Colocalization of parvalbumin, calretinin and calbindin D-28k in human cortical and subcortical visual structures.
Several studies have demonstrated that three calcium-binding proteins parvalbumin (PV), calbindin D-28k (CB) and calretinin (CR) mark distinct subsets of cortical interneurons. This study demonstrates, in cortical and subcortical visual structures, the coexistence of two calcium-binding proteins in some neuronal subpopulations. The human visual cortex (VC), lateral geniculate nucleus (LGN). lateral inferior pulvinar (LIP) and superior colliculus (SC) were examined by a double-labelling immunocytochemical technique. The VC showed mostly separate populations of PV, CB and CR immunoreactive (-ir) interneurons, but also small populations of double-stained PV + CR and CR + CB neurons, while PV + CB neurons were less frequent. An average of 2.5% of the immunoreactive neurons were double-stained for PV + CR and 7.1% for CR + CB in area 17, while this percentage was slightly higher in association area 18 (3.3 and 7.4%, respectively). In the LGN and LIP, double-stained neurons were scarce, but in the fibre capsule of these nuclei, as well as in the optic radiation (OR) and white matter underlying area 17, both double-stained PV + CR or CR + CB and separate populations of PV-ir, CB-ir and CR-ir neurons and fibres were observed. Unlike the thalamic regions, the SC showed some double-stained PV + CR and CR + CB neurons, scattered both in the superficial and deep layers. These findings are discussed in the light of similar observations recently reported from other regions of the human brain.
Leuba
G
G
University Psychogeriatrics Hospital, Lausanne-Prilly, Switzerland. Genevieve.Leuba@inst.hospvd.ch
Saini
K
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000918:Antibody Specificity; D064032:Calbindin 2; D064026:Calbindins; D005260:Female; D005829:Geniculate Bodies / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D007395:Interneurons / Q000737:chemistry / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D009412:Nerve Fibers / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000276:immunology; D010320:Parvalbumins / Q000032:analysis* / Q000276:immunology; D064030:S100 Calcium Binding Protein G / Q000032:analysis* / Q000276:immunology; D014793:Visual Cortex / Q000737:chemistry* / Q000166:cytology
C576764:CALB2 protein, human; D064032:Calbindin 2; D064026:Calbindins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G