Structural characterization of three aldobiuronic acids derived from the capsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus.
This study deals with the chemical characterization of a capsular polysaccharide (CPS) produced by a thermal biomass formed by the cyanobacterium Mastigocladus laminosus. Acid hydrolysis performed on the purified polysaccharide has led to the isolation of several acid-resistant oligosaccharides. Two of them have already been reported and assigned as: alpha - GlcA - (1 --> 2) - alpha - GalA - (1 --> 2) - Man. and alpha - GlcA - (1 --> 2) - alpha - GalA - (1 --> 2) - beta - Man - (1 --> 4) - beta - Gal(1 --> 2) - Rha. In this report, results on the isolation and partial purification of three supplementary oligosaccharidic units are presented. Gas chromatography-mass spectrometry (GC-MS) and H-nuclear magnetic resonance (NMR) spectroscopy investigations allowed them to be assigned as three aldobiuronic acids with the following structures: alpha - GlcA - (1 --> 3) - Gal alpha - GlcA - (1 --> 3) - Fuc alpha - GalA - (1 --> 3) - Fuc.
Gloaguen
V
V
Laboratoire de Biologie Cellulaire Végétale et Valorisation des Espèces Ligneuses, Université de Limoges, France. Ibcvel@unilim.fr
Plancke
Y
Y
Strecker
G
G
Vebret
L
L
Hoffmann
L
L
Morvan
H
H
D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid / Q000379:methods; D000458:Cyanobacteria / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism*
D011135:Polysaccharides, Bacterial