Quantification of cell adhesion using a spinning disc device and application to surface-reactive materials.
Quantitative analysis of cell adhesion is essential in understanding physiological phenomena and developing biotechnological applications. Electrochemical measurements demonstrated that the transport patterns associated with a spinning disc device approximate the fluid flow and mass transport fields for a disc spinning in an infinite fluid. Therefore, this device applies a linear range of forces to attached cells under uniform and constant chemical conditions at the interface. The application of this apparatus for examining cell adhesion to surface-active materials was illustrated by investigating the attachment of osteoblast-like cells to fibronectin adsorbed onto bioactive and non-reactive glasses for different chemical environments. Cells were seeded on fibronectin-coated substrates for 15 min and then subjected to detachment forces for 10 min. The number of adherent cells decreased non-linearly with applied force and the detachment profile was accurately described by a sigmoidal curve fit, as expected for a cell population with normally distributed adhesion properties.
García
A J
AJ
Department of Bioengineering, University of Pennsylvania, Philadelphia 19104, USA.
Ducheyne
P
P
Boettiger
D
D
D000327:Adsorption; D000704:Analysis of Variance; D000818:Animals; D001709:Biotechnology; D001798:Blood Proteins / Q000378:metabolism; D001859:Bone Neoplasms / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology*; D004563:Electrochemistry / Q000295:instrumentation*; D005353:Fibronectins / Q000378:metabolism*; D005898:Glass; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D012516:Osteosarcoma / Q000473:pathology; D051381:Rats; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D005353:Fibronectins