title: FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes.
abstract: The ability to place a series of gene constructs at a specific site in the genome opens new possibilities for the experimental examination of gene expression and chromosomal position effects. We report that the FLP- FRT site-specific recombination system of the yeast 2mu plasmid can be used to integrate DNA at a chromosomal FRT target site in Drosophila. The technique we used was to first integrate an FRT- flanked gene by standard P element-mediated transformation. FLP was then used to excise the FRT- flanked donor DNA and screen for FLP-mediated re-integration at an FRT target at a different chromosome location. Such events were recovered from up to 5% of the crosses used to screen for mobilization and are easily detectable by altered linkage of a white reporter gene or by the generation of a white + gene upon integration.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, UT 84112, USA. golic@bioscience.utah.edu
authors.forename: M M
authors.initials: MM
authors.lastname: Golic
authors.forename: Y S
authors.initials: YS
authors.lastname: Rong
authors.forename: R B
authors.initials: RB
authors.lastname: Petersen
authors.forename: S L
authors.initials: SL
authors.lastname: Lindquist
authors.forename: K G
authors.initials: KG
authors.lastname: Golic
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics*; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D005786:Gene Expression Regulation; D018390:Gene Targeting; D017344:Genes, Insect; D011995:Recombination, Genetic
chemical_list: D004247:DNA; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase