title: Antioxidant status of elderly chronic renal patients treated by continuous ambulatory peritoneal dialysis.
abstract: BACKGROUND
  An oxidative stress has been reported in patients with chronic renal failure (CRF) treated by haemodialysis. To our knowledge, only scant information is available concerning CRF patients treated by continuous ambulatory peritoneal dialysis (CAPD) with regard to their redox and nutritional status.
  
  
  METHODS
  The oxidative stress and the biological nutritional status were evaluated in 20 elderly CRF patients treated by CAPD, compared with a control group of 30 elderly non-CRF patients. Plasma peroxidation products were assayed as thiobarbituric acid-reactive substances (TBARS), and two enzymatic antioxidant systems were determined: erythrocyte superoxide dismutase (SOD), glutathione peroxidase activity in plasma (P-GSH-Px) and in erythrocytes (E-GSH-Px). Selenium, vitamin E, beta-carotene and vitamin A were evaluated as plasma non-enzymatic antioxidants. Nutritional status and iron status were assessed by determining serum albumin, prealbumin, iron, ferritin and transferrin concentrations.
  
  
  RESULTS
  Plasma TBARS concentration was high in both groups (CAPD: 1.37 +/- 0.06 mumol/l versus non-CRF: 1.41 +/- 0.06 mumol/l; P = NS), compared with usual values (0.60 to 1.20 mumol/l), on account of the patients' ages. SOD and E-GSH-Px activities were normal in both groups. A significant lowering in P-GSH-Px activity was observed only in CAPD patients (211 +/- 14 U/l, usual values: 480 to 650 U/l). Plasma selenium concentration, decreased in both groups, was significantly lower in CAPD than in non-CRF patients (P < 0.01). Plasma vitamin E, beta-carotene and vitamin A concentrations were significantly enhanced only in CAPD patients (P < 0.0001, P < 0.005 and P < 0.0001, respectively. Biological nutritional markers were similar in both groups and within usual values.
  
  
  CONCLUSIONS
  This study demonstrated the existence of an oxidative stress in CAPD-treated elderly CRF patients, evidenced by a decrease in plasma selenium levels and in P-GSH-Px activity. However, plasma TBARS were not higher in CAPD patients than in age-matched non-CRF control subjects, probably on account on the patients' ages.
authors.affiliation: Laboratoire de Biochimie, Groupe Hospitalier Pitie-Salpêtrière, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Bonnefont-Rousselot
authors.forename: M C
authors.initials: MC
authors.lastname: Jaudon
authors.forename: B
authors.initials: B
authors.lastname: Issad
authors.forename: P
authors.initials: P
authors.lastname: Cacoub
authors.forename: F
authors.initials: F
authors.lastname: Congy
authors.forename: C
authors.initials: C
authors.lastname: Jardel
authors.forename: J
authors.initials: J
authors.lastname: Delattre
authors.forename: C
authors.initials: C
authors.lastname: Jacobs
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000032:analysis*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000628:therapy; D008297:Male; D010531:Peritoneal Dialysis, Continuous Ambulatory; D012177:Retinol-Binding Proteins / Q000032:analysis; D054839:Retinol-Binding Proteins, Plasma; D014810:Vitamin E / Q000032:analysis; D019207:beta Carotene / Q000097:blood
chemical_list: D000975:Antioxidants; D012177:Retinol-Binding Proteins; D054839:Retinol-Binding Proteins, Plasma; D019207:beta Carotene; D014810:Vitamin E; D005979:Glutathione Peroxidase