title: Influence of a proton pump inhibitor-based therapy on Helicobacter pylori strain selection.
abstract: The influence of the proton pump inhibitor lansoprazole on strain diversity in Helicobacter pylori infected patients was investigated. Multiple isolates of Helicobacter pylori obtained pre- and post-therapy from gastric antral and body biopsies in 22 patients were compared using the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) for analysis. Post-therapy strains exhibiting novel RAPD-profiles were found in 5 of 22 patients (4 of 11 patients treated with lansoprazole alone and 1 of 11 patients treated with lansoprazole plus amoxicillin). Proton pump inhibition may affect the microecology of the stomach by influencing the colonisation patterns of specific strains.
authors.affiliation: School of Microbiology and Immunology, University of New South Wales, Sydney, Australia.
authors.forename: S L
authors.initials: SL
authors.lastname: Hazell
authors.forename: H M
authors.initials: HM
authors.lastname: Mitchell
authors.forename: G
authors.initials: G
authors.lastname: Hanna
authors.forename: G
authors.initials: G
authors.lastname: Daskalopoulos
mesh_terms: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001706:Biopsy; D004311:Double-Blind Method; D005753:Gastric Mucosa / Q000382:microbiology; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D064747:Lansoprazole; D009853:Omeprazole / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D054328:Proton Pump Inhibitors; D011706:Pyloric Antrum / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000897:Anti-Ulcer Agents; D054328:Proton Pump Inhibitors; D064747:Lansoprazole; D009853:Omeprazole