title: Rapid purification, partial characterization, and antimicrobial spectrum of the bacteriocin, Pediocin AcM, from Pediococcus acidilactici M.
abstract: The bacteriocin from Pediococcus acidilactici M, designated as Pediocin AcM, was rapidly purified to homogeneity by the pH mediated cell adsorption-desorption method and semi-preparative reversed-phase HPLC. The purification yield was 40.4% and the specific activity was increased by 2450-fold. It gave a single band and a single peak on SDS-PAGE and HPLC analysis, respectively. When subjected to electrospray LC-MS analysis, the protein was found to be highly pure and the molecular weight was determined as 4,618 Da. High concentration of the bacteriocin (> 50 micrograms/ml) showed good resistance to extremes of pH (1-12) and temperature (121 degrees C). Pediocin AcM was deduced to be a monomer with an intra-peptide disulfide bond from the results of reversed-phase analytical HPLC analyses after reduction, oxidation and trypsin digestion. P. acidilactici M inhibited a large number of bacteria, including Staphylococcus aureus, Listeria monocytogenes, Clostridium perfringens, Bacillus coagulans, B. cereus, and Aeromonas hydrophila.
authors.affiliation: Korea Food Research Institute, Kyongki-do, South Korea.
authors.forename: F B
authors.initials: FB
authors.lastname: Elegado
authors.forename: W J
authors.initials: WJ
authors.lastname: Kim
authors.forename: D Y
authors.initials: DY
authors.lastname: Kwon
mesh_terms: D001430:Bacteriocins / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010376:Pediococcus / Q000737:chemistry*; D013696:Temperature
chemical_list: D001430:Bacteriocins; D004220:Disulfides