DC-Chol liposome-mediated gene transfer in rat spinal cord.
We examined the potential of non-viral vector-mediated gene transfection in the rat spinal cord. Reporter gene (beta-gal) or brain-derived neurotrophic factor (BDNF) cDNA containing a pCMV promoter complexed with DC-Chol liposomes was injected into the intact rat spinal cord gray matter. RT-PCR confirmed the increased expression of BDNF mRNA in the injection areas. X-gal staining demonstrated the localized expression of beta-gal reporter genes. No overt tissue damage caused by DC-Chol liposome/DNA complex injections was detected. These results suggest that cationic liposome-mediated delivery can be a practical method for gene transfer in spinal cord.
Yang
K
K
Vivian L. Smith Center for Neurologic Research, University of Texas Houston Health Science Center 77030, USA.
Mu
X S
XS
Hayes
R L
RL
Qiu
Y H
YH
Sorgi
F L
FL
Huang
L
L
Clifton
G L
GL
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000378:metabolism; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics*; D008081:Liposomes / Q000494:pharmacology*; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D008081:Liposomes; D012333:RNA, Messenger; C070648:3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; D002784:Cholesterol; D001616:beta-Galactosidase