DNA-based vaccination using Schistosoma japonicum (Asian blood-fluke) genes.
We have examined the efficacy of nucleic acid vaccination in inducing immunity to the multicellular parasite, Schistosoma japonicum, a trematode worm responsible for causing schistosomiasis in humans and other mammalian species. A panel of Schistosoma japonicum cDNAs were cloned into eukaryotic expression vectors, injected into animals, and tested for immunogenicity. The cDNAs tested encoded 26- and 28-kDa glutathione-S-transferases, calreticulin, glyceraldehyde-3-phosphate dehydrogenase, a 22.6 kDa membrane-associated antigen, a 14 kDa fatty-acid binding protein, fragments of paramyosin, full-length paramyosin, and a novel gene comprising the 26 kDa glutathione-S-transferase fused to a fragment of paramyosin cDNA. The paramyosin gene constructs, including the fusion, were all able to induce anti-paramyosin antibodies; with the fragments of paramyosin these were of the IgG1, IgG2a and IgG2b isotypes. In contrast, none of the other schistosome cDNAs tested were able to induce detectable antibody responses. The anti-paramyosin antibodies did not protect mice challenged with cercariae of S. japonicum.
Waine
G J
GJ
Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, Royal Brisbane Hospital, Herston, Australia.
Yang
W
W
Scott
J C
JC
McManus
D P
DP
Kalinna
B H
BH
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000276:immunology; D037282:Calreticulin; D005260:Female; D017238:Genes, Helminth; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000276:immunology; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D011817:Rabbits; D051381:Rats; D011916:Rats, Inbred F344; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012261:Ribonucleoproteins / Q000235:genetics / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology / Q000517:prevention & control*; D014335:Tropomyosin / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology
D000909:Antibodies, Helminth; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D014335:Tropomyosin; D019444:Vaccines, DNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005982:Glutathione Transferase