Peptides homologous to extracellular loop motifs of connexin 43 reversibly abolish rhythmic contractile activity in rabbit arteries.
1. Phenylephrine (10 microM) evoked rises in tension in isolated rings of endothelium-denuded rabbit superior mesenteric artery. These increases consisted of a tonic component with superimposed rhythmic activity, the frequency of which generally remained constant over time but whose amplitude exhibited cycle-to-cycle variability. 2. The amplitude, but not the frequency, of the rhythmic activity was affected by a series of short peptides possessing sequence homology with extracellular loops 1 and 2 of connexin 43 (Cx43). Oscillatory behaviour was abolished at concentrations of 100-300 microM (IC50 of 20-30 microM), without change in average tone. No synergy was evident between peptides corresponding to the extracellular loops, and cytoplasmic loop peptides were biologically inactive. 3. The putative gap junction inhibitor heptanol mimicked the action of the extracellular loop peptides and abolished rhythmic activity at concentrations of 100-300 microM without effects on frequency. However, in marked contrast to the peptides, heptanol completely inhibited the contraction evoked by phenylephrine (IC50, 283 +/- 28 microM). 4. The presence of mRNA encoding Cx32, Cx40 and Cx43 was detected in the rabbit superior mesenteric artery by reverse transcriptase-polymerase chain reaction. Western blot analysis showed that Cx43 was the major connexin in the endothelium-denuded vessel wall. 5. We conclude that intercellular communication between vascular smooth muscle cells via gap junctions is essential for synchronized rhythmic activity in isolated arterial tissue, whereas tonic force development appears to be independent of cell-cell coupling. The molecular specificity of the peptide probes employed in the study suggests that the smooth muscle relaxant effects of heptanol may be non-specific and unrelated to inhibition of gap junctional communication.
Chaytor
A T
AT
Department of Diagnostic Radiology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Evans
W H
WH
Griffith
T M
TM
D000438:Alcohols / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D018031:Connexin 43 / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D017630:Connexins / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000502:physiology; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology; D019850:Heptanol; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism; D008297:Male; D017538:Mesenteric Artery, Superior / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism; D009991:Oscillometry; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D013997:Time Factors; D014158:Transcription, Genetic; D000097002:Gap Junction beta-1 Protein; D000097003:Gap Junction alpha-5 Protein
D000438:Alcohols; D018031:Connexin 43; D017630:Connexins; D019850:Heptanol; D010446:Peptide Fragments; D010656:Phenylephrine; D012333:RNA, Messenger; D000097002:Gap Junction beta-1 Protein; D000097003:Gap Junction alpha-5 Protein