Endothelin-1 and intraocular inflammation in pigmented rabbit eyes.
We assessed the function of Endothelin-1 (ET-1) in the development of anterior chamber inflammation in pigmented rabbit eyes. After the injection of ET-1 solution (10(-13), 10(-11), 10(-9), or 10(-7) M, diluted with 300 microL of artificial aqueous humor) into the anterior chamber, the aqueous protein concentration (APC) increased significantly in a dose-dependent fashion. Peak effects were observed 1-2 hours posttreatment. The APC returned to normal 12 hours after the injection. Pretreatment with antiprostaglandin agents, topical indomethacin, or intravenous diclofenac sodium suppressed the increase in APC. In an endotoxin-induced experimental uveitis model, the ET-1 concentration in the aqueous humor was significantly higher than in normal controls, as was the plasma ET-1 level. These results suggest that ET-1 is an important mediator in ocular inflammatory reactions via arachidonic acid cascade.
Shoji
N
N
Department of Ophthalmology, Musashino Red Cross Hospital, Tokyo, Japan.
Oshika
T
T
Masuda
K
K
D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects / Q000378:metabolism*; D001082:Aqueous Humor / Q000378:metabolism*; D018916:Blood-Aqueous Barrier; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004008:Diclofenac / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000494:pharmacology*; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D007249:Inflammation / Q000209:etiology / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D011817:Rabbits; D014605:Uveitis / Q000139:chemically induced / Q000378:metabolism*
D016861:Cyclooxygenase Inhibitors; D019332:Endothelin-1; D008070:Lipopolysaccharides; D004008:Diclofenac; D007213:Indomethacin