Immunological identification of the alternative oxidase of Acanthamoeba castellanii mitochondria.
Mitochondria of the protozoa Acanthamoeba castellanii possess a cyanide-insensitive oxidase cross-reacting with monoclonal antibodies raised against the plant alternative oxidase. Immunoblotting revealed three monomeric forms (38, 35, and 32 kDa) and very low amounts of a single 65 kDa dimeric form. Cross-linking studies suggest that while in plants the alternative oxidase occurs as a dimer, in amoeba it functions as a monomer. Immunologically detectable protein levels change with the age of amoeba cell culture. Increased amounts of the 35 kDa protein are accompanied by an increase in the activity of cyanide-resistant respiration.
Jarmuszkiewicz
W
W
Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Poznan University, Poland. wiesiaj@vm.amu.edu.pl
Wagner
A M
AM
Wagner
M J
MJ
Hryniewiecka
L
L
D000048:Acanthamoeba / Q000201:enzymology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003958:Diamide / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D015151:Immunoblotting; D008301:Maleimides / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D024101:Mitochondrial Proteins; D010088:Oxidoreductases / Q000032:analysis*; D010940:Plant Proteins; D000099188:Alternative Oxidase
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D003958:Diamide; D004229:Dithiothreitol; D008301:Maleimides; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D000099188:Alternative Oxidase; C025285:N-(4-carboxycyclohexylmethyl)maleimide N-hydroxysuccinimide ester