title: The efficacy of different moisturizers on barrier recovery in hairless mice evaluated by non-invasive bioengineering methods. A model to select the potentially most effective product.
abstract: Moisturizers (emollients) are used frequently on normal and diseased skin. However, only few studies have examined their effects in dynamic situations and in more clinically relevant settings. We evaluated the effect of 4 commonly used products in a hairless mice model after acute skin barrier perturbation with acetone. The efficacy was evaluated by measurement of the transepidermal water loss (TEWL) and electrical conductance at various time intervals during barrier repair. The test products were compared with acetone-treated air-exposed controls allowed to recover otherwise normally and with a known irritant product, chlorhexidine cream 1%. Locobase was the most effective product in correcting barrier function and significantly improved barrier function during early stages of barrier recovery (< 6 h) without interfering with late stages of barrier recovery (> 6 h). The irritant control product, chlorhexidine cream 1%, delayed barrier recovery in the late stages. The model makes it possible to evaluate the combined effects of exogenous and endogenous components on barrier repair and to select the potentially most effective products before performing more cumbersome and time-consuming field studies.
authors.affiliation: Department of Dermatology, Odense University Hospital, Denmark.
authors.forename: C G
authors.initials: CG
authors.lastname: Mortz
authors.forename: K E
authors.initials: KE
authors.lastname: Andersen
authors.forename: L
authors.initials: L
authors.lastname: Halkier-Sørensen
mesh_terms: D000096:Acetone / Q000009:adverse effects; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002710:Chlorhexidine / Q000627:therapeutic use; D017453:Dermatitis, Irritant / Q000188:drug therapy* / Q000209:etiology; D003879:Dermatologic Agents / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004643:Emollients / Q000627:therapeutic use*; D005260:Female; D005712:Galvanic Skin Response / Q000187:drug effects; D051379:Mice; D008812:Mice, Hairless; D012867:Skin / Q000166:cytology; D012881:Skin Temperature / Q000502:physiology; D013997:Time Factors; D014870:Water Loss, Insensible / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D003879:Dermatologic Agents; D004643:Emollients; D000096:Acetone; D002710:Chlorhexidine