Production of human antibody repertoires in transgenic mice.
Transgenic mice have been created that carry human immunoglobulin heavy and light chain genes in germline configuration and that have the corresponding endogenous genes silenced. The transgenes are either minigene constructs or large, almost authentic, transloci on yeast artificial chromosomes and undergo B-cell-specific DNA rearrangement and hypermutation in the mouse lymphoid tissue. Monoclonal antibodies with good affinities for human antigens have been obtained after immunisation. These mice may be a future source of human antibodies for therapy.
Brüggemann
M
M
Laboratory of Developmental Immunology and Translocus Therapeutics, Ltd., Babraham Institute, Cambridge, UK. Marianne.Bruggemann@bbsrc.ac.uk
Taussig
M J
MJ
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D018244:Chromosomes, Artificial, Yeast; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis; D051379:Mice; D008822:Mice, Transgenic; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D011994:Recombinant Proteins