Differential display: identifying genes involved in cardiomyocyte proliferation.
An estimated 15,000 different mRNA species are expressed in a typical mammalian cell. The differential expression of mRNAs in both a temporal and cell-specific manner determines the fate of the cell and creates the organism. Analysis of this differential gene expression has become a central aim of many laboratories attempting to understand the mechanisms underlying various biological processes. Currently, we are using a technique called differential display to analyze the differential expression of genes in cardiomyocytes. Differential display is a rapid and powerful technique that was introduced by Liang and Pardee in 1992. Since that time, it has been successfully applied by several groups, and it is quickly becoming a standard method for studying differential gene expression. Here, we present a detailed article discussing the differential display methodology and how we have utilized it to identify potential genes involved in cardiomyocyte proliferation. Furthermore, we have provided a list of materials and supplied examples of data obtained, in an effort to allow the reader to perform the technique with success in their own laboratory.
Cormier-Regard
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Louisiana State University Medical Center, Department of Biochemistry and Molecular Biology, New Orleans 70112, USA.
Egeland
D B
DB
Tannoch
V J
VJ
Claycomb
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WC
D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D018507:Gene Expression Regulation, Developmental; D005796:Genes; D006352:Heart Ventricles / Q000166:cytology / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017422:Sequence Analysis, DNA