title: Radioiodinated antibody targeting of the HER-2/neu oncoprotein.
abstract: The HER-2/neu oncogene encodes a 185 kDa phosphoglycoprotein that is overexpressed in breast, ovarian and other cancers. Seven monoclonal antibodies reactive with oncoprotein were labeled with 131I. In vitro experiments with SKOv3 9002-18 cells determined binding affinity, internalization and degradation. The biodistribution of these antibodies in comparison to 125I-labeled nonspecific antibody was measured in athymic mice with SKOv3 9002-18 ovarian carcinoma xenografts. Antibody 520C9 exhibited the highest and most specific retention in tumor, peaking at 17.4 +/- 5.6% ID/g at 24 h.
authors.affiliation: Division of Medicine, University of Texas M.D. Anderson Cancer Center Houston 77030, USA.
authors.forename: F J
authors.initials: FJ
authors.lastname: Xu
authors.forename: Y H
authors.initials: YH
authors.lastname: Yu
authors.forename: D S
authors.initials: DS
authors.lastname: Bae
authors.forename: X G
authors.initials: XG
authors.lastname: Zhao
authors.forename: S K
authors.initials: SK
authors.lastname: Slade
authors.forename: C M
authors.initials: CM
authors.lastname: Boyer
authors.forename: R C
authors.initials: RC
authors.lastname: Bast
authors.forename: M R
authors.initials: MR
authors.lastname: Zalutsky
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics / Q000627:therapeutic use*; D000939:Epitopes; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016499:Radioimmunotherapy; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007457:Iodine Radioisotopes; D018719:Erb-b2 Receptor Tyrosine Kinases