The xylose-rich pectins from pea hulls.
The hot acid extract of pea hull, HSP, was rich in galacturonic acid, arabinose and xylose. It was fractionated by copper precipitation followed by ion-exchange chromatography. The copper-soluble fraction represented 26% of HSP and was mostly composed of an arabinan with a low degree of branching, some heteroxylans and a glucan, probably starch. The copper-precipitate (74% of HSP) contained pectins and some residual arabinan, xylan, glucan and mannan. One of the pectic fractions was rich in terminal xylose and fucose; it could be partially degraded by endo-polygalacturonase but not by endo-xylanase and seemed to contain xylogalacturonans.
Renard
C M
CM
Institut National de la Recherche Agronomique, Centre de Recherches Agro-alimentaires, Laboratoire de Biochimie et Technologie des Glucides, Nantes, France.
Weightman
R M
RM
Thibault
J F
JF
D002236:Carbohydrate Conformation; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008745:Methylation; D018532:Pisum sativum / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D043325:Xylan Endo-1,3-beta-Xylosidase; D014994:Xylose; D014995:Xylosidases / Q000378:metabolism
D010936:Plant Extracts; D003300:Copper; D010368:Pectins; D014994:Xylose; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase