Molecular mechanisms of immunomodulating effect of chorionic gonadotropin on T- and B-lymphocytes of intact spleen.
Chorionic gonadotropin (CG) was injected into a short-term macroculture of fractionated splenocytes of female since F1(CBA x C57BL/6) in concentrations of 10 or 50 IU/ml. The regulatory effects of CG were assessed in a syngeneic transfer system by the number of antibody-producing cells (APC) of the recipients CG at a dose of 10 IU/ml selectively activated the intact B-lymphocytes and failed to affect the functional activity of T-cells. The effects of low hormone doses were associated with the inositide system of secondary messengers and a decrease in the level of cAMP during the influence of the hormone. The dose of 50 IU/ml also suppressed cAMP in B-lymphocytes but the number of APC did not change and failed to depend on the inhibitor of inositol-1-monophosphatase. The high dose of CG significantly increased the level of cAMP in the T-lymphocytes, and this was associated with a significant suppression of the functional activity of these cells. Different effects of the high and low hormone doses on different populations of the spleen immunocompetent cells found in vitro were also confirmed in vivo on ovariectomized donor females.
Shirshev
S V
SV
Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, Perm, Russia.
D000276:Adjuvants, Immunologic / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010052:Ovariectomy; D015398:Signal Transduction; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D000276:Adjuvants, Immunologic; D002121:Calcium Channel Blockers; D006063:Chorionic Gonadotropin