title: Interleukin-8 release from human gestational tissue explants: the effects of lipopolysaccharide and cytokines.
abstract: Interleukin (IL)-8 is a chemotactic cytokine that has been implicated in the etiology of infection-induced and normal human labor. In particular, IL-8 has been implicated in the processes of cervical ripening and rupture of fetal membranes because of its role in neutrophil activation and release of cellular matrix remodeling enzymes. In this study, we tested the hypotheses that IL-8 is released locally in the intrauterine environment from human amnion, choriodecidua, and placenta, and that IL-8 release from these tissues is increased by bacterial endotoxin, lipopolysaccharides (LPS), and inflammatory cytokines. IL-8 was released from human amnion, choriodecidual, and placental explants, with choriodecidua demonstrating the most abundant release. IL-8 release was significantly (multiple analysis of variance, p < 0.05) increased by LPS in a time- and dose-dependent manner from both choriodecidual and placental explants, but not from amnion explants. In addition, IL-1alpha (0.28 nM) and tumor necrosis factor alpha (TNFalpha, 10 nM) significantly (Student's t-test, p < 0.05) increased IL-8 release from placental explants 2- to 3-fold. These studies establish that the amnion, choriodecidua, and placenta are a source of IL-8 and demonstrate tissue-specific and differential regulation of IL-8 release by LPS, IL-1alpha, and TNF-alpha. These data support a role for IL-8 in a cascade of inflammatory events initiated by an intrauterine infection and resulting in activation of the labor process.
authors.affiliation: Perinatal Research Centre, Department of Perinatal Medicine, The Royal Women's Hospital, Carlton, Victoria, Australia. n.laham@microbiology.unimelb.edu.au
authors.forename: N
authors.initials: N
authors.lastname: Laham
authors.forename: S P
authors.initials: SP
authors.lastname: Brennecke
authors.forename: G E
authors.initials: GE
authors.lastname: Rice
mesh_terms: D000650:Amnion / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D002823:Chorion / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D046508:Culture Techniques; D016207:Cytokines / Q000494:pharmacology; D003656:Decidua / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D016209:Interleukin-8 / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D010920:Placenta / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D007375:Interleukin-1; D016209:Interleukin-8; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D007770:L-Lactate Dehydrogenase