title: Large scale and clinical grade purification of syndecan-1+ malignant plasma cells.
abstract: For cancer immunotherapy, it is usually necessary to obtain a large number of tumor cells from patients. We have previously reported that syndecan-I is present only on malignant plasma cells in samples from patients with multiple myelomatosis. We report here that this antigen is cleaved by chymopapain. This makes it possible to develop a rapid and clinical grade procedure to purify large numbers of myeloma cells using anti-syndecan-1 mAb, magnetic beads and chymopapain.
authors.affiliation: Unit for Cellular Therapy, 3 Hôpital St. Eloi, Montpellier, France.
authors.forename: R X
authors.initials: RX
authors.lastname: Sun
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Lu
authors.forename: J
authors.initials: J
authors.lastname: Wijdenes
authors.forename: J
authors.initials: J
authors.lastname: Brochier
authors.forename: C
authors.initials: C
authors.lastname: Hertog
authors.forename: J F
authors.initials: JF
authors.lastname: Rossi
authors.forename: B
authors.initials: B
authors.lastname: Klein
mesh_terms: D002917:Chymopapain; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008562:Membrane Glycoproteins; D009101:Multiple Myeloma / Q000473:pathology*; D010950:Plasma Cells / Q000276:immunology / Q000473:pathology*; D011509:Proteoglycans; D053668:Syndecan-1; D053667:Syndecans
chemical_list: D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508241:SDC1 protein, human; D053668:Syndecan-1; D053667:Syndecans; D002917:Chymopapain