Retention of functional characteristics by bovine oviduct and uterine epithelia in vitro.
The composition of fluids within the bovine oviduct and uterine lumen, important in fertilisation and early embryonic development, is ultimately determined by the transport properties of the epithelial cells which line the lumen. A preparation has therefore been devised to study the role of these cells in oviduct and uterine fluid formation. Pure preparations of epithelial cells, as judged immunocytochemically, were isolated by enzyme digestion, and grown on collagen filters in primary culture. The cells re-establish intercellular junctions to form a confluent epithelial layer. Serial samples from the apical and basal media were analysed for K+, Na+, Ca2+, glucose and lactate. Bovine oviduct epithelial cells maintained gradients of K+ and Ca2+ (apical > basal) for up to 14 days after confluence, while bovine uterine epithelial cells maintained apical > basal gradients of K+. Both types of epithelium exhibited a small transepithelial electrical potential difference and a higher uptake of glucose and production of lactate in the basal, as opposed to apical medium. There were no consistent differences in any of these parameters with the stage of the oestrous cycle at which the cells were removed. The data indicate that bovine oviduct and uterine epithelia may be isolated and grown as polarised layers in primary culture. The preparations will now enable the mechanisms underlying the secretion of ions and non-electrolytes to be determined.
Cox
C I
CI
Department of Biology, University of York, UK.
Leese
H J
HJ
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002417:Cattle / Q000378:metabolism / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D005187:Fallopian Tubes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D005260:Female; D005947:Glucose / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D007633:Keratins / Q000032:analysis; D007773:Lactates / Q000032:analysis / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D011188:Potassium / Q000032:analysis / Q000378:metabolism; D012964:Sodium / Q000032:analysis / Q000378:metabolism; D014599:Uterus / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
D007773:Lactates; D007633:Keratins; D012964:Sodium; D005947:Glucose; D011188:Potassium; D002118:Calcium