Construction of a Lotus japonicus late nodulin expressed sequence tag library and identification of novel nodule-specific genes.
A range of novel expressed sequence tags (ESTs) associated with late developmental events during nodule organogenesis in the legume Lotus japonicus were identified using mRNA differential display; 110 differentially displayed polymerase chain reaction products were cloned and analyzed. Of 88 unique cDNAs obtained, 22 shared significant homology to DNA/protein sequences in the respective databases. This group comprises, among others, a nodule-specific homolog of protein phosphatase 2C, a peptide transporter protein, and a nodule-specific form of cytochrome P450. RNA gel-blot analysis of 16 differentially displayed ESTs confirmed their nodule-specific expression pattern. The kinetics of mRNA accumulation of the majority of the ESTs analyzed were found to resemble the expression pattern observed for the L. japonicus leghemoglobin gene. These results indicate that the newly isolated molecular markers correspond to genes induced during late developmental stages of L. japonicus nodule organogenesis and provide important, novel tools for the study of nodulation.
Szczyglowski
K
K
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing 48824, USA. szczyglw@pilot.msu.edu
Hamburger
D
D
Kapranov
P
P
de Bruijn
F J
FJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D007887:Fabaceae / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots; D010946:Plants, Medicinal; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D016324:Sequence Tagged Sites
D018076:DNA, Complementary; D008565:Membrane Proteins; D010940:Plant Proteins; C045625:nodulin