title: Identification by polymerase chain reaction and hybridization with sequence-specific oligoprobes and complete exon-2 cDNA sequencing of a new DRB1 (DRB1*1131) allele.
abstract: HLA-DRB1 is the most polymorphic gene described so far, and their encoded molecules disclose a major role in allogeneic responses. We describe in this report a new DRB1 allele in a Spanish Caucasian bone marrow donor, initially defined by PCR-SSO as a DRB1*11-like allele. Complete exon 2 cDNA-sequencing reveled that this allele was identical to DRB1*1119 except for a single substitution at position 178, which generates an amino acid change (Tyr-His) at position 60. This residue is shared by several DRB1*14 subtypes and DRB1*0808. The new allele was officially named DRB1*1131.
authors.affiliation: Department of Histocompatibility, Regional Transfusion Centre, Madrid, Spain. ctm@bitmailer.net
authors.forename: A
authors.initials: A
authors.lastname: Balas
authors.forename: S
authors.initials: S
authors.lastname: Santos
authors.forename: F
authors.initials: F
authors.lastname: Garciá-Sánchez
authors.forename: C
authors.initials: C
authors.lastname: Garciá-Escribano
authors.forename: J L
authors.initials: JL
authors.lastname: Merino
authors.forename: J L
authors.initials: JL
authors.lastname: Vicario
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002461:Cell Line, Transformed; D018076:DNA, Complementary; D005091:Exons; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D015345:Oligonucleotide Probes