Multianalyte biosensors on optical imaging bundles.
We present an optical biosensor design that expands the utility of enzyme biosensors. These biosensors are fabricated by site-selective photodeposition of analyte-sensitive polymer matrices on optical imaging fibres. These dual-analyte arrays allow for the simultaneous, independent measurement of the analyte of interest and the transducing analyte. The first integrated optical-biosensors using this design have been prepared that allow both the dependent and independent analytes to be measured simultaneously, for example penicillin and pH (Healey & Walt, 1995) or glucose and O2 (Li & Walt, 1995). Independent measurement of the transducing analyte allows penicillin or glucose to be quantitated in the presence of a concurrent pH or O2 change, respectively. Penicillin can be measured in the range 0.25-10.0 mM in the pH range 6.2-7.5. Glucose can be measured in the range 0.6-20.0 mM in the O2 range 20-100%. The utility of the sensor design was demonstrated by using the penicillin-dual-analyte biosensor to quantitate penicillin produced during a Penicillium chrysogenum fermentation.
Healey
B G
BG
Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Li
L
L
Walt
D R
DR
D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D005285:Fermentation; D005336:Fiber Optic Technology; D005947:Glucose / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen; D010408:Penicillium chrysogenum; D015398:Signal Transduction
D004800:Enzymes, Immobilized; D005947:Glucose; D010100:Oxygen