Natural occurrence of fast- and fast/slow-muscle chimeric fibers in the expression of troponin T isoforms.
Rhomboideus, one of the back muscle tissues, and its single fibers were studied in chickens by immunostaining with antisera against fast- and slow-muscle-type troponin T isoforms. Nonuniform distribution of slow-muscle-type isoforms was for the first time detected in single fibers isolated from the muscle, although fast-muscle-type troponin T isoforms were distributed over the whole length of the fiber. Based on these observations, we conclude that fast- and fast/slow-muscle chimeric fibers exist in normal skeletal muscle tissue and that the existence of chimeric fibers is direct evidence showing that myonuclei subjected to different determination in troponin T isoform expression can together form a single muscle fiber.
Nakada
K
K
Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan.
Miyazaki
J I
JI
Saba
R
R
Hirabayashi
T
T
D000375:Aging; D000818:Animals; D002645:Chickens; D002678:Chimera; D007150:Immunohistochemistry; D018656:Muscle Fibers, Fast-Twitch / Q000166:cytology / Q000378:metabolism*; D018657:Muscle Fibers, Slow-Twitch / Q000166:cytology / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D014336:Troponin / Q000096:biosynthesis*; D020107:Troponin T
D014336:Troponin; D020107:Troponin T