title: Repetin (Rptn), a new member of the "fused gene" subgroup within the S100 gene family encoding a murine epidermal differentiation protein.
abstract: We report the cloning and characterization of a murine epidermal differentiation gene, repetin (Rptn), exhibiting striking similarity to the genes of the intermediate filament-associated proteins profilaggrin and trichohyalin. The repetin gene consists of three exons and two introns. The first exon is short and untranslated. The deduced amino acid sequence distributed between exons II and III contains 1130 amino acids with a calculated molecular mass of 130 kDa and pI of 7.7. The amino terminus exhibits significant homology to the S100 proteins containing two calcium-binding motifs of the EF-hand type. The remainder coding sequence contains a central segment consisting of 49 tandem repeats of a 12-amino-acid sequence rich in glutamines. By fluorescence in situ hybridization the repetin gene was localized to chromosome band 3 F1-2. Expression of repetin mRNA is detectable in the stratified internal epithelia of forestomach and tongue and to a lesser degree in normal skin epidermis, where it is restricted to the differentiated suprabasal cell layers. Based on its chromosomal localization, its genomic organization, and its stage-specific expression during late epidermal differentiation, as well as on the structural features of the encoded protein, we conclude that the repetin gene represents a novel member of the "fused gene" subgroup of the S100 gene family encoding multifunctional epidermal matrix proteins.
authors.affiliation: Division of Biochemistry of Tissue Specific Regulation, Deutsches Krebsforschungszentrum, Heidelberg, Germany. p.krieg@dkfz-heidelberg.de
authors.forename: P
authors.initials: P
authors.lastname: Krieg
authors.forename: M
authors.initials: M
authors.lastname: Schuppler
authors.forename: R
authors.initials: R
authors.lastname: Koesters
authors.forename: A
authors.initials: A
authors.lastname: Mincheva
authors.forename: P
authors.initials: P
authors.lastname: Lichter
authors.forename: F
authors.initials: F
authors.lastname: Marks
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004817:Epidermis / Q000737:chemistry*; D005786:Gene Expression Regulation; D005796:Genes / Q000235:genetics; D015698:Genomic Library; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D009418:S100 Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; C108628:Rptn protein, mouse; D009418:S100 Proteins