Molecular approaches to identify novel genes expressed in Arabidopsis thaliana.
Our laboratory is engaged in an effort to identify genes expressed primarily during plant embryogenesis. Genes which exhibit unique expression profiles in the plant are also being sought. To this end, several methods to identify and clone novel genes based on specific expression patterns have been developed. These methods include virtual subtraction, differential display and other PCR based technologies. In addition to this, a yeast one-hybrid approach has been established to identify transcription factors which regulate these genes. To date, this work has identified several novel genes.
Nuccio
M L
ML
Department of Biology, Texas A&M University, College Station 77843-3258, USA.
Li
Z
Z
Hsieh
T F
TF
Kim
S Y
SY
Thomas
T
T
D017360:Arabidopsis / Q000196:embryology / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis / Q000737:chemistry; D015870:Gene Expression; D017343:Genes, Plant; D005818:Genetic Engineering; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D018749:RNA, Plant