Conversion by Peyer's patch lymphocytes of human enterocytes into M cells that transport bacteria.
The epithelium that lines the gut is impermeable to macromolecules and microorganisms, except in Peyer's patches (PPs), where the lymphoid follicle-associated epithelium (FAE) contains M cells that transport antigens and microorganisms. A cultured system that reproduces the main characteristics of FAE and M cells was established by cultivation of PP lymphocytes with the differentiated human intestinal cell line Caco-2. Lymphocytes settled into the epithelial monolayer, inducing reorganization of the brush border and a temperature-dependent transport of particles and Vibrio cholerae. This model system could prove useful for intestinal physiology, vaccine research, and drug delivery studies.
Kernéis
S
S
Swiss Institute for Experimental Cancer Research, and Institute of Biochemistry, University of Lausanne, CH-1066 Epalinges-Lausanne, Switzerland.
Bogdanova
A
A
Kraehenbuhl
J P
JP
Pringault
E
E
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D018988:Bacterial Translocation; D018938:Caco-2 Cells; D002352:Carrier Proteins / Q000032:analysis; D016764:Cell Polarity; D018920:Coculture Techniques; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000166:cytology* / Q000276:immunology* / Q000382:microbiology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins / Q000032:analysis; D008863:Microspheres; D008871:Microvilli / Q000737:chemistry / Q000648:ultrastructure; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology*; D013394:Sucrase-Isomaltase Complex / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology; D013696:Temperature; D014734:Vibrio cholerae / Q000378:metabolism*
D002352:Carrier Proteins; D008840:Microfilament Proteins; C026615:villin; D013394:Sucrase-Isomaltase Complex; D016650:Fluorescein-5-isothiocyanate