Analysis of zolpidem in human plasma by high-performance liquid chromatography with fluorescence detection: application to single-dose pharmacokinetic studies.
Zolpidem, an imidazopyradine hypnotic agent, can be quantitated by high-performance liquid chromatography (HPLC) with fluorescence detection. After the addition of a structurally related internal standard (propyl-zolpidem), plasma samples were double-extracted at neutral pH with toluene-isoamyl alcohol or benzene-isoamyl alcohol. The organic extracts were evaporated to dryness, reconstituted with mobile phase, and analyzed by HPLC using a C-18 reversed-phase column, a mobile phase of acetonitrile-50mM potassium dihydrogen phosphate (50:50), and fluorescence detection with excitation and emission wavelengths of 254 and 390 nm, respectively. The lower limit of reliable quantitation was in the range of 1-2.5 ng/mL. The method is applicable to single-dose pharmacokinetic studies of zolpidem in humans.
Durol
A L
AL
Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Boston, MA 02111, USA.
Greenblatt
D J
DJ
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004334:Drug Administration Schedule; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood* / Q000493:pharmacokinetics*; D008297:Male; D011725:Pyridines / Q000097:blood* / Q000493:pharmacokinetics*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D000077334:Zolpidem
D006993:Hypnotics and Sedatives; D011725:Pyridines; D000077334:Zolpidem