NKRP1A molecule is involved in transendothelial migration of CD4+ human T lymphocytes.
Among human CD4+ T lymphocytes, 5-20% express the C-type lectin molecule NKRP1A. Interestingly, CD4+ NKRP1A+ T lymphocytes express high levels of beta 1 and beta 2 integrins, thus representing a T lymphocyte subset that can possibly adhere and migrate through vascular endothelium. Indeed, resting CD4+ NKRP1A+ lymphocytes, differently from the CD4+ NKRP1A- subset, migrated across endothelial cell monolayers in a Transwell chamber system. This transendothelial migration was strongly reduced after pre-treatment with an anti-NKRP1A monoclonal antibody (mAb). In addition, the NKRP1A negative Jurkatt CD4+ T-cell line that had been stably transfected with NKRP1A cDNA, migrated more rapidly and efficiently than untransfected Jurkatt cells. Finally, mAb-mediated cross-linking of NKRP1A molecule in CD4+ T lymphocytes induced the upregulation of the LFA1 Mg2+ binding site as well as beta 1 and beta 2 integrin chains. Altogether, these findings indicate that NKRP1A molecule is involved in transendothelial migration of resting CD4+ T lymphocytes.
Poggi
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Istituto Nazionale per la Ricerca sul Cancro e Centro di Biotecnologie Avanzate, Genova, Italy.
Costa
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Zocchi
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Moretta
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D000954:Antigens, Surface / Q000235:genetics / Q000502:physiology*; D018821:CD18 Antigens / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D019012:Integrin beta1 / Q000096:biosynthesis; D019169:Jurkat Cells; D037102:Lectins / Q000235:genetics / Q000502:physiology*; D037181:Lectins, C-Type; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D055651:NK Cell Lectin-Like Receptor Subfamily B
D000954:Antigens, Surface; D018821:CD18 Antigens; D019012:Integrin beta1; C527203:KLRB1 protein, human; D037102:Lectins; D037181:Lectins, C-Type; D016169:Lymphocyte Function-Associated Antigen-1; D055651:NK Cell Lectin-Like Receptor Subfamily B