title: Purification of the transcription/repair factor TFIIH and evaluation of its associated activities in vitro.
abstract: We describe here the methodology developed in our laboratory to study the role of TFIIH, a multisubunit protein complex, in the various mechanisms of cell life: transcription, DNA repair, and cell cycle regulation. Protocols are given to purify TFIIH and to study its various enzymatic activities as well as its transcription and nucleotide excision repair activities.
authors.affiliation: Institut de Biologie et Génétique Moléculaire et Cellulaire, UPR 6520 (CNRS), Unité 184 (INSERM), Illkirch, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Marinoni
authors.forename: M
authors.initials: M
authors.lastname: Rossignol
authors.forename: J M
authors.initials: JM
authors.lastname: Egly
mesh_terms: D004260:DNA Repair; D006367:HeLa Cells; D006801:Humans; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
chemical_list: D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH