Hepatitis B virus binding to leucocyte plasma membranes utilizes a different region of the preS1 domain to the hepatocyte receptor binding site and does not require receptors for opsonins.
A quantitative assay of hepatitis B virus (HBV) binding to hepatocyte plasma membranes was adapted to show that leucocyte plasma membranes bind serum-derived HBV saturably, and that this binding is inhibited using synthetic peptides representative of the large envelope protein of HBV. Using a panel of ligand-blocking monoclonal antibodies (mAb) to opsonin receptors, it was shown that the three classes of Fc gamma R and CR3 are not major receptors for HBV on leucocytes or hepatocytes. It was also shown that HBV does not utilize the receptor for IgA, Fc alpha R, for attachment to leucocytes, despite reported sequence homology between the large envelope protein of HBV and the Fc portion of human IgA. Evidence is presented that the receptor for HBV on leucocytes may differ from the hepatocyte receptor(s), based on synthetic peptide inhibition assays of HBV binding. Furthermore, it was observed that glycosaminoglycans influence the HBV-liver and leucocyte interactions, providing evidence that HBV attachment may be a multi-stage process.
Atkins
G J
GJ
Division of Haematology, University of Adelaide, Australia.
Qiao
M
M
Coombe
D R
DR
Gowans
E J
EJ
Ashman
L K
LK
D000328:Adult; D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002462:Cell Membrane / Q000821:virology; D006025:Glycosaminoglycans / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000821:virology*; D008099:Liver / Q000166:cytology / Q000821:virology*; D011498:Protein Precursors / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism*
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D006025:Glycosaminoglycans; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D011971:Receptors, Immunologic; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; C091196:opsonin receptor; C050806:presurface protein 1, hepatitis B surface antigen