Identification of cooperative folding units in a set of native proteins.
Cooperative unfolding penalties are calculated by statistically evaluating an ensemble of denatured states derived from native structures. The ensemble of denatured states is determined by dividing the native protein into short contiguous segments and defining all possible combinations of native, i.e., interacting, and non-native, i.e., non-interacting, segments. We use a novel knowledge-based scoring function, derived from a set of non-homologous proteins in the Protein Data Bank, to describe the interactions among residues. This procedure is used for the structural identification of cooperative folding cores for four globular proteins: bovine pancreatic trypsin inhibitor, horse heart cytochrome c, French bean plastocyanin, and staphylococcal nuclease. The theoretical folding units are shown to correspond to regions that exhibit enhanced stability against denaturation as determined from experimental hydrogen exchange protection factors. Using a sequence similarity score for related sequences, we show that, in addition to residues necessary for enzymatic function, those amino acids comprising structurally important folding cores are also preferentially conserved during evolution. This implies that the identified folding cores may be part of an array of fundamental structural folding units.
Wallqvist
A
A
Frederick Cancer Research and Development Center, National Cancer Institute, Maryland 21702, USA. wallqvis@ncifcrf.gov
Smythers
G W
GW
Covell
D G
DG
D000595:Amino Acid Sequence; D000818:Animals; D007611:Aprotinin / Q000737:chemistry*; D003574:Cytochrome c Group / Q000737:chemistry*; D007887:Fabaceae / Q000378:metabolism; D006736:Horses; D008836:Micrococcal Nuclease / Q000737:chemistry*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D010946:Plants, Medicinal; D010970:Plastocyanin / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding
D003574:Cytochrome c Group; D010970:Plastocyanin; D007611:Aprotinin; D008836:Micrococcal Nuclease