Hepatic damage influences the decay of nitroxide radicals in mice--an in vivo ESR study.
To determine the role of the liver in the elimination of free radicals from the body, the clearance rate (K) of nitroxide radicals (Tempol) at the hepatic domain was compared with that at the pelvic domain of live mice, using L-band ESR spectroscopy. The reduction of Tempol in biopsy specimens (liver tissue and femoral muscle) and blood obtained from Tempol-treated mice was also monitored using X-band ESR spectroscopy. Results indicated that the reduction of nitroxide radicals was delayed in both the liver and peripheral tissues when the liver was damaged. The decrease in both blood supply and reductants in the damaged liver might be involved in delaying the reduction in the whole body, because the liver can reduce the radicals supplied via the blood from the peripheral tissues, and the reductants such as reduced, glutathione in the peripheral tissues are supplied from the liver.
Inaba
K
K
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Nakashima
T
T
Shima
T
T
Mitsuyoshi
H
H
Sakamoto
Y
Y
Okanoue
T
T
Kashima
K
K
Hashiba
M
M
Nishikawa
H
H
Watari
H
H
D000818:Animals; D000975:Antioxidants / Q000493:pharmacokinetics; D002251:Carbon Tetrachloride / Q000494:pharmacology; D003497:Cyclic N-Oxides / Q000097:blood / Q000493:pharmacokinetics; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009589:Nitrogen Oxides / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis; D010101:Oxygen Consumption; D010388:Pelvis / Q000502:physiology; D013113:Spin Labels
D000975:Antioxidants; D003497:Cyclic N-Oxides; D005609:Free Radicals; D009589:Nitrogen Oxides; D013113:Spin Labels; D002251:Carbon Tetrachloride; D005978:Glutathione; D010100:Oxygen; C001803:tempol