title: Prolonged survival of mouse skin allografts in recipients treated with donor splenocytes and antibody to CD40 ligand.
abstract: Combined treatment with antibody against CD40 ligand and one transfusion of donor splenocytes prolonged survival of fully mismatched BALB/c skin allografts on C57BL/6 recipients, with approximately 20% of grafts surviving > 100 days. In vitro alloresponsiveness in treated animals was reduced in the immediate post-transplantation period, but by day 100 was increased despite the presence of a successful allograft. The presence of alloreactivity on day 100 was confirmed in vivo by adoptive transfer, which suggests that our protocol had induced either a state of "split tolerance" or "graft accommodation." Mice with skin grafts that had survived for > or = 100 days revealed no evidence of lymphoid chimerism. Treatment with donor splenocytes and antibody against CD40 ligand permits long-term survival of highly antigenic donor skin allografts despite the presence of functionally intact alloreactive lymphocytes.
authors.affiliation: Diabetes Division, University of Massachusetts Medical School, Biotech 2, Worcester 01605, USA.
authors.forename: T G
authors.initials: TG
authors.lastname: Markees
authors.forename: N E
authors.initials: NE
authors.lastname: Phillips
authors.forename: R J
authors.initials: RJ
authors.lastname: Noelle
authors.forename: L D
authors.initials: LD
authors.lastname: Shultz
authors.forename: J P
authors.initials: JP
authors.lastname: Mordes
authors.forename: D L
authors.initials: DL
authors.lastname: Greiner
authors.forename: A A
authors.initials: AA
authors.lastname: Rossini
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D023201:CD40 Ligand; D002678:Chimera; D004247:DNA / Q000032:analysis; D006084:Graft Rejection / Q000517:prevention & control; D006085:Graft Survival / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000502:physiology; D007108:Immune Tolerance / Q000276:immunology; D008198:Lymph Nodes / Q000737:chemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D016133:Polymerase Chain Reaction; D016038:Skin Transplantation / Q000276:immunology*; D013154:Spleen / Q000737:chemistry / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D023201:CD40 Ligand; D004247:DNA