Patterning the expression of a tissue-specific transcription factor in embryogenesis: HNF1 alpha gene activation during Xenopus development.
Tissue-specific transcription factors play an essential role in establishing cell identity during development. We review our knowledge of the molecular events involved in the activation of the gene encoding the tissue-specific transcription factor HNF1 alpha (LFB1). The available data suggest that the maternal factors OZ-1, HNF4 alpha and HNF4 beta act as initial activators of the HNF1 alpha promoter. We present evidence suggesting that the mesoderm-inducing factor activin A plays a critical role by acting through the HNF4 binding site of the HNF1 alpha promoter. The activity of this embryonic morphogen seems to form a gradient opposing the distribution of the maternal HNF4 proteins that are concentrated at the animal pole of the egg. After zygotic gene transcription the HNF1 alpha-related transcription factor HNF1 beta accumulates faster than HNF1 alpha itself and thus is likely to contribute to the activation of the HNF1 alpha transcription via the HNF1 binding site. The cofactor of the HNF1 proteins (DCoH) is present throughout development and thus cannot limit the activation potential of HNF1 alpha in early development. Our results provide a detailed description of setting up the expression pattern of a tissue-specific transcription factor during embryogenesis.
von Strandmann
E P
EP
Universitätskinikum Essen, Institut für Zellbiologie (Tumorforschung), Germany.
Nastos
A
A
Holewa
B
B
Senkel
S
S
Weber
H
H
Ryffel
G U
GU
D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins; D019143:Evolution, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051537:Hepatocyte Nuclear Factor 1; D051538:Hepatocyte Nuclear Factor 1-alpha; D051539:Hepatocyte Nuclear Factor 1-beta; D051557:Hepatocyte Nuclear Factor 4; D007265:Inhibins / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation; D014981:Xenopus / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029867:Xenopus Proteins
D004268:DNA-Binding Proteins; D051538:Hepatocyte Nuclear Factor 1-alpha; D051557:Hepatocyte Nuclear Factor 4; C000627649:Hnf1b protein, Xenopus; D009687:Nuclear Proteins; D010750:Phosphoproteins; D014157:Transcription Factors; D029867:Xenopus Proteins; C496490:hnf1a protein, Xenopus; D028341:Activins; D051537:Hepatocyte Nuclear Factor 1; D051539:Hepatocyte Nuclear Factor 1-beta; D007265:Inhibins