title: The presence of a transcription activation function in the hormone-binding domain of androgen receptor is revealed by studies in yeast cells.
abstract: To assess the importance of various regions of the androgen receptor (AR) in transcriptional regulation, we have compared its activation functions (AFs) in yeast and mammalian cells. The receptor's amino-terminal region contains a major transcriptional activator (AF-1) in both cell types, whereas AF-2 in the ligand-binding domain (LBD) is very weak in mammalian cells but clearly functional in the yeast. Hormone-binding ability of LBD is mandatory for AF-2 to operate, as illustrated by mutated LBD constructs. The activity of AF-2 in yeast is severely attenuated when the hinge region is attached to LBD, suggesting that the former region modulates AF-2 in vivo, probably by presenting an interface for interacting proteins.
authors.affiliation: Department of Physiology, Institute of Biomedicine, University of Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Moilanen
authors.forename: N
authors.initials: N
authors.lastname: Rouleau
authors.forename: T
authors.initials: T
authors.lastname: Ikonen
authors.forename: J J
authors.initials: JJ
authors.lastname: Palvimo
authors.forename: O A
authors.initials: OA
authors.lastname: Jänne
mesh_terms: D000728:Androgens / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D015534:Trans-Activators / Q000494:pharmacology*
chemical_list: D000728:Androgens; D011944:Receptors, Androgen; D015534:Trans-Activators