title: Partial purification of smooth muscle cell derived growth factor.
abstract: The serum free medium conditioned by cultured rabbit aortic smooth muscle cells was partially purified using ultrafiltration and heparin affinity chromatography. Incorporation of [3H]-thymidine (3H-TdR) into cell DNA was used to measure the mitogenic activity of the fractions from chromatography for NIH 3T3 fibroblasts. The molecular weight and the iso-electric point of these fractions were determined by NaDodSO4-polyacrylamide gel electrophoresis (SDS-PAGE) and iso-electric focusing, respectively. The results showed that the protein eluted in 1.0-1.6 mol/L NaCl from the heparin-Sepharose was mitogenic for 3T3 cells, and this protein had a molecular weight of 22.8-26.7 ku and an iso-electric point of about 4.6. The fact that the above-mentioned biochemical properties differed from that of PDGF, IGF and FGF suggests that this mitogenic protein may be a separate growth factor.
authors.affiliation: Department of Pathology, Tongji Medical University, Wuhan.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: Z
authors.initials: Z
authors.lastname: Deng
authors.forename: Z
authors.initials: Z
authors.lastname: Qu
mesh_terms: D016475:3T3 Cells / Q000166:cytology; D000818:Animals; D001011:Aorta; D006133:Growth Substances / Q000096:biosynthesis / Q000302:isolation & purification*; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D011817:Rabbits; D017540:Tunica Media / Q000166:cytology
chemical_list: D006133:Growth Substances