Characterization of the gene encoding mouse platelet glycoprotien Ib beta.
Platelet glycoprotein (GP) Ib/IX/V complex is a major receptor for von Willebrand factor (vWF), which mediates platelet adhesion and aggregation under high shear stress conditions. It is composed of GPIb alpha, GPIb beta, GPIX, and GPV. All subunits for the human receptor have been cloned and characterized. However, the function of GPIb beta is still elusive. To obtain further information of GPIb beta, we have determined the genomic sequence of mouse GPIb beta (1466 bp). The deduced amino acid sequence (206aa) was 88% identical to the human GPIb beta protein. All cysteine residues, putative N-linked glycosylation site (Asn41), and putative phosphorylation site (Ser166) were conserved. The promoter region contained putative GATA and ets binding motif implicated in megakaryocytic expression. Mouse GPIb beta also contained a leucine-rich glycoprotein (LRG) sequence of 24 amino acids same as human GPIb beta.
Kitaguchi
T
T
Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
Murata
M
M
Anbo
H
H
Moriki
T
T
Ikeda
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001792:Blood Platelets / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics*; D017422:Sequence Analysis, DNA
D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; C028254:von Willebrand factor receptor