Biochemical and cytogenetical study of the mycoplasmal antigen and of the cyclophosphamide action in mammalians, in vivo. The action of some immunomodulatory antioxidants.
It was proved spectrophotometrically that Mycoplasma agalactiae antigen inoculated in vivo in sheep modifies the corresponding erythrocyte lysates reactivity toward methylene blue and neutral red and induces several types of chromosomal rearrangements. The treatment in vivo of sheep with an original preparation obtained from the Phaseolus vulgaris pods restores the erythrocyte lysates reactivity toward the two redox dyes and reduces the chromosomal abnormalities frequency induced by the mycoplasmal antigen. It was also demonstrated by optical and electronical microscopy that the Smise line mouse meiocytes exhibit chromosomal abnormalities induced by the cyclophosphamide treatment in vivo. In the case of concomitant treatment with the cyclophosphamide and C vitamin the same frequency of abnormalities was recorded as in the simple treatment with the drug.
Dumitrescu
M
M
University of Bucharest, Faculty of Biology, General Genetics and Evolution Department, Romania.
Gavrilă
V R
VR
Gavrilă
L B
LB
Gheţea
L
L
Grecu
C
C
Alexandru
G
G
Talmaci-Basalic
R
R
Stefan
M
M
Petrescu
A
A
Mihăescu
G
G
Scorpan
V
V
Bujorean
V
V
Jacota
A
A
Bucur
E
E
Pascale
F
F
Vior
C
C
Gavrilă
L
L
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D004396:Coloring Agents; D003520:Cyclophosphamide / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects*; D006131:Growth Inhibitors / Q000494:pharmacology*; D008297:Male; D008540:Meiosis / Q000187:drug effects; D008751:Methylene Blue; D051379:Mice; D009174:Mycoplasma / Q000276:immunology*; D009499:Neutral Red; D010084:Oxidation-Reduction; D010835:Phytohemagglutinins / Q000494:pharmacology; D012756:Sheep; D013090:Spermatocytes / Q000187:drug effects*; D014801:Vitamin A / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D000975:Antioxidants; D004396:Coloring Agents; D006131:Growth Inhibitors; D010835:Phytohemagglutinins; D014801:Vitamin A; D014810:Vitamin E; D009499:Neutral Red; D003520:Cyclophosphamide; D001205:Ascorbic Acid; D008751:Methylene Blue