[Isolation and properties of mutants devoid of pseudo-HPr activity of the fructose transfer system in Escherichia coli K12].
Mutants without pseudo-HPr activity intrinsic to the H domain of the FruB protein were obtained in Escherichia coli K12 through insertional mutagenesis by means of the MudIlac phage and TnPhoA transposon. For isolating these mutants, double mutants of enteric bacterium (ptsH fruR of ptsH fruS) were used as original strains. These double mutants were inactive with respect to the total HPr protein of the phosphoenolpyruvate-carbohydrate phosphotransferase system and could not provide constitutive synthesis of fructose-specific proteins of this system. The mutants obtained were designated fruH, mapped, and genetically characterized. Insertions were shown to be located in exactly the portion of the fruB gene that specifies synthesis of the H domain of the FruB protein. The fruH mutations decreased motility of bacterial both in a medium free of carbohydrates and in a medium with glucose, fructose, or maltose. In addition, fruH mutants with the MudIlac genome insertion had sharply decreased activity of beta-galactosidase.
Sergeev
K V
KV
Umiarov
A M
AM
Dobrynina
O Iu
OIu
Bol'shakova
T N
TN
Gershanovich
V N
VN
D001426:Bacterial Proteins / Q000235:genetics*; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D005632:Fructose / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D016254:Mutagenesis, Insertional; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D011494:Protein Kinases; D017434:Protein Structure, Tertiary
D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D047908:Intracellular Signaling Peptides and Proteins; C028640:fructose-induced HPr-like protein, bacteria; D005632:Fructose; D011494:Protein Kinases; C488924:fruB protein, E coli; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System
Vydelenie i svoĭstva mutantov, lishennykh psevdo-HPr aktivnosti sistemy perenosa fruktozy u Escherichia coli K12.