Autologous versus allogeneic T cell-stimulated IL-6 production by dermal fibroblasts.
T cells adhere to human dermal fibroblasts (HDF). This cellular interaction leads to a pronounced secretion of the proinflammatory cytokines IL-6 and IL-8 via a juxtacrine stimulation induced by HDF-associated IL-1. Upon stimulation, fibroblasts express various surface proteins such as MCH-I molecules, which may interact with corresponding receptors on T cells. The present study was conducted to further investigate the mechanism of this complex interaction with regard to the secretion of IL-6 in cocultures of T cells and HDF. IL-6 was time- and dose-dependently upregulated in such cocultures. Spatial separation of the cells by microporous membranes resulted in a 90% reduction of IL-6 secretion, but when cells had limited cell contact IL-6 secretion was increased again. Allogeneic cocultures of T cells and HDF showed increased capacity of IL-6 stimulation as compared to autologous cultures. Our results suggest that MHC-I/T cell receptor interaction modulates IL-6 secretion in allogeneic and autologous cocultures.
Spörri
B
B
Department of Oral Cell Biology, University of Bern, Switzerland.
Bickel
M
M
Limat
A
A
Waelti
E R
ER
Hunziker
T
T
Wiesmann
U N
UN
D002450:Cell Communication; D002469:Cell Separation; D018920:Coculture Techniques; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000502:physiology*; D013997:Time Factors
D015850:Interleukin-6