Calretinin is transiently expressed in tendon fibroblasts of the paravertebral region of the chick embryo.
Although calretinin is an intracellular calcium acceptor protein essentially located in neurons, we have previously shown that calretinin was also expressed in the mesenchymal cells forming the intervertebral disc. Here, we describe, using immunohistochemistry, the transient expression of calretinin in fibroblasts responsible for the tendon formation in the paravertebral region. We have looked at chick embryos from embryonic day 4 to day 18. At embryonic day 6, calretinin immunoreactivity was detected in chick embryo cells located laterally to the spinal cord between two groups of developing muscular cells. At embryonic day 8, calretinin immunoreactivity intensity was the highest in cells showing a fibroblast morphology. After embryonic day 8, when fibroblast proliferation decreased and differentiation increased, calretinin immunoreactivity progressively disappeared. Interestingly, calretinin could not be detected in fibroblasts of the anterior and posterior limbs at any investigated age. Because calretinin expression appeared selectively and transiently in the fibroblasts of the paravertebral region, we may conclude that the phenotype of those fibroblasts is different from the limbs fibroblasts.
Gangji
V
V
Laboratoire d'Histologie, Faculté de Médecine, Université Libre de Bruxelles, Belgium.
Krebs
C
C
Rooze
M
M
Pochet
R
R
D000818:Animals; D064032:Calbindin 2; D002642:Chick Embryo; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D007150:Immunohistochemistry; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis* / Q000737:chemistry; D013194:Staining and Labeling; D013710:Tendons / Q000737:chemistry / Q000196:embryology / Q000378:metabolism*; D013904:Thoracic Vertebrae
D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G