An in vitro pharmacological examination of venom from the soldierfish Gymnapistes marmoratus.
The aim of the present study was to commence a characterisation of some of the basic pharmacological properties of venom from the soldierfish (Gymnapistes marmoratus). Soldierfish venom was prepared by extraction into 10% glycerol and centrifugation to remove insoluble material. Protein content was determined and venom concentrations were expressed as microgram venom protein. Soldierfish venom (0.5-15 micrograms/ml) produced concentration-dependent contractile responses in guinea-pig isolated ileum (GPI) and longitudinal smooth muscle (LSM) preparations. The muscarinic receptor antagonist atropine (10 nM) significantly inhibited responses of LSM to soldierfish venom (2.5 micrograms/ml). Responses to soldierfish venom (4-5 micrograms/ml) in GPI were not significantly affected by the ganglion-blocking drug mecamylamine (10 microM) or by incubation with blood cholinesterase. The cyclooxygenase inhibitor indomethacin (2 microM) significantly inhibited responses to soldierfish venom (2.5 micrograms/ml) in LSM. Neither the thromboxane A2/prostaglandin H2 receptor antagonist GR32191B (1 microM) nor the leukotriene receptor antagonist SB205312 (10 nM) significantly affected responses to soldierfish venom (5 micrograms/ml) in GPI. Responses to soldierfish venom (2.5-5 micrograms/ml) were not significantly inhibited by the histamine receptor antagonist mepyramine (0.5 microM), the angiotensin-converting enzyme inhibitor captopril (2 microM) or the neurokinin-1 receptor antagonist CP-99,994 (0.1 microM) in LSM. The angiotensin AT1 receptor antagonist EXP3174 (0.1 microM) also failed to inhibit significantly the responses to soldierfish venom (5 micrograms/ml) in GPI. A fluorometric assay for the detection of 5-hydroxytryptamine (5-HT) and related compounds indicated a level in soldierfish venom of 1.60 +/- 0.01 ng of 5-HT-like substance per microgram venom protein. Soldierfish venom (0.5-10 micrograms/ml) produced concentration-dependent contractile responses in rat isolated stomach fundus strips, and these responses (2.5 micrograms/ml) were significantly inhibited by the 5-HT1/5-HT2 receptor antagonist methysergide (0.1 microM). These results suggest that soldierfish venom may stimulate the release of acetylcholine to act at muscarinic receptors on guinea-pig gastrointestinal smooth muscle. The venom also appears to be causing the release of cyclooxygenase products, such as prostaglandins, and contains 5-HT, or a 5-HT-like substance, that acts directly at 5-HT receptors.
Hopkins
B J
BJ
Department of Pharmacology, Monash University, Clayton, Victoria, Australia.
Hodgson
W C
WC
Sutherland
S K
SK
D000818:Animals; D005397:Fish Venoms / Q000494:pharmacology*; D005400:Fishes, Poisonous; D005748:Gastric Fundus / Q000187:drug effects; D006168:Guinea Pigs; D066298:In Vitro Techniques; D016014:Linear Models; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects; D051381:Rats
D005397:Fish Venoms