PolI-driven integrative expression vectors for yeast.
A novel expression vector for yeast has been constructed from the regulatory elements present in the polI promoter and the enhancer/termination region (E/T) of rDNA. Under some conditions, this promoter/vector combination produces small RNAs such as the hammerhead RNA sequence at levels comparable to polII- and polIII-dependent systems. No stable transcription product can be demonstrated with this vector when the enhancer/termination sequence is less than 100 nucleotides downstream from the promoter. On the other hand, high expression of a stable, hammerhead RNA molecule can be obtained from this vector by inserting a 400-bp fragment containing the ADH1 transcription termination region upstream of the E/T. RNAs produced by this vector are polyadenylated and multiple copies of this plasmid can be stably integrated into the yeast chromosome.
Blancafort
P
P
Département de Biochimie, Université de Montréal, Québec, Canada.
Ferbeyre
G
G
Sariol
C
C
Cedergren
R
R
D005822:Genetic Vectors; D012318:RNA Polymerase I / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D015003:Yeasts / Q000235:genetics*
D012333:RNA, Messenger; D012318:RNA Polymerase I