Antitumor activity of novel tricyclic pyrone analogs in murine leukemia cells in vitro.
New tricyclic pyrone derivatives were synthesized and tested for their ability to prevent L1210 leukemic cells from synthesizing DNA and growing in vitro. At 50 microM, a pyripyropene analog has no effect, whereas four pentahydro-3-aryl-1-oxopyrano[4,3-b][1]benzopyrans all inhibit DNA synthesis by 79-91% and tumor cell growth by 93-100%. These inhibitory effects are concentration dependent with IC50 around 8.5 microM for DNA synthesis at 2 hours and 1.1 microM for tumor cell growth at 4 days. The aryl groups of these antitumor agents are either 3,4-dimethoxyphenyl or 3-pyridyl. Introduction of a methyl group at C5a and a formyloxy or hydroxy group at C6 does not alter the antitumor effects of the 3,4-dimethoxyphenyl benzopyrans but reduces those of the 3-pyridyl benzopyrans, which, at 50 microM, inhibit DNA synthesis by only 32-49% and fail to alter tumor cell growth. The 4-hydroxy-6-(3-pyridyl)-2-pyrone has no effect and the tricyclic pyrones lacking aryl groups have very little inhibitory effects on DNA synthesis, suggesting that a greater conjugation is required for the antitumor activity. These molecules have never been reported and might be valuable to develop a new class of anticancer drugs.
Perchellet
J P
JP
Department of Chemistry, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.
Newell
S W
SW
Ladesich
J B
JB
Perchellet
E M
EM
Chen
Y
Y
Hua
D H
DH
Kraft
S L
SL
Basaraba
R J
RJ
Omura
S
S
Tomoda
H
H
D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000138:chemical synthesis; D007939:Leukemia L1210 / Q000188:drug therapy*; D011753:Pyrones / Q000138:chemical synthesis / Q000494:pharmacology*
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D006131:Growth Inhibitors; D011753:Pyrones