Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range.
We report three-dimensional (3D) microscopy with nearly isotropic resolution in the lambda/5-lambda/10 range. Our approach combines 4PI-confocal two-photon fluorescence microscopy with image restoration. The 3D resolution is demonstrated with densely clustered beads as well as with F-actin fibers in mouse fibroblast cells. A comparison with unrestored two-photon confocal images reveals a total reduction of the uncertainty volume up to a factor of 15.
Hell
S W
SW
Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany. shell@gwdg.de
Schrader
M
M
van der Voort
H T
HT
D000818:Animals; D051379:Mice; D008856:Microscopy, Fluorescence