Characterization of the myosin heavy chains of avian adult fast muscles at the protein and mRNA levels.
High resolution anion-exchange chromatography of myosin subfragment-1 in avian fast muscles revealed five fast heavy chains (I-V) expressed in muscle-specific patterns. Sequence analysis of a unique peptide established that the proteins differed in primary structure and suggested correlation with heavy chain genes identified independently by Robbins and coworkers. The identities of the isoforms and their expression patterns were confirmed at the mRNA level by a reverse-transcription, 5'-anchored PCR procedure. The fast white pectoralis major muscle possessed heavy chain I, the posterior latissimus dorsi muscle, of similar fibre type, expressed heavy chains I, III and IV. The fast red adductor superficialis muscle expressed either, or both, of heavy chains II and IV. The lateral gastocnemius muscle, of mixed fibre type, expressed heavy chains II-V. In general, heavy chains I, III and V appeared to be favoured in fast white fibres, while heavy chains II and IV were characteristic of fast red fibres. These results imply a greater subtlety of fast muscle function than has previously been appreciated.
Rushbrook
J I
JI
Department of Biochemistry, State University of New York Health Science Center at Brooklyn 11203, USA.
Huang
J
J
Weiss
C
C
Siconolfi-Baez
L
L
Yao
T T
TT
Becker
E
E
Feuerman
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002852:Chromatography, Ion Exchange; D015870:Gene Expression / Q000502:physiology; D007527:Isoenzymes / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry / Q000502:physiology*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology*; D018995:Myosin Heavy Chains / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis
D007527:Isoenzymes; D012333:RNA, Messenger; D018995:Myosin Heavy Chains