Detection of hepatitis B surface gene mutation in carrier children with or without immunoprophylaxis at birth.
To investigate the clinical significance of mutation in hepatitis B virus (HBV) surface gene, DNA sequence analysis of the "a" determinant was performed on sera from 27 carrier children with immunoprophylaxis, their mothers, and 21 carriers without vaccination. A precore mutant (G to A) at nucleotide 1896 was detected in sera from 11 carriers without vaccination. Mutations in the "a" determinant were detected in 6 (22%) of the vaccinated children. Four HBV strains showed a Gly-to-Arg mutation at the 145th codon of the surface gene. Amino acid substitutions at amino acid 133 and 144 were noted in the other 2 children. Only 1 mother had the same predominant strain of mutant virus as her child. These observations indicate that immune pressure exerted by immunoprophylaxis at birth may select for a mutant virus.
Lee
P I
PI
Department of Pediatrics, National Taiwan University Hospital, Taipei.
Chang
L Y
LY
Lee
C Y
CY
Huang
L M
LM
Chang
M H
MH
D000293:Adolescent; D000328:Adult; D002353:Carrier State; D002648:Child; D004252:DNA Mutational Analysis; D005260:Female; D005814:Genes, Viral / Q000235:genetics; D006509:Hepatitis B / Q000517:prevention & control / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D017325:Hepatitis B Vaccines; D006513:Hepatitis B e Antigens; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D017354:Point Mutation / Q000235:genetics*; D013624:Taiwan; D017211:Treatment Failure; D014611:Vaccination; D015678:Viral Structural Proteins / Q000235:genetics
D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D006513:Hepatitis B e Antigens; D015678:Viral Structural Proteins