title: In vitro mitochondrial test to assess haemodialyser biocompatibility.
abstract: BACKGROUND
  This paper describes an in vitro mitochondrial test to assess the biocompatibility of haemodialysers.
  
  
  METHODS
  We tested on isolated liver mitochondria the effect of solutions obtained by an aqueous rinse of different haemodialysers (cuprophane, cellulose acetate, Hemophan, polyacrylonitrile, polymethylmethacrylate, polysulphone, polyamide). Moreover, to determine the penetration into the cell and the cytotoxicity of these solutions from haemodialysers, we examined the effect of rinse solutions on HT29-D4 cells.
  
  
  RESULTS
  Our results showed that rinse solutions from haemodialysers decrease the mitochondrial ATP synthesis. Cuprophane has the most marked effect, and the synthetic membranes exhibited only mild effects. Rinse solutions penetrated the cell and were cytotoxic by acting on mitochondria in the cell. In this respect, cellulosic membranes were the most toxic.
  
  
  CONCLUSIONS
  Taken together our findings lead to a classification of haemodialyser membranes which is identical to one based on criteria such as activation of complement (cuprophane > other cellulosics > synthetics). Moreover isolated mitochondria make it possible to differentiate among the synthetic membranes. Isolated mitochondria thus appear to be a good in vitro test to assess the biocompatibility of haemodialysers.
authors.affiliation: Laboratoire de Biophysique, Faculté de Pharmacie, Marseille, France.
authors.forename: L J
authors.initials: LJ
authors.lastname: Tabouy
authors.forename: A M
authors.initials: AM
authors.lastname: Chauvet-Monges
authors.forename: P J
authors.initials: PJ
authors.lastname: Brunet
authors.forename: D L
authors.initials: DL
authors.lastname: Braguer
authors.forename: P A
authors.initials: PA
authors.lastname: García
authors.forename: Y F
authors.initials: YF
authors.lastname: Berland
authors.forename: A D
authors.initials: AD
authors.lastname: Crevat
mesh_terms: D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D008297:Male; D008567:Membranes, Artificial; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D006435:Renal Dialysis / Q000295:instrumentation*; D012996:Solutions / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D012996:Solutions; D000255:Adenosine Triphosphate