Brefeldin A interferes with peroxisomal protein sorting in the yeast Hansenula polymorpha.
We have studied the effect of brefeldin A (BFA), a fungal toxin that interferes with coated vesicle formation, on the biogenesis of peroxisomes in the yeast Hansenula polymorpha. Addition of BFA (20 microg/ml) to cultures of H. polymorpha partially inhibited the development of peroxisomes and resulted in the reversible accumulation of newly synthesized peroxisomal membrane and matrix proteins at the endoplasmic reticulum. In contrast, BFA did not interfere with the selective degradation of peroxisomes. Taken together, our data suggest that the ER plays a crucial role in peroxisome biogenesis in H. polymorpha, possibly in the biosynthesis of the peroxisomal membrane.
Salomons
F A
FA
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Haren, The Netherlands.
van der Klei
I J
IJ
Kram
A M
AM
Harder
W
W
Veenhuis
M
M
D001692:Biological Transport; D020126:Brefeldin A; D003517:Cyclopentanes / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D008830:Microbodies / Q000187:drug effects* / Q000378:metabolism; D010843:Pichia / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*
D003517:Cyclopentanes; D011500:Protein Synthesis Inhibitors; D020126:Brefeldin A; D005947:Glucose