A- and B-type lamins are differentially expressed in normal human tissues.
A selection of normal human tissues was investigated for the presence of lamins B1, B2, and A-type lamins, using a panel of antibodies specific for the individual lamin subtypes. By use of immunoprecipitation and two-dimensional immunoblotting techniques we demonstrated that these antibodies do not cross-react with other lamin subtypes and that a range of different phosphorylation isoforms is recognized by each antibody. The lamin B2 antibodies appeared to decorate the nuclear lamina in all tissues examined, except hepatocytes, in which very little lamin B2 expression was observed. In contrast to previous studies, which suggested the ubiquitous expression of lamin B1 in mammalian tissues, we show that lamin B1 is not as universally distributed throughout normal human tissues as was to be expected from previous studies. Muscle and connective tissues are negative, while in epithelial cells lamin B1 seemed to be preferentially detected in proliferating cells. These results correspond well with those obtained for lamin B1 in chicken tissues. The expression of A-type lamins is most prominent in well-differentiated epithelial cells. Relatively undifferentiated and proliferating cells in epithelia showed a clearly reduced expression of A-type lamins. Furthermore, most cells of neuroendocrine origin as well as most hematopoietic cells were negative for A-type lamin antibodies.
Broers
J L
JL
Department of Molecular Cell Biology and Genetics, Universiteit Maastricht, The Netherlands.
Machiels
B M
BM
Kuijpers
H J
HJ
Smedts
F
F
van den Kieboom
R
R
Raymond
Y
Y
Ramaekers
F C
FC
D000911:Antibodies, Monoclonal; D004064:Digestive System / Q000378:metabolism; D004702:Endocrine Glands / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D034921:Lamin Type B; D034882:Lamins; D008297:Male; D009132:Muscles / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011233:Precipitin Tests; D012137:Respiratory System / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution; D014566:Urogenital System / Q000378:metabolism
D000911:Antibodies, Monoclonal; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins