Conditional mutator phenotypes in hMSH2-deficient tumor cell lines.
Two human tumor cell lines that are deficient in the mismatch repair protein hMSH2 show little or no increase in mutation rate relative to that of a mismatch repair-proficient cell line when the cells are maintained in culture conditions allowing rapid growth. However, mutations accumulate at a high rate in these cells when they are maintained at high density. Thus the mutator phenotype of some mismatch repair-deficient cell lines is conditional and strongly depends on growth conditions. These observations have implications for tumor development because they suggest that mutations may accumulate in tumor cells when growth is limited.
Richards
B
B
Department of Oncological Sciences, Eccles Institute of Human Genetics, University of Utah, Salt Lake City, UT, 84112, USA.
Zhang
H
H
Phear
G
G
Meuth
M
M
D002452:Cell Count; D002455:Cell Division; D004260:DNA Repair; D004261:DNA Replication; D004268:DNA-Binding Proteins; D019008:Drug Resistance, Neoplasm; D005260:Female; D016368:Frameshift Mutation; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D018895:Microsatellite Repeats; D051718:MutS Homolog 2 Protein; D009154:Mutation; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D010042:Ouabain / Q000494:pharmacology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000235:genetics / Q000473:pathology
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D010042:Ouabain; D007041:Hypoxanthine Phosphoribosyltransferase; C497172:MSH2 protein, human; D051718:MutS Homolog 2 Protein