Polymorphism of extracellular superoxide dismutase (EC-SOD) gene: relation to the mutation responsible for high EC-SOD level in serum.
Extracellular superoxide dismutase (EC-SOD) with amino acid substitution R213G generated by the nucleotide substitution 760C-->G in the heparin binding domain is responsible for the high EC-SOD level in serum. We identified the two DNA polymorphic sites in the coding region of EC-SOD gene related to the 760C-->G and determined the allele frequencies. The polymorphism were A and G at nucleotide position (nt.) 241 and C and T at nt. 280 near the N-terminal. The haplotype frequencies in Japanese were 241A280C: 0.45, 241G280T: 0.37, and 241G280C: 0.18. The haplotype of 241A280T did not exist. The mutation 760C-->G must occur on the allele having the haplotype of 241G280T.
Yamada
H
H
First Department of Internal Medicine, Aichi Medical University, Japan.
Yamada
Y
Y
Adachi
T
T
Goto
H
H
Ogasawara
N
N
Futenma
A
A
Kitano
M
M
Miyai
H
H
Fukatsu
A
A
Hirano
K
K
Kakumu
S
S
D005110:Extracellular Space / Q000201:enzymology*; D006239:Haplotypes; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000097:blood* / Q000235:genetics*
D013482:Superoxide Dismutase