Habutobin splits the Arg16-Gly17 bond in the A alpha chain of rabbit fibrinogen.
We reported previously that habutobin, a thrombin-like enzyme from Trimeresurus flavoviridis venom, clotted only rabbit fibrinogen, whereas human, monkey, bovine, dog, rat and guinea-pig fibrinogens were unaffected. In the present study, we investigated the cleavage site of the rabbit A alpha chain by habutobin. The fibrinopeptide released by habutobin was identical to the fibrinopeptide A released by thrombin, and its amino acid sequence corresponded to A alpha 1-16 of rabbit fibrinogen. It was clarified therefore that habutobin cleaves the Arg16-Gly17 bond in the A alpha chain of rabbit fibrinogen.
Kinjoh
K
K
1st Department of Physiology, School of Medicine, University of the Ryukyus, Okinawa, Japan.
Kosugi
T
T
Nakamura
M
M
Hanashiro
K
K
Sunagawa
M
M
Tokeshi
Y
Y
Eguchi
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine; D002417:Cattle; D003435:Crotalid Venoms; D004285:Dogs; D005340:Fibrinogen / Q000737:chemistry / Q000187:drug effects*; D005998:Glycine; D006801:Humans; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D012697:Serine Endopeptidases / Q000494:pharmacology*
D003435:Crotalid Venoms; D005340:Fibrinogen; D001120:Arginine; D012697:Serine Endopeptidases; C078095:habutobin; D005998:Glycine