An angiogenic protein from bovine serum and milk--purification and primary structure of angiogenin-2.
Bovine serum and milk contain a basic angiogenic protein that binds tightly to placental ribonuclease inhibitor. It was purified from both sources by ion-exchange and reversed-phase chromatographies. Its amino acid sequence revealed that it is a member of the ribonuclease superfamily. It contains 123 amino acids in a single polypeptide chain, is cross-linked by three disulfide bonds, is glycosylated at Asn33, and is 57% identical to bovine angiogenin. The amino-terminal and carboxyl-terminal residues are pyroglutamic acid and proline, respectively. The protein has ribonucleolytic activity that is similar to, but somewhat lower than, that of bovine angiogenin, i.e. very low relative to RNase. It is angiogenically potent on chicken chorioallantoic membrane, but less so than angiogenin. The sequence and activities demonstrate that this protein is a second, distinct, member of the angiogenin sub-family of pancreatic ribonucleases, and is referred to as angiogenin-2.
Strydom
D J
DJ
Center for Biochemical and Biophysical Sciences and Medicine, and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
Bond
M D
MD
Vallee
B L
BL
D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004220:Disulfides / Q000032:analysis; D005260:Female; D006801:Humans; D008892:Milk / Q000737:chemistry* / Q000201:enzymology; D008894:Milk Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010802:Phylogeny; D014176:Protein Biosynthesis / Q000187:drug effects; D011499:Protein Processing, Post-Translational; D012259:Ribonuclease, Pancreatic / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D043925:Angiogenesis Inducing Agents; D001798:Blood Proteins; D002241:Carbohydrates; D004220:Disulfides; D008894:Milk Proteins; D010446:Peptide Fragments; C046366:angiogenin; D012259:Ribonuclease, Pancreatic