Detection and characterization of Leishmania in tissues of patients with post kala-azar dermal leishmaniasis using a specific monoclonal antibody.
Sections from skin lesions and draining lymph nodes of patients with post kala-azar dermal leishmaniasis were examined using an immunoperoxidase method and a monoclonal antibody directed against Leishmania donovani. Parasites were detected in 22 of 25 biopsies (88%). In parallel sections stained by haematoxylin and eosin, parasites were detected in only 5 of 25 of the biopsies (20%). The demonstration of whole parasites or parasite antigen is diagnostic.
Ismail
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A
Centre of Medical Parasitology, University of Copenhagen, Denmark.
Kharazmi
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Permin
H
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el Hassan
A M
AM
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007893:Leishmania donovani / Q000276:immunology / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D007898:Leishmaniasis, Visceral / Q000150:complications*; D008198:Lymph Nodes / Q000469:parasitology; D012867:Skin / Q000469:parasitology
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan