title: The evidence of clonal evolution with monosomy 7 in aplastic anemia following granulocyte colony-stimulating factor using the polymerase chain reaction.
abstract: We present here the case of a Japanese female patient with aplastic anemia who developed monosomy 7 and clonal evolution following a treatment with recombinant human granulocyte colony-stimulating factor (rhG-CSF). At the onset of aplastic anemia, cytogenetic analysis was 46, XX and X-inactivation/methylation analysis revealed a polyclonal pattern. After 4 months of administration of rhG-CSF, she had 45, XX, -7 and a clonal pattern, although there were no morphological evidence of a myelodysplastic syndrome or leukemia. The ratio of monosomy 7 to normal analyzed by fluorescence in situ hybridization decreased after discontinuation of rhG-CSF and there were still no dysplastic changes and/or increased numbers of blasts. These results indicate that the acquisition of monosomy 7 following rhG-CSF treatment dose not always cause clonal evolution to induce hematological malignancies.
authors.affiliation: The First Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan. t.vulliamy@rpms.ac.uk
authors.forename: E
authors.initials: E
authors.lastname: Yamazaki
authors.forename: H
authors.initials: H
authors.lastname: Kanamori
authors.forename: J
authors.initials: J
authors.lastname: Taguchi
authors.forename: H
authors.initials: H
authors.lastname: Harano
authors.forename: H
authors.initials: H
authors.lastname: Mohri
authors.forename: T
authors.initials: T
authors.lastname: Okubo
mesh_terms: D000741:Anemia, Aplastic / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D005075:Biological Evolution; D001854:Bone Marrow Cells / Q000473:pathology; D002897:Chromosomes, Human, Pair 7; D005260:Female; D005819:Genetic Markers; D016179:Granulocyte Colony-Stimulating Factor / Q000009:adverse effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007564:Japan; D007621:Karyotyping; D009006:Monosomy; D009190:Myelodysplastic Syndromes / Q000235:genetics; D010735:Phosphoglycerate Kinase / Q000235:genetics; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000009:adverse effects
chemical_list: D005819:Genetic Markers; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D010735:Phosphoglycerate Kinase