title: Increased apoptotic cells in bone marrow biopsies from patients with aplastic anaemia.
abstract: In order to investigate the involvement of apoptosis in the pathogenesis of aplastic anaemia (AA) we determined the proportion of apoptotic cells in paraffin-embedded bone marrow biopsies from patients with aplastic anaemia using an in situ TdT-catalysed DNA nick end labelling (TUNEL) staining method. A significant increase in the proportion of mononuclear apoptotic cells was demonstrated in biopsies from patients with aplastic anaemia (8.19 +/- 1.45%) when compared with controls (2.07 +/- 0.86%). These data support the view that apoptosis may play a role in the pathophysiology of bone marrow failure.
authors.affiliation: Department of Clinical Medicine, School of Medicine, Ribeirão Preto, São Paulo, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Callera
authors.forename: R P
authors.initials: RP
authors.lastname: Falcão
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000473:pathology*; D017209:Apoptosis / Q000502:physiology*; D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged