Influence of Photofrin on the hematopoietic accessory function of murine peritoneal cells.
The porphyrin photochemotherapeutic agent Photofrin stimulates hematopoietic activity within the bone marrow (BM) and spleens of normal mice. We found that the intraperitoneal (i.p.) administration of Photofrin also caused a 3-4 fold increase in peritoneal cell (PC) numbers, particularly cells bearing granulocyte surface antigens. Little granulocyte-macrophage progenitor activity was detectable within the PC population of control and Photofrin-injected mice suggesting that Photofrin had elicited an influx of inflammatory cells into the region. In contrast to cells from control animals, PC obtained 6 h to 168 h after the i.p. injection of Photofrin exhibited a consistently inferior capacity to support the growth of BM colony forming units granulocyte-macrophage (CFU-GM). When PC from control or Photofrin-treated mice were added to the BM culture system in the presence of defined growth factors there was no effect on the number of colonies formed. This finding indicated that negative regulatory elements were not responsible for the reduced hematopoietic accessory activity exhibited by PC from Photofrin-treated mice. Supernatants conditioned by PC from Photofrin-injected mice poorly supported BM CFU-GM growth in vitro and in contrast to PC supernatants prepared from control mice did not contain detectable amounts of granulocyte-macrophage colony stimulating factor (GM-CSF). The cellular changes which occur within the peritoneal cavity represent bystander events not directly related to the hematostimulatory action of Photofrin.
Hunt
D W
DW
QLT Photo Therapeutics, Inc., Vancouver, British Columbia, Canada.
Levy
J G
JG
D000818:Animals; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis / Q000187:drug effects* / Q000276:immunology; D017324:Hematoporphyrin Derivative / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007958:Leukocyte Count / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010529:Peritoneal Cavity / Q000166:cytology*
D017324:Hematoporphyrin Derivative