title: Integration of inulin determination in the AOAC method for measurement of total dietary fibre.
abstract: A suitable modification of the standard AOAC method for the measurement of dietary fibre is proposed to quantitatively include beta-fructans in the determination of the soluble dietary fibre fraction and as a consequence in the related total dietary fibre fraction. The standard AOAC method is modified by including a preheated commercial inulinase, Novozym SP 230, to the amyloglucosidase incubation step. It was previously outlined that this commercial inulinase contains some pectolytic activity. It is now demonstrated that a heat pretreatment of this enzyme preparation at 60 degrees C for 2 h eliminates this pectolytic activity while keeping sufficient activity to hydrolyse all the inulin from the soluble fibre fraction.
authors.affiliation: Laboratoire de Biochimie et Technologie des Glucides, Institut National de la Recherche Agronomique, Nantes, France. quemener@nantes.inra.fr
authors.forename: B
authors.initials: B
authors.lastname: Quemener
authors.forename: J F
authors.initials: JF
authors.lastname: Thibault
authors.forename: P
authors.initials: P
authors.lastname: Coussement
mesh_terms: D004043:Dietary Fiber / Q000032:analysis*; D004789:Enzyme Activation; D005504:Food Analysis / Q000379:methods*; D005630:Fructans / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism; D006358:Hot Temperature; D007444:Inulin / Q000032:analysis* / Q000378:metabolism; D012995:Solubility
chemical_list: D004043:Dietary Fiber; D005630:Fructans; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase