Inhibition of migrating lens epithelial cells by sustained release of ethylenediaminetetraacetic acid.
OBJECTIVE
To evaluate the effect of the sustained-release of ethylenediaminetetraacetic acid (EDTA) chelating Ca++ on lens epithelial cell (LEC) migration.


METHODS
Nishi Eye Hospital, Jinshikai Medical Foundation, Osaka, Japan.


METHODS
Polylactic-glycolic acid disks containing 10% EDTA were placed in saline solution for about 2 weeks in vitro. About 60% (7 micrograms/hour) of the EDTA was released during that time. The disks with a posterior chamber intraocular lens placed above were implanted in the capsular bag in five rabbit eyes after continuous curvilinear capsulorhexis and phacoemulsification. A disk without EDTA and the same lens type were placed in the bag in the contralateral eyes, which served as controls.


RESULTS
After 2 to 3 months, opacification in the central posterior capsule was significantly reduced in all eyes that received the disk with EDTA.


CONCLUSIONS
The deprivation of Ca++ disrupted interaction between the posterior capsule and migrating LECs by inactivating the adhesion molecule integrin synthesized by LECs, significantly reducing LEC migration onto the posterior capsule.
Nishi
O
O
Nishi Eye Hospital, Osaka, Japan.
Nishi
K
K
Saitoh
I
I
Sakanishi
K
K
D000818:Animals; D001672:Biocompatible Materials; D002386:Cataract / Q000473:pathology / Q000517:prevention & control; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002614:Chelating Agents / Q000494:pharmacology*; D003692:Delayed-Action Preparations; D004492:Edetic Acid / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005500:Follow-Up Studies; D019344:Lactic Acid; D007903:Lens Capsule, Crystalline / Q000187:drug effects* / Q000473:pathology; D007910:Lenses, Intraocular; D018918:Phacoemulsification; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011817:Rabbits
D001672:Biocompatible Materials; D002614:Chelating Agents; D003692:Delayed-Action Preparations; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004492:Edetic Acid