Antibody cross-linking of the thymocyte-specific cell surface molecule CTX causes abnormal mitosis and multinucleation of tumor cells.
The thymocyte-specific cell surface molecule CTX is a developmentally regulated type I transmembrane protein of the immunoglobulin superfamily which, in the amphibian Xenopus, is exclusively expressed by a large fraction of cortical thymocytes and by different cell lines derived from independent spontaneous thymic tumors. Antibody cross-linking of CTX in vitro inhibits the growth of tumor cells and causes morphological alterations. Cells divide abnormally, accumulate in the G2/M phase of the cell cycle, and become multinucleated. This demonstrates, for the first time, that multinucleation can be induced by specifically cross-linking a cell surface molecule.
Robert
J
J
University of Rochester Medical Center, Rochester, New York 14642, USA. robert@uhura.cc.rochester.edu
Brown
D M
DM
Pasquier
L D
LD
Cohen
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology / Q000502:physiology*; D002453:Cell Cycle; D002467:Cell Nucleus / Q000473:pathology; D003432:Cross-Linking Reagents; D016195:G2 Phase; D008565:Membrane Proteins / Q000276:immunology / Q000502:physiology*; D008870:Microtubules / Q000473:pathology; D008938:Mitosis; D013601:T-Lymphocytes / Q000166:cytology*; D013953:Thymus Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured; D014981:Xenopus; D029867:Xenopus Proteins
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D003432:Cross-Linking Reagents; D008565:Membrane Proteins; D029867:Xenopus Proteins; C099384:ctx-A protein, Xenopus