[Interaction of ribosomes with membranes in vitro].
Fluorescent "fusion-reporting" probe (R18) was used to study the interaction of ribosomes with membranes in vitro. The latter was incorporated both in the membranes, and ribosomes. The interaction of R18-labeled ribosomes with non-labeled liposomes (of different size and composition) or microsomes increased the fluorescence observed, i.e., the dilution of fluorescent probe took place. The dependence of interaction process on the change of liposomes indicates that the interaction between ribosomes and negatively charged liposomes was more efficient, than that with neutral ones. It is shown that ribosomes can interact with phospholipid membranes even after degradation of protein components accessible for proteins. The interaction of R18-labeled membranes with non-labeled ribosomes results in the increase of fluorescence too. Results obtained indicate to the possibility of direct interaction between ribosomes and membranes.
Borisova
T A
TA
Trikash
I O
IO
Lishko
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VK
D002474:Cell-Free System; D004058:Diffusion; D005456:Fluorescent Dyes; D008081:Liposomes; D008566:Membranes / Q000378:metabolism; D008567:Membranes, Artificial; D008861:Microsomes / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D014908:Triticum
D005456:Fluorescent Dyes; D008081:Liposomes; D008567:Membranes, Artificial
Vzaimodeĭstvie ribosom s membranami in vitro.