Prepro-neuropeptide Y mRNA and NPY binding sites in human inferior vagal ganglia.
The inferior vagal ganglia contain the cell bodies of centrally projecting vagal afferent neurones. Using in situ hybridization-histochemistry with a combination of two antisense neuropeptide Y (NPY) oligonucleotides, we have demonstrated that a population of human inferior vagal perikarya express mRNA encoding prepro-NPY, the precursor of NPY. In vitro receptor autoradiography, using both [125I]Bolton Hunter-NPY ([125I]BH-NPY, 15 pM) and [125I]peptide YY ([125I]PYY, 25 pM), enabled visualization of NPY binding sites. Competition binding with NPY (1 microM), PYY (1 microM) and [Leu31,Pro34]NPY (100 nM), suggest that both Y1 and Y2 receptor subtypes are present on human vagal afferent neurones. These observations suggest a potential role for NPY in neuromodulation of vagal transmission in humans.
McLean
K J
KJ
Department of Pharmacology, Monash University, Clayton, Vic., Australia.
Jarrott
B
B
Lawrence
A J
AJ
D001345:Autoradiography; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism* / Q000494:pharmacology*; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000187:drug effects*; D013388:Succinimides / Q000494:pharmacology*; D014630:Vagus Nerve / Q000187:drug effects*
D009478:Neuropeptide Y; D011498:Protein Precursors; D012333:RNA, Messenger; D018013:Receptors, Neuropeptide; D013388:Succinimides; C022581:Bolton-Hunter reagent; C042084:preproneuropeptide Y