The breast cancer gene product TSG101: a regulator of ubiquitination?
Sequence analysis is a powerful tool to obtain structural and functional information about genes and their products. Here we show that TSG101, a gene subjected to somatic mutations in breast cancer, contains an amino terminal domain that is a homologue of ubiquitin conjugating enzymes (UBCs) and not, as previously proposed, DNA-binding domains. As the UBC active site residue is replaced in the TSG101 sequence in a similar manner to several other members of the UBC family, we propose a role for TSG101 in regulating the ubiquitination of short-lived gene products.
Ponting
C P
CP
University of Oxford, Fibrinolysis Research Unit, UK.
Cai
Y D
YD
Bork
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics*; D002645:Chickens; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D056827:Endosomal Sorting Complexes Required for Transport; D005260:Female; D006801:Humans; D008025:Ligases / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D044763:Ubiquitin-Conjugating Enzymes; D014452:Ubiquitins / Q000378:metabolism*; D000099177:Tsg101 Protein
D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D008025:Ligases; D014157:Transcription Factors; D044763:Ubiquitin-Conjugating Enzymes; D014452:Ubiquitins; D000099177:Tsg101 Protein