Glutathione S-transferase pi-class as a tumour marker in lingual preneoplastic and neoplastic lesions of rats and humans.
Immunocytochemical expression of the pi class glutathione S-transferase (GST) was investigated in preneoplastic and neoplastic lingual lesions in a 4-nitro-quinoline 1-oxide (4NQO)-induced rat genetic model [Wistar/Furth rats (WF) and Dark-Agouti rats (DA)] and in human surgical material [fibrous polyp, mild to moderate dysplasia, severe dysplasia, carcinoma in situ (CIS), squamous cell carcinoma (SCC)]. Two polyclonal antibodies raised against rat (GST-P) and human (GST-pi) antigens were used. In the rat model, DA and WF rats showed contrasting susceptibility to 4NQO, DA rats having a much higher tumour incidence and a significantly shorter survival time than WF rats. While the established lingual SCC in DA and WF rats all expressed GST-P, the number of GST-P+ foci in the preneoplastic lingual epithelium was significantly higher in DA (14.5 +/- 6.5) than in WF rats (5.5 +/- 2.6; P < 0.0001). In contrast, GST-pi epithelial staining in human specimens was more variable and the results overlapped in different groups. More frequent nuclear and/or basal cell staining was detected in severe dysplasia, CIS and SCC than in benign and mild to moderate dysplastic lesions. Although the pi class GST may be a useful marker for rat lingual carcinogenesis, its value in clinical applications is unclear. GST-pi staining patterns and their distribution may be helpful in identifying high-risk lingual lesions in humans.
Li
T J
TJ
Department of Oral Pathology, Kagoshima University Dental School, Japan.
Hirayama
Y
Y
Kitano
M
M
D015112:4-Nitroquinoline-1-oxide / Q000009:adverse effects; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D002273:Carcinogens / Q000009:adverse effects; D002278:Carcinoma in Situ / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D008297:Male; D011230:Precancerous Conditions / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D051381:Rats; D011920:Rats, Inbred WF; D014062:Tongue Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology
D014408:Biomarkers, Tumor; D002273:Carcinogens; D015112:4-Nitroquinoline-1-oxide; D005982:Glutathione Transferase