Remyelination in the rat dorsal funiculus following demyelination by laser irradiation.
Excimer laser (KrF excimer laser, 248 nm wavelength) was used to damage cellular components in the dorsal funiculus at the lumbar level (L2) of the rat spinal cord. An open lesion was not found at the irradiation site on the spinal cord. However, the cytological examination revealed that cellular components were damaged to the depth of 200-500 microm from the pial surface. The characteristic feature was that at the border of the lesion, many axons remained naked but intact after their myelin sheaths had been completely disintegrated. Such naked axons were subsequently remyelinated by mature or immature glial cells. Mature oligodendrocytes, while retaining their cytoplasmic processes connected with the myelin sheaths of unaffected axons, extended new cytoplasmic processes on nearby naked axons and made new myelin sheaths around them. In contrast, 7 days after the irradiation, numerous immature glial cells appeared in association with naked axons, and some of them were differentiated into oligodendrocytes forming thin myelin sheaths on naked axons. These findings suggest that demyelinated axons can cause the proliferation and probably dedifferentiation of the oligodendrocyte lineage. The use of lasers provides a unique experimental model of demyelination and remyelination in the central nervous system of adult mammals.
Sato
K
K
Central Research Institute, Hamatatsu Photonics, Hamakita Research Park, Hamakita City, Shizuoka Prefecture, Japan.
Ohmae
E
E
Senoo
E
E
Mase
T
T
Tohyama
K
K
Fujimoto
E
E
Mizoguchi
A
A
Ide
C
C
D000818:Animals; D001369:Axons / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D002454:Cell Differentiation; D002455:Cell Division; D007834:Lasers; D008161:Lumbosacral Region; D008297:Male; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000502:physiology* / Q000528:radiation effects* / Q000648:ultrastructure; D009457:Neuroglia / Q000166:cytology / Q000502:physiology; D009836:Oligodendroglia / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000502:physiology* / Q000648:ultrastructure