Serotonin 5-HT2A receptors are expressed on pyramidal cells and interneurons in the rat cortex.
The distribution of 5-HT2A receptors in rat cortex was evaluated using newly developed antibodies. Each of three antibodies tested identified an identical pattern of 5-HT2A-like immunoreactivity (5-HT2A-li) in rat cortex with 5-HT2A-li showing a widespread distribution. The majority of 5-HT2A-li cells displayed a pyramidal morphology. While a minority, some cortical neurons with a bipolar morphology displayed 5-HT2A-li as well. Dual-label fluorescence confocal microscopic studies with a 5-HT2A antibody and a mouse monoclonal antibody to parvalbumin, a marker of a subset of gamma aminobutyric acid (GABA)ergic interneurons in the cortex, demonstrated that although some cells expressing 5-HT2A-li were interneurons, most were not.
Willins
D L
DL
Department of Psychiatry, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4935, USA.
Deutch
A Y
AY
Roth
B L
BL
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D007150:Immunohistochemistry; D007395:Interneurons / Q000378:metabolism*; D008297:Male; D017966:Pyramidal Cells / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000378:metabolism*
D011985:Receptors, Serotonin