The stability of truncated epidermal growth factor receptor mRNA is higher than that of the full-length receptor mRNA in rat hepatoma cells.
Truncated and full-length epidermal growth factor (EGF) receptors are produced in rat liver cells. The truncated EGF receptor mRNA is almost identical to the full-length EGF receptor mRNA except for the lack of a 3' region of the full-length receptor mRNA. To understand the stability of rat EGF receptor mRNAs, we analyzed the expression of EGF receptor mRNAs in the hepatoma cell line, AH66 and liver cells. Ten, 7 and 5 kb full-length and 2.7 kb truncated EGF receptor mRNAs were detected in both of them. The half-lives of the 10 and 2.7 kb EGF receptor mRNAs were determined in AH66 cells using a transcriptional inhibitor, 5,6-dichlororibofuranosylbenzimidazole. The half-lives of the 10 and 2.7 kb mRNAs were 1.2 and 11 h, respectively. These results indicated that the truncated mRNA is 4 times more stable than the full-length mRNA in rat cells. As for the stability, the role of a sequence of 3'-untranslated region of the EGF receptor mRNA was discussed.
Satoh
Y
Y
Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan.
Oomae
M
M
Hoshikawa
Y
Y
Izawa
M
M
Sairenji
T
T
Ichii
S
S
D000818:Animals; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology; D004355:Drug Stability; D066246:ErbB Receptors / Q000235:genetics*; D015870:Gene Expression; D006207:Half-Life; D006801:Humans; D008099:Liver / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
D019384:Nucleic Acid Synthesis Inhibitors; D012333:RNA, Messenger; D004004:Dichlororibofuranosylbenzimidazole; D066246:ErbB Receptors