title: Comparative analysis of bone marrow and venous blood isolates from gastrointestinal cancer patients for the detection of disseminated tumor cells using reverse transcription PCR.
abstract: We investigated 141 bone marrow and 104 venous blood isolates from gastrointestinal cancer patients with a cytokeratin (CK) 20-specific nested reverse transcription PCR for the detection of disseminated tumor cells at time of primary tumor resection. In colorectal cancer patients, 20 of 65 (31%) bone marrow and 9 of 52 (17%) venous blood isolates yielded a CK 20 mRNA-positive result in a stage-dependent manner. The detection rates for gastric cancer patients were 11 of 49 (22%) and 5 of 30 (17%) for bone marrow and venous blood, respectively. In pancreatic cancer patients, positive signals were found in advanced tumor stage. A duplex PCR system improved the feasibility of the test. After analyzing 70 sets of bone marrow and venous blood isolates from colorectal, gastric, and pancreatic cancer patients, we observed a higher detection rate in bone marrow isolates. Survival of patients with CK 20 mRNA-positive findings was significantly shorter than that of negatively tested patients.
authors.affiliation: Department of General Surgery and Thoracic Surgery, University Hospital Kiel, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Soeth
authors.forename: I
authors.initials: I
authors.lastname: Vogel
authors.forename: C
authors.initials: C
authors.lastname: Röder
authors.forename: H
authors.initials: H
authors.lastname: Juhl
authors.forename: J
authors.initials: J
authors.lastname: Marxsen
authors.forename: U
authors.initials: U
authors.lastname: Krüger
authors.forename: D
authors.initials: D
authors.lastname: Henne-Bruns
authors.forename: B
authors.initials: B
authors.lastname: Kremer
authors.forename: H
authors.initials: H
authors.lastname: Kalthoff
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001853:Bone Marrow / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000235:genetics; D053554:Keratin-20; D009360:Neoplastic Cells, Circulating; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D007381:Intermediate Filament Proteins; C507499:KRT20 protein, human; D053554:Keratin-20; D012333:RNA, Messenger