title: Complete RNA1 sequences of two UK isolates of barley mild mosaic virus: a wild-type fungus-transmissible isolate and a non-fungus-transmissible derivative.
abstract: The complete RNA1 sequences of two isolates (fungus transmissible and non-fungus transmissible) of barley mild mosaic virus (BaMMV) were obtained. The two isolates' RNA1 sequences had very high sequence identity (99.3%), and of the 15 amino acid differences (out of 2258) between the putative polyproteins, 11 were conservative and unlikely to affect the structure or function of the protein. The remaining amino acid differences were thought unlikely to affect fungus transmission because they occur in the CI- and NIb-coding regions. This strongly suggests that the P73 protein of RNA2 (which has a 364-aa deletion in the non-fungus-transmissible isolate) is involved in fungus transmission of BaMMV.
authors.affiliation: Max-Planck-Institut für Zuchtungsforschung, Abt. Genetische Grundlagender Pflanzenzuchtung, Köln, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Peerenboom
authors.forename: E J
authors.initials: EJ
authors.lastname: Cartwright
authors.forename: I
authors.initials: I
authors.lastname: Foulds
authors.forename: M J
authors.initials: MJ
authors.lastname: Adams
authors.forename: R
authors.initials: R
authors.lastname: Stratford
authors.forename: A
authors.initials: A
authors.lastname: Rosner
authors.forename: H H
authors.initials: HH
authors.lastname: Steinbiss
authors.forename: J F
authors.initials: JF
authors.lastname: Antoniw
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D005658:Fungi / Q000821:virology*; D001467:Hordeum / Q000382:microbiology / Q000821:virology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology / Q000821:virology*; D016133:Polymerase Chain Reaction; D017800:Potyvirus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D006113:United Kingdom
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral