title: Identification of a biological inactive complex form of pokeweed antiviral protein.
abstract: Pokeweed antiviral protein (PAP) inactivates both eukaryotic and prokaryotic ribosomes via a specific depurination of rRNA. The sensitivity of pokeweed ribosomes to PAP implies the existence of a mechanism to protect the plant. Using monoclonal antibodies specific to PAP, a protein complex (PAPi) which contained PAP was identified in leaf extract. In this complex, the enzymatic activity of the toxin was strongly inhibited. This protein complex had a pI lower than that of PAP and was separated from free PAP by a preparative native gel electrophoresis. PAPi had an apparent molecular mass of 57 kDa and was dissociated by heating for 5 min at 80 degrees C or by treatment by alkaline or acidic pH or by 7 M urea. The other components involved in the complex remain unknown.
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire, UFR Sciences et Techniques, Besançon, France.
authors.forename: B
authors.initials: B
authors.lastname: Desvoyes
authors.forename: J L
authors.initials: JL
authors.lastname: Poyet
authors.forename: J L
authors.initials: JL
authors.lastname: Schlick
authors.forename: P
authors.initials: P
authors.lastname: Adami
authors.forename: M
authors.initials: M
authors.lastname: Jouvenot
authors.forename: P
authors.initials: P
authors.lastname: Dulieu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000032:analysis* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000032:analysis* / Q000494:pharmacology; D010944:Plants / Q000737:chemistry; D011489:Protein Denaturation; D054789:Ribosome Inactivating Proteins, Type 1
chemical_list: D000998:Antiviral Agents; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein