Distribution of transforming growth factor beta1-binding proteins and low-affinity receptors during odontoblast differentiation in the mouse.
Transforming growth factor-beta1 (TGF-beta1) was immunolocalized within differentiated odontoblasts and ameloblasts while LAP-beta1 was detected at the apicol pole of odonotoblasts and ameloblasts and in predentine. Anti-LAP-beta1 antibodies also stained the epithelial-mesenchymal junction (EMJ). Decorin was immunolocalized in young functional odonotoblasts and in both predentine and dentine. Biglycan was similarly distributed but absent from dentine. Immunostaining with anti-latent TGF-beta1 binding protein-1 (LTBP-1) showed fibrillar structures located at the EMJ and between predontoblasts and odontoblasts; at older states staining was restricted to the dental papilla and sac. Thus differentiated odonotoblasts express TGF-beta1 and in a more restricted manner decorin, biglycan and LAP-beta1; it can be assumed that TGF-beta1 is able to interact with the three molecules present in predentine. Earlier, LTBP-1 and LAP-beta1, both present at the EMJ, may contribute to odontoblast differentiation.
Cam
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Y
Institut de Biologie Médicale, INSERM U424, Faculté de Médecine 11, Strasbourg, France.
Lesot
H
H
Colosetti
P
P
Ruch
J V
JV
D030201:Activin Receptors, Type I; D000565:Ameloblasts / Q000166:cytology / Q000378:metabolism; D000818:Animals; D058578:Biglycan; D002352:Carrier Proteins / Q000032:analysis*; D002454:Cell Differentiation; D004396:Coloring Agents; D058575:Decorin; D003771:Dental Papilla / Q000166:cytology / Q000378:metabolism; D003795:Dental Sac / Q000166:cytology / Q000378:metabolism; D003804:Dentin / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D007150:Immunohistochemistry; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009804:Odontoblasts / Q000166:cytology* / Q000378:metabolism; D010446:Peptide Fragments; D011498:Protein Precursors; D017346:Protein Serine-Threonine Kinases / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis*; D016212:Transforming Growth Factor beta / Q000032:analysis*; D053773:Transforming Growth Factor beta1
C546885:Bgn protein, mouse; D058578:Biglycan; D002352:Carrier Proteins; D004396:Coloring Agents; C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; D010446:Peptide Fragments; D011498:Protein Precursors; D011506:Proteins; D011509:Proteoglycans; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D017346:Protein Serine-Threonine Kinases; D030201:Activin Receptors, Type I; D000077293:Receptor, Transforming Growth Factor-beta Type I