[Analysis of aromatic DNA adducts in inhabitants of Upper Silesia].
Aromatic DNA adducts levels were measured in white blood cells collected from three groups of volunteers: occupationally exposed group (cokery workers), environmentally exposed group (residents from Silesia), control group (inhabitants of Biała Podlaska) using ELISA and 32P-postlabelling methods. The highest level of DNA adducts was observed in occupationally exposed group, slightly lower in the environmentally exposed group and much lower in the control group. Seasonal variations in the adducts levels were found in total white blood cells and in lymphocytes showing that the levels of DNA adducts reflect the present exposure to genotoxic agents in the air. It was also shown using 32P-postlabelling with HPLC that not only adducts with polycyclic aromatic hydrocarbons were found in human DNA.
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Zakład Biologii Molekularnej Centrum Onkologii, Instytut im. Marii Skłodowskiej-Curie, Oddział w Gliwicach.
D000328:Adult; D000393:Air Pollutants / Q000009:adverse effects / Q000032:analysis*; D000818:Animals; D002648:Child; D018849:Controlled Clinical Trials as Topic; D018736:DNA Adducts / Q000032:analysis*; D004781:Environmental Exposure; D004784:Environmental Monitoring; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000737:chemistry*; D016273:Occupational Exposure; D011044:Poland; D011083:Polycyclic Compounds / Q000032:analysis*; D012621:Seasons
D000393:Air Pollutants; D018736:DNA Adducts; D011083:Polycyclic Compounds
Analiza adduktów aromatycznych DNA u mieszkańców Górnego Slaska.