[Reconstitution of Hu-SCID mice transplanted with human tonsillar lymphocytes immunized with dengue virus-2 in vitro and its immune response].
OBJECTIVE
To study the measure for the reconstitution of severe combined immunodeficient disease mice (SCID mice) transplanted with human lymphocytes immunized with specific antigen in vitro.


METHODS
Hu-SCID mice were constructed by intraperitoneal (i.p.) transfer of human tonsillar lymphocytes (TLC) first immunized with dengue virus-2 (DV2) in vitro, then boostered with different doses of DV2 antigen in vivo.


RESULTS
Human immunoglobulin (Ig) and specific human anti-DV2 antibodies could be detected in sera of the hu-SCID mice by ELISA. Two weeks after transplantion of the in vitro-immunized hu-TLC, the highest human IgG concentration was 412.86 micrograms/ml, two or four weeks later specific human anti-DV2 IgM or IgG and the neutralizing antibody could found in these sera, respectively, the highest titer was 1:6400. The levels of human IgG and human anti-DV2 antibodies were related to the dose of DV2 antigen. The presence of human B cells (CD20+) and T/cells (CD3+, CD4+, CD8+) were shown in the sleen, lung, intestine and mesentery of the hu-SCID mice as determined by immunoperoxidase staining, and the cells were distributed correspondingly with the lymphocyte cells in proper human tissue. Human anti-DV2 IgG could also be detected in the spleen cell culture obtained from the SCID mice.


CONCLUSIONS
By co-immunized in vitro and in vivo the SCID mice deduced stronger immune response for specific antigen, and the reconstitution of the human lymphocytes could be established successfully.
Zuo
L
L
Department of Microbiology, Guiyang Medical College.
Guo
H
H
Zhang
C
C
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D014066:Palatine Tonsil / Q000166:cytology; D011897:Random Allocation; D016511:Severe Combined Immunodeficiency / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M