Modulation of lipid unsaturation and membrane fluid state in mammalian cells by stable transformation with the delta9-desaturase gene of Saccharomyces cerevisiae.
The composition and physical state of membrane lipids determine the dynamic nature of membranes, which in turn, could directly be linked to the activity of various membrane-associated cellular functions. To better understand the molecular basis of different membrane-related phenomena we established a novel strategy to alter unsaturation of mammalian cell membranes with an identical genetic background. We transfected L929 mouse fibroblastoid cells with DNA constructs containing the Delta9-fatty acid desaturase gene (Ole1) of S. cerevisiae under the control of desaturase promoters derived either from wild type or mutant strains of the dimorphic fungus H. capsulatum.
Gyorfy
Z
Z
Institute of Biochemistry, BRC, Szeged, Hungary.
Horváth
I
I
Balogh
G
G
Domonkos
A
A
Duda
E
E
Maresca
B
B
Vígh
L
L
D000818:Animals; D002461:Cell Line, Transformed; D004271:DNA, Fungal; D044943:Fatty Acid Desaturases / Q000235:genetics*; D050356:Lipid Metabolism; D008560:Membrane Fluidity; D051379:Mice; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013230:Stearoyl-CoA Desaturase; D014170:Transformation, Genetic
D004271:DNA, Fungal; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase