Amphotericin B resistance testing of Candida spp.: a comparison of methods.
Several methods of susceptibility testing were compared for their ability to detect amphotericin B resistance among yeast isolates, including isolates known to be amphotericin-B-resistant in an animal model. A microtitre MIC method using antibiotic medium no. 3 and Etest strips were able to discriminate susceptible and resistant isolates. A disc susceptibility test was also able to identify resistant isolates and this method may be suitable for routine use as a screening test. A survey of resistance in 644 clinical isolates showed that the incidence of elevated MICs (> or = 0.25 mg/L) was only 1.2%.
Law
D
D
Department of Microbiology, University of Manchester, Hope Hospital, Salford, UK.
Moore
C B
CB
Denning
D W
DW
D000666:Amphotericin B / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D004352:Drug Resistance, Microbial; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012440:Saccharomyces / Q000187:drug effects
D000666:Amphotericin B