An lrp-like gene of Bacillus subtilis involved in branched-chain amino acid transport.
The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transport. The product of the azlB gene is an Lrp-like protein that negatively regulates expression of the azlBCDEF operon. Resistance to 4-azaleucine in azlB mutants is due to overproduction of AzlC and AzlD, two novel hydrophobic proteins.
Belitsky
B R
BR
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Gustafsson
M C
MC
Sonenshein
A L
AL
Von Wachenfeldt
C
C
D000596:Amino Acids / Q000032:analysis; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D005970:Glutamate Synthase / Q000378:metabolism; D007930:Leucine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D051756:Leucine-Responsive Regulatory Protein; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics; D010802:Phylogeny; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014157:Transcription Factors
D000596:Amino Acids; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D051756:Leucine-Responsive Regulatory Protein; C000111:azaleucine; D005970:Glutamate Synthase; D007930:Leucine