An exceptionally stable helix from the ribosomal protein L9: implications for protein folding and stability.
The ribosomal protein L9 has an unusual structure comprising two compact globular domains connected by a 34 residue alpha-helix. The middle 17 residues of the helix are exposed to solvent while the first seven pack against and form part of the N-terminal domain, and the last ten form part of the C-terminal domain. Here we report results which show that a peptide corresponding to the central helix of L9 is monomeric in aqueous solution and >85% helical at 1 degrees C and 68(+/-7)% helical at 25 degrees C. This is considerably more helical than any other protein fragment studied to date. Another peptide corresponding to the middle 17 residues of the helix is monomeric and is 41(+/-4)% helical at 1 degrees C. Because the central helix has high intrinsic stability the globular N and C-terminal domains will likely be stabilized by their interactions with the helix. Therefore, the stability of the two terminal domains should not be completely independent because both domains gain stability from a shared structural element, the central helix. Also, the ability of the central helix to form native-like structure in isolation highlights a potential role for the helix in the early stages of the folding process.
Kuhlman
B
B
Department of Chemistry, State University of New York at Stony Brook, Stony Brook, NY, 11794-3400, USA.
Yang
H Y
HY
Boice
J A
JA
Fairman
R
R
Raleigh
D P
DP
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D002021:Buffers; D002942:Circular Dichroism; D001411:Geobacillus stearothermophilus / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009025:Morpholines / Q000737:chemistry; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012269:Ribosomal Proteins / Q000737:chemistry*; D012996:Solutions; D055672:Static Electricity
D001426:Bacterial Proteins; D002021:Buffers; D009025:Morpholines; D012269:Ribosomal Proteins; D012996:Solutions; C049650:ribosomal protein L9; C008550:morpholinopropane sulfonic acid