title: Effects of secretion inhibitors on the production of CAMP factor from Streptococcus agalactiae.
abstract: Investigations of exopeptide secretion with inhibitors were performed to study the synthesis and release of CAMP factor in drug-treated growing cells of Streptococcus agalactiae. Besides a reduction in cell growth, membrane-active substances including cerulenin and neuroactive drugs, such as procaine, dibucaine and atropine, increased the CAMP factor activity in culture supernatant. Quinacrine and phenylmethylsulphonyl fluoride, inhibitors of exopeptide-releasing proteases, reduced the bacterial growth, but did not affect the differential rate of the CAMP factor release. Polyanethole sulphonic acid, an anticoagulant preventing cell wall autolysis, promoted cell growth, but caused approximately 40% reduction in the production of CAMP factor from growing cells of S. agalactiae.
authors.affiliation: Laboratoire de Microbiologie Expérimentale et Pharmaceutique, Faculté de Pharmacie de l'Université de Kinshasa, Kinshasa XI, République Démocratique du Congo.
authors.forename: N B
authors.initials: NB
authors.lastname: Takaisi-Kikuni
mesh_terms: D000779:Anesthetics, Local / Q000494:pharmacology; D001285:Atropine / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D002569:Cerulenin / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006460:Hemolysin Proteins; D009268:Nalidixic Acid / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology; D011796:Quinacrine / Q000494:pharmacology; D013292:Streptococcus agalactiae / Q000187:drug effects* / Q000378:metabolism; D013451:Sulfonic Acids / Q000494:pharmacology; D014105:Tosyl Compounds / Q000494:pharmacology
chemical_list: D000779:Anesthetics, Local; D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D004791:Enzyme Inhibitors; D006460:Hemolysin Proteins; D011108:Polymers; D013451:Sulfonic Acids; D014105:Tosyl Compounds; D002569:Cerulenin; D009268:Nalidixic Acid; C003614:4-toluenesulfonyl fluoride; C077115:poly(anetholesulfonic acid); D001285:Atropine; D011796:Quinacrine