Histamine does not potentiate cyclic AMP-mediated amylase secretion in the guinea-pig pancreatic acinar cells.
1. This study investigates the interaction between histamine and the adenylate cyclase systems involved in the secretion of amylase in isolated guinea-pig pancreatic acinar cells. 2. Histamine caused does-related enhancement of amylase release. Similarly, incubation of acini with increasing concentrations of vasoactive intestinal peptide (VIP) resulted in a typical dose-dependent increase in amylase output. 3. When pancreatic acinar cells were incubated with histamine in combination with VIP, amylase secretion did not differ statistically from secretion induced by histamine or VIP alone and was significantly lower than theoretical additivity. Additionally, amylase secretion in the presence of histamine plus forskolin was significantly less than additive. The action of histamine was equally effective as VIP in causing cyclic adenosine monophosphate (cAMP) increase. 4. These results indicate that histamine may exert its secretory effects via the cyclic AMP pathway in the exocrine guinea-pig pancreas.
Camello
C
C
Department of Physiology, Faculty of Veterinary Science, University of Extremadura, Caceres, Spain.
Lajas
A I
AI
Gonzalez
A
A
Camello
P J
PJ
Pariente
J A
JA
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D010179:Pancreas / Q000187:drug effects* / Q000201:enzymology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D006632:Histamine; D000242:Cyclic AMP; D000681:Amylases