title: Gene identification using the yeast two-hybrid system.
authors.affiliation: Verna and Marrs McLean Department of Biochemistry, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: C
authors.initials: C
authors.lastname: Bai
authors.forename: S J
authors.initials: SJ
authors.lastname: Elledge
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular / Q000379:methods*; D004268:DNA-Binding Proteins; D004926:Escherichia coli; D005656:Fungal Proteins / Q000096:biosynthesis; D015723:Gene Library; D017930:Genes, Reporter; D006836:Hydro-Lyases / Q000096:biosynthesis*; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D055503:Protein Multimerization; D016364:Reading Frames; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015203:Reproducibility of Results; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013728:Terminator Regions, Genetic; D014157:Transcription Factors; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000096:biosynthesis*
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D007202:Indicators and Reagents; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D001616:beta-Galactosidase; D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase