The protein kinase C pseudosubstrate peptide (PKC19-36) inhibits insulin-stimulated protein kinase activity and insulin-mediated translocation of the glucose transporter glut 4 in streptolysin-O permeabilized adipocytes.
The effect of insulin on protein kinase activity and plasma membrane translocation of the glucose transporter GLUT 4 has been studied in adipocytes permeabilized by Streptolysin-O. Insulin increased protein kinase activity, and this was completely inhibited by the PKC pseudosubstrate inhibitor peptide (PKC19-36). Insulin-mediated translocation of GLUT 4 was also inhibited by the PKC inhibitor peptide. Both these insulin effects were blocked by a PKCbeta neutralizing antibody. Our results are consistent with the hypothesis that insulin activates PKCbeta activity in adipocytes in situ, and that this PKC activation is a component of the system whereby insulin regulates translocation of GLUT 4 to the plasma membrane.
Walaas
O
O
Neurochemical Laboratory, University of Oslo, Norway.
Horn
R S
RS
Walaas
S I
SI
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D001426:Bacterial Proteins; D015153:Blotting, Western; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D051275:Glucose Transporter Type 4; D007328:Insulin / Q000494:pharmacology*; D009004:Monosaccharide Transport Proteins / Q000187:drug effects / Q000502:physiology*; D009124:Muscle Proteins; D010446:Peptide Fragments / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000276:immunology / Q000494:pharmacology*; D064546:Protein Kinase C beta; D011494:Protein Kinases / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D013301:Streptolysins / Q000494:pharmacology
D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D010446:Peptide Fragments; C495694:Slc2a4 protein, rat; D013301:Streptolysins; C072093:protein kinase C (19-36); C025964:streptolysin O; D011494:Protein Kinases; D011493:Protein Kinase C; D064546:Protein Kinase C beta