Structural studies of CV-70 polysaccharide.
The goal of this paper is the characterization of the chemical structure of the water-soluble polysaccharide, CV-70, produced by bacteria Beijerinckia sp. Beijerinckia sp. is a genus of gram-negative, aerobic bacteria, usually found in sugar cane root. The CV-70 polysaccharide was produced in a fermentation medium containing 5% sucrose as the carbon source, tryptose and salts, at 25 degrees C [1]. The polysaccharide was hydrolyzed with 2 N trifluoroacetic acid at 100 degrees C for 16 h, purified, and analyzed by HPLC. Index of refraction was used for the detection of sugars. For GC-MS analysis, the CV-70 polysaccharide was derivatized through methylation and acetylation. Together with the GC-MS data, periodate oxidation studies were used to determine the possible glucosidic linkages. Carbon-13 NMR studies were carried out with hydrolyzed and silylated samples. Glucose, galactose and fucose were identified as the components in the CV-70 polysaccharide, in a 3:1:3 ratio.
Scamparini
A
A
State University of Campinas, Food Engineering Faculty, Food Science Department, S.P. Brazil.
Mariuzzo
D
D
Fujihara
H
H
Jacobusi
R
R
Vendruscolo
C
C
D001419:Bacteria / Q000737:chemistry; D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism
D009005:Monosaccharides; D011135:Polysaccharides, Bacterial