Influence of combined use of selenious acid and SH compounds in parenteral preparations.
The influence of the combined use of selenious acid and SH compounds (glutathione (GSH) and cysteine (Cys), or ascorbic acid (Asc)) on cultured venous vascular cells was investigated experimentally. When cultured human umbilical venous vascular endothelial cells were exposed to 10 microM of selenious acid combined with 0.5 mM-GSH or 0.5 mM-Cys, the release rates of [3H]-adenine and lactate dehydrogenase (LDH) from cells into the medium increased significantly as compared with after exposure to selenious acid alone, and damage to the vascular endothelial cells was found to be intensified. Addition of 1 microM of selenious acid simultaneously with 0.5 mM-GSH or 0.5 mM-Cys showed no differences in toxicity for the vascular endothelial cells as compared with the addition of selenious acid alone. On the other hand, simultaneous exposure to 10 microM of selenious acid and 1 mM-Asc induced no significant differences in the release rates of [3H]-adenine and LDH, and no damage was observed to the vascular endothelial cells. These results suggest that simultaneous addition of selenious acid together with GSH or Cys, which have the SH-group, may cause damage to the vascular endothelial cells. Therefore careful attention is warranted in total parenteral nutrition (TPN).
Terada
A
A
Department of Pharmacy, St. Marianna University School of Medicine, Kawasaki, Japan.
Yoshida
M
M
Nakada
M
M
Nakada
K
K
Yamate
N
N
Kobayashi
T
T
Yoshida
K
K
D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D002478:Cells, Cultured; D003545:Cysteine / Q000008:administration & dosage* / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005978:Glutathione / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D010289:Parenteral Nutrition, Total; D020887:Selenious Acid; D018036:Selenium Compounds / Q000008:administration & dosage* / Q000494:pharmacology
D018036:Selenium Compounds; D020887:Selenious Acid; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid