title: Vein quality influences neointimal hyperplasia in an organ culture model of human saphenous vein.
abstract: OBJECTIVE
  The severity of pre-existing pathological changes in human saphenous vein (HSV) correlates with the development of vein graft stenosis and graft patency. The aim of this study was to investigate the influence of pre-existing intimal hyperplasia on development of the neointima in vitro, using an organ culture model.
  
  
  METHODS
  Segments of HSV were harvested during coronary artery bypass surgery. Histology was performed on part of the vein; the remainder was maintained in culture. Pre-existing intimal thickness (PIT) in HSV from day 0 and neointimal thickness (NIT) in cultured HSV were measured using computerised image analysis on histological sections. Day 0 and 14 veins were compared with respect to intimal hyperplasia.
  
  
  RESULTS
  Twelve pairs of veins were examined. A mean tissue ATP of 297 nmoles/g wet weight for cultured. HSV confirmed cell viability. Mean PIT was 180 microns (CI: 86-274) and mean NIT was 60 microns (CI: 48-72), with a significant correlation between them using the Spearman Rank test (Rs = 0.72; p = 0.008).
  
  
  CONCLUSIONS
  Pre-existing vein quality as measured by PIT correlates with the development of neointimal hyperplasia in culture, adding further support to clinical evidence that poor vein quality predisposes to vein graft stenosis due to an inherent susceptibility to intimal hyperplasia.
authors.affiliation: Vascular Studies Unit, Bristol Heart Institute, U.K.
authors.forename: Y G
authors.initials: YG
authors.lastname: Wilson
authors.forename: A H
authors.initials: AH
authors.lastname: Davies
authors.forename: K
authors.initials: K
authors.lastname: Southgate
authors.forename: I C
authors.initials: IC
authors.lastname: Currie
authors.forename: E
authors.initials: E
authors.lastname: Sheffield
authors.forename: R N
authors.initials: RN
authors.lastname: Baird
authors.forename: P M
authors.initials: PM
authors.lastname: Lamont
authors.forename: G D
authors.initials: GD
authors.lastname: Angelini
mesh_terms: D006083:Graft Occlusion, Vascular / Q000209:etiology; D006801:Humans; D006965:Hyperplasia; D007091:Image Processing, Computer-Assisted; D009924:Organ Culture Techniques; D012501:Saphenous Vein / Q000473:pathology* / Q000637:transplantation; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D017539:Tunica Intima / Q000254:growth & development* / Q000473:pathology*; D014654:Vascular Patency