title: Salmonella enterica var Typhimurium and Salmonella enterica var Enteritidis express type 1 fimbriae in the rat in vivo.
abstract: In a series of experiments rats were dosed with purified type 1 fimbriae from Salmonella enterica var Enteritidis or with fimbriated cultures of either S. enterica var Typhimurium or S. enterica var Enteritidis. Paraffin-wax embedded histological sections of jejunal and ileal tissue were taken and stained by the streptavidin biotin complex (sABC) staining technique for the detection of salmonella and type 1 fimbriae. On oral infection with Enteritidis and Typhimurium both bacteria were shown to be closely associated with the rat ileal epithelium and expressed type 1 fimbriae, thus clearly demonstrating that type 1 fimbriae are expressed by salmonellae in vivo. Moreover, association with the ileum was also shown to occur when purified type 1 fimbriae were orally administered to rats. Our results suggest that type 1 fimbriae alone or in combination with other fimbriae may play an important role in the early stages of infection with these pathogenic bacteria.
authors.affiliation: Department of Pathology, Medical School, University of Aberdeen, UK.
authors.forename: S W
authors.initials: SW
authors.lastname: Ewen
authors.forename: P J
authors.initials: PJ
authors.lastname: Naughton
authors.forename: G
authors.initials: G
authors.lastname: Grant
authors.forename: M
authors.initials: M
authors.lastname: Sojka
authors.forename: E
authors.initials: E
authors.lastname: Allen-Vercoe
authors.forename: S
authors.initials: S
authors.lastname: Bardocz
authors.forename: C J
authors.initials: CJ
authors.lastname: Thorns
authors.forename: A
authors.initials: A
authors.lastname: Pusztai
mesh_terms: D000818:Animals; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D051381:Rats; D012477:Salmonella enteritidis / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000378:metabolism*