title: Evaluation of argyrophilic nucleolar organizer regions (AgNORs) in oral carcinomas in relation to human papillomavirus infection and cytokinetics.
abstract: The numbers of argyrophilic nucleolar organizer regions (AgNORs) were quantified in oral carcinomas (n = 39) with or without human papillomavirus (HPV) infection. The AgNOR counts of the HPV-positive samples (7.15 +/- 2.13) were not significantly (P = 0.09) higher than those of the HPV-negative ones (6.16 +/- 1.89). Furthermore, the lesions infected with multiple HPV types had greater counts than those with HPV type 16/18 infection alone. Significant differences were observed between the mean counts of the poorly (10.50 +/- 0.54), moderately (7.31 +/- 1.07) and well- (5.12 +/- 0.85) differentiated carcinomas. The mean AgNOR numbers in the oral carcinomas at TNM stages III/IV were found to be significantly (P < 0.01) higher than the numbers in corresponding stage II lesions. Cytokinetics of the lesions assessed by the bromodeoxyuridine (Brdu) labelling index (LI%) showed a linear correlation (r = 0.91; P < 0.0001) with their respective mean AgNOR counts.
authors.affiliation: Department of Tumor Virology, Chittaranjan National Cancer Institute, Calcutta, India.
authors.forename: R
authors.initials: R
authors.lastname: Chatterjee
authors.forename: D
authors.initials: D
authors.lastname: Mukhopadhyay
authors.forename: R N
authors.initials: RN
authors.lastname: Chakraborty
authors.forename: R B
authors.initials: RB
authors.lastname: Mitra
mesh_terms: D000328:Adult; D000368:Aged; D001973:Bromodeoxyuridine / Q000032:analysis; D002455:Cell Division; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000150:complications* / Q000473:pathology / Q000648:ultrastructure*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications*; D011379:Prognosis; D016622:Silver Staining; D014412:Tumor Virus Infections / Q000150:complications
chemical_list: D001973:Bromodeoxyuridine