Molecular cloning of human RP105.
RP105 is a 105-kDa type I membrane protein of the leucine-rich repeat (LRR) family. Anti-RP105 sensitizes B cells to antigen-receptor-mediated apoptosis, but protects B cells from radiation-induced apoptosis and stimulates B cell proliferation. The sequence of the mouse RP105 has been reported. Here, we report the characterization of the human RP105. The 2.6-kb cDNA encodes a protein of 661 amino acids which displays 78% homology with mouse RP105. The 22 LRR and the 9 potential N-linked glycosylation sites within the extracellular region are conserved. While previous studies have shown that RP105 is expressed on surface IgM+IgD+2 B cells in mice, human RP105 was shown to be expressed on all subsets of mature B cells and dendritic cells. Human RP105 gene was mapped to the long arm of chromosome 5, where numerous cytokines and receptors have been localized.
Fugier-Vivier
I
I
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
de Bouteiller
O
O
Guret
C
C
Fossiez
F
F
Banchereau
J
J
Mattei
M G
MG
Aït-Yahia
S
S
Garcia
E
E
Lebecque
S
S
Liu
Y J
YJ
D000818:Animals; D015703:Antigens, CD; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D014066:Palatine Tonsil; D017386:Sequence Homology, Amino Acid
D015703:Antigens, CD; D000954:Antigens, Surface; C092722:CD180 protein, human; C490500:Ly78 protein, mouse; D008565:Membrane Proteins