title: A pilot study identifying type V collagenolytic activity in human amniotic fluid.
abstract: Amniorrhexis complicates pregnancies if it occurs in a preterm pregnancy or remote from the onset of labor in a term pregnancy. There are different collagen types (I-V) that create the extracellular matrix of the amnion. Collagenases specific to these collagen types, with the exception of type V collagen, are found in human amniotic fluid, fibroblasts, polymorphonuclear leukocytes and bacteria. Type V collagen is a major component of the amniotic basement membrane and is responsible for maintaining a barrier to bacteria and to the loss of amniotic fluid. We sought to find evidence of type V collagenolytic activity in human amniotic fluid obtained from pregnancies in different clinical states.
authors.affiliation: Department of Obstetrics and Gynecology, Tripler Army Medical Center, Tripler AMC, Hawaii, USA.
authors.forename: W J
authors.initials: WJ
authors.lastname: Polzin
authors.forename: E G
authors.initials: EG
authors.lastname: Lockrow
authors.forename: W K
authors.initials: WK
authors.lastname: Morishige
mesh_terms: D000649:Amniocentesis; D000650:Amnion / Q000737:chemistry / Q000201:enzymology; D000653:Amniotic Fluid / Q000201:enzymology*; D001345:Autoradiography; D001419:Bacteria / Q000201:enzymology; D001485:Basement Membrane / Q000737:chemistry; D003094:Collagen / Q000032:analysis / Q000145:classification; D017364:Collagenases / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000201:enzymology; D005325:Fetal Organ Maturity; D005347:Fibroblasts / Q000201:enzymology; D005820:Genetic Testing; D006801:Humans; D008168:Lung / Q000196:embryology; D020784:Matrix Metalloproteinase 8; D003012:Microbial Collagenase / Q000032:analysis*; D009504:Neutrophils / Q000201:enzymology; D007752:Obstetric Labor, Premature / Q000201:enzymology; D010865:Pilot Projects; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents
chemical_list: D016326:Extracellular Matrix Proteins; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D003094:Collagen; D017364:Collagenases; C049755:type V collagen-degrading enzyme; D003012:Microbial Collagenase; D020784:Matrix Metalloproteinase 8