Expression of small RNAs by Bacillus sp. strain PS3 and B. subtilis cells during sporulation.
A small RNA sequence identified in an rRNA-tRNA cluster from the thermophilic Bacillus sp. strain PS3 was examined. An oligonucleotide probe specific for the RNA bound to multiple restriction fragments in Bacillus sp. strain PS3 DNA, thus several copies of this sequence occur in its genome. Similar findings were observed using DNA from B. subtilis, B. stearothermophilus, Escherichia coli, Staphylococcus aureus, Haemophilus influenzae and Thermus thermophilus. This sequence apparently is widespread in the eubacteria. Northern analysis of RNA from sporulating Bacillus sp. strain PS3 and B. subtilis cells revealed RNA species homologous to the probe in both bacteria. Expression of the small RNA in B. subtilis depended on sigma H.
Fink
P S
PS
Department of Microbiology and Immunology, Wright State University, Dayton, OH 45435-0002, USA. pfink@desire.wright.edu
Soto
A
A
Jenkins
G S
GS
Rupert
K S
KS
D001407:Bacillus / Q000235:genetics* / Q000502:physiology; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D012329:RNA, Bacterial / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012348:RNA, Transfer, Asn / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology; D013171:Spores, Bacterial / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D012348:RNA, Transfer, Asn; D012808:Sigma Factor; C087737:Spo0H protein, Bacillus subtilis; D014157:Transcription Factors