The incorporation, modification and turnover of fatty acids in adult Schistosoma mansoni.
Schistosoma mansoni is known to be unable to synthesize fatty acids and sterols de novo, but the parasite is capable of synthesizing phospholipids and triacylglycerols from precursors obtained from the host. The present study focuses on the dynamics of the incorporation of fatty acids in adult parasites. This study showed that fatty acids were rapidly metabolized into complex lipids and that oleate (18:1) was efficiently converted to eicosenoate (20:1) by chain elongation, whereas palmitate was not elongated at an appreciable rate. This chain elongation mainly involved fatty acids that were previously esterified to complex lipids. Furthermore it was shown that in adult parasites triacylglycerols do not serve as fatty-acyl donors in phospholipid synthesis as had been suggested to be the case in schistosomula, because: (1) Immediately after pulse-labelling the specific activity of fatty acids in phospholipids was higher than in triacylglycerols; and (2) the specific activity of eicosenoate, which had been formed by chain elongation of incorporated oleate. was higher in phospholipids than in triacylglycerols. Fatty acids that were esterified to phospholipids had a high turnover, in contrast to fatty acids esterified to triacylglycerols, which persisted for extended periods of time in this lipid class (days rather than hours).
Brouwers
J F
JF
Laboratory of Veterinary Biochemistry and Institute of Biomembranes, Utrecht University, The Netherlands.
Smeenk
I M
IM
van Golde
L M
LM
Tielens
A G
AG
D000215:Acylation; D000818:Animals; D004537:Eicosanoic Acids / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis / Q000737:chemistry; D019301:Oleic Acid / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis / Q000378:metabolism; D012550:Schistosoma mansoni / Q000254:growth & development / Q000378:metabolism*; D013261:Sterols / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis / Q000378:metabolism
D004537:Eicosanoic Acids; D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D013261:Sterols; D014280:Triglycerides; D019301:Oleic Acid; D019308:Palmitic Acid