title: Orbital bleeding in rats while under diethylether anaesthesia does not influence telemetrically determined heart rate, body temperature, locomotor and eating activity when compared with anaesthesia alone.
abstract: The question addressed was whether orbital bleeding in rats, while under diethylether anaesthesia, affects their locomotor activity body core temperature, heart rate rhythm and eating pattern. Roman High Avoidance (RHA) and Roman Low Avoidance (RLA) rats were used to enhance generalization of the results. Orbital bleeding when the rats were under diethylether anaesthesia was compared with diethylether anaesthesia alone. To take into account any effects of handling, the rats were also subjected to sham anaesthesia. The RHA rats urinated more during anaesthesia, needed more time to recover from the anaesthesia and showed a greater endocrine stress response to diethylether anaesthesia when compared with the RLA rats. During anaesthesia, the RHA rats showed a greater fall of body temperature and bradycardia than did the RLA rats. Diethylether anaesthesia reduced locomotor activity in the RHA rats, but had no effect in the RLA rats. In neither RHA nor RLA rats did anaesthesia plus orbital puncture, versus anaesthesia alone, influence body temperature, heart rate rhythm, locomotor and eating activity. The lack of effect of orbital puncture occurred both in the short term (within 2h) and long term (within 48 hours) and thus this study indicates that orbital puncture had, at least with respect to variables measured in the present study, no effect superimposed on that of diethylether anaesthesia.
authors.affiliation: Central Laboratory Animal Institute, Utrecht University, The Netherlands. HvH@gdl.ruu.nl
authors.forename: H
authors.initials: H
authors.lastname: Van Herck
authors.forename: S F
authors.initials: SF
authors.lastname: De Boer
authors.forename: A P
authors.initials: AP
authors.lastname: Hesp
authors.forename: H A
authors.initials: HA
authors.lastname: Van Lith
authors.forename: V
authors.initials: V
authors.lastname: Baumans
authors.forename: A C
authors.initials: AC
authors.lastname: Beynen
mesh_terms: D000758:Anesthesia / Q000009:adverse effects / Q000662:veterinary*; D018685:Anesthetics, Inhalation / Q000009:adverse effects*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001800:Blood Specimen Collection / Q000009:adverse effects / Q000379:methods / Q000662:veterinary*; D001831:Body Temperature / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D004435:Eating / Q000502:physiology; D004837:Epinephrine / Q000097:blood; D004986:Ether / Q000009:adverse effects*; D006339:Heart Rate / Q000502:physiology; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009638:Norepinephrine / Q000097:blood; D009915:Orbit; D051381:Rats; D013686:Telemetry / Q000662:veterinary
chemical_list: D018685:Anesthetics, Inhalation; D001786:Blood Glucose; D004986:Ether; D003345:Corticosterone; D009638:Norepinephrine; D004837:Epinephrine