[Study on some susceptible genes of systemic lupus erythematosus in Han nationality of China].
In order to explore the allelic polymorphism of HLA-DR and TNF B loci and susceptibility to systemic lupus erythematosus (SLE) in the Han nationality of northern China with the aid of methods of polymerase chain reaction/sequence specific primers (PCR/SSP) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) respectively. The findings from a case-control study on 151 blood samples (45 from the cases and 106 from the controls) indicated that there were significantly higher frequency of DR2 (P < 0.05, RR = 1.56) and DR3 (P < 0.01, RR = 2.69), which represent candidate susceptible genes or useful markers for SLE. The DR5 allele in the samples (P < 0.05, RR = 0.43) might be an antagonistic or protective allele, or a marker for such allele. The frequency of TNF B * 1 and TNF B * 2 alleles was investigated in 45 SLE patients and 80 healthy controls and it was found that the frequency of TNF B * 2 allele was significantly higher in the patient group (P < 0.05, RR = 1.84). It might also be a suspicious susceptible allele or a marker for such allele. The frequency of HLA polymorphisms in various clinical/immunological subsets of our patient population was also determined. Clinical findings used include plasma SC5b-9 level, SSA, SSB, Sm, RNP, ANA antibodies, and SLE complications (SLE nephritis, pneumonia & encephalopathy). It turned out that there was a positive association between HLA-DR2 allele and SLE nephritis (P < 0.05, RR = 1.32).
Zhang
J
J
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Zhou
F
F
Ai
R
R
D000483:Alleles; D044466:Asian People; D002681:China; D004198:Disease Susceptibility; D005787:Gene Frequency; D005819:Genetic Markers; D015802:HLA-DR2 Antigen / Q000235:genetics*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008233:Lymphotoxin-alpha / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D005819:Genetic Markers; D015802:HLA-DR2 Antigen; D008233:Lymphotoxin-alpha