Cloning and expression of the Borrelia burgdorferi lon gene.
The ATP-dependent protease Lon (La) of Escherichia coli degrades abnormal proteins and is involved in the regulation of capsular polysaccharide synthesis. In addition, mutations in the E. coli lon gene suppress temperature-sensitive mutations in other genes. The lon gene of Borrelia burgdorferi, encoding a homolog of the Lon protease, has been cloned and sequenced. The gene encodes a protein of 806 amino acids. The deduced amino acid sequence of the B. burgdorferi Lon protease shares substantial sequence identity with those of other known Lon proteases. The transcription start point of the B. burgdorferi lon gene was identified by primer extension analysis and the potential promoter did not show similarities to the consensus heat-shock promoter in E. coli. The 5'-end of the B. burgdorferi lon gene appears to suppress the temperature-sensitive phenotype of an E. coli lpxA mutant.
Cloud
J L
JL
Magic Valley Regional Medical Center, Twin Falls, ID 83301, USA.
Marconi
R T
RT
Eggers
C H
CH
Garon
C F
CF
Tilly
K
K
Samuels
D S
DS
D049069:ATP-Dependent Proteases; D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D011401:Promoter Regions, Genetic; D049070:Protease La; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D011135:Polysaccharides, Bacterial; D011994:Recombinant Proteins; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La; D000251:Adenosine Triphosphatases