title: In vitro determination of ruminal protein degradability of alfalfa and prairie hay via a commercial protease in the presence or absence of cellulase or driselase.
abstract: Ruminal protein degradation of alfalfa (2.62% N, 49.6% NDF, and in vivo undegradable intake protein [UIP] = 16.4% of CP) and prairie hay (.88% N, 69.4% NDF, and in vivo UIP = 44.5% of CP) was estimated using the Streptomyces griseus protease (SGP) in vitro method with or without pretreatment with two carbohydrases: cellulase from Penicillium funiculosum or driselase from Basidiomycetes. Driselase is a broad-spectrum carbohydrase. Incubating forage samples for 48 h with cellulase or driselase at a concentration of 800 mg/g per g of hay nearly maximized ADF and NDF disappearances. This concentration and incubation time then were used to pretreat hay samples. A 2-h pretreatment was included to evaluate the potential for reducing the analysis time. Other sets of samples were or were not pretreated with acetate buffer alone. Following pretreatment, samples were subjected to SGP for .25, .5, 1, 2, 4, 8, 12, 24, and 48 h. Pretreatment altered the sizes of protein pools and their degradation rates. When the UIP contents of the forages were estimated using SGP and a single-pool, first-order, kinetic model, cellulase (48 h) or driselase pretreatments yielded UIP predictions that were more similar to in vivo values. Some carbohydrase and protease combinations also yielded single time-point estimates of UIP that were similar to in vivo values. Similarly, when sufficient time was permitted for protease incubation, single time-point estimates derived from protease alone were similar to in vivo values.
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506, USA.
authors.forename: I E
authors.initials: IE
authors.lastname: Abdelgadir
authors.forename: R C
authors.initials: RC
authors.lastname: Cochran
authors.forename: E C
authors.initials: EC
authors.lastname: Titgemeyer
authors.forename: E S
authors.initials: ES
authors.lastname: Vanzant
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000201:enzymology; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism / Q000494:pharmacology*; D004044:Dietary Proteins / Q000032:analysis / Q000378:metabolism*; D004063:Digestion / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D010450:Endopeptidases / Q000378:metabolism / Q000494:pharmacology*; D005656:Fungal Proteins; D006026:Glycoside Hydrolases / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D016014:Linear Models; D000455:Medicago sativa / Q000737:chemistry / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology; D006109:Poaceae / Q000737:chemistry / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism* / Q000502:physiology; D013305:Streptomyces griseus / Q000201:enzymology
chemical_list: D004044:Dietary Proteins; D005656:Fungal Proteins; D006026:Glycoside Hydrolases; C035212:driselase; D002480:Cellulase; D010450:Endopeptidases