Amperometric bienzymic sensor for aspartame.
An amperometric enzyme electrode for the determination of aspartame was developed by covalent immobilization of alcohol oxidase and alpha-chymotrypsin. A platinum based hydrogen peroxide electrode was used as the detector. Excellent sensitivity was obtained using batch, flow-through and flow injection methods with detection limits of 2 x 10(-7), 4 x 10(-7) and 10(-6) mol l-1, respectively. Different strategies for eliminating interfering compounds, including the introduction of an additional alcohol oxidase-catalase membrane and signal subtraction using an alcohol electrode, were employed. A recovery study on seven food samples was carried out and the results were satisfactory.
Compagnone
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D
Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Italy.
O'Sullivan
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D
Guilbault
G G
GG
D001218:Aspartame / Q000032:analysis*; D015374:Biosensing Techniques; D005504:Food Analysis / Q000379:methods; D013549:Sweetening Agents / Q000032:analysis*
D013549:Sweetening Agents; D001218:Aspartame