title: Modulation of lipid unsaturation and membrane fluid state in mammalian cells by stable transformation with the delta9-desaturase gene of Saccharomyces cerevisiae.
abstract: The composition and physical state of membrane lipids determine the dynamic nature of membranes, which in turn, could directly be linked to the activity of various membrane-associated cellular functions. To better understand the molecular basis of different membrane-related phenomena we established a novel strategy to alter unsaturation of mammalian cell membranes with an identical genetic background. We transfected L929 mouse fibroblastoid cells with DNA constructs containing the Delta9-fatty acid desaturase gene (Ole1) of S. cerevisiae under the control of desaturase promoters derived either from wild type or mutant strains of the dimorphic fungus H. capsulatum.
authors.affiliation: Institute of Biochemistry, BRC, Szeged, Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Gyorfy
authors.forename: I
authors.initials: I
authors.lastname: Horváth
authors.forename: G
authors.initials: G
authors.lastname: Balogh
authors.forename: A
authors.initials: A
authors.lastname: Domonkos
authors.forename: E
authors.initials: E
authors.lastname: Duda
authors.forename: B
authors.initials: B
authors.lastname: Maresca
authors.forename: L
authors.initials: L
authors.lastname: Vígh
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D004271:DNA, Fungal; D044943:Fatty Acid Desaturases / Q000235:genetics*; D050356:Lipid Metabolism; D008560:Membrane Fluidity; D051379:Mice; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D013230:Stearoyl-CoA Desaturase; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase