title: A new method of synthesis of fluorescently labelled oligonucleotides and their application in DNA sequencing.
abstract: A new approach to the chemical synthesis of oligodeoxynucleotides bearing reporter functional groups at base residues of 3'-end nucleosides is reported. Applications of the 3'-end fluorescently labelled primers for automated DNA sequencing are shown.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12, PL-61704 Poznan, Poland. markw@ibch.posnon.pl
authors.forename: W T
authors.initials: WT
authors.lastname: Markiewicz
authors.forename: G
authors.initials: G
authors.lastname: Gröger
authors.forename: R
authors.initials: R
authors.lastname: Rösch
authors.forename: A
authors.initials: A
authors.lastname: Zebrowska
authors.forename: M
authors.initials: M
authors.lastname: Markiewicz
authors.forename: M
authors.initials: M
authors.lastname: Klotz
authors.forename: M
authors.initials: M
authors.lastname: Hinz
authors.forename: P
authors.initials: P
authors.lastname: Godzina
authors.forename: H
authors.initials: H
authors.lastname: Seliger
mesh_terms: D005456:Fluorescent Dyes; D015336:Molecular Probe Techniques; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides