Glucose transport in a murine mammary epithelial cell line.
The glucose transport systems of the COMMA-D cell line (a murine mammary epithelial cell line) were examined using 2-deoxyglucose as substrate. The kinetics and inhibition studies with other sugars including xylose suggested that the transport system had properties of both GLUT-1 and Glut-3. Subsequent analysis of mRNA transcripts using cDNAs for GLUT-1 to 4 showed that only GLUT-1 was expressed in the COMMA-D cells. The results highlight the fact that kinetic and substrate specificity are not sufficient, by themselves, for the identification and characterisation of GLUT isoforms in cultured cells.
Bennett
B L
BL
Department of Biochemistry, University of Otago, Dunedin, New Zealand. brydon.l.bennett@am.pnu.com
Grigor
M R
MR
Prosser
C G
CG
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D050260:Carbohydrate Metabolism; D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004187:Disaccharides / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D010693:Phloretin / Q000494:pharmacology; D011247:Pregnancy; D013379:Substrate Specificity
D004187:Disaccharides; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; C495678:Slc2a1 protein, mouse; C495689:Slc2a3 protein, mouse; C495693:Slc2a4 protein, mouse; D003571:Cytochalasin B; D003847:Deoxyglucose; D005947:Glucose; D010693:Phloretin