Profiles of fragments after pulsed-field gel electrophoresis of cleaved genomic DNA from strains of Taylorella equigenitalis isolated from horses in Norway.
The genomic DNA of eight strains of Taylorella equigenitalis, isolated from seven Norwegian Trotters and a Norwegian pony with contagious equine metritis in Norway, was examined by pulsed-field gel electrophoresis after separate digestions with two restriction enzymes, namely, ApaI and NotI. The respective electrophoretic profiles of the fragments were essentially identical but differed from those of T. equigenitalis NCTC11184T and Kentucky 188. They also exhibited slight differences from profiles obtained from Japanese isolates. These results may possibly suggest a common genotype and a common source of infection for all these eight isolates in Norway.
Matsuda
M
M
Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Sagamihara, Japan.
Miyazawa
T
T
Ishida
Y
Y
Moore
J E
JE
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004716:Endometritis / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D005838:Genotype; D006190:Haemophilus / Q000145:classification / Q000235:genetics*; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D009664:Norway
D004269:DNA, Bacterial