title: Peptide bank generated by large-scale preparation of circulating human peptides.
abstract: Human hemofiltrate (HF) is a source for the purification of circulating regulatory peptides. HF is obtained in large quantities during treatment of patients suffering from chronic renal failure. We have developed a large-scale method for separating peptides from amounts up to 10,000 1 HF into 300 fractions in a standardized two-step procedure, employing cation-exchange separation, followed by reversed-phase chromatography. These fractions represent a peptide bank containing bioactive, desalted and lyophilized peptides of blood. Screening for and isolation of regulatory human peptides is simplified by using this peptide bank.
authors.affiliation: Niedersächsisches Institut für Peptid-Forschung GmbH, Hannover, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Schulz-Knappe
authors.forename: M
authors.initials: M
authors.lastname: Schrader
authors.forename: L
authors.initials: L
authors.lastname: Ständker
authors.forename: R
authors.initials: R
authors.lastname: Richter
authors.forename: R
authors.initials: R
authors.lastname: Hess
authors.forename: M
authors.initials: M
authors.lastname: Jürgens
authors.forename: W G
authors.initials: WG
authors.lastname: Forssmann
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D016208:Databases, Factual; D006440:Hemofiltration; D006801:Humans; D007676:Kidney Failure, Chronic / Q000652:urine; D010455:Peptides / Q000097:blood* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010455:Peptides