Sequence of two gonadotropin releasing hormones from tunicate suggest an important role of conformation in receptor activation.
The primary structure of two forms of gonadotropin releasing hormone (GnRH) from tunicate (Chelyosoma productum) have been determined based on mass spectrometric and chemical sequence analyses. The peptides, tunicate GnRH-I and -II, contain features unprecedented in vertebrate GnRH. Tunicate GnRH-I contains a putative salt bridge between Asp5 and Lys8. A GnRH analog containing a lactam bridge between Asp5 and Lys8 was found to increase release of estradiol compared with that of the native tunicate GnRH-I and -II. Tunicate GnRH-II contains a cysteine residue and was isolated as a dimeric peptide. These motifs suggest that the conformation plays an important role in receptor activation.
Craig
A G
AG
The Clayton Foundation, Laboratories for Peptide Biology, The Salk Institute, La Jolla, CA 92037, USA. craig@salk.edu
Fischer
W H
WH
Park
M
M
Rivier
J E
JE
Musselman
B D
BD
Powell
J F
JF
Reska-Skinner
S M
SM
Prakash
M O
MO
Mackie
G O
GO
Sherwood
N M
NM
D000595:Amino Acid Sequence; D000818:Animals; D019281:Dimerization; D004958:Estradiol / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D011966:Receptors, LHRH / Q000378:metabolism*; D017421:Sequence Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014561:Urochordata / Q000737:chemistry* / Q000378:metabolism
D010446:Peptide Fragments; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol