Block of the cell cycle of the yeast Saccharomyces cerevisiae by tyrphostin, an inhibitor of protein tyrosine kinase.
Tyrphostins are inhibitors of the epidermal growth factor receptor tyrosine kinase. To elucidate the biological function of protein tyrosine kinases in yeast cells, a mutant hypersensitive to tyrphostin was isolated and investigated for its response to the drug. The mutation was recessive and was designated tpt1 for tyrphostin hypersensitive. A tpt1 strain cannot grow in the presence of tyrphostin, implying that a biological process sensitive to tyrphostin is essential for cell growth. Microscopic observation indicated that large-budded cells were accumulated in the presence of the inhibitor. The results suggest the involvement of protein tyrosine phosphorylation in the cell cycle progression of Saccharomyces cerevisiae.
Fujimura
H
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Central Research Laboratory, Nippon Hoechst Marion Roussel, Kawagoe, Japan.
D000935:Antifungal Agents / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects*; D003513:Cycloheximide / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D009154:Mutation; D009570:Nitriles / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D009570:Nitriles; D003513:Cycloheximide; D011505:Protein-Tyrosine Kinases