[Application of porous bioceramics for a hybrid type artificial liver system].
Biocompatible porous ceramic carrier was applied for culture of rat hepatocytes. Using composit sinters of hydroxyapatite (HAP) and beta-tricalcium phosphate (TCP) as culture carriers, freshly harvested rat hepatocytes were cultured. Adhesiveness of fibroblast L-929 cells on various TCP-HAP carriers showed that the composition of 20%-TCP and 80%-HAP is the most preferable for supporting tissue cultures. Hepatocyte function was evaluated by production rates of albumin and urea and consumption rate of glucose. The time-dependent variations of the hepatocytes functions suggest that the porous TCP-HAP carrier was effective for supporting three dimensional tissue structure.
Suzuki
T
T
Department of Biological Science and Technology, Science University of Tokyo.
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D002478:Cells, Cultured; D002516:Ceramics; D005947:Glucose / Q000378:metabolism; D006882:Hydroxyapatites; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D019164:Liver, Artificial; D016062:Porosity; D051381:Rats; D014508:Urea / Q000378:metabolism
D000418:Albumins; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006882:Hydroxyapatites; C485817:beta-tricalcium phosphate; D014508:Urea; D005947:Glucose; C018392:tricalcium phosphate