[Human melanoma-specific peptide antigens recognized by HLA-A2 restricted tumor-infiltrating lymphocytes].
OBJECTIVE
HLA-A2-associated peptides were extracted from human melanoma cell lines and used to study human melanoma-specific peptide antigens for HLA-A2-restricted tumor-infiltrating lymphocyte (TIL).


METHODS
TILS were derived from the peripheral lymph nodes of HLA-A2+ human melanoma patients, HLA-A2 molecules were purified from the melanoma cell lines by immunoaffinity column chromatography of detergent-solubilized cell pellets. Peptides bound to the HLA-A2 molecules were acid eluted and fractionated by reversed phase RP-HPLC. Individual fractions were assessed for their ability to reconstitute melanoma-specific epitopes by adding to the HLA-A2+ Ag-processing mutant cell, T2.


RESULTS
These TIL lysed HLA-A2+ autologous and allogeneic melanomas, but not the HLA-A2- melanomas. They also did not lyse the HLA-A2+ non-melanoma cell lines. The RP-HPLC separations of reconstituting fractions revealed three peaks (fractions) of reconstitution.


CONCLUSIONS
These results showed that peptides derived from three active fractions were related to human melanoma-specific peptide antigens recognized by HLA-A2-restricted TIL.
Ge
H
H
Shanghai Institute of Immunology, Shanghai Second Medical University.
Bao
Z
Z
D000951:Antigens, Neoplasm / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D015789:HLA-A2 Antigen / Q000276:immunology*; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008545:Melanoma / Q000276:immunology / Q000473:pathology; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D015789:HLA-A2 Antigen; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins