title: A murine monoclonal anti-N, BIRMA-N, suitable for blood grouping in an automated system.
abstract: BIRMA-N, a murine monoclonal anti-N antibody of the IgG1 subclass, was assessed for suitability as a blood grouping reagent on the Olympus PK7100 automated blood grouping machine. At a selected dilution and over the pH range 5.0-8.0, the antibody performed accurately in this system as confirmed by parallel manual testing of donor blood samples with human anti-N and commercial monoclonal anti-N reagents. These findings show BIRMA-N to be extremely suitable for N typing blood samples in an automated system providing a convenient, objective and cost-effective method for large scale typing of the blood donor population.
authors.affiliation: West Midlands Regional Transfusion Centre, Birmingham, U.K.
authors.forename: J M
authors.initials: JM
authors.lastname: Thompson
authors.forename: D F
authors.initials: DF
authors.lastname: McDonald
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001331:Automation; D001788:Blood Grouping and Crossmatching; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008951:MNSs Blood-Group System / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D008951:MNSs Blood-Group System