title: Normal immunologic response to a neoantigen, bacteriophage phiX-174, in baboons with long-term lymphohematopoietic reconstitution from highly purified CD34+ Lin- allogeneic marrow cells.
abstract: The CD34 antigen is thought to be expressed by hematopoietic stem cells in adult humans and nonhuman primates. We present data that baboons transplanted with highly purified allogeneic CD34+ marrow cells devoid of detectable mature and immature T and B lymphocytes and myeloid cells, isolated from sex-mismatched mixed lymphocyte culture (MLC) nonreactive siblings, have maintained stable lymphohematopoietic engraftment with donor cells for greater than 4.9, greater than 6.0, and 5.0 years. Cytogenetic analysis of unfractionated marrow and peripheral blood cells at multiple time points after transplantation show virtually all donor cells in two animals and stable mixed chimerism in the third. We used polymerase chain reaction to show that colony-forming unit-granulocyte-macrophage, burst-forming unit-erythroid, and high proliferative potential colony-forming cells (HPP-CFC) were virtually all of donor origin in two animals and present at lower levels in the stable mixed chimera. CD20+ B-lymphoblastoid cell lines derived by Herpesvirus Papio transformation of peripheral blood cells were virtually all donor in two animals and 50% donor in the mixed chimera. CD4+ and CD8+ T cells and neutrophils purified from the peripheral blood of the two female animals also were all donor-derived. To assess immunologic function after transplantation, we immunized the three long-term chimeric animals and two normal control animals with bacteriophage phiX-174, a neoantigen that requires the interaction of antigen-presenting cells, T lymphocytes, and B lymphocytes to mount a normal antibody response. Experimental and control animals, when immunized with bacteriophage, had similar serum Ig levels. The experimental and control animals generated similar titers of antibacteriophage antibodies after primary and secondary immunizations with evidence of amplification and class switching. These findings further support the hypothesis that the CD34+ antigen is expressed on hematopoietic stem cells that can mediate stable long-term lymphohematopoiesis in vivo and, importantly, that normal immunologic function can be reconstituted in vivo after transplantation of the highly purified CD34+ Lin- cells alone.
authors.affiliation: Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Andrews
authors.forename: A
authors.initials: A
authors.lastname: Winkler
authors.forename: J
authors.initials: J
authors.lastname: Potter
authors.forename: E
authors.initials: E
authors.lastname: Bryant
authors.forename: G H
authors.initials: GH
authors.lastname: Knitter
authors.forename: I D
authors.initials: ID
authors.lastname: Bernstein
authors.forename: H D
authors.initials: HD
authors.lastname: Ochs
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D018952:Antigens, CD34 / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes; D010584:Bacteriophage phi X 174 / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005260:Female; D006410:Hematopoiesis; D008297:Male; D010215:Papio; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes
chemical_list: D000914:Antibodies, Viral; D018952:Antigens, CD34; D000956:Antigens, Viral