Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.
A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.
Kinoshita
Y
Y
Department of Functional Materials Science, Saitama University, 255 Shimo-okubo, Urawa 338, Japan.
Nishigaki
K
K
Husimi
Y
Y
D001710:Biotin; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D007553:Isotope Labeling; D015336:Molecular Probe Techniques; D012313:RNA / Q000737:chemistry*; D011130:RNA Ligase (ATP)
D005456:Fluorescent Dyes; D012313:RNA; D001710:Biotin; D004247:DNA; D011130:RNA Ligase (ATP)