Assignment of the human gene for KBF2/RBP-Jk to chromosome 9p12-13 and 9q13 by fluorescence in situ hybridization.
The transcription factor KBF2 has been characterized as a factor that binds to the NFkB site of mouse major histocompatibility complex (MHC) class I genes and its amino acid sequence has been shwn to be identical to those of members of the recombination signal-sequence binding protein (RBP-Jk) family. Previous studies by Amakawa et al. (Genomics 17, 306-315, 1993) demonstrated that the functional gene is localized at human chromosome 3q25. However, in the present study we showed by in situ hybridization with the functional KBF2/RBPJk cosmid clone that the gene is localized at 9p12-13 and 9q13, namely, at the same loci as pseudogenes that were reported previously (Zhang et al, Jpn J Human Genet 39, 391-401, 1994).
Tang
X
X
Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Saito-Ohara
F
F
Song
J
J
Koga
C
C
Ugai
H
H
Murakami
H
H
Ikeuchi
T
T
Yokoyama
K K
KK
D000818:Animals; D002899:Chromosomes, Human, Pair 9; D003360:Cosmids; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D009687:Nuclear Proteins; D014157:Transcription Factors / Q000235:genetics
D004268:DNA-Binding Proteins; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D009687:Nuclear Proteins; C497570:RBPJ protein, human; C497571:Rbpj protein, mouse; D014157:Transcription Factors