Isolation of 1-methyl-1,2,3,4-tetrahydroisoquinoline-synthesizing enzyme from rat brain: a possible Parkinson's disease-preventing enzyme.
An endogenous parkinsonism-preventing substance, 1-methyl-1,2,3,4-tetrahydroisoquinoline, is enzymatically formed from 2-phenethylamine and pyruvate in rat brain. The enzyme involved was localized in the mitochondrial-synaptosomal fraction of rat brain, solubilized by treatment with sodium deoxycholate. In order to purify the enzyme, gel filtration was carried out. This enzyme may be important in the pathogenesis of Parkinson's disease.
Yamakawa
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Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Japan.
Ohta
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D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D002850:Chromatography, Gel; D003840:Deoxycholic Acid; D006863:Hydrogen-Ion Concentration; D007546:Isoquinolines / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D010300:Parkinson Disease / Q000201:enzymology* / Q000517:prevention & control; D010627:Phenethylamines / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D012995:Solubility; D013574:Synaptosomes / Q000201:enzymology; D044005:Tetrahydroisoquinolines
D007546:Isoquinolines; D010627:Phenethylamines; D044005:Tetrahydroisoquinolines; D003840:Deoxycholic Acid; C029261:phenethylamine; C050386:1-methyl-1,2,3,4-tetrahydroisoquinoline; D019289:Pyruvic Acid