title: Differential regulations of matrix metalloproteinases and tissue inhibitors of metalloproteinases in dermal fibroblasts by dibutyril cyclic AMP.
abstract: The influence of dibutyril cyclic AMP (dbcAMP) on the gene expression of matrix metalloproteinases (MMP)-1, MMP-2, MMP-3, tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2 was investigated in human skin fibroblasts by northern analyses. The treatment of dbcAMP increased MMP-1 and MMP-3 mRNA levels but decreased TIMP-1 mRNA levels in time and dose dependent manners. Procollagen alpha 1 (I), MMP-2 and TIMP-2 mRNA levels were unaltered by this reagent. Our data indicate that dbcAMP potentially enhances the degradation of extracellular components of connective tissue.
authors.affiliation: Department of Dermatology, School of Medicine, Chiba University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kuroda
authors.forename: H
authors.initials: H
authors.lastname: Shinkai
mesh_terms: D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics; D017364:Collagenases / Q000235:genetics; D005109:Extracellular Matrix / Q000201:enzymology; D005347:Fibroblasts / Q000378:metabolism; D018093:Gelatinases / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D020781:Matrix Metalloproteinase 1; D020778:Matrix Metalloproteinase 2; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000235:genetics*; D011480:Protease Inhibitors / Q000378:metabolism*; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2; D019714:Tissue Inhibitor of Metalloproteinases; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D006023:Glycoproteins; D011480:Protease Inhibitors; D011506:Proteins; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D019716:Tissue Inhibitor of Metalloproteinase-2; D003994:Bucladesine; D003094:Collagen; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1