Mitochondrial lipid peroxides and antioxidant enzymes in the liver following phorone-induced glutathione depletion.
The levels of lipid peroxidation product (malondialdehyde = MDA) and vitamin C and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were determined in the liver and its mitochondrial fraction, in the rats 2 and 18 hr after the injection of phorone, a glutathione (GSH) depleting agent. GSH levels decreased in liver homogenate and its mitochondrial fraction after 2 hr of phorone treatment, but these values returned to normal levels at 18 hr. In GSH depleted conditions, hepatic vitamin C levels increased, GSH-Px and SOD activities remained unchanged in mitochondrial and post-mitochondrial fractions. These results indicate that GSH depletion per se does not influence lipid peroxidation and GSH-Px and SOD activities in the liver and the mitochondrial fraction.
Mehmetçik
G
G
Art and Science Faculty, Near East University, Lefkosa, Turkey.
Alptekin
N
N
Toker
G
G
Uysal
M
M
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D005978:Glutathione / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007659:Ketones / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
D007659:Ketones; D008054:Lipid Peroxides; C018637:phorone; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid