KLP38B: a mitotic kinesin-related protein that binds PP1.
We have identified a new member of the kinesin superfamily in Drosophila, KLP38B (kinesin-like protein at 38B). KLP38B was isolated through its two-hybrid interaction with the catalytic subunit of type 1 serine/threonine phosphoprotein phosphatase (PP1). We demonstrate that recombinant KLP38B and PP1 associate in vitro. This is the first demonstration of direct binding of a kinesin-related protein to a regulatory enzyme. Though most closely related to the Unc-104 subfamily of kinesin-related proteins, KLP38B is expressed only in proliferating cells. KLP38B mutants show cell proliferation defects in many tissues. KLP38B is required for normal chromatin condensation as embryos from KLP38B mutant mothers have undercondensed chromatin at metaphase and anaphase. This is the first time that a kinesin-related protein has been shown to have such a role. Incomplete lethality of a strong KLP38B allele suggests partial redundancy with one or more additional kinesin-related proteins.
Alphey
L
L
School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK. Luke.Alphey@man.ac.uk
Parker
L
L
Hawcroft
G
G
Guo
Y
Y
Kaiser
K
K
Morgan
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002455:Cell Division; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D029721:Drosophila Proteins; D005260:Female; D005298:Fertility; D015971:Gene Expression Regulation, Enzymologic; D016547:Kinesins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008938:Mitosis / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D009928:Organ Specificity; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D029721:Drosophila Proteins; D012333:RNA, Messenger; C106739:neb protein, Drosophila; D010749:Phosphoprotein Phosphatases; D016547:Kinesins