Relationship between the release of prolactin and endothelin-1 in human decidualized endometrial cells.
The study was undertaken to investigate the interaction between the release of endothelin-1 (ET-1) and prolactin (PRL) from human decidualized endometrial cells, and the effects of transforming growth factor (TGF)-beta 1 on the release of ET-1 and PRL from human decidual cells in the early stage of pregnancy. Stromal cells derived from human endometrial tissues were cultured on a type-1 collagen membrane in serum-free medium with or without 100 nmol/l oestradiol and 1 mumol/l medroxyprogesterone acetate (MPA) for 12 days. In addition, decidual cells of early pregnancy were cultured with or without TGF-beta 1 for 48 h. PRL and ET-1 levels in the media were measured by a specific enzyme immunoassay and RIA respectively. In decidualized endometrial cells induced by ovarian steroid hormones in vitro, PRL release increased and ET-1 release decreased in a time-dependent manner. Ovarian steroid hormones significantly attenuated ET-1 release. In the decidual cells of early pregnancy, TGF-beta 1 significantly attenuated the PRL release, whereas this growth factor dose-dependently increased ET-1 release. There was a significant negative correlation between the release of PRL and that of ET-1. These results demonstrate that the regulation of PRL release is quite different from that of ET-1 release in the human decidualized endometrial cells, and suggest that TGF-beta 1 has significant effects on PRL and ET-1 release in the human decidua of early pregnancy.
Kubota
T
T
Department of Obstetrics and Gynecology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Taguchi
M
M
Kobayashi
K
K
Masuda
M
M
Aso
T
T
D000328:Adult; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D019332:Endothelin-1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011388:Prolactin / Q000378:metabolism*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D019332:Endothelin-1; D016212:Transforming Growth Factor beta; D011388:Prolactin