title: Neuroprotective effect of graded postischemic reoxygenation in spinal cord ischemia in the rabbit.
abstract: Early ischemia/reperfusion-induced changes of four phospholipid compounds bound to the inner cell membrane leaflet, i.e., phosphatidic acid, inositol phospholipids, serine phospholipids, and ethanolamine plasmalogens, were studied in a model of spinal cord ischemia in the rabbit during normoxic and graded postischemic reoxygenation. Light and electron microscopic analysis after normoxic reoxygenation disclosed neuronal membrane argyrophilia of the interneuronal pool located in lamina VII of L4-L6 segments. The number of small neurons (10-25 microm in diameter) affected by somatodendritic argyrophilia was greatly reduced, and concomitantly the ultrastructure of the endoplasmic reticulum, mitochondria, and Golgi complexes remained almost undamaged when graded postischemic reoxygenation had been applied. A statistically significant increase of phosphatidylserine and ethanolamine plasmalogen levels, and a decrease of phosphatidic acid, were detected after a short-lasting graded postischemic reoxygenation. The formation of thiobarbituric acid-reactive substances was significantly reduced during 60 min of graded postischemic reoxygenation and remained close to control or ischemic levels. The present data indicate that graded postischemic reoxygenation, which is considered to be neuroprotective, can prevent neuronal argyrophilia and the development of reperfusion-induced alterations of organelles. Moreover, reoxygenation can positively modify ischemia-induced changes of some membrane-bound phospholipids.
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovak Republic.
authors.forename: N
authors.initials: N
authors.lastname: Lukácová
authors.forename: M
authors.initials: M
authors.lastname: Marsala
authors.forename: G
authors.initials: G
authors.lastname: Halát
authors.forename: J
authors.initials: J
authors.lastname: Marsala
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000648:ultrastructure; D007511:Ischemia / Q000473:pathology*; D019194:Ischemic Preconditioning; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008854:Microscopy, Electron; D010743:Phospholipids / Q000378:metabolism; D011817:Rabbits; D015424:Reperfusion; D013116:Spinal Cord / Q000098:blood supply* / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances
chemical_list: D008563:Membrane Lipids; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances