title: Integrins beta 5, beta 3 and alpha v are apically distributed in endometrial epithelium.
abstract: Several adhesion molecules have been shown to occur at the surface of endometrial cells. One of these is the integrin alpha v subunit which associates with various beta chains including beta 5. We demonstrate the presence of integrin beta 5 polypeptide in human endometrial epithelial cells throughout the menstrual cycle using immunocytochemistry with monospecific antibodies, and at the mRNA level by thermal amplification from endometrial cDNA. Integrin beta 5 is also found in a population of bone marrow-derived cells. A notable feature of the distribution of the beta 5 subunit in the glandular and luminal epithelium is its apical localization, which may suggest an involvement in implantation. However, no evidence was found for regulated expression of epithelial beta 5. In mouse, the beta 5 subunit is found at both the apical and basal surface of epithelial cells and expression is essentially oestrous cycle-independent. Comparisons are made in both species with the distribution of the alpha v and beta 3 subunits which also localize to the apical epithelium.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Manchester, UK.
authors.forename: J D
authors.initials: JD
authors.lastname: Aplin
authors.forename: C
authors.initials: C
authors.lastname: Spanswick
authors.forename: F
authors.initials: F
authors.lastname: Behzad
authors.forename: S J
authors.initials: SJ
authors.lastname: Kimber
authors.forename: L
authors.initials: L
authors.lastname: Vićovac
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D010064:Embryo Implantation; D004717:Endometrium / Q000737:chemistry* / Q000166:cytology; D004848:Epithelium / Q000737:chemistry; D004971:Estrus; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D066298:In Vitro Techniques; D039564:Integrin alphaV; D039641:Integrin beta Chains; D039661:Integrin beta3; D016023:Integrins / Q000032:analysis*; D051379:Mice; D010052:Ovariectomy; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039564:Integrin alphaV; D039641:Integrin beta Chains; D039661:Integrin beta3; D016023:Integrins; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; C084344:integrin beta5