Cloning of the antigen 85A from Mycobacterium gordonae and its use for the specific PCR identification of these mycobacteria.
The complete nucleotide sequence of 85A antigen of Mycobacterium gordonae was determined. This gene encodes 339 amino acids, including 43 amino acids for the signal peptide, followed by a mature protein of 296 amino acids. A polymerase chain reaction (PCR) assay for the rapid detection of M. gordonae DNA using two pairs of oligonucleotide primers, derived from our sequence, is described. This one-step PCR has been used successfully to amplify 38 strains of M. gordonae. Conversely, the primers did not amplify DNA from any of the 25 mycobacterial species tested. The results suggest that this PCR assay could be a good alternative to existing commercial assays for the specific identification of M. gordonae on early culture on solid medium or on early BACTEC broth culture.
Dumonceaux
M
M
Department of Virology, Institut Pasteur, 642 rue Engeland, Brussels, B-1180, Belgium.
Fauville Dufaux
M
M
Ooms
J
J
De Wit
L
L
Sonck
P
P
Content
J
J
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D000942:Antigens, Bacterial; D017931:DNA Primers; C108572:antigen 85A, Mycobacterium gordonae