title: Neomycin- and spectinomycin-resistance replacement vectors for Bacillus subtilis.
abstract: A plasmid is described for Bacillus subtilis that facilitates replacement of the widely used neomycin resistance gene (neo) with a spectinomycin resistance (spcE) gene. A second plasmid is described that facilitates replacement of spcS, associated with mini-Tn10 mutagenesis in B. subtilis, with neo. These plasmids can also function as integrative vectors for B. subtilis. They expand the scope of strain construction and gene analysis in B. subtilis.
authors.affiliation: Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
authors.forename: V K
authors.initials: VK
authors.lastname: Chary
authors.forename: E I
authors.initials: EI
authors.lastname: Amaya
authors.forename: P J
authors.initials: PJ
authors.lastname: Piggot
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D005798:Genes, Bacterial / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D019868:Kanamycin Kinase; D009355:Neomycin / Q000494:pharmacology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011815:R Factors / Q000235:genetics*; D000198:Spectinomycin / Q000494:pharmacology; D014169:Transformation, Bacterial
chemical_list: D000900:Anti-Bacterial Agents; D000198:Spectinomycin; D017853:Phosphotransferases (Alcohol Group Acceptor); D019868:Kanamycin Kinase; D009355:Neomycin