title: Sex differences in biotransformation of the xenobiotic p-nitroanisole in isolated rat hepatocyte under the influence of phenobarbital.
abstract: Different sensitivity of the xenobiotic biotransformation system to phenobarbital treatment in female and male rat hepatocytes is shown. Hepatocytes isolated from females had less cytochrome P-450 content and metabolized the xenobiotic p-nitroanisole (p-NA) more slowly than hepatocytes from males. Phenobarbital treatment increased the cytochrome P-450 amount and the p-nitrophenol (p-NPh) formation rate in hepatocytes from female and male rats to the same extent. However, in control and female induced cells the main form of p-NPh was so conjugates, while induction of male rats reactions of conjugation were depressed and free p-NPh accumulated. The sex differences in the response of xenobiotic biotransformation system to an inducing treatment should be taken into account into transplantation and prescription of drug treatment courses.
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine, National Academy of Science of Ukraine, Kharkov, Ukraine. pay@pay.kharkov.ua
authors.forename: S P
authors.initials: SP
authors.lastname: Mazur
authors.lastname: Petrenko AYu
mesh_terms: D000818:Animals; D000840:Anisoles / Q000378:metabolism*; D001711:Biotransformation; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005260:Female; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009596:Nitrophenols / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D000840:Anisoles; D009596:Nitrophenols; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System; C005306:4-nitroanisole; C024836:4-nitrophenol; D010634:Phenobarbital