title: Melatonin protects bovine cerebral endothelial cells from hyperoxia-induced DNA damage and death.
abstract: Hyperoxia leads to excessive formation of reactive oxygen species (ROS). ROS cause damage to many cellular components, including DNA. Exposure of bovine cerebral endothelial cells to 95 or 100% oxygen resulted in an increase in DNA fragmentation, the appearance of DNA ladders, and cell death with morphological features suggestive of apoptosis. Melatonin, an antioxidant, reduced hyperoxia-induced DNA fragmentation and cell death in a dose-dependent manner. Results from the present study support the contention that ROS play a major role in DNA damage and apoptotic death. Melatonin is an effective agent in reducing ROS-mediated DNA fragmentation and death in bovine cerebral endothelial cells.
authors.affiliation: Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: A Y
authors.initials: AY
authors.lastname: Shaikh
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: L
authors.initials: L
authors.lastname: He
authors.forename: C Y
authors.initials: CY
authors.lastname: Hsu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004249:DNA Damage / Q000187:drug effects*; D004727:Endothelium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D018496:Hyperoxia / Q000188:drug therapy*; D008550:Melatonin / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010100:Oxygen / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D018696:Neuroprotective Agents; D008550:Melatonin; D010100:Oxygen