Sequence and genetic analysis of the hemin storage (hms) system of Yersinia pestis.
We have sequenced a region from the pigmentation (pgm) locus of Yersinia pestis KIM6+ that is identified with the exogenous hemin storage (Hms+) phenotype in cells grown at 26 but not at 37 degrees C. The hmsHFRS locus encodes a putative polycistronic operon, with hmsH encoding an outer membrane protein with a deduced molecular mass of 93.4/89.5 (unprocessed/processed) kDa. The mature HmsH 788 aa polypeptide has a pI of 4.99. The hmsF gene has an open reading frame of 654 aa, encoding a 74.6/72.2 kDa OM protein with a pI of 5.16 when processed. A deduced 423 aa, 52 kDa protein with a pI of 10.83 is encoded by hmsR. HmsR has a basic, hydrophilic, and alpha-helical carboxyl terminus; 13 aa at the amino-terminal end and a 'KRKRAR' sequence at the carboxy-terminal end are essential for an Hms+ phenotype. The hmsS gene encodes a hypothetical 155 aa, 17.5 kDa protein with a pI of 6.68. Hms- Y. pestis strain M23-2 transformed with the cloned hmsHFRS locus developed an Hms(c) phenotype (Hms+ at 26-37 degrees C) due to mutations in genes outside the pgm locus.
Lillard
J W
JW
Department of Microbiology and Immunology, University of Kentucky, Lexington 40536-0084, USA.
Fetherston
J D
JD
Pedersen
L
L
Pendrak
M L
ML
Perry
R D
RD
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D006427:Hemin / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D010858:Pigmentation / Q000235:genetics; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D015010:Yersinia pestis / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C082669:HmsF protein, Yersinia pestis; C082666:HmsH protein, Yersinia pestis; C082670:HmsR protein, Yersinia pestis; D008565:Membrane Proteins; D006427:Hemin