Engineering metal-binding sites in proteins.
Metal-binding sites have been engineered into both de novo designed and naturally occurring proteins. Although the redesign of existing metal-binding sites in naturally occurring proteins still offers the most promise for a successful design, the more challenging goal of engineering metal-binding sites in de novo designed proteins and peptides is being achieved with increasing frequency. Creating new metal-binding sites in naturally occurring proteins combines the strength of both approaches. Currently, all three approaches are being used effectively in elucidating the structure and function of naturally occurring metalloproteins.
Lu
Y
Y
Department of Chemistry, University of Illinois at Urbana-Champaign 61801, USA. yi-lu@uiuc.edu
Valentine
J S
JS
D001665:Binding Sites; D008667:Metalloproteins / Q000737:chemistry; D008670:Metals / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D008667:Metalloproteins; D008670:Metals; D011506:Proteins