title: Natural occurrence of fast- and fast/slow-muscle chimeric fibers in the expression of troponin T isoforms.
abstract: Rhomboideus, one of the back muscle tissues, and its single fibers were studied in chickens by immunostaining with antisera against fast- and slow-muscle-type troponin T isoforms. Nonuniform distribution of slow-muscle-type isoforms was for the first time detected in single fibers isolated from the muscle, although fast-muscle-type troponin T isoforms were distributed over the whole length of the fiber. Based on these observations, we conclude that fast- and fast/slow-muscle chimeric fibers exist in normal skeletal muscle tissue and that the existence of chimeric fibers is direct evidence showing that myonuclei subjected to different determination in troponin T isoform expression can together form a single muscle fiber.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakada
authors.forename: J I
authors.initials: JI
authors.lastname: Miyazaki
authors.forename: R
authors.initials: R
authors.lastname: Saba
authors.forename: T
authors.initials: T
authors.lastname: Hirabayashi
mesh_terms: D000375:Aging; D000818:Animals; D002645:Chickens; D002678:Chimera; D007150:Immunohistochemistry; D018656:Muscle Fibers, Fast-Twitch / Q000166:cytology / Q000378:metabolism*; D018657:Muscle Fibers, Slow-Twitch / Q000166:cytology / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D014336:Troponin / Q000096:biosynthesis*; D020107:Troponin T
chemical_list: D014336:Troponin; D020107:Troponin T