Carbon-13 magnetic resonance spectroscopy of glucose metabolism in SCC-VII tumors.
We used 13C MR spectroscopy to follow the metabolism of [1-13C]glucose in SCC-VII tumors of C3H/HeN mice. Six different SCC-VII tumors and six muscles of normal legs were used. Spectroscopy was carried out with the intraperitoneal injection of 100 mg of [1-13C]glucose. Six distinct resonances were detected: the alpha and beta anomers of the C1 of glucose, C2,3,4 of glutamate and glutamine, and the C3 of lactate. Signals from [1-13C]glucose were first detected in the spectra of tumor 10 minutes after injection. Signals decreased faster in the SCC-VII tumors than the muscle of normal leg. The presence of 13C of glutamate/glutamine suggested the entry of pyruvate into the TCA cycle in SCC-VII tumor. The more rapid decrease of signals from [1-13C]glucose in the SCC-VII tumor may be attributed to the effects of blood flow volume and metabolic activity.
Furusawa
M
M
Department of Radiology, Kumamoto University School of Medicine, Japan.
Onomichi
M
M
Morishita
S
S
Hamatake
S
S
Takahashi
M
M
Ito
Y H
YH
D000818:Animals; D001783:Blood Flow Velocity; D002247:Carbon Isotopes; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008809:Mice, Inbred C3H; D018482:Muscle, Skeletal / Q000378:metabolism; D009368:Neoplasm Transplantation
D002247:Carbon Isotopes; D019344:Lactic Acid; D005947:Glucose