Potential deleterious effect of anti-viral cytotoxic lymphocyte through the CD95 (FAS/APO-1)-mediated pathway during chronic HIV infection.
The potential deleterious effect through a CD95-based pathway of anti-viral cytotoxic lymphocyte (CTL) during HIV-infection was studied. The present paper reports that a Nef specific CTL line derived from an HIV-infected person is able to kill not only Nef-expressing target cells but also CD95+ compliant Jurkat cells. The two mechanisms of cytotoxicity, i.e. perforin-vs-CD95-dependent were differentiated according to their respective Ca(2+)-dependence. The existence of the dual killing machinery in the anti-HIV CTL line was correlated with the coexpression in these cells of perforin and CD95-L molecules. A model of AIDS pathogenesis involving the deleterious effect through the CD95 pathway of the viral specific CTL response is discussed.
Garcia
S
S
Unitè d'Oncologie Virale, Institut Pasteur, Paris, France.
Fevrier
M
M
Dadaglio
G
G
Lecoeur
H
H
Riviere
Y
Y
Gougeon
M L
ML
D000938:Antigen-Presenting Cells / Q000276:immunology; D002118:Calcium / Q000276:immunology; D002460:Cell Line; D002461:Cell Line, Transformed; D002908:Chronic Disease; D003601:Cytotoxicity Tests, Immunologic; D053222:Fas Ligand Protein; D015693:Gene Products, nef / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D019169:Jurkat Cells; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D019014:fas Receptor / Q000276:immunology*; D054311:nef Gene Products, Human Immunodeficiency Virus
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D015693:Gene Products, nef; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D019014:fas Receptor; D054311:nef Gene Products, Human Immunodeficiency Virus; D054353:Perforin; D002118:Calcium