Concentrations of lysosomal cysteine proteases are decreased in renal cell carcinoma compared with normal kidney.
Renal cell carcinoma contains significantly lower concentrations of the lysosomal cysteine proteases, cathepsins B, C, H, L and S, than does normal kidney, as shown by several methods, such as activity determination, enzyme-linked immunosorbent assay, immunoblotting and immunohistochemistry. The same low levels of enzyme activity and concentration have been determined in renal cell carcinoma metastases in the lung. Our results on the decreased concentration of cysteine peptidases at the protein level would seem to conflict with earlier results on an increased concentration of the cathepsin L mRNA in renal cell carcinoma.
Kirschke
H
H
Institute of Physiological Chemistry, Martin-Luther University, Halle (Saale), Germany.
Clausen
T
T
Göhring
B
B
Günther
D
D
Heucke
E
E
Laube
F
F
Löwe
E
E
Neef
H
H
Papesch
H
H
Peinze
S
S
Plehn
G
G
Rebmann
U
U
Rinne
A
A
Rüdrich
R
R
Weber
E
E
D002292:Carcinoma, Renal Cell / Q000201:enzymology*; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology*; D007680:Kidney Neoplasms / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D014407:Tumor Cells, Cultured
D004792:Enzyme Precursors; D002403:Cathepsins; C063905:procathepsin L; D056668:Cathepsin L