A rapid viability assay for Cryptosporidium oocysts and Giardia cysts for use in conjunction with indirect fluorescent antibody detection.
There is an urgent need to develop rapid methods to determine the viability of Cryptosporidium oocysts and Giardia cysts in environmental samples, especially water. The inclusion of the vital dye propidium iodide (PI) by oocysts and cysts has been previously shown to correlate well with nonviability. The ability of nonviable oocysts and cysts to include PI has been employed to develop a rapid viability determination method that could be used in conjunction with the current indirect fluorescent antibody (IFA) method for detecting oocysts and cysts. The efficacy of this PI-IFA method to detect and determine the viability status of oocysts/cysts has been tested using oocyst samples inactivated by three different approaches. The ability to incorporate PI staining with IFA detection provides the advantage of both detection and viability determination at the same time, using the same sample.
Dowd
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SE
Environmental Science Program, Texas A&M University Research Center, El Paso 79927, USA.
Pillai
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SD
D000818:Animals; D002470:Cell Survival; D003458:Cryptosporidium / Q000166:cytology / Q000302:isolation & purification*; D019084:Fluorescent Antibody Technique, Indirect; D005872:Giardia / Q000166:cytology / Q000302:isolation & purification*; D011419:Propidium; D011634:Public Health; D013194:Staining and Labeling / Q000379:methods*; D014867:Water / Q000469:parasitology*; D014881:Water Supply
D014867:Water; D011419:Propidium