[Inhibitory effect of interleukin-10 on inflammatory reaction in rat mesangial cells].
OBJECTIVE
To investigate the inhibitory effect of IL-10 on inflammatory reaction in rat mesangial cells (rMC).


METHODS
Cell proliferation was tested by 3H-thymidine uptake and absolute cell counts. The production of IL-1 and TNF alpha by rMC was assessed by bioactivity assay and their gene expression by Northern blot hybridization. The expression of intercellular adhesion molecule-1 (ICAM-1) on rMC was determined by ELISA.


RESULTS
IL-10 (25 ng/ml) inhibited 5% FCS and IL-1 induced cell proliferation by 39% and 52% respectively. It also suppressed the production of IL-1 and TNF alpha bioactivity by rMC by 48% and 68%, which was consistent with the decline of IL-1 and TNF alpha gene expression. The IL-1-induced expression of ICAM-1 on surface of rMC was attenuated by IL-10 treatment.


CONCLUSIONS
Our data suggest that IL-10 may be an inhibitory cytokine in regulation of inflammatory reaction in glomerular mesangial cells.
Dai
Z
Z
Institute of Nephrology, Beijing Medical University.
Li
X
X
Wang
H
H
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000187:drug effects / Q000473:pathology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000494:pharmacology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10