Primary structure and bioactivity of bullfrog calcitonin.
Calcitonin was isolated from the bullfrog, Rana catesbeiana, and the first amino acid sequence of an amphibian calcitonin was determined to be Cys-Ser-Gly-Leu-Ser-Thr-Cys-Ala-Leu-Met-Lys-Leu-Ser-Gln-Asp-Leu-His- Arg-Phe-Asn-Ser-Tyr-Pro-Arg-Thr-Asn-Val-Gly-Ala-Gly-Thr-Pro-NH2. Some portions of this sequence are specific to bullfrog calcitonin, and other portions are similar both to teleost calcitonins and to mammalian calcitonins. Administration of 5 pmol of bullfrog calcitonin to rats revealed a hypocalcemic potency similar to that of salmon calcitonin, at least for the first 3 hr.
Yoshida
A
A
Faculty of Science, Kanazawa University, Ishikawa, 927-05, Japan.
Kaiya
H
H
Takei
Y
Y
Watanabe
T X
TX
Nakajima
K
K
Suzuki
N
N
Sasayama
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000737:chemistry* / Q000494:pharmacology*; D002118:Calcium / Q000097:blood; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D011892:Rana catesbeiana; D051381:Rats; D017385:Sequence Homology
C028815:salmon calcitonin; D002116:Calcitonin; D002118:Calcium