Structure of the O18 antigen from Acinetobacter baumannii.
The polymeric O antigen was obtained from lipopolysaccharide extracted from isolated, defatted cell walls of the reference strain for Acinetobacter baumannii serogroup O18. Monosaccharide analyses and NMR spectra established that the polymer had a regular structure with a repeating unit based on residues of D-galactose (2), N-acetyl-D-galactosamine (1), and N-acetyl-D-mannosamine (1). Further interpretation of the NMR spectra, combined with the results of methylation analysis and a Smith degradation, showed that the repeating unit had the following structure. beta-D-ManpNAc-(1-->4)-alpha-D-Galp 1 decreases 4 -->3)-beta-D-GalpNAc-(1-->3)-beta-D-Galp-(1-->.
Haseley
S
S
School of Chemistry, University of Hull, UK.
Wilkinson
S G
SG
D000150:Acinetobacter / Q000276:immunology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D019081:O Antigens / Q000737:chemistry*; D017421:Sequence Analysis
D009005:Monosaccharides; D019081:O Antigens