Cloning and analysis of the cDNA encoding the rod G-protein transduction alpha, beta1 and gamma1 subunits from the canine retina.
The canine (Canis familiaris) retinal rod transducin (G(T)) alpha, beta1 and gamma1 subunits were sequenced. Cloning of the cDNAs was accomplished by polymerase chain reaction (PCR) using degenerate and wild type retinal cDNA libraries as templates. The deduced amino acid sequences were highly similar to rod transducins from other species: G(T alpha) differed by 5 amino acids from the corresponding human sequence, whereas beta1 and gamma1 were identical to human sequences. The coding sequence of rod transducin was evaluated as a possible cause for the recessively inherited retinal rod-cone degeneration: there were no nucleotide differences between the wild type and retinal degenerate strains.
Kylmä
T
T
Department of Clinical Sciences/Surgery, Faculty of Veterinary Medicine, University of Helsinki, Finland. tarja.kylma@helsinki.fi
Paulin
L
L
Hurwitz
M Y
MY
Hurwitz
R L
RL
Kommonen
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012162:Retinal Degeneration / Q000235:genetics / Q000662:veterinary*; D017948:Retinal Rod Photoreceptor Cells / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D015293:Transducin / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D015293:Transducin