Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri.
Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.
Heng
N C
NC
Department of Microbiology, University of Otago, Dunedin, New Zealand.
Jenkinson
H F
HF
Tannock
G W
GW
D001407:Bacillus / Q000235:genetics*; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics; D044644:Endo-1,3(4)-beta-Glucanase; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism*; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping; D001616:beta-Galactosidase / Q000378:metabolism
D015342:DNA Probes; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; D044644:Endo-1,3(4)-beta-Glucanase