title: Influenza virus neutralizing antibodies and IgG isotype profiles after immunization of mice with influenza A subunit vaccine using various adjuvants.
abstract: The influence of various adjuvants on the development of influenza virus neutralizing antibodies and distribution of anti-influenza virus IgG isotypes after immunization of mice with influenza A (H3N2) subunit vaccine was investigated. Serum titres of influenza virus neutralizing antibodies and titres of influenza specific IgG isotypes were determined by a neutralization enzyme immunoassay (N-EIA) and a cell-associated antigen enzyme immunoassay (CA-EIA), respectively. Serum antibody titres as measured by the two tests correlated highly (r = 0.82; P < 0.001). N-EIA titres were enhanced by 38- and 34-fold, when L180.5/RaLPS and FCA, respectively, were administered with 1 microgram of vaccine. The adjuvants Q-VAC, L180.5 [W/O/W], L180.5 alone and Montanide ISA 740 were only moderately or not effective in enhancing the immune response to the 1 microgram dose of vaccine. The Q-VAC and L180.5/RaLPS adjuvants favoured IgG2a and IgG2b isotype responses to influenza compared to the other adjuvants. We suggest that N-EIA and CA-EIA may be valuable tools to monitor the effects of adjuvants on the neutralizing antibody and antibody isotype responses after influenza vaccination.
authors.affiliation: Regional Public Health Laboratory, Groningen, The Netherlands.
authors.forename: C A
authors.initials: CA
authors.lastname: Benne
authors.forename: M
authors.initials: M
authors.lastname: Harmsen
authors.forename: W
authors.initials: W
authors.lastname: van der Graaff
authors.forename: A F
authors.initials: AF
authors.lastname: Verheul
authors.forename: H
authors.initials: H
authors.lastname: Snippe
authors.forename: C A
authors.initials: CA
authors.lastname: Kraaijeveld
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood / Q000187:drug effects / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D000882:Haplorhini; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood / Q000145:classification; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis* / Q000097:blood; D009980:Influenza A virus / Q000737:chemistry / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D007668:Kidney / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D009976:Orthomyxoviridae Infections / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007252:Influenza Vaccines