Identification and characterization of the gyrB gene from Treponema pallidum subsp. pallidum.
The nucleotide sequence of a DNA gyrase B subunit gene (gyrB) from Treponema pallidum has been determined. Southern blot analysis of T. pallidum chromosomal DNA indicated that this gene is present as a single copy. The organization of genes flanking the gyrB gene is unique in comparison to that of other bacteria. The gyrB gene encodes a 637 amino acid protein whose deduced sequence has a high degree of homology with type-II topoisomerase ATPase subunits (GyrB and ParE). Five type-II topoisomerase motifs, an ATP-binding site (Walker A), and amino acid residues that putatively interact with ATP, are highly conserved in the T. pallidum GyrB protein.
Stamm
L V
LV
Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599-7400, USA. istamm@email.unc.edu
Greene
S R
SR
Barnes
N Y
NY
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D017124:Conserved Sequence / Q000235:genetics; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D018628:Gene Dosage; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014210:Treponema pallidum / Q000235:genetics*
D000255:Adenosine Triphosphate; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II