DNA transfer into vascular smooth muscle using fusigenic Sendai virus (HVJ)-liposomes.
Manipulation of the genetic machinery of cells both in vitro and in vivo is becoming an ever more important means of elucidating pathways of molecular and cellular biochemistry. In addition, gene therapy has been proposed as a novel and potentially powerful treatment for both inherited and acquired diseases. Successful gene transfer and gene blockade generally depend on high efficiency delivery of exogenous DNA or RNA into living cells, and much effort has therefore been focused on the development of methods for achieving this delivery in a safe and effective manner. We describe here our application of fusigenic Sendai virus (HVJ)-liposome technology toward the effective delivery of DNA into vascular smooth muscle cells (VSMC) in cell culture. Cellular uptake and intracellular distribution of oligodeoxynucleotide (ODN) after transfection with HVJ-liposome complexes was characterized using fluorescent (FITC)-labeled ODN, and the biologic effect of HVJ-liposome mediated transfection was demonstrated via inhibition of DNA synthesis in cultured VSMC using antisense ODN against basic fibroblast growth factor.
Mann
M J
MJ
Department of Medicine, Harvard Medical School, USA.
Morishita
R
R
Gibbons
G H
GH
von der Leyen
H E
HE
Dzau
V J
VJ
D000818:Animals; D002642:Chick Embryo; D018014:Gene Transfer Techniques; D008081:Liposomes / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D051381:Rats; D018108:Respirovirus / Q000235:genetics*; D014162:Transfection
D008081:Liposomes; D016376:Oligonucleotides, Antisense