Genetic divergence of Asiatic Bdellocephala (Turbellaria, Tricladida, Paludicola) as revealed by partial 18S rRNA gene sequence comparisons.
Polymerase chain reaction (PCR) and direct sequencing of small ribosomal RNA genes were used for analysis of genetic differences among Asiatic species of freshwater triclad genus Bdellocephala. Representatives of four species and four subspecies of this genus were used to establish homology between nucleotides in the 5'-end portion of small ribosomal RNA gene sequences. Within 552 nucleotide sites of aligned sequences compared, six variable base positions were discovered, dividing Bdellocephala into five different genotypes. Sequence data allow to distinguish two groups of these genotypes. One of them unites species from Kamchatka and Japan, another one unites Baikalian taxa. Agreement between available morphological, cytological and sequence data is discussed.
Kuznedelov
K D
KD
Limnological Institute SB RAN, Irkutsk, Russia.
Timoshkin
O A
OA
Goldman
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E
D000818:Animals; D001208:Asia; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics; D017238:Genes, Helminth / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods; D014419:Turbellaria / Q000145:classification / Q000235:genetics*
D018509:DNA, Helminth; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S