title: Cellular eukaryotic initiation factor 5A content as a mediator of polyamine effects on growth and apoptosis.
abstract: The polyamines are essential for eukaryotic cell growth. One of the most critical effects of polyamines on cell growth is the availability of spermidine for the post-translational modification of eIF-5A. Because hypusine-containing eIF-5A is necessary for cell proliferation, depletion of cellular polyamines suppresses growth by depleting cellular modified eIF-5A content. Excess putrescine accumulations in DH23A/b cells induces apoptosis and suppresses the formation of hypusine-containing eIF-5A. Treatment of DH23A/b cells with diaminoheptane also suppresses modified eIF-5A formation and induces apoptosis. These data suggest that suppression of modified eIF-5A formation may play a role in putrescine-induced apoptosis as well.
authors.affiliation: Department of Biochemistry, University of Arizona, Tucson 85724, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Tome
authors.forename: E W
authors.initials: EW
authors.lastname: Gerner
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002455:Cell Division; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000502:physiology; D010448:Peptide Initiation Factors / Q000502:physiology*; D011700:Putrescine / Q000502:physiology*; D016601:RNA-Binding Proteins; D015398:Signal Transduction; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D008239:Lysine; D010448:Peptide Initiation Factors; D011700:Putrescine; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C100028:hypusine