title: Changes of telomere length cause reciprocal changes in the lifespan of mother cells in Saccharomyces cerevisiae.
abstract: Budding yeast cells divide asymmetrically, giving rise to a mother and its daughter. Mother cells have a limited division potential, called their lifespan, which ends in proliferation-arrest and lysis. In this report we mutate telomerase in Saccharomyces cerevisiae to shorten telomeres and show that, rather than shortening lifespan, this leads to a significant extension in lifespan. This extension requires the product of the SIR3 gene, an essential component of the silencing machinery which binds to telomeres. In contrast, longer telomeres in a genotypically wild-type strain lead to a decrease in lifespan. These findings suggest that the length of telomeres dictates the lifespan by regulating the amount of the silencing machinery available to nontelomeric locations in the yeast genome.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, 68-280, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
authors.forename: N R
authors.initials: NR
authors.lastname: Austriaco
authors.forename: L P
authors.initials: LP
authors.lastname: Guarente
mesh_terms: D002470:Cell Survival / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D016681:Genome, Fungal; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D016615:Telomere / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; C084122:SIR3 protein, S cerevisiae; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D015534:Trans-Activators