First isolation and characterization in humans of Entamoeba histolytica (laboratory-made) zymodeme XX.
Isoenzyme analysis by starch-gel electrophoresis has proved to be a useful method for the biochemical differentiation of pathogenic Entamoeba histolytica and non-pathogenic E. dispar isolates. Of the known 24 zymodemes, 3 are laboratory-made and have not previously been identified in humans. Parasitology screening was carried out in a psychiatric institution. Two amebic stocks were isolated and characterized that had never previously been found in humans and that have protein patterns identical to that of the laboratory-made zymodeme XX.
Gatti
S
S
Laboratory of Clinical Parasitology, University-IRCCS San Matteo, Pavia, Italy.
Cevini
C
C
Bruno
A
A
Ramsan
M
M
Marchi
L
L
Scaglia
M
M
D000328:Adult; D000818:Animals; D004748:Entamoeba histolytica / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000469:parasitology*; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D006593:Hexokinase / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D008297:Male; D010733:Phosphoglucomutase / Q000032:analysis
D007527:Isoenzymes; D008291:Malate Dehydrogenase; D006593:Hexokinase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase