title: A chronic model to simultaneously measure intracranial pressure, cerebral blood flow, and study the pial microvasculature.
abstract: In an effort to study changes in cerebral blood flow (CBF), intracranial pressure (ICP) and intracranial compliance (ICC) simultaneously, we have developed a chronic model in rats using a pial window crown with two ports. This model can also be used to study vasoreactivity of pial vessels. Female Sprague-Dawley rats weighing between 225-250 g underwent placement of cranial chamber with dual ports under pentobarbital anesthesia. To test the utility of this technique 45 groups of rats were studied. Group 1 consisted of control animals. Group 2 consisted of rats undergoing 15 min of global cerebral ischemia. Rats in group 3 were evaluated for changes in vessel diameter and ICP after adenosine injection. In group 4 leukocyte/endothelial interactions were evaluated. These groups demonstrate the ability of this model to monitor CBF, ICP, ICC and pial vessel architecture in chronic rat experiments.
authors.affiliation: Temple University Health Sciences Center, Department of Neurosurgery, Temple University Hospital, Philadelphia, PA 19140, USA.
authors.forename: J
authors.initials: J
authors.lastname: Jallo
authors.forename: R
authors.initials: R
authors.lastname: Saetzler
authors.forename: C
authors.initials: C
authors.lastname: Mishke
authors.forename: W F
authors.initials: WF
authors.lastname: Young
authors.forename: U
authors.initials: U
authors.lastname: Vasthare
authors.forename: R F
authors.initials: RF
authors.lastname: Tuma
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology; D002550:Cerebral Veins / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D005260:Female; D007427:Intracranial Pressure / Q000502:physiology*; D008833:Microcirculation / Q000502:physiology; D008954:Models, Biological; D051381:Rats; D017207:Rats, Sprague-Dawley; D013238:Stereotaxic Techniques