[The effect of splenectomy on alveolar macrophages morphology and function].
OBJECTIVE
To study the morphological and functional changes of pulmonary alveolar macrophages of rats after splenectomy, and the applied effects of splenic tissue autotransplantation in practice.


METHODS
87 Wistar rats were randomly divided into shamoperation group, splenectomy group and splenic tissue autotransplantation group. Six months after splenectomy, alveolar macrophges were subjected to brochoalveolar lavage described by Shennib. The dynamic survival and adherent rate of alveolar macrophages in culture, lysosomal enzyme content, hydrogen peroxide production and expression level of interleukin 1 (IL-1) activity of alveolar macrophages were quantitatively measured. The alveolar macrophages ultrastructure was observed by utilizing transmission electron microscope.


RESULTS
The splenectomized rat's alveolar macrophages were different from alveolar macrophages of sham-operated rats. Their surface filopodia was reduced and shortened, lysosome fewer and its acid phosphatase quantity decreased, adherence postponed, hydrogen peroxide production and expression of IL-1 activity impaired. Splenic tissue autotransplantation fairly restored the splenic effects on maturation and function of alveolar macrophages.


CONCLUSIONS
Splenic tissue autotransplantation is a simple useful operation for preserving splenic function after splenectomy.
Leng
J
J
Department of Applied Anatomy and Operative Surgery, Third Military Medical University, Chongqing.
Chen
W
W
Zhang
J
J
D000818:Animals; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000166:cytology* / Q000378:metabolism; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000637:transplantation; D013156:Splenectomy / Q000009:adverse effects*; D014182:Transplantation, Autologous
D007375:Interleukin-1; D006861:Hydrogen Peroxide