[White matter lesions after occlusion of the bilateral carotid arteries in the rat--temporal profile of cerebral blood flow (CBF), oligodendroglia and myelin].
In the present investigation, we examined cerebral blood flow (CBF), numerical density of oligodendroglia and extent of white matter lesions after bilateral ligation of common carotid arteries in Wistar rats. Doppler flow-meter revealed a reduction of CBF to 30-40% of that before operation after 1 and 3 days, however recovered to 50-60% after 7 and 14 days. Semiquantitative evaluation with immunohistochemistry for transferrin showed a numerical decrease of oligodendroglia in the medial corpus callosum after 14 and 30 days. Tissue rare-faction promptly occurred in the optic nerve and optic tract after 3 days, whereas it was delayed to 7 days after operation and increased in intensity gradually in the other white matter regions. These results indicate that bilateral occlusion of common carotid arteries in Wistar rats elicits a chronic cerebral hypoperfusion, which cumulatively leads to delayed appearance of white matter lesions.
Tomimoto
H
H
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
Akiguchi
I
I
Wakita
H
H
Kimura
J
J
D000818:Animals; D001921:Brain / Q000473:pathology*; D002545:Brain Ischemia / Q000473:pathology* / Q000503:physiopathology*; D017536:Carotid Artery, Common; D002452:Cell Count; D002560:Cerebrovascular Circulation / Q000502:physiology*; D008026:Ligation; D008297:Male; D009186:Myelin Sheath / Q000473:pathology*; D009836:Oligodendroglia / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar