Expression of hsc 70, but not hsp 70, in human third molar dental pulp.
The constitutive (hsc 70) and inducible (hsp 70) isoforms of heat shock protein 70 are important members of the superfamily of stress related proteins that protect and promote the recovery of cells from physiological and pathologic stress. The goal of this study was to define the baseline expression of hsc 70 and hsp 70 in disease-free, minimally stressed human dental pulp of the adult 3rd molar. Immunolocalization demonstrated moderate to heavy staining intensity for hsc 70 in both the cytoplasm and nucleus of odontoblasts and fibroblasts comprising the human pulp. Endothelial and smooth muscle cells displayed weak to moderate immunoreactivity for hsc 70 in both the cytoplasm and nucleus. Schwann cells demonstrated only weak nuclear staining for hsc 70. No immunoreactivity for hsp 70 was observed in any cell type in human pulp. Western, northern, and RT-PCR analysis of pulp preparations confirmed the expression of hsc 70 mRNA and protein within components of the pulp. These results demonstrate that cells of the human pulp express, under conditions of minimal stimulation, a key component of the stress response protein superfamily. The expression of hsc 70 under conditions of minimal stress may provide pulp components an advantage in resisting cell injury when stress occurs.
Sens
D A
DA
Robert C. Byrd Health Sciences Center, Department of Pathology, West Virginia University, Morgantown 26506, USA.
McGuirt
J P
JP
Khan
W
W
Howell
R M
RM
Todd
J H
JH
D000328:Adult; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002470:Cell Survival; D004396:Coloring Agents; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D007124:Immunoenzyme Techniques; D008964:Molar, Third / Q000166:cytology / Q000378:metabolism*; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism; D009804:Odontoblasts / Q000378:metabolism / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D012583:Schwann Cells / Q000378:metabolism / Q000648:ultrastructure; D013312:Stress, Physiological / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D002352:Carrier Proteins; D004396:Coloring Agents; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human