Protein half-lives of calmodulin and the plasma membrane Ca-ATPase in rat brain.
We report the half-lives for two proteins involved in the regulation of intracellular calcium in the brain: the plasma membrane Ca-ATPase and its regulatory protein, calmodulin. [14C]-labeled leucine was injected into seven month old adult Fischer 344 rats and the time-dependent appearance and loss of radioactivity was monitored in both the serum and proteins from the brains of rats sacrificed from 4 hours to 13 days after injection. Experimental data obtained for calmodulin and the plasma membrane Ca-ATPase are best described by theoretical curves accounting for leucine reutilization that assume apparent half-lives of 18 (+/-2) hours and 12 (+/-1) days, respectively.
Ferrington
D A
DA
Department of Biochemistry, University of Kansas, Lawrence 66045, USA.
Chen
X
X
Krainev
A G
AG
Michaelis
E K
EK
Bigelow
D J
DJ
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002147:Calmodulin / Q000096:biosynthesis / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D006207:Half-Life; D007930:Leucine / Q000378:metabolism; D008297:Male; D011865:Radioisotope Dilution Technique; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors
D002147:Calmodulin; D002250:Carbon Radioisotopes; D007930:Leucine