Brain-derived neurotrophic factor induced stimulation of septal choline acetyltransferase activity in ethylcholine mustard aziridinium treated rats.
We evaluated whether brain-derived neurotrophic factor (BDNF) can stimulate choline acetyltransferase (ChAT) activity in the septo-hippocampal pathway of ethylcholine mustard aziridinium (AF64A) and non-AF64A-treated rats. Rats received either AF64A (1.5 nmol/ ventricle) or a sham (non-AF64A-treated) injection into the lateral ventricles. BDNF infusion (at a dose range of 0.1-171 microg) into either the lateral ventricle or hippocampus of AF64A-treated rats for 14 days increased septal ChAT activity (26% and 41%, respectively). BDNF did not reverse the decrease in hippocampal ChAT activity (20-60%) produced by AF64A. BDNF infusion did not change ChAT activity in the septo-hippocampal pathway of non-AF64A-treated rats. Thus, septo-hippocampal cholinergic neurons in AF64A-treated rats are sensitive to BDNF while those of non-AF64A-treated rats (normal) are not responsive to this neurotrophin.
Willson
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Loyola University Chicago Stritch School of Medicine, Department of Pharmacology and Experimental Therapeutics, Maywood, IL 60153, USA. Chris.A.willson@mbb.ki.se
Hanin
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D000818:Animals; D001388:Aziridines / Q000494:pharmacology*; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002794:Choline / Q000031:analogs & derivatives* / Q000494:pharmacology; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D007276:Injections, Intraventricular; D008297:Male; D008845:Microinjections; D009466:Neuromuscular Blocking Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012686:Septal Nuclei / Q000187:drug effects / Q000201:enzymology*; D014118:Toxins, Biological / Q000494:pharmacology
D001388:Aziridines; D019208:Brain-Derived Neurotrophic Factor; D009466:Neuromuscular Blocking Agents; D014118:Toxins, Biological; C044894:ethylcholine aziridinium; D002795:Choline O-Acetyltransferase; D002794:Choline