Isolation of mRNAs induced by a hazardous chemical in white-rot fungus, Coriolus versicolor, by differential display.
White-rot fungus Coriolus versicolor, a ligninolytic basidiomycete, has been studied because of its ability to degrade hazardous chemicals. In this study, we searched for genes that are induced by a hazardous chemical using the mRNA differential-display technique and C. versicolor IFO30340 that has been exposed to pentachlorophenol (PCP). Five cDNA fragments were cloned and the DNA sequences of two fragments were analyzed in further detail. The clones corresponded to novel genes that have not previously been identified in C. versicolor. One of the cDNAs exhibited strong sequence homology to the gene for an enolase and the other exhibited homology to a heat shock protein. The expression of the two genes was up-regulated in PCP-treated C. versicolor.
Iimura
Y
Y
Department of Hydrospheric Environmental Protection, National Institute for Resources and Environment, Tsukuba, Ibaraki, Japan. iimura@nire.go.jp
Tatsumi
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000187:drug effects* / Q000235:genetics; D018076:DNA, Complementary; D005656:Fungal Proteins; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal; D015386:Hazardous Substances / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000235:genetics*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010416:Pentachlorophenol / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C107656:FDD123 protein, Coriolus versicolor; D005656:Fungal Proteins; D015386:Hazardous Substances; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D010416:Pentachlorophenol