Urine glycosaminoglycan concentrations in mucopolysaccharidosis VI-affected cats following bone marrow transplantation or leukocyte infusion.
Urinary glycosaminoglycan (GAG) concentrations were determined in nineteen normal cats (eleven kittens and eight adult cats), eighteen mucopolysaccharidosis VI (MPS VI)-affected untreated cats (ten kittens and eight adult cats), thirteen cats MPS VI-affected cats following bone marrow transplants (BMT), and two MPS VI-affected cats following intravenous infusion of leukocytes from normal cats. Mucopolysaccharidosis VI-affected cats treated with BMT had a precipitous decrease in urinary GAG by day 7 post-BMT, then a transient increase just prior to engraftment, followed by a sustained decrease to within, or near, the range of urinary GAG concentration established for normal cats. The pre-engraftment changes in urinary GAG excretion were reproduced by leukocyte infusion. After infusion of comparable members of normal peripheral blood leukocytes, a significant decrease in urinary GAG concentrations, specifically dermatan sulfate (DS), was seen with a nadir at day 5 post-infusion, followed by a return by day 9 to pre-infusion values. Post-engraftment, a continued low urinary GAG concentration with a specific decrease in DS can be utilized to document successful autologous engraftment in MPS VI-affected cats.
Dial
S M
SM
Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
Byrne
T
T
Haskins
M
M
Gasper
P W
PW
Rose
B
B
Wenger
D A
DA
Thrall
M A
MA
D000818:Animals; D016026:Bone Marrow Transplantation / Q000662:veterinary*; D002371:Cat Diseases / Q000628:therapy* / Q000652:urine*; D002415:Cats; D006025:Glycosaminoglycans / Q000652:urine*; D006085:Graft Survival; D017708:Leukocyte Transfusion; D009087:Mucopolysaccharidosis VI / Q000628:therapy / Q000652:urine / Q000662:veterinary*; D013997:Time Factors; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D006025:Glycosaminoglycans