A simple method for preparation of cultured cardiac fibroblasts from adult human ventricular tissue.
Cardiac fibroblasts constitute greater than 90% of non-myocyte cells in the heart. Because they are responsible for synthesis of components of the extracellular matrix, growth factors and cytokines in the myocardium, they play an important role in normal and pathologic performance of the heart. An understanding of their biology requires in depth studies in a stable and reliable system in which the biological responses of cardiac fibroblasts to various stimuli can be determined. With the exception of few, all studies have been performed on cardiac fibroblasts obtained from rodent hearts. We present a method for isolation and subsequent culture of viable cardiac fibroblasts from ventricular tissue of adult human. This method allows rapid and reliable isolation and subsequent culture of cardiac fibroblasts from adult heart tissue without the need for cumbersome isolation techniques and complex nutrient-enriched and hormone-supplemented culture media for maintenance.
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Department of Anesthesiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Eghbali-Webb
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D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002478:Cells, Cultured; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006352:Heart Ventricles / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D008297:Male; D008858:Microscopy, Phase-Contrast; D009206:Myocardium / Q000737:chemistry / Q000166:cytology