title: Massive infiltration of basophilic cells in inflamed tissue after injection of RANTES.
abstract: Regulated upon activation normal T expressed and secreted (RANTES) is a new inducible protein member of the human C-C branch of chemokines. RANTES is a potent monocyte and lymphocyte chemoattractant and is a mediator of inflammatory responses. In these studies we found that RANTES 10 ng/50 microl chemoattracts basophilic cells in a dose-dependent manner 4 h after an intradermal injection in rat skin sites, as revealed by optic microscopy. Moreover, in biopsy specimens from rat skin injection sites histamine release was significantly higher (P < 0.05) than in controls (PBS 50 microl) after 4 h from RANTES treatment. The presence of basophilic cells in rat skin injection sites after RANTES-treatment was also confirmed by electron microscopy studies. In addition, histidine decarboxylase (HDC) mRNA was increased in rat skin sites injected with RANTES compared to sites injected with PBS (controls). Our report describes additional biological activities for RANTES, suggesting that this human chemoattractant protein may play a fundamental role in histamine and HDC generation, along with basophilic cell recruitment.
authors.affiliation: Immunology Division, University of Chieti School of Medicine, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Conti
authors.forename: M
authors.initials: M
authors.lastname: Reale
authors.forename: R C
authors.initials: RC
authors.lastname: Barbacane
authors.forename: S
authors.initials: S
authors.lastname: Frydas
authors.forename: M
authors.initials: M
authors.lastname: Felaco
authors.forename: A
authors.initials: A
authors.lastname: Grilli
authors.forename: F C
authors.initials: FC
authors.lastname: Placido
authors.forename: I
authors.initials: I
authors.lastname: Cataldo
authors.forename: C
authors.initials: C
authors.lastname: Feliciani
authors.forename: M
authors.initials: M
authors.lastname: Di Gioacchino
authors.forename: G
authors.initials: G
authors.lastname: Anogianakis
authors.forename: D
authors.initials: D
authors.lastname: Dimitriadou
authors.forename: D
authors.initials: D
authors.lastname: Vacalis
authors.forename: A
authors.initials: A
authors.lastname: Trakatellis
mesh_terms: D000818:Animals; D001491:Basophils / Q000187:drug effects*; D018946:Chemokine CCL5 / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004790:Enzyme Induction / Q000187:drug effects; D004890:Erythema / Q000139:chemically induced / Q000473:pathology; D006632:Histamine / Q000032:analysis; D006640:Histidine Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
chemical_list: D018946:Chemokine CCL5; D012333:RNA, Messenger; D011994:Recombinant Proteins; D006632:Histamine; D006640:Histidine Decarboxylase