Ribotyping and DNA macrorestriction analysis of isolates of Burkholderia pseudomallei from cases of melioidosis in Malaysia.
Forty-nine isolates of Burkholderia pseudomallei from sporadic cases of melioidosis in Malaysia over the past 18 years were examined by BamHI ribotyping and pulsed-field gel electrophoresis (PFGE) of XbaI digests of total deoxyribonucleic acid (DNA). Twenty-four patients had septicaemic melioidosis with a mortality of 70%; mortality in the non-septicaemic disease was 16%. Five ribotype patterns were identified, 2 of which accounted for 90% of all isolates. PFGE revealed a number of different strains within these ribotypes, but some pairs of isolates from unrelated cases gave closely similar DNA profiles. These results are in agreement with Australian studies which showed a high prevalence of a few ribotypes of B. pseudomallei which are further divisible by genotyping, in areas where melioidosis is endemic.
Vadivelu
J
J
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Puthucheary
S D
SD
Mifsud
A
A
Drasar
B S
BS
Dance
D A
DA
Pitt
T I
TI
D000328:Adult; D000368:Aged; D016957:Burkholderia pseudomallei / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008296:Malaysia; D008297:Male; D008554:Melioidosis / Q000382:microbiology*; D008875:Middle Aged; D015183:Restriction Mapping
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015245:Deoxyribonuclease BamHI