Effect of amylin in various experimental models of gastric ulcer.
Subcutaneous administration of amylin (20-40 micrograms/kg) prevented, in a dose-dependent manner, reserpine- and serotonin-induced gastric damage, but the anti-ulcer effect was not present when lesions were induced by pylorus ligation. The protective effect of amylin was inhibited by pretreatment with capsicin as well as CGRP-(8-37), a calcitonin gene-related peptide (CGRP) and amylin receptor antagonist, and was significantly reduced by domperidone, a dopamine D2 receptor antagonist, or neostigmine, an inhibitor of acetylcholinesterase. Our data suggest that the gastroprotective activity of amylin in some experimental models of gastric ulcers involves capsaicin-sensitive fibers and CGRP receptors. Moreover, the peptide interferes, at least in part, with the dopaminergic and parasympathetic systems.
Clementi
G
G
Institute of Pharmacology, University of Catania, School Medicine, Italy.
Caruso
A
A
Cutuli
V M
VM
Prato
A
A
de Bernardis
E
E
Amico-Roxas
M
M
D000682:Amyloid / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007279:Injections, Subcutaneous; D058228:Islet Amyloid Polypeptide; D008297:Male; D008916:Miotics / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012110:Reserpine / Q000633:toxicity; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*
D000682:Amyloid; D000897:Anti-Ulcer Agents; D058228:Islet Amyloid Polypeptide; D008916:Miotics; D010446:Peptide Fragments; C058636:calcitonin gene-related peptide (8-37); D012110:Reserpine; D015740:Calcitonin Gene-Related Peptide