Effect of arachidonate on lipid metabolism in ethanol-treated rats fed with lard.
Two groups of rats, ethanol-treated and sucrose-administered control rats, were fed diets with different AA content (0, 2 and 3% weight) for 14 days. Ethanol was administered by gavage at a single daily dose of 3 g/kg body weight. The ethanol-treated rats showed significantly higher levels (p < 0.01) of serum ALT activity. The dietary AA supplement lowered the serum ALT activity and liver triglyceride both in control and ethanol-treated rats. Significantly lower levels of 20:4n - 6 and 20:4n - 6/18:2n - 6 ratio and higher levels of 18:1n - 9 in both the serum and liver triglyceride were observed in the ethanol-treated rats. The AA-supplemented diet induced a marked increase of 20:4n - 6 and subsequent significant decrease of 18:2n - 6 both in the liver and serum phospholipid in control and ethanol-treated rats. 18:1n - 9 in the serum and liver triglyceride in both groups was also markedly decreased by AA supplement. No significant difference was observed in the liver 6-keto-PGF(1 alpha) level between the ethanol-treated and control rats. In the ethanol-treated rats, the level of 6-keto-PGF(1 alpha) was elevated in the rats fed the 3% AA-supplemented diet. Though the liver leukotriene B4 levels were increased by ethanol administration in all rats, these levels were not increased by dietary AA.
Okita
M
M
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Soja Japan.
Suzuki
K
K
Sasagawa
T
T
Yamamoto
J
J
Miyamoto
A
A
Wakabayashi
H
H
Watanabe
A
A
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D001835:Body Weight; D004041:Dietary Fats / Q000008:administration & dosage*; D000431:Ethanol / Q000494:pharmacology*; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism; D005247:Feeding Behavior; D007975:Leukotriene B4 / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley
D004041:Dietary Fats; D005227:Fatty Acids; D008055:Lipids; D007975:Leukotriene B4; D016718:Arachidonic Acid; D000431:Ethanol; D015121:6-Ketoprostaglandin F1 alpha; D000410:Alanine Transaminase; C029310:lard