The use of radionuclide angiography to study blood flow through endothelial cell seeded extrathoracic bypass grafts in the dog.
Endothelial seeding of vascular grafts has been shown to decrease graft thrombogenicity and prolong longevity when implanted in vivo. Previous studies have utilized anatomic grafts to study endothelialization and healing. Anatomic thoracoabdominal grafts do not allow for sequential biopsy for evaluation of individual grafts nor do they approximate the environment for long bypass grafts used in limb salvage. This study evaluated the use of an extra-anatomic aortic bypass graft to assess the healing of endothelial cell seeded expanded polytetrafluoroethylene (ePTFE). Radionuclide angiography was used to evaluate graft patency and quantify blood flow through the graft. Dogs underwent placement of an extra-anatomic 60 cm long, 8 mm internal diameter, graft seeded with autologous endothelium. Grafts were biopsied from 2 weeks up to 1 year. Radionuclide studies were performed postimplantation and following each graft biopsy. Graft placement and biopsies were well tolerated in all dogs. Biopsied segments of graft allowed for sequential studies of the healing of implanted grafts by scanning electron and light microscopy. Flow through the implanted graft was close to 50% of the total caudal abdominal aortic flow. No significant difference in graft flow was noted either between animals or over time.
Sackman
J E
JE
College of Veterinary Medicine, Department of Small Animal Clinical Sciences, University Medical Center, University of Tennessee; Knoxville 37920, USA.
Daniel
G B
GB
Freeman
M B
MB
Lothrop
C D
CD
D000714:Anastomosis, Surgical; D000818:Animals; D001012:Aorta, Abdominal / Q000000981:diagnostic imaging / Q000601:surgery; D001013:Aorta, Thoracic / Q000000981:diagnostic imaging / Q000601:surgery; D001706:Biopsy; D001807:Blood Vessel Prosthesis; D017690:Cell Transplantation; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology*; D005500:Follow-Up Studies; D006083:Graft Occlusion, Vascular / Q000517:prevention & control; D008855:Microscopy, Electron, Scanning; D011138:Polytetrafluoroethylene; D011474:Prosthesis Design; D011875:Radionuclide Angiography; D019275:Radiopharmaceuticals; D012039:Regional Blood Flow; D013670:Sodium Pertechnetate Tc 99m; D013499:Surface Properties; D013927:Thrombosis / Q000517:prevention & control; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014654:Vascular Patency; D014945:Wound Healing
D019275:Radiopharmaceuticals; D011138:Polytetrafluoroethylene; D013670:Sodium Pertechnetate Tc 99m