Expression of the POU transcription factor Brn-5 inhibits proliferation of NG108-15 cells.
The POU domain transcription factors are a subgroup of homeodomain proteins that appears to control cellular phenotypes. The expression of the POU protein Brn-5 occurs selectively in postmitotic CNS neurons. Ectopic expression of Brn-5 in dividing NG108-15 cells reduces the level of RNA encoding the proliferating cell nuclear antigen (PCNA). This ectopic expression also inhibits DNA synthesis as measured by the incorporation of bromodeoxyuridine (BrdU). Thus, Brn-5 may inhibit the continued proliferation of these cells. A potential function of Brn-5 may be to suppress the action of proliferative signals in postmitotic neurons and thus prevents them from reentering the cell cycle.
Cui
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Department of Pharmacology, University of Maryland School of Medicine, Baltimore 21201, USA.
Bulleit
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RF
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000494:pharmacology*; D015870:Gene Expression; D005910:Glioma / Q000473:pathology*; D051863:Host Cell Factor C1; D009447:Neuroblastoma / Q000473:pathology*; D009474:Neurons / Q000378:metabolism; D050811:Octamer Transcription Factor-1; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D012313:RNA / Q000032:analysis / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000494:pharmacology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D018809:Proliferating Cell Nuclear Antigen; D014157:Transcription Factors; D012313:RNA; D004247:DNA