title: Percutaneous implantation of non-small-cell lung carcinoma: technique and observations.
abstract: OBJECTIVE
  The authors performed this study to determine whether intrathoracic inoculation of non-small-cell lung carcinoma with fluoroscopic guidance would provide for more accurate implantation.
  
  
  METHODS
  A tumor cell inoculum (2 x 10(6) cells per 0.15 mL) was injected percutaneously under fluoroscopic guidance at the posterior midaxillary line in 22 athymic nude mice. The mice underwent imaging with a mammographic unit at 3, 5, and 8 weeks after implantation. The mice were sacrificed at 8 weeks, and autopsy was performed to determine tumor yield.
  
  
  RESULTS
  The use of a percutaneous technique under fluoroscopic guidance greatly facilitated the accurate implantation of xenografts. Tumor growth was seen at radiography in 18 of the 22 (82%) mice at 8 weeks. Necropsy revealed a 100% tumor yield. Histologic examination confirmed adenocarcinoma of the lung. The average number of tumors found in the lung parenchyma was 1.05 +/- 0.35; the average number of tumors found in the mediastinum was 0.59 +/- 0.67. The average tumor weight was 389 mg +/- 64.3. The average tumor size was 300 mm3 +/- 66.23.
  
  
  CONCLUSIONS
  With fluoroscopic guidance, percutaneous implantation of tumor cells in athymic nude mice is simple and effective.
authors.affiliation: Department of Radiology, Louisiana State University Medical Center, New Orleans 70112, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Hamide
authors.forename: Z
authors.initials: Z
authors.lastname: Qian
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Diethelm
authors.forename: N
authors.initials: N
authors.lastname: Skrepnik
authors.forename: W R
authors.initials: WR
authors.lastname: Castaneda-Zuniga
authors.forename: J D
authors.initials: JD
authors.lastname: Hunt
mesh_terms: D000230:Adenocarcinoma / Q000000981:diagnostic imaging* / Q000473:pathology; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000000981:diagnostic imaging* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005471:Fluoroscopy / Q000379:methods*; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured