title: The complete mature bovine prion protein highly expressed in Escherichia coli: biochemical and structural studies.
abstract: According to the 'protein only' hypothesis, modification of the 3-dimensional fold of the constituent cellular protein, PrP(C), into the disease-associated isoform, PrP(Sc), is the cause of neurodegenerative diseases in animals and humans. Here we describe the high-level synthesis in Escherichia coli, and purification in the monomeric form, of a histidine-tagged full-length mature PrP (25-249) of bovine brain, termed His-PrP. Based on biochemical and spectroscopic data, His-PrP displays characteristics expected for the PrP(C) isoform. The reported expression system should allow the production of quantities of bovine PrP(C) sufficient to permit 3-dimensional structure determinations.
authors.affiliation: Dipartimento di Chimica Biologica, Centro CNR dello Studio delle Biomembrane, Università di Padova, Padua, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Negro
authors.forename: V
authors.initials: V
authors.lastname: De Filippis
authors.forename: S D
authors.initials: SD
authors.lastname: Skaper
authors.forename: P
authors.initials: P
authors.lastname: James
authors.forename: M C
authors.initials: MC
authors.lastname: Sorgato
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D011328:Prions / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011328:Prions; D019286:Endopeptidase K