Beneficial effects of oxygenated fluorocarbon on the in vitro culture of protoplasts and cell electrofusion products.
Electrofused Passiflora protoplasts (P. edulis, P. giberti) were plated in KPR medium overlaying oxygen-gassed perfluorodecalin (Flutec PP6). Oxygenated PFC significantly (P < 0.05) enhanced protoplast division, as reflected by an increase in mean plating efficiency of up to 62% (P < 0.05) over 14 days. After 21 days of culture, the liquid phase containing dividing protoplast-derived cells, was removed from the PFC surface and overlaid onto MS-based agar medium for callus proliferation. Forty days later, protoplast-derived calli were transferred to one of two regeneration protocols, previously determined using unfused parental protoplasts. Calli derived from electrofusion-treated protoplasts exhibited organogenesis or somatic embryogenesis, depending on the regeneration procedure. The regeneration efficiency after 121 days for protoplasts initially cultured with oxygenated PFC was over 2-fold greater (P < 0.01) than control. These results indicate that oxygenated PFC can enhance growth and regeneration of protoplasts and their fusion products.
Wardrop
J
J
Department of Life Science, University of Nottingham, U.K.
Davey
M R
MR
Power
J B
JB
Lowe
K C
KC
D002459:Cell Fusion; D046508:Culture Techniques; D004558:Electric Stimulation; D005466:Fluorocarbons / Q000737:chemistry*; D006824:Hybridization, Genetic; D010100:Oxygen / Q000737:chemistry*; D059828:Plant Cells; D010944:Plants / Q000235:genetics*; D011523:Protoplasts; D012038:Regeneration / Q000502:physiology
D005466:Fluorocarbons; D010100:Oxygen