title: Unfolding and inactivation of Penaeus penicillatus acid phosphatase during denaturation by guanidine hydrochloride.
abstract: The conformational changes of penaeus penicillatus acid phosphatase during denaturation in guanidine hydrochloride solutions were studied by following changes in the intrinsic fluorescence, ultraviolet difference absorption, and circular dichroism spectra. Inactivation of the enzyme in guanidine hydrochloride solutions were compared with unfolding of the enzyme molecule. The results show that the extent of unfolding in guanidine solutions measured by several different methods closely coincide with each other and that slightly lower concentrations of guanidine are required to bring about inactivation than are required to produce significant conformational changes of the enzyme molecule. At the same concentrations, the inactivation rate constants are markedly faster than the rate constants for unfolding of the enzyme. The above results suggest that the active sites of this enzyme display more conformational flexibility than the enzyme molecule as a whole.
authors.affiliation: Department of Biology, Xiamen University, People's Republic of China.
authors.forename: S L
authors.initials: SL
authors.lastname: Chen
authors.forename: Q X
authors.initials: QX
authors.lastname: Chen
authors.forename: P Z
authors.initials: PZ
authors.lastname: Yang
authors.forename: W J
authors.initials: WJ
authors.lastname: Qiu
authors.forename: L T
authors.initials: LT
authors.lastname: Wang
authors.forename: H M
authors.initials: HM
authors.lastname: Zhou
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry* / Q000187:drug effects; D000818:Animals; D001665:Binding Sites; D002942:Circular Dichroism; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D033561:Penaeidae / Q000201:enzymology*; D011487:Protein Conformation / Q000187:drug effects*; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D006146:Guanidines; D000135:Acid Phosphatase; D019791:Guanidine