title: Inactivation of hepatitis B virus: evaluation of the efficacy of the disinfectant 'Solprogel' using a DNA-polymerase activity assay.
abstract: The effects of sodium dichloroisocyanurate (NaDCC) and Solprogel (Laboratorios Inibsa, S.A., Barcelona, Spain), a compound that contains NaDCC plus a biodegradable polymer of acrylic acid, on the activity of DNA polymerase (DNA-P) associated with hepatitis B virus in serum were evaluated. DNA-P positive and negative pools of human serum samples were used as positive and negative stock virus. Inhibition of DNA-P activity by NaDCC and the commercial product was found to be concentration-dependent. Two minutes exposure to the minimum effective concentration of NaDCC (1000 ppm available chlorine) or Solprogel 16% (960 ppm available chlorine) totally inhibited DNA-P activity.
authors.affiliation: Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Hernández
authors.forename: F J
authors.initials: FJ
authors.lastname: Belda
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
authors.forename: L
authors.initials: L
authors.lastname: Matas
authors.forename: M
authors.initials: M
authors.lastname: Giménez
authors.forename: M
authors.initials: M
authors.lastname: Caraballo
authors.forename: C
authors.initials: C
authors.lastname: Ramil
authors.forename: V
authors.initials: V
authors.lastname: Ausina
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects; D004202:Disinfectants / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D005782:Gels; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D012996:Solutions; D014227:Triazines / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D004202:Disinfectants; D005782:Gels; D012996:Solutions; D014227:Triazines; D004259:DNA-Directed DNA Polymerase; C011765:troclosene