[Glycohistochemical analysis of apoptotic bodies in eyelid tumor].
To clarify the relation between the mechanism of apoptosis in tumor tissues and sialic acids on the termini of sugar chains of glycoconjugates, a case of squamous cell carcinoma was examined using immunohistochemistry and glycohistochemistry. Immunohistochemistry and in situ hybridization histochemistry suggested that sialylation by the sialyltransferase in dominant in tumor cells, whereas hydrolysis of sialic acids by the sialidase is dominant in apoptotic bodies. Lectin histochemistry revealed that sialic acid alpha 2, 3 galactose beta 1, 3 N-acetylgalactosamine (Gal beta 1, 3 GalNAc) is present on the surfaces of tumor cells, and Gal beta 1, 3 GalNAc is present on those of apoptotic bodies. The exposed Gal beta 1, 3 GalNAc owing to the decrease in sialic acids on the surfaces of apoptotic bodies may be recognized by the C-type lectin on the macrophage for phagocytosis.
Uehara
F
F
Department of Ophthalmology, Kagoshima University Faculty of Medicine, Japan.
Ohba
N
N
Miyagi
T
T
D000368:Aged; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000648:ultrastructure*; D005142:Eyelid Neoplasms / Q000378:metabolism* / Q000648:ultrastructure*; D005260:Female; D006001:Glycoconjugates / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism
D006001:Glycoconjugates; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid