title: Expression of sialosyl-T and disialosyl-T antigens in erythroid cells.
abstract: Expression of T, sialosyl-T and disialosyl-T antigens on normal blood and bone marrow cells as well as transformed cells was examined using specific monoclonal antibodies and multidimensional flow cytometry. Both anti-sialosyl-T (QSH1) and anti-disialosyl-T (QSH2) monoclonal antibodies aggregated erythrocytes. The anti-disialosyl-T antibody was specific for the erythroid lineage and did not react with neutrophils, monocytes or T-lymphocytes, while the anti-sialosyl-T antibody reacted with erythroid cells and a subset of T-lymphocytes. The developing erythroid cells in bone marrow showed coordinate expression of glycophorin A and the two carbohydrate chains, sialosyl-T and disialosyl-T. Analysis of neoplastic cells showed that the anti-disialosyl-T antibody only reacted with glycophorin A-positive blasts from erythroleukemia (FAB M6) patients (4/4) and one patient with chronic myeloid leukemia in erythroblastic transformation (CMLET). Leukemic blasts from these patients demonstrated coordinate quantitative expression of glycophorin A and disialosyl-T. The anti-sialosyl-T antibody reacted with glycophorin A-positive blasts from FAB M6 patients (4/4) and one CMLET patient; however, the antibody also reacted with glycophorin A-negative blasts from one FAB M6 and the one CMLET patients and transformed cells from other types of leukemia. The anti-T monoclonal antibody (HH8) did not react with any of the other cells tested. These results indicate that glycophorin A and disialosyl-T expression are tightly linked during normal erythroid development and erythroid leukemogenesis.
authors.affiliation: Division of Transfusion Medicine, Jichi Medical School, Minamikawachi-machi, Tochigi-ken, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Muroi
authors.forename: Y
authors.initials: Y
authors.lastname: Amemiya
authors.forename: E L
authors.initials: EL
authors.lastname: Sievers
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: S I
authors.initials: SI
authors.lastname: Hakamori
authors.forename: M R
authors.initials: MR
authors.lastname: Loken
mesh_terms: D000954:Antigens, Surface / Q000097:blood / Q000378:metabolism*; D002240:Carbohydrate Sequence; D004912:Erythrocytes / Q000378:metabolism*; D006021:Glycophorins / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000378:metabolism*; D008969:Molecular Sequence Data; D009077:Mucins / Q000097:blood / Q000378:metabolism*; D009844:Oligosaccharides / Q000097:blood / Q000378:metabolism*
chemical_list: D000954:Antigens, Surface; D006021:Glycophorins; D009077:Mucins; D009844:Oligosaccharides; C108031:disialosyl-T antigen, human; C087769:sialosyl-T antigen