Stability and stabilization of recombinant peroxidase in reversed micelles.
Stability of recombinant peroxidase lacking carbohydrate residues on the surface of the protein molecule has been characterized in reversed micelles of Aerosol OT in octane. The enzyme stability was found to depend on the surfactant hydration degree (w0 = [H2O]/[AOT]). Residual activity after 1 h incubation dropped to zero at w0 = 7 but was 54% at w0 = 25. However, the residual activity levels at all values of hydration degree were definitely low compared to that of glycosylated wild-type horseradish peroxidase. The stability of the enzyme apparently depends on the presence of carbohydrate residues. Stabilization of recombinant peroxidase in reversed micellar system involved sugar-containing co-surfactants such as Tweens and Spans is proposed. As an example, addition of 1 mM Span 80 (1% relative to AOT concentration) increased the recombinant peroxidase stability up to that of wild-type peroxidase.
Klyachko
N L
NL
Division of Chemical Enzymology, School of Chemistry, Lomonosov Moscow State University, Russia.
Dulkis YuK
Sukhoruchenko
T A
TA
Levashov
A V
AV
D003902:Detergents; D004143:Dioctyl Sulfosuccinic Acid / Q000378:metabolism; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology*; D006031:Glycosylation; D006601:Hexoses; D007700:Kinetics; D008823:Micelles; D009844:Oligosaccharides / Q000494:pharmacology; D009195:Peroxidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011136:Polysorbates; D011994:Recombinant Proteins / Q000737:chemistry
D003902:Detergents; D006601:Hexoses; D008823:Micelles; D009844:Oligosaccharides; D011136:Polysorbates; D011994:Recombinant Proteins; C018665:sorbitan monooleate; D004143:Dioctyl Sulfosuccinic Acid; D009195:Peroxidase