Calibration of micropipets using the bioluminescent protein aequorin.
A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.
Grosvenor
A L
AL
Department of Chemical and Materials Engineering, University of Kentucky, Lexington 40506-0055, USA.
Crofcheck
C L
CL
Anderson
K W
KW
Scott
D L
DL
Daunert
S
S
D000331:Aequorin; D002138:Calibration; D004864:Equipment and Supplies / Q000592:standards*; D008163:Luminescent Measurements; D008853:Microscopy; D015203:Reproducibility of Results
D000331:Aequorin