Media composition modulates excitatory amino acid-induced death of rat cerebellar granule cells.
This study examined the effects of maintaining cells in different media and the role of serum in glutamate and NMDA-induced neurotoxicity in rat cerebellar granule cells. Glutamate stimulated a concentration-dependent cell death with similar potency in cerebellar granule cells grown in BME and Neurobasal media without serum. However, the maximal cell death to glutamate and N-methyl-D-aspartate (NMDA) varied in the different media compositions. In the presence of serum, glutamate and NMDA-induced excitotoxicity was abolished, suggesting a factor(s) in serum which influences glutamate-receptor mediated death. The protective effect of serum could be overcome by chronic stimulation with high doses of glutamate. The glutamate-stimulated increase in intracellular calcium load was attenuated in the presence of serum, resulting from an elevated basal calcium level, suggesting an association between raised basal calcium and neuroprotection.
Wood
A M
AM
Division of Neuroscience, School of Biological Sciences, University of Manchester, UK.
Tiwari
P
P
Bristow
D R
DR
D000818:Animals; D019540:Area Under Curve; D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D003470:Culture Media; D016895:Culture Media, Serum-Free; D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D018698:Glutamic Acid / Q000633:toxicity*; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D008928:Mitochondria / Q000201:enzymology; D016202:N-Methylaspartate / Q000633:toxicity*; D010088:Oxidoreductases / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D003470:Culture Media; D016895:Culture Media, Serum-Free; D018690:Excitatory Amino Acid Agonists; D018698:Glutamic Acid; D016202:N-Methylaspartate; D010088:Oxidoreductases; D002118:Calcium