[Genetic control of metabolism of a mutagenic analog of 6-N-hydroxylaminopurine bases in Saccharomyces cerevisiae yeasts].
Yeasts were shown to utilize 6-substituted adenine analogues as a purine source via the reutilization pathway leading to the formation of inosine monophosphate (IMP). This occurs because the ade12 strains with blocked conversion of IMP into adenosine monophosphate (AMP) cannot grow on media containing the above analogues as a sole purine source. Haploid strains with the double mutation ham1 ade2 or ham1 ade5 were also incapable of growing on a medium with 6-N-hydroxylaminopurine (HAP) as a sole purine source. However, in this case, this inability was caused by the occurrence of recessive lethal mutations rather than by a defect in purine reutilization. Yeast adenine aminohydrolase (AAH) can deaminate HAP to hypoxanthine. Adenine aminohydrolase (AAH) was uniformly active both in strains with a mutation in the HAMI gene and in strains wild-type with respect to this trait.
Koz'min
S G
SG
Domkin
V D
VD
Zekhnov
A M
AM
Pavlov
Iu I
IuI
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D005800:Genes, Fungal; D005804:Genes, Lethal; D005808:Genes, Recessive; D009153:Mutagens / Q000378:metabolism*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D009153:Mutagens; C032610:6-N-hydroxylaminopurine; D000225:Adenine