Participation of deoxyribonucleic acid polymerase alpha in amplification of ribosomal deoxyribonucleic acid in Xenopus laevis.
Aphidicolin, a known inhibitor of eucaryotic deoxyribonucleic acid (DNA) polymerase alpha, efficiently inhibited amplification of ribosomal DNA during oogenesis in Xenopus laevis. DNA polymerase alpha, but not DNA polymerase gamma, as isolated from ovaries, was sensitive to aphidicolin. DNA polymerase beta was not detectable in Xenopus ovary extracts. Therefore, DNA polymerase alpha plays a major role in ribosomal ribonucleic acid gene amplification.
Zimmermann
W
W
Department of Cell Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110, USA.
Weissbach
A
A
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005784:Gene Amplification / Q000187:drug effects*; D009866:Oogenesis / Q000235:genetics; D014982:Xenopus laevis
D004275:DNA, Ribosomal; D004791:Enzyme Inhibitors; D016590:Aphidicolin; D004257:DNA Polymerase II