Expression of soluble, active human macrophage colony stimulating factor in Escherichia coli.
Human macrophage colony stimulating factor (M-CSF) has been successfully overexpressed in Escherichia coli AD494 (DE3) with an expression level of approximate 26% of the total cellular proteins. The truncated human M-CSF gene encoding the amino-terminal 149 amino acids was subcloned into the prokaryotic expression vector pET11d under the control of the inducible T7 promoter. Nearly 40% of the recombinant protein was in the soluble fraction which showed obvious stimulating effects on mouse macrophage colony formation and had an M-CSF specific activity of approximately 1 x 10(6) units/mg soluble protein.
Jiang
X
X
Department of Biochemistry, Nanjing University, P.R. China.
Zhou
A
A
Cen
B
B
Qiu
Q
Q
Dong
X
X
Xu
X
X
D000818:Animals; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology
D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor