title: Protein kinase C in central vestibular, cerebellar, and precerebellar pathways of the rat.
abstract: The protein kinase C family of enzymes is composed of at least ten different isoforms that display a variety of distinct biochemical specificities. Many of these isoforms are highly expressed in brain, and some show regional specificity in their distribution, suggesting that they may serve specific functions. By using immunocytochemistry to localize the betaI, betaII, gamma, or delta isoforms of protein kinase C in the central vestibular system of the adult rat, we found the vestibular ganglion and its peripheral and central processes of the eighth nerve to be heavily labeled with protein kinase C betaI immunoreactivity. Labeled axons and terminals were also found in all four vestibular nuclei. Some neurons of the vestibular ganglion were weakly stained with the antibody to protein kinase C betaII, as were scattered axons in the eighth nerve, and scattered axons and terminals were found in all four vestibular nuclei among weakly labeled neurons. A few axons in the vestibular portion of the eighth nerve were labeled with protein kinase C gamma immunoreactivity, and neurons of the spinal, lateral, and superior vestibular nuclei were heavily decorated with synapses, presumably derived from Purkinje neurons, which were also strongly immunoreactive. Neurons of the medial vestibular nucleus were not as heavily innervated. With the antibody to protein kinase C delta, we found scattered, weakly immunoreactive neurons in the vestibular portion of the eighth nerve. Myelinated fiber bundles of the spinal vestibular nucleus contained moderate numbers of labeled axons, and the other vestibular nuclei were well innervated by protein kinase C delta axons and terminals. Most of these probably derive from Purkinje cells, which were labeled in longitudinal bands interspersed with bands of labeled basket cells. These data suggest that particular protein kinase C isoforms play specific roles in vestibular and cerebellar function.
authors.affiliation: Department of Otolaryngology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA. mgarcia@mailhost.tcs.tulane.edu
authors.forename: M M
authors.initials: MM
authors.lastname: Garcia
authors.forename: R E
authors.initials: RE
authors.lastname: Harlan
mesh_terms: D000818:Animals; D002529:Cerebellar Nuclei / Q000201:enzymology; D002531:Cerebellum / Q000201:enzymology*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009434:Neural Pathways / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D011689:Purkinje Cells / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014726:Vestibular Nuclei / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C