Subtractive cloning: past, present, and future.
Subtractive cloning is a powerful technique for isolating genes expressed or present in one cell population but not in another. This method and a related one termed positive selection have their origins in nucleic acid reassociation techniques. We discuss the history of subtractive techniques, and fundamental information about the nucleic acid composition of cells that came out of reassociation analyses. We then explore current techniques for subtractive cloning and positive selection, discussing the merits of each. These techniques include cDNA library-based techniques and PCR-based techniques. Finally, we briefly discuss the future of subtractive cloning and new approaches that may augment or supersede current methods.
Sagerström
C G
CG
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
Sun
B I
BI
Sive
H L
HL
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D005544:Forecasting; D006801:Humans