title: Purification, sequence determination and synthesis of seminal plasma peptides and synthesis of some of their analogues.
abstract: Three peptides were isolated from bovine seminal plasma and purified to homogeneity. The amino acid sequences, as determined by FAB mass spectrometry, are the following: pGlu-Ala-Glu-Ser-Asn-OH, pGlu-Ala-Glu-Ser(PO3H2-Asn-OH and pGlu-Val-Gly-Glu-Ser-Glu-Asn-OH. These three peptides and some of their analogues were synthesized using liquid- and solid-phase techniques. The pentapeptide pGlu-Ala-Glu- Ser-Asn-OH showed a remarkable affinity for kinase NII and a strong inhibiting activity in DNA transcription. These findings support the hypothesis that phosphorylated acidic domains of nuclear non-histone proteins could bind to DNA, thereby controlling transcription.
authors.affiliation: Department of Organic and Industrial Chemistry, University of Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Francescato
authors.forename: G
authors.initials: G
authors.lastname: Lugaro
authors.forename: G I
authors.initials: GI
authors.lastname: Gianfranceschi
authors.forename: L
authors.initials: L
authors.lastname: De Angelis
authors.forename: F
authors.initials: F
authors.lastname: Chillemi
mesh_terms: D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D013058:Mass Spectrometry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification*; D010766:Phosphorylation; D029584:Prostatic Secretory Proteins; D011494:Protein Kinases / Q000378:metabolism; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification*; D012313:RNA / Q000378:metabolism; D051381:Rats; D012265:Ribonucleotides / Q000378:metabolism; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D017421:Sequence Analysis; D012996:Solutions
chemical_list: D010455:Peptides; D029584:Prostatic Secretory Proteins; D011506:Proteins; D012265:Ribonucleotides; D029589:Seminal Plasma Proteins; D012996:Solutions; C043076:beta-microseminoprotein; D012313:RNA; D011494:Protein Kinases; C026813:protein kinase NII