Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals.
A set of five monoclonal antibodies (mAb) against porcine major histocompatibility complex (MHC), or swine leukocyte antigens (SLA), class II molecules has been characterized. These mAbs appear to recognize monomorphic determinants on SLA-DR (2F4, 1F12 and 2E9/13) and SLA-DQ (BL2H5 and BL4H2) molecules, as assessed by flow cytometry and immunoprecipitation. By Western blot, the 2F4, 1F12, BL2H5 and BL4H2 epitopes were located on the beta-chains of these molecules. mAbs 2F4 and 1F12 crossreact with leucocytes of dog, cattle, horse and human; mAbs 2E9/13, BL2H5 and BL4H2 bind leucocytes of cattle but not those of human, dog and horse. These mAbs effectively blocked the mixed lymphocyte reaction and the proliferative response to viral antigens (African swine fever virus) and to staphylococcal enterotoxin B. Therefore, these mAbs can be useful reagents for studying MHC class II molecules of pig and crossreactive species, and the immunological processes where they are involved.
Bullido
R
R
Centro de Investigación en Sanidad Animal, INIA, Madrid, Spain.
Doménech
N
N
Alvarez
B
B
Alonso
F
F
Babín
M
M
Ezquerra
A
A
Ortuño
E
E
Domínguez
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D000918:Antibody Specificity; D002417:Cattle; D003429:Cross Reactions; D004285:Dogs; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006736:Horses; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity / Q000276:immunology; D013045:Species Specificity; D013552:Swine / Q000276:immunology*; D014162:Transfection
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II