title: Calibration of micropipets using the bioluminescent protein aequorin.
abstract: A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.
authors.affiliation: Department of Chemical and Materials Engineering, University of Kentucky, Lexington 40506-0055, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Grosvenor
authors.forename: C L
authors.initials: CL
authors.lastname: Crofcheck
authors.forename: K W
authors.initials: KW
authors.lastname: Anderson
authors.forename: D L
authors.initials: DL
authors.lastname: Scott
authors.forename: S
authors.initials: S
authors.lastname: Daunert
mesh_terms: D000331:Aequorin; D002138:Calibration; D004864:Equipment and Supplies / Q000592:standards*; D008163:Luminescent Measurements; D008853:Microscopy; D015203:Reproducibility of Results
chemical_list: D000331:Aequorin