Quenching of nitric oxide by an oral carbonaceous adsorbent.
The ability of carbonaceous particles (AST-120), originally developed as an enteral adsorbent of uremic toxins, to quench nitric oxide (NO) was tested. NO in solutions prepared by two methods [NO gas bubbling and NO generating system, i.e., decomposition of 1-hydroxy-2-oxo-3-(aminopropyl)-3-isopropyl-1-triazene] were determined by a NO-specific reduction of carboxy-2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide using an electron paramagnetic resonance spectrometry. NO concentrations were less in samples containing increasing concentrations of AST-120. In a separate study, nitrite concentrations in lipopolysaccharide-treated RAW264 cells were significantly less in incubation medium containing AST-120. Thus, AST-120 may be applicable as an enteral anti-NO agent.
Iwamoto
N
N
Department of Pediatric Nephrology, Tokyo Women's Medical College, Japan.
Ito
K
K
Kakuchi
J
J
Yoshioka
T
T
D000327:Adsorption; D002244:Carbon / Q000494:pharmacology*; D002460:Cell Line; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism; D009569:Nitric Oxide / Q000737:chemistry / Q000378:metabolism*; D010087:Oxides / Q000494:pharmacology*
D010087:Oxides; D009569:Nitric Oxide; D002244:Carbon; C040896:AST 120