Modular structures in the mushroom body of the cockroach.
The mushroom body (MB) is a higher center of the insect brain and is critical to olfactory and other forms of associative memory. Here, we report that repetitive modular subunits, which we refer to as slabs, are present in the internal matrix of the alpha lobe, a major output neuropil of the MB in the cockroach. The methods employed were osmium-ethyl gallate, Bodian-reduced silver, and Golgi staining procedures. A total of 15 dark and 15 pale slabs, each consisting of specific subsets of intrinsic neurons (Kenyon cells), alternate throughout the length of the alpha lobe. One of the major classes of MB output neurons, which are postsynaptic to Kenyon cells, exhibited segmented dendritic arbors that interact with every other slabs, i.e. only either dark or pale slabs. As each output neuron interacts with each specific set of dark or pale slabs, the slab likely functions as a unit for transmitting MB output signals.
Mizunami
M
M
Laboratory of Neuro-Cybernetics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Japan. makoto@bmcd.hokudai.ac jp
Iwasaki
M
M
Nishikawa
M
M
Okada
R
R
D000818:Animals; D001244:Association; D001921:Brain / Q000166:cytology / Q000502:physiology; D003058:Cockroaches / Q000502:physiology*; D005260:Female; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000502:physiology; D008297:Male; D008568:Memory / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D016622:Silver Staining