title: Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice.
abstract: We have recently shown that Flt3 ligand administration dramatically increases dendritic cell (DC) numbers in various mouse tissues. This has enabled the identification of distinct mature DC subpopulations. These have been designated: population C (CD11c(bright) CD11b(bright)), D (CD11c(bright) CD11b(dull)), and E (CD11c(bright) CD11b(negative)) This report demonstrates that the mature DC subsets (C, D, and E) from Flt3 ligand-treated mice differ with respect to phenotype, geographic localization, and function. The myeloid Ags CD11b, F4/80, and Ly-6C are predominantly expressed by population C, but not D or E. In addition, a subset of population C-type DC expresses 33D1 and CD4. In contrast, DC within population D and E selectively express the lymphoid-related DC markers CD8alpha, DEC 205, CD1d, as well as CD23, elevated levels of CD117 (c-kit), CD24 (HSA), CD13, and CD54. Immunohistology indicates that the different DC subsets reside in distinct microenvironments, with populations D and E residing in the T cell areas of the white pulp, while DC within population C localize in the marginal zones. These DC subpopulations showed different capacities to phagocytose FITC-zymosan and to secrete IL-12 upon stimulation with Staphylococcus aureus cowan I strain + IFN-gamma + granulocyte-macrophage-CSF. Population C-type DC were more phagocytic but secreted little inducible IL-12 while population D- and E-type DC showed poor phagocytic capacity and secreted considerably higher levels of IL-12. These results underscore the importance of viewing DC development in vivo, as an interplay between distinct lineages and a maturational dependence on specific microenvironmental signals.
authors.affiliation: Immunex Corporation, Seattle, WA 98101, USA. bpulendran@immunex.com
authors.forename: B
authors.initials: B
authors.lastname: Pulendran
authors.forename: J
authors.initials: J
authors.lastname: Lingappa
authors.forename: M K
authors.initials: MK
authors.lastname: Kennedy
authors.forename: J
authors.initials: J
authors.lastname: Smith
authors.forename: M
authors.initials: M
authors.lastname: Teepe
authors.forename: A
authors.initials: A
authors.lastname: Rudensky
authors.forename: C R
authors.initials: CR
authors.lastname: Maliszewski
authors.forename: E
authors.initials: E
authors.lastname: Maraskovsky
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology* / Q000276:immunology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D018664:Interleukin-12 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000494:pharmacology; D008565:Membrane Proteins / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018448:Models, Immunological; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology
chemical_list: D000943:Antigens, Differentiation; D008565:Membrane Proteins; D011994:Recombinant Proteins; C084881:flt3 ligand protein; D018664:Interleukin-12; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor