Application of terbium sensitized fluorescence for the determination of fluoroquinolone antibiotics pefloxacin, ciprofloxacin and norfloxacin in serum.
A simple, rapid and sensitive spectrofluorimetric method for the determination of fluoroquinolone antibiotics, norfloxacin (NOR), ciprofloxacin (CIP) and pefloxacin (PEF) is described. The method is based on the radiative energy transfer from fluoroquinolones to terbium ions (Tb3+) in the presence of tri-n-octylphosphine oxide (TOPO) in weakly acidic (pH 5.5) micellar solution of cetylpyridinium chloride (CPCI). Optimum conditions for the formation of the fluoroquinolone-Tb(3+)-TOPO ternary complexes have been investigated. Under optimized conditions the detection limits are 1.7, 1.2 and 4.4 nM for NOR, CIP and PEF, respectively, while the range of application for all three drugs is 0.05-10 microM. The method has been successfully applied to the determination of NOR, CIP and PEF in serum samples after deproteinization with acetonitrile (serum-acetonitrile; 1:2, v/v). The mean recoveries from serum samples spiked with NOR, CIP and PEF (5.0-50.0 microM) were (90.3 +/- 4.9), (105.0 +/- 3.6), and (95.3 +/- 1.5)% respectively. Within-run and day-to-day s, values for 5.0, 25.0 and 50.0 microM of each fluoroquinolone varied from 1.7 to 5.4% and from 3.3 to 12.8%, respectively. The influence of several usually coadministered drugs on the determination of fluoroquinolones in serum has been investigated.
Veiopoulou
C J
CJ
University of Athens, Laboratory of Analytical Chemistry, Greece.
Ioannou
P C
PC
Lianidou
E S
ES
D000890:Anti-Infective Agents / Q000097:blood*; D002594:Cetylpyridinium / Q000737:chemistry; D002939:Ciprofloxacin / Q000097:blood; D005453:Fluorescence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009643:Norfloxacin / Q000097:blood; D009943:Organophosphorus Compounds / Q000737:chemistry; D015366:Pefloxacin / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000737:chemistry*; D013997:Time Factors
D000890:Anti-Infective Agents; D009943:Organophosphorus Compounds; D013725:Terbium; D015366:Pefloxacin; D002939:Ciprofloxacin; C044965:trioctyl phosphine oxide; D002594:Cetylpyridinium; D009643:Norfloxacin