title: Separation of disaccharides as their borate complexes by capillary electrophoresis with indirect detection in visible range.
abstract: Borate complexation was used to make possible the separation of disaccharides by capillary electrophoresis with indirect detection. A high borate concentration did not affect the indirect detection sensitivity in as negative a way as predicted previously. The concentration sensitivity for sucrose was determined to be 2 mM at the borate concentration of 200 mM in running electrolyte. The newly introduced background [corrected] chromophore, p-nitrophenol, allows the monitoring of the separation process in a visible range at 400 nm. This also enables the indirect detection of UV-absorbing compounds in complex mixtures in which they would be impossible to detect with a UV-absorbing background [corrected] chromophore.
authors.affiliation: Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic. plocek@ipm.cz
authors.forename: J
authors.initials: J
authors.lastname: Plocek
authors.forename: J
authors.initials: J
authors.lastname: Chmelik
mesh_terms: D001881:Borates / Q000737:chemistry / Q000302:isolation & purification*; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D009596:Nitrophenols / Q000302:isolation & purification
chemical_list: D001881:Borates; D004187:Disaccharides; D009596:Nitrophenols; C024836:4-nitrophenol