title: Autotransfusion after total knee arthroplasty. Effects on blood cells, plasma chemistry, and whole blood rheology.
abstract: Postoperative drain blood was collected and reinfused using the ConstaVac system (Stryker, Kalamazoo, MI) in 30 patients after total knee arthroplasty. Of the total 1.1-L volume of postoperative bleeding, 60% was reinfused. No clinical complications were observed. Differences between venous blood and drain blood and between venous blood and drain blood after separate incubation were studied with respect to acidic and inflammatory effects on blood cells, plasma chemistry, and whole blood rheology. In drain blood, leukocyte and platelet counts were reduced (P < .001), probably as a result of consumption in the wound. Acidic incubation occurs in the drain container because of production of lactate from glucose, with a minimum pH at 5 hours of 7.2. The low pH caused slight but significant erythrocyte swelling (P < .01). The complement C3d indicated leukocyte activation, although of modest magnitude. Despite incubation and complement activation, maximum erythrocyte hemolysis after 24 hours of incubation was less than 1%. Drain blood showed a lower resistance against micropore filtration than venous blood (P < .001), mainly because of the reduced number of leukocytes, and remained unchanged with incubation. Although the autotransfusion system can be improved with respect to blood quality, filtered drain blood should be considered acceptable for reinfusion.
authors.affiliation: Department of Orthopedics, University of Umeå, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Dalén
authors.forename: L A
authors.initials: LA
authors.lastname: Broström
authors.forename: K G
authors.initials: KG
authors.lastname: Engström
mesh_terms: D000368:Aged; D001772:Blood Cell Count; D001773:Blood Cells / Q000737:chemistry / Q000166:cytology*; D016063:Blood Loss, Surgical; D001804:Blood Transfusion, Autologous; D001810:Blood Volume; D004906:Erythrocyte Count; D005260:Female; D005374:Filtration; D018056:Hemorheology; D006801:Humans; D007720:Knee Prosthesis; D008297:Male; D010949:Plasma / Q000737:chemistry*; D011184:Postoperative Period