title: Olestra affects serum concentrations of alpha-tocopherol and carotenoids but not vitamin D or vitamin K status in free-living subjects.
abstract: Normal, healthy, free-living adults ingested either 18 g/d olestra, with or without 1.1 mg tocopheryl acetate/g olestra, or 18 g/d triglyceride placebo, for 16 wk in a double-blind, placebo-controlled study. Serum concentrations of alpha-tocopherol, alpha-carotene, beta-carotene, lycopene, lutein/zeaxanthin, retinol and cholesterol were measured biweekly. Serum 25-hydroxyvitamin D concentration, prothrombin time, partial thromboplastin time and plasma concentration of functional prothrombin (Simplastin-Ecarin assay) were measured at wk 0, 8 and 16. Relative to the placebo group, serum alpha-tocopherol concentration was reduced 6% for the group given 18 g/d olestra. Addition of tocopheryl acetate to olestra partially offset the effect of olestra. For the group given 18 g/d olestra plus 1.1 mg tocopheryl acetate/g olestra, serum alpha-tocopherol concentration was 4% less than the placebo value. Olestra reduced serum concentration of beta-carotene by 27%; the other carotenoids were similarly affected. Serum cholesterol concentration was reduced approximately 4.5% in the olestra groups, relative to placebo, but the differences were not significant. Serum triglycerides, serum 25-hydroxyvitamin D, prothrombin time, partial thromboplastin time or the plasma concentration of under-gamma-carboxylated prothrombin were unaffected by olestra. Clinical observations and laboratory measures indicated no health-related effects of olestra; mild-to-moderate transient gastrointestinal symptoms such as bloating, cramping, loose stools and diarrhea were reported by all groups.
authors.affiliation: The Procter & Gamble Company, Cincinnati, OH, 45224, USA.
authors.forename: B P
authors.initials: BP
authors.lastname: Koonsvitsky
authors.forename: D A
authors.initials: DA
authors.lastname: Berry
authors.forename: M B
authors.initials: MB
authors.lastname: Jones
authors.forename: P Y
authors.initials: PY
authors.lastname: Lin
authors.forename: D A
authors.initials: DA
authors.lastname: Cooper
authors.forename: D Y
authors.initials: DY
authors.lastname: Jones
authors.forename: J E
authors.initials: JE
authors.lastname: Jackson
mesh_terms: D000328:Adult; D002338:Carotenoids / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004311:Double-Blind Method; D019358:Fat Substitutes / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology*; D005260:Female; D006801:Humans; D014975:Lutein / Q000097:blood; D000077276:Lycopene; D008297:Male; D008875:Middle Aged; D009752:Nutritional Status / Q000187:drug effects*; D011517:Prothrombin Time; D013395:Sucrose / Q000031:analogs & derivatives* / Q000494:pharmacology; D014801:Vitamin A / Q000097:blood; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism*; D014810:Vitamin E / Q000097:blood*; D014812:Vitamin K / Q000378:metabolism*; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D004042:Dietary Fats, Unsaturated; D019358:Fat Substitutes; D005227:Fatty Acids; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D014801:Vitamin A; D014812:Vitamin K; D014807:Vitamin D; D014810:Vitamin E; D002338:Carotenoids; C041635:alpha-carotene; D013395:Sucrose; C013692:sucrose polyester; D002784:Cholesterol; C104450:25-hydroxyvitamin D; D000077276:Lycopene; D014975:Lutein