Detection of N7-(2-hydroxyethyl)guanine adducts in DNA and 9L cells treated with 1-(2-chloroethyl)-1-nitrosourea.
A sensitive analytical method, HPLC-ED, was developed for the measurement of N7-(2-hydroxyethyl)guanine (N7-HOEtG). A detection limit of 3.2 N7-HOEtG/10(8) nucleotides was obtained with this method. Linear dose response curves for the formation of N7-HOEtG were obtained following treatment of either calf thymus DNA or 9L cells with 1-(2-chloroethyl)-1-nitrosourea (CNU). Using HPLC-ED a significant increase in the level of N7-HOEtG could be detected in 9L cells following treatment with 5 microM CNU. Our study suggests that with this analytical method the formation of N7-HOEtG in the white blood cells of patients treated with chloroethylnitrosoureas may be determined.
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Brain Tumor Research Center, Department of Neurological Surgery, University of California, San Francisco 94143-0806, USA.
Bodell
W J
WJ
D000818:Animals; D000970:Antineoplastic Agents; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry; D018736:DNA Adducts / Q000032:analysis*; D004563:Electrochemistry; D005038:Ethylnitrosourea / Q000031:analogs & derivatives*; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D007739:L Cells; D051379:Mice; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000970:Antineoplastic Agents; D018736:DNA Adducts; C017139:1-(2-chloroethyl)-1-nitrosourea; C035018:N(7)-hydroxyethylguanine; D006147:Guanine; D004247:DNA; D005038:Ethylnitrosourea