Increase of eosinophilic cell population in bone marrow of rats by immunization with Ascaris suum antigen.
Immunization of rats with the antigen, Ascaris suum extract, increased the number of peripheral eosinophils. Analysis by Western blot and reverse transcription-polymerase chain reaction revealed that the levels of major basic protein and its mRNA in the bone marrow were also increased, suggesting that eosinophilic cell population in the bone marrow is increased by the immunization. These findings indicate that immunization with this antigen stimulates differentiation of progenitor cells to eosinophils in the bone marrow, and induces blood eosinophilia.
Nittoh
T
T
Department of Pathophysiological Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
Arii
M
M
Suzuki
R
R
Kitoh
A
A
Watanabe
M
M
Mue
S
S
Ohuchi
K
K
D000818:Animals; D000947:Antigens, Helminth / Q000008:administration & dosage*; D017165:Ascaris suum / Q000276:immunology*; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology* / Q000378:metabolism; D001854:Bone Marrow Cells; D002452:Cell Count; D017931:DNA Primers / Q000235:genetics; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006410:Hematopoiesis / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D007114:Immunization; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012260:Ribonucleases
D000947:Antigens, Helminth; D001798:Blood Proteins; D017931:DNA Primers; D047091:Eosinophil Granule Proteins; D012333:RNA, Messenger; D012260:Ribonucleases