[Gene expression of HGF and its receptor in experimental hepatomas].
OBJECTIVE
To understand the regularity of changes of gene expression about HGF and its receptor (HGFR) in experimental hepatomas model of Wistar rats and the relationship between this change and tumor biological behaviour.


METHODS
The gene expression of HGF and HGFR in the canceration course of rats was determined by using experimental hepatomas model of Wistar rats established by diethylnitrosamine (DENA), with digoxigenin-labled probe.


RESULTS
The positive expression of HGF and HGFR was found in rats liver tissues, but it was different in distinct stages. The positive expression of HGF was the highest in precirrhosis stage, and next in the cirrhosis stage. The canceration stage was the same as in the normal group. The positive expression of HGFR was gradually increased following the canceration course and the canceration stage was the highest, but we didn't find the expression of HGFR in normal liver tissues.


CONCLUSIONS
There are close relations between the gene expression of HGF and HGFR and evolution course during the canceration of rats liver. HGF and HGFR systems play an important role in regulating tumor growth and metastasis.
Luo
Y
Y
East Hospital of Hepatobiliary Surgery, Second Military Medical University, Shanghai.
Wu
M
M
Chen
H
H
D000818:Animals; D004052:Diethylnitrosamine; D015972:Gene Expression Regulation, Neoplastic; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019859:Proto-Oncogene Proteins c-met; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*
D011518:Proto-Oncogene Proteins; D004052:Diethylnitrosamine; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met; D020794:Receptor Protein-Tyrosine Kinases