Generation of phosphorylated cyclin-dependent kinase 2 and functional characterization of threonine-160-specific phosphatase KAP.
Poon
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Department of Biochemistry, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001345:Autoradiography; D042846:CDC2-CDC28 Kinases; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002846:Chromatography, Affinity; D003001:Cloning, Molecular / Q000379:methods; D051357:Cyclin-Dependent Kinase 2; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D018844:Cyclin-Dependent Kinases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D016213:Cyclins / Q000302:isolation & purification / Q000378:metabolism; D054637:Dual-Specificity Phosphatases; D004926:Escherichia coli; D005982:Glutathione Transferase; D006801:Humans; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D016324:Sequence Tagged Sites; D013379:Substrate Specificity; D013912:Threonine; D014443:Tyrosine
D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins; D010761:Phosphorus Radioisotopes; D011993:Recombinant Fusion Proteins; D013912:Threonine; D014443:Tyrosine; D000255:Adenosine Triphosphate; D005982:Glutathione Transferase; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; C084429:CDKN3 protein, human; D054637:Dual-Specificity Phosphatases; D017027:Protein Tyrosine Phosphatases