B lymphocytes of xeroderma pigmentosum or Cockayne syndrome patients with inherited defects in nucleotide excision repair are fully capable of somatic hypermutation of immunoglobulin genes.
Recent experiments have strongly suggested that the process of somatic mutation is linked to transcription initiation. It was postulated that a mutator factor loads onto the RNA polymerase and, during elongation, causes transcriptional arrest that activates DNA repair, thus occasionally causing errors in the DNA sequence. We report the analysis of the role of one of the known DNA repair systems, nucleotide excision repair (NER), in somatic mutation. Epstein-Barrvirus-transformed B cells from patients with defects in NER (XP-B, XP-D, XP-V, and CS-A) were studied. Their heavy and light chain genes show a high frequency of point mutations in the variable (V), but not in the constant (C) regions. This suggests that these B cells can undergo somatic hypermutation despite significant defects in NER. Thus, it is doubtful that NER is an essential part of the mechanism of somatic hypermutation of Ig genes. As an aside, NER seems also not involved in Ig gene switch recombination.
Kim
N
N
Department of Biochemistry, University of Chicago, Illinois 60637, USA.
Kage
K
K
Matsuda
F
F
Lefranc
M P
MP
Storb
U
U
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism*; D002461:Cell Line, Transformed; D002648:Child; D002675:Child, Preschool; D002999:Clone Cells; D003001:Cloning, Molecular; D003057:Cockayne Syndrome / Q000235:genetics* / Q000276:immunology*; D004252:DNA Mutational Analysis; D004260:DNA Repair / Q000276:immunology*; D005260:Female; D005803:Genes, Immunoglobulin / Q000276:immunology*; D004854:Herpesvirus 4, Human; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008297:Male; D017354:Point Mutation / Q000276:immunology*; D014983:Xeroderma Pigmentosum / Q000235:genetics* / Q000276:immunology*
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region