Chromosome aberrations in human spermatozoa treated with Ca2+ ionophore A23187.
Incorporation of A23187 ionophore into the human-hamster fertilization system clearly improves the ability of human spermatozoa to penetrate zona-free hamster oocytes. Thus, an increasing number of laboratories working in human sperm cytogenetics have substituted classical incubation with Biggers-Whitten-Whittingham (BWW) medium plus human serum albumin (HSA) by pretreatment of spermatozoa with calcium ionophore A23187 which directly induces the acrosome reaction in spermatozoa. However, there have been no formal studies on the effects of this ionophore pretreatment. To determine whether calcium ionophore could affect the cytogenetic characteristics of human spermatozoa we compared A23187-treated spermatozoa with controls (only incubated with BWW + HSA) by analysing a total of 447 sperm chromosome complements from two normal donors. Our results show that there are no statistical differences in the frequency and the types of human sperm chromosomal abnormalities between the two methods of sperm treatment. Thus, ionophore A23187 seems not to affect the cytogenetic characteristics of human spermatozoa, and the results of laboratories using either sperm capacitation in BWW + HSA or acrosome reaction by calcium ionophore can be compared.
Alvarez
R
R
Departament de Biologia Cel-lular i Fisiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Tusell
L
L
Genescà
A
A
Miró
R
R
Benet
J
J
Caballín
M R
MR
Egozcue
J
J
D000177:Acrosome / Q000187:drug effects; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D006224:Cricetinae; D005260:Female; D006801:Humans; D007476:Ionophores / Q000494:pharmacology*; D008297:Male; D013075:Sperm Capacitation / Q000187:drug effects; D013084:Sperm-Ovum Interactions / Q000187:drug effects / Q000235:genetics; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects*
D007476:Ionophores; D000001:Calcimycin; D002118:Calcium