[Value of the Remedi chromatography automate for the analytic diagnosis of drug poisoning].
The aim of this study was to evaluate the liquid chromatographic system Remedi (Biorad) in comparison with traditional immunological and colorimetric methods, for the diagnosis of acute drug overdose. 469 blood samples and 95 stomach cleaning liquid samples have been analysed during 1995. The usual toxicologic analysis was composed of the benzodiazepines, tricyclic antidepressants and barbiturates research. Ethanol, meprobamate and acetaminophen assays were performed only on physician's request. Only three pharmacological classes can be analysed both with immunological methods and Remedi: benzodiazepines, tricyclic antidepressants and barbiturates. Remedi has been found to be less sensitive than immunological method for benzodiazepines, it sometimes gave false negative results for barbiturates, but it was very efficient for antidepressants. Remedi often identified drugs other than the 3 previous classes: sedatives, antipsychotic, beta-blockers, antiarythmics. Furthermore these drugs are of clinical importance due to the fact that they are able to modify the symptomatology. In every case Remedi was able to give an estimation of the blood concentration of the toxic molecule matched. Remedi can not replace traditional methods but is a good complementary tool, available in emergency. This is particularly useful when clinical signs do not correspond to the toxics suspected by questioning the patient or relatives.
Manchon
M
M
Laboratoire des urgences biochimiques et toxicologiques, Pierre-Bénite.
Mialon
A
A
Berny
C
C
Baltassat
P
P
D000929:Antidepressive Agents, Tricyclic / Q000032:analysis / Q000097:blood; D001463:Barbiturates / Q000032:analysis / Q000097:blood; D001569:Benzodiazepines / Q000032:analysis / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D005750:Gastric Juice / Q000737:chemistry; D006801:Humans; D007256:Information Systems; D011041:Poisoning / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism
D000929:Antidepressive Agents, Tricyclic; D001463:Barbiturates; D001569:Benzodiazepines
Intérêt de l'automate de chromatographie Remedi pour le diagnostic analytique des intoxications médicamenteuses.