Reinfection-induced increase of rubella persistently infected cells in a macrophage-like cell line.
The establishment and relevant characteristics of a long-term rubella persistent infected immortalised murine macrophage culture with a high proportion of persistent infected cells over uninfected is described. The high proportion of persistent infected macrophages was obtained through reinfection with the original virus. Prior to reinfection, 35-50% of the cells expressed viral antigen, 0.05-1.5% produced infective virus and extra-cellular virus was continuously produced with infective titers cyclically fluctuating between 10(2) and 10(7) TCID50/ml. After reinfection, the macrophages which expressed viral antigen and produced infective virus had increased to 75-90% and 30-45% respectively and extracellular virus was continuously produced with stable titer between 10(2)-10(3) TCID50/ml. Furthermore, in the reinfected culture no significant variation was observed in the percentage of cells expressing antigen, producing virus and in the titer of extracellular virus for longer than a year of passages.
Sarmiento
R E
RE
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México.
Tirado
R
R
Gómez
B
B
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis; D007941:Leukemia P388; D008264:Macrophages / Q000276:immunology / Q000821:virology*; D051379:Mice; D012409:Rubella / Q000276:immunology / Q000473:pathology / Q000821:virology*; D012412:Rubella virus / Q000254:growth & development* / Q000276:immunology
D000956:Antigens, Viral