Inhibition of brain interleukin-1 attenuates sleep rebound after sleep deprivation in rabbits.
It is hypothesized that interleukin-1 (IL-1) is involved in physiological sleep. If this hypothesis is correct, inhibition of IL-1 should attenuate sleep responses after sleep deprivation. We tested the effect of intracerebroventricular or intravenous injection of an IL-1 inhibitor, an IL-1 receptor fragment (IL-1RF), on sleep rebound after sleep deprivation in rabbits. Six hours of total sleep deprivation significantly increased non-rapid eye movement sleep (NREMS) and enhanced electroencephalogram slow-wave activity during NREMS. Intracerebroventricular treatment with the IL-1RF (50 micrograms) significantly attenuated the sleep responses after sleep deprivation. Furthermore, 1.0 mg/kg i.v. injection of the IL-1RF significantly suppressed spontaneous NREMS in rabbits that were not sleep deprived. However, intravenous administration of the IL-1RF (1.0 mg/kg) failed to attenuate the sleep responses following the 6-h sleep deprivation period. These results support the hypothesis that central pools of IL-1 are important for physiological sleep regulation.
Takahashi
S
S
Department of Physiology and Biophysics, University of Tennessee, Memphis 38163, USA.
Fang
J
J
Kapás
L
L
Wang
Y
Y
Krueger
J M
JM
D000818:Animals; D001921:Brain / Q000378:metabolism*; D007275:Injections, Intravenous; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology; D011817:Rabbits; D017472:Receptors, Interleukin-1 / Q000737:chemistry; D012890:Sleep / Q000187:drug effects / Q000502:physiology*; D012892:Sleep Deprivation / Q000502:physiology*; D012894:Sleep Stages / Q000187:drug effects
D007375:Interleukin-1; D010446:Peptide Fragments; D017472:Receptors, Interleukin-1