title: Reduction of thymine hydroperoxide by phospholipid hydroperoxide glutathione peroxidase and glutathione transferases.
abstract: Thymine hydroperoxide (5-hydroperoxymethyluracil), a model compound representing products of oxidative damage to DNA, is a substrate for glutathione peroxidase and some isoforms of glutathione transferase. In this paper, we show that selenium-dependent human phospholipid hydroperoxide glutathione peroxidase (Se-PHGPx) exhibits about four orders of magnitude higher activity on thymine hydroperoxide than that of other human enzymes such as selenium-dependent glutathione peroxidase and various representatives of glutathione transferases. The results indicate that Se-PHGPx may be an important enzyme in repairing oxidatively damaged DNA.
authors.affiliation: Department of Biochemistry, Institute of Food Research, Norwich Laboratory, Norwich Research Park, UK.
authors.forename: Y
authors.initials: Y
authors.lastname: Bao
authors.forename: P
authors.initials: P
authors.lastname: Jemth
authors.forename: B
authors.initials: B
authors.lastname: Mannervik
authors.forename: G
authors.initials: G
authors.lastname: Williamson
mesh_terms: D000818:Animals; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D051381:Rats; D013941:Thymine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: C011851:5-hydroperoxymethyluracil; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D013941:Thymine