title: Complexes between nascent polypeptides and their molecular chaperones in the cytosol of mammalian cells.
abstract: Folding of newly synthesized proteins in vivo is believed to be facilitated by the cooperative interaction of a defined group of proteins known as molecular chaperones. We investigated the direct interaction of chaperones with nascent polypeptides in the cytosol of mammalian cells by multiple methods. A new approach using a polyclonal antibody to puromycin allowed us to tag and capture a population of truncated nascent polypeptides with no bias as to the identity of the bound chaperones. In addition, antibodies that recognize the cytosolic chaperones hsp70, CCT (TRiC), hsp40, p48 (Hip), and hsp90 were compared on the basis of their ability to coprecipitate nascent polypeptides, both before and after chemical cross-linking. By all three approaches, hsp70 was found to be the predominant chaperone bound to nascent polypeptides. The interaction between hsp70 and nascent polypeptides is apparently dynamic under physiological conditions but can be stabilized by depletion of ATP or by cross-linking. The cytosolic chaperonin CCT was found to bind primarily to full-length, newly synthesized actin, and tubulin. We demonstrate and caution that nascent polypeptides have a propensity for binding many proteins nonspecifically in cell lysates. Although current models of protein folding in vivo have described additional components in contact with nascent polypeptides, our data indicate that the hsp70 and, perhaps, the hsp90 families are the predominant classes of molecular chaperones that interact with the general population of cytosolic nascent polypeptides.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, USA.
authors.forename: D K
authors.initials: DK
authors.lastname: Eggers
authors.forename: W J
authors.initials: WJ
authors.lastname: Welch
authors.forename: W J
authors.initials: WJ
authors.lastname: Hansen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D018832:Molecular Chaperones / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011233:Precipitin Tests; D011691:Puromycin / Q000494:pharmacology*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D010455:Peptides; D011691:Puromycin; D010450:Endopeptidases