title: Bicarbonate loading in the thoroughbred: dose, method of administration and acid-base changes.
abstract: Two studies were undertaken in an attempt to investigate the optimal dose, route and time course required to 'bicarbonate load' the Thoroughbred. During the first study a test solution of sodium bicarbonate (NaHCO3) and a control solution of water were administered via a nasogastric tube to six horses. Blood acid-base status was determined immediately prior to and for 24 h following intubation. During the second study a standardised feed was given to four horses, with and without NaHCO3 supplementation. This was followed by a further trial involving the addition of glucose and NaHCO3 to a standardised feed. During Study 1, plasma pH peaked 8 h after NaHCO3 intubation (7.433 +/- 0.020) and levels were still elevated above control values 12 h post intubation. Although there was a trend for blood PCO2 to be elevated above control values at all times after NaHCO3 administration, values were only significantly different at 4 and 5 h post intubation. The patterns of change in plasma HCO3- and blood base-excess after NaHCO3 administration were similar. Values peaked between 3 and 6 h post intubation and were still above control values 12 h post intubation. In Study 2 attempts to alter acid-base status by presenting NaHCO3 mixed with feed were unsuccessful because horses failed to consume the total mix, despite attempts to mask the unpalatable nature of the feed by adding glucose. The results suggest that future studies investigating the influence of NaHCO3 on exercise performance and metabolism should possibly involve administration of NaHCO3 in a greater quantity than is administered conventionally (0.3 g/kg). More meaningful results may be obtained if NaHCO3 is administered as a solution, via a nasogastric tube, at least 3 h prior to exercise.
authors.affiliation: Department Comparative Physiology, Animal Health Trust, Newmarket, Suffolk.
authors.forename: P L
authors.initials: PL
authors.lastname: Greenhaff
authors.forename: D H
authors.initials: DH
authors.lastname: Snow
authors.forename: R C
authors.initials: RC
authors.lastname: Harris
authors.forename: C A
authors.initials: CA
authors.lastname: Roberts
mesh_terms: D000136:Acid-Base Equilibrium / Q000187:drug effects* / Q000502:physiology; D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D001639:Bicarbonates / Q000008:administration & dosage* / Q000097:blood / Q000494:pharmacology*; D001786:Blood Glucose / Q000032:analysis; D002245:Carbon Dioxide / Q000097:blood; D004032:Diet / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D004750:Enteral Nutrition / Q000379:methods / Q000662:veterinary; D005260:Female; D005947:Glucose / Q000008:administration & dosage; D006736:Horses / Q000097:blood / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology; D013997:Time Factors
chemical_list: D001639:Bicarbonates; D001786:Blood Glucose; D002245:Carbon Dioxide; D005947:Glucose