Chromosome Microdissection-Based Techniques for Genome Analysis
It is now possible to start at a cytogenetically defined position in any eucaryotic genome and proceed toward isolation and identification of candidate genes known to map to that position, taking advantage of the new PCR amplification technology to produce position-specific DNA. The starting material for this very useful exercise is DNA microdissected from standard cytogenetic preparations. Here we describe techniques for acquiring useful samples of such DNA and strategies for the use of chromosome region-specific DNA for gene discovery projects.
Hozier
JC
Applied Genetics Laboratories, Inc., 1335 Gateway Drive, Suite 2001, Melbourne, Florida, 32901
Hall
BK
Sims
KR
Liechty
MC
Chen-Liu
L
Davis
LM