Fusion of phospholipid vesicles induced by the ribosome inactivating protein saporin.
The single chain ribosome-inactivating protein Saporin-S6 (SO-6) induces the fusion of acid phospholipid vesicles. The extent of fusion was measured by resonance energy transfer assay between the N-(7-nitro-2-1,3-benzoxadiazol-4-yl)-dimyristoylphosphatidyl lithanolamine (NBD-PE)(donor) and N-(lissamine rhodamine B sulphonyl)-diacylphoshaidylethanolamine (Rh-PE) (acceptor) incorporated in the vesicle. The saturated lipid/protein molar ratio is approx. 100:1. The time course of fusion of vesicles induced by the protein showed that the process was completed within 10 minutes, and the size of the particles in the medium was enlarged which conforms the occurrence of the fusion occurring. The fusion is temperature dependent and the liquid-crystalline state lipid is more apt to fuse than the gel phase lipid. The effect of SO-6 is also dependent on ionic strength and pH, high salt concentration and basic pH may abolish fusion, which suggests that both electrostatic and hydrophobic components may be involved in the process.
Liu
G
G
Department of Molecular Biology, Jilin University, Changchun, China.
Hao
Q
Q
Zhang
Y
Y
Gao
G
G
Yan
G
G
Yao
Q
Q
Li
Q
Q
D006863:Hydrogen-Ion Concentration; D000922:Immunotoxins; D008561:Membrane Fusion / Q000187:drug effects*; D008567:Membranes, Artificial; D009699:N-Glycosyl Hydrolases; D010743:Phospholipids / Q000737:chemistry*; D010940:Plant Proteins / Q000494:pharmacology*; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013696:Temperature
D000922:Immunotoxins; D008567:Membranes, Artificial; D010743:Phospholipids; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins