title: A Bacillus subtilis gene encoding a protein similar to nucleotide sugar transferases influences cell shape and viability.
abstract: Bacillus subtilis gene ypfP, which is located at 196 degrees on the genetic map, shows similarity to both the monogalactosyldiacylglycerol synthase gene of Cucumis sativus, which encodes a galactosyltransferase, and the murG genes of B. subtilis, Escherichia coli, Haemophilus influenzae, and Synechocystis sp. strain PCC6803, which encode N-acetylglucosaminyltransferases involved in peptidoglycan biosynthesis. Cells containing a null mutation of ypfP are shorter and rounder than wild-type cells during growth in Luria-Bertani medium and glucose minimal medium. In addition, the mutant cells preferentially undergo lysis when grown on solid Luria-Bertani medium.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Price
authors.forename: S
authors.initials: S
authors.lastname: Roels
authors.forename: R
authors.initials: R
authors.lastname: Losick
mesh_terms: D000217:Acyltransferases / Q000235:genetics; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D016695:Glycosyltransferases; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics; D009711:Nucleotides / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009711:Nucleotides; D000217:Acyltransferases; C058055:galactosylacylglycerol O-acyltransferase; D016695:Glycosyltransferases; C108438:YpfP protein, Bacillus subtilis; D017351:N-Acetylglucosaminyltransferases; C064057:UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase