title: Purification of active Escherichia coli ribosome recycling factor (RRF) from an osmo-regulated expression system.
abstract: Ribosome release factor (RRF) from Escherichia coli was overproduced from an osmo-expression vector. More than 40% of cell protein was RRF after 6 h of induction. A purification scheme is described that produced 50 mg of RRF from an initial culture of 2 L. The recycling time for ribosomes synthesising the tripeptide fMet-Phe-Leu in vitro in the absence of RF3 was reduced from 40 to 15 s by the addition of purified 1.5 microM RRF.
authors.affiliation: CNRS-UPR 9073, Institut de Biologie Physico-chimique, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: MacDougall
authors.forename: P
authors.initials: P
authors.lastname: Holst-Hansen
authors.forename: K K
authors.initials: KK
authors.lastname: Mortensen
authors.forename: D V
authors.initials: DV
authors.lastname: Freistroffer
authors.forename: M Y
authors.initials: MY
authors.lastname: Pavlov
authors.forename: M
authors.initials: M
authors.lastname: Ehrenberg
authors.forename: R H
authors.initials: RH
authors.lastname: Buckingham
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D011506:Proteins; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012269:Ribosomal Proteins; D014882:Water-Electrolyte Balance
chemical_list: D001426:Bacterial Proteins; D011506:Proteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C087267:ribosome releasing factor