[Clinical value of the study of proliferation and cell activation antigens with flow cytometry in bladder cancer].
The proliferative rate of tumor cells is frequently proportional to their degree of aggressiveness. The objective of the present study was to determine the correlation between the expression of three antigens associated with proliferation and the stage and ploidy of bladder cancers. The reactivity of antibodies against the nuclear antigen Ki-67 and membrane antigens T43 and the epidermal growth factor receptor (EGFR) was studied by flow cytometry on a series of 35 clinical samples of superficial and infiltrating bladder cancer and as well as on 5 specimens of normal urothelial cells. A preferential expression of the T43 antigen was observed on invasive cancer. EGFR was less frequently expressed; however, seven of the nine positive samples were from invasive cancers. Ki67 on the other hand did not show any selective expression according to tumor stage. When comparing Ki-67 and T43 expression on individual tumor samples, a negative correlation was found in that no tumor strongly expressed both markers simultaneously. These results suggest that markers of proliferation and activation do not all measure the same tumor parameters. Together, they may provide prognostic information that may be useful in the follow-up of patients with bladder cancer.
Lacombe
L
L
Centre de recherche en cancérologie de l'Université Laval, Québec, Canada.
Gauthier
J
J
Lafleur
L
L
Fradet
Y
Y
D000954:Antigens, Surface / Q000032:analysis / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000096:biosynthesis*; D002295:Carcinoma, Transitional Cell / Q000276:immunology* / Q000473:pathology*; D002455:Cell Division; D066246:ErbB Receptors / Q000032:analysis / Q000096:biosynthesis*; D005434:Flow Cytometry; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis / Q000096:biosynthesis*; D008562:Membrane Glycoproteins / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000473:pathology*
D000954:Antigens, Surface; D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; D066246:ErbB Receptors
Intérêt clinique de l'étude d'antigènes de prolifération et d'activation cellulaire par cytométrie en flux dans le cancer de la vessie.