Fluorescence of chromatin DNA induced by antitumoral naphthalimides.
Treatment of chicken blood smears and semithin sections from Epon-embedded mouse tissues with aqueous solutions of the 3-aminonaphthalimides FA-142, FA-2043, and FA-2143 induced a strong green-yellow fluorescence of chromatin under violet or violet-blue excitation. Chromatin emission was abolished by previous DNase or hot TCA treatment. The use of 3-methoxy (FA-655) and 3-nitro derivatives (M-4212 and M-12210) resulted in very weak fluorescence of chromatin. Absorption maxima at 346 and 408 nm and an emission peak at 570 nm were observed for the free compound FA-142. Fluorescence properties open new and interesting applications for some of these antitumoral and DNA-intercalating naphthalimides.
Stockert
J C
JC
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Cañete
M
M
Villanueva
A
A
Juarranz
A
A
Trigoso
C I
CI
Braña
M F
MF
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002645:Chickens; D002843:Chromatin / Q000187:drug effects* / Q000648:ultrastructure; D004247:DNA / Q000097:blood / Q000187:drug effects*; D004853:Epoxy Resins; D004912:Erythrocytes; D007094:Imides / Q000494:pharmacology*; D007420:Intestine, Large / Q000166:cytology; D007546:Isoquinolines / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000166:cytology; D051379:Mice; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D002843:Chromatin; D004853:Epoxy Resins; D007094:Imides; D007546:Isoquinolines; C004875:EPON; D004247:DNA