Detection and differentiation of primate alpha-herpesviruses by PCR.
A rapid method for detection and differentiation of 5 primate alpha-herpesviruses (human herpes simplex virus types 1 and 2 [HSV1, HSV2], green monkey simian agent 8, baboon herpesvirus 2 [HVP2], and macaque B virus [BV]) was developed utilizing the polymerase chain reaction (PCR). PCR primers were located in conserved regions of the gene encoding the glycoprotein B, which flanks an intervening region that is highly divergent among the 5 viruses. Amplified PCR products from the 5 viruses were readily differentiated by their unique restriction enzyme digestion patterns. No variation in digestion patterns was noted among strains of HSV1, HSV2, or HVP2. One clinical isolate of BV exhibited variation in a single restriction site, but its overall restriction pattern remained typical of BV. This method (PCR/RFLP) allowed the presence of herpesvirus DNA in clinical swabs from primates to be readily detected and the virus unambiguously identified.
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Department of Veterinary Infectious Diseases and Physiology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078-2006, USA.
Eberle
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D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D014644:Genetic Variation; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007438:Introns; D008251:Macaca; D010215:Papio; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification*; D014759:Viral Envelope Proteins / Q000235:genetics
D014759:Viral Envelope Proteins