title: Chemoattractant factors (IP-10, MIP-1alpha, IL-16) mRNA expression in mononuclear cells from different tissues during acute SIVmac251 infection of macaques.
abstract: We have used semiquantitative RT-PCR to monitor the expression of mRNA encoding chemoattractant factors IP-10, MIP-1alpha, and IL-16 in freshly isolated peripheral blood mononuclear cells (PBMCs), lymph node mononuclear cells (LNMCs), and mononuclear cells obtained after bronchoalveolar lavages (BALMCs) of two cynomolgus macaques inoculated intravenously with a pathogenic isolate of simian immunodeficiency virus, SIVmac251. Concomitant with the peak of systemic viral replication (two weeks after experimental inoculation) and proinflammatory cytokine IL-6 mRNA expression, high levels of MIP-1alpha and IP-10 mRNA were produced in LNMCs and BALMCs. In BALMCs, in which we have reported a marked progressive overexpression of IFN-gamma mRNA coinciding with an increase in the CD8+ lymphocyte percentages, we noticed a progressive overexpression of IL-16 mRNA. Our results suggest the role of chemokines IP-10, MIP-1alpha, and IL-16 in the development of inflammatory and immune responses during the early stages of lentiviral infection.
authors.affiliation: Centre de Recherches du Service de Santé des Armées Emile Pardé, Commissariat à l'Energie Atomique, DSV/DRM, Institut Paris-Sud sur lesCytokines, Fontenay aux Roses, France.
authors.forename: A
authors.initials: A
authors.lastname: Chéret
authors.forename: R
authors.initials: R
authors.lastname: Le Grand
authors.forename: P
authors.initials: P
authors.lastname: Caufour
authors.forename: O
authors.initials: O
authors.lastname: Neildez
authors.forename: F
authors.initials: F
authors.lastname: Matheux
authors.forename: F
authors.initials: F
authors.lastname: Théodoro
authors.forename: F
authors.initials: F
authors.lastname: Boussin
authors.forename: B
authors.initials: B
authors.lastname: Vaslin
authors.forename: D
authors.initials: D
authors.lastname: Dormont
mesh_terms: D000818:Animals; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054357:Chemokine CXCL10; D018925:Chemokines / Q000096:biosynthesis*; D019743:Chemokines, CXC; D007111:Immunity, Cellular; D007249:Inflammation / Q000276:immunology; D019410:Interleukin-16 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000821:virology; D008198:Lymph Nodes / Q000166:cytology; D008252:Macaca fascicularis; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
chemical_list: D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054357:Chemokine CXCL10; D018925:Chemokines; D019743:Chemokines, CXC; D019410:Interleukin-16; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger