Mucins secreted by a transformed cell line derived from human tracheal gland cells.
High-molecular-mass glycoconjugates are secreted by the continuous cell line MM-39, which has been obtained from cultured human tracheal gland cells transformed by simian virus 40. They were purified on Sepharose(R) CL-4B and then by two steps of density-gradient centrifugation. High-molecular-mass glycoproteins resistant to digestion by hyaluronidase, chondroitin ABC lyase and heparitinase were obtained, in addition to hyaluronic acid and proteoglycans. They were susceptible to beta-elimination. They contained polylactosaminoglycan chains as well as carbohydrate chains with a terminal sialic acid in the NeuAc alpha2-3 sequence. Most of them have a buoyant density of 1.45 g/ml in CsCl-density-gradient centrifugation, except for MUC1. The MM-39 cells were also characterized by a high expression of MUC1 and MUC4 genes, but they did not express MUC2, MUC3, MUC5B and MUC5AC. Therefore the MM-39 cells synthesized mucin-like glycoproteins as well as lysozyme and mucous proteinase inhibitor [Merten, Kammouni, Renaud, Birg, Mattéi and Figarella (1996) Am. J. Respir. Cell. Mol. Biol. 15, 520-528]; they should be considered as having a mixed, both serous and mucous, phenotype.
Lo-Guidice
J M
JM
Unité INSERM 377, Lille, France.
Merten
M D
MD
Lamblin
G
G
Porchet
N
N
Houvenaghel
M C
MC
Figarella
C
C
Roussel
P
P
Perini
J M
JM
D002461:Cell Line, Transformed; D002499:Centrifugation, Density Gradient; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005088:Exocrine Glands / Q000166:cytology* / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008970:Molecular Weight; D009077:Mucins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D014132:Trachea / Q000166:cytology* / Q000378:metabolism*
D006023:Glycoproteins; D009077:Mucins; D012333:RNA, Messenger