Association of interleukin-6 in the cerebrospinal fluid during crisis of calf with ammoniated feed syndrome.
Ammoniated feed syndrome (AFS) in cattle is a neurotoxic syndrome caused by feeding specific ammoniated forage. To clarify the pathophysiology of AFS, we examined the association of interleukin-6 (IL-6) in the brain. By feeding milk either from cows fed such ammoniated forage or milk added with 4-methyl-imidazole, newborn calves showed a neurotoxic crisis of hyperexcitability, ataxia, muscle tremor, circling, roaring, epileptoid seizure, sweating and marked fever response. Although these calves had no pathological lesions in the brain, we detected a rise in IL-6 in the cerebrospinal fluid (CSF). Tumor necrosis factor-alpha (TNF-alpha) was not detected in the CSF. In the sera, IL-6 and TNF-alpha hardly changed during the experiment. Administration of recombinant human IL-6 into the lateral ventricle resulted in fever. Thus, we believe IL-6 in the CSF is related to the fever response in newborn calves with AFS.
Nakajima
Y
Y
National Institute of Animal Health, Tsukuba, Japan.
Yoshioka
M
M
Mikami
O
O
Arai
S
S
Miyamoto
S
S
Manda
T
T
Yamanaka
N
N
Hirose
H
H
Motoi
Y
Y
D000208:Acute Disease; D000641:Ammonia / Q000009:adverse effects*; D000821:Animal Feed / Q000009:adverse effects*; D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000134:cerebrospinal fluid* / Q000139:chemically induced*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood / Q000134:cerebrospinal fluid*; D008297:Male; D008892:Milk / Q000009:adverse effects; D011749:Pyrogens / Q000009:adverse effects; D013577:Syndrome; D014409:Tumor Necrosis Factor-alpha / Q000134:cerebrospinal fluid / Q000378:metabolism
D015850:Interleukin-6; D011749:Pyrogens; D014409:Tumor Necrosis Factor-alpha; D000641:Ammonia