Libraries from libraries: generation and comparison of screening profiles.
A positional scanning tetrapeptide library was chemically modified through alkylation and/or reduction of the amide bonds, thus generating three new combinatorial libraries with physico-chemical properties very different from the parent peptide library ('libraries from libraries'). Specific results were obtained with each of these libraries upon screening in kappa-opioid receptor binding and microdilution antimicrobial assays, illustrating the potential of the 'libraries from libraries' concept for the efficient generation of a variety of chemically diverse combinatorial libraries.
Houghten
R A
RA
Torrey Pines Institute for Molecular Studies, San Diego, CA 92121, USA.
Blondelle
S E
SE
Dooley
C T
CT
Dörner
B
B
Eichler
J
J
Ostresh
J M
JM
D000818:Animals; D001921:Brain / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004352:Drug Resistance, Microbial; D006168:Guinea Pigs; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology; D019151:Peptide Library; D017473:Receptors, Opioid, kappa / Q000187:drug effects; D018000:Receptors, Peptide / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
D009842:Oligopeptides; D019151:Peptide Library; D017473:Receptors, Opioid, kappa; D018000:Receptors, Peptide