title: Increased alkaline phosphatase isoforms in autoimmune diseases.
abstract: We found significant increases in ALP and ALP isoform band 10 in the serum of patients with early insulin-dependent diabetes, rheumatoid arthritis, and in those with multiple sclerosis during periods of disease exacerbation as compared with healthy controls. The ALP isoforms were assayed by isoelectric focusing. Our data suggest that the increase in ALP and ALP-10 closely reflects the abnormal activation of T lymphocytes that is common in autoimmune diseases, and that the source of the ALP-10 is activated T lymphocytes. ALP-10 is a sensitive but nonspecific marker of an active autoimmune process and appears to have the ability to detect abnormal T-cell activation. ALP-10 may be a useful test in the screening for autoimmune disorders.
authors.affiliation: Department of Pathology, Ohio State University, Columbus 43210, USA.
authors.forename: A N
authors.initials: AN
authors.lastname: Hanna
authors.forename: W J
authors.initials: WJ
authors.lastname: Waldman
authors.forename: J A
authors.initials: JA
authors.lastname: Lott
authors.forename: S C
authors.initials: SC
authors.lastname: Koesters
authors.forename: A M
authors.initials: AM
authors.lastname: Hughes
authors.forename: D J
authors.initials: DJ
authors.lastname: Thornton
mesh_terms: D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood* / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000201:enzymology / Q000276:immunology; D001327:Autoimmune Diseases / Q000201:enzymology* / Q000276:immunology; D015415:Biomarkers; D002648:Child; D016207:Cytokines / Q000494:pharmacology; D003587:Cytomegalovirus / Q000502:physiology; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology / Q000276:immunology; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood* / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000201:enzymology / Q000276:immunology; D008213:Lymphocyte Activation; D009103:Multiple Sclerosis / Q000201:enzymology / Q000276:immunology; D012217:Rheumatoid Factor / Q000032:analysis / Q000097:blood; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
chemical_list: D015415:Biomarkers; D016207:Cytokines; D007527:Isoenzymes; D012217:Rheumatoid Factor; D000469:Alkaline Phosphatase