Nature's motility blockers: controlling human sperm motility machinery from the outside. Chemical characterization of a peritoneal fluid lipid that induces sperm immobilization.
A molecule isolated from the peritoneal fluids of women undergoing laparoscopy for in-vitro fertilization techniques has been chemically characterized and identified as 1-palmitic-3-phosphorylcholine (lysophosphatidylcholine, LPC). This lipid is able, at physiological concentrations, to completely inhibit sperm motility in vitro in a dose-dependent way. Synthetic LPC induced rapid and complete arrest of sperm motility when added to sperm suspensions at physiological concentrations without any damage to cell membranes. Taken together, these results suggest that LPC may represent a previously unrecognized in-vivo modulator of human sperm motility.
Keller
F
F
Andrology Laboratory, La Carità Hospital, Locarno, Switzerland.
Togni
G
G
Soldati
G
G
Balmelli
T
T
Medici
G
G
Rose
K
K
Balerna
M
M
D001202:Ascitic Fluid / Q000737:chemistry*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007247:Infertility, Female / Q000209:etiology / Q000503:physiopathology; D008244:Lysophosphatidylcholines / Q000302:isolation & purification* / Q000494:pharmacology*; D008297:Male; D010718:Phosphatidylserines / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology; D011493:Protein Kinase C / Q000502:physiology; D013078:Sperm Immobilizing Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects* / Q000502:physiology*
D008244:Lysophosphatidylcholines; D010718:Phosphatidylserines; D013078:Sperm Immobilizing Agents; D011493:Protein Kinase C