Planar chromatographic and liquid chromatographic analysis of thiamphenicol in bovine and human plasma.
A planar chromatographic (PC) method was developed to determine thiamphenicol in human and bovine plasma with florphenicol as internal standard. The performance of the method was compared with that of a liquid chromatographic method. Recovery of extraction method developed for plasma was 81.51 +/- 2.85%. Reproducibilities of the PC assay performed with various PC plates were also good.
Dreassi
E
E
Università di Siena, Dipartimento Farmaco Chimico Tecnologico, Italy.
Corbini
G
G
Ginanneschi
V
V
Corti
P
P
Furlanetto
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003720:Densitometry; D004350:Drug Residues / Q000378:metabolism*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; C035534:florfenicol; D013839:Thiamphenicol