title: Effect of blood storage on erythrocyte/wall interactions: implications for surface charge and rigidity.
abstract: In this report, we study, under flow conditions, the interactions of stored erythrocytes with an artificial surface: a microelectrode whose charge density ranges from -15 to +27 microC/cm2. Interactions consist of red cells slowly circulating on the microelectrode and exerting a real contact with the electrode. Interaction is detected and measured by transient fluctuations of the electrolyte resistance obtained by impedance measurement of the microelectrode. Effects of aging induced by storage of whole blood at 4 degrees C show that the surface charge of erythrocytes rapidly decreases when blood is stored for more than 6 days under our experimental conditions. In comparison with trypsin-treated erythrocytes, an eight day storage induces a 60% decrease in the surface charge of red cells. After two weeks of storage, red cells are no longer negatively charged, presumably because of removal of sialic acid. Cells rigidity is significant after 6 days of storage and influences the electrical contact. Membrane rigidity increase could arise from the surface charge decrease. Finally the surface charge decrease could be importance in the use of stored blood.
authors.affiliation: Laboratoire de Biorhéologie et d'Hydrodynamique Physicochimique, CNRS URA 343, Université Paris 7, France.
authors.forename: C
authors.initials: C
authors.lastname: Godin
authors.forename: A
authors.initials: A
authors.lastname: Caprani
mesh_terms: D001793:Blood Preservation; D017097:Electric Impedance; D004907:Erythrocyte Deformability / Q000502:physiology; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D013499:Surface Properties; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
chemical_list: D014357:Trypsin