Identification by polymerase chain reaction and hybridization with sequence-specific oligoprobes and complete exon-2 cDNA sequencing of a new DRB1 (DRB1*1131) allele.
HLA-DRB1 is the most polymorphic gene described so far, and their encoded molecules disclose a major role in allogeneic responses. We describe in this report a new DRB1 allele in a Spanish Caucasian bone marrow donor, initially defined by PCR-SSO as a DRB1*11-like allele. Complete exon 2 cDNA-sequencing reveled that this allele was identical to DRB1*1119 except for a single substitution at position 178, which generates an amino acid change (Tyr-His) at position 60. This residue is shared by several DRB1*14 subtypes and DRB1*0808. The new allele was officially named DRB1*1131.
Balas
A
A
Department of Histocompatibility, Regional Transfusion Centre, Madrid, Spain. ctm@bitmailer.net
Santos
S
S
Garciá-Sánchez
F
F
Garciá-Escribano
C
C
Merino
J L
JL
Vicario
J L
JL
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002461:Cell Line, Transformed; D018076:DNA, Complementary; D005091:Exons; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D015345:Oligonucleotide Probes