Transcription termination factor Rho is essential for Micrococcus luteus.
The growth of Micrococcus luteus, a soil microorganism that belongs to the high-G+C gram-positive phylogenetic group, is prevented by bicyclomycin, an antibiotic that inhibits the activity of the M. luteus transcription termination factor Rho. A mutant that can grow in 0.3 mM bicyclomycin has a Rho that is insensitive to bicyclomycin and has the single amino acid residue change of Asp474 to Gly. These results indicate that the function of its Rho factor is essential for M. luteus and that growth of a gram-positive organism can be blocked by bicyclomycin.
Nowatzke
W L
WL
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Keller
E
E
Koch
G
G
Richardson
J P
JP
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001482:Base Composition; D001483:Base Sequence; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D003001:Cloning, Molecular; D016982:Micrococcus luteus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D008969:Molecular Sequence Data; D009154:Mutation; D012234:Rho Factor / Q000235:genetics / Q000502:physiology*
D000900:Anti-Bacterial Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D012234:Rho Factor; C024504:bicozamycin