title: Prevention of Helicobacter pylori infection by lactobacilli in a gnotobiotic murine model.
abstract: BACKGROUND
  Helicobacter pylori is a bacterium which causes gastric inflammatory diseases. Oral inoculation of H pylori usually results in only a temporary colonisation without a successful infection in the stomach of conventional mice in which lactobacilli are the predominant indigenous bacteria.
  
  
  OBJECTIVE
  To determine whether lactobacilli exert an inhibitory effect on colonisation by H pylori in the stomach.
  
  
  METHODS
  The effects of H pylori on attachment to murine and human gastric epithelial cells and the H pylori mediated release of interleukin-8 (IL-8) by these cells were examined in vitro. Lactobacillus salivarius infected gnotobiotic BALB/c mice and control germ free mice were inoculated orally with H pylori to examine whether L salivarius can inhibit colonisation by H pylori.
  
  
  RESULTS
  L salivarius inhibited both the attachment and IL-8 release in vitro. H pylori could not colonise the stomach of L salivarius infected gnotobiotic BALB/c mice, but colonised in large numbers and subsequently caused active gastritis in germ free mice. In addition, L salivarius given after H pylori implantation could eliminate colonisation by H pylori.
  
  
  CONCLUSIONS
  These findings suggest the possibility of lactobacilli being used as probiotic agents against H pylori.
authors.affiliation: Department of Infectious Diseases, Tokai University, School of Medicine, Kanagawa, Japan.
authors.forename: A M
authors.initials: AM
authors.lastname: Kabir
authors.forename: Y
authors.initials: Y
authors.lastname: Aiba
authors.forename: A
authors.initials: A
authors.lastname: Takagi
authors.forename: S
authors.initials: S
authors.lastname: Kamiya
authors.forename: T
authors.initials: T
authors.lastname: Miwa
authors.forename: Y
authors.initials: Y
authors.lastname: Koga
mesh_terms: D000818:Animals; D000898:Antibiosis / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000378:metabolism / Q000382:microbiology; D005856:Germ-Free Life; D016481:Helicobacter Infections / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000502:physiology*; D016209:Interleukin-8 / Q000378:metabolism; D007778:Lactobacillus / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D016209:Interleukin-8