HMG1 protein inhibits the translesion synthesis of the major DNA cisplatin adduct by cell extracts.
When situated in a fork-like synthetic DNA replication substrate, the 1,2-intrastrand crosslink at the d(GpG) site, the most frequent adduct formed in the reaction between DNA and the anticancer drug cisplatin (cis-diamminedichloroplatinum (II)), is efficiently bypassed by eukaryotic cell extracts. We show here that the rat high-mobility-group protein 1 (HMG1) binds preferentially to the platinated fork-like synthetic DNA and inhibits the translesion synthesis. The same protein, but without the acidic tail, inhibits also the translesion synthesis. These results suggest that HMG proteins might contribute to the sensitivity of cells to cisplatin by directly affecting DNA replication.
Hoffmann
J S
JS
Institut de Pharmacologie et de Biologie Structurale, CNRS, 205 route de Narbonne, Toulouse cedex, 31077, France.
Locker
D
D
Villani
G
G
Leng
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D016466:CHO Cells; D002457:Cell Extracts; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D006224:Cricetinae; D004247:DNA / Q000737:chemistry / Q000187:drug effects; D018736:DNA Adducts / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006609:High Mobility Group Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D051381:Rats
D000970:Antineoplastic Agents; D002457:Cell Extracts; D018736:DNA Adducts; D006609:High Mobility Group Proteins; C057270:cisplatin-DNA adduct; D004247:DNA; D002945:Cisplatin