Genetic to genomic vaccination.
Our development of genetic immunization (unfortunately named 'DNA'-immunization at times) seems to have great promise as a vaccine delivery system. However, one is still left with the often formidable problem of discovering what gene of the pathogen to include in the genetic immunization vector. The is particularly a problem with pathogens with large genomes such as bacteria and parasites. We have developed a potential solution to this problem termed 'expression library immunization'. It involves using expression libraries in genetic immunization vectors to basically strip down the genome into protective genes. It may offer a systematic, unbiased approach to discover vaccine candidates.
Johnston
S A
SA
Department of Internal Medicine, University of Texas-Southwestern Medical Center, Dallas 75235-8573, USA.
Barry
M A
MA
D000818:Animals; D015723:Gene Library; D005822:Genetic Vectors; D016678:Genome; D051379:Mice; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
D019444:Vaccines, DNA