[Breath condensate as a method of noninvasive assessment of inflammation mediators from the lower airways].
The detection of mediators from the lower airways still depends on invasive or provoking sampling techniques like bronchoalveolar lavage (BAL) or induced sputum, respectively. Both methods affect the specimen itself. In contrast, the breathing condensate opens the possibility to get native specimens from lower airways during breathing at rest. The breathing condensate was obtained by freezing of exhaled air. The equipment was developed in the FILT Res. Soc. Ltd.. The method is applied for a patent. Leukotriene B4 and Leukotriene C4D4E4F4 were measured in the exhalation of asthmatics, patients with different airway disorders and healthy volunteers. In an additional study the condensate was obtained before and after of a non-specific bronchial challenge test. In asthmatics a close correlation between leukotriene concentration of the condensate and the degree of asthmatic disease according to "International Consensus Report" was found, but no correlation to lung function tests. Within a bronchial challenge test applying histamine the release of leukotrienes was shown to be more sensitive to the challenge test than a lung function test. The results of the study indicate new diagnostic possibilities in lung diseases using the detection of non volatile substances in the exhaled air.
Becher
G
G
FILT Forschungsgesellschaft für Lungen- und Thorax-erkrankungen mbH, Berlin.
Winsel
K
K
Beck
E
E
Neubauer
G
G
Stresemann
E
E
D001249:Asthma / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests / Q000379:methods*; D001985:Bronchial Provocation Tests; D006632:Histamine; D006801:Humans; D015289:Leukotrienes / Q000032:analysis*; D012140:Respiratory Tract Diseases / Q000378:metabolism*
D015415:Biomarkers; D015289:Leukotrienes; D006632:Histamine
Das Atemkondensat als Methode zur nicht-invasiven Erfassung von Entzündungsmediatoren aus den unteren Atemwegen.