Rapid chromosomal analysis of germ-line cells by FISH: an investigation of an infertile male with large-headed spermatozoa.
A rapid fluorescence in-situ hybridization (FISH) technique was used for direct chromosomal analysis on germ cells from an infertile male with large-headed spermatozoa. The interphase chromosomes were fluorescently-labelled using an extremely bright cyanine dye during a 5-15 min FISH procedure. Germ cells were analysed using a battery of chromosome-specific DNA probes in several consecutive rapid FISH experiments. It was found that the majority of large-headed spermatozoa contained a diploid chromosome number probably due to errors in meiosis I or II divisions, whereas the majority of spermatozoa with normal sized heads are haploid and may be utilized for selective in-vitro fertilization procedures. Rapid FISH may be useful for the detection of major chromosomal aneuploidies in germ cells as an alternative technique to standard or multicolour FISH, and may find an additional application for the chromosomal analysis of human preimplantation embryos.
Yurov
Y B
YB
National Research Center of Mental Health, RAMS, Moscow, Russia.
Saias
M J
MJ
Vorsanova
S G
SG
Erny
R
R
Soloviev
I V
IV
Sharonin
V O
VO
Guichaoua
M R
MR
Luciani
J M
JM
D000328:Adult; D000782:Aneuploidy; D015342:DNA Probes; D004171:Diploidy; D006238:Haploidy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007248:Infertility, Male / Q000235:genetics* / Q000473:pathology*; D008297:Male; D013094:Spermatozoa / Q000002:abnormalities* / Q000648:ultrastructure*
D015342:DNA Probes