title: Induction of IgE antibody responses by glutathione S-transferase from the German cockroach (Blattella germanica).
abstract: We report that a major 23-kDa allergen from German cockroach (Blattella germanica) is a glutathione S-transferase (EC 2.5.1.18; GST). Natural B. germanica GST, purified from cockroach body extracts by glutathione affinity chromatography, and recombinant protein expressed in Escherichia coli using the pET21a vector, showed excellent IgE antibody binding activity. B. germanica GST caused positive immediate skin tests in cockroach-allergic patients using as little as 3 pg of recombinant protein. The NH2-terminal sequence of the natural protein and the deduced amino acid sequence from cDNA were identical except for one substitution (Phe9 --> Cys). Assignment of this protein to the GST superfamily was based on binding to glutathione and sequence identity (42-51%) to the GST-2 subfamily from insects, including Anopheles gambiae and Drosophila melanogaster. B. germanica GST contained 18 of the 26 invariable residues identified in mammalian GST by x-ray crystallography and exhibited enzymic activity against a GST substrate. Our results show that cockroach GST causes IgE antibody responses and is associated with asthma. The data strongly support the view that the immune response to GST plays an important role in allergic diseases.
authors.affiliation: Asthma and Allergic Diseases Center, University of Virginia, Charlottesville, Virginia 22908, USA.
authors.forename: L K
authors.initials: LK
authors.lastname: Arruda
authors.forename: L D
authors.initials: LD
authors.lastname: Vailes
authors.forename: T A
authors.initials: TA
authors.lastname: Platts-Mills
authors.forename: M L
authors.initials: ML
authors.lastname: Hayden
authors.forename: M D
authors.initials: MD
authors.lastname: Chapman
mesh_terms: D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003058:Cockroaches / Q000276:immunology*; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000485:Allergens; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D005982:Glutathione Transferase