Sequence of the VP7 gene of an atypical human rotavirus: evidence for genetic and antigenic drift.
The nucleotide sequence of the gene encoding the outer capsid glycoprotein, VP7, isolated from a reassortant human rotavirus, M3014, was determined. The deduced amino acid sequence exhibited significant identity to the VP7 from a standard strain belonging to serotype G4, although the antigenic regions of the M3014 VP7 resembled sequences from both serotype G4 and G9 viruses. However, reactivity with G4 or G9 serotype-specific monoclonal antibodies was not observed. We suggest that the M3014 VP7 was derived from sequential mutation of a G4-like progenitor gene resulting in a protein with novel antigenic properties.
Palombo
E A
EA
Department of Gastroenterology, Royal Children's Hospital, Parkville, Victoria, Australia.
Bugg
H C
HC
Masendycz
P J
PJ
Bishop
R F
RF
D000595:Amino Acid Sequence; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016865:Reassortant Viruses / Q000235:genetics; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012703:Serotyping
D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus