Characterization of a diamine exporter in Chinese hamster ovary cells and identification of specific polyamine substrates.
Export of the diamine putrescine was studied using inside-out plasma membrane vesicles prepared from Chinese hamster cells. Putrescine uptake into vesicles was a saturable and an ATP- and antizyme-independent process. Excess amounts of a series of diamines or monoacetyl spermidine, but not monoacetyl putrescine, spermidine, or spermine, inhibited putrescine transport. Putrescine uptake into vesicles prepared at pH 7.4 was suppressed at pH 5, compared with pH 7.4; was stimulated approximately 2.5-fold at pH 7.4 in vesicles prepared at pH 6.25, compared with vesicles prepared at pH 7.4; and was not inhibited by valinomycin in the presence of potassium ions. Reserpine and verapamil blocked [3H]putrescine uptake into inverted vesicles. Verapamil treatment caused an increase in intracellular contents of putrescine, cadaverine, and N8-acetylspermidine, in unstressed proliferating cells, or of N1-acetylspermidine, in cells subjected to heat shock to induce acetylation of spermidine at N1. These data indicate that putrescine export in Chinese hamster cells is mediated by a non-electrogenic antiporter capable of using protons as the counter ion. Physiological substrates for this exporter include putrescine, cadaverine, and monoacetyl spermidine and have the general structure NH3+-(CH2)n-NH2 + R at acidic or neutral pH.
Xie
X
X
Committee on Cancer Biology, Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USA.
Gillies
R J
RJ
Gerner
E W
EW
D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D006224:Cricetinae; D006863:Hydrogen-Ion Concentration; D011700:Putrescine / Q000378:metabolism*; D012110:Reserpine / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D014634:Valinomycin; D012110:Reserpine; D014700:Verapamil; D011700:Putrescine