Localization of germination-specific spore-lytic enzymes in Clostridium perfringens S40 spores detected by immunoelectron microscopy.
The localization of germination-specific spore-lytic enzymes, an amidase and a muramidase, in Clostridium perfringens S40 spores was examined by immunoelectron microscopy with respective antisera raised against the enzymes and a colloidal gold-immunoglobulin G complex. For both antisera, immunogold particles were visualized on the outside of the cortex of dormant spores, and they were not detected in germinated spores and decoated spores.
Miyata
S
S
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Aichi, Japan.
Kozuka
S
S
Yasuda
Y
Y
Chen
Y
Y
Moriyama
R
R
Tochikubo
K
K
Makino
S
S
D000908:Antibodies, Fungal; D003016:Clostridium perfringens / Q000201:enzymology* / Q000276:immunology; D006026:Glycoside Hydrolases / Q000032:analysis*; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000379:methods; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000032:analysis*; D013172:Spores, Fungal / Q000201:enzymology*
D000908:Antibodies, Fungal; D006026:Glycoside Hydrolases; C026255:endo-N-acetylmuramidase; D009238:N-Acetylmuramoyl-L-alanine Amidase