title: Butylated hydroxytoluene exposure is necessary to induce lung tumors in BALB mice treated with 3-methylcholanthrene.
abstract: Chronic butylated hydroxytoluene (BHT) treatment after a single administration of a carcinogen increases lung tumor multiplicity in some inbred strains of mice. We report that BALB/cOla and BALB/cByJ mice given a low dose (10 microg/g of body weight) of 3-methylcholanthrene (MCA) develop no lung tumors unless this is followed by chronic BHT exposure. Slightly higher MCA doses (15 and 25 microg/g) induce low lung tumor multiplicities (0.6 and 1.9 tumors/mouse, respectively) that are increased 12-26-fold by chronic BHT administration. This low-dose MCA/BHT model in BALB mice will facilitate the identification of genes regulating susceptibility to lung tumor promotion and pulmonary chemopreventative agents that act at a postinitiation site.
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, Colorado Cancer Center, University of Colorado Health Sciences Center, Denver 80262, USA. al.malkinson@uchsc.edu
authors.forename: A M
authors.initials: AM
authors.lastname: Malkinson
authors.forename: K M
authors.initials: KM
authors.lastname: Koski
authors.forename: W A
authors.initials: WA
authors.lastname: Evans
authors.forename: M F
authors.initials: MF
authors.lastname: Festing
mesh_terms: D000818:Animals; D002084:Butylated Hydroxytoluene / Q000633:toxicity*; D005260:Female; D008175:Lung Neoplasms / Q000139:chemically induced*; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D002084:Butylated Hydroxytoluene; D008748:Methylcholanthrene