Novel SnoRNAs from Naja naja atra (Taiwan cobra) and Bungarus multicinctus (Taiwan banded krait), form extended sequence complementarity to 5S rRNA.
During the mapping and sequencing of Naja naja atra cobrotoxin and cardiotoxin 4 genes, we have found that novel small nucleolar RNAs (snoRNAs) are encoded in the first intron of the two genes. The snoRNAs in Naja naja atra were amplified from the venom glands cDNA mixtures of Naja naja atra by reverse transcription-polymerase chain reaction using the primers designed from the first intronic sequences of cobrotoxin and cardiotoxin 4 genes. Likewise, the snoRNAs in Bungarus multicinctus were also amplified by the same primers. Comparison of these snoRNA genes reveals that the regions involved in binding to 5S rRNA are highly conserved among these genes, and form 12-nt and 15-nt tracts of complementarity to phylogenetically invariant sequences in eukaryotic 5S rRNAs. The box C sequence in these snoRNAs is consensus, however, variations with the sequence of box D motif are observed. The present study is the first case of intron-encoded snoRNAs contain extended regions of perfect complementarity to mature 5S rRNA.
Chang
L
L
Department of Biochemistry, Kaohsiung Medical College, Taiwan, Republic of China. lschang@mail.nsysu.edu.tw
Hong
E
E
D000818:Animals; D001483:Base Sequence; D017838:Bungarus; D004179:Cobra Cardiotoxin Proteins / Q000235:genetics*; D003039:Cobra Neurotoxin Proteins / Q000235:genetics*; D004546:Elapid Venoms / Q000737:chemistry; D017815:Elapidae; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D012341:RNA, Ribosomal, 5S / Q000737:chemistry*; D012342:RNA, Small Nuclear / Q000737:chemistry*; D017385:Sequence Homology
D004179:Cobra Cardiotoxin Proteins; D003039:Cobra Neurotoxin Proteins; D004546:Elapid Venoms; D012341:RNA, Ribosomal, 5S; D012342:RNA, Small Nuclear; D012313:RNA