Effect of a topical corticosteroid, a retinoid and a vitamin D3 derivative on sodium dodecyl sulphate induced skin irritation.
Exposure of the skin to sodium dodecyl sulfate (SDS) leads to disruption of barrier and skin irritation. We used repetitive short exposure to a low molarity SDS solution as an in vivo model to mimic the development of irritant contact dermatitis. In this model, we studied clinical (erythema), functional (transepidermal water loss(TEWL)) and cell biological changes. 24 healthy volunteers were patch tested with SDS (0.2%) for 4 h a day for 5 consecutive days. After removal of the patches, the exposed sites were treated 1 X daily either with a topical corticosteroid (triamcinolon acetonide cream 0.05%), a retinoid (tretinoin cream 0.025%), or a vitamin D3 derivative (calcipotriol ointment 50 micrograms/g). Irritant reactions were assessed by erythema scoring and measurement of barrier function with TEWL up to 14 days after the first challenge. Skin biopsies were taken for cell biological changes at day 4. Vehicle-treated sites served as controls. Repetitive exposure of human skin to SDS resulted in a gradual increase in erythema scoring and TEWL associated with the upregulation of proliferative cells as measured by the expression of Ki-67-antigen and of differentiation markers, visualized by increased expression of involucrin and epidermal-fatty-acid binding protein (E-FABP). Skin irritation as assessed by erythema scoring and TEWL was not significantly suppressed by triamcinolone cream. However, a significant reduction of the number of cycling keratinocytes and a decrease in involucrin positive cell layers was observed in this group. Neither treatment with calcipotriol ointment nor with tretinoin cream induced improvement of skin irritation as judged by visual scoring and TEWL. In contrast to steroid treatment, no significant effect of calcipotriol ointment or tretinoin cream treatment was observed with regard to the number of cycling cells and differentiation markers. Further studies are needed to assess whether treatment with topical corticosteroids is an effective modality in skin irritation and irritant contact dermatitis.
Le
T K
TK
Department of Dermatology, University Hospital Nijmegen, The Netherlands.
De Mon
P
P
Schalkwijk
J
J
van der Valk
P G
PG
D000287:Administration, Topical; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001707:Biopsy, Needle; D002117:Calcitriol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002455:Cell Division; D017453:Dermatitis, Irritant / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D003879:Dermatologic Agents / Q000008:administration & dosage*; D005260:Female; D005938:Glucocorticoids; D006801:Humans; D007509:Irritants; D007641:Keratolytic Agents / Q000008:administration & dosage*; D008297:Male; D008875:Middle Aged; D009824:Ointments; D012867:Skin / Q000473:pathology; D012967:Sodium Dodecyl Sulfate / Q000009:adverse effects*; D013501:Surface-Active Agents / Q000009:adverse effects*; D014212:Tretinoin / Q000008:administration & dosage*; D014222:Triamcinolone Acetonide / Q000008:administration & dosage*; D014870:Water Loss, Insensible
D000893:Anti-Inflammatory Agents; D003879:Dermatologic Agents; D005938:Glucocorticoids; D007509:Irritants; D007641:Keratolytic Agents; D009824:Ointments; D013501:Surface-Active Agents; C055085:calcipotriene; D012967:Sodium Dodecyl Sulfate; D014212:Tretinoin; D014222:Triamcinolone Acetonide; D002117:Calcitriol