title: Retroviral particles produced from a stable human-derived packaging cell line transduce target cells with very high efficiencies.
abstract: The goal of this work was to determine whether a stable 293 amphotropic packaging line, which we have designated 293-SPA, is useful for the production of high-titer stable virus by comparison to the murine psiCRIP line. Here, we report our unexpected findings that particles derived from the 293-SPA line transduce target cells (both NIH-3T3 cells and primary melanoma cells) with greatly enhanced efficiencies (at least 10-fold) compared to particles derived from the psiCRIP packaging line. We show that the presence of a transferable inhibitor in the psiCRIP line at least partially accounts for this dramatic difference in transduction efficiency. This work has important implications for improving the efficiency of retrovirus-mediated gene transfer in general as well as in the design of new packaging cell lines.
authors.affiliation: Somatix Therapy Corporation, Alameda, CA 94010, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Davis
authors.forename: R M
authors.initials: RM
authors.lastname: Witt
authors.forename: P R
authors.initials: PR
authors.lastname: Gross
authors.forename: C A
authors.initials: CA
authors.lastname: Hokanson
authors.forename: S
authors.initials: S
authors.lastname: Jungles
authors.forename: L K
authors.initials: LK
authors.lastname: Cohen
authors.forename: O
authors.initials: O
authors.lastname: Danos
authors.forename: S K
authors.initials: SK
authors.lastname: Spratt
mesh_terms: D016475:3T3 Cells / Q000821:virology; D000818:Animals; D002460:Cell Line; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000196:embryology / Q000821:virology*; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000821:virology; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D001616:beta-Galactosidase