Lipopolysaccharide decreases oxygen consumption by Mono Mac 6 cells; an electron paramagnetic resonance oximetry study.
The rate of oxygen consumption in the human acute monocytic leukemia-derived cell line, Mono Mac 6, in response to lipopolysaccharide (LPS) in vitro was measured by electron paramagnetic resonance spectroscopy using an oxygen-sensitive spin-label, 4-oxo-2,2,6,6-tetramethylpiperidine-d16-1-oxyl (15N-PDT). Lipopolysaccharide impaired oxygen consumption in a dose-dependent manner which was shown to be mediated by mitochondrial dysfunction and could be augmented by pretreatment of the cells with interferon-gamma. Treatment of the cells with anti-CD14 monoclonal antibody failed to inhibit the LPS-induced effects on cellular respiration. These results suggest that LPS can directly reduce normal cellular oxygen consumption possibly via a CD14-independent pathway. This alteration of mitochondrial function by LPS may be responsible for the observed cell damage during sepsis.
Glover
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RE
Department of Medical Microbiology, University of Wales, College of Medicine, Cardiff, UK.
Mile
B
B
Rowlands
C C
CC
Jackson
S K
SK
D015536:Down-Regulation; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010092:Oximetry; D010101:Oxygen Consumption / Q000187:drug effects*; D013113:Spin Labels; D014407:Tumor Cells, Cultured
D008070:Lipopolysaccharides; D013113:Spin Labels; D007371:Interferon-gamma