Identification of glucuronan lyase from a mutant strain of Rhizobium meliloti.
The Rhizobium meliloti M5N1CS (NCIMB 40472) mutant strain wich induces nodule formation on alfalfa roots, produces a (1 --> 4)-beta-D-glucuronan partially acetylated. During fermentation under specific conditions, the molecular weight of the polymer decrease, the presence of polysaccharide degrading enzyme was suspected. A glucuronan lyase was identified, this new bacterial lyase produces d.p. 4 oligoglucuronans, substituents (acetates) present on the substrate reduced the enzyme activity.
Michaud
P
P
LBM, IUT d'Amiens, France.
Pheulpin
P
P
Petit
E
E
Séguin
J P
JP
Barbotin
J N
JN
Heyraud
A
A
Courtois
B
B
Courtois
J
J
D000107:Acetylation; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000201:enzymology* / Q000235:genetics*
D002241:Carbohydrates; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C088556:mucoric acid; D011133:Polysaccharide-Lyases; C109209:glucuronan lyase