Rapid screening of multiple forensic stains by SSCP analysis of HLA-DQ alpha amplification products.
Single strand conformation polymorphism (SSCP) analysis was performed with HLA-DQ alpha amplification products. The various HLA-DQ alpha genotypes reveal different SSCP patterns. These are reproducible in DNA preparations from unrelated persons with identical HLA-DQ alpha genotype. However, in one of the more frequent HLA-DQ alpha genotypes (1.1/4) two variant SSCP patterns were observed. SSCP analysis allows rapid low-cost screening of many specimens and is an alternative discrimination test of stains beside reverse dot blot hybridization.
Kreike
J
J
Institut für Gerichtliche Medizin, Universität Wien, Austria.
Lehner
A
A
D001826:Body Fluids / Q000737:chemistry*; D005554:Forensic Medicine / Q000379:methods*; D005784:Gene Amplification; D006683:HLA-DQ Antigens / Q000032:analysis* / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D006801:Humans; D009693:Nucleic Acid Hybridization; D018807:Polymorphism, Single-Stranded Conformational; D015203:Reproducibility of Results; D013997:Time Factors
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen