The NAD synthetase NadE (OutB) of Bacillus subtilis is a sigma B-dependent general stress protein.
The identification of sigma B-dependent general stress proteins is a useful strategy to understand the physiological role of the unspecific stress response in Bacillus subtilis. By N-terminal sequencing of B. subtilis stress proteins Gsp38 was identified as the NAD-synthetase (NadE). NadE was previously characterized as spore outgrowth factor B (OutB) conferring a temperature-sensitive spore outgrowth defective phenotype. Transcriptional studies showed that nadE is strongly induced in response to heat, ethanol and salt stress or after starvation for glucose in a sigma B-dependent manner. Two promoters are involved in transcriptional initiation, the sigma A-dependent upstream promoter contributes to the basal level during growth, whereas the sigma B-dependent downstream promoter is induced after different stress conditions.
Antelmann
H
H
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Schmid
R
R
Hecker
M
M
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D001483:Base Sequence; D019733:Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor; D000431:Ethanol / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000502:physiology; D006358:Hot Temperature; D008025:Ligases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012329:RNA, Bacterial / Q000032:analysis; D017421:Sequence Analysis; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D014158:Transcription, Genetic / Q000235:genetics
D001426:Bacterial Proteins; D012329:RNA, Bacterial; C079011:SigB protein, Bacteria; D012808:Sigma Factor; D000431:Ethanol; D012965:Sodium Chloride; D008025:Ligases; D019733:Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor; C063522:NAD+ synthase (glutamine-hydrolysing); C080136:OutB protein, Bacillus subtilis; D005947:Glucose