Superoxide dismutase activity in lymphocytes and polymorphonuclear cells of diabetic patients.
Copper-zinc superoxide dismutase (Cu,Zn-superoxide dismutase) activity was evaluated in lymphocytes and polymorphonuclear cells of insulin-dependent (n = 33) and non-insulin-dependent (n = 34) diabetic patients. A commercial method for the measurement of superoxide dismutase activity was adapted for use on a discrete analyser and evaluated for interference by other antioxidants with superoxide anion-scavenging properties. In comparison to healthy control subjects (n = 32), a significantly lower Cu,Zn-superoxide dismutase activity was found in both lymphocytes and polymorphonuclear cells of insulin-dependent (2.08 +/- 0.58 vs. 1.70 +/- 0.46 U/mg protein, p < 0.05, and 1.06 +/- 0.46 vs. 0.64 +/- 0.40 U/mg protein, p < 0.001, respectively) and non-insulin-dependent diabetic patients (2.08 +/- 0.58 vs. 1.61 +/- 0.48 U/mg protein, p < 0.01, and 1.06 +/- 0.46 vs. 0.53 +/- 0.24 U/mg protein, p < 0.001, respectively). There was a week, but significant negative correlation between age and Cu,Zn-superoxide dismutase activity in lymphocytes and polymorphonuclear cells (r = -0.22 and r = -0.28, p < 0.05, respectively), whereas no influence of gender, diabetes duration and glycaemic control was observed. The results indicate that diabetes mellitus could elicit a significant disturbance in superoxide anion-scavenging potential of lymphocytes and polymorphonuclear cells.
Vucić
M
M
Department of Laboratory Medicine, Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, Medical Faculty, University of Zagreb, Croatia.
Gavella
M
M
Bozikov
V
V
Ashcroft
S J
SJ
Rocić
B
B
D000328:Adult; D000375:Aging / Q000097:blood; D000975:Antioxidants / Q000378:metabolism; D001774:Blood Chemical Analysis / Q000379:methods; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000201:enzymology* / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000097:blood; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology*; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood*
D000975:Antioxidants; D016166:Free Radical Scavengers; D013482:Superoxide Dismutase