title: Small peptide libraries: combinatorial split-mix synthesis followed by combinatorial amino acid analysis of selected variants.
abstract: Peptides from small combinatorial libraries, covalently attached to polymeric TentaGel beads, can be directly sequenced using amino acid analysis. For libraries with restricted diversity, generated by the split-mix synthesis method, the amino acids on a selected single bead identified by pre-column derivatization with o-phthaldialdehyde (OPA) correlate directly with the sequence of a given peptide. This is shown on a tripeptide (343 different compounds) and a tetrapeptide (4096 different compounds) library. This method allows for rapid peptide sequence determination without relying on complex encoding strategies.
authors.affiliation: Institute of Organic Chemistry, J.W. Goethe-University of Frankfurt, Frankfurt/Main, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Weinberger
authors.forename: E
authors.initials: E
authors.lastname: Lichte
authors.forename: C
authors.initials: C
authors.lastname: Griesinger
authors.forename: B
authors.initials: B
authors.lastname: Kutscher
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D008863:Microspheres; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols; D017421:Sequence Analysis / Q000379:methods*
chemical_list: D000596:Amino Acids; D019151:Peptide Library; D010455:Peptides; D011092:Polyethylene Glycols