title: Oxygenation capacity of hypotonized and crosslinked rat erythrocytes.
abstract: Rat erythrocytes subjected to hypotonic-isotonic dialysis, or crosslinking with bifunctional reagents (glutaraldehyde and dimethyl suberimidate hydrochloride) show a high percentage of methemoglobin and decreased oxyhemoglobin content which implies a low oxygen carrying capacity. Such modified cells maintain reversible oxygen binding properties although, they present a high hemoglobin oxygen affinity (low P50) and a diminished cooperativity in binding oxygen to hemoglobin (low n). These results suggest a reduced capacity of liberating oxygen to tissues under low PO2. Changes produced in erythrocytes can not be restored even in the presence of energy (ATP), reduced glutathione and 2,3-bisphosphoglyceric acid during the dialysis process or after crosslinking/permeabilizing treatment.
authors.affiliation: Departamento de Bioquímica y Biología Molecular. Universidad de Alcalá. Campus Universitario, Madrid, Spain.
authors.forename: A I
authors.initials: AI
authors.lastname: García-Pérez
authors.forename: M T
authors.initials: MT
authors.lastname: Pérez
authors.forename: L
authors.initials: L
authors.lastname: Lucas
authors.forename: M
authors.initials: M
authors.lastname: Pinilla
authors.forename: J
authors.initials: J
authors.lastname: Luque
authors.forename: P
authors.initials: P
authors.lastname: Sancho
mesh_terms: D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D003432:Cross-Linking Reagents; D003956:Dialysis; D004120:Dimethyl Suberimidate; D004163:Diphosphoglyceric Acids / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism*; D005976:Glutaral; D005978:Glutathione / Q000494:pharmacology; D006447:Hemoglobin H / Q000378:metabolism; D007038:Hypotonic Solutions; D008297:Male; D008706:Methemoglobin / Q000378:metabolism; D009996:Osmotic Fragility; D010100:Oxygen / Q000097:blood*; D010108:Oxyhemoglobins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003432:Cross-Linking Reagents; D004163:Diphosphoglyceric Acids; D007038:Hypotonic Solutions; D010108:Oxyhemoglobins; D019794:2,3-Diphosphoglycerate; D004120:Dimethyl Suberimidate; D000255:Adenosine Triphosphate; D008706:Methemoglobin; D006447:Hemoglobin H; D005978:Glutathione; D010100:Oxygen; D005976:Glutaral