title: Amperometric bienzymic sensor for aspartame.
abstract: An amperometric enzyme electrode for the determination of aspartame was developed by covalent immobilization of alcohol oxidase and alpha-chymotrypsin. A platinum based hydrogen peroxide electrode was used as the detector. Excellent sensitivity was obtained using batch, flow-through and flow injection methods with detection limits of 2 x 10(-7), 4 x 10(-7) and 10(-6) mol l-1, respectively. Different strategies for eliminating interfering compounds, including the introduction of an additional alcohol oxidase-catalase membrane and signal subtraction using an alcohol electrode, were employed. A recovery study on seven food samples was carried out and the results were satisfactory.
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Compagnone
authors.forename: D
authors.initials: D
authors.lastname: O'Sullivan
authors.forename: G G
authors.initials: GG
authors.lastname: Guilbault
mesh_terms: D001218:Aspartame / Q000032:analysis*; D015374:Biosensing Techniques; D005504:Food Analysis / Q000379:methods; D013549:Sweetening Agents / Q000032:analysis*
chemical_list: D013549:Sweetening Agents; D001218:Aspartame