Inhibition of leukocyte leukotriene B4 production by an olive oil-derived phenol identified by mass-spectrometry.
We have evaluated the effects of hydroxytyrosol (HT), a potent antioxidant present in olive oil, on the formation of arachidonic acid 5-lipoxygenase metabolites by leukocytes in vitro. HT, a simple phenolic compound, extracted from first-pressure oil, was isolated by HPLC and characterized by gas chromatography/mass spectrometry. HT inhibited in a dose-related manner the production of leukotriene B4 (LTB4) by calcium ionophore-stimulated leukocytes. As expected, similar inhibition was observed for omega-oxidized metabolites of LTB4, namely 20-hydroxy and 20-carboxy-LTB4. The results disclose a new biological activity of olive oil-derived phenols on leukocyte eicosanoid production.
Petroni
A
A
Institute of Pharmacological Sciences, University of Milan, Italy. petroni@isfunix.farma.unimi.it
Blasevich
M
M
Papini
N
N
Salami
M
M
Sala
A
A
Galli
C
C
D000975:Antioxidants / Q000494:pharmacology*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D002118:Calcium / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D003864:Depression, Chemical; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D007975:Leukotriene B4 / Q000096:biosynthesis*; D000069463:Olive Oil; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry*
D000975:Antioxidants; D007476:Ionophores; D000069463:Olive Oil; D010938:Plant Oils; C005975:3,4-dihydroxyphenylethanol; D007975:Leukotriene B4; D001094:Arachidonate 5-Lipoxygenase; D010626:Phenylethyl Alcohol; D002118:Calcium