Computer simulations to identify in polyproteins of FMDV OK1 and A12 strains putative nonapeptides with amino acid motifs for binding to BoLA class I A11 and A20 haplotype molecules.
The computer program "Findpatterns" was used to search FMDV- (OK1 and A12 strains) coded structural and nonstructural proteins for the availability of putative proteasome-generated nonapeptides with motifs reported for BoLA class I A11 and A20 haplotypes. These BoLA class I A11 and A20 nonapeptide motifs are identical to motifs of nonapeptides that interact with the peptide binding grooves of HLA class I B35 and B27 haplotypes, respectively. The computer findpattern program was used to analyze the FMDV-coded polyproteins for proteolytically cleavable nonapeptides with motifs for binding to the peptide binding grooves of BoLA class I A11 or 20 haplotypes. The computer simulations revealed that FMDV-infected cells (keratinocytes and antigen presenting cells. e.g., dendritic Langerhans cells in bovines) may be able to present viral nonapeptides to CD8+ cytolytic T cells (CTLs) in a BoLA-restricted manner. The role of the cellular arm of the immune response in the protection of bovines against FMDV is not known. Thus, the present computer analysis may encourage further experiments to develop a new generation of FMDV nonapeptide vaccines to stimulate the anti-FMDV cytolytic T cell response in bovine so this would complement the humoral immune response achieved by immunization with the inactivated virus vaccine.
Becker
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Department of Molecular Virology, Faculty of Medicine, Hebrew University of Jerusalem, Israel.
D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000235:genetics* / Q000276:immunology*; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D003198:Computer Simulation; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
D015395:Histocompatibility Antigens Class I; D009842:Oligopeptides; D014764:Viral Proteins