Cell cycle specificity of Fas-mediated apoptosis in WIL-2 cells.
Antibodies to Fas/APO1 receptor induce effective apoptosis in WIL-2 cells of the human B-lymphoid line. Quantitative assessment of the extent of the death in cells synchronized by thymidine block revealed a significant increase in their sensitivity to the cytocidal effect mediated by Fas/APO1 during the G1 phase of the cell cycle. Western analysis of the content of the p53 antigen in the cytoplasm and nuclei of the cells showed that the Fas/APO1-induced death is accompanied by massive translocation of the p53 from the cytoplasm to the nucleus. These findings suggest that cell vulnerability to the Fas/APO1-mediated apoptosis is subjected to regulation by cell cycle-dependent mechanisms, one of which is probably the function of the p53 antigen.
Beletskaya
I V
IV
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Science, Puschino, Moscow region, Russian Federation.
Nikonova
L V
LV
Beletsky
I P
IP
D000906:Antibodies / Q000494:pharmacology; D017209:Apoptosis; D001402:B-Lymphocytes / Q000166:cytology*; D015153:Blotting, Western; D002453:Cell Cycle; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D016193:G1 Phase; D006801:Humans; D007700:Kinetics; D008898:Mimosine / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D019014:fas Receptor / Q000276:immunology / Q000502:physiology*
D000906:Antibodies; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor; D008898:Mimosine