title: Neo red cell as an organ preservation solution.
abstract: Neo red cell (NRC) was derived from outdated human red cells. The following experimental work has been done in order to investigate if NRC is valid for organ preservation. Hearts were obtained from male Lewis rat (250-350 g body weight). Heart transplantation was performed as Ono-Lindsey's method after 1, 3, 6, 12 and 24 hours simple cold storage. Rats were divided into two groups. Group 1; original NRC as preservation solution, Group 2; modified NRC (NRC suspended with UW solution) as preservation solution. After 1, 3, and 6 hour cold ischemic storage, the transplanted hearts in both groups showed contraction immediately after declamping the aorta and the pulmonary artery. After 12 and 24 hour preservation, the transplanted hearts in group 2 showed contraction. Myocardial ATP levels after 6, 12, 24 hours cold storage compared with 0 hour were 62.3%, 28.3%, 32.8% in group 1 respectively. On the contrary, myocardial ATP levels were 87.2%, 57.6%, 52.2% in group 2 respectively. After 24 hour preservation, pathological change was not remarkable between the two groups. Results of the prolonged preservation time and the myocardial ATP changes suggest that there is a possibility for effective use of NRC not only as blood substitute but also organ preservation solution.
authors.affiliation: Department of 3rd Surgery, Tokyo Womens' Medical College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Funakoshi
authors.forename: S
authors.initials: S
authors.lastname: Fujita
authors.forename: S
authors.initials: S
authors.lastname: Fuchinoue
authors.forename: T
authors.initials: T
authors.lastname: Agishi
authors.forename: K
authors.initials: K
authors.lastname: Ota
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D004912:Erythrocytes / Q000737:chemistry*; D016027:Heart Transplantation; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D019188:Organ Preservation Solutions / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D008081:Liposomes; D019188:Organ Preservation Solutions; D000255:Adenosine Triphosphate