title: Expression of matrilysin in vascular endothelial cells adjacent to matrilysin-producing tumors.
abstract: Matrilysin is believed to play important roles in tumor progression and metastasis. In the present study, we analyzed matrilysin-producing cells in various human cancer tissues by immunohistochemistry and in situ hybridization. Tumor cells in colorectal carcinomas, pancreatic carcinomas, transitional-cell carcinomas of the kidney and small-cell lung carcinomas were frequently positive for matrilysin. In addition, we found that endothelial cells of arterioles and venules adjacent to matrilysin-positive tumors expressed matrilysin mRNA and protein. The endothelial cells adjacent to matrilysin-negative tumors and those in normal tissues were negative for matrilysin. Furthermore, analyses by casein zymography, Western blotting and RT-PCR showed that matrilysin was weakly expressed by cultured human umbilical vein endothelial cells. Our results suggest that the expression of matrilysin in vascular endothelial cells and in tumor cells may be regulated by common soluble factors, and that endothelial cell-derived matrilysin may contribute to tumor angiogenesis and tumor metastasis.
authors.affiliation: Department of Pathology, Yokohama City University School of Medicine, Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagashima
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
authors.forename: N
authors.initials: N
authors.lastname: Koshikawa
authors.forename: A
authors.initials: A
authors.lastname: Taki
authors.forename: Y
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: H
authors.initials: H
authors.lastname: Kitamura
authors.forename: K
authors.initials: K
authors.lastname: Misugi
authors.forename: Y
authors.initials: Y
authors.lastname: Kihira
authors.forename: Y
authors.initials: Y
authors.lastname: Matuo
authors.forename: H
authors.initials: H
authors.lastname: Yasumitsu
authors.forename: K
authors.initials: K
authors.lastname: Miyazaki
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D018288:Carcinoma, Small Cell / Q000201:enzymology; D002295:Carcinoma, Transitional Cell / Q000201:enzymology; D002478:Cells, Cultured; D015179:Colorectal Neoplasms / Q000201:enzymology; D004730:Endothelium, Vascular / Q000201:enzymology*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007680:Kidney Neoplasms / Q000201:enzymology; D008175:Lung Neoplasms / Q000201:enzymology; D020783:Matrix Metalloproteinase 7; D008666:Metalloendopeptidases / Q000032:analysis / Q000235:genetics*; D051379:Mice; D009369:Neoplasms / Q000098:blood supply* / Q000201:enzymology*; D010190:Pancreatic Neoplasms / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D013274:Stomach Neoplasms / Q000201:enzymology; D014471:Umbilical Veins
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D008666:Metalloendopeptidases; D020783:Matrix Metalloproteinase 7