Transient decrease of HPC-1/syntaxin-1A mRNA in the rat hippocampus by kainic acid.
HPC-1/syntaxin-1A is a neuronal protein of which the mRNA has an immediate early gene-like structure in its 3'-untranslated region. Whereas HPC-1/syntaxin-1A protein plays a crucial role in neurotransmitter release, little is known about HPC-1 gene expression. We demonstrate here that HPC-1 mRNA expression in rat hippocampal neurons in vivo decreased 8 h after kainic acid (KA) administration, but was restored thereafter. The transient decrease of HPC-1 mRNA upon KA administration suggests that the HPC-1 mRNA expression in neurons could be altered by excitation by trans-synaptic stimulation.
Fujino
I
I
Department of Physiology, Kyorin University School of Medicine, Tokyo, Japan.
Fujiwara
T
T
Akagawa
K
K
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis*; D015139:Blotting, Southern; D003864:Depression, Chemical; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D017403:In Situ Hybridization; D007274:Injections, Intraperitoneal; D007608:Kainic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D050827:Syntaxin 1
D000954:Antigens, Surface; D018690:Excitatory Amino Acid Agonists; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C494213:Stx1a protein, rat; D050827:Syntaxin 1; D007608:Kainic Acid