Analytical derivatizations of volatile and hydrophilic carbonyls from aqueous matrix onto a solid phase of a polystyrene-divinylbenzene macroreticular resin.
Extraction and derivatization of carbonyls to benzyloximes, pentafluorobenzyloximes or 2,4-dinitrophenylhydrazones is simplified and reaction times are substantially reduced by simultaneous sorption and derivatization from aqueous solution onto a solid phase. In this reaction a macroreticular polystyrene-divinylbenzene resin acts as a sorbent and catalyst to allow simultaneous extraction and derivatization of hydrophilic and lipophilic aldehydes and ketones from simple as well as complex matrices including plasma. Conversion to the 2,4-dinitrophenylhydrazones or pentafluorobenzyloximation at ambient temperature requires 10 and 20 min, respectively. These reaction conditions correspond to at least a 6-fold reduction in reaction times for derivatization of the reactive aldelhydes and a 36-72-fold reduction for preparation of derivatives for the slower reacting ketones.
Breckenridge
S M
SM
Department of Pathology, McMaster University, Hamilton, Ont., Canada.
Yin
X
X
Rosenfeld
J M
JM
Yu
Y H
YH
D000447:Aldehydes / Q000097:blood / Q000737:chemistry*; D017130:Angioplasty; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006898:Hydroxylamines / Q000737:chemistry; D007475:Ion Exchange Resins; D007659:Ketones / Q000097:blood / Q000737:chemistry*; D017240:Mitochondrial Myopathies / Q000097:blood / Q000188:drug therapy; D010084:Oxidation-Reduction; D010091:Oximes / Q000737:chemistry; D010659:Phenylhydrazines / Q000737:chemistry; D011137:Polystyrenes; D014815:Vitamins / Q000627:therapeutic use
D000447:Aldehydes; D006898:Hydroxylamines; D007475:Ion Exchange Resins; D007659:Ketones; D010091:Oximes; D010659:Phenylhydrazines; D011137:Polystyrenes; D014815:Vitamins; C004787:2,4-dinitrophenylhydrazine; C017064:benzyloxyamine; C003771:Amberlite XAD-2 resin