Expression of an alpha-cardiac like myosin heavy chain in diaphragm, chronically stimulated, and denervated fast-twitch muscles of rabbit.
An additional slow fibre type, type I alpha, is detected in diaphragm and appears in fast-twitch hindlimb muscles of rabbit under the influence of altered neuromuscular activity. Type I alpha fibres were delineated from fibres expressing myosin heavy chain I beta (type I beta) by immunohistochemistry with a monoclonal antibody raised against the alpha-cardiac MHCI alpha. When stained for mATPase after acid and alkaline preincubations, some type I alpha fibres resembled type I beta and type IIA fibres, respectively. Some type I alpha fibres displayed dissimilar mATPase staining, indicating heterogeneity of this fibre population. The appearance of numerous type I alpha fibres in stimulated muscles, which in addition contain type IIA and type I beta fibres, suggested that they may be interspaced between types IIA and I beta. Electrophoresis under nondenaturing conditions disclosed an additional isomyosin both in normal diaphragm and stimulated muscles. This band displayed the same mobility as the slowest isomyosin in rabbit masseter muscle. It was recognized by the same monoclonal (anti-alpha-cardiac MHC) antibody used for immunohistochemistry. Therefore, this isomyosin appeared to be very similar, but perhaps not identical to the alpha-cardiac MHC-based isomyosin, probably resulting from discrete differences in the MHC complement. This assumption agrees with additional findings suggesting an even greater heterogeneity of the MHCs than generally assumed. In support of this, we show in atrium and masseter muscles the existence of an additional, electrophoretically distinct MHC isoform which migrates in close vicinity to MHCI alpha.
Hämäläinen
N
N
Fakultat für Biologie, Universität Konstanz, Germany.
Pette
D
D
D000818:Animals; D024722:Cardiac Myosins; D003964:Diaphragm / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008297:Male; D009121:Muscle Denervation; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology; D018995:Myosin Heavy Chains; D009218:Myosins / Q000032:analysis* / Q000096:biosynthesis; D011817:Rabbits
C094239:MYH6 protein, human; D024722:Cardiac Myosins; D018995:Myosin Heavy Chains; D009218:Myosins