Isoform-specific monoclonal antibodies against HSP90.
The purpose of this study was to develop monoclonal antibodies (mAbs) that distinguish between the two isoforms of human 90-kDa heat shock protein (HSP90), i.e., HSP90 alpha and beta. Human HSP90 alpha and beta isoforms expressed in Escherichia coli were separately used as antigens for developing the mAbs. Twenty-three and ten mAbs were obtained by immunization of mice with HSP90 alpha and beta, respectively. Among them, ten and three mAbs specifically recognized HSP90 alpha and beta isoforms, respectively, on the criteria of both enzyme-linked immunosorbent and immunoblotting analyses. Immunochemical analysis by use of the mAbs revealed that both of the HSP90 isoforms were present in human cells even under unstressed conditions and that the expression of HSP90 alpha was more strongly induced when the cells were exposed to arsenate. This is the first report of the development of the mAbs discriminating between the two isoforms of HSP90. The mAbs specific for HSP90 isoforms should be useful for the regulational and functional analyses of HSP90 isoforms.
Nemoto
T
T
Department of Biochemistry, Iwate Medical University School of Dentistry, Morioka, Japan. tnemoto@iwate-med.ac.jp
Roi
R
R
Matsusaka
T
T
Iwanari
H
H
Yamashita
H
H
Kyakumoto
S
S
Sato
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000276:immunology*; D018882:Heat Stress Disorders; D006801:Humans; D015151:Immunoblotting; D016056:Immunodominant Epitopes; D051379:Mice
D000911:Antibodies, Monoclonal; D018841:HSP90 Heat-Shock Proteins; D016056:Immunodominant Epitopes