Visualization of Golgi apparatus in methacrylate embedded conifer embryo tissue using the monoclonal antibody JIM 84.
Methacrylate embedding followed by resin removal has been used for the first time to visualize a membrane-associated antigen at the tissue level. Monoclonal antibody JIM 84 was used to stain the Golgi apparatus of gymnosperm (conifer) embryos by light microscope immunocytochemistry. Specificity of labelling was confirmed by electron microscope immunocytochemistry using LR-white resin. GA staining was evident in all stages of white spruce somatic embryo development from immature to mature. Some regions of the somatic embryos (e.g. root cap/suspensor region) stained more vigorously than other regions (hypocotyl/cotyledon end). GA also stained in roots of Monterey pine and Douglas fir. Unlike the situation in most angiosperms, JIM 84 antigen appears to be absent from the conifer plasma membrane. However, it appears to be present in representatives of both major classes of higher plants.
Evans
D E
DE
Department of Biology, University of Saskatchewan, Saskatoon, Canada.
Clay
P J
PJ
Attree
S
S
Fowke
L C
LC
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000918:Antibody Specificity; D015153:Blotting, Western; D006056:Golgi Apparatus / Q000276:immunology* / Q000648:ultrastructure; D008689:Methacrylates; D016253:Microscopy, Immunoelectron; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology*; D012639:Seeds / Q000276:immunology / Q000648:ultrastructure; D016610:Tissue Embedding / Q000379:methods; D014197:Trees
D000911:Antibodies, Monoclonal; D008689:Methacrylates; D010940:Plant Proteins