Activity of class I and II isoenzymes of alcohol dehydrogenase measured by a fluorometric method in the sera of patients with obstructive jaundice.
Using new fluorogenic substrates, we measured the activity of class I and II alcohol dehydrogenase (ADH) isoenzymes in the sera of patients with obstructive jaundice. The activity of class I isoenzymes was elevated two-fold, whereas that of class II isoenzymes was unchanged. This increase of class I isoenzymes explains low increase of total serum ADH activity. ADH isoenzymes and total enzyme activities correlated better with aminotransferases, than with alkaline phosphatase and gamma-glutamyltransferase, but we can conclude that class I ADH isoenzymes measured with fluorogenic substrates are indicative of obstructive jaundice.
Chrostek
L
L
Department of Biochemical Diagnostics, Medical University, Białystok, Poland.
Szmitkowski
M
M
D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D000426:Alcohol Dehydrogenase / Q000097:blood* / Q000145:classification; D000469:Alkaline Phosphatase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D002779:Cholestasis / Q000097:blood / Q000201:enzymology*; D005069:Evaluation Studies as Topic; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000145:classification; D008297:Male; D008875:Middle Aged; D009603:Nitroso Compounds; D013050:Spectrometry, Fluorescence / Q000379:methods*; D005723:gamma-Glutamyltransferase / Q000097:blood
D005456:Fluorescent Dyes; D007527:Isoenzymes; D009603:Nitroso Compounds; C008651:4-nitrosodimethylaniline; D000426:Alcohol Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin