Comparison of peripheral blood CD34+ concentration, colony-forming units granulocyte-macrophage and mononuclear cells in leukapheresed product for the prediction of peripheral blood CD34+ cell yield harvest.
The aim of this study was to evaluate reliability of parameters which may be used to guide peripheral stem cell harvests in cancer patients prior to myeloablative chemotherapy. Each leukapheresed product was analysed for CD34-positive (CD34+) cell count, mononuclear cell (MNC) count and the number of colony-forming units granulocyte-macrophage (CFU-GM). Each patient's peripheral blood (PB) taken before leukapheresis was analysed for CD34+ concentration. We evaluated whether the CD34+ yield from leukapheresis correlated with any of the three parameters. A total of 119 procedures were performed in 33 patients. The yield of CD34+ cells by leukapheresis correlated weakly but significantly with the peripheral blood CD34+ cell count (R = 0.4 P < 0.05), the MNC cells (R = 0.4, P < 0.05), and CFU-GM (R = 0.4, P < 0.05). When a PB CD34+ count of 50 x 10(6)/L was used as a cut-off value, the accuracy for prediction of adequate leukapheresis (> 1 x 10(6) CD34+ cells/kg) was 78%.
Tao
M
M
Department of Medical Oncology, Singapore General Hospital, Singapore.
Teo
C P
CP
Ngai
J
J
Ong
Y W
YW
Ang
P T
PT
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000097:blood*; D005260:Female; D006098:Granulocytes; D006412:Hematopoietic Stem Cells; D006801:Humans; D007937:Leukapheresis; D007958:Leukocyte Count; D008264:Macrophages; D008297:Male; D008875:Middle Aged; D009000:Monocytes; D012189:Retrospective Studies; D013997:Time Factors
D018952:Antigens, CD34