title: Protein engineering as a powerful tool for the chemical modification of enzymes.
abstract: This article discusses the techniques of site-specific mutagenesis and protein engineering and their application in the study of enzyme active sites and the mechanism of enzyme action. Particular emphasis is given to beta-lactamase.
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
authors.forename: G
authors.initials: G
authors.lastname: Moreno-Hagelsieb
authors.forename: X
authors.initials: X
authors.lastname: Soberon
mesh_terms: D004789:Enzyme Activation; D004798:Enzymes / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D004798:Enzymes