title: A high-fat diet influences insulin-stimulated posttransport muscle glucose metabolism in rats.
abstract: Because of a failure to detect significant quantities of intracellular glucose, it has been generally accepted that transport rather than phosphorylation is the rate-limiting process of muscle glucose metabolism under most (but not all) physiological conditions. Here, we have measured tissue free levels of the glucose analog 2-deoxy-D-glucose (2DG) in red quadriceps muscle of rats fed a high-fat diet (59% of energy from fat) for 3 weeks, to identify the barrier to insulin-stimulated glucose uptake previously seen in such animals. Measurements were performed on pentobarbital-anesthetized rats following exogenous infusion of radiolabeled 2DG. A glucose clamp was used to maintain plasma insulin at high physiological levels (approximately 120 mU/L). Three other treatment groups representing normal insulin action (chow-fed), extreme glucose uptake (maximal insulin stimulation + hyperglycemia), and insulin resistance with elevated free intracellular glucose (epinephrine infusion) were also studied for comparison. In chow-fed animals, no muscle free 2DG was detected, confirming transport as the rate-limiting process. In fat-fed animals, a significant elevation in muscle free 2DG was observed (P < .01 v chow-fed controls). The elevation was similar in magnitude to that in epinephrine-infused rats, and implied a limitation of insulin action at a posttransport step. This result was confirmed with a more complex modeling analysis. We conclude that posttransport steps influence insulin-stimulated in vivo muscle glucose metabolism in long-term high-fat-fed rats.
authors.affiliation: Garvan Institute of Medical Research, St Vincent's Hospital, Sydney, NSW, Australia.
authors.forename: S M
authors.initials: SM
authors.lastname: Furler
authors.forename: N D
authors.initials: ND
authors.lastname: Oakes
authors.forename: A L
authors.initials: AL
authors.lastname: Watkinson
authors.forename: E W
authors.initials: EW
authors.lastname: Kraegen
mesh_terms: D000818:Animals; D001692:Biological Transport; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004041:Dietary Fats / Q000008:administration & dosage* / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D015309:Glucose Clamp Technique; D006943:Hyperglycemia / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007333:Insulin Resistance; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D004041:Dietary Fats; D007328:Insulin; D003847:Deoxyglucose; D005947:Glucose; D004837:Epinephrine