title: Survival of mycoplasmas inoculated in horse sera.
abstract: Although it is known that commercialized bovine serum is sometimes contaminated with mycoplasmas, it is not clear whether mycoplasmas can survive in horse serum. In this study, as a preliminary examination of the survival of mycoplasmas inoculated in horse sera, the survivability of 8 strains of 7 mycoplasmas was tested. The results obtained reveal that two strains of M. bovis and M. gallisepticum were found to survive in non-heated and inactivated sera for 94 to 330 days at 30 or 37 degrees C. Three strains of M. bovirhinis, M. gateae and A. laidlawii lived for 7 to 330 days depending upon the temperature maintained or pH of the serum. Strains of M. bovigenitalium and U. diversum survived for a maximum of 8 days in all horse sera tested. Therefore, mycoplasmas are generally likely to survive for a long period in horse serum although the survival period depends on the species, strain and temperature.
authors.affiliation: Division of Veterinary Science, Faculty of Agriculture, Miyazaki University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagatomo
authors.forename: Y
authors.initials: Y
authors.lastname: Tokita
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D006736:Horses / Q000097:blood*; D007106:Immune Sera; D009174:Mycoplasma / Q000302:isolation & purification / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
chemical_list: D007106:Immune Sera