Pore-forming properties of proteolytically nicked staphylococcal alpha-toxin: the ion channel in planar lipid bilayer membranes.
Staphylococcal alpha-toxin is a single-chain protein with a molecular mass of 33.2 kDa, which can form large water-filled pores both in lipid bilayers and in erythrocyte membranes. Limited proteolysis of the purified toxin with proteinase K led to time-dependent changes of all the functional features of the channels formed by the toxin. Single-channel conductance in planar bilayers was decreased about threefold. The anion selectivity of the channel was replaced with cation selectivity and the asymmetry in the current-voltage relationship of the channel became more pronounced. At the same time the nicked toxin kept its full ability to form ion channels in lipid bilayers, although it lost a considerable part of its hemolytic activity. In planar bilayers and in erythrocyte membranes, the proteolytically nicked toxin actually formed channels with a slightly smaller diameter (approximately 1.2 times) than that formed by the native toxin. This decrease was not marked enough to explain changes in the biological effects of the nicked toxin. The change in channel selectivity induced by the cleavage is considered to be the major determinant of the changes in the biological effects of the nicked toxin.
Krasilnikov
O V
OV
Laboratory of Molecular Physiology, Institute of Physiology and Biophysics, Tashkent, Uzbekistan. kras@npd.ufpe.br
Merzlyak
P G
PG
Yuldasheva
L N
LN
Azimova
R K
RK
Nogueira
R A
RA
D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D019286:Endopeptidase K / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000378:metabolism; D006460:Hemolysin Proteins / Q000378:metabolism*; D006461:Hemolysis; D007473:Ion Channels / Q000502:physiology*; D008051:Lipid Bilayers / Q000378:metabolism*; D011817:Rabbits; D055672:Static Electricity
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; C021091:staphylococcal alpha-toxin; D019286:Endopeptidase K