From gel filtration to adsorptive size exclusion.
Adsorption and size exclusion in starch and cross-linked dextran were phenomena discovered in Uppsala in the 1950s [Porath (1979), Biochem. Soc. Trans. 7, 1197; Porath (1981), Current Content 19, 21; Porath (1981), J. Chromatogr. 218, 241; Janson (1987), Chromatographia 23, 361; Laurent (1993), J. Chromatogr. 633, 1]. These discoveries were the background to the development of a variety of affinity chromatographic methods. At present attempts are being made to combine size exclusion chromatography (SEC) with adsorption into a single operation that we call adsorptive SEC (AdSEC).
Porath
J
J
Biochemical Separation Centre, University of Uppsala, BMC, Sweden.
D000327:Adsorption; D002845:Chromatography / Q000379:methods* / Q000639:trends*; D011506:Proteins / Q000032:analysis*
D011506:Proteins