Analysis of non-polar deletion mutations in the genes of the spo0K (opp) operon of Bacillus subtilis.
The spo0K (opp) operon of Bacillus subtilis encodes an oligopeptide permease that is required for uptake of oligopeptides, development of genetic competence, and initiation of sporulation. We made in-frame, non-polar deletion mutations in each of the first four genes of the five-gene spo0K operon and tested effects on oligopeptide transport, sporulation, and expression of competence genes. spo0KA, B, C, and D were required for sporulation, competence development, and oligopeptide transport. Disruption of spo0KE caused a less severe phenotype than did disruption of any of the other genes of the operon.
LeDeaux
J R
JR
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Solomon
J M
JM
Grossman
A D
AD
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D001426:Bacterial Proteins; D001692:Biological Transport; D005798:Genes, Bacterial / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D009842:Oligopeptides / Q000378:metabolism; D009876:Operon / Q000235:genetics; D010453:Peptide Synthases / Q000235:genetics; D017384:Sequence Deletion / Q000502:physiology*; D013171:Spores, Bacterial; D014169:Transformation, Bacterial / Q000235:genetics
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D009842:Oligopeptides; C037230:oligopeptide permease, Bacteria; D010453:Peptide Synthases; C069542:surfactin synthetase