[Serodiagnosis of Helicobacter pylori infections by detection of immunoglobulin G antibodies with an immunoblot technique].
Serological testing has recently been proposed as an aid in diagnosis of H. pylori infections. In this study, an immunoblot method has been evaluated to diagnose H. pylori infection serologically by comparing 104 serum specimens from patients with a positive Gram stain and/or culture result and a positive urease test on biopsy material, as well as 30 serum specimens from patients with negative urease test, and negative microscopy and culture results. Thirteen bands could be identified by immunoblotting and were found significantly more frequent in patients with H. pylori infections than in patients without such infections. A semiquantitative grading system was introduced with score 1 for each reactive band and score 1/2 for a weakly reactive band. If score 2 was the cut-off value for IgG antibody, then the sensitivity, specificity, positive predictive value and negative predictive value of the immunoblot test were 100.0%, 90.0%, 97.2% and 100.0% respectively. There was no marked cross reaction with both Campylobacter jejuni and Campylobacter coli.
Pan
Z
Z
Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University.
Xiao
S
S
Jiang
S
S
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G