Natural occurrence of 2'-phospho-cyclic ADP ribose in mammalian tissues.
2'-Phospho-cyclic ADP-ribose (P-cADPR) is a newly identified Ca2+-mobilizing agent derived from NADP that stimulates intracellular Ca2+ release by a mechanism distinct from inositol 1, 4, 5-trisphosphate. In this report, we show that P-cADPR is an endogenous metabolite in bovine tissues with basal levels ranging from 17.6 to 89.5 fmol/mg protein. The natural occurrence of this Ca2+-mobilizing nucleotide provides a potential link between NADP(H) metabolism and regulation of Ca2+ homeostasis.
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Division of Medicinal Chemistry and Pharmaceutics, College of Pharmacy, University of Kentucky, Lexington 40536, USA.
Coyle
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Jacobson
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D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D036563:Cyclic ADP-Ribose / Q000031:analogs & derivatives*; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D009249:NADP / Q000378:metabolism*; D009928:Organ Specificity; D011863:Radioimmunoassay; D012016:Reference Values; D013154:Spleen / Q000737:chemistry
C098663:cyclic ADP-ribose 2'-phosphate; D036563:Cyclic ADP-Ribose; D000246:Adenosine Diphosphate Ribose; D009249:NADP; D002118:Calcium