title: Phosphorimetric determination of indomethacin in pharmaceutical formulations.
abstract: A simple, rapid and sensitive method for the determination of indomethacin was developed based on solid-surface room-temperature phosphorimetry. Phosphorescence emission was induced by the indomethacin hydrolysis reaction in a basic medium. Chromatography paper treated for background reduction was employed as a solid substrate. The use of four heavy-atom salts and sodium dodecyl sulfate was optimized to estimate an absolute limit of detection at the subnanogram level. A calibration curve with a linear dynamic range extending over two orders of magnitude (3.0 x 10(-7)-1.0 x 10(-4) mol dm-3) was obtained. The recovery of the method (98.4 +/- 6.8%) was tested using commercial formulations containing indomethacin as the only anti-inflammatory agent.
authors.affiliation: Department of Chemistry, North Dakota State University, Fargo 58105-5516, USA.
authors.forename: A F
authors.initials: AF
authors.lastname: Arruda
authors.forename: A D
authors.initials: AD
authors.lastname: Campiglia
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002845:Chromatography; D007213:Indomethacin / Q000032:analysis* / Q000737:chemistry; D008163:Luminescent Measurements; D004364:Pharmaceutical Preparations / Q000737:chemistry*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004364:Pharmaceutical Preparations; D007213:Indomethacin