title: Preparation of active Cdc7/Dbf4 kinase from yeast cells.
authors.affiliation: Braun Laboratories, California Institute of Technology, Pasadena 91125, USA.
authors.forename: W J
authors.initials: WJ
authors.lastname: Dixon
authors.forename: J L
authors.initials: JL
authors.lastname: Campbell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D016367:Baculoviridae; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000302:isolation & purification*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002846:Chromatography, Affinity; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000096:biosynthesis / Q000302:isolation & purification; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011494:Protein Kinases / Q000096:biosynthesis* / Q000302:isolation & purification*; D017346:Protein Serine-Threonine Kinases; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D018411:Spodoptera; D014162:Transfection / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D017931:DNA Primers; C088096:Dbf4 protein, S cerevisiae; D005656:Fungal Proteins; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C489404:CDC7 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases