title: Concentrations of lysosomal cysteine proteases are decreased in renal cell carcinoma compared with normal kidney.
abstract: Renal cell carcinoma contains significantly lower concentrations of the lysosomal cysteine proteases, cathepsins B, C, H, L and S, than does normal kidney, as shown by several methods, such as activity determination, enzyme-linked immunosorbent assay, immunoblotting and immunohistochemistry. The same low levels of enzyme activity and concentration have been determined in renal cell carcinoma metastases in the lung. Our results on the decreased concentration of cysteine peptidases at the protein level would seem to conflict with earlier results on an increased concentration of the cathepsin L mRNA in renal cell carcinoma.
authors.affiliation: Institute of Physiological Chemistry, Martin-Luther University, Halle (Saale), Germany.
authors.forename: H
authors.initials: H
authors.lastname: Kirschke
authors.forename: T
authors.initials: T
authors.lastname: Clausen
authors.forename: B
authors.initials: B
authors.lastname: Göhring
authors.forename: D
authors.initials: D
authors.lastname: Günther
authors.forename: E
authors.initials: E
authors.lastname: Heucke
authors.forename: F
authors.initials: F
authors.lastname: Laube
authors.forename: E
authors.initials: E
authors.lastname: Löwe
authors.forename: H
authors.initials: H
authors.lastname: Neef
authors.forename: H
authors.initials: H
authors.lastname: Papesch
authors.forename: S
authors.initials: S
authors.lastname: Peinze
authors.forename: G
authors.initials: G
authors.lastname: Plehn
authors.forename: U
authors.initials: U
authors.lastname: Rebmann
authors.forename: A
authors.initials: A
authors.lastname: Rinne
authors.forename: R
authors.initials: R
authors.lastname: Rüdrich
authors.forename: E
authors.initials: E
authors.lastname: Weber
mesh_terms: D002292:Carcinoma, Renal Cell / Q000201:enzymology*; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology*; D007680:Kidney Neoplasms / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D014407:Tumor Cells, Cultured
chemical_list: D004792:Enzyme Precursors; D002403:Cathepsins; C063905:procathepsin L; D056668:Cathepsin L