Excellence of the two-layer method (University of Wisconsin solution/perfluorochemical) in pancreas preservation before islet isolation.
BACKGROUND
In islet transplantation pancreatic preservation before islet isolation is an obstacle compromising islet yield and viability. We tested the feasibility of a two-layer method (University of Wisconsin solution [UW]/perfluorochemical) for pancreatic preservation before islet isolation.


METHODS
Dog pancreases were processed into pure islets by the method of Ricordi preceded by five different preservations (groups 1-a and 1-b, the two-layer method for 3 and 24 hours; groups 2-a and 2-b, simple cold storage in UW for 3 and 24 hours; group 3, without preservation). Islet yields and functional success after autotransplantation into the liver were compared among the groups.


RESULTS
Postpurification islet equivalents (IE)/gm pancreas and functional success rate were 5600 (mean), 83% in group 1-a; 4000, 56% in group 1-b; 4700, 33% in group 2-a; 1300, 0% in group 2-b; and 5000, 89% in group 3 (p < 0.05; 2b versus 1-a, 1-b, and 3), respectively. There was no statistical difference among groups 1-a, 1-b, and 3 in terms of islet yield and function (p > 0.2).


CONCLUSIONS
The two-layer method is more effective than conventional simple cold storage in UW for pancreatic preservation before islet isolation. Clinical trials with the two-layer method are warranted.
Tanioka
Y
Y
Department of Surgery, University of Minnesota, Minneapolis, USA.
Sutherland
D E
DE
Kuroda
Y
Y
Gilmore
T R
TR
Asaheim
T C
TC
Kronson
J W
JW
Leone
J P
JP
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D004285:Dogs; D005260:Female; D005978:Glutathione; D007328:Insulin; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000502:physiology*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010179:Pancreas / Q000166:cytology; D010180:Pancreatectomy; D011887:Raffinose; D014182:Transplantation, Autologous; D016042:Transplantation, Heterotopic
D001786:Blood Glucose; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose