Effective number of breeding adults in Bufo bufo estimated from age-specific variation at minisatellite loci.
Estimates of the effective number of breeding adults were derived for three semi-isolated populations of the common toad Bufo bufo based on temporal (i.e. adult-progeny) variance in allele frequency for three highly polymorphic minisatellite loci. Estimates of spatial variance in allele frequency among populations and of age-specific measures of genetic variability are also described. Each population was characterized by a low effective adult breeding number (Nb) based on a large age-specific variance in minisatellite allele frequency. Estimates of Nb (range 21-46 for population means across three loci) were approximately 55-230-fold lower than estimates of total adult census size. The implications of low effective breeding numbers for long-term maintenance of genetic variability and population viability are discussed relative to the species' reproductive ecology, current land-use practices, and present and historical habitat modification and loss. The utility of indirect measures of population parameters such as Nb and Ne based on time-series data of minisatellite allele frequencies is discussed relative to similar measures estimated from commonly used genetic markers such as protein allozymes.
Scribner
K T
KT
Department of Zoology, University of Leicester, UK. kim_scribner@nbs.gov
Arntzen
J W
JW
Burke
T
T
D000483:Alleles; D000818:Animals; D002023:Bufo bufo / Q000235:genetics; D004247:DNA / Q000302:isolation & purification; D004739:England; D005260:Female; D005618:Fresh Water; D005787:Gene Frequency; D008297:Male; D018598:Minisatellite Repeats; D011110:Polymorphism, Genetic; D011156:Population Density; D012098:Reproduction
D004247:DNA