Functional cloning of a cDNA encoding Mei2-like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant.
To isolate Arabidopsis cDNAs that encode signal transducers and components involved in the regulation of meiosis, a trans-complementation analysis was performed using a Schizosaccharomyces pombe meiosis-defective mutant in which the genes for pheromone receptors were disabled. One cDNA obtained in this screening encodes a polypeptide, named AML1, that shows significant similarity to S. pombe Mei2 protein and has three putative RNA-recognition motifs like as Mei2. Mei2 is involved in the regulation of meiosis in fission yeast. Northern blot analysis showed that the AML1 gene is expressed in each organ. The possible functions of AML1 are discussed.
Hirayama
T
T
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan. takashih@sas.upenn.edu
Ishida
C
C
Kuromori
T
T
Obata
S
S
Shimoda
C
C
Yamamoto
M
M
Shinozaki
K
K
Ohto
C
C
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D002628:Chemoreceptor Cells / Q000378:metabolism*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008540:Meiosis; D008969:Molecular Sequence Data; D009154:Mutation; D009698:Nucleoproteins; D010940:Plant Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid
C108559:AML1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004271:DNA, Fungal; D005656:Fungal Proteins; D009698:Nucleoproteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface; D029702:Schizosaccharomyces pombe Proteins; C088896:mei2 protein, S pombe