A classic cAMP responsive element in the promoter region of the alpha 1-GABAA receptor subunit has non-classic properties.
The cAMP responsive element binding protein (CREB) and the glucocorticoid receptor (GR) have been reported to bind to a 60 bp promoter fragment of the alpha 1-GABAA receptor gene containing a classic cAMP-responsive element (CRE). We inserted this fragment into a hormone responsive element-deleted mouse mammary tumor virus promoter controlling the expression of luciferase. Activation of GR showed no significant change in luciferase expression, but hormone induction by forskolin revealed a reduction in neuronal cell lines. Furthermore, we demonstrate that cellular factors from neuronal cells can bind to the CRE-containing promoter fragment, although competition by unlabeled CRE and GRE oligo-nucleotides is not present. Mutation of the CRE site and deletion of neighboring DNA sequences indicate that the promoter is probably associated with a complex of different regulatory factors.
Gerner
F M
FM
Max Planck Institute of Psychiatry, Clinical Institute, Munich, Germany.
Trapp
T
T
Hauser
C A
CA
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry* / Q000502:physiology; D015870:Gene Expression; D008156:Luciferases / Q000096:biosynthesis; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D018079:Receptors, GABA / Q000737:chemistry* / Q000502:physiology; D014162:Transfection; D001616:beta-Galactosidase / Q000737:chemistry
D002373:Cyclic AMP Receptor Protein; D018079:Receptors, GABA; D008156:Luciferases; D001616:beta-Galactosidase