title: Isolation of a new Orientia tsutsugamushi serotype.
abstract: Orientia tsutsugamushi, the etiological agent of scrub typhus, is an antigenically diverse organism and many serologically distinct strains have been identified. The 56 kDa protein of O. tsutsugamushi, a major protein in the outer membrane, has been thought to be responsible for this antigenic variability. A strain of O. tsutsugamushi isolated in Korea cross-reacted with both Gilliam strain-specific and Karp strain-specific monoclonal antibodies. When its 56 kDa protein gene was cloned and analyzed, its sequence showed variation especially between 1,200 and 1,250 bp, showing that this isolate is a new O. tsutsugamushi strain.
authors.affiliation: Department of Microbiology, Seoul National University College of Medicine, Republic of Korea.
authors.forename: S Y
authors.initials: SY
authors.lastname: Seong
authors.forename: S G
authors.initials: SG
authors.lastname: Park
authors.forename: H R
authors.initials: HR
authors.lastname: Kim
authors.forename: T H
authors.initials: TH
authors.lastname: Han
authors.forename: J S
authors.initials: JS
authors.lastname: Kang
authors.forename: M S
authors.initials: MS
authors.lastname: Choi
authors.forename: I S
authors.initials: IS
authors.lastname: Kim
authors.forename: W H
authors.initials: WH
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D005798:Genes, Bacterial; D006801:Humans; D007723:Korea; D008969:Molecular Sequence Data; D012285:Orientia tsutsugamushi / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D012612:Scrub Typhus / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins