Determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in human urine.
A method for simultaneous determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in urine is described. These compounds are metabolites of N-methyl-2-pyrrolidone, a powerful and widely used organic solvent. 5-Hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide were purified from urine by adsorption to a C8 solid-phase extraction column and then elution by ethyl acetate-methanol (80:20). After evaporation, the samples were derivatised at 100 degrees C for 1 h by bis(trimethylsilyl)trifluoroacetamide. Ethyl acetate was then added and the samples were analysed by gas chromatography-mass spectrometry in the electron impact mode. The extraction recovery for 5-hydroxy-N-methylpyrrolidone was about 80% while that for 2-hydroxy-N-methylsuccinimide was about 30%. The intra-day precision for 5-hydroxy-N-methylpyrrolidone was 2-4% and the between-day precision 4-21% (4 and 60 microg/ml). The intra-day precision for 2-hydroxy-N-methylsuccinimide was 4-8% and the between-day precision 6-7% (2 and 20 microg/ml). The detection limit was 0.2 microg/ml urine for both compounds. The method is applicable for analysis of urine samples from workers exposed to N-methyl-2-pyrrolidone.
Jönsson
B A
BA
Department of Occupational and Environmental Medicine, University Hospital, Lund, Sweden.
Akesson
B
B
D000395:Air Pollutants, Occupational / Q000378:metabolism / Q000633:toxicity; D004784:Environmental Monitoring; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011760:Pyrrolidinones / Q000378:metabolism* / Q000633:toxicity / Q000652:urine*; D012680:Sensitivity and Specificity; D013388:Succinimides / Q000652:urine*
C107901:2-hydroxy-N-methylsuccinimide; C107902:5-hydroxy-N-methylpyrrolidone; D000395:Air Pollutants, Occupational; D011760:Pyrrolidinones; D013388:Succinimides; C038678:N-methylpyrrolidone