Seasonal changes in gonadal activity and the effects of stress on reproductive hormones in the common snapping turtle, Chelydra serpentina.
The seasonal gonadal cycle (including gonadal histology, sex steroids, and gonadotropins) was studied in freshly captured common snapping turtles, Chelydra serpentina, from Wisconsin, and the effects of capture stress were evaluated. The ovarian and testicular cycles are shorter than those reported in other freshwater turtles; the cycles commence in mid-May and terminate in early September, immediately after the completion of gonadal growth and maturation. In the female, testosterone (T), 17beta-estradiol (E2), and progesterone (Pro) were highly correlated with follicular growth and vitellogenesis. Ovulation in captivity and under natural conditions occurred after mid-May. In captivity, ovulation was a rapid process (24-48 hr); as the follicles descended into the uterine horns there was a significant increase in E2 and Pro and eggs were retained in the uterine horns for about 2 weeks before oviposition. In the male, T was significantly correlated with testicular growth and spermiation. Follicle-stimulating hormone (FSH) reached significantly higher levels (P < 0.01) in males (8.99 +/- 0.38 ng/ml) than in females (2.66 +/- 0.22 ng/ml), but luteinizing hormone was undetectable in both sexes. FSH was not correlated with the steroids in either sex. Sex steroids and FSH began to rise before spermiation and vitellogenesis and remained elevated until completion of gonadal growth and maturation. Leydig cells, the main source of plasma androgen in this species, became active shortly after emergence from hibernation and remained steroidogenically active for the rest of the cycle. Sertoli cells became active only after spermatogenesis was under way but also stayed active for the rest of the summer. Courtship and mating behaviors were observed in spring, summer, and fall. The snapping turtle is strictly aquatic with no basking behavior and limited behavioral thermoregulation so there is little daily fluctuation in body temperature. Environmental correlates indicate that the snapping turtle is temperature dependent: recrudesence occurs with a slight increase in water temperature during spring and early summer, while a dramatic drop in gonadal activity accompanies a slight decrease in temperature in fall. Changes in temperature may underlie changes in gonadal activity in the face of relatively stable FSH. Male turtles subjected to captivity and periodic blood sampling show a significant decline in T. The hormonal levels continued to decline whether the turtles are exposed to optimum or extreme temperatures. However, there is more rapid decline in T values in animals with regressed testes (June) than in those with well-developed testes (July). Male and female turtles kept in captivity at different phases of the cycle exhibit different patterns and degrees of response to stress, possibly related to the hormonal levels and the condition of the gonads.
Mahmoud
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Department of Biology and Microbiology, University of Wisconsin, Oshkosh, Wisconsin, 54901, USA.
Licht
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D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D008297:Male; D010053:Ovary / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D011863:Radioimmunoassay; D012098:Reproduction / Q000502:physiology; D012621:Seasons; D012727:Sex Characteristics; D013315:Stress, Psychological / Q000378:metabolism / Q000503:physiopathology*; D013737:Testis / Q000166:cytology / Q000502:physiology*; D013739:Testosterone / Q000097:blood; D014426:Turtles / Q000378:metabolism*
D012739:Gonadal Steroid Hormones; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol