Simple color tests based on an alanyl peptidase reaction which differentiate Listeria monocytogenes from other Listeria species.
The hydrolysis of DL-alanine-beta-naphthylamide and D-alanine-p-nitroanilide for identification of Listeria spp. has been studied with 227 cultures. All species of Listeria, except L. monocytogenes, hydrolyzed these substrates. The reactions were detected by simple chromogenic reactions and could substitute for the CAMP test.
Clark
A G
AG
Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada. gavin.clark@utoronto.ca
McLaughlin
J
J
D000409:Alanine / Q000031:analogs & derivatives / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D001431:Bacteriological Techniques; D003116:Color; D010450:Endopeptidases / Q000378:metabolism*; D008087:Listeria / Q000145:classification; D008089:Listeria monocytogenes / Q000145:classification / Q000378:metabolism*; D013045:Species Specificity; D013379:Substrate Specificity
D000814:Aniline Compounds; C004813:alanine-4-nitroanilide; C028104:alanine-beta-naphthylamide; D010450:Endopeptidases; C027864:tri-alanyl peptidase; D000409:Alanine