[Immunoglobulin super-gene family and its related genes in detecting MRD in ALL].
OBJECTIVE
To study the rearrangements of the immunoglobulin super-gene family and its related genes, as well as their application in the detection of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL).


METHODS
15 ALL cases including 11 B-ALLs and 3 T-ALLs were studied separately by using immunophenotype detection and molecular clonging methods.


RESULTS
Using DNA Southern blot analysis, we found that the rearrangement of IgH gene existed in 11 B-lineage ALL cases. The specific PCR band was visualized through amplification of the CDR3 region and J region of IgH gene. Furthermore, we sequenced the V-D-J junctional region of IgH gene in 3 B-lineage patients and synthesized two probes against the V-D-J junctional region in order to detect MRD. The hybridization results showing the sensitive of the two probes were 10(-4). In addition, we performed comparative study of MRD detection by using both T cell receptor (TCR) gamma gene as gene specific marker and SIL-TAL-1 fusion gene as tumor-specific marker in one T-ALL patient.


CONCLUSIONS
The rearrangements of IgH gene, as well as TCR gene and tumor fusion gene can be used as specific markers in the detection of MRD in ALL.
Dong
S
S
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University.
Kuang
S
S
Huang
W
W
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D015139:Blotting, Southern; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005803:Genes, Immunoglobulin; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D018365:Neoplasm, Residual; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics
D004273:DNA, Neoplasm; D016692:Receptors, Antigen, T-Cell, gamma-delta