Expression of serotonin transporter mRNA in rat brain: presence in neuronal and non-neuronal cells and effect of paroxetine.
The expression of serotonin transporter mRNA in rat brain was-examined by in situ hybridisation. Hybridisation was observed in cells of the known serotonergic nuclei and no other neuronal populations. It was also associated with ependymal cells of the aqueduct which may indicate a specialisation of this part of the ventricular system in anatomical and neurophysiological terms. The effect of the selective serotonin reuptake inhibitor paroxetine on neuronal expression of the serotonin transporter mRNA was examined. Quantitation at the cellular level in the dorsal and median raphe nuclei was carried out by analysis of the mean number of silver grains per cell in autoradiographed sections. No significant change (P > 0.1) in serotonin transporter mRNA expression was observed following 21 day administration of paroxetine (5 mg/kg per day).
Swan
M C
MC
Department of Anatomy and Cell Biology, Imperial College School of Medicine at St Mary's, London, UK.
Najlerahim
A R
AR
Bennett
J P
JP
D000818:Animals; D001345:Autoradiography; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D017374:Paroxetine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D011903:Raphe Nuclei / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology*
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D017374:Paroxetine; D012333:RNA, Messenger; D012701:Serotonin; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors; C493232:Slc6a4 protein, rat