title: Suppressed formation of bone marrow adherent layers derived from acute myeloid leukemia patients after in vitro exposure to interleukin-4.
abstract: Long-term bone marrow cultures from ten acute myeloid leukemia (AML) patients exposed to recombinant human (rhu) interleukin (IL) 4 from culture initiation failed to develop adherent layers at 5 weeks as compared to controls. The adherent layers from two of our patients expressed IL-1 beta transcripts constitutively, and all produced IL-6 and leukemia inhibitory factor (LIF) proteins. Our results demonstrate that rhuIL-4 markedly inhibited AML-derived adherent layer formation in a time- and dose-dependent manner, and this effect was not mediated through enhanced apoptosis and did not correlate with IL-1 beta, IL-6 or LIF production.
authors.affiliation: Department of Hematologic Oncology, Roswell Park Cancer Institute, State University of New York at Buffalo, NY 14263, USA.
authors.forename: M
authors.initials: M
authors.lastname: Wetzler
authors.forename: R
authors.initials: R
authors.lastname: Kurzrock
authors.forename: Z
authors.initials: Z
authors.lastname: Estrov
authors.forename: S
authors.initials: S
authors.lastname: Barone
authors.forename: E
authors.initials: E
authors.lastname: Estey
authors.forename: M
authors.initials: M
authors.lastname: Talpaz
mesh_terms: D000208:Acute Disease; D017209:Apoptosis; D001853:Bone Marrow / Q000473:pathology*; D002448:Cell Adhesion; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D011994:Recombinant Proteins; D015847:Interleukin-4