Respirometer for small samples with automatic registration.
The respiration chamber containing the sample is a small glass test tube with a closure of KOH solution. During registration the gas volume is kept constant by adjusting the pressure through a feedback mechanism: a microscope projects the picture of the meniscus of KOH onto a photodiode connected to a pen recorder with a vertically moving pen. A levelling bulb with water mounted on the pen transmits the pressure adjustments to the KOH solution through a water filled tubing. The accuracy is +/-0.1 mm water column (1 Pa) and the minimum air volume is 10 nl, so that an oxygen consumption down to 1 pl can be seen.
Floto
F
F
Department of Plant Physiology, University of Copenhagen, Denmark. floto@pfa.molbio.ku.dk
D002245:Carbon Dioxide / Q000378:metabolism; D008365:Manometry / Q000295:instrumentation* / Q000379:methods; D008853:Microscopy; D010101:Oxygen Consumption / Q000502:physiology*; D018532:Pisum sativum / Q000378:metabolism; D010788:Photosynthesis; D018517:Plant Roots / Q000378:metabolism*
D002245:Carbon Dioxide