Immunolocalization of glutathione-peroxidase (GSH-PO) in the rat ventral prostate: effects of castration and administration of testosterone.
Immunolocalization of glutathione-peroxidase (GSH-PO) in the rat ventral prostate was studied in the presence and absence of androgen. Male Sprague-Dawley rats were divided into four experimental groups. Group 1 consisted of intact controls. In group 2, rats were sacrificed two days after castration. In groups 3 and 4, rats were injected subcutaneously with 1 mg of testosterone-propionate daily, for three or seven days, beginning two days after castration. The intensity of GSH-PO staining in the glandular epithelial cells of the ventral prostate decreased after castration, but recovered following treatment with testosterone. Furthermore, the prostatic GSH-PO mRNA levels were diminished in the castrated rat ventral prostate but greatly increased by testosterone. These findings strongly suggest that the expression of GSH-PO in the glandular epithelial cells of the rat ventral prostate is dependent on testosterone.
Murakoshi
M
M
Safety Research Department, Teikoku Hormone Mfg. Co., Ltd., Kawasaki, Japan.
Ikeda
R
R
Tagawa
M
M
Takekoshi
S
S
Watanabe
K
K
D000818:Animals; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009919:Orchiectomy; D011467:Prostate / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000502:physiology; D013739:Testosterone / Q000494:pharmacology
D012333:RNA, Messenger; D013739:Testosterone; D005979:Glutathione Peroxidase