title: Value of the preoperative detection of prostate-specific-antigen-positive circulating cells by nested RT-PCR in patients submitted to radical prostatectomy.
abstract: OBJECTIVE
  To assess the value of the detection of circulating prostate cells [prostate-specific antigen (PSA) positive] by reverse-transcriptase nested polymerase chain reaction (nested RT-PCR) to improve the staging of clinically localized prostate cancer.
  
  
  METHODS
  Nested PCR was performed on blood samples of 29 patients submitted to radical prostatectomy for clinically localized (T1-T2) prostate cancer. Nine patients with various benign urologic diseases comprised the negative control group. Incubation was for 25 cycles for each PCR, using beta 2-microglobulin to test the integrity of RNA samples. Each sample was tested in quadruplicate and analyzed by agarose gel electrophoresis, blotted and hybridized with specific internal primers. Nested PCR results were compared with the pT stage of the prostate specimen, processed according to the Stanford method.
  
  
  RESULTS
  In 6 out of 29 patients (20.7%) with clinically localized prostate cancer, circulating prostate cells were detected by nested PCR. There was no relationship between pathologic stage and RT-PCR results. Eleven out of 14 pT2 patients (78.6%) were PCR negative and only 3 out of 15 pT3 patients (20%) were PCR positive. All control samples were PCR negative.
  
  
  CONCLUSIONS
  In selected patients with T1-T2 prostate cancer, there was no relationship between pathologic stage and the presence of circulating PSA-positive cells detected by nested PCR. However, in 20.7% of patients with clinically localized prostate cancer, circulating prostate cancer cells were detected. A further follow-up based on PSA is necessary to clarify the clinical relevance of this biologic anomaly.
authors.affiliation: Pharmacology Laboratory, Saint Louis Hospital, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: de Cremoux
authors.forename: V
authors.initials: V
authors.lastname: Ravery
authors.forename: M P
authors.initials: MP
authors.lastname: Podgorniak
authors.forename: S
authors.initials: S
authors.lastname: Chevillard
authors.forename: M
authors.initials: M
authors.lastname: Toublanc
authors.forename: N
authors.initials: N
authors.lastname: Thiounn
authors.forename: R
authors.initials: R
authors.lastname: Tatoud
authors.forename: V
authors.initials: V
authors.lastname: Delmas
authors.forename: T
authors.initials: T
authors.lastname: Calvo
authors.forename: L
authors.initials: L
authors.lastname: Boccon-Gibod
mesh_terms: D000368:Aged; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017430:Prostate-Specific Antigen / Q000097:blood* / Q000235:genetics; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000097:blood / Q000601:surgery*; D012313:RNA / Q000032:analysis; D014158:Transcription, Genetic / Q000235:genetics; D001613:beta 2-Microglobulin
chemical_list: D001613:beta 2-Microglobulin; D012313:RNA; D017430:Prostate-Specific Antigen