Characterization of 5'-proximal sequence of mouse GABA transporter gene (GAT-1).
The cDNA molecule encoding the mouse GABA transporter gene (GAT-1) was used as probe for selecting GAT-1 gene from mouse genomic library. A positive clone, harboring the whole open reading frame of the GAT-1 protein and designated as MGABAT-G, was fished out from the library, the 5' proximal region and intron 1 were sequenced and analysed, and low homology was found in the above region between GAT-1 genes from mouse and human except some short conserved sequences. The DNA-protein interactions between DNA fragments containing the conserved sequences in the 5' proximal region and nuclear proteins from different tissues of mouse were studied by means of gel-shift assay, and Southern-Western blot. The results indicate a possible positive-negative regulation mode controlling the expression of the mouse GAT-1 gene.
Fei
J
J
Shanghai Institute of Cell Biology, Chinese Academy of Sciences.
Huang
F
F
Ma
Y H
YH
Guo
L H
LH
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002457:Cell Extracts; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D050485:GABA Plasma Membrane Transport Proteins; D005786:Gene Expression Regulation / Q000235:genetics; D005796:Genes / Q000235:genetics; D007438:Introns / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism; D009928:Organ Specificity; D027361:Organic Anion Transporters; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000235:genetics; D005680:gamma-Aminobutyric Acid
D002352:Carrier Proteins; D002457:Cell Extracts; D018076:DNA, Complementary; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009687:Nuclear Proteins; D027361:Organic Anion Transporters; C493413:SLC6A1 protein, human; C493414:Slc6a1 protein, mouse; D005680:gamma-Aminobutyric Acid; D004247:DNA