Short term storage of samples containing monoamines: ascorbic acid and glutathione give better protection against degradation than perchloric acid.
In order to study the protection of monoamines from degradation during short-time storage, the effect of three different antioxidants on the degradation of dopamine, dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIAA) was analyzed after 5 and 20 h. The results showed that dopamine was still quite stable after 20 h storage at room temperature, but that about 95% of 5-HIAA had disappeared. The best protection against degradation of all three substances was achieved when 15% v/v of a solution containing 1-2 mM ascorbic acid or 40 mM glutathione was added to the sample, resulting in near 100% protection after 20 h. Perchloric acid actually accelerated the degradation of 5-HIAA.
Boix
F
F
Department of Physiology, National Institute of Occupational Health, Oslo, Norway. fernando.boix@basalmed.uio.no
Wøien
G
G
Sagvolden
T
T
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D001205:Ascorbic Acid / Q000737:chemistry*; D015306:Biogenic Monoamines / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000032:analysis; D004356:Drug Storage; D005978:Glutathione / Q000737:chemistry*; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D010472:Perchlorates / Q000737:chemistry*
D015306:Biogenic Monoamines; D010472:Perchlorates; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D005978:Glutathione; D001205:Ascorbic Acid; D004298:Dopamine