Propofol assay in biological fluids in pregnant women.
A rapid, accurate and sensitive UV derivative method was described for measuring the Propofol concentration in some biological fluids. Furthermore two alternative procedures, a gaschromatographic and a colorimetric, were also defined, and the results of the three methods, when applied on blood samples spiked with known amounts of analyte, were compared. The samples were preliminary purified by a solid phase extraction on octadecyl C18 cartridge. The UV derivative method was applied to a pharmacokinetic study on pregnant women undergoing cesarean sections. After an induction dose administration of 2.5 mg kg-1, the maternal and umbilical vein blood were found to have comparable concentrations of propofol, with a mean half like of about 3.5 min; on the contrary no detectable levels of the drug were found in amniotic fluid. The drug recoveries > 98% and the response was linear over the range 0.05-40 micrograms ml-1.
Ragno
G
G
Pharmaco-Chemistry Department of University, Bari, Italy.
Cicinelli
E
E
Schonauer
S
S
Vetuschi
C
C
D000653:Amniotic Fluid / Q000737:chemistry; D018686:Anesthetics, Intravenous / Q000032:analysis*; D002849:Chromatography, Gas; D005260:Female; D006801:Humans; D011247:Pregnancy / Q000378:metabolism*; D015742:Propofol / Q000032:analysis*; D013053:Spectrophotometry
D018686:Anesthetics, Intravenous; D015742:Propofol