Annexin I targets S100C to early endosomes.
Immunofluorescence and subcellular fractionation localize annexin I and the EF hand protein S100C to the same membranous structures which in part correspond to transferrin receptor-positive endosomes. The association of S100C with endosomal membranes is strictly dependent on annexin I binding since a D91stop-S100C mutant protein, in which the residues essential for annexin I binding have been removed, fails to colocalize with membraneous structures. This indicates that annexin I and S100C form a complex in vivo and that the endosomal localization of this complex is mediated through an interaction of annexin I with the endosomal membrane.
Seemann
J
J
Department of Biochemistry, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Weber
K
K
Gerke
V
V
D000818:Animals; D017305:Annexin A1 / Q000378:metabolism / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D006224:Cricetinae; D011992:Endosomes / Q000737:chemistry / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D009418:S100 Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D013347:Subcellular Fractions / Q000737:chemistry; D014162:Transfection
D017305:Annexin A1; D002135:Calcium-Binding Proteins; D009418:S100 Proteins; C072337:S100A11 protein, human