Calibration of clot-based, coagulation factor assays using a first-order exponential equation and the method of nonlinear least squares.
A nonlinear mathematical model has been developed which relates the time to calcium-initiated formation of a detectable clot in citrated, factor-deficient plasma to the clotting activity of the factor deficient in the plasma. The data of clot-based factor assays fit well the exponential expression of this scheme, which can be used conveniently in the clinical laboratory to calibrate the clotting activity of individual coagulation factors in samples of citrated plasma. Aside from its clinical utility, the model may prompt the formulation of testable hypotheses not otherwise prompted by the existing, empiric approach to the quantitation of coagulation factor activity.
Retzinger
G S
GS
Department of Pathology, University of Cincinnati, OH 45267-0529, USA.
D001681:Biological Assay / Q000379:methods*; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000032:analysis*; D006801:Humans; D008962:Models, Theoretical
D001779:Blood Coagulation Factors