title: Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli.
abstract: Escherichia coli sigma54-dependent phage shock protein operon (pspA to -E) transcription is under the control of PspF, a constitutively active activator. Sigma70-dependent transcription of pspF is under autogenous control by wild-type PspF but not by a DNA-binding mutant, PspF deltaHTH. Negative autoregulation of PspF is continual and not affected by stimuli, like f1 pIV, that induce the pspA to -E operon. PspF production is independent of PspA (the negative regulator of the pspA to -E operon) and of PspB and -C (positive regulators).
authors.affiliation: Laboratory of Genetics, The Rockefeller University, New York, New York 10021, USA.
authors.forename: G
authors.initials: G
authors.lastname: Jovanovic
authors.forename: J
authors.initials: J
authors.lastname: Dworkin
authors.forename: P
authors.initials: P
authors.lastname: Model
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D009876:Operon; D012045:Regulatory Sequences, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; C098954:PspF protein, E coli; D015534:Trans-Activators; C069816:phage shock protein, Bacteria