title: Guanosine tetra- and pentaphosphate promote accumulation of inorganic polyphosphate in Escherichia coli.
abstract: High levels of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), generated in response to amino acid starvation in Escherichia coli, lead to massive accumulations of inorganic polyphosphate (polyP). Inasmuch as the activities of the principal enzymes that synthesize and degrade polyP fluctuate only slightly, the polyP accumulation can be attributed to a singular and profound inhibition by pppGpp and/or ppGpp of the hydrolytic breakdown of polyP by exopolyphosphatase, thereby blocking the dynamic turnover of polyP. The Ki values of 10 microM for pppGpp and 200 microM for ppGpp are far below the concentrations of these nucleotides in nutritionally stressed cells. In the complex metabolic network of pppGpp and ppGpp, the greater inhibitory effect of pppGpp (compared with ppGpp) leading to the accumulation of polyP, may have some significance in the relative roles played by these regulatory compounds.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5307, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kuroda
authors.forename: H
authors.initials: H
authors.lastname: Murphy
authors.forename: M
authors.initials: M
authors.lastname: Cashel
authors.forename: A
authors.initials: A
authors.lastname: Kornberg
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D006158:Guanosine Pentaphosphate / Q000378:metabolism*; D006159:Guanosine Tetraphosphate / Q000378:metabolism*; D007700:Kinetics; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000378:metabolism*; D011122:Polyphosphates / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism
chemical_list: D011122:Polyphosphates; D006159:Guanosine Tetraphosphate; D006158:Guanosine Pentaphosphate; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase; D017766:Acid Anhydride Hydrolases; D011755:Pyrophosphatases; C020137:exopolyphosphatase; C082499:guanosine-5'-triphosphate, 3'-diphosphate pyrophosphatase