Sequence of a human E2F-4 pseudogene with shortened AGC trinucleotide repeats.
We have isolated a human genomic DNA fragment of 0.7 kb by polymerase chain reaction (PCR) using human E2F-4 gene primers. Sequence analysis indicates that this DNA comes from a processed E2F-4 pseudogene. Its sequence is 75% identical to the human E2F-4 cDNA, and bears several deletions and insertions. A 30 bp deletion shortens the 13 AGC repeats in the E2F-4 gene by 10 repeats. The sequence data also suggest that another 65 bp deletion and some small insertions may be generated by replication errors on template pairing.
Cheng
C M
CM
Department of Biology, Kaohsiung Medical College, Taiwan, Republic of China.
Chang
Y F
YF
Yuo
C Y
CY
D000595:Amino Acid Sequence; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D050704:E2F4 Transcription Factor; D006801:Humans; D008969:Molecular Sequence Data; D011544:Pseudogenes; D012091:Repetitive Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D050704:E2F4 Transcription Factor; D014157:Transcription Factors