Mapping of peptides and protein fragments in human urine using liquid chromatography-mass spectrometry.
A method for the mapping of peptide mixtures, heterogeneous with respect to the concentration and the size of individual peptides, was established with the aim of obtaining a comprehensive analysis of human urine peptides. Peptide extraction and fractionation were optimized to achieve a two-step analysis, using reversed-phase and ion-exchange chromatography. Highly sensitive detection of peptides was performed by coupling microbore HPLC with electrospray mass spectrometry (ESI-MS). Peptides such as urodilatin, angiotensin and fragments of psoriasin, granulin and uromodulin were isolated and sequenced. The procedure presented here is a tool for the analysis of complex peptide mixtures from human urine.
Heine
G
G
Lower Saxony Institute for Peptide Research, Hannover, Germany.
Raida
M
M
Forssmann
W G
WG
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000652:urine*; D010449:Peptide Mapping / Q000379:methods*; D017421:Sequence Analysis; D013056:Spectrophotometry, Ultraviolet
D010446:Peptide Fragments