The structure of the capsular polysaccharide from a swarming strain of pathogenic Proteus vulgaris.
The structure was determined for the capsular polysaccharide (CPS) isolated from a swarming strain of Proteus vulgaris, CP2-96, which was obtained from the spleen of an infected mouse. The CPS was extracted from the cell pellet by hot water, precipitated with ethanol, and further purified by gel-permeation chromatography. The structure was established by glycosyl composition and linkage analyses, and by NMR spectroscopy. The sequence of the glycosyl residues was determined by a NOESY experiment. The CPS is composed of a tetrasaccharide repeating unit with the following structure: OAc [symbol: see text] 4 -->4)-beta-D-Glcp-(1-->3)-beta-D-GalpNAc-(1-->2)-alpha-D-Glcp-(1-->4)-al pha- D-GlcpA-(1-->.
Rahman
M M
MM
Complex Carbohydrate Research Center, University of Georgia, Athens 30602, USA.
Guard-Petter
J
J
Asokan
K
K
Carlson
R W
RW
D000818:Animals; D016667:Bacterial Capsules / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D011512:Proteus Infections / Q000382:microbiology; D011514:Proteus vulgaris / Q000737:chemistry* / Q000472:pathogenicity; D017421:Sequence Analysis; D013154:Spleen / Q000382:microbiology
D009005:Monosaccharides; D009844:Oligosaccharides