title: Identification of sn-2 acetyl glycerophosphocholines in human keratinocytes.
abstract: Evidence is accumulating suggesting that platelet-activating factor plays a role in inflammatory dermatoses. Mass spectrometric methods were used to examine the molecular species of sn-2 acetyl glycerophosphocholines (GPC) synthesized by primary cultures of human neonatal foreskin-derived keratinocytes. Ionophore-stimulated keratinocytes synthesize both 1-alkyl and 1-acyl sn-2 acetyl-GPC, and the relative amounts were as follows: hexadecyl > palmitoyl > octadecyl > stearoyl at the sn-1 position. PAF synthesis in the keratinocyte-derived cell line HaCaT was inhibited by dexamethasone, suggesting that the anti-inflammatory effects of glucocorticosteroids in inflammatory dermatoses might be in part related to the inhibition of the synthesis of mediators such as PAF.
authors.affiliation: Department of Dermatology, Indiana University School of Medicine, Indianapolis, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Travers
authors.forename: C
authors.initials: C
authors.lastname: Johnson
authors.forename: K L
authors.initials: KL
authors.lastname: Clay
authors.forename: K
authors.initials: K
authors.lastname: Harrison
authors.forename: T
authors.initials: T
authors.lastname: Zekman
authors.forename: J G
authors.initials: JG
authors.lastname: Morelli
authors.forename: R C
authors.initials: RC
authors.lastname: Murphy
mesh_terms: D000305:Adrenal Cortex Hormones / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D016718:Arachidonic Acid / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D007476:Ionophores / Q000494:pharmacology; D015603:Keratinocytes / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000032:analysis / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
chemical_list: C069030:1-acyl-2-acetyl-sn-glycero-3-phosphocholine; D000305:Adrenal Cortex Hormones; D000893:Anti-Inflammatory Agents; D007476:Ionophores; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D000001:Calcimycin; D003907:Dexamethasone