Thyroid cell transformation inhibits the expression of a novel rat protein tyrosine phosphatase.
We have isolated a rat thyroid cDNA encoding a novel rat receptor-type tyrosine phosphatase protein. This gene, on the basis of its homology to another tyrosine phosphatase, the recently isolated human DEP-1/HPTPeta, has been named r-PTPeta. In rat thyroid cells the r-PTPeta gene acts as a differentiation marker. Indeed, the block of thyroid cell differentiation induced by viral and cellular oncogenes is associated with the inhibition or marked reduction of the expression of this gene, and its expression is positively regulated by thyrotropin, the physiological stimulator of thyroid cell growth.
Zhang
L
L
Facoltà di Medicina e Chirurgia di Catanzaro, Università degli Studi di Reggio Calabria, via Tommaso Campanella 5, Catanzaro, 88100, Italy.
Martelli
M L
ML
Battaglia
C
C
Trapasso
F
F
Tramontano
D
D
Viglietto
G
G
Porcellini
A
A
Santoro
M
M
Fusco
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003062:Codon; D017931:DNA Primers; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009857:Oncogenes; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D011916:Rats, Inbred F344; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013961:Thyroid Gland / Q000166:cytology / Q000201:enzymology*; D013972:Thyrotropin / Q000494:pharmacology; D014158:Transcription, Genetic
D003062:Codon; D017931:DNA Primers; D018076:DNA, Complementary; D013972:Thyrotropin; C486288:PTPRJ protein, human; D017027:Protein Tyrosine Phosphatases; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3