Identification of bovine rotaviruses in Venezuela: antigenic and molecular characterization of a bovine rotavirus strain.
Two serotypes of bovine group A rotaviruses were demonstrated by enzyme-linked immunoassay (ELISA) and polyacrylamide gel electrophoresis (PAGE) in 20 of 171 faecal samples collected from diarrhoeic calves in two dairy farms in Venezuela. By serotyping ELISA using G and P serotype-specific monoclonal antibodies, bovine rotaviruses (BRV) circulating on one farm were identified as serotype G6, while BRVs circulating on the other farm were identified as serotype G10. Only one BRV (033) could be successfully isolated in MA104 cells, and the nucleotide sequences of the VP7 and the VP8* trypsin-cleavage product of the VP4 were determined. Cross-neutralization tests and comparative sequence analysis showed that BRV 033 belonged to serotype G6 and genotype P1. This is the first report of BRVs identified in Venezuela.
Ciarlet
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Laboratorio Biología de Virus, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Piña
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CI
García
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O
Liprandi
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F
D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D008969:Molecular Sequence Data; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D014687:Venezuela
D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus