title: Pretreatment of clinical specimens with sodium dodecyl (lauryl) sulfate is not suitable for the mycobacteria growth indicator tube cultivation method.
abstract: When using the Mycobacteria Growth Indicator Tube (MGIT), pretreatment of clinical specimens with N-acetyl-L-cysteine-NaOH is recommended by the manufacturer. Processing of clinical specimens (n = 1,000) with sodium dodecyl (lauryl) sulfate-NaOH resulted in both poor recovery and delayed mean time to detection of acid-fast bacilli. Values were comparable to those obtained on solid media.
authors.affiliation: Swiss National Center for Mycobacteria, Department of Medical Microbiology, University of Zurich.
authors.forename: G E
authors.initials: GE
authors.lastname: Pfyffer
authors.forename: H M
authors.initials: HM
authors.lastname: Welscher
authors.forename: P
authors.initials: P
authors.lastname: Kissling
mesh_terms: D001431:Bacteriological Techniques; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*
chemical_list: D012967:Sodium Dodecyl Sulfate