Effect of ciprofloxacin on the accumulation of interleukin-6, interleukin-8, and nitrite from a human endothelial cell model of sepsis.
OBJECTIVE
To determine the effect of the quinolone antibiotic ciprofloxacin, on interleukin-6, interleukin-8, and nitrite production by human endothelial cells.


METHODS
Controlled cell culture experiments examining the immunomodulatory effects of an antibiotic.


METHODS
University research laboratory attached to a large teaching hospital.


METHODS
A human endothelial cell line.


RESULTS
Cells were incubated with tumor necrosis factor-alpha and interleukin-1beta in the presence of a range of ciprofloxacin concentrations. Interleukin-6, interleukin-8, and nitrite concentrations were measured in culture supernatants after 24 hrs using enzyme immunoassay. Ciprofloxacin decreased interleukin-6 accumulation (p = .001). Interleukin-8 was decreased at lower ciprofloxacin concentrations (p = .017) but was increased at 100 microg/mL (p = .0039). Ciprofloxacin had no effect on nitrite accumulation (p = .38).


CONCLUSIONS
Ciprofloxacin differentially modulates interleukin-6 and interleukin-8 expression. The response to infection is coordinated by a cascade of cytokines and other mediators. The widespread use of ciprofloxacin in patients with severe infections is likely to result in alterations in local concentrations of cytokines. Selective control of cytokine concentrations by antibacterial agents will clearly have important therapeutic implications and may be a future research consideration in antibiotic drug design.
Galley
H F
HF
Academic Unit of Anaesthesia & Intensive Care, University of Aberdeen, Institute of Medical Sciences, UK.
Nelson
S J
SJ
Dubbels
A M
AM
Webster
N R
NR
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D002939:Ciprofloxacin / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis* / Q000378:metabolism; D016209:Interleukin-8 / Q000032:analysis* / Q000378:metabolism; D008175:Lung Neoplasms; D009573:Nitrites / Q000032:analysis*; D018805:Sepsis / Q000188:drug therapy* / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000890:Anti-Infective Agents; D015850:Interleukin-6; D016209:Interleukin-8; D009573:Nitrites; D002939:Ciprofloxacin