Histopathological changes in the testes of prepubertal male rats after chronic administration of cocaine.
OBJECTIVE
This study was conducted to evaluate histopathologic findings in the testes of prepubertal male rats after long-term cocaine exposure.


METHODS
At 25 days of age, male Sprague-Dawley rats were administered cocaine hydrochloride daily (15 mg/kg body weight corresponding to an average single dose for a heavy cocaine user). The treatment was continued for 100 days when all the rats were sacrificed. Morphological analysis of the testes were assessed by qualitative and quantitative histological means.


RESULTS
In all the groups, a minimum of 5 to 10 representative seminiferous tubules were examined. The mean diameter of the seminiferous tubules was less in the treated group than in their respective controls (p < 0.05). The thickness of the germinal epithelium was much reduced in the cocaine-treated groups when compared with their controls (p < 0.05). The number of degenerating germ cells was greater in the treated group than in the controls. There was evidence of failure to release the mature spermatids in the treated groups. There was no evidence of sloughed Sertoli cells or germ cells in the tubular lumen or the epididymis.


CONCLUSIONS
There were distinct histopathological changes noted after chronic administration of cocaine. These changes are characteristic of toxic effects on the testes, but the exact mechanism is not clear. Further studies are underway in our laboratory to delineate the exact mechanism of action by cocaine on the testes.
Li
H
H
Department of Urology, Wayne State University, Detroit, Michigan 48201, USA.
George
V K
VK
Bianco
F J
FJ
Lawrence
W D
WD
Dhabuwala
C B
CB
D000818:Animals; D003042:Cocaine / Q000008:administration & dosage* / Q000633:toxicity*; D004334:Drug Administration Schedule; D007279:Injections, Subcutaneous; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012670:Seminiferous Epithelium / Q000187:drug effects / Q000473:pathology; D012741:Sexual Maturation / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000473:pathology*
D003042:Cocaine