Determination of sugar specificity of jackfruit lectin by a simple sugar-lectin binding assay using microtiter plate.
Sugar-lectin binding assay was developed as a simple method which employed direct coating of microtiter plate with galactose-binding lectins. Biotin-galactose conjugate was used to bind to the immobilized lectins. The bound conjugate was then detected using streptavidin-horseradish peroxidase. Using the assay in conjunction with various competing carbohydrates, jackfruit lectin from Artocarpus heterophyllus was found to be specific for alpha-anomer of galactoside with an aromatic residue.
Wetprasit
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N
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Chulavatnatol
M
M
D000818:Animals; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D001710:Biotin / Q000494:pharmacology; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D006384:Hemagglutination / Q000187:drug effects; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D037102:Lectins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D012680:Sensitivity and Specificity; D019809:Streptavidin; D013379:Substrate Specificity
D001426:Bacterial Proteins; D002241:Carbohydrates; D037102:Lectins; D037121:Plant Lectins; C058865:Strep-avidin conjugated horseradish peroxidase; D001710:Biotin; D019809:Streptavidin; D006735:Horseradish Peroxidase