Assay for valproic acid and its E-delta2 metabolite in rat plasma by capillary gas chromatography without prior derivatization.
A new and improved gas chromatographic assay method for valproic acid and a metabolite, E-delta2 valproic acid, in rat plasma has been developed. The assay has sufficient sensitivity to measure free levels of the parent drug and metabolite. By employing a Stabilwax-DA capillary column, symmetrical chromatographic peaks were obtained without the need for prior derivatization. Standard curves for valproic acid were linear from 0.1 to 640 microg/ml. Standard curves for the metabolite were linear from 0.1 to 556 microg/ml.
Carlin
A
A
Biopharmaceutics Research Branch, Center for Drug Evaluation and Research, Food and Drug Administration, Rockville, MD 20850, USA.
Simmons
J
J
D000818:Animals; D000927:Anticonvulsants / Q000097:blood*; D002849:Chromatography, Gas; D004355:Drug Stability; D005229:Fatty Acids, Monounsaturated / Q000097:blood*; D051381:Rats; D014635:Valproic Acid / Q000097:blood*
D000927:Anticonvulsants; D005229:Fatty Acids, Monounsaturated; C014168:2-propyl-2-pentenoic acid; D014635:Valproic Acid