Bordetella pertussis-specific Th1/Th2 cells generated following respiratory infection or immunization with an acellular vaccine: comparison of the T cell cytokine profiles in infants and mice.
In an investigation of cell-mediated immunity against Bordetella pertussis, we found that B. pertussis infection in infants and in mice was associated with the induction of antigen-specific T cells that secrete IFN-g and IL-2, but not IL-4 or IL-5. This cytokine profile is characteristic of Th1 cells that mediate cellular immune responses against a range of intracellular pathogens. An examination of cytokine production following immunization with a three-component acellular vaccine, comprising inactive PT, FHA and pertactin adsorbed to alum, demonstrated that spleen cells from vaccinated mice produced high levels of IL-5, but no detectable IFN-g and low levels of IL-2. In contrast, peripheral blood mononuclear cells from vaccinated infants produced IL-2, IL-5 and IFN-g. These findings highlight significant differences in the immune responses generated by vaccination and natural infection with B. pertussis and demonstrate that the T-cell response induced with an acellular vaccine, although dominated by type 2 cytokines in mice, is more heterogeneous in infants with a Th0 or mixed Th1/Th2 cytokine profile.
Ryan
M
M
Infection and Immunity Laboratory, Maynooth College, Co Kildare, Ireland.
Gothefors
L
L
Storsaeter
J
J
Mills
K H
KH
D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001886:Bordetella pertussis / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology*; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D006801:Humans; D007111:Immunity, Cellular; D007223:Infant; D051379:Mice; D018417:Th1 Cells / Q000276:immunology* / Q000382:microbiology; D018418:Th2 Cells / Q000276:immunology* / Q000382:microbiology; D014611:Vaccination; D014917:Whooping Cough / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control
D000907:Antibodies, Bacterial; D016207:Cytokines; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines