Matrix metalloproteinase expression and production by alveolar macrophages in emphysema.
The aim of this study was to examine the hypothesis that alveolar macrophages represent a significant source of matrix-degrading proteinases in the emphysematous lung. Macrophages from bronchoalveolar lavage fluid of 10 patients with emphysema and 10 normal volunteers were maintained in vitro for 24 h and assessed semiquantitatively for mRNA transcript levels of the matrix metalloproteinases (MMPs) gelatinases A and B, macrophage metalloelastase (MME), and interstitial collagenase. Release of these MMPs into the culture medium and secretion of neutrophil elastaselike activity was also assessed. Elevated levels of mRNA transcripts for gelatinase B (p < 0.0005) and interstitial collagenase (p < 0.0005) were observed in macrophages from emphysematous patients. Increased collagenase (p < 0.01) and neutrophil elastaselike activities (p < 0.001) were also measured in conditioned medium from patient macrophages. With gelatinase B, complexed forms of the enzyme were secreted by patient but not by control macrophages. No difference in transcript levels of gelatinase A or MME was observed between patient and control samples, and neither enzyme was detected in macrophage-conditioned media from either group. These results directly demonstrate that alveolar macrophages from the emphysematous lung produce elevated quantities of matrix-degrading enzymes with both elastolytic and collagenolytic activities.
Finlay
G A
GA
Department of Medicine and Therapeutics, Woodview, University College Dublin, Belfield, Ireland.
O'Driscoll
L R
LR
Russell
K J
KJ
D'Arcy
E M
EM
Masterson
J B
JB
FitzGerald
M X
MX
O'Connor
C M
CM
D000328:Adult; D000368:Aged; D017364:Collagenases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018093:Gelatinases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019272:Leukocyte Elastase / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000201:enzymology*; D008297:Male; D053508:Matrix Metalloproteinase 12; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology; D011656:Pulmonary Emphysema / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics; D012016:Reference Values
D012333:RNA, Messenger; D019272:Leukocyte Elastase; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C507122:MMP12 protein, human; D053508:Matrix Metalloproteinase 12