Staurosporine induces telophase arrest and apoptosis blocking mitosis exit in human Chang liver cells.
Staurosporine is a protein kinase inhibitor that is known to block G1/S and G2/M cell cycle transitions, resulting in the accumulation of G1 or G2 interphase cells, depending on the applied dosage. It is, however, not known to block mitosis (M-phase) progression. We show here that a 15 min prepulse with 500 nM staurosporine had caused mitotic cell arrest in human Chang liver cells, accumulating telophase cells distinguished by their doublet nuclei and cytokinetic constrictions. The arrested telophase cells perished by apoptosis. Telophase-specific cell cycle arrest and apoptosis are novel. Staurosporine targeting cytokinesis could provide insights into late mitosis where control mechanisms remain largely unknown.
Swe
M
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Department of Anatomy, Faculty of Medicine, National University of Singapore, Kent Ridge.
Sit
K H
KH
D017209:Apoptosis; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D016193:G1 Phase / Q000187:drug effects; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects*; D047428:Protein Kinase Inhibitors; D016196:S Phase / Q000187:drug effects; D019311:Staurosporine / Q000494:pharmacology*; D013692:Telophase
D004791:Enzyme Inhibitors; D047428:Protein Kinase Inhibitors; D004247:DNA; D019311:Staurosporine