High-sensitivity mass spectrometry for analysis of posttranslational modifications.
Pulsed fast atom bombardment ionization (pulsed-FAB) mass spectrometry has been developed to improve the sensitivity of tandem mass spectrometry (MS/MS), allowing it to be used for the analysis of very small samples. MS/MS, when used with a magnetic four-sector instrument coupled with the pulsed-FAB system, allows significant enhancement in product ion intensity of over ten-fold in magnitude over conventional FAB. MS/MS was applied to the structural analysis of a unique nuclear protein, designated p28, which was isolated from a histone fraction obtained from starfish testes. The results clearly show that protein p28 is a heterodimer composed of testicular histones H2B and H4 which are cross-linked between Gln9 of H2B and Lys5 of H4.
Takao
T
T
Institute for Protein Research, Osaka University, Japan. tak@protein.osaka.u.ac.jp
Shimizu
T
T
Ikegami
S
S
Shimonishi
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D006657:Histones / Q000032:analysis* / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D012680:Sensitivity and Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*; D013215:Starfish; D013373:Substance P / Q000032:analysis / Q000378:metabolism; D013737:Testis / Q000737:chemistry / Q000378:metabolism
D006657:Histones; D013373:Substance P