Cell-cycle-dependent phosphorylation of the basal transcription factor RAP74.
In this report, cell-cycle-dependent effects of TFIID on other basal transcription factors were investigated. We purified TFIID fractions from HeLa cells synchronized in the S/G2 phases and in early G1 phase, and show that RAP74 is phosphorylated more highly by the S/G2 phase TFIID fraction than by the early G1 phase TFIID fraction. Further analyses using deletion mutants of RAP74 revealed that amino acid residues 206-256 are phosphorylated by the TFIID fraction. Reconstitution of in vitro transcription activity indicates that the cell-cycle-dependent phosphorylation of RAP74 increases TFIIF transcription activity.
Yonaha
M
M
Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Japan. yonaha@dunn1.path.ox.ac.uk
Tsuchiya
T
T
Yasukochi
Y
Y
D000818:Animals; D002453:Cell Cycle; D016195:G2 Phase; D006367:HeLa Cells; D006801:Humans; D010766:Phosphorylation; D016196:S Phase; D017384:Sequence Deletion; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019473:Transcription Factors, TFII / Q000378:metabolism*
D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C056604:transcription factor TFIIF