125I-labelling of an internally 75Se-labelled monoclonal antibody--biodistribution in tumour-bearing nude mice.
A monoclonal antibody, C215, was first internally labelled with 75Se-methionine and then labelled with 125I. The biodistribution of the dual-labelled [125I][75Se]C215 was studied in tumour-bearing nude mice killed 3 days after injection. The biodistribution of the dual-labelled [125I][75Se]C215 was compared with the biodistribution of single-labelled [131I]C215 and [75Se]C215. Iodine-labelled antibodies seem to be damaged during iodination, affecting the disappearance rate and tumour uptake. There were no signs of dehalogenation of circulating antibodies or antibodies taken up in the tumour.
Grétarsdóttir
J
J
Department of Radiation Physics, University of Göteborg, Sahlgrenska Hospital, Sweden.
Forssell Aronsson
E
E
Jacobsson
L
L
Hafsteinsdóttir
O
O
Holmberg
S
S
Hafström
L
L
Lindegren
S
S
Karlsson
B
B
Lindholm
L
L
Mattsson
S
S
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000493:pharmacokinetics*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012644:Selenium Radioisotopes / Q000737:chemistry*; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D012644:Selenium Radioisotopes