Cyclic tensile stretch on bovine articular chondrocytes inhibits protein kinase C activity.
Osteoarthrosis, a common pathway of joint deterioration, is caused by mechanical stress loaded on articular cartilage. We previously demonstrated the involvement of protein kinase C (PKC) in the development of osteoarthritis in vitro. In this study, we examined the effect of mechanical stress on chondrocyte metabolism and the activity of PKC in vitro. Low frequency and magnitude of cyclic tensile stretch loaded on chondrocytes increased proteoglycan synthesis. However, high frequency and magnitude of stress decreased its synthesis. In this condition, activity of PKC was reduced. These results suggest an involvement of PKC in the stress-mediated inhibition of proteoglycan synthesis.
Fukuda
K
K
Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka, Japan.
Asada
S
S
Kumano
F
F
Saitoh
M
M
Otani
K
K
Tanaka
S
S
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D002417:Cattle; D048429:Cell Size; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D010003:Osteoarthritis / Q000209:etiology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011509:Proteoglycans / Q000096:biosynthesis*; D013314:Stress, Mechanical
D011509:Proteoglycans; D015240:Phorbol 12,13-Dibutyrate; D004247:DNA; D011493:Protein Kinase C