title: Triglyceride hydrolysis of soy oil vs fish oil emulsions.
abstract: BACKGROUND
  Fish oil triglycerides (TG) are being considered for use in IV lipid emulsions, but the characteristics of their lipase-mediated clearance from plasma are largely unknown.
  
  
  METHODS
  We compared the in vitro hydrolysis of soy oil long-chain triglyceride emulsions (LCT) and fish oil emulsions (omega-3) using lipoprotein (LPL) and hepatic (HL) lipases, omega-3 emulsions contained 18% and 28% of total TG fatty acid as eicosapentaenoic acid (EPA) and docosahexanoic acid (DHA), respectively.
  
  
  RESULTS
  Under conditions of maximal hydrolysis, total free fatty acid (FFA) release was two- to threefold greater with LCT compared with omega-3 emulsions. Also, EPA and DHA together contributed proportionally much less than other fatty acids (< 20%) to FFA released from omega-3 emulsions. In mixtures of LCT emulsion with omega-3 emulsions, the presence of > 20% of omega-3 particles substantially inhibited LCT emulsion hydrolysis (by up to 50%).
  
  
  CONCLUSIONS
  Our results suggest that, during infusion of omega-3 emulsions, EPA and DHA may enter cells as TG or partial glycerides within emulsion particles and not as FFA and that coinfusion of omega-3 emulsion with LCT emulsion at low omega-3:LCT emulsion ratios (up to 20% of total triglyceride as omega-3) will not substantially inhibit LCT hydrolysis.
authors.affiliation: Department of Pediatrics, Columbia University, New York, NY 10032, USA.
authors.forename: F L
authors.initials: FL
authors.lastname: Oliveira
authors.forename: S C
authors.initials: SC
authors.lastname: Rumsey
authors.forename: E
authors.initials: E
authors.lastname: Schlotzer
authors.forename: I
authors.initials: I
authors.lastname: Hansen
authors.forename: Y A
authors.initials: YA
authors.lastname: Carpentier
authors.forename: R J
authors.initials: RJ
authors.lastname: Deckelbaum
mesh_terms: D004281:Docosahexaenoic Acids / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D005217:Fat Emulsions, Intravenous / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D005395:Fish Oils / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D013024:Soybean Oil / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D005217:Fat Emulsions, Intravenous; D005230:Fatty Acids, Nonesterified; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D014280:Triglycerides; D004281:Docosahexaenoic Acids; D013024:Soybean Oil; D015118:Eicosapentaenoic Acid; D008049:Lipase; D008071:Lipoprotein Lipase