Electromagnetic field exposure induces rapid, transitory heat shock factor activation in human cells.
Stimulation of human promyelocytic HL60 cells by a 60Hz magnetic field at normal growth temperatures results in heat shock factor 1 activation and heat shock element binding, a sequence of events that mediates the stress-induced transcription of the stress gene HSP70 and increased synthesis of the stress response protein hsp70kD. Thus, the events mediating the electromagnetic field-stimulated stress response appear to be similar to those reported for other physiological stresses (e.g., hyperthermia, heavy metals, oxidative stress) and could well be the general mechanism of interaction of electromagnetic fields with cells.
Lin
H
H
Department of Pathology, Columbia University Health Sciences, New York, New York 10032, USA.
Opler
M
M
Head
M
M
Blank
M
M
Goodman
R
R
D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D018922:HL-60 Cells / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000502:physiology; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological / Q000503:physiopathology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology
D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D014157:Transcription Factors; C070389:HSF2 protein, human