Cloning and sequence analysis of the fimbriae associated protein (fap) gene from Actinobacillus actinomycetemcomitans.
Fimbrial-associated protein, an attachment factor of Actinobacillus actinomycetemcomitans, was genetically analysed by cloning. The plasmid obtained was found to harbor a 1.7kb fragment which contained a 228bp open reading frame encoding 76 amino acids (7.970kDa). The fimbriae associated protein gene (fap) was strongly expressed in fimbriated A. actinomycetemcomitans 310a but not in non-fimbriated strains.
Ishihara
K
K
Department of Microbiology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba, 261, Japan.
Honma
K
K
Miura
T
T
Kato
T
T
Okuda
K
K
D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C108544:fimbriae-associated protein, Actinobacillus; D036781:Fimbriae Proteins