Reconstitution of B cell subsets in Rag deficient mice by transplantation of in vitro differentiated embryonic stem cells.
In vitro differentiated embryonic stem (ES) cells contain a population which is similar to fetal liver pro/pre-B cells on the basis of cell surface antigens and cytoplasmic expression of immunoglobin heavy chain. This population was purified and transplanted into Rag-1 deficient recipients to characterize its developmental potential in vivo. Following intravenous transfer, these cells rapidly reconstituted the splenic B but not the T cell compartment. Reconstitution was transient, indicating the lack of long-term reconstituting capacity. Similar to fetal liver, B-1 type as well as conventional B cells were generated, accompanied by high serum IgM levels. Intraperitoneal injection generated high numbers of peritoneal B cells, predominately of the B-1a phenotype, with poor splenic repopulation and low serum IgM levels. These observations suggest the emergence of two different B lineage precursor populations during in vitro ES cell differentiation and define a possible role of the microenvironment in directing lymphoid development.
Potocnik
A J
AJ
Max-Planck-Institute for Immunobiology, Freiburg, Germany. potocnik@immunbio.mpg.de
Nerz
G
G
Kohler
H
H
Eichmann
K
K
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D018956:CD5 Antigens / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D016654:Genes, RAG-1; D018380:Hematopoietic Stem Cell Transplantation; D018398:Homeodomain Proteins; D007072:Immunoglobulin D / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000166:cytology
D018956:CD5 Antigens; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D007072:Immunoglobulin D; D007075:Immunoglobulin M; C064658:RAG-1 protein; D017493:Leukocyte Common Antigens