title: A new method for assessing masticatory performance: a feasibility and reproducibility study.
abstract: The aim of this study was to develop a method for assessing masticatory performance based on incorporation of a colour binder in a test material with the presumption that the absorption of the colour is proportional to the total surface area of the masticated material. This was tested in vitro with erythrosin as the colour substance and Praestol 655BC as the colour binder incorporated into Satou's synthetic test material (equal amount of carnauba wax and bariumsulphate). The authors found that the difference in spectrophotometrical absorbance between a standard erythrosin solution and the supernatant of the same solution incubated half an hour with artificially pulverized test material was almost linearly correlated (r = 0.997) with the particle surface. This test material was then fabricated as small tablets and four adults chewed repeatedly five grams of these tablets to assess selectivity and reproducibility of the method. A good selectivity was found in that within the total range of the difference in absorbance (0.47-1.19) an inter-individual difference of more than 0.17 is significant on a 95% level with double experiments. There were no differences in the intra-individual variation for double determinations between immediate repeat and 1 week interval performances but the reproducibility was disturbed when two subjects used their partial dentures.
authors.affiliation: Royal Dental College, University of Arhus, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Huggare
authors.forename: B
authors.initials: B
authors.lastname: Skindhöj
mesh_terms: D000042:Absorption; D000180:Acrylic Resins / Q000737:chemistry; D000328:Adult; D001466:Barium Sulfate / Q000737:chemistry; D003116:Color; D003832:Denture, Partial, Removable; D004923:Erythrosine / Q000737:chemistry; D005240:Feasibility Studies; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D016014:Linear Models; D008297:Male; D008334:Mandible; D008409:Mastication / Q000502:physiology*; D008875:Middle Aged; D015203:Reproducibility of Results; D013053:Spectrophotometry; D013499:Surface Properties; D013607:Tablets; D014885:Waxes / Q000737:chemistry; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D001466:Barium Sulfate; D004923:Erythrosine; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D013607:Tablets; D014885:Waxes; D000099166:Polyacrylamides; C026344:carnauba wax