title: Implementation of conditions of the inoculum stage for Streptosporangium cultures.
abstract: Different factors concerning the inoculum stage of cultures classified within the genus Streptosporangium have been studied. These factors include a comparison among several inoculum media and sources, the influence of aeration and volume of medium in the growth of the cultures, and the effect of the inoculum size on the course of the production of two antibacterial substances. The results suggest that a slight variation in the status of the seed culture (age, biomass concentration, etc.) affect the course of the batch culture during its initial exponential phase (3-5 days), whereas, at longer times, the behavior of the culture does not depend greatly on the characteristics of the inoculum. The results obtained have allowed to define a set of conditions producing the maximum increase of biomass in the minimum time.
authors.affiliation: Centro de Investigación Básica, Merck, Sharp and Dohme de España, Madrid, España. gonzalo_platas@merck.com
authors.forename: G
authors.initials: G
authors.lastname: Platas
authors.forename: J
authors.initials: J
authors.lastname: Collado
authors.forename: H
authors.initials: H
authors.lastname: Martínez
authors.forename: M
authors.initials: M
authors.lastname: Arrese
authors.forename: F
authors.initials: F
authors.lastname: Peláez
authors.forename: M T
authors.initials: MT
authors.lastname: Díez
mesh_terms: D000192:Actinomycetales / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001431:Bacteriological Techniques; D003470:Culture Media; D007218:Industrial Microbiology / Q000379:methods*; D010100:Oxygen; D013171:Spores, Bacterial
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D010100:Oxygen