Studies on the effect of ethanol on the Na+, and the Na+, K(+)-ATPase activities of plasma membranes of rat kidney proximal tubular cells.
In the present work it was investigated the effect of 2% ethanol on the Na+ and on the Na+, K(+)-ATPase activities. The differential effect of the alcohol on the two ATPases (approximately 40% inhibition of the Na(+)-ATPase and approximately 10% inhibition of the Na+, K(+)-ATPase), is not due to a higher degree of denaturalization of the enzyme, nor to a faster effect of ethanol on the Na(+)-than on the Na+, K(+)-ATPase. Our results show that ethanol affects the selectivity of the Na+, K(+)-ATPase for Na+ and/or for K+, enhancing the Na+ affinity for the K+ sites, and/or reducing the K+ affinity for its own sites. This effect was not seen for the Na(+)-ATPase, indicating that 2% ethanol inhibits the two ATPases in a totally different way.
Rothman
A
A
Laboratorio de Bioenergética Celular, Instituto Venezolano de Investigaciones Cientifícas (IVIC), Caracas, Venezuela.
Proverbio
T
T
Proverbio
F
F
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology*; D000431:Ethanol / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000648:ultrastructure*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000378:metabolism*
D000431:Ethanol; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase