Collagen VII expression in human chronic wounds and scars.
Collagen VII is the major structural component of the anchoring fibrils that stabilize the cutaneous basement membrane on the dermis. Disruption and, more usually, destruction of the basement membrane are characteristic of wounds that are slow, or fail, to heal, such as chronic lower-limb or pressure ulcers. In this study, the expression of collagen VII was analysed in 28 human chronic cutaneous wounds or scars using a reverse transcription-polymerase chain reaction (RT-PCR) technique. Collagen VII expression was detected in 26 of these 28 cutaneous wounds, but not in two wounds, neither of which showed any clinical evidence of healing.
Hopkinson
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I
Wound Healing Research Unit, University of Wales College of Medicine, Cardiff, U.K. Ian_Hopkinson@MSN.co
Anglin
I E
IE
Evans
D L
DL
Harding
K G
KG
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D002908:Chronic Disease; D002921:Cicatrix / Q000378:metabolism*; D003094:Collagen / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007871:Leg Ulcer / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism*
D003094:Collagen