The evaluation of the 29 and 31 kDa antigens in female Angiostrongylus cantonensis for serodiagnosis of human angiostrongyliasis.
The objective of this study was to evaluate the crude somatic extract of female Angiostrongylus cantonensis by ELISA and to evaluate the 29 kDa and the 31 kDa components of female worm by EITB for serodiagnosis of human angiostrongyliasis. Using ELISA, cross reaction occurred among all sera tested except capillariasis. The sensitivity, specificity, positive and negative predictive values of ELISA were found to be 100%, 66.8%, 27.1% and 100% respectively. Using EITB, The 29 kDa component cross-reacted with most of the heterologous sera while the 31 kDa component was recognized by 69.2% (18/26) sera from angiostrongyliasis patients, cross-reacted only with trichinellosis, trichuriasis and opisthorchiasis sera. This component may possibly used as reliable agents for angiostrongyliasis out of other tissue infected helminthiasis. The sensitivity, specificity, positive and negative predictive values of EITB for the 31.0 kDa component were 69.2%, 82.4%, 46.2% and 92.5% respectively.
Nuamtanong
S
S
Department of Parasitology, School of Medicine, Hiroshima University, Japan.
D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D016022:Case-Control Studies; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017206:Strongylida Infections / Q000276:immunology*
D000947:Antigens, Helminth