title: The Cryphonectria parasitica plasmid pUG1 contains a large ORF with motifs characteristic of family B DNA polymerases.
abstract: The isolation and characterization of the circular mitochondrial plasmid pUG1 from the ascomycete Cryphonectria parasitica is described. The entire sequence (4182 bp) was obtained and high similarities to DNA-dependent DNA polymerases were revealed. Strikingly common features with the DNA polymerases encoded by the Neurospora intermedia plasmids Fiji and LaBelle, such as matches to the conserved motifs A and B and the presence of TTD instead of DTD in motif C, were found, suggesting the existence of a distinct group of members of the B DNA family polymerases. These strong similarities between the plasmids might suggest a common origin of the C.parasitica and the Neurospora plasmids.
authors.affiliation: Dipartimento di Biologia applicata alla Difesa delle Piante, Università di Udine, Via Scienze 208, Udine I-33100, Italy. micol@pldef.uniud.it
authors.forename: E
authors.initials: E
authors.lastname: Gobbi
authors.forename: A
authors.initials: A
authors.lastname: Carpanelli
authors.forename: G
authors.initials: G
authors.lastname: Firrao
authors.forename: R
authors.initials: R
authors.lastname: Locci
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D010957:Plasmids; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D004259:DNA-Directed DNA Polymerase