Analysis of inositol phosphates in heart tissue using anion-exchange high-performance liquid chromatography.
The pathways of release and metabolism of inositol phosphates in intact heart tissue are different from those observed in isolated cardiomyocytes in culture. Thus, it is essential that methods are available for the quantitation of inositol phosphates in intact tissue preparations. This manuscript describes methods which allow the quantitation of inositol phosphates in different heart preparations including isolated atria and intact perfused heart. The availability of such methods should facilitate study of the role of inositol phosphates in cardiac control mechanisms under physiological and pathological conditions.
Woodcock
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EA
Cellular Biochemistry Laboratory, Baker Medical Research Institute, Prahran, Victoria, Australia.
D000818:Animals; D000837:Anion Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D005612:Freeze Drying; D006352:Heart Ventricles / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000737:chemistry* / Q000302:isolation & purification; D051379:Mice; D009206:Myocardium / Q000737:chemistry*; D010477:Perfusion; D051381:Rats; D014238:Trichloroacetic Acid
D000837:Anion Exchange Resins; D007295:Inositol Phosphates; D014238:Trichloroacetic Acid