Molecular cloning and sequence analysis of the dnaA gene of Staphylococcus aureus.
The dnaA gene of Staphylococcus aureus has now been cloned and sequenced. The deduced amino acid sequence of Staphylococcus aureus DnaA protein is 62% and 39% identical to those of the DnaA protein from Bacillus subtilis and Escherichia coli, respectively. Expression of the dnaA gene of Staphylococcus aureus in Escherichia coli caused lethality in an oriC-dependent manner.
Katayama
H
H
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Mizushima
T
T
Miki
T
T
Sekimizu
K
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005804:Genes, Lethal; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria