title: Soluble CD16/Fc gamma RIII induces maturation of dendritic cells and production of several cytokines including IL-12.
abstract: Fc gamma RIII (CD16), a low affinity FcR which binds IgG-containing immune-complexes, exists under membrane-associated forms and under a soluble form (sFc gamma RIII). The latter, present in biological fluids (serum, saliva), is generated by proteolytic cleavage of the two membrane-associated Fc gamma RIII isoforms, Fc gamma RIII-A (expressed by macrophages and NK cells) and Fc gamma RIII-B (expressed exclusively by neutrophils). Herein we demonstrate that dendritic cells (DCs), generated by culturing monocytes with GM-CSF and IL-4, bind biotinylated recombinant sFc gamma RIII. This binding is specific and involves the complement receptor CR3 (CD11b/CD18) and CR4 (CD11c/CD18). Indeed, preincubation of DCs with anti-CD11b and anti-CD11c mAbs decreased by 52% and 62% respectively the binding with sFc gamma RIII. Moreover, electron microscopy showed that binding of gold-labeled sFc gamma RIII to DCs maintained at 4 degrees C occurred within clathrin-coated pits. Once internalized, at 37 degrees C, sFc gamma RIII entered the endocytic pathway and reached the MHC class II compartments. Furthermore, DCs incubated for 48 h with multivalent sFc gamma RIII expressed increased levels of CD40, CD80, CD86, CD54, CD58, HLA class I and class II molecules and decreased levels of CD23 and CD32. These effects result in an increased capacity of DCs to trigger proliferative responses by CD4+ CD45RA+ allogeneic T cells. RT-PCR amplification demonstrated that incubation of DCs for 20 h in the presence of multivalent sFc gamma RIII induced the appearance of GM-CSF and IL-12 p40 mRNA. Among the cytokines constitutively expressed, IL-1 beta and IL-8 were strongly up-regulated whereas IL-6 and IL-12 p35 mRNA were increased to a lesser extent and the expression of MIP-1 alpha mRNA remained constant. Finally, ELISA tests demonstrated that DCs incubated with multivalent sFc gamma RIII secreted the cytokines IL-1 beta, IL-6, IL-8, GM-CSF and IL-12 p75. Thus, while becoming internalized sFc gamma RIII could affect the capacity of DCs to present antigens and, via the induction of accessory molecules and the release of the IL-12 p75 protein, could initiate Th1 type immune response.
authors.affiliation: CJF 94-03 INSERM, Strasbourg, France.
authors.forename: H
authors.initials: H
authors.lastname: de la Salle
authors.forename: J
authors.initials: J
authors.lastname: Galon
authors.forename: H
authors.initials: H
authors.lastname: Bausinger
authors.forename: D
authors.initials: D
authors.lastname: Spehner
authors.forename: A
authors.initials: A
authors.lastname: Bohbot
authors.forename: J
authors.initials: J
authors.lastname: Cohen
authors.forename: J P
authors.initials: JP
authors.lastname: Cazenave
authors.forename: W H
authors.initials: WH
authors.lastname: Fridman
authors.forename: C
authors.initials: C
authors.lastname: Sautès
authors.forename: D
authors.initials: D
authors.lastname: Hanau
mesh_terms: D017951:Antigen Presentation; D001665:Binding Sites; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D004705:Endocytosis; D006680:HLA Antigens / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D018664:Interleukin-12 / Q000096:biosynthesis*; D007519:Isoantigens; D007959:Lymphocyte Culture Test, Mixed; D008565:Membrane Proteins; D011951:Receptors, Complement / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism*; D012995:Solubility; D018417:Th1 Cells / Q000276:immunology
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D008565:Membrane Proteins; D011951:Receptors, Complement; D017455:Receptors, IgE; D017452:Receptors, IgG; C085567:complement C3a receptor; D018664:Interleukin-12