title: Evaluation of 99mTc-labeled modified serum albumin for tumor detection.
abstract: Serum albumin (SA) modified and labeled with 131I-tyramine N-1'-desoxysorbitol (131I-TDS) has been shown to localize in tumors [Sinn et al., (1990) Nucl. Med. Biol. Part B 17, 819-827]. We prepared similar TDS complexes labeled with 99mTc and evaluated their potential for tumor imaging. Derivatization of SA with TDS was optimized using cyanuric chloride or 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDAC) as coupling agents. A high TDS loading yield of 38 mol/mol SA was obtained with the latter reagent. Modified SA (8 and 38 mol TDS/mol SA) were labeled with 99mTc via the stannous reduction method and injected i.v. into EMT-6 tumor bearing mice. 125I-TDS-SA (8 mol 125I-TDS/mol SA) revealed a high tumor uptake of 10% ID/g at 3 h post-injection. The 99mTc-labeled SA and TDS-SA complexes lacked tumor specificity, instead TDS loading of SA resulted in increased liver/spleen uptake, suggesting colloid formation. This study confirms the potential of modified SA for tumor imaging but highlights the importance of choice of radioisotope, as well as site of attachment of the radiolabel to the modified SA for optimal tumor localization.
authors.affiliation: MRC Group in the Radiation Sciences, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
authors.forename: L M
authors.initials: LM
authors.lastname: Guerdoud
authors.forename: R
authors.initials: R
authors.lastname: Ouellet
authors.forename: J E
authors.initials: JE
authors.lastname: Van Lier
mesh_terms: D000818:Animals; D005069:Evaluation Studies as Topic; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging*; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D012709:Serum Albumin / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D012709:Serum Albumin; C107869:technetium Tc 99m serum albumin