title: Propagation of human astrovirus in the PLC/PRF/5 hepatoma cell line.
abstract: Astroviruses are associated with gastroenteritis in humans and many diseases in animals. Human astroviruses (HAstVs) cannot be propagated readily or isolated in conventional cell cultures. The presence of trypsin supports HAstV growth in selected cell cultures such as the continuous colonic carcinoma cell line (CaCo-2). This study reports on the propagation of cell culture adapted reference strains of HAstV, and the direct isolation of HAstV from stool specimens in the human hepatoma cell line PLC/PRF/5.
authors.affiliation: Department of Medical Virology, University of Pretoria, South Africa. mtaylor@medic.up.ac.za
authors.forename: M B
authors.initials: MB
authors.lastname: Taylor
authors.forename: W O
authors.initials: WO
authors.lastname: Grabow
authors.forename: W D
authors.initials: WD
authors.lastname: Cubitt
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000821:virology*; D005243:Feces / Q000821:virology; D006801:Humans; D008113:Liver Neoplasms / Q000821:virology*; D001257:Mamastrovirus / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D014407:Tumor Cells, Cultured; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D000956:Antigens, Viral