title: Molecular cloning of human RP105.
abstract: RP105 is a 105-kDa type I membrane protein of the leucine-rich repeat (LRR) family. Anti-RP105 sensitizes B cells to antigen-receptor-mediated apoptosis, but protects B cells from radiation-induced apoptosis and stimulates B cell proliferation. The sequence of the mouse RP105 has been reported. Here, we report the characterization of the human RP105. The 2.6-kb cDNA encodes a protein of 661 amino acids which displays 78% homology with mouse RP105. The 22 LRR and the 9 potential N-linked glycosylation sites within the extracellular region are conserved. While previous studies have shown that RP105 is expressed on surface IgM+IgD+2 B cells in mice, human RP105 was shown to be expressed on all subsets of mature B cells and dendritic cells. Human RP105 gene was mapped to the long arm of chromosome 5, where numerous cytokines and receptors have been localized.
authors.affiliation: Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
authors.forename: I
authors.initials: I
authors.lastname: Fugier-Vivier
authors.forename: O
authors.initials: O
authors.lastname: de Bouteiller
authors.forename: C
authors.initials: C
authors.lastname: Guret
authors.forename: F
authors.initials: F
authors.lastname: Fossiez
authors.forename: J
authors.initials: J
authors.lastname: Banchereau
authors.forename: M G
authors.initials: MG
authors.lastname: Mattei
authors.forename: S
authors.initials: S
authors.lastname: Aït-Yahia
authors.forename: E
authors.initials: E
authors.lastname: Garcia
authors.forename: S
authors.initials: S
authors.lastname: Lebecque
authors.forename: Y J
authors.initials: YJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D015703:Antigens, CD; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D014066:Palatine Tonsil; D017386:Sequence Homology, Amino Acid
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; C092722:CD180 protein, human; C490500:Ly78 protein, mouse; D008565:Membrane Proteins