Evaluation of carbon-11-labelled phenoxy bridged fatty acids for studying myocardial fatty acid metabolism with PET.
A phenylene moiety in the chain of fatty acids was expected to impair metabolic degradation. Three phenoxy-containing [11C]carboxyl-labelled fatty acids were synthesized and evaluated in mice and an in vivo tissue distribution study. Of these three, 1[11C]-3-(p-dodecyloxyphenyl)propionic acid (C12C3) showed the most favourable uptake in the myocardium: 1.2% of the injected dose at 30 min p.i., vs. 0.6% for [11C]palmitate. The metabolic stability of C12C3 and [11C]palmitate was assessed by determining the amount of exhaled [11C]CO2 during a 30-min interval after injection. It was found that the phenoxy moiety in the gamma-position did not prevent the metabolic degradation of C12C3: After 30 min 20.7% of the injected dose was exhaled as [11C]CO2 vs. 12.7% for [11C]palmitate.
de Groot
T
T
Laboratory for Radiopharmaceutical Chemistry FFW, University Hospital Gasthuiseberg, Leuven, Belgium.
Maris
B
B
Boonen
C
C
Bormans
G
G
Mortelmans
L
L
Eisenhut
M
M
Verbruggen
A
A
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D005227:Fatty Acids / Q000378:metabolism*; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D014055:Tomography, Emission-Computed
D002250:Carbon Radioisotopes; D005227:Fatty Acids; D002245:Carbon Dioxide