[The experimental treatment of laryngeal cancer using local draining lymph node lymphocytes].
In order to know whether laryngeal cancer draining lymph node lymphocytes (INL) have antitumor effects in vivo, we transfered IL-2 activated LNL to nude mice with human laryngeal squamous cell carcinoma line PHC3 xenografts, and observed a suppressive effect on tumor growth. It was also found in the epitype changes of LNL by indirect immunofluorencence method the major composition of freshly isolated LNL was CD3+ T cells in which there were more Th/i cells than Ts/c cells, CD4+/CD8+ ratio was 3.30 +/- 0.653. No evidence of lymphocytes multiplication or CD3+, CD4+, CD8+ epitype changes was found after short term IL-2 culture, but we found more HLA-DR+, CD25+ cells which means there was an increase in activated lymphocytes after culture. The experiment suggests that laryngeal cancer draining lymph node lymphocytes have antitumor effects in vivo after IL-2 short term culture, and can be used in clinical immunotherapy.
Wu
Y
Y
First Hospital of Beijing Medical University.
Guo
M
M
D000368:Aged; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000628:therapy*; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000494:pharmacology; D007822:Laryngeal Neoplasms / Q000473:pathology / Q000628:therapy*; D008198:Lymph Nodes / Q000473:pathology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D007376:Interleukin-2