title: Inclusion volume solid-phase synthesis.
abstract: Solid-phase synthesis of peptides was carried out using only the volume of the solvent included in the swollen solid-phase resin heads (inclusion volume synthesis). This approach enables (i) the use of higher concentrations of activated amino acids, resulting in increased coupling rates, (ii) drastically decreased consumption of solvents, and (iii) the construction of multiple peptide synthesizers having virtually no reaction vessels.
authors.affiliation: Torrey Pines Institute for Molecular Studies, San Diego, California 92121, USA.
authors.forename: J
authors.initials: J
authors.lastname: Eichler
authors.forename: R A
authors.initials: RA
authors.lastname: Houghten
authors.forename: M
authors.initials: M
authors.lastname: Lebl
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004126:Dimethylformamide; D005449:Fluorenes / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D010880:Piperidines; D011137:Polystyrenes / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000295:instrumentation* / Q000379:methods*; D014230:Triazoles / Q000737:chemistry
chemical_list: D000596:Amino Acids; D002234:Carbodiimides; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010455:Peptides; D010880:Piperidines; D011137:Polystyrenes; D014230:Triazoles; C101297:Tentagel resin; C032727:piperidine; C081611:1,3-diisopropylcarbodiimide; D004126:Dimethylformamide; C011852:1-hydroxybenzotriazole