title: Influenza A virus affects the response of human peripheral blood mononuclear cells to phytohaemagglutinin A by altering the cytoskeleton.
abstract: In the present study, we demonstrate that the infection of human peripheral blood mononuclear cells (PBMC) with influenza A virus caused changes in intracellular fluorescein fluorescence polarization (IFFP) which, as previously described, reflect alterations in the polymerization of the cytoskeleton. Kinetic measurements revealed two cycles of an approximate 10% decrease in IFFP within 3.5 and 5 h after infection. Infection win influenza A virus also altered the response of PBMC to phytohaemagglutinin (PHA), which was manifested as changes of 5.3 and 4% in IFFP at 1 and 2 h after infection, respectively. the changes in IFFP correlated with DNA synthesis measured 72 h after exposure to PHA. These results show the ability of IFFP measurements to identify early intracellular metabolic events induced in virus-infected cells.
authors.affiliation: Tel-Aviv Sourasky Medical Center, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Eisenthal
authors.forename: O
authors.initials: O
authors.lastname: Marder
authors.forename: B
authors.initials: B
authors.lastname: Lifschitz-Mercer
authors.forename: Y
authors.initials: Y
authors.lastname: Skornick
authors.forename: D
authors.initials: D
authors.lastname: Fixler
authors.forename: R
authors.initials: R
authors.lastname: Avtalyon
authors.forename: R
authors.initials: R
authors.lastname: Tirosh
authors.forename: M
authors.initials: M
authors.lastname: Deutsch
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000473:pathology* / Q000821:virology*; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D008213:Lymphocyte Activation / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology*
chemical_list: D010835:Phytohemagglutinins