Fetal and adult hair follicle. An immunohistochemical study of anticytokeratin antibodies in formalin-fixed and paraffin-embedded tissue.
The expression of cytokeratins (CK), involucrin, vimentin, CD34, and alpha-smooth-muscle actin was studied in fetal and adult hair follicles. The first stage of the developing hair follicle is characterized by palisaded, elongated epithelial cells budding from the epidermal basal layer. These cells express CK5/6, CK14, CK17, CK19, and vimentin. During the following weeks of gestation, different structures in the developing hair follicle can be identified and characterized. The matrical cells display only CK19. The keratinocytes of the outer root sheath express CK5/6, CK14, CK17, CK19, and involucrin; those of the inner root sheath, CK4, CK18, and involucrin; those of the isthmus, the same profile as the ORS. In the infundibulum, the basal-layer keratinocytes express CK5/6, CK14, CK17, and CK19, whereas in the suprabasal layers CK1, CK4, CK10, CK14, and CK17 are seen. The adult hair follicle in anagen fails to express CK19 in the matrical cells and isthmus and both CK17 and CK19 in the infundibulum. These profiles of intermediate filaments and other markers appear to be potentially useful in categorizing neoplasms with apparent follicular differentiation.
Schirren
C G
CG
Department of Dermatology Ludwig-Maximilians-University, Munich, Germany.
Burgdorf
W H
WH
Sander
C A
CA
Plewig
G
G
D000199:Actins / Q000032:analysis; D000328:Adult; D000906:Antibodies; D018952:Antigens, CD34 / Q000032:analysis; D004817:Epidermis / Q000033:anatomy & histology / Q000196:embryology; D004848:Epithelium / Q000033:anatomy & histology / Q000196:embryology; D005333:Fetus; D005404:Fixatives; D005557:Formaldehyde; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D018859:Hair Follicle / Q000033:anatomy & histology* / Q000196:embryology; D006801:Humans; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000648:ultrastructure; D015603:Keratinocytes / Q000166:cytology; D007633:Keratins / Q000032:analysis* / Q000235:genetics; D016612:Paraffin Embedding; D011498:Protein Precursors / Q000032:analysis; D016707:Tissue Fixation; D014746:Vimentin / Q000032:analysis
D000199:Actins; D000906:Antibodies; D018952:Antigens, CD34; D005404:Fixatives; D011498:Protein Precursors; D014746:Vimentin; D005557:Formaldehyde; C032253:involucrin; D007633:Keratins