Growth factors in the repair of partial thickness porcine skin wounds.
In 28 porcine partial thickness excisional wounds, the presence of several growth factors was first studied by enzyme-linked immunoadsorbent assay on wound fluid collected in sealed wound chambers. Basic fibroblast growth factor (bFGF) peaked on day 1 at 31.4 pg/ml; platelet derived growth factor (PDGF)-AB on day 3 reached 45.2 pg/ml, and transforming growth factor-beta (TGF-beta) on day 7 was 726.1 pg/ml. The same chamber system was used in 48 partial thickness excisional wounds for delivery of nanogram doses of bFGF, PDGF-AB, insulin-like growth factor (IGF)-1, epidermal growth factor (EGF), and cholera toxin. PDGF and EGF accelerated healing (1.1 days and 0.3 days, respectively), whereas bFGF and IGF-1 had no effect. Cholera toxin retarded healing by 1.9 days. Furthermore, in 100 excisional wounds EGF in the concentration range of 10 to 1,000 ng/ml had the same stimulating effect on healing. EGF at 10,000 ng/ml significantly delayed healing. The wound chamber model is useful for detecting of endogenous growth factors as well as for delivering exogenous factors.
Breuing
K
K
Brigham/Children's/Harvard Division of Plastic Surgery, Brigham and Women's Hospital, Boston, Mass, USA.
Andree
C
C
Helo
G
G
Slama
J
J
Liu
P Y
PY
Eriksson
E
E
D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology; D062109:Dermatologic Surgical Procedures; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005122:Exudates and Transudates / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000494:pharmacology; D006133:Growth Substances / Q000032:analysis / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D012038:Regeneration; D012867:Skin / Q000473:pathology; D012879:Skin Physiological Phenomena; D013552:Swine; D016212:Transforming Growth Factor beta / Q000032:analysis; D014945:Wound Healing
D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D002772:Cholera Toxin