H+, K(+)-ATPASE in the kidney: localization and function in the nephron.
In the past few years, many studies aimed at characterizing the localisation, the molecular nature, and the pharmacological and functional properties of renal H+, K(+)-ATPases. These studies included: (1) molecular cloning (by homology with other known P-type K(+)-ATPases) and expression of H+, K(+)-ATPase alpha-subunits from amphibian and mammalian epithelia; (2) kinetic and pharmacological characterization of K(+)-ATPase activities different from Na+, K(+)-ATPase in single segments of rat nephron, and (3) analysis of K+ and/or H+ transport in isolated collecting ducts from rat or rabbit kidney. The present paper summarizes these data with a special emphasis on those which demonstrate the existence along the mammalian nephron of several forms of H+, K(+)-ATPase displaying distinct biochemical, pharmacological and functional properties.
Doucet
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Laboratoire de Biologie Intégrée des Cellules Rénales, CNRS URA 1859, SBCe, CEA Saclay, Gif-sur-Yvette, France.
D000663:Amphibians; D000818:Animals; D003001:Cloning, Molecular; D004848:Epithelium / Q000201:enzymology; D017506:H(+)-K(+)-Exchanging ATPase / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D008322:Mammals; D009399:Nephrons / Q000201:enzymology*; D011817:Rabbits; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D011994:Recombinant Proteins; D017506:H(+)-K(+)-Exchanging ATPase; D000254:Sodium-Potassium-Exchanging ATPase