Identification of a biological inactive complex form of pokeweed antiviral protein.
Pokeweed antiviral protein (PAP) inactivates both eukaryotic and prokaryotic ribosomes via a specific depurination of rRNA. The sensitivity of pokeweed ribosomes to PAP implies the existence of a mechanism to protect the plant. Using monoclonal antibodies specific to PAP, a protein complex (PAPi) which contained PAP was identified in leaf extract. In this complex, the enzymatic activity of the toxin was strongly inhibited. This protein complex had a pI lower than that of PAP and was separated from free PAP by a preparative native gel electrophoresis. PAPi had an apparent molecular mass of 57 kDa and was dissociated by heating for 5 min at 80 degrees C or by treatment by alkaline or acidic pH or by 7 M urea. The other components involved in the complex remain unknown.
Desvoyes
B
B
Laboratoire de Biochimie et Biologie Moléculaire, UFR Sciences et Techniques, Besançon, France.
Poyet
J L
JL
Schlick
J L
JL
Adami
P
P
Jouvenot
M
M
Dulieu
P
P
D000818:Animals; D000998:Antiviral Agents / Q000032:analysis* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000032:analysis* / Q000494:pharmacology; D010944:Plants / Q000737:chemistry; D011489:Protein Denaturation; D054789:Ribosome Inactivating Proteins, Type 1
D000998:Antiviral Agents; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein