Multiplication and haemadsorbing activity of infectious salmon anaemia virus in the established Atlantic salmon cell line.
Infectious salmon anaemia virus (ISAV), which previously had never been isolated in any of the commercially available established fish cell lines, was successfully propagated in the continuous cell line Atlantic salmon (AS). The yield of infectious ISAV increased with the incubation time of virus-inoculated cells, demonstrated by in vivo infectivity trials in groups of Atlantic salmon. Trypsin treatment of the virus was not necessary for primary infection of AS cells with salmon-grown ISAV. The infection was non-cytopathic, but it was possible to detect virus-infected cells by a haemadsorption centre assay using Atlantic salmon erythrocytes. Pleomorphic enveloped virus particles were seen by transmission electron microscopy of infected AS cells. Elongated forms were observed, but spherical particles with diameters of 90-130 nm were commonest. Growth of ISAV was inhibited by actinomycin D but not by 5-bromo-2-deoxyuridine treatment, which indicates that ISAV may be an aquatic orthomyxovirus.
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Norwegian Institute of Fisheries and Aquaculture Ltd, Tromsø. annis@fishforsk.norut.no
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D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D004912:Erythrocytes / Q000502:physiology / Q000821:virology; D006382:Hemadsorption / Q000502:physiology*; D008854:Microscopy, Electron; D009975:Orthomyxoviridae / Q000145:classification / Q000502:physiology* / Q000648:ultrastructure; D012474:Salmon; D014779:Virus Replication / Q000187:drug effects
D003609:Dactinomycin; D001973:Bromodeoxyuridine