Chaperone SecB from Escherichia coli mediates kinetic partitioning via a dynamic equilibrium with its ligands.
We have shown that the complexes between SecB, a chaperone from Escherichia coli, and two physiological ligands, galactose-binding protein and maltose-binding protein, are in rapid, dynamic equilibrium between the bound and free states. Binding to SecB is readily reversible, and each time the ligand is released it undergoes a kinetic partitioning between folding to its native state and re-binding to SecB. Binding requires that the polypeptide be devoid of tertiary structure; once the protein has folded, it is no longer a ligand. Conditions were established in which folding of the polypeptides was sufficiently slow so that at each cycle of dissociation rebinding was favored over folding and a kinetically stable complex between SecB and each polypeptide ligand was observed. Evidence that the ligand is continually released to the bulk solution and rebound was obtained by altering the conditions to increase the rate of folding of each ligand so that folding of the ligand was faster than reassociation with SecB thereby allowing the system to partition to free SecB and folded polypeptide ligand. We conclude that complexes between the chaperone SecB and ligands are in dynamic, rapid equilibrium with the free states. This mode of binding is simpler than that documented for chaperones that function to facilitate folding such as the Hsp70s and Hsp60s, where hydrolysis of ATP is coupled to the binding and release of ligands. This difference may reflect the fact that SecB does not mediate folding but is specialized to facilitate protein export. Without a requirement for exogenous energy it efficiently performs its sole duty: to keep proteins in a nonnative conformation and thus competent for export.
Topping
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Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington 99164-4660, USA.
Randall
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D018528:ATP-Binding Cassette Transporters; D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000502:physiology*; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000737:chemistry*; D029968:Escherichia coli Proteins; D008024:Ligands; D058619:Maltose-Binding Proteins; D018832:Molecular Chaperones / Q000502:physiology*; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D017510:Protein Folding
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D008024:Ligands; D058619:Maltose-Binding Proteins; D018832:Molecular Chaperones; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; C057842:SecB protein, Bacteria; C023550:galactose-binding protein; C461599:maltose transport system, E coli; D000255:Adenosine Triphosphate