Beneficial effect of medium with high concentration serum for direct sperm injection into mouse oocytes using a conventional pipette.
Although the piezo-electrically driven pipette is much more effective than a conventional pipette for direct sperm injection into mouse oocytes, the piezo-pipette driving unit is expensive and may not be readily accessible to most basic science laboratories. We report here that the inefficiency of the conventional pipette can be improved by performing the operation in a medium with high serum concentration.
Suzuki
K
K
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822, USA.
Yanagimachi
R
R
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003433:Crosses, Genetic; D003470:Culture Media / Q000494:pharmacology*; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology; D005312:Fetal Blood / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008845:Microinjections / Q000295:instrumentation*; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*
D003470:Culture Media