Comparison of the subcellular distribution of the messages encoding PLP and DM-20.
Oligonucleotides provide a means to differentiate between closely related mRNAs. Here in situ hybridization is performed using oligonucleotide probes to determine the subcellular distribution of two differential splicing products of a single gene. It is demonstrated here that the subcellular distributions of PLP and DM-20 messages in oligodendrocytes are quite different, despite being transcripts of a single gene.
McEwan
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NR
Department of Biochemistry, University of Stirling, Scotland.
D000818:Animals; D015342:DNA Probes; D017403:In Situ Hybridization; D018991:Myelin Proteolipid Protein / Q000235:genetics* / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D019154:Protein Splicing; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013347:Subcellular Fractions; D014158:Transcription, Genetic
D015342:DNA Probes; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C489071:Plp1 protein, rat; D012333:RNA, Messenger