Chemoattractant factors (IP-10, MIP-1alpha, IL-16) mRNA expression in mononuclear cells from different tissues during acute SIVmac251 infection of macaques.
We have used semiquantitative RT-PCR to monitor the expression of mRNA encoding chemoattractant factors IP-10, MIP-1alpha, and IL-16 in freshly isolated peripheral blood mononuclear cells (PBMCs), lymph node mononuclear cells (LNMCs), and mononuclear cells obtained after bronchoalveolar lavages (BALMCs) of two cynomolgus macaques inoculated intravenously with a pathogenic isolate of simian immunodeficiency virus, SIVmac251. Concomitant with the peak of systemic viral replication (two weeks after experimental inoculation) and proinflammatory cytokine IL-6 mRNA expression, high levels of MIP-1alpha and IP-10 mRNA were produced in LNMCs and BALMCs. In BALMCs, in which we have reported a marked progressive overexpression of IFN-gamma mRNA coinciding with an increase in the CD8+ lymphocyte percentages, we noticed a progressive overexpression of IL-16 mRNA. Our results suggest the role of chemokines IP-10, MIP-1alpha, and IL-16 in the development of inflammatory and immune responses during the early stages of lentiviral infection.
Chéret
A
A
Centre de Recherches du Service de Santé des Armées Emile Pardé, Commissariat à l'Energie Atomique, DSV/DRM, Institut Paris-Sud sur lesCytokines, Fontenay aux Roses, France.
Le Grand
R
R
Caufour
P
P
Neildez
O
O
Matheux
F
F
Théodoro
F
F
Boussin
F
F
Vaslin
B
B
Dormont
D
D
D000818:Animals; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054357:Chemokine CXCL10; D018925:Chemokines / Q000096:biosynthesis*; D019743:Chemokines, CXC; D007111:Immunity, Cellular; D007249:Inflammation / Q000276:immunology; D019410:Interleukin-16 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000821:virology; D008198:Lymph Nodes / Q000166:cytology; D008252:Macaca fascicularis; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054357:Chemokine CXCL10; D018925:Chemokines; D019743:Chemokines, CXC; D019410:Interleukin-16; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger