title: Immunoreactivity of mycobacterial 70 kDa protein in different physico-chemical forms.
abstract: The immunoreactivity of 70 kDa culture filtrate protein of Mycobacterium tuberculosis H37 Ra has been examined as such and by increasing its hydrophobicity through its conjugation to stearic acid ester (70 kDa-FAester). The cell mediated immune responses produced by 70 kDa-FAester encapsulated in phosphatidylcholine (PC) liposomes were significantly higher particularly at the 3rd week post immunization (wk p. im.) than that produced by the 70 kDa protein in PC liposomes, whereas humoral immune responses were non-significant. Further, these immune responses were comparable to that elicited by 70 kDa protein complexed with Freund's incomplete adjuvant (70 kDa-FIA). Results of this study suggest that changes in physicochemical nature of 70 kDa protein influences both the humoral and cellular immunoreactivity.
authors.affiliation: Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh.
authors.forename: I
authors.initials: I
authors.lastname: Verma
authors.forename: D
authors.initials: D
authors.lastname: Yadav
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.forename: R
authors.initials: R
authors.lastname: Sinha
authors.forename: G K
authors.initials: GK
authors.lastname: Khuller
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D008081:Liposomes / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010713:Phosphatidylcholines
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; D001426:Bacterial Proteins; D008081:Liposomes; D010713:Phosphatidylcholines