Endothelial cell expression of testican mRNA.
By screening random cDNAs from a continuous vascular endothelial cell line, EA.hy926, we identified a 5 kb mRNA that is expressed at high levels by this human cell line and by an early passage umbilical vein endothelial cell line. It is detected at lower levels in certain stromal cell lines, but it is not detected in most other cell lines tested, indicating that it represents a differentially expressed function rather than a ubiquitous or housekeeping function. This mRNA was readily detected in samples derived from most human organs as might be expected for a gene expressed in the vascular wall. Sequencing of the 5 kb mRNA reveals its identity with 3.5 kb of previously published testis-derived cDNA sequence called testican (Alliel et al., 1993). Differential expression of this gene by endothelial cells contributes a new perspective on the potential function of testican.
Marr
H S
HS
Pathology Department, University of North Carolina at Chapel Hill 27599-7525, USA.
Basalamah
M A
MA
Edgell
C J
CJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002461:Cell Line, Transformed; D018076:DNA, Complementary / Q000235:genetics; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011509:Proteoglycans / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology
D018076:DNA, Complementary; D011509:Proteoglycans; D012333:RNA, Messenger