Effect of cisplatin alone and in combination with gamma-radiation on the initiation of DNA synthesis in Friend leukemia cells.
The effect of the anticancer drug cisplatin (alone and in combination with gamma-radiation) on the initiation of DNA synthesis in Friend leukemia cells was studied. A method for isolation of DNA fractions containing the origins of replication was used. It was found that cisplatin decreased the rate of the initiation of DNA synthesis. The mild gamma-radiation has previously been observed to inhibit the initiation of DNA synthesis. In the present investigation the combination of cisplatin and gamma-radiation showed additive effects without synergism on the initiation of DNA biosynthesis.
Ganeva
R L
RL
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Tzvetkov
L M
LM
D000818:Animals; D002945:Cisplatin / Q000633:toxicity*; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000187:drug effects / Q000528:radiation effects; D005622:Friend murine leukemia virus; D005720:Gamma Rays; D007700:Kinetics; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D013936:Thymidine / Q000378:metabolism; D014307:Trioxsalen / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D004273:DNA, Neoplasm; D002945:Cisplatin; D013936:Thymidine; D014307:Trioxsalen