Characterization of fluoranthene- and pyrene-degrading Mycobacterium-like strains by RAPD and SSU sequencing.
Bacterial isolates were obtained from two sites in New Zealand contaminated with polycyclic aromatic hydrocarbons. Isolates capable of degrading polycyclic aromatic hydrocarbons were characterized in two mycobacterial groups according to phenotypic properties. These groups were supported by random amplified polymorphic DNA analysis. Nucleotide sequences of 16S ribosomal RNA genes from isolates representing each group were determined and compared with other mycobacterial 16S ribosomal RNA sequences. The taxonomic relationships of these isolates are considered.
Lloyd-Jones
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Manaaki Whenua, Landcare Research, Hamilton, New Zealand. lloyd-jonesg@landcare.cri.nz
Hunter
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DW
D001673:Biodegradation, Environmental; D005449:Fluorenes / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics* / Q000378:metabolism*; D009520:New Zealand; D010802:Phylogeny; D011721:Pyrenes / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000378:metabolism
D005449:Fluorenes; D011721:Pyrenes; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C007738:fluoranthene; C030984:pyrene