title: Identification of a Klebsiella pneumoniae strain associated with nosocomial urinary tract infection.
abstract: To differentiate between relapse of infection and reinfection of the urinary tract due to Klebsiella pneumoniae, 33 K. pneumoniae isolates collected from 20 patients with spinal cord injury (SCI) over 2 years were typed by genomic fingerprinting by repetitive-element PCR. Clinical isolates obtained from the same patients with recurrent episodes of urinary tract infection (UTI) revealed identical genomic fingerprints indicating relapse of UTI due to K. pneumoniae, despite appropriate antibiotic therapy. Seventeen isolates obtained from 8 of the 20 SCI patients shared a common genotype, termed RD6. Among non-SCI patients residing in other nursing units, the RD6 genotype was found in 5 of 10 patients with K. pneumoniae UTI but in only 1 of 20 patients with K. pneumoniae infection that did not involve the urinary tract, suggesting a strong association of this genotype with UTI. All RD6 isolates exhibited strong adherence (> or =50 adherent bacteria per cell) to HEp-2 cells, whereas other K. pneumoniae isolates generally did not adhere to or adhered very weakly to HEp-2 cells (< or =5 adherent bacteria per cell). Adherence was inhibited either by 4% D-mannose or by anti-type 1 fimbrial rabbit serum. These results suggest that the capacity of K. pneumoniae RD6 isolates to cause UTI may be mediated by its striking adherence to mammalian cells.
authors.affiliation: Department of Physical Medicine and Rehabilitation, Baylor College of Medicine and the Veterans Affairs Medical Center, Houston, Texas 77030, USA.
authors.forename: K S
authors.initials: KS
authors.lastname: Kil
authors.forename: R O
authors.initials: RO
authors.lastname: Darouiche
authors.forename: R A
authors.initials: RA
authors.lastname: Hull
authors.forename: M D
authors.initials: MD
authors.lastname: Mansouri
authors.forename: D M
authors.initials: DM
authors.lastname: Musher
mesh_terms: D019138:Antibodies, Blocking / Q000276:immunology; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D003428:Cross Infection / Q000453:epidemiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D010861:Fimbriae, Bacterial / Q000276:immunology; D006787:Hospitals, Veterans; D006801:Humans; D007710:Klebsiella Infections / Q000175:diagnosis* / Q000453:epidemiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000302:isolation & purification*; D008358:Mannose / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012008:Recurrence; D013119:Spinal Cord Injuries / Q000150:complications; D014552:Urinary Tract Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D019138:Antibodies, Blocking; D004269:DNA, Bacterial; D008358:Mannose