title: Mechanisms of action of the bactericidal/permeability-increasing protein BPI on endotoxin and phospholipid monolayers and aggregates.
abstract: We have investigated the mechanisms of interaction of the recombinant N-terminal portion of bactericidal/permeability-increasing protein, rBPI21, with lipopolysaccharide (LPS) isolated from enterobacterial deep rough mutant strains. Experimentally, the ability of rBPI21 to form monolayers at the air/water interface and its action on lipid monolayers were analyzed. We have further studied the interaction of rBPI21 with aggregates from phospholipids and Re mutant LPS by infrared and resonance energy transfer spectroscopy and laser Doppler velocimetry. From monolayer experiments, the molecular area of a single rBPI21 molecule was estimated to be about 12 nm2. At lateral pressures of </=25 mN/m, rBPI21 incorporated into monolayers from negatively charged LPS and phosphatidylglycerol (PG) but not into those from neutral phosphatidylcholine. rBPI21 incorporated not only into monolayers but also into liposomes made from or containing negatively charged phospholipids, reducing the absolute value of the zeta-potential of LPS and PG aggregates. Furthermore, due to intercalation, rBPI21 caused the rigidification of the acyl chains of lipids in the gel as well as in the fluid phase and significantly immobilized their phosphate groups. High concentrations of Mg2+ ions were found to have a protective effect against the action of rBPI21. On the basis of these results, the biophysical characteristics of rBPI21 are discussed and a model is proposed as to how the rBPI21-induced influence on lipid monolayers and bilayers could explain rBPI21-mediated effects on the bacterial membrane.
authors.affiliation: Department of Immunochemistry and Biochemical Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Parkallee 10, D-23845 Borstel, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Wiese
authors.forename: K
authors.initials: K
authors.lastname: Brandenburg
authors.forename: B
authors.initials: B
authors.lastname: Lindner
authors.forename: A B
authors.initials: AB
authors.lastname: Schromm
authors.forename: S F
authors.initials: SF
authors.lastname: Carroll
authors.forename: E T
authors.initials: ET
authors.lastname: Rietschel
authors.forename: U
authors.initials: U
authors.lastname: Seydel
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry*; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000737:chemistry*; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010715:Phosphatidylglycerols / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D010715:Phosphatidylglycerols; D010743:Phospholipids; C021623:bactericidal permeability increasing protein; C002773:dimyristoylphosphatidylglycerol