Suppressive effects of tranilast on the expression of inducible cyclooxygenase (COX2) in interleukin-1beta-stimulated fibroblasts.
We investigated the effects of tranilast on inducible cyclooxygenase (COX2)-mediated prostaglandin E2 (PGE2) production and enzyme induction in interleukin-lbeta (IL-1beta)-stimulated cultured dermal fibroblasts. IL-1beta enhanced PGE2 production in cultured fibroblasts. Tranilast did not affect constitutive cyclooxygenase (COX1) or COX2 activity in non-stimulated or IL-lbeta-stimulated fibroblasts. However, the COX2 expression induced by IL-1beta was inhibited by tranilast. This result, that IL-1beta-induced COX2 expression was suppressed by tranilast, was confirmed by immunohistochemical analysis. Thus, it is possible for tranilast to regulate PGE2 production by inhibiting COX2 induction.
Inoue
H
H
Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
Ohshima
H
H
Kono
H
H
Yamanaka
M
M
Kubota
T
T
Aihara
M
M
Hiroi
T
T
Yago
N
N
Ishida
H
H
D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D051546:Cyclooxygenase 2; D015232:Dinoprostone / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D007527:Isoenzymes / Q000096:biosynthesis*; D007627:Keloid / Q000517:prevention & control; D008565:Membrane Proteins; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D018926:Anti-Allergic Agents; D007375:Interleukin-1; D007527:Isoenzymes; D008565:Membrane Proteins; D062367:ortho-Aminobenzoates; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; C012293:tranilast; D015232:Dinoprostone