Inactivation of hepatitis B virus: evaluation of the efficacy of the disinfectant 'Solprogel' using a DNA-polymerase activity assay.
The effects of sodium dichloroisocyanurate (NaDCC) and Solprogel (Laboratorios Inibsa, S.A., Barcelona, Spain), a compound that contains NaDCC plus a biodegradable polymer of acrylic acid, on the activity of DNA polymerase (DNA-P) associated with hepatitis B virus in serum were evaluated. DNA-P positive and negative pools of human serum samples were used as positive and negative stock virus. Inhibition of DNA-P activity by NaDCC and the commercial product was found to be concentration-dependent. Two minutes exposure to the minimum effective concentration of NaDCC (1000 ppm available chlorine) or Solprogel 16% (960 ppm available chlorine) totally inhibited DNA-P activity.
Hernández
A
A
Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Barcelona, Spain.
Belda
F J
FJ
Domínguez
J
J
Matas
L
L
Giménez
M
M
Caraballo
M
M
Ramil
C
C
Ausina
V
V
D000998:Antiviral Agents / Q000494:pharmacology*; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects; D004202:Disinfectants / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D005782:Gels; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D012996:Solutions; D014227:Triazines / Q000494:pharmacology*
D000998:Antiviral Agents; D004202:Disinfectants; D005782:Gels; D012996:Solutions; D014227:Triazines; D004259:DNA-Directed DNA Polymerase; C011765:troclosene