Laboratory diagnosis of respiratory virus infections in 24 hours by utilizing shell vial cultures.
Immunofluorescence staining of centrifugation-enhanced shell vial (SV) cultures for respiratory viruses (RV) after 24 h of incubation, rather than the more commonly prescribed times of 48 h and 5 days, allowed for the detection of 77% of the RV-positive specimens that would ordinarily not have been detected as positive until 48 h. Staining SVs at 24 h also permitted earlier detection of viruses that were missed by rapid antigen detection methods.
Engler
H D
HD
Clinical Pathology Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA. hengler@nih.gov
Preuss
J
J
D006801:Humans; D012137:Respiratory System / Q000821:virology; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D013997:Time Factors; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology