title: Upregulation of phagocytosis and candidicidal activity of macrophages exposed to the immunostimulant acemannan.
abstract: Previous studies by these investigators have shown that mannosylated bovine serum albumin (m-BSA) enhances the respiratory burst (RB), phagocytosis, and killing of Candida albicans by resident murine peritoneal macrophages (MO). Upregulation of the above MO functions was associated with binding of m-BSA to the MO-mannose receptor. The present study was done to determine if the immunostimulant, acemannan prepared from aloe vera, could stimulate MO in a similar manner. Resident peritoneal MO collected from C57BL/6 mice were exposed to acemannan for 10 min. The RB was measured using chemiluminescence and demonstrated approximately a two-fold increase above the media controls. In studies involving phagocytosis, MO were exposed to acemannan, washed and exposed to Candida at a ratio of 1:5. The percent phagocytosis and Candida killing were determined using fluorescence microscopy. There was a marked increase in phagocytosis in the treated cultures (45%) compared to controls (25%). Macrophages exposed to acemannan for 10 min resulted in ca 38% killing of Candida albicans compared with 0-5% killing in controls. If MO were incubated with acemannan for 60 min, 98% of the yeast were killed compared to 0-5% in the controls. The results of the present study indicate that short term exposure of MO to acemannan upregulates the RB, phagocytosis and candidicidal activity. Further studies are needed to clarify the potential use of this immunostimulant as an anti-fungal agent.
authors.affiliation: Department of Biological Sciences, Texas Tech University, Lubbock 79409, U.S.A.
authors.forename: R W
authors.initials: RW
authors.lastname: Stuart
authors.forename: D L
authors.initials: DL
authors.lastname: Lefkowitz
authors.forename: J A
authors.initials: JA
authors.lastname: Lincoln
authors.forename: K
authors.initials: K
authors.lastname: Howard
authors.forename: M P
authors.initials: MP
authors.lastname: Gelderman
authors.forename: S S
authors.initials: SS
authors.lastname: Lefkowitz
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002176:Candida albicans / Q000276:immunology; D002417:Cattle; D005260:Female; D066298:In Vitro Techniques; D008262:Macrophage Activation / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology* / Q000502:physiology; D008297:Male; D008351:Mannans / Q000494:pharmacology*; D008358:Mannose; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000187:drug effects; D016897:Respiratory Burst / Q000187:drug effects; D012709:Serum Albumin; D015854:Up-Regulation
chemical_list: D000276:Adjuvants, Immunologic; D008351:Mannans; D012709:Serum Albumin; C023840:mannose-bovine serum albumin conjugate; D008358:Mannose; C058414:acemannan