Rat GST 8-8 is expressed predominantly in myeloid origin cells infiltrating the gravid uterus.
Previous studies from our laboratory have shown a relatively high expression of rGST8-8 in uterine tissues. This GST isozyme displays relatively high glutathione-peroxidase activity towards lipid-hydroperoxides and towards toxic 4-hydroxyalkenals generated from lipid peroxidation. Since the uterus is a unique organ, subject to oxidative stress due to infiltration by immune effector cells during gestation and because this infiltration is readily identifiable histologically, the studies reported herein were performed to localize the cell specific expression of rGST8-8 to determine whether immune effector cells infiltrating the pregnant rat uterus specifically expressed rGST8-8. A 75 bp end-radiolabeled cRNA probe was prepared from the full length mGSTA4-4 cDNA from the region which is highly homologous with rGST8-8. This cRNA probe was used for in situ hybridization studies to localize rGST8-8 in specific cell types of gravid rat uterus. Results of these studies indicate that this GST isozyme is selectively expressed in myeloid origin cells such as monocytes/macrophages, and neutrophils infiltrating the uterine endometrium and in vascular walls. Selective expression of rGST8-8 in the myeloid origin cells, which are known to generate higher levels of reactive oxygen species, suggests that this GST isozyme plays an important role in the protection mechanisms against lipid peroxidation.
Awasthi
S
S
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555-1067, USA.
Singhal
S S
SS
Srivastava
S K
SK
Chaubey
M
M
Piper
J T
JT
Zimniak
P
P
Yallampalli
C
C
Rajaraman
S
S
Awasthi
Y C
YC
D000818:Animals; D001854:Bone Marrow Cells; D005260:Female; D005982:Glutathione Transferase / Q000096:biosynthesis*; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis*; D008054:Lipid Peroxides / Q000378:metabolism; D018384:Oxidative Stress; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000201:enzymology*; D014599:Uterus / Q000166:cytology / Q000201:enzymology*
D007527:Isoenzymes; D008054:Lipid Peroxides; D005982:Glutathione Transferase