title: Polymorphonuclear leukocyte lysosomal proteases, cathepsins B and D affect the fibrinolytic system in human umbilical vein endothelial cells.
abstract: To clarify the physiological role played by neutrophil lysosomal protease in cultured human umbilical vein endothelial cells (HUVEC), we studied the effects of cathepsins B and D released from activated polymorphonuclear leukocytes on the fibrinolytic system in HUVEC. Cathepsins B and D reduced the antigens of tissue-type plasminogen activator, and they increased both the antigens and the activity of plasminogen activator inhibitor-1. These results suggest that cathepsins B and D are involved in the thrombotic tendency, since they inhibited the fibrinolytic system in cultured HUVEC.
authors.affiliation: New Drug Research Department, High Quality-Life Research Laboratories, Sumitomo Metal Industries, Souraku-gun, Kyoto, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Yokoi-Hayashi
mesh_terms: D002401:Cathepsin B / Q000494:pharmacology / Q000502:physiology*; D002402:Cathepsin D / Q000494:pharmacology / Q000502:physiology*; D002478:Cells, Cultured / Q000187:drug effects; D004730:Endothelium, Vascular / Q000502:physiology*; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology*; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis; D010959:Tissue Plasminogen Activator / Q000032:analysis
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D010959:Tissue Plasminogen Activator; D002401:Cathepsin B; D002402:Cathepsin D