Reconstitution of functional eukaryotic ribosomes from Dictyostelium discoideum ribosomal proteins and RNA.
40 and 60 S ribosomal subunits have been reconstituted in vitro from purified ribosomal RNA and ribosomal proteins of Dictyostelium discoideum. The functionality of the reconstituted ribosomes was demonstrated in in vitro mRNA-directed protein synthesis. The reassembly proceeded well with immature precursors of ribosomal RNA but poorly if at all with mature cytoplasmic RNA species. Reassembly also required a preparation of small nuclear RNA(s), acting as morphopoietic factor(s).
Mangiarotti
G
G
Department of Clinical and Biological Sciences, University of Turin, Ospedale San Luigi Gonzaga, Regione Gonzole 10, 10043 Orbassano-Torino, Italy.
Chiaberge
S
S
D000818:Animals; D002478:Cells, Cultured; D004023:Dictyostelium; D012322:RNA Precursors / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016053:RNA, Protozoan / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism; D012340:RNA, Ribosomal, 5.8S / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D012322:RNA Precursors; D012331:RNA, Fungal; D012333:RNA, Messenger; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; D012342:RNA, Small Nuclear; D012269:Ribosomal Proteins