[Cloning of ribR, an additional regulatory gene of the Bacillus subtilis riboflavin operon].
A 13.0-kb EcoRI fragment of Bacillus subtilis DNA carrying an additional regulatory ribR gene of riboflavin operon was cloned on the basis of resistance to 7, 8-dimethyl-10 (O-methylacetoxym)-isoalloxasin. The cloned fragment was trans-dominant with regard to ribC constitutive mutations that block the overproduction of riboflavin but inactive relative to constitutive mutations in the rib(O) regulatory region.
Solov'eva
I M
IM
Iomantas
Iu A
IuA
Kreneva
R A
RA
Kozlov
Iu I
IuI
Perumov
D A
DA
D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D004352:Drug Resistance, Microbial; D005415:Flavins / Q000494:pharmacology; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009876:Operon; D012256:Riboflavin / Q000235:genetics*
C107664:7,8-dimethyl-10-(O-methylacetoxy)isoalloxasin; D004269:DNA, Bacterial; D005415:Flavins; D015246:Deoxyribonuclease EcoRI; D012256:Riboflavin