title: Regulation of the "tetCD" genes of transposon Tn10.
abstract: In addition to the genes involved in tetracycline resistance, the loop region of the composite transposon Tn10 contains two other known genes, tetC and tetD, whose functions are unclear. Using primarily a genetic approach, we examined tetCD gene expression and regulation. The tetC gene product, TetC, is a diffusible repressor of both tetC and tetD transcription. Despite an earlier claim by others, we do not detect induction of either tetC or tetD by tetracycline (Tc) or several of its analogs. Although the 5' ends of the tetC and tetD messages overlap due to transcription from convergent promoters, we find no evidence for anti-sense RNA control. The operator for the TetC repressor has been localized. We also demonstrate that transcription from the tetD promoter probably terminates within IS10-Right and does not apparently interfere with Tn10 or IS10-Right transposition or its regulation.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Los Angeles, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Pepe
authors.forename: C
authors.initials: C
authors.lastname: Suzuki
authors.forename: C
authors.initials: C
authors.lastname: Laurie
authors.forename: R W
authors.initials: RW
authors.lastname: Simons
mesh_terms: D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic; D019895:Transposases; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C107173:TetC protein, Bacteria; C107174:TetD protein, E coli; D014157:Transcription Factors; C095509:IS10 transposase; D009713:Nucleotidyltransferases; D019895:Transposases; D001616:beta-Galactosidase; D013752:Tetracycline