Percutaneous implantation of non-small-cell lung carcinoma: technique and observations.
OBJECTIVE
The authors performed this study to determine whether intrathoracic inoculation of non-small-cell lung carcinoma with fluoroscopic guidance would provide for more accurate implantation.


METHODS
A tumor cell inoculum (2 x 10(6) cells per 0.15 mL) was injected percutaneously under fluoroscopic guidance at the posterior midaxillary line in 22 athymic nude mice. The mice underwent imaging with a mammographic unit at 3, 5, and 8 weeks after implantation. The mice were sacrificed at 8 weeks, and autopsy was performed to determine tumor yield.


RESULTS
The use of a percutaneous technique under fluoroscopic guidance greatly facilitated the accurate implantation of xenografts. Tumor growth was seen at radiography in 18 of the 22 (82%) mice at 8 weeks. Necropsy revealed a 100% tumor yield. Histologic examination confirmed adenocarcinoma of the lung. The average number of tumors found in the lung parenchyma was 1.05 +/- 0.35; the average number of tumors found in the mediastinum was 0.59 +/- 0.67. The average tumor weight was 389 mg +/- 64.3. The average tumor size was 300 mm3 +/- 66.23.


CONCLUSIONS
With fluoroscopic guidance, percutaneous implantation of tumor cells in athymic nude mice is simple and effective.
Hamide
J P
JP
Department of Radiology, Louisiana State University Medical Center, New Orleans 70112, USA.
Qian
Z
Z
Xu
H
H
Diethelm
L
L
Skrepnik
N
N
Castaneda-Zuniga
W R
WR
Hunt
J D
JD
D000230:Adenocarcinoma / Q000000981:diagnostic imaging* / Q000473:pathology; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000000981:diagnostic imaging* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005471:Fluoroscopy / Q000379:methods*; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured