title: Loss of telomerase activity during male germ cell differentiation.
abstract: Although the activity of telomerase, an enzyme which synthesizes telomeres de novo and stabilizes telomere length has been demonstrated in the testis, the precise expression of activity in different germ cell types is not known. We examined telomerase activity using a PCR-based telomeric repeat amplification protocol during development of the rat testis from birth to adulthood. Telomerase activity was relatively high from birth to the 4th week of age, and then low between the 5th to 10th week, suggesting that the type A spermatogonial stem cells may be the population which is expressing the highest levels of telomerase activity. To ascertain which germ cells expresses the telomerase activity, purified populations of type A spermatogonia from 9-day old rats, and pachytene spermatocytes, round spermatids and epididymal spermatozoa from adult rats were isolated. While type A spermatogonia expressed very strong telomerase activity, the fractions containing pachytene spermatocytes and round spermatids also expressed telomerase activity, but at comparatively lower levels. Telomerase activity was totally absent in epididymal spermatozoa. Thus, it appears that the telomerase activity is expressed at high levels in the type A spermatogonial stem cells, is down-regulated during spermatogenesis, and is absent in the differentiated spermatozoa.
authors.affiliation: Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA.
authors.forename: N
authors.initials: N
authors.lastname: Ravindranath
authors.forename: R
authors.initials: R
authors.lastname: Dalal
authors.forename: B
authors.initials: B
authors.lastname: Solomon
authors.forename: D
authors.initials: D
authors.lastname: Djakiew
authors.forename: M
authors.initials: M
authors.lastname: Dym
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013087:Spermatids / Q000201:enzymology; D013090:Spermatocytes / Q000201:enzymology; D013093:Spermatogonia / Q000201:enzymology; D013094:Spermatozoa / Q000166:cytology* / Q000201:enzymology*; D019098:Telomerase / Q000378:metabolism*; D013737:Testis / Q000201:enzymology / Q000254:growth & development
chemical_list: D019098:Telomerase