title: Diamino acid derivatives of porphyrins penetrate into yeast cells, induce photodamage, but have no mutagenic effect.
abstract: The yeast Saccharomyces cerevisiae was used as a model eukaryotic organism to study the uptake of diamino acid derivatives of porphyrins and their phototoxicity with particular emphasis on possible mutagenic effects. The water-soluble hematoporphyrin derivatives diarginate (HpD[Arg]2) and 1-arginin di(N-amino acid)-protoporphyrinate used in this study are effective photosensitizers in tumor photodynamic therapy. Depending on the amino acid substituent, the porphyrin derivatives differ in their affinity for yeast cells. It is shown that HpD(Arg)2 and PP(Met)2 (Arg)2 penetrate into the yeast cell and are metabolized. Both compounds sensitize yeast cells to photodamage but have no mutagenic effect on nuclear or mitochondrial genomes.
authors.affiliation: Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland. teresa@ibbrain.ibb.waw.pl
authors.forename: T
authors.initials: T
authors.lastname: Zoładek
authors.forename: B N
authors.initials: BN
authors.lastname: Nguyen
authors.forename: I
authors.initials: I
authors.lastname: Jagiełło
authors.forename: A
authors.initials: A
authors.lastname: Graczyk
authors.forename: J
authors.initials: J
authors.lastname: Rytka
mesh_terms: D000599:Amino Acids, Diamino / Q000378:metabolism* / Q000494:pharmacology; D006415:Hematoporphyrins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D016296:Mutagenesis; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011524:Protoporphyrins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D000599:Amino Acids, Diamino; D006415:Hematoporphyrins; D017319:Photosensitizing Agents; D011524:Protoporphyrins; C028025:protoporphyrin IX