Expression of interstitial collagenase (matrix metalloproteinase-1) is related to the activity of human endometriotic lesions.
OBJECTIVE
To determine whether interstitial collagenase (matrix metalloproteinase-1), known to play a pivotal role in the initiation of menstruation, contributes to the pathogenesis of endometriosis.


METHODS
Serial sections of peritoneal red and black endometriotic lesions, ovarian endometriotic cysts, and rectovaginal adenomyotic nodules were analyzed by in situ hybridization for the expression of matrix metalloproteinase-1 by silver staining for the integrity of the fibrillar extracellular matrix and by immunolabeling for the abundance of sex steroid receptors.


METHODS
Academic hospital and research laboratory.


METHODS
Premenopausal women undergoing laparoscopy for endometriosis.


METHODS
Biopsy of endometriotic lesions, combined with endometrium whenever possible.


METHODS
Expression of matrix metalloproteinase-1 messenger RNA (mRNA).


RESULTS
Matrix metalloproteinase-1 mRNA was expressed focally in red peritoneal and ovarian endometriosis irrespective of the phase of the menstrual cycle but was not detectable in black peritoneal and rectovaginal lesions. Foci of matrix metalloproteinase-1 expression closely correlated with matrix breakdown and with the absence of P receptors in adjacent epithelial cells.


CONCLUSIONS
Correlation of matrix metalloproteinase-1 expression with activity of endometriotic tissue suggests its involvement in tissue remodeling and bleeding, and possibly in the secondary shedding and reimplantation of endometriotic lesions.
Kokorine
I
I
International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Nisolle
M
M
Donnez
J
J
Eeckhout
Y
Y
Courtoy
P J
PJ
Marbaix
E
E
D000328:Adult; D017364:Collagenases / Q000235:genetics*; D004715:Endometriosis / Q000201:enzymology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D020781:Matrix Metalloproteinase 1; D008875:Middle Aged; D010053:Ovary / Q000201:enzymology; D010537:Peritoneum / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D016622:Silver Staining
D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D017364:Collagenases; D020781:Matrix Metalloproteinase 1