title: Mothers against decapentaplegic-related protein 2 expression in avian granulosa cells is up-regulated by transforming growth factor beta during ovarian follicular development.
abstract: Although mothers against dpp (MAD) and its related proteins (MADR) are believed to be important components of the cell signaling pathway for the transforming growth factor beta (TGFbeta) superfamily, the presence and regulation of these signaling molecules in ovarian cells by TGFbeta is not known. In the present studies, we have examined the presence of MADR2 and MADR1, two members of the MADR family, in hen granulosa cells at different stages of follicular development. The influence of TGFbeta in vitro on their expression was assessed, particularly in the context of TGFbeta-induced down-regulation of cytosolic phospholipase A2 (cPLA2), a key enzyme in the biosynthesis of eicosanoids. We have demonstrated for the first time the presence of MADR2 and MADR1 in hen granulosa cells at different stages of follicular development. The expression of MADR2, but not of MADR1, was up-regulated by TGFbeta in vitro in a concentration- and time-dependent manner. Granulosa cell MADR2 expression was maximal during early stages of follicular development, when the granulosa cell cPLA2 system is most responsive to the growth factor. The changes in MADR2 expression were accompanied by reciprocal alterations in the expression of cPLA2. These findings are consistent with the hypothesis that homologous up-regulation of MADR2 in granulosa cells may be an important determinant in its follicular stage-specific responsiveness to TGFbeta and possibly in the suppression of cPLA2 gene transcription by the growth factor.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Ottawa, Ottawa Civic Hospital Loeb Research Institute, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: P A
authors.initials: PA
authors.lastname: Hoodless
authors.forename: T
authors.initials: T
authors.lastname: Tzukazaki
authors.forename: J L
authors.initials: JL
authors.lastname: Wrana
authors.forename: L
authors.initials: L
authors.lastname: Attisano
authors.forename: B K
authors.initials: BK
authors.lastname: Tsang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000378:metabolism; D006080:Ovarian Follicle / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051785:Smad Proteins; D051899:Smad2 Protein; D015534:Trans-Activators; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D051785:Smad Proteins; D051899:Smad2 Protein; D015534:Trans-Activators; D016212:Transforming Growth Factor beta