title: Serrate-mediated activation of Notch is specifically blocked by the product of the gene fringe in the dorsal compartment of the Drosophila wing imaginal disc.
abstract: In the developing imaginal wing disc of Drosophila, cells at the dorsoventral boundary require localized Notch activity for specification of the wing margin. The Notch ligands Serrate and Delta are required on opposite sides of the presumptive wing margin and, even though activated forms of Notch generate responses on both sides of the dorsoventral boundary, each ligand generates a compartment-specific response. In this report we demonstrate that Serrate, which is expressed in the dorsal compartment, does not signal in the dorsal regions due to the action of the fringe gene product. Using ectopic expression, we show that regulation of Serrate by fringe occurs at the level of protein and not Serrate transcription. Furthermore, replacement of the N-terminal region of Serrate with the corresponding region of Delta abolishes the ability of fringe to regulate Serrate without altering Serrate-specific signaling.
authors.affiliation: Department of Biology, The University of Rochester, NY 14627, USA. flem@uhura.cc.rochester.edu
authors.forename: R J
authors.initials: RJ
authors.lastname: Fleming
authors.forename: Y
authors.initials: Y
authors.lastname: Gu
authors.forename: N A
authors.initials: NA
authors.lastname: Hukriede
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics* / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000235:genetics*; D017351:N-Acetylglucosaminyltransferases; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction / Q000235:genetics; D014158:Transcription, Genetic / Q000502:physiology; D014921:Wings, Animal / Q000196:embryology
chemical_list: D002135:Calcium-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; C000606278:Ser protein, Drosophila; D000072099:Serrate-Jagged Proteins; C064045:delta protein; D017351:N-Acetylglucosaminyltransferases; C090576:fng protein, Drosophila