Isolation and characterization of a novel epithelium-specific transcription factor, ESE-1, a member of the ets family.
We report here the isolation of a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, ESE-1 (for epithelium-specific Ets), which has features distinct from those of any other ets-related factor. ESE-1 contains two putative DNA binding domains: an ETS domain, which is unique in that the 5' half shows relatively weak homology to known ets factors, and an A/T hook domain, found in HMG proteins and various other nuclear factors. In contrast to any known ets factors, ESE-1 is expressed exclusively in epithelial cells. ESE-1 expression is induced during terminal differentiation of the epidermis and in a primary human keratinocyte differentiation system. The keratinocyte terminal differentiation marker gene, SPRR2A, is a putative target for ESE-1, since SPRR2A expression during keratinocyte differentiation correlates with induction of ESE-1 expression, and ESE-1 binds with high affinity to and transactivates the ets binding site in the SPRR2A promoter. ESE-1 also binds to and transactivates the enhancer of the Endo A gene, a potential target for ESE-1 in simple epithelia. Due to the important role that other ets factors play in cellular differentiation, ESE-1 is expected to be a critical regulator of epithelial cell differentiation.
Oettgen
P
P
Division of Immunology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
Alani
R M
RM
Barcinski
M A
MA
Brown
L
L
Akbarali
Y
Y
Boltax
J
J
Kunsch
C
C
Munger
K
K
Libermann
T A
TA
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D055233:Cornified Envelope Proline-Rich Proteins; D004268:DNA-Binding Proteins / Q000235:genetics; D000078404:Epidermal Cells; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental; D006609:High Mobility Group Proteins / Q000235:genetics; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry* / Q000166:cytology; D007633:Keratins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D009928:Organ Specificity; D011498:Protein Precursors / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics
D055233:Cornified Envelope Proline-Rich Proteins; D004268:DNA-Binding Proteins; C512897:ELF1 protein, human; C105529:ELF3 protein, human; C512896:Elf1 protein, mouse; D006609:High Mobility Group Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D011498:Protein Precursors; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; C101744:SPRR2A protein, human; C491091:Sprr2a1 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; D007633:Keratins