Human mini-chromosomes in mouse embryonal stem cells.
We have introduced human mini-chromosomes of 4 Mb and approximately 15 Mb in size into mouse embryonal stem cells. Although these human mini-chromosomes are stable in hamster and chicken cells, they re-arrange or segregate aberrantly in the embryonal stem cells and are rapidly lost in the absence of selection. However, one of the mini-chromosomes re-arranged, acquired mouse centromeric sequences and was then stably maintained for at least 60 population doublings in culture. This mini-chromosome, which is 4 Mb in size, is a candidate for a mouse germ line chromosome vector.
Shen
M H
MH
Cancer Research Campaign Chromosome Molecular Biology Group, Biochemistry Department, Oxford University, UK.
Yang
J
J
Loupart
M L
ML
Smith
A
A
Brown
W
W
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002503:Centromere; D004622:Embryo, Mammalian / Q000166:cytology; D006801:Humans; D051379:Mice; D013234:Stem Cells / Q000378:metabolism; D014998:Y Chromosome