Correlation between direct binding ability of synthetic T. gondii SAG1 peptides to HLA-A2 measured by a sensor for surface plasmon resonance and antigenicity of the peptides for T. gondii-infected cell-specific CTL.
Toxoplasma gondii (T. gondii) -infected B lymphoma cells present T. gondii antigens in the context of major histocompatibility complex molecules to T. gondii-specific CD8+ cytotoxic T cells (CTL). HLA-A2 molecules of T. gondii-infected human cells have been shown to be used in presenting T. gondii antigens to CD8+ CTL. SAG1, one of the major antigenic molecules of T. gondii, is an antigen for T. gondii-specific CTL, and represents a possible basis for vaccines. The direct binding of nonamer SAG1 peptides to HLA-A2 was assayed here using an automated biosensor system with a sensor for surface plasmon resonance detection. The antigenicity of synthetic SAG1 peptides to T. gondii-specific CD8+ CTL also was assayed. The present study found a high correlation between the binding ability of synthetic SAG1 peptides to HLA-A2 and the antigenicity of peptides to T. gondii-infected cell-specific CD8+ CTL.
Yano
A
A
Department of Parasitology, Chiba University School of Medicine, Chuo-ku, Japan. yano@med.m.chiba-u.ac.jp
Aosai
F
F
Yang
T H
TH
He
N
N
Mun
H S
HS
Liu
H
H
Inoko
H
H
Norose
K
K
D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D018604:Epitope Mapping; D015789:HLA-A2 Antigen / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D015800:Protozoan Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*; D014122:Toxoplasma / Q000276:immunology*; D014614:Vaccines, Synthetic
D000953:Antigens, Protozoan; D015789:HLA-A2 Antigen; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma; D014614:Vaccines, Synthetic