title: Meat allergy: III--Proteins involved and cross-reactivity between different animal species.
abstract: BACKGROUND
  Although relatively infrequent, meat allergy represents a serious problem for children both because it is generally associated with intolerance to other protein sources and because of the suggested role of meat in stimulating the gastrointestinal development during weaning.
  
  
  OBJECTIVE
  With these considerations, the aim of our work was to improve biological-biochemical knowledge of meat allergy.
  
  
  METHODS
  This study was performed using in vivo skin prick test (SPT) and in vitro (electrophoresis associated with the immunoblotting technique) tests.
  
  
  RESULTS
  Bovine serum albumin (BSA) and actin were the proteins most frequently involved in binding with the circulating IgE. BSA involvement was confirmed by SPT; the high number of positive responses observed with actin in immunoblotting was not confirmed by SPT data. Cross-reactivity between serum albumins from different animal species was demonstrated. Our studies show that in this group of children, the correspondence between the percentage of sequence identity (phylogenetic similarity) and the number of positive responses was surprisingly high.
  
  
  CONCLUSIONS
  Although further studies are necessary, the data reported here provide new biochemical data on meat allergy.
authors.affiliation: Laboratory of Toxicology, Institute of Pharmacological Sciences, University of Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Restani
authors.forename: A
authors.initials: A
authors.lastname: Fiocchi
authors.forename: B
authors.initials: B
authors.lastname: Beretta
authors.forename: T
authors.initials: T
authors.lastname: Velonà
authors.forename: M
authors.initials: M
authors.lastname: Giovannini
authors.forename: C L
authors.initials: CL
authors.lastname: Galli
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D000941:Antigens / Q000276:immunology*; D002417:Cattle; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005512:Food Hypersensitivity; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007223:Infant; D008297:Male; D008460:Meat; D011506:Proteins / Q000276:immunology*; D012709:Serum Albumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000378:metabolism; D012882:Skin Tests; D013045:Species Specificity
chemical_list: D000199:Actins; D000941:Antigens; D011506:Proteins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E