Study of a lanthanide fluorescence system with a coupled reaction based on hemin catalysis.
The oxidation reactions of three substrates, p-hydroxybenzoic acid (HBA), p-hydroxyphenylacetic acid, and p-hydroxyphenylpropionic acid, with H2O2 catalysed by hemin were studied. It was significant that only the oxidation product of HBA, di-p,p'-hydroxybenzoic acid, forms a long-lived fluorescent complex with Tb3+. The three substrates themselves do not form fluorescent complexes with Tb3+ although they have chemical structural similarities. Based on this, a novel lanthanide fluorescence system with a coupled reaction was developed. The possible reactive mechanism is discussed. HBA was concluded to be well suited to several types of application from hemin determination to bovine serum albumin labeling.
Zheng
X Y
XY
Department of Chemistry, Xiamen University, China.
Lu
J Z
JZ
Zhu
Q Z
QZ
Xu
J G
JG
Li
Q G
QG
D002384:Catalysis; D015200:Fluoroimmunoassay / Q000379:methods*; D006427:Hemin; D008674:Metals, Rare Earth; D012710:Serum Albumin, Bovine
D008674:Metals, Rare Earth; D012710:Serum Albumin, Bovine; D006427:Hemin