Polymorphonuclear leukocyte-mediated bone degradation and influence of blood mononuclear cells on the mouse calvarial model.
OBJECTIVE
To investigate the relationships between degradation of bone and activated blood polymorphonuclear leukocytes (PMNL) and mononuclear leukocytes (ML) as well as their soluble products in vitro.


METHODS
A neonatal mouse calvarial bone model was used to assess the activity of degradation (by measuring the amount of 45Ca release) by normal human blood leukocytes, separated PMNL and ML following 24-hour incubation. The effects of conditioned culture medium obtained from Staphylococcus aureus-stimulated ML on PMNL-mediated calvarial bone loss were also studied.


RESULTS
It was demonstrated that isolated human PMNL rapidly degraded bone in a dose and time dependent manner. The PMNL-mediated bone degradation was enhanced by conditioned medium obtained from Staphylococcus aureus-stimulated ML.


CONCLUSIONS
These findings implicate PMNL as major contributors to early bone loss in infectious diseases such as acute haematogenous osteomyelitis.
Wang
Y H
YH
Department of Surgery, Wellington School of Medicine, New Zealand.
Allardyce
R A
RA
Rogers
G R
GR
Gillespie
W J
WJ
Doyle
J S
JS
D000818:Animals; D001862:Bone Resorption / Q000209:etiology*; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D046508:Culture Techniques; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology; D051379:Mice; D009504:Neutrophils / Q000502:physiology*; D010019:Osteomyelitis / Q000473:pathology*; D012886:Skull / Q000473:pathology; D013203:Staphylococcal Infections / Q000473:pathology*
D017077:Culture Media, Conditioned