Expression of PEP carboxylase from Escherichia coli complements the phenotypic effects of pyruvate carboxylase mutations in Saccharomyces cerevisiae.
We investigated the effects of the expression of the Escherichia coli ppc gene encoding PEP carboxylase in Saccharomyces cerevisiae mutants devoid of pyruvate carboxylase. Functional expression of the ppc gene restored the ability of the yeast mutants to grow in glucose-ammonium medium. Growth yield in this medium was the same in the transformed yeast than in the wild type although the growth rate of the transformed yeast was slower. Growth in pyruvate was slowed down in the transformed strain, likely due to a futile cycle produced by the simultaneous action of PEP carboxykinase and PEP carboxylase.
Flores
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Instituto de Investigaciones Biomédicas, Unidad de Bioquimica y Genética de Levaduras, Madrid, Spain.
Gancedo
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D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005816:Genetic Complementation Test; D005822:Genetic Vectors / Q000235:genetics; D016296:Mutagenesis; D010641:Phenotype; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D011766:Pyruvate Carboxylase / Q000235:genetics* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D010729:Phosphoenolpyruvate Carboxykinase (GTP); D011766:Pyruvate Carboxylase