Cloning of cDNA for goldfish activin beta B subunit, and the expression of its mRNA in gonadal and non-gonadal tissues.
We have cloned a full length cDNA coding for activin beta B subunit from the goldfish ovary. Sequence analysis of the goldfish activin beta B shows that this peptide is extremely conserved across vertebrates. The mature region of goldfish activin beta B has 93 and 98% amino acid identity with that of human and zebrafish beta B subunit respectively. The identity of the cloned goldfish activin beta B was further confirmed by expressing the protein in the Chinese hamster ovary (CHO) cells followed by detection of the specific activity of activin in the culture medium using F5-5 cell assay. mRNA of goldfish activin beta B is expressed in a variety of goldfish tissues including ovary, testis, brain, pituitary, kidney and liver, suggesting a wide range of physiological roles for activin in the goldfish.
Ge
W
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Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.
Miura
T
T
Kobayashi
H
H
Peter
R E
RE
Nagahama
Y
Y
D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D006054:Goldfish; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides; D010455:Peptides / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D009842:Oligopeptides; D010455:Peptides; D012333:RNA, Messenger; C044147:activin B; D028341:Activins; C048617:myelopeptides