Early studies on DNA-based immunizations for measles virus.
DNA-mediated immunizations have been used to raise neutralizing antibodies for measles virus. Single inoculations of plasmids expressing measles hemagglutinin or fusion glycoproteins raised neutralizing antibody in BALB/c mice. Plasmids expressing the hemagglutinin glycoprotein (both normal and secreted) raised neutralizing responses that persisted for 1 year. For both forms of hemagglutinin, the effectiveness of the raised antibody (ratio of neutralizing activity to ELISA activity) was similar. High titers of neutralizing antibody were also raised by inoculation of rabbits with the hemagglutinin and fusion glycoprotein-expressing plasmids.
Yang
K
K
Department of Pathology, University of Massachusetts Medical School, Worcester 01655-0125, USA.
Mustafa
F
F
Valsamakis
A
A
Santoro
J C
JC
Griffin
D E
DE
Robinson
H L
HL
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D019470:Biolistics; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D008458:Measles Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D008459:Measles virus / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011817:Rabbits; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014709:Vero Cells
D000914:Antibodies, Viral; D004279:DNA, Viral; D008458:Measles Vaccine; D019444:Vaccines, DNA