title: Evaluation of sugar cane hemicellulose hydrolyzate for cultivation of yeasts and filamentous fungi.
abstract: Sugar cane bagasse hemicellulosic fraction submitted to hydrolytic treatment with 100 mg of sulfuric acid per gram of dry mass, at 140 degrees C for 20 min, was employed as a substrate for microbial protein production. Among the 22 species of microorganisms evaluated, Candida tropicalis IZ 1824 showed TRS consumption rate of 89.8%, net cell mass of 11.8 g L-1 and yield coefficient (Yx/s) of 0.50 g g-1. The hydrolyzate supplemented with rice bran (20.0 g L-1), P2O5 (2.0 g L-1) and urea (2.0 g L-1) provided a TRS consumption rate of 86.3% and a cell mass of 8.4 g L-1. At pH 4.0 cellular metabolism was inhibited, whereas at pH 6.0 the highest yield was obtained. The presence of furfural (2.0 g L-1) hydroxymethylfurfural (0.08 g L-1) and acetic acid (3.7 g L-1) in the hydrolyzate did not interfere with cultivation at pH 6.0.
authors.affiliation: Departamento de Biotecnologia/Faenquil, Lorena/SP, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Pessoa Júnior
authors.forename: I M
authors.initials: IM
authors.lastname: de Mancilha
authors.forename: S
authors.initials: S
authors.lastname: Sato
mesh_terms: D050260:Carbohydrate Metabolism; D003470:Culture Media; D005658:Fungi / Q000254:growth & development* / Q000378:metabolism; D006868:Hydrolysis; D011134:Polysaccharides / Q000378:metabolism*; D015003:Yeasts / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D011134:Polysaccharides; C007916:hemicellulose