Specific detection of mousepox virus by polymerase chain reaction.
Polymerase chain reaction was applied to the rapid identification and detection of mousepox virus. This was accomplished by selection of primers targeting the A-type inclusion body protein gene. By investigating 20 strains belonging to five different species of the genus Orthopoxvirus, amplification was achieved only with the seven mousepox virus strains examined. The size of the resulting DNA fragment accounted for 116 base pairs and contained a recognition site for the restriction enzyme HindII, thus confirming its viral origin. Amplification of mousepox virus specific sequences was also possible from infected mouse lung tissue and serum.
Neubauer
H
H
Institute of Microbiology, Federal Armed Forces Medical Academy, Munich, Germany.
Pfeffer
M
M
Meyer
H
H
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004481:Ectromelia virus / Q000235:genetics / Q000302:isolation & purification*; D004482:Ectromelia, Infectious / Q000175:diagnosis* / Q000821:virology; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D051379:Mice; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D014764:Viral Proteins / Q000235:genetics*
C088596:A-type inclusion protein, poxvirus; D017931:DNA Primers; D004279:DNA, Viral; D014764:Viral Proteins