title: Changes with age in the influence of endogenous nitric oxide on transport properties of the rabbit aortic wall near branches.
abstract: Uptake of circulating albumin by the aortic wall is greater downstream than upstream of branches in immature rabbits, but the opposite pattern occurs in mature animals. We investigated the role of NO in determining these variations. Descending thoracic aortas of rabbits were cannulated using techniques that avoid depressurization, overstretching, and excessive fluid dynamic stresses at the endothelial surface. They were perfused in situ at a constant pressure and flow rate with oxygenated, protein-containing physiological buffer, with or without N omega-monomethyl-L-arginine, an inhibitor of NO synthesis. Aortas were fixed 7 to 8 minutes after the addition of rhodamine-labeled albumin to this perfusate, and uptake of the tracer near intercostal ostia was measured by digital imaging fluorescence microscopy of sections through the wall. Despite the absence of pulsatile flow, blood cells, and many plasma components, patterns of transport in control experiments were the same as those occurring in vivo; uptake was greatest downstream of ostia in immature vessels and upstream in mature ones, although mean uptake was higher than previously reported. In the presence of the inhibitor, mean uptake in immature arteries was elevated threefold and the maximum tracer concentration occurred deeper in the wall, but there was no change in the fractional difference between regions. Conversely, the reverse of the control pattern of transport was observed in mature arteries exposed to the inhibitor, but there was no change in mean uptake. The reversal was almost entirely prevented by adding excess L-arginine to the perfusate and was largely stereospecific. Endogenous NO thus appears to determine the mature pattern of transport near branches and helps to maintain the barrier function of the immature wall.
authors.affiliation: School of Animal and Microbial Sciences, University of Reading, United Kingdom.
authors.forename: B A
authors.initials: BA
authors.lastname: Forster
authors.forename: P D
authors.initials: PD
authors.lastname: Weinberg
mesh_terms: D000367:Age Factors; D000818:Animals; D001011:Aorta / Q000033:anatomy & histology / Q000378:metabolism*; D001692:Biological Transport; D001783:Blood Flow Velocity; D001794:Blood Pressure; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010477:Perfusion; D011817:Rabbits; D012212:Rheology; D012709:Serum Albumin / Q000378:metabolism; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D012709:Serum Albumin; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase