title: Detection and characterization of Leishmania in tissues of patients with post kala-azar dermal leishmaniasis using a specific monoclonal antibody.
abstract: Sections from skin lesions and draining lymph nodes of patients with post kala-azar dermal leishmaniasis were examined using an immunoperoxidase method and a monoclonal antibody directed against Leishmania donovani. Parasites were detected in 22 of 25 biopsies (88%). In parallel sections stained by haematoxylin and eosin, parasites were detected in only 5 of 25 of the biopsies (20%). The demonstration of whole parasites or parasite antigen is diagnostic.
authors.affiliation: Centre of Medical Parasitology, University of Copenhagen, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Ismail
authors.forename: A
authors.initials: A
authors.lastname: Kharazmi
authors.forename: H
authors.initials: H
authors.lastname: Permin
authors.forename: A M
authors.initials: AM
authors.lastname: el Hassan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007893:Leishmania donovani / Q000276:immunology / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D007898:Leishmaniasis, Visceral / Q000150:complications*; D008198:Lymph Nodes / Q000469:parasitology; D012867:Skin / Q000469:parasitology
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan