Birch pollen profilin: structural organization and interaction with poly-(L-proline) peptides as revealed by NMR.
The secondary structure of birch pollen profilin, a potent human allergen, was elucidated by multidimensional nuclear magnetic resonance (NMR), as a prerequisite to study the interaction of this profilin with ligands for its poly-(L-proline) (PLP)-binding site. The chemical shifts of the 15N-labeled backbone amide groups were used to monitor complex formation with various PLP peptides. Titration with deca-L-proline (P10) yielded a KD of 0.2 mM. P8 was the shortest PLP to provoke a significant reaction. (GP5)3G bound significantly, confirming the interaction between profilins and the protein VASP containing this motif. Birch profilin interacted also with GP6GP5, found in the cyclase-associated protein (CAP), a suspected profilin ligand.
Domke
T
T
Molecular Structure Research, Gesellschaft für Biotechnologische Forschung, Braunschweig, Germany.
Federau
T
T
Schlüter
K
K
Giehl
K
K
Valenta
R
R
Schomburg
D
D
Jockusch
B M
BM
D000048:Acanthamoeba / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003001:Cloning, Molecular; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D004926:Escherichia coli; D009682:Magnetic Resonance Spectroscopy; D008840:Microfilament Proteins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D011058:Pollen / Q000737:chemistry*; D051304:Profilins; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014197:Trees; D000098662:Vasodilator-Stimulated Phosphoprotein
C081782:Cap1 protein, rat; D015815:Cell Adhesion Molecules; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D010455:Peptides; D010750:Phosphoproteins; D010940:Plant Proteins; D051304:Profilins; D011506:Proteins; D011994:Recombinant Proteins; D000098662:Vasodilator-Stimulated Phosphoprotein; C011083:polyproline