Analytical electron microscopy as a powerful tool in plant cell biology: examples using electron energy loss spectroscopy and X-ray microanalysis.
Energy filtering transmission electron microscopy in combination with energy dispersive X-ray analysis (EDX) and quantumchemical calculations opens new possibilities for elemental and bone analysis at the ultrastructural level. The possibilities and limitations of these methods, applied to botanical samples, are discussed and some examples are given. Ca-oxalate crystals in plant cell vacuoles show a specific C K-edge in the electron energy loss spectrum (EELS), which allows a more reliable identification than light microscopical or cytochemical methods. In some dicots crystalline inclusions can be observed in different cell compartments, which are identified as silicon dioxide or calcium silicate by the fine structure of the Si L2,3-edge. Their formation is discussed on the basis of EEL-spectra and quantumchemical calculations. Examples concerning heavy metal detoxification are given for some tolerant plants. In Minuartia Zn is bound as Zn-silicate in cell walls; Armeria accumulates Cu in leaf idioblasts by chelation with phenolic compounds and Cd is precipitated as CdS/phytochelatin-complexes in tomato.
Lichtenberger
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Institut für Pflanzenbiochemie, Halle (Saale)/Germany).
Neumann
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D017610:Calcium Compounds / Q000032:analysis; D002129:Calcium Oxalate / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D002614:Chelating Agents; D004577:Electron Probe Microanalysis / Q000379:methods*; D005978:Glutathione; D062385:Hydroxybenzoates; D002479:Inclusion Bodies / Q000737:chemistry; D008667:Metalloproteins / Q000032:analysis; D019216:Metals, Heavy / Q000032:analysis*; D008854:Microscopy, Electron / Q000379:methods*; D054811:Phytochelatins; D010940:Plant Proteins / Q000032:analysis; D010944:Plants / Q000737:chemistry*; D011789:Quantum Theory; D017640:Silicates / Q000032:analysis; D012822:Silicon Dioxide / Q000032:analysis; D013057:Spectrum Analysis / Q000379:methods*; D014617:Vacuoles / Q000737:chemistry; D017967:Zinc Compounds / Q000032:analysis
D017610:Calcium Compounds; D002614:Chelating Agents; D062385:Hydroxybenzoates; D008667:Metalloproteins; D019216:Metals, Heavy; D010940:Plant Proteins; D017640:Silicates; D017967:Zinc Compounds; D002129:Calcium Oxalate; D012822:Silicon Dioxide; D054811:Phytochelatins; D005978:Glutathione; C031293:calcium silicate; C029500:zinc silicate