Prevention of parenteral nutrition-induced gut hypoplasia by coinfusion of glucagon-like peptide-2.
Maintaining rats on total parenteral nutrition (TPN) for 6 days significantly reduced mass (-34%), protein (-32%), and DNA (-35%) in small intestine and colon (29-37% decrease). Coinfusion of glucagon-like peptide-2 (GLP-2) normalized each of these variables in duodenum, jejunum, and ileum, but not in colon. Histological analysis of tissue revealed normal mucosa thickness and villus height in small intestine of GLP-2-treated rats, whereas nontreated rats maintained on TPN exhibited villus shortening (-30%) and thinning (-23%) of mucosa. These results suggest that hormonal alterations may be more important than an absence of luminal nutrition in TPN-associated mucosal changes. Additionally, GLP-2 normalization of gut mucosa permits accurate assessment of the influence of reversal of hypoplasia on gut barrier function.
Chance
W T
WT
Medical Research Service, Department of Veterans Affairs, Cincinnati, Ohio, USA.
Foley-Nelson
T
T
Thomas
I
I
Balasubramaniam
A
A
D000818:Animals; D004247:DNA / Q000378:metabolism; D053767:Glucagon-Like Peptide 2; D004763:Glucagon-Like Peptides; D007410:Intestinal Diseases / Q000209:etiology* / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000473:pathology; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology; D008297:Male; D008871:Microvilli / Q000473:pathology; D010288:Parenteral Nutrition / Q000009:adverse effects*; D010455:Peptides / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344
D053767:Glucagon-Like Peptide 2; D010455:Peptides; D004763:Glucagon-Like Peptides; D004247:DNA