title: Improved in vitro cultivation of Babesia caballi.
abstract: Babesia caballi infected erythrocytes were collected from the blood of an experimentally infected horse and could be continuously cultivated in vitro with parasitemia ranging from 2-4% in RPMI 1640 medium supplemented with 2 mM L-glutamine, 20 mM HEPES and 40% adult horse serum in a low oxygen atmosphere (2% O2, 5% CO2 and 93% N2). All attempts to increase parasitemia failed using other culture media, serum concentrations and culture vessels. However, parasite growth was enhanced by transfer of cultures from a low oxygen to 5% CO2 in air, with parasitemia ranging from 8-10%.
authors.affiliation: Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Avarzed
authors.forename: I
authors.initials: I
authors.lastname: Igarashi
authors.forename: T
authors.initials: T
authors.lastname: Kanemaru
authors.forename: K
authors.initials: K
authors.lastname: Hirumi
authors.forename: Y
authors.initials: Y
authors.lastname: Omata
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Oyamada
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001403:Babesia / Q000254:growth & development* / Q000302:isolation & purification; D001404:Babesiosis / Q000469:parasitology*; D002478:Cells, Cultured; D003470:Culture Media; D004912:Erythrocytes / Q000469:parasitology*; D006734:Horse Diseases / Q000469:parasitology*; D006736:Horses / Q000469:parasitology*; D066298:In Vitro Techniques; D018512:Parasitemia; D010274:Parasitology / Q000379:methods
chemical_list: D003470:Culture Media