title: Reduction in the amide hydrogen exchange rates of an anti-lysozyme Fv fragment due to formation of the Fv-lysozyme complex.
abstract: The Fv fragment of the monoclonal antibody D1.3 was expressed in bacteria. Standard triple resonance techniques were used to obtain the NMR resonance assignments for 211 out of 215 backbone 15N/NH atoms for D1.3 Fv. Using these assignments, hydrogen exchange rates are measured for 82 amide hydrogen atoms in D1.3 Fv free and bound to hen egg-white lysozyme. Upon binding to antigen, exchange rates are decreased for residues throughout the Fv. Many of these residues are located remote from the site of interaction with the antigen. These changes are larger than previously observed for the antigen portion of the complex. Evidently, the beta-sheet structure of the Fv propagates the effects of binding more efficiently than the antigen. These effects are compared between the three different polypeptide chains that make up the complex. These data suggest that reduced dynamics are a general feature of antibody binding to antigen.
authors.affiliation: Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA.
authors.forename: D C
authors.initials: DC
authors.lastname: Williams
authors.forename: G S
authors.initials: GS
authors.lastname: Rule
authors.forename: R J
authors.initials: RJ
authors.lastname: Poljak
authors.forename: D C
authors.initials: DC
authors.lastname: Benjamin
mesh_terms: D000577:Amides / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000276:immunology / Q000378:metabolism*; D001974:Bromosuccinimide / Q000494:pharmacology; D004529:Egg White; D006859:Hydrogen; D007128:Immunoglobulin Fragments / Q000276:immunology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009113:Muramidase / Q000276:immunology / Q000378:metabolism*; D010084:Oxidation-Reduction; D011487:Protein Conformation
chemical_list: D000577:Amides; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007128:Immunoglobulin Fragments; C055966:immunoglobulin Fv; D006859:Hydrogen; D009113:Muramidase; D001974:Bromosuccinimide