title: Intracellular calcium mobilization following prostaglandin receptor activation in human ciliary muscle cells.
abstract: OBJECTIVE
  To investigate the presence of specific prostaglandin receptors in primary cultures of human ciliary muscle cells by measuring agonist-stimulated intracellular calcium mobilization.
  
  
  METHODS
  The ciliary muscle cells, cultured from postmortem human ciliary muscle explants, were first characterized by anti-desmin and anti-smooth muscle alpha-actin antibodies. Increase in intracellular calcium concentrations, in fura 2-AM loaded human ciliary muscle cells stimulated by prostaglandin receptor agonists, were determined with a digital fluorescence imaging system.
  
  
  RESULTS
  The resting intracellular calcium concentration in the fura 2-AM loaded cells was 60.0 +/- 6.0 nM. The threshold concentration of PG receptor agonists needed to increase the concentration of intracellular calcium was 10(-8) M. The stimulation of these cells by PGF2 alpha, 17-phenyl trinor PGE2, and U46619, the FP, EP1, and TP receptor agonists, resulted in the dose-dependent increase of intracellular calcium.
  
  
  CONCLUSIONS
  The results of the present study suggest that EP1, FP, and TP receptors are present in human ciliary muscle cells.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Louisville, KY, USA.
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: T
authors.initials: T
authors.lastname: Andom
authors.forename: P
authors.initials: P
authors.lastname: Bhattacherjee
authors.forename: C
authors.initials: C
authors.lastname: Paterson
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D000199:Actins / Q000378:metabolism; D000328:Adult; D002118:Calcium / Q000378:metabolism*; D002924:Ciliary Body / Q000187:drug effects / Q000378:metabolism*; D003893:Desmin / Q000378:metabolism; D015237:Dinoprost / Q000494:pharmacology; D015232:Dinoprostone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000378:metabolism; D016257:Fura-2 / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology; D011982:Receptors, Prostaglandin / Q000378:metabolism*; D013928:Thromboxane A2 / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: C092396:17-phenyltrinorprostaglandin E2; D000199:Actins; D003893:Desmin; D005456:Fluorescent Dyes; D011450:Prostaglandin Endoperoxides, Synthetic; D011982:Receptors, Prostaglandin; C049925:fura-2-am; D013928:Thromboxane A2; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D015237:Dinoprost; D015232:Dinoprostone; D002118:Calcium; D016257:Fura-2