Characterization of a pollination-related cDNA from Phalaenopsis encoding a protein which is homologous to human peroxisomal acyl-CoA oxidase.
The first putative plant acyl-CoA oxidase cDNA has been isolated from a Phalaenopsis cDNA library constructed by poly(A)+ RNA extracted from petals 1 day after pollination. This cDNA, pOACO31, contains a 2100-bp open reading frame which encodes a polypeptide named PACO1 of 699 amino acids. The predicted isoelectric point of PACO1 is 8.74 and the molecular weight is 78,032 Da, similar to that of a monomer of predicted plant acyl-CoA oxidase. Southern blot analysis indicated that this gene occurs in one copy or a low number of copies per haploid genome. When compared with sequences in databases, PACO1 revealed significant similarity only to peroxisomal acyl-CoA oxidase particularly within 13 conserved regions and a putative FMN-binding site.
Do
Y Y
YY
Department of Horticulture, National Taiwan University, Taipei.
Huang
P L
PL
D042962:Acyl-CoA Oxidase; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D017343:Genes, Plant; D006801:Humans; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D018521:Plant Physiological Phenomena; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011058:Pollen; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015003:Yeasts / Q000201:enzymology / Q000235:genetics
D018076:DNA, Complementary; D010088:Oxidoreductases; C107176:peroxisomal acyl-CoA oxidase; D042962:Acyl-CoA Oxidase