title: A flagellar sheath protein of Helicobacter pylori is identical to HpaA, a putative N-acetylneuraminyllactose-binding hemagglutinin, but is not an adhesin for AGS cells.
abstract: The gene encoding a 29-kDa flagellar sheath protein was cloned and found to be similar to hpaA, reported to encode an N-acetylneuraminyllactose-binding fibrillar hemagglutinin (D. G. Evans, T. K. Karjalainen, D. J. Evans, Jr., D. Y. Graham, and C. H. Lee, J. Bacteriol. 175:674-683, 1993). The transcriptional start was mapped by primer extension from Helicobacter pylori mRNA, indicating an active consensus promoter at a location different from that suggested by Evans et al. Immunogold labelling of the flagellar sheath with a monoclonal antibody to HpaA was demonstrated in four strains, contrary to previous reports of a surface (D. G. Evans, T. K. Karjalainen, D. J. Evans, Jr., D. Y. Graham, and C. H. Lee, J. Bacteriol. 175:674-683, 1993) or a cytoplasmic (P. W. O'Toole, L. Janzon, P. Doig, J. Huang, M. Kostrzynska, and T. J. Trust, J. Bacteriol. 177:6049-6057, 1995) locale. Agglutination of erythrocytes and adherence to AGS cells by a delta hpaA mutant were no different from those of the parent strain, confirming a recent finding of O'Toole et al.
authors.affiliation: School of Biological Sciences, University of Birmingham, United Kingdom.
authors.forename: A C
authors.initials: AC
authors.lastname: Jones
authors.forename: R P
authors.initials: RP
authors.lastname: Logan
authors.forename: S
authors.initials: S
authors.lastname: Foynes
authors.forename: A
authors.initials: A
authors.lastname: Cockayne
authors.forename: B W
authors.initials: BW
authors.lastname: Wren
authors.forename: C W
authors.initials: CW
authors.lastname: Penn
mesh_terms: D018829:Adhesins, Bacterial; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004848:Epithelium / Q000382:microbiology; D004912:Erythrocytes / Q000382:microbiology; D005407:Flagella / Q000737:chemistry*; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000235:genetics; D016480:Helicobacter pylori / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D006388:Hemagglutinins / Q000032:analysis* / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013270:Stomach / Q000166:cytology / Q000382:microbiology; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D018829:Adhesins, Bacterial; D006388:Hemagglutinins; C095977:adhesin A, Helicobacter