Taurine analysis in milk and infant formulae by liquid chromatography: collaborative study.
A collaborative study was conducted on a liquid chromatographic (LC) method for determination of taurine in infant formula and milk powders. Twenty laboratories participated in the analysis of 8 blind duplicates over the range of approximately 3-60 mg/100 g sample. The method involved protein removal, conversion to the dansyl-derivative, and isocratic LC separation with UV and/or fluorescence detection. Following outlier treatment, overall mean RSDR has been estimated at 7.00% for supplemented products with a HORRAT value of 1.1. The poorer precision at endogenous levels establishes a lower limit of determination of about 5 mg/100 g. An overall mean RSDr:RSDR value of 0.7 for all products demonstrated acceptable performance.
Woollard
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DC
Ministry of Agriculture, Lynfield Food Services Centre, Auckland, New Zealand.
Indyk
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HE
D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D002853:Chromatography, Liquid; D003611:Dairy Products / Q000032:analysis; D006801:Humans; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D008892:Milk / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D030262:Soybean Proteins / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013654:Taurine / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D002364:Caseins; D030262:Soybean Proteins; D014867:Water; D013654:Taurine; C032159:acetonitrile