A method to direct sequence cosmid LAWRIST16 clones.
We developed a new cycle condition method optimized for direct sequencing LAWRIST16 cosmid clones based on dye terminator and dye primer cycle sequencing chemistries. We report a direct comparison of the two most widely used sequencing polymerase enzymes (AmpliTaq FS and Thermosequenase). The sequencing data obtained is of high quality and we believe this method could be routinely used in large scale sequencing facilities.
Repetto
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Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milan, Italy.
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D003195:Computer Communication Networks; D003360:Cosmids / Q000737:chemistry* / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*
D009841:Oligonucleotides; D004259:DNA-Directed DNA Polymerase