title: Cell-free ubiquitination of cell cycle regulators in budding yeast extracts.
authors.affiliation: California Institute of Technology, Pasadena 91125, USA.
authors.forename: R
authors.initials: R
authors.lastname: Verma
authors.forename: Y
authors.initials: Y
authors.lastname: Chi
authors.forename: R J
authors.initials: RJ
authors.lastname: Deshaies
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D002453:Cell Cycle; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D003001:Cloning, Molecular / Q000379:methods; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins / Q000235:genetics; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008025:Ligases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D013104:Spheroplasts / Q000502:physiology; D013698:Templates, Genetic; D014158:Transcription, Genetic; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases; D014452:Ubiquitins / Q000378:metabolism*
chemical_list: D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D011994:Recombinant Proteins; C086926:SIC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014452:Ubiquitins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D008025:Ligases