Alanyl-tRNA synthetase gene of the extreme acidophilic chemolithoautotrophic Thiobacillus ferrooxidans is highly homologous to alaS genes from all living kingdoms but cannot be transcribed from its promoter in Escherichia coli.
The alaS gene of Thiobacillus ferrooxidans has been cloned and sequenced and its expression in Escherichia coli and T. ferrooxidans analysed. The same genomic organization to that in E. coli (recA-recX-alaS) has been found in T. ferrooxidans. The recA and alaS genes cannot be transcribed from their own promoters in E. coli. In addition to the well-known homology at the protein level between AlaS proteins from various organisms, a strong homology was found between all the known alaS genes from bacteria, archaea and eucarya. Two regions, one of which corresponds to the catalytic core, are particularly well-conserved at the nucleotide sequence level, a possible indication of strong constraints during evolution on these parts of the genes.
Guiliani
Nicolas
N
Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et de Microbiologie, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
Bengrine
Abderrahmane
A
Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et de Microbiologie, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
Borne
Francoise
F
Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et de Microbiologie, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
Chippaux
Marc
M
Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et de Microbiologie, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
Bonnefoy
Violaine
V
Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et de Microbiologie, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
D000412:Alanine-tRNA Ligase / Q000235:genetics*; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013855:Thiobacillus / Q000235:genetics*; D014158:Transcription, Genetic
D011938:Rec A Recombinases; D000412:Alanine-tRNA Ligase