Fluorescent measurement of the intracellular pH during sporulation of Saccharomyces cerevisiae.
This work reports the intracellular pH (pHi) dynamics of Saccharomyces cerevisiae cells in sporulation medium. Cells loaded with the pH-sensitive dye carboxy-seminaphthorhodafluor-1 (C.SNARF-1) exhibited an alkalization of the pHi following the extracellular pH during sporulation in the absence of buffer and almost no change in pHi or delta pH when sporulation was carried out in buffered medium. The results indicate that the pH gradient does not appear to be directly involved in the regulation of acetate uptake during sporulation. However, the alkalization of pHi by eliciting a decrease in metabolic fluxes could account for a lower demand for acetate.
Aon
J C
JC
Instituto Superior de Investigaciones Biológicas (INSIBIO, CONICET-UNT), Departamento de Bioquímica de la Nutrición. o de
Cortassa
S
S
D001578:Benzopyrans; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D009284:Naphthols; D012235:Rhodamines; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000502:physiology*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013172:Spores, Fungal
D001578:Benzopyrans; D005456:Fluorescent Dyes; D009284:Naphthols; D012235:Rhodamines; C068301:carboxy-seminaphthorhodaminefluoride