A role for Edman degradation in proteome studies.
Advances in protein database design and the software used to access the sequence data has led to progress in using protein attributes such as amino acid composition and peptide masses to identify proteins separated by two-dimensional electrophoresis. However, Edman degradation remains the principal technique for protein identification and it presents a significant bottleneck in the progress towards rapid protein identification. Simple modifications to the sequencing hardware, which automate the delivery of protein spots into the sequencer, and parallel sequencing of the protein spots represent a significant advance in the use of Edman degradation to rapidly generate the powerful protein attribute, an N-terminal sequence tag.
Gooley
A A
AA
Australian Proteome Analysis Facility and the Macquarie University Centre for Analytical Biotechnology, Sydney. andrew.gooley@mq.edu.au
Ou
K
K
Russell
J
J
Wilkins
M R
MR
Sanchez
J C
JC
Hochstrasser
D F
DF
Williams
K L
KL
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001798:Blood Proteins / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D005103:Expert Systems; D006801:Humans; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds; D017421:Sequence Analysis / Q000379:methods*
C043517:2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinene 2-oxide; D000596:Amino Acids; D001798:Blood Proteins; D009943:Organophosphorus Compounds