Centrifugation does not alter spatial distribution of 'BEP4' mRNA in paracentrotus lividus EGG.
Paracentrotus lividus unfertilized eggs were centrifuged in a sucrose gradient, so to split each into two parts: a nucleated light fragment and an anucleated heavy fragment. Northern blot analyses utilizing a bep4 probe as animal marker and H2A histone gene and 12S-mit RNA as controls indicate that the eggs are elongated along the animal-vegetal axis during centrifugation and thereafter split into an animal and a vegetal half. Treatment of the eggs with colchicine before centrifugation abolishes the animal localization of bep4 mRNA.
Costa
C
C
Istituto di Biologia dello Sviluppo C.N.R., Palermo, Italy.
Rinaldi
A M
AM
Romancino
D P
DP
Cavalcante
C
C
Vizzini
A
A
Di Carlo
M
M
D000818:Animals; D015152:Blotting, Northern; D002458:Cell Fractionation; D016764:Cell Polarity; D002499:Centrifugation, Density Gradient; D003078:Colchicine / Q000494:pharmacology; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules; D010063:Ovum / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012617:Sea Urchins
C065555:Bep4 protein, Paracentrotus lividus; D008565:Membrane Proteins; D012333:RNA, Messenger; D003078:Colchicine