Purification of the transcription/repair factor TFIIH and evaluation of its associated activities in vitro.
We describe here the methodology developed in our laboratory to study the role of TFIIH, a multisubunit protein complex, in the various mechanisms of cell life: transcription, DNA repair, and cell cycle regulation. Protocols are given to purify TFIIH and to study its various enzymatic activities as well as its transcription and nucleotide excision repair activities.
Marinoni
J C
JC
Institut de Biologie et Génétique Moléculaire et Cellulaire, UPR 6520 (CNRS), Unité 184 (INSERM), Illkirch, France.
Rossignol
M
M
Egly
J M
JM
D004260:DNA Repair; D006367:HeLa Cells; D006801:Humans; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH