title: Bispecific rabbit Fab'-bovine serum albumin conjugate used in hemagglutination immunoassay for beta-microseminoprotein.
abstract: A polyclonal bispecific (bifunctional) antibody was prepared to develop a hemagglutination immunoassay for beta-microseminoprotein (beta-MSP), a predominant seminal protein. Three types of F(ab')2 fragments of rabbit IgG, affinity-purified anti-human red blood cell (RBC) F(ab')2 nonaffinity-purified anti-beta-MSP F(ab')2 and nonspecific (nonimmunized) F(ab')2, were mixed to obtain a F(ab')2 mixture containing 10% anti-RBC molecules and 10% anti-beta-MSP molecules. Fab' was obtained from the F(ab')2 mixture, and then reacted with maleimide-activated bovine serum albumin (BSA) at a molar ratio of 10:1. As estimated by the decrease in the maleimide content, approximately 7 Fab' molecules were introduced per one BSA molecule. The bispecific (anti-beta-MSP and anti-RBC) Fab'-BSA conjugate thus prepared was incubated successively with a human RBC suspension and with samples. In the presence of beta-MSP, RBCs become agglutinated, providing a test simple for forensic semen identification.
authors.affiliation: Department of Forensic Medicine, Tokai University School of Medicine, Kanagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yukawa
authors.forename: M
authors.initials: M
authors.lastname: Osawa
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Takahama
authors.forename: S
authors.initials: S
authors.lastname: Takeichi
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology; D000818:Animals; D018033:Antibodies, Bispecific / Q000737:chemistry*; D000918:Antibody Specificity; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D018796:Immunoconjugates / Q000737:chemistry*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry; D008297:Male; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis* / Q000276:immunology*; D011817:Rabbits; D012661:Semen / Q000276:immunology*; D029589:Seminal Plasma Proteins; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000017:ABO Blood-Group System; D018033:Antibodies, Bispecific; D018796:Immunoconjugates; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D012710:Serum Albumin, Bovine