Effect of experimental infection with Listeria monocytogenes on the development of pregnancy and on concentrations of progesterone, oestrone sulphate and 15-ketodihydro-PGF2 alpha in the goat.
The effect of Listeria monocytogenes infection on hormone levels in pregnant goats was studied. Four goats (Group I) received an intravenous inoculation of a bacterial culture (Type 1) on Days 69-77 and another four goats (Group II) received a similar inoculation on Days 105-106 of gestation. Five non-inoculated goats were used as controls. Plasma was analysed for progesterone, oestrone sulphate and 15-ketodihydro-PGF2 alpha. The status of the foetus was followed using real-time ultrasonography. Three of the four goats in Group I aborted 8-10 days after inoculation. The fourth goat gave birth to a normal live kid at term. The three goats which aborted showed clinical signs of disease in connection with abortion. In Group II, all goats aborted after 9-11 days. All the goats showed clinical symptoms of disease from a few days after inoculation and the symptoms continued until abortion. The clinical symptoms of disease were more pronounced in Group II than in Group I. L. monocytogenes was isolated from all aborted foetuses. None of the control goats aborted. Ultrasound examination revealed foetal death either immediately before or up to 2 days before abortion. Mummification had begun in the foetus that had been dead for 2 days before expulsion. In comparison with pre-inoculation plasma levels in Group I, a significant decrease (P < 0.05) in progesterone levels and an increase in 15-ketodihydro-PGF2 alpha levels were observed from Days 4 and 6 after inoculation, respectively. In Group II, a significant decrease (P < 0.05) in progesterone levels and an increase in 15-ketodihydro-PGF2 alpha levels in plasma were observed from Days 8 and 6, respectively. The oestrone sulphate levels decreased slightly in the inoculated goats a few days before abortion. The pattern of changes in levels around abortion was similar to the pattern present in the control animals around parturition. However, oestrone sulphate levels did not increase in the inoculated groups before abortion in contrast to goats which delivered healthy kids. The changes in levels of 15-ketodihydro-PGF2 alpha in goats that aborted indicated that the endocrine foetal-placental function was disturbed, which was most likely due to the establishment and development of L. monocytogenes in the placenta and foetus.
Engeland
I V
IV
Department of Reproduction and Forensic Medicine, Norwegian College of Veterinary Medicine, Oslo, Norway.
Waldeland
H
H
Ropstad
E
E
Kindahl
H
H
Andresen
O
O
D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D004970:Estrone / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006041:Goats / Q000382:microbiology* / Q000502:physiology; D008088:Listeriosis / Q000503:physiopathology / Q000662:veterinary*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000503:physiopathology / Q000662:veterinary*; D011374:Progesterone / Q000097:blood*
C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D004970:Estrone; D011374:Progesterone; D015237:Dinoprost; C017296:estrone sulfate