Characterization of Escherichia coli strains with gapA and gapB genes deleted.
We obtained a series of Escherichia coli strains in which gapA, gapB, or both had been deleted. Delta gapA strains do not revert on glucose, while delta gapB strains grow on glycerol or glucose. We showed that gapB-encoded protein is expressed but at a very low level. Together, these results confirm the essential role for gapA in glycolysis and show that gapB is dispensable for both glycolysis and the pyridoxal biosynthesis pathway.
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Laboratoire d'Enzymologie et de Génie Génétique, Université Henri Poincaré Nancy I, URA CNRS 457, Vandoeuvre-les-Nancy, France.
Boschi-Muller
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Vignais
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Branlant
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D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D005947:Glucose / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D006019:Glycolysis; D010641:Phenotype; D011732:Pyridoxal Phosphate / Q000096:biosynthesis; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
D003470:Culture Media; D029968:Escherichia coli Proteins; D013386:Succinates; D011732:Pyridoxal Phosphate; D019802:Succinic Acid; D000445:Aldehyde Oxidoreductases; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C066236:erythrose 4-phosphate dehydrogenase, E coli; D005947:Glucose; D005990:Glycerol