title: Integrated map of the Schizosaccharomyces pombe genome.
abstract: A restriction map of the entire Schizosaccharomyces pombe genome was constructed using two restriction enzymes (BamHI and PstI) that recognize 6 bp. The restriction map contains 420 minimally overlapping clones (miniset) and has 22 gaps. We located 126 genes, marker fragments of DNA (NotI and SfiI linking clones), and 36 transposable elements by hybridization to unique restriction fragments.
authors.affiliation: Department of Medical Biochemistry and Southern Alberta Cancer Research Center, University of Calgary HSC, 3330 Hospital Dr. N.W., Calgary, Alberta, Canada T2N 4N1. igarkavt@acs.ucalgary.ca
authors.forename: I
authors.initials: I
authors.lastname: Garkavtsev
authors.forename: T
authors.initials: T
authors.lastname: Mizukami
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D005819:Genetic Markers; D016681:Genome, Fungal; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping / Q000379:methods*; D012568:Schizosaccharomyces / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D004271:DNA, Fungal; D005819:Genetic Markers