Research note: unsuitability of the MRS medium for the screening of hydrogen peroxide-producing lactic acid bacteria.
The effect of MRS broth on the stability of hydrogen peroxide (H2O2) has been studied. Known concentrations (1-100 micrograms ml-1) of H2O2 were prepared in distilled water, phosphate buffer (pH 7.0) and MRS broth (pH 6.2 and 3.9). H2O2 was very stable in aqueous and buffer solutions but it was rapidly degraded in MRS broth (pH 3.9). The presence of H2O2 in MRS broth (pH 6.2) could not be detected.
Rodríguez
J M
JM
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain. jmrodrig@eucmax.sim.ucm.es
Martínez
M I
MI
Suárez
A M
AM
Martínez
J M
JM
Hernández
P E
PE
D003470:Culture Media; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism*
D003470:Culture Media; D006861:Hydrogen Peroxide