Heterogeneity of epidermal growth factor binding kinetics on individual cells.
Binding of fluorescein-conjugated epidermal growth factor (EGF) to individual A431 cells at 4 degrees C is measured by a quantitative fluorescence imaging technique. After background fluorescence and cell autofluorescence photobleaching corrections, the kinetic data are fit to simple models of one monovalent site and two independent monovalent sites, both of which include a first-order dye photobleaching process. Model simulations and the results from data analysis indicate that the one-monovalent-site model does not describe EGF binding kinetics at the single-cell level, whereas the two-site model is consistent with, but not proved by, the single-cell binding data. In addition, the kinetics of binding of fluorescein-EGF to different cells from the same coverslip often differ significantly from each other, indicating cell-to-cell variations in the binding properties of the EGF receptor.
Chung
J C
JC
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003-4505, USA.
Sciaky
N
N
Gross
D J
DJ
D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005452:Fluoresceins; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods; D008956:Models, Chemical; D013997:Time Factors; D014407:Tumor Cells, Cultured
D005452:Fluoresceins; D004815:Epidermal Growth Factor