Development of an amperometric flow injection immunoanalysis system for the determination of the herbicide 2,4-dichlorophenoxyacetic acid in water.
An amperometric flow injection immunoanalysis (FIIA) system based on an immunoreactor with immobilized biocomponents on a silica surface has been developed for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). In the antigen coating mode the hapten was immobilized and monoclonal primary antibody against 2,4-D together with alkaline phosphatase (AP)-labelled secondary antibody were used as sensing elements in a titration assay. In the antibody coating mode a biotinylated monoclonal antibody was immobilized on the surface of the immunoreactor and a 2,4-D-AP-conjugate was used for detection. For electrochemical measurements p-aminophenol enzymatically generated from p-aminophenyl phosphate was oxidized at a carbon working electrode at +150 mV versus Ag/AgCl. The system enabled the determination of 2,4-D in drinking water samples in the range from 0.2 to 70 micrograms/l. The whole system was computer controlled with a measuring time of 12 min for one determination.
Trau
D
D
Institute of Chemical and Biochemical Sensor Research, ICB, Münster, Germany.
Theueri
T
T
Wilmer
M
M
Meusel
M
M
Spener
F
F
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis*; D000469:Alkaline Phosphatase; D000818:Animals; D000911:Antibodies, Monoclonal; D004306:Dose-Response Relationship, Immunologic; D004563:Electrochemistry; D006540:Herbicides / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000295:instrumentation*; D007124:Immunoenzyme Techniques; D051379:Mice; D014867:Water / Q000032:analysis; D014873:Water Pollutants / Q000032:analysis
D000911:Antibodies, Monoclonal; D006540:Herbicides; D014873:Water Pollutants; D014867:Water; D015084:2,4-Dichlorophenoxyacetic Acid; D000469:Alkaline Phosphatase