Methionine oxidation and apoptosis induction by ascorbate, gallate and hydrogen peroxide.
Addition of either ascorbate, gallate, caffeate or hydrogen peroxide to the culture medium stimulated the oxidation of free methionine to methionine sulfoxide, regardless of the presence or absence of the cells. In methionine-free medium, growth of human myelogenous leukemic cell lines was nearly stopped by G1 arrest, without induction of internucleosomal DNA cleavage. Methionine sulfoxide, a major oxidation product of methionine, was neither growth-promoting nor cytotoxic. On the other hand, methional, a deamination and decarboxylation product of methionine, was highly cytotoxic. The present study suggests that the apoptosis induction by these antioxidants and oxidant cannot simply be explained by methionine oxidation or depletion.
Sakagami
H
H
Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Satoh
K
K
Kadofuku
T
T
Takeda
M
M
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002109:Caffeic Acids / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005707:Gallic Acid / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008715:Methionine / Q000378:metabolism*; D010084:Oxidation-Reduction; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D002109:Caffeic Acids; D005707:Gallic Acid; D008715:Methionine; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; C040048:caffeic acid