Glycosaminoglycan supplementation promotes nerve regeneration and muscle reinnervation.
This study shows that treatment of rats with exogenous glycosaminoglycans stimulates peripheral nerve regeneration, increases the abundance of mRNAs for myelin proteins and promotes muscle reinnervation. After the sciatic nerve had been crushed the number of regenerating axons in the distal stump was markedly and highly significantly increased by glycosaminoglycan treatment throughout the experimental period. The increased number of axons was correlated with increased axon and fibre (axon+myelin) diameter. The abundance of mRNAs for P0 protein and myelin basic protein of regenerating nerves was also affected by treatment with glycosaminoglycans. The increase in mRNA was also observed in the contralateral unlesioned nerve. Such a phenomenon did not occur in saline-treated rats. Glycosaminoglycan treatment markedly increased the number of muscle fibres reinnervated and accelerated the restoration of muscle twitch tension elicited by nerve stimulation. The effect was particularly evident during the early stages (16 and 21 days after nerve crush) of muscle reinnervation.
Gorio
A
A
Laboratory for Research on Pharmacology of Neurodegenerative Disorders, Department of Pharmacology, Chemotherapy and Medical Toxicology, Milan, Italy.
Lesma
E
E
Vergani
L
L
Di Giulio
A M
AM
D000818:Animals; D006025:Glycosaminoglycans / Q000494:pharmacology*; D008297:Male; D009132:Muscles / Q000294:innervation*; D009185:Myelin Proteins / Q000235:genetics; D009416:Nerve Regeneration / Q000187:drug effects*; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries
D006025:Glycosaminoglycans; D009185:Myelin Proteins; D012333:RNA, Messenger