title: Calbindin and parvalbumin are early markers of non-mitotically regenerating hair cells in the bullfrog vestibular otolith organs.
abstract: Earlier studies have demonstrated hair cell regeneration in the absence of cell proliferation, and suggested that supporting cells could phenotypically convert into hair cells following hair cell loss. Because calcium-binding proteins are involved in gene up-regulation, cell growth, and cell differentiation, we wished to determine if these proteins were up-regulated in scar formations and regenerating hair cells following gentamicin treatment. Calbindin and parvalbumin immunolabeling was examined in control or gentamicin-treated (GT) bullfrog saccular and utricular explants cultured for 3 days in amphibian culture medium or amphibian culture medium supplemented with aphidicolin, a blocker of nuclear DNA replication in eukaryotic cells. In control cultures, calbindin and parvalbumin immunolabeled the hair bundles and, less intensely, the cell bodies of mature hair cells. In GT or mitotically-blocked GT (MBGT) cultures, calbindin and parvalbumin immunolabeling was also seen in the hair bundles, cuticular plates, and cell bodies of hair cells with immature hair bundles. Thus, these antigens were useful markers for both normal and regenerating hair cells. Supporting cell immunolabeling was not seen in control cultures nor in the majority of supporting cells in GT cultures. In MBGT cultures, calbindin and parvalbumin immunolabeling was up-regulated in the cytosol of single supporting cells participating in scar formations and in supporting cells with hair cell-like characteristics. These data provide further evidence that non-mitotic hair cell regeneration in cultures can be accomplished by the conversion of supporting cells into hair cells.
authors.affiliation: R. S. Dow Neurological Sciences Institute, Legacy Good Samaritan Hospital, Portland, OR 97209, USA.
authors.forename: P S
authors.initials: PS
authors.lastname: Steyger
authors.forename: M
authors.initials: M
authors.lastname: Burton
authors.forename: J R
authors.initials: JR
authors.lastname: Hawkins
authors.forename: N R
authors.initials: NR
authors.lastname: Schuff
authors.forename: R A
authors.initials: RA
authors.lastname: Baird
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D015415:Biomarkers; D064026:Calbindins; D005839:Gentamicins / Q000633:toxicity; D018069:Hair Cells, Vestibular / Q000502:physiology*; D007150:Immunohistochemistry; D008938:Mitosis / Q000187:drug effects; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009924:Organ Culture Techniques; D010037:Otolithic Membrane / Q000166:cytology / Q000502:physiology*; D010320:Parvalbumins / Q000378:metabolism*; D010590:Phalloidine / Q000378:metabolism; D011892:Rana catesbeiana; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012444:Saccule and Utricle / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D015415:Biomarkers; D064026:Calbindins; D005839:Gentamicins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D010590:Phalloidine