title: Synthesis of glycosyl-trehaloses by cyclomaltodextrin glucanotransferase through the transglycosylation reaction.
abstract: Cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus produced a series of glycosyl-trehaloses through the transglycosylation reaction with cyclomaltohexaose as the glycosyl donor and trehalose as its acceptor. After beta-amylase treatment, five species of glycosyl-trehaloses were isolated by column chromatography. After chemical and enzymatic analyses, it was concluded that these oligosaccharides were alpha-maltosyl alpha-D-glucopyranoside, alpha-maltotriosyl alpha-D-glucopyranoside, alpha-maltosyl alpha-maltoside, alpha-maltotriosyl alpha-maltoside, and alpha-maltotriosyl alpha-maltotrioside. These were not hydrolyzed by salivary amylase, artificial gastric juice, or pancreatic amylase, however they were hydrolyzed by enzymes of the small intestine.
authors.affiliation: Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kurimoto
authors.forename: A
authors.initials: A
authors.lastname: Tabuchi
authors.forename: T
authors.initials: T
authors.lastname: Mandai
authors.forename: T
authors.initials: T
authors.lastname: Shibuya
authors.forename: H
authors.initials: H
authors.lastname: Chaen
authors.forename: S
authors.initials: S
authors.lastname: Fukuda
authors.forename: T
authors.initials: T
authors.lastname: Sugimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Tsujisaka
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000737:chemistry / Q000378:metabolism*; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D055095:Optics and Photonics; D014199:Trehalose / Q000138:chemical synthesis* / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D014199:Trehalose; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose