[Diagnostic value of hematopoietic stem cells].
Proliferation and differentiation of the haemopoietic stem cell are critical for the maintenance of normal haemopoiesis, damage to haemopoiesis, onset of malignant haemopoiesis and repopulation of haemopoiesis after bone marrow transplantation. To evaluate the actual state of haemopoiesis a quantitative assay of haemopoietic stem cells would be needed. Morphology of haemopoietic stem cells cannot be detected by their microscopic morphology. In human practice no stem cell assay is available, therefore, the quantity of human pluripotent stem cells is characterised by the frequency of the progenitors differentiating into erythrocyte, granulocyte or macrophage cell lines (CFU-GEMM, BFU-E, CFU-GM, CFU-Meg). Progenitor cells, can be easily tested by their ability to form in vitro colonies in soft gel cultures in the constant presence of the specific regulator of the cell line ("colony stimulating factor" = CSF). Seven-14 days after explantation of haemopoietic cells colonies containing 40 to 1000 cells are formed. These colonies are proved to be of clonal origin. The quantitative assay of colony forming progenitors has a critical significance in haematological diseases due stem cell damage and bone marrow transplantation. The present paper deals with the diagnostic value of progenitor assays.
Gidáli
J
J
Országos Hematológiai és Immunológiai Intézet, Budapest.
Fehér
I
I
D000741:Anemia, Aplastic / Q000097:blood* / Q000628:therapy; D016026:Bone Marrow Transplantation; D003115:Colony-Stimulating Factors; D006402:Hematologic Diseases / Q000097:blood* / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D009196:Myeloproliferative Disorders / Q000097:blood / Q000628:therapy; D009436:Neural Tube Defects / Q000097:blood / Q000628:therapy
D003115:Colony-Stimulating Factors
A vérképzö elödsejtek vizsgálatának diagnosztikai jelentösége.