Selective usage of defined TCRBV genes in response to filarial antigens.
The characterization of T cell reactivities that are prone to down-modulation by filarial parasites is central to understanding how these nematodes can survive for long periods of time within their human host and to design appropriate immunoprophylactic measures. In the present study, TCRBV gene usage was analyzed in response to filarial antigens by PCR using a panel of TCRBV gene segment family-specific oligonucleotide primers. Analysis of individuals highly responsive to Brugia malayi adult worm antigen (BmA) (n = 4) indicated that following stimulation with BmA a maximum of four TCRBV gene families were over-represented in each subject. Those were TCRBV2, 9, 19 and 23 in subject 1; TCRBV8, 9 and 16 in subject 2; TCRBV2, 8, 9 and 11 in subject 3; and TCRBV13 and 23 in subject 4. The analysis of one subject who was unresponsive to BmA before but regained responsiveness after diethylcarbamazine treatment revealed that there was no overexpression of a particular TCRBV gene family before chemotherapy, whereas after chemotherapy three TCRBV gene families (TCRBV8, 16 and 19) were found to be overexpressed. Complementarity determining region 3 size analysis of a selection of the overexpressed TCRBV genes displayed oligoclonality in some of the observed expansions. Together these observations show that limited T cell subpopulations are clonally amplified in BmA-stimulated peripheral blood mononuclear cells of filarial responder subjects, possibly driven by a restricted number of antigens.
Sartono
E
E
Department of Parasitology, Leiden University, The Netherlands.
van Eggermond
M C
MC
Kurniawan
A
A
Maizels
R M
RM
van den Elsen
P J
PJ
Yazdanbakhsh
M
M
D000293:Adolescent; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D017178:Brugia malayi / Q000254:growth & development / Q000276:immunology*; D002999:Clone Cells / Q000378:metabolism; D004049:Diethylcarbamazine / Q000494:pharmacology; D004605:Elephantiasis, Filarial / Q000235:genetics / Q000276:immunology*; D005260:Female; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000187:drug effects; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics; D008297:Male; D008875:Middle Aged; D005810:Multigene Family / Q000187:drug effects / Q000276:immunology; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000187:drug effects / Q000235:genetics* / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000378:metabolism*
D000947:Antigens, Helminth; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004049:Diethylcarbamazine