title: Elevated serum HLA class I levels coincide with acute and chronic graft-versus-host disease.
abstract: The ability to predict the likely occurrence of graft-versus-host-disease (GVHD) after BMT would be extremely valuable. We performed a retrospective study on the correlation between soluble HLA class I (sHLA-I) levels and GVHD in the sera of 34 patients receiving an allogeneic BMT and in the sera of 12 patients receiving an autologous BMT. sHLA-I levels measured pre- and at different times post-BMT were correlated with the occurrence of post-BMT complications, ie acute graft-versus-host disease (aGVHD), chronic graft-versus-host disease (cGVHD), infections and relapse. No changes in sHLA-I levels (delta sHLA-I) occurred in autologous and allogeneic BMT patients without GVHD. In contrast, sHLA-I reached high levels in patients suffering from GVHD. Increased sHLA-I levels correlated strongly with episodes of both acute and chronic GVHD (P = 0.004 and P = 0.005, respectively). Also during relapse increased sHLA-I levels were found (P = 0.032). During infections sHLA-I levels increased, although not significantly. Kinetic studies gave no evidence that the increase in sHLA-I levels preceded the clinical occurrence of aGVHD or of cGVHD. A slight, but significant correlation was found between total blood bilirubin levels and sHLA-I levels in patients suffering from GVHD (P = 0.037), indicating the contribution of the liver as a source of sHLA-I. We conclude that measurements of sHLA-I levels do not function as a predictive parameter for GVHD, but can be valuable for the monitoring of GVHD after BMT.
authors.affiliation: Department of Immunohematology and Blood Bank, Leiden University Hospital, The Netherlands.
authors.forename: L M
authors.initials: LM
authors.lastname: Liem
authors.forename: C A
authors.initials: CA
authors.lastname: Koelman
authors.forename: I I
authors.initials: II
authors.lastname: Doxiadis
authors.forename: J C
authors.initials: JC
authors.lastname: van Houwelingen
authors.forename: E
authors.initials: E
authors.lastname: Goulmy
authors.forename: F H
authors.initials: FH
authors.lastname: Claas
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D002908:Chronic Disease; D005260:Female; D006086:Graft vs Host Disease / Q000097:blood / Q000209:etiology / Q000276:immunology*; D019337:Hematologic Neoplasms / Q000628:therapy*; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D008297:Male; D014184:Transplantation, Homologous
chemical_list: D015395:Histocompatibility Antigens Class I