[An autobacteriographic study on distribution and localization of methicillin-resistant Staphylococcus aureus in the immunosuppressed mice].
Cyclophosphamide (CY, 250 mg/kg) was intraperitoneally administered to mice. Four days after, a rifampicin-resistant strain of methicillin-resistant Staphylococcus aureus (MRSA, S. aureus 1-6 RFPr) was intravenously inoculated at the level of 10(7) cfu/mouse. Distribution and localization of the inoculated organism were chronologically investigated by means of whole body autobacteriography. CY (100 mg/kg) was consecutively administered for 4 days following the inoculation. As a result, dense colonies of the organism were detected from many organs and tissues, that is, the liver spleen, gastrointestinal tract, kidneys, urinary bladder and bone (bone marrow) on the day after the inoculation. Following 3 days after the inoculation, the distribution and localization in CY-treated mice remained substantially unchanged and some animals died. It is demonstrated that in an experimental mouse model of MRSA infectious disease under immunosuppressed condition, the inoculated organism can stand still and proliferate not only in the gastrointestinal tract but also in the urinary tract and lymphhemopoietic organs.
Tani
K
K
Pharmaceutical Development Research Laboratory, Tanabe Seiyaku Co. Ltd., Saitama, Japan.
Yoshikawa
M
M
D000818:Animals; D001345:Autoradiography; D001853:Bone Marrow / Q000382:microbiology; D015169:Colony Count, Microbial / Q000379:methods*; D003520:Cyclophosphamide / Q000008:administration & dosage; D004064:Digestive System / Q000382:microbiology; D004195:Disease Models, Animal; D016867:Immunocompromised Host; D008297:Male; D016106:Methicillin Resistance; D051379:Mice; D008815:Mice, Inbred Strains; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D014551:Urinary Tract / Q000382:microbiology
D003520:Cyclophosphamide