A novel method for the non-chromatographic purification of technetium-99m-labeled monoclonal antibodies: a study with B72.3 monoclonal antibody.
A cleaning resin has been developed for the non-chromatographic purification of 99mTc-labeled monoclonal antibodies (MAbs). The resin used is a modified form of thiopropylsepharose 6B resin, in which its sulfhydryl groups have been tinylated with stannous chloride. The method requires only simple stirring of the radiolabeling reaction mixture with this tinylated resin and subsequent separation of it from the resin by filtration to obtain a 99mTc-labeled MAb of radiopharmaceutical purity. The method provides an alternative to chromatographic purification of the radiolabeled MAb (i.e. gel filtration or anion exchange chromatography) which has been used in other 99mTc-MAb preparations. For comparison studies, we labeled the B72.3 MAb with NeoRx's diamide dimercaptide chelate radiolabeling kit, split the reaction mixture into two equal portions and then purified one portion with anion exchange chromatography (NeoRx's chosen method) while the other portion was purified with our cleaning resin. Comparison of HPLC chromatograms, percent 99mTc-bound to MAb, biodistribution and scintigraphic results show that our cleaning resin methodology provides a 99mTc-labeled MAb of essentially equal purity and utility as does the established, chromatographic one.
Rosenzweig
H S
HS
Department of Radiation Oncology, University of Pittsburgh, PA 15213, USA.
Ranadive
G N
GN
Seskey
T
T
Epperly
M W
MW
Bloomer
W D
WD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D015394:Molecular Structure; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000302:isolation & purification*; D012116:Resins, Plant; D012685:Sepharose / Q000031:analogs & derivatives*; D013667:Technetium / Q000302:isolation & purification; D014018:Tissue Distribution / Q000502:physiology; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D019275:Radiopharmaceuticals; D012116:Resins, Plant; C035872:thiopropyl-sepharose; D013667:Technetium; D012685:Sepharose