A recombinant human fibronectin fragment facilitates retroviral mediated gene transfer into human hematopoietic progenitor cells.
Gene transfer into hematopoietic progenitor cells is increased when these cells are adherent to the carboxy-terminal chymotryptic fragment of human plasma fibronectin (FN30/35) containing the heparin binding domain (HBD) and the alternatively spliced type three connecting segment (IIICS) region. We report herein the production of a recombinant human fibronectin fragment comprised of the HBD and IIICS regions expressed by Sf9 insect cells following recombinant baculovirus infection. The resulting isolated peptide, HuBacFN, facilitated gene transfer into human hematopoietic cell lines and primary human hematopoietic progenitors to a level achieved with the purified human plasma FN30/35 peptide.
Dutt
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P
Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, USA.
Hanenberg
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H
Vik
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T
Williams
D A
DA
Yoder
M C
MC
D001483:Base Sequence; D005353:Fibronectins / Q000737:chemistry / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics*; D017354:Point Mutation; D011994:Recombinant Proteins / Q000235:genetics; D012190:Retroviridae
D005353:Fibronectins; D010446:Peptide Fragments; D011994:Recombinant Proteins