Allelic variation in Streptococcus pneumoniae autolysin (N-acetyl muramoyl-L-alanine amidase).
The lytA gene encoding the autolysin of Streptococcus pneumoniae may be a virulence determinant. Single-strand conformational polymorphism analysis demonstrated heterogenicity throughout the gene in clinical isolates and strains from the clonal serotypes 7 and 14. Sequence analysis of part of the choline-binding domain showed that in two isolates four amino acid substitutions occurred.
Gillespie
S H
SH
Department of Medical Microbiology, The Royal Free Hospital School of Medicine, London, United Kingdom. stepheng@rfhsm.ac.uk
McHugh
T D
TD
Ayres
H
H
Dickens
A
A
Efstratiou
A
A
Whiting
G C
GC
D000483:Alleles; D014644:Genetic Variation; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D013296:Streptococcus pneumoniae / Q000235:genetics*
C076332:EJL amidase; D009238:N-Acetylmuramoyl-L-alanine Amidase