Expressional downregulation of neuronal-type NO synthase I in guinea pig skeletal muscle in response to bacterial lipopolysaccharide.
We have investigated the expression of neuronal-type NO synthase I (NOS I) and inducible-type NOS II in guinea pig skeletal muscle (diaphragm). Expression of NOS I mRNA and protein was highest in muscle of specific pathogen-free animals, lower in normally bred animals, and lowest in lipopolysaccharide (LPS)-treated animals. NOS II mRNA and protein levels were highest in muscle of LPS-treated animals. Elevated NOS activity in muscle from LPS-treated animals was less susceptible to the NOS I-selective inhibitor N(G)-nitro-L-arginine. Expressional downregulation of NOS I in sepsis may have implications for contractile function of skeletal muscle.
Gath
I
I
Department of Pharmacology, Johannes Gutenberg University, Mainz, Germany. gath@mzdmza.zdv.uni-mainz.de
Gödtel-Armbrust
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U
Förstermann
U
U
D000818:Animals; D015536:Down-Regulation; D006168:Guinea Pigs; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism
D008070:Lipopolysaccharides; D019001:Nitric Oxide Synthase