Polymerase chain reaction protocols for the detection of DNA from mucosal human papillomavirus types -6, -11, -16, -18, -31 and -33.
Individual types of human papillomaviruses (HPV) which infect mucosal surfaces have been implicated as the causative agents for carcinomas of the cervix, anus, penis, larynx and the buccal cavity, occasional periungal carcinomas, as well as benign anogenital warts. The identification of particular HPV types is thus important for: identifying patients with premalignant lesions who are at risk of progression to malignancy; epidemiological studies; studies of the natural history of these viruses; and even medico-legal cases of suspected sexual abuse of children. In this protocol we describe PCR assays for: the identification of DNA from the mucosal HPVs types -6, -11, -16, -18, -31 and -33; a consensus HPV PCR for detecting DNA from 20 characterised mucosal HPVs, as well as more than 25 novel HPVs; and, for a control PCR for beta-globin.
Mant
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Richard Dimbleby Laboratory of Cancer Virology, Department of Virology, Rayne Institute, United Medical and Dental School of Guys, St Thomas' Hospital, London, U.K.
Kell
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B
Best
J M
JM
Cason
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J
D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D009092:Mucous Membrane / Q000821:virology; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D014412:Tumor Virus Infections / Q000175:diagnosis*
D004279:DNA, Viral