Metabolism of dolichol, dolichoic acid and nordolichoic acid in cultured cells.
The uptake and metabolism of [1-(14)C]-labelled dolichol, dolichoic acid and nordolichoic acid were investigated in MDCK and HepG2 cells. Each of the three isoprenoids, bound to human serum albumin, was taken up effectively. None of the compounds was broken down in HepG2 cells, although these converted dolichol into fatty acid esters. In MDCK cells dolichoic acid gave rise to the formation of [14C]CO2 and radiolabelled formic acid, indicating that dolichoic acid can be broken down by alpha-oxidation. Dolichoic acid was also converted to a mixture of polar compounds, possibly polyols. MDCK cells generated radiolabelled CO2 from nordolichoic acid, presumably through beta-oxidation, although we could not find any labelled propionic acid. No oxidative breakdown of dolichol was found, apparently due to the lack of or very low conversion to dolichoic acid.
Van Houte
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HA
Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Belgium.
Van Veldhoven
P P
PP
Mannaerts
G P
GP
Baes
M I
MI
Declercq
P E
PE
D000818:Animals; D004285:Dogs; D004286:Dolichols / Q000378:metabolism*; D006801:Humans; D013729:Terpenes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D004286:Dolichols; D013729:Terpenes; C107327:dolichoic acid; C107328:nordolichoic acid