title: Tromp1, a putative rare outer membrane protein, is anchored by an uncleaved signal sequence to the Treponema pallidum cytoplasmic membrane.
abstract: Treponema pallidum rare outer membrane protein 1 (Tromp1) has extensive sequence homology with substrate-binding proteins of ATP-binding cassette transporters. Because such proteins typically are periplasmic or cytoplasmic membrane associated, experiments were conducted to clarify Tromp1's physicochemical properties and cellular location in T. pallidum. Comparison of the sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobilities of (i) native Tromp1 and Tromp1 synthesized by coupled in vitro transcription-translation and (ii) native Tromp1 and recombinant Tromp1 lacking the N-terminal signal sequence revealed that the native protein is not processed. Other studies demonstrated that recombinant Tromp1 lacks three basic porin-like properties: (i) the ability to form aqueous channels in liposomes which permit the influx of small hydrophilic solutes, (ii) an extensive beta-sheet secondary structure, and (iii) amphiphilicity. Subsurface localization of native Tromp1 was demonstrated by immunofluorescence analysis of treponemes encapsulated in gel microdroplets, while opsonization assays failed to detect surface-exposed Tromp1. Incubation of motile treponemes with 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)-diazarine, a photoactivatable, lipophilic probe, also did not result in the detection of Tromp1 within the outer membranes of intact treponemes but, instead, resulted in the labeling of a basic 30.5-kDa presumptive outer membrane protein. Finally, analysis of fractionated treponemes revealed that native Tromp1 is associated predominantly with cell cylinders. These findings comprise a body of evidence that Tromp1 actually is anchored by an uncleaved signal sequence to the periplasmic face of the T. pallidum cytoplasmic membrane, where it likely subserves a transport-related function.
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235, USA.
authors.forename: D R
authors.initials: DR
authors.lastname: Akins
authors.forename: E
authors.initials: E
authors.lastname: Robinson
authors.forename: D
authors.initials: D
authors.lastname: Shevchenko
authors.forename: C
authors.initials: C
authors.lastname: Elkins
authors.forename: D L
authors.initials: DL
authors.lastname: Cox
authors.forename: J D
authors.initials: JD
authors.lastname: Radolf
mesh_terms: D001389:Azirines; D001426:Bacterial Proteins; D002462:Cell Membrane / Q000378:metabolism*; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D008081:Liposomes / Q000378:metabolism; D009895:Opsonin Proteins; D018272:Porins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000378:metabolism*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014210:Treponema pallidum / Q000378:metabolism*
chemical_list: D001389:Azirines; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D008081:Liposomes; D009895:Opsonin Proteins; D018272:Porins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C093836:Tromp1 protein, Treponema pallidum; C033174:3-(trifluoromethyl)-3-(3-iodophenyl)diazirine