Studies of the t-PA-catalyzed Glu-plasminogen activation on different models of polymeric fibrin.
The rate of activation of Glu-plasminogen by t-PA on fibrin clot and on the complexes of fibrinogen, its fragment X2, fibrin fragment D-D with N-DSK of fibrin has been studied. The reaction rate constant of (kcat/Km) increased and Km decreased in series of effectors X2-->X2 - N-DSK-->D-D-->D-D - N-DSK = fibrinogen - N-DSK-->fibrin clots. The structure of activator complexes of reaction of Glu-plasminogen activation by t-PA on fibrin clot has been suggested.
Druzhyna
N N
NN
Department of Structure and Functions of Proteins, Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kiev, Ukraine.
Makogonenko
E M
EM
Yakovlev
S A
SA
D005337:Fibrin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D010958:Plasminogen / Q000378:metabolism*; D011108:Polymers; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
D011108:Polymers; D005337:Fibrin; D010958:Plasminogen; D010959:Tissue Plasminogen Activator