Heparin in calcification prevention of porcine pericardial bioprostheses.
Calcific degeneration is the main cause of failure of glutaraldehyde-treated xenograft heart valve substitutes implanted in humans. Coupling of heparin through an intermediate surface-bound substrate containing amino groups showed complete prevention of calcification of glutaraldehyde-treated porcine pericardium implanted subdermally in weanling rats for 5 months (heparin bonded pericardium: calcium, 0.625 +/- 0.24 mg g(-1); glutaraldehyde-only-treated pericardium: calcium, 228.32 +/- 37.39 mg g(-1); P < 0.0001). Conceivably, inactivation of unpaired aldehyde moieties present in bioprostheses after exposure to glutaraldehyde by amino compounds followed by blocking the potential binding sites of the graft with a surface modifying agent like heparin would be the key steps in the prevention of calcification and degeneration of glutaraldehyde-treated biological tissue grafts.
Chanda
J
J
Department of Cardiovascular Surgery, Akita University School of Medicine, Japan.
Kuribayashi
R
R
Abe
T
T
D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001672:Biocompatible Materials / Q000378:metabolism; D001705:Bioprosthesis / Q000592:standards*; D002114:Calcinosis / Q000517:prevention & control*; D002118:Calcium / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism / Q000633:toxicity; D005976:Glutaral / Q000378:metabolism / Q000633:toxicity; D006349:Heart Valve Diseases / Q000517:prevention & control; D006350:Heart Valve Prosthesis / Q000592:standards*; D006493:Heparin / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D010496:Pericardium / Q000473:pathology*; D011475:Prosthesis Failure; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D013552:Swine
D000925:Anticoagulants; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D006493:Heparin; D002118:Calcium; D005976:Glutaral