[Inhibitory effects of malignant phenotype of human bladder cancer cell line by c-Ha-ras, c-myc antisense oligodeoxynucleotide].
The effects of two antisense oligodeoxynucleotide on expression of c-Ha-ras, c-myc proto-oncogene and the growth of human bladder cancer cell line were observed. Synthetic 15-mer directed at the region of the translational initiation site of c-Ha-ras, c-myc proto-oncogene (ASO-r, ASO-m) greatly inhibited the proliferation and DNA synthesis of T24 cell line, inhibited the expression of rasp21, mycp62 protein. The power of tumorigenesis of cell line treated with ASO was decreased. The results implicate the potential value in bladder cancer gene therapy.
Li
M
M
Third Military Hospital, People's Liberation Amry, Baoji.
Yu
M
M
Jin
F
F
D000818:Animals; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005796:Genes; D016259:Genes, myc; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D051575:Proto-Oncogene Proteins c-yes; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D014407:Tumor Cells, Cultured / Q000187:drug effects; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology; D019061:src-Family Kinases
D004273:DNA, Neoplasm; C000715470:MAS1 protein, human; D016376:Oligonucleotides, Antisense; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D051575:Proto-Oncogene Proteins c-yes; C496639:Yes1 protein, mouse; D019061:src-Family Kinases; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)