Amplification of Bcl I region of the factor VIII gene by PCR.
A study was initiated to amplify by polymerase chain reaction (PCR), a short factor VIII gene fragment containing the Bcl I restriction site from hemophilia patients using published primer sequences. Preliminary findings indicated that the resulting fragment is 142 bp long. This fragment, when digested with Bcl I restriction enzyme produced two fragments, 99 bp and 43 bp in length. Polymorphism in the Bcl I region can be used to detect carrier state in the family members of the hemophiliacs.
Ishak
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Hematology Division, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
Khim
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LC
D005169:Factor VIII / Q000235:genetics*; D005820:Genetic Testing; D006467:Hemophilia A / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D015183:Restriction Mapping
D005169:Factor VIII