Diagnosis of viral hepatitis with a nonisotopic hybridization assay.
The DNA enzyme immunoassay is an efficient method for the screening of PCR products derived from different hepatitis virus genomes, and allows to bypass both agarose gel electrophoresis and Southern blot hybridization with radioactively labeled probes. A wider application of this method will disclose new perspectives for the introduction of PCR in clinical laboratories.
Cariani
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E
Consorzio per le Biotecnologie, Consiglio Nazionale delle Ricerche (CNR), Brescia, Italy.
Ravaggi
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A
Zonaro
A
A
Albertini
A
A
Primi
D
D
D015139:Blotting, Southern; D004587:Electrophoresis, Agar Gel; D006525:Hepatitis, Viral, Human / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007553:Isotope Labeling; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*
D015345:Oligonucleotide Probes