title: Human histidine-rich glycoprotein expressed in SF9 insect cells inhibits apatite formation.
abstract: Histidine-rich glycoprotein (HRG) is structurally related to the alpha2-HS glycoprotein/fetuin family of mammalian plasma proteins; both belong to the cystatin superfamily of proteins. We expressed recombinant human HRG and alpha2-HS in Sf9 insect cells for functional analysis. Recombinant HRG bound heparin and fibrinogen while alpha2-HS did not. Both proteins inhibited the formation of apatite, recombinant HRG (IC50 approximately 1 microM) with 2-fold lower molar activity than alpha2-HS (IC50 approximately 0.5 microM). The inhibition in vitro of apatite formation suggests a new function for plasma HRG protein, inhibition of phase separation in blood vessels.
authors.affiliation: Institute for Physiological Chemistry and Pathobiochemistry, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Schinke
authors.forename: T
authors.initials: T
authors.lastname: Koide
authors.forename: W
authors.initials: W
authors.lastname: Jahnen-Dechent
mesh_terms: D000818:Animals; D001031:Apatites / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001798:Blood Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism; D002460:Cell Line; D006493:Heparin / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology; D018411:Spodoptera; D060749:alpha-2-HS-Glycoprotein; D000099053:Histidine-Rich Glycoprotein
chemical_list: C556354:AHSG protein, human; D001031:Apatites; D001798:Blood Proteins; D011506:Proteins; D011994:Recombinant Proteins; D060749:alpha-2-HS-Glycoprotein; D000099053:Histidine-Rich Glycoprotein; D006493:Heparin