[Fast DNA amplification in small ultrathin-wall microplates].
A new method was developed for fast DNA amplification by polymerase chain reaction in tiny ultrathin microplates formed directly on the thermocycler's thermoblock. The microplates are made from thin (40-60 microns) polypropylene film by the thermal vacuum-formation method. Due to the effective heat transfer to 10-15 microliters samples and a high velocity of heating and cooling of the thermoblock (up to 7 degrees C/s), the total duration of the DNA amplification (30 cycles) is only 15-30 min.
Tret'iakov
A N
AN
Pantina
R A
RA
Kaboev
O K
OK
D004279:DNA, Viral; D005784:Gene Amplification; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*
D004279:DNA, Viral