Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells.
Effects of intraluminal pressure on cultured intestinal epithelial cells were assessed by measuring cell proliferation and DNA synthesis after exposure to various pressures. Pressures of 40 to 120 mm Hg promoted cell proliferation and DNA synthesis. Pressure-induced DNA synthesis was further enhanced by the addition of interleukin-2, suggesting the regulation of intestinal epithelial growth by pressure in coordination with cytokines. Pretreatment with either a phospholipase C inhibitor or protein kinase C inhibitor significantly inhibited DNA synthesis promoted by pressure and interleukin-2. This study demonstrates a novel mechanism whereby pressure regulates cell growth in intestinal epithelial cells, possibly via activation of phospholipase C and protein kinase C.
Hirokawa
M
M
Department of Internal Medicine, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan.
Miura
S
S
Shigematsu
T
T
Yoshida
H
H
Hokari
R
R
Higuchi
H
H
Kurose
I
I
Kimura
H
H
Saito
H
H
Nakaki
T
T
Ishii
H
H
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002219:Carbamates / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D004261:DNA Replication; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D048448:Phenylcarbamates; D010812:Physical Stimulation; D011312:Pressure; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors
D002147:Calmodulin; D002219:Carbamates; D004791:Enzyme Inhibitors; D007376:Interleukin-2; D048448:Phenylcarbamates; D013449:Sulfonamides; C025508:2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate; C017967:W 7; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D010738:Type C Phospholipases