Isolation from fetal bovine serum of a fragment b of complement factor B-like protein improving a long-term survival of human endothelial cells.
It is known that serum is a most important factor supporting cell survival and growth. Particularly, the deprivation of serum would result in the death of human endothelial cell. Our previous paper reported an endothelial cell-viability maintaining factor (EC-VMFa) purified from fetal bovine serum and identified as an apolipoprotein. In the present further study, it is demonstrated that another potent serum factor (refer as EC-VMFb) is also possessed of the endothelial cell-viability maintaining activity, improving a long-term survival of human endothelial cells in serum-free medium. EC-VMFb has a molecular weight of 66,000 (reduced and nonreduced), pI of 4.5 and has been identified as fragment b of complement factor B (Bb)-like protein by amino-terminal amino acid sequence.
Cai
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Research Institute for the Functional Peptides, 11-26 Minamisanbancho, Yamagata, 990, Japan. caigp@mail.tsinghua.edu.cn
Satoh
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Hoshi
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D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D011415:Complement Factor B / Q000737:chemistry*; D016895:Culture Media, Serum-Free; D004730:Endothelium, Vascular / Q000166:cytology*; D005312:Fetal Blood / Q000737:chemistry*; D006023:Glycoproteins; D006801:Humans; D007526:Isoelectric Point; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017385:Sequence Homology; D014471:Umbilical Veins; D053482:beta 2-Glycoprotein I
D016895:Culture Media, Serum-Free; D006023:Glycoproteins; D010446:Peptide Fragments; D053482:beta 2-Glycoprotein I; D011415:Complement Factor B