Conserved Y-chromosomal location of TSPY in Bovidae.
We determined the chromosomal location of TSPY, the testis-specific protein, Y-encoded, by fluorescence in situ hybridization (FISH) to chromosome spreads of cattle, goat and sheep. Using a cloned polymerase chain reaction (PCR) product of one bovine TSPY family member, we were able to show a conserved Y chromosomal localization for TSPY in all three species. In contrast to a limited regional distribution of TSPY FISH signals on the chromosome of man, other primates, great apes, goat and sheep, in cattle TSPY-related sequences appear to be spread over most of the Y chromosome. The painting effect observed in this species reflects the higher complexity of the bovine TSPY gene family, being composed not only of a tandemly repeated cluster, but harbouring a large number of different family members dispersed all over the Y chromosome.
Vogel
T
T
Institut für Humangenetik, Medizinische Hochschule Hannover, Germany.
Borgmann
S
S
Dechend
F
F
Hecht
W
W
Schmidtke
J
J
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics*; D006041:Goats / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D012756:Sheep / Q000235:genetics*; D014157:Transcription Factors; D014998:Y Chromosome / Q000235:genetics*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors