title: Late memory-related genes in the hippocampus revealed by RNA fingerprinting.
abstract: Although long-term memory is thought to require a cellular program of gene expression and increased protein synthesis, the identity of proteins critical for associative memory is largely unknown. We used RNA fingerprinting to identify candidate memory-related genes (MRGs), which were up-regulated in the hippocampus of water maze-trained rats, a brain area that is critically involved in spatial learning. Two of the original 10 candidate genes implicated by RNA fingerprinting, the rat homolog of the ryanodine receptor type-2 and glutamate dehydrogenase (EC 1.4.1.3), were further investigated by Northern blot analysis, reverse transcription-PCR, and in situ hybridization and confirmed as MRGs with distinct temporal and regional expression. Successive RNA screening as illustrated here may help to reveal a spectrum of MRGs as they appear in distinct domains of memory storage.
authors.affiliation: Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: S
authors.initials: S
authors.lastname: Cavallaro
authors.forename: N
authors.initials: N
authors.lastname: Meiri
authors.forename: C L
authors.initials: CL
authors.lastname: Yi
authors.forename: S
authors.initials: S
authors.lastname: Musco
authors.forename: W
authors.initials: W
authors.lastname: Ma
authors.forename: J
authors.initials: J
authors.lastname: Goldberg
authors.forename: D L
authors.initials: DL
authors.lastname: Alkon
mesh_terms: D000818:Animals; D005796:Genes; D005821:Genetic Techniques; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D008568:Memory / Q000502:physiology*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012313:RNA / Q000032:analysis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013550:Swimming / Q000502:physiology
chemical_list: D009419:Nerve Tissue Proteins; D012313:RNA