Remodeling of regulatory nucleoprotein complexes on the Xenopus hsp70 promoter during meiotic maturation of the Xenopus oocyte.
Transcriptional repression occurs during meiotic maturation of Xenopus oocytes. Injection of a DNA template containing an hsp70 promoter into Xenopus oocytes, followed by progesterone-induced maturation has been used to demonstrate a dynamic competition between the assembly of transcription factor-containing nucleoprotein complexes and repressive nucleosomal arrays during the maturation process. In particular, it is shown that increased levels of injected heat shock protein, the transcriptional activator Gal4-VP16 or the DNA template itself all lead to reduced repression of transcription on maturation. Conversely, injection of additional histone increases repression. Repression of transcription is shown to be accompanied by the formation of a more regular array of nucleosomes and by an increase in the efficiency of nucleosome assembly on the injected plasmid. Meiotic maturation is therefore accompanied by replacement of transcription factor complexes by a repressive chromatin environment.
Landsberger
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N
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892-5431, USA.
Wolffe
A P
AP
D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D018983:DNA Footprinting; D003850:Deoxyribonuclease I / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005656:Fungal Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D008540:Meiosis / Q000235:genetics*; D008845:Microinjections; D009698:Nucleoproteins / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014981:Xenopus
D002843:Chromatin; D005656:Fungal Proteins; C070633:Gal-VP16; D018840:HSP70 Heat-Shock Proteins; D006657:Histones; D009698:Nucleoproteins; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors; D011374:Progesterone; D003850:Deoxyribonuclease I