The effects of several ecdysteroids and ecdysteroid agonists on two Drosophila imaginal disc cell lines.
Two Drosophila imaginal disc cell lines, C18+ (sensitive to 20-hydroxyecdysone, 20HE) and C18R (resistant to 20HE) were exposed to the ecdysteroid agonists RH5849 and RH5992 and the ecdysteroids inokosterone, makisterone A and muristerone A. All compounds tested were found to have similar effects on the cells, comparable to the effects of 20HE, although at different concentrations. C18R showed resistance to all compounds, again at varying concentrations. We conclude that it is likely that all the compounds tested use the same receptors as 20HE, but show maximum effectiveness at different concentrations.
Cottam
D M
DM
School of Biological and Medical Sciences, University of St. Andrews, Fife, UK.
Milner
M J
MJ
D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002782:Cholestenes / Q000494:pharmacology; D004330:Drosophila / Q000166:cytology* / Q000187:drug effects; D004440:Ecdysone / Q000031:analogs & derivatives / Q000494:pharmacology; D026461:Ecdysteroids; D006834:Hydrazines / Q000494:pharmacology; D007301:Insect Hormones / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology; D013256:Steroids / Q000819:agonists* / Q000494:pharmacology*
D002782:Cholestenes; D026461:Ecdysteroids; D006834:Hydrazines; D007301:Insect Hormones; D007306:Insecticides; D013256:Steroids; C056565:1,2-dibenzoyl-tert-butylhydrazine; C008310:makisterone; C100084:inokosterone; D004440:Ecdysone; C082026:tebufenozide