title: Teratogenicity of Aino virus in the chick embryo.
abstract: Aino virus (JaNAr 28 strain), a possible agent of a congenital anomaly of calves, was inoculated into the yolk sac of chick embryos at a dose of 10(2), 10(3), 10(4) or 10(5) TCID50 0.2 ml-1 at four, six or eight days of incubation. At 21 days of incubation all the unhatched embryos or hatched chickens in the experimental and control groups were sacrificed for pathological examination. The incidence of hydranencephaly, cerebellar hypoplasia or agenesis and arthrogryposis or scoliosis was highest (85 per cent in the chicks inoculated with 10(3) TCID50 0.2 ml-1 at eight days of incubation. The lesions were very similar to those found in congenital abnormalities in calves suspected of a natural infection with Aino virus.
authors.affiliation: Laboratory of Veterinary Pathology, Faculty of Agriculture, Kagoshima University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kitano
authors.forename: N
authors.initials: N
authors.lastname: Yasuda
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Ohzono
authors.forename: T
authors.initials: T
authors.lastname: Iwamoto
mesh_terms: D000818:Animals; D001176:Arthrogryposis / Q000453:epidemiology / Q000209:etiology / Q000662:veterinary; D002044:Bunyaviridae Infections / Q000150:complications / Q000453:epidemiology / Q000662:veterinary*; D002526:Cerebellar Diseases / Q000453:epidemiology / Q000209:etiology / Q000662:veterinary; D002531:Cerebellum / Q000002:abnormalities; D002642:Chick Embryo / Q000002:abnormalities / Q000254:growth & development / Q000821:virology*; D002645:Chickens; D000013:Congenital Abnormalities / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004487:Edema / Q000453:epidemiology / Q000209:etiology / Q000662:veterinary; D006832:Hydranencephaly / Q000453:epidemiology / Q000209:etiology / Q000662:veterinary; D015994:Incidence; D018482:Muscle, Skeletal / Q000002:abnormalities; D011201:Poultry Diseases / Q000453:epidemiology / Q000821:virology*; D012600:Scoliosis / Q000453:epidemiology / Q000209:etiology / Q000662:veterinary; D012840:Simbu virus / Q000502:physiology*; D013997:Time Factors