title: Immunohistochemical analysis of 4-aminobiphenyl-DNA adducts in oral mucosal cells of smokers and nonsmokers.
abstract: BACKGROUND
  The "biologically effective dose markers", DNA and protein adducts, are a direct index of carcinogen induced cell damage and an indirect one of genetic susceptibility. This study aimed to examine the dose-response relationship for 4-Aminobiphenyl-DNA adducts in oral cells of smokers and non smokers.
  
  
  METHODS
  An immunoperoxidase method with the monoclonal 3C8 antibody, which recognizes 4-Aminobiphenyl-DNA, has been used for detecting DNA damage in oral cells of 12 smokers and 12 non smokers.
  
  
  RESULTS
  Higher staining for 4-Aminobiphenyl-DNA was detected in the cells of smokers (187 +/- 42) vs. non smokers (135 +/- 35) (p = 0.004), with a twofold range in relative staining for both groups, suggesting individual differences relevance in metabolizing carcinogens and/or repairing DNA damage.
  
  
  CONCLUSIONS
  This non invasive method requiring small cell amounts is a tool for monitoring large groups of subjects at risk in primary prevention programs.
authors.affiliation: Institute of General Pathology, Centro di Ricerche Oncologiche Giovanni XXIII, Catholic University School of Medicine, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Romano
authors.forename: R
authors.initials: R
authors.lastname: Mancini
authors.forename: P
authors.initials: P
authors.lastname: Fedele
authors.forename: G
authors.initials: G
authors.lastname: Curigliano
authors.forename: G
authors.initials: G
authors.lastname: Flamini
authors.forename: M R
authors.initials: MR
authors.lastname: Giovagnoli
authors.forename: N
authors.initials: N
authors.lastname: Malara
authors.forename: A
authors.initials: A
authors.lastname: Boninsegna
authors.forename: A
authors.initials: A
authors.lastname: Vecchione
authors.forename: R M
authors.initials: RM
authors.lastname: Santella
authors.forename: A
authors.initials: A
authors.lastname: Cittadini
mesh_terms: D000328:Adult; D000428:Alcohol Drinking; D000611:Aminobiphenyl Compounds / Q000032:analysis*; D018736:DNA Adducts / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry*; D012907:Smoking
chemical_list: D000611:Aminobiphenyl Compounds; D018736:DNA Adducts