Partial inhibition of the in vitro infection of adult mouse dorsal root ganglion neurons by rabies virus using nicotinic antagonists.
The infection of target cells by rabies virus is effected through membrane receptors. Several authors have suggested that nicotinic receptors could be used by this virus, but no direct experimental evidence is available. In this study mouse dorsal root ganglia cells were treated with various nicotinic antagonists (dihydro-beta-eritroidine, mecamilamine, d-tubocurarin, hexametonium, alpha-bungarotoxin and erabutoxin). After incubation, the cultures were infected with rabies virus. Cells were fixed, and processed for immunodetection of rabies virus. Treatment with mecamilamine or d-tubocurarine reduced the percentage of infected neurons. None of the antagonists tested changed the percentage of infected non-neuronal cells.
Castellanos
J E
JE
Laboratorio de Neurociencias, Instituto Nacional de Salud, Santafé de Bogotá, Columbia. ins2@col1.telecom.com.co
Castañeda
D R
DR
Velandia
A E
AE
Hurtado
H
H
D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology; D002478:Cells, Cultured; D004870:Erabutoxins / Q000494:pharmacology; D005347:Fibroblasts / Q000821:virology; D005727:Ganglia, Spinal / Q000166:cytology / Q000821:virology; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000737:chemistry / Q000821:virology*; D018733:Nicotinic Antagonists / Q000494:pharmacology*; D011818:Rabies / Q000188:drug therapy*; D011978:Receptors, Nicotinic / Q000502:physiology*; D012583:Schwann Cells / Q000821:virology
D002038:Bungarotoxins; D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic; D004870:Erabutoxins