Direct analysis of protein mixtures by tandem mass spectrometry.
Methods to identify proteins contained in mixtures are described. The approach uses microcolumn liquid chromatography and automated tandem mass spectrometry in conjunction with protein and nucleotide database searching algorithms. This approach is applied to the identification of proteins obtained by immunoprecipitation reactions, interaction with a GST protein fusion products and interaction with a macromolecular complex.
Yates
J R
JR
Department of Molecular Biotechnology, University of Washington, Seattle 98195-7730, USA. jyates@u.washington.edu
McCormack
A L
AL
Schieltz
D
D
Carmack
E
E
Link
A
A
D002853:Chromatography, Liquid / Q000379:methods; D016208:Databases, Factual; D046911:Macromolecular Substances; D013058:Mass Spectrometry / Q000379:methods*; D011233:Precipitin Tests; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D046911:Macromolecular Substances; D011506:Proteins