Virus isolation from wild-caught mosquitoes during a Japanese encephalitis outbreak in Kerala in 1996.
Out of 5357 wild-caught mosquitoes in 163 pools tested for virus using antigen capture ELISA and an insect-bioassay (inoculation into Toxorhynchites splendens larvae and identification by IFA using JE virus-specific monoclonal antibody), 16 flavivirus isolations were made of which 12 (75%) were identified as JE virus. Of the 12 JE virus isolations, 7 were from Culex tritaeniorhynchus, 3 from Mansonia uniformis and 1 each from Ma. indiana and Anopheles subpictus. Four isolations from Mansonia species for the first time reported here are noteworthy.
Dhanda
V
V
Vector Control Research Centre, Pondicherry.
Thenmozhi
V
V
Kumar
N P
NP
Hiriyan
J
J
Arunachalam
N
N
Balasubramanian
A
A
Ilango
A
A
Gajanana
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D009033:Culicidae / Q000821:virology*; D004196:Disease Outbreaks; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000453:epidemiology*; D004797:Enzyme-Linked Immunosorbent Assay; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007194:India / Q000453:epidemiology
D000911:Antibodies, Monoclonal