title: Burdock, a novel retrotransposon in Drosophila melanogaster, integrates into the coding region of the cut locus.
abstract: The burdock element is known to be the 2.6-kb insertion into the same region of the cut locus in 12 independently obtained ct-lethal mutants. Here we have determined the complete sequences of this insertion and of the hot spot region. It was found that the burdock is a short retrotransposon with long terminal repeats and a single open reading frame (ORF). The polypeptide encoded by the burdock ORF contains two adjacent regions homologous to the gag and pol polyproteins of the gypsy mobile element. The burdock insertion interrupts the short ORF of the cut locus. The target site sequence of the burdock insertions is similar to the Drosophila topoisomerase II cleavage site.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Science, Moscow.
authors.forename: N A
authors.initials: NA
authors.lastname: Ponomarenko
authors.forename: V M
authors.initials: VM
authors.lastname: Bannikov
authors.forename: V A
authors.initials: VA
authors.lastname: Anashchenko
authors.forename: N A
authors.initials: NA
authors.lastname: Tchurikov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D018398:Homeodomain Proteins; D007301:Insect Hormones / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements / Q000235:genetics*; D016415:Sequence Alignment; D014157:Transcription Factors
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D007301:Insect Hormones; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D018626:Retroelements; D014157:Transcription Factors; C066381:ct protein, Drosophila; D004250:DNA Topoisomerases, Type II