title: Regulated nuclear translocation of the Mig1 glucose repressor.
abstract: Glucose represses the transcription of many genes in bakers yeast (Saccharomyces cerevisiae). Mig1 is a Cys2-His2 zinc finger protein that mediates glucose repression of several genes by binding to their promoters and recruiting the general repression complex Ssn6-Tup1. We have found that the subcellular localization of Mig1 is regulated by glucose. Mig1 is imported into the nucleus within minutes after the addition of glucose and is just as rapidly transported back to the cytoplasm when glucose is removed. This regulated nuclear localization requires components of the glucose repression signal transduction pathway. An internal region of the protein separate from the DNA binding and repression domains is necessary and sufficient for glucose-regulated nuclear import and export. Changes in the phosphorylation status of Mig1 are coincident with the changes in its localization, suggesting a possible regulatory role for phosphorylation. Our results suggest that a glucose-regulated nuclear import and/or export mechanism controls the activity of Mig1.
authors.affiliation: Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: De Vit
authors.forename: J A
authors.initials: JA
authors.lastname: Waddle
authors.forename: M
authors.initials: M
authors.lastname: Johnston
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D005800:Genes, Fungal / Q000187:drug effects* / Q000502:physiology; D005947:Glucose / Q000494:pharmacology*; D010766:Phosphorylation; D010957:Plasmids; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D004268:DNA-Binding Proteins; C085976:MIG1 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D005947:Glucose