title: Virus isolation from wild-caught mosquitoes during a Japanese encephalitis outbreak in Kerala in 1996.
abstract: Out of 5357 wild-caught mosquitoes in 163 pools tested for virus using antigen capture ELISA and an insect-bioassay (inoculation into Toxorhynchites splendens larvae and identification by IFA using JE virus-specific monoclonal antibody), 16 flavivirus isolations were made of which 12 (75%) were identified as JE virus. Of the 12 JE virus isolations, 7 were from Culex tritaeniorhynchus, 3 from Mansonia uniformis and 1 each from Ma. indiana and Anopheles subpictus. Four isolations from Mansonia species for the first time reported here are noteworthy.
authors.affiliation: Vector Control Research Centre, Pondicherry.
authors.forename: V
authors.initials: V
authors.lastname: Dhanda
authors.forename: V
authors.initials: V
authors.lastname: Thenmozhi
authors.forename: N P
authors.initials: NP
authors.lastname: Kumar
authors.forename: J
authors.initials: J
authors.lastname: Hiriyan
authors.forename: N
authors.initials: N
authors.lastname: Arunachalam
authors.forename: A
authors.initials: A
authors.lastname: Balasubramanian
authors.forename: A
authors.initials: A
authors.lastname: Ilango
authors.forename: A
authors.initials: A
authors.lastname: Gajanana
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D009033:Culicidae / Q000821:virology*; D004196:Disease Outbreaks; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000453:epidemiology*; D004797:Enzyme-Linked Immunosorbent Assay; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007194:India / Q000453:epidemiology
chemical_list: D000911:Antibodies, Monoclonal