Co-expression and secretion of C3, the third component of complement and a C3-cleaving cysteine proteinase in a highly metastatic human melanoma cell line.
We recently demonstrated that DM-4, a human melanoma cell line highly metastatic in nude mice, expressed a p41 C3-cleaving proteinase. This p41 proteinase is a cysteine proteinase, associated to cell surface and involved in tumorigenicity and metastatic properties of these tumor cells. We demonstrate herein that DM-4 cells also secrete the p41 proteinase. In addition, analysis of cellular components which reacted with the p41 proteinase led us to demonstrate that DM-4 cells synthesized and secreted human C3. Secreted C3 is cleaved by the secreted p41 proteinase and a C3dg-like fragment is generated. This is the first demonstration that a human melanoma cell line co-expresses and co-secretes human C3 and a C3-cleaving cysteine proteinase, antigenically related to procathepsin L.
Jean
D
D
Immunochimie des Régulations Cellulaires et des Interactions Virales, INSERM U.354, Centre INSERM, Hôpital Saint-Antoine, Paris, France.
Rodrigues-Lima
F
F
Cassinat
B
B
Hermann
J
J
Cabane
J
J
Frade
R
R
D000818:Animals; D056668:Cathepsin L; D002403:Cathepsins / Q000276:immunology; D003176:Complement C3 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004792:Enzyme Precursors / Q000276:immunology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D003176:Complement C3; D004792:Enzyme Precursors; D009363:Neoplasm Proteins; D002403:Cathepsins; C097554:C3-cleaving proteinase, 39 kDa; D003546:Cysteine Endopeptidases; C063905:procathepsin L; D056668:Cathepsin L