title: The pyruvate dehydrogenase complex of the chemolithoautotrophic bacterium Thiobacillus ferrooxidans has an unusual E2-E3 subunit fusion.
abstract: The genes encoding pyruvate dehydrogenase (PDH) of Thiobacillus ferrooxidans were previously located by cloning and sequence analysis of the region upstream of the genes encoding the citrate synthase and gamma glutamylcysteine synthetase genes. The pdh genes of T. ferrooxidans were able to complement an Escherichia coli aroP-lpd mutant for growth on minimal medium lacking acetate, indicating that the T. ferrooxidans PDH complex was functional in E. coli. The predicted amino acid sequence of the T. ferrooxidans PDH complex contained three ORFs. The first ORF encoded a 36.7 kDa homologue of the PDH complex E1 alpha subunit, the second ORF a 37.4 kDa E1 beta subunit and the third ORF an unusual 102 kDa fusion of the E2 and E3 subunits. In spite of T. ferrooxidans being a Gram-negative bacterium, its PDH complex had more features in common with Gram-positive bacteria and eukaryotes.
authors.affiliation: Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa.
authors.forename: Rosamund
authors.initials: R
authors.lastname: Powles
authors.affiliation: Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa.
authors.forename: Douglas
authors.initials: D
authors.lastname: Rawlings
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry / Q000235:genetics*; D017421:Sequence Analysis; D013855:Thiobacillus / Q000235:genetics*
chemical_list: D011768:Pyruvate Dehydrogenase Complex