Biotechnological potential of P450 monooxygenases high-level production of bovine cytochrome P450c17 monooxygenase during medium cell density culture of a recombinant yeast, Saccharomyces cerevisiae GRF 18 (YEp-Toku1).
Bovine cytochrome P450c17 monooxygenase was produced in a 30-L fermenter by Saccharomyces cerevisiae GRF18 (YEp-Toku1), harboring the GAL10 promoter, and using conditions of medium cell density culture. Upon addition of D-galactose as an inducer and FeCl3 as a cofactor, cells began to produce the P450 hemoprotein. The yield of this enzyme reached a maximum after 31 h but its formation continued for more than 60 h after induction. The amount of P450c17 produced was 4.7-fold as compared to shake flask experiments.
Nishihara
H
H
Institute of Technical Biochemistry, University of Stuttgart, Germany.
Okamura
T
T
Schmid
R D
RD
Hauck
A
A
Reuss
M
M
D002452:Cell Count; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013254:Steroid 17-alpha-Hydroxylase / Q000096:biosynthesis*
D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System; D013254:Steroid 17-alpha-Hydroxylase