Oxygenation capacity of hypotonized and crosslinked rat erythrocytes.
Rat erythrocytes subjected to hypotonic-isotonic dialysis, or crosslinking with bifunctional reagents (glutaraldehyde and dimethyl suberimidate hydrochloride) show a high percentage of methemoglobin and decreased oxyhemoglobin content which implies a low oxygen carrying capacity. Such modified cells maintain reversible oxygen binding properties although, they present a high hemoglobin oxygen affinity (low P50) and a diminished cooperativity in binding oxygen to hemoglobin (low n). These results suggest a reduced capacity of liberating oxygen to tissues under low PO2. Changes produced in erythrocytes can not be restored even in the presence of energy (ATP), reduced glutathione and 2,3-bisphosphoglyceric acid during the dialysis process or after crosslinking/permeabilizing treatment.
García-Pérez
A I
AI
Departamento de Bioquímica y Biología Molecular. Universidad de Alcalá. Campus Universitario, Madrid, Spain.
Pérez
M T
MT
Lucas
L
L
Pinilla
M
M
Luque
J
J
Sancho
P
P
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D003432:Cross-Linking Reagents; D003956:Dialysis; D004120:Dimethyl Suberimidate; D004163:Diphosphoglyceric Acids / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism*; D005976:Glutaral; D005978:Glutathione / Q000494:pharmacology; D006447:Hemoglobin H / Q000378:metabolism; D007038:Hypotonic Solutions; D008297:Male; D008706:Methemoglobin / Q000378:metabolism; D009996:Osmotic Fragility; D010100:Oxygen / Q000097:blood*; D010108:Oxyhemoglobins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D003432:Cross-Linking Reagents; D004163:Diphosphoglyceric Acids; D007038:Hypotonic Solutions; D010108:Oxyhemoglobins; D019794:2,3-Diphosphoglycerate; D004120:Dimethyl Suberimidate; D000255:Adenosine Triphosphate; D008706:Methemoglobin; D006447:Hemoglobin H; D005978:Glutathione; D010100:Oxygen; D005976:Glutaral