ATR-FTIR spectroscopic investigation of E. coli transconjugants beta-lactams-resistance phenotype.
Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transconjugants TEM-1, TEM-2, TEM-3, SHV-2, SHV-3, SHV-4. A good discrimination of the susceptible strain from the transconjugants was obtained. Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM-2 and SHV-3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to beta-lactamase point-mutations.
Bouhedja
W
W
Laboratoire de Spectroscopie Biomoléculaire, IFR 53, UFR de Pharmacie,Université de Reims Champagne-Ardenne, France.
Sockalingum
G D
GD
Pina
P
P
Allouch
P
P
Bloy
C
C
Labia
R
R
Millot
J M
JM
Manfait
M
M
D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D047090:beta-Lactams / Q000494:pharmacology*
D047090:beta-Lactams