title: Demonstration of Borna disease virus (BDV) in specific regions of the brain from horses positive for serum antibodies to BDV but negative for BDV RNA in the blood and internal organs.
abstract: Sero- and molecular-epidemiological studies on Borna disease virus (BDV) infection show that BDV RNA is not always detected in the peripheral blood mononuclear cells (PBMCs) from serum anti-BDV antibody-positive individuals such as horses, sheep, cattle, cats, and humans. In this study we demonstrated BDV RNA signals by polymerase chain reaction only in restricted regions of the brain from horses with locomotor disease. Four of six horses examined showed apparently positive reactions for anti-BDV antibodies. Specific regions of the brain of these four horses were positive for BDV RNA but the internal organs, lymph nodes, and PBMCs were negative. Histological studies of their brains revealed no apparent histological abnormalities such as inflammatory reactions. These results suggest that BDV chronically infects certain restricted regions of brain in seropositive horses.
authors.affiliation: Section of Serology, Hokkaido University, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hagiwara
authors.forename: N
authors.initials: N
authors.lastname: Momiyama
authors.forename: H
authors.initials: H
authors.lastname: Taniyama
authors.forename: T
authors.initials: T
authors.lastname: Nakaya
authors.forename: N
authors.initials: N
authors.lastname: Tsunoda
authors.forename: C
authors.initials: C
authors.lastname: Ishihara
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D001890:Borna Disease / Q000473:pathology / Q000821:virology*; D001891:Borna disease virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001921:Brain / Q000473:pathology / Q000821:virology*; D006734:Horse Diseases / Q000473:pathology / Q000821:virology*; D006736:Horses; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral