Genomic organization and functional characterization of the mouse GalR1 galanin receptor.
Galanin mediates diverse physiological functions in digestive, endocrine, and central nervous systems through G-protein-coupled receptors. Two galanin receptors have been cloned but the gene structures are unknown. We report genomic and cDNA cloning of the mouse GalR1 galanin receptor and demonstrate that the coding sequence is uniquely divided into three exons encoding the N-terminal portion through the fifth transmebrane domain, the third intracellular loop, and the sixth transmembrane domain through the C-terminus. Functional analysis of the encoded cDNA revealed active ligand binding and intracellular signaling. The expression is detected in brain, spinal cord, heart and skeletal muscle.
Wang
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S
Department of CNS/CV Biological Research, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA. suke.wang@spcorp.com
He
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Maguire
M T
MT
Clemmons
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Burrier
R E
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Guzzi
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Strader
C D
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Parker
E M
EM
Bayne
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ML
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002490:Central Nervous System / Q000737:chemistry; D003001:Cloning, Molecular; D005576:Colforsin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D005091:Exons / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone; D005576:Colforsin; D000242:Cyclic AMP