Thin-layer chromatographic detection of ivermectin in cattle dung.
A qualitative method based on thin-layer chromatography (TLC) is described that reliably detects ivermectin as a fluorescent derivative in extracts of cattle dung. The limit of detection (LOD) was < or = 40 ng/g of wet dung. These observations were statistically documented and shown visually using digital imagery. Residues were detected in fresh dung deposited by animals treated 10 days previously with a topical dose (500 microg/kg body weight) of ivermectin.
Floate
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KD
Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alb.
Taylor
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WG
Spooner
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RW
D000818:Animals; D000969:Antinematodal Agents / Q000032:analysis*; D002417:Cattle / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005243:Feces / Q000737:chemistry*; D007559:Ivermectin / Q000032:analysis*; D012680:Sensitivity and Specificity; D019155:Veterinary Drugs / Q000032:analysis*
D000969:Antinematodal Agents; D019155:Veterinary Drugs; D007559:Ivermectin