title: [Changes in tissue and blood polyamines during N-butyl-N-(4-hydroxybutyl) nitrosamine-induced bladder carcinogenesis in rats].
abstract: BACKGROUND
  Polyamine are recognized as cell growth factors. We studied in order to determine whether alterations in the levels of tissue and blood polyamines were useful biochemical markers for bladder tumor.
  
  
  METHODS
  The concentrations of three polyamines, diamine, spermidine and spermine, in urinary bladder and blood were determined during N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)-induced bladder carcinogenesis in male F344 rats. At 5 weeks of age, rats were given 0.05% BBN in the drinking water for 20 weeks.
  
  
  RESULTS
  BBN induced bladder hyperplasia in 4 of 5 rats at 8 weeks, papillomas in 2 of 5 rats at 12 weeks, and transitional cell carcinoma in all the rats by 20 weeks. The levels of total polyamine in both bladder and blood of the rats during 12-20 weeks were significantly higher than those of the control animals given water alone. The elevation of total polyamine was mainly due to the increase of spermidine of the three polyamines, which was coincident with the incidence of bladder tumors.
  
  
  CONCLUSIONS
  The results indicated that the polyamines are excellent biochemical markers for bladder tumors.
authors.affiliation: Department of Urology, Gifu Prefectural Gifu Hospital.
authors.forename: M
authors.initials: M
authors.lastname: Taniguchi
authors.forename: K
authors.initials: K
authors.lastname: Minoshima
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
authors.forename: S
authors.initials: S
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Deguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawada
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: A
authors.initials: A
authors.lastname: Hara
mesh_terms: D000818:Animals; D002085:Butylhydroxybutylnitrosamine / Q000031:analogs & derivatives; D002273:Carcinogens; D008297:Male; D011073:Polyamines / Q000097:blood*; D051381:Rats; D011916:Rats, Inbred F344; D001749:Urinary Bladder Neoplasms / Q000097:blood* / Q000139:chemically induced*
chemical_list: D002273:Carcinogens; D011073:Polyamines; D002085:Butylhydroxybutylnitrosamine; C010723:N-ethyl-N-(4-hydroxybutyl)nitrosamine