Differentiation of Campylobacter jejuni and Campylobacter coli by polymerase chain reaction.
A multiplex PCR assay was developed using two primer sets for the identification and differentiation of Campylobacter coli and Campylobacter jejuni. Primer Set I amplifies a 460-bp fragment present in C. coli and C. jejuni. Set II amplifies a 160-bp target unique to C. jejuni. When the assay was performed on reference strains, amplification of C. coli yielded only the 460-bp fragment. Amplification of C. jejuni generated both the 160- and 460-bp fragments. Campylobacter field strains (n = 85) isolated from raw poultry were identified by PCR and by conventional biochemical methods. Species determination by the two methods agreed for 83 of the 85 isolates examined. By PCR, 23 were identified as C. coli and 62 as C. jejuni. One isolate was unidentifiable by biochemical testing. The PCR assay identified this isolated as C. coli. In addition, one strain which was identified as C. coli by biochemical testing was determined to be C. jejuni by PCR. The PCR assay offers an alternative to traditional biochemical typing methods for the identification and differentiation of C. coli and C. jejuni isolated from poultry. It is accurate, simple to perform, and can be completed within 8 h.
Harmon
K M
KM
Enteric Diseases and Food Safety Research Unit, USDA, Ames, IA 50010, USA.
Ransom
G M
GM
Wesley
I V
IV
D000818:Animals; D017000:Campylobacter coli / Q000235:genetics* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D016133:Polymerase Chain Reaction; D011200:Poultry / Q000382:microbiology; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers