Amperometric lactate oxidase catheter for real-time lactate monitoring based on thin film technology.
An amperometric lactate oxidase catheter has been developed for in vivo application to real-time lactate monitoring. The electrochemical behaviour of the 1 x 3 mm Pt-Ag/AgCl thin film electrode is not significantly influenced by lactate oxidase-polyurethane covering. Gamma-irradiation (25 kGy) is suitable for the sterilization procedure. The final lactate catheter is characterized by a linear concentration range between 0.5 and 20 mmol/l lactate with a sensitivity around 2 nA mmol-1 l-1 lactate. The accuracy is demonstrated by the measurement of control sera. Both physiological and pathological materials correlate well with the declared values. The dry stored lactate catheter needs about 10 min for hydration and is characterized by response times t98% of less than 2 min. Ex vivo whole blood measurements using the lactate catheter (y) give a correlation with the BIOSEN Med L (x) of y = (1.010x + 0.513) mmol/l (r = 0.9748). Lactate values obtained by continuous catheter operation ex vivo correlate well with those obtained by BIOSEN Med L. First subcutaneous implantation (dog) underlines the characteristics obtained ex vivo: after 30 min hydration the lactate catheter follows the lactate concentration measured ex vivo with samples from the leg vein by BIOSEN Med L.
Pfeiffer
D
D
BST Bio Sensor Technologie GmbH, Berlin, Germany.
Möller
B
B
Klimes
N
N
Szeponik
J
J
Fischer
S
S
D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D004563:Electrochemistry; D004566:Electrodes; D007511:Ischemia / Q000097:blood / Q000175:diagnosis; D007773:Lactates / Q000097:blood*; D008297:Male; D006899:Mixed Function Oxygenases; D011140:Polyurethanes; D013242:Sterilization; D013499:Surface Properties; D013552:Swine
D007773:Lactates; D011140:Polyurethanes; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase