title: Full-scale 'naïve' human antibody repertoires assembled from VH and VL variable regions.
abstract: Very large 'naïve' human antibody repertoires have been obtained from RT-PCR cloned VH and VL variable regions. They are used as starting material for the assembly of medium sized combinatorial libraries or so called multicombinatorial libraries. In nonimmunized individuals immunoglobulin messenger RNAs are poorly expressed, which can be a serious limitation for cloning efficiency. To overcome this problem two complementary strategies have been used: a nonspecific polyclonal activation of B cells, and a secondary PCR amplification technique to ensure recovery of Ig messengers in large amount and without introducing any bias.
authors.affiliation: Pasteur Merieux Connaught, Laboratoire de Technologie Combinatoire, Marcy l'Etoile, France.
authors.forename: R
authors.initials: R
authors.lastname: Sodoyer
authors.forename: I
authors.initials: I
authors.lastname: Peubez
authors.forename: C
authors.initials: C
authors.lastname: Pion
authors.forename: J
authors.initials: J
authors.lastname: Dubayle
authors.forename: P
authors.initials: P
authors.lastname: Jacquemot
authors.forename: F
authors.initials: F
authors.lastname: Geoffroy
authors.forename: J
authors.initials: J
authors.lastname: Even
authors.forename: L
authors.initials: L
authors.lastname: Aujame
mesh_terms: D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D000906:Antibodies; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region