Comparative sensitivity of human erythrocytes and lymphocytes to sonolysis by 1-MHz ultrasound.
Many studies of ultrasonic hemolysis have used erythrocytes; other blood cells are less well studied. The hypothesis tested was that human lymphocytes, being large and relatively fragile, are more sensitive to sonolysis than are erythrocytes at equivalent cell concentrations. Human lymphocytes (RPMI 1788) grown in vitro and erythrocytes obtained by venipuncture were used at a nominal cell concentration of 2.5 x 10(6) cells/mL. Cells were contained in rotated (200 rpm) exposure vessels and were exposed/sham-exposed to 1-MHz continuous-wave ultrasound for 60 s. Cell lysis was determined by hemacytometer counts of aliquots taken before/after treatment. The hypothesis was supported; the mean levels of lysis in insonated lymphocyte and erythrocyte preparations were 89.8% +/- 0.6% and 78.9% +/- 3.1%, respectively. This difference was significant at p < 0.005.
Miller
M W
MW
Department of Obstetrics and Gynecology, School of Medicine and Dentistry, University of Rochester, NY 14642, USA.
Brayman
A A
AA
D002478:Cells, Cultured; D004912:Erythrocytes; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes; D014465:Ultrasonics / Q000009:adverse effects*