title: The protein product of the het-s heterokaryon incompatibility gene of the fungus Podospora anserina behaves as a prion analog.
abstract: The het-s locus of Podospora anserina is a heterokaryon incompatibility locus. The coexpression of the antagonistic het-s and het-S alleles triggers a lethal reaction that prevents the formation of viable heterokaryons. Strains that contain the het-s allele can display two different phenotypes, [Het-s] or [Het-s*], according to their reactivity in incompatibility. The detection in these phenotypically distinct strains of a protein expressed from the het-s gene indicates that the difference in reactivity depends on a posttranslational difference between two forms of the polypeptide encoded by the het-s gene. This posttranslational modification does not affect the electrophoretic mobility of the protein in SDS/PAGE. Several results suggest a similarity of behavior between the protein encoded by the het-s gene and prions. The [Het-s] character can propagate in [Het-s*] strains as an infectious agent, producing a [Het-s*] --> [Het-s] transition, independently of protein synthesis. Expression of the [Het-s] character requires a functional het-s gene. The protein present in [Het-s] strains is more resistant to proteinase K than that present in [Het-s*] mycelium. Furthermore, overexpression of the het-s gene increases the frequency of the transition from [Het-s*] to [Het-s]. We propose that this transition is the consequence of a self-propagating conformational modification of the protein mediated by the formation of complexes between the two different forms of the polypeptide.
authors.affiliation: Laboratoire de Génétique Moléculaire des Champignons Filamenteux, Institut de Biochimie et Génétique Cellulaires, Centre National de la Recherche Scientifique Unité Propre de Recherche 9026, 33077 Bordeaux cedex, France.
authors.forename: V
authors.initials: V
authors.lastname: Coustou
authors.forename: C
authors.initials: C
authors.lastname: Deleu
authors.forename: S
authors.initials: S
authors.lastname: Saupe
authors.forename: J
authors.initials: J
authors.lastname: Begueret
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D011328:Prions / Q000235:genetics*; D011499:Protein Processing, Post-Translational / Q000235:genetics
chemical_list: D005656:Fungal Proteins; C083891:HET-S protein, Podospora anserina; D011328:Prions