[Activation of cGMP-dependent protein kinase from spleen lymphocytes of rats exposed to radiation].
We have emanated and partially refined cGMP-dependent protein kinase from cytosolic fraction of lymphocytes. 12 hours after X-irradiation of animals in the doses of 0.5 and 1 Gr. Optimal conditions for the occurrence of phosphotransferase reaction have been also fixed. Concentration of enzymes protein was 0.4 mg/ml, Mg(2+)-10 mM, pH-7.5-8.0 and incubation time 20 min. The purified enzyme was maximally active at 10(-7) M cGMP. Protein kinase activity had a dose-dependent character: the doses being increased the effect becomes stronger.
Ostapchenko
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D000818:Animals; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism*; D003600:Cytosol / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004789:Enzyme Activation; D005260:Female; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000528:radiation effects*
D017869:Cyclic GMP-Dependent Protein Kinases