Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide.
Neuropeptide Y and nitric oxide (NO) synthase are colocalized in nervous tissues. We tested the hypothesis whether or not NO might be involved in the release of neuropeptide Y. Neuropeptide Y concentration in the supernatant of PC12 rat pheochromocytoma cells, shown to express NO synthase I by immunohistochemistry, rose threefold in a time- and dose-dependent manner following sodiumnitroprusside and 3-morpholinosydnonimine (SIN-1) incubation. Neuropeptide Y mRNA expression was induced by NO-donors as a function of incubation-time. Neuropeptide Y production rose fivefold with zaprinast, an inhibitor of the phosphodiesterase V and threefold with nerve growth factor (NGF). Combined application of zaprinast and NGF did not further increase neuropeptide Y production while combination of zaprinast and sodiumnitroprusside potentiated the NO effect on neuropeptide Y release. The data suggest that NO regulates neuropeptide Y secretion of PC12 pheochromocytoma cells on the mRNA level.
Dötsch
J
J
Department of Pediatrics, Justus-Liebig-Universität Giessen, Germany.
Hänze
J
J
Dittrich
K
K
Demirakça
S
S
Haberberger
R
R
Rascher
W
W
D000818:Animals; D015152:Blotting, Northern; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007150:Immunohistochemistry; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology; D009478:Neuropeptide Y / Q000378:metabolism*; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000378:metabolism*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011688:Purinones / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D011863:Radioimmunoassay; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D009414:Nerve Growth Factors; D009478:Neuropeptide Y; D010726:Phosphodiesterase Inhibitors; D011688:Purinones; D009599:Nitroprusside; D009569:Nitric Oxide; C002385:linsidomine; D012313:RNA; D008981:Molsidomine; D019001:Nitric Oxide Synthase; D000254:Sodium-Potassium-Exchanging ATPase; C011145:zaprinast