Biotransformation of linoleic acid with the Candida tropicalis M25 mutant.
Linoleic acid was transformed by mutant Candida tropicalis M25 and transformations were studied in batch and fed-batch cultures. Cofermentations with palmitic acid as inducer of the fatty acid degradation pathway were performed. Besides the (Z),(Z)-octadeca-6,9-dienedioic acid, (Z),(Z)-3-hydroxyoctadeca-9,12-dienedioic acid and (Z),(Z)-3-hydroxytetradeca-5,8-dienedioic acid were obtained as the main fermentation products. The maximum concentrations of (Z),(Z)-octadeca-6,9-dienedioic acid and (Z),(Z)-3-hydroxyoctadeca-9,12-dienedioic acid reached values of 6.4 g/l and 6.9 g/l respectively. The structures of the products were characterized by chemical and spectroscopic methods. The configuration of the double bonds was not changed during bioconversion. As only one regioisomer of the hydroxylated fatty acid was detected, the hydroxylation is site-specific.
Fabritius
D
D
Institut für Organische Chemie, Westfälische Wilhelms-Universität, Münster, Germany.
Schäfer
H J
HJ
Steinbüchel
A
A
D001711:Biotransformation; D002175:Candida / Q000378:metabolism*; D003998:Dicarboxylic Acids / Q000378:metabolism*; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D006880:Hydroxy Acids / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010126:Ozone; D019308:Palmitic Acid / Q000378:metabolism
D003998:Dicarboxylic Acids; D006880:Hydroxy Acids; D008041:Linoleic Acids; D019308:Palmitic Acid; D010126:Ozone; D019787:Linoleic Acid