Measurement of synthetic phenolic antioxidants in human tissues by high-performance liquid chromatography with coulometric electrochemical detection.
The antioxidants, 2-tert.-butyl-4-methoxyphenol (BHA) and its oxidative peroxidation product 2,2'-dihydroxy-3,3'-di-tert.-butyl-5,5'-dimethoxybiphenyl (di-BHA), 3,5-di-tert.-butyl-4-hydroxytoluene (BHT) and propyl gallate, were measured in plasma and tissue homogenates by HPLC and electrochemical detection, with a sensitivity down to 0.2 (BHA), 0.1 (di-BHA), 0.4 (BHT) and 1 (propyl gallate) ng ml(-1) of plasma or tissue homogenate. The data demonstrate that in man, at the current level of exposure to dietary antioxidants, significant amounts of BHA, BHT and propyl gallate are accumulated in the omentum. Furthermore, they provide the first evidence that the peroxidase-catalysed oxidation of BHA is operative in man.
Bianchi
L
L
Dipartimento di Farmacologia Preclinica e Clinica M. Aiazzi Mancini, Università degli Studi di Firenze, Italy.
Colivicchi
M A
MA
Della Corte
L
L
Valoti
M
M
Sgaragli
G P
GP
Bechi
P
P
D000368:Aged; D000975:Antioxidants / Q000032:analysis*; D001774:Blood Chemical Analysis; D002083:Butylated Hydroxyanisole / Q000031:analogs & derivatives / Q000032:analysis; D002084:Butylated Hydroxytoluene / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009852:Omentum; D010537:Peritoneum / Q000737:chemistry; D011435:Propyl Gallate / Q000032:analysis / Q000097:blood; D012680:Sensitivity and Specificity
D000975:Antioxidants; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D011435:Propyl Gallate; C040022:2,2'-dihydroxy-3,3'-di-tert-butyl-5,5'-dimethoxydiphenyl