Physical mapping of potassium channel gene clusters on mouse chromosomes three and six.
Mammalian voltage-gated K channel genes have been divided into four subfamilies (Shaker, Shab, Shal, and Shaw) based on their sequence identity and similarity to related genes in Drosophila. Genetic mapping of the voltage-gated K channel genes has shown that similar multigene clusters exist on mouse Chr 3 and 6 and suggests that the clusters may have arisen through chromosomal duplication. In this report, YAC-based physical maps of the clustered mouse Shaker-like K channel genes have been constructed using restriction endonuclease and yeast chromosome fragmentation approaches. These data define the physical spacing as 5'-Kcna3-(60 kb)-Kcna2-(90 kb)-Kcna8-3' on Chr 3, and as 5'-Kcna6-(80 kb)-Kcna1-(110 kb)-Kcna5-3' on Chr 6, with all genes oriented in a head-to-tail manner within their respective clusters. These detailed physical maps of both K channel gene clusters provide additional support for the idea of an ancient genome tetraploidization event.
Street
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Department of Otolaryngology/Head and Neck Surgery, University of Washington School of Medicine, Seattle 98195, USA.
Tempel
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D000818:Animals; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D003001:Cloning, Molecular; D015342:DNA Probes; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D016521:Electrophoresis, Gel, Pulsed-Field; D019143:Evolution, Molecular; D008040:Genetic Linkage; D015640:Ion Channel Gating / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D051637:Shaker Superfamily of Potassium Channels; D014158:Transcription, Genetic
D015342:DNA Probes; D029721:Drosophila Proteins; D015221:Potassium Channels; C496929:Sh protein, Drosophila; D051637:Shaker Superfamily of Potassium Channels