Effects of transforming growth factor-beta on the production of parathyroid hormone-related peptide in a human ovarian cancer cell line in vitro.
OBJECTIVE
We examined the effects of transforming growth factor-beta 1 (TGF-beta 1) on intracellular parathyroid hormone-related peptide (PTHrP) production in a human ovarian cancer cell line (HOC-21).


METHODS
Various concentrations of TGF-beta 1 were added to a culture medium, and the numbers of cells were counted. HOC-21 cells were cultured with or without TGF-beta 1, and intracellular PTHrP was measured by a radioimmunoassay kit that recognized the carboxy-terminal protein while counting the number of cells. Furthermore, a 125I-TGF-beta 1 binding assay was carried out. Values were analyzed statistically using an analysis of variance followed by an unpaired t-test.


RESULTS
Five ng/ml of TGF-beta 1 inhibited the cell growth on Day 1 after plating. The TGF-beta 1 significantly (p < 0.05) stimulated intracellular PTHrP production in a time-dependent manner, also by Day 1. A 125I-TGF-beta 1 binding study revealed that HOC-21 cells expressed a high affinity for the TGF-beta 1 receptor.


CONCLUSIONS
The suppression of human ovarian cancer cell proliferation by TGF-beta 1 might be involved in the production of PTHrP.
Yasui
T
T
Department of Obstetrics and Gynecology, University of Tokushima School of Medicine, Japan.
Uemura
H
H
Irahara
M
M
Aono
T
T
D002455:Cell Division; D003470:Culture Media; D018283:Cystadenocarcinoma, Papillary / Q000378:metabolism; D018284:Cystadenocarcinoma, Serous / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000378:metabolism*; D044162:Parathyroid Hormone-Related Protein; D014176:Protein Biosynthesis; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D003470:Culture Media; C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D016212:Transforming Growth Factor beta