title: Human papillomavirus type 18 DNA sequences in adenocarcinoma and adenosquamous carcinoma of the uterine cervix.
abstract: Human papillomavirus (HPV) DNA sequences were detected by Southern blot hybridization and polymerase chain reaction (PCR) in 10 out of 19 patients (52.7%) with adenocarcinoma [15] and adenosquamous [4] carcinoma of the uterine cervix. HPV 18 DNA was detected in 8 of these 19 patients (42.1%), HPV 16 DNA in 1 patient (5.3%) and HPV type X (unknown) in another (5.3%). Of the 10 HPV positive samples HPV 18 was found in 6 out of 6 pure adenocarcinomas (100%), and in 2 of 4 (50%) adenosquamous carcinomas. HPV 16 and HPV X were each detected in 1 out of 4 (25%) adenosquamous carcinomas. The physical state of the viral DNA was investigated in 5 of the 10 HPV-positive cases. All the specimens from these 5 cases showed HPV to be integrated into the host genome, except for one adenosquamous specimen, which showed both episomal and integrated forms of HPV 16. Six of 8 HPV 18 DNA positive specimens were from cases of pure adenocarcinoma and it was found by PCR that five of these 6 specimens retained fragments of E6/E7, LCR/E7 and early sequence of E1 fragment (sequence: 1188-1373) but deleted most part of E1.
authors.affiliation: Department of Gynecology, Cancer Institute Hospital, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Teshima
authors.forename: S
authors.initials: S
authors.lastname: Beaudenon
authors.forename: S
authors.initials: S
authors.lastname: Koi
authors.forename: K
authors.initials: K
authors.lastname: Katase
authors.forename: K
authors.initials: K
authors.lastname: Hasumi
authors.forename: K
authors.initials: K
authors.lastname: Masubuchi
authors.forename: G
authors.initials: G
authors.lastname: Orth
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000821:virology*; D001483:Base Sequence; D001706:Biopsy; D015139:Blotting, Southern; D018196:Carcinoma, Adenosquamous / Q000473:pathology / Q000821:virology*; D002584:Cervix Uteri / Q000737:chemistry / Q000473:pathology / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D015344:DNA Probes, HPV / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*
chemical_list: D017931:DNA Primers; D015344:DNA Probes, HPV; D004279:DNA, Viral