title: [A diagnostic analysis of a genetic mutation associated with a deficiency of leukocyte adhesion in cattle].
abstract: Bovine leukocyte adhesion (BLAD) is a recessive autosomal disease in Holstein-Friesian cattle caused by point mutation in CD18 gene encoding neutrophil-surface glycoprotein. To determine BLAD carriers, the convenient primers were chosen to amplify the mutant region of gene with the following restriction analysis. A screening program for BLAD has been initiated. Among 190 animals from different Ukrainian farms 6 were heterozygous according to the tested trait, i.e., BLAD deficient. No homozygous BLAD carriers were detected.
authors.forename: I E
authors.initials: IE
authors.lastname: Kostetskiĭ
authors.forename: S M
authors.initials: SM
authors.lastname: Kirilenko
authors.forename: V I
authors.initials: VI
authors.lastname: Glazko
authors.forename: A A
authors.initials: AA
authors.lastname: Sozinov
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D006580:Genetic Carrier Screening / Q000379:methods; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000235:genetics* / Q000662:veterinary*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D014455:Ukraine
chemical_list: D004247:DNA