Bioactivation of promutagens by the unicellular green alga Chlamydomonas reinhardtii.
The aromatic amine 2-aminofluorene (2-AF) was activated by the intact Chlamydomonas reinhardtii cells to a mutagen that exhibited toxic and mutagenic effects comparable to those of the direct-acting mutagen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). There were different responses of the wildtype and repair-deficient strains to the toxic and mutagenic effect of 2-AF. The recombination repair plays a major role in repair of damages induced in the C. reinhardtii DNA by the aromatic amine promutagen 2-AF and the direct-acting mutagen MNNG. The 2-AF activation has also been analyzed by algal cells/microbe coincubation assay. This new assay is used in addition to animal microsome-metabolizing system (S9 fraction) and plant cell/microbe coincubation assay. This additional system is suitable for detection of environmental promutagens and their conversion to mutagens, mainly in aquatic environments.
Podstavková
S
S
Department of Genetics, Faculty of Science, Comenius University, Bratislava, Slovak Republic.
Vlcek
D
D
Miadoková
E
E
D000818:Animals; D001711:Biotransformation / Q000235:genetics; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000378:metabolism; D018920:Coculture Techniques; D005449:Fluorenes / Q000493:pharmacokinetics*; D008769:Methylnitronitrosoguanidine / Q000493:pharmacokinetics; D009152:Mutagenicity Tests; D009153:Mutagens / Q000493:pharmacokinetics*; D011355:Prodrugs / Q000493:pharmacokinetics*; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism
D005449:Fluorenes; D009153:Mutagens; D011355:Prodrugs; D008769:Methylnitronitrosoguanidine; C012177:2-aminofluorene