The significance of Ras guanine nucleotide exchange factor, son of sevenless protein, in renal cell carcinoma cell lines.
OBJECTIVE
The aim of the present study is to clarify the significance of the Ras guanine-nucleotide exchange reaction in the proliferation of human renal cell carcinoma cell lines.


METHODS
We examined the expression of human son of sevenless-1 (hSos-1) protein and the epidermal growth factor (EGF) receptor in human renal cell carcinoma cell lines by Western blot analysis. Additionally, a dominant negative H-ras mutant, N116Y, which is known to inhibit the Ras guanine-nucleotide exchange reaction, was transfected into these cell lines by lipofection.


RESULTS
Human renal cell carcinoma cell lines expressed much higher amounts of the EGF receptor and hSos-1 protein than normal kidney tissue. Moreover, the N116Y ras mutant could strongly suppress cellular proliferation in these cell lines.


CONCLUSIONS
Augmentation of the Ras guanine-nucleotide exchange reaction might be essential to the proliferation of human renal cell carcinoma cells.
Shinohara
N
N
Department of Urology, Hokkaido University School of Medicine, Sapporo, Japan.
Ogiso
Y
Y
Tanaka
M
M
Sazawa
A
A
Harabayashi
T
T
Koyanagi
T
T
D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D066246:ErbB Receptors / Q000235:genetics*; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D011506:Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured; D020663:ras Guanine Nucleotide Exchange Factors
D015852:Eukaryotic Initiation Factor-2; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D020663:ras Guanine Nucleotide Exchange Factors; D066246:ErbB Receptors