title: [Method of estimation of lysozyme activity in serum with chitin azure as substrate].
abstract: The method of estimation of lysozyme activity in serum when chitin azure is used as substrate is described. The basis of incubation medium is 0.1 M acetate buffer, pH 5.0. It was judged about activity of lysozyme by fluorescence of azure in incubation medium at the end of three hour incubation at 37 degrees C after separation on non-reacted chitin azure by short time centrifugation. It was shown that development of wound infection is accompanied by increasing of chitinase activity of lysozyme in serum.
authors.affiliation: Institute of Surgery of AV Vishnevskií RAMN, Moscow.
authors.forename: S A
authors.initials: SA
authors.lastname: Pisarzhevskií
authors.forename: A G
authors.initials: AG
authors.lastname: Globa
authors.forename: O N
authors.initials: ON
authors.lastname: Shirshov
authors.forename: A I
authors.initials: AI
authors.lastname: Marchuk
authors.forename: A M
authors.initials: AM
authors.lastname: Svetukhin
authors.forename: A A
authors.initials: AA
authors.lastname: Karelin
mesh_terms: D001399:Azure Stains; D015415:Biomarkers / Q000097:blood; D002686:Chitin; D006801:Humans; D009113:Muramidase / Q000097:blood*; D013050:Spectrometry, Fluorescence; D014946:Wound Infection / Q000201:enzymology
chemical_list: D001399:Azure Stains; D015415:Biomarkers; D002686:Chitin; D009113:Muramidase