title: Antigen-induced interferon-gamma and interleukin-2 responses of cattle inoculated with Mycobacterium bovis.
abstract: Bovine purified protein derivative (PPD)-induced interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) mRNA expression was measured in peripheral blood lymphocyte cultures of cattle inoculated with Mycobacterium bovis and compared to cytokine protein levels as measured by IFN-gamma enzyme-linked immunosorbent assay and IL-2 bioassay. For individual animals, positive correlations were observed between mRNA and protein levels of bovine PPD-induced IFN-gamma and IL-2, although the correlations were stronger for IFN-gamma. Expression of these two cytokines also correlated with responses from a comparative intradermal test and a M. bovis antibody test. At 7 and 20 weeks after inoculation, bovine PPD-induced IFN-gamma and IL-2 mRNA expression was detected in all animals with tuberculous lesions and in a proportion of the M. bovis-inoculated animals with no lesions. Correlation of antigen-induced IFN-gamma and IL-2 with other immune parameters suggests that these two cytokines play an important role in the immune response to bovine tuberculosis.
authors.affiliation: AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
authors.forename: K H
authors.initials: KH
authors.lastname: Ng
authors.forename: F E
authors.initials: FE
authors.lastname: Aldwell
authors.forename: D N
authors.initials: DN
authors.lastname: Wedlock
authors.forename: J D
authors.initials: JD
authors.lastname: Watson
authors.forename: B M
authors.initials: BM
authors.lastname: Buddle
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000662:veterinary; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000032:analysis / Q000096:biosynthesis*; D007428:Intradermal Tests / Q000662:veterinary; D009163:Mycobacterium bovis / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014373:Tuberculin / Q000276:immunology*; D014380:Tuberculosis, Bovine / Q000276:immunology / Q000473:pathology
chemical_list: D007376:Interleukin-2; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014373:Tuberculin; D007371:Interferon-gamma