title: An analytical method for the quantitation of mannitol and disaccharides in serum: a potentially useful technique in measuring small intestinal permeability in vivo.
abstract: An electrochemical-HPLC method for the determination of mannitol and lactulose is presented which may facilitate routine testing of intestinal permeability by requiring only a single blood sample instead of a 6-hour urine collection. Chromatographic conditions are described which allow separation of the closely related sugars lactulose and the dietary disaccharides lactose and sucrose. Preliminary results in normal controls and patients with untreated coeliac disease are presented.
authors.affiliation: Gastroenterology Unit, City Hospital, Birmingham, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Cox
authors.forename: T H
authors.initials: TH
authors.lastname: Iqbal
authors.forename: B T
authors.initials: BT
authors.lastname: Cooper
authors.forename: K O
authors.initials: KO
authors.lastname: Lewis
mesh_terms: D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods*; D016022:Case-Control Studies; D002446:Celiac Disease / Q000097:blood / Q000503:physiopathology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D019422:Dietary Sucrose / Q000097:blood / Q000493:pharmacokinetics; D004187:Disaccharides / Q000097:blood*; D004563:Electrochemistry / Q000379:methods; D005260:Female; D006801:Humans; D007421:Intestine, Small / Q000502:physiology*; D007792:Lactulose / Q000097:blood; D008297:Male; D008353:Mannitol / Q000097:blood*; D008875:Middle Aged; D010539:Permeability
chemical_list: D019422:Dietary Sucrose; D004187:Disaccharides; D008353:Mannitol; D007792:Lactulose