Polymorphonuclear leukocyte lysosomal proteases, cathepsins B and D affect the fibrinolytic system in human umbilical vein endothelial cells.
To clarify the physiological role played by neutrophil lysosomal protease in cultured human umbilical vein endothelial cells (HUVEC), we studied the effects of cathepsins B and D released from activated polymorphonuclear leukocytes on the fibrinolytic system in HUVEC. Cathepsins B and D reduced the antigens of tissue-type plasminogen activator, and they increased both the antigens and the activity of plasminogen activator inhibitor-1. These results suggest that cathepsins B and D are involved in the thrombotic tendency, since they inhibited the fibrinolytic system in cultured HUVEC.
Kimura
Y
Y
New Drug Research Department, High Quality-Life Research Laboratories, Sumitomo Metal Industries, Souraku-gun, Kyoto, Japan.
Yokoi-Hayashi
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K
D002401:Cathepsin B / Q000494:pharmacology / Q000502:physiology*; D002402:Cathepsin D / Q000494:pharmacology / Q000502:physiology*; D002478:Cells, Cultured / Q000187:drug effects; D004730:Endothelium, Vascular / Q000502:physiology*; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology*; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis; D010959:Tissue Plasminogen Activator / Q000032:analysis
D017395:Plasminogen Activator Inhibitor 1; D010959:Tissue Plasminogen Activator; D002401:Cathepsin B; D002402:Cathepsin D