Suppression of tumor growth by the 3' untranslated region of mel-18 in 3Y1 cells transformed by the E6 and E7 genes of human papillomavirus type 18.
By introducing a cDNA library derived from rat embryonic fibroblast cells, we isolated several morphologically flat revertants of rat 3Y1 cells transformed by the E6 and E7 genes of human papillomavirus type 18 (HPV18). From one of the revertants, we recovered a 0.2-kb cDNA, N56, that suppresses the tumor growth of the transformed 3Y1 cells irrespective of the expression of the E6 and E7 genes. The nucleotide sequence of the cDNA was shown to be identical to that of the 3' untranslated region of a putative mammalian polycomb group gene, mel-18.
Ishiwatari
H
H
Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
Nakanishi
K
K
Kondoh
G
G
Hayasaka
N
N
Li
Q
Q
Yamashita
A
A
Inoue
H
H
Hakura
A
A
D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral / Q000187:drug effects*; D004268:DNA-Binding Proteins / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000472:pathogenicity*; D063150:Polycomb Repressive Complex 1; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D015678:Viral Structural Proteins / Q000235:genetics*
D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; C052633:E7 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; C489348:PCGF2 protein, human; D012097:Repressor Proteins; D015678:Viral Structural Proteins; D063150:Polycomb Repressive Complex 1