title: Effect of interleukin-4 on allergen-induced arachidonic acid metabolism of rat peritoneal macrophages during immediate hypersensitivity reactions.
abstract: The aim of this study was to investigate the [3H]arachidonic acid metabolism of rat peritoneal macrophages, induced by allergen (ovalbumin) and the impact of interleukin-4 on this process. We established that ovalbumin induces an increase of [3H]arachidonic acid mobilisation from membrane lipids and of [3H]arachidonic acid catabolism, principally by the 5-lipoxygenase pathway, when the macrophages are sensitized and when serum is present. The allergen effect is not modified by the presence of interleukin-4 in the culture medium of macrophages 15 h before the allergen challenge. We also showed that, whereas the basal [3H]arachidonic acid metabolism of macrophages from control and actively sensitized rats is not different, interleukin-4 increases the [3H]arachidonic acid mobilisation and catabolism by cyclooxygenase and 5-lipoxygenase pathways in macrophages from control rats although it does not in macrophages from actively sensitized rats. In macrophages from control rats, the interleukin-4 effect is diminished by the addition of IgEs to their culture medium. In summary, interleukin-4 has an enhancer effect on the macrophage arachidonic acid catabolism that depends on the sensitization condition of the cell but that has no consequences on the further increased arachidonic acid metabolism induced by the allergen.
authors.affiliation: Laboratoire de la Signalisation et de la Différenciation des Macrophages, INSERM CJF 9107, Hôpital de Rangueil, Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: M'Rini
authors.forename: L
authors.initials: L
authors.lastname: Escoubet
authors.forename: A
authors.initials: A
authors.lastname: Rey
authors.forename: M
authors.initials: M
authors.lastname: Béraud
authors.forename: Y
authors.initials: Y
authors.lastname: Lamboeuf
authors.forename: M H
authors.initials: MH
authors.lastname: Séguelas
authors.lastname: Besombes
authors.forename: B
authors.initials: B
authors.lastname: Pipy
mesh_terms: D000485:Allergens; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D006969:Hypersensitivity, Immediate / Q000378:metabolism*; D007073:Immunoglobulin E / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D010047:Ovalbumin; D051381:Rats; D015854:Up-Regulation
chemical_list: D000485:Allergens; D015847:Interleukin-4; D016718:Arachidonic Acid; D007073:Immunoglobulin E; D010047:Ovalbumin