Rat kidney thromboxane synthase: cDNA cloning and gene expression regulation in hydronephrotic kidney.
We isolated a rat homolog of thromboxane (TX) synthase cDNA (-1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver, and uterus; moderately in kidney. TX synthase mRNA expression was up-regulated in hydronephrotic kidney made by ureter ligation. In conclusion, we have revealed the structure of rat kidney TX synthase. Up-regulation of renal TX synthase, which may cause stimulation of TX synthesis, is possibly implicated in the tissue injury in hydronephrotic kidney.
Tsutsumi
E
E
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Takeuchi
K
K
Abe
T
T
Takahashi
N
N
Kato
T
T
Taniyama
Y
Y
Ikeda
Y
Y
Ito
S
S
Abe
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006869:Hydronephrosis / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology*; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013930:Thromboxane-A Synthase / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D013930:Thromboxane-A Synthase