Evolution of transcription-regulating proteins: caveat lector!
The paper of Hawkins et al. [Gene 146 (1994) 145-158] reports incorrect descriptions of mutant phenotypes, omits mention of the absence of a highly relevant glutamine-binding site and contains sequence alignments which might mislead the reader. Extensive sequence analysis reveals as untenable a central hypothesis of the paper concerning a possible evolutionary relationship between anthranilate synthetases and the transcription factors mediating nitrogen metabolite repression in fungi.
Arst
H N
HN
Holden
D W
DW
Caddick
M X
MX
D000878:Anthranilate Synthase / Q000235:genetics; D016477:Artifacts; D001233:Aspergillus nidulans / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D019143:Evolution, Molecular; D005656:Fungal Proteins; D015966:Gene Expression Regulation, Fungal; D008957:Models, Genetic; D009492:Neurospora crassa / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods; D017421:Sequence Analysis; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C068100:NIT2 protein, Neurospora crassa; D014157:Transcription Factors; D000878:Anthranilate Synthase