Delayed neuronal death prevented by inhibition of increased hydroxyl radical formation in a transient cerebral ischemia.
The salicylate-trapping method was used to detect hydroxyl radicals by measurement of stable adduct dihydroxybenzoic acid (DHBA). Ten minutes of forebrain ischemia followed by reperfusion induced the increase in DHBA in rat hippocampal perfusates. Postischemic treatment with a free radical scavenger, 3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186), significantly reduced the increase in DHBA and suppressed delayed neuronal death in the hippocampal CA1 region.
Yamamoto
T
T
Pharmaceuticals Laboratory I, Yokohama Research Center, Mitsubishi Chemical Corp., Aoba-ku, Yokohama, Japan. ytosi@rc.m-kagaku.co.jp
Yuki
S
S
Watanabe
T
T
Mitsuka
M
M
Saito
K I
KI
Kogure
K
K
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives / Q000494:pharmacology; D002545:Brain Ischemia / Q000378:metabolism*; D016923:Cell Death / Q000502:physiology; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000494:pharmacology; D006624:Hippocampus / Q000098:blood supply* / Q000166:cytology / Q000378:metabolism; D062385:Hydroxybenzoates / Q000032:analysis / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000032:analysis / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D009474:Neurons / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
D016166:Free Radical Scavengers; D062385:Hydroxybenzoates; D007502:Iron Chelating Agents; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid; D000077553:Edaravone; D000983:Antipyrine