Elimination of intercellular junctional communication requires lower Ras(leu61) levels than stimulation of anchorage-independent proliferation.
One of the effects of transformation by a variety of factors is a decrease in gap junctional, intercellular communication (GJIC). To investigate the role of the Ras oncogene product in gap junction closure, and to incorporate GJIC into the gamut of transformation-related properties which can be regulated by increasing levels of oncogene expression, a panel of murine C3H10T1/2 and 3T3L1 fibroblasts was constructed in which graded increments of Ras(leu61) could reliably be obtained. The inducibly activated Ras(leu61) protein substantially reduced or eliminated GJIC at levels much lower than those needed for neoplastic transformation, as indicated by acquisition of the ability to proliferate in the absence of anchorage. These results indicate that disruption of GJIC, although necessary, is not sufficient for neoplastic transformation in this system.
Brownell
H L
HL
Department of Microbiology, Queen's University, Kingston, Ontario, Canada.
Whitfield
J F
JF
Raptis
L
L
D016475:3T3 Cells; D000818:Animals; D002450:Cell Communication; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D017629:Gap Junctions / Q000502:physiology*; D007365:Intercellular Junctions / Q000502:physiology*; D051379:Mice; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D015398:Signal Transduction
D016283:Proto-Oncogene Proteins p21(ras)