Dimorphic primers derived from intron 1 for use in the molecular typing of HLA-B alleles.
We have identified a dimorphic site in intron 1 of the HLA-B gene. Oligotyping was performed on about 3000 samples using primers derived from this dimorphic site in combination with a locus-specific primer derived from intron 3. The distribution of B-alleles bearing each of the dimorphic sequences was approximately equal. These primers were mutually exclusive and yielded approximately 50% of the heterozygous samples as apparently homozygous in PCR products. Intermediate and almost high-resolution oligotyping of HLA-B alleles was achieved using 35 and 63 hybridization probes, respectively. This dimorphic site will provide a useful tool for other PCR-based HLA-B typing approaches.
Cereb
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Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA. n-cereb@ski.mskcc.org
Yang
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SY
D000483:Alleles; D017931:DNA Primers; D015235:HLA-B Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007438:Introns; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015235:HLA-B Antigens