title: Nucleosomal structures of c-myc promoters with transcriptionally engaged RNA polymerase II.
abstract: Organization of DNA into chromatin has been shown to contribute to a repressed state of gene transcription. Disruption of nucleosomal structure is observed in response to gene induction, suggesting a model in which RNA polymerase II (pol II) is recruited to the promoter upon reorganization of nucleosomes. Here we show that induction of c-myc transcription correlates with the disruption of two nucleosomes in the upstream promoter region. This nucleosomal disruption, however, is not necessary for the binding of pol II to the promoter. Transcriptionally engaged pol II complexes can be detected when the upstream chromatin is in a more closed configuration. Thus, upstream chromatin opening is suggested to affect activation of promoter-bound pol II rather than entry of polymerases into the promoter. Interestingly, pol II complexes are detectable in both sense and antisense transcriptional directions, but only complexes in the sense direction respond to activation signals resulting in processive transcription.
authors.affiliation: Institut für Klinische Molekularbiologie und Tumorgenetik, GSF-Forschungszentrum für Umwelt und Gesundheit, Munich, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Albert
authors.forename: J
authors.initials: J
authors.lastname: Mautner
authors.forename: J O
authors.initials: JO
authors.lastname: Funk
authors.forename: K
authors.initials: K
authors.lastname: Hörtnagel
authors.forename: A
authors.initials: A
authors.lastname: Pullner
authors.forename: D
authors.initials: D
authors.lastname: Eick
mesh_terms: D001402:B-Lymphocytes; D002461:Cell Line, Transformed; D002843:Chromatin / Q000737:chemistry; D018983:DNA Footprinting; D016259:Genes, myc / Q000235:genetics*; D018922:HL-60 Cells; D006801:Humans; D009707:Nucleosomes / Q000201:enzymology; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000032:analysis / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D012319:RNA Polymerase II