Detection of rifampin resistance in Mycobacterium tuberculosis by use of a rapid, simple, and specific RNA/RNA mismatch assay.
An adaption of an RNA/RNA duplex, base pair-mismatch assay is capable of detecting rifampin resistance in Mycobacterium tuberculosis. The specificity and sensitivity of the mismatch assay in detecting rifampin resistance were 100% and 96%, respectively, when tested against 46 rifampin-resistant and rifampin-susceptible strains of M. tuberculosis. By use of a range of mycobacterial and nonmycobacterial prokaryote pathogens, the mismatch assay was shown to be specific for M. tuberculosis and Mycobacterium bovis. The assay is cost-effective compared with DNA sequencing and other molecular methods and is simple to perform and interpret. Furthermore, the assay can return a result within 24 h after receipt of an isolated organism and potentially can be used directly with smear-positive specimens.
Nash
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KA
Children's Hospital Los Angeles, University of Southern California, 90027, USA.
Gaytan
A
A
Inderlied
C B
CB
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D012329:RNA, Bacterial / Q000032:analysis*; D012260:Ribonucleases; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity
D000904:Antibiotics, Antitubercular; D004269:DNA, Bacterial; D009692:Nucleic Acid Heteroduplexes; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases; D012293:Rifampin