title: Unique protein kinase C profile in mouse oocytes: lack of calcium-dependent conventional isoforms suggested by rtPCR and Western blotting.
abstract: rtPCR and Western blotting were used to determine which members of the PKC family are present in both immature and mature mouse oocytes. Using isoform-specific PCR primers and antibodies PKC-delta and -lambda were detected while such techniques failed to observe the conventional isoforms of PKC-alpha, -beta, -gamma. This isoform profile was confirmed using an alternative PCR strategy, which allowed discrimination of PCR products derived from conventional and novel PKC isoforms. In addition PKC-epsilon, -eta, -theta and -zeta were not detected by rtPCR. These results suggest that the predominant isoforms in oocytes are PKC-delta and -lambda.
authors.affiliation: Medical Research Council Experimental Embryology and Teratology Unit, St. George's Hospital Medical School, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: Gangeswaran
authors.forename: K T
authors.initials: KT
authors.lastname: Jones
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D017931:DNA Primers; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase
chemical_list: D017931:DNA Primers; D007527:Isoenzymes; D011493:Protein Kinase C; D012194:RNA-Directed DNA Polymerase; D002118:Calcium