title: Protein splicing in the yeast Vma1 protozyme: evidence for an intramolecular reaction.
abstract: Protein splicing is an autocatalytic reaction of a single polypeptide in which a spliced intervening sequence is excised out and the two external regions are ligated with the peptide bond to yield two mature proteins. We examined the reaction mechanism using a folding-dependent in vitro protein splicing system. Protein splicing proceeds at an optimal pH of 7 and is an intramolecular reaction. The reaction is not inhibited by potential protease inhibitors, suggesting that its mechanism is different from those catalyzed by known proteases.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Satow
authors.forename: Y
authors.initials: Y
authors.lastname: Ohya
authors.forename: Y
authors.initials: Y
authors.lastname: Anraku
mesh_terms: D002384:Catalysis; D004706:Endodeoxyribonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011480:Protease Inhibitors / Q000378:metabolism / Q000494:pharmacology; D017510:Protein Folding; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019154:Protein Splicing / Q000187:drug effects; D006180:Proton-Translocating ATPases; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity
chemical_list: D011480:Protease Inhibitors; D011498:Protein Precursors; D029701:Saccharomyces cerevisiae Proteins; D004706:Endodeoxyribonucleases; D006180:Proton-Translocating ATPases; C081687:VMA1 protein, S cerevisiae