Combination of micropreparative capillary electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry for peptide analysis.
Signal suppression is a problem in matrix-assisted laser desorption/ionization mass spectrometry of peptides prepared by capillary electrophoresis. Many common electrolytes that are efficient for separation, such as sodium phosphate, also are strongly suppressive during laser desorption/ionization. We have tested individual electrolytes for highest performance in each step of separation and collection, respectively. Suppression is not observed if citrate, trifluoroacetic acid, or hydrochloric acid is used for collection, while phosphate still can be employed in the capillary providing excellent resolution. Low concentrations of hydrochloric acid added to the sample/matrix mixture generate mass spectra with better ion intensities than if trifluoroacetic acid or citrate is used.
Bergman
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Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. Bergman@mbb.ki.se
Bergman
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D019075:Electrophoresis, Capillary / Q000379:methods*; D008832:Microchemistry / Q000379:methods; D010455:Peptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D010455:Peptides