Medium-reductant directed expression of methyl coenzyme M reductase isoenzymes in Methanobacterium thermoautotrophicum (strain deltaH).
Methanobacterium thermoautotrophicum was grown in a chemostat under various controlled conditions in the presence of either sodium sulfide or sodium thiosulfate. After establishment of the steady state, cells were taken and examined for expression of the mRNA transcripts coding for the different forms of methyl coenzyme M reductase (MCR) and methylene tetrahydomethanopterin dehydrogenase (MDH). MCR isoenzyme II expression varied most markedly. Expression was found not only to depend on known parameters temperature, pH and gassing rate, but also on the medium composition, especially the reductant present.
Pennings
J L
JL
Department of Microbiology, University of Nijmegen, The Netherlands. pennings@sci.kun.nl
de Wijs
J L
JL
Keltjens
J T
JT
van der Drift
C
C
D001483:Base Sequence; D003470:Culture Media / Q000494:pharmacology; D017931:DNA Primers; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D017014:Methanobacterium / Q000187:drug effects / Q000201:enzymology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000096:biosynthesis* / Q000235:genetics; D019163:Reducing Agents / Q000494:pharmacology; D013440:Sulfides / Q000494:pharmacology; D013885:Thiosulfates / Q000494:pharmacology
D003470:Culture Media; D017931:DNA Primers; D007527:Isoenzymes; D019163:Reducing Agents; D013440:Sulfides; D013885:Thiosulfates; D010088:Oxidoreductases; D000587:Oxidoreductases Acting on CH-NH Group Donors; C047126:methylenetetrahydromethanopterin dehydrogenase; C021572:methyl coenzyme M reductase; C017717:sodium thiosulfate; C033479:sodium sulfide