Effect of adrenaline on lymphocyte metabolism and function. A mechanism involving cAMP and hydrogen peroxide.
This study examined the effect of adrenaline on lymphocyte metabolism and function. The following parameters were addressed: cell proliferation, glucose and glutamine metabolism as indicated by the measurement of enzyme activities, the utilization of metabolites and production and oxidation of substrates. We also evaluated the involvement of beta-receptors in this response as well as the possible effect of cAMP and hydrogen peroxide in the process of lymphocyte activation by adrenaline. The results indicated that adrenaline is able to induce metabolic changes in lymphocytes that are related to enhanced proliferative capacity, but under physiological conditions fails to initiate the process, the catecholamine could, increase cell proliferation via increased production of H2O2 by macrophages, since this reactive oxygen intermediate can act as a trigger for lymphocyte activation. The results also showed that distinct populations of lymphocytes present different responses to adrenaline activation, as demonstrated by cells obtained from the same site but exposed to different mitogens such as LPS and ConA.
Rosa
L F
LF
Department of Histology and Embryology, Biomedical Sciences Institute, University of São Paulo, Brasil. costarosa@bmb.icb1.usp.br
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D002950:Citrate (si)-Synthase / Q000187:drug effects / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D005972:Glutaminase / Q000187:drug effects / Q000378:metabolism; D005973:Glutamine / Q000187:drug effects / Q000378:metabolism; D006593:Hexokinase / Q000187:drug effects / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D008070:Lipopolysaccharides; D005973:Glutamine; D003208:Concanavalin A; D003994:Bucladesine; D011433:Propranolol; D006861:Hydrogen Peroxide; D000242:Cyclic AMP; D002950:Citrate (si)-Synthase; D006593:Hexokinase; D005972:Glutaminase; D005947:Glucose; D007545:Isoproterenol; D004837:Epinephrine