title: Proliferative responses to PHA, anti-CD3 and antigens in patients with lymphoproliferative disease of granular lymphocytes. Mannoproteins of Candida albicans induce proliferation and cytotoxicity.
abstract: In this paper we investigated the lymphoproliferative and cytotoxic responses of peripheral blood mononuclear cells (PBMC) of patients affected with lymphoproliferative disease of granular lymphocytes of T cell origin to mitogens and antigens. Most patients with lymphoproliferative disease of granular lymphocytes (LDGL) showed a severely impaired PBMC proliferative capacity in response to phytohemagglutinin, anti-CD3 and tetanus toxoid, an impairment that, in the case of anti-CD3 response, appears to be related to a defect of phosphorylation in response to triggering. In contrast, at least 50% of the patients had normal proliferation in response to a mannoprotein fraction purified from Candida albicans. These data suggest that some CD3+ GL from LDGL patients, that usually respond poorly to proliferative stimuli in vitro, can be triggered to perform these functions by candidal antigens.
authors.affiliation: Department of Clinical Medicine, La Sapienza University of Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Giovannetti
authors.forename: I
authors.initials: I
authors.lastname: Quinti
authors.forename: L
authors.initials: L
authors.lastname: Rainaldi
authors.forename: F R
authors.initials: FR
authors.lastname: Mauro
authors.forename: A
authors.initials: A
authors.lastname: Oliva
authors.forename: M
authors.initials: M
authors.lastname: Pierdominici
authors.forename: G
authors.initials: G
authors.lastname: Ricci
authors.forename: G
authors.initials: G
authors.lastname: De Rossi
authors.forename: A
authors.initials: A
authors.lastname: Cassone
authors.forename: F
authors.initials: F
authors.lastname: Aiuti
authors.forename: F
authors.initials: F
authors.lastname: Pandolfi
mesh_terms: D000328:Adult; D000368:Aged; D000906:Antibodies / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008230:Lymphomatoid Granulomatosis / Q000276:immunology*; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D008875:Middle Aged; D010641:Phenotype; D010766:Phosphorylation; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology
chemical_list: D000906:Antibodies; D017252:CD3 Complex; D008562:Membrane Glycoproteins; D010835:Phytohemagglutinins; D013745:Tetanus Toxoid; C025363:mannoproteins