title: Efficient gene targeting in mouse embryonic stem cells.
abstract: We developed methods to improve the efficiency of gene correction in mouse embryonic stem cells using homologous recombination of a replacement vector. The absolute frequency of homologous recombination in mouse embryonic stem (ES) cells, defined as the frequency of homologous recombination per electroporated cell, is approximately 10(-5) to 10(-6) by current procedures. Our method for gene targeting in mouse ES cells produces an absolute frequency of 10(-1). The protocol uses micro-electroporation chambers and a modified electroporation procedure that does not cause significant cell death. Plating and growth of the electroporated cells at an optimum density to maintain viability significantly increased the recovery of targeted cells. Due to the high frequency of targeting, corrected cells could be isolated by screening colonies obtained after growth without selection. Alternatively, colony formation and the absolute frequency could be increased by co-plating the electroporated cells with nonelectroporated ES cells before the addition of selective medium. These parental cells were nonirradiated but were killed in the selective medium. Plating density and efficiency of colony formation are therefore critical factors for obtaining a high absolute frequency of homologous recombination. Because this frequency is extremely high, these methods can be used to perform gene targeting without the use of selectable markers.
authors.affiliation: ABL-Basic Research Program, NCI-FCRDC, Frederick, MD 21702-1201, USA.
authors.forename: N S
authors.initials: NS
authors.lastname: Templeton
authors.forename: D D
authors.initials: DD
authors.lastname: Roberts
authors.forename: B
authors.initials: B
authors.lastname: Safer
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D018274:Electroporation; D018390:Gene Targeting / Q000379:methods*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis / Q000235:genetics*; D051379:Mice; D011995:Recombination, Genetic; D013234:Stem Cells
chemical_list: D007041:Hypoxanthine Phosphoribosyltransferase