title: Isolation and characterization of an amino acid-selective channel protein present in the chloroplastic outer envelope membrane.
abstract: The reconstituted pea chloroplastic outer envelope protein of 16 kDa (OEP16) forms a slightly cation-selective, high-conductance channel with a conductance of Lambda = 1,2 nS (in 1 M KCl). The open probability of OEP16 channel is highest at 0 mV (Popen = 0.8), decreasing exponentially with higher potentials. Transport studies using reconstituted recombinant OEP16 protein show that the OEP16 channel is selective for amino acids but excludes triosephosphates or uncharged sugars. Crosslinking indicates that OEP16 forms a homodimer in the membrane. According to its primary sequence and predicted secondary structure, OEP16 shows neither sequence nor structural homologies to classical porins. The results indicate that the intermembrane space between the two envelope membranes might not be as freely accessible as previously thought.
authors.affiliation: Botanisches Institut, Universität Kiel, D-24098 Kiel, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Pohlmeyer
authors.forename: J
authors.initials: J
authors.lastname: Soll
authors.forename: T
authors.initials: T
authors.lastname: Steinkamp
authors.forename: S
authors.initials: S
authors.lastname: Hinnah
authors.forename: R
authors.initials: R
authors.lastname: Wagner
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D060365:Chloroplast Proteins; D002736:Chloroplasts / Q000378:metabolism*; D007473:Ion Channels / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010944:Plants / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000596:Amino Acids; D060365:Chloroplast Proteins; D007473:Ion Channels; D010940:Plant Proteins