Rigid porous polyacrylamide-based monolithic columns containing butyl methacrylate as a separation medium for the rapid hydrophobic interaction chromatography of proteins.
Macroporous poly(acrylamide-co-butyl methacrylate-co-N,N'-methylenebisacrylamide) monoliths containing up to 15% butyl methacrylate units have been prepared by direct polymerization within the confines of HPLC columns. The hydrodynamic and chromatographic properties of these 50 mm x 8 mm I.D. columns-such as back pressure at different flow-rates, effect of percentage of hydrophobic component in the polymerization mixture, effect of salt concentration on the retention of proteins, dynamic loading capacity, and recovery-were determined under conditions typical of hydrophobic interaction chromatography. Using the monolithic column, five proteins were easily separated within only 3 min.
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Cornell University, Department of Chemistry, Baker Laboratory, Ithaca, NY 14853, USA.
Svec
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Fréchet
J M
JM
D000180:Acrylic Resins; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D008689:Methacrylates; D011506:Proteins / Q000302:isolation & purification*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D008689:Methacrylates; D011506:Proteins; D000099166:Polyacrylamides; C016284:butyl methacrylate