title: The large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) is associated with the virion tegument and has PK activity.
abstract: The large subunit of herpes simplex virus type 2 (HSV-2) ribonucleotide reductase (ICP10) was identified in sucrose gradient-purified HSV-2 virions by immunoprecipitation/immunoblotting with antibody specific for the protein kinase (PK) domain. Immunoblotting of individual gradient fractions indicated that ICP10 cosediments with the major capsid protein and the highest virus titers. ICP10 was not labeled by iodination of purified virions, indicating that it is not located on the virion surface. After envelope glycoproteins were removed by detergent treatment, ICP10 was associated with capsid-tegument particles and became sensitive to trypsin digestion. The capsid-tegument-associated ICP10 was phosphorylated and had PK activity in vitro and on Immobilon membranes. A mutant ICP10 protein deleted in the PK domain (p95) was also associated with purified virions (ICP10deltaPK virus) but it lacked PK activity. The data indicate that ICP10 is contained within the tegument component where it retains intrinsic PK activity.
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Smith
authors.forename: L
authors.initials: L
authors.lastname: Aurelian
mesh_terms: D018258:Herpesvirus 2, Human / Q000502:physiology*; D006801:Humans; D011494:Protein Kinases / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D012264:Ribonucleotide Reductases / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014771:Virion / Q000502:physiology*; D014779:Virus Replication
chemical_list: C100671:ICP10 protein, herpes simplex virus type 2; D012264:Ribonucleotide Reductases; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases