Photoaffinity labeling and photoaffinity crosslinking of enzymes.
Photoaffinity labeling is a special type of chemical modification, where the label is activated by the action of light. This article presents the general principles and limitations of this technique, its application to the study of Micrococcus luteus ATPase and the use of photoaffinity crosslinking to probe the structure of this enzyme.
Schäfer
H J
HJ
Institut für Biochemie, Johannes-Gutenberg-Universität, Mainz, Germany.
Schuhen
A
A
D000244:Adenosine Diphosphate / Q000737:chemistry*; D000255:Adenosine Triphosphate / Q000737:chemistry*; D000345:Affinity Labels / Q000737:chemistry*; D004789:Enzyme Activation / Q000502:physiology*; D005609:Free Radicals / Q000737:chemistry*; D008024:Ligands; D016982:Micrococcus luteus / Q000201:enzymology*; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000648:ultrastructure
D000345:Affinity Labels; D005609:Free Radicals; D008024:Ligands; D010455:Peptides; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases