Distinct type-2A protein phosphatases activate HMGCoA reductase and acetyl-CoA carboxylase in liver.
Acetyl-CoA carboxylase and HMGCoA reductase are inactivated by the same AMP-activated protein kinase and are activated by type-2A protein phosphatase. To determine whether the same species of protein phosphatase-2A were involved, we studied the interconversion of acetyl-CoA carboxylase and HMGCoA reductase in isolated rat hepatocytes. We show that (i) these enzymes are differently regulated in hepatocytes and (ii) the species of type-2A protein phosphatase involved in their activation are different and can be separated by anion-exchange chromatography.
Gaussin
V
V
Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Louvain University Medical School, Brussels, Belgium.
Skarlas
P
P
Ching
Y P
YP
Hardie
D G
DG
Hue
L
L
D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D000818:Animals; D015687:Cell Hypoxia; D002845:Chromatography; D004789:Enzyme Activation; D005973:Glutamine / Q000494:pharmacology*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D054648:Protein Phosphatase 2; D017346:Protein Serine-Threonine Kinases; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D009097:Multienzyme Complexes; D005973:Glutamine; D006903:Hydroxymethylglutaryl CoA Reductases; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D000103:Acetyl-CoA Carboxylase