title: Screening for Salmonella with a murine monoclonal antibody M105 detects both Felix O1 bacteriophage sensitive and resistant Salmonella strains.
abstract: Ten Felix O1 (FO1) bacteriophage sensitive Salmonella strains as well as their phage resistant derivates together with 39 strains of FO1-resistant Salmonella were tested for their reactivities with a murine monoclonal antibody, M105, by indirect whole cell and competitive ELISA. All FO1 phage sensitive and 48 of the 49 FO1-resistant Salmonella strains were found to react with M105. The single Salmonella strain not reacting with M105 was a FO1 resistant derivative selected by exposing the sensitive parent strain to the phage. This M105-negative and FO1-resistant strain was also found to be a rough mutant without O-antigens and possibly lacks the terminal LPS core sugars which form the M105 reactive epitope.
authors.affiliation: National Laboratory for Enteric Pathogens, Bureau of Microbiology, Health Canada. rtsang@hpb.hwc.ca
authors.forename: R S
authors.initials: RS
authors.lastname: Tsang
authors.forename: K H
authors.initials: KH
authors.lastname: Nielsen
authors.forename: D M
authors.initials: DM
authors.lastname: Henning
authors.forename: C
authors.initials: C
authors.lastname: Poppe
authors.forename: R
authors.initials: R
authors.lastname: Khakhria
authors.forename: D L
authors.initials: DL
authors.lastname: Woodward
authors.forename: W M
authors.initials: WM
authors.lastname: Johnson
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000276:immunology; D001434:Bacteriophage Typing / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000145:classification / Q000276:immunology; D015151:Immunoblotting; D012475:Salmonella / Q000145:classification* / Q000276:immunology / Q000821:virology*; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial