Soluble selectins and ICAM-1 modulate neutrophil-endothelial adhesion and diapedesis in vitro.
We observed that normal plasma dramatically reduces neutrophil-endothelial adhesion. Therefore, we identified factors in plasma which might limit PMN adhesion in vitro. We found that the anti-adhesive effect was not mediated by vasoactive lipids present in plasma. Immunoprecipitation of soluble adhesion molecules, P and E-selectins and ICAM-1 restored PMN adhesion to control values. We further examined whether soluble adhesion molecules in plasma might also regulate PMN endothelial migration in response to fMLP (10(-6) M). Plasma significantly reduced PMN migration, and this effect was prevented only by the simultaneous removal of soluble P and E selectins and ICAM-1 together, but not individually. These data show that soluble selectins and ICAM-1 may regulate PMN adhesion and diapedesis, and that alterations in the levels of these molecules may regulate PMN-endothelial interactions in vivo.
Ohno
N
N
Department of Physiology, LSU Medical Center, Shreveport 71130, USA.
Ichikawa
H
H
Coe
L
L
Kvietys
P R
PR
Granger
D N
DN
Alexander
J S
JS
D000328:Adult; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology; D019040:E-Selectin / Q000097:blood / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000293:injuries / Q000502:physiology*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood / Q000502:physiology*; D008055:Lipids / Q000097:blood / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D019007:P-Selectin / Q000097:blood / Q000502:physiology; D019087:Selectins / Q000097:blood / Q000502:physiology*; D012995:Solubility
D019040:E-Selectin; D018836:Inflammation Mediators; D008055:Lipids; D019007:P-Selectin; D019087:Selectins; D018799:Intercellular Adhesion Molecule-1; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006493:Heparin