title: Effects of continuous distraction on cartilage in a moving joint: an investigation on adult rabbits.
abstract: An animal model, using distraction force on adult rabbits, was developed to study the effects of nonweight-bearing on articular cartilage in a moving joint. Histologic evaluation was used to compare the morphology of chondrocytes, safranin O intensity, cartilage thickness, and structural changes between the test and contralateral joints. At 3 and 6 weeks, the chondrocytes in superficial and intermediate zones were round, with an increase in cellular volume density and mean cell volume and with less metachromasia; the safranin O intensity and cartilage thickness were the same as in the controls. In cartilage of the 9-week group, mean cell volume decreased with cell cloning in the superficial zone, while numerical density increased and mean matrix volume per cell decreased in the superficial and intermediate zones. The cartilage, with a 34% reduction in thickness and a 53-72% decrease in safranin O intensity from the superficial to the deep zone, had superficial fibrotic proliferation, surface erosion or depression, and tidemark irregularity. Continuous distraction in a moving joint caused morphological changes in chondrocytes prior to degeneration of cartilage. These results support the hypothesis that the forces perceived by cells may dictate their shape and then stimulate alterations in cellular biochemistry and matrix metabolism.
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: S C
authors.initials: SC
authors.lastname: Hung
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: R
authors.initials: R
authors.lastname: Shiro
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Kawahara
authors.forename: T
authors.initials: T
authors.lastname: Kurokawa
mesh_terms: D000367:Age Factors; D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology / Q000502:physiology* / Q000601:surgery; D004396:Coloring Agents; D005109:Extracellular Matrix / Q000473:pathology; D007719:Knee Joint / Q000473:pathology / Q000502:physiology* / Q000601:surgery; D008297:Male; D010619:Phenazines; D011817:Rabbits; D013194:Staining and Labeling; D016474:Weight-Bearing / Q000502:physiology
chemical_list: D004396:Coloring Agents; D010619:Phenazines; C009195:safranine T