Structure and expression of human fibroblast growth factor-10.
We isolated the cDNA encoding a novel member of the human fibroblast growth factor (FGF) family from the lung. The cDNA encodes a protein of 208 amino acids with high sequence homology (95.6%) to rat FGF-10, indicating that the protein is human FGF-10. Human FGF-10 as well as rat FGF-10 has a hydrophobic amino terminus ( approximately 40 amino acids), which may serve as a signal sequence. The apparent evolutionary relationships of human FGFs indicate that FGF-10 is closest to FGF-7. Chromosomal localization of the human FGF-10 gene was examined by in situ hybridization. The gene was found to map to the 5p12-p13 region. Human FGF-10 (amino acids 40 to 208 with a methionine residue at the amino terminus) was produced in Escherichia coli and purified from the cell lysate. Recombinant human FGF-10 (approximately 19 kDa) showed mitogenic activity for fetal rat keratinizing epidermal cells, but essentially no activity for NIH/3T3 cells, fibroblasts. The specificity of mitogenic activity of FGF-10 is similar to that of FGF-7 but distinct from that of bFGF. In structure and biological activity, FGF-10 is similar to FGF-7.
Emoto
H
H
Department of Genetic Biochemistry, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto 606-01, Japan.
Tagashira
S
S
Mattei
M G
MG
Yamasaki
M
M
Hashimoto
G
G
Katsumata
T
T
Negoro
T
T
Nakatsuka
M
M
Birnbaum
D
D
Coulier
F
F
Itoh
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D019143:Evolution, Molecular; D051526:Fibroblast Growth Factor 10; D005346:Fibroblast Growth Factors / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D017403:In Situ Hybridization; D008168:Lung / Q000737:chemistry; D008934:Mitogens / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
C496463:FGF10 protein, human; D051526:Fibroblast Growth Factor 10; D008934:Mitogens; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors