Calmodulin-dependent cyclic nucleotide phosphodiesterase in adult and developing chick spinal cord.
We investigated the level and characteristics of "low Km" 3'-5' cyclic nucleotide phosphodiesterase (PDE) activity in adult and embryo chick spinal cord. The DEAE cellulose chromatography elution profile of Triton X-100 extracts showed a single peak of calmodulin-dependent cAMP/cGMP PDE activity. After two additional purification steps, this activity showed a five-fold activation by calmodulin (Ka = 1.5 nM) for cGMP hydrolysis, and a linear kinetic behaviour with a Km of 1.3 microM. Conversely, the activity showed a biphasic behaviour for cAMP hydrolysis, with Km values of 3.1 and 18.5 microM. The enzyme showed a Stokes radius of 4.5 nm. Western blot analysis of the purified enzyme revealed two immunoreactive bands with molecular mass of 59 and 65 kDa, respectively. Immunohistochemical staining showed motoneuron decoration both on cell soma and fibres. The developmental pattern of Ca2+-calmodulin-dependent PDE expression in spinal cord was also studied; the hydrolytic activity for both substrates has been found to increase constantly from E5 to post-hatching stages, when it appears 5.6-fold higher as compared to the early embryo levels. Furthermore, in cultured spinal cord neurons from E8 embryos, muscle extract has been shown to induce a two-fold increase of Ca2+-calmodulin-dependent cGMP activity. In conclusion, the studies reported here present three relevant findings: (1) the presence in adult and embryo chick spinal cord of PDE activities with characteristics similar to those of the mammalian PDE I enzyme; (2) its localization in the ventral horn motoneurons; (3) its regulated expression during embryogenesis that is possibly related to soluble epigenetic factors produced by the target cells.
Caniglia
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Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma La Sapienza, Rome, Italy.
Vignoli
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AL
Biagioni
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Augusti-Tocco
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Giorgi
M
M
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D015151:Immunoblotting; D007150:Immunohistochemistry; D007700:Kinetics; D016015:Logistic Models; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013116:Spinal Cord / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development
D010727:Phosphoric Diester Hydrolases; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1