Direct preparation of cyclodextrin monophosphates.
Aqueous solutions of cyclodextrins and inorganic metaphosphate at pH 4, upon drying and subsequent warming, produce mixtures of isomeric monophosphate esters which are amenable to separation by anion-exchange chromatography. The products are characterised by enzymatic, mass, and NMR spectroscopic analysis. The methodology provides a route to these derivatives by a single reaction.
Tarelli
E
E
Joint Microbiology Research Unit, Kings College School of Medicine and Dentistry, London, UK. rcbm800@kcl.ac.uk
Lemercinier
X
X
Wheeler
S
S
D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D003505:Cyclodextrins / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010710:Phosphates / Q000138:chemical synthesis* / Q000737:chemistry; D010757:Phosphorous Acids / Q000737:chemistry; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D010710:Phosphates; D010757:Phosphorous Acids; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex; C043639:metaphosphoric acid; C032613:alpha-cyclodextrin