title: Rapid analysis of malathion in blood using head space-solid phase microextraction and selected ion monitoring.
abstract: A simple and rapid method for analysis of malathion in blood was developed using head space-solid phase microextraction (HS-SPME) and gas chromatography mass spectrometry/ electron impact ionization-selected ion monitoring (GC-MS/EI-SIM). A vial containing a blood sample, ammonium sulphate, sulphuric acid and fenitrothion as an internal standard, was heated at 90 degrees C for 15 min. The extraction fiber of the SPME was exposed for 5 min in the head space of the vial. The compounds absorbed on the fiber were detached by exposing the fibre in the injection port of GC-MS. A straight calibration curve was obtained between malathion concentrations of 2.5 to 50.0 micrograms g-1 in blood. No interfering substances were found, and the time for analysis was 40 min for one sample.
authors.affiliation: Department of Legal Medicine, Hiroshima University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Namera
authors.forename: M
authors.initials: M
authors.lastname: Yashiki
authors.forename: N
authors.initials: N
authors.lastname: Nagasawa
authors.forename: Y
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: T
authors.initials: T
authors.lastname: Kojima
mesh_terms: D000328:Adult; D001826:Body Fluids / Q000737:chemistry; D002138:Calibration; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007306:Insecticides / Q000097:blood*; D008294:Malathion / Q000097:blood* / Q000506:poisoning; D008297:Male; D008875:Middle Aged
chemical_list: D007306:Insecticides; D008294:Malathion