title: The efficacy of trypsin for disinfection of in vitro fertilized bovine embryos exposed to bovine herpesvirus 1.
abstract: Bovine cumulus-oocyte complexes (COC) or in vitro fertilized (IVF) embryos were exposed to bovine herpesvirus 1 (BHV-1) during in vitro maturation or co-culture with uterine tubal cells, respectively. Trypsin, at a concentration of 0.25%, was applied (for approximately 90 s) to disinfect either COC or cumulus-free oocytes (CFO) 18 h after insemination, or on day 7 to embryos resulting from infected oocytes. In total, virus was not detected in 71% of 93 samples containing 233 embryos exposed to BHV-1 and trypsin treatment. BHV-1 was detected in 14% and 54% of samples containing a single embryo and five embryos, respectively. In corresponding groups of embryos exposed to BHV-1, then washed but not treated with trypsin (70 samples), 85% and 96% of samples containing one embryo and pooled embryos, respectively, were positive for the virus. There was no effect of trypsin treatment on the development of IVF-embryos. It is concluded that IVF-generated embryos have a greater tendency to carry BHV-1 after experimental exposure to the virus than IVF uterine stage embryos, and that they are more difficult to disinfect by means of the standard trypsin treatment use.
authors.affiliation: Agriculture and Agri-Food Canada, Animal Diseases Research Institute, Nepean, Ont., Canada.
authors.forename: A
authors.initials: A
authors.lastname: Bielanski
authors.forename: C
authors.initials: C
authors.lastname: Lutze-Wallace
authors.forename: T
authors.initials: T
authors.lastname: Sapp
authors.forename: L
authors.initials: L
authors.lastname: Jordan
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000196:embryology* / Q000821:virology; D002418:Cattle Diseases / Q000517:prevention & control; D018920:Coculture Techniques / Q000379:methods / Q000662:veterinary; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004203:Disinfection / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects / Q000821:virology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary; D007242:Herpesvirus 1, Bovine / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D009865:Oocytes / Q000166:cytology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D014357:Trypsin / Q000494:pharmacology*; D014599:Uterus / Q000166:cytology
chemical_list: D004279:DNA, Viral; D014357:Trypsin