*; D009883:Ophthalmic Solutions; D012709:Serum Albumin / Q000378:metabolism*
D009883:Ophthalmic Solutions; D012709:Serum Albumin; D009113:Muramidase; D007781:Lactoferrin