Enhancing effect of interleukin-4 on the secretion of interferon-gamma by alpha s1-casein-specific CD8+ T cells.
alpha s1-Casein-specific CD8+ T cell clones expressed the interleukin (IL)-4 receptor, although they did not secrete detectable IL-4. We found that IL-4 significantly enhanced the secretion of interferon (IFN)-gamma by these CD8+ T cell clones. IL-4 also enhanced the secretion of IFN-gamma induced by stimulating the immobilized anti-CD3 antibodies of polyclonal CD8+ T cells which had been isolated from lymph nodes and were stimulated in vitro with the immobilized anti-CD3 antibody and IL-2. In addition, IL-4 added at the time of this first in vitro stimulation induced strong IFN-gamma productivity, as well as IL-4 and IL-10 productivity, which were detectable upon restimulation of these cells. Results are discussed in relation to the inhibitory effects of IFN-gamma production on IL-4-producing cells.
Nishijima
K
K
Department of Applied Biological Chemistry, University of Tokyo, Japan.
Kohyama
M
M
Hisatsune
T
T
Kato
H
H
Kakehi
M
M
Kaminogawa
S
S
D000818:Animals; D015703:Antigens, CD / Q000187:drug effects / Q000378:metabolism; D017252:CD3 Complex / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D002364:Caseins / Q000378:metabolism* / Q000494:pharmacology; D002999:Clone Cells; D005260:Female; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism*; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D018123:Receptors, Interleukin / Q000187:drug effects / Q000378:metabolism; D019948:Receptors, Interleukin-4
D015703:Antigens, CD; D017252:CD3 Complex; D002364:Caseins; D007376:Interleukin-2; D018123:Receptors, Interleukin; D019948:Receptors, Interleukin-4; D015847:Interleukin-4; D007371:Interferon-gamma