The osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen, which promoted cell-attachment of osteoblasts.
In this study, we purified C-terminal propeptide of type I collagen (PICP) from the conditioned medium of osteoblastic MC3T3-E1 cells by chromatographic and Agarose gel extraction procedures. PICP was confirmed to be present in bone by Western blotting using a specific antibody, and was proved to be synthesized by osteoblasts with metabolic labeling. PICP promoted cell-attachment of osteoblastic MC3T3-E1 cells. We conclude that PICP is synthesized by osteoblasts and stored in bone, and that it plays a role in the maintenance of bone cells on bone matrix.
Mizuno
M
M
Department of Biochemistry, School of Dentistry, Hokkaido University, Saporro, Japan.
Kitafima
T
T
Tomita
M
M
Kuboki
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D011347:Procollagen / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D014316:Tritium
D019485:Bone Morphogenetic Proteins; D003470:Culture Media; D010446:Peptide Fragments; D011347:Procollagen; C042813:procollagen type I carboxy terminal peptide; D014316:Tritium