title: [Cytokine producing ability of peripheral blood mononuclear cells in the clinical course of pulmonary tuberculosis].
abstract: Interferon-gamma (IFN-gamma) and interleukin-10 (IL-10)-producing ability of peripheral blood plastic-dish adherent cells and non-adherent cells obtained from patients with active pulmonary tuberculosis (N = 17) and healthy controls (N = 14) upon stimulation with purified protein derivatives (PPD) were assessed. Adherent cells and non-adherent cells were obtained two times from each patient with active pulmonary tuberculosis without any underlying diseases, on admission before the initiation of administering anti-tuberculous drugs and 2 months later from the negative conversion of Mycobacterium tuberculosis in sputum culture. ELISA was performed to measure IFN-gamma and IL-10 levels in culture media of adherent cells and non-adherent cells stimulated with PPD. IFN-gamma levels produced by non-adherent cells on admission were significantly higher than that of healthy controls (p < 0.001). Elevated IFN-gamma levels on admission was reduced after treatment for tuberculosis (p < 0.03), but still remained higher than that in healthy controls. IL-10 levels of non-adherent cells of patients were lower than those of healthy controls, although the difference was not significant. IL-10 levels produced by non-adherent cells on admission correlated with the time needed for negative conversion of bacilli in sputum culture (p < 0.05). IL-10 level produced by adherent cells from nutritionally normal patients were significantly higher than that of healthy controls (p < 0.05), and elevated IL-10 level was significantly reduced after therapy (p < 0.05). In the normonourished patients, the time needed for negative conversion of the bacilli in sputum culture of patients kept higher level of IL-10 of non-adherent cells (N = 5) was significantly longer than that of patients reduced IL-10 level after therapy. These results suggest that IL-10 produced by monocytes may diminish the TH1 responses of patients with pulmonary tuberculosis.
authors.affiliation: Second Department of Internal Medicine, Nara Medical University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakaya
authors.forename: T
authors.initials: T
authors.lastname: Yoneda
authors.forename: A
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Onohara
authors.forename: Y
authors.initials: Y
authors.lastname: Ikoma
authors.forename: A
authors.initials: A
authors.lastname: Fukuoka
authors.forename: K
authors.initials: K
authors.lastname: Tomoda
authors.forename: H
authors.initials: H
authors.lastname: Takenaka
authors.forename: H
authors.initials: H
authors.lastname: Okamura
authors.forename: C
authors.initials: C
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Fukuoka
authors.forename: T
authors.initials: T
authors.lastname: Tokuyama
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: M
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: K
authors.initials: K
authors.lastname: Tsukaguchi
authors.forename: N
authors.initials: N
authors.lastname: Narita
mesh_terms: D000208:Acute Disease; D000328:Adult; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014397:Tuberculosis, Pulmonary / Q000097:blood*
chemical_list: D016753:Interleukin-10; D007371:Interferon-gamma