title: Neurogenic inflammation in cholecystitis.
abstract: Neurogenic inflammation implies stimulation of nerves with resultant inflammation in tissue surrounding the nerve terminals. We hypothesized that neurogenic inflammation has a role in cholecystitis. Capsaicin (stimulant of afferent, nociceptive neurons), 6-hydroxydopamine (stimulates release of peptides from sympathetic nerve terminals), bradykinin, lipopolysaccharide, and saline were instilled into guinea pig gallbladders for 24 hr (N = 5 in each group). In parallel, test agents were instilled with 1% Iidocaine. Water transport across gallbladder mucosa, myeloperoxidase and interluekin-1 release from gallbladder tissue, and prostaglandin E2 in luminal fluid were measured. Capsaicin caused water secretion and significant release of myeloperoxidase, interleukin-1, and prostaglandin-E2, effects that were blocked by Iidocaine. 6-Hydroxydopamine did not affect water transport or prostaglandin E2, but did cause myeloperoxidase and interleukin-1 release. Bradykinin- and lipopolysaccharide-induced inflammation were partially inhibited by lidocaine. Taken together, these results suggest that neurogenic inflammation has a role in the pathophysiology of cholecystitis.
authors.affiliation: Department of Surgery, Northwestern University Medical School, Chicago, Illinois 50611, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Prystowsky
authors.forename: R V
authors.initials: RV
authors.lastname: Rege
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D002764:Cholecystitis / Q000209:etiology / Q000503:physiopathology*; D005704:Gallbladder / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism; D006168:Guinea Pigs; D008012:Lidocaine / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009411:Nerve Endings / Q000187:drug effects; D009412:Nerve Fibers / Q000187:drug effects; D009619:Nociceptors / Q000187:drug effects; D016627:Oxidopamine / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D016627:Oxidopamine; D008012:Lidocaine; D002211:Capsaicin; D001920:Bradykinin