Iris pigment epithelial cells of long evans rats demonstrate phagocytic activity.
The phagocytic activities of iris pigment epithelial (IPE) cells and retinal pigment epithelial (RPE) cells of Long Evans rats towards latex beads and rod outer segments (ROS) were compared in vitro. IPE and RPE cells of Long Evans rats were isolated and pure cultures obtained. The cultures were incubated with latex beads, fixed, and analysed computer morphometrically, IPE and RPE cell cultures were also incubated with isolated ROS and examined using transmission electron microscopy. IPE cells were able to ingest latex beads. There was no significant difference between the number of latex particles phagocytized by IPE and RPE cells. After incubation with isolated ROS, IPE cells also recognized and ingested the ROS particles. However, the specific phagocytic capacity of IPE cells was 76% of that of RPE cells. The autologous IPE cells might have the potential to be used as an alternative to RPE cells for transplantation in the subretinal space.
Rezai
K A
KA
Department of Ophthalmology, University of Cologne, Joseph-Stelzmann-Strasse 9, Cologne, 50931, Germany.
Lappas
A
A
Farrokh-siar
L
L
Kohen
L
L
Wiedemann
P
P
Heimann
K
K
D000818:Animals; D002478:Cells, Cultured / Q000166:cytology; D007498:Iris / Q000166:cytology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010587:Phagocytosis; D010857:Pigment Epithelium of Eye / Q000166:cytology*; D051381:Rats; D012374:Rod Cell Outer Segment