title: Constitutive expression of calmodulin-binding phosphoprotein GAP-43 in rat serotonergic and noradrenergic cell groups which project to the spinal cord.
abstract: In situ hybridization was combined with serotonin (5-hydroxytryptamine, 5-HT) or tyrosine hydroxylase immunocytochemistry and with Fluoro-Gold retrograde labeling of bulbo-spinal pathways in order to investigate the expression of GAP-43 mRNA in monoamine cell groups of the adult rat brain stem. Consistent with previous reports, GAP-43 mRNA was observed in serotonin and dopamine cell groups in the pons. In addition, GAP-43 expressing cells were observed in all the major monoamine cell groups in the medulla. Thus the B1, B2 and B3 serotonin cell groups all showed high GAP-43 expression in all contained many GAP-43 expressing serotonin cells with spinal cord projections. The A1, A2, A5 and A6 noradrenaline cell groups also showed high GAP-43 expression, although cells with spinal cord projections were largely restricted to the A5 group and A6 subcoeruleus region. In all areas, GAP-43 expressing cells with spinal cord projections were also observed which were not serotonergic or noradrenergic.
authors.affiliation: Department of Anatomy, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, London.
authors.forename: G
authors.initials: G
authors.lastname: Wotherspoon
authors.forename: J J
authors.initials: JJ
authors.lastname: López-Costa
authors.forename: G J
authors.initials: GJ
authors.lastname: Michael
authors.forename: J V
authors.initials: JV
authors.lastname: Priestley
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000096:biosynthesis* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D019922:GAP-43 Protein; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000378:metabolism; D009638:Norepinephrine / Q000502:physiology*; D015345:Oligonucleotide Probes; D011149:Pons / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism / Q000502:physiology*; D013116:Spinal Cord / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D002148:Calmodulin-Binding Proteins; D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012701:Serotonin; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine; D009638:Norepinephrine