Water immiscible solvent based immunoassay.
A competitive monoclonal antibody-based immunoassay which quantifies a hydrophobic hapten (Rx) in water immiscible solvents, obviating the need of a pre-extraction step, has been developed. Approximately linear dose response profiles of analyte, over the range 1-20 ugml-1 in the hydrophobic solvents, hexane, toluene and xylene were obtained. UV spectrophotometric analyses of Rx dosed hexane confirm the phenomenon of antibody-mediated transfer of analyte from the organic to the aqueous milieu. Preliminary data on the effect of water immiscible solvents on the immunoreactivity of a monoclonal antibody in free solution are presented. The potential industrial applications of water immiscible solvent based immunoassays are discussed.
Aston
J P
JP
Molecular Light Technology Research Ltd., Cardiff.
Hitchings
E J
EJ
Ball
R L
RL
Weeks
I
I
Woodhead
J S
JS
D000911:Antibodies, Monoclonal / Q000737:chemistry; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006241:Haptens / Q000276:immunology; D006801:Humans; D012709:Serum Albumin / Q000737:chemistry; D012995:Solubility; D012997:Solvents; D014466:Ultraviolet Rays; D014867:Water
D000911:Antibodies, Monoclonal; D006241:Haptens; D012709:Serum Albumin; D012997:Solvents; D014867:Water