Photochemical purging of autologous bone marrow grafts: assessment of damage to stem cells and the microenvironment in long-term marrow cultures.
Toxicity of merocyanine 540 (MC540) was assessed in long-term (Dexter-type) bone marrow cultures and in a short-term in vitro clonal assay of fibroblast colony-forming cells (CFU-F). Exposure of freshly explanted mouse bone marrow cells to MC540 (15 micrograms/ml) and white light (fluence: 126 kJ/m2) reduced CFU-F by approximately 2 logs but did not abrogate the cells' capacity to establish and maintain long-term bone marrow cultures. Fat cells were rare or absence in adherent layers established with photosensitized bone marrow cells but the cultures' capacity to generate non-adherent cells, granulocyte/macrophage progenitors (CFU-GM), and early erythroid progenitors (BFU-E) was only moderately (28-36%) reduced.
Kubo
Y
Y
Department of Pediatrics, Medical College of Wisconsin, Milwaukee 53226, USA.
Sieber
F
F
D000818:Animals; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000473:pathology; D051379:Mice; D017319:Photosensitizing Agents; D011744:Pyrimidinones; D017154:Stromal Cells / Q000473:pathology; D014182:Transplantation, Autologous
D017319:Photosensitizing Agents; D011744:Pyrimidinones; C003954:merocyanine dye