Reusable, real-time, immuno-optical protein C biosensor.
A Protein C (PC) biosensor can be used to diagnose PC deficiency, to monitor the PC level in the blood of PC deficient patients, and to measure the PC concentration in other PC-containing samples, such as PC producing animal cell culture broth or transgenic animal milk. A fully functional biosensor requires extremely high sensitivity and specificity, and real-time measurement. To satisfy these requirements, it is proposed to develop an immuno-optical fiber biosensor that utilizes PC-specific biomolecules (PC probes) tagged with fluorophores. The method involves immobilizing monoclonal antibody against PC (anti-PC) on the surface of an optical fiber. When PC in a sample is adsorbed to the anti-PC on the fiber, it can be reached with the fluorophore tagged PC-probe. The intensity of light transported through the optical fiber, therefore, can be correlated with the concentration of PC in the sample. The sensor will be designed so it can be reused, following a simple elution step, thus reducing diagnostic expense. The preliminary study shows encouraging future for the real-time optical PC biosensor.
Kang
K A
KA
Department of Chemical and Biochemical Engr., University of Maryland Baltimore County (UMBC) 21228, USA.
Anis
N A
NA
Eldefrawi
M E
ME
Drohan
W
W
Bruley
D F
DF
D000818:Animals; D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques; D005069:Evaluation Studies as Topic; D005336:Fiber Optic Technology; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data; D055100:Optical Fibers; D011486:Protein C / Q000032:analysis*; D020151:Protein C Deficiency; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D011486:Protein C; D016650:Fluorescein-5-isothiocyanate