Manual manufacturing of oligonucleotide, DNA, and protein microchips.
A simple procedure for manufacturing microchips containing various gel-immobilized compounds is described. A gel photopolymerization technique is introduced to produce micromatrices of polyacrylamide gel pads (25 x 25 x 20 microm and larger) separated by a hydrophobic glass surface. A pin device for the manual application of a compound in solution onto the activated polyacrylamide gel pad for immobilization is described. Oligonucleotide, DNA, and protein microchips have been produced by this method and tested by hybridization and immunoanalysis monitored with a fluorescence microscope. The effect of the lengths of the immobilized oligonucleotides and the hybridized RNA and DNA on hybridization of the oligonucleotide microchips was evaluated. This method can also be used for manufacturing microchips containing a variety of other compounds.
Guschin
D
D
Joint Human Genome Program, Argonne National Laboratory, Illinios 60439, USA.
Yershov
G
G
Zaslavsky
A
A
Gemmell
A
A
Shick
V
V
Proudnikov
D
D
Arenkov
P
P
Mirzabekov
A
A
D000906:Antibodies; D000918:Antibody Specificity; D001709:Biotechnology / Q000379:methods*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D017423:Sequence Analysis, RNA / Q000295:instrumentation*
D000906:Antibodies; D017931:DNA Primers; D009841:Oligonucleotides; D011506:Proteins