Involvement of chloride channels in progesterone production during meiotic maturation of follicle-enclosed oocytes of Rana temporaria and Xenopus laevis.
The chloride channel blockers SITS (4-acetamido-4'-isothiocyanostibene-2,2'-disulphonic acid) and DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonic acid) markedly suppressed progesterone production in the Rana temporaria and Xenopus laevis follicle-enclosed oocytes and oocyte maturation stimulated by the homologous pituitary suspension and hCG, respectively. Inhibition was dose-dependent and decreased with the increase of the hormone concentration. SITS did not affect progesterone production in the R. temporaria follicle-enclosed oocytes stimulated by dbcAMP. Substitution of sodium chloride for equimolar concentrations of sodium gluconate, methanesulfonate, glutamate, or formate significantly potentiated the gonadotropin-stimulated progesterone production and oocyte maturation in the both species. Possible involvement of chloride channels in progesterone production by the gonadotropin-stimulated amphibian follicle-enclosed oocytes is discussed.
Skoblina
M N
MN
Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. SKOBLINA@IBRRAN.MSK.SU
Huhtaniemi
I
I
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000818:Animals; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism*; D002712:Chlorides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis*; D011896:Rana temporaria; D014982:Xenopus laevis
D018118:Chloride Channels; D002712:Chlorides; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D011374:Progesterone; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid