Soluble highly coloured phenylboronic acids and their use in glycohemoglobin quantification.
Water soluble dye-phenylboronic acid conjugates (dye-PBAs) possessing strong absorption of visible light are introduced as new reagents for the determination of glycohemoglobin. Their functionality and prospective use are demonstrated in a semi-homogenous glycohemoglobin assay. The assay is based on cis-diol esterification of dye-PBA to glycohemoglobin followed by selective precipitation of hemoglobin from solution, co-precipitating bound dye-PBA. Quantification of the molar "dye-PBA/Hb"-ratio in redissolved precipitates using either absorption or fluorescence spectroscopy, reflects the glycation level of the blood samples used. Future development of the assay principle is illustrated in a filter based assay, collecting the precipitated hemoglobin on a filter followed by reflectometric readings directly on the precipitate. The significance of this work lies first, in the demonstration of a new principle for the determination of glycohemoglobin, and second, as an illustration of the prospective use of water soluble, signal-forming non-immobilised boronic acids in the determination of cis-diol containing analytes.
Frantzen
F
F
Axis Nord, Mørkved, Norway.
Grimsrud
K
K
Heggli
D E
DE
Sundrehagen
E
E
D001774:Blood Chemical Analysis / Q000379:methods*; D001897:Boronic Acids; D004396:Coloring Agents; D005069:Evaluation Studies as Topic; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D010078:Oxazines; D011166:Porphyrins; D012995:Solubility; D013050:Spectrometry, Fluorescence
D001897:Boronic Acids; D004396:Coloring Agents; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006442:Glycated Hemoglobin; D007202:Indicators and Reagents; D010078:Oxazines; D011166:Porphyrins; C018395:tetracarboxyphenylporphine; C014180:resorufin; C010686:benzeneboronic acid