The adaptive immune response to major surgery in the neonate.
The effect of major surgery on components of the adaptive immune response in babies has not previously been reported. In a prospective study, eight neonates undergoing uncomplicated surgery for repair of esophageal atresia were investigated. They were compared with ten age-matched normal babies not undergoing surgery. The parameters of the immune response investigated were: total leukocytes (WBC), lymphocytes and their subsets (T-helper, T-suppressor, natural killer [NK], B-lymphocytes), monocytes, immunoglobulins (Ig) G and M, the cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin 1-beta (Il-1beta), and C-reactive protein (CRP), an acute-phase protein. When compared to the normal controls, the operated group showed a fall in all types of WBC following surgery, but only the falls in B-lymphocytes and NK cells were significant on postoperative day 3 (P < 0.05). The suppression in WBC was temporary, and by day 7 the operated group had significantly higher numbers of total WBC and T-helper cells than the controls (P < 0.05), who were undergoing their physiological postpartum fall in WBC. Within the operated group, there was a significant fall in the numbers of total lymphocytes, T-suppressors, and B-lymphocytes compared to preoperative levels (P < 0.01). The ratio of T-helper/T-suppressor cells increased significantly following surgery. There was a vigorous immune response in terms of the humoral factors: CRP, TNF-alpha, and Il-1beta all rose significantly postoperatively (P < 0.02).
Grant
H W
HW
Department of Paediatric Surgery, University of Natal, P.O. Box 17039, Congella 4013, South Africa.
Chuturgoon
A A
AA
Kenoyer
D G
DG
Doorasamy
T
T
D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D004933:Esophageal Atresia / Q000276:immunology* / Q000601:surgery*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007231:Infant, Newborn / Q000276:immunology*; D007375:Interleukin-1 / Q000097:blood; D007958:Leukocyte Count; D018655:Lymphocyte Count; D008297:Male; D011446:Prospective Studies; D014138:Tracheoesophageal Fistula / Q000276:immunology / Q000601:surgery; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein