title: Some effects of adding p-LH in defined amounts to purified p-FSH to modify FSH/LH ratios during the superovulatory treatment of anestrous ewes.
abstract: Nonlactating Leccese ewes (n = 61) were used during seasonal anestrus to investigate the effects on ovarian response and embryo production of adding defined amounts of p-LH to purified p-FSH as well as decreasing the FSH/LH ratio during treatment. The ewes were synchronized with FGA-impregnated intravaginal pessaries for 9 days and prostaglandin F2 alpha (Cloprostenol) injected on the seventh day. They were divided into six treatment groups in a 3 x 2 factorial design: three amounts of purified p-LH (100, 50 or 25% equivalent to 525, 262 or 131 IU p-LH) x 2 regimen of p-FSH and p-LH administration (constant or decreasing FSH/LH ratio). Each ewe received a total of 525 IU p-FSH at a decreasing dose, twice daily over a 3-day period. Group I (n = 11), Group II (n = 10) and Group III (n = 10) were treated with p-FSH supplemented with p-LH at 100%, 50% and 25%, respectively, of p-FSH dose and a constant FSH/LH ratio throughout the treatment period. Group IV (n = 10), Group V (n = 10) and Group VI (n = 10) were treated with p-FSH supplemented with p-LH at 100%, 50% and 25%, respectively, of p-FSH dose but with a decreasing FSH/LH ratio over the 3 days of the treatment: 1.7-0.86-0.43 for Group IV; 3.4-1.7-0.86 for Group V; 6-3-1.5 for Group VI. Embryos were flushed surgically on Day 6 after estrus. The ovulation rate did not differ among the groups (8-12.8). Superovulation with 100% p-LH and decreasing the FSH/LH ratio (Group IV) resulted in: (i) the highest ova recovery (9.8 +/- 1.7), and this was significantly different (P < 0.05) from the 25% p-LH treated group (Group VI; 5.0 +/- 1.7), (ii) the highest fertilization rate (90.6 +/- 9.2%), with a significant (P < 0.01) difference compared with the constant ratio regimen (Group I; 62.6 +/- 8.3%); (iii) the highest transferable embryo yield (6.4 +/- 1.1), differing significantly (P < 0.01) from Group VI (2.2 +/- 1.1) and Group I (2.7 +/- 1.0). It is concluded that decreasing the amount of p-LH added to purified p-FSH did not improve the superovulatory response of ewes during the anestrous period. Transferable embryo production was significantly improved when ewes were treated with p-LH equivalent to 100% p-FSH, with the FSH/LH ratio decreasing during treatment.
authors.affiliation: Dipartimento di Produzione Animale, Università degli Studi di Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: D'Alessandro
authors.forename: G
authors.initials: G
authors.lastname: Martemucci
authors.forename: M A
authors.initials: MA
authors.lastname: Colonna
authors.forename: C
authors.initials: C
authors.lastname: Cafueri
authors.forename: F
authors.initials: F
authors.lastname: Toteda
mesh_terms: D000780:Anestrus / Q000502:physiology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000032:analysis / Q000494:pharmacology*; D011247:Pregnancy; D018873:Pregnancy Rate; D011897:Random Allocation; D012756:Sheep / Q000196:embryology / Q000502:physiology*; D013480:Superovulation / Q000187:drug effects* / Q000502:physiology
chemical_list: D004338:Drug Combinations; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone