title: Mapping the distribution of outer hair cell voltage-dependent conductances by electrical amputation.
abstract: The mammalian outer hair cell (OHC) functions not only as sensory receptor, but also as mechanical effector; this unique union is believed to enhance our ability to discriminate among acoustic frequencies, especially in the kilohertz range. An electrical technique designed to isolate restricted portions of the plasma membrane was used to map the distribution of voltage-dependent conductances along the cylindrical extent of the cell. We show that three voltage-dependent currents, outward K, I(K,n), and I(Ca) are localized to the basal, synaptic pole of the OHC. Previously we showed that the lateral membrane of the OHC harbors a dense population of voltage sensor-motor elements responsible for OHC motility. This segregation of membrane molecules may have important implications for auditory function. The distribution of OHC conductances will influence the cable properties of the cell, thereby potentially controlling the voltage magnitudes experienced by the motility voltage sensors in the lateral membrane, and thus the output of the "cochlear amplifier."
authors.affiliation: Section of Otolaryngology, Yale University School of Medicine, New Haven, Connecticut 06510, USA. joseph.santos-sacchi@yale.edu
authors.forename: J
authors.initials: J
authors.lastname: Santos-Sacchi
authors.forename: G J
authors.initials: GJ
authors.lastname: Huang
authors.forename: M
authors.initials: M
authors.lastname: Wu
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002465:Cell Movement; D004553:Electric Conductivity; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008954:Models, Biological; D018408:Patch-Clamp Techniques