title: Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice.
abstract: The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.
authors.affiliation: Lund University Medical Laser Centre, Lund Institute of Technology, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Nilsson
authors.forename: J
authors.initials: J
authors.lastname: Johansson
authors.forename: K
authors.initials: K
authors.lastname: Svanberg
authors.forename: S
authors.initials: S
authors.lastname: Svanberg
authors.forename: G
authors.initials: G
authors.lastname: Jori
authors.forename: E
authors.initials: E
authors.lastname: Reddi
authors.forename: A
authors.initials: A
authors.lastname: Segalla
authors.forename: D
authors.initials: D
authors.lastname: Gust
authors.forename: A L
authors.initials: AL
authors.lastname: Moore
authors.forename: T A
authors.initials: TA
authors.lastname: Moore
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002338:Carotenoids / Q000378:metabolism*; D005354:Fibrosarcoma / Q000737:chemistry / Q000175:diagnosis; D008099:Liver / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011166:Porphyrins / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000737:chemistry / Q000175:diagnosis*; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution
chemical_list: D014408:Biomarkers, Tumor; D011166:Porphyrins; C090538:trimethoxycarotenoporphyrin; C090537:trimethylcarotenoporphyrin; D002338:Carotenoids