title: cut-1-like genes of Ascaris lumbricoides.
abstract: Three genomic fragments homologous to cut-1 of Caenorhabditis elegans (C. elegans) have been identified in the intestinal parasitic nematode Ascaris lumbricoides (A. lumbricoides). Two of these fragments identify one region of the A. lumbricoides genome; they are separated by 8-9 kb and have opposite orientation, with the direction of transcription converging toward the center of the region. The third gene, which has been studied more completely, is in a different region of the genome separated from the first one by not less than 12-15 kb. The complete genomic sequence of this third gene has been determined. cDNA overlapping clones were obtained from adult A. lumbricoides RNA via the rapid amplification of cDNA ends (RACE) procedure [Frohman et al., 1988. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002] and sequenced. The mature mRNA of this gene, which we have named ascut-1, is trans-spliced to the spliced leader sequence of nematodes (SL1) [Krause, M., Hirsh, D., 1987. A trans-spliced leader sequence on actin mRNA in C. elegans. Cell 49, 753-761]. The mRNA is 1684 nt long plus the poly(A) tail and contains four exons with a 138 nt untranslated 5' leader and a 388 nt untranslated 3' tail. Conceptual translation of the coding sequence shows a protein of 385 aa with a signal peptide of 16 aa. The protein shows very high homology with CECUT-1, the product of the C. elegans gene cut-1 and with other cuticlin proteins of nematodes. A 262 amino acids region which is strongly conserved between these proteins seems to identify a group of proteins, so far restricted to nematodes, for which the name CUT-1-like is proposed.
authors.affiliation: Institut Pasteur du Maroc, Casablanca, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: Timinouni
authors.forename: P
authors.initials: P
authors.lastname: Bazzicalupo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017164:Ascaris lumbricoides / Q000235:genetics*; D001483:Base Sequence; D029742:Caenorhabditis elegans Proteins; D017238:Genes, Helminth; D015698:Genomic Library; D015801:Helminth Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D018510:RNA, Helminth / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D018510:RNA, Helminth; D012333:RNA, Messenger; C070207:CUT-1 protein, C elegans