title: In vivo mucosal uptake, mucosal transfer and retention of iron in mice.
abstract: An improved and sensitive method for studying iron absorption in mice with alterations in body iron stores is described. Mice with varying iron status were given a double isotope-labelled test dose containing 59Fe and 51Cr as a non-absorbable indicator, via an oroesophageal needle. Using a whole-body counter it was possible to measure in vivo the initial mucosal iron uptake and long-term iron retention and to calculate mucosal iron transfer. A significant difference was demonstrated between normal and both anaemic and dietary iron-loaded mice with regard to the various steps of iron absorption. When mice were tested twice for iron absorption, the results were highly reproducible. In conjunction with other parameters, the method described is useful in studying the mechanism and the regulation of iron absorption in mice.
authors.affiliation: Department of Immunology, Eijkman-Winkler Institute, University Hospital Utrecht, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.forename: K J
authors.initials: KJ
authors.lastname: Wienk
authors.forename: M W
authors.initials: MW
authors.lastname: Schilham
authors.forename: H
authors.initials: H
authors.lastname: Clevers
authors.forename: M
authors.initials: M
authors.lastname: de Sousa
authors.forename: J J
authors.initials: JJ
authors.lastname: Marx
mesh_terms: D000042:Absorption; D018798:Anemia, Iron-Deficiency / Q000378:metabolism; D000818:Animals; D002860:Chromium Radioisotopes; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D006706:Homeostasis; D007413:Intestinal Mucosa / Q000378:metabolism*; D007501:Iron / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D000090463:Iron Deficiencies; D007504:Iron Radioisotopes; D019266:Iron, Dietary / Q000008:administration & dosage / Q000493:pharmacokinetics; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D012727:Sex Characteristics; D014168:Transferrin / Q000032:analysis; D014915:Whole-Body Counting
chemical_list: D002860:Chromium Radioisotopes; D007504:Iron Radioisotopes; D019266:Iron, Dietary; D014168:Transferrin; D007501:Iron