Factors secreted by activated microglia and monocytes reduce amyloidogenesis in vascular smooth muscle cells.
Smooth muscle cells cultured from amyloid-beta-affected arteries accumulate amyloid-beta peptide A beta. We now show that accumulation of "A beta" deposits in this model can be significantly reduced by culture in conditioned media from microglia and monocytes. Reduced A beta accumulation was associated with (i) lower secretion of A beta, (ii) increased secretion, but not cellular levels of amyloid-beta-precursor protein (A beta PP), and (iii) increased cell proliferation and metabolic activity. We suggest that improper regulation of A beta PP metabolism by monokines may facilitate vascular amyloidogenesis.
Frackowiak
J
J
NYS Institute for Basic Research in Developmental Disabilities, Department of Pathological Neurobiology, Staten Island 10314, USA.
Mazur-Kolecka
B
B
Carroll
R T
RT
Chauhan
A
A
Wisniewski
H M
HM
D016564:Amyloid beta-Protein Precursor / Q000378:metabolism* / Q000502:physiology; D000686:Amyloidosis / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004285:Dogs; D007150:Immunohistochemistry; D017628:Microglia / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*
D016564:Amyloid beta-Protein Precursor; D003470:Culture Media