title: betagamma-Transducin stimulates hydrolysis and synthesis of phosphatidylinositol 4,5-bisphosphate in bovine rod outer segment membranes.
abstract: T betagamma was shown to stimulate the hydrolysis and synthesis of PtdInsP2 in dark-adapted bovine retinal rod outer segments. In contrast, T alphaGDP blocked the effect of betagamma-transducin. It was also demonstrated that T betagamma was a stimulator of 32P incorporation into PtdInsP2 in ROS. These findings explain the modulating actions of GTP and light on PtdInsP2 hydrolysis and synthesis in ROS. The possible existence of cross-talk between the cGMP and phosphoinositide cascades in retinal rods was discussed.
authors.affiliation: Institute of Photobiology, Academy of Sciences of Belarus, Minsk.
authors.forename: I V
authors.initials: IV
authors.lastname: Grigorjev
authors.forename: A I
authors.initials: AI
authors.lastname: Grits
authors.forename: I D
authors.initials: ID
authors.lastname: Artamonov
authors.forename: L A
authors.initials: LA
authors.lastname: Baranova
authors.forename: I D
authors.initials: ID
authors.lastname: Volotovski
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006868:Hydrolysis; D008027:Light; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000096:biosynthesis*; D012374:Rod Cell Outer Segment / Q000187:drug effects* / Q000378:metabolism; D015293:Transducin / Q000037:antagonists & inhibitors / Q000494:pharmacology*
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D006160:Guanosine Triphosphate; D015293:Transducin