Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of the Bacillus subtilis genome reveal genes for a new two-component system, three spore germination proteins, an iron uptake system and a general stress response protein.
In the framework of the international project aimed at sequencing the Bacillus subtilis (Bs) genome, a 35.7-kb chromosome segment around the pel locus has been cloned and sequenced. This region (35,745 bp; 70 degrees-73 degrees of the genetic map) contains two partial and 38 complete orfs. A homology search for the products deduced from the 39 orfs revealed that 26 of them exhibit significant similarity to known proteins, e.g. germination proteins, sodium-alanine symporter, PTS system, methionine amino peptidase, 2-oxoglutarate/malate translocater, pectate lyase, general stress response protein, RNA helicase, iron uptake and two-component systems.
Yamamoto
H
H
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Uchiyama
S
S
Nugroho
F A
FA
Sekiguchi
J
J
D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D002951:Citrates / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D011133:Polysaccharide-Lyases / Q000235:genetics; D013171:Spores, Bacterial / Q000235:genetics
D001426:Bacterial Proteins; D002951:Citrates; D004269:DNA, Bacterial; D007501:Iron; D011133:Polysaccharide-Lyases; C023911:pectate lyase