title: Localization of germination-specific spore-lytic enzymes in Clostridium perfringens S40 spores detected by immunoelectron microscopy.
abstract: The localization of germination-specific spore-lytic enzymes, an amidase and a muramidase, in Clostridium perfringens S40 spores was examined by immunoelectron microscopy with respective antisera raised against the enzymes and a colloidal gold-immunoglobulin G complex. For both antisera, immunogold particles were visualized on the outside of the cortex of dormant spores, and they were not detected in germinated spores and decoated spores.
authors.affiliation: Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Aichi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Miyata
authors.forename: S
authors.initials: S
authors.lastname: Kozuka
authors.forename: Y
authors.initials: Y
authors.lastname: Yasuda
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Moriyama
authors.forename: K
authors.initials: K
authors.lastname: Tochikubo
authors.forename: S
authors.initials: S
authors.lastname: Makino
mesh_terms: D000908:Antibodies, Fungal; D003016:Clostridium perfringens / Q000201:enzymology* / Q000276:immunology; D006026:Glycoside Hydrolases / Q000032:analysis*; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000379:methods; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000032:analysis*; D013172:Spores, Fungal / Q000201:enzymology*
chemical_list: D000908:Antibodies, Fungal; D006026:Glycoside Hydrolases; C026255:endo-N-acetylmuramidase; D009238:N-Acetylmuramoyl-L-alanine Amidase