title: STP2201, a chromosomal promoter sequence of Streptococcus thermophilus.
abstract: Analysis of the structural and functional properties of chromosomal DNA fragments of Streptococcus thermophilus ST128 delineated the promoter sequence STP2201 and identified its -35, -10 and Shine-Dalgarno regions. STP2201 was used in cloning vectors derived from small resident plasmids pER8 (2094 bp) and pER371 (2672 bp) of S. thermophilus strains to facilitate expression of a Streptomyces sp. marker gene (cholesterol oxidase) in lactic acid bacteria. Cell extracts of ST128 transformants converted up to 75% of cholesterol into 4-cholesten-3-one during 8 h of incubation.
authors.affiliation: U.S. Department of Agriculture, ARS, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.
authors.forename: G A
authors.initials: GA
authors.lastname: Somkuti
authors.forename: D K
authors.initials: DK
authors.lastname: Solaiman
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013291:Streptococcus / Q000235:genetics*