Comparison of use of enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes for simultaneous detection of Entamoeba histolytica and E. dispar.
A comparison of the use of three commercially available enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes to detect and differentiate Entamoeba histolytica from E. dispar was carried out. Only the Techlab kit did not cross-react with E. dispar antigens, but it was 100 times less sensitive than PCR in detection of and differentiation between the two types of Entamoeba.
Mirelman
D
D
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel. bfmirelm@weizmann.weizmann.ac.il
Nuchamowitz
Y
Y
Stolarsky
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000913:Antibodies, Protozoan / Q000276:immunology / Q000302:isolation & purification; D000953:Antigens, Protozoan / Q000276:immunology / Q000302:isolation & purification; D003429:Cross Reactions / Q000276:immunology; D016054:DNA, Protozoan / Q000032:analysis; D003937:Diagnosis, Differential; D004747:Entamoeba / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004748:Entamoeba histolytica / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction / Q000379:methods*; D012335:RNA, Ribosomal / Q000235:genetics; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D012335:RNA, Ribosomal