Langmuir films from antibodies based on amphiphilic polyelectrolytes.
A technique for forming Langmuir films from antibodies based on an amphiphilic polyelectrolyte was developed. The physicochemical and immunochemical properties of the Langmuir films obtained were studied. The interaction of HBsAg with the films was found to be described by a model with one binding site, whereas that of HBsAg with antibodies adsorbed on a polystyrene plate, by a model with a positive cooperativity. The use of the novel Langmuir films from antibodies increases the sensitivity of the immunoenzyme assay.
Babitskaya
J I
JI
Research Center of Molecular Diagnostics and Therapy, Moscow.
Budashov
I A
IA
Chernov
S F
SF
Kurochkin
I N
IN
Doroshenko
N V
NV
Zubov
S V
SV
D000818:Animals; D004573:Electrolytes / Q000737:chemistry* / Q000378:metabolism; D006041:Goats; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000378:metabolism; D008567:Membranes, Artificial; D020366:Methylmethacrylate; D008768:Methylmethacrylates / Q000737:chemistry; D009994:Osmolar Concentration; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
D004573:Electrolytes; D006514:Hepatitis B Surface Antigens; D007140:Immunoglobulin Fab Fragments; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D011108:Polymers; D013501:Surface-Active Agents; D020366:Methylmethacrylate