Saccharide anions as inhibitors of the malaria parasite.
The asexual erythrocytic stage of Plasmodium falciparum was grown in culture in the presence or absence of glycoconjugate polyanions of varying structure, size and substitutions. Heparin, dextran sulfate, fucoidan and pentosan polysulfate had antimalarial IC50 values between one and 11 microg ml(-1). Constituent heparin disaccharides were ineffective against the malaria parasite and desulfation from either the O- or N-substitution sites of heparin or reduction of the uronic acid carboxyl group neutralized the antimalarial response to varying degrees. Immobilization of heparin onto agarose beads still permitted antimalarial activity suggesting that parasite uptake of the glycoconjugate is not required for inhibition. Accordingly, it is concluded that invasion of free parasites into the erythrocytes was inhibited rather than parasite maturation within the red cell. Merozoite surface antigen-1 was apparently prevented from binding to human erythrocytes in the presence of highly sulfated polyanions and, in a dose-dependent fashion, heparin.
Clark
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Department of Biochemistry and Molecular Biology, Georgetown University, Washington, DC 20007, USA.
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Davidson
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D000818:Animals; D000838:Anions; D016264:Dextran Sulfate / Q000737:chemistry / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020066:Merozoite Surface Protein 1; D010426:Pentosan Sulfuric Polyester / Q000737:chemistry / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D011498:Protein Precursors / Q000378:metabolism; D015800:Protozoan Proteins / Q000378:metabolism
D000838:Anions; D020066:Merozoite Surface Protein 1; D011134:Polysaccharides; D011498:Protein Precursors; D015800:Protozoan Proteins; D010426:Pentosan Sulfuric Polyester; D006493:Heparin; D016264:Dextran Sulfate; C007789:fucoidan