title: Magnetic iron oxide particles coated with carboxydextran for parenteral administration and liver contrasting. Pre-clinical profile of SH U555A.
abstract: OBJECTIVE
  To evaluate the physical and pharmacological profiles of SH U555A, a suspension of magnetic iron oxide particles that is designed to enhance the visualization of liver tumors and metastases.
  
  
  METHODS
  Chemical and physical methods were used to characterize the size and structure of these magnetic iron oxide particles in aqueous solution. The biodistribution and pharmacokinetics of the particles were studied in mice, rats and dogs. The imaging efficacy of the particles was demonstrated by MR imaging in rat liver tumors
  
  
  RESULTS
  The SH U555A particles consist of low-molecular-weight carboxydextran-coated iron oxides predominantly of the gamma-Fe2O3 form with a hydrodynamic diameter ranging from 57-59 nm and strong T2 relaxivity of 164 liters x mmol(-1) x s(-1) (water, 0.47 T). In rats the particles exhibited a dose-dependent half-life of between 2 and 3 days in the liver at a dose of 20 micromol Fe/kg and a shorter half-life at lower doses. No major side effects were found. In a rat tumor model the tumor-to-liver contrast was markedly improved after i.v. administration of SH U555A. At a dose of 14 micromol Fe/kg the half-maximal contrast-effect was obtained even in nonoptimized T1-weighted spin-echo images.
  
  
  CONCLUSIONS
  SH U555A is a superparamagnetic MR contrast agent for i.v. administration and has substantial potential for the demarcation of liver tumors.
authors.affiliation: Schering AG, Research Laboratories, Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Lawaczeck
authors.forename: H
authors.initials: H
authors.lastname: Bauer
authors.forename: T
authors.initials: T
authors.lastname: Frenzel
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Kito
authors.forename: N
authors.initials: N
authors.lastname: Miwa
authors.forename: H
authors.initials: H
authors.lastname: Tsutsui
authors.forename: H
authors.initials: H
authors.lastname: Vogler
authors.forename: H J
authors.initials: HJ
authors.lastname: Weinmann
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage / Q000493:pharmacokinetics; D003911:Dextrans; D004285:Dogs; D005260:Female; D052203:Ferrosoferric Oxide; D007501:Iron / Q000008:administration & dosage / Q000493:pharmacokinetics; D008099:Liver / Q000033:anatomy & histology*; D008114:Liver Neoplasms, Experimental / Q000175:diagnosis; D058185:Magnetite Nanoparticles; D008297:Male; D051379:Mice; D010087:Oxides / Q000008:administration & dosage / Q000493:pharmacokinetics; D011817:Rabbits; D051381:Rats; D013535:Suspensions; D014018:Tissue Distribution
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D013535:Suspensions; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide