title: Potential involvement of both type I and type II mechanisms in M13 virus inactivation by methylene blue photosensitization.
abstract: We have investigated the mechanism of virus photoinactivation with methylene blue (MB) by conducting deuterium oxide (D2O), azide ion (N3-) and oxygen-dependent studies. Inactivation of M13 bacteriophage and singlet oxygen (1O2) generation by MB photosensitization were irradiation dose dependent. Inactivation of M13 was enhanced by D2O and inhibited by N3-, suggesting that 1O2 participates in M13 inactivation by MB photosensitization. However, N3- did not inhibit M13 inactivation completely. On the other hand, deoxygenating the reaction solution still caused 52-67% of M13 inactivation observed in the presence of oxygen. These results suggest that 1O2-mediated (Type II) and sensitizer-mediated (Type I) reactions may both play roles in M13 inactivation by MB photosensitization.
authors.affiliation: Hokkaido Red Cross Blood Center, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Ikebuchi
authors.forename: S J
authors.initials: SJ
authors.lastname: Wagner
authors.forename: M
authors.initials: M
authors.lastname: Kuwabara
authors.forename: N
authors.initials: N
authors.lastname: Kamo
authors.forename: S
authors.initials: S
authors.lastname: Sekiguchi
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D017104:Bacteriophage M13 / Q000187:drug effects*; D003903:Deuterium / Q000378:metabolism; D008751:Methylene Blue / Q000494:pharmacology*; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000494:pharmacology*; D026082:Singlet Oxygen
chemical_list: D000998:Antiviral Agents; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D003903:Deuterium; D009584:Nitrogen; D010100:Oxygen; D008751:Methylene Blue