[Studies on metabolism of 6-chloro butyl phthalide by rat liver microsomes].
The in vitro metabolism of 6-chloro-butyl phthalide (CBP) was studied by rat hepatic microsomes. The constitution of the incubation system was optimized and a RP-HPLC-DAD method was developed for the on-line analysis of CBP and its metabolites. The difference of the metabolism profiles of CBP by rat liver microsomes induced by phenobarbital (PB) and 3-methylcholanthrene (3-MC) was also compared. TLC, column chromatography and preparative HPLC were used for the isolation and purification of the major metabolites and they were identified as (R/S) gamma-hydroxy-CBP and (R/S) beta-hydroxy-CBP by UV, NMR and MS.
Peng
S H
SH
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Cui
Y Y
YY
Zhou
T H
TH
D000818:Animals; D000927:Anticonvulsants / Q000378:metabolism*; D001572:Benzofurans / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
C106182:6-chloro-butyl-phthalide; D000927:Anticonvulsants; D001572:Benzofurans