Induction and duration of long-term potentiation in the hippocampus of the freely moving mouse.
We describe a simple method, using readily available minaturised components, for inducing and monitoring long-term potentiation (LTP) at perforant path-granule cell synapses in the dentate gyrus of the freely moving mouse. Tetanic stimulation induced LTP of the field EPSP and the population spike which persisted for more than 24 h but was not present 10 days after the tetanus. The potentiation of the population spike was proportionately greater than the potentiation of the EPSP (E-S potentiation). Induction of LTP was blocked by intraperitoneal injections of the N-methyl-D-aspartate (NMDA) receptor antagonist, 3-((RS)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP).
Davis
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S
Laboratoire de Neurobiologie de l'Apprentissage et de la Mémoire, CNRS, URA 1491, Université Paris Sud, Orsay, France. Sabrina.Davis@ibaic.u-psud.Fr
Bliss
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Dutrieux
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Laroche
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Errington
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ML
D000818:Animals; D018891:Dentate Gyrus / Q000502:physiology*; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D017774:Long-Term Potentiation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009068:Movement / Q000502:physiology*; D010879:Piperazines / Q000494:pharmacology; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors
D018691:Excitatory Amino Acid Antagonists; D010879:Piperazines; D016194:Receptors, N-Methyl-D-Aspartate; C065286:SDZ EAA 494