Isolation of the transferrin receptor from human placenta.
The transferrin receptor (TFR) has been detected in tissues characterised by a high degree of proliferation. We have developed a procedure for isolating TFR from human placental tissues by affinity chromatography on transferrin-Sepharose. Using gel filtration and electrophoresis in 7% PAAG, it has been shown that the molecular mass of the protein is 180 kDa. The protein has a subunit structure and is made up of two identical subunits, 90 kDa each. The constant for the protein binding to transferrin is equal to 5 x 10(-9) M. The yield of the protein isolated by the novel procedure exceeds 5-fold that obtained by previously described methods.
Kanevsky VYu
Moscow Research Institute of a Medical Ecology, Russia.
Pozdnyakova
L P
LP
Katukov VYu
Severin
S E
SE
D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel; D004586:Electrophoresis / Q000379:methods; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D008970:Molecular Weight; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011990:Receptors, Transferrin / Q000302:isolation & purification* / Q000378:metabolism; D014168:Transferrin / Q000737:chemistry / Q000378:metabolism
D007457:Iodine Radioisotopes; D011990:Receptors, Transferrin; D014168:Transferrin