Examination of the intron in the meiosis-specific recombination gene REC114 in Saccharomyces.
REC114 is one of 10 genes known to be required for the initiation of meiotic recombination in Saccharomyces cerevisiae. It is transcribed only in meiosis, and our previous sequence analysis suggested the presence of an intron in the 3' end of the gene. Hypotheses in the literature have suggested, because of its unusual location, either that the putative intron in REC114 is likely to be necessary for expression, or that there may actually be no intron present. This work demonstrates that REC114 does have an intron and is one of only three genes in yeast with introns located in the 3' end. Furthermore, the 3' splice site utilized in REC114 is a very rare AAG sequence; only three other genes in yeast use this nonconsensus sequence. The splicing of REC114 does not require MER1, a gene known to be involved in meiosis-specific RNA processing. In fact, an intronless copy of REC114 can complement a null rec114 mutation. Thus, it does not appear that the intron is essential for expression of REC114. Although the intron is not absolutely required for meiotic function, it is conserved in evolution; two other species of yeast contain an intron at the same location in their REC114 genes.
Malone
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RE
Dept. of Biological Sciences, University of Iowa, Iowa City 52240, USA.
Pittman
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DL
Nau
J J
JJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D007438:Introns; D008540:Meiosis; D008969:Molecular Sequence Data; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000502:physiology; D045522:Recombinases; D012440:Saccharomyces / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D005656:Fungal Proteins; C092601:MER1 protein, S cerevisiae; C080622:REC114 protein, S cerevisiae; D016601:RNA-Binding Proteins; D045522:Recombinases; D029701:Saccharomyces cerevisiae Proteins