In vitro and in vivo properties of murine monoclonal antibody for a novel immature thymocyte-differentiated antigen, JL1.
JL1 is a novel thymocyte-differentiated antigen strictly confined to stage II immature cortical thymocytes. It is expressed in several types of leukemias and lymphomas. Murine anti-JL1 monoclonal antibody labeled with 131I and 99mTc showed 60-70% of immunoreactivity and 1.4-1.9 x 10(9) L/mol of affinity constant. The incubation of the radiolabeled antibody with Molt-4 cells showed no evidence of modulation or shedding. Localization indices increased from day 3 to day 5 in SCID mice bearing Molt-4 cells.
Chung
J K
JK
Department of Nuclear Medicine, Seoul National University Hospital, Korea. jkchung@power2.snu.ac.kr
So
Y
Y
Hong
M K
MK
Choi
S R
SR
Jeong
J M
JM
Lee
D S
DS
Lee
M C
MC
Koh
C S
CS
Choi
E Y
EY
Park
S H
SH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000493:pharmacokinetics; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis* / Q000276:immunology; D019337:Hematologic Neoplasms / Q000000981:diagnostic imaging*; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D016719:Radioimmunodetection; D013667:Technetium
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D007457:Iodine Radioisotopes; C114952:JL1 antigen; D013667:Technetium