Tetracycline-dependent activation of an upstream promoter reveals transcriptional interference between tandem genes within T-DNA in tomato.
Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also demonstrated in fruit. Activation of the Triple-Op promoter reduced expression of the downstream hygromycin phosphotransferase II (hptII) gene. Transcriptional interference was therefore observed between tandem genes within T-DNA. Such interference may occur in other T-DNAs, but would not be readily observed without an upstream promoter with controllable activity.
Thompson
A J
AJ
Department of Plant Genetics and Biotechnology, Horticulture Research International, Wellesbourne, Warwickshire, UK.
Myatt
S C
SC
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics; D013752:Tetracycline / Q000494:pharmacology; D014158:Transcription, Genetic
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012097:Repressor Proteins; C036483:T-DNA; C034811:tetracycline resistance-encoding transposon repressor protein; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase; D005966:Glucuronidase; D013752:Tetracycline