Lysosomal enzyme activities in cultured retinal pigment epithelial and glial cells of RCS rat.
OBJECTIVE
To compare the activities of acid phosphatase, N-acetyl-beta-glucosaminidase and alpha-mannosidase in cultured retinal pigment epithelium (RPE) and glial cells of Royal College of Surgeons (RCS) rat with those in Long Evans (LE).


METHODS
The cultured RPE and glial cells of RCS and LE rat were plated into the same 96 well microtitre, and the biochemical method in microsystem were used for enzyme assays.


RESULTS
The activities of acid phosphatase and N-acetyl-beta-glucosaminidase are higher by, respectively, 30% and 46% in cultured RPE of RCS rat than LE rat. The activity of alpha-mannosidase has no significant difference. The activities of 3 enzymes in the retinal glial cells derived from RCS rats are higher than LE rat by 13% to 77%.


CONCLUSIONS
These results suggest that the high activities of lysosomal enzymes in RCS RPE and glial cells may play an important role in the pathogenesis of retinal dystrophy.
Hu
J
J
Shen Zhen Eye Hospital, China.
Naka
H
H
Kuriyama
H
H
Kojima
S
S
Tsuboi
S
S
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D008361:Mannosidases / Q000378:metabolism; D009457:Neuroglia / Q000201:enzymology* / Q000473:pathology; D010857:Pigment Epithelium of Eye / Q000201:enzymology* / Q000473:pathology; D051381:Rats; D012174:Retinitis Pigmentosa / Q000201:enzymology*; D043323:alpha-Mannosidase
D000135:Acid Phosphatase; D008361:Mannosidases; D043323:alpha-Mannosidase; D000118:Acetylglucosaminidase