OHIO-1 beta-lactamase mutants: Asp179Gly mutation confers resistance to ceftazidime.
The Asp179Gly mutant of the OHIO-1 beta-lactamase, an SHV enzyme, was constructed to investigate the effect of disruption of the omega loop on beta-lactamase activity in this class A enzyme. In Escherichia coli DH5 alpha the strain possessing the Asp179Gly mutation of the OHIO-1 beta-lactamase demonstrated increased susceptibility to all beta-lactams except ceftazidime and ceftriaxone. The minimum inhibitory concentrations for ceftazidime and ceftriaxone increased from 0.25 and 0.015 microgram/ml to 4.0 and .25 micrograms/ ml respectively. For ceftazidime, a substrate not hydrolyzed by the wild-type enzyme (K(m) > or = 500 microM), the K(m) of the Asp179Gly mutant beta-lactamase was measured to be 7 microM and the Vmax was 0.13 microM/min. The minimum inhibitory concentrations, K(m), and Vmax for all other beta-lactams decreased. Our analysis of this OHIO-1 beta-lactamase mutant suggests that disruption of the salt bridge in the omega loop by substitution of a glycine at position 179 markedly decreases the catalytic efficiency of the enzyme.
Bonomo
R A
RA
Department of Veterans Affairs Medical Center, Department of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA. rab14@po.cwru.edu
Rudin
S D
SD
Shlaes
D M
DM
D001224:Aspartic Acid / Q000502:physiology*; D002442:Ceftazidime / Q000494:pharmacology*; D018550:Cephalosporin Resistance / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010406:Penicillins / Q000494:pharmacology; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics*
D002511:Cephalosporins; D010406:Penicillins; D001224:Aspartic Acid; D002442:Ceftazidime; D001618:beta-Lactamases