Effects of a mechanical stimulation of localization of annexin-like proteins in Bryonia dioica internodes.
Mechanical stimulation exerted by rubbing a young internode of Bryonia dioica plants inhibits its growth. Previous cellular and biochemical studies showed that this growth inhibition is associated with Ca(2+) redistribution and profound modifications of plasma membrane characteristics. We extracted and purified Ca(2+)-dependent phospholipid-binding proteins from B. dioica internodes. Two main proteins, p33 and p35, and other minor bands were isolated and identified as annexin-like proteins because of their biochemical properties and their cross-reactions with antibodies against maize (Zea mays L.) annexins. Rabbit antiserum was obtained by injection of B. dioica p35. This antiserum was used for the immunocytolocalization of annexin-like proteins in internode parenchyma cells. It appeared that the distribution of annexin-like proteins was different before and 30 min after the mechanical stimulation. Western analysis of proteins in membrane fractions after separation by free-flow electrophoresis showed that p35 was present in most fractions, whereas p33 appeared mainly in plasmalemma-enriched fractions after the mechanical stimulation. It is hypothesized that a subcellular redistribution of these proteins might be involved in growth inhibition by mechanical stress.
Thonat
C
C
Laboratoire de Physiologie et Génétique Végétales, Institut National de la Recherche Agronomique-Université Blaise Pascal, Aubière France.
Mathieu
C
C
Crevecoeur
M
M
Penel
C
C
Gaspar
T
T
Boyer
N
N
D000818:Animals; D017302:Annexins / Q000096:biosynthesis* / Q000302:isolation & purification; D000906:Antibodies; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D010812:Physical Stimulation; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D011817:Rabbits
D017302:Annexins; D000906:Antibodies; D010940:Plant Proteins; D002118:Calcium