Cyclin-dependent kinase 6 is the principal target of p27/Kip1 regulation of the G1-phase traverse in 1,25-dihydroxyvitamin D3-treated HL60 cells.
Control of cell proliferation remains of intense interest in cancer research. In the 1,25-dihydroxyvitamin D3 HL60 cell system, G1 arrest has been shown to be mediated by elevated levels of p27/Kip1 protein. We show here that the main target of the elevated p27/Kip1 in this system is cyclin-dependent kinase (Cdk) 6. The activity of Cdk2 is also down-regulated, and this is associated with altered and reduced levels of cyclin E in the kinase complex. Paradoxically, the kinase activity of Cdk4 is elevated, in spite of an almost complete G1 block. These data show that the functions of Cdk4 and Cdk6 are not redundant and that Cdk6 and Cdk2 activities are regulated by 1,25-dihydroxyvitamin D3.
Wang
Q M
QM
Department of Pathology and Laboratory Medicine, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103, USA.
Luo
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X
Studzinski
G P
GP
D002117:Calcitriol / Q000494:pharmacology*; D018797:Cell Cycle Proteins; D051358:Cyclin-Dependent Kinase 4; D051361:Cyclin-Dependent Kinase 6; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000502:physiology; D016193:G1 Phase / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000187:drug effects / Q000502:physiology*; D011518:Proto-Oncogene Proteins; D025521:Tumor Suppressor Proteins
D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D011518:Proto-Oncogene Proteins; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D017346:Protein Serine-Threonine Kinases; C495900:CDK4 protein, human; C495911:CDK6 protein, human; D051358:Cyclin-Dependent Kinase 4; D051361:Cyclin-Dependent Kinase 6; D018844:Cyclin-Dependent Kinases; D002117:Calcitriol