title: Analysis of xysA, a gene from Streptomyces halstedii JM8 that encodes a 45-kilodalton modular xylanase, Xys1.
abstract: The gene xysA from Streptomyces halstedii JM8 encodes a protein of 461 amino acids (Xys1) which is secreted into the culture supernatant as a protein of 45 kDa (Xys1L). Later, this form is proteolytically processed after residue D-362 to produce the protein Xys1S, which conserves the same xylanolytic activity. The cleavage removes a domain of 99 amino acids that shows similarity to bacterial cellulose binding domains and that allows the protein Xys1L to bind to crystalline cellulose (Avicel). Expression of this monocistronic gene is affected by the carbon source present in the culture medium, xylan being the best inducer. By using an anti-Xys1L serum, we have been able to detect xylanases similar in size to Xys1L and Xys1S in most of the different Streptomyces species analyzed, suggesting the ubiquity of these types of xylanases and their processing mechanism.
authors.affiliation: Instituto de Microbiología Bioquímica, Consejo Superior de Investigaciones Científicas (CSIC)/Universidad de Salamanca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ruiz-Arribas
authors.forename: P
authors.initials: P
authors.lastname: Sánchez
authors.forename: J J
authors.initials: JJ
authors.lastname: Calvete
authors.forename: M
authors.initials: M
authors.lastname: Raida
authors.forename: J M
authors.initials: JM
authors.lastname: Fernández-Abalos
authors.forename: R I
authors.initials: RI
authors.lastname: Santamaría
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D015153:Blotting, Western; D002482:Cellulose / Q000378:metabolism; D002874:Chromosome Mapping; D003470:Culture Media / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D010957:Plasmids; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D014990:Xylans; D002482:Cellulose; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; C107879:Xys1 protein, Streptomyces halstedii