Menadione-dependent reduction of tertiary amine N-oxide by rat liver cytosol.
This study demonstrates the menadione-dependent reduction of imipramine N-oxide, a tertiary amine N-oxide, to imipramine by rat liver cytosol in the presence of NADH or NADPH. A mechanism for the cytosolic reduction of the tertiary amine N-oxide is proposed. Menadione is converted to its reduced form by a menadione-reducing enzyme such as DT-diaphorase and the reduced pyridine nucleotide, followed by reduction of the tertiary amine N-oxide to the amine by the heme group of catalytic hemoproteins in the presence of reduced menadione as an electron donor.
Kitamura
S
S
Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Japan.
Tatsumi
K
K
D000818:Animals; D018053:Arsenites / Q000494:pharmacology; D002248:Carbon Monoxide / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D001728:Dicumarol / Q000494:pharmacology; D006427:Hemin / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D007099:Imipramine / Q000031:analogs & derivatives* / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D009211:Myoglobin / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000494:pharmacology; D014812:Vitamin K / Q000378:metabolism / Q000494:pharmacology*
D018053:Arsenites; D006454:Hemoglobins; D009211:Myoglobin; D017670:Sodium Compounds; D009243:NAD; D014812:Vitamin K; C017947:sodium arsenite; D009249:NADP; C002921:imipramine N-oxide; D006427:Hemin; D001728:Dicumarol; D002248:Carbon Monoxide; D002374:Catalase; D011190:Potassium Cyanide; D007099:Imipramine