title: Nonneutral evolution of tandem repeats in the mitochondrial DNA control region of lagomorphs.
abstract: The mitochondrial DNA of the European rabbit (Oryctolagus cuniculus) contains a tandem array of 153-bp repeats in the vicinity of the replication origin of the H-stand. Variation among molecules in the number of these repeats results in inter- and intraindividual length polymorphism (heteroplasmy). Generally, in an individual, one predominant molecular type is observed, the others representing a low percentage of the mtDNA content. At the tissue level, we observe a particular distribution of this polymorphism in the gonads compared with liver, kidneys, or brain, implying a relationship between the differentiation status of the cells and the types of new mtDNA molecules which appear and accumulate during lifetime. Similar tandem repeats were also found in the mtDNA noncoding region of European hares (Lepus europaeus), a cottontail (Sylvilagus floridanus), and a pika (Ochotona rufescens). The lengths and the sequences of these units evolve rapidly and in a concerted way, but the number of repeats is maintained in a narrow range, and an internal 20-bp segment is highly conserved. Constraints restrict the evolution of the primary sequence of these repeated units, the number of which is probably controlled by a stabilizing selection.
authors.affiliation: Centre de Genetique Moleculaire CNRS, France.
authors.forename: D
authors.initials: D
authors.lastname: Casane
authors.forename: N
authors.initials: N
authors.lastname: Dennebouy
authors.forename: H
authors.initials: H
authors.lastname: de Rochambeau
authors.forename: J C
authors.initials: JC
authors.lastname: Mounolou
authors.forename: M
authors.initials: M
authors.lastname: Monnerot
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009928:Organ Specificity; D011817:Rabbits / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial