Dietary diosgenin attenuates subacute intestinal inflammation associated with indomethacin in rats.
We investigated the effects of dietary diosgenin (Dio), a plant-derived sapogenin, on indomethacin (Indo)-induced intestinal inflammation and alterations in bile secretion in rats. In anesthetized rats, bile secretion, intestinal inflammation, and blood chemistry were assessed 3 days after two subcutaneous injections of Indo given 24 h apart. Dio (> 80 mg.kg-1.day-1) pretreatment significantly inhibited weight and food intake decreases and intestinal inflammation. This protective effect was confirmed by examination of gross and histological findings and intestinal myeloperoxidase activity. Dio significantly increased biliary cholesterol (Chol) output and prevented the decreases in bile flow, bile acid output, and biliary alpha-muricholic acid and the increases in biliary hyodeoxycholic acid, deoxycholic acid, and hydrophobicity index of bile. Significantly more biliary Chol and phospholipids were present in macromolecules separate from bile acids and Indo in Dio-treated rats. Dio significantly increased the elimination constant of Indo and reduced plasma Indo levels at 3 and 12 h but did not influence biliary secretion of Indo for 3.5 h after injection. Although Dio dose-dependently attenuated subacute intestinal inflammation and normalized bile secretion in this model, it may also compromise the anti-inflammatory action of indo.
Yamada
T
T
First Department of Internal Medicine, Nagoya City University Medical School, Aichi, Japan.
Hoshino
M
M
Hayakawa
T
T
Ohhara
H
H
Yamada
H
H
Nakazawa
T
T
Inagaki
T
T
Iida
M
M
Ogasawara
T
T
Uchida
A
A
Hasegawa
C
C
Murasaki
G
G
Miyaji
M
M
Hirata
A
A
Takeuchi
T
T
D000208:Acute Disease; D000818:Animals; D001646:Bile / Q000187:drug effects / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000737:chemistry; D001769:Blood / Q000187:drug effects / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000494:pharmacology; D004032:Diet; D004144:Diosgenin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D004751:Enteritis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D005374:Filtration; D007213:Indomethacin / Q000097:blood; D051381:Rats
D001647:Bile Acids and Salts; D002784:Cholesterol; D004144:Diosgenin; D007213:Indomethacin