title: Analytical derivatizations of volatile and hydrophilic carbonyls from aqueous matrix onto a solid phase of a polystyrene-divinylbenzene macroreticular resin.
abstract: Extraction and derivatization of carbonyls to benzyloximes, pentafluorobenzyloximes or 2,4-dinitrophenylhydrazones is simplified and reaction times are substantially reduced by simultaneous sorption and derivatization from aqueous solution onto a solid phase. In this reaction a macroreticular polystyrene-divinylbenzene resin acts as a sorbent and catalyst to allow simultaneous extraction and derivatization of hydrophilic and lipophilic aldehydes and ketones from simple as well as complex matrices including plasma. Conversion to the 2,4-dinitrophenylhydrazones or pentafluorobenzyloximation at ambient temperature requires 10 and 20 min, respectively. These reaction conditions correspond to at least a 6-fold reduction in reaction times for derivatization of the reactive aldelhydes and a 36-72-fold reduction for preparation of derivatives for the slower reacting ketones.
authors.affiliation: Department of Pathology, McMaster University, Hamilton, Ont., Canada.
authors.forename: S M
authors.initials: SM
authors.lastname: Breckenridge
authors.forename: X
authors.initials: X
authors.lastname: Yin
authors.forename: J M
authors.initials: JM
authors.lastname: Rosenfeld
authors.forename: Y H
authors.initials: YH
authors.lastname: Yu
mesh_terms: D000447:Aldehydes / Q000097:blood / Q000737:chemistry*; D017130:Angioplasty; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006898:Hydroxylamines / Q000737:chemistry; D007475:Ion Exchange Resins; D007659:Ketones / Q000097:blood / Q000737:chemistry*; D017240:Mitochondrial Myopathies / Q000097:blood / Q000188:drug therapy; D010084:Oxidation-Reduction; D010091:Oximes / Q000737:chemistry; D010659:Phenylhydrazines / Q000737:chemistry; D011137:Polystyrenes; D014815:Vitamins / Q000627:therapeutic use
chemical_list: D000447:Aldehydes; D006898:Hydroxylamines; D007475:Ion Exchange Resins; D007659:Ketones; D010091:Oximes; D010659:Phenylhydrazines; D011137:Polystyrenes; D014815:Vitamins; C004787:2,4-dinitrophenylhydrazine; C017064:benzyloxyamine; C003771:Amberlite XAD-2 resin