p53 gene mutation in N-butyl-N-(4-hydroxybutyl)nitrosamine-induced urinary bladder tumors and N-methyl-N-nitrosourea-induced colon tumors of rats.
We analyzed p53 mutations in 17 N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder transitional cell carcinomas (TCCs) with or without areas of squamous cell carcinoma (SCC) of Long-Evans Cinnamon (LEC) and F344 rats, and in 7 N-methyl-N-nitrosourea-induced colon adenocarcinomas of LEC rats by polymerase chain reaction-single strand conformation polymorphism analysis and DNA sequencing. Of these bladder tumors, one TCC with moderately differentiated SCC had a T to G transversion mutation at codon 141, leading to a Val to Gly amino acid change. No p53 mutation was found in colon adenocarcinomas. Thus a p53 gene mutation seems infrequent in these rat bladder and colon carcinogenesis models even in the late stage.
Min
H
H
Second Department of Pathology, School of Medicine, The University of Tokushima, Kuramoto-cho, Japan.
Kudo
E
E
Hino
A
A
Yoshimoto
K
K
Iwahana
H
H
Itakura
M
M
Izumi
K
K
D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D002295:Carcinoma, Transitional Cell / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics*; D005091:Exons; D016158:Genes, p53; D008297:Male; D008770:Methylnitrosourea; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D051381:Rats; D011916:Rats, Inbred F344; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D002085:Butylhydroxybutylnitrosamine; D008770:Methylnitrosourea