A cell cycle-regulated histone H3 gene of alfalfa with an atypical promoter structure.
The control of cell cycle expression of histone genes in plants is incompletely understood. A new histone H3 gene was cloned from alfalfa (Medicago sativa) that codes for the replication-dependent histone H3.1 variant protein. Despite lacking all promoter sequence motifs that have been associated with cell cycle-dependent histone gene expression in plants, northern analysis of synchronized cells clearly linked gene expression to DNA replication. TTAATNA was recognized as a new sequence element in the 3' untranslated regions of this and all other cell cycle-dependent histone H3 genes of dicotyledonous plants. It is not found in the replication-independent histone H3 genes.
Robertson
A J
AJ
Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill 27599-7100, USA.
Kapros
T
T
Waterborg
J H
JH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics*; D003001:Cloning, Molecular; D006224:Cricetinae; D005260:Female; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000235:genetics*; D000455:Medicago sativa / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D006657:Histones