Characterization of the influence of some cyclodextrins on the stratum corneum from the hairless mouse.
Differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR) spectroscopy and transmission electron microscopy (TEM) have been used to determine the influence of beta-cyclodextrin (beta-CyD), hydroxypropyl-beta-CyD (HP-beta-CyD) and gamma-CyD on the structural properties of the stratum corneum from the hairless mouse. Some modest changes in the stratum corneum lipid transition temperature were induced by HP-beta-CyD and blue shifts were observed in the FTIR spectra of the C-H asymmetric and symmetric stretching of the lipids from the stratum corneum. Results from TEM studies indicated that HP-beta-CyD caused removal and possible disorganization of the lipid matrix that envelopes the corneocytes of the stratum corneum, whereas no effect was seen after treatment of the samples with beta-CyD and gamma-CyD. These results suggest that HP-beta-CyD can increase the permeability of the stratum corneum possibly as a result of extraction of lipids, and might thus enhance drug permeation through the skin.
Bentley
M V
MV
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brasil.
Vianna
R F
RF
Wilson
S
S
Collett
J H
JH
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000818:Animals; D002152:Calorimetry, Differential Scanning; D003505:Cyclodextrins / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008854:Microscopy, Electron; D010539:Permeability / Q000187:drug effects; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; C479659:hydroxypropyl-gamma-cyclodextrin; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C031215:betadex; C023792:gamma-cyclodextrin