title: Serodiagnosis of tuberculous meningitis by enzyme-linked immunosorbent assay (ELISA).
abstract: IgG antibodies against glycolipids and proteins isolated from M. tuberculosis and BCG suspension were determined by ELISA in sera, in CSFs and in serum and CSF paired samples, from patients with tuberculous meningitis and from healthy control subjects. With specificities between 90 and 94% for the antigens used, we obtained senitivities of 75% for Pr-ELISA, 60% for G1-ELISA and 35% for BCG-ELISA. As specific antibodies were detected in serum and CSFs, only one sample is enough to perform the test. We concluded that Pr-ELISA and G1-ELISA could be used as a supporting test in TBM diagnosis, especially when repeated bacteriological methods failed to prove the presence of tubercle bacilli and in cases without evidence of pulmonary tuberculosis.
authors.affiliation: Cantacuzino Institute, Bucharest, Romania.
authors.forename: D
authors.initials: D
authors.lastname: Niculescu
authors.forename: M
authors.initials: M
authors.lastname: Popa
authors.forename: H
authors.initials: H
authors.lastname: Stavri
authors.forename: I
authors.initials: I
authors.lastname: Teodor
authors.forename: D
authors.initials: D
authors.lastname: Stavri
authors.forename: O
authors.initials: O
authors.lastname: Dorobăţ
authors.forename: G
authors.initials: G
authors.lastname: Cozma
authors.forename: M
authors.initials: M
authors.lastname: Homoş
authors.forename: S
authors.initials: S
authors.lastname: Erşcoiu
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D009163:Mycobacterium bovis / Q000737:chemistry / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000276:immunology*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014390:Tuberculosis, Meningeal / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002556:Cerebrospinal Fluid Proteins; D006017:Glycolipids; D007074:Immunoglobulin G