High dietary omega-6 polyunsaturated fatty acids drastically increase the formation of etheno-DNA base adducts in white blood cells of female subjects.
Lipid peroxidation generates reactive aldehydes such as trans-4-hydroxy-2-nonenal and malonaldehyde, which form promutagenic exocyclic DNA adducts in human cells and may contribute to diet-related cancers. Using ultrasensitive detection methods, analysis of WBC DNA from volunteers in a dietary study revealed that high intake of omega-6 polyunsaturated fatty acids increased malonaldehyde-derived adducts in male and female subjects. In contrast, etheno adducts (1,N6-ethenodeoxyadenosine; 3,N4-ethenodeoxycytidine) were not elevated in males but were, on average, 40 times higher in females, displaying a huge intersubject variation in lipid peroxidation-derived DNA damage. Exocyclic DNA adducts are promising biomarkers for examining the hypothesis of possible links between increased intake of dietary omega-6 polyunsaturated fatty acids, DNA damage, and elevated cancer risk for breast, colon, and prostate.
Nair
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J
Division of Toxicology and Cancer Risk Factors, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Vaca
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Velic
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Mutanen
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Valsta
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Bartsch
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D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D018736:DNA Adducts / Q000097:blood*; D003839:Deoxyadenosines / Q000097:blood*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000097:blood; D004042:Dietary Fats, Unsaturated / Q000009:adverse effects*; D005229:Fatty Acids, Monounsaturated; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000009:adverse effects*; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D007962:Leukocytes; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000209:etiology*; D010938:Plant Oils / Q000008:administration & dosage; D000074262:Rapeseed Oil; D012307:Risk Factors; D012737:Sex Factors; D013329:Structure-Activity Relationship; D000074242:Sunflower Oil
D014408:Biomarkers, Tumor; D018736:DNA Adducts; D003839:Deoxyadenosines; D004042:Dietary Fats, Unsaturated; D005229:Fatty Acids, Monounsaturated; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; D000074242:Sunflower Oil; D003841:Deoxycytidine; C032221:1,N(6)-ethenodeoxyadenosine; C032222:3,N(4)-ethenodeoxycytidine