A highly conserved apoptotic suppressor gene is located near the chicken T-cell receptor alpha chain constant region.
A cosmid clone containing the chicken T-cell receptor alpha chain constant region (TCRAC) was sequenced. The cosmid contains the TCRAC gene, six putative joining gene segments (TCRAJ), and surprisingly, a chicken homologue for the human apoptotic suppressor gene, defender against cell death (DAD1). The DAD1 gene is 6.3 kilobases downstream of the TCRAC gene and has an inverted transcription orientation with respect to the TCRAC gene. The cDNA for the chicken DAD1 gene is 597 base pairs in length and encodes a highly conserved hydrophobic protein. The proximal location of DAD1 to the TCRAC locus has also been confirmed in both humans and mouse. The location of the DAD1 gene suggests that DAD1 may play an important role in T-cell related apoptotic activities.
Wang
K
K
Department of Molecular Biotechnology, University of Washington, Box 357730, Seattle, WA 98195, USA.
Gan
L
L
Kuo
C L
CL
Hood
L
L
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D017124:Conserved Sequence; D018899:CpG Islands; D019143:Evolution, Molecular; D005091:Exons; D016153:Genes, Suppressor; D015698:Genomic Library; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012326:RNA Splicing; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D051017:Apoptosis Regulatory Proteins; C489987:Dad1 protein, mouse; D008565:Membrane Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta