Quantification of IgG on erythrocytes of patients and normals by a radio-ligand-binding assay.
A monoclonal IgG anti-human IgG, 1B12, was used in a radio-ligand-binding assay to quantify IgG on erythrocytes of patients and normals. The assay detected a range of 10-700 IgG molecules. Good correlation was achieved between the number of molecules and the strength of agglutination in antiglobulin tests performed in capillary tubes. The assay was capable of detecting subagglutinating immune bound IgG on erythrocytes from patients with systemic lupus erythematosus (SLE).
Giles
C M
CM
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Davies
K A
KA
Loizou
S
S
Moulds
J J
JJ
Walport
M J
MJ
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D017153:Antibodies, Anticardiolipin / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007202:Indicators and Reagents; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D011869:Radioligand Assay
D000888:Antibodies, Anti-Idiotypic; D017153:Antibodies, Anticardiolipin; D000911:Antibodies, Monoclonal; D003176:Complement C3; D003181:Complement C4; D007074:Immunoglobulin G; D007202:Indicators and Reagents