title: A thermal denaturation study of macronuclear chromatin in Blepharisma japonicum (Protozoa, Ciliophora, Heterotrichida).
abstract: The macronuclear chromatin of the ciliate Blepharisma japonicum, in two starvation states, was studied by thermal denaturation analysis. The behaviour of B. japonicum chromatin, native and reconstituted with Tetrahymena pyriformis H1 histone, was analysed. The data obtained are consistent with the hypothesis that B. japonicum macronuclear chromatin contains a H1-like peptide associated with the linker DNA, although this peptide is reduced in amount and/or chromatin stabilising ability when compared to Tetrahymena macronuclear H1.
authors.affiliation: Scuola Normale Superiore di Pisa, Italy. m.salvini@discat.unipi.it
authors.forename: M
authors.initials: M
authors.lastname: Salvini
authors.forename: T
authors.initials: T
authors.lastname: Dalle Lucche
authors.forename: M
authors.initials: M
authors.lastname: Durante
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry*; D002843:Chromatin / Q000737:chemistry*; D016798:Ciliophora / Q000737:chemistry*; D016054:DNA, Protozoan / Q000737:chemistry; D006657:Histones / Q000737:chemistry; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D011489:Protein Denaturation; D013769:Tetrahymena pyriformis / Q000737:chemistry
chemical_list: D002843:Chromatin; D016054:DNA, Protozoan; D006657:Histones