title: Constant infusion and bolus injection of stable-label tracer give reproducible and comparable fasting HGO.
abstract: We have investigated the reproducibility of fasting hepatic glucose output (HGO) estimates by use of isotope dilution methodology of stable-label tracers. Six normal subjects were studied on two occasions 1 wk apart. After an overnight fast, the subjects received a bolus injection of 7 mg/kg of [U-13C]glucose and, simultaneously, a primed constant infusion of 0.05 mg.kg-1.min-1 of [6,6(-2)H]glucose. The bolus injection provided one estimate of HGO (HGOBOL), and the constant infusion provided two estimates of HGO, namely, HGO at 2 h (HGOINF2) and HGO at 4 h (HGOINF4), both with the assumption of steady-state conditions. All estimates were similar in value; HGOBOL was highest, followed by HGOINF2 and HGOINF4 [2.30 +/- 0.11 (SE), 2.17 +/- 0.12, and 2.01 +/- 0.13 mg.kg-1.min-1]. The constant infusion gave highly reproducible results. In the case of HGOINF2, the within-subject coefficient of variation (CV) was only 3% compared with 5% of HGOINF4. The reproducibility of HGOBOL was comparable with the within-subject CV of 7%. We conclude that a constant infusion and a bolus injection of stable-label tracer give reproducible and comparable estimates of HGO.
authors.affiliation: Centre for Measurement and Information in Medicine, City University, London, United Kingdom.
authors.forename: R
authors.initials: R
authors.lastname: Hovorka
authors.forename: D J
authors.initials: DJ
authors.lastname: Eckland
authors.forename: D
authors.initials: D
authors.lastname: Halliday
authors.forename: S
authors.initials: S
authors.lastname: Lettis
authors.forename: C E
authors.initials: CE
authors.lastname: Robinson
authors.forename: P
authors.initials: P
authors.lastname: Bannister
authors.forename: M A
authors.initials: MA
authors.lastname: Young
authors.forename: A
authors.initials: A
authors.lastname: Bye
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D002247:Carbon Isotopes; D003903:Deuterium; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D007262:Infusions, Intravenous / Q000379:methods; D007275:Injections, Intravenous / Q000379:methods; D008099:Liver / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D011865:Radioisotope Dilution Technique; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D002247:Carbon Isotopes; D003903:Deuterium; D005947:Glucose