cDNA cloning and characterization of rat C5a anaphylatoxin receptor.
Activation of the complement cascade plays an essential role in the early stages of inflammation. C5a and its receptor are particularly active in anaphylaxis. To determine the pathological roles played by C5a and C5a receptor (C5aR) in rats, we cloned C5aR cDNA and analyzed distribution of its mRNA in various organs including lung from an LPS-stimulated rat. Furthermore, we generated a polyclonal antiserum which specifically recognizes rat C5aR, as confirmed by its specific interaction with cells transfected with rat C5aR cDNA.
Akatsu
H
H
Choju Medical Institute, Fukushimura Hospital, Toyohashi, Aichi, Japan.
Miwa
T
T
Sakurada
C
C
Fukuoka
Y
Y
Ember
J A
JA
Yamamoto
T
T
Hugli
T E
TE
Okada
H
H
D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D005434:Flow Cytometry; D015870:Gene Expression; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D015703:Antigens, CD; D012333:RNA, Messenger; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement