Identification of a strong promoter of bacteriophage MB78 that lacks consensus sequence around minus 35 region and interacts with phage specific factor.
A strong promoter of bacteriophage MB78 does not have minus 35 consensus sequence although it has a TGn motif immediately upstream of minus 10 sequence as well as the AT rich UP element. It is efficiently recognised by the sigma 70 RNA polymerase, however, a phage-specific factor competes with sigma 70 RNA polymerase for binding to this region, the binding of the factor being stronger than that of the polymerase. Contrary to the reports in the literature the polymerase appears not to bind to the UP element whereas the phage-specific factor does. The latter seems to be involved in the regulation of the promoter activity.
Zargar
M A
MA
Molecular Biology Unit, Banaras Hindu University, Varanasi, U.P., India.
Pandey
B
B
Sharma
R
R
Chakravorty
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D001667:Binding, Competitive; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012483:Salmonella Phages / Q000235:genetics* / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism / Q000821:virology; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor / Q000378:metabolism
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D015246:Deoxyribonuclease EcoRI