title: The significance of Ras guanine nucleotide exchange factor, son of sevenless protein, in renal cell carcinoma cell lines.
abstract: OBJECTIVE
  The aim of the present study is to clarify the significance of the Ras guanine-nucleotide exchange reaction in the proliferation of human renal cell carcinoma cell lines.
  
  
  METHODS
  We examined the expression of human son of sevenless-1 (hSos-1) protein and the epidermal growth factor (EGF) receptor in human renal cell carcinoma cell lines by Western blot analysis. Additionally, a dominant negative H-ras mutant, N116Y, which is known to inhibit the Ras guanine-nucleotide exchange reaction, was transfected into these cell lines by lipofection.
  
  
  RESULTS
  Human renal cell carcinoma cell lines expressed much higher amounts of the EGF receptor and hSos-1 protein than normal kidney tissue. Moreover, the N116Y ras mutant could strongly suppress cellular proliferation in these cell lines.
  
  
  CONCLUSIONS
  Augmentation of the Ras guanine-nucleotide exchange reaction might be essential to the proliferation of human renal cell carcinoma cells.
authors.affiliation: Department of Urology, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shinohara
authors.forename: Y
authors.initials: Y
authors.lastname: Ogiso
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Sazawa
authors.forename: T
authors.initials: T
authors.lastname: Harabayashi
authors.forename: T
authors.initials: T
authors.lastname: Koyanagi
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D066246:ErbB Receptors / Q000235:genetics*; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras / Q000235:genetics; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D011506:Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured; D020663:ras Guanine Nucleotide Exchange Factors
chemical_list: D015852:Eukaryotic Initiation Factor-2; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D020663:ras Guanine Nucleotide Exchange Factors; D066246:ErbB Receptors