title: The Saccharomyces cerevisiae acetyl-coenzyme A synthetase encoded by the ACS1 gene, but not the ACS2-encoded enzyme, is subject to glucose catabolite inactivation.
abstract: In Saccharomyces cerevisiae, the structural genes ACS1 and ACS2 each encode an isoenzyme of acetyl-CoA synthetase (ACS; EC 6.2.1.1). Involvement of glucose catabolite repression in regulation of the two isoenzymes was investigated by following ACS activity after glucose pulses (100 mM) to ethanol-limited chemostat cultures. In wild-type S. cerevisiae and in an isogenic strain in which ACS2 had been disrupted, ACS activity decreased after a glucose pulse. No such inactivation was observed in a strain in which ACS1 was disrupted. Western blots demonstrated that the ACS1 product, but not the ACS2 product, was degraded after a glucose pulse. Inactivation kinetics of the ACS1 product resembled those of isocitrate lyase.
authors.affiliation: Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: de Jong-Gubbels
authors.forename: M A
authors.initials: MA
authors.lastname: van den Berg
authors.forename: H Y
authors.initials: HY
authors.lastname: Steensma
authors.forename: J P
authors.initials: JP
authors.lastname: van Dijken
authors.forename: J T
authors.initials: JT
authors.lastname: Pronk
mesh_terms: D000106:Acetate-CoA Ligase / Q000235:genetics / Q000378:metabolism*; D004794:Enzyme Repression / Q000187:drug effects*; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005800:Genes, Fungal; D005947:Glucose / Q000494:pharmacology*; D007522:Isocitrate Lyase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D006597:Fructose-Bisphosphatase; D007522:Isocitrate Lyase; D000106:Acetate-CoA Ligase; D005947:Glucose