title: Block of the cell cycle of the yeast Saccharomyces cerevisiae by tyrphostin, an inhibitor of protein tyrosine kinase.
abstract: Tyrphostins are inhibitors of the epidermal growth factor receptor tyrosine kinase. To elucidate the biological function of protein tyrosine kinases in yeast cells, a mutant hypersensitive to tyrphostin was isolated and investigated for its response to the drug. The mutation was recessive and was designated tpt1 for tyrphostin hypersensitive. A tpt1 strain cannot grow in the presence of tyrphostin, implying that a biological process sensitive to tyrphostin is essential for cell growth. Microscopic observation indicated that large-budded cells were accumulated in the presence of the inhibitor. The results suggest the involvement of protein tyrosine phosphorylation in the cell cycle progression of Saccharomyces cerevisiae.
authors.affiliation: Central Research Laboratory, Nippon Hoechst Marion Roussel, Kawagoe, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fujimura
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects*; D003513:Cycloheximide / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D009154:Mutation; D009570:Nitriles / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology
chemical_list: D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D009570:Nitriles; D003513:Cycloheximide; D011505:Protein-Tyrosine Kinases