Evaluation of possible interference of anti-inflammatory drugs upon scintigraphic imaging with macrophage targeting 99mTc-J001X: effects of methylprednisolone, dexamethasone, indomethacin and methotrexate.
J001X, an acylated poly-(1,3)-galactoside isolated from Klebsiella pneumoniae proteoglycan, has been developed to target cells from the monocyte-macrophage lineage. Recent experimental work and initial clinical trials have proved the potential of this molecule labeled with 99mTc for the scintigraphy of inflammatory foci. In a model of radiation-induced inflammation in pigs, the scintigraphic contrast was observed to be very sensitive to a single injection of methylprednisolone given 12 h before scintigraphy. The present study was undertaken to confirm this effect and to estimate the possible interference of various anti-inflammatory agents on the in vivo targeting of macrophages by J001X. Methylprednisolone, dexamethasone, indomethacin and methotrexate used at an immunosuppressive dose were tested to assess the possible risk of false-negative examinations in patients thus treated. Analysis of the results indicated that among the four drugs tested, only methylprednisolone at 0.5-1 mg/kg could interfere with J001X scintigraphy.
Perin
F
F
Laboratoire de Biophysique Cellulaire et RMN, INSERM U316, Faculté de Médecine, Tours, France.
Hoffschir
D
D
Pittet
J C
JC
Daburon
F
F
Normier
G
G
Binz
H
H
Le Pape
A
A
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D004347:Drug Interactions; D005260:Female; D006017:Glycolipids / Q000493:pharmacokinetics*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D008775:Methylprednisolone / Q000494:pharmacology*; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D013552:Swine
D000894:Anti-Inflammatory Agents, Non-Steroidal; D006017:Glycolipids; D007166:Immunosuppressive Agents; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C098281:technetium Tc 99m J001X; D003907:Dexamethasone; D008775:Methylprednisolone; D007213:Indomethacin; D008727:Methotrexate