title: [Effect of TRA-system of plasmids RP4 and R68.45 on pseudomonas mallei virulence].
abstract: In Pseudomonas mallei, spontaneous mutants (Tra- mutants) of the plasmids RP4 and R68.45, losing the ability to transfer at conjugation are formed. The plasmids RP4 and R68.45 with Tra(+)-phenotype caused a decrease in P. mallei virulence for laboratory animals. At the same time, Tra- mutants of these plasmids do not affect P. mallei virulence. The insertion of DNA fragment of about 1900 bp into the plasmid transfer gene regions (tra-2-tra-3) gave rise to RP4 and R68.45 tra mutations detectable on examination.
authors.affiliation: State Scientific Center of Applied Microbiology, Obolensk.
authors.forename: V V
authors.initials: VV
authors.lastname: Verevkin
authors.forename: N V
authors.initials: NV
authors.lastname: Volozhantsev
authors.forename: V P
authors.initials: VP
authors.lastname: Miakinina
authors.forename: E A
authors.initials: EA
authors.lastname: Svetoch
mesh_terms: D000818:Animals; D016957:Burkholderia pseudomallei / Q000235:genetics* / Q000472:pathogenicity*; D003227:Conjugation, Genetic; D006224:Cricetinae; D004269:DNA, Bacterial / Q000235:genetics; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D008554:Melioidosis / Q000517:prevention & control; D009154:Mutation; D010641:Phenotype; D011815:R Factors / Q000235:genetics*; D014774:Virulence / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial