A solubilization technique for photosensitizer quantification in ex vivo tissue samples.
The determination of the photosensitizer concentration in ex vivo tissue samples is commonly used for pharmacokinetic and dosimetric studies of photodynamic therapy, both clinically and pre-clinically. In this report, a new method is presented based on tissue solubilization and subsequent fluorometry. This method has the advantages of good sensitivity, accuracy and reproducibility, as well as low cost and ease of handling of the tissue samples. The method was tested for six different photosensitizers in a variety of tissues. The accuracy and concentration detection limits are compared with those of other published extraction methods.
Lilge
L
L
Ontario Cancer Institute, Toronto, Ont., Canada.
O'Carroll
C
C
Wilson
B C
BC
D000818:Animals; D004285:Dogs; D004947:Esophagus / Q000378:metabolism; D017324:Hematoporphyrin Derivative / Q000032:analysis / Q000493:pharmacokinetics; D006801:Humans; D007211:Indoles / Q000032:analysis / Q000493:pharmacokinetics; D009942:Organometallic Compounds / Q000032:analysis / Q000493:pharmacokinetics; D010777:Photochemistry / Q000379:methods*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000032:analysis* / Q000493:pharmacokinetics; D011166:Porphyrins / Q000032:analysis / Q000493:pharmacokinetics; D011524:Protoporphyrins / Q000032:analysis / Q000493:pharmacokinetics; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured
D007211:Indoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D011166:Porphyrins; D011524:Protoporphyrins; C052736:chloroaluminum tetrasulfophthalocyanine; C044797:chloroaluminum phthalocyanine; C063166:tin etiopurpurin; D017324:Hematoporphyrin Derivative; C028025:protoporphyrin IX