Apoptosis of cord blood T lymphocytes by herpes simplex virus type 1.
We investigated apoptosis induced by herpes simplex virus type 1 (HSV-1) in cord blood T lymphocytes by using agarose gel electrophoresis, DNA content analysis and the terminal deoxytransferase (TdT)-mediated dUTP nick end-labelling (TUNEL) method. DNA fragmentation and the hypodiploid fraction in the cell cycle were both increased in HSV-1-infected CD4 and CD8 lymphocytes stimulated with phytohaemagglutinin (PHA) compared to mock-infected lymphocytes. The percentage of cells in the S phase was decreased in HSV-1-infected CD4 and CD8 lymphocytes. HSV-1 antigen, glycoprotein D (gD) and regulatory protein ICP27 were detected in 8-18% of the hypodiploid fraction of PHA-stimulated, HSV-1-infected lymphocytes. Apoptosis was induced not only in HSV-1 antigen-expressing cells but also in cells not expressing detectable viral proteins. Addition of anti-Fas antibody, anti-Fas-ligand antibody or a mixture of both had no effect on HSV-1-induced apoptosis, indicating that the Fas-Fas-ligand pathway did not contribute to HSV-1-induced apoptosis.
Ito
M
M
Department of Pediatrics, Mie University School of Medicine, Tsu, Japan. virus@clin.medic.mie-u.ac.jp
Koide
W
W
Watanabe
M
M
Kamiya
H
H
Sakurai
M
M
D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology; D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology / Q000821:virology; D002453:Cell Cycle; D053938:DNA Fragmentation; D005312:Fetal Blood / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000276:immunology* / Q000502:physiology; D006801:Humans; D007231:Infant, Newborn; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology / Q000821:virology*
D000956:Antigens, Viral; D010835:Phytohemagglutinins