[Energy metabolism of the human cornea in various culture systems].
BACKGROUND
There are two well known systems to culture human corneas prior to transplantation. First, corneal storage at 4 degrees C especially in Optisol medium. Second, organ-culture at physiological temperatures in a modified minimal essential medium (MEM). In the cold storage system the number of endothelial cells after storage might be overestimated because the damaged cells are not able to leave the monolayer. It has been supposed that the lack in energy recruitment is the main reason for that, but has not been proven yet. It was the purpose of this study to describe the energy status of the human cornea after storage in both systems.


METHODS
32 human corneas were investigated. They were stored for 7 days in Optisol, and for 7 days in MEM plus 1 day in MEM supplemented with 5% dextran 500 and 12 days in modified MEM plus 1 day in MEM supplemented with 5% dextran 500. The endothelial cell density (ECD) as well as the hydration were determined. Glucose, lactate, ATP, ADP and AMP were measured to reflect the energy status.


RESULTS
Hydration was comparable in all three groups. ECD was slightly higher in Optisol stored corneas, although the amount of damaged cells was much higher. Optisol stored corneas showed a severe anaerobic situation, especially lacate concentrations were increased. In contrast ATP and ADP concentrations were twice as high in MEM than in Optisol stored corneas.


CONCLUSIONS
The severe anaerobic situation in Optisol stored corneas leads to a lack in energy recruitment. This reduces the ability of cell function (mitosis) and the function of the monolayer (migration, elimination). Whether these changes are reversible after transplantation has to be determined in future.
Redbrake
C
C
Augenklinik, RWTH Aachen.
Salla
S
S
Frantz
A
A
Reim
M
M
D000227:Adenine Nucleotides / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D002452:Cell Count; D002809:Chondroitin Sulfates; D045424:Complex Mixtures; D016039:Corneal Transplantation / Q000502:physiology*; D016895:Culture Media, Serum-Free; D003911:Dextrans; D004728:Endothelium, Corneal / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology*; D005839:Gentamicins; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D014882:Water-Electrolyte Balance / Q000502:physiology
D000227:Adenine Nucleotides; D001786:Blood Glucose; D045424:Complex Mixtures; D016895:Culture Media, Serum-Free; D003911:Dextrans; D005839:Gentamicins; D019344:Lactic Acid; D002809:Chondroitin Sulfates; C075736:Optisol
Untersuchungen zum Energiestoffwechsel der humanen Hornhaut in verschiedenen Kultursystemen.