Antisense expression of the peptide transport gene AtPTR2-B delays flowering and arrests seed development in transgenic Arabidopsis plants.
Previously, we identified a peptide transport gene, AtPTR2-B, from Arabidopsis thaliana that was constitutively expressed in all plant organs, suggesting an important physiological role in plant growth and development. To evaluate the function of this transporter, transgenic Arabidopsis plants were constructed expressing antisense or sense AtPTR2-B. Genomic Southern analysis indicated that four independent antisense and three independent sense AtPTR2-B transgenic lines were obtained, which was confirmed by analysis of the segregation of the kanamycin resistance gene carried on the T-DNA. RNA blot data showed that the endogenous AtPTR2-B mRNA levels were significantly reduced in transgenic leaves and flowers, but not in transgenic roots. Consistent with this reduction in endogenous AtPTR2-B mRNA levels, all four antisense lines and one sense line exhibited significant phenotypic changes, including late flowering and arrested seed development. These phenotypic changes could be explained by a defect in nitrogen nutrition due to the reduced peptide transport activity conferred by AtPTR2-B. These results suggest that AtPTR2-B may play a general role in plant nutrition. The AtPTR2-B gene was mapped to chromosome 2, which is closely linked to the restriction fragment length polymorphism marker m246.
Song
W
W
Centre for Legume Research, University of Tennessee, Knoxville 37996-0845, USA.
Koh
S
S
Czako
M
M
Marton
L
L
Drenkard
E
E
Becker
J M
JM
Stacey
G
G
D016960:Agrobacterium tumefaciens; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002874:Chromosome Mapping; D016373:DNA, Antisense; D004269:DNA, Bacterial / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D026901:Membrane Transport Proteins; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000096:biosynthesis; D012639:Seeds / Q000502:physiology; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D016373:DNA, Antisense; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D026901:Membrane Transport Proteins; C098241:PTR2 protein, Arabidopsis; D010940:Plant Proteins; D012333:RNA, Messenger; C036483:T-DNA