title: Comparison of two enzyme-linked immunosorbent assay tests for diagnosis of Helicobacter pylori infection in China.
abstract: An ELISA based on a pool of United States strains of Helicobacter pylori was compared with a newly developed ELISA based on a pool of Chinese strains. Both assays were tested using sera from 132 Chinese study subjects with biopsy-proven H. pylori infection. Using cutpoints designed to yield equal specificities of 94.9% in an uninfected control population, the sensitivity of the Chinese assay was 100.0%, compared to 97.7% for the United States assay (P = 0.25 by McNemar test). These results suggest that a H. pylori assay based on pooled antigens from United States strains will perform as well in the rural Chinese population as one based on antigens from Chinese strains.
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, Bethesda, Maryland 20892-7368, USA.
authors.forename: F D
authors.initials: FD
authors.lastname: Groves
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: J Y
authors.initials: JY
authors.lastname: Li
authors.forename: W C
authors.initials: WC
authors.lastname: You
authors.forename: Y S
authors.initials: YS
authors.lastname: Chang
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.forename: W D
authors.initials: WD
authors.lastname: Liu
authors.forename: C S
authors.initials: CS
authors.lastname: Rabkin
authors.forename: G I
authors.initials: GI
authors.lastname: Perez-Perez
authors.forename: M J
authors.initials: MJ
authors.lastname: Blaser
authors.forename: M H
authors.initials: MH
authors.lastname: Gail
mesh_terms: D000328:Adult; D002681:China / Q000453:epidemiology; D003431:Cross-Cultural Comparison; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005756:Gastritis / Q000175:diagnosis / Q000453:epidemiology*; D016481:Helicobacter Infections / Q000175:diagnosis / Q000453:epidemiology*; D016480:Helicobacter pylori; D006801:Humans; D015994:Incidence; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D012424:Rural Population / Q000706:statistics & numerical data*; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000453:epidemiology*; D014481:United States / Q000453:epidemiology