title: Development and validation of a high-performance liquid chromatography method for the determination of cold relief ingredients in chewing gum.
abstract: An isocratic high-performance liquid chromatography (HPLC) method for the simultaneous determination of the active compounds paracetamol, pseudoephedrine and chlorpheniramine in chewing gum samples has been developed and validated. The method required a simple liquid-liquid extraction using n-hexane and a mixture of water-acetonitrile prior to HPLC analysis. The chromatographic separation was achieved with an aqueous solution containing hexylamine (pH 3)-acetonitrile as the mobile phase, a Spherisorb C18 column and UV detection at 220 nm. As an application, the proposed method has been used to evaluate the release into saliva of these compounds from samples under a controlled human chewing process.
authors.affiliation: Departamento de Química Analítica, Facultad de Química, Universidad Complutense, Madrid, Spain.
authors.forename: A I
authors.initials: AI
authors.lastname: Gasco-Lopez
authors.forename: R
authors.initials: R
authors.lastname: Izquierdo-Hornillos
authors.forename: A
authors.initials: A
authors.lastname: Jiminez
mesh_terms: D000082:Acetaminophen / Q000032:analysis*; D002638:Chewing Gum / Q000032:analysis*; D002744:Chlorpheniramine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004809:Ephedrine / Q000032:analysis*; D010919:Placebos; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002638:Chewing Gum; D010919:Placebos; D000082:Acetaminophen; D002744:Chlorpheniramine; D004809:Ephedrine