Genetic and developmental control of nuclear accumulation of COP1, a repressor of photomorphogenesis in Arabidopsis.
Using a beta-glucuronidase (GUS) reporter-COP1 fusion transgene, it was shown previously that Arabidopsis COP1 acts within the nucleus as a repressor of seedling photomorphogenic development and that high inactivation of COP1 was accompanied by a reduction of COP1 nuclear abundance (A.G. von Arnim, X.-W. Deng [1994] Cell 79: 1035-1045). Here we report that the GUS-COP1 fusion transgene can completely rescue the defect of cop1 mutations and thus is fully functional during seedling development. The kinetics of GUS-COP1 relocalization in a cop1 null mutant background during dark/light transitions imply that the regulation of the functional nuclear COP1 level plays a role in stably maintaining a committed seedling's developmental fate rather than in causing such a commitment. Analysis of GUS-COP1 cellular localization in mutant hypocotyls of all pleiotropic COP/DET/FUS loci revealed that nuclear localization of GUS-COP1 was diminished under both dark and light conditions in all mutants tested, whereas nuclear localization was not affected in the less pleiotropic cop4 mutant. Using both the brassinosteroid-deficient mutant det2 and brassinosteroid treatment of wild-type seedlings, we have demonstrated that brassinosteroid does not control the hypocotyl cell elongation through regulation nuclear localization of COP1. The growth regulator cytokinin, which also dramatically reduced hypocotyl cell elongation in the absence of light, did not prevent GUS-COP1 nuclear localization in dark-grown seedlings. Our results suggest that all of the previously characterized pleiotropic COP/DET/FUS loci are required for the proper nuclear localization of the COP1 protein in the dark, whereas the less pleiotropic COP/DET loci or plant regulators tested are likely to act either downstream of COP1 or by independent pathways.
von Arnim
A G
AG
Department of Biology, Yale University, New Haven, Connecticut 06520-8104, USA.
Osterlund
M T
MT
Kwok
S F
SF
Deng
X W
XW
D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism*; D003433:Crosses, Genetic; D003624:Darkness; D018507:Gene Expression Regulation, Developmental; D005966:Glucuronidase / Q000096:biosynthesis; D018546:Hypocotyl; D008027:Light; D009024:Morphogenesis / Q000502:physiology / Q000528:radiation effects; D016296:Mutagenesis; D010641:Phenotype; D010937:Plant Growth Regulators / Q000502:physiology; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D044767:Ubiquitin-Protein Ligases
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C471983:AT2G32950 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases; D005966:Glucuronidase