Enumeration of Enterococcus sp. using a modified mE method.
A modified mE medium (mEI) containing the chromogenic substrate indoxyl-beta-D-glucoside to detect beta-D-glucosidase activity was evaluated with respect to specificity and recovery of enterococci from environmental waters. Extending incubation from 24 to 48 h improved enterococci recovery but 77% of the colonies classified as non-target were confirmed as enterococci. Randomly chosen enterococcal isolates from sewage, exposed in microcosms containing 0.22 micron membrane filtered fresh or estuarine water, exhibited differences in persistence as a function of exposure treatment. Decreasing the concentration of or eliminating indoxyl-beta-D-glucoside from mE did not significantly affect recovery of purified isolates.
Rhodes
M W
MW
Virginia Institute of Marine Science, College of William and Mary, Gloucester Point 23061-1346, USA.
Kator
H
H
D001431:Bacteriological Techniques; D003470:Culture Media / Q000737:chemistry*; D016983:Enterococcus / Q000201:enzymology / Q000302:isolation & purification*; D005618:Fresh Water / Q000382:microbiology*; D005959:Glucosidases / Q000378:metabolism; D005960:Glucosides; D012621:Seasons; D012623:Seawater / Q000382:microbiology*; D012680:Sensitivity and Specificity
D003470:Culture Media; D005960:Glucosides; D005959:Glucosidases