Consideration of the pH-dependent inhibition of dihydrofolate reductase by methotrexate.
Poisson-Boltzmann calculations were used to determine the pKa of protein functional groups in the unliganded dihydrofolate reductase enzyme, and the pKa of protein and ligand groups in methotrexate-enzyme complexes. The results reported here are in conflict with two fundamental tenets of dihydrofolate reductase inhibition by methotrexate: (1) Asp27 is not expected to be protonated near pH 6.5 in the apoenzyme as previously proposed based on fitting of empirical equations to binding data, and (2) the calculated pKa for the pteridine N1 of the inhibitor while bound to the protein is significantly lower than that estimated for this group from interpretation of NMR data (>10). In fact, the electrostatic calculations and complementary quantum chemical calculations indicate that Asp27 is likely protonated when methotrexate is bound, resulting in a neutral dipole-dipole interaction rather than a salt-bridge between the enzyme and the inhibitor. Reasons for this discrepancy with the experimental data are discussed. Furthermore, His45 and Glu17 in the Escherichia coli enzyme are proposed to be in part responsible for the pH dependence of the conformational degeneracy in the inhibitor-enzyme complex.
Cannon
W R
WR
Department of Chemistry 152 Davey Laboratory, Pennsylvania State University, University Park, PA 16802, USA.
Garrison
B J
BJ
Benkovic
S J
SJ
D010129:4-Aminobenzoic Acid / Q000737:chemistry; D019342:Acetic Acid / Q000737:chemistry; D001051:Apoenzymes / Q000737:chemistry; D001224:Aspartic Acid; D001665:Binding Sites; D004791:Enzyme Inhibitors / Q000737:chemistry*; D005493:Folic Acid Antagonists / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008727:Methotrexate / Q000494:pharmacology*; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary; D055672:Static Electricity; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry*; D013816:Thermodynamics
D001051:Apoenzymes; D004791:Enzyme Inhibitors; D005493:Folic Acid Antagonists; D008024:Ligands; D001224:Aspartic Acid; D013762:Tetrahydrofolate Dehydrogenase; D019342:Acetic Acid; D010129:4-Aminobenzoic Acid; D008727:Methotrexate