title: Blockade of CD2-LFA-3 interactions protects human skin allografts in immunodeficient mouse/human chimeras.
abstract: A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, human skin grafts show a reproducible pattern of progressive human T-cell infiltration and human graft microvascular injury that resembles human first-set skin graft rejection. Murine Mab to human LFA-3 or human LFA-3-IgG1 fusion protein, but not isotype-matched control antibodies, each markedly protected skin grafts from leukocyte infiltration and injury. These data provide the first evidence that LFA-3 functions in vivo and establish the ability of this new model to test human-specific immune modulators.
authors.affiliation: Molecular Cardiobiology Program, Yale University School of Medicine, New Haven, CT 06536, USA.
authors.forename: P
authors.initials: P
authors.lastname: Sultan
authors.forename: J S
authors.initials: JS
authors.lastname: Schechner
authors.forename: J M
authors.initials: JM
authors.lastname: McNiff
authors.forename: P S
authors.initials: PS
authors.lastname: Hochman
authors.forename: C C
authors.initials: CC
authors.lastname: Hughes
authors.forename: M I
authors.initials: MI
authors.lastname: Lorber
authors.forename: P W
authors.initials: PW
authors.lastname: Askenase
authors.forename: J S
authors.initials: JS
authors.lastname: Pober
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D018801:CD2 Antigens / Q000378:metabolism*; D018968:CD58 Antigens / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D016513:Mice, SCID; D016038:Skin Transplantation / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D018183:Transplantation Chimera / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D000911:Antibodies, Monoclonal; D018801:CD2 Antigens; D018968:CD58 Antigens; D007074:Immunoglobulin G