Cerebral glucose transporter expression in HIV infection.
Abnormalities in cerebral glucose metabolism have been demonstrated in patients with AIDS dementia complex (ADC), with increased consumption in early disease and decreased utilization in late stages. The basis of these changes is unknown. Accordingly, a pilot study was undertaken to determine whether levels of cerebral glucose transporters GLUT1 and GLUT3 were altered by HIV disease. Frontal gray and white matter membrane preparations from patients with HIV encephalitis (HIVE), HIV infection without parenchymal neuropathology, and non-infected controls were utilized in quantitative immunoblots to measure GLUT1 and GLUT3. Results were expressed as a ratio of glucose transporter to structural protein actin. Within-group patient variability was great, precluding statistically significant differences between groups for any one brain region. However, when data for gray and white matter was pooled, results obtained statistical significance, with levels of GLUT1 increasing in HIV infection without neuropathology, and declining with HIVE. Increased GLUT1 in HIV infection without parenchymal neuropathology may be important in the biology of increased glucose consumption described with early ADC. With progression to HIVE, reduced levels of glucose transporters may contribute to late stage hypometabolism.
Kovitz
C A
CA
Department of Pathology, Mount Sinai Medical Center, New York, NY 10029, USA.
Morgello
S
S
D015526:AIDS Dementia Complex / Q000150:complications / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000704:Analysis of Variance; D001921:Brain / Q000378:metabolism* / Q000821:virology*; D001927:Brain Diseases / Q000150:complications / Q000378:metabolism; D018792:Encephalitis, Viral / Q000150:complications / Q000378:metabolism; D005260:Female; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D015658:HIV Infections / Q000150:complications / Q000378:metabolism*; D006679:HIV Seropositivity / Q000150:complications / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins; D010865:Pilot Projects; D011153:Population; D011180:Postmortem Changes; D013997:Time Factors
D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; C495677:SLC2A1 protein, human; C495687:SLC2A3 protein, human