title: KpnAI, a new type I restriction-modification system in Klebsiella pneumoniae.
abstract: The KpnAI restriction-modification (R-M) system has been identified in Klebsiella pneumoniae strain M5a1. The restriction gene of KpnAI was first cloned into pBR322 using an r-m+ M5a1 derivative and phage SBS for screening. Subsequently, an adjacent DNA fragment showing modification activity was cloned into pUC19. A total of 7.2 kb DNA sequencing data revealed three open reading frames, corresponding to hsdR, hsdM and hsdS genes of type I R-M systems. The predicted hsdR, hsdM and hsdS-coded peptides shared 95%, 98% and 44% identity, respectively, with the corresponding peptides of the recently identified StySBLI system, a prototype of the type ID family. This high homology suggests that KpnAI is also a member of the type ID family. The KpnAI system seems to be the first type I system identified in Klebsiella species.
authors.affiliation: Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda, CA 92350, USA.
authors.forename: N S
authors.initials: NS
authors.lastname: Lee
authors.forename: O
authors.initials: O
authors.lastname: Rutebuka
authors.forename: T
authors.initials: T
authors.lastname: Arakawa
authors.forename: T A
authors.initials: TA
authors.lastname: Bickle
authors.forename: J
authors.initials: J
authors.lastname: Ryu
mesh_terms: D003001:Cloning, Molecular; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics*; D004926:Escherichia coli; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D010455:Peptides / Q000737:chemistry; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics
chemical_list: D015280:DNA Restriction-Modification Enzymes; D010455:Peptides; C072994:EcoR124 modification methylase type I; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)