title: [Expression of IL-8 mRNA in glomerular endothelial cells].
abstract: OBJECTIVE
  To study the expression of IL-8 mRNA in cultured human glomerular endothelial cells.
  
  
  METHODS
  The effects of IL-1 beta and TNF alpha on the production of IL-8 by glomerular endothelial cells were also observed. RT-PCR was used.
  
  
  RESULTS
  There was only weak expression of IL-8 mRNA in cultured human glomerular endothelial cells without the presence of any stimulating factors, whereas the expression of IL-8 mRNA in cultured human glomerular endothelial cells was significantly increased after the glomerular endothelial cells have been treated with IL-1 beta (25 u/ml) or TNF alpha (10 ng/ml) for 24 hours.
  
  
  CONCLUSIONS
  Glomerular endothelial cell injury may enhance the expression of IL-8, which mediates the inflammatory reactions in glomeruli.
authors.affiliation: Department of Nephrology, PLA General Hospital, Beijing.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Liao
authors.forename: Y
authors.initials: Y
authors.lastname: Ye
mesh_terms: D002478:Cells, Cultured; D004727:Endothelium / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D053583:Interleukin-1beta; D016209:Interleukin-8 / Q000235:genetics*; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D053583:Interleukin-1beta; D016209:Interleukin-8; D010446:Peptide Fragments; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C052994:interleukin-1beta (163-171)