Further studies on the recoupling effect of 6-ketocholestanol upon oxidative phosphorylation in uncoupled liver mitochondria.
The effect of 6-ketocholestanol was studied on CCCP-induced uncoupling in liver mitochondria, submitochondrial particles and cytochrome oxidase proteoliposomes. It was found that 6-ketocholestanol prevents and reverses uncoupling induced by nM concentrations of CCCP on the three systems assayed. As it was reported on kidney mitochondrial membranes [Chavez et al. (1996) FEBS Lett. 379, 305-308], the recoupling effect caused by 6-ketocholestanol on submitochondrial particles and proteoliposomes could be due to a diminution of membrane fluidity.
Cuéllar
A
A
Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, México D.F., Mexico.
Ramirez
J
J
Infante
V M
VM
Chavez
E
E
D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004161:Diphenylhexatriene / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000378:metabolism; D007653:Ketocholesterols / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D011510:Proteolipids / Q000378:metabolism; D051381:Rats; D013367:Submitochondrial Particles / Q000378:metabolism; D013696:Temperature; D014475:Uncoupling Agents / Q000494:pharmacology
D005456:Fluorescent Dyes; D007653:Ketocholesterols; D011510:Proteolipids; D014475:Uncoupling Agents; C020485:proteoliposomes; D004161:Diphenylhexatriene; C063833:6-ketocholestanol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003576:Electron Transport Complex IV