Direct action of nitric oxide on osteoblastic differentiation.
The effect of nitric oxide (NO) on osteoblastic differentiation was examined in cultured mouse osteoblasts. Interleukin-1beta and tumor necrosis factor-alpha expressed inducible NO synthase gene with little effect on constitutive NO synthase gene. These cytokines increased NO production, which was inhibited by L-NMMA pretreatment, and decreased alkaline phosphatase (AIPase) activity, which was not restored by L-NMMA. Furthermore, NO donors, sodium nitroprusside and NONOate dose-dependently elevated AIPase activity and expression of osteocalcin gene. These results suggest that NO directly facilitates osteoblastic differentiation and the cytokine-induced inhibition of AIPase activity is mediated via mechanism other than NO.
Hikiji
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H
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Tokyo, Japan. hhikiji-tky@umin.u-tokyo.ac.jp
Shin
W S
WS
Oida
S
S
Takato
T
T
Koizumi
T
T
Toyo-oka
T
T
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D006834:Hydrazines / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000235:genetics; D009589:Nitrogen Oxides; D009599:Nitroprusside / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D015675:Osteocalcin / Q000235:genetics; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D006834:Hydrazines; D007375:Interleukin-1; D009589:Nitrogen Oxides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D015675:Osteocalcin; D009599:Nitroprusside; D009569:Nitric Oxide; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D019001:Nitric Oxide Synthase; D000469:Alkaline Phosphatase; D006152:Cyclic GMP; D015232:Dinoprostone