title: Inhibition of reduced DP18-maltodextrin and amylose hydrolysis by acarbose: kinetic studies.
abstract: Kinetics of inhibition of porcine pancreatic alpha-amylase by acarbose were performed using maltodextrin and amylose as substrates. Similar Lineweaver-Burk primary plots were obtained. Two mixed non-competitive models are proposed. X-ray crystallographic data (Qian, M., Buisson, G., Duée, E., Haser, R. and Payan, F. Biochemistry, 1994; 33: 6284-6294) are in support of the mixed non-competitive inhibition model which involves abortive complexes. Secondary plots are different indicating that in the maltodextrin hydrolysis, one molecule of acarbose is bound per amylase molecule, while using amylose as substrate two molecules of acarbose are bound. These two kinetically determined binding sites might correspond to the two surface sites shown by X-ray crystallography (Qian, M., Haser, R. and Payan, F. Protein Science 1995; 4: 747-755).
authors.affiliation: Laboratoire de Biochimie et Biologie de la Nutrition URA-CNRS 1820, Faculté des Sciences et Techniques de St Jérôme, Université d'Aix-Marseille III, France.
authors.forename: M
authors.initials: M
authors.lastname: Alkazaz
authors.forename: V
authors.initials: V
authors.lastname: Desseaux
authors.forename: E
authors.initials: E
authors.lastname: Prodanov
authors.forename: G
authors.initials: G
authors.lastname: Marchis-Mouren
authors.forename: M
authors.initials: M
authors.lastname: Santimone
mesh_terms: D020909:Acarbose; D000688:Amylose / Q000378:metabolism*; D001665:Binding Sites; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D008956:Models, Chemical; D011134:Polysaccharides / Q000378:metabolism*; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D011134:Polysaccharides; D014312:Trisaccharides; C008315:maltodextrin; D000688:Amylose; D000516:alpha-Amylases; D020909:Acarbose