Glyceraldehyde 3-phosphate-induced DNA or protein modifications severely inhibit the protein/DNA interaction.
In this study, the effect of the reducing sugar glyceraldehyde 3-phosphate on protein/DNA interaction has been investigated. Treatment with glyceraldehyde 3-phosphate of oligonucleotides recognized by various transcription factors severely inhibits protein binding. The inhibitory effect is time and dose-dependent. Treatment with glyceraldehyde 3-phosphate of the homeodomain protein TTF-1 HD has also an inhibitory effect on the interaction with DNA, again in a time and dose-dependent manner. These "in vitro" effects could have "in vivo" counterparts and therefore contribute to molecular alterations observed either when intracellular protein are exposed to high doses of reducing sugars (i.e. in diabetes) or after a long time exposure (i.e. in Gzero-arrested cells during aging).
Moro
M
M
Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, Italy.
Ceriello
A
A
Mercuri
F
F
Tell
G
G
Pellizzari
L
L
Damante
G
G
D000818:Animals; D016922:Cellular Senescence / Q000187:drug effects; D004247:DNA / Q000187:drug effects*; D003920:Diabetes Mellitus / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D005986:Glyceraldehyde 3-Phosphate / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D011485:Protein Binding / Q000187:drug effects*
D005986:Glyceraldehyde 3-Phosphate; D004247:DNA