The influence of Pb2+ on expression of acetylcholinesterase and the acetylcholine receptor.
This paper examines the influence of inorganic lead (Pb2+) on the presence of acetylcholinesterase (AchE) molecular forms and the acetylcholine receptor (AchR) in two types of excitable tissue, primary cultures of skeletal muscle and neural retina from embryonic chick. Treatment of skeletal muscle with Pb2+ is observed to cause reductions in the 5/7S and 19S but not the 11.4S molecular forms of AchE. The reductions are dose-dependent, requiring submicromolar concentrations, slow in onset, requiring incubation times greater than 24 hr, and tissue specific, being pronounced in skeletal muscle but absent from neural retina. Significantly, the reductions in AchE occur without corresponding reductions in amounts of AchR and without reduction in activity of protein kinase C (PKC). These studies illustrate a tissue-specific action of inorganic lead that is not mediated through PKC.
Luo
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ZD
Department of Biochemical Pharmacology, State University of New York at Buffalo, 14260, USA.
Berman
H A
HA
D000110:Acetylcholinesterase / Q000096:biosynthesis* / Q000187:drug effects*; D000818:Animals; D002413:Cations, Divalent / Q000633:toxicity; D002478:Cells, Cultured; D002642:Chick Embryo; D007527:Isoenzymes / Q000187:drug effects / Q000378:metabolism; D007854:Lead / Q000633:toxicity*; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000096:biosynthesis* / Q000187:drug effects*; D011978:Receptors, Nicotinic / Q000096:biosynthesis / Q000187:drug effects; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D002413:Cations, Divalent; D007527:Isoenzymes; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D007854:Lead; D011493:Protein Kinase C; D000110:Acetylcholinesterase