gamma-Linolenic acid-containing diet attenuates bleomycin-induced lung fibrosis in hamsters.
Although bleomycin (BLM), an antineoplastic drug, is used in the treatment of a variety of tumors, the mechanism(s) that contribute to its induced lung injury and fibrosis are not fully elucidated. Since alterations in the levels of certain fatty acid metabolites have been associated with BLM-induced lung injury, we tested the effects of dietary gamma-linolenic acid (GLA)-containing evening primrose oil on BLM-induced morphological alterations in the hamster lung, the marked elevation of tissue hydroxyproline (a marker for collagen synthesis), and elevated generation of arachidonic acid metabolites (marker of inflammatory mediators). Our data revealed that after 14 d of dietary GLA-containing oil (i) BLM-induced elevation of lung hydroxyproline was suppressed (P < 0.05), (ii) the marked BLM-induced elevation of lung leukotriene B4 (LTB4) (a marker of polymorphanuclear generation of proinflammatory LTB4) was significantly suppressed (P < 0.05). The decrease in LTB4 was accompanied by marked elevations (P < 0.05) of lung prostaglandin E1 (PGE1) and 15-hydroxyeicosatrienoic acid (15-HETrE), both with known antiinflammatory properties. Taken together, data from these studies suggest that dietary GLA-containing oil contributes to tissue elevation of PGE1 and 15-HETrE, which in vivo may attenuate lung inflammation and fibrosis.
Ziboh
V A
VA
Department of Dermatology, School of Medicine, University of California-Davis 95616, USA.
Yun
M
M
Hyde
D M
DM
Giri
S N
SN
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001761:Bleomycin / Q000633:toxicity*; D006224:Cricetinae; D004042:Dietary Fats, Unsaturated / Q000627:therapeutic use*; D005228:Fatty Acids, Essential / Q000494:pharmacology; D006909:Hydroxyproline / Q000378:metabolism; D007975:Leukotriene B4 / Q000096:biosynthesis; D008041:Linoleic Acids; D008168:Lung / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D031665:Oenothera biennis; D010938:Plant Oils; D011658:Pulmonary Fibrosis / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D017965:gamma-Linolenic Acid / Q000627:therapeutic use*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000903:Antibiotics, Antineoplastic; D004042:Dietary Fats, Unsaturated; D005228:Fatty Acids, Essential; D008041:Linoleic Acids; D010938:Plant Oils; D001761:Bleomycin; D007975:Leukotriene B4; C028498:evening primrose oil; D017965:gamma-Linolenic Acid; D006909:Hydroxyproline