title: Analysis of specific accumulation of radiolabeled anti-carcinoembryonic antigen monoclonal antibody CEA 102 in colorectal cancer using computed radiography.
abstract: The specific accumulation of radiolabeled anti-carcinoembryonic antigen (CEA) monoclonal antibody (MoAb) CEA 102 in colorectal cancers was investigated by autoradiography of surgical specimens using Fuji Computed Radiography (FCR). Five patients with colorectal cancer were injected intravenously with [13I]-labeled intact CEA 102 or its F(ab')2. Primary tumor and liver metastases were successfully detected by external scanning with gamma camera in four cases. Autoradiographic study using FCR showed predominant localization of [13I]-labeled CEA 102 in primary tumors and liver metastases in all cases. Even the small-sized liver metastasis (0.5 cm) was clearly visualized. The pixel distribution curves showed the heterogeneity of the distribution of administered MoAb in the tumors. In the quantitative distribution analysis of CEA 102, the uptake of the primary tumor was 10-fold greater than that of the normal colon mucosa. These results suggest that MoAb has great potential in radioimmunodetection as well as possible use in antibody-directed therapy.
authors.affiliation: Fujita Health University Nanakuri Sanatorium, Mie, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: M
authors.initials: M
authors.lastname: Tadokoro
authors.forename: J
authors.initials: J
authors.lastname: Sakamoto
authors.forename: H
authors.initials: H
authors.lastname: Murayama
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Sakuma
authors.forename: H
authors.initials: H
authors.lastname: Takagi
mesh_terms: D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D000912:Antibodies, Neoplasm; D001345:Autoradiography; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000276:immunology; D011877:Radionuclide Imaging; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D002272:Carcinoembryonic Antigen