Complete coding regions of two novel HLA-B alleles detected by phototyping (PCR-SSP) in the British caucasoid population: B*5108 and B*5002.
Two previously reported PCR-SSP variants of the HLA-B locus, B51GAC and B45v, were investigated by RT-PCR cloning and nucleotide sequence analysis of their complete coding regions. They have been shown to correspond to the new alleles B*5108 and B*5002, both of which differ from the common B*5101 and B*5001 subtypes, respectively, by amino acid replacements at their alpha-2 domain alpha-helices. The primary structure of B*5002, intermediate between those of B*4501 and B*5001, raises further concern about the current classification of B*45 as a B12 rather than as a B*50 subtype.
Vilches
C
C
Servicio de Inmunología, Clínica Puerta de Hierro, Madrid, Spain.
Bunce
M
M
de Pablo
R
R
Murray
A K
AK
McIntyre
C A
CA
Kreisler
M
M
D000483:Alleles; D001483:Base Sequence; D004247:DNA; D015235:HLA-B Antigens / Q000235:genetics*; D059949:HLA-B51 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D006113:United Kingdom; D044465:White People / Q000235:genetics*
D015235:HLA-B Antigens; D059949:HLA-B51 Antigen; D004247:DNA