title: Use of transabdominal ultrasound-guided amniocentesis for detection of equid herpesvirus 1-induced fetal infection in utero.
abstract: OBJECTIVE
  To evaluate transabdominal ultrasound-guided amniocentesis for detection of equid herpes-virus 1 (EHV-1)-induced fetal infection in utero.
  
  
  METHODS
  4 Welsh Mountain mares.
  
  
  METHODS
  Pregnant mares were inoculated intranasally with EHV-1 during the ninth month of gestation. Amniocentesis was initiated on postinoculation day (PID) 12, and was performed at 2- to 3-day intervals in standing mares under deep sedation. Amniotic fluid samples were tested by virus isolation (VI), polymerase chain reaction (PCR), and immunoperoxidase cytologic examination (IC) for detection of EHV-1.
  
  
  RESULTS
  Exposure to EHV-1 in the ninth month of gestation resulted in nasal shedding of infective virus, establishment of cell-associated viremia, and seroconversion. Equid herpesvirus 1 was detected by VI, PCR, and IC in amniotic fluid collected on PID 14 from 1 mare and on PID 16 and 17 from a second mare. Specimens of amniotic fluid from a third mare were VI negative until PID 18, when collections ceased, although this mare subsequently aborted an EHV-1-infected fetus on PID 28. The fourth mare aborted an EHV-1 infected fetus on PID 14. The 2 mares with VI-positive amniotic fluid were each carrying an EHV-1 infected fetus in utero, confirmed by examination of the uterus, placenta, and fetus, using specific immunohistochemistry and in situ hybridization. Endothelial cells in the endometrium and allantochorion were often virus-infected, with accompanying vascular lesions. The fetus had been infected via the chorionic vasculature in the first and fourth mares, and by inhalation of infected amniotic fluid in the second mare.
  
  
  CONCLUSIONS
  Amniocentesis permits specific detection of EHV-1-induced fetal infection in utero.
  
  
  CONCLUSIONS
  Amniocentesis may have a clinical role in the specific identification and isolation of mares carrying virus-infected fetuses during EHV-1-induced abortion epizootics.
authors.affiliation: Animal Health Trust Centre for Preventive Medicine, Suffollk, England.
authors.forename: K C
authors.initials: KC
authors.lastname: Smith
authors.forename: A J
authors.initials: AJ
authors.lastname: McGladdery
authors.forename: M M
authors.initials: MM
authors.lastname: Binns
authors.forename: J A
authors.initials: JA
authors.lastname: Mumford
mesh_terms: D000649:Amniocentesis / Q000379:methods / Q000662:veterinary*; D000653:Amniotic Fluid / Q000166:cytology; D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000196:embryology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000302:isolation & purification; D006734:Horse Diseases; D006736:Horses; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D016216:Ultrasonography, Prenatal / Q000379:methods / Q000662:veterinary*; D014766:Viremia / Q000175:diagnosis / Q000196:embryology / Q000662:veterinary
chemical_list: D004279:DNA, Viral