Induction of premature mitosis in cells blocked in S-phase: possibilities of its use in cancer therapy.
Oncogenesis leads to weak regulations of many cell cycle checkpoints. This could be useful for cancer therapy. To test this assumption, five human malignant cell lines were used to induce premature mitosis by overriding the S-phase control, which ultimately leads to cell death. Two cell lines showed a significantly high frequency of premature mitotic cells induced by okadaic acid; whereas the others did not. One cell line, although having a high cyclin B level in cells blocked in S-phase, failed to show premature mitosis. The cyclin B level in cells blocked in S-phase alone could not explain the differential behavior of the cell lines. It was observed that in addition, the activation of cdc25 in these cells could also play an important role in the induction of premature mitosis in such cells.
Ghosh
S
S
Department of Botany, University of Calcutta, India.
Paweletz
N
N
Schroeter
D
D
D002448:Cell Adhesion / Q000187:drug effects; D016213:Cyclins / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008938:Mitosis / Q000187:drug effects; D019319:Okadaic Acid / Q000494:pharmacology*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D016196:S Phase / Q000187:drug effects; D013936:Thymidine / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology
D016213:Cyclins; D004791:Enzyme Inhibitors; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases; D013936:Thymidine