Successful reversal of diabetes by single donor isologous islet transplantation in a mouse model.
A method for isolating mouse islets which consistently gives a high yield with good purity is described. Using a bovine serum albumin gradient, the mean yield of islets per pancreas is 425 (SEM +/- 15) with a consistent purity of over 90%. Single donor to single recipient of islets transplanted under the renal capsule restores normoglycemia in the diabetic recipients within 2 to 5 days of transplantation.
Leow
C K
CK
Naffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, UK.
Gray
D W
DW
Morris
P J
PJ
D000818:Animals; D002417:Cattle; D002469:Cell Separation / Q000379:methods*; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D007515:Islets of Langerhans / Q000473:pathology; D016381:Islets of Langerhans Transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014185:Transplantation, Isogeneic