[Separation and identification of beta-carotene and its cis isomers by high pressure liquid chromatography (HPLC)].
The separation and identification by HPLC of the cis isomers of beta-carotene was studied. A 1.26 mg/ml beta-carotene solution previously isomerized with iodine as a catalyst, was eluted with 2% acetone in hexane, from a Ca(OH)2 chromatographic column in three bands. The fractions were identified by spectrophotometry and the retention times of 2.05, 2.4 and 2.8 min for the 13 cis, all-trans, and 9 cis beta-carotene isomers, determined by HPLC, with 1% acetone in hexane as movil phase. 22.13 mg % of all-trans beta-carotene were found in a sample of canned carrots. It is recommended the analyses of a greater number of samples, the determination of the method's sensitivity, reproductibility, and the use of a standard of reference of a response factor for calculations.
Carrillo de Padilla
F
F
Cátedra de Análisis de Alimentos, Facultad de Farmacia UCV, Caracas, Venezuela.
D002851:Chromatography, High Pressure Liquid; D018552:Daucus carota / Q000737:chemistry; D019207:beta Carotene / Q000302:isolation & purification*
D019207:beta Carotene
Separación e identificación del beta-caroteno y sus isómeros cis por cromatografía líquida de alta resolución (HPLC).