Identification of wallaby milk whey proteins separated by two-dimensional electrophoresis, using amino acid analysis and sequence tagging.
Micropreparative two-dimensional polyacrylamide gel electrophoresis has been used to separate milk whey proteins from the Tammar wallaby (Macropus eugenii). We have used a combination of amino acid analysis and N-terminal sequence tagging as a rapid and sensitive method to identify the major whey proteins. Using these techniques, we confidently identified alpha-lactalbumin and late lactation protein. While these are the only two M. eugenii whey proteins with a corresponding SWISS-PROT entry, we demonstrate that by using amino acid analysis and matching across species boundaries, we can identify previously unsequenced conserved wallaby whey proteins including beta-lactoglobulin and serum albumin.
Molloy
M P
MP
Australian Proteome Analysis Facility (APAF), Macquarie University, Sydney, NSW.
Herbert
B R
BR
Yan
J X
JX
Williams
K L
KL
Gooley
A A
AA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007768:Lactalbumin / Q000032:analysis; D007614:Macropodidae; D008894:Milk Proteins / Q000032:analysis* / Q000737:chemistry; D008969:Molecular Sequence Data; D017421:Sequence Analysis / Q000379:methods; D000067816:Whey Proteins
D000596:Amino Acids; D008894:Milk Proteins; D000067816:Whey Proteins; D007768:Lactalbumin