In vitro proliferative response of human peripheral blood mononuclear cells to concanavalin A.
The proliferation response of human peripheral blood mononuclear cells (PBMC) to concanavalin A (con A) was tested in a medium with or without addition of fetal calf serum (FCS) or a serum substitute. The time profile of the proliferative response of PBMC to con A was different in cells cultured in a medium supplemented with or without FCS. This different pattern occurred for the con A-induced DNA-synthesis as well as for the RNA- or protein-synthesis. The peak proliferation rate and the stimulation index of proliferation of human PBMC to con A was also higher when the cells were cultured in a medium with 10% FCS compared to cells cultured in a FCS-free medium. Substitution of the fetal calf serum by a serum substitute even induced a profound inhibition of the de novo synthesis of DNA in human PBMC. The results indicate that a lymphocyte proliferation response to con A can still be obtained in a culture medium where no FCS was added, although a weaker stimulation occurred in comparison to a culture medium with FCS. However, addition of a serum substitute caused a marked inhibition of the lymphocyte proliferation rate.
Lijnen
P
P
Department of Molecular and Cardiovascular Research, University of Leuven, Belgium.
Saavedra
A
A
Petrov
V
V
D001798:Blood Proteins / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis / Q000097:blood; D006801:Humans; D007930:Leucine / Q000097:blood; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis / Q000097:blood; D013936:Thymidine / Q000097:blood; D014316:Tritium; D014529:Uridine / Q000097:blood
D001798:Blood Proteins; D003470:Culture Media; D014316:Tritium; D003208:Concanavalin A; D012313:RNA; D004247:DNA; D007930:Leucine; D013936:Thymidine; D014529:Uridine