title: The radiographic stage of giant cell tumor related to stromal cells' proliferation. Tissue cultures in 13 cases.
abstract: The clinical behavior of giant cell tumor is related to the radiological appearance. To test the hypothesis that in vitro proliferation of the neoplastic stromal cell population of giant cell tumors is related to the radiological appearance, this study was undertaken. A prospective analysis of the cells migrating from 13 consecutive tumors was conducted. Growth curves and population doubling-times (PDT) for first and fifth passages were calculated and alkaline phosphatase levels were measured and compared to preoperative radiographic staging. A strong negative correlation was found between PDT and the radiographic stage. Tumors in stages I and II (low aggressiveness) were found to have an average cell population doubling-time of 11 (SD 2.2) days, while those in stage III (high aggressiveness) showed a doubling-time of 6 (SD 2.2) days. Low alkaline phosphatase activity was noted in all cultures, a finding consistent with the putative preosteoblastic potential of these stromal cells. This putative origin is also indicated by the differentiation response to retinoic acid. The findings suggest that the in vitro proliferation of the mononuclear stromal cell population of giant cell tumors is related to the radiographic stage and may predict the clinical behavior of these tumors.
authors.affiliation: Department of Orthopedics, Mount Sinai School of Medicine, New York, NY, USA.
authors.forename: D
authors.initials: D
authors.lastname: Robinson
authors.forename: M M
authors.initials: MM
authors.lastname: Lewis
authors.forename: Z
authors.initials: Z
authors.lastname: Nevo
authors.forename: S
authors.initials: S
authors.lastname: Kenan
authors.forename: T A
authors.initials: TA
authors.lastname: Einhom
mesh_terms: D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D014408:Biomarkers, Tumor; D001706:Biopsy; D001859:Bone Neoplasms / Q000000981:diagnostic imaging* / Q000201:enzymology / Q000473:pathology; D002452:Cell Count; D002455:Cell Division; D002465:Cell Movement; D002675:Child, Preschool; D005269:Femur / Q000000981:diagnostic imaging / Q000201:enzymology / Q000473:pathology; D018212:Giant Cell Tumor of Bone / Q000000981:diagnostic imaging* / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007085:Ilium / Q000000981:diagnostic imaging / Q000201:enzymology / Q000473:pathology; D008663:Metacarpus / Q000000981:diagnostic imaging / Q000201:enzymology / Q000473:pathology; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011446:Prospective Studies; D011859:Radiography; D017154:Stromal Cells / Q000201:enzymology / Q000473:pathology; D013977:Tibia / Q000000981:diagnostic imaging / Q000201:enzymology / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D018809:Proliferating Cell Nuclear Antigen; D000469:Alkaline Phosphatase