title: Analysis of non-polar deletion mutations in the genes of the spo0K (opp) operon of Bacillus subtilis.
abstract: The spo0K (opp) operon of Bacillus subtilis encodes an oligopeptide permease that is required for uptake of oligopeptides, development of genetic competence, and initiation of sporulation. We made in-frame, non-polar deletion mutations in each of the first four genes of the five-gene spo0K operon and tested effects on oligopeptide transport, sporulation, and expression of competence genes. spo0KA, B, C, and D were required for sporulation, competence development, and oligopeptide transport. Disruption of spo0KE caused a less severe phenotype than did disruption of any of the other genes of the operon.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: LeDeaux
authors.forename: J M
authors.initials: JM
authors.lastname: Solomon
authors.forename: A D
authors.initials: AD
authors.lastname: Grossman
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D001426:Bacterial Proteins; D001692:Biological Transport; D005798:Genes, Bacterial / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D009842:Oligopeptides / Q000378:metabolism; D009876:Operon / Q000235:genetics; D010453:Peptide Synthases / Q000235:genetics; D017384:Sequence Deletion / Q000502:physiology*; D013171:Spores, Bacterial; D014169:Transformation, Bacterial / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D009842:Oligopeptides; C037230:oligopeptide permease, Bacteria; D010453:Peptide Synthases; C069542:surfactin synthetase