title: A flagellar-specific ATPase (FliI) is necessary for flagellar export in Helicobacter pylori.
abstract: Although flagellar motility is essential for the colonisation of the stomach by Helicobacter pylori, little is known about the regulation of flagellar biosynthesis in this organism. We have identified a gene in H. pylori, designated fliI, whose deduced amino acid sequence revealed extensive homology with the FliI/LcrB/InvC family of proteins which energise the export of flagellar and other virulence factors in several bacterial species. An isogenic mutant of fliI was non-motile and synthesised reduced amounts of flagellin and hook protein subunits. The majority (> 99%) of mutant cells were completely aflagellate. These results suggest that FliI is a novel ATPase involved in flagellar export in H. pylori.
authors.affiliation: Department of Medical Microbiology, St. Bartholomew's Hospital, West Smithfield, London, UK.
authors.forename: P J
authors.initials: PJ
authors.lastname: Jenks
authors.forename: S
authors.initials: S
authors.lastname: Foynes
authors.forename: S J
authors.initials: SJ
authors.lastname: Ward
authors.forename: C
authors.initials: C
authors.lastname: Constantinidou
authors.forename: C W
authors.initials: CW
authors.lastname: Penn
authors.forename: B W
authors.initials: BW
authors.lastname: Wren
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005407:Flagella / Q000378:metabolism*; D005408:Flagellin / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009154:Mutation; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D006180:Proton-Translocating ATPases; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; C097681:FlgE protein, Bacteria; D011506:Proteins; D005408:Flagellin; C067138:flaA protein, bacteria; C069251:fliI protein, bacteria; C416532:flaB flagellin; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases