title: A plasmid-mediated CMY-2 beta-lactamase from an Algerian clinical isolate of Salmonella senftenberg.
abstract: Multiresistance to antibiotics including beta-lactams, e.g. cefoxitin, was transferred by conjugation to Escherichia coli strain C1a from a clinical isolate of Salmonella senftenberg recovered from stools of an Algerian child. The susceptibility pattern to beta-lactams was similar to the profile mediated by an AmpC-type beta-lactamase. By biochemical analysis, typical AmpC-type enzyme substrate and inhibition profiles were obtained. Finally, an ampC plasmid-encoded beta-lactamase gene was cloned and sequenced. Its deduced amino acid sequence confirmed its identity as a class C beta-lactamase. It showed 99.5% sequence identity with the plasmid-mediated beta-lactamase CMY-2. The differences in the amino acid sequences of the two enzymes were located in the signal peptide.
authors.affiliation: Laboratoire de Biologie Médicale, Hôpital d'instruction des armées Bégin, Saint-Mandé, France. jlkoeck@filnet.fr
authors.forename: J L
authors.initials: JL
authors.lastname: Koeck
authors.forename: G
authors.initials: G
authors.lastname: Arlet
authors.forename: A
authors.initials: A
authors.lastname: Philippon
authors.forename: S
authors.initials: S
authors.lastname: Basmaciogullari
authors.forename: H V
authors.initials: HV
authors.lastname: Thien
authors.forename: Y
authors.initials: Y
authors.lastname: Buisson
authors.forename: J D
authors.initials: JD
authors.lastname: Cavallo
mesh_terms: D000462:Algeria; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012475:Salmonella / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases