title: Liver microsomal membrane fluidity and microsomal desaturase activities in adult spontaneously hypertensive rats.
abstract: OBJECTIVE
  The purpose of the present study was to investigate liver microsomal membrane fluidity simultaneously with membrane fatty acid composition and desaturase activities in spontaneously hypertensive rats (SHR).
  
  
  METHODS
  The membrane fluidity was determined, after electron spin resonance (ESR) measurement, in SHR compared with normotensive Wistar-Kyoto (WKY) rats, by calculating the order parameter S from ESR spectra of 5-nitroxide stearate and 10-nitroxide stearate, used as spin-labelled fatty acids. Desaturase activities were measured by incubating SHR and WKY rat liver microsomes with [14C]-radiolabeled fatty acids as substrates for desaturation reactions. The fatty acid composition of liver microsomal membranes was determined by gas-liquid chromatography.
  
  
  RESULTS
  Whereas no significant difference between S of 5-nitroxide stearate was observed for SHR and WKY rats, S of 10-nitroxide stearate was significantly lower in SHR than it was in WKY rat microsomal membrane, indicating that the core microsomal membrane fluidity was higher in SHR. Significant differences between fatty acid compositions were observed for SHR and WKY rat microsomal membranes. Delta9 and n-6 delta6 microsomal desaturase activities were significantly lower in SHR.
  
  
  CONCLUSIONS
  These results suggest that the higher liver core microsomal membrane fluidity observed in SHR might be dependent on the increased proportion of mono-unsaturated fatty acids. Such observed modifications and the alterations in delta9 and n-6 delta6 desaturase activities suggest that an impaired polyunsaturated fatty acid biosynthesis is related to changes in microsomal membrane fluidity in hypertension.
authors.affiliation: Unité de Nutrition Cellulaire et Métabolique, EA DGRT 1867, Université de Bourgogne, Faculté des Sciences, Dijon, France.
authors.forename: C
authors.initials: C
authors.lastname: Foucher
authors.forename: M
authors.initials: M
authors.lastname: Narce
authors.forename: L
authors.initials: L
authors.lastname: Nasr
authors.forename: M C
authors.initials: MC
authors.lastname: Delachambre
authors.forename: J P
authors.initials: JP
authors.lastname: Poisson
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D007425:Intracellular Membranes / Q000378:metabolism*; D008297:Male; D008560:Membrane Fluidity; D008862:Microsomes, Liver / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases