Leukocyte mobilization to skin lesions, determination of cell surface receptors (CD11b/CD18) and phagocytic capacities of neutrophils in dogs with chronic deep pyoderma.
A suction blister technique was used in eight dogs with chronic deep pyoderma to determine chemotaxis in vivo. By flow cytometry the expression of adhesion molecules (CD11b/CD18) on exudative and peripheral neutrophils were analyzed in 11 healthy dogs and six dogs with chronic deep pyoderma. Phagocytosis in vitro capacities of exudative and peripheral neutrophils were analyzed in six healthy dogs and six dogs with chronic deep pyoderma. Dogs with chronic pyoderma showed significantly better chemotaxis in vivo compared with the healthy dogs (P < 0.05). Expression of adhesion molecules CD11b and CD18, and phagocytosis was significantly (P < 0.05) better in the dogs with pyoderma compared with the healthy dogs. In both groups exudative cells expressed significantly (P < 0.05) more CD11b/CD18 receptors compared with blood neutrophils. We conclude that there are no serious functional disturbances detectable in the peripheral neutrophils, nor in the exudative neutrophils from dogs with chronic deep pyoderma.
Wisselink
M A
MA
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
van Kessel
K P
KP
Willemse
T
T
D000818:Animals; D018821:CD18 Antigens / Q000032:analysis*; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D002908:Chronic Disease; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D005434:Flow Cytometry / Q000662:veterinary; D016177:Macrophage-1 Antigen / Q000032:analysis*; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D010587:Phagocytosis / Q000276:immunology*; D011711:Pyoderma / Q000276:immunology* / Q000473:pathology / Q000662:veterinary*
D018821:CD18 Antigens; D016177:Macrophage-1 Antigen