title: The polymerase chain reaction for the identification of different species of Mycobacterium.
abstract: The polymerase chain reaction (PCR) was performed to identify reference and field strains of mycobacteria. PCR was able to identify all the reference strains of the Mycobacterium genus and sub-divide them into no Tuberculosis-no Avium complex, Tuberculosis complex and, partially, Avium complex. The primers used for the last recognised only some strains of the different types of M. avium and M. intracellulare. A number of field strains were identified as belonging to the Mycobacterium genus, but further research is required for a complete sub-division into Tuberculosis complex and Avium complex.
authors.affiliation: Istituto di Patologia e Igiene Veterinaria, Facoltà Medicina Veterinaria, Università di Padova, Agripolis, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Valente
authors.forename: S
authors.initials: S
authors.lastname: Demas
authors.forename: S
authors.initials: S
authors.lastname: Scarso
authors.forename: V
authors.initials: V
authors.lastname: Cuteri
authors.forename: L
authors.initials: L
authors.lastname: Job
authors.forename: S
authors.initials: S
authors.lastname: Marangon
authors.forename: L
authors.initials: L
authors.lastname: Dalla Valle
authors.forename: F M
authors.initials: FM
authors.lastname: Cancellotti
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015269:Mycobacterium avium Complex / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial