Two regions in c-myb proto-oncogene product negatively regulating its DNA-binding activity.
The c-myb proto-oncogene product (c-Myb) is a transcriptional regulator that binds to the specific DNA sequence. Deletion of the negative regulatory domain (NRD) in the carboxyl-proximal region of c-Myb results in both increased trans-activating capacity and oncogenic activation. One possible mechanism to modulate c-Myb activity is a regulation of DNA-binding activity. However, it is not known whether any region in NRD affects the in vivo DNA-binding activity of c-Myb. Using the highly transfectable cell line 293T, we developed a system to precisely measure the DNA-binding activity of Myb expressed in mammalian cells. Using this system, two regions in NRD were shown to repress DNA-binding activity. These results suggest that DNA-binding activity of c-Myb is independently regulated by multiple mechanisms through these subdomains.
Tanaka
Y
Y
Laboratory of Molecular Genetics, Tsukuba Life Science Center, RIKEN, Ibaraki, Japan.
Nomura
T
T
Ishii
S
S
D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D016350:Leucine Zippers / Q000502:physiology; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism*; D020598:Proto-Oncogene Proteins c-myb; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D015534:Trans-Activators; D004247:DNA