No influence of a single bout of exercise on urinary excretion of 8-hydroxy-deoxyguanosine in humans.
We investigated the effect of a single bout of intensive exercise on the excretion of 8-hydroxy-deoxyguanosine in the 24 h urine from healthy non-smokers. We studied three exercise tests in Experiment 1; which consisted of incremental exercise to exhaustion on a treadmill in eleven male long distance runners. Experiment 2; which comprised incremental exercise until reaching exhaustion on a bicycle ergometer in six male untrained subjects. Experiment 3; which consisted of a 20 km run by eleven male long distance runners. No differences in the urinary 8-hydroxy-deoxyguanosine excretion were observed from days 1 to 3 after each respective exercise regimen. However, significant increases in the plasma creatine kinase activity were observed at 24 h or 48 h after exercise, except for Experiment 2. Our results thus suggest that the oxidative stress during a single bout of intensive exercise does not result in an accumulation of oxidative DNA damage.
Sumida
S
S
Department of Health and Sports Science, Osaka Gakuin University, Japan. sumida@center.osaka-gu.ac.jp
Okamura
K
K
Doi
T
T
Sakurai
M
M
Yoshioka
Y
Y
Sugawa-Katayama
Y
Y
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D000328:Adult; D003402:Creatine Kinase / Q000097:blood; D003404:Creatinine / Q000652:urine; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005080:Exercise Test; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D005082:Physical Exertion / Q000502:physiology*; D010809:Physical Fitness; D012420:Running
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003402:Creatine Kinase; D003849:Deoxyguanosine