Adaptation of the primer extension preamplification (PEP) reaction for preimplantation diagnosis: single blastomere analysis using short PEP protocols.
Primer extension preamplification (PEP) was first described as a method for whole genome amplification, starting from a single cell, originally a spermatozoon, in order to perform genetic recombination studies. Its usefulness for preimplantation diagnosis was shown soon after; the only drawback was the length of the procedure (> 14 h). We have developed a shorter PEP protocol for single human blastomeres, enabling us to examine several genetic loci of interest in human genetic diseases with a good amplification efficiency.
Sermon
K
K
Centre for Medical Genetics, Dutch-speaking Brussels Free University, Belgium.
Lissens
W
W
Joris
H
H
Van Steirteghem
A
A
Liebaers
I
I
D001757:Blastomeres / Q000502:physiology*; D003550:Cystic Fibrosis / Q000235:genetics; D017931:DNA Primers; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D013661:Tay-Sachs Disease / Q000235:genetics
D017931:DNA Primers