title: The bovine guanylate cyclase GC-E gene and 5' flanking region.
abstract: The gene encoding the bovine guanylate cyclase isoform E (GC-E) was isolated as a single 18 kb genomic clone and shown to have 20 exons and 19 introns. Comparison of the structure of the GC-E gene with structures of other membrane guanylate cyclase genes indicates that the GC-E is most closely related to the subfamily of sensory guanylate cyclases. Comparison of the GC-E structure with that of the more distantly related guanylate cyclase isoform A (GC-A) gene shows the most divergence in the extracellular and C-terminal regions, but general conservation of introns and exons in the intracellular kinase-like and catalytic domains. RT-PCR from several bovine tissues shows that GC-E is expressed only in the retina. Consistent with this pattern of expression, elements for the retinal-specific transcription factors RET-1, RET-2 and Talpha-1 are located in the 5' flanking promoter region.
authors.affiliation: Department of Ophthalmology, Harvard Medical School/MEEI, Boston, MA 02114, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Johnston
authors.forename: F
authors.initials: F
authors.lastname: Farhangfar
authors.forename: J G
authors.initials: JG
authors.lastname: Aparicio
authors.forename: S H
authors.initials: SH
authors.lastname: Nam
authors.forename: M L
authors.initials: ML
authors.lastname: Applebury
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002417:Cattle; D018076:DNA, Complementary; D006162:Guanylate Cyclase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008168:Lung / Q000201:enzymology; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D010902:Pituitary Gland / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats; D011956:Receptors, Cell Surface; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled; D018000:Receptors, Peptide / Q000235:genetics*; D012160:Retina / Q000201:enzymology; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; D006162:Guanylate Cyclase; C484781:Gucy2e protein, rat; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled