title: High-frequency transposition of IS1373, the insertion sequence delimiting the amplifiable element AUD2 of Streptomyces lividans.
abstract: IS1373 is the putative insertion sequence delimiting the amplifiable unit AUD2 of Streptomyces lividans. Two IS1373-derived thiostrepton-resistant transposons, Tn5492 and Tn5494, transposed into multiple sites of the S. lividans chromosome at frequencies as high as 0.4 and 1%, respectively. Hence, IS1373 is a functional insertion sequence and its unique open reading frame, insA, encodes the transposase.
authors.affiliation: Institut für Industrielle Genetik, Universität Stuttgart, Germany. volff@biozentrum.uni-wverzburg.de
authors.forename: J N
authors.initials: JN
authors.lastname: Volff
authors.forename: J
authors.initials: J
authors.lastname: Altenbuchner
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004352:Drug Resistance, Microbial; D005822:Genetic Vectors / Q000235:genetics; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013883:Thiostrepton / Q000494:pharmacology; D014169:Transformation, Bacterial; D019895:Transposases
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; D019895:Transposases; D013883:Thiostrepton