title: Allelic variation in Streptococcus pneumoniae autolysin (N-acetyl muramoyl-L-alanine amidase).
abstract: The lytA gene encoding the autolysin of Streptococcus pneumoniae may be a virulence determinant. Single-strand conformational polymorphism analysis demonstrated heterogenicity throughout the gene in clinical isolates and strains from the clonal serotypes 7 and 14. Sequence analysis of part of the choline-binding domain showed that in two isolates four amino acid substitutions occurred.
authors.affiliation: Department of Medical Microbiology, The Royal Free Hospital School of Medicine, London, United Kingdom. stepheng@rfhsm.ac.uk
authors.forename: S H
authors.initials: SH
authors.lastname: Gillespie
authors.forename: T D
authors.initials: TD
authors.lastname: McHugh
authors.forename: H
authors.initials: H
authors.lastname: Ayres
authors.forename: A
authors.initials: A
authors.lastname: Dickens
authors.forename: A
authors.initials: A
authors.lastname: Efstratiou
authors.forename: G C
authors.initials: GC
authors.lastname: Whiting
mesh_terms: D000483:Alleles; D014644:Genetic Variation; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D013296:Streptococcus pneumoniae / Q000235:genetics*
chemical_list: C076332:EJL amidase; D009238:N-Acetylmuramoyl-L-alanine Amidase