Assessment of the acrylic resin Technovit 7200 VLC for studying the gingival mucosa by light and electron microscopy.
Technovit 7200 VLC is an acrylic resin formulated for embedding undecalcified hard tissues which are prepared for light microscopy according to a cutting-grinding technique. To employ this resin for embedding and cutting soft tissues by ultramicrotomy, we carried out a qualitative study on biopsies of canine gingival mucosa using light and transmission electron microscopy. For a critical evaluation of this resin, some biopsies were embedded in Agar 100, an epoxy resin widely used in morphological studies. At the light microscopic level the samples embedded in Technovit 7200 VLC showed good morphology and excellent toluidine blue staining of different cell types and extra cellular matrix. At the ultrastructural level, nuclei, cytoplasmic organelles, collagen fibrils and ground substance appeared well preserved and showed high electron density. The acrylic resin was stable under the electron beam and its degree of shrinkage appeared to be very low. We conclude that Technovit 7200 VLC can be employed for ultramicrotomy for both light and electron microscopic investigation of soft tissues.
Botti
F
F
Department of Public Health & Cell Biology, School of Dentistry, University of Rome Tor Vergata, Italy.
Ragazzoni
E
E
Pilloni
A
A
Cocchia
D
D
D000180:Acrylic Resins; D000818:Animals; D004285:Dogs; D005881:Gingiva / Q000648:ultrastructure*; D008853:Microscopy; D008854:Microscopy, Electron; D009061:Mouth Mucosa / Q000648:ultrastructure*; D016613:Plastic Embedding / Q000379:methods*
D000180:Acrylic Resins