HPLC determination of flumethrin, deltamethrin, cypermethrin, and cyhalothrin residues in the milk and blood of lactating dairy cows.
A procedure to determine residue concentrations of synthetic pyrethroid insecticides (flumethrin, deltamethrin, cypermethrin and cyhalothrin) in the milk and blood of lactating dairy cows was developed. Extraction was performed with acetoritrile, n-hexane partitioning, and silica gel column cleanup with n-hexane and diethyl ether. Analysis was carried out by high-performance liquid chromatography and ultraviolet detection. Recovery of the four pyrethroids averaged 78 to 91% with a minimum detectable concentration of 0.001 mg/kg. The method was reproducible and sensitive.
Bissacot
D Z
DZ
Center for Toxicological Assistance (CEATOX), Institute of Biosciences, UNESP, Botucatu, São Paulo, Brazil.
Vassilieff
I
I
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D007306:Insecticides / Q000032:analysis* / Q000097:blood*; D007774:Lactation / Q000502:physiology*; D008892:Milk / Q000737:chemistry*; D009570:Nitriles; D010573:Pesticide Residues / Q000032:analysis* / Q000097:blood*; D011722:Pyrethrins / Q000032:analysis* / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D007306:Insecticides; D009570:Nitriles; D010573:Pesticide Residues; D011722:Pyrethrins; C017160:cypermethrin; C017180:decamethrin; C041392:flumethrin; C037304:cyhalothrin