Disaccharide analysis of skin glycosaminoglycan in localized scleroderma.
BACKGROUND
Altered metabolism of skin glycosaminoglycan has rarely been investigated in localized scleroderma in contrast to systemic sclerosis.


OBJECTIVE
The aim of this investigation is to elucidate the change in skin glycosaminoglycan of localized scleroderma.


METHODS
We analyzed 5 skin samples of localized scleroderma and 10 site-matched control skin samples using high performance liquid chromatography after 1-phenyl-3-methyl-5-pyrasolone labeling.


RESULTS
The involved skin constantly showed an increased amount of delta Di-4S(DS), the main disaccharide unit of dermatan sulfate, a decreased amount of delta Di-HA, the main disaccharide unit of hyaluronic acid, and an elevated ratio of delta Di-4S(DS)/delta Di-HA as compared with the uninvolved skin or the site matched control skin.


CONCLUSIONS
These results correlate well with those findings in systemic sclerosis, indicating that the alteration in skin GAG may be closely related to the fibrotic process.
Yokoyama
Y
Y
Department of Dermatology, Gunma University School of Medicine, Maebashi, Japan.
Ishikawa
O
O
Miyachi
Y
Y
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001707:Biopsy, Needle; D046508:Culture Techniques; D004187:Disaccharides / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012594:Scleroderma, Localized / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000378:metabolism / Q000473:pathology
D004187:Disaccharides; D006025:Glycosaminoglycans