Regulation by tetracycline of gene expression in Saccharomyces cerevisiae.
A convenient system for the control of gene expression in Saccharomyces cerevisiae was developed. Tetracycline-responsive promoters were constructed by fusing the tetracycline operator (tetO) to the S. cerevisiae HOP1 promoter. When fused to the tetracycline repressor (tetR), trans-activation domains of both GAL4 and HAP4 were capable of promoting transcription from the tetO-HOP1 chimeric promoter, but the tetR-HAP4 fusion activator was the more efficient transcriptional activator. Addition of tetracycline nearly completely repressed activator-dependent transcription from the tetO-HOP1 promoter. Moreover, tetracycline-dependent repression of YEF3, CDC28 and RAM2 expression impaired cell growth. Thus, this system is useful for the elucidation of gene function in S. cerevisiae.
Nagahashi
S
S
Department of Mycology, Nippon Roche Research Center, Kanagawa, Japan.
Nakayama
H
H
Hamada
K
K
Yang
H
H
Arisawa
M
M
Kitada
K
K
D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005818:Genetic Engineering / Q000379:methods*; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013752:Tetracycline / Q000494:pharmacology*
D012097:Repressor Proteins; C034811:tetracycline resistance-encoding transposon repressor protein; D013752:Tetracycline