Competitive RT-PCR for studying gene expression in micro biopsies.
The technique of RT-PCR has become a powerful method for studying gene expression in various species and tissues. Here we present a competitive PCR for quantitative measurement of absolute levels of mRNA molecules for the regulatory alpha 2/delta subunit of the high-voltage-activated dihydropyridine sensitive human cardiac L-type calcium channel. With the method described the dynamic process of cardiac gene expression can be studied in human endomyocardial biopsies in different patient collectives and even in the individual.
Hullin
R
R
Klinikum Grosshadern, Medizinische Klinik I, Ludwig Maximilians Universitat, Munchen, Germany.
Asmus
F
F
Steinbeck
G
G
D030161:Avian Proteins; D001706:Biopsy / Q000379:methods; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics; D009202:Cardiomyopathies / Q000235:genetics / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics; D009206:Myocardium / Q000737:chemistry / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods*
D030161:Avian Proteins; C051235:CCC protein, chicken; D015220:Calcium Channels; D008565:Membrane Proteins; D009124:Muscle Proteins