Effect of testosterone on differential muscle growth and on protein and nucleic acid concentrations in muscles of growing lambs.
Growth, nucleic acid, and protein concentrations were measured in three muscles of 20 rams, 20 wethers, and 20 wethers implanted with testosterone. Two lambs from each group were slaughtered at 14-d intervals from 49 to 133 d, and then at 28-d intervals until 217 d, for a total of 10 slaughter ages. Immediately after slaughter, the semitendinosus, splenius, and triceps brachii muscles were removed, trimmed of adhering fat, and weighed. The DNA, RNA, and protein concentrations of these muscles were determined. Testosterone increased combined weight of the three muscles. The splenius muscles of rams and wethers implanted with testosterone were heavier and had a biphasic growth pattern as the combined muscle weight increased, whereas the splenius muscle of wethers had a single growth phase. Rams and implanted wethers had greater splenius muscle DNA and RNA concentrations than wethers as muscle weight increased. This model could be used to study the gene regulation of testosterone-induced muscle growth with the possibility of invoking similar effects in more economically important muscles.
Arnold
A M
AM
Department of Animal Science, Cornell University, Ithaca, NY 14853-4801, USA.
Peralta
J M
JM
Thonney
M L
ML
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000502:physiology; D004247:DNA / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008297:Male; D008954:Models, Biological; D015233:Models, Statistical; D024510:Muscle Development; D009124:Muscle Proteins / Q000032:analysis* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development; D009696:Nucleic Acids / Q000032:analysis* / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000378:metabolism; D011897:Random Allocation; D012756:Sheep / Q000378:metabolism* / Q000502:physiology; D013739:Testosterone / Q000494:pharmacology*; D013997:Time Factors
D009124:Muscle Proteins; D009696:Nucleic Acids; D013739:Testosterone; D012313:RNA; D004247:DNA