GABAergic neurons with AMPA GluR1 and GluR2/3 immunoreactivity in the rat striate cortex.
The co-localization of GABA with AMPA receptor subunits GluR1 or GluR2/3 was analysed in the striate cortex of adult rats by post-embedding immunocytochemistry in semithin sections. Adjacent 1 micron semithin sections of four brains were alternately incubated with specific antibodies against GABA and the GluR1 and GluR2/3 subunits. The post-embedding immunocytochemistry showed that 38% of GABAergic neurons contained the GluR1 subunit and 10% contained the GluR2/3 subunits. Previous work has shown GluR1 immunoreactivity in non-pyramidal neurons and GluR2/3 immunoreactivity in pyramidal neurons. However, this study is the first to demonstrate that there are GABAergic neurons co-localized with GluR2/3 AMPA subunits. Additionally, this study provides quantitative estimations of the laminar distribution of GABAergic and non-GABAergic cells containing the AMPA GluR1 and GluR2/3 subunits.
Gutiérrez-Ibarluzea
I
I
Department of Neuroscience, School of Medicine and Dentistry, University of the Basque Country, Leioa, Biscay, Spain.
Mendizabal-Zubiaga
J L
JL
Reblet
C
C
Bueno-López
J L
JL
D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000032:analysis*; D014793:Visual Cortex / Q000166:cytology*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D018091:Receptors, AMPA; D005680:gamma-Aminobutyric Acid; C104723:glutamate receptor ionotropic, AMPA 3; C104722:glutamate receptor ionotropic, AMPA 2