Oxidation of erythrocyte protein and lipid, and hemolysis in rabbit red blood cells treated with benzo[a]pyrene or adriamycin.
A number of free-radical-generating carcinogens catalyze the oxidative modification of macromolecules. Malondialdehyde (MDA), carbonyl content, alanine formation, and hemolysis were used as biomarkers of oxidative stress, and were determined in rabbit erythrocytes treated in vitro with benzo[a]pyrene or adriamycin. MDA and carbonyl content were significantly increased in a concentration-dependent manner by carcinogens. Alanine formation was also increased in a concentration-dependent manner in rabbit erythrocytes treated with carcinogens. Hemolysis occurred in erythrocytes treated with benzo[a]pyrene (540 microM) or adriamycin (300 microM) between 4 and 8 h of incubation, respectively. The hemolysis pattern correlated with increases in MDA, carbonyl content, and alanine formation. These data indicate that lipid peroxidation as measured by MDA may be the most sensitive indicator for oxidative stress in erythrocytes. Hemolysis could thus be applicable to free-radical-induced cellular damage as an alternative biomarker of oxidative stress.
Lee
S K
SK
Division of Toxicology, School of Pharmacy, Sung Kyun Kwan University, Kyunggi-Do, Suwon, Korea.
Lee
B M
BM
D000409:Alanine / Q000378:metabolism; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D015415:Biomarkers; D002273:Carcinogens / Q000633:toxicity*; D002478:Cells, Cultured; D004317:Doxorubicin / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D000903:Antibiotics, Antineoplastic; D015415:Biomarkers; D002273:Carcinogens; D001564:Benzo(a)pyrene; D008315:Malondialdehyde; D004317:Doxorubicin; D000409:Alanine