Lif, the lysostaphin immunity factor, complements FemB in staphylococcal peptidoglycan interpeptide bridge formation.
The formation of the Staphylococcus aureus peptidoglycan pentaglycine interpeptide chain needs FemA and FemB for the incorporation of glycines Gly2-Gly3, and Gly4-Gly5, respectively. The lysostaphin immunity factor Lif was able to complement FemB, as could be shown by serine incorporation and by an increase in lysostaphin resistance in the wild-type as well as in a femB mutant. However, Lif could not substitute for FemA in femA or in femAB-null mutants. Methicillin resistance, which is dependent on functional FemA and FemB, was not complemented by Lif, suggesting that serine-substituted side chains are a lesser substrate for penicillin-binding protein PBP2' in methicillin resistance.
Tschierske
M
M
Institute of Medical Microbiology, University of Zürich, Switzerland.
Ehlert
K
K
Strandén
A M
AM
Berger-Bächi
B
B
D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002352:Carrier Proteins; D002473:Cell Wall / Q000737:chemistry; D005816:Genetic Complementation Test; D005998:Glycine / Q000032:analysis / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism; D008248:Lysostaphin; D016106:Methicillin Resistance; D009097:Multienzyme Complexes / Q000378:metabolism; D002267:Muramoylpentapeptide Carboxypeptidase; D009154:Mutation; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan / Q000096:biosynthesis / Q000737:chemistry*; D010458:Peptidyl Transferases / Q000378:metabolism; D012694:Serine / Q000032:analysis; D013211:Staphylococcus aureus / Q000378:metabolism*
D000596:Amino Acids; D001426:Bacterial Proteins; D002352:Carrier Proteins; C096686:FemA protein, Bacteria; C102928:FemB protein, Staphylococcus epidermidis; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; D012694:Serine; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D008248:Lysostaphin; D005998:Glycine