Identification of surface tegumental antigens of normal and irradiated schistosomula.
Sodium dodecyl sulphate- poly acrylamide gel electronphoresis (SDS-PAGE) fractionation of tegumental surface antigens (STEG-Ags.) of 7-day cultured normal and irradiated schistosomula showed no obvious qualitative differences. The observed polypeptide bands of both normal irradiated STEG-Ags. were almost identical and have similar corresponding molecular weights. The immunoblotting assay, using different types of mouse sera, revealed similarity between the bands of both normal and irradiated STEG-Ags. recognized by each type of mouse serum. No qualitative rather than quantitative differences have been observed. The quantitative differences were reflected in intensively staining of some bands from normal STEG-Ag. rather than their corresponding bands of the same molecular weights from irradiated STEG-Ag.
Charmy
R A
RA
Faculty of Science, Cairo University, Egypt.
el-Kashef
H S
HS
el Ghorab
N M
NM
Gad
H S
HS
D000818:Animals; D000909:Antibodies, Helminth; D000947:Antigens, Helminth / Q000032:analysis* / Q000528:radiation effects; D000954:Antigens, Surface / Q000032:analysis* / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D012550:Schistosoma mansoni / Q000737:chemistry* / Q000528:radiation effects*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface