title: Pulse schemes for the measurement of 3JC'C gamma and 3JNC gamma scalar couplings in 15N,13C uniformly labeled proteins.
abstract: Pulse sequences are presented for the measurement of 3JC'C gamma and 3JNC gamma scalar couplings for all C gamma containing residues in 15N,13C uniformly labeled proteins. The methods described are based on quantitative J correlation spectroscopy pioneered by Bax and co-workers [Bax et. al. (1994) Methods Enzymol., 239, 79-105]. The combination of 3JC'C gamma and 3JNC gamma scalar coupling constants allows the assignment of discrete rotameric states about the chi 1 torsion angle in cases where such states exist or, alternatively, facilitates the establishment of noncanonical chi 1 conformations or the presence of rotameric averaging. The methods are applied to a 1.5 mM sample of staphylococcal nuclease.
authors.affiliation: Protein Engineering Network of Centres of Excellence, University of Toronto, ON, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Konrat
authors.forename: D R
authors.initials: DR
authors.lastname: Muhandiram
authors.forename: N A
authors.initials: NA
authors.lastname: Farrow
authors.forename: L E
authors.initials: LE
authors.lastname: Kay
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008836:Micrococcal Nuclease / Q000737:chemistry*; D008956:Models, Chemical; D009587:Nitrogen Isotopes; D011487:Protein Conformation
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D008836:Micrococcal Nuclease