Structure and properties of TentaGel resin beads: implications for combinatorial library chemistry.
In view of the widespread use of TentaGel resin beads for the synthesis of combinatorial libraries, the properties of TentaGel resin have been examined using a combination of confocal laser microscopy and NMR spectroscopy. Evidence is presented that trypsin, a 23.5-kDa enzyme, can penetrate to the core of 90-microns TentaGel beads, and that the matrix of such beads permits molecular motion at a similar rate to that in solution. The beads act as a separate gel phase rather than as a porous solid. These conclusions have important implications for the bioassay of on-bead combinatorial chemical libraries.
Quarrell
R
R
Dyson Perrins Laboratory, Oxford University, U.K.
Claridge
T D
TD
Weaver
G W
GW
Lowe
G
G
D003903:Deuterium; D019020:Directed Molecular Evolution / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D009682:Magnetic Resonance Spectroscopy; D018613:Microscopy, Confocal; D015394:Molecular Structure; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012996:Solutions; D012997:Solvents; D014357:Trypsin
D011137:Polystyrenes; D012117:Resins, Synthetic; D012996:Solutions; D012997:Solvents; C101297:Tentagel resin; D003903:Deuterium; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate