Glutamine accelerates interleukin-6 production by rat peritoneal macrophages in culture.
The effect of glutamine on the production of interleukin-6 (IL-6) was studied in rat peritoneal macrophages in culture. A maximal production of IL-6 was measured at 4 h in lipopolysaccharide (LPS)-stimulated macrophages, and addition of glutamine (5 mM) anticipated this increase by 1 h without any increase in the IL-6 mRNA level. The effect of glutamine required the presence of LPS. Thus, glutamine accelerates IL-6 production from the pre-existing mRNA. The effect of glutamine was not mediated by cell swelling since culture of macrophages in hypoosmotic condition decreased the production of IL-6 in the culture medium with a corresponding decrease in the IL-6 mRNA level.
Yassad
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Groupe de Biochimie et Physiopathologie Digestive et Nutritionnelle, Institut Fédératif de Recherches Multidisciplinaires sur les Peptides No. 23, UFR Médecine-Pharmacie de Rouen, Saint-Etienne-du-Rouvray, France.
Lavoinne
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Bion
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Daveau
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Husson
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D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005973:Glutamine / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D005973:Glutamine