title: Chromatin condensation in erythropoiesis resolved by multipixel spectral imaging: differentiation versus apoptosis.
abstract: Chromatin condensation and nuclear organization of May-Grunwald-Giemsa (MGG)-stained normal erythropoietic bone marrow cells and apoptotic red cell precursors were resolved by spectral bio-imaging. Multipixel spectra were obtained from single cells displaying a range of wavelengths of both transmitted and absorbed light. Two groups of spectra, of low- and high-intensity transmitted light, were revealed in the nuclei of each cell. The absorbance spectra served for the reconstruction of "absorbance images" depicting the affinity of MGG stain for the chromatin of proerythroblasts and of basophilic, polychromatic, and orthochromatic normoblasts. The localization of different spectral components in the nuclei was resolved employing two mathematical methods, spectral similarity mapping and principal component analysis. Novel structures of high symmetry revealing windmill-like organization were detected in basophilic, polychromatic, and orthochromatic normoblast cells. Matching structures were detected in apoptotic normoblasts obtained from an agnogenic myeloid metaplasia patient. Apoptosis was associated with a gradual breakdown of the ordered arrays in the nucleus. We propose that DNA cleavage may lead to fragmentation of the symmetrical windmill-like superstructure of the basic nuclear domains.
authors.affiliation: Life Sciences Department, Bar Ilan University, Ramat-Gan, Israel.
authors.forename: C
authors.initials: C
authors.lastname: Rothmann
authors.forename: A M
authors.initials: AM
authors.lastname: Cohen
authors.forename: Z
authors.initials: Z
authors.lastname: Malik
mesh_terms: D017209:Apoptosis; D001399:Azure Stains; D002454:Cell Differentiation; D002843:Chromatin / Q000502:physiology*; D004900:Erythroblasts / Q000502:physiology* / Q000648:ultrastructure; D004920:Erythropoiesis; D006801:Humans; D008433:Mathematics; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D001399:Azure Stains; D002843:Chromatin