Identification of sn-2 acetyl glycerophosphocholines in human keratinocytes.
Evidence is accumulating suggesting that platelet-activating factor plays a role in inflammatory dermatoses. Mass spectrometric methods were used to examine the molecular species of sn-2 acetyl glycerophosphocholines (GPC) synthesized by primary cultures of human neonatal foreskin-derived keratinocytes. Ionophore-stimulated keratinocytes synthesize both 1-alkyl and 1-acyl sn-2 acetyl-GPC, and the relative amounts were as follows: hexadecyl > palmitoyl > octadecyl > stearoyl at the sn-1 position. PAF synthesis in the keratinocyte-derived cell line HaCaT was inhibited by dexamethasone, suggesting that the anti-inflammatory effects of glucocorticosteroids in inflammatory dermatoses might be in part related to the inhibition of the synthesis of mediators such as PAF.
Travers
J B
JB
Department of Dermatology, Indiana University School of Medicine, Indianapolis, USA.
Johnson
C
C
Clay
K L
KL
Harrison
K
K
Zekman
T
T
Morelli
J G
JG
Murphy
R C
RC
D000305:Adrenal Cortex Hormones / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D016718:Arachidonic Acid / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D007476:Ionophores / Q000494:pharmacology; D015603:Keratinocytes / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000032:analysis / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
C069030:1-acyl-2-acetyl-sn-glycero-3-phosphocholine; D000305:Adrenal Cortex Hormones; D000893:Anti-Inflammatory Agents; D007476:Ionophores; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D000001:Calcimycin; D003907:Dexamethasone