[Designing the latex test system for express identification of legionella in the external medium and clinical materials].
A highly sensitive latex test system for identification of Legionella in the external medium and clinical materials have been designed. Protein antigens and polysaccharide components of the outer membrane of the agent were analyzed. Proteins having a molecular mass of 45, 29, and 24 kDa, as well as a polysaccharide component of LPS were found to be common for all L. pneumophila species. Highly affinic immunoglobulins to the antigenic components obtained were covalently linked with latex particles. The test system developed does not give cross-reactions with other microorganisms. The sensitivity of the system is 10(4) COE/ml. Testing water and clinical material samples confirmed that the developed system is more sensitive than the bacteriological method and the direct fluorescence test. In addition, the system is simple to use, cost-effective, it requires little time (no more than 5 min).
Eruslanov
B V
BV
State Scientific Center of Applied Microbiology, Obolensk.
Borzenkov
V N
VN
Pecherskikh
E I
EI
Svetoch
E A
EA
Urakov
N N
NN
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D016952:Legionella pneumophila / Q000276:immunology*; D007876:Legionellosis / Q000175:diagnosis* / Q000276:immunology; D008856:Microscopy, Fluorescence; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins