Non-transferrin-bound iron and tumor cells.
The study of iron uptake from low molecular weight complexes by Ehrlich carcinoma cells shows concentration-dependence, and ATP increases the iron uptake from citrate and lactate complexes. Blood proteins can act as inhibitors, and deferoxamine chelation of cell-bound iron complex indicates that the percentage of iron penetrating the cell is about the same for a wide range of iron complex concentrations in the incubation medium (about 5% for ferric lactate). Ascorbic acid increases iron uptake and simultaneously decreases lipid peroxidation. Electrophoresis shows a very high iron transfer from ferric lactate to ATP, and to a lesser extent to ADP and AMP. In the pathological evolution of iron overload to a neoplasia, the probable involvement of an iron exchange between iron complexes from non-transferrin-bound iron of plasma and ATP is discussed.
Anghileri
L J
LJ
Biophysics Laboratory, University of Nancy, France.
Thouvenot
P
P
D000227:Adenine Nucleotides / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002951:Citrates / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D005293:Ferritins / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism*
D000227:Adenine Nucleotides; D002951:Citrates; D005290:Ferric Compounds; D007502:Iron Chelating Agents; D007773:Lactates; D008054:Lipid Peroxides; D012709:Serum Albumin; D014168:Transferrin; D000255:Adenosine Triphosphate; D005293:Ferritins; D007501:Iron; D005978:Glutathione; D001205:Ascorbic Acid