Mechanism of cryoprecipitation of anti-blood group B murine monoclonal antibodies.
In our experience, some examples of mouse monoclonal antibodies of anti-B blood group specificity develop a precipitate when stored at 4 degrees C. This poses problems during the preparation of blood grouping reagents containing anti-B, and in the storage and use of such reagents. Here we show that this problem can be circumvented by alteration of the glycan moiety of the secreted immunoglobulin, either by glycosidase treatment of the partially purified immunoglobulin, or by the addition of glycan processing inhibitors to the hybridoma cell cultures. These findings have importance for the manufacture of monoclonal antibodies, and highlight a possible new role for carbohydrate in immunoglobulin interaction and immune complex formation.
Judson
P A
PA
South Western Regional Blood Transfusion Centre, Bristol, U.K.
Smythe
J S
JS
D017485:1-Deoxynojirimycin / Q000494:pharmacology; D000017:ABO Blood-Group System / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D002240:Carbohydrate Sequence; D002460:Cell Line; D011232:Chemical Precipitation; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005959:Glucosidases / Q000037:antagonists & inhibitors; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism; D007075:Immunoglobulin M / Q000737:chemistry / Q000276:immunology*; D007212:Indolizines / Q000494:pharmacology; D008361:Mannosidases / Q000037:antagonists & inhibitors; D051379:Mice; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology; D014407:Tumor Cells, Cultured; D000519:alpha-Galactosidase / Q000494:pharmacology; D043323:alpha-Mannosidase; D054836:Indolizidines
D017485:1-Deoxynojirimycin; D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D004791:Enzyme Inhibitors; D005959:Glucosidases; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007212:Indolizines; D008361:Mannosidases; D011134:Polysaccharides; D000519:alpha-Galactosidase; D043323:alpha-Mannosidase; C037806:castanospermine; D054836:Indolizidines