title: The use of two different APC resistance assay systems provides optimal sensitivity and specificity for diagnosing genetic APC resistance.
abstract: We compared the performance characteristics of a commercial dilute Russell's viper venom (DRVV)-based APC resistance assay (Gradipore PC Impedance Test) to a routinely utilized commercial APTT based assay (Coatest APC Resistance Assay). The DRVV based assay offers improved sensitivity and specificity for the factor V Leiden mutation. However, the routine use of both assays provides optimum reliability for diagnosis of genetic APC resistance. Our results suggest that when both tests are either positive or negative, DNA analysis is unnecessary. Interference by lupus anticoagulants is dramatically minimized by the phospholipid rich DRVV reagent used in the assay and it is insensitive to high factor VIII activity. Additionally, discrepant functional assay results allow identification of patients who may have an acquired APC resistant phenotype.
authors.affiliation: Haematology Department, Princess Alexandra Hospital, Wooloongabba, Queensland, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Solano
authors.forename: A L
authors.initials: AL
authors.lastname: Hogg
authors.forename: R J
authors.initials: RJ
authors.lastname: Saal
authors.forename: D C
authors.initials: DC
authors.lastname: Scott
authors.forename: R G
authors.initials: RG
authors.lastname: Cobcroft
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004351:Drug Resistance; D005165:Factor V / Q000032:analysis* / Q000235:genetics; D006801:Humans; D009154:Mutation; D011486:Protein C / Q000494:pharmacology*; D012680:Sensitivity and Specificity
chemical_list: D011486:Protein C; C095381:factor V Leiden; D005165:Factor V