A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples.
A new method for the purification of protozoan cysts from feces was established, allowing to isolation of native cysts. The procedure consists of two sucrose-density gradients and enzymatic digestion of cellulose particles by cellulase and can be accomplished in a few hours. The cyst fractions were differentiated into Entamoeba histolytica and E. dispar using the DNA probes P145 and B133 and a dot-blot test.
Walderich
B
B
Institut für Tropenmedizin, Forschungslaboratorien, Tübingen, Germany. brigitte.walderich@uni-tuebingen.de
Müller
L
L
Bracha
R
R
Knobloch
J
J
Burchard
G D
GD
D000818:Animals; D002482:Cellulose / Q000032:analysis; D002499:Centrifugation, Density Gradient; D003560:Cysts; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis; D004747:Entamoeba / Q000145:classification / Q000302:isolation & purification*; D004748:Entamoeba histolytica / Q000145:classification / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000469:parasitology; D005243:Feces / Q000469:parasitology*; D006801:Humans; D008722:Methods
D015342:DNA Probes; D016054:DNA, Protozoan; D002482:Cellulose