title: Contribution of chondroitin-dermatan sulfate-containing proteoglycans to the function of rat mesenteric arteries.
abstract: Proteoglycans are an important nonfibrous matrix component of the arterial wall. Direct evidence for their role in resistance-sized arteries is lacking, although they likely have an important role in coordinating and regulating vessel behavior, presumably via interactions of their glycosaminoglycan chains or core proteins with other matrix molecules and/or the smooth muscle cell surface. The purpose of this study was to determine whether the removal of specific glycosaminoglycan chains from proteoglycans in resistance-sized mesenteric arteries would change the mechanical properties of the arterial wall, thereby affecting their functional behavior. The major finding of the study was that 65% removal of chondroitin-dermatan sulfate-containing glycosaminoglycans from the arterial wall increased vascular wall stiffness and altered the myogenic behavior of the artery. The significant alterations in myogenic behavior associated with changes in passive mechanics following partial glycosaminoglycan chain removal support our hypothesis that chondroitin-dermatan sulfate-containing proteoglycans contribute significantly to the functional behavior of resistance arteries. We speculate that these alterations are the result of changes in stress transfer between collagen fibrils and/or stress transfer between cells and collagen fibrils under applied pressure.
authors.affiliation: Magee-Womens Research Institute, Pittsburgh, Pennsylvania, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Gandley
authors.forename: M K
authors.initials: MK
authors.lastname: McLaughlin
authors.forename: T J
authors.initials: TJ
authors.lastname: Koob
authors.forename: S A
authors.initials: SA
authors.lastname: Little
authors.forename: L J
authors.initials: LJ
authors.lastname: McGuffee
mesh_terms: D000818:Animals; D002807:Chondroitin / Q000502:physiology*; D003871:Dermatan Sulfate / Q000502:physiology*; D004548:Elasticity; D005260:Female; D066298:In Vitro Techniques; D008638:Mesenteric Arteries / Q000502:physiology*; D011509:Proteoglycans / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D014655:Vascular Resistance; D014661:Vasoconstriction; D014664:Vasodilation
chemical_list: D011509:Proteoglycans; D003871:Dermatan Sulfate; D002807:Chondroitin