Human endometriosis-derived permanent cell line (FbEM-1): establishment and characterization.
A human epithelial-like cell line derived from peritoneal implants from a patient with gonadotrophin-releasing hormone (GnRH) agonist-resistant endometriosis graded as stage IVd according to the American Fertility Society classification was established in vitro. This cell line, designated FbEM-1, exhibited an epithelial-like morphology, grew in suspension and was immunoreactive for cytokeratins 8, 18, 19, vimentin and human leukocyte class I antigens. The cultured cells were negative for various haematopoietic cell markers, including the lymphoid cell antigens CD3, CD20 and CD45, von Willebrandt factor, carcinoembryonic antigen and the carcinoma antigen-125 (CA-125). In addition, the FbEM-1 cells were found to be moderately positive for periodic acid Schiff's (PAS) solution but were negative for alpha-naphthyl acetate esterase, peroxidase and chloroacetate esterase activities. Using specific antibodies against the progesterone, androgen and oestrogen receptors, approximately 40% and 5-10% of the cells immunostained for progesterone and androgen receptors respectively, while oestrogen receptors were not detected. On cytogenetic analysis using R-banding, these cells showed numerous chromosomal aberrations, including loss of one chromosome X, 4q+, 5q+, trisomy 7,8 and 10 and tetrasomy of chromosomes 17, 18, 19 and 20. These data show that the continuously growing FbEM-1 cell line established from endometriotic implants may be useful in achieving better understanding of the histogenesis of endometriosis.
Bouquet de Jolinière
J
J
Service de Gynécologie-Obstétrique, Hôpital Beaujon, Clichy, France.
Validire
P
P
Canis
M
M
Doussau
M
M
Levardon
M
M
Gogusev
J
J
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D017690:Cell Transplantation; D002869:Chromosome Aberrations; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D007633:Keratins / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D010503:Periodic Acid-Schiff Reaction; D011944:Receptors, Androgen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D000954:Antigens, Surface; D015395:Histocompatibility Antigens Class I; D011944:Receptors, Androgen; D011980:Receptors, Progesterone; D014746:Vimentin; D007633:Keratins