Analysis of the alcABC operon encoding alcaligin biosynthesis enzymes in Bordetella bronchiseptica.
We previously cloned a B. bronchiseptica (Bb) genomic DNA fragment that complements a Bb alcaligin biosynthesis mutant, and reported the identification of a gene, alcA, with predicted protein sequence similarity to siderophore biosynthesis enzymes from other organisms. In the present study we show that further nt sequencing of this region revealed two open reading frames (ORFs) 3' to alcA that encode putative proteins AlcB and AlcC, with significant sequence similarity to the aerobactin biosynthesis enzymes IucB and IucC, respectively. RT-PCR analysis indicated that the three ORFs are encoded on a single transcript, and that this operon is repressed at the transcriptional level by Fe. Primer extension analysis placed the transcriptional start point (tsp) 35 nt from the 5' end of the Fur consensus sequence and 188 nt from the putative start of translation of AlcA.
Giardina
P C
PC
Department of Microbiology and Immunology, State University of New York at Buffalo, 14214, USA.
Foster
L A
LA
Toth
S I
SI
Roe
B A
BA
Dyer
D W
DW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D016950:Bordetella bronchiseptica / Q000201:enzymology / Q000235:genetics*; D016384:Consensus Sequence; D017931:DNA Primers; D005816:Genetic Complementation Test; D006877:Hydroxamic Acids; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016366:Open Reading Frames; D009876:Operon; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D017262:Siderophores / Q000096:biosynthesis*; D014158:Transcription, Genetic
C107670:AlcB protein, Bordetella bronchiseptica; C107671:AlcC protein, Bordetella bronchiseptica; D001426:Bacterial Proteins; D017931:DNA Primers; D006877:Hydroxamic Acids; D017262:Siderophores; C092056:alcaligin