title: Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli.
abstract: Recent advances in protein engineering have facilitated the development of alternative approaches to determine helix packing in polytopic membrane proteins. Using the lac permease as a paradigm, several site-directed biophysical and biochemical techniques are described which should be generally applicable.
authors.affiliation: Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024-1662, USA. RonaldK@HHMI.UCLA.edu
authors.forename: H R
authors.initials: HR
authors.lastname: Kaback
authors.forename: J
authors.initials: J
authors.lastname: Voss
authors.forename: J
authors.initials: J
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D008565:Membrane Proteins / Q000737:chemistry*; D026901:Membrane Transport Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D016297:Mutagenesis, Site-Directed; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D027981:Symporters
chemical_list: D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C021499:lactose permease