title: Tetranectin, a trimeric plasminogen-binding C-type lectin.
abstract: Tetranectin, a plasminogen-binding protein belonging to the family of C-type lectins, was expressed in E. coli and converted to its native form by in vitro refolding and proteolytic processing. Recombinant tetranectin-as well as natural tetranectin from human plasma-was shown by chemical cross-linking analysis and SDS-PAGE to be a homo-trimer in solution as are other known members of the collectin family of C-type lectins. Biochemical evidence is presented showing that an N-terminal domain encoded within exons 1 and 2 of the tetranectin gene is necessary and sufficient to govern subunit trimerization.
authors.affiliation: Department of Molecular and Structural Biology, University of Aarhus, Denmark.
authors.forename: T L
authors.initials: TL
authors.lastname: Holtet
authors.forename: J H
authors.initials: JH
authors.lastname: Graversen
authors.forename: I
authors.initials: I
authors.lastname: Clemmensen
authors.forename: H C
authors.initials: HC
authors.lastname: Thøgersen
authors.forename: M
authors.initials: M
authors.lastname: Etzerodt
mesh_terms: D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D010920:Placenta; D010958:Plasminogen / Q000378:metabolism; D011247:Pregnancy; D011487:Protein Conformation; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012996:Solutions
chemical_list: D001798:Blood Proteins; D003432:Cross-Linking Reagents; D037102:Lectins; D037181:Lectins, C-Type; D011993:Recombinant Fusion Proteins; D012996:Solutions; C048648:tetranectin; D010958:Plasminogen