Urease-induced crystallizations of calcium phosphate and magnesium ammonium phosphate in synthetic urine and human urine.
An aggregometer technique was used to study urease-induced crystallizations in synthetic urine and human urine from healthy subjects and patients with chronic spinal cord injuries. The two different phases of crystallization, calcium phosphate and magnesium ammonium phosphate, were easily evaluated with a single assay using this technique. The crystallization of calcium phosphate and magnesium ammonium phosphate varied markedly among the different urine specimens after incubation with urease. The turbidity curves from human urine were divided into four patterns. We assumed that the variations in the patterns of the turbidity curves appeared to be mainly due to differences in the composition of the urine and in the original pH, and that the calcium and magnesium concentrations were very important in the urinary constituents.
Ebisuno
S
S
Division of Urology, Minami Wakayama Hospital, Tanabe shi, Japan.
Komura
T
T
Yamagiwa
K
K
Ohkawa
T
T
D002130:Calcium Phosphates / Q000737:chemistry / Q000652:urine*; D003460:Crystallization; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017616:Magnesium Compounds / Q000737:chemistry / Q000652:urine*; D008297:Male; D010710:Phosphates / Q000737:chemistry / Q000652:urine*; D013119:Spinal Cord Injuries / Q000652:urine; D000069877:Struvite; D014510:Urease / Q000737:chemistry / Q000652:urine*
D002130:Calcium Phosphates; D017616:Magnesium Compounds; D010710:Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; D000069877:Struvite; D014510:Urease; C485829:calcium phosphate, dibasic, anhydrous