[The role of eicosanoids and progesterone in the ovulation of the oocytes in the common frog].
Prostaglandin F2 alpha (1-5 micrograms/ml) stimulated ovulation in vitro of Rana temporaria oocytes in the absence of pituitary suspension and potentiated the effects of progesterone. The inhibitor of cyclooxygenase indomethacin (0.01-10 micrograms/ml) decreased the rate of oocyte ovulation stimulated by the pituitary suspension. An increased pituitary suspension concentration decreased the inhibitory effect of indomethacin. Indomethacin did not affect oocyte ovulation stimulated by prostaglandin F2 alpha or progesterone. The inhibition of ovulation by the chloride channel blocker SITS (10 microM) is partly relieved by prostaglandin F2 alpha or progesterone but completely eliminated by their mixture.
Skoblina
M N
MN
Kondrat'eva
O T
OT
Nikiforova
G P
GP
Huhtaniemi
I
I
D000818:Animals; D046508:Culture Techniques; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015237:Dinoprost / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods; D010120:Oxytocics / Q000494:pharmacology*; D010902:Pituitary Gland; D011374:Progesterone / Q000494:pharmacology*; D011896:Rana temporaria; D013535:Suspensions
D016861:Cyclooxygenase Inhibitors; D010120:Oxytocics; D013535:Suspensions; D011374:Progesterone; D015237:Dinoprost; D007213:Indomethacin