Inhibition of tyrosine hydroxylase by bulbocapnine.
The inhibitory effects of bulbocapnine, an aporphine isoquinoline alkaloid, on bovine adrenal tyrosine hydroxylase (TH) were investigated. Bulbocapnine had an inhibitory effect on the enzyme (43.6% inhibition at concentration of 200 microM). Bulbocapnine exhibited uncompetitive inhibition on bovine adrenal TH with a substrate L-tyrosine. The Ki value was found to be 0.20 mM.
Zhang
Y H
YH
Shin
J S
JS
Lee
S S
SS
Kim
S H
SH
Lee
M K
MK
D000311:Adrenal Glands / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D001060:Aporphines / Q000494:pharmacology*; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D016716:PC12 Cells; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors*
D001060:Aporphines; D004791:Enzyme Inhibitors; D014446:Tyrosine 3-Monooxygenase; C083807:bulbocapnine