title: Protein half-lives of calmodulin and the plasma membrane Ca-ATPase in rat brain.
abstract: We report the half-lives for two proteins involved in the regulation of intracellular calcium in the brain: the plasma membrane Ca-ATPase and its regulatory protein, calmodulin. [14C]-labeled leucine was injected into seven month old adult Fischer 344 rats and the time-dependent appearance and loss of radioactivity was monitored in both the serum and proteins from the brains of rats sacrificed from 4 hours to 13 days after injection. Experimental data obtained for calmodulin and the plasma membrane Ca-ATPase are best described by theoretical curves accounting for leucine reutilization that assume apparent half-lives of 18 (+/-2) hours and 12 (+/-1) days, respectively.
authors.affiliation: Department of Biochemistry, University of Kansas, Lawrence 66045, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Ferrington
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: A G
authors.initials: AG
authors.lastname: Krainev
authors.forename: E K
authors.initials: EK
authors.lastname: Michaelis
authors.forename: D J
authors.initials: DJ
authors.lastname: Bigelow
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002147:Calmodulin / Q000096:biosynthesis / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D006207:Half-Life; D007930:Leucine / Q000378:metabolism; D008297:Male; D011865:Radioisotope Dilution Technique; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors
chemical_list: D002147:Calmodulin; D002250:Carbon Radioisotopes; D007930:Leucine