title: A renin-like enzyme in the leech Theromyzon tessulatum.
abstract: We report on the biochemical isolation and characterization of a 32 kDa aspartyl protease from the leech Theromyzon tessulatum. Following a three step purification (gel permeation chromatography, pepstatin A-sepharose affinity column separation followed by reversed-phase HPLC) a renin-like enzyme was purified to homogeneity. The first 124 amino acid residues of the N-terminal part of the purified S-pyridylethylated leech renin exhibits a 26.5-35.5% sequence identity with that of mammals. The 20-81 region of leech renin exhibits a 80% sequence homology with the 175-232 region in mammals. This highly conserved region, which is also found in all aspartic proteases, possesses the aspartyl catalytic residue (D11TGSS). Leech renin hydrolyses at neutral pH and at 37 degrees C the Leu10-Leu11 bond of synthetic porcine angiotensinogen tetradecapeptide yielding the angiotensin I and the Leu11-Val12-Tyr13-Ser14 peptides, with a specific activity of 115 microg AI/min/mg (K[M] 22 microM; K[cat], 2.7). This hydrolysis is inhibited by pepstatin A (IC50: 4.6 microM). Moreover, this enzyme is found on a multiple hormone precursor of 19 kDa which exhibits a specific activity of 850 pmol AI/min/mg of renin. This is the first biochemical characterization of a renin-like enzyme in invertebrates and non-mammalian vertebrates.
authors.affiliation: Centre de Biologie Cellulaire, Laboratoire de Phylogénie moléculaire des Annélides, Université des Sciences et Technologies de Lille, Villeneuve d' Ascq, France. salzet@pop.univ-lille1.fr
authors.forename: M
authors.initials: M
authors.lastname: Salzet
authors.forename: G
authors.initials: G
authors.lastname: Stefano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D007700:Kinetics; D007865:Leeches / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D012083:Renin / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D012083:Renin