Somatostatin receptor subtype gene expression in human endocrine gastroentero-pancreatic tumours.
Somatostatin and its analogues are now of current use in the management of endocrine gastroentero-pancreatic (GEP) tumours for the purpose of inhibiting hormone hypersecretion, carrying scintigraphy imaging and attempting to slow down tumour growth. Recent molecular studies have revealed the existence of up to five membrane somatostatin receptor subtypes termed SSTR1-5. However, whether or not scintigraphy imaging and tumour characteristics are correlated with specific subtype(s) remains unclear. SSTR1-5 messenger RNA (mRNA) transcripts were investigated in 38 endocrine GEP tumours (32 islet cell tumours, six carcinoid) using reverse transcriptase polymerase chain reaction (RT-PCR), and their distribution was analysed with respect to tumour characteristics and scintigraphy imaging. SSTR2, SSTR5 and SSTR4 were detected in most cases of endocrine GEP tumours (92%, 84%, and 82% respectively), but SSTR1 and SSTR3 were less frequently observed (66% and 50% respectively). No clear-cut correlation was found between tumour characteristics and subtype mRNA distribution. Moreover, no differences in mRNA subtype distribution were found between the 17 tumours detected by scintigraphy and the four tumours not detected by this method. Somatostatin receptor mRNA subtypes are widely expressed in endocrine GEP tumours, but their distribution is not correlated with tumour characteristics or scintigraphy positivity.
Jaïs
P
P
Institut National de la Santé de la Recherche Médicale (Inserm) Unité 10, Department of Hepato-Gastroenterology, Pairs, France.
Terris
B
B
Ruszniewski
P
P
LeRomancer
M
M
Reyl-Desmars
F
F
Vissuzaine
C
C
Cadiot
G
G
Mignon
M
M
Lewin
M J
MJ
D007516:Adenoma, Islet Cell / Q000737:chemistry / Q000235:genetics*; D000328:Adult; D000368:Aged; D002276:Carcinoid Tumor / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D015408:Gastrinoma / Q000737:chemistry / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007340:Insulinoma / Q000737:chemistry / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D011877:Radionuclide Imaging; D017481:Receptors, Somatostatin / Q000235:genetics*; D013274:Stomach Neoplasms / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D012333:RNA, Messenger; D017481:Receptors, Somatostatin