Barley elongation factor 1 alpha: genomic organization, DNA sequence, and phylogenetic implications.
A full length cDNA clone encoding the 447 amino acid long barley (Hordeum vulgare cv. Bomi) endosperm elongation factor 1 alpha (eF-1 alpha) was isolated by a differential screening procedure. RFLP mapping of eF-1 alpha showed that the barley genome contains a small eF-1 alpha gene family of 4 copies, with 1 copy of the gene being located on each of chromosomes 2, 4, 6, and 7. Analysis of barley endosperm total proteins by Western blot with antibodies directed towards wheat eF-1 alpha and the sea urchin 51 kDa proteins gave a single band of the expected molecular weight. Amino acid sequence comparison with other plant eF-1 alpha sequences showed that the isolated barley endosperm eF-1 alpha is more similar to the published wheat eF-1 alpha sequence than to eF-1 alpha sequences previously published for the barley cultivars Igri and Dicktoo. The phylogenetic analysis suggests that the barley eF-1 alpha gene family can be divided into two subfamilies and that two ancestral genes existed before the divergence of monocotyledonous and dicotyledonous plants.
Nielsen
P S
PS
Department of Biotechnological Sciences, Agricultural University of Norway, Aas, Norway. psni@biobase.dk
Kleinhofs
A
A
Olsen
O A
OA
D000595:Amino Acid Sequence; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics*; D019143:Evolution, Molecular; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*
D018744:DNA, Plant; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010940:Plant Proteins