Exercise test: trend of the leukocyte flow properties, polymorphonuclear membrane fluidity and cytosolic Ca2+ content in normals, in subjects with previous acute myocardial infarction and in subjects with aortocoronary by-pass.
We evaluated, during an exercise test, the leukocyte flow properties, the polymorphonuclear leukocyte (PMN) membrane fluidity and PMN cytosolic Ca2+ content in normals, in subjects with previous acute myocardial infarction (AMI) and in subjects previously submitted to a aortocoronary by-pass. Leukocyte flow properties were evaluated using the St. George filtrometer. Examination of the PMN membrane fluidity was effected employing the probe TMA-DPH; while evaluation of the PMN cytosolic Ca2+ content was carried out using the probe Fura 2-AM. At baseline, in both cardiopathic groups a significant difference in PMN filtration parameters and in PMN cytosolic Ca2+ content was evident compared to normals. In normals, at peak of exercise, there was an evident reduction of mononuclear filtration parameters, while during recovery a slight increase of the PMN cytosolic Ca2+ content was observed. In subjects with previous AMI and in subjects with aortocoronary by-pass, however, we observed, at peak of exercise, a decrease of the mononuclear filtration parameters, a reduction of the PMN membrane fluidity and an increase of the PMN cytosolic Ca2+ content. In both groups, the changes in PMN membrane fluidity and cytosolic Ca2+ content remained during recovery. The trend of the PMN membrane fluidity and cytosolic Ca2+ content found in the cardiopathic subjects during the exercise test suggest the PMN activation may be more evident in these subjects.
Caimi
G
G
Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo, Italy.
Assennato
P
P
Canino
B
B
Montana
M
M
Ventimiglia
G
G
Lo Presti
R
R
D000328:Adult; D000368:Aged; D002118:Calcium / Q000097:blood*; D003289:Convalescence; D001026:Coronary Artery Bypass; D003327:Coronary Disease / Q000097:blood* / Q000601:surgery; D003600:Cytosol / Q000737:chemistry; D005080:Exercise Test; D018056:Hemorheology; D006801:Humans; D007962:Leukocytes / Q000145:classification; D008297:Male; D008560:Membrane Fluidity; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood*; D009504:Neutrophils / Q000166:cytology*
D002118:Calcium