Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa.
In the Neurospora Genome Project at the University of New Mexico, expressed sequence tags (ESTs) corresponding to three stages of the life cycle of the filamentous fungus Neurospora crassa are being analyzed. The results of a pilot project to identify expressed genes and determine their patterns of expression are presented. 1,865 partial complementary DNA (cDNA) sequences for 1,409 clones were determined using single-pass sequencing. Contig analysis allowed the identification of 838 unique ESTs and 156 ESTs present in multiple cDNA clones. For about 34% of the sequences, highly or moderately significant matches to sequences (of known and unknown function) in the NCBI database were detected. Approximately 56% of the ESTs showed no similarity to previously identified genes. Among genes with assigned function, about 43.3% were involved in metabolism, 32.9% in protein synthesis and 8.4% in RNA synthesis. Fewer were involved in defense (6%), cell signalling (3.4%), cell structure (3.4%) and cell division (2.6%).
Nelson
M A
MA
Department of Biology, University of New Mexico, Albuquerque 87131, USA.
Kang
S
S
Braun
E L
EL
Crawford
M E
ME
Dolan
P L
PL
Leonard
P M
PM
Mitchell
J
J
Armijo
A M
AM
Bean
L
L
Blueyes
E
E
Cushing
T
T
Errett
A
A
Fleharty
M
M
Gorman
M
M
Judson
K
K
Miller
R
R
Ortega
J
J
Pavlova
I
I
Perea
J
J
Todisco
S
S
Trujillo
R
R
Valentine
J
J
Wells
A
A
Werner-Washburne
M
M
Natvig
D O
DO
D000595:Amino Acid Sequence; D001483:Base Sequence; D002455:Cell Division; D018076:DNA, Complementary / Q000737:chemistry; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D005800:Genes, Fungal; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis
D018076:DNA, Complementary; D005656:Fungal Proteins; D012333:RNA, Messenger