Pervanadate elicits proliferation and mediates activation of mitogen-activated protein (MAP) kinase in the nucleus.
There is growing evidence for the role of protein tyrosine phosphatases in controlling such fundamental cellular processes as growth and differentiation. Pervanadate is a potent inhibitor of protein tyrosine phosphatase which has been observed here to induce proliferation in C3H10T1/2 mouse fibroblasts. Pervanadate also translocated/activated p42/44 mitogen-activated protein (MAP) kinase to the cell nucleus. An almost similar pattern of nuclear p42/44 MAP kinase stimulation is seen with TPA. On the other hand, TPA treatment results in a rapid activation of cytosolic MAP kinase which declines with time. Thus pervanadate appears as a very useful tool for studying tyrosine phosphorylation.
Krady
M M
MM
Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, UPR 416 du CNRS, Strasbourg, France.
Freyermuth
S
S
Rogue
P
P
Malviya
A N
AN
D000818:Animals; D001692:Biological Transport; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology*; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008808:Mice, Inbred CBA; D014638:Vanadates / Q000494:pharmacology*
C076715:pervanadate; D014638:Vanadates; D017871:Calcium-Calmodulin-Dependent Protein Kinases