A novel zebrafish gene expressed specifically in the photoreceptor cells of the retina.
We have identified and characterized a novel protein from adult zebrafish retina, which we named ES1. Database search revealed that the ES1 gene has significant similarity to two genes with unknown functions: the Escherichia coli sigma cross-reacting protein 27a (scrp27a) and the human KNP-I/GT335. In situ hybridization and immunohistochemistry experiments showed that both ES1 mRNA and protein are expressed specifically in adult photoreceptor cells. ES1 seems to be a cytoplasmic protein. An ES1-like antigen was also detected in photoreceptor cells of goldfish with anti-ES1 antibodies. The retina specific expression and the evolutionary conservation suggest that ES1 protein may be important for maintaining normal retina structure and function.
Chang
H
H
Biophysics Program, Harvard University, Cambridge, Massachusetts 02138, USA. hanchang@leland.stanford.edu
Gilbert
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D051792:Basic Helix-Loop-Helix Proteins; D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005136:Eye Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D018398:Homeodomain Proteins; D006801:Humans; D007150:Immunohistochemistry; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000072056:Transcription Factor HES-1; D014158:Transcription, Genetic; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins
D001426:Bacterial Proteins; D051792:Basic Helix-Loop-Helix Proteins; D029968:Escherichia coli Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000072056:Transcription Factor HES-1; D029961:Zebrafish Proteins; C107626:ES1 protein, zebrafish; C101148:elbB protein, E coli; C078493:HES1 protein, human