Decreased kidney uptake of technetium-99m-labelled Fab' fragments in ovarian carcinoma bearing nude mice using a cleavable chelator.
A new 99mTc labelling method using a cleavable chelator, RP-1, was developed. In this study Balb/c mice with ovarian carcinoma xenografts received various Fab' fragments labelled with 99mTc either directly or via RP-1. Kidney uptake was significantly lower for the RP-1 linked conjugates. Tumour uptake showed no significant differences between RP-1 conjugates and directly labelled preparations. In conclusion, with the use of the cleavable linker RP-1, kidney uptake can be reduced significantly resulting in a lower radiation dose to the kidneys.
Tibben
J G
JG
Department of Obstetrics and Gynaecology, University Hospital Nijmegen, The Netherlands.
Massuger
L F
LF
Boerman
O C
OC
Claessens
R A
RA
Borm
G F
GF
Pak
K Y
KY
Koenders
E B
EB
Corstens
F H
FH
D000818:Animals; D019540:Area Under Curve; D002614:Chelating Agents; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D010051:Ovarian Neoplasms / Q000378:metabolism*; D011829:Radiation Dosage; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D002614:Chelating Agents; D007140:Immunoglobulin Fab Fragments; D015609:Organotechnetium Compounds