Nucleoside-nucleotide-free diet suppresses cytokine production and contact sensitivity responses in rats with trinitrobenzene sulphonic acid-induced colitis.
We examined the effects of dietary nucleoside-nucleotide mixture on synthesis of inflammatory cytokines, interleukin-8 and tumor necrosis factor-alpha, in sensitized and nonsensitized colitic rats. Sensitized and nonsensitized colitic rats that were fed a nucleoside-nucleotide mixture had greater colonic weight and macroscopic and microscopic damage scores than nucleoside-nucleotide-free sensitized and nonsensitized colitic rats. Increased colonic tumor necrosis factor-alpha and interleukin-8 concentrations were associated with increased colonic inflammation and ulceration in the nucleoside-nucleotide mixture-fed group. There was also increased ear thickness in the nucleoside-nucleotide mixture-fed sensitized and nonsensitized colitic rats, which correlated highly with increased tumor necrosis factor-alpha and interleukin-8 levels in the ear lobes. Nucleoside-nucleotide-free diets may suppress cytokine secretion, thereby reducing colonic damage and contact sensitivity responses in colitic rats.
Adjei
A A
AA
Department of Bacteriology, University of the Ryukyus, Okinawa, Japan.
Ameho
C K
CK
Harrison
E K
EK
Yamauchi
K
K
Kulkarni
A
A
Kawajiri
A
A
Yamamoto
S
S
D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000473:pathology*; D016207:Cytokines / Q000096:biosynthesis*; D003877:Dermatitis, Contact / Q000378:metabolism* / Q000473:pathology; D004032:Diet; D004431:Ear, External / Q000473:pathology*; D005260:Female; D016209:Interleukin-8 / Q000096:biosynthesis; D009705:Nucleosides / Q000008:administration & dosage; D009711:Nucleotides / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D014302:Trinitrobenzenesulfonic Acid; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D016209:Interleukin-8; D009705:Nucleosides; D009711:Nucleotides; D014409:Tumor Necrosis Factor-alpha; D014302:Trinitrobenzenesulfonic Acid