Interleukin-10 stimulation of endogenous nitric oxide release from human saphenous veins diminishes immunocyte adherence.
Interleukin-10 (IL-10) is described as a cytokine that exerts immune downregulating actions. In this regard, our study indicates that IL-10 activity on human saphenous veins is coupled to nitric oxide (NO) release. We demonstrated this phenomenon by using in vitro real-time amperometric measurement of NO levels in explanted human saphenous veins after IL-10 exposure. IL-10-induced NO release can inhibit the adherence of monocytes (75.7 +/- 15 cells/600 microm2 of endothelial surface) and granulocytes (65 +/- 18 cells/600 microm2 of endothelial surface) from control values (250-300 cells/600 microm2 of endothelial surface; p < 0.005). This inhibition is directly sensitive to NO synthase inhibition. The specificity of the IL-10 effects is shown by its sensitivity to antibody. In vivo measurement of IL-10 levels during and after cardiopulmonary bypass surgery indicated that they are higher at 6 h after skin closure (1,400 pg/ml) compared with levels found during surgery (300 pg/ml). We surmise that the postsurgical increase of IL-10 levels may be an immunoregulatory attempt to downregulate the diffuse inflammation that has been shown to be associated with cardiopulmonary bypass surgery.
Stefano
G B
GB
Cardiac Research Program, University Medical Center at State University of New York at Stony-Brook, U.S.A.
Christensen
V B
VB
Tonnesen
E
E
Liu
Y
Y
Hughes
T K
TK
Bilfinger
T V
TV
D000368:Aged; D000906:Antibodies / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000276:immunology / Q000502:physiology*; D006098:Granulocytes / Q000276:immunology / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology*; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D012501:Saphenous Vein / Q000276:immunology / Q000378:metabolism / Q000502:physiology*
D000906:Antibodies; D016753:Interleukin-10; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase