title: Mucosal inflammation in pediatric diversion colitis: a quantitative analysis.
abstract: BACKGROUND
  Diversion colitis commonly occurs in bypassed segments of colorectum, and has been described qualitatively in Hirschsprung's disease patients with colostomies. The objective of this study was to characterize quantitatively the changes in the inflammatory cell population in the mucosa of children with diversion colitis.
  
  
  METHODS
  Paraffin blocks of well-oriented, full-thickness colorectal tissues were obtained from 15 children with diversion colitis (all with Hirschsprung's disease), four pediatric controls and four adult controls. Sections were immunostained for B and T lymphocytes, macrophages, IgG, IgM, and IgA. Measurements were made referent to a standard length of muscularis mucosae. Lymphoid follicles were counted and the areas occupied by B and T cells were determined by image analysis. Cells in the interfollicular lamina propria were counted separately, but IgA-containing plasma cells were too abundant to enumerate.
  
  
  RESULTS
  Pediatric diversion colitis was characterized by enlarged and more numerous lymphoid follicles with approximately four times as many B lymphocytes and twice as many T lymphocytes in the follicular compartment of the mucosa when compared to pediatric controls. The interfollicular mucosa was thickened (499 +/- 27 versus 380 +/- 56 microns) and contained approximately six times as many B cells and eight times as many T cells as controls. Macrophages and plasma cells containing IgG and IgM were not significantly increased.
  
  
  CONCLUSIONS
  These findings extend the qualitative observations of increased follicular and lamina propria lymphoid tissue in bypassed segments of colon, and are consistent with the hypothesis of persistent antigenic stimulation of the mucosa-associated lymphoid tissue.
authors.affiliation: Department of Pathobiology, University of Connecticut, Storrs 06269-3089, USA.
authors.forename: N J
authors.initials: NJ
authors.lastname: Grant
authors.forename: H J
authors.initials: HJ
authors.lastname: Van Kruiningen
authors.forename: S
authors.initials: S
authors.lastname: Haque
authors.forename: A B
authors.initials: AB
authors.lastname: West
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D003092:Colitis / Q000209:etiology / Q000473:pathology*; D003125:Colostomy / Q000009:adverse effects*; D006627:Hirschsprung Disease / Q000601:surgery*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007223:Infant; D007413:Intestinal Mucosa / Q000473:pathology*; D008264:Macrophages / Q000473:pathology; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M