title: The effects of neurotransmitter antagonists and glucose on luteinizing hormone secretion in growth-restricted wethers.
abstract: Chronic undernutrition results in reduced secretion of luteinizing hormone (LH). Two experiments were conducted in wethers whose LH secretion was suppressed by growth restriction caused by feeding a maintenance ration. The first study examined neurotransmitters that may be actively inhibiting LH secretion during growth restriction. The effects of various neurotransmitter antagonists were investigated: pimozide (PIM, dopamine), cyproheptadine (CYP, serotonin), pyrilamine (PYR, H1), cimetidine (CMT, H2) and propranolol (PRO, beta-adrenergic) in wethers specifically fed to maintain a body weight of 28 kg (GR, n = 7) and in full fed control wethers (n = 5). Blood samples were taken at 15 min intervals for 4 h before and after drug administration. Serum LH concentrations were determined by radioimmunoassay (RIA). Only PIM increased (P < 0.01) serum LH pulse frequency in the GR wethers (pre 0.5 +/- 0.2 pulses per 4 h vs. post 2.6 +/- 0.9 pulses per 4 h). None of the drugs tested had an effect on the control wethers. Experiment 2 examined the effect of glucose administration (50, 100, or 150 mg i.v.) on LH secretion in GR wethers. Only the 150 mg dose significantly (P < 0.05) increased LH pulse frequency compared to the pre-injection period (1.1 +/- 0.3 vs. 2.0 +/- 0.4 pulses per 4 h). After refeeding, LH pulse frequency and serum glucose concentrations increased. Proglumide, a cholecystokinin (CCK) antagonist, did not block this increase (2.1 +/- 0.4 vs. 2.2 +/- 0.3 pulses per 4 h). These data suggest that dopamine inhibits LH secretion in GR wethers and that increasing serum glucose concentrations increased LH secretion.
authors.affiliation: Department of Animal Science and Food Technology, Texas Tech University, Lubbock 79409-2141, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Branum
authors.forename: R S
authors.initials: RS
authors.lastname: Kline
authors.forename: C S
authors.initials: CS
authors.lastname: Whisnant
mesh_terms: D000818:Animals; D002927:Cimetidine / Q000494:pharmacology; D003533:Cyproheptadine / Q000494:pharmacology; D005508:Food Deprivation / Q000502:physiology; D005947:Glucose / Q000494:pharmacology* / Q000502:physiology; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D018377:Neurotransmitter Agents / Q000037:antagonists & inhibitors* / Q000502:physiology; D010868:Pimozide / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011738:Pyrilamine / Q000494:pharmacology; D012756:Sheep / Q000254:growth & development / Q000502:physiology*
chemical_list: D018377:Neurotransmitter Agents; D010868:Pimozide; D003533:Cyproheptadine; D002927:Cimetidine; D007986:Luteinizing Hormone; D011433:Propranolol; D011738:Pyrilamine; D005947:Glucose