Liver and serum antioxidant status after methanol intoxication in rats.
Activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and glutathione reductase (GSSG-R) and concentration of ascorbate, alpha-tocopherol, non-protein and protein-bound sulfhydryl compounds and thiobarbituric acid-reactive substances (TBA-rs) were measured in liver and serum of rats 6, 12 and 24 h and 2, 5 and 7 days after intoxication with 1.5 g or 3.0 g methanol/kg b.w. Liver GSH-Px and GSSG-R activities and SH-groups and ascorbate content were significantly diminished at 6 and 24 h, while TBA-rs were increased. Serum SOD, GSH-Px and GSSG-R activities and SH-groups concentration were reduced while TBA-rs were elevated. The changes were more intensive after application of the higher dose of methanol. It is concluded that methanol impairs the liver and blood serum antioxidant mechanisms in rats.
Skrzydlewska
E
E
Department of Instrumental Analysis, Medical Academy, Białystok, Poland.
Farbiszewski
R
R
D000435:Alcoholic Intoxication / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D000432:Methanol / Q000506:poisoning*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D000432:Methanol