FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes.
The ability to place a series of gene constructs at a specific site in the genome opens new possibilities for the experimental examination of gene expression and chromosomal position effects. We report that the FLP- FRT site-specific recombination system of the yeast 2mu plasmid can be used to integrate DNA at a chromosomal FRT target site in Drosophila. The technique we used was to first integrate an FRT- flanked gene by standard P element-mediated transformation. FLP was then used to excise the FRT- flanked donor DNA and screen for FLP-mediated re-integration at an FRT target at a different chromosome location. Such events were recovered from up to 5% of the crosses used to screen for mobilization and are easily detectable by altered linkage of a white reporter gene or by the generation of a white + gene upon integration.
Golic
M M
MM
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA. golic@bioscience.utah.edu
Rong
Y S
YS
Petersen
R B
RB
Lindquist
S L
SL
Golic
K G
KG
D000818:Animals; D004247:DNA / Q000235:genetics*; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D005786:Gene Expression Regulation; D018390:Gene Targeting; D017344:Genes, Insect; D011995:Recombination, Genetic
D004247:DNA; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase