Determination of standard zinc values in the intact tissues of mice by ICP spectrometry.
The most commonly encountered difficulties for the quantitative measurement of zinc in biological samples are the limited sample amount, total and effective digestion of connective and fatty residues, and low zinc concentrations. These problems often lead to the determination of lower zinc values than actually present, so that the sample preparation, digestion, and analytical procedure deserve careful attention. In this short communication, a new method for microwave tissue disintegration is described. The authors have obtained consistent and reproducible results with tissue samples of 0.5 g or less.
Verbanac
D
D
Medical Faculty, Department of Chemistry and Biochemistry, University of Rijeka, Croatia.
Milin
C
C
Domitrović
R
R
Giacometti
J
J
Pantović
R
R
Ciganj
Z
Z
D000818:Animals; D001923:Brain Chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008872:Microwaves; D009132:Muscles / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D013052:Spectrometry, X-Ray Emission; D013054:Spectrophotometry, Atomic; D013154:Spleen / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry; D014018:Tissue Distribution; D015032:Zinc / Q000032:analysis* / Q000097:blood
D015032:Zinc