title: Glycosphingolipid antigens from Leishmania (L.) amazonensis amastigotes. Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo.
abstract: Specific glycosphingolipid antigens of Leishmania (L.) amazonensis amastigotes reactive with the monoclonal antibodies (MoAbs) ST-3, ST-4 and ST-5 were isolated, and their structure was partially elucidated by negative ion fast atom bombardment mass spectrometry. The glycan moieties of five antigens presented linear sequences of hexoses and N-acetylhexosamines ranging from four to six sugar residues, and the ceramide moieties were found to be composed by a sphingosine d18:1 and fatty acids 24:1 or 16:0. Affinities of the three monoclonal antibodies to amastigote glycosphingolipid antigens were also analyzed by ELISA. MoAb ST-3 reacted equally well with all glycosphingolipid antigens tested, whereas ST-4 and ST-5 presented higher affinities to glycosphingolipids with longer carbohydrate chains, with five or more sugar units (slow migrating bands on HPTLC). Macrophages isolated from footpad lesions of BALB/c mice infected with Leishmania (L.) amazonensis were incubated with MoAb ST-3 and, by indirect immunofluorescence, labeling was only detected on the parasite, whereas no fluorescence was observed on the surface of the infected macrophages, indicating that these glycosphingolipid antigens are not acquired from the host cell but synthesized by the amastigote. Intravenous administration of 125I-labeled ST-3 antibody to infected BALB/c mice showed that MoAb ST-3 accumulated significantly in the footpad lesions in comparison to blood and other tissues.
authors.affiliation: Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, Brasil. straus.bioq@epm.br
authors.forename: A H
authors.initials: AH
authors.lastname: Straus
authors.forename: V B
authors.initials: VB
authors.lastname: Valero
authors.forename: C M
authors.initials: CM
authors.lastname: Takizawa
authors.forename: S B
authors.initials: SB
authors.lastname: Levery
authors.forename: M S
authors.initials: MS
authors.lastname: Toledo
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: M E
authors.initials: ME
authors.lastname: Salyan
authors.forename: S
authors.initials: S
authors.lastname: Hakomori
authors.forename: C L
authors.initials: CL
authors.lastname: Barbieri
authors.forename: H K
authors.initials: HK
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D006028:Glycosphingolipids / Q000276:immunology*; D007894:Leishmania mexicana / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D006028:Glycosphingolipids