title: Modulation of human T lymphocyte proliferation by 4-hydroxynonenal, the bioactive product of neutrophil-dependent lipid peroxidation.
abstract: The proliferative capacity of immune cells is known to be sensitive to conditions of oxidative stress and lipid peroxidation. We tested the hypothesis that activated neutrophils can induce peroxidation in extracellular lipid substrates, and evaluated the effects of 4-hydroxy-2,3-trans-nonenal (4-HNE)--the most reactive aldehydic product of lipid peroxidation--on mitogen-induced proliferation of human T lymphocytes. Neutrophils activated in the presence of extracellular lipid substrates (liposomes, cellular membranes) induced lipid peroxidation. By means of cytoimmunofluorescent labeling and confocal microscopy, the binding of 4-HNE to surface and cytoplasmic proteins of activated neutrophils was observed. Short (20 min) pre-treatment of cells with low concentrations of 4-HNE were able to dose-dependently decrease the proliferation of human peripheral blood lymphocytes challenged with PHA or anti-CD3 monoclonal antibody OKT3, as well as the proliferation of a tetanus specific human T-cell line challenged with tetanus toxoid. In these conditions, the binding of 4-HNE to surface and cytoplasmic proteins of lymphocytes was also observed. When the proliferative capacity of peripheral blood lymphocytes was monitored over several days after 4-HNE treatment and PHA challenge, a recovery and a rebound in cell proliferation was observed. Data reported indicate that the lipid peroxidation promoted by activated neutrophils can exert modulatory effects on the responsivity of human T cells, through the action of its most reactive product, 4-HNE.
authors.affiliation: Istituto di Patologia Generale dell'Università, Siena, Italia.
authors.forename: C
authors.initials: C
authors.lastname: Cambiaggi
authors.forename: S
authors.initials: S
authors.lastname: Dominici
authors.forename: M
authors.initials: M
authors.lastname: Comporti
authors.forename: A
authors.initials: A
authors.lastname: Pompella
mesh_terms: D000447:Aldehydes / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D006801:Humans; D007502:Iron Chelating Agents; D015227:Lipid Peroxidation; D018375:Neutrophil Activation; D009504:Neutrophils / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
chemical_list: D000447:Aldehydes; D007502:Iron Chelating Agents; C027576:4-hydroxy-2-nonenal