Bromo-deoxyuridine based assay for detection of parasite and drug sensitivity in Plasmodium falciparum in vitro.
A non-radioactive, thymidine analogue-bromodeoxyuridine (Brdu), derivative of uridine has been used for incorporation in DNA in culture of P. falciparum at various dosages and at different time period. Parasite growth rate and effect of chloroquine in culture were monitored by microscopic observation of stained smears and incorporation of Brdu molecules were visualized by immunofluorescence and measured by enzyme immuno assay using anti-Brdu. Uptaking of Brdu in parasite is slower unlike tumour cells. A positive correlation between parasite growth and Brdu uptake measurement by ELISA has been observed.
Biswas
S
S
Malaria Research Centre, Marg, Delhi.
Valecha
N
N
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000378:metabolism*; D002738:Chloroquine / Q000494:pharmacology; D016054:DNA, Protozoan / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D010963:Plasmodium falciparum / Q000187:drug effects*
D000962:Antimalarials; D016054:DNA, Protozoan; D002738:Chloroquine; D001973:Bromodeoxyuridine