Detection of Aequorea victoria green fluorescent protein by capillary electrophoresis laser induced fluorescence detection.
Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post-capillary laser-induced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0 x 10(-12) M protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules.
Craig
D B
DB
Department of Chemistry, University of Alberta, Edmonton, Canada.
Wong
J C
JC
Dovichi
N J
NJ
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D049452:Green Fluorescent Proteins; D007834:Lasers; D008164:Luminescent Proteins / Q000032:analysis*; D037401:Scyphozoa / Q000737:chemistry
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins