Goldfish gamma-preprotachykinin mRNA encodes the neuropeptides substance P, carassin, and neurokinin A.
Two cDNAs, size 969 bp and 1146 bp respectively, encoding goldfish gamma-preprotachykinin (gamma-PPT) were identified. Both cDNAs contain the same 345 bp open reading frame. The deduced 114-amino acid gamma-PPT contains the sequence of substance P, carassin and neurokinin A. sequence analysis of the two cDNA 5'-untranslated regions shows that the two cDNAs may represent different PPT-A gene transcripts resulting from the alternative transcriptional start sites. Expression of gamma-PPT mRNA was detected in a wide range of brain areas from the olfactory bulbs to the posterior brain region, as well as in the intestine, testis and pituitary.
Lin
X W
XW
Department of Biological Sciences, University of Alberta, Edmonton, Canada.
Peter
R E
RE
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000737:chemistry; D005399:Fishes; D006054:Goldfish; D008297:Male; D008322:Mammals; D008969:Molecular Sequence Data; D015288:Neurokinin A / Q000235:genetics*; D010449:Peptide Mapping; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011898:Ranidae; D016415:Sequence Alignment; D013373:Substance P / Q000235:genetics*; D015320:Tachykinins / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C044206:preprotachykinin; C067794:carassin; D013373:Substance P; D015288:Neurokinin A