Beta 2-adrenergic activation enhances interleukin-8 production by human monocytes.
Interleukin-8 (IL-8) is an important cytokine in inflammatory processes by functioning as a chemoattractant and as an activator of oxygen metabolism. In the present study we demonstrate that beta 2-adrenergic agonists potentiate the lipopolysaccharide (LPS) and IL-1 induced production of IL-8 by human monocytes. In addition, beta 2-adrenergic activation enhances IL-8 release and mRNA expression for IL-8 in the human monocytic cell line U937. beta 2-adrenergic activation of these cells also results in enhanced production of the anti-inflammatory cytokine IL-10. However, IL-10 is not involved in the regulation of IL-8 production. The effect of the beta 2-adrenergic agonist on IL-8 production is presumably mediated via increased cAMP formation, since it can be mimicked by the cAMP analogue dibutyryl-cAMP (db-cAMP). We conclude that enhancement of IL-8 production is one of the pathways via which beta 2-adrenergic agonists such as catecholamines can influence inflammatory responses.
Kavelaars
A
A
Department of Immunology, University Hospital for Children and Youth "Het Wilhelmina Kinderziekenhuis", Utrecht, The Netherlands. a.kavelaars@wkz.ruu.nl
van de Pol
M
M
Zijlstra
J
J
Heijnen
C J
CJ
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D018343:Receptors, Adrenergic, beta-2 / Q000187:drug effects / Q000378:metabolism*; D013726:Terbutaline / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000318:Adrenergic beta-Agonists; D016209:Interleukin-8; D012333:RNA, Messenger; D018343:Receptors, Adrenergic, beta-2; D016753:Interleukin-10; D003994:Bucladesine; D013726:Terbutaline