title: Experimental otitis media induced by nonviable Moraxella catarrhalis in the guinea pig model.
abstract: Moraxella catarrhalis is a normal resident of the human nasopharyngeal flora, but it is also isolated from middle ear fluid of acute otitis media and otitis media with effusion patients. To determine whether M. catarrhalis has direct pathogenicity in the middle ear, heat-killed M. catarrhalis was inoculated into the middle ear bullae of guinea pigs, and the inflammatory response was investigated. Middle ear mucosal histopathology observed in M. catarrhalis-inoculated ears included subepithelial edema, capillary dilatation, thickening of lamina propria mucosa, inflammatory cell and erythrocyte infiltration into the lamina propria mucosa. Inflammatory cell numbers, lysozyme and myeloperoxidase concentrations in the middle ear washing suspensions of M. catarrhalis-inoculated ears were significantly higher than control ears throughout the experiment. Therefore, nonviable M. catarrhalis induced middle ear inflammation and mucoperiosteal histopathology, which might be caused by direct injury of the nonviable bacteria (e.g. lipooligosaccharide or outer membrane proteins) and metabolic products of inflammatory cells.
authors.affiliation: Department of Otolaryngology, Niigata University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sato
mesh_terms: D000818:Animals; D004432:Ear, Middle / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D004487:Edema / Q000473:pathology; D004848:Epithelium / Q000648:ultrastructure; D006168:Guinea Pigs; D001936:Moraxella catarrhalis / Q000472:pathogenicity*; D009113:Muramidase / Q000378:metabolism; D010033:Otitis Media / Q000201:enzymology / Q000209:etiology*; D009195:Peroxidase / Q000378:metabolism
chemical_list: D009195:Peroxidase; D009113:Muramidase