title: Regulation of growth of human gastric cancer by gastrin and glycine-extended progastrin.
abstract: OBJECTIVE
  Gastrin (G-17) stimulates the growth of certain gastric and colon cancers mostly through gastrin/cholecystokinin (CCK)-B receptors. Glycine-extended gastrin (Gly-G) stimulates growth of a rat pancreatic acinar cell line; however, the effect of Gly-G on human gastric cancers is not known. The purpose of this study was to characterize the trophic effect of G-17 and Gly-G on two human gastric cancer cell lines, AGS and SIIA.
  
  
  METHODS
  Binding analyses were performed, and cell growth was assessed by counting cells over a time course.
  
  
  RESULTS
  G-17 stimulated growth of both AGS and SIIA cells. In AGS cells, gastrin/CCK-B receptor antagonists inhibited the effect of G-17 and competitively antagonized 125I-G-17 binding, whereas the CCK-preferring (CCK-A) receptor antagonists had no effect. In contrast, CCK-A receptor antagonists inhibited the stimulatory effect of G-17 in SIIA cells, whereas CCK-B receptor antagonists had no effect. Gly-G stimulated the growth of AGS and SIIA cells; neither the CCK-B nor the CCK-A receptor antagonists blocked this effect.
  
  
  CONCLUSIONS
  G-17 stimulates proliferation of AGS cells through the CCK-B receptor; however, G-17-mediated growth of SIIA acts through a CCK-A-like receptor. Furthermore, Gly-G stimulates growth of human gastric cancer cell lines, possibly through a receptor other than the CCK-B or CCK-A receptor.
authors.affiliation: Department of Surgery, University of Texas Medical Branch, Galveston, USA.
authors.forename: K
authors.initials: K
authors.lastname: Iwase
authors.forename: B M
authors.initials: BM
authors.lastname: Evers
authors.forename: M R
authors.initials: MR
authors.lastname: Hellmich
authors.forename: Y S
authors.initials: YS
authors.lastname: Guo
authors.forename: S
authors.initials: S
authors.lastname: Higashide
authors.forename: H J
authors.initials: HJ
authors.lastname: Kim
authors.forename: C M
authors.initials: CM
authors.lastname: Townsend
mesh_terms: D002455:Cell Division / Q000187:drug effects; D005755:Gastrins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D011498:Protein Precursors / Q000378:metabolism / Q000494:pharmacology*; D043886:Receptor, Cholecystokinin A; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin / Q000502:physiology; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D005755:Gastrins; D011498:Protein Precursors; D043886:Receptor, Cholecystokinin A; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin; C086854:glycine-extended gastrin 17; C013706:big gastrin