Isoelectric properties of the soluble matrices in relation to the chemical composition of some Scleractinian skeletons.
Soluble matrices of four Scleractinian skeletons (Madrepora, Favia, Leptastrea and Fungia), were extracted and studied by isoelectric focusing (IEF). The main part of the extracted matrices is acidic. IEF gels are poorly stained or unstained by Coomassie Blue and silver. The positive Alcian Blue staining is indicative of a sulfated and acidic nature of the glycoproteins. Chemical analysis shows high S contents in Scleractinian skeletons. These results are supported by the comparison with IEF and chemical contents of Molluscan shells.
Dauphin
Y
Y
Laboratoire de Paléontologie, URA 723 du CNRS, Université Paris XI-Orsay, Orsay, France. dauphin@geophy.geol.u-psud.fr
Cuif
J P
JP
D000818:Animals; D003063:Cnidaria / Q000737:chemistry*; D007525:Isoelectric Focusing / Q000379:methods*; D012995:Solubility