Cloning and expression of a cysteine-rich venom protein from Trimeresurus mucrosquamatus (Taiwan habu).
A full-length cDNA for cysteine-rich venom protein (CRVP) was constructed by immunoscreening and 5'-rapid amplification of cDNA ends from a cDNA library of venom gland of Trimeresurus mucrosquamatus. The predicted CRVP consisted of 183 amino acid residues including a putative signal peptide of 21 residues. Northern blot hybridization suggested the tissue-specific expression in venom gland and its corresponding length of cDNA. The predicted amino acid sequence of CRVP was homologous to a rat epididymal metalloprotein and a lizard helothermine. Amino acid sequence analysis suggested that CRVP may be a venom metalloprotein targeted against ryanodine receptors and Ca2+ release. Moreover, CRVP expressed in Escherichia coli exhibited the same antigenicity as their native venom forms of T. mucrosquamatus. This is the first report in the cloning and expression of a CRVP from the venom gland of T. mucrosquamatus.
Chang
T Y
TY
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Mao
S H
SH
Guo
Y W
YW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003435:Crotalid Venoms / Q000737:chemistry* / Q000302:isolation & purification*; D018076:DNA, Complementary / Q000302:isolation & purification; D004926:Escherichia coli; D005815:Genetic Code; D005822:Genetic Vectors; D008116:Lizards; D008969:Molecular Sequence Data; D009498:Neurotoxins; D011506:Proteins / Q000302:isolation & purification*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D030162:Reptilian Proteins; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
C107708:CRVP protein, Trimeresurus mucrosquamatus; D003435:Crotalid Venoms; D018076:DNA, Complementary; D009498:Neurotoxins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D030162:Reptilian Proteins; C029748:Trimeresurus venoms