Acquired resistance to experimental autoimmune encephalomyelitis is independent of V beta usage.
In Lewis rats, activated encephalitogenic T-helper cells elicit a single bout of experimental autoimmune encephalomyelitis (EAE). Recovery from EAE is marked by reduced susceptibility to disease reinduction. The purpose of this study was to determine whether a dominant expression of V beta gene segments by encephalitogenic T cells was required for development of recovery-associated resistance. Several polyclonal and monoclonal T cell lines were derived from Lewis rats sensitized with R72-86, a synthetic peptide representing the 72- to 86-amino-acid sequence of rat myelin basic protein (RMBP). The results revealed broad heterogeneity among encephalitogenic T cells specific for R72-86 in regard to V beta expression and CDR3 sequence. Encephalitogenic clones exclusively bearing either V beta 4 or V beta 10 TCR or polyclonal T cells bearing heterogeneous TCR transferred EAE to recipient rats and elicited resistance to EAE as revealed by subsequent challenge with guinea pig (GP)MBP in complete Freund's adjuvant (CFA). Nonpathogenic V beta 3+ and V beta 8.6+ clones specific for the 68-86 and 55-66 regions of MBP, respectively, did not elicit effective protection from EAE. These data indicate that induction of postrecovery resistance to EAE does not depend upon a particular V beta usage.
Johnson
B D
BD
Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina, USA.
Nardella
J P
JP
McConnell
T J
TJ
Mannie
M D
MD
D019264:Adoptive Transfer; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D006168:Guinea Pigs; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D004676:Myelin Basic Protein; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA