title: Secretion of apolipoprotein A-I by calf liver parenchymal cells.
abstract: OBJECTIVE
  To determine: whether apolipoprotein A-I (apoA-I) is secreted by calf liver parenchymal cells; the isoprotein pattern and association with lipids of secreted apoA-I; and effects of steroid hormones on apoA-I secretion.
  
  
  METHODS
  6 male Holstein calves (1 to 2 weeks old) as a cell culture source.
  
  
  METHODS
  apoA-I in culture medium was detected by immunoblot analysis, and its concentration was measured by use of an ELISA. The isoprotein pattern was analyzed by use of two-dimensional polyacrylamide gel electrophoresis. Associations of apoA-I with cholesterol and phospholipids were examined by ultracentrifugal analysis of the culture medium.
  
  
  RESULTS
  Concentration of apoA-I in culture medium increased linearly up to 24 hours. The protein synthesis inhibitors, actinomycin D and cycloheximide, suppressed apoA-I accumulation in the medium in dose-dependent manner. Molecular mass of this protein in culture medium was 28 kd, and was indistinguishable from apoA-I of plasma. Four isoproteins with different isoelectric points (1 = 5.75; 2 = 5.67; 3 = 5.58; and 4 = 5.46) were detected. Of these, isoprotein 2 was the major species. By comparison, isoprotein 4 was the predominant species in plasma, and isoprotein 5 (isoelectric point = 5.38) was newly detected instead of isoprotein 1. Approximately 33% of apoA-I in culture medium was found in lipid-rich fractions, whereas the rest was found in nonlipoprotein fractions. Dexamethasone (10(-8) to 10(-6) M) significantly (P < 0.001) increased apoA-I concentration in the medium, and the stimulatory effect was significantly (P < 0.001) suppressed by the simultaneous addition of 10(-6) M progesterone. Progesterone itself (10(-8) to 10(-5) M) had little effect on apoA-I secretion; estradiol (10(-14) to 10(-8) M) also had no significant effect.
  
  
  CONCLUSIONS
  apoA-I is synthesized by calf liver parenchymal cells, and the secreted protein is modified during circulation. Moreover, apoA-I synthesis and secretion by the cells appear not to be largely influenced by hormones, except for dexamethasone.
authors.affiliation: Laboratory of Biochemistry, National Institute of Animal Health, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
authors.forename: N
authors.initials: N
authors.lastname: Katoh
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D011374:Progesterone / Q000494:pharmacology
chemical_list: D016632:Apolipoprotein A-I; D003609:Dactinomycin; D011374:Progesterone; D003907:Dexamethasone; D003513:Cycloheximide