Expression, exon-intron organization, and chromosome mapping of the human sodium iodide symporter.
The active iodide uptake of the thyroid gland in humans is mediated by the human sodium iodide symporter (hNIS). In this report, we show that hNIS expression was detected primarily in thyroid tissue, but also in breast, colon, and ovary tissues. Expression of hNIS is greatly reduced in thyroid tumors compared to normal thyroid tissue. Among tumor tissues, hNIS expression appears to be variable, consistent with the variable response to radioiodide treatment observed for thyroid carcinomas. The coding region of hNIS is interrupted by 14 introns, and the nucleotide sequence of each exon-intron junction is reported. Using this information, an alternatively spliced form of hNIS was identified. Finally, the chromosome location of the hNIS gene was mapped to chromosome 19p.
Smanik
P A
PA
Department of Physiology, The Ohio State University, Columbus 43210, USA.
Ryu
K Y
KY
Theil
K S
KS
Mazzaferri
E L
EL
Jhiang
S M
SM
D001483:Base Sequence; D001692:Biological Transport / Q000502:physiology; D015152:Blotting, Northern; D001940:Breast / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D003106:Colon / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D005091:Exons; D005260:Female; D005784:Gene Amplification; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns; D007454:Iodides / Q000378:metabolism; D017136:Ion Transport; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D010053:Ovary / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D016133:Polymerase Chain Reaction; D012964:Sodium / Q000378:metabolism; D027981:Symporters; D013961:Thyroid Gland / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D013964:Thyroid Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000503:physiopathology; D000099209:Sodium-Iodide Symporters
D002352:Carrier Proteins; D007454:Iodides; D008565:Membrane Proteins; D012964:Sodium; D027981:Symporters; D000099209:Sodium-Iodide Symporters