Quantitative evaluation of fluorescence in situ hybridization (FISH) signals in uncultured coelomic cells.
Uncultured coelomic cells were hybridized with alpha satellite DNA probes representing chromosomes X, Y, 18, and 13/21 in order to evaluate the distribution of hybridization signals obtained by fluorescence in situ-hybridization (FISH) analysis of this cell type. Cells from 26 samples were hybridized with the X probe and the Y probe was hybridized with cells from 25 of the samples. Cells from 16 and 11 samples were hybridized with an 18 alpha satellite DNA probe and 13/21 alpha satellite probe, respectively. The evaluation demonstrated that FISH with X, Y, 18, and 13/21 alpha satellite DNA probes on uncultured coelomic cells is a reliable technique since the distribution of hybridization signals is comparable to that seen in uncultured amniotic fluid cells.
Crüger
D G
DG
Department of Clinical Genetics, County Hospital of Vejle, Denmark.
Bruun-Petersen
G
G
Kølvraa
S
S
D000653:Amniotic Fluid / Q000166:cytology*; D002882:Chromosomes, Human, Pair 13; D002887:Chromosomes, Human, Pair 18; D002891:Chromosomes, Human, Pair 21; D015342:DNA Probes; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D011247:Pregnancy; D012016:Reference Values; D014960:X Chromosome; D014998:Y Chromosome
D015342:DNA Probes