RNA polymerase I from S. cerevisiae depends on an additional factor to release terminated transcripts from the template.
Terminated transcripts were generated at the ends of linearized DNA templates and at DNA-bound lac repressor by in vitro transcription with highly enriched or purified yeast RNA polymerase I (pol I). The release of the synthesized transcripts from the DNA was analyzed using immobilized DNA as template for the transcription reaction. An additional activity distinguishable from pol I was necessary to remove the terminated RNA from the template. Efficiency of transcript release could be improved if a thymidine-rich DNA fragment was located upstream of the transcriptional arrest caused by the DNA-bound lac repressor. The release activity interacted with different forms of polymerases, pol I able to initiate on the ribosomal gene promoter and pol I only active in non-specific transcription.
Tschochne
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H
BZH, Biochemie-Zentrum Heidelberg, Germany. IM4@ix.urz.uni-heidelberg.de
Milkereit
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D001665:Binding Sites; D004271:DNA, Fungal / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger; D012318:RNA Polymerase I