Characterization of a recombinant fragment that contains a carbohydrate recognition domain of the filamentous hemagglutinin.
The filamentous hemagglutinin (FHA) of Bordetella pertussis plays an important role in establishing infection by attaching the bacteria to the ciliated respiratory epithelial cells. Expression of DNA encoding residues 1141 to 1279 of FHA in Escherichia coli yields a protein of 18,000 Da that exhibits some of the carbohydrate recognition properties of FHA (S. M. Prasad, Y. Yin, E. Rodzinski, E. I. Tuomanen, and H. R. Masure, Infect. Immun. 61:2780-2785, 1993). We have constructed an E. coli strain that expresses this protein, designated fragment A, in a soluble form at markedly elevated levels. Fragment A could be purified with high purity and yields and was immunogenic in mice. Both fragment A and anti-fragment A sera inhibited the binding of B. pertussis to asialo-GM2 and to rabbit ciliated cells. These observations demonstrate that this fragment of FHA contains a cellular binding domain capable of eliciting functional antibodies.
Liu
D F
DF
Wyeth Lederle Vaccines and Pediatrics, West Henrietta, New York 14586, USA. Dai Fang_Liu@internetmail.pr.cyanamid.com
Phillips
E
E
Wizemann
T M
TM
Siegel
M M
MM
Tabei
K
K
Cowell
J L
JL
Tuomanen
E
E
D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D001422:Bacterial Adhesion; D001665:Binding Sites; D001886:Bordetella pertussis / Q000276:immunology*; D050260:Carbohydrate Metabolism; D004926:Escherichia coli / Q000235:genetics; D006388:Hemagglutinins / Q000276:immunology*; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology*; D010567:Pertussis Vaccine / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D010566:Virulence Factors, Bordetella
D018829:Adhesins, Bacterial; D006388:Hemagglutinins; D010446:Peptide Fragments; D010567:Pertussis Vaccine; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis