The avian chB6 (Bu-1) alloantigen can mediate rapid cell death.
The control of cell death is critical in the immune system. T and B lymphocytes must be censored during their development to remove nonfunctional or self-reactive lymphocytes. However, the molecules controlling cell deletion during lymphopoiesis have not been defined. B cells removed from the avian bursa of Fabricius rapidly undergo cell death in culture. We screened bursal B cells with a panel of Abs and lectins to identify molecules affecting their viability. Abs to the chB6 alloantigen caused a rapid loss of cell viability as measured by staining with propidium iodide. ChB6 Abs also cause adhesion between B cells. Transfection of cDNA encoding chB6 reconstituted the allele-specific cell death and adhesion effects in avian cell lines. These effects can be separated by binding cells onto Ab-coated plastic dishes. In these experiments, cells were killed in the absence of cell:cell contact. The ability of chB6 cross-linking to evoke cell aggregation and cell death is also observed when chB6 is expressed in growth factor-dependent mammalian cells. In these cells growth factor can almost completely prevent cell death but not cell aggregation. This suggests that known cell survival stimuli can suppress the cell death brought about by chB6 cross-linking. These results show that chB6 may have an important role in controlling cell survival and/or adhesion during avian B cell development.
Funk
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PE
Howard Hughes Medical Institute, Gwen Knapp Center for Lupus and Immunology Research, The University of Chicago, IL 60637, USA.
Tregaskes
C A
CA
Young
J R
JR
Thompson
C B
CB
D000818:Animals; D017209:Apoptosis; D001402:B-Lymphocytes / Q000502:physiology*; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D007377:Interleukin-3 / Q000502:physiology; D007519:Isoantigens / Q000502:physiology*; D008969:Molecular Sequence Data; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007377:Interleukin-3; D007519:Isoantigens; D013755:Tetradecanoylphorbol Acetate