title: Treatment of dystrophic epidermolysis bullosa with autologous meshed split-thickness skin grafts and allogeneic cultured keratinocytes.
abstract: A 5-year-old boy with dystrophic epidermolysis bullosa (DEB), type generalized mitis, presented with a 2-year-old non-healing skin defect on the presternal and abdominal region. After debridement up to the middle dermis, the defect was grafted with autologous meshed split-thickness grafts from non-involved skin from the thigh and covered with allogeneic keratinocytes. The described method is a valuable therapeutic option in DEB patients who have donor skin areas with a preserved structure of the basement membrane zone.
authors.affiliation: Department of Dermatology, University Hospital Gent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Verplancke
authors.forename: H
authors.initials: H
authors.lastname: Beele
authors.forename: S
authors.initials: S
authors.lastname: Monstrey
authors.forename: J M
authors.initials: JM
authors.lastname: Naeyaert
mesh_terms: D002478:Cells, Cultured; D002675:Child, Preschool; D016108:Epidermolysis Bullosa Dystrophica / Q000503:physiopathology / Q000601:surgery*; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D016038:Skin Transplantation / Q000379:methods*; D014182:Transplantation, Autologous / Q000379:methods; D014184:Transplantation, Homologous / Q000379:methods; D014945:Wound Healing / Q000502:physiology