Pediocin PD-1, a bactericidal antimicrobial peptide from Pediococcus damnosus NCFB 1832.
Pediocin PD-1, produced by Pediococcus damnosus NCFB 1832, is inhibitory to several food spoilage bacteria and food-borne pathogens. However, pediocin PD-1 is not active against other Pediococcus spp. and differs in this respect to other pediocins produced by Pediococcus acidilactici and Pediococcus pentosaceus. Production of pediocin PD-1 starts during early growth and reaches-a plateau at the end of exponential growth. Pediocin PD-1 was partially purified and its size was determined by tricine-SDS-PAGE as approximately 3.5 kDa. The isoelectric point (pI) of pediocin PD-1 is approximately 3.5, as determined with the Rotofor electrofocusing cell (BioRad). Pediocin PD-1 is heat-resistant (10 min at 121 degrees C) and remains active after 30 min of incubation at pH 2-10. Pediocin PD-1 is resistant to treatment with pepsin, papain, alpha-chemotrypsin and trypsin, but not Proteinase K. Pediocin PD-1 is bactericidal against sensitive cells of Oenococcus oeni (previously Leuconostoc oenos).
Green
G
G
Department of Microbiology, University of Stellenbosch, South Africa.
Dicks
L M
LM
Bruggeman
G
G
Vandamme
E J
EJ
Chikindas
M L
ML
D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010450:Endopeptidases; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008970:Molecular Weight; D000070017:Pediocins; D010376:Pediococcus / Q000737:chemistry*
D001430:Bacteriocins; D000070017:Pediocins; C107750:pediocin PD-1; D010450:Endopeptidases