Facilitated oxygen transport with modified and encapsulated hemoglobins across non-flowing solution membrane.
The oxygen-transporting capability of modified and encapsulated hemoglobins and red cells is discussed from a physico-chemical standpoint in order to design oxygen-delivering fluids. The oxygen diffusion coefficient toward oxygen-deficient sites was estimated by measuring the oxygen flux across thin solution membranes of hemoglobin, polymerized hemoglobin, liposome-encapsulated hemoglobin, and red cells. Oxygen flux was enhanced several times over that of nitrogen for the hemoglobin and red cell solution with ca [Hb] = 10 and 15 g/dl, respectively. The enhancement in the oxygen diffusion is ascribed to the facilitated transport of oxygen via the hemoglobins. This was in contrast to the simple and physical oxygen-diffusivity in response to its concentration gradient, in the absence of hemoglobins. The flux of the oxygen transport was in the order of hemoglobin > red cells > polymerized hemoglobin > encapsulated hemoglobin, which was ascribed to the facilitated transport efficiencies of oxygen with hemoglobins in a non-flowing or stationary solution.
Nishide
H
H
Department of Polymer Chemistry, Waseda University, Tokyo, Japan.
Chen
X S
XS
Tsuchida
E
E
D000818:Animals; D001692:Biological Transport; D001802:Blood Substitutes / Q000378:metabolism*; D002417:Cattle; D004910:Erythrocyte Membrane / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D008567:Membranes, Artificial; D008954:Models, Biological; D010100:Oxygen / Q000097:blood*; D012996:Solutions
D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes; D008567:Membranes, Artificial; D012996:Solutions; D010100:Oxygen