Procedure for red blood cell labelling with 99mTc-HMPAO. Methodology and quality control.
We present here results on the labelling of red blood cells with 99mTc-HMPAO as an alternative method to the usual in vitro technique. Anticoagulant agents, the labelling medium with plasma, and the lapse of time between 99mTc-HMPAO preparation and labelling are the main factors which affect the efficiency of the procedure. A 93.9 +/- 2.3% labelling yield was obtained with freshly prepared 99mTc-HMPAO. In vitro (tracer elution of 4.3 +/- 1.2% at 60 mins) and in vivo (percentage of plasma activity at 60 mins, 7.8 +/- 2.8%) stability of the label, as well as image quality, qualify 99mTc-HMPAO labelled red blood cells as a suitable agent for clinical use.
Bassa
P
P
Nuclear Medicine Service, Hospital Clinic I Provincial de Barcelona, Spain.
García Garzón
J R
JR
Piera
C
C
Pavía
A
A
Minoves
M
M
Moragas
M
M
Pavía
J
J
Lomeña
F
F
Setoain
J
J
D004912:Erythrocytes; D006321:Heart / Q000000981:diagnostic imaging; D006801:Humans; D015609:Organotechnetium Compounds; D010091:Oximes; D011786:Quality Control; D011877:Radionuclide Imaging; D019690:Technetium Tc 99m Exametazime
D015609:Organotechnetium Compounds; D010091:Oximes; D019690:Technetium Tc 99m Exametazime