High-frequency transposition of IS1373, the insertion sequence delimiting the amplifiable element AUD2 of Streptomyces lividans.
IS1373 is the putative insertion sequence delimiting the amplifiable unit AUD2 of Streptomyces lividans. Two IS1373-derived thiostrepton-resistant transposons, Tn5492 and Tn5494, transposed into multiple sites of the S. lividans chromosome at frequencies as high as 0.4 and 1%, respectively. Hence, IS1373 is a functional insertion sequence and its unique open reading frame, insA, encodes the transposase.
Volff
J N
JN
Institut für Industrielle Genetik, Universität Stuttgart, Germany. volff@biozentrum.uni-wverzburg.de
Altenbuchner
J
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004352:Drug Resistance, Microbial; D005822:Genetic Vectors / Q000235:genetics; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013883:Thiostrepton / Q000494:pharmacology; D014169:Transformation, Bacterial; D019895:Transposases
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; D019895:Transposases; D013883:Thiostrepton