The influence of some compounds commonly used in biochemical studies of mitochondria on the activity of monoamine oxidases.
It was previously described that low concentrations of sodium azide monoamine oxidase (MAO) B assayed by spectrophotometric measurement of benzaldehyde or by hydrogen peroxide accumulation. We failed to confirm this effect using radiometric determination of MAO activity. Tris or dinitrophenol inhibit MAO. The data suggest that some "regulatory effects" depend on the assay of MAO activity.
Zemskova
M A
MA
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
Axenova
L N
LN
Medvedev
A E
AE
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000818:Animals; D001386:Azides / Q000494:pharmacology; D001596:Benzylamines / Q000378:metabolism; D002021:Buffers; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000032:analysis / Q000378:metabolism*; D010627:Phenethylamines / Q000378:metabolism; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D019810:Sodium Azide; D014325:Tromethamine / Q000494:pharmacology
D001386:Azides; D001596:Benzylamines; D002021:Buffers; D004791:Enzyme Inhibitors; D010627:Phenethylamines; D014325:Tromethamine; C029261:phenethylamine; D010649:Phenylalanine; D019810:Sodium Azide; C030796:benzylamine; D008995:Monoamine Oxidase; D019297:2,4-Dinitrophenol