MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13.
A human genomic DNA fragment containing the 5' region of MCM4, a gene encoding replication protein MCM4/Cdc21 was isolated. At a distance of about 800 base pairs upstream of MCM4, the fragment was shown to also contain the 5' end of PRKDC, a gene encoding the catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs). The genomic DNA fragment was used for hybridization to human metaphase chromosome spreads. The cytogenetic map location of the two closely adjacent genes was determined to be 8q12-->q13.
Ladenburger
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Division of Biology, Universität Konstanz, Germany.
Fackelmayer
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Hameister
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Knippers
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D018797:Cell Cycle Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics* / Q000648:ultrastructure; D004261:DNA Replication / Q000235:genetics; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D064148:Minichromosome Maintenance Complex Component 4; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D015183:Restriction Mapping; D029702:Schizosaccharomyces pombe Proteins
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases; D064148:Minichromosome Maintenance Complex Component 4; C078490:mcm4 protein, S pombe