Induction of nuclear factor kappa B/Rel nuclear activity in human peripheral blood T lymphocytes by anti-HLA class I monoclonal antibodies.
Monoclonal antibodies against either monomorphic or polymorphic determinants of class I antigen induced in PBMC and highly purified T lymphocytes the nuclear activity of NF-kappa B/Rel complexes. These included both p50/p50 and p50/p65 dimers, recognized by specific antibodies in EMSA. The induced complexes were detectable in extracts of cells incubated with anti-class I monoclonal antibody (mAb) for 1.5 h; the induction was maximal at 5 h, persistent at 16 h and no longer observed at 40 h. The mAb failed to induce NF-kappa B/Rel nuclear activity in cells incubated in the presence of 3,4-dichloroisocoumarin, an inhibitor of I kappa B-alpha degradation. Together, these results suggest that class I triggering can induce the activity of NF-kappa B/Rel nuclear activity in peripheral blood T lymphocytes, thereby modulating the expression of genes regulated by these transcription factors.
Turco
M C
MC
Department of Biochemistry, University Federico II, Napels.
Romano
M F
MF
Lamberti
A
A
Petrella
A
A
Bisogni
R
R
Sun
S C
SC
Ferrone
S
S
Bonelli
P
P
Cerra
M
M
Venuta
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D019556:COS Cells; D003374:Coumarins / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000502:physiology; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000502:physiology*; D006801:Humans; D049934:Isocoumarins; D007700:Kinetics; D016328:NF-kappa B / Q000096:biosynthesis*; D052002:NF-kappa B p50 Subunit; D009687:Nuclear Proteins / Q000096:biosynthesis; D011817:Rabbits; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D051996:Transcription Factor RelA
D000911:Antibodies, Monoclonal; D003374:Coumarins; D015395:Histocompatibility Antigens Class I; D049934:Isocoumarins; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D009687:Nuclear Proteins; D015842:Serine Proteinase Inhibitors; D051996:Transcription Factor RelA; C046235:3,4-dichloroisocoumarin