[Determination of the genotoxicity of fullerene C60 and fullerol using the method of somatic mosaics on cells of Drosophila melanogaster wing and SOS-chromotest].
Genotoxicity of fullerene C60 was been determined in a prokaryotic in vitro test and in an eukaryotic in vivo system. The SOS chromotest of fullerene C60 in the Escherichia coli strain PQ37 revealed no genotoxicity either with or without activation of the rat liver homogenate. To perform the somatic mutation and recombination genotoxicity test (SMART) on somatic wing cells, Drosophila melanogaster larvae were grown on a standard medium with or without fullerene dope. No statistically significant differences were observed at the same fullerene concentrations in the SOS chromotest (0.45 micrograms/ml). Only at the highest possible fullerene concentration of 2.24 micrograms per 1 ml medium, a slight genotoxic effect was observed in wing cells. Fullerol demonstrates no mutagenic effect at a concentration of 2.46 mg/ml.
Zakharenko
L P
LP
Zakharov
I K
IK
Vasiunina
E A
EA
Karamysheva
T V
TV
Danilenko
A M
AM
Nikiforov
A A
AA
D000818:Animals; D000998:Antiviral Agents / Q000633:toxicity*; D002244:Carbon / Q000633:toxicity*; D015197:Carcinogenicity Tests; D004331:Drosophila melanogaster / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D037741:Fullerenes; D017320:HIV Protease Inhibitors / Q000633:toxicity*; D007814:Larva; D009030:Mosaicism; D009152:Mutagenicity Tests; D051381:Rats; D011995:Recombination, Genetic; D013014:SOS Response, Genetics; D014921:Wings, Animal / Q000166:cytology
D000998:Antiviral Agents; D037741:Fullerenes; D017320:HIV Protease Inhibitors; D002244:Carbon; C069837:fullerene C60
Opredelenie genotoksichnosti fullerena C60 i fullerola metodom somaticheskikh mozaikov na kletkakh kryla Drosophila melanogaster i v SOS-khromotest.