title: Comparison of cytogenetics, cytokine secretion, and oncogene expression in primary cultures of renal carcinoma cells.
abstract: We compared the cytogenetic pattern of 20 different primary tumor cell cultures (PTCC) of renal cell carcinoma (RCC) to their cytokine secretion and oncogene expression. High secretion of IL-6 (gene locus on chromosome 7p21-p14) was correlated with the gain of an additional chromosome 7. Structural changes involving chromosome 5q22, the site of the GM-CSF gene, were matched with the high secretion of GM-CSF in PTCC. No such association was found for beta 2-microglobulin, TGF-beta 1, TNF-alpha, IL-8, and oncogenes, such as c-fos, c-myc, and pan-ras. Our approach may be useful in simultaneously analyzing several factors contributing to tumor progression and may contribute to understanding the multistep development of RCC.
authors.affiliation: Abteilung Innere Medizin I, Hämatologie-Onkologie, Klinikum der Albert-Ludwigs-Universität Freiburg, Germany. MichaLahn@aol.com
authors.forename: M
authors.initials: M
authors.lastname: Lahn
authors.forename: R
authors.initials: R
authors.lastname: Kunzmann
authors.forename: G
authors.initials: G
authors.lastname: Köhler
authors.forename: D N
authors.initials: DN
authors.lastname: Iklé
authors.forename: I
authors.initials: I
authors.lastname: Hentrich
authors.forename: H
authors.initials: H
authors.lastname: Jesuiter
authors.forename: P
authors.initials: P
authors.lastname: Kulmburg
authors.forename: H
authors.initials: H
authors.lastname: Veelken
authors.forename: A
authors.initials: A
authors.lastname: Mackensen
authors.forename: F
authors.initials: F
authors.lastname: Rosenthal
authors.forename: A
authors.initials: A
authors.lastname: Lindemann
mesh_terms: D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004815:Epidermal Growth Factor / Q000096:biosynthesis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D011519:Proto-Oncogenes / Q000235:genetics; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; D007378:Interleukins; D016211:Transforming Growth Factor alpha; D014409:Tumor Necrosis Factor-alpha; D016159:Tumor Suppressor Protein p53; D018799:Intercellular Adhesion Molecule-1; D004815:Epidermal Growth Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor