title: Phagocytosis of nonopsonized Cryptococcus neoformans by swine microglia involves CD14 receptors.
abstract: The interaction of the opportunistic fungus Cryptococcus neoformans with swine microglia was studied in vitro in the presence and absence of anti-CD14 monoclonal antibodies. In the absence of anti-CD14 antibodies, 36% of microglia had phagocytized nonopsonized, encapsulated cryptococci after 2 hr incubation (effector-to-target ratio, 1:50). Preincubation of microglia with anti-CD14 antibodies resulted in a 63% reduction of phagocytosis. These findings suggest that CD14 receptors facilitate uptake of nonopsonized C, neoformans by resident macrophages within the brain.
authors.affiliation: Department of Medicine, University Hospital Utrecht, The Netherlands.
authors.forename: M M
authors.initials: MM
authors.lastname: Lipovsky
authors.forename: G
authors.initials: G
authors.lastname: Gekker
authors.forename: W R
authors.initials: WR
authors.lastname: Anderson
authors.forename: T W
authors.initials: TW
authors.lastname: Molitor
authors.forename: P K
authors.initials: PK
authors.lastname: Peterson
authors.forename: A I
authors.initials: AI
authors.lastname: Hoepelman
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000494:pharmacology; D001667:Binding, Competitive; D003455:Cryptococcus neoformans / Q000276:immunology* / Q000378:metabolism; D018950:Lipopolysaccharide Receptors; D017628:Microglia / Q000276:immunology* / Q000382:microbiology*; D009895:Opsonin Proteins / Q000378:metabolism*; D010587:Phagocytosis / Q000276:immunology; D011971:Receptors, Immunologic / Q000502:physiology*; D013552:Swine
chemical_list: D000908:Antibodies, Fungal; D018950:Lipopolysaccharide Receptors; D009895:Opsonin Proteins; D011971:Receptors, Immunologic