[Quality control and cytogenetic analysis of human pre-embryos fertilized and cultured in vitro].
For the purpose to evaluate in vitro culture conditions of human preembryos, the efficacy of conventional culture and co-culture systems on embryonic development and genetic disorders was studied. Firstly, the development of cultured mouse embryos grown in standard media (Whitten's, GPM, HFT and Ham F10) or in HFT medium with different helper cell layers was compared. Embryonic growth was substantially reduced during in vitro culture, demonstrably by impaired cell proliferation, compared with in vivo controls. In in vitro fertilization and culture condition, SCEs of blastocysts were significantly increased. Development in co-culture with the feeder layers was notably better than in standard media. These results suggest that human preembryos could be rescued by the use of helper cells. Increased developmental rates and the cell numbers of blastocysts were the most evident morphological features of human preembryos that developed in co-culture with uterine luminal epithelial cells. However mosaicism may be caused by in vitro culture conditions and its onset may indicate when a disturbance in the embryonic development has occurred. It is advisable to perform further research into the mechanism of feeder cell-embryo interaction for understanding the optimal conditions of embryonic development in vitro.
Kubo
H
H
First Department of Obstetrics and Gynecology Toho University School of Medicine, Tokyo, Japan.
Abe
Y
Y
Nakano
Y
Y
Sasabe
Y
Y
Nakano
H
H
Ikenaga
H
H
Uchiide
I
I
Hashida
E
E
Suga
M
M
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005314:Embryonic and Fetal Development; D004848:Epithelium; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D051379:Mice; D009030:Mosaicism; D011786:Quality Control; D012099:Reproductive Techniques; D014599:Uterus
D003470:Culture Media