Sex differences in biotransformation of the xenobiotic p-nitroanisole in isolated rat hepatocyte under the influence of phenobarbital.
Different sensitivity of the xenobiotic biotransformation system to phenobarbital treatment in female and male rat hepatocytes is shown. Hepatocytes isolated from females had less cytochrome P-450 content and metabolized the xenobiotic p-nitroanisole (p-NA) more slowly than hepatocytes from males. Phenobarbital treatment increased the cytochrome P-450 amount and the p-nitrophenol (p-NPh) formation rate in hepatocytes from female and male rats to the same extent. However, in control and female induced cells the main form of p-NPh was so conjugates, while induction of male rats reactions of conjugation were depressed and free p-NPh accumulated. The sex differences in the response of xenobiotic biotransformation system to an inducing treatment should be taken into account into transplantation and prescription of drug treatment courses.
Mazur
S P
SP
Institute for Problems of Cryobiology and Cryomedicine, National Academy of Science of Ukraine, Kharkov, Ukraine. pay@pay.kharkov.ua
Petrenko AYu
D000818:Animals; D000840:Anisoles / Q000378:metabolism*; D001711:Biotransformation; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005260:Female; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009596:Nitrophenols / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D015262:Xenobiotics / Q000378:metabolism
D000840:Anisoles; D009596:Nitrophenols; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System; C005306:4-nitroanisole; C024836:4-nitrophenol; D010634:Phenobarbital