Evaluation of transport media for Pasteurella multocida isolates from rabbit nasal specimens.
A suitable medium for the transport of Pasteurella multocida in nasal specimens from rabbits was investigated by using pure cultures of the organism and nasal swabs from infected rabbits. First, the ability of eight transport media to preserve the viabilities of P. multocida strains isolated from rabbits was studied. Cary-Blair medium and Leibovitz medium no. 15 (L-15) were found to be superior to the other six media tested, enabling survival of the organism for more than 14 days at room temperature. Second, the survival of P. multocida in nasal specimens was evaluated on both Cary-Blair medium and L-15. The recovery rate of the organism from these two media was more than 80 to 90% during 4 days of storage and decreased gradually with increasing preservation time. There were no significant differences (P > 0.05) in recovery rates of the organism between Cary-Blair medium and L-15. On the basis of these results, we recommend the use of Cary-Blair medium for the transport of P. multocida in rabbit nasal specimens because of the ease of transport of nasal swabs by mail.
Kawamoto
E
E
Animal Research Center, Tokyo Medical College, Shinjuku, Japan. kawamoto@tokyo-med.ac.jp
Sawada
T
T
Maruyama
T
T
D000818:Animals; D003470:Culture Media; D005069:Evaluation Studies as Topic; D009297:Nasal Mucosa / Q000382:microbiology; D016979:Pasteurella multocida / Q000302:isolation & purification*; D011817:Rabbits; D013045:Species Specificity; D013048:Specimen Handling; D016019:Survival Analysis
D003470:Culture Media