High-speed electrophoretic separation of DNA fragments using a short capillary.
Capillary electrophoresis using a replaceable gel buffer was applied to the separation of DNA fragments. A short effective length capillary (1-2 cm) at low electric field allowed the separation of a 20-1000 bp ladder in 1 min. Although similar separation speed was achieved with a longer capillary at high field, the resolution of larger fragments was degraded. The short effective length capillaries were able to separate the wildtype and mutant PCR products of the TGF-beta1 gene in under 45 s.
Chan
K C
KC
Chemical Synthesis and Analysis Laboratory, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702, USA.
Muschik
G M
GM
Issaq
H J
HJ
D004247:DNA / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D016133:Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000235:genetics
D016212:Transforming Growth Factor beta; D004247:DNA