Catalase activity in erythrocytes from colon and gastric cancer patients. Influence of nickel, lead, mercury, and cadmium.
Catalase (CAT) is an enzyme that is involved in antioxidant defense, cell growth, and is possibly associated with tumoral processes. In this paper, the results of experiments designed to determine the influence of metallic carcinogens such as nickel (Ni), lead (Pb), mercury (Hg), and cadmium (Cd), on CAT activity are reported. CAT activity was measured in erythrocytes from three groups: a group of colon cancer patients, a group of gastric cancer patients before clinical treatment, and a control group of healthy blood donors. Concentrations of this enzyme are significantly higher than controls in the colon cancer group, but lower in gastric neoplasia. By generating highly reactive oxygenated species, Ni, Pb, Hg, and Cd alter catalase activity. Solutions of Ni, Cd, and Pb at 0.2 mM concentrations inhibit CAT activity in colon cancer, but increase it in gastric neoplasia. Hg activates CAT in colon cancer, and causes a slightly increased activity in gastric cancer. No complete deactivation of the enzyme was observed.
Martín Mateo
M C
MC
Biochemistry Department, Faculty of Sciences, Universidad de Valladolid, Spain.
Martín
B
B
Santos Beneit
M
M
Rabadán
J
J
D002104:Cadmium / Q000009:adverse effects; D002273:Carcinogens / Q000009:adverse effects*; D002374:Catalase / Q000097:blood* / Q000187:drug effects; D003110:Colonic Neoplasms / Q000097:blood / Q000201:enzymology* / Q000503:physiopathology; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007854:Lead / Q000009:adverse effects; D008628:Mercury / Q000009:adverse effects; D009532:Nickel / Q000009:adverse effects; D017382:Reactive Oxygen Species; D013056:Spectrophotometry, Ultraviolet; D013274:Stomach Neoplasms / Q000097:blood / Q000201:enzymology* / Q000503:physiopathology
D002273:Carcinogens; D006454:Hemoglobins; D017382:Reactive Oxygen Species; D002104:Cadmium; D007854:Lead; D009532:Nickel; D002374:Catalase; D008628:Mercury