title: A cDNA clone highly expressed in ripe banana fruit shows homology to pectate lyases.
abstract: A cDNA clone (Ban17), encoding a protein homologous to pectate lyase, has been isolated from a cDNA library from climacteric banana fruit by means of differential screening. Northern analysis showed that Ban17 mRNA is first detected in early climacteric fruit, reaches a steady-state maximum at the climacteric peak, and declines thereafter in overripe fruit. Accumulation of the Ban17 transcript can be induced in green banana fruit by exogenous application of ethylene. The demonstrates that expression of this gene is under hormonal control, its induction being regulated by the rapid increase in ethylene production at the onset of ripening. The deduced amino acid sequence derived from the Ban17 cDNA shares significant identity with pectate lyases from pollen and plant pathogenic bacteria of the genus Erwinia. Similarity to bacterial pectate lyases that were proven to break down the pectic substances of the plant cell wall suggest that Ban17 might play a role in the loss of mesocarp firmness during fruit ripening.
authors.affiliation: Departamento de Agrobiología, Centro de Investigación y Desarrollo-Consejo Superior de Investigaciones Científicas, Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Dominguez-Puigjaner
authors.forename: I
authors.initials: I
authors.lastname: LLop
authors.forename: M
authors.initials: M
authors.lastname: Vendrell
authors.forename: S
authors.initials: S
authors.lastname: Prat
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant / Q000378:metabolism*; D004885:Erwinia / Q000201:enzymology; D005638:Fruit / Q000201:enzymology* / Q000502:physiology; D015723:Gene Library; D008969:Molecular Sequence Data; D011058:Pollen / Q000201:enzymology; D011133:Polysaccharide-Lyases / Q000096:biosynthesis* / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D011133:Polysaccharide-Lyases; C023911:pectate lyase