Effects of apo E deficiency on plasma lipid levels in mice lacking APOBEC-1.
Apolipoprotein (apo) B100 mRNA undergoes site specific C to U editing, generating a stop-translation codon of apo B48 in the small intestine. This reaction is catalyzed in an editosome which contains APOBEC-1, a catalytic subunit. To clarify the functional significance of the apo B mRNA editing in lipoprotein metabolism, we have generated APOBEC-1 knockout mice and double knockout mice which are deficient in both APOBEC-1 and apo E. The apo B mRNA editing activity was markedly reduced and complete elimination of apo B48 from the plasma was observed in APOBEC-1(-/-) mice. Plasma triglyceride levels significantly increased in the double knockout mice (APOBEC-1(-/-);apo E(-/-)) as compared to apo E(-/-) mice. These results suggest that APOBEC-1(-/-) mice are a valuable model for experiments designed to understand a role of apo B mRNA editing.
Osuga
J
J
The Third Department of Internal Medicine, University of Tokyo, Bunkyo-ku, Japan.
Yagyu
H
H
Ohashi
K
K
Harada
K
K
Yazaki
Y
Y
Yamada
N
N
Ishibashi
S
S
D000071479:APOBEC-1 Deaminase; D000818:Animals; D053299:Apolipoprotein B-100; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000235:genetics* / Q000378:metabolism; D001057:Apolipoproteins E / Q000172:deficiency*; D002784:Cholesterol / Q000097:blood; D003564:Cytidine Deaminase / Q000172:deficiency*; D008055:Lipids / Q000097:blood*; D051379:Mice; D018345:Mice, Knockout; D017393:RNA Editing; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D053299:Apolipoprotein B-100; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D008055:Lipids; D002784:Cholesterol; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D000071479:APOBEC-1 Deaminase; C000605090:Apobec1 protein, mouse; D003564:Cytidine Deaminase