title: ATR-FTIR spectroscopic investigation of E. coli transconjugants beta-lactams-resistance phenotype.
abstract: Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transconjugants TEM-1, TEM-2, TEM-3, SHV-2, SHV-3, SHV-4. A good discrimination of the susceptible strain from the transconjugants was obtained. Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM-2 and SHV-3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to beta-lactamase point-mutations.
authors.affiliation: Laboratoire de Spectroscopie Biomoléculaire, IFR 53, UFR de Pharmacie,Université de Reims Champagne-Ardenne, France.
authors.forename: W
authors.initials: W
authors.lastname: Bouhedja
authors.forename: G D
authors.initials: GD
authors.lastname: Sockalingum
authors.forename: P
authors.initials: P
authors.lastname: Pina
authors.forename: P
authors.initials: P
authors.lastname: Allouch
authors.forename: C
authors.initials: C
authors.lastname: Bloy
authors.forename: R
authors.initials: R
authors.lastname: Labia
authors.forename: J M
authors.initials: JM
authors.lastname: Millot
authors.forename: M
authors.initials: M
authors.lastname: Manfait
mesh_terms: D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D047090:beta-Lactams