Bovine IgG2a antibodies to Haemophilus somnus and allotype expression.
Bovine IgG2a has been implicated in protection against pyogenic infections, including those caused by Haemophilus somnus. To further investigate the role of IgG2a in defense against H. somnus, IgG1 and IgG2a antibodies were purified from antiserum against an immunodominant 40 kDa outer membrane protein (p40) of H. somnus, which was previously shown to passively protect calves against H. somnus pneumonia. The passive protective capacity of anti-p40 IgG1 or IgG2a was evaluated in vivo in calves. Purified anti-p40 IgG1 or IgG2a was incubated with H. somnus for 15 min before intrabronchial inoculation of calves. Bacteria incubated with anti-p40 IgG1 or IgG2a were inoculated into one caudal lung lobe and bacteria incubated with IgG1 or IgG2a from the respective preimmunization serum were inoculated into the contralateral lobe. The volumes of pneumonia in the right and left lungs were determined 24 h later. The difference in volume of pneumonia with H. somnus preincubated in IgG1 pre- and postimmunization anti p40 was less (16 cm3, P = 0.298) than the difference in volume of pneumonia with H. somnus preincubated in IgG2a pre- and postimmunization anti p40 (30 cm3, P = 0.146). Although the differences in lesion size between pre- and postimmunization serum were not statistically significant, the trend suggests IgG2a may be more protective than IgG1. To examine this further, the peptide specificity of these IgG1 and IgG2a antibodies to p40 was examined. After limited proteolysis of p40, IgG2a antibodies reacted with 2 peptides not recognized by IgG1 antibodies. Other peptides were recognized by both isotypes. Since these studies suggested that IgG2a may be important in protection against infection, we then investigated some aspects of the role of the 2 IgG2a allotypes, A1 and A2. In retrospective studies of age differences in expression of IgG2a allotypes, no heterozygotes were detected in calves of 60 d old or less, and fewer heterozygotes were detected in calves 61-120 d old than in cattle older than 270 d (P < 0.01). In a subsequent prospective study of the time course of allotype expression, Holstein calves shown to be heterozygotes expressed the IgG2aA1 allotype early but the IgG2aA2 allotype was not usually detected until 3 to 4 mo of age. Thus, both the retrospective and the prospective studies showed age related differences in expression of the IgG2aA1 and A2 allotypes. This could have implication in protection.
Corbeil
L B
LB
Department of Pathology, University of California, San Diego 92103-8416, USA.
Gogolewski
R P
RP
Kacskovics
I
I
Nielsen
K H
KH
Corbeil
R R
RR
Morrill
J L
JL
Greenwood
R
R
Butler
J E
JE
D000367:Age Factors; D000483:Alleles; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D015153:Blotting, Western / Q000379:methods / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D005260:Female; D006190:Haemophilus / Q000276:immunology*; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D006579:Heterozygote; D007116:Immunization, Passive / Q000662:veterinary; D007126:Immunoglobulin Allotypes / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood / Q000276:immunology; D008168:Lung / Q000473:pathology; D011014:Pneumonia / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary; D011446:Prospective Studies; D012189:Retrospective Studies
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G