Hypothermic ischaemia of the liver: a re-perfusion phenomenon.
BACKGROUND
The effects of hypothermic injury to the liver were investigated on an isolated perfusion circuit by comparing porcine livers with varying degrees of preservation injury.


METHODS
A group of unstored livers (n = 5) were compared to livers stored in University of Wisconsin (UW) solution for 18 h (n = 5), and a group of livers stored in Hartmann's solution for 18 h (n = 5).


RESULTS
We observed that the degree of platelet sequestration was directly related to the severity of the preservation injury. After 2 h of isolated liver perfusion, the perfusate platelet count fell from 148 +/- 14 x 10(9)/L to 84 +/- 13 x 10(9)/L for control livers. In comparison for livers stored in UW solution, the platelet count fell from 173 +/- 43 x 10(9)/L to 61 +/- 14 x 10(9)/L representing a 64.8% fall, while for those stored in Hartmann's solution, an even more profound fall from 152 +/- 36 x 10(9)/L to 19 +/- 9 x 10(9)/L (87.5% fall) was observed. The difference between the UW-stored and Hartmann's-stored livers was significant (P < 0.05). However, using this model, the degree of leukocyte sequestration did not differentiate the groups. Both histological and ultrastructural examination of liver biopsies taken immediately following revascularization demonstrated that for mild degrees of preservation injury following hypothermic storage, changes occur to the sinusoidal lining cells well before changes to the parenchymal elements.


CONCLUSIONS
These findings substantiate the hypothesis that the primary injury associated with hypothermia involves the sinusoidal lining cells (non-parenchymal elements), that it is predominantly a reperfusion phenomenon and that efforts at improving preservation should therefore be targeted primarily at these cells and not the hepatocytes.
Bell
R
R
Department of Surgery, University of Western Australia, Australia.
Makin
G
G
Robbins
P
P
Robertson
T
T
House
A K
AK
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001706:Biopsy; D015925:Cryopreservation / Q000379:methods*; D004195:Disease Models, Animal; D005978:Glutathione; D007328:Insulin; D007552:Isotonic Solutions; D008099:Liver; D008111:Liver Function Tests; D009926:Organ Preservation / Q000009:adverse effects* / Q000379:methods*; D019188:Organ Preservation Solutions; D010976:Platelet Count; D011887:Raffinose; D015427:Reperfusion Injury / Q000097:blood / Q000209:etiology* / Q000473:pathology; D000077325:Ringer's Lactate; D013552:Swine
D007328:Insulin; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D000077325:Ringer's Lactate; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose