title: P2X7 purinoceptor expression in Xenopus oocytes is not sufficient to produce a pore-forming P2Z-like phenotype.
abstract: The purinergic rP2X7 receptor expressed in a number of heterologous systems not only functions as a cation channel but also gives rise to a P2Z-like response, i.e. a reversible membrane permeabilization that allows the passage of molecules with molecular masses of > or = 300 Da. We investigated the properties of rP2X7 receptors expressed in Xenopus oocytes. In two-electrode voltage-clamp experiments, ATP or BzATP caused inward currents that were abolished or greatly diminished when NMDG+ or choline replaced Na+ as the principal external cation. In fluorescent dye experiments, BzATP application did not result in entry of the fluorophore YO-PRO-1(2+). Thus, rP2X7 expression in Xenopus oocytes does not by itself give rise to the pore-forming P2Z phenotype, suggesting that ancillary factors are involved.
authors.affiliation: Department of Physiology, University of Massachusetts Medical School, Worcester 01655-0127, USA.
authors.forename: S
authors.initials: S
authors.lastname: Petrou
authors.forename: M
authors.initials: M
authors.lastname: Ugur
authors.forename: R M
authors.initials: RM
authors.lastname: Drummond
authors.forename: J J
authors.initials: JJ
authors.lastname: Singer
authors.forename: J V
authors.initials: JV
authors.lastname: Walsh
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001583:Benzoxazoles; D002463:Cell Membrane Permeability; D004594:Electrophysiology; D005456:Fluorescent Dyes / Q000378:metabolism; D015870:Gene Expression; D007473:Ion Channels / Q000378:metabolism*; D008536:Meglumine / Q000378:metabolism; D008845:Microinjections; D009865:Oocytes; D018408:Patch-Clamp Techniques; D010641:Phenotype; D011806:Quinolinium Compounds; D018075:RNA, Complementary / Q000235:genetics; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000378:metabolism*; D058486:Receptors, Purinergic P2X7; D011994:Recombinant Proteins / Q000378:metabolism; D014981:Xenopus
chemical_list: D001583:Benzoxazoles; D005456:Fluorescent Dyes; D007473:Ion Channels; D011806:Quinolinium Compounds; D018075:RNA, Complementary; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D011994:Recombinant Proteins; C089813:YO-PRO 1; C033901:3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate; D008536:Meglumine; D000255:Adenosine Triphosphate