title: Human monoclonal antibodies to human blood group antigens Kidd Jka and Jkb.
abstract: Three IgM human monoclonal antibodies to Jkb, and one IgM human monoclonal antibody to Jka were produced from the lymphocytes of two immunized donors. Two of the anti-Jkb monoclonal antibodies (MS-7 and MS-9) are of the IgM (kappa) isotype and one (MS-8) is an IgM(lambda). The anti-Jka monoclonal antibody (MS-15) is of the IgM(kappa) isotype. They are all specific for their respective antigens, and give positive agglutinations in saline by the immediate spin technique, even against Jk(a+b+) cells. The heterohybridomas have been shown to be suitable for bulk culture and produce levels of antibody that reach 18 micrograms/ml in the spent culture supernatant. They offer considerable advantages over currently available reagents in terms of stability, simplicity and speed of use, and will provide a reliable and unlimited supply of what are at the moment rare and unsatisfactory antibodies.
authors.affiliation: Department of Biochemistry, Polytechnic of East London, U.K.
authors.forename: K
authors.initials: K
authors.lastname: Thompson
authors.forename: G
authors.initials: G
authors.lastname: Barden
authors.forename: J
authors.initials: J
authors.lastname: Sutherland
authors.forename: I
authors.initials: I
authors.lastname: Beldon
authors.forename: M
authors.initials: M
authors.lastname: Melamed
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D006801:Humans; D006825:Hybridomas; D007075:Immunoglobulin M / Q000276:immunology; D007667:Kidd Blood-Group System / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D007667:Kidd Blood-Group System