title: Effects of combined topical metronidazole and mechanical treatment on the subgingival flora in deep periodontal pockets in cuspids and bicuspids.
abstract: The Effect on the subgingival microflora of a single topical administration of a 95% collagen and 5% metronidazole device in combination with debridement was investigated in 30 adult periodontitis patients in comparison with mechanical treatment alone. For each patient, plaque samples from test and control sites in cuspids and bicuspids were collected for culture and enumeration of total anaerobically cultivable bacteria (TA), black-pigmented anaerobes (BPA), and Actinobacillus actinomycetemcomitans (Aa). Spirochetes and fusiforms were quantified by direct microscopic examination after Giemsa staining. A decrease was observed for all parameters, and a significant difference in comparison with the control group was found for fusiforms. After treatment, a lower number of Aa positive sites were observed in the test group (13/25). These results show that a single application of topical metronidazole seems to be effective as adjunctive antimicrobial treatment in adult periodontitis.
authors.affiliation: Department of Periodontology, Dental School, University of Nice-Sophia, Antipolis, France.
authors.forename: C
authors.initials: C
authors.lastname: Hitzig
authors.forename: T
authors.initials: T
authors.lastname: Fosse
authors.forename: Y
authors.initials: Y
authors.lastname: Charbit
authors.forename: C
authors.initials: C
authors.lastname: Bitton
authors.forename: L
authors.initials: L
authors.lastname: Hannoun
mesh_terms: D000284:Administration, Oral; D000287:Administration, Topical; D000328:Adult; D000368:Aged; D016976:Aggregatibacter actinomycetemcomitans / Q000254:growth & development; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000627:therapeutic use*; D001399:Azure Stains; D001419:Bacteria / Q000254:growth & development*; D001421:Bacteria, Anaerobic / Q000254:growth & development; D001641:Bicuspid; D003094:Collagen / Q000627:therapeutic use; D004396:Coloring Agents; D003131:Combined Modality Therapy; D003481:Cuspid; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000008:administration & dosage / Q000627:therapeutic use*; D008875:Middle Aged; D010514:Periodontal Pocket / Q000188:drug therapy / Q000382:microbiology* / Q000628:therapy; D010518:Periodontitis / Q000188:drug therapy / Q000382:microbiology / Q000628:therapy; D017861:Porphyromonas / Q000254:growth & development; D018720:Prevotella / Q000254:growth & development; D013144:Spirochaetales / Q000254:growth & development; D013357:Subgingival Curettage
chemical_list: D000891:Anti-Infective Agents, Local; D001399:Azure Stains; D004396:Coloring Agents; D008795:Metronidazole; D003094:Collagen