Phospholipase A2 is activated in the kidney, but not in the liver during ischemia-reperfusion.
Phospholipase A2 (PLA2) has been demonstrated to play an important role in the reperfusion injury of the kidney, gut, brain, heart and pancreas. This study was carried out to clarify whether PLA2 was involved in the ischemia-reperfusion injury of the liver. Rats were anesthetized and underwent laparotomy. They were allocated into one of 4 groups, i.e., the groups of renal ischemia (group RI), renal control (group RC), hepatic ischemia (group HI), and hepatic control (group HC). In group RI, the left renal pedicle was occluded for 1 hr, and the left kidney was removed after 1-hr reperfusion. In group HI, the portal and the hepatic artery supplying the left and middle lobes were clamped for 1 hr, followed by reperfusion. After predetermined periods of reperfusion up to 24 hr, the ischemic lobes were removed, homogenized and centrifuged. PLA2 activities in the mitochondrial fraction and the cytosolic fraction were measured with 14C-phosphatidylcholine (PC) and 14C-phosphatidylethanolamine (PE) as exogenous substrates. PLA2 activities of the both fractions in the kidney were significantly enhanced after 1-hr ischemia followed by 1-hr reperfusion. However, there was no enhancement of PLA2 activity of the either fraction in the group HI compared with the group HC. The results indicate that PLA2 is activated in the kidney but not in the liver during ischemia-reperfusion.
Terao
Y
Y
Intensive Care Unit, Nagasaki University School of Medicine, Japan.
Shibata
O
O
Goto
S
S
Morooka
H
H
Nakamura
H
H
Haseba
S
S
Sumikawa
K
K
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D004789:Enzyme Activation; D007511:Ischemia / Q000201:enzymology; D007668:Kidney / Q000098:blood supply / Q000201:enzymology*; D008099:Liver / Q000098:blood supply / Q000201:enzymology*; D008297:Male; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
D000410:Alanine Transaminase; D010741:Phospholipases A; D054467:Phospholipases A2