Preparation and characterization of monoclonal antibodies to foot-and-mouth disease virus type Asia-1.
Eight clones of hybrid cells secreting monoclonal antibodies to foot-and-mouth disease virus type Asia-1 were prepared; three of them neutralized viral infection. The specificity of THE monoclonal antibodies was analyzed by various immunoenzyme assays using 146S viral particles, trypsin-treated 146S particles, 12S particles, and certain viral polypeptides. The epitopes unique for virus type Asia-1 and conservative among several types were detected on the surface of viral particles. Epitopes on the surface of viral particle were mapped.
Amineva
S P
SP
Institute of Animal Health, Russian Ministry of Agriculture and Food, Vladimir Region, Russia.
Davydova
N L
NL
Mamatkulova
L U
LU
Globenko
L A
LA
Mishchenko
V A
VA
D000911:Antibodies, Monoclonal / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D002999:Clone Cells; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D005536:Foot-and-Mouth Disease / Q000276:immunology; D009500:Neutralization Tests; D014771:Virion / Q000276:immunology
D000911:Antibodies, Monoclonal