Detection of interleukin-1 beta in sera and colostrum of dairy cattle and in sera of neonates.
In order to obtain basic information about bovine interleukin-1 (IL-1 beta), levels of IL-1 beta in sera and milk of clinically normal mature Holstein cattle before and after parturition and in sera of newborn calves were examined by ELISA. The level of IL-1 beta was undetectable in sera of mature cattle around the time of artificial insemination, but the concentration gradually increased and reached a peak at parturition and then decreased again to an undetectable level. IL-1 beta in milk was detected on the day of parturition but not thereafter. IL-1 beta mRNA was detected by reverse transcription-polymerase chain reaction in the cells from milk collected during 20 days before and 2 to 3 days after parturition, but was not detected thereafter. Although IL-1 beta was not detected in all the sera of newborn calves, the concentration transiently increased with peak titers on day 3 and became undetectable by day 14 after birth. Newborns that showed serum IL-1 beta on day 3 had been fed on colostrum in which the IL-1 beta concentration was significantly higher than that in colostrum that had been fed to newborns having no detectable IL-1 beta on day 3. These results indicate that IL-1 beta is induced in association with pregnancy in healthy dairy cattle and that the cytokine might be transferred to neonates via colostrum.
Goto
M
M
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Maruyama
M
M
Kitadate
K
K
Kirisawa
R
R
Obata
Y
Y
Koiwa
M
M
Iwai
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn / Q000097:blood; D002417:Cattle; D003126:Colostrum / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007375:Interleukin-1 / Q000032:analysis* / Q000097:blood; D007774:Lactation; D008297:Male; D008431:Maternal-Fetal Exchange / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D013997:Time Factors
D007375:Interleukin-1