ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor.
We describe the purification and characterization of ACF, an ATP-utilizing chromatin assembly and remodeling factor. ACF is a multisubunit factor that contains ISWI protein and is distinct from NURF, another ISWI-containing factor. In chromatin assembly, purified ACF and a core histone chaperone (such as NAP-1 or CAF-1) are sufficient for the ATP-dependent formation of periodic nucleosome arrays. In chromatin remodeling, ACF is able to modulate the internucleosomal spacing of chromatin by an ATP-dependent mechanism. Moreover, ACF can mediate promoter-specific nucleosome reconfiguration by Gal4-VP16 in an ATP-dependent manner. These results suggest that ACF acts catalytically both in chromatin assembly and in the remodeling of nucleosomes that occurs during transcriptional activation.
Ito
T
T
Department of Biology, University of California, San Diego, La Jolla 92093-0347, USA.
Bulger
M
M
Pazin
M J
MJ
Kobayashi
R
R
Kadonaga
J T
JT
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002843:Chromatin / Q000502:physiology*; D004330:Drosophila / Q000196:embryology / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000502:physiology; D046911:Macromolecular Substances; D009707:Nucleosomes / Q000502:physiology; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic
D002843:Chromatin; C086468:ISWI protein; D046911:Macromolecular Substances; D009707:Nucleosomes; D014157:Transcription Factors; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases