The PRINS technique: potential use for rapid preimplantation embryo chromosome screening.
The primed in-situ labelling (PRINS) method is an alternative to in-situ hybridization for chromosomal detection based on the use of chromosome-specific oligonucleotide primers. Using this process, we have developed a simple and semi-automatic method for rapid in-situ detection of human chromosomes. The reaction was performed on a programmable temperature cycler. Specific labelling was obtained in < 2 h reaction. Double PRINS techniques were performed on six morphologically abnormal preimplantation embryos using primers specific for chromosomes 9, 16, 18, 21, X and Y. The majority of these embryos displayed chromosomal abnormalities. The present results demonstrate that PRINS may be a simple and reliable technique applicable in human preimplantation diagnosis.
Pellestor
F
F
CNRS UPR 9008, Montpellier, France.
Girardet
A
A
Andréo
B
B
Lefort
G
G
Charlieu
J P
JP
D001755:Blastocyst / Q000166:cytology / Q000648:ultrastructure*; D001757:Blastomeres / Q000648:ultrastructure; D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D002877:Chromosomes, Human / Q000648:ultrastructure; D003581:Cytodiagnosis / Q000379:methods*; D017931:DNA Primers; D004276:DNA, Satellite; D005307:Fertilization in Vitro; D005456:Fluorescent Dyes; D005819:Genetic Markers; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004276:DNA, Satellite; D005456:Fluorescent Dyes; D005819:Genetic Markers