Phenotypic changes of monocytes induced by HIV-1 gp120 molecule and its fragments.
Several phenotypic and functional changes of monocytes (M phi) have been described in HIV-1+ subjects and AIDS patients. Some of these changes that are pertinent for immunopathogenesis of the disease may be induced by HIV-1 envelope glycoprotein 120 (gp120). In the present study the effect of recombinant full length gp120 (FLgp120) and its two fragments: rp120cd (aa 410-511) and rp120 (aa 446-511) on the expression of the surface molecules of M phi cultured in vitro was determined. The FLgp120 and rp120cd caused upregulation of CD14 and CD44. The rp120cd peptide significantly increased the expression of CD16 (Fc gamma receptor type III) and TNF receptor type II. In contrast, the rp120 downregulated HLA-DR, CD64 (Fc gamma RI), interferon gamma receptor and induced IL-10 production by M phi. This study indicates that gp120 molecule and its fragments may induce several phenotypic changes of M phi in particular the increased proportion of CD14+CD16+ cells that is observed in the blood of AIDS patients. These results provide further evidence for variable response of M phi to gp120 which may explain the variability of phenotypic changes and heterogeneity of M phi subsets seen in HIV-1 disease.
Zembala
M
M
Department of Clinical Immunology, Jagiellonian University Medical School, Cracow, Poland. mizembal@cyf-kr.edu.pl
Bach
S
S
Szczepanek
A
A
Mancino
G
G
Colizzi
V
V
D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008262:Macrophage Activation / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D017452:Receptors, IgG / Q000096:biosynthesis; D017471:Receptors, Interferon / Q000096:biosynthesis; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis
D015815:Cell Adhesion Molecules; D015699:HIV Envelope Protein gp120; D000949:Histocompatibility Antigens Class II; D018950:Lipopolysaccharide Receptors; D010446:Peptide Fragments; D017452:Receptors, IgG; D017471:Receptors, Interferon; D018124:Receptors, Tumor Necrosis Factor; D016753:Interleukin-10; D007371:Interferon-gamma