title: Macular holes: migratory gaps and vitreous as obstacles to glial closure.
abstract: OBJECTIVE
  Retinal glia may play an important role in the closure of macular holes. This in vitro study examines whether and how the specific pathoanatomy, including foveal eversion and foveal vitreous, may interfere with glial closure of macular holes.
  
  
  METHODS
  Culture dishes used to grow glial cells were modified by the placement of slopes, vertical steps, and gaps to mimic the in vivo migratory surface in and surrounding macular holes. In separate experiments, defects were made in a rodent glial monolayer. These defects were exposed to hyaluronic acid (HA) and to rabbit (RV) and bovine (BV) vitreous gel. The migratory behavior and completeness of closure of defects were compared to controls.
  
  
  RESULTS
  As expected, glial cells migrated further and in greater numbers on a smooth surface. Slopes and steps were moderate obstacles to migration; gaps in the surface were absolute obstacles. HA modified the pattern of adhesion of cells at the bottom of defects. Defects in the glial monolayer were repaired in 5-7 days. Compared to these controls, repair was inhibited by 11% (n.s.), 28% (P = 0.02), and 58% (P = 0.004) after direct exposure of defects to HA, RV and BV, respectively.
  
  
  CONCLUSIONS
  The elevated and everted margins of macular holes represent slope, step, and gap-like obstacles to the migration of glial cells and hence to the healing of defects. The defect allows extension of extracellular matrix into it and the subretinal space. Our results indicate that gaps in the migratory surface caused and aggravated by eversion and the presence of vitreous present obstacles to glial migration and closure of macular holes.
authors.affiliation: Department of Ophthalmoloy, Columbia University, New York, USA.
authors.forename: H D
authors.initials: HD
authors.lastname: Schubert
authors.forename: K
authors.initials: K
authors.lastname: Kuang
authors.forename: F
authors.initials: F
authors.lastname: Kang
authors.forename: M W
authors.initials: MW
authors.lastname: Head
authors.forename: J
authors.initials: J
authors.lastname: Fischbarg
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002455:Cell Division; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D006820:Hyaluronic Acid / Q000494:pharmacology; D009457:Neuroglia / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D012167:Retinal Perforations / Q000473:pathology / Q000503:physiopathology*; D014822:Vitreous Body / Q000503:physiopathology*; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: D006820:Hyaluronic Acid