Cloning and characterization of a new silver-stainable protein SSP29, a member of the LRR family.
Silver-stainable proteins (SSPs) are aspartic acid-rich nuclear proteins which are silver stained under very specific conditions. Using a degenerate oligodeoxyncleotide probe which codes for acidic amino acid residues, a cDNA for a new SSP, referred to as SSP29, has been isolated. The cDNA-derived amino acid sequence shows SSP29 has a molecular mass of 29 kDa, leucine-rich repeats (LRR) near the NH2-terminal region and acidic clusters at the COOH-terminal portion, indicating that SSP29 is also a member of the LRR subfamily of acidic proteins which have been shown to be involved in antigen-mediated cellular responses, leukemogenesis and differentiation. SSP29 can be stained by Ag-NOR staining. SSP29 is expressed in all human tissues and cell lines tested, localized to nucleoplasm and translocated partially to the nucleoli after heat shock. Its interaction with RNA polymerase I suggests that SSP29 may participate in signal transduction that directs nucleolar activities by regulating ribosomal RNA biosynthesis.
Zhu
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Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA. bvaldez@bcm.tmc.edu
Perlaky
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Henning
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Valdez
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D000090263:Leucine-Rich Repeat Proteins; D008969:Molecular Sequence Data; D009479:Neuropeptides; D009687:Nuclear Proteins / Q000235:genetics* / Q000302:isolation & purification; D011506:Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012834:Silver; D013194:Staining and Labeling
C490341:ANP32B protein, human; D018076:DNA, Complementary; D000090263:Leucine-Rich Repeat Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D011506:Proteins; D012834:Silver