title: Intraepithelial lymphocytes in normal human intestine do not express proteins associated with cytolytic function.
abstract: Human small intestine contains a very large population of intraepithelial T lymphocytes (IELs) that are oligoclonal, appear functionally to be cytolytic T cells, and may contribute to the normal and pathological turnover of intestinal epithelial cells. This report addresses the cytolytic function of IELs in normal small intestine by examining their expression of molecules that carry out cell-mediated cytolysis. Immunohistochemical analyses of granzyme B, perforin, Fas ligand, and tumor necrosis factor-alpha demonstrated these proteins were not expressed by small intestinal IELs in situ. These proteins also were not expressed by colonic IELs or by lamina propria lymphocytes in the small or large intestine. Granzyme A, however, was expressed by a large fraction of IELs. In contrast to these in situ results, isolated and in vitro activated IELs were shown to express effector proteins consistent with cytolytic T cells, including granzyme B, Fas ligand, tumor necrosis factor-alpha, and interferon-gamma. These results are most consistent with the vast majority of IELs in normal human small intestine being resting cytolytic T cells and suggest that these cells do not contribute to the apoptotic cell death of epithelial cells in normal intestine.
authors.affiliation: Cancer Biology Program, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: A
authors.initials: A
authors.lastname: Chott
authors.forename: D
authors.initials: D
authors.lastname: Gerdes
authors.forename: A
authors.initials: A
authors.lastname: Spooner
authors.forename: I
authors.initials: I
authors.lastname: Mosberger
authors.forename: J A
authors.initials: JA
authors.lastname: Kummer
authors.forename: E C
authors.initials: EC
authors.lastname: Ebert
authors.forename: R S
authors.initials: RS
authors.lastname: Blumberg
authors.forename: S P
authors.initials: SP
authors.lastname: Balk
mesh_terms: D053222:Fas Ligand Protein; D053804:Granzymes; D006801:Humans; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis*; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012697:Serine Endopeptidases / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D014409:Tumor Necrosis Factor-alpha; D054353:Perforin; C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases