Superovulation induction in the house musk shrew (Suncus murinus).
The number of ovulated oocytes in the adult house musk shrews was examined by natural mating. The positive rate of ovulation was 83.3%, and the mean number of ovulated oocytes was 4.4 +/- 1.9 (Mean +/- SD). The induction of superovulation was examined by administering either pregnant mare's serum gonadotropin (PMSG) or human chorionic gonadotropin (hCG), exogenous gonadotropin, to house musk shrews. As a result, the number of ovulated oocytes were within the number of oocytes ovulated spontaneously. The method of superovulation induction by administering both PMSG and hCG to house musk shrews was investigated. In the group intraperitoneally with 7.5 i.u. of PMSG followed by 7.5 i.u. of hCG 48 hr later, the positive rate of ovulation was as high as 94.6% and the mean number of ovulated oocytes was 11.7 +/- 13.4. Moreover, in 15 out of these 35 animals positive for ovulation, 10 oocytes or more were observed and the mean number of ovulated oocytes was 21.0 +/- 16.5, showing the superovulatory response. In the group of 5.0 i.u. PMSG and 5.0 i.u. hCG injected at an interval of 72 hr, every animal was induced to ovulate and the mean number of ovulated oocytes was 41.3 +/- 19.9. Thus, the injection of PMSG and hCG at a longer interval increased the number of superovulated oocytes. Furthermore, 97.5% of eggs recovered from superovulating animals contained cumulus cells.
Matsuzaki
T
T
Division of Laboratory Animal Resources, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
Matsuzaki
K
K
Yokoyama
M
M
Yamada
S
S
Saito
M
M
D000818:Animals; D002452:Cell Count; D006063:Chorionic Gonadotropin / Q000008:administration & dosage*; D005260:Female; D006064:Gonadotropins, Equine / Q000008:administration & dosage*; D008297:Male; D009865:Oocytes; D006080:Ovarian Follicle / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D012788:Shrews / Q000502:physiology*; D013480:Superovulation
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine