Anhidrotic ectodermal dysplasia (EDA) protein expressed in MCF-7 cells associates with cell membrane and induces rounding.
Anhidrotic ectodermal dysplasia (EDA) is a rare X-linked recessive disorder characterized by the absence or hypoplasia of hair, teeth and sweat glands. The gene responsible for the disorder has recently been cloned. The predicted gene product is a 135 amino acid protein with no significant homology to previously known proteins. As a first step to analyze function, we have studied the subcellular localization of the EDA gene product expressed in two epithelial cell lines, COS-1 and MCF-7. Biochemical fractionation and confocal imaging analysis show that, in agreement with a single putative transmembrane domain inferred from its sequence, the EDA protein is transported to the plasma membrane. Moreover, in MCF-7 cells expression of EDA is associated with rounding and detachment of the cells. These results suggest that the EDA protein may be involved in cellular dynamics or signaling.
Ezer
S
S
Department of Medical Genetics, Haartman Institute, University of Helsinki, Finland.
Schlessinger
D
D
Srivastava
A
A
Kere
J
J
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D019556:COS Cells; D002450:Cell Communication; D048429:Cell Size; D004476:Ectodermal Dysplasia / Q000235:genetics*; D053331:Ectodysplasins; D015870:Gene Expression; D008040:Genetic Linkage; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D018613:Microscopy, Confocal / Q000379:methods; D014162:Transfection; D014960:X Chromosome
C506018:EDA protein, human; D053331:Ectodysplasins; D008565:Membrane Proteins