title: [Evaluation of reticulocyte count on Cell-Dyn 3500 automate: comparison with a flow cytometry technique].
abstract: Cell-Dyn 3500 automate (Abbott) provides reticulocyte count using a recent software, after ribosomal RNA colouring with methylene blue reagent. The aim of this study was to evaluate results from Cell-Dyn 3500, and to compare them with the routine laboratory technique by flow cytometry (FacScan, Becton-Dickinson). Cell-Dyn 3500 analytical parameters (inter- and intra-assay precision, sample-to-sample contamination) were good and in accord to manufacturer's specifications. Reticulocyte values from blood samples kept at 4 degrees C during 3 days were unchanged. Usual values in 145 normal patients were found between 71.3 and 78.3 G/l with Cell-Dyn 3500, between 119.3 and 130.2 G/l with FacScan. Both techniques were compared in 87 patients with various anemias: Cell-Dyn 3500 showed more frequently high reticulocyte values in regenerative anemias (hemorrhagic), but was less performant for classification of aregenerative anemias. Reticulocyte count with Cell-Dyn 3500 is a semi automatic, easy to perform and swift technique, using no toxic reagent but unitary test tubes kept at room temperature. The test cost is however high, and Cell-Dyn 3500 cannot be used during the analysis.
authors.affiliation: Laboratoire de biologie, Centre hospitalier Gilles-de-Corbeil, Corbeil-Essonnes.
authors.forename: C
authors.initials: C
authors.lastname: Alvarez
authors.forename: C
authors.initials: C
authors.lastname: Chabert
authors.forename: P
authors.initials: P
authors.lastname: Quillet
authors.forename: H
authors.initials: H
authors.lastname: Baufine-Ducrocq
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D017701:Reticulocyte Count / Q000295:instrumentation / Q000379:methods*; D012984:Software
vernacular_title: Evaluation de la numération des réticulocytes sur automate Cell-Dyn 3500: comparaison avec une technique par cytométrie en flux.