In vitro toxicity and formation of early conjugates in Caco-2 cell line treated with clenbuterol, salbutamol and isoxsuprine.
Caco-2, human intestinal cell line able to differentiate in long-term culture, has been used to assess the cytotoxicity of the beta-agonists clenbuterol, salbutamol and isoxsuprine, also used at high doses to obtain lean meat in food producing animals, and to investigate the eventual in vitro formation of early conjugates of these compounds. For this purpose, the cells have been characterized for the activity of UDP-glucuronyltransferase, which is present and increases in the differentiated cells, and for the beta-receptors' binding characteristics, which are those of beta 1 and beta 2 subtypes. Isoxsuprine was shown to be the most toxic, followed by clenbuterol and salbutamol. Conjugates have been observed after incubation of the cells both with the lowest isoxsuprine and the highest salbutamol concentrations. No conjugates were detected in the case of clenbuterol.
Stammati
A
A
Laboratorio di Tossicologia Comparata ed Ecotossicologia, Istituto Superiore di Sanità, Roma, Italy.
Badino
P
P
De Angelis
I
I
Re
G
G
Vincentini
O
O
Zanetti
C
C
Zucco
F
F
Brambilla
G
G
D000318:Adrenergic beta-Agonists / Q000378:metabolism* / Q000633:toxicity*; D000420:Albuterol / Q000378:metabolism / Q000633:toxicity; D018938:Caco-2 Cells / Q000187:drug effects* / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002976:Clenbuterol / Q000378:metabolism / Q000633:toxicity; D014453:Glucuronosyltransferase / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007410:Intestinal Diseases / Q000139:chemically induced; D007556:Isoxsuprine / Q000378:metabolism / Q000633:toxicity
D000318:Adrenergic beta-Agonists; D014453:Glucuronosyltransferase; D000420:Albuterol; D007556:Isoxsuprine; D002976:Clenbuterol