Shearing DNA for genomic library construction.
Methods and reagents is a unique monthly column that highlights current discussion in the newsgroup bionet.molibio.methds-reagnts, available on the internet. This month's column discusses the pros and cons of various techniques used to shear DNA for shotgun cloning. For details on how to partake in the newsgroup, see the accompanying box.
Hengen
P N
PN
National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA. pnh@ncifcrf.gov
D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D015723:Gene Library; D008863:Microspheres; D009330:Nebulizers and Vaporizers; D013010:Sonication
D004247:DNA