title: Interleukin-10 reduces the systemic inflammatory response in a murine model of intestinal ischemia/reperfusion.
abstract: BACKGROUND
  Intestinal ischemia/reperfusion (I/R) is known to increase systemic cytokine levels, as well as to activate neutrophils in distant organs. This study was designed to investigate the effect of interleukin-10 (IL-10) on cytokine release, pulmonary neutrophil accumulation, and histologic changes in a murine model of I/R.
  
  
  METHODS
  Forty female Swiss-Webster mice were divided into four groups. Group 1 underwent 45 minutes of superior mesenteric artery occlusion followed by 3-hour reperfusion (I/R). Group 2 underwent laparotomy alone (Sham). Group 3 underwent I/R, but was treated with IL-10, 10,000 units IP every 2 hours, starting 1 hour before reperfusion (Pretreatment). Group 4 was treated with an equal dose of IL-10, starting 1 hour after reperfusion (Posttreatment). All animals were killed at 3 hours, standard assays were performed for serum cytokine levels, and lung myeloperoxidase activity and intestinal histology were scored.
  
  
  RESULTS
  Serum cytokines (TNF-alpha and IL-6), lung myeloperoxidase levels, and histologic score were significantly reduced when IL-10 was administered either before or after reperfusion.
  
  
  CONCLUSIONS
  IL-10 reduced the severity of local and systemic inflammation in a murine model of intestinal I/R when given before or after reperfusion injury. These observations suggest that IL-10 may exert its effect by blocking cytokine production and distant organ neutrophil accumulation.
authors.affiliation: Department of Surgery, UCLA Medical Center 90095, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Lane
authors.forename: K E
authors.initials: KE
authors.lastname: Todd
authors.forename: M P
authors.initials: MP
authors.lastname: Lewis
authors.forename: B
authors.initials: B
authors.lastname: Gloor
authors.forename: S W
authors.initials: SW
authors.lastname: Ashley
authors.forename: H A
authors.initials: HA
authors.lastname: Reber
authors.forename: D W
authors.initials: DW
authors.lastname: McFadden
authors.forename: C F
authors.initials: CF
authors.lastname: Chandler
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007249:Inflammation / Q000209:etiology / Q000517:prevention & control*; D016753:Interleukin-10 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D007511:Ischemia / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D007583:Jejunum / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D008168:Lung / Q000503:physiopathology; D017538:Mesenteric Artery, Superior / Q000502:physiology; D051379:Mice; D009504:Neutrophils / Q000502:physiology; D015427:Reperfusion Injury / Q000276:immunology / Q000517:prevention & control*
chemical_list: D016207:Cytokines; D016753:Interleukin-10