Expression of mRNAs of pancreatic and L type RNase inhibitors as a function of age in different tissues of SAMP8 and BDF1 mice.
Turnover of mRNAs could be influenced not only by the synthesis of different mRNA species but also by the altered levels of mRNA-degrading enzymes such as RNases and their endogenous inhibitors. In the present work we evaluated possible age-related changes in the mRNA levels of pancreatic as well as L type RNase inhibitors in five different tissues of the BDF1, SAMR1 and SAMP8 using Northern blots. The mRNA levels varied depending on the tissues and mouse strains studied. In certain instances such as the RNase L inhibitor mRNA levels in the lung of SAMP8, there was a statistically significant (P < 0.05) increase of 40% if we compared the young (3 months old) and old (18 months old) animals. These changes could possibly contribute to a certain extent to the already lower levels of mRNAs due to decreased transcriptional activities in aged animals.
Semsei
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Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Goto
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S
D000375:Aging / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism*; D015870:Gene Expression / Q000235:genetics; D008168:Lung / Q000378:metabolism; D051379:Mice; D010179:Pancreas / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D014018:Tissue Distribution
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D012260:Ribonucleases