Simplification of DNA topology below equilibrium values by type II topoisomerases.
Type II DNA topoisomerases catalyze the interconversion of DNA topoisomers by transporting one DNA segment through another. The steady-state fraction of knotted or catenated DNA molecules produced by prokaryotic and eukaryotic type II topoisomerases was found to be as much as 80 times lower than at thermodynamic equilibrium. These enzymes also yielded a tighter distribution of linking number topoisomers than at equilibrium. Thus, topoisomerases do not merely catalyze passage of randomly juxtaposed DNA segments but control a global property of DNA, its topology. The results imply that type II topoisomerases use the energy of adenosine triphosphate hydrolysis to preferentially remove the topological links that provide barriers to DNA segregation.
Rybenkov
V V
VV
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Ullsperger
C
C
Vologodskii
A V
AV
Cozzarelli
N R
NR
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D027101:DNA Topoisomerase IV; D004250:DNA Topoisomerases, Type II / Q000737:chemistry / Q000378:metabolism*; D004270:DNA, Circular / Q000737:chemistry / Q000378:metabolism*; D004278:DNA, Superhelical / Q000737:chemistry / Q000378:metabolism*; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D006868:Hydrolysis; D008956:Models, Chemical; D017900:Myoviridae / Q000201:enzymology; D009690:Nucleic Acid Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013816:Thermodynamics
D004270:DNA, Circular; D004278:DNA, Superhelical; D004279:DNA, Viral; D000255:Adenosine Triphosphate; D027101:DNA Topoisomerase IV; D004250:DNA Topoisomerases, Type II