PCR primers to amplify 16S rRNA genes from cyanobacteria.
We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaea. Gene segments selectively retrieved from cyanobacteria and diatoms in unialgal but nonaxenic cultures and from cyanobionts in lichens could be directly sequenced. In the context of growing sequence databases, this procedure allows rapid and phylogenetically meaningful identification without pure cultures or molecular cloning. We demonstrate the use of this specific PCR in combination with denaturing gradient gel electrophoresis to probe the diversity of oxygenic phototrophic microorganisms in cultures, lichens, and complex microbial communities.
Nübel
U
U
Max Planck Institute for Marine Microbiology, Bremen, Germany.
Garcia-Pichel
F
F
Muyzer
G
G
D000458:Cyanobacteria / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D017377:Diatoms / Q000235:genetics; D008011:Lichens / Q000235:genetics; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D018087:Plastids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D013559:Symbiosis
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D010100:Oxygen