[Effects of blastocyst deficiencies induced by aspirin treatment during preimplantation period in rats on development of embryos after implantation].
Pregnant rats were treated orally with aspirin 0.5 or 1.0 g.kg-1 on d 3 (positive vaginal smear was considered as d 0) and were sacrificed on d 4. Some blastocysts collected on d 4 were evaluated for gross morphology and cell number, and the remainings were transfered into pseudopregnant rats. Results showed that the rate of blastocysts with abnormal morphology were 23.8% and 40.8%, respectively, in 0.5 and 1.0 g.kg-1 of aspirin. These were significantly higher than 6.8% of the control group. The cell number of blastocysts also decreased in the aspirin groups. The rate of implantation and live fetuses in the case of blastocysts with normal morphology were related negatively with the aspirin doses, espesially in the group of 1.0 g.kg-1 of aspirin, the implantation rate was significantly lower (38.6%). However, the implantation rate of blastocysts with abnormal morphology in both groups of aspirin were much less than that of the control group, and all embryos after implantation were resorped. No significant malformations were observed in the live fetuses. These results suggests that the effects of blastocyst deficiencies induced by aspirin on development of embryos transfered into pseudopregnant rats mainly caused death of embryos, but not malformation of fetuses.
Ying
Y
Y
Department of Pharmacology, School of Pharmacy, Zhejiang Medical University, Hangzhou.
Cai
Y X
YX
Lou
Y J
YJ
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D001241:Aspirin / Q000633:toxicity*; D001755:Blastocyst / Q000187:drug effects* / Q000473:pathology; D010064:Embryo Implantation / Q000187:drug effects*; D020964:Embryo Loss / Q000139:chemically induced; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001241:Aspirin