title: Analysis of phospholipid molecular species.
abstract: A method is described for analysing molecular species of glycerophospholipids. Diglycerides obtained by phospholipase C-catalysed hydrolysis of the phospholipid are separated into the diacyl- alkylacyl- and alkenylacyl- subclasses by HPLC on silicic acid. The molecular species of diacylglycerol are separated by HPLC of underivatised diglycerides on a reverse phase octadecyl-silica column.
authors.affiliation: Department of Biochemistry, University of Perugia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Vecchini
authors.forename: V
authors.initials: V
authors.lastname: Panagia
authors.forename: L
authors.initials: L
authors.lastname: Binaglia
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004075:Diglycerides / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D008027:Light; D008099:Liver / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D051381:Rats; D012542:Scattering, Radiation; D013025:Glycine max / Q000737:chemistry
chemical_list: D004075:Diglycerides; D006017:Glycolipids; D010743:Phospholipids; C015903:glycerolglycolipids