HIV-1 envelope-specific CD4+ T helper cells from simian/human immunodeficiency virus-infected rhesus monkeys recognize epitopes restricted by MHC class II DRB1*0406 and DRB*W201 molecules.
The HIV-1 envelope (Env)-specific Th cell response of rhesus monkeys infected with nonpathogenic chimeric simian/human immunodeficiency viruses (SHIV) was assessed at a molecular level by establishing rgp120-specific CD4+ T cell lines. Epitopes recognized by these MHC class II-restricted Th cells were identified within the second (C2), third (C3), and fifth (C5) conserved regions of HIV-1 gp120. The epitope located in C2 was found to be restricted by the rhesus monkey (Macaca mulatta) leukocyte Ag Mamu-DRB1*0406, and the Th cell epitope in C5 was found to be restricted by Mamu-DRB*W201. The restriction element requirements of the C2 and C5 epitope-specific CD4+ Th cells appear to be rather stringent, because these peptide epitopes were not recognized in the presence of other Mamu-DR expressing cell lines. The ability to analyze HIV-1 Env-specific CD4+ T cell responses in SHIV-infected monkeys will enhance the utility of this model for studying AIDS pathogenesis and for assessing novel HIV-1 vaccine strategies.
Lekutis
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Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA. clekutis@bidmc.harvard.edu
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D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D059811:HLA-DRB1 Chains; D007371:Interferon-gamma / Q000096:biosynthesis; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000939:Epitopes; D015699:HIV Envelope Protein gp120; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma