Differences in phycoerythrin- or fluorescein-isothiocyanate conjugated 8G12 on CD34+ cell evaluation.
To evaluate the equivalence of 8G12 conjugated with either phycoerythrin (PE) or fluorescein isothiocyanate (FITC), duplicate fluorochrome-8G12 labelling was carried out in 229 samples. Additionally, 53 samples were simultaneously immunostained with FITC-8G12 and PE-8G12. Significantly higher values (p < 0.001) were observed in PE-CD34+ cells when compared with FITC-CD34+ cells both in duplicate and simultaneous analysis. Our data suggest that the choice of fluorochrome is relevant in the measurement of CD34+ cells with 8G12.
Ortuño
F
F
Unit of Hematology and Hemotherapy, School of Medicine, Hospital General Universitario, Murcia, Spain.
Ferrer
F
F
Lozano
M L
ML
Heras
I
I
Moraleda
J M
JM
Vicente
V
V
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D018952:Antigens, CD34 / Q000032:analysis* / Q000276:immunology; D000970:Antineoplastic Agents / Q000494:pharmacology; D002469:Cell Separation; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D005456:Fluorescent Dyes; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000187:drug effects; D006801:Humans; D007937:Leukapheresis; D007958:Leukocyte Count / Q000187:drug effects; D010799:Phycoerythrin; D011994:Recombinant Proteins / Q000494:pharmacology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D010799:Phycoerythrin; D016179:Granulocyte Colony-Stimulating Factor; D016650:Fluorescein-5-isothiocyanate