Effect of human vitreous and hyalocyte-derived factors on vascular endothelial cell growth.
OBJECTIVE
In the present study we have examined the effects of human vitreous and hyalocyte-conditioned medium on human umbilical vein endothelial cells (HUVEC).


METHODS
Human umbilical vein endothelial cells were cultured and treated in quadruplicate with human vitreous and hyalocyte-conditioned medium at different concentrations. Cell numbers were counted on days 1, 3, 5 and 7. Morphological changes and the viability of cells after treatment were also monitored.


RESULTS
The results indicate that both human vitreous and hyalocyte-conditioned medium inhibit proliferation and reduce the viability of HUVEC in vitro. These inhibitory effects were dose- and time-dependent.


CONCLUSIONS
The observations suggest that human hyalocytes and vitreous contain anti-angiogenic factors that influence vascular endothelial cell-growth. These results, combined with those of previous studies, may yield important information about the functional role of vitreous and hyalocytes in intraocular vascular regression.
Zhu
M
M
Department of Clinical Ophthalmology, University of Sydney, New South Wales, Australia. meidong@pc-0.eye.usyd.edu.au
Penfold
P L
PL
Madigan
M C
MC
Billson
F A
FA
D000328:Adult; D002452:Cell Count; D018929:Cell Culture Techniques; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D016228:Endothelial Growth Factors / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005500:Follow-Up Studies; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology; D014471:Umbilical Veins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014822:Vitreous Body / Q000196:embryology / Q000502:physiology*
D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors