An overview on chemical modification of enzymes. The use of group-specific reagents.
Chemical modification is an important approach to the study of enzyme active sites. This article presents an overview of the methods used. The basic concepts, applications and limitations of chemical modification are outlined. The use of reagents specific for different amino acid side chains is also discussed.
Eyzaguirre
J
J
Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Santiago, Chile. jeyzag@genes.bio.puc.cl
D000495:Allosteric Site / Q000502:physiology; D000596:Amino Acids / Q000737:chemistry*; D004789:Enzyme Activation / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D017434:Protein Structure, Tertiary
D000596:Amino Acids