Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice.
The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.
Nilsson
H
H
Lund University Medical Laser Centre, Lund Institute of Technology, Sweden.
Johansson
J
J
Svanberg
K
K
Svanberg
S
S
Jori
G
G
Reddi
E
E
Segalla
A
A
Gust
D
D
Moore
A L
AL
Moore
T A
TA
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D002338:Carotenoids / Q000378:metabolism*; D005354:Fibrosarcoma / Q000737:chemistry / Q000175:diagnosis; D008099:Liver / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011166:Porphyrins / Q000378:metabolism*; D012513:Sarcoma, Experimental / Q000737:chemistry / Q000175:diagnosis*; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution
D014408:Biomarkers, Tumor; D011166:Porphyrins; C090538:trimethoxycarotenoporphyrin; C090537:trimethylcarotenoporphyrin; D002338:Carotenoids