A mucosal irritancy test device for intraoral use in dogs.
The objective of this investigation was to conduct, in dogs, a 6-hour acute mucosal irritation study of a new bioerodible treatment. The main problem was to retain the test drug in situ without extraneous irritation from the retention device. A buccal cup was machined from acrylic with a chamber capacity of 0.025 ml and a flange that could be ligated to a tooth. Ten female retired breeder beagles, 7 to 8 years old, with naturally occurring moderate periodontitis were examined and the upper canine teeth scaled to remove plaque and calculus. One week later the buccal cups were placed at the gingival margin of the upper canine teeth and fixed in place with wire ligature passing through holes in the flange and around the tooth, engaging shallow nicks made in the enamel near the gingival margin so the wire would not slide up and down. The buccal cup was further stabilized with glass ionomer cement placed on the crown and over the outer surface of the cup. Buccal cups were loaded with test, placebo, or no ointment by technicians in a scheme to which examiners were blind. Hourly examinations were made, and after 6 hours the cups were removed and gingival mucosa scored for irritation by a previously described method. All dogs tolerated the test and retained the cups for 6 hours. There was no mucosal irritation from the empty cups. We conclude that this new device can be used successfully for testing new agents for short-term mucosal irritancy.
Wirthlin
M R
MR
Department of Stomatology, School of Dentistry, University of California, San Francisco 94143-0762, USA.
Armitage
G C
GC
Rao
S
S
Fritzinger
B
B
Phillips
S
S
Heller
J
J
D000180:Acrylic Resins; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000009:adverse effects; D002484:Cementation; D003481:Cuspid; D003728:Dental Calculus / Q000628:therapy; D003743:Dental Enamel; D003773:Dental Plaque / Q000628:therapy; D012534:Dental Scaling; D004285:Dogs; D016503:Drug Delivery Systems / Q000295:instrumentation*; D004867:Equipment Design; D005260:Female; D005881:Gingiva / Q000187:drug effects*; D005899:Glass Ionomer Cements; D007509:Irritants / Q000008:administration & dosage / Q000009:adverse effects*; D008026:Ligation; D009061:Mouth Mucosa / Q000187:drug effects*; D009824:Ointments; D010518:Periodontitis / Q000503:physiopathology / Q000628:therapy; D010919:Placebos; D016037:Single-Blind Method; D013754:Tetracyclines; D013997:Time Factors
D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D005899:Glass Ionomer Cements; D007509:Irritants; D009824:Ointments; D010919:Placebos; D013754:Tetracyclines