title: Donor peripheral blood stem cell infusions in recipients of living-related liver allografts.
abstract: BACKGROUND
  In this pilot study, donor peripheral blood stem cell (DPBSC) infusions were performed in three recipients of living-related liver transplants (LRLT).
  
  
  METHODS
  DPBSCs were obtained by leukapheresis after mobilization with granulocyte-colony-stimulating factor (Filgrastim). Donor leukapheresis was performed on the 5th postoperative day, and half of the DPBSCs were infused into the recipient on the day of collection. The second half of the pheresed product was cryopreserved for delayed administration.
  
  
  RESULTS
  Results from preliminary studies of chimerism in LRLT recipients, at 20 weeks posttransplant, suggested that the levels of donor cells detected in LRLT recipients treated with DPBSC infusions may be higher than those observed for recipients of cadaver donor liver allografts and vertebral body marrow infusions.
  
  
  CONCLUSIONS
  The results of this pilot study indicate that administration of mobilized DPBSC to recipients of LRLT is a feasible procedure for both donor and recipient.
authors.affiliation: Department of Surgery, University of Miami School of Medicine, Florida 33136, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Tsaroucha
authors.forename: C
authors.initials: C
authors.lastname: Ricordi
authors.forename: T A
authors.initials: TA
authors.lastname: Noto
authors.forename: N S
authors.initials: NS
authors.lastname: Kenyon
authors.forename: R
authors.initials: R
authors.lastname: Garcia-Morales
authors.forename: J R
authors.initials: JR
authors.lastname: Nery
authors.forename: J
authors.initials: J
authors.lastname: Miller
authors.forename: A G
authors.initials: AG
authors.lastname: Tzakis
mesh_terms: D000328:Adult; D001782:Blood Donors; D001793:Blood Preservation; D002675:Child, Preschool; D015925:Cryopreservation; D005260:Female; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation / Q000706:statistics & numerical data*; D006801:Humans; D007223:Infant; D007937:Leukapheresis; D007963:Leukocytes, Mononuclear / Q000166:cytology; D016031:Liver Transplantation / Q000401:mortality*; D019520:Living Donors; D010865:Pilot Projects; D011184:Postoperative Period