Semi-quantitative assay of D antigen site density by flow cytometric analysis.
This study describes the comparative measurement of D antigen site density using a fluorescent indirect antiglobulin test (FIAT) read by flow cytometry. The test results confirmed the continuum of D antigen strength from weakest D through to R2R2 and also allowed the majority of weak D samples to be adequately distinguished from D-negative samples.
Nicholson
G
G
West Midlands Regional Blood Transfusion Service, Edgbaston, Birmingham, U.K.
Lawrence
A
A
Ala
F A
FA
Bird
G W
GW
D004912:Erythrocytes / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D010455:Peptides / Q000276:immunology; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D007140:Immunoglobulin Fab Fragments; D010455:Peptides; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen