title: Decreased kidney uptake of technetium-99m-labelled Fab' fragments in ovarian carcinoma bearing nude mice using a cleavable chelator.
abstract: A new 99mTc labelling method using a cleavable chelator, RP-1, was developed. In this study Balb/c mice with ovarian carcinoma xenografts received various Fab' fragments labelled with 99mTc either directly or via RP-1. Kidney uptake was significantly lower for the RP-1 linked conjugates. Tumour uptake showed no significant differences between RP-1 conjugates and directly labelled preparations. In conclusion, with the use of the cleavable linker RP-1, kidney uptake can be reduced significantly resulting in a lower radiation dose to the kidneys.
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital Nijmegen, The Netherlands.
authors.forename: J G
authors.initials: JG
authors.lastname: Tibben
authors.forename: L F
authors.initials: LF
authors.lastname: Massuger
authors.forename: O C
authors.initials: OC
authors.lastname: Boerman
authors.forename: R A
authors.initials: RA
authors.lastname: Claessens
authors.forename: G F
authors.initials: GF
authors.lastname: Borm
authors.forename: K Y
authors.initials: KY
authors.lastname: Pak
authors.forename: E B
authors.initials: EB
authors.lastname: Koenders
authors.forename: F H
authors.initials: FH
authors.lastname: Corstens
mesh_terms: D000818:Animals; D019540:Area Under Curve; D002614:Chelating Agents; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism* / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D010051:Ovarian Neoplasms / Q000378:metabolism*; D011829:Radiation Dosage; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D002614:Chelating Agents; D007140:Immunoglobulin Fab Fragments; D015609:Organotechnetium Compounds