title: Deposition of extracellular matrix on silicone intraocular lens implants in rabbits.
abstract: OBJECTIVE
  To examine the deposition of extracellular matrix on silicone intraocular lenses (IOLs) implanted experimentally into rabbit eyes by electron microscopy and to determine the immunolocalization of extracellular matrix components, including collagen types and cellular fibronectin, on these IOLs.
  
  
  METHODS
  We performed phacoemulsification and aspiration of the crystalline lens and implanted a foldable silicone IOL in the capsular bag of one eye of each of 26 adult albino rabbits under general anesthesia. After 8 weeks the animals were killed and the eyes were enucleated. The silicone IOLs were processed for electron microscopy and for immunohistochemical detection of collagen types I, III, and IV and cellular fibronectin.
  
  
  RESULTS
  Electron microscopy revealed deposition of a presumed cell matrix complex on the optic portion of all silicone IOLs, as well as the adhesion of presumed macrophages and foreign-body giant cells. Cellular deposits showed immunoreactivity for cellular fibronectin. Fibrous or membranous deposits exhibited immunoreactivity for cellular fibronectin and collagen types I and III. A few type IV collagen-immunoreactive deposits were also seen.
  
  
  CONCLUSIONS
  Deposits of extracellular matrix components were observed on silicone IOLs. These deposits may form the scaffolding for the adhesion and proliferation of cells. These matrix components appeared to be the products of cells adhering to the surfaces of IOLs, including lens epithelial cells, macrophages and foreign-body giant cells, indicating that the process of granulation was incomplete.
authors.affiliation: Department of Ophthalmology, Wakayama Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saika
authors.forename: S
authors.initials: S
authors.lastname: Ohmi
authors.forename: A
authors.initials: A
authors.lastname: Ooshima
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: A
authors.initials: A
authors.lastname: Yamanaka
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000000981:diagnostic imaging / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D007910:Lenses, Intraocular; D008297:Male; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D018918:Phacoemulsification; D011817:Rabbits; D012826:Silicone Elastomers; D000267:Tissue Adhesions; D014463:Ultrasonography
chemical_list: D005353:Fibronectins; D012826:Silicone Elastomers; D003094:Collagen