Complete sequence and transposon mutagenesis of the BamHI J fragment of Cydia pomonella granulosis virus.
The BamHI J fragment of Cydia pomonella granulosis virus was subcloned and subjected to transposon mutagenesis in Escherichia coli using a Tn3 derivative. After screening by restriction endonuclease digestion and polymerase chain reaction (PCR), 44 clones were selected representing insertions every 100 to 300 bp. The complete sequence was compiled and the transposon insertion sites mapped precisely by sequencing. Analysis of the sequence revealed the presence of 7 potential open reading frames (ORFs). The BamHI J fragment was already known to encode IAP and OPDV-E6. Three other ORFs encode products similar to known proteins, viz. an Autographa californica nuclear polyhedrosis virus 8.6 kDa protein, a Lymantria dispar nuclear polyhedrosis virus 34 kDa protein, and vertebrate reovirus omega-1 proteins. The ORF with similarity to omega-1 is also similar to baculovirus p10 proteins. In both cases, the similarity occurs in regions likely to form a coiled-coil structure.
Kang
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Department of Biology, Imperial College of Science, Technology and Medicine, London, UK.
Crook
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Winstanley
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O'Reilly
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D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D004926:Escherichia coli / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
D017931:DNA Primers; D004279:DNA, Viral; D014764:Viral Proteins; D015245:Deoxyribonuclease BamHI