Overproduction of Campylobacter ferritin in Escherichia coli and induction of paracrystalline inclusion by ferrous compound.
The ferritin gene (cft) of Campylobacter jejuni was overexpressed in cells of Escherichia coli using a T7 RNA polymerase expression system. Many round particles which were the same size as the ferritin particles purified from C. jejuni were observed in the lysate of the cft-overexpressed E. coli cells. Since most of them were devoid of a central electron dense core consisting of ferric irons, the Campylobacter ferritins over-produced in E. coli seemed to be apoferritin. When large amounts of ferrous iron (supplied as FeSO4) were added to culture medium, the cft-overexpressed cells formed large inclusion bodies of paracrystalline arrays comprised of ferritin particles with central electron dense cores. The addition of ferric irons did not produce paracrystalline inclusion.
Wai
S N
SN
Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Nakayama
K
K
Takade
A
A
Amako
K
K
D016123:Campylobacter jejuni / Q000235:genetics*; D003460:Crystallization; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000648:ultrastructure; D005293:Ferritins / Q000096:biosynthesis* / Q000235:genetics* / Q000648:ultrastructure; D005798:Genes, Bacterial; D002479:Inclusion Bodies; D016253:Microscopy, Immunoelectron; D011994:Recombinant Proteins / Q000096:biosynthesis; D014764:Viral Proteins
D011994:Recombinant Proteins; D014764:Viral Proteins; D005293:Ferritins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases