Attenuated bacteria as a DNA delivery vehicle for DNA-mediated immunization.
Previously, Shigella carrier 15D was shown to deliver a mammalian DNA expression plasmid to cultured cells with subsequent production of the plasmid-encoded foreign protein. In this study, we report in vivo delivery of a DNA expression plasmid to mucosal tissue results in the stimulation of immune responses against the plasmid-encoded foreign antigen. Splenocytes from mice receiving two intranasal inoculations of 15D carrying pCMV beta showed proliferative responses to the plasmid-encoded Escherichia coli beta-galactosidase. In addition, antibody specific for beta-galactosidase was detected in pooled sera collected from 15D (pCMV beta) infected mice.
Sizemore
D R
DR
Megan Health Inc. St Louis, MO 63110, USA.
Branstrom
A A
AA
Sadoff
J C
JC
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002478:Cells, Cultured; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000276:immunology; D004279:DNA, Viral / Q000276:immunology; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000201:enzymology; D005260:Female; D005434:Flow Cytometry; D018928:Immunity, Mucosal; D051379:Mice; D008807:Mice, Inbred BALB C; D014677:Pharmaceutical Vehicles; D010957:Plasmids / Q000235:genetics; D012760:Shigella / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000008:administration & dosage*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology; D001616:beta-Galactosidase / Q000235:genetics
D000914:Antibodies, Viral; D004279:DNA, Viral; D014677:Pharmaceutical Vehicles; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014765:Viral Vaccines; D001616:beta-Galactosidase