title: Gluten-sensitive enteropathy in Irish setter dogs: characterisation of jejunal microvillar membrane proteins by two-dimensional electrophoresis.
abstract: This study investigated whether gluten-sensitive enteropathy (GSE) in Irish setter dogs was associated with underlying structural abnormalities of microvillar membrane proteins. Jejunal biopsies taken from eight-month-old GSE-affected dogs reared on a normal, gluten-containing diet exhibited partial villous atrophy and contained more intra-epithelial lymphocytes than controls. The morphological abnormalities were reversed by feeding a gluten-free diet for five months and the changes were accompanied by an increase in the mucosal activity of the microvillar hydrolases, particularly aminopeptidase N and dipeptidyl aminopeptidase IV, which reverted to pre-treatment levels after a gluten challenge. Two-dimensional electrophoresis of microvillar membrane proteins isolated from GSE-affected dogs revealed an essentially normal protein map that was comparable to controls. The exception was an intense 85 kDa protein spot that diminished when the affected dogs were fed a gluten-free diet and re-intensified after a gluten challenge.
authors.affiliation: Department of Gastroenterology, Manchester Royal Infirmary.
authors.forename: P W
authors.initials: PW
authors.lastname: Pemberton
authors.forename: R W
authors.initials: RW
authors.lastname: Lobley
authors.forename: R
authors.initials: R
authors.lastname: Holmes
authors.forename: S H
authors.initials: SH
authors.lastname: Sørensen
authors.forename: R M
authors.initials: RM
authors.lastname: Batt
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000626:Aminopeptidases / Q000032:analysis; D000818:Animals; D001284:Atrophy / Q000473:pathology / Q000662:veterinary; D001706:Biopsy / Q000662:veterinary; D002446:Celiac Disease / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods / Q000662:veterinary; D005983:Glutens / Q000494:pharmacology; D006867:Hydrolases / Q000032:analysis; D007583:Jejunum / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure*; D008214:Lymphocytes / Q000473:pathology; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D008871:Microvilli / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D008565:Membrane Proteins; D005983:Glutens; D006867:Hydrolases; D000469:Alkaline Phosphatase; D000626:Aminopeptidases