title: Influence of pH on phospholipid redistribution in human erythrocyte membrane.
abstract: The influence of the suspension pH (pHo) on the transmembrane mobility of spin-labeled phospholipid analogues in the human red blood cell was investigated. The passive transverse diffusion of spin-labeled phospholipid analogues was independent of pHo in the investigated range (5.8 to 8.5). However, upon acidification to pHo 5.8, a significant decrease of the rapid adenosine triphosphate (ATP)-dependent inward movement of aminophospholipids was found at physiologic ionic concentration, whereas a change of pH from 7.4 to 8.5 did not affect this transport. Evidence is given that the intracellular pH affects the active transport of aminophospholipids but not the extracellular pH. Suppression of the ATP-dependent outside-inside redistribution of aminophospholipid analogues by low pH was reversible because original transport activity was re-established upon reneutralization. pH dependence of the active phospholipid transport was not caused by the spin-labeled reporter group or by depletion of intracellular ATP. Because the same influence of pH on aminophospholipid movement could be observed for resealed ghosts, constituents of the red blood cell cytoplasm do not mediate the influence of pH on the ATP-dependent inward movement of aminophospholipids.
authors.affiliation: Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, Institut für Biologie/Biophysik, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Libera
authors.forename: T
authors.initials: T
authors.lastname: Pomorski
authors.forename: P
authors.initials: P
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Herrmann
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood; D017301:Ca(2+) Mg(2+)-ATPase / Q000097:blood; D002352:Carrier Proteins / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000097:blood; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000097:blood; D010718:Phosphatidylserines / Q000097:blood; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000097:blood*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013113:Spin Labels
chemical_list: D002352:Carrier Proteins; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010718:Phosphatidylserines; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D013113:Spin Labels; D012710:Serum Albumin, Bovine; D000255:Adenosine Triphosphate; D017301:Ca(2+) Mg(2+)-ATPase