Blackgram mottle virus RNA and its in vitro translation.
In vitro translation of blackgram mottle virus RNA in rabbit reticulocyte lysate resulted in synthesis of five major virus specific polypeptides with mol wt 90,000(p90), 82,000(p82), 42,000(p42), 39,000(p39) and 32,000(p32), respectively. The polypeptide p39 was identified as coat protein based on its electrophoretic mobility and immunoprecipitation with BMoV-antiserum.
Balasubrahmanyam
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Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India.
Kapoor
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HC
Varma
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D000818:Animals; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012328:RNA Viruses / Q000235:genetics* / Q000472:pathogenicity; D012367:RNA, Viral / Q000235:genetics*; D011817:Rabbits; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins