title: Presence of an unusually high concentration of an ubiquitinated histone-like protein in Trypanosoma cruzi.
abstract: The conjugation of ubiquitin to histones H2A and H2B has been established in higher eukaryotes and has been related to changes in chromatin organization. In Trypanosoma cruzi, no condensation of chromatin occurs during mitosis. In order to determine the presence of histone ubiquitination in T. cruzi epimastigotes, histones were extracted from chromatin and analyzed by three electrophoretic systems: acid-urea, triton-acid-urea and sodium-dodecyl-sulphate polyacrylamide gel. The immunochemical detection of ubiquitin-histone conjugates by Western blotting showed a strong reaction with a slow migrating band of M(r) 19 kDa. The high percentage of ubiquitin-histone conjugates present in T. cruzi chromatin may be related to the inability of this parasite to condense chromatin into a 30 nm fiber.
authors.affiliation: Department of Cell Biology, Universidad Simón Bolívar, Caracas, Venezuela.
authors.forename: L
authors.initials: L
authors.lastname: Reverol
authors.forename: M
authors.initials: M
authors.lastname: Chirinos
authors.forename: D A
authors.initials: DA
authors.lastname: Henriquez
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002457:Cell Extracts / Q000737:chemistry; D002843:Chromatin / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D007118:Immunoassay / Q000379:methods / Q000592:standards; D007120:Immunochemistry; D015800:Protozoan Proteins / Q000032:analysis / Q000276:immunology; D012995:Solubility; D014349:Trypanosoma cruzi / Q000737:chemistry*; D014452:Ubiquitins / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; D002843:Chromatin; D006657:Histones; D015800:Protozoan Proteins; D014452:Ubiquitins