title: Genome analysis in Neurospora crassa; cloning of four loci arginine-1 (arg-1), methionine-6 (met-6), unknown-7 (un-7), and ribosome production-1 (rip-1) and associated chromosome walking.
abstract: We have cloned four Neurospora crassa genes by complementation analysis. Cloned genes include the arginine-1 (arg-1), methionine-6 (met-6), unknown-7 (un-7), and ribosome production-1 (rip-1) loci. Chromosome walks were initiated in ordered cosmid libraries from the cloned loci. A total of about 700 kb of the Neurospora genome is covered in these walks.
authors.affiliation: Department of Biology, University of Southwestern Louisiana, Lafayette 70504-2451, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Schmidhauser
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Zhou
mesh_terms: D001120:Arginine / Q000235:genetics*; D002874:Chromosome Mapping; D016386:Chromosome Walking; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular / Q000379:methods; D003360:Cosmids; D015723:Gene Library; D005819:Genetic Markers; D016681:Genome, Fungal; D008715:Methionine / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012270:Ribosomes
chemical_list: D005819:Genetic Markers; D001120:Arginine; D008715:Methionine