Enantiomeric properties of human albumin immobilized on porous silica supports coated with polymethacryloyl chloride.
Human serum albumin (HSA) was bound to porous silica, using a reactive polymer derived from polymethacryloyl chloride. Two different procedures were used for coating silica with the polymer. In the first method, the polymer was deposited onto amino-silica by reaction between its reactive functions and NH2 groups on silica. In the second method, the monomer was first linked to the amino-silica and copolymerization with the excess of monomer initiated thereafter. The enantiomeric properties of the resulting supports after the coupling of HSA were compared using different mobile phases. The higher amount of HSA bound using the later method, resulted in higher retention of the enantiomers and better enantioselectivity.
Millot
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MC
Laboratoire de Physico-Chimie des Biopolymères, UMR 27, CNRS, Université Paris XII, Thiais, France.
Sébille
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Mangin
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D000345:Affinity Labels; D002021:Buffers; D006801:Humans; D007202:Indicators and Reagents; D010076:Oxazepam / Q000737:chemistry; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000737:chemistry; D011109:Polymethacrylic Acids; D016062:Porosity; D012709:Serum Albumin / Q000737:chemistry*; D012822:Silicon Dioxide; D013237:Stereoisomerism; D014364:Tryptophan / Q000737:chemistry; D014859:Warfarin / Q000737:chemistry
D000345:Affinity Labels; D002021:Buffers; D007202:Indicators and Reagents; D011109:Polymethacrylic Acids; D012709:Serum Albumin; C030613:polymethacrylic acid; D010649:Phenylalanine; D014859:Warfarin; D010076:Oxazepam; D012822:Silicon Dioxide; D014364:Tryptophan; C053764:benzoylphenylalanine