Activation of transcription factor NF-kappa B by phagocytic stimuli in human neutrophils.
Phagocytosis represents an important physiological trigger for the inducible expression of several genes in human neutrophils. Here, we report that a DNA-binding activity primarily consisting of the classical NF-kappa B heterodimer, p50/RelA, is induced in phagocytosing neutrophils. Under these conditions, NF-kappa B activation was found to be a rapid and transient response, reaching a maximum by 10-15 min, and returning to near-basal levels by 30 min. In neutrophils undergoing the phagocytosis of opsonized yeasts, the onset of NF-kappa B activation was paralleled by a decline in immunoreactive I kappa B-alpha protein levels, and the cellular I kappa B-alpha pool was replenished by 30 min, in agreement with our gel shift data. We conclude that NF-kappa B activation could constitute one of the mechanisms whereby the expression of kappa B-responsive genes is enhanced in phagocytosing neutrophils. To our knowledge, this represents the first demonstration that phagocytic stimuli can induce NF-kappa B activation in human neutrophils.
McDonald
P P
PP
Istituto di Patologia Generale, Università di Verona, Italy.
Cassatella
M A
MA
D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G; D016328:NF-kappa B / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D009504:Neutrophils / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D009895:Opsonin Proteins; D010587:Phagocytosis; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D051841:Transcription Factor RelB; D014157:Transcription Factors; D015533:Transcriptional Activation / Q000276:immunology
D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D016328:NF-kappa B; D009895:Opsonin Proteins; D011518:Proto-Oncogene Proteins; C497593:RELB protein, human; D014157:Transcription Factors; D051841:Transcription Factor RelB