Partial restoration of inactivated ribosomes: role of the aldehyde group generated by RNA N-glycosidase in the sarcin/ricin domain of 28S rRNA in ribosome.
In this paper, we report more information on the important role of the aldehyde group in the sarcin/ricin domain of 28S rRNA in rat liver ribosome. We find D-amino acids, amino acid derivatives having free amino group and two polyamines can also partially restore the activity of cinnamomin-inactivated ribosomes. However, amino acid derivatives and a tripeptide with blocked amino group cannot. Neither sodium borohydride nor the L-amino acids can restore the activity of ribosomes inactivated by alpha-sarcin. These data demonstrate that partial restoration of the activity of the inactivated ribosome is indeed the result of the blockage of the aldehyde group. It reaches the conclusion that emergence of the active aldehyde group in the sarcin/ricin domain of 28S rRNA is one of factors that inactivate the ribosome for protein synthesis.
Cheng
L P
LP
Shanghai Institute of Biochemistry, Academia Sinica, China.
Liu
W Y
WY
D000447:Aldehydes / Q000378:metabolism; D020418:Algal Proteins; D000596:Amino Acids / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D001894:Borohydrides / Q000494:pharmacology; D004722:Endoribonucleases / Q000378:metabolism* / Q000494:pharmacology; D005656:Fungal Proteins; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D011073:Polyamines / Q000494:pharmacology; D014176:Protein Biosynthesis; D011506:Proteins / Q000494:pharmacology; D012339:RNA, Ribosomal, 28S / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D012276:Ricin / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D000447:Aldehydes; D020418:Algal Proteins; D000596:Amino Acids; D001894:Borohydrides; D005656:Fungal Proteins; D011073:Polyamines; D011506:Proteins; D012339:RNA, Ribosomal, 28S; D054790:Ribosome Inactivating Proteins, Type 2; C061930:cinnamomin, Phytophthora cinnamomi; C014801:alpha-sarcin; D013096:Spermine; C025364:sodium borohydride; D012276:Ricin; D004722:Endoribonucleases; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins; D013095:Spermidine