[Participation of Campylobacter jejuni flagellar epitopes in cellular adhesion].
Using a flagellated (052) and an aflagellated (T-1) strains we studied the participation of flagellar epitopes of C. jejuni in the adhesion to HEp-2 cells in vitro. Strain 052 was significantly more adherent than strain T-1. When adhesion assays were carried out with antiflagella monoclonal antibodies, strain 052 showed inhibition of their adhesive capacity that varied between 64.3 and 92.9%. With an ELISA test it was demonstrated that those monoclonal antibodies were specific and directed exclusively against flagellar epitopes of strain 052 being unreactive with strain T-1. Our results show that flagellar epitopes could participate in the adhesion process suggesting that flagella could be involved in the installation of the infectious process.
Andrews
E
E
Departamento de Microbiología, Universidad de Concepción, Valdivia, Chile.
Fernández
H
H
Folch
H
H
Eller
G
G
Pollette
M
M
D000911:Antibodies, Monoclonal / Q000276:immunology; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000302:isolation & purification / Q000472:pathogenicity / Q000648:ultrastructure*; D002448:Cell Adhesion; D000939:Epitopes / Q000276:immunology*; D005407:Flagella / Q000276:immunology*; D006801:Humans; D007223:Infant; D008297:Male; D008854:Microscopy, Electron
D000911:Antibodies, Monoclonal; D000939:Epitopes
Participación de epitopes flagelares de Campylobacter jejuni en la adhesion celular.