Molecular cloning and expression pattern of rpr-1, a resiniferatoxin-binding, phosphotriesterase-related protein, expressed in rat kidney tubules.
Bacterial phosphotriesterases are enzymes that hydrolyse phosphotriester-containing organophosphate pesticides. Resiniferatoxin is a vanilloid that desensitises nociceptive neurons. By screening a rat cDNA library with labelled resiniferatoxin, we unexpectedly isolated a novel rat phosphotriesterase homologue, here named rpr-1, that encodes a 349 amino acid, 39 kDa protein (confirmed by in vitro translation). Northern blotting and in situ hybridisation show expression primarily in proximal tubules of the kidney, in which rpr-1 distribution correlates with resiniferatoxin-binding activity. These results suggest an unsuspected link between the phosphotriesterase enzyme family and resiniferatoxin toxicity and pharmacology.
Davies
J A
JA
Department of Anatomy, University of Edinburgh Medical School, UK. jamie.davies@ed.ac.uk
Buchman
V L
VL
Krylova
O
O
Ninkina
N N
NN
D000595:Amino Acid Sequence; D000818:Animals; D043303:Aryldialkylphosphatase; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004224:Diterpenes / Q000378:metabolism*; D004950:Esterases / Q000737:chemistry*; D015870:Gene Expression; D047908:Intracellular Signaling Peptides and Proteins; D007684:Kidney Tubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000378:metabolism*; D051381:Rats; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D018076:DNA, Complementary; D004224:Diterpenes; D047908:Intracellular Signaling Peptides and Proteins; D009498:Neurotoxins; C107508:Pter protein, rat; C024353:resiniferatoxin; D004950:Esterases; D043303:Aryldialkylphosphatase