title: Use of a single swab in multi-microbe or flex trans transport medium for detection of Chlamydia trachomatis by Roche Amplicor PCR and culture in specimens from two different patient populations.
abstract: The Roche Amplicor PCR increased the detection of Chlamydia trachomatis compared with culture in promptly processed clinical specimens from a local clinic (100 and 86.5%, respectively) and in samples with delayed processing transported from distant facilities (100 and 72.7%, respectively). A single swab collected in culture transport medium was used. Two media, Multi-Microbe and Flex Trans, were tested and found to be equally acceptable.
authors.affiliation: Virology Reference Laboratory, Department of Veterans Affairs Medical Center, West Haven, Connecticut 06516, USA.
authors.forename: C K
authors.initials: CK
authors.lastname: Fong
authors.forename: J
authors.initials: J
authors.lastname: Falcone
authors.forename: M L
authors.initials: ML
authors.lastname: Landry
mesh_terms: D000554:Ambulatory Care Facilities; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000254:growth & development* / Q000302:isolation & purification*; D003470:Culture Media / Q000378:metabolism; D005260:Female; D006787:Hospitals, Veterans; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D013048:Specimen Handling; D014522:Urethral Diseases / Q000382:microbiology; D002577:Uterine Cervical Diseases / Q000382:microbiology
chemical_list: D003470:Culture Media; D011933:Reagent Kits, Diagnostic