Use of microdialysis for in-vivo monitoring of hydroxyl free-radical generation in the rat.
Free-radicals are reported to cause the tissue-damage associated with some toxins and diseases, yet there is no suitable method for routine in-vivo monitoring of these species. This paper introduces an in-vivo microdialysis technique in which the hydroxyl radical reacts with salicylate to generate dihydroxybenzoic acids (DHBA) which are measured by HPLC with electrochemical detection. When pargyline, a monoamine oxidase inhibitor, was infused into rat brain, the levels of DHBA increased markedly. When noradrenaline was administered to animals pre-treated with pargyline, DHBA levels increased markedly compared with the group treated with noradrenaline only. When the heart was subjected to 15-min regional ischaemia by occlusion of the left anterior descending coronary artery, levels of DHBA in heart dialysate were unchanged. Electrical stimulation of the stellate ganglion resulted in marked elevation of levels of DHBA the myocardial dialysate. Infusion of Fe2+ into rat liver resulted in increased formation of DHBA. When the intestine was rendered ischaemic for 10, 20 and 30 min, the highest DHBA level was obtained after 10-min ischaemia and the lowest after 30 min. These results confirm that free-radical production might make a major contribution at certain stages in the progression of the injury.
Obata
T
T
Department of Pharmacology, Oita Medical University, Japan.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004558:Electric Stimulation; D005841:Gentisates; D006861:Hydrogen Peroxide / Q000378:metabolism; D062385:Hydroxybenzoates / Q000032:analysis / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology; D007511:Ischemia / Q000378:metabolism; D007583:Jejunum / Q000098:blood supply / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism; D012459:Salicylates / Q000737:chemistry / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000502:physiology
D005841:Gentisates; D062385:Hydroxybenzoates; D008996:Monoamine Oxidase Inhibitors; D012459:Salicylates; D013481:Superoxides; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid; D010293:Pargyline; D006861:Hydrogen Peroxide; D007501:Iron; D008995:Monoamine Oxidase; C010925:2,5-dihydroxybenzoic acid; D009638:Norepinephrine