[Molecular weight determination and protein analysis of GCF-5 antigens of giant cell tumor of bone].
In order to understand the tumor antigen(s) of giant cell tumor of bone (GCT), monoclonal antibody GCF-5 developed in this laboratory was employed. GCF-5 reacted specifically against the tumor cell associated antigens of GCT. The membrane proteins of tumor cell were extracted by high concentration of SDS, followed by a series of treatment processes, including SDS-PAGE, western blotting and some staining methods, such as Periodic-Schiff for glycoprotein and oil red O for lipoprotein. The molecular weight and chemical characteristics of GCF-5 antigens were analysed. The preliminary results indicated that the main GCF-5 antigens were two glycoproteinis containing few saccharide chains, one of which was 13.5KD and the other 17.4KD.
Ren
X
X
Second Clinical Hospital, Beijing Medical University.
Zhou
L
L
Jiang
H
H
D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000302:isolation & purification; D001859:Bone Neoplasms / Q000276:immunology*; D018212:Giant Cell Tumor of Bone / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology; D008970:Molecular Weight
D000951:Antigens, Neoplasm; D008565:Membrane Proteins