[Synthesis of beta-maltosides, derivatives of p-nitrophenol, 2-chloro-4-nitrophenol, and 4-methylumbelliferone, and their use as substrates for determining alpha-glucosidase activity].
Synthesis of beta-maltosides, p-nitrophenyl-beta-D-maltoside and 4-methylumbelliferyl-beta-D-maltoside, based on interaction of hepta-acetate-beta-D-maltosyl fluoride with the corresponding trimethylsilyl ethers of p-nitrophenol and 4-methylumbelliferone is described. 2-Chloro-4-nitrophenyl-beta-D-maltoside was synthesized by interaction of hepta-acetate-alpha-D-maltosyl bromide with 2-chloro-4-nitrophenol in two phase system using phase transfer catalyst. The method of assay of neutral alpha-glucosidase from human kidney and urine using synthesized beta-maltosides (p-nitrophenyl-beta-D-maltoside, 2-chloro-4-nitrophenyl-beta-D-maltoside and 4-methylumbelliferyl-beta-D-maltoside) as substrates and beta-glucosidase as an auxiliary enzyme is proposed. The method is simple, convenient and 10-fold more sensitive than the commonly used alpha-glucosidase assay procedure with the corresponding synthetic alpha-glucosides, p-nitrophenyl-alpha-D-glucoside and 4-methylumbelliferyl-alpha-D-glucoside. A modification of the method, with p-nitrophenyl-beta-D-maltoside as substrate, was applied to the semi-automatic assay of urinary alpha-glucosidase in 96-well microtitre plates.
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D005960:Glucosides / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D006923:Hymecromone / Q000138:chemical synthesis* / Q000737:chemistry; D007668:Kidney / Q000201:enzymology; D008722:Methods; D009596:Nitrophenols / Q000138:chemical synthesis* / Q000737:chemistry; D000520:alpha-Glucosidases / Q000378:metabolism* / Q000652:urine
D005960:Glucosides; D009596:Nitrophenols; D006923:Hymecromone; C068311:4-chloro-2-nitrophenol; D000520:alpha-Glucosidases; C024836:4-nitrophenol
Sintez beta-mal'tozidov, proizvodnykh p-nitrofenola, 2-khlor-4-nitrofenola i 4-metilumbelliferonea, i ikh ispol'zovanie v kachestve substratov dlia opredeleniia aktivnosti alpha-gliukozidazy.