Staining paraffin embedded sections of scald of barley before paraffin removal.
Staining of paraffin embedded sections with periodic acid-Schiff reagent and fast green before paraffin removal resulted in differentiation of barley seed and leaf tissue from fungal structures of Rhynchosporium secalis. Crystal violet, toluidine blue O and antiline blue also successfully stained fungal structures of R. secalis in barley leaf tissues. Staining of embedded sections before paraffin removal allows simple processing of a series of sections, saves time and reduces solvent consumption.
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Agriculture and Agri-Food Canada, Lacombe Research Center, Alberta, Canada.
Burnett
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PA
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D005840:Gentian Violet; D001467:Hordeum / Q000382:microbiology*; D003904:Mitosporic Fungi / Q000737:chemistry*; D016612:Paraffin Embedding / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D012639:Seeds / Q000382:microbiology; D013194:Staining and Labeling / Q000379:methods; D014048:Tolonium Chloride
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D014048:Tolonium Chloride; C017006:aniline blue; D005840:Gentian Violet