Comparison of immune responses in patients infected with Vibrio cholerae O139 and O1.
Vibrio cholerae O139 has recently emerged as the second etiologic agent of cholera in Asia. A study was carried out to evaluate the induction of specific immune responses to the organism in V. cholerae O139-infected patients. The immune responses to V. cholerae O139 Bengal were studied in patients by measuring antibody-secreting cells (ASC), as well as vibriocidal and antitoxic antibodies in the circulation. These responses were compared with those in patients with V. cholerae O1 disease. Strong immunoglobulin A (IgA) and IgM ASC responses were seen against the homologous lipopolysaccharide or serogroup of V. cholerae. The magnitude and isotype of the responses were similar in O139- and O1-infected patients. Vibriocidal antibody responses were seen against bacteria of the homologous but not heterologous serogroup, and these responses reflect the lack of cross-protection between the infections caused by the two serogroups. The two groups of patients showed comparable cholera toxin-specific ASC responses, with the IgG isotype dominating over the IgA isotype, as well as comparable antitoxic immune responses in plasma. These results suggest that despite having a polysaccharide capsule, V. cholerae O139 induces systemic and intestine-derived ASC responses in peripheral blood comparable to those seen in patients with V. cholerae O1 disease.
Qadri
F
F
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka. fqadri@cholera.bangla.net
Wennerås
C
C
Albert
M J
MJ
Hossain
J
J
Mannoor
K
K
Begum
Y A
YA
Mohi
G
G
Salam
M A
MA
Sack
R B
RB
Svennerholm
A M
AM
D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology; D002771:Cholera / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology; D003429:Cross Reactions; D003967:Diarrhea / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology*
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D002772:Cholera Toxin