Structural studies of the O-antigen polysaccharide from Escherichia coli O138.
The structure of the O-antigen polysaccharide from Escherichia coli O138 has been determined. NMR spectroscopy, together with component and methylation analyses, of native and reduced polysaccharide were the principal methods used. The sequence of the sugar residues could be determined by NOESY and heteronuclear multiple bond connectivity (HMBC) NMR experiments. It is concluded that the polysaccharide is composed of tetrasaccharide repeating units with the following structure: [structure: see text].
Linnerborg
M
M
Department of Organic Chemistry, Stockholm University, Sweden.
Weintraub
A
A
Widmalm
G
G
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification
D019081:O Antigens; D009844:Oligosaccharides