title: Structural investigation of the lipopolysaccharide from Acinetobacter haemolyticus strain NCTC 10305 (ATCC 17906, DNA group 4).
abstract: The structure of the lipopolysaccharide (LPS) from Acinetobacter haemolyticus strain NCTC 10305 (DNA group 4) was elucidated by means of analytical chemistry, NMR spectroscopy and fast-atom-bombardment mass spectrometry. Several oligosaccharides were obtained after deacylation or successive de-O-acylation, dephosphorylation, reduction, and de-N-acylation of LPS. In the major fraction of the LPS, the core is attached to the lipid A through D-glycero-D-talo-2-octulopyranosonic acid (Ko), whereas in a minor fraction (<20%) Ko is replaced by 3-deoxy-D-manno-octulopyranosonic acid (Kdo). The structures of the phosphorylated carbohydrate backbones of these LPS fractions are [structure: see text] with Dha = 3-deoxy-D-lyxo-2-heptulosaric acid, Sug = sugar and is Ko in a major fraction and Kdo in a minor fraction. All sugar residues have the D-configuration and are present in the pyranose form. Mass spectrometry of de-O-acylated LPS revealed the presence of an additional hexose residue in minor amounts, the position and nature of which could not be identified.
authors.affiliation: Division of Medical and Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Germany.
authors.forename: E V
authors.initials: EV
authors.lastname: Vinogradov
authors.forename: S
authors.initials: S
authors.lastname: Müller-Loennies
authors.forename: B O
authors.initials: BO
authors.lastname: Petersen
authors.forename: S
authors.initials: S
authors.lastname: Meshkov
authors.forename: J E
authors.initials: JE
authors.lastname: Thomas-Oates
authors.forename: O
authors.initials: O
authors.lastname: Holst
authors.forename: H
authors.initials: H
authors.lastname: Brade
mesh_terms: D000150:Acinetobacter / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000032:analysis
chemical_list: D008070:Lipopolysaccharides; D009844:Oligosaccharides