title: Interferon-gamma-inducing factor gene transfection into Lewis lung carcinoma cells reduces tumorigenicity in vivo.
abstract: To investigate the immunoregulatory effect of murine interferon-gamma-inducing factor (mIGIF), we transfected Lewis lung carcinoma (LLC) cells with a mammalian expression vector containing the mIGIF complementary DNA. The culture medium of the transfectant cells stimulated interferon-gamma (IFN-gamma) production by spleen cells in vitro in the presence of anti-CD3 antibody and markedly potentiated the effect of interleukin-12 (IL-12) on IFN-gamma production by spleen cells. mIGIF transfectant cells showed reduction of tumorigenicity and induction of an in vivo immuno-protective effect against the parental LLC cells. To examine the combined effect of systemic administration of recombinant IL-12 (rIL-12) and local mIGIF on the tumorigenicity, mice were challenged with LLC or transfectant cells on day 0, and the tumor-bearing mice were injected with 50 ng of rIL-12 intraperitoneally from day 7 to 11. Systemic rIL-12 showed an anti-tumor effect. However, mIGIF gene expression did not potentiate this effect of systemic rIL-12 in vivo.
authors.affiliation: Pharmacology Division, National Cancer Center Research Institute, Tokyo.
authors.forename: H
authors.initials: H
authors.lastname: Fukumoto
authors.forename: M
authors.initials: M
authors.lastname: Nishio
authors.forename: K
authors.initials: K
authors.lastname: Nishio
authors.forename: Y
authors.initials: Y
authors.lastname: Heike
authors.forename: H
authors.initials: H
authors.lastname: Arioka
authors.forename: H
authors.initials: H
authors.lastname: Kurokawa
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: K
authors.initials: K
authors.lastname: Fukuoka
authors.forename: T
authors.initials: T
authors.lastname: Nomoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ohe
authors.forename: N
authors.initials: N
authors.lastname: Saijo
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000276:immunology* / Q000517:prevention & control; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics* / Q000276:immunology*; D018076:DNA, Complementary; D004347:Drug Interactions; D007114:Immunization; D007369:Interferon Inducers; D018664:Interleukin-12 / Q000276:immunology / Q000494:pharmacology; D020382:Interleukin-18; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000378:metabolism; D014162:Transfection
chemical_list: D016207:Cytokines; D018076:DNA, Complementary; D007369:Interferon Inducers; D020382:Interleukin-18; D011994:Recombinant Proteins; D018664:Interleukin-12