Structural organization and chromosomal assignment of the swine endothelin-1 gene.
Cosmid clone containing swine endothelin-1 (EDN1) gene, cosEDN1, was isolated from swine cosmid library using swine EDN1 cDNA as a probe. The sequence analysis of cosEDN1 DNA revealed that the swine EDN1 gene consists of 5 exons, spanning approximately 6.5 kb. In the 5'-upstream region of the EDN1 gene, AP-1 and NF-1 elements were found, suggesting the possibility that the expression of swine EDN1 gene is controlled by protooncogene products Fos and Jun, and TGF-beta. Fluorescence in situ hybridization (FISH) using cosEDN1 DNA as a probe demonstrated that EDN1 gene resides on swine chromosome 7p13- > pter.
Kojima
M
M
Animal Genome Research Group, National Institute of Animal Industry, Norindanchi, Japan.
Ohata
K
K
Yasue
H
H
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002999:Clone Cells; D003360:Cosmids / Q000235:genetics*; D019332:Endothelin-1 / Q000235:genetics*; D015870:Gene Expression; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016760:Proto-Oncogene Proteins c-fos / Q000502:physiology; D016755:Proto-Oncogene Proteins c-jun / Q000502:physiology; D013552:Swine / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000502:physiology
D019332:Endothelin-1; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D016212:Transforming Growth Factor beta