RAP1-like binding activity in islet cells corresponds to members of the Sp1 family of transcription factors.
Deletion and mutational analyses of the gastrin promoter have identified a binding site for the yeast transcription factor RAP1 relevant for transcriptional activation in islet cells. We here report that the mammalian transcription factors binding to this site in islet cells are the Sp transcription factor members Sp1 and Sp3. Furthermore, functional analyses revealed Sp1- and Sp3-mediated transcriptional activation of gastrin. These data reveal that the zinc finger proteins Sp1 and Sp3 do have similar binding specificities as the multifunctional yeast RAP1 protein.
Simon
B
B
Department of Internal Medicine, Philipps-University, Marburg, Germany. simonb@post.med.uni-marburg.de
Wattler
F
F
Merchant
J L
JL
Münch
K
K
Schütze
H J
HJ
Suske
G
G
Arnold
R
R
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005755:Gastrins / Q000235:genetics*; D006801:Humans; D007515:Islets of Langerhans / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D017384:Sequence Deletion; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D051705:Sp3 Transcription Factor; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation; D014162:Transfection; D014407:Tumor Cells, Cultured; D020668:rap GTP-Binding Proteins
D004268:DNA-Binding Proteins; D005755:Gastrins; C497121:SP3 protein, human; D016329:Sp1 Transcription Factor; C497123:Sp3 protein, rat; D014157:Transcription Factors; D051705:Sp3 Transcription Factor; D003850:Deoxyribonuclease I; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins