[Augmentation of liver immune functions by intrasplenic transplantation of IL-2 gene-modified hepatocytes].
OBJECTIVE
To investigate the effects of intrasplenically transplanted, IL-2 gene-modified hepatocytes on the liver immune response and anti-tumor activity.


METHODS
Murine fetal hepatocytes BNL CL. 2 were transfected with mIL-2 gene in vitro, and intrasplenically transplanted into syngeneic mice (2 x 10(6)/mouse). After two weeks, the Kupffer cells were isolated and studied for cytotoxicity, TNF and NO production, and Ia expresson. The therapeutic effects on the mice with the metastatic liver carcinoma were also observed.


RESULTS
The Kupffer's cytotoxicity to L1210, Yac-1, P815 and H22 target cells, and their production of TNF and NO were greatly augmented, even when freshly prepared. Their Ia expression also increased. When treated with IL-2 gene-modified hepatocytes, the metastatic liver carcinoma-bearing mice survived for a longer time.


CONCLUSIONS
Intrasplenic transplantation of IL-2 gene-modified hepatocytes could effectively activate the liver immune response and exert potent therapeutic effects on liver carcinoma-bearing host.
Cao
X
X
Department of Immunology, Second Military Medical University, Shanghai.
Zhang
W
W
Wang
J
J
D000818:Animals; D015316:Genetic Therapy; D007376:Interleukin-2 / Q000235:genetics*; D007728:Kupffer Cells / Q000276:immunology / Q000637:transplantation*; D008099:Liver / Q000166:cytology / Q000276:immunology*; D008113:Liver Neoplasms / Q000556:secondary / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D011897:Random Allocation; D013154:Spleen / Q000601:surgery; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide