The white gene from the yellow fever mosquito, Aedes aegypti.
We report the cloning and primary characterization of both cDNA and genomic fragments from the white gene of the yellow fever mosquito, Aedes aegypti. Comparisons of the conceptual translation product with white genes from four other species within the order Diptera show that the Ae. aegypti gene is most similar to the white gene of the mosquito vector of human malaria, Anopheles gambiae (86% identity and 92% similarity). The analysis of the primary sequence of genomic DNA at the 5'-end of the coding region revealed the presence of an intron that is also present in An. gambiae, but not in the vinegar fly, Drosophila melanogaster. The isolated clones of the Ae. aegypti white gene will enable the construction of a marker gene for use in the development of a germline transformation system for this species.
Coates
C J
CJ
Department of Molecular Biology and Biochemistry, University of California, Irvine 92697-3900, USA.
Schaub
T L
TL
Besansky
N J
NJ
Collins
F H
FH
James
A A
AA
D018528:ATP-Binding Cassette Transporters; D000330:Aedes / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics*; D005819:Genetic Markers; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic
D018528:ATP-Binding Cassette Transporters; D029721:Drosophila Proteins; D005136:Eye Proteins; D005819:Genetic Markers; C059254:w protein, Drosophila