Molecular epidemiology of Vibrio cholerae O1 isolates from Colombia.
A total of 173 Vibrio cholerae O1 isolates from the recent cholera epidemic in Colombia was analysed by the polymerase chain reaction (PCR) for the genes encoding the A subunit of cholera toxin (ctxA) and the zonula occludens toxin (zot), and by ribotyping. All isolates were positive for ctx A and zot, which was confirmed by hybridisation. Ribotyping with restriction endonuclease Bg/I digestion of total DNA revealed three ribotypes: B5a comprising 165 (96.4%) isolates, and two new designated ribotypes B20 and B21a in six (3.5%) isolates and two (1.1%) isolates, respectively. These findings have significant public health implications.
Tamayo
M
M
Instituto Nacional de Salud, Santafé de Bogota, Colombia.
Koblavi
S
S
Grimont
F
F
Castañeda
E
E
Grimont
P A
PA
D015373:Bacterial Typing Techniques; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology; D002772:Cholera Toxin / Q000235:genetics*; D003105:Colombia / Q000453:epidemiology; D004196:Disease Outbreaks; D004731:Endotoxins; D005798:Genes, Bacterial; D006801:Humans; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012407:rRNA Operon
D004731:Endotoxins; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; C077728:zonula occludens toxin, Vibrio cholerae; D002772:Cholera Toxin