title: Further studies on the recoupling effect of 6-ketocholestanol upon oxidative phosphorylation in uncoupled liver mitochondria.
abstract: The effect of 6-ketocholestanol was studied on CCCP-induced uncoupling in liver mitochondria, submitochondrial particles and cytochrome oxidase proteoliposomes. It was found that 6-ketocholestanol prevents and reverses uncoupling induced by nM concentrations of CCCP on the three systems assayed. As it was reported on kidney mitochondrial membranes [Chavez et al. (1996) FEBS Lett. 379, 305-308], the recoupling effect caused by 6-ketocholestanol on submitochondrial particles and proteoliposomes could be due to a diminution of membrane fluidity.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, México D.F., Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Cuéllar
authors.forename: J
authors.initials: J
authors.lastname: Ramirez
authors.forename: V M
authors.initials: VM
authors.lastname: Infante
authors.forename: E
authors.initials: E
authors.lastname: Chavez
mesh_terms: D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004161:Diphenylhexatriene / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000378:metabolism; D007653:Ketocholesterols / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D011510:Proteolipids / Q000378:metabolism; D051381:Rats; D013367:Submitochondrial Particles / Q000378:metabolism; D013696:Temperature; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D007653:Ketocholesterols; D011510:Proteolipids; D014475:Uncoupling Agents; C020485:proteoliposomes; D004161:Diphenylhexatriene; C063833:6-ketocholestanol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003576:Electron Transport Complex IV