Mechanism of gluconate synthesis in Rhizobium meliloti by using in vivo NMR.
The dehydrogenation of [1-(13)C]- and [2-(13)C]glucose into gluconate was monitored by NMR spectroscopy in living cell suspensions of two Rhizobium meliloti strains. The synthesis of gluconate was accompanied, in the cellular environment, by the formation of two gluconolactones, a gamma-lactone being detected in addition to the expected delta-lactone. These lactones--as well as the gluconate--could be further metabolized by the cells. The delta-lactone was utilized faster than the gamma-lactone. The presence--in significant amounts--and the relative stability of the lactones raise the question of their possible physiological significance.
Portais
J C
JC
Laboratoire de Génie Cellulaire, UPRES-A CNRS 6022, Université de Picardie Jules Verne, Amiens, France.
Tavernier
P
P
Besson
I
I
Courtois
J
J
Courtois
B
B
Barbotin
J N
JN
D000468:Alkalies / Q000378:metabolism; D002247:Carbon Isotopes; D003470:Culture Media / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007783:Lactones / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D016962:Sinorhizobium meliloti / Q000254:growth & development* / Q000378:metabolism*
D000468:Alkalies; D002247:Carbon Isotopes; D003470:Culture Media; D005942:Gluconates; D007783:Lactones; D005947:Glucose