Xenogeneic transfer of fetal liver and adult bone marrow-derived haemopoietic cells in rodents: changes in spleen colony differentials with increased doses of cells.
The effect of very high haemopoietic cell doses were investigated on the composition of splenic cell colonies/clusters in irradiated animals under xenogeneic circumstances. Differential cluster/colony counts from serial histological sections of the spleen were investigated before, and 9-12 days after transplantation of fetal liver- or adult bone marrow-derived haemopoietic cells following 5.0 to 8.5 Gy total body irradiation. Syngeneic as well as xenogeneic (mouse to rat and rat to mouse) transplantations were carried out. Cluster/colony differentials changed with the increase of the injected cell mass from 10(5) to 10(6) and 10(7) or more, i.e. the overwhelming erythroid pattern became trilinear even with xenogeneic transplants.
Gulya
E
E
2nd Department of Internal Medicine, Central Hospital of Ministry of the Interior, Budapest, Hungary.
Gábor Szabó
L
L
Kelemen
E
E
D000818:Animals; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D016332:Fetal Tissue Transplantation; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D016031:Liver Transplantation; D051379:Mice; D051381:Rats; D013154:Spleen / Q000166:cytology*; D014183:Transplantation, Heterologous