Genomic organization of the human DDX13 gene located between RD and RP1 in the class III MHC complex.
Recently we cloned a novel human cDNA homologous to yeast SKI2, reported a partial cDNA sequence, and mapped the gene to human chromosome 6p21 (Lee et al., 1995). It was a member of the DEAD/DExH family gene with seven conserved helicase domains; thus, it was named DDX13 consequently. We determined the complete genomic organization of the DDX13 gene. It consisted of 28 exons distributed over 11 kb of genomic DNA. An Alu element was present in introns 17 and 18, respectively. The major transcription start site was located 390 bp upstream from the translation initiation codon. The DDX13 gene was located in the class III region of the MHC between the genes coding for two other nuclear proteins, RD and RP1. The RD and DDX13 genes were oppositely oriented, and their first exons were overlapped. The distance between their first methionine codons was only 745 bp. It was of note that DDX13 and RD are in such proximity that their 5' regulatory regions overlap. The RP1 gene was located immediately downstream from the DDX13 gene in the same transcriptional orientation, and the distance between the stop codon of DDX13 and the translation initiation codon of RP1 was 2,272 bp.
Lee
S G
SG
Asan Institute for Life Sciences, Department of Biochemistry, University of Ulsan College of Medicine, Seoul, Korea.
Song
K
K
D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D018387:Codon, Initiator / Q000235:genetics; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D015894:Genome, Human; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data
D018387:Codon, Initiator; D017931:DNA Primers; D004247:DNA