title: In situ hybridization study on tissue inhibitors of metalloproteinases (TIMPs) mRNA-expressing cells in human inflamed gingival tissue.
abstract: This study presents the exact cell types and localization of tissue inhibitors of metalloproteinases (TIMPs) production sites in periodontal diseased gingiva by means of in situ hybridization. Gingival tissue specimens were fixed, embedded and hybridized in situ with specific digoxigenin-labeled cRNA probes (386 and 496 bp). TIMP-1 and -2 mRNAs were expressed on macrophages, mononuclear cells, capillary endothelial cells and some fibroblasts throughout the gingival tissue. In periodontitis, TIMP-1 and -2 mRNA-expressing cells showed significantly different localization. TIMP-1 mRNA was broadly observed in the gingival connective tissue while TIMP-2 mRNA was predominantly expressed in the connective tissue adjacent to the pocket epithelium (p < 0.01). Fewer TIMPs mRNA were observed in minimal gingivitis than in periodontitis, especially in the middle zone of gingival tissue. Thus, TIMP-1 and TIMP-2 mRNA was detected differentially and site-specifically in periodontal diseased gingival tissue.
authors.affiliation: Department of Periodontology, Niigata University School of Dentistry, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuki
authors.forename: T
authors.initials: T
authors.lastname: Nomura
authors.forename: K
authors.initials: K
authors.lastname: Hara
mesh_terms: D000368:Aged; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D003238:Connective Tissue / Q000201:enzymology / Q000473:pathology; D004076:Digoxigenin; D004730:Endothelium, Vascular / Q000201:enzymology / Q000473:pathology; D004848:Epithelium / Q000201:enzymology / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000473:pathology; D005881:Gingiva / Q000201:enzymology* / Q000473:pathology; D005891:Gingivitis / Q000201:enzymology* / Q000473:pathology; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000473:pathology; D008222:Lymphokines / Q000032:analysis / Q000235:genetics; D008264:Macrophages / Q000201:enzymology / Q000473:pathology; D008297:Male; D061965:Matrix Metalloproteinase Inhibitors; D008875:Middle Aged; D010514:Periodontal Pocket / Q000201:enzymology / Q000473:pathology; D010518:Periodontitis / Q000201:enzymology / Q000473:pathology; D011480:Protease Inhibitors / Q000032:analysis*; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D015347:RNA Probes; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D016610:Tissue Embedding; D016707:Tissue Fixation; D019716:Tissue Inhibitor of Metalloproteinase-2; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D002352:Carrier Proteins; D006023:Glycoproteins; D008222:Lymphokines; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors; D011506:Proteins; D015347:RNA Probes; D018075:RNA, Complementary; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D019716:Tissue Inhibitor of Metalloproteinase-2; D004076:Digoxigenin