Analysis of the genome of an Epstein-Barr-virus (EBV)-related herpesvirus in a cynomolgus monkey cell line (Si-IIA).
A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV)-related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV.
Ino
H
H
Second Department of Pathology, Okayama University Medical School, Japan.
Hayashi
K
K
Yanai
H
H
Teramoto
N
N
Koirala
T R
TR
Chen
H L
HL
Oka
T
T
Yoshino
T
T
Takahashi
K
K
Akagi
T
T
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000737:chemistry*; D016679:Genome, Viral; D006568:Herpesvirus 1, Cercopithecine / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000662:veterinary / Q000821:virology*; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits
D004279:DNA, Viral