Transfection of reaggregating embryonic chicken retinal cells with an antisense 5'-DNA butyrylcholinesterase expression vector inhibits proliferation and alters morphogenesis.
The function of the enzyme butyrylcholinesterase (BChE) in the developing and mature brain is still unclear. We have inserted 577 bp of the 5' upstream region plus 106 bp of the exon 1 of the rabbit BChE gene in reverse orientation under control of an SV40 early promoter derivative in an expression vector. This vector was introduced by calcium phosphate-mediated transfection into embryonic chicken retina cells during the first days of reaggregation culture. Depending on the retinal origin, the transfected cell population forms histotypic retina-like spheres, so-called rosetted or stratified retinospheroids. We show that antisense 5'-BChE gene expression decreased the steady-state mRNA level of BChE and the translation of the BChE protein, inhibited proliferation, and accelerated histogenesis in both cellular systems. The pronounced effects of antisense 5'-BChE transfection of spheroids document a key role of BChE during the early reaggregation process of retinal cells, most likely by regulating their growth and differentiation.
Robitzki
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Department of Developmental and Neurobiology, Institute for Zoology, University of Technology, Darmstadt, Germany.
Mack
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Chatonnet
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D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000235:genetics*; D002449:Cell Aggregation; D002455:Cell Division; D002642:Chick Embryo; D016373:DNA, Antisense / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005822:Genetic Vectors; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D012160:Retina / Q000166:cytology / Q000196:embryology* / Q000201:enzymology*; D013539:Simian virus 40 / Q000235:genetics; D018874:Spheroids, Cellular / Q000378:metabolism; D014162:Transfection
D016373:DNA, Antisense; D012333:RNA, Messenger; D002091:Butyrylcholinesterase; D001973:Bromodeoxyuridine