title: Expression of bone morphogenetic proteins of human neoplastic epithelial cells.
abstract: Bone morphogenetic proteins (BMPs) are crucial factors of osteogenesis. We investigated the expressions of BMP subtypes in human salivary adenocarcinoma cell line (HSG-S8), tongue squamous cell (HSC-4) and gingival squamous cell (Ca9-22) carcinoma cell lines, gastric poorly differentiated adenocarcinoma cell (MNK45) and signet ring cell (KATOIII) carcinoma cell lines, rectal adenocarcinoma (RCM-1, RCM-2, and RCM-3), and thyroid (8505C) and bladder (T24) carcinoma cell lines by reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR disclosed that BMP-1 was expressed in all cell lines examined, and BMP-2 was amplified in almost all cells except MKN45. Two squamous cell carcinomas, HSC-4 and Ca9-22, and KATOIII expressed only BMP-1 and BMP-2. MKN45 did not express BMP-2, but expressed BMP-7 and weakly BMP-4 and BMP-5. In addition to the expression BMP-7, and HSG-S8 expressed BMP-6. These findings indicated that the neoplastic epithelial cells possessed a rather great potency to express BMP mRNAs. On the other hand, among these carcinoma cells, HSG-S8 solely induced bone in nude mouse tumors, and HSC-4 and KATOIII contained many calcified masses in tumors while the rest did not induce either.
authors.affiliation: Department of Oral Pathology, School of Dentistry, Iwate Medical University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hatakeyama
authors.forename: Y H
authors.initials: YH
authors.lastname: Gao
authors.forename: Y
authors.initials: Y
authors.lastname: Ohara-Nemoto
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: M
authors.initials: M
authors.lastname: Satoh
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis* / Q000235:genetics; D002114:Calcinosis / Q000209:etiology; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000637:transplantation
chemical_list: D019485:Bone Morphogenetic Proteins; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins