title: S-layer stabilized solid support lipid bilayers.
abstract: This work describes composite structures composed of lipid bilayer or tetraetherlipid monolayer films attached to solid supports with associated crystalline bacterial cell surface layers (S-layers). The bilayer system was established by making use of the strong chemisorption of a first monolayer of thiolipids (1-octadecanethiol or 1,2-dimyristoyl-sn-glycero-3-phosphothioethanol) on gold and attaching a second monolayer of 1,2-dipalmitoyl-sn-3-phosphatidylethanolamine by the Langmuir Schaefer technique. The tetraetherlipid monolayer was composed of Glycerol-dialkyl-nonitol tetraetherlipid (GDNT). The monolayer of GDNT exhibits the thickness of a bilayer with hydrophilic headgroups on both sides and a hydrophobic inner part. Isolated S-layer protein from Bacillus sphaericus (CCM2177, which was injected into the subphase of an LB-trough, recrystallized into a coherent monolayer at the solid supported phospholipid bilayer and at the tetraehtherlipid monolayer. The composite lipid/S-layer structures were stable enough to allow lifting from the air-water interface, rinsing in water, and transfer into a scanning force microscope.
authors.affiliation: Zentrum für Ultrastrukturforschung, Universität für Bodenkultur, Vienna, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Wetzer
authors.forename: D
authors.initials: D
authors.lastname: Pum
authors.forename: U B
authors.initials: UB
authors.lastname: Sleytr
mesh_terms: D001407:Bacillus; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D004075:Diglycerides / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D008051:Lipid Bilayers; D008562:Membrane Glycoproteins / Q000737:chemistry*; D018625:Microscopy, Atomic Force
chemical_list: D001426:Bacterial Proteins; D004075:Diglycerides; D008051:Lipid Bilayers; D008562:Membrane Glycoproteins; C055764:S-layer proteins; C085775:glycerol dialkyl nonitol tetraethyl ether