title: NADPH-diaphorase reactivity is reduced in the molecular layer but increased in the granular layer of primate cerebellum after prolonged anaesthesia.
abstract: To test if nitric oxide (NO) is involved in modulation of neuronal activity after global changes of brain function, nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) reactivity in the cerebellar cortex was compared in monkeys maintained under anaesthesia for 24-48 h with others only anaesthetised for perfusion. After prolonged anaesthesia, NADPH-d activity was reduced in the molecular layer, but increased in the granular layer, with the maintenance of a parasagittal patchy organisation of the highly reactive granule cells. Selective labelling of NADPH-d in the infraganglionic plexuses deep to a subset of Purkinje cell somata was lost in the anaesthetised animals. This differential alteration of NADPH-d reactivity suggests that NO may play a role in regulation of neuronal and synaptic activity during anaesthesia.
authors.affiliation: Department of Anatomy and Neurobiology, University of California, Irvine 92717, USA.
authors.forename: X X
authors.initials: XX
authors.lastname: Yan
authors.forename: L J
authors.initials: LJ
authors.lastname: Garey
mesh_terms: D000758:Anesthesia; D000818:Animals; D002525:Cerebellar Cortex / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D008252:Macaca fascicularis; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D010424:Pentobarbital / Q000008:administration & dosage / Q000494:pharmacology*; D011689:Purkinje Cells / Q000187:drug effects / Q000201:enzymology; D013997:Time Factors
chemical_list: D009252:NADPH Dehydrogenase; D010424:Pentobarbital