A recombinant protein from Schistosoma mansoni useful for the detection of S. mansoni and Schistosoma haematobium antibodies.
A recombinant Schistosoma mansoni protein has been identified as a useful antigen for the detection of S. mansoni and Schistosoma haematobium antibodies. The purified recombinant protein, Sm22.3, was assayed using an enzyme-linked immunosorbent assay format against a battery of 491 well defined sera, including S. mansoni, S. haematobium, and Schistosoma japonicum infection sera, normal human sera, sera from 9 other parasitic infections, and sera from 2 additional infections. The sensitivity for detecting S. mansoni and S. haematobium infections with this single recombinant protein is 80.1%. The specificity is 94.8%. However, 15 of the 16 cross-reactive sera are malaria infection sera, and we have data suggesting that these malaria sera are actually recognizing an epitope on the vector-derived 6Xhistidine tag of recombinant Sm22.3. If this is the case, then, the actual specificity of the assay is 99.6%.
Hancock
K
K
Centers for Disease Control and Prevention, Division of Parasitic Diseases, Atlanta, Georgia 30341-3724, USA.
Mohamed
Y B
YB
Haichou
X
X
Noh
J
J
Dotson
E M
EM
Tsang
V C
VC
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008253:Macaca mulatta; D008288:Malaria / Q000276:immunology; D008969:Molecular Sequence Data; D010961:Plasmodium / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012548:Schistosoma haematobium / Q000235:genetics / Q000276:immunology*; D012549:Schistosoma japonicum / Q000276:immunology; D012550:Schistosoma mansoni / Q000235:genetics / Q000276:immunology*; D012553:Schistosomiasis haematobia / Q000175:diagnosis / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000175:diagnosis / Q000276:immunology; D012680:Sensitivity and Specificity; D013045:Species Specificity
D000909:Antibodies, Helminth; D000913:Antibodies, Protozoan; D000947:Antigens, Helminth; D018509:DNA, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins