title: Studies on the translation mechanism of subgenomic RNA of potato leafroll virus.
abstract: The expression of open reading frames located on the subgenomic RNA (sgRNA) has been studied in an in vitro transcription and translation system. The obtained results indicate: a) translation of sgRNA occurs according to the scanning model since the insertion of palindrome (delta G0 = -61 kcal/mol) prevents the initiation of translation; b) ORF6 is translated by suppression of the stop codon separating ORF4 from ORF6 and the presence of suppressor tRNA is necessary for the readthrough; c) the presence of leader sequence of sgRNA (212 nucleotides) decreases the translation efficiency of ORFs located downstream and it affects the ratio of products of ORF4 and ORF5; d) 3'UTR does not influence on an expression of genes located on the sgRNA.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Juszczuk
authors.forename: W
authors.initials: W
authors.lastname: Zagórski-Ostoja
authors.forename: D M
authors.initials: DM
authors.lastname: Hulanicka
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics*; D011198:Solanum tuberosum / Q000821:virology*
chemical_list: D012367:RNA, Viral