Role of (ADP-ribose)n catabolism in DNA repair.
Poly(ADP-ribose) is a reversible covalent-modifier of chromosomal proteins in eukaryotic cells. The function of poly(ADP-ribose) is not clear, although it has been suggested to be involved in the regulation of DNA transactions such as replication, repair, and transcription. Here we describe a specific competitive inhibitor of poly(ADP-ribose) glycohydrolase, a macrocircular ellagitannin oenothein B, and a nuclear system prepared from synchronized HeLa S3 cells at mid-G1 phase that enable us to examine the role of poly(ADP-ribose) catabolism in DNA repair. The results suggest that poly(ADP-ribose) is capable of generating ATP by the concerted action of poly(ADP-ribose) glycohydrolase and ADP-ribose pyrophosphorylase and that this ATP enables repair DNA synthesis.
Maruta
H
H
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Shinjuku-ku, Japan.
Matsumura
N
N
Tanuma
S
S
D000255:Adenosine Triphosphate / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002474:Cell-Free System; D004260:DNA Repair; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047348:Hydrolyzable Tannins; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D013634:Tannins / Q000494:pharmacology
D004791:Enzyme Inhibitors; D047348:Hydrolyzable Tannins; D013634:Tannins; C080077:oenothein B; D011064:Poly Adenosine Diphosphate Ribose; D000255:Adenosine Triphosphate; D006026:Glycoside Hydrolases; C022407:poly ADP-ribose glycohydrolase