Micellar electrokinetic capillary chromatography with laser-induced fluorimetric detection of amines in beer.
Capillary electrophoresis with simultaneous ultraviolet absorbance and laser-induced fluorescence detection is applied to identify and quantify selected amines in beer following derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole. Quantitation was performed using the method of standard addition in order to avoid pH-dependent variations in the reactivity of the derivatizing agent with the added benefit of verifying peak identity. An inexpensive and "easy-to-use" on-column fiber optic fluorescence detection cell is described and implemented in the analysis.
Preston
L M
LM
Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore 21250, USA.
Weber
M L
ML
Murray
G M
GM
D000588:Amines / Q000032:analysis*; D001515:Beer / Q000032:analysis*; D019075:Electrophoresis, Capillary; D007834:Lasers; D008823:Micelles; D013050:Spectrometry, Fluorescence
D000588:Amines; D008823:Micelles