Expression of human leukocyte antigens class I and class II on cultured biliary epithelial cells after cytomegalovirus infection.
The influence of cytomegalovirus (CMV) infection on the HLA expression on cultured biliary epithelial cells (BEC) was investigated. CMV-infection augmented expression of HLA class I but not of HLA class II. CMV reduced the IFN-gamma-mediated induction of the de novo expression of HLA class II while the stimulated expression of HLA class I was not impaired. Autologous but not allogeneic PBL responded to CMV-infected BEC. This response resulted in upregulation of HLA class I on BEC which was significantly higher compared with the expression on infected BEC alone or on uninfected BEC cocultured with autologous PBL. The results suggest that CMV modulates the immunogenic potential of BEC, which is important for the HLA and CMV-mediated pathomechanisms in vivo.
Scholz
M
M
Department of General Surgery, Johann Wolfgang Goethe University, Frankfurt am Main, Germany.
Cinatl
J
J
Blaheta
R A
RA
Kornhuber
B
B
Markus
B H
BH
Doerr
H W
HW
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology / Q000821:virology; D005704:Gallbladder / Q000166:cytology / Q000276:immunology / Q000821:virology; D006684:HLA-DR Antigens / Q000096:biosynthesis*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D006801:Humans
D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I