Preclinical evaluation and PET imaging of 18F-labeled Mel-14 F(ab')2 fragment in normal dogs.
The F(ab')2 fragment of monoclonal antibody Mel-14, reactive with human melanomas and gliomas, was labeled with 18F using two acylation agents, N-succinimidyl 8-[(4'-[18F]fluorobenzyl)amino]suberate (SFBS) and N-succinimidyl 4-[18F]fluorobenzoate (SFB). The immunoreactivity and affinity for Mel-14 F(ab')2 labeled using the two methods were similar. As a prelude to human clinical evaluation, PET imaging, tissue distribution and pharmacokinetic measurements were performed in two groups of normal foxhounds. Similar in vivo behavior was seen for Mel-14 F(ab')2 labeled using SFBS and SFB. Radiation dosimetry calculations suggest that a 10 mCi dose could be used for this F(ab')2 fragment labeled using either acylation agent.
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North Carolina State University College of Veterinary Medicine, Raleigh 27606, USA.
Garg
P K
PK
Vaidyanathan
G
G
Zalutsky
M R
MR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D004285:Dogs; D005462:Fluorine Radioisotopes; D005464:Fluorobenzenes / Q000493:pharmacokinetics*; D006207:Half-Life; D007668:Kidney / Q000000981:diagnostic imaging / Q000378:metabolism; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism; D008168:Lung / Q000000981:diagnostic imaging / Q000378:metabolism; D011829:Radiation Dosage; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed
D000911:Antibodies, Monoclonal; D005462:Fluorine Radioisotopes; D005464:Fluorobenzenes; D019275:Radiopharmaceuticals