[Alteration of p15 and p16 gene in gastric cancer].
We analyzed p15 and p16 gene alterations in gastric cancer. Only MKN45 showed both homozygous deletions but other cell lines and all of tumor specimens did not show any alterations. Using RT-PCR analysis, decreased or no expression of the p16 gene was found in 1 of 7 cell lines (except MKN45) (14.2%) and in 8 of 20 tumors (40%), whereas no abnormalities of p15 gene expression were found. These results suggest that the p16 gene may correlate with tumorigenesis and tumor expansion due to decrease or loss of gene products in gastric cancer.
Fushida
S
S
Second Dept. of Surgery, School of Medicine, Kanazawa University.
Yonemura
Y
Y
Kawamura
T
T
Nojima
N
N
Satoh
T
T
Fujita
H
H
Michiwa
Y
Y
Fujimura
T
T
Nishimura
G
G
Miwa
K
K
Miyazaki
I
I
D000230:Adenocarcinoma / Q000235:genetics; D018196:Carcinoma, Adenosquamous / Q000235:genetics; D018279:Carcinoma, Signet Ring Cell / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D017931:DNA Primers; D016147:Genes, Tumor Suppressor; D006801:Humans; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000235:genetics*; D025521:Tumor Suppressor Proteins
C493966:CDKN2B protein, human; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D017931:DNA Primers; D025521:Tumor Suppressor Proteins