Regulation of androgen receptor (AR) and prostate specific antigen (PSA) expression in the androgen-responsive human prostate LNCaP cells by ethanolic extracts of the Chinese herbal preparation, PC-SPES.
As part of the study on the potential use of natural product-based combination therapy for treating prostate cancer, we have investigated the effects of a "HPLC standardized" herbal preparation, PC-SPES, on the prostate LNCaP cell line. Proliferation of the LNCaP cells was inhibited by a 4-6 day incubation with ethanolic extracts of PC-SPES. Decrease of cell growth was accompanied by a 60-70% down-regulation of the proliferating cell nuclear antigen (PCNA) and level of secreted PSA. A smaller and more variable decrease (20-40%) in the level of intracellular PSA was also observed. The PC-SPES-modulated PSA changes occurred concurrently with the decrease of AR expression, based on Western blot analysis and binding to the radioactive ligand [3H]R1881. A 60% decrease in R1881 binding occurred after a 24 h incubation with PC-SPES. These results suggest that PC-SPES negatively affects cell growth in part through its ability to modulate changes in PCNA, and may decrease PSA levels indirectly by suppressing AR expression.
Hsieh
T
T
Department of Biochemistry & Molecular Biology, New York Medical College, Valhalla 10595, USA.
Chen
S S
SS
Wang
X
X
Wu
J M
JM
D000728:Androgens; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002277:Carcinoma / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal; D000431:Ethanol; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D019684:Magnoliopsida; D008297:Male; D009376:Neoplasms, Hormone-Dependent / Q000473:pathology*; D010936:Plant Extracts / Q000494:pharmacology*; D017430:Prostate-Specific Antigen / Q000096:biosynthesis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000473:pathology*; D011944:Receptors, Androgen / Q000096:biosynthesis* / Q000235:genetics; D012997:Solvents; D014407:Tumor Cells, Cultured
D000728:Androgens; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011944:Receptors, Androgen; D012997:Solvents; C114209:herbal preparation PC-SPES; D000431:Ethanol; D017430:Prostate-Specific Antigen