title: Increased levels of mRNA for beta- but not alpha-subunit of calmodulin kinase II following kindled seizures.
abstract: We studied levels of mRNA for the alpha- and beta-subunits of calmodulin (CaM) kinase II using the amygdaloid kindling model of epilepsy. There were significant increases in mRNA for the beta-subunit of CaM kinase II in the hippocampus 4-24 h after stage 5-kindled seizures. Moreover, this mRNA was significantly increased by 20.0-26.5% in the bilateral dentate gyrus 8 to 24 h after kindled seizures. The beta-subunit mRNA was also significantly increased by 13.5-19.0% in the CA3 on the side ipsilateral to the stimulation, 4 to 8 h after kindled seizures. mRNA for the alpha-subunit of CaM kinase II was not significantly changed in the regions examined for up to 24 h after the kindled seizures. These results suggest that CaM kinase II mediates the molecular processes that follow kindled seizures. It is possible that increases in CaM kinase II-dependent protein phosphorylation are associated with the plastic changes in kindling.
authors.affiliation: Department of Neuropsychiatry, Faculty of Medicine, Kagawa Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Morimoto
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Kashihara
authors.forename: T
authors.initials: T
authors.lastname: Hayabara
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D004195:Disease Models, Animal; D004827:Epilepsy / Q000201:enzymology*; D017403:In Situ Hybridization; D007696:Kindling, Neurologic; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D012333:RNA, Messenger; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases