A freeze dried kit formulation for the preparation of 99mTc-EHDP-MoAb-IOR CEA1 complex.
A freeze dried kit formulation for the preparation of 99mTc-labelled IOR-CEA1, a monoclonal antibody (MoAb) against a human colorectal cancer, was developed. Methods of analysis were also established permitting identification of radiochemical impurities which may be present in radiopharmaceutical solution. 99mTc-EHDP-MoAb-IOR CEA1 prepared by the kit method was evaluated in human volunteers to apply the radioimmunodetection of human colon cancer. Data demonstrated that the complex formed after kit-reconstitution shows excellent stability and tumor seeking properties. Colon carcinoma in patients was successfully detected 6-8 and 20-28 h post administration.
Ferro-Flores
G
G
Department of Radioactive Materials, Instituto Nacional de Investigaciones Nucleares, Mexico.
Lezama-Carrasco
J
J
D000230:Adenocarcinoma / Q000000981:diagnostic imaging; D000328:Adult; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000000981:diagnostic imaging; D012968:Etidronic Acid / Q000737:chemistry*; D005612:Freeze Drying; D006801:Humans; D018796:Immunoconjugates; D007553:Isotope Labeling / Q000295:instrumentation*; D015609:Organotechnetium Compounds / Q000737:chemistry*; D011446:Prospective Studies; D011877:Radionuclide Imaging
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D018796:Immunoconjugates; D015609:Organotechnetium Compounds; C019561:technetium Tc 99m etidronate; D012968:Etidronic Acid