title: Study by ribosomal DNA ITS 2 sequencing and RAPD analysis on the systematics of four Metastrongylus species (Nematoda:Metastrongyloidea).
abstract: Four species of lungworms of the Metastrongylus genus are found sympatrically in the lungs of the wild boars (Sus scrofa L.) on the Chambord game reserve (France): Metastrongylus asymmetricus, M. confusus, M. pudendotectus, and M. salmi. These species are difficult to identify using morphological characters alone, and epidemiological evidence raises doubt about the existence of 4 distinct species. Two molecular approaches (sequencing of ribosomal DNA Internal Transcribed Spacer 2 [ITS 2] and Random Amplified Polymorphic DNA assay [RAPD]) were used to evaluate the genetic similarities between the 4 taxa. The ITS 2 sequences of M. salmi and M. confusus were identical, whereas the sequences of the other species were distinct. On the other hand, RAPD analysis indicated unambiguously that the 4 species are genetically different. These results reveal the limitation of using only 1 molecular approach for taxonomic studies and indicate the need to examine different regions (ribosomal, mitochondrial DNA) in the genome of a species in complement with morphological and epidemiological data.
authors.affiliation: INRA, Station de Pathologie Aviaire et de Parasitologie, Nouzilly, France.
authors.forename: V
authors.initials: V
authors.lastname: Leignel
authors.forename: J F
authors.initials: JF
authors.lastname: Humbert
authors.forename: L
authors.initials: L
authors.lastname: Elard
mesh_terms: D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D017931:DNA Primers / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry* / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry* / Q000235:genetics; D004276:DNA, Satellite / Q000737:chemistry / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D014644:Genetic Variation; D008168:Lung / Q000469:parasitology; D008174:Lung Diseases, Parasitic / Q000469:parasitology / Q000662:veterinary; D008297:Male; D008681:Metastrongyloidea / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012091:Repetitive Sequences, Nucleic Acid; D015203:Reproducibility of Results; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D017206:Strongylida Infections / Q000469:parasitology / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D004275:DNA, Ribosomal; D004276:DNA, Satellite