Evaluation of capillary electrophoresis with post-column derivatization and laser-induced fluorescence detection for the determination of substance P and its metabolites.
A method for the detection of substance P and its metabolites using capillary electrophoresis with post-capillary derivatization and laser-induced fluorescence detection is described. The post-capillary chemical derivatization system employs naphthalene-2,3-dicarboxaldehyde and beta-mercaptoethanol. Two reactor designs were evaluated for the determination of substance P and its metabolites. The fluorescent spectroscopic properties of the derivatives under optimal separation conditions were also examined. The final system was evaluated for the investigation of substance P metabolism in brain following perfusion of the striatum with substance P using microdialysis sampling.
Kostel
K L
KL
Department of Pharmaceutical Chemistry and Center for Bioanalytical Research, University of Kansas, Lawrence 66047, USA.
Lunte
S M
SM
D000818:Animals; D001923:Brain Chemistry; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods; D007834:Lasers; D008297:Male; D008623:Mercaptoethanol; D017551:Microdialysis; D009281:Naphthalenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence; D013373:Substance P / Q000032:analysis* / Q000378:metabolism
D002021:Buffers; D009281:Naphthalenes; D013373:Substance P; D008623:Mercaptoethanol; C027717:2,3-naphthalenedicarboxaldehyde