Direct injection analysis of chlorzoxazone and its major metabolite 6-hydroxychlorzoxazone in human serum using a semipermeable surface (SPS) HPLC column.
A semipermeable surface (SPS) HPLC column has been used to develop a direct injection method for the simultaneous determination of chlorzoxazone and its 6-hydroxy metabolite in serum. Spiked serum was filtered and 20 microL injected directly onto the SPS column. The separation was achieved within 25 min using a mobile phase of 20:80 v/v acetonitrile-aqueous 0.05 M phosphate buffer pH 7 with detection at 230 nm. The method was linear in the concentration range 0.5-20 micrograms/mL (r > 0.999, n = 6) with an RSD < 5%. The limits of quantitation and detection were 0.5 microgram/mL and 0.1 microgram/mL (S/N > 3), respectively, for both drug and metabolite. Intraday and interday reproducibility was < or = 6% with recoveries of drug and metabolite > 94%.
Haque
A
A
Department of Medicinal Chemistry, College of Pharmacy, University of Georgia, Athens 30602-2352, USA.
Stewart
J T
JT
D002753:Chlorzoxazone / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D006801:Humans; D009125:Muscle Relaxants, Central / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results; D013499:Surface Properties
D009125:Muscle Relaxants, Central; D002753:Chlorzoxazone