title: The effects of a triterpene fraction isolated from Crataegus monogyna Jacq. on different acute inflammation models in rats and mice. Leucocyte migration and phospholipase A2 inhibition.
abstract: The plant Crataegus monogyna has action against cardiac insufficiency, angina and arrhythmia. The anti-inflammatory properties of the cycloartenol fraction from this plant have been investigated. Chromatographic fractionation of the hexane extract of Crataegus monogyna Jacq. (Rosaceae) furnished a triterpene fraction containing cycloartenol as the main component (80.87%). The anti-inflammatory activity of the fraction was tested against hind-paw oedema induced by carrageenan in rats. At the highest oral dose (40 mg kg-1) inhibition was 61.5 and 52.5% at 3 and 5 h respectively. In the mouse carrageenan peritonitis test, the triterpene fraction given orally inhibited peritoneal leucocyte infiltration (41.9, 64.7 and 89.4% at 10, 20 and 40 mg kg-1, respectively). The fraction also showed weak inhibition of phospholipase A2 (PLA2) in-vitro. These results suggest that the fraction containing cycloartenol as the main component exerts an important anti-inflammatory action in-vivo by reducing the oedema.
authors.affiliation: Departamento de Farmacia, Tecnología Farmacéutica y Farmacología, Facultad de Farmacia, Sevilla, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Ahumada
authors.forename: T
authors.initials: T
authors.lastname: Sáenz
authors.forename: D
authors.initials: D
authors.lastname: García
authors.forename: R
authors.initials: R
authors.lastname: De La Puerta
authors.forename: A
authors.initials: A
authors.lastname: Fernandez
authors.forename: E
authors.initials: E
authors.lastname: Martinez
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002351:Carrageenan / Q000633:toxicity; D002464:Cell Migration Inhibition; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D007213:Indomethacin / Q000494:pharmacology; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D010538:Peritonitis / Q000097:blood / Q000139:chemically induced; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D010840:Phytosterols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D011239:Prednisolone / Q000494:pharmacology; D051381:Rats; D013030:Spain; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010840:Phytosterols; D010936:Plant Extracts; D014315:Triterpenes; D002351:Carrageenan; D011239:Prednisolone; D010741:Phospholipases A; D054467:Phospholipases A2; D007213:Indomethacin; C100089:cycloartenol