Prolonged intestinal afferent nerve discharge in response to cholecystokinin-58 compared to cholecystokinin-8 in rats.
Intestinal afferents are sensitive to cholecystokinin (CCK) octapeptide. However, CCK-58 may be a more biologically relevant molecule. Therefore, recordings from jejunal mesenteric afferent bundles were secured for extracellular multi-unit recording and the responses to CCK-8 and CCK-58 compared. CCK-8 and CCK-58 (i.v.) evoked a dose-dependent, devazepide sensitive, increase in afferent nerve discharge. Peak discharge frequency was higher for CCK-8 at all doses (P < 0.05). However, response duration was more prolonged for CCK-58 (P < 0.05) at 200 pmol.kg(-1). This resulted in an overall increase in area under the curve of CCK-58 compared to CCK-8 (P < 0.05). CCK-58 stimulates afferent discharge in a different pattern than CCK-8 and, therefore, may have differential biological effects.
Kreis
M E
ME
University Hospital, Department of General Surgery, Tübingen, Germany.
Zittel
T T
TT
Raybould
H E
HE
Reeve
J R
JR
Grundy
D
D
D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D002766:Cholecystokinin / Q000494:pharmacology*; D020109:Devazepide; D005071:Evoked Potentials / Q000187:drug effects*; D007583:Jejunum / Q000294:innervation*; D008297:Male; D009130:Muscle, Smooth / Q000294:innervation; D051381:Rats; D011919:Rats, Inbred Strains; D012844:Sincalide / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D013153:Splanchnic Nerves / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D001570:Benzodiazepinones; C038823:cholecystokinin 58; D002766:Cholecystokinin; D020109:Devazepide; D012844:Sincalide