The chicken adenosine receptor 2B gene is regulated by v-myb.
The retroviral oncogene v-myb is a mutated and truncated version of the c-myb proto-oncogene and encodes a transcription factor (v-Myb) that specifically transforms myelomonocytic cells. v-Myb is thought to transform myelomonocytic cells by affecting the expression of specific target genes, most of which as yet remain unknown. To identify novel v-Myb regulated genes we have employed 'differential display', using a myelomonocytic chicken cell line that expresses a conditional version of v-Myb. Here we describe the identification of the gene encoding the A2b adenosine receptor, a member of the seven transmembrane receptor superfamily, as a v-Myb target gene. Our results provide the first evidence that v-Myb directly regulates a gene encoding a membrane receptor and establish a link between Myb function and adenosine receptor signaling.
Worpenberg
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S
Hans-Spemann-Laboratory, Max-Planck-Institute for Immunobiology, Freiburg, Germany.
Burk
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O
Klempnauer
K H
KH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D020626:Oncogene Proteins v-myb; D011518:Proto-Oncogene Proteins / Q000502:physiology; D020598:Proto-Oncogene Proteins c-myb; D018047:Receptors, Purinergic P1 / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic / Q000502:physiology*; D015534:Trans-Activators / Q000502:physiology
D020626:Oncogene Proteins v-myb; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D018047:Receptors, Purinergic P1; D015682:Retroviridae Proteins, Oncogenic; D015534:Trans-Activators