title: Rapid detection of mycobacteria in clinical specimens by using the automated BACTEC 9000 MB system and comparison with radiometric and solid-culture systems.
abstract: Recovery rates of acid-fast bacilli (AFB) and the mean time to their detection from clinical specimens were determined by using the fluorescent BACTEC 9000 MB system. Data were compared to those assessed by the radiometric BACTEC 460 system and by cultivation on solid media. A total of 3,095 specimens were processed with N-acetyl-L-cysteine-NaOH by two laboratories. The contamination rates for the BACTEC 9000 MB system were 6.8% (center 1) and 9.8% (center 2). A total of 451 mycobacterial isolates were detected (Mycobacterium tuberculosis complex, n = 296; nontuberculous mycobacteria [NTM], n = 155). These isolates originated from 94 (20.8%) smear-positive and 357 (79.2%) smear-negative specimens. The BACTEC 9000 MB system was significantly better than solid media (P < 0.05) in detecting AFB, but it was less efficient than the radiometric system (P < 0.01). The BACTEC 9000 MB system plus solid media (combination A) recovered 393 (87.1%) of the isolates, while the BACTEC 460 system plus solid media (combination B) detected 430 (95.3%) of all AFB isolates. Between combination A and B there was no statistically significant difference for the detection of isolates from smear-positive specimens (P > 0.05), in contrast to the recovery of AFB from smear-negative specimens for M. tuberculosis complex, P < 0.05; for NTM, P < 0.01). The mean time to detection of M. tuberculosis complex was 12.2 days for smear-positive specimens and 18.1 days for smear-negative specimens with the BACTEC 9000 MB system; 9.3 and 15.6 days, respectively, with the BACTEC 460 system; and 21.2 and 28.4 days, respectively, with solid media. For NTM, the average detection times were 15.1, 17.3, and 31.3 days by the three methods, respectively. In conclusion, the BACTEC 9000 MB system is a rapid, less labor-intensive detection system which allows for higher levels of recovery of AFB than solid media. There is no risk of cross contamination, which is known to be the case for the BACTEC 460 system, and data management is greatly facilitated. As a whole, however, the BACTEC 9000 MB system should only be used in conjunction with solid media.
authors.affiliation: Department of Medical Microbiology, Swiss National Center for Mycobacteria, University of Zurich.
authors.forename: G E
authors.initials: GE
authors.lastname: Pfyffer
authors.forename: C
authors.initials: C
authors.lastname: Cieslak
authors.forename: H M
authors.initials: HM
authors.lastname: Welscher
authors.forename: P
authors.initials: P
authors.lastname: Kissling
authors.forename: S
authors.initials: S
authors.lastname: Rüsch-Gerdes
mesh_terms: D001431:Bacteriological Techniques / Q000295:instrumentation*; D003470:Culture Media / Q000378:metabolism; D005189:False Positive Reactions; D005453:Fluorescence; D006801:Humans; D009161:Mycobacterium / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D009164:Mycobacterium Infections / Q000175:diagnosis*; D011851:Radioactivity; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013997:Time Factors
chemical_list: D003470:Culture Media