Human mesenchymal stem cells respond to fibroblast growth factors.
Human mesenchymal stem cells can be isolated from bone marrow aspirates, purified and cultured for many passages without losing their unique properties. One of the hallmarks of stem cells is pluripotency, and human mesenchymal stem cells can be induced to assume phenotypes of mesenchymal tissues including, but not limited to, those of osteocytes, chondrocytes and adipocytes. Due to their ability to form cartilage, bone, fat and other connective tissue, human mesenchymal stem cells have great potential in regenerating diseased or injured tissues. Successful growth of human mesenchymal stem cells is essential to this process, and we have examined the response of human mesenchymal stem cells towards FGF1 and FGF2, two potent growth factors for human tissues. We provide evidence that: 1) human mesenchymal stem cells produce mRNA for receptors for FGF1 and FGF2; 2) these receptors can be detected on the surface of human mesenchymal stem cells; 3) FGF1 and FGF2 increase the rate at which human mesenchymal stem cells proliferate.
van den Bos
C
C
Osiris Therapeutics, Inc., Baltimore, MD 21231-2001, USA.
Mosca
J D
JD
Winkles
J
J
Kerrigan
L
L
Burgess
W H
WH
Marshak
D R
DR
D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000502:physiology*; D005346:Fibroblast Growth Factors / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D013234:Stem Cells / Q000166:cytology*
D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors