Release of mixed linkage (1-->3),(1-->4) beta-D-glucans from barley by protease activity and effects on ileal effluent.
The behaviour of barley beta-glucans in the upper gut has been assessed using ileal effluents recovered from a barley-based test meal. Results have been compared to in vitro treatments used to extract beta-glucans. In vitro, exposure to endogenous proteases led to a solubilisation of beta-glucan, ranging from 28% in the untreated to 83% following NSP isolation. In ileal effluent 60% of the beta-glucan was solubilised, similar to the in vitro treatment. However, the viscosity of the ileal effluent was low, comparable to a mucin standard. Although beta-glucan can be solubilised in the upper gut its viscosity would appear to have only a limited potential to affect nutrient bioavailability.
Robertson
J A
JA
Department of Biochemistry, Institute of Food Research, Norwich Research Park, Colney, UK.
Majsak-Newman
G
G
Ring
S G
SG
D002236:Carbohydrate Conformation; D004435:Eating; D010450:Endopeptidases / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry / Q000378:metabolism*; D001467:Hordeum / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007081:Ileostomy; D007082:Ileum / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D014783:Viscosity
D005936:Glucans; D011134:Polysaccharides; D010450:Endopeptidases