Quantification of actively transcribing RNA polymerase B molecules in human sperm chromatin and the effect of prostaglandin E1.
Run-on transcription experiments with sonicated chromatin from spermatozoa of healthy men revealed the presence of in vitro transcription. The number of actively transcribing RNA polymerase B molecules in sperm chromatin in vitro, isolated from healthy individuals, and the effect of prostaglandin El, were investigated. The results indicate that the number of RNA polymerase B molecules actively engaged in transcription increased one and a half times when 5 units of prostaglandin E1 was added to the reaction mixtures. These results correlate well with the authors' previous findings that the incorporation of labelled uridine triphosphate into pre-mRNA was higher when prostaglandin E1 was added to the reaction mixtures in run-on transcription experiments, and when sperm chromatin from healthy individuals was used as a template.
Pironcheva
G L
GL
Institute of Molecular Biology, Bulgarian Academy of Science, Sofia, Bulgaria.
Miteva
K D
KD
Zlatarev
S T
ST
Russev
G C
GC
D000527:Alprostadil / Q000494:pharmacology*; D002474:Cell-Free System; D002843:Chromatin / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D007248:Infertility, Male; D008297:Male; D012319:RNA Polymerase II / Q000378:metabolism*; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*
D002843:Chromatin; D012319:RNA Polymerase II; D000527:Alprostadil