Analysis of Chinese isolates of Trichinella spiralis by molecular biotechnology.
OBJECTIVE
To investigate the relationships among Trichinella spiralis isolates obtained from different hosts and geographical regions in China.


METHODS
Six isolates of Trichinella spiralis obtained from Changchun, Tianjin, Xi'an, Henan and Yunnan were detected. All the isolates were collected from pigs or dogs. Restriction fragment length polymorphism (RFLP), isoenzyme electrophoresis and soluble protein analysis were used.


RESULTS
Distinctive banding patterns in restriction maps were seen between Changchun and other isolates studied. A cloned specific repetitive DNA sequence (1.12 kb) from Changchun isolate was then selected to prepare probe for Southern blotting of DNA fragments. The hybridizing banding patterns among the isolates were different and only the Changchun isolate showed the 1.12 kb band. The Changchun isolate was also distinguishable from the other isolates in isoenzyme patterns of GP1, G6PD, HK, 6PGDH and AK, as well as in isoelectric focusing electrophoresis where the Changchun isolate presented a specific band at 4.1 P1.


CONCLUSIONS
There are at least two different biological types of Trichinella spiralis in China and the differences between them may be attributed to different geographical origins and/or different hosts.
Zhang
Y
Y
Beijing Tropical Medicine Research Institute.
Wang
H
H
Lao
W
W
Zhou
M
M
Wu
Z
Z
Liu
H
H
Shi
H
H
D000818:Animals; D002681:China; D004285:Dogs / Q000469:parasitology*; D014644:Genetic Variation; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D013552:Swine / Q000469:parasitology*; D017160:Trichinella spiralis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification