Analysis of the role of the nnrR gene product in the response of Rhodobacter sphaeroides 2.4.1 to exogenous nitric oxide.
Rhodobacter sphaeroides 2.4.1, which is incapable of denitrification, has been found to carry nnrR, the nor operon, and nnrS, which are utilized for denitrification in R. sphaeroides 2.4.3. The gene encoding nitrite reductase was not found in 2.4.1. Expression of beta-galactosidase activity from a norB-lacZ fusion was activated when cells of 2.4.1 were incubated with NO-producing bacteria. This result indicates that the products of nnrR and the genes flanking it are utilized when 2.4.1 is growing in an environment where denitrification occurs.
Kwiatkowski
A V
AV
Department of Microbiology, Cornell University, Ithaca, New York 14853-8101, USA.
Laratta
W P
WP
Toffanin
A
A
Shapleigh
J P
JP
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*; D009572:Nitrite Reductases / Q000235:genetics; D009573:Nitrites / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics; D011993:Recombinant Fusion Proteins; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics*
D001426:Bacterial Proteins; D009573:Nitrites; C100897:NnrR protein, Rhodobacter sphaeroides; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D009569:Nitric Oxide; D010088:Oxidoreductases; D009572:Nitrite Reductases; C034747:nitric-oxide reductase; D009609:Nitrous Oxide