Cloning and mutational analysis of the gene for the stationary-phase inducible catalase (catC) from Pseudomonas putida.
Pseudomonas putida, a bacterium that colonizes plant roots and enhances plant growth, produces three isozymes of catalase (A, B, and C) in stationary-phase cells. A catalase probe, generated by PCR analysis of P. putida genomic DNA with oligomers based on typical catalase sequences, hybridized to a genomic clone that expressed catalase C in Escherichia coli. The catC gene from this clone had a 2,133-bp open reading frame with a high level of identity to the stationary-phase-specific E. coli katE. Chromosomal mutants of P. putida deficient in catalase C, obtained by gene interruption with a luxAB-npt cassette, demonstrated enhanced catC transcription in stationary-phase cells and, upon exposure to phenol, in logarithmic-phase cells. The catalase C-deficient cells were not impaired in their ability to colonize roots of bean or wheat plants grown under sterile conditions.
Miller
C D
CD
Department of Biology, Utah State University, Logan 84322-5305, USA.
Kim
Y C
YC
Anderson
A J
AJ
D000595:Amino Acid Sequence; D002374:Catalase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D018517:Plant Roots / Q000382:microbiology; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014158:Transcription, Genetic
D007527:Isoenzymes; D011994:Recombinant Proteins; D002374:Catalase