title: Transferred nuclear Overhauser effect spectroscopy study of a peptide from the PapG pilus subunit bound by the Escherichia coli PapD chaperone.
abstract: Interaction of the Escherichia coli PapD chaperone with the synthetic peptide PapG308-314 (Thr-Met-Val-Leu-Ser-Phe-Pro), corresponding to the seven C-terminal residues of the PapG pilus subunit, was studied by transferred nuclear Overhauser effect (TRNOE) spectroscopy. The observation of cross-peaks corresponding to either intraresidue or sequential C(alpha)H/NH and C(beta)H/NH TRNOEs and the absence of sequential NH(i)/NH(i+1) TRNOEs indicate that the peptide binds to PapD in an extended conformation. In addition, line-broadening effects gave information of the peptide's mode of interaction with PapD. These observations were in excellent agreement with a recent crystal structure of a PapG peptide complexed with PapD.
authors.affiliation: Physical Chemistry 2, Center for Chemistry and Chemical Engineering, Lund University, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Walse
authors.forename: J
authors.initials: J
authors.lastname: Kihlberg
authors.forename: K F
authors.initials: KF
authors.lastname: Karlsson
authors.forename: M
authors.initials: M
authors.lastname: Nilsson
authors.forename: K G
authors.initials: KG
authors.lastname: Wahlund
authors.forename: J S
authors.initials: JS
authors.lastname: Pinkner
authors.forename: S J
authors.initials: SJ
authors.lastname: Hultgren
authors.forename: T
authors.initials: T
authors.lastname: Drakenberg
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D018832:Molecular Chaperones; D015394:Molecular Structure; D033903:Periplasmic Proteins; D011487:Protein Conformation
chemical_list: D018830:Adhesins, Escherichia coli; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; C084330:PapD protein, E coli; C092426:PapG protein, E coli; D033903:Periplasmic Proteins; D036781:Fimbriae Proteins