title: Disaccharide analysis of skin glycosaminoglycan in localized scleroderma.
abstract: BACKGROUND
  Altered metabolism of skin glycosaminoglycan has rarely been investigated in localized scleroderma in contrast to systemic sclerosis.
  
  
  OBJECTIVE
  The aim of this investigation is to elucidate the change in skin glycosaminoglycan of localized scleroderma.
  
  
  METHODS
  We analyzed 5 skin samples of localized scleroderma and 10 site-matched control skin samples using high performance liquid chromatography after 1-phenyl-3-methyl-5-pyrasolone labeling.
  
  
  RESULTS
  The involved skin constantly showed an increased amount of delta Di-4S(DS), the main disaccharide unit of dermatan sulfate, a decreased amount of delta Di-HA, the main disaccharide unit of hyaluronic acid, and an elevated ratio of delta Di-4S(DS)/delta Di-HA as compared with the uninvolved skin or the site matched control skin.
  
  
  CONCLUSIONS
  These results correlate well with those findings in systemic sclerosis, indicating that the alteration in skin GAG may be closely related to the fibrotic process.
authors.affiliation: Department of Dermatology, Gunma University School of Medicine, Maebashi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yokoyama
authors.forename: O
authors.initials: O
authors.lastname: Ishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Miyachi
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001707:Biopsy, Needle; D046508:Culture Techniques; D004187:Disaccharides / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012594:Scleroderma, Localized / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000378:metabolism / Q000473:pathology
chemical_list: D004187:Disaccharides; D006025:Glycosaminoglycans