Nucleotide sequence of the ermE distal flank of the erythromycin biosynthesis cluster in Saccharopolyspora erythraea.
A 7023 nucleotide BamHI fragment immediately upstream of the eryK gene of the erythromycin (Er) biosynthesis cluster in Saccharopolyspora erythraea was sequenced. Computer-assisted analysis of this sequence reveals the existence of seven ORFs that display the codon preferences typical of actinomycete genes. Six of these show homology to known genes: an esterase, a transposase, a peptidyl-prolyl cis-trans isomerase, a subtilisin inhibitor-like protein, and two genes involved in bacterial cell wall biosynthesis. All the ORFs are transcribed toward the Er biosynthetic gene cluster (in the same direction as eryK). From the predicted functions of the putative ORF products none of these genes appear to be involved in the biosynthesis of Er. The eryK gene thus most likely defines one end of the Er biosynthetic gene cluster.
Pereda
A
A
Antibacterial Discovery Research, Abbott Laboratories, Abbott Park, IL 60064, USA.
Summers
R
R
Katz
L
L
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004917:Erythromycin / Q000096:biosynthesis*; D005798:Genes, Bacterial; D006899:Mixed Function Oxygenases / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D016930:Saccharopolyspora / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004917:Erythromycin; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C078882:EryK protein, Saccharopolyspora erythraea