Structure and subcellular localization of a small RNA-binding protein from tobacco.
In this study, a cDNA encoding a small RNA-binding protein was isolated from a Nicotiana sylvestris cDNA library. The predicted protein (RGP-3) is 144 amino acid residues long, and contains a consensus sequence-type RNA binding domain (CS-RBD) of 83 amino acids and a short glycine-rich region of 15 amino acids. RGP-3 synthesized in Escherichia coli has high affinity for poly(U). Immunocytochemical analysis indicated that RGP-3 is localized in the nucleoplasm, and that RGP-1b, a related protein reported previously, is localized in the nucleolus. Possible roles of these proteins in pre-mRNA or pre-rRNA processing are discussed.
Moriguchi
K
K
Center for Gene Research, Nagoya University, Japan.
Sugita
M
M
Sugiura
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D018076:DNA, Complementary; D015723:Gene Library; D017343:Genes, Plant; D005998:Glycine; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; D010947:Plants, Toxic; D016601:RNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D013347:Subcellular Fractions / Q000378:metabolism / Q000648:ultrastructure; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
D018076:DNA, Complementary; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; C108215:RGP-3 protein, Nicotiana sylvestris; D016601:RNA-Binding Proteins; D005998:Glycine