Development of an ELISA for chum salmon (Oncorhynchus keta) growth hormone.
A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of low levels of serum growth hormone (GH) in chum salmon (Oncorhynchus keta). The antiserum to GH (a-rsGH) was obtained from a rabbit immunized with recombinant chum salmon GH. The noncompetitive ELISA was performed by a sandwich method using a-rsGH rabbit IgG as the first antibody, its biotinylated Fab' fragment as the second antibody, and the avidin-biotin reaction for signal amplification. This assay could be run in 3 days and routinely detected GH at concentrations as low as 0.5 ng/ml. The development of an ELISA for GH made possible quantification of serum GH levels. In this assay system, parallel dilution curves were obtained using purified chum salmon GH and GH's from several species of the genus Oncorhynchus.
Fukada
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H
Department of Biology and Aquaculture, Faculty of Fisheries, Hokkaido University, Japan.
Hiramatsu
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N
Gen
K
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Hara
A
A
D000818:Animals; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D013006:Growth Hormone / Q000097:blood*; D007106:Immune Sera; D007074:Immunoglobulin G / Q000276:immunology; D017685:Oncorhynchus keta / Q000097:blood*; D010902:Pituitary Gland / Q000737:chemistry; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012487:Salmonidae; D012965:Sodium Chloride
D007106:Immune Sera; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C061765:salmon growth hormone type II; D012965:Sodium Chloride; D013006:Growth Hormone