IGF-I enhances neurite regeneration but is not required for its survival in adult DRG explant.
We clarified the roles of insulin-like growth factor (IGF) I and II in peripheral neural regeneration after axotomy using cultured adult DRG explants either with or without associated nerve bundles. When IGF-I was applied at concentrations of 1-10 nM to DRG explants without associated nerve bundles in a serum-free medium, clear enhancement of neural regeneration from both central and peripheral transected nerve terminals was seen. When the same concentrations of IGF-I were applied to DRG explants with associated nerve bundles this enhancement was reduced at central sites and increased at peripheral sites. Neural survival was not affected by IGF-I in any of these culture systems. In comparison with IGF-I, there was no specific effect of IGF-II on neurite regeneration.
Akahori
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Y
Department of Physiology, School of Medicine, Yokohama City University, Japan.
Horie
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H
D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D005727:Ganglia, Spinal / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D007334:Insulin-Like Growth Factor I