title: Base-pairings within the RNA pseudoknot associated with the simian retrovirus-1 gag-pro frameshift site.
abstract: Frameshift and readthrough sites within retroviral messenger RNAs are often followed by nucleotide sequences that have the potential to form pseudoknot structures. In the work presented here, NMR methods were used to characterize the base-pairings and structural features of the RNA pseudoknot downstream of the gag-pro frameshift site of simian retrovirus type-1 (SRV-1) and a functional mutant of the SRV-1 pseudoknot. Evidence is presented that these pseudoknots contain two A-form helical stems of six base-pairs each, connected by two loops, in a classic H-type pseudoknot topology. A particularly interesting feature is that the shorter of the two connecting loops, loop 1, consists of only a single adenosine nucleotide that spans the major groove of stem 2. In this respect, the frameshift-associated pseudoknots are structurally similar to the pseudoknot within the gene 32 mRNA of bacteriophage T2, previously characterized by NMR methods. Despite having similar nucleotide sequences, the solvent exchange rates of the imino protons at the junction of the helical stems in the wild-type and mutant frameshifting pseudoknots differ from each other and from the bacteriophage T2 pseudoknot. The implications of this finding are discussed.
authors.affiliation: Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, 78712, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Du
authors.forename: J A
authors.initials: JA
authors.lastname: Holland
authors.forename: M R
authors.initials: MR
authors.lastname: Hansen
authors.forename: D P
authors.initials: DP
authors.lastname: Giedroc
authors.forename: D W
authors.initials: DW
authors.lastname: Hoffman
mesh_terms: D018965:Frameshifting, Ribosomal / Q000235:genetics*; D015750:Genes, gag / Q000235:genetics; D015751:Genes, pol / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009154:Mutation; D017900:Myoviridae / Q000737:chemistry; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics; D015301:Retroviruses, Simian / Q000737:chemistry*
chemical_list: D012367:RNA, Viral