The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells.
In recent years, fluorescence microscopy imaging has become an important tool for studying cell structure and function. This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting living cells. In this paper, we present results using various commercially available fluorescent probes as well as some developed in our laboratory and discuss the advantages and limitations of these probes in confocal microscopy studies of the cardiovascular system.
Bkaily
G
G
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
Pothier
P
P
D'Orléans-Juste
P
P
Simaan
M
M
Jacques
D
D
Jaalouk
D
D
Belzile
F
F
Hassan
G
G
Boutin
C
C
Haddad
G
G
Neugebauer
W
W
D000818:Animals; D001011:Aorta; D002118:Calcium / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D002642:Chick Embryo; D006224:Cricetinae; D003600:Cytosol / Q000737:chemistry; D004721:Endoplasmic Reticulum / Q000378:metabolism; D019332:Endothelin-1 / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D005333:Fetus; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D008929:Mitochondria, Heart / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D009206:Myocardium / Q000166:cytology*; D015388:Organelles / Q000378:metabolism; D010972:Platelet Activating Factor / Q000378:metabolism; D044022:Receptor, Endothelin A; D011945:Receptors, Angiotensin / Q000378:metabolism; D017466:Receptors, Endothelin / Q000378:metabolism
D019332:Endothelin-1; D005456:Fluorescent Dyes; D007375:Interleukin-1; D010972:Platelet Activating Factor; D044022:Receptor, Endothelin A; D011945:Receptors, Angiotensin; D017466:Receptors, Endothelin; D002118:Calcium