Inhibition of ethanol production by Saccharomyces cerevisiae in human blood by sodium fluoride.
Production of ethanol in antemortem blood samples inoculated with an efficient ethanol-producing microorganism and incubated at various temperatures is discussed. Whole blood samples inoculated with Saccharomyces cerevisiae were incubated in gray stoppered Venoject tubes (approximate draw volume 7 mL) containing sodium fluoride (17.5 mg) and potassium oxalate (14.0 mg) at 4 degrees C, 25 degrees C, and 37 degrees C for 0, 24, 96, 192, and 408 h. No volatile substances (such as ethanol, methanol, isopropanol, acetone, or acetaldehyde) (< 0.010 g/dL) were produced in any of the samples at 4 or 25 degrees C. At 24 h incubation a trace amount (< 0.018 g/dL) of ethanol was detected at 37 degrees C.
Amick
G D
GD
Toxicology Department, South Carolina Law Enforcement Division, Columbia, USA.
Habben
K H
KH
D000428:Alcohol Drinking / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D000431:Ethanol / Q000037:antagonists & inhibitors* / Q000097:blood*; D005554:Forensic Medicine; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology*; D014835:Volatilization
D001786:Blood Glucose; D000431:Ethanol; D012969:Sodium Fluoride