Characterization of peptides that bind the tumor-associated Thomsen-Friedenreich antigen selected from bacteriophage display libraries.
Peptides with high affinities and specificities for numerous proteins and nucleic acids have been previously identified from random peptide bacteriophage display libraries. Here, random peptide bacteriophage display libraries were used to identify sequences that bound the cancer-associated Thomsen-Friedenreich glycoantigen (T antigen). The T antigen, present on most malignant cells, contains an immunodominant Gal beta1 --> 3GalNAc alpha disaccharide unmasked on the surfaces of most carcinomas. This antigen has been postulated to be involved in tumor cell aggregation and metastasis. Two 15 amino acid random peptide bacteriophage display libraries were affinity selected with glycoproteins displaying T antigen on their surfaces. Sequence analysis revealed that many of the peptides shared homology with sugar recognition sites in several carbohydrate-binding proteins. A comparison of affinity selected sequences from both libraries yielded a common motif (W-Y-A-W/F-S-P) rich in aromatic amino acids. Four peptides, corresponding to the affinity selected sequences, were chemically synthesized and characterized for their carbohydrate recognition properties. The synthetic peptides exhibited high specificities and affinities to T antigen displayed on asialofetuin or conjugated to bovine serum albumin (Kd = 5 nM for MAP-P30 binding to asialofetuin) as well as free T-antigen disaccharide in solution (Kd = 10 microM for MAP-P30, 20 microM for P10). Two peptides, P30 and P10, demonstrated high affinities and specificities for both asialofetuin and T antigen in solution. Iodination of a lone tyrosine residue in each sequence dramatically reduced their abilities to bind T antigen, suggesting that the tyrosine residue plays an important role in carbohydrate recognition. That these peptides are of functional significance is evidenced by the ability of both P30 and P10 to inhibit asialofetuin-mediated melanoma cell aggregation in vitro and to compete with peanut lectin for binding to T antigen displayed on the surface of MDA-MB-435 breast carcinoma cells in situ.
Peletskaya
E N
EN
Department of Biochemistry, University of Missouri, Columbia 65211, USA.
Glinsky
V V
VV
Glinsky
G V
GV
Deutscher
S L
SL
Quinn
T P
TP
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D001212:Asialoglycoproteins / Q000378:metabolism; D001667:Binding, Competitive; D001943:Breast Neoplasms / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism; D002449:Cell Aggregation; D060748:Fetuins; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008545:Melanoma / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D019887:Peanut Agglutinin; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D012710:Serum Albumin, Bovine; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D001212:Asialoglycoproteins; D060748:Fetuins; D037102:Lectins; D019887:Peanut Agglutinin; D019151:Peptide Library; D010455:Peptides; D000509:alpha-Fetoproteins; C017029:asialofetuin; D012710:Serum Albumin, Bovine; C016467:Thomsen-Friedenreich antigen; D014443:Tyrosine