title: Adenovirus-mediated wild-type p53 gene transfer and overexpression induces apoptosis of human glioma cells independent of endogenous p53 status.
abstract: Mutation or inactivation of the p53 tumor suppression gene is an early alteration in the transformation of glial cells to gliomas. To study the effect of exogenous wild-type p53 on glioma cell growth, human glioma lines U251 MG, U87 MG and A172 were infected with an adenovirus vector expressing either wild-type p53 or bacterial lacZ. Rapid cell death occurred only in the p53-transduced cell lines and was characterized by nuclear condensation, formation of nucleosomal DNA ladders, and positive in situ end-labeling of DNA, suggesting that apoptosis had been induced. The U87 MG cell line that contains wild-type p53 as evidenced by wild-type p53-dependent transcription activity also underwent apoptosis within 2 to 3 days after infection. These results suggest that the presence of endogenous wild-type p53 does not preclude apoptosis by overexpression of exogenous p53.
authors.affiliation: Department of Neurology & Neurosurgery, McGill University, Montreal, QC, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Lochmüller
authors.forename: V W
authors.initials: VW
authors.lastname: Yong
authors.forename: G
authors.initials: G
authors.lastname: Karpati
authors.forename: J
authors.initials: J
authors.lastname: Nalbantoglu
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D015870:Gene Expression; D018014:Gene Transfer Techniques; D016158:Genes, p53; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D051381:Rats; D014407:Tumor Cells, Cultured