title: Structure of the acid-labile galactosyl phosphate-containing O-antigen of the bacterium Proteus vulgaris OX19 (serogroup O1) used in the Weil-Felix test.
abstract: The structure of the O-specific polysaccharide chain of Proteus vulgaris OX19 lipopolysaccharide which determines the O1 specificity of Proteus and is used in the Weil-Felix test for diagnostics of rickettsiosis was established. On the basis of 1H- and 13 C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected 1H, 13C heteronuclear multiple-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY), it was found that the polysaccharide consists of branched pentasaccharide repeating units containing D-galactose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, and 2-acetamido-2,6-dideoxy-D-glucose (QuiNAc, two residues), which are connected to each other via a phosphate group (P): [formula: see text]. The polysaccharide is acid-labile, the glycosyl phosphate linkage being cleaved at pH 4.5 (70 degrees C) to give a phosphorylated pentasaccharide with a galactose residue at the reducing end. Structural analysis of the oligosaccharide and a product of its dephosphorylation with 48% hydrofluoric acid using 1H- and 13C-NMR spectroscopy and electrospray ionization mass spectrometry confirmed the structure of the polysaccharide.
authors.affiliation: Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: F V
authors.initials: FV
authors.lastname: Toukach
authors.forename: A
authors.initials: A
authors.lastname: Ziolkowski
authors.forename: A S
authors.initials: AS
authors.lastname: Swierzko
authors.forename: K I
authors.initials: KI
authors.lastname: Amano
authors.forename: W
authors.initials: W
authors.lastname: Kaca
authors.lastname: Knirel YuA
authors.forename: N K
authors.initials: NK
authors.lastname: Kochetkov
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011514:Proteus vulgaris / Q000737:chemistry*; D012373:Rocky Mountain Spotted Fever / Q000097:blood / Q000276:immunology; D013403:Sugar Phosphates / Q000737:chemistry*; D014438:Typhus, Epidemic Louse-Borne / Q000097:blood / Q000276:immunology
chemical_list: D019081:O Antigens; D013403:Sugar Phosphates