3D structural information as a guide to protein engineering using genetic selection.
A great variety of protein systems have been investigated in the past year using structure-guided evolutionary strategies. On the basis of available 3D structural information, critical regions of proteins have been targeted for randomizing mutagenesis and active variants of the corresponding genes have been selected. These approaches help characterize structural and mechanistic features of proteins and have important implications for design.
Kast
P
P
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA. pjokast@scripps.edu
Hilvert
D
D
D001665:Binding Sites; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D013329:Structure-Activity Relationship
D019151:Peptide Library; D011506:Proteins