An efficient cost-effective protocol for automated fluorescent-DNA sequencing.
A simple and cost-effective method is described to sequence double-stranded DNA (< 100 ng) using a very small amount of fluorescent sequencing mix (one-tenth of manufacturer-recommended amount). The modification has significant advantages over conventional protocols and can be used to sequence, using a single sequencing kit, at least ten times the number of samples. This modified method also removes the post-reaction cleaning protocol and can be used for direct loading of samples on gels after completion of sequencing reactions. The accuracy of DNA sequencing data from analysis of 700 to 750 nucleotides is greater than 98.5%.
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Molecular Biology Core Facility, Division of Biomedical Sciences, Meharry Medical College, Nashville, TN 37208, USA.
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D001483:Base Sequence; D003362:Cost-Benefit Analysis; D004247:DNA / Q000235:genetics*; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000191:economics / Q000379:methods* / Q000592:standards
D005456:Fluorescent Dyes; D004247:DNA