title: Glass peek composite promotes proliferation and osteocalcin production of human osteoblastic cells.
abstract: An isoelastic intramedullary implant has been developed using a composite of polyetheretherketone and 10% random, chopped E-glass fibers (GPEEK). The effect of this novel material on human bone cells has not been defined. The objective of this study was to test whether GPEEK supported the proliferation of the human bone cell line MG63, which exhibits osteoblastlike characteristics. Cells (1 x 10(5)/mL) were propagated on GPEEK discs with three different surface roughnesses (3, 6, and 9 microns) and on polystyrene plates, for comparison. The reaction of MG63 osteoblastlike cells to the GPEEK polymer composite was analyzed by determination of cell yield, osteocalcin production, and levels of alkaline phosphatase. The viable cells that were retrieved from the GPEEK discs of all three surface roughness had an approximate sixfold increase in number. Osteoblastic function of the cells, indicated by osteocalcin production, was unimpaired after a 5-day culture on the three surfaces of GPEEK. The highest level of osteocalcin was produced by osteoblastic cells propagated on GPEEK with a 9 microns surface roughness. The levels of alkaline phosphatase of these cells were similarly greater for the different degrees of surface roughness. Overall, this study demonstrates that GPEEK supported proliferation of osteoblastlike cells and provided a favorable environment for the continued production of osteocalcin in vitro.
authors.affiliation: Department of Orthopedic Surgery, Good Samaritan Hospital, Johns Hopkins University, Baltimore, MD 21239, USA.
authors.forename: T W
authors.initials: TW
authors.lastname: Lin
authors.forename: A A
authors.initials: AA
authors.lastname: Corvelli
authors.forename: C G
authors.initials: CG
authors.lastname: Frondoza
authors.forename: J C
authors.initials: JC
authors.lastname: Roberts
authors.forename: D S
authors.initials: DS
authors.lastname: Hungerford
mesh_terms: D001577:Benzophenones; D001672:Biocompatible Materials; D001705:Bioprosthesis; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005898:Glass; D006801:Humans; D007659:Ketones; D010006:Osteoblasts / Q000166:cytology*; D011092:Polyethylene Glycols; D011108:Polymers
chemical_list: D001577:Benzophenones; D001672:Biocompatible Materials; D007659:Ketones; D011108:Polymers; C009753:fiberglass; C063834:polyetheretherketone; D011092:Polyethylene Glycols