Optimization of the synthesis of peptide combinatorial libraries using a one-pot method.
Conditions for the synthesis of synthetic peptide combinatorial libraries (SPCLs) from mixtures of amino acids were explored. In a one-pot synthesis, the effect of the starting concentrations of amino acids on the resulting library composition was studied, and the optimum balance of amino acids was determined. Protein sequencing, MALDI-TOF, and amino acid analysis were used for the evaluation of the libraries, and their relative merits-are discussed. The effects of continuous-flow automated synthesis instrumentation in conjunction with polyethylene glycol-polystyrene (PEG-PS) graft supports and various cleavage cocktails on the successful synthesis of SPCLs were examined.
Herman
L W
LW
PerSeptive Biosystems, Framingham, MA 01701, USA.
Tarr
G
G
Kates
S A
SA
D000596:Amino Acids / Q000737:chemistry; D002625:Chemistry, Organic / Q000379:methods; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D019151:Peptide Library; D011092:Polyethylene Glycols; D011137:Polystyrenes; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000596:Amino Acids; D009842:Oligopeptides; D019151:Peptide Library; D011137:Polystyrenes; D011092:Polyethylene Glycols