Identification of interleukin-6 producing fibroblastoid cells in cerebrospinal fluid from patients with leukemic meningitis.
Cytokine producing native cells in cerebrospinal fluid (CSF) have not been identified. So, we investigated the cytokine producing ability of floating cells in CSF from patients with leukemic meningitis. Morphologic study revealed that established cell lines were polygonal or elongated in shape and had an abundant and irregular branched cytoplasm. Immunocytochemical analysis demonstrated positive reactivity with monoclonal anti-fibroblast antibody only. Interleukin-6 (IL-6) was constitutively produced in vitro by these cell lines; both interleukin-1 and lipopolysaccharides significantly increased its synthesis. These findings imply that these fibroblastoid cells are floating in CSF of patients with leukemic meningitis and produce IL-6 in response to various inflammatory stimulations in vivo.
Uozumi
K
K
Second Department of Internal Medicine, Faculty of Medicine, Kagashima University, Japan.
Ohno
N
N
Nakahara
K
K
Makino
T
T
Utsunomiya
A
A
Hanada
S
S
Arima
T
T
D000328:Adult; D000368:Aged; D002460:Cell Line; D002555:Cerebrospinal Fluid / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000096:biosynthesis*; D015458:Leukemia, T-Cell / Q000134:cerebrospinal fluid* / Q000276:immunology* / Q000473:pathology; D008297:Male; D008581:Meningitis / Q000134:cerebrospinal fluid* / Q000276:immunology* / Q000473:pathology; D008875:Middle Aged
D015850:Interleukin-6