In search of a function for the Ras-like GTPase Rap1.
Rap1 (Krev-1) is a small GTPase first identified as a transformation suppressor of K-ras. This GTPase is very similar to Ras, particularly in the effector region, but its function is still elusive. Recent progress in the search for Rap1 function has come from the development of a novel assay to measure Rap1 activation. Using this assay activation of Rap1 was observed in human platelets and neutrophils after stimulation with various agonists. We speculate that Rap1 plays a role in one of the specialised functions of these cells.
Bos
J L
JL
Laboratory for Physiological Chemistry, Utrecht University, The Netherlands.
Franke
B
B
M'Rabet
L
L
Reedquist
K
K
Zwartkruis
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000201:enzymology; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000201:enzymology; D020668:rap GTP-Binding Proteins; D018631:ras Proteins
D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins; D018631:ras Proteins