Cloning and expression of the two genes coding for L-serine dehydratase from Peptostreptococcus asaccharolyticus: relationship of the iron-sulfur protein to both L-serine dehydratases from Escherichia coli.
The structural genes sdhA and sdhB, coding for the alpha- and beta-subunits of the [4Fe-4S] cluster containing L-serine dehydratase from Peptostreptococcus asaccharolyticus, have been cloned and sequenced. Expression of modified sdhB together with sdhA in Escherichia coli led to overproduction of active His6-tagged L-serine dehydratase. E. coli MEW22, deficient in the L-serine dehydratase L-SD1, was complemented by this sdhBA construct. The derived amino acid sequence of SdhBA shares similarities with both monomeric L-serine dehydratases, L-SD1 and L-SD2, from E. coli and with a putative L-serine dehydratase from Haemophilus influenzae, which suggests that these three enzymes are also iron-sulfur proteins.
Hofmeister
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Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität Marburg, Germany.
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Buckel
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology*; D005420:Flavoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D005816:Genetic Complementation Test; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D012695:L-Serine Dehydratase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010462:Peptostreptococcus / Q000201:enzymology* / Q000235:genetics; D021122:Protein Subunits; D017386:Sequence Homology, Amino Acid; D013385:Succinate Dehydrogenase
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005420:Flavoproteins; D007506:Iron-Sulfur Proteins; D021122:Protein Subunits; C084271:SdhA protein, Bacteria; C108095:SDHB protein, human; D013385:Succinate Dehydrogenase; D012695:L-Serine Dehydratase