A molecular scheme based on 23S rRNA gene polymorphisms for rapid identification of Campylobacter and Arcobacter species.
23S rRNA gene PCR amplicons from 118 strains representing 15 species of Campylobacter and four species of Arcobacter were consecutively digested with HpaII, CfoI, and HinfI. A reproducible and discriminatory identification scheme based on combined restriction patterns was developed by using reference strains and was applied to the identification of a variety of isolates.
Hurtado
A
A
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, United Kingdom.
Owen
R J
RJ
D002167:Campylobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S