In vitro selection of a 7-methyl-guanosine binding RNA that inhibits translation of capped mRNA molecules.
Using systematic evolution of ligands by exponential enrichment (SELEX), an RNA molecule was isolated that displays a 1,000-fold higher affinity for guanosine residues that carry an N-7 methyl group than for nonmethylated guanosine residues. The methylated guanosine residue closely resembles the 5' terminal cap structure present on all eukaryotic mRNA molecules. The cap-binding RNA specifically inhibited the translation of capped but not uncapped mRNA molecules in cell-free lysates prepared from either human HeLa cells or from Saccharomyces cerevisiae. These findings indicate that the cap-binding RNA will also be useful in studies of other cap-dependent processes such as pre-mRNA splicing and nucleocytoplasmic mRNA transport.
Haller
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Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5402, USA.
Sarnow
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P
D001483:Base Sequence; D006151:Guanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012331:RNA, Fungal / Q000032:analysis* / Q000378:metabolism; D012333:RNA, Messenger / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae
D012331:RNA, Fungal; D012333:RNA, Messenger; D006151:Guanosine; C016578:7-methylguanosine; D012313:RNA