Phylogenetic analysis of feline immunodeficiency virus isolated from cats in Taiwan.
Feline immunodeficiency virus was isolated from four cats in Taiwan. The isolates were designated TI-1, TI-2, TI-3 and TI-4. Each was isolated from PBMCs following co-cultivation of PBMCs with a feline T-lymphoblastoid cell line (MYA-1 cells). However, the Taiwanese isolates did not grow in a feline kidney cell line (CRFK cells). The nucleotide sequences of the V3-V5 region of the envelope gene of the Taiwanese isolates were determined and compared with those of previously described isolates. Phylogenetic analysis of this region indicates that Taiwanese isolates belong to subtype C.
Inada
G
G
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Miyazawa
T
T
Inoshima
Y
Y
Kohmoto
M
M
Ikeda
Y
Y
Liu
C H
CH
Lin
J A
JA
Kuo
T F
TF
Mikami
T
T
D000595:Amino Acid Sequence; D000818:Animals; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D002460:Cell Line; D018920:Coculture Techniques; D015752:Genes, env; D016089:Immunodeficiency Virus, Feline / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013624:Taiwan; D014779:Virus Replication