title: Origin of pleural cells after lung transplantation: from donor or recipient?
abstract: We evaluated the change in the percentage of cells of donor origin in pleural fluid of 13 consecutive patients who underwent lung transplantation. Pleural fluid was sampled 2, 4, and 8 days after lung transplantation. DNA, which was extracted from the blood of donors and recipients and from the pleural fluid, was amplified using a polymerase chain reaction technique. The reaction products were electrophoresed, and bands indicating amplified human leukocyte antigen (HLA)-DR alleles were quantified by determining the area under the curve (AUC) by a densitometric analysis. HLA-DR alleles, which were present only in recipient cells (recipient allele), were analyzed and compared to HLA-DR alleles that were present only in donor cells (donor allele). A dilution study was first performed to provide a standard curve relating the percentage of donor and recipient cells in a mixture to their AUC. The AUC of the recipient alleles did not change significantly over the first 8 postoperative days. The AUC of the donor alleles was less on postoperative days 4 and 8 than on day 2 (p<0.05). The donor allele AUC on day 8 was <20% of the shared allele AUC, corresponding to <1% of all cells by the dilution study. We conclude that donor cells are rapidly cleared from the pleural space after lung transplantation, with <1% of cells of donor origin by postoperative day 8.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Medical University of South Carolina, Charleston 29425-2220, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Judson
authors.forename: S A
authors.initials: SA
authors.lastname: Sahn
authors.forename: A B
authors.initials: AB
authors.lastname: Hahn
mesh_terms: D000328:Adult; D000483:Alleles; D002465:Cell Movement; D004247:DNA / Q000032:analysis; D005260:Female; D006684:HLA-DR Antigens / Q000235:genetics; D016041:Heart-Lung Transplantation / Q000473:pathology; D006801:Humans; D016040:Lung Transplantation / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010994:Pleura / Q000166:cytology*; D010996:Pleural Effusion / Q000166:cytology*; D016133:Polymerase Chain Reaction; D011184:Postoperative Period; D013997:Time Factors; D014019:Tissue Donors
chemical_list: D006684:HLA-DR Antigens; D004247:DNA