Mediation of prostaglandin f2 alpha-induced ocular surface hyperemia by sensory nerves in rabbits.
OBJECTIVE
Prostaglandin F2 alpha (PGF2 alpha) and several other prostaglandins cause surface hyperemia of the eye when applied topically. The purpose of the present study was to investigate the role of sensory nerves in prostaglandin-induced ocular hyperemia.


METHODS
The ophthalmic branch of the trigeminal nerve was unilaterally electrocoagulated in rabbits using a stereotaxic technique. Two weeks later the animals received bilaterally one drop of PGF2 alpha-isopropyl ester (PGF2 alpha-IE) at a dose of 1 microgram/ eye. The blood flow to the different parts of the eye was measured with the radioactively labeled microsphere technique before, 30 and 60 min after treatment with PGF2 alpha-IE.


RESULTS
In the control eyes with intact sensory innervation, PGF2 alpha-IE caused a marked increase in blood flow to the surface structures of the eye (conjunctiva, anterior sclera, eyelids and nictitating membrane) and the iris, and a moderate increase in the blood flow to the ciliary processes, but no increase in the choroidal blood flow. In the denervated eyes the increase in blood flow to the surface structures was almost completely abolished, and there was also a tendency toward less increase in blood flow in the anterior uvea.


CONCLUSIONS
These results indicate that PGF2 alpha-induced ocular surface hyperemia, at least at the early stage, is mediated to a large extent by sensory nerves.
Astin
M
M
Glaucoma Research Laboratories, Pharmacia & Upjohn, Uppsala, Sweden.
Stjernschantz
J
J
D000818:Animals; D003714:Denervation; D015237:Dinoprost; D005123:Eye / Q000098:blood supply* / Q000294:innervation*; D005260:Female; D006940:Hyperemia / Q000139:chemically induced* / Q000503:physiopathology; D008297:Male; D008863:Microspheres; D009475:Neurons, Afferent / Q000502:physiology*; D011817:Rabbits; D012039:Regional Blood Flow
D015237:Dinoprost