Developmental regulation of yolk protein gene expression in Anastrepha suspensa.
A partial cDNA clone for the 48,000 dalton yolk polypeptide gene from Anastrepha suspensa was isolated from a cDNA expression library using a yolk polypeptide antibody probe and hybridization to the Drosophila melanogaster yolk protein 1 gene. The sequenced DNA has greatest homology to the yolk protein genes from Ceratitis capitata, D. melanogaster, and Calliphora erythrocephala and, similar to these genes, shares amino acid sequence domains with those from lipases. RNA hybridization studies indicated that the yolk protein gene expression is completely female-specific and limited to the ovaries, without apparent regulation by 20-hydroxyecdysone or juvenile hormone. This is in contrast to an earlier study which suggested, based on immunological probes, that a very low level of yolk protein synthesis occurred in fat body and was not sex-specific.
Handler
A M
AM
Center for Medical, Agricultural, and Veterinary Entomology, US Department of Agriculture, Gainesville, Florida 32608, USA. handler@nervm.nerdc.ufl.edu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004175:Diptera / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics; D004441:Ecdysterone / Q000494:pharmacology; D004527:Egg Proteins / Q000235:genetics*; D005216:Fat Body / Q000187:drug effects / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D007605:Juvenile Hormones / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014818:Vitellogenesis / Q000235:genetics; D014819:Vitellogenins / Q000235:genetics
D018076:DNA, Complementary; D004527:Egg Proteins; D019476:Insect Proteins; D007605:Juvenile Hormones; D014819:Vitellogenins; D004441:Ecdysterone; D012313:RNA