title: High performance liquid chromatography of carbon-11 labeled thymidine and its major catabolites for clinical PET studies.
abstract: The identity and pharmacology of the rapidly formed radiolabeled metabolites formed following i.v. injection of C-11 thymidine (TdR) labeled in the 5-methyl-position are being studied in animal models and patients with cancer. A high performance liquid chromatographic (HPLC) procedure has been developed for the separation of these metabolites including thymine, dihydrothymine, beta-ureidoisobutyric acid, and beta-aminoisobutyric acid from plasma and tissue extracts. Information obtained regarding the pharmacokinetics of the metabolites are being used to generate mathematical models for C-11 TdR incorporation rates into DNA.
authors.affiliation: Department of Radiology and Radiological Sciences, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.
authors.forename: P S
authors.initials: PS
authors.lastname: Conti
authors.forename: J
authors.initials: J
authors.lastname: Hilton
authors.forename: D F
authors.initials: DF
authors.lastname: Wong
authors.forename: M M
authors.initials: MM
authors.lastname: Alauddin
authors.forename: R F
authors.initials: RF
authors.lastname: Dannals
authors.forename: H T
authors.initials: HT
authors.lastname: Ravert
authors.forename: A A
authors.initials: AA
authors.lastname: Wilson
authors.forename: J H
authors.initials: JH
authors.lastname: Anderson
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000097:blood / Q000000981:diagnostic imaging; D002250:Carbon Radioisotopes / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005909:Glioblastoma / Q000097:blood / Q000000981:diagnostic imaging; D006801:Humans; D008297:Male; D013936:Thymidine / Q000097:blood / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed
chemical_list: D002250:Carbon Radioisotopes; D013936:Thymidine