title: The proliferating cell nuclear antigen (PCNA) gene family in Zea mays is composed of two members that have similar expression programmes.
abstract: PCNA is an auxilliary protein for DNA polymerase delta whose function is to increase both polymerase activity and processivity. We have previously reported the isolation of a maize cDNA clone encoding a homologue of PCNA. Here we report the identification of a second maize PCNA cDNA clone. The nucleic acid sequence of both clones is almost identical in the coding sequences, showing 94% identity, but differs by approximately 40% in the 5' and 3' non-translated regions. Maize genomic Southern blots probed with the complete cDNAs and gene-specific probes revealed that maize contains two PCNA genes. Northern blots of RNA extracted from different plant tissues show that both genes are equally expressed in proliferating tissues.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F. ilopez@servidor.unam.mx
authors.forename: I
authors.initials: I
authors.lastname: López
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.forename: J
authors.initials: J
authors.lastname: Vázquez
authors.forename: P J
authors.initials: PJ
authors.lastname: Hussey
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018809:Proliferating Cell Nuclear Antigen