Strongyloides stercoralis: identification of antigens in natural human infections from endemic areas of the United States.
Using Western-blot analysis, we identified eight immunodominant antigens (apparent molecular weights 96, 86, 75, 56, 41, 32, 28, and 26 kDa) of Strongyloides stercoralis in natural human infections. For this study, 78 individual serum samples were obtained from S. stercoralis-infected patients residing in endemic areas of the United States. Poly A+ RNA was translated in vitro in the rabbit-reticulocyte lysate system. The newly synthesized translation products were immuno-precipitated with S. stercoralis human infection sera. All eight of the identified antigens were detected in the immunoprecipitates. The potential of these antigens as targets for immunodiagnosis is also discussed.
Siddiqui
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AA
Department of Biochemistry and Molecular Biology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614, USA. siddiqui@access.etsu-tn.edu
Koenig
N M
NM
Sinensky
M
M
Berk
S L
SL
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D015153:Blotting, Western; D004285:Dogs; D015801:Helminth Proteins / Q000276:immunology; D006801:Humans; D008970:Molecular Weight; D017171:Strongyloides stercoralis / Q000276:immunology*; D013322:Strongyloidiasis / Q000276:immunology*; D014481:United States
D000947:Antigens, Helminth; D015801:Helminth Proteins