title: MR566A and MR566B, new melanin synthesis inhibitors produced by Trichoderma harzianum. I. Taxonomy, fermentation, isolation and biological activities.
abstract: New melanin synthesis inhibitors (MR566A and B) and six related known isocyanocyclopentenes were isolated from the fermentation broth of Trichoderma harzianum. The IC50 values of MR566A and B against mushroom tyrosinase were 1.72 and 47 microM, respectively. They inhibited melanin biosynthesis in B16 melanoma cells with MIC values of 0.1 and 2.2 microM, respectively. Also isolated from the same culture extract of T. harzianum was a new oxazole (MR93B), which showed no inhibitory activity against mushroom tyrosinase at a concentration of 1,000 microg/ml.
authors.affiliation: Korea Research Institute of Bioscience & Biotechnology, Taejon.
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.forename: M C
authors.initials: MC
authors.lastname: Chung
authors.forename: H J
authors.initials: HJ
authors.lastname: Lee
authors.forename: K S
authors.initials: KS
authors.lastname: Bae
authors.forename: Y H
authors.initials: YH
authors.lastname: Kho
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000201:enzymology; D003517:Cyclopentanes / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005285:Fermentation; D008543:Melanins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D009570:Nitriles / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism; D014242:Trichoderma / Q000737:chemistry / Q000145:classification* / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D003517:Cyclopentanes; D004791:Enzyme Inhibitors; C107805:MR 566A; C107806:MR 566B; D008543:Melanins; D009570:Nitriles; D014442:Monophenol Monooxygenase