title: Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus.
abstract: The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed.
authors.affiliation: Department of Virology, Agricultural University Wageningen, The Netherlands.
authors.forename: D T
authors.initials: DT
authors.lastname: Kasteel
authors.forename: N N
authors.initials: NN
authors.lastname: van der Wel
authors.forename: K A
authors.initials: KA
authors.lastname: Jansen
authors.forename: R W
authors.initials: RW
authors.lastname: Goldbach
authors.forename: J W
authors.initials: JW
authors.lastname: van Lent
mesh_terms: D019178:Alfamovirus / Q000502:physiology* / Q000648:ultrastructure; D017795:Bromovirus / Q000502:physiology* / Q000648:ultrastructure; D007887:Fabaceae / Q000648:ultrastructure / Q000821:virology; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D053830:Plant Viral Movement Proteins; D010946:Plants, Medicinal; D011523:Protoplasts / Q000648:ultrastructure / Q000821:virology; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D053830:Plant Viral Movement Proteins; D014764:Viral Proteins