Differential regulations of matrix metalloproteinases and tissue inhibitors of metalloproteinases in dermal fibroblasts by dibutyril cyclic AMP.
The influence of dibutyril cyclic AMP (dbcAMP) on the gene expression of matrix metalloproteinases (MMP)-1, MMP-2, MMP-3, tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2 was investigated in human skin fibroblasts by northern analyses. The treatment of dbcAMP increased MMP-1 and MMP-3 mRNA levels but decreased TIMP-1 mRNA levels in time and dose dependent manners. Procollagen alpha 1 (I), MMP-2 and TIMP-2 mRNA levels were unaltered by this reagent. Our data indicate that dbcAMP potentially enhances the degradation of extracellular components of connective tissue.
Kuroda
K
K
Department of Dermatology, School of Medicine, Chiba University, Japan.
Shinkai
H
H
D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics; D017364:Collagenases / Q000235:genetics; D005109:Extracellular Matrix / Q000201:enzymology; D005347:Fibroblasts / Q000378:metabolism; D018093:Gelatinases / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D020781:Matrix Metalloproteinase 1; D020778:Matrix Metalloproteinase 2; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000235:genetics*; D011480:Protease Inhibitors / Q000378:metabolism*; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2; D019714:Tissue Inhibitor of Metalloproteinases; D015854:Up-Regulation / Q000187:drug effects
D006023:Glycoproteins; D011480:Protease Inhibitors; D011506:Proteins; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D019716:Tissue Inhibitor of Metalloproteinase-2; D003994:Bucladesine; D003094:Collagen; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1