title: [Optimization of the pertussis vaccine production process].
abstract: The production of Pertussis Vaccine was reevaluated at the Instituto Nacional de Higiene "Rafael Rangel" in order to optimise it in terms of vaccine yield, potency, specific toxicity and efficiency (cost per doses). Four different processes, using two culture media (Cohen-Wheeler and Fermentación Glutamato Prolina-1) and two types of bioreactors (25 L Fermentador Caracas and a 450 L industrial fermentor) were compared. Runs were started from freeze-dried strains (134 or 509) and continued until the obtention of the maximal yield. It was found that the combination Fermentación Glutamato Prolina-1/industrial fermentor, shortened the process to 40 hours while consistently yielding a vaccine of higher potency (7.91 +/- 2.56 IU/human dose) and lower specific toxicity in a mice bioassay. In addition, the physical aspect of the preparation was rather homogeneous and free of dark aggregates. Most importantly, the biomass yield more than doubled those of the Fermentador Caracas using the two different media and that in the industrial fermentor with the Cohen-Wheeler medium. Therefore, the cost per doses was substantially decreased.
authors.affiliation: División de Producción de Vacunas Bacterianas, Instituto Nacional de Higiene Rafael Rangel, Caracas, Venezuela.
authors.forename: J
authors.initials: J
authors.lastname: Germán Santiago
authors.forename: N
authors.initials: N
authors.lastname: Zamora
authors.forename: E
authors.initials: E
authors.lastname: de la Rosa
authors.forename: C
authors.initials: C
authors.lastname: Alba Carrión
authors.forename: P
authors.initials: P
authors.lastname: Padrón
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: M
authors.initials: M
authors.lastname: Betancourt
authors.forename: N
authors.initials: N
authors.lastname: Moretti
mesh_terms: D000818:Animals; D019149:Bioreactors; D001886:Bordetella pertussis / Q000254:growth & development; D003365:Costs and Cost Analysis; D005285:Fermentation; D051379:Mice; D010567:Pertussis Vaccine / Q000096:biosynthesis / Q000191:economics
chemical_list: D010567:Pertussis Vaccine