title: Expression and quantification of the iC3b-binding protein in different Candida albicans strains and their morphological stages.
abstract: Expression and quantification of the iC3b-binding protein in yeast, germ-tube, and mycelial forms of several Candida albicans strains were studied. Ten isolates were obtained from patients with recurrent vaginal candidosis. The germ-tubes generated at 37 degrees C and the mycelial forms of all strains grown at 30 degrees C, as well as most of the mycelial forms grown at 37 degrees C, were able to bind complement-coated sheep erythrocytes (EAiC3b). ELISA results revealed that a decrease in the binding of EAiC3b by the mycelial form of strains CBS 5982 and K10 correlated with a decrease of the expression of the iC3b-binding protein, detected by cross-reaction with the monoclonal antibody OKM1, recognising the alpha chain of human CR3. Expression of the iC3b-binding protein in other strains, binding EAiC3b, was higher in the mycelial form or very similar to that of germ-tubes. The dependence of the expression of the iC3b-binding protein on the morphological stages of individual C. albicans isolates suggests a possible association with the virulence of these strains.
authors.affiliation: Institute of Hygiene, Leopold-Franzens University Innsbruck, Austria. bujdakova@fns.uniba.sk
authors.forename: H
authors.initials: H
authors.lastname: Bujdákova
authors.forename: R
authors.initials: R
authors.lastname: Würzner
authors.forename: M
authors.initials: M
authors.lastname: Klobusický
authors.forename: M P
authors.initials: MP
authors.lastname: Dierich
mesh_terms: D002176:Candida albicans / Q000302:isolation & purification / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000378:metabolism*; D003179:Complement C3b / Q000378:metabolism*; D005656:Fungal Proteins; D006801:Humans; D017463:Receptors, Complement 3b / Q000096:biosynthesis / Q000378:metabolism*; D013696:Temperature
chemical_list: D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D017463:Receptors, Complement 3b; C097687:alphaINT1 protein, Candida albicans; D003179:Complement C3b