Glutathione S-transferase-encoding gene as a potential probe for environmental bacterial isolates capable of degrading polycyclic aromatic hydrocarbons.
Homologs of the glutathione S-transferase (GST)-encoding gene were identified in a collection of aromatic hydrocarbon-degrading Sphingomonas spp. isolated from New Zealand, Antarctica, and the United States by using PCR primers designed from the GST-encoding gene of Sphingomonas paucimobilis EPA505. Sequence analysis of PCR fragments generated from these isolates and of the GST gene amplified from DNA extracted from polycyclic aromatic hydrocarbon (PAH)-contaminated soil revealed a high degree of conservation, which may make the GST-encoding gene a potentially useful marker for PAH-degrading bacteria.
Lloyd-Jones
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Manaaki Whenua-Landcare Research Ltd., Hamilton, New Zealand.
Lau
P C
PC
D000595:Amino Acid Sequence; D000864:Antarctic Regions; D015342:DNA Probes / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006088:Gram-Negative Aerobic Bacteria / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D009520:New Zealand; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D012988:Soil Microbiology; D014481:United States
D015342:DNA Probes; D011084:Polycyclic Aromatic Hydrocarbons; D005982:Glutathione Transferase