Comparison between micronucleated lymphocyte rates observed in healthy subjects and cancer patients.
Micronucleated cell rates were assessed in cytokinesis-blocked lymphocytes of 198 male and female healthy subjects (HS) not occupationally exposed to genotoxic risks and of 70 male and female cancer patients (CP) prior to any anticancer treatment. In the HS group, spontaneous micronucleated cell rates (MN cell rates) were 9.7 +/- 2.8 per 1000 binucleated lymphocytes and 9.8 +/- 3.1 for males and females respectively. In the CP group, spontaneous MN cell rates were 21.1 +/- 15.3 per 1000 binucleated lymphocytes and 19.1 +/- 11.2 for males and females respectively. Moreover, they were shown to have a large inter-individual variability in the two groups. The study of inter-individual variation factors showed that only tobacco could affect MN cell rate in HS whereas age and sex apparently had no significant effect. In the CP group, only age significantly affected MN cell rate, whereas sex, tobacco, alcohol, imaging techniques and tumour stage had no significant effect. There was no significant difference in the distribution of gender between HS and CP, whereas there was a significant difference in the distribution of age and tobacco between the two groups. The comparison of MN cell rates in 54 HS and 54 CP matched for age and sex showed a statistically significant difference. Spontaneous MN cell rates of these two populations reflect environmental exposure. Moreover, for CP it most probably refers to various cellular lesions and genetic damage.
Duffaud
F
F
Service d'Oncologie Médicale, CHU Timone Adultes, Marseille, France.
Orsière
T
T
Villani
P
P
Pelissier
A L
AL
Volot
F
F
Favre
R
R
Botta
A
A
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D008875:Middle Aged; D009367:Neoplasm Staging; D009369:Neoplasms / Q000235:genetics*; D011237:Predictive Value of Tests; D012016:Reference Values; D012737:Sex Factors; D012907:Smoking