High-volume cellular screening for anticancer agents with combinatorial chemical libraries: a new methodology.
A single-step cancer cell cytotoxic assay system for anticancer drug discovery has been developed which facilitates rapid screening of large combinatorial chemical libraries synthesized using the 'one-bead-one-compound' (OBOC) methodology. Each OBOC library bead incorporates two orthogonally cleavable linkers that release the bead-bound compound at a different pH. The assay utilizes high concentrations of tumor cells mixed directly with OBOC beads and plated in soft agarose containing tissue culture medium. One of the orthogonal linkers is cleaved at neutral pH in tissue culture releasing an aliquot of compound to diffuse at a relatively high local concentration into the soft agarose immediately surrounding the bead. Active compounds are identified visually from a clear ring of tumor cell lysis which forms within 48 h around just the rare bead releasing a cytotoxic compound. The bead releasing a cytotoxin is then plucked from the agar and the remaining compound still linked to the bead can be released for structural analysis, followed by compound resynthesis and confirmatory testing. This assay system has been successfully applied to identification of lead cytotoxic compounds from model peptidic and non-peptidic combinatorial chemical libraries. Use of this methodology may facilitate anticancer drug discovery.
Salmon
S E
SE
Arizona Cancer Center, University of Arizona, Tucson 85724, USA.
Liu-Stevens
R H
RH
Zhao
Y
Y
Lebl
M
M
Krchñák
V
V
Wertman
K
K
Sepetov
N
N
Lam
K S
KS
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy; D046508:Culture Techniques; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D007941:Leukemia P388 / Q000188:drug therapy; D051379:Mice; D008863:Microspheres; D009101:Multiple Myeloma / Q000188:drug therapy; D019151:Peptide Library; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D019151:Peptide Library