Ribosomal RNA synthesis in Streptomyces lividans under heat shock conditions.
Clones containing rRNA genes were isolated from a gene library of Streptomyces lividans when RNA produced under heat shock conditions was used as a probe. Two of the clones carried entire rRNA operons rrnA and rrnF, respectively, the expression of both operons being under the control of four different promoters. At least two of the promoters were fully functional when the temperature increased from 30 to 45 degrees C, ensuring transcription of the rRNA genes under the heat shock. A third clone carried a partial rRNA operon in which expression was controlled by a main promoter that was functional at both 30 and 45 degrees C.
de León
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Centro Nacional de Biotecnología (CSIC), Campus de la Universidad Autónoma, Cantoblanco, Madrid, Spain.
Mellado
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D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D015723:Gene Library; D005798:Genes, Bacterial; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D012335:RNA, Ribosomal / Q000096:biosynthesis* / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics / Q000502:physiology*; D013696:Temperature; D014158:Transcription, Genetic; D012407:rRNA Operon
D012329:RNA, Bacterial; D012335:RNA, Ribosomal