Effect of certain nutrients on the production of dextransucrase from Leuconostoc mesenteroides NRRL B-512F.
The effects of certain nutrients on dextransucrase (sucrose: 1,6-alpha-d-glucan 6-alpha-d-glucosyltransferase EC 2.4.1.5) production from Leuconostoc mesenteroides NRRL B-512F were studied. An increase in concentration of sucrose to 4% in the enzyme production medium resulted in the increase of activity of dextransucrase. Higher enzyme yields were obtained at low yeast extract and high phosphate concentrations. The presence of peptone and beef extract in the medium in addition to 2% yeast extract resulted in an enhanced production of dextransucrase. The enzyme activity increased by 30% by both peptone and beef extract. Addition of Tween 80 to the medium enhanced the production of enzyme and the activity was increased by 25%. Magnesium ions stimulated the activity marginally. Sodium fluoride enhanced the activity of dextransucrase by 25%.
Goyal
A
A
Department of Chemistry, Indian Institute of Technology Kanpur, India.
Katiyar
S S
SS
D003470:Culture Media; D005964:Glucosyltransferases / Q000096:biosynthesis*; D007934:Leuconostoc / Q000187:drug effects / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D015002:Yeast, Dried / Q000494:pharmacology
D003470:Culture Media; D010710:Phosphates; D011136:Polysorbates; D013501:Surface-Active Agents; D013395:Sucrose; D012969:Sodium Fluoride; D005964:Glucosyltransferases; C020025:dextransucrase; D008274:Magnesium