Total ablation of cochlear haircells by perilymphatic perfusion with water.
Inner and outer haircells are destroyed within one day following perfusion of the perilymphatic spaces with water, otherwise the structural integrity of the cochlea and the organ of Corti are preserved. This technique is a reliable method to create an acute unilateral deafness model for investigations of auditory function.
Harrison
R V
RV
Department of Otolaryngology, The Hospital for Sick Children, Toronto, Ont., Canada.
Mount
R J
RJ
Hirakawa
H
H
D000818:Animals; D002682:Chinchilla; D003051:Cochlea / Q000473:pathology* / Q000648:ultrastructure; D003638:Deafness / Q000209:etiology; D004195:Disease Models, Animal; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010477:Perfusion; D010498:Perilymph / Q000187:drug effects; D014867:Water / Q000008:administration & dosage*
D014867:Water