Generation of monoclonal antibodies against a feline CD antigen (CD4) expressed by a recombinant baculovirus.
We attempted to establish a system to generate monoclonal antibodies (mAbs) recognizing CD antigens on lymphocytes of domestic animals using the expressed CD antigens by a baculovirus expression system. For this purpose, we selected feline CD4 (fCD4) antigen and expressed it in an insect cell line (Sf9 cells). To obtain mAbs, BALB/c mice were immunized with feline peripheral blood mononuclear cells (fPBMCs) or Sf9 cells expressing the fCD4 (Sf-fCD4 cells), and then hybridomas secreting antibodies were screened by indirect immunofluorescence assay against the opposite antigens of Sf-fCD4 cells or fPBMCs, respectively. Five mAbs recognizing the fCD4 were obtained in total. The system established here might be useful to obtain mAbs recognizing CD antigens of domestic animals.
Shimojima
M
M
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Morikawa
S
S
Maeda
K
K
Tohya
Y
Y
Miyazawa
T
T
Mikami
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D016367:Baculoviridae / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology*; D002415:Cats; D002460:Cell Line; D007313:Insecta; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D011994:Recombinant Proteins