title: The recognition of haemoglobin by antibodies raised for the immunoassay of beta-amyloid.
abstract: Canine and porcine cerebrospinal fluid (CSF) were fractionated by size exclusion chromatography and analysed by a luminescence enzyme linked immunosorbent assay (ELISA) configured to detect beta-amyloid. A peak of activity was observed in the CSF consistent with the molecular weight of beta-amyloid. When CSF contaminated with blood was analysed an additional peak of immunoreactivity at a higher molecular weight was observed. The peak of activity was found to be derived from cross-reactivity of the immunoglobulins employed in the ELISA with haemoglobin. These findings are discussed with reference to primary and structural sequence homology between beta-amyloid and haemoglobin from a number of species, the known properties of beta-amyloid and recent clinical reports.
authors.affiliation: SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK. paul_cutler-1@sbphrd.com
authors.forename: P
authors.initials: P
authors.lastname: Cutler
authors.forename: F
authors.initials: F
authors.lastname: Brown
authors.forename: P
authors.initials: P
authors.lastname: Camilleri
authors.forename: D
authors.initials: D
authors.lastname: Carpenter
authors.forename: A
authors.initials: A
authors.lastname: George
authors.forename: C
authors.initials: C
authors.lastname: Gray
authors.forename: M
authors.initials: M
authors.lastname: Haran
authors.forename: B
authors.initials: B
authors.lastname: Stewart
mesh_terms: D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006454:Hemoglobins / Q000276:immunology*; D006801:Humans; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal; D006454:Hemoglobins