A plasmid-mediated CMY-2 beta-lactamase from an Algerian clinical isolate of Salmonella senftenberg.
Multiresistance to antibiotics including beta-lactams, e.g. cefoxitin, was transferred by conjugation to Escherichia coli strain C1a from a clinical isolate of Salmonella senftenberg recovered from stools of an Algerian child. The susceptibility pattern to beta-lactams was similar to the profile mediated by an AmpC-type beta-lactamase. By biochemical analysis, typical AmpC-type enzyme substrate and inhibition profiles were obtained. Finally, an ampC plasmid-encoded beta-lactamase gene was cloned and sequenced. Its deduced amino acid sequence confirmed its identity as a class C beta-lactamase. It showed 99.5% sequence identity with the plasmid-mediated beta-lactamase CMY-2. The differences in the amino acid sequences of the two enzymes were located in the signal peptide.
Koeck
J L
JL
Laboratoire de Biologie Médicale, Hôpital d'instruction des armées Bégin, Saint-Mandé, France. jlkoeck@filnet.fr
Arlet
G
G
Philippon
A
A
Basmaciogullari
S
S
Thien
H V
HV
Buisson
Y
Y
Cavallo
J D
JD
D000462:Algeria; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012475:Salmonella / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases