Zinc attenuation of GDP binding to brown adipocytes mitochondria in genetically obese (ob/ob) mice.
In this study, we investigate the in vitro effect of zinc addition on guanosine diphosphate (GDP) binding to mitochondria in brown adipocytes of genetically obese (ob/ob) mice. Interscapular brown adipocytes of male mice (obese; lean) at 4 and 12 wk of age were incubated with 0, 50, 100, or 200 microM zinc sulfate. Mitochondria were then isolated and their GDP binding capacities were measured. The GDP-binding capacities of ob/ob mice were lower than lean mice, with or without zinc addition, in both age groups (p < 0.05). Zinc addition did not have any significant effect on GDP binding in lean mice. GDP binding decreased with increasing zinc addition in ob/ob mice, and this attenuation was more predominant in 12-wk old ob/ob mice. Moreover, we found that high magnesium addition (5 mM) increased GDP binding in lean mice, but this effect was not significant in ob/ob mice. This study reveals that brown adipose tissue thermogenesis in ob/ob mice could be greatly attenuated by zinc addition, suggesting that zinc may play a regulatory role in obesity.
Chen
M D
MD
Graduate Institute of Biology, Tunghai University, Taichung, Taiwan, ROC.
Lin
P Y
PY
Chen
P S
PS
Cheng
V
V
Lin
W H
WH
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002001:Adipose Tissue, Brown / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D000818:Animals; D001665:Binding Sites; D001833:Body Temperature Regulation; D006153:Guanosine Diphosphate / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology*
D006153:Guanosine Diphosphate; D008274:Magnesium; D015032:Zinc