Trophic action of pharmacological substances with a guanidine group on mouse neuroblastoma cells and chick ganglionic neurons in culture.
A series of substances (designated CTQ compounds) with a guanidine group have been synthesized and tested for their ability to promote neuronal survival and neurite outgrowth. Mouse neuroblastoma clonal cell lines grown in serum-containing medium for 10 days as well as primary cultures of embryonic chicken ganglion neurons grown in serum-free defined medium for 1 or 2 days have been used for the experiments. Among the various CTQ compounds (CTQ1-CTQ20) tested, only CTQ8 exerted positive neurotrophic effects on these peripheral neuronal cells. At a concentration of 10(-4) M, CTQ8 enhanced neuritogenesis of neuroblastoma cells. However, the most striking influence of CTQ8 was its promoting effect (6- to 10-fold) on the survival of chicken ciliary and dorsal root ganglionic neurons at concentrations ranging from 10(-3) M to 5 x 10(-4) M.
Pettmann
B
B
Laboratoire de Neurobiologie Ontogénique, ER588 CNRS, Centre de Neurochimie du CNRS, Strasbourg, France.
Escurat
M
M
Quirosa-Guillou
C
C
Thal
C
C
Portier
M M
MM
Sensenbrenner
M
M
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002924:Ciliary Body / Q000166:cytology; D005727:Ganglia, Spinal / Q000166:cytology; D006146:Guanidines / Q000138:chemical synthesis / Q000494:pharmacology*; D051379:Mice; D016501:Neurites / Q000502:physiology; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D011743:Pyrimidines / Q000494:pharmacology
D006146:Guanidines; D011743:Pyrimidines; C020375:isaxonine