Structure and dynamics of the glucocorticoid receptor DNA-binding domain: comparison of wild type and a mutant with altered specificity.
Nuclear magnetic resonance was used to compare parameters reflecting solution structure and dynamics of the glucocorticoid receptor DNA-binding domain (GRDBD), which binds specifically to a GRE binding site on DNA, and a triple mutant (GRDBDEGA), which binds to an ERE site. The studies were prompted by an earlier observation that the cooperativity for dimeric DNA-binding is 10 times higher for the GRDBDEGA-ERE association than for the GRDBD-GRE association (Lundbäck et al., 1994). The higher binding cooperativity of the mutant was unexpected since the triple mutation (G458E, S459G, and V462A) is made in the recognition helix and distant from the dimerization surface which is formed by residues in the fragment A477-N491. Sequential and long-range NOE connectivities and measured 3JHNHalpha coupling constants indicate that the overall structures of the two proteins are very similar, possibly with a less well-defined structure of the fragment K486-N491 in GRDBDEGA. However, chemical shift changes, line broadening, and increased amide proton exchange rates are observed for several residues at, or close to, the dimerization surface of the mutant. These observations are interpreted as a lower stability and/or several slowly interconverting folded conformations of this region of GRDBDEGA. The effects are likely to be due to the loss of a hydrogen bond which links S459 to the dimerization region in GRDBD. Different mechanisms for the increased binding cooperativity of the mutant are discussed, and it is noted that the properties of the GRDBDEGA dimerization region are reminiscent of those reported for the estrogen receptor DBD, which also binds to an ERE site.
Berglund
H
H
Center for Structural Biochemistry, Royal Institute of Technology and Karolinska Institute, Novum, S-141 57 Huddinge, Sweden.
Wolf-Watz
M
M
Lundbäck
T
T
van den Berg
S
S
Härd
T
T
D000595:Amino Acid Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D017433:Protein Structure, Secondary; D011965:Receptors, Glucocorticoid / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D004247:DNA