[A new class of small RNP (alpha-RNP) containing antisense RNA in K-562 cells. III. The DNA-binding activity of nuclear alpha-RNP. The Signal Transmission from Growth Factors Group of the Medical Faculty, F. Schiller University, Jena, Germany].
DNA-binding activity of small nuclear alpha-RNP identified in acid-soluble fraction of chromatin of human proerythroleukemic cell line K-562 was studied using the technique of gel retardation. We found that nuclear alpha-RNP isolated from K-562 cells through treatment with dimethylsulfoxide, an agent inducing differentiation, acquire a capacity to specific interaction with Alu repeats of DNA leading to the formation of alpha-RNP-Alu-DNA complexes; nuclear alpha-RNP from cells that were not treated with dimethylsulfoxide do not show such capacity, although they are tightly bound with chromatin in the cell. Thus, the capacity of nuclear alpha-RNP to direct interaction with DNA Alu repeats appearing after the induction of K-562 cells to differentiation along erythroid pathway is an inducible property. We discuss hypothesis about the involvement of nuclear alpha-RNP in the control of expression of inducible genes at the level of chromatin and interaction with DNA.
Konstantinova
I M
IM
Wetzker
R
R
Petukhova
O A
OA
Chesnokov
I N
IN
Volkova
I V
IV
Turoverova
L V
LV
Ermolaeva
Iu B
IuB
Gauze
L N
LN
D001483:Base Sequence; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016372:RNA, Antisense / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017411:Ribonucleoproteins, Small Nuclear / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D002843:Chromatin; D015342:DNA Probes; D004273:DNA, Neoplasm; D016372:RNA, Antisense; D017411:Ribonucleoproteins, Small Nuclear; D004121:Dimethyl Sulfoxide