Molecular cloning of a novel receptor-type protein tyrosine phosphatase from murine fetal liver.
A cDNA fragment encoding a novel tyrosine phosphatase (PTPase), termed ptpf, was isolated from day 11.5 mouse fetal liver using reverse transcription-polymerase chain reaction (RT-PCR) with degenerate primers. The 5.5-kb cDNA encoding the complete coding region was isolated from an adult mouse kidney cDNA library. This cDNA contained a single open reading frame (ORF) encoding a predicted 1436-amino-acid protein with a molecular mass of 161,150 Da. Sequence analysis revealed that PTPf was homologous to PTPmu and PTPkappa, and a putative receptor-type PTPase. Northern blotting analysis of adult mouse mRNA indicated the existence of four major ptpf transcripts of approximately 10, 6, 3 and 2.7 kb, and these transcripts were expressed in a tissue-specific manner. During embryogenesis, only the 6-kb transcript was detected.
Yoneya
T
T
Pharmaceutical Research Laboratory, Kirin Brewery Co. Ltd, Gunma, Japan.
Yamada
Y
Y
Kakeda
M
M
Osawa
M
M
Arai
E
E
Hayashi
K
K
Nishi
N
N
Inoue
H
H
Nishikawa
M
M
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D011956:Receptors, Cell Surface; D017027:Protein Tyrosine Phosphatases