title: Isolation of verotoxin-producing Escherichia coli O-rough:K1:H7 from two patients with traveler's diarrhea.
abstract: Two Escherichia coli O-rough:K1:H7 strains producing verotoxin 1 that were isolated from stool samples of two travelers with diarrhea who consulted our clinic after trips to the Indian Subcontinent and Central America were characterized. Both strains were sorbitol negative, the same phenotype presented by E. coli O157:H7, but in contrast they were beta-glucuronidase positive. Low-frequency restriction analysis of chromosomal DNA and pulsed-field gel electrophoresis and repetitive extragenic palindrome-PCR showed that both strains were epidemiologically related. The illness was self-limited in both cases but involved long-duration, watery diarrhea (10 to 50 days) accompanied by abdominal cramps and flatulence. This serotype should be taken into account as a possible cause of traveler's diarrhea.
authors.affiliation: Department of Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Spain. jvila@microclinic.med.ub.es
authors.forename: J
authors.initials: J
authors.lastname: Vila
authors.forename: M
authors.initials: M
authors.lastname: Vargas
authors.forename: J
authors.initials: J
authors.lastname: Ruiz
authors.forename: F
authors.initials: F
authors.lastname: Gallardo
authors.forename: M T
authors.initials: MT
authors.lastname: Jimenez de Anta
authors.forename: J
authors.initials: J
authors.lastname: Gascón
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001427:Bacterial Toxins / Q000378:metabolism*; D001431:Bacteriological Techniques; D002489:Central America / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D019081:O Antigens / Q000276:immunology / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D022622:Shiga Toxin 1; D014195:Travel
chemical_list: D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D019081:O Antigens; D022622:Shiga Toxin 1