title: Methods for studying the biochemical properties of an Inr element binding protein: TFII-I.
abstract: Transcription initiation in eukaryotic mRNA coding genes is brought about by a host of general transcription factors, which assemble into a functional preinitiation complex (PIC) at the core promoter region, and gene-specific factors, which exert their effects on the rate and/or stability of the PIC. The core promoter region consists of a well-characterized TATA box and/or a less well-characterized pyrimidine-rich initiator element (Inr). While the biochemical mechanisms of TATA-mediated transcription initiation are extensively studied and known to be directed by the TATA binding protein, the mechanisms via the Inr element are poorly understood, as several factors have been shown to bind to an Inr. Here, we describe the biochemical properties of an Inr binding protein, TFII-I, employing the naturally occurring TATA-less but Inr-containing promoter derived from the T-cell receptor beta chain gene (V beta).
authors.affiliation: Program in Immunology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
authors.forename: C D
authors.initials: CD
authors.lastname: Novina
authors.forename: V
authors.initials: V
authors.lastname: Cheriyath
authors.forename: M C
authors.initials: MC
authors.lastname: Denis
authors.forename: A L
authors.initials: AL
authors.lastname: Roy
mesh_terms: D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D019169:Jurkat Cells; D009687:Nuclear Proteins / Q000276:immunology / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors