The effect of interferon-gamma on genetic immunization.
The effect of co-inoculation of a plasmid vector expressing the rabies virus glycoprotein and an additional vector encoding mouse interferon (IFN)-gamma on the development of an antigen specific B and T helper cell response was tested upon intramuscular inoculation of mice. The effect of IFN-gamma was dependent on the promoter driving expression of the viral antigen. The immune responses to antigen-expressing vector carrying a viral promoter such as the SV40 early promoter or the major histocompatibility (MHC) class I promoter were reduced in presence of IFN-gamma while the B and T helper cell response to a vector expressing the antigen under the control of the MHC class II promoter was not affected by this cytokine.
Xiang
Z Q
ZQ
Wistar Institute, Philadelphia, PA 19104, USA.
He
Z
Z
Wang
Y
Y
Ertl
H C
HC
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D000998:Antiviral Agents / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D004279:DNA, Viral / Q000276:immunology; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D011401:Promoter Regions, Genetic; D011820:Rabies virus / Q000235:genetics / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000998:Antiviral Agents; D004279:DNA, Viral; D006023:Glycoproteins; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus; D007371:Interferon-gamma