title: Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells.
abstract: Effects of intraluminal pressure on cultured intestinal epithelial cells were assessed by measuring cell proliferation and DNA synthesis after exposure to various pressures. Pressures of 40 to 120 mm Hg promoted cell proliferation and DNA synthesis. Pressure-induced DNA synthesis was further enhanced by the addition of interleukin-2, suggesting the regulation of intestinal epithelial growth by pressure in coordination with cytokines. Pretreatment with either a phospholipase C inhibitor or protein kinase C inhibitor significantly inhibited DNA synthesis promoted by pressure and interleukin-2. This study demonstrates a novel mechanism whereby pressure regulates cell growth in intestinal epithelial cells, possibly via activation of phospholipase C and protein kinase C.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hirokawa
authors.forename: S
authors.initials: S
authors.lastname: Miura
authors.forename: T
authors.initials: T
authors.lastname: Shigematsu
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: R
authors.initials: R
authors.lastname: Hokari
authors.forename: H
authors.initials: H
authors.lastname: Higuchi
authors.forename: I
authors.initials: I
authors.lastname: Kurose
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Nakaki
authors.forename: H
authors.initials: H
authors.lastname: Ishii
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002219:Carbamates / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D004261:DNA Replication; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D048448:Phenylcarbamates; D010812:Physical Stimulation; D011312:Pressure; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors
chemical_list: D002147:Calmodulin; D002219:Carbamates; D004791:Enzyme Inhibitors; D007376:Interleukin-2; D048448:Phenylcarbamates; D013449:Sulfonamides; C025508:2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate; C017967:W 7; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D010738:Type C Phospholipases