Inducible antisense inhibition of erbB-2 expression reduces anchorage independent growth of ovarian carcinoma cells.
We have investigated the effect of c-erbB-2 expression on the growth of ovarian carcinoma cell lines using antisense methodology. A 1.5 kb fragment of c-erbB-2 cDNA was cloned in an antisense and sense orientation into an IPTG inducible vector. These vectors were stably transfected into two ovarian carcinoma cell lines, one of which (NIH:OVCAR-8) grew well in soft agar. Inhibition of expression of endogenous c-erbB-2 protein was detected by immunoprecipitation and Western blot in both of the induced transfectants with the antisense construct. Although growth in monolayer culture was unaffected, NIH:OVCAR-8 cells transfected with the antisense construct and induced with IPTG lost their ability to form colonies in soft agar. Consequently, endogenous expression of c-erbB-2 modulates anchorage-independent growth of NIH:OVCAR-8.
Pegues
J C
JC
Division of Cytokine Biology, Center for Biologics Research and Evaluation, Food and Drug Administration, FDA/CBER, Bethesda, MD 20892, USA.
Stromberg
K
K
D002277:Carcinoma / Q000235:genetics / Q000473:pathology*; D002448:Cell Adhesion; D002455:Cell Division; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018734:Genes, erbB-2; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*
D016372:RNA, Antisense; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases