title: A surface swab method for culturing Foley catheters assays the pericatheter (urethral) but not the urine (luminal) microbial population.
abstract: Assessment of the urethral flora in patients with indwelling bladder catheters is problematic in the presence of urinary tract infection (UTI). A new surface swab method that samples the external catheter surface without interference from contaminated luminal contents is described. In vitro, recovery of adherent bacteria from the external catheter surface by the surface swab method was proportional to the bacterial density as measured by a comparison scrape method. In a prospective longitudinal assessment of three chronically catheterized subjects with polymicrobial catheter-associated UTI, a conventional roll plate catheter culture method suggested substantial overlap between the urethral and urine microbial populations, possibly a result of contamination of catheter cultures by infected urine. In contrast, the surface swab method revealed little overlap between these floras, evidence suggesting a predominantly luminal (rather than meatal) route of UTI acquisition. The new surface swab method should prove useful in future studies of the pathogenesis and prevention of catheter-associated UTI.
authors.affiliation: Department of Medicine, University of Minnesota, Veterans Affairs Medical Center, Minneapolis 55417, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Johnson
authors.forename: D
authors.initials: D
authors.lastname: Dykstra
authors.forename: J J
authors.initials: JJ
authors.lastname: Brown
authors.forename: B
authors.initials: B
authors.lastname: Kringstad
authors.forename: J L
authors.initials: JL
authors.lastname: Pryor
mesh_terms: D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D013293:Enterococcus faecalis / Q000302:isolation & purification; D004866:Equipment Contamination; D004926:Escherichia coli / Q000302:isolation & purification; D006801:Humans; D014521:Urethra / Q000382:microbiology*; D014546:Urinary Catheterization / Q000295:instrumentation*; D014552:Urinary Tract Infections / Q000382:microbiology; D014556:Urine / Q000382:microbiology*