Expression of transforming growth factor beta in radiation interstitial pneumonitis.
We investigated the changes in transforming growth factor beta 1 (TGF-beta 1) mRNA and TGF-beta 3 protein expression that occur in radiation interstitial pneumonitis. We used TGF-beta 1-cDNA probe in situ hybridization and TGF-beta 3 polyclonal antibody in immunohistochemical techniques. Our results showed that the distribution of TGF-beta 1 mRNA and TGF-beta 3 protein basically coincided in blood vessels, airways, lung parenchyma, and alveolar macrophages. However, bronchial epithelial cells expressed only TGF-beta 3 proteins and no TGF-beta 1 mRNA. We found an increased expression of TGF-beta 1 mRNA and TGF-beta 3 proteins in radiation interstitial pneumonitis.
Bai
Y H
YH
Department of Experimental Pathology, Institute of Radiation Medicine, Beijing, P.R. China.
Wang
D W
DW
Cui
X M
XM
Long
J Y
JY
Cui
C B
CB
Jia
C Y
CY
Song
L W
LW
D000818:Animals; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D011833:Radiation Injuries, Experimental / Q000378:metabolism* / Q000473:pathology; D017564:Radiation Pneumonitis / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta