[Distribution and quantification of oxidative low density lipoprotein in the development of atherosclerotic lesions of human aorta].
A total of 35 human aorta specimens obtained within 12 hours postmortem were collected. The distribution pattern and quantity of LDL and ox-LDL in atherosclerotic lesions of varying severity and the relationship with aorta cells were studied by immunohistochemical techniques and computorized image processing. The results showed that the staining pattern with anti-ox-LDL and antibodies to apoB was similar; in fatty steaks, the staining for ox-LDL was predominantly cell-associated in macrophages derived foam cells, in the late lesions, its pattern shifted to smooth muscle cells and extra-cellular matrix staining, there was also more apoB in fibrofatty plaques and atheromatous plaques than in fatty streaks, but no significant difference was found between the two stages of plaques. The quantity of Cu(2+)-ox-LDL significantly increased with the development of AS. There was more LDL than ox-LDL in fatty streaks, but in the late plaques, there was significantly more ox-LDL than LDL.
Mao
Z
Z
Department of Pathology, Zhejiang Medical University, Hangzhou.
Lou
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D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D005487:Foam Cells / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008077:Lipoproteins, LDL / Q000378:metabolism*
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein