title: Expression of vascular endothelial growth factor in human luteinizing granulosa cells and its correlation with the response to controlled ovarian hyperstimulation.
abstract: Ovulation induction represents one of the most important steps for the success of assisted reproductive technology (ART) procedures. To better understand the mechanisms that regulate follicle growth, oocyte maturation, and ovarian steroidogenesis, we investigated the correlations between vascular endothelial growth factor (VEGF) gene expression in human luteinizing granulosa cells, steroid production and oocyte retrieval in patients undergoing controlled ovarian hyperstimulation. We evaluated the messenger ribonucleic acid (mRNA) for VEGF in human luteinizing granulosa cells obtained at the time of oocyte retrieval from 24 women participating in an in vitro fertilization program at the Reproductive Endocrinology Center of our Department of Obstetrics and Gynecology. We found a positive linear correlation of VEGF mRNA with estradiol and progesterone serum levels at the day of oocyte retrieval (p < 0.05). Furthermore, VEGF mRNA expression was significantly higher in granulosa cells obtained from patients with an elevated number of oocytes and high fertilization rate (p < 0.05). Our data confirm that VEGF may play an important role in the regulation of vascular development during follicular growth and luteal differentiation.
authors.affiliation: Department of Obstetrics and Gynecology, University of Milan, H San Raffaele Scientific Institute, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Doldi
authors.forename: M
authors.initials: M
authors.lastname: Bassan
authors.forename: A
authors.initials: A
authors.lastname: Messa
authors.forename: A
authors.initials: A
authors.lastname: Ferrari
mesh_terms: D003338:Corpus Luteum / Q000502:physiology*; D016228:Endothelial Growth Factors / Q000235:genetics*; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D008222:Lymphokines / Q000235:genetics*; D010062:Ovulation Induction; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D011374:Progesterone; D004958:Estradiol