Estrogen does not induce the calcium-dependent nitric oxide synthase in cultured human uterine endothelial and myometrial smooth muscle cells.
In many tissues, estrogen-induced vasodilatation is mediated, at least in part, by the release of nitric oxide (NO). We determined whether human myometrial endothelial and smooth muscle cells express estrogen receptors (ERs) and whether endothelial NO synthase (eNOS) expression in these cells was affected by 17beta-estradiol (10[-13]-10[-6]M). ER was strongly expressed in myometrial smooth muscle cells but was absent from endothelial cells. Expression of eNOS mRNA was strong in endothelial cells, but weak in muscle cells. 17beta-estradiol administration for 24 or 72 h failed to increase eNOS in both cell types. Thus, an increase of human uterine blood flow by estrogens appears not to be mediated by stimulation of myometrial eNOS expression.
Tschugguel
W
W
Department of Obstetrics and Gynecology, University of Vienna School of Medicine, Austria. walter.tschugguel@akh-wien.ac.at
Zhegu
Z
Z
Schneeberger
C
C
Tantscher
E
E
Czerwenka
K
K
Fabry
A
A
Wojta
J
J
Zeillinger
R
R
Huber
J C
JC
D000328:Adult; D000368:Aged; D002118:Calcium / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004717:Endometrium / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D009215:Myometrium / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D014599:Uterus / Q000201:enzymology*; D014841:von Willebrand Factor / Q000378:metabolism
D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014841:von Willebrand Factor; D004958:Estradiol; D019001:Nitric Oxide Synthase; D002118:Calcium