Allospecific cytotoxic T cells generated from beta 2m-/- mice in primary MLC: analysis of activation requirements, specificity, and phenotype.
It has been demonstrated by several investigators that beta 2m-/- knockout mice are deficient in the expression of MHC Class I molecules but can nevertheless generate CD8(+) allospecific cytotoxic T cells following vigorous in vivo priming. We demonstrate here that in vivo priming is not necessary to generate MHC Class I allospecific CTL from beta 2m-/- mice. When splenocytes from naive unprimed beta 2m-/- mice were provided exogenous cytokines in MHC Class I disparate primary MLC, allospecific cytolytic effectors were generated. beta 2m-/- MHC Class I allospecific CTL that were CD3+ and Thy1.2+ were otherwise heterogeneous in phenotype, including CD8+, CD4+, CD8-CD4-, TCR alpha beta+, and TCR gamma delta+ T cells. This phenotypic variability of beta 2m-/- CTL generated in primary MLC reveals the diversity of CTL precursors that develop in vivo in the absence of MHC Class I.
Lamousé-Smith
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Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA.
McCarthy
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SA
D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002474:Cell-Free System / Q000276:immunology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D000939:Epitopes / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D016130:Immunophenotyping; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D017710:Lymphocyte Transfusion; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013154:Spleen / Q000276:immunology / Q000637:transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification* / Q000187:drug effects / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000276:immunology*
D019138:Antibodies, Blocking; D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D007519:Isoantigens; D001613:beta 2-Microglobulin; D003208:Concanavalin A