Evidence for a role of C-terminal amino acid residues in skeletal muscle Ca2+ release channel (ryanodine receptor) function.
The effects of deleting 1, 3 and 15 amino acid residues from the highly conserved C-terminus of the tetrameric skeletal muscle ryanodine receptor (RyR) complex were determined. Immunoblot analysis indicated similar expression levels in HEK293 cells for full-length and mutant proteins. Full-length and RyR lacking the last amino acid showed [3H]ryanodine binding and single channel activities typical of native receptors. Deletion of 3 amino acids resulted in decreased activities, whereas deletion of 15 amino acids yielded an inactive RyR. These results suggest that the most 15 C-terminal amino acids are important for the expression of a functional RyR complex.
Gao
L
L
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260, USA.
Tripathy
A
A
Lu
X
X
Meissner
G
G
D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D004553:Electric Conductivity; D017353:Gene Deletion; D015870:Gene Expression; D015151:Immunoblotting; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism*; D016296:Mutagenesis; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics; D011817:Rabbits; D012433:Ryanodine / Q000378:metabolism; D019837:Ryanodine Receptor Calcium Release Channel; D013329:Structure-Activity Relationship
D015220:Calcium Channels; D009124:Muscle Proteins; D010446:Peptide Fragments; D019837:Ryanodine Receptor Calcium Release Channel; D012433:Ryanodine; D002118:Calcium