Evidence for mitochondrial metabolism of 7,12-dimethylbenz(a)anthracene in porcine ovaries: comparison with microsomal metabolism.
7,12-dimethylbenz(a)anthracene (DMBA) causes necrosis in endocrine organs. DMBA metabolism in follicles and corpora lutea from porcine ovaries was demonstrated not only in the microsomal but also in the mitochondrial fraction, in contrast to what has been found in the rat ovary. Maximal activities were present in these fractions of the corpus luteum, with specific activities of 5.9 and 2.2 pmol/min x mg protein, respectively. DMBA metabolism in mitoplasts, i.e. mitochondrial inner membranes, proved to be more than 10-fold higher than the corresponding activity in the mitochondrial fraction. The purities of the subcellular fractions were assessed by measurements of marker enzymes. 17-42% of the mitochondrial DMBA metabolism was concluded to be due to microsomal contamination. In the mitoplast fraction such contamination was only 0.18-2.8%. Ellipticine and alpha-naphthoflavone reduced the metabolism of DMBA in the luteal microsomal fraction by 95 and 77%, respectively. In mitochondria the inhibition by these agents was 63 and 30%, respectively. Indomethacine and estradiol decreased microsomal DMBA metabolism by 53 and 52%, respectively. In mitochondria the inhibition was 52 and 23%, respectively. None of these inhibitors affected the DMBA metabolism by the mitoplast fraction.
Eliasson
M
M
Department of Biochemistry, Stockholm University, Sweden.
Brock
S
S
Bengtsson Ahlberg
M
M
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000493:pharmacokinetics*; D000818:Animals; D001711:Biotransformation; D003338:Corpus Luteum / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005260:Female; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D008995:Monoamine Oxidase / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D006080:Ovarian Follicle / Q000201:enzymology; D010053:Ovary / Q000201:enzymology* / Q000378:metabolism* / Q000648:ultrastructure; D013267:Stilbenes / Q000493:pharmacokinetics; D013552:Swine
D013267:Stilbenes; D015127:9,10-Dimethyl-1,2-benzanthracene; D003577:Cytochrome P-450 Enzyme System; D008995:Monoamine Oxidase; D009247:NADH, NADPH Oxidoreductases; C025906:stilbene oxide