omega-Conotoxin GVIA and desipramine insensitive norepinephrine efflux from cardiac sympathetic nerve terminal.
Using dialysis technique, prominent accumulation of norepinephrine (NE) in the myocardial interstitial space was observed under local administration of a monoamine oxidase inhibitor (pargyline, 10 mM), and a vesicle uptake inhibitor (reserpine, 10 microM). Accumulation of NE in the myocardial interstitial space was associated with a reduction of dihydroxyphenylglycol levels. Pretreatment with omega-conotoxin GVIA (10 microM) or desipramine (10 microM) did not suppress this response. A brisk increase in dialysate NE levels might occur as a consequence of exocytotic or neurotransporter insensitive efflux of NE.
Yamazaki
T
T
Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
Kawada
T
T
Akiyama
T
T
Kitagawa
H
H
Takauchi
Y
Y
Yahagi
N
N
Sunagawa
K
K
D000320:Adrenergic Fibers / Q000187:drug effects / Q000378:metabolism; D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology*; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002415:Cats; D003891:Desipramine / Q000494:pharmacology*; D005260:Female; D006321:Heart / Q000294:innervation*; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism*; D010293:Pargyline / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D017729:Presynaptic Terminals / Q000187:drug effects / Q000378:metabolism; D012110:Reserpine / Q000494:pharmacology; D020866:omega-Conotoxin GVIA
D018759:Adrenergic Uptake Inhibitors; D002121:Calcium Channel Blockers; D008996:Monoamine Oxidase Inhibitors; D010455:Peptides; D012110:Reserpine; D020866:omega-Conotoxin GVIA; D010293:Pargyline; D003891:Desipramine; D009638:Norepinephrine