Differential action of AGCF2 upon cell type-dependent expression of human angiotensinogen gene.
To investigate the regulatory mechanisms of human angiotensinogen (ANG) gene expression in the brain, we analyzed the 1.3-kb promoter by transfection studies and gel shift assays. The region from -106 to +44 was sufficient for promoter activity in glioblastoma cells, and multiple nuclear factors including AGCF2 (human ANG core promoter binding factor 2) bound within this 150-bp region. The mutations within AGCF2-binding elements decreased the transcriptional activity in glioblastoma cells but rather increased it in hepatoma cells. These results indicate that AGCF2 has a differential function between these cells and contributes to the glia-dependent angiotensinogen promoter activity.
Yanai
K
K
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Matsuyama
S
S
Murakami
K
K
Fukamizu
A
A
D000808:Angiotensinogen / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D004247:DNA; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D009687:Nuclear Proteins; D012333:RNA, Messenger; D000808:Angiotensinogen; D004247:DNA