Analysis of macromolecules using nanoelectrospray ionization mass spectrometry and low-energy collision activation.
The mass spectrometric analysis of several proteins using nanoelectrospray (nanoES) with elastic collisions showed an improvement in the sensitivity over nanoES without collisional activation. We believe this effect is due to a better declusterization/ionization process. Optimization of the collision parameters can be easily performed during the long experiment time allowed using the nanoES source. Moreover, an apparent shift in the charge-state distribution is observed, with lower charged ions becoming relatively more abundant with increasing either target gas pressure or kinetic energy of the precursor ions. Higher charge-state ions might be expected to have higher collision frequencies and correspondingly lose more kinetic energy than lower charge-state ions.
Guy
P A
PA
Department of Analytical Chemistry, Glaxo Wellcome Inc., Research Triangle Park, North Carolina 27709, USA.
Anderegg
R J
RJ
D000818:Animals; D002417:Cattle; D002645:Chickens; D004529:Egg White; D006454:Hemoglobins / Q000032:analysis; D046911:Macromolecular Substances; D013058:Mass Spectrometry / Q000379:methods*; D009113:Muramidase / Q000032:analysis; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
D006454:Hemoglobins; D046911:Macromolecular Substances; D011506:Proteins; D009113:Muramidase