title: Inhibition of the genotoxic effects of heterocyclic amines in human derived hepatoma cells by dietary bioantimutagens.
abstract: The effects of dietary bioantimutagens (compounds which have been shown to inhibit mutagenesis via interaction with DNA repair processes) on spontaneous and heterocyclic amine (HCA)-induced micronucleus (MN) frequencies were studied in metabolically competent human hepatoma (Hep-G2) cells. All the compounds tested (coumarin, vanillin, caffeine, tannic acid and cinnamaldehyde) caused a moderate increase of MN numbers in Hep-G2 cells at high concentrations (500 microg/ml); only tannic acid was also active at lower dose levels. In combination experiments with the HCA 2-amino-3-methylimidazo-[3,4-f]quinoline (IQ), post-treatment of the cells with bioantimutagens resulted in a pronounced (75-90%) decrease in MN. The most drastic effects were seen with vanillin, coumarin and caffeine which were active at concentrations < or = 5 microg/ml. Further experiments indicated that these compounds also attenuate the mutagenic effects of other HCAs (PhIP, MeIQ, MeIQx, Trp-P-1).
authors.affiliation: Institute of Tumor Biology and Cancer Research, University of Vienna, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Sanyal
authors.forename: F
authors.initials: F
authors.lastname: Darroudi
authors.forename: W
authors.initials: W
authors.lastname: Parzefall
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: S
authors.initials: S
authors.lastname: Knasmüller
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives / Q000494:pharmacology; D000588:Amines / Q000633:toxicity*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001547:Benzaldehydes / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D002243:Carbolines / Q000633:toxicity; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000235:genetics*; D003374:Coumarins / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects; D006571:Heterocyclic Compounds / Q000633:toxicity*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology; D007093:Imidazoles / Q000633:toxicity; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity; D011804:Quinolines / Q000633:toxicity; D014407:Tumor Cells, Cultured
chemical_list: D000588:Amines; D016587:Antimutagenic Agents; D001547:Benzaldehydes; D002243:Carbolines; D003374:Coumarins; D006571:Heterocyclic Compounds; D047348:Hydrolyzable Tannins; D007093:Imidazoles; D009153:Mutagens; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; D002110:Caffeine; D000171:Acrolein; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; C030123:coumarin; C100058:vanillin; C036989:2-amino-3,4-dimethylimidazo(4,5-f)quinoline; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole; C012843:cinnamaldehyde