Formation of homo- and heterooligomeric supramolecular structures by D-glyceraldehyde-3-phosphate dehydrogenase and lactate dehydrogenase in reversed micelles of aerosol OT in octane.
The supramolecular structure of oligomeric enzymes can be specifically regulated by changing the size of an inner cavity of Aerosol OT reversed micelles in octane. Both D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and lactate dehydrogenase (LDH) reveal an ability to exist and function in monomeric, dimeric and tetrameric forms (homooligomers). Various heterooligomeric complexes, in particular, GAPDH monomer--LDH monomer, GAPDH dimer--LDH tetramer were detected in reversed micelles.
Levashov
A V
AV
Department of Chemistry, Moscow State University, Russia.
Ugolnikova
A V
AV
Ivanov
M V
MV
Klyachko
N L
NL
D000818:Animals; D002384:Catalysis; D004143:Dioctyl Sulfosuccinic Acid; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances; D008823:Micelles; D009795:Octanes; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers; D011487:Protein Conformation; D011817:Rabbits; D012997:Solvents; D013501:Surface-Active Agents
D046911:Macromolecular Substances; D008823:Micelles; D009795:Octanes; D010446:Peptide Fragments; D011108:Polymers; D012997:Solvents; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; C068142:glyceraldehyde 3-phosphate dehydrogenase (304-313); D007770:L-Lactate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C026728:octane