Cloning, human chromosomal assignment, and adipose and hepatic expression of the CL-6/INSIG1 gene.
Rat CL-6 is the most highly insulin-induced gene in a liver cell line and is expressed in proliferating liver during regeneration and development. CL-6 is now denoted INSIG1 (insulin-induced gene 1). Human INSIG1 was isolated and found to be 80% identical to the rat gene within the translated region. It was located on human chromosome 7 within band q36. The human INSIG1 promoter conferred a high level of expression in both liver and fibroblast cell lines. INSIG1 expression was upregulated at the transcriptional level in rat regenerating liver and induced in a model of murine adipocyte differentiation, suggesting that INSIG1 may play a role in growth and differentiation of tissues involved in metabolic control.
Peng
Y
Y
Department of Genetics, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, 19104, USA.
Schwarz
E J
EJ
Lazar
M A
MA
Genin
A
A
Spinner
N B
NB
Taub
R
R
D000273:Adipose Tissue / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D003001:Cloning, Molecular; D015870:Gene Expression / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D005796:Genes / Q000235:genetics; D015698:Genomic Library; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D008115:Liver Regeneration / Q000235:genetics / Q000502:physiology; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000502:physiology; D011506:Proteins / Q000235:genetics*; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
C108626:INSIG1 protein, human; C491574:Insig1 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011506:Proteins