Estrogen receptor mRNA in mineralized tissues of rainbow trout: calcium mobilization by estrogen.
RT-PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss. The rainbow trout estrogen receptor (ER)-specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a 32P-labelled rtER probe under stringent conditions. These data provide the first in vivo evidence of ER mRNA in bone and scale tissues of rainbow trout and suggest that the effects of estrogen observed in this study (increased bone mineral and decreased scale mineral contents, respectively) may be mediated directly through ER.
Armour
K J
KJ
Department of Animal and Plant Sciences, University of Sheffield, UK. K.Armour@rri.sari.ac.uk
Lehane
D B
DB
Pakdel
F
F
Valotaire
Y
Y
Graham
R
R
Russell
R G
RG
Henderson
I W
IW
D000818:Animals; D015152:Blotting, Northern; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics
D010710:Phosphates; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D004958:Estradiol; D002118:Calcium