Loss of high-molecular-weight proteins in liver microsomes of rats treated with hepatocarcinogens.
Microsomal preparations from rats treated with 2-acetylaminofluorene (2-AAF) or with 4-dimethylaminoazobenzene (4-DAB), alone or followed by phenobarbital (PB), showed almost complete loss of microsomal proteins of molecular weights higher than 60,000, as shown by Sephadex G-150 gel filtration technique. Induction of a number of microsomal proteins in the lower ranges of molecular weights was also recorded due to the treatment of these two hepatocarcinogens with and without the use of PB as a promoter. These modifications in the protein patterns of microsomes might be due to altered genetic expression resulting in uncontrolled cell division/cell cycle.
Khongwir
B S
BS
Department of Biochemistry, Panjab University, Chandigarh, India.
Dani
H M
HM
Dhanda
J S
JS
D015073:2-Acetylaminofluorene / Q000008:administration & dosage / Q000633:toxicity; D000284:Administration, Oral; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D004124:p-Dimethylaminoazobenzene / Q000008:administration & dosage / Q000633:toxicity
D002273:Carcinogens; D011506:Proteins; D015073:2-Acetylaminofluorene; D004124:p-Dimethylaminoazobenzene