Histopathologic changes in human small intestine during storage in Viaspan organ preservation solution.
OBJECTIVE
To evaluate the histopathologic changes that occur in human small intestine or time when preserved in Viaspan organ preservation solution.


METHODS
Short segments of human small intestine were placed in standard organ preservation solution (Viaspan) and stored in conditions that mimic the clinical situation associated with clinical organ procurement, preservation, and transplantation. The intestinal segments were removed at sequential time points and placed in 10% formalin. Specimens underwent histopathologic examination to determine time-related changes.


METHODS
Short intestinal segments were obtained from seven multiorgan cadaver donors. Specimens were obtained in a way that exactly mimicked small intestinal organ retrieval.


RESULTS
Small intestinal histology remained normal for the first 6 hours. After 6 hours, vacuolar separation began to occur between the epithelium and the basement membrane in the upper half of the villi. After 9 hours of cold preservation, epithelial detachment extended deep into the crypts with occasional shedding of cells and villi.


CONCLUSIONS
Currently used small intestinal preservation using Viaspan results in considerable histopathologic changes in human jejunum after 9 hours of cold storage. The histopathologic pattern appears normal for the first 6 hours and suggests that preservation times should be limited to this time period when possible.
Tesi
R J
RJ
Department of Surgery, Tulane University Medical Center, New Orleans, La 70112, USA.
Jaffe
B M
BM
McBride
V
V
Haque
S
S
D000241:Adenosine; D000293:Adolescent; D000328:Adult; D000493:Allopurinol; D001485:Basement Membrane / Q000166:cytology; D004847:Epithelial Cells; D005260:Female; D005978:Glutathione; D006801:Humans; D007328:Insulin; D007413:Intestinal Mucosa / Q000166:cytology; D007421:Intestine, Small / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D013997:Time Factors
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose