A chromosome 14q11/TCR alpha/delta specific yeast artificial chromosome improves the detection rate and characterization of chromosome abnormalities in T-lymphoproliferative disorders.
The rate of detection of chromosome abnormalities in T-cell proliferations is lower than that observed in B-cell malignancies. The former frequently involve the TCR alpha/delta locus at chromosome band 14q11. We have identified a YAC encompassing 70% of the TCR alpha/delta locus, which has been used as a fluorescence in situ hybridization probe to detect chromosome rearrangements involving 14q11, both at metaphase and within interphase nuclei, in patients with a variety of T-lymphoproliferative disorders. Its use allowed detection of previously unsuspected TCR alpha/delta rearrangements in 4/13 (30%) immature T-lineage acute leukemias, including two t(10;14) and 2 minor inversion 14s. It also clarified interpretation of complex chromosome 14 abnormalities in mature T-cell proliferations (T-prolymphocytic leukemia and ataxia telangiectasia). Use of this probe will aid the detection and characterization of abnormalities involving the TCR alpha/delta locus, particularly in cases with normal or complex karyotypes and in those proliferations for which mitoses are difficult to obtain.
Rack
K A
KA
Department of Hematology, Hopital Necker Enfants Malades, Paris, France.
Cornélis
F
F
Radford-Weiss
I
I
Bernheim
A
A
Harrison
C J
CJ
Hermine
O
O
Prieur
M
M
Vekemans
M
M
Macintyre
E A
EA
D002869:Chromosome Aberrations; D007446:Chromosome Inversion; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics / Q000648:ultrastructure*; D002999:Clone Cells / Q000737:chemistry / Q000648:ultrastructure; D015342:DNA Probes; D004274:DNA, Recombinant; D005260:Female; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D015458:Leukemia, T-Cell / Q000235:genetics* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008677:Metaphase; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000648:ultrastructure*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000737:chemistry / Q000648:ultrastructure*; D014178:Translocation, Genetic
D015342:DNA Probes; D004274:DNA, Recombinant; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta