Axon-regenerating retinal ganglion cells in adult rats synthesize the cell adhesion molecule L1 but not TAG-1 or SC-1.
Retinal ganglion cells (RGCs) in rats regenerate axons in the presence of a PNS nerve graft. To determine if axon-regenerating RGCs synthesize cell adhesion/recognition molecules which they possessed during development, retinae were subjected to in situ hybridization with antisense cRNA probes of L1, TAG-1, and SC-1 (and GAP-43 for comparison). L1 and TAG-1 (and GAP-43) proteins on axons were detected with antibodies. L1, TAG-1, and SC-1 (and Gap-43) mRNAs and L1 and TAG-1 (and Gap-43) proteins were expressed by RGCs in embryonic, postnatal, and adult rats. After optic nerve lesion (ONL), the surviving RGCs between 2 and 28 days after ONL continued to express L1. TAG-1 and SC-1 expression, however is lost. In grafted rats, axon-regenerating RGCs express L1 (together with GAP-43) but neither TAG-1 nor SC-1. Thus, axonal regeneration in grafted rats occurs in the presence of L1 (and GAP-43) but in the absence of TAG-1 and SC-1).
Jung
M
M
Faculty of Biology, University of Konstanz, Germany.
Petrausch
B
B
Stuermer
C A
CA
D020410:Activated-Leukocyte Cell Adhesion Molecule; D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D001369:Axons / Q000502:physiology*; D015816:Cell Adhesion Molecules, Neuronal; D058969:Contactin 2; D004622:Embryo, Mammalian / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005260:Female; D007158:Immunologic Techniques; D017403:In Situ Hybridization; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis*; D009900:Optic Nerve / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012165:Retinal Ganglion Cells / Q000378:metabolism* / Q000502:physiology*; D012584:Sciatic Nerve / Q000637:transplantation
D020410:Activated-Leukocyte Cell Adhesion Molecule; D015816:Cell Adhesion Molecules, Neuronal; D058969:Contactin 2; D016326:Extracellular Matrix Proteins; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules