Establishment and characterization of a new human glioblastoma cell line (MGM-1) with highly motile phenotype.
A new cell line MGM-1 was established from a primary tumor of the left temporal lobe with histological diagnosis of glioblastoma multiforme, removed from a 64-year-old Japanese male. The patient died of recurrence and unusual extracranial metastases of the tumor 7 months after the surgery. The cultured MGM-1 cells are spindle or polygonal in shape. After serial passages, glial fibrillary acidic protein became negative immunocytochemically in vitro. The modal chromosome number was 61-64. Doubling time and soft agar colony forming efficiency were 42.9h and 0.4%, respectively (at 25th passage). MGM-1 is a highly motile cell line in vitro and its serum-free conditioned medium is chemotactic and chemokinetic for other glioma cells. Secretion of gelatinases (probably MMP-2/72-kDa type i.v. collagenase) and MMP-9/92-kDa type i.v. collagenase) and urokinase-type plasminogen activator were also investigated. MGM-1 would therefore be useful for studying the mechanisms regulating glioma-cell motility and invasion. The MGM-1 cell line has been propagated continuously by serial passages (more than 100 passages) during the past 4 years.
Moriyama
T
T
Department of Neurosurgery, Miyazaki Medical College.
Kataoka
H
H
Seguchi
K
K
Nabeshima
K
K
Kawano
H
H
Goya
T
T
Wakisaka
S
S
Ohgami
A
A
Suzumiya
J
J
Kikuchi
M
M
Koono
M
M
D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology; D002455:Cell Division; D002465:Cell Movement / Q000235:genetics; D002633:Chemotaxis; D018093:Gelatinases / Q000096:biosynthesis; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology; D006801:Humans; D007621:Karyotyping; D008297:Male; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000096:biosynthesis; D008875:Middle Aged; D010641:Phenotype; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis
D014568:Urokinase-Type Plasminogen Activator; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2