[Preliminary evaluation of a method for detection of individual malignant melanoma cells in peripheral blood while monitoring the course of the malignancy and its treatment].
Using reverse transcription and polymerase chain reaction (PCR) with primers specific for tyrosinase the individual melanoma cells were detected in peripheral blood of patients in different stages of disease, after excision of primary lesion and prior and after chemotherapy. No relation between stage of disease (including situations with overt generalized spread of melanoma) and probability of positive PCR reaction detecting transcript for tyrosinase gene was found. Many patients in III and IV stages were negative for prolonged periods. Therefore, this method cannot be used for monitoring of all patients, because many of them are negative prior as well as after chemotherapy. With regard to the effects of therapy, the patients differed one to another and although some persons positive prior treatment became negative thereafter, a similar number of initially negative patients became positive after treatment.
Szenajch
J
J
Zakład Immunologii, Centralny Szpital Kliniczny Wojskowej, Akademii Medycznej w Warszawie.
Kozak
A
A
Kruszewski
A A
AA
Chomicka
M
M
Struzyna
J
J
Jedrzejczak
W W
WW
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000097:blood* / Q000517:prevention & control; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000097:blood* / Q000473:pathology
Wstepna ocena metody wykrywania pojedynczych komórek czerniaka złosśliwego we krwi obwodowej w monitorowaniu przebiegu choroby nowotworowej i jej leczenia.