[Regulative effects of cytokines on the expression of cell adhesion molecules on human mesangial cells].
OBJECTIVE
To study the regulative effects of rhIL-1 beta or rhTNF-alpha on the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on human mesangial cells (HMC).


METHODS
At the 4th, 8th, 16th and 32nd hours after stimulation by rhIL-1 beta (25 ng/ml) or rhTNF alpha (100 ng/ml), the mRNA expression of ICAM-1 and VCAM-1 on HMC was determined with Northern blot, and their protein expression was also tested with cell ELISA analysis.


RESULTS
mRNA and protein of both ICAM-1 and VCAM-1 were only basically expressed on HMC at very low levels in the control groups without stimulation. After stimulation by rhIL-1 beta or rhTNF alpha, however, their expression was markedly upregulated. The maximal mRNA expression level of ICAM-1 stimulated by rhTNF alpha was present at the 8th hour, but in the remaining groups the maximal mRNA expression levels of ICAM-1 or VCAM-1 were all present at the 4th hour. Compared with control groups, ICAM-1 protein expression was significantly increased from the 4th hour after rhIL-1 beta stimulation (P < 0.05), but in the remaining groups ICAM-1 or VCAM-1 protein expression was significantly raised from the 8th hour after stimulation (P < 0.001).


CONCLUSIONS
These results suggest that upregulated expression of ICAM-1 and VCAM-1 by inflammatory cytokines IL-1 beta and TNF alpha may play a pathogenic role in glomerulonephritis.
Yang
B
B
Division of Nephrology, First Hospital, Beijing Medical University.
Shen
Y
Y
Ma
L
L
D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology*; D053583:Interleukin-1beta; D010446:Peptide Fragments / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
D007375:Interleukin-1; D053583:Interleukin-1beta; D010446:Peptide Fragments; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; C052994:interleukin-1beta (163-171); D018799:Intercellular Adhesion Molecule-1