Characterization of serotype II polysaccharide antigen of group E streptococci using a monoclonal antibody.
A Monoclonal antibody (MAb II-T) specific for serotypes II and V Group E streptococci (GES) was prepared by fusing myeloma cells with spleen cells of mice immunized with whole cells of a serotype II strain. MAb II-T reacted in an enzyme immunoassay (EIA) with whole cells of both serotypes and reacted in gel diffusion test with autoclaved-saline extraction of serotypes II and V. The extract was purified by DEAE-Sephadex A-25, followed by treatment with proteinase K, and further by chromatography with a Sephadex G-200 column. The purified polysaccharide (PS) antigen contained 98.6% carbohydrate and 1.4% protein, but no detectable phosphorus. In hapten inhibition tests using various sugars, D-mannosamine markedly inhibited the precipitin reaction. These results indicated that the antigenic determinant might have a structure similar to D-mannosamine.
Tomotake
H
H
Department of Food Microbiology, School of Medicine, University of Tokushima, Japan.
Yamato
M
M
Hirota
K
K
Ota
F
F
D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003429:Cross Reactions; D006241:Haptens / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology*; D011233:Precipitin Tests; D012703:Serotyping; D013045:Species Specificity; D013291:Streptococcus / Q000145:classification* / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D006241:Haptens; D011135:Polysaccharides, Bacterial