Monoclonal antibodies against pupa-specific surface antigens of Sarcophaga peregrina (flesh fly) hemocytes.
Monoclonal antibodies were raised against pupal hemocytes of Sarcophaga peregrina. Four of the antibodies tested bound specifically to the surface of live pupal hemocytes, and these hemocytes appeared to be granulocytes from their morphology. The antigen recognized by one of the antibodies, MAb 9C8, was considered to be a membrane protein with a molecular mass of 120-kDa. Another antibody, MAb 9F3, was found to recognize possibly the same 120-kDa protein and two cytoplasmic proteins commonly present in both pupal and larval hemocytes.
Hori
S
S
Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Japan.
Kobayashi
A
A
Natori
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D004175:Diptera / Q000166:cytology / Q000254:growth & development / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D006434:Hemocytes / Q000276:immunology*; D019476:Insect Proteins / Q000276:immunology*; D008970:Molecular Weight; D011679:Pupa
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D019476:Insect Proteins