title: Time-dependent increases in syndecan-1 and fibroglycan messenger RNA expression in the infarct zone after experimentally induced myocardial infarction in rats.
abstract: BACKGROUND
  Syndecan-1 and fibroglycan, heparan sulphate proteoglycans, play important roles in extracellular matrix formation via their biological functions.
  
  
  OBJECTIVE
  To examine experimentally the sequential changes in syndecan-1 and fibroglycan messenger RNA (mRNA) expression after acute myocardial infarction.
  
  
  METHODS
  The left coronary arteries of male Sprague-Dawley rats were ligated and the hearts were excised on days 1-14, 28 and 42. Syndecan-1 and fibroglycan mRNA expression in the infarct and non-infarct zones and in sham-operated hearts was determined by reverse transcriptase-polymerase chain reaction. Amplified products were quantified by densitometry of the electrophoresed bands stained with ethidium bromide and standardized relative to the glyceraldehyde 3-phosphate dehydrogenase or beta-actin mRNA expression. Northern hybridization was also performed in the infarct and non-infarct zones on day 3.
  
  
  RESULTS
  Expression both of syndecan-1 and of fibroglycan mRNA began to increase on day 2. The expression attained maximum levels on day 3. The maximum levels of syndecan-1 and fibroglycan expression were, respectively, sevenfold and fivefold the preligation level and the level in the sham-operated hearts. The levels remained elevated until day 14, whereupon they declined gradually, returning to the control levels by around day 42. Northern blotting also demonstrated that there was an increased expression both of syndecan-1 and of fibroglycan mRNA in the infarct compared with that in the non-infarct zone on day 3.
  
  
  CONCLUSIONS
  Our results demonstrated that there are sequential increases in the expression both of syndecan-1 and of fibroglycan mRNA in the infarct zone after experimentally induced myocardial infarction in rats, suggesting that these proteoglycans play some role in the pathological course of infarction.
authors.affiliation: First Department of Internal Medicine, Okayama University Medical School, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Endo
authors.forename: S
authors.initials: S
authors.lastname: Kusachi
authors.forename: Y
authors.initials: Y
authors.lastname: Ninomiya
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Shinji
authors.forename: N
authors.initials: N
authors.lastname: Koide
authors.forename: J
authors.initials: J
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D015415:Biomarkers; D015152:Blotting, Northern; D017931:DNA Primers / Q000737:chemistry; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009203:Myocardial Infarction / Q000209:etiology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053668:Syndecan-1; D053669:Syndecan-2; D053667:Syndecans
chemical_list: D015415:Biomarkers; D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D012333:RNA, Messenger; C508243:Sdc1 protein, rat; C508247:Sdc2 protein, rat; D053668:Syndecan-1; D053667:Syndecans; D053669:Syndecan-2