Type 1 HERV-K genome is spliced into subgenomic transcripts in the human breast tumor cell line T47D.
Two types of HERV-K genomes exist which differ in the absence (type 1) or the presence (type 2) of a sequence of 292 nucleotides between the putative pol and env genes. Previously published results from teratocarcinoma cell studies had firmly concluded that the type 1 HERV-K genome was defective in splicing and that only the nondeleted type 2 HERV-K genome containing the 292-nucleotide sequence was capable of being spliced. We now show that in the T47D human breast tumor cell line it is the type 1 HERV-K genome, and not the type 2, which is spliced to subgenomic transcripts.
Etkind
P R
PR
Department of Oncology, Montefiore Medical Center and Albert Einstein College of Medicine, Bronx, New York 10467, USA.
Lumb
K
K
Du
J
J
Racevskis
J
J
D001943:Breast Neoplasms / Q000235:genetics* / Q000821:virology*; D005260:Female; D016679:Genome, Viral; D006801:Humans; D012326:RNA Splicing; D012190:Retroviridae / Q000235:genetics*; D017421:Sequence Analysis; D014407:Tumor Cells, Cultured