title: Effect of human vitreous and hyalocyte-derived factors on vascular endothelial cell growth.
abstract: OBJECTIVE
  In the present study we have examined the effects of human vitreous and hyalocyte-conditioned medium on human umbilical vein endothelial cells (HUVEC).
  
  
  METHODS
  Human umbilical vein endothelial cells were cultured and treated in quadruplicate with human vitreous and hyalocyte-conditioned medium at different concentrations. Cell numbers were counted on days 1, 3, 5 and 7. Morphological changes and the viability of cells after treatment were also monitored.
  
  
  RESULTS
  The results indicate that both human vitreous and hyalocyte-conditioned medium inhibit proliferation and reduce the viability of HUVEC in vitro. These inhibitory effects were dose- and time-dependent.
  
  
  CONCLUSIONS
  The observations suggest that human hyalocytes and vitreous contain anti-angiogenic factors that influence vascular endothelial cell-growth. These results, combined with those of previous studies, may yield important information about the functional role of vitreous and hyalocytes in intraocular vascular regression.
authors.affiliation: Department of Clinical Ophthalmology, University of Sydney, New South Wales, Australia. meidong@pc-0.eye.usyd.edu.au
authors.forename: M
authors.initials: M
authors.lastname: Zhu
authors.forename: P L
authors.initials: PL
authors.lastname: Penfold
authors.forename: M C
authors.initials: MC
authors.lastname: Madigan
authors.forename: F A
authors.initials: FA
authors.lastname: Billson
mesh_terms: D000328:Adult; D002452:Cell Count; D018929:Cell Culture Techniques; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D016228:Endothelial Growth Factors / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005500:Follow-Up Studies; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology; D014471:Umbilical Veins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014822:Vitreous Body / Q000196:embryology / Q000502:physiology*
chemical_list: D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors