Effect of anti-apoptotic genes and peptide inhibitors on cytoplasmic acidification during apoptosis.
Cytoplasmic acidification has been shown to occur during the apoptotic process of cell death although its relation with other events in the process are not yet clear. AK-5 tumor cells have been shown to undergo apoptosis upon treatment with stimuli like dexamethasone (1 microM) or with serum from animals that reject AK-5 tumor. The current study was designed to measure the extent of cytoplasmic acidification during apoptosis in AK-5 cells and to study the effect of antiapopoptic genes and peptide inhibitors on cytoplasmic acidification. Our results show that AK-5 cells when triggered into apoptosis show intracellular acidification by about 0.2 pH units and this is prevented when cells are treated with peptide inhibitors. In addition cytoplasmic acidification does not occur when AK-5 cells are transfected with anti-apoptotic genes Nedd-2 A.S, Crm A or bcl-2 which inhibit apoptosis.
Pardhasaradhi
B V
BV
Centre for Cellular and Molecular Biology, Hyderabad, India.
Khar
A
A
Srinivas
U K
UK
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D053143:Caspase 2; D020169:Caspases; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D003593:Cytoplasm; D003907:Dexamethasone / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009842:Oligopeptides / Q000494:pharmacology*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014764:Viral Proteins
D000590:Amino Acid Chloromethyl Ketones; D015853:Cysteine Proteinase Inhibitors; C098738:N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone; D009842:Oligopeptides; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D015843:Serpins; D014764:Viral Proteins; C099158:acetyl-aspartyl-glutamyl-valyl-aspartal; D003907:Dexamethasone; C074641:interleukin-1beta-converting enzyme inhibitor; D053143:Caspase 2; D020169:Caspases; D003546:Cysteine Endopeptidases