title: Recognition of dengue virus NS1-NS2a proteins by human CD4+ cytotoxic T lymphocyte clones.
abstract: We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-attenuated experimental dengue-1 vaccine. Of nine CD4+ T cell clones, seven were dengue-1-specific and two were dengue-1-dengue-3 cross-reactive. Four dengue-1-specific clones and one dengue-1-dengue-3 cross-reactive clone recognized epitopes within the NS1 or NS2a proteins. Analysis of human leukocyte antigen (HLA) restriction revealed that three dengue-1-specific clones are HLA-DR1-restricted and one dengue-1-dengue-3 cross-reactive clone is HLA-DPw3-restricted. These results indicate that NS1 and NS2a proteins as well as C, E, and NS3 proteins reported earlier contain one or more epitopes recognized by dengue virus-specific human CD4+ T lymphocytes.
authors.affiliation: Department of Medicine, University of Massachusetts Medical Center, Worcester 01655, USA.
authors.forename: S
authors.initials: S
authors.lastname: Green
authors.forename: I
authors.initials: I
authors.lastname: Kurane
authors.forename: S
authors.initials: S
authors.lastname: Pincus
authors.forename: E
authors.initials: E
authors.lastname: Paoletti
authors.forename: F A
authors.initials: FA
authors.lastname: Ennis
mesh_terms: D000956:Antigens, Viral / Q000276:immunology; D015704:CD4 Antigens; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D006801:Humans; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D015704:CD4 Antigens; D017361:Viral Nonstructural Proteins