title: Genetic manipulation of anaerobic cellulolytic bacteria.
abstract: Transposon Tn1545 was introduced into the chromosome of the ruminal cellulolytic bacterium Eubacterium cellulosolvens. This was achieved by conjugal transfer of the transposon from Clostridium beijerinckii at a frequency of about 1 per 10(4) recipient cells. Transconjugants of E. cellulosolvens were resistant to both tetracycline and erythromycin. E. cellulosolvens could also serve as a donor for conjugal transfer of Tn1545 back into C. beijerinckii.
authors.affiliation: Department of Biological Sciences, Mississippi State University, MS 39762, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Anderson
mesh_terms: D000818:Animals; D001421:Bacteria, Anaerobic / Q000235:genetics*; D015139:Blotting, Southern; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000235:genetics; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D004917:Erythromycin; D005051:Eubacterium / Q000235:genetics*; D005818:Genetic Engineering; D007613:Kanamycin Resistance / Q000235:genetics; D012417:Rumen / Q000382:microbiology; D013753:Tetracycline Resistance / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004917:Erythromycin; D002482:Cellulose