The survival rate of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Bacteroides forsythus following 4 randomized treatment modalities.
The overall goal of this clinical study was to determine the short-term anti-infective effects of four randomized treatment modalities on Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), and Bacteroides forsythus (Bf) and determine the effects of bacterial survival on treatment outcomes in patients with adult periodontitis. Twelve adult patients requiring therapy for moderate periodontitis were selected for this study. All patients had at least one tooth in each quadrant that had an inflamed pocket of probing depth > or =5 mm with probing attachment loss that harbored at least one of the following three periodontal pathogens: Aa, Pg, or Bf. The number of target organisms per site was determined pre-operatively, at 1 week, and 1 month and 3 months postoperatively utilizing DNA probes. One quadrant in each patient was randomly assigned to each one of the following four treatment groups: 1) scaling and root planing (SRP group); 2) pocket reduction through osseous surgery and apically-positioned flap (OS group); 3) modified Widman flap (MWF group); and 4) modified Widman flap and topical application of saturated citric acid at pH 1 for 3 minutes (CA group). The 4 treatment modalities were performed in one appointment. No postoperative antibiotics were used. Patients were instructed to supplement their daily oral hygiene with chlorohexidine oral rinse during the study. The results of this investigation indicated that: 1) none of the treatment modalities was effective in eliminating the target species; 2) the incidence of infected sites for all groups was 100% preoperatively; 62.5%, 33.3%, and 31.3% at 1 week, and 1 and 3 months postoperatively, respectively; 3) these infected sites lost 1.1 +/- 0.4 mm of probing attachment compared to gain of 0.0 +/- 0.3 mm for uninfected sites; 4) the infected sites had higher plaque and bleeding on probing 0.9 +/- 0.3, 73 +/- 12%, respectively, compared to 0.3 +/- 0.1 and 30 +/- 8% for the uninfected sites; and 5) no statistically significant differences were detected among the infected sites in regard to gingival index (1.0 +/- 0.2 vs. 0.8 +/- 0.1) or probing depth (3.5 +/- 0.4 vs. 3.0 +/- 0.1 mm). These results indicate that bacterial survival negatively affects the short-term clinical outcomes of non-surgical and surgical periodontal therapy.
Shiloah
J
J
Department of Periodontology, College of Dentistry, University of Tennessee, Memphis 38163, USA.
Patters
M R
MR
Dean
J W
JW
Bland
P
P
Toledo
G
G
D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000502:physiology*; D000543:Alveoloplasty; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D001439:Bacteroides / Q000235:genetics / Q000502:physiology*; D002614:Chelating Agents / Q000008:administration & dosage / Q000627:therapeutic use; D002710:Chlorhexidine / Q000627:therapeutic use; D019343:Citric Acid / Q000008:administration & dosage / Q000627:therapeutic use; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology; D012534:Dental Scaling; D005260:Female; D005500:Follow-Up Studies; D005884:Gingival Hemorrhage / Q000382:microbiology; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D009067:Mouthwashes; D009910:Oral Hygiene; D017622:Periodontal Attachment Loss / Q000382:microbiology / Q000601:surgery / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000382:microbiology / Q000601:surgery / Q000628:therapy; D010518:Periodontitis / Q000382:microbiology / Q000601:surgery / Q000628:therapy*; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000502:physiology*; D016745:Root Planing; D013524:Surgical Flaps / Q000379:methods; D016896:Treatment Outcome
D000891:Anti-Infective Agents, Local; D002614:Chelating Agents; D015342:DNA Probes; D004269:DNA, Bacterial; D009067:Mouthwashes; D019343:Citric Acid; D002710:Chlorhexidine