Crystal violet staining of Bordetella bronchiseptica colonies for differentiation of phase-I strains from variant strains in degraded phases.
After 2 days of growth on Brain heart infusion agar (BHIA) at 38 degrees C, phase-I colonies and degraded-phase colonies of Bordetella bronchiseptica could be differentiated by their ability to take up crystal violet (CV). Phase-I colonies in X mode, but not colonies in degraded phases (phases II, III, and rough) bound CV. Phenotypically-altered C-mode colonies (grown at 32 degrees C or lower temperatures) also lacked this ability. CV staining offers an easy method for the recognition of different colony types that appear identical when observed on BHIA.
Ishikawa
H
H
Hokkaido Research Station, National Institute of Animal Health, Hitsujiganka, Toyohira-ku, Sapporo, Japan.
Isayama
Y
Y
D000818:Animals; D016950:Bordetella bronchiseptica / Q000145:classification / Q000254:growth & development*; D015169:Colony Count, Microbial / Q000379:methods / Q000662:veterinary; D005840:Gentian Violet; D010641:Phenotype; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods / Q000662:veterinary
D012394:Rosaniline Dyes; D005840:Gentian Violet