Mice with a targeted disruption of the neurotrophin receptor trkB lose their gustatory ganglion cells early but do develop taste buds.
The alleged ability of taste afferents to induce taste buds in developing animals is investigated using a mouse model with a targeted deletion of the tyrosine kinase receptor trkB for the neurotrophin BDNF. This neurotrophin was recently shown to be expressed in developing taste buds and the receptor trkB has been shown to be expressed in the developing ganglion cells that innervate the taste buds. Our data show a reduction of geniculate ganglion cells to about 5% of control animals in neonates. Degeneration of ganglion cells starts when processes reach the central target (solitary tract) but before they reach the peripheral target (taste buds). Degeneration of ganglion cells is almost completed in trkB knockout mice before taste afferents reach in control animals the developing fungiform papillae. Four days later the first taste buds can be identified in fungiform papillae of both control and trkB knockout mice in about equal number and density. Many taste buds undergo a normal maturation compared to control animals. However, the more lateral and caudal fungiform papillae grow less in size and become less conspicuous in older trkB knockout mice. No intragemmal innervation can be found in trkB knockout taste buds but a few extragemmal fibers enter the apex and end between taste had cells without forming specialized synapses. Taste buds of trkB knockout mice appear less well organized than those of control mice, but some cells show similar vesicle accumulations as control taste bud cells in their base but no synaptic contact to an afferent. These data strongly suggest that the initial-development of many fungiform papillae and taste buds is independent of the specific taste innervation. It remains to be shown why others appear to be more dependent on proper innervation.
Fritzsch
B
B
Creighton University, School of Medicine, Dept. of Biomedical Sciences, Omaha, NE 68178, USA.
Sarai
P A
PA
Barbacid
M
M
Silos-Santiago
I
I
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002452:Cell Count; D002814:Chorda Tympani Nerve / Q000166:cytology / Q000502:physiology; D005154:Facial Nerve / Q000166:cytology / Q000502:physiology; D017950:Ganglia, Sensory / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D009412:Nerve Fibers / Q000502:physiology; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D013650:Taste Buds / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D014059:Tongue / Q000254:growth & development / Q000294:innervation / Q000648:ultrastructure
D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases