title: Structural changes in native membrane proteins monitored at subnanometer resolution with the atomic force microscope: a review.
abstract: Three membrane proteins, OmpF porin from Escherichia coli, bacteriorhodopsin from Halobacterium salinarium, and the hexagonally packed intermediate (HPI) layer from Deinoccocus radiodurans, were investigated with the atomic force microscope in buffer solution. A resolution of up to 0.8 nm allowed structural differences of individual proteins to be detected. OmpF porin exhibits different static conformations on the outer surface, which possibly represent the two conductive states of the ion channels. Reversible structural changes in the cytoplasmic surface of purple membrane have been induced by changing the force applied to the scanning stylus: doughnut-shaped bacteriorhodopsin trimers transformed into a structure with three pronounced protrusions when the force was reduced from 300 to 100 pN. Furthermore, individual pores of the inner surface of the HPI layer were observed to switch from an "open" to a "closed" state. Together, the structural changes in proteins monitored under physiological conditions suggest that direct observation of function-related conformational changes of biomolecules with the atomic force microscope is feasible.
authors.affiliation: Biozentrum, M.E. Müller Institute for Microscopy, Basel, Switzerland.
authors.forename: D J
authors.initials: DJ
authors.lastname: Müller
authors.forename: C A
authors.initials: CA
authors.lastname: Schoenenberger
authors.forename: F
authors.initials: F
authors.lastname: Schabert
authors.forename: A
authors.initials: A
authors.lastname: Engel
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000648:ultrastructure*; D001436:Bacteriorhodopsins / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D008565:Membrane Proteins / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D011487:Protein Conformation; D018717:Purple Membrane / Q000648:ultrastructure
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D001436:Bacteriorhodopsins