A sensitive PCR assay system for the quantitation of viral genome equivalents: human immunodeficiency virus type 1 (HIV-1) and hepatitis B virus (HBV).
A sensitive and reliable quantitative method based on the polymerase chain reaction (PCR) and the reverse transcription polymerase chain reaction (RT-PCR) to detect and quantify human immunodeficiency virus (HIV-1) and hepatitis B virus (HBV), respectively, was developed. The samples are co-processed together with two internal standards (calibrators). The amplicons are separated on denaturing polyacrylamide gels and co-detected and quantitated by laser induced fluorescence. HIV-1 and HBV containing biological samples, including samples from international test panels, were accurately quantitated. The procedure has proven to be a valuable tool in the quality control of biologicals such as plasma products and may serve to monitor disease progression and response to antiviral therapy.
Hämmerle
T
T
Immuno AG, Biomedical Research Center, Orth/Donau, Austria.
Himmelspach
M
M
Dorner
F
F
Falkner
F G
FG
D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D015750:Genes, gag; D016679:Genome, Viral; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014758:Viral Core Proteins / Q000235:genetics; D014766:Viremia
D017931:DNA Primers; D012367:RNA, Viral; D014758:Viral Core Proteins