title: Kinetics of the thermal inactivation of alkaline phosphatase from green crab (Scylla serrata).
abstract: The kinetics of thermal inactivation of alkaline phosphatase from green crab (Scylla Serrata) has been studied using the kinetic method relating to the substrate reaction during irreversible inhibition of enzyme activity previously described by Tsou. The results show that the thermal inactivation of the enzyme is an irreversible reaction. Comparison of the microscopic rate constants for thermal inactivation of free enzyme and the enzyme-substrate complex shows that the presence of substrate has a certain protective effect against thermal inactivation.
authors.affiliation: Department of Biology, Xiamen University, China.
authors.forename: Q X
authors.initials: QX
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: S X
authors.initials: SX
authors.lastname: Yan
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: H M
authors.initials: HM
authors.lastname: Zhou
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D001665:Binding Sites; D003386:Brachyura; D002851:Chromatography, High Pressure Liquid; D006358:Hot Temperature; D007700:Kinetics; D008670:Metals / Q000378:metabolism
chemical_list: D008670:Metals; D000469:Alkaline Phosphatase