title: Association between cigarette smoking and lipid peroxidation in a controlled feeding study.
abstract: BACKGROUND
  Cigarette smoke may promote atherogenesis by producing oxygen-derived free radicals that damage lipids. However, evidence in support of this hypothesis is inconsistent because most studies did not control for aspects of diet (antioxidants and lipid substrate) that may confound the association between smoking and measures of lipid peroxidation.
  
  
  RESULTS
  The relationships between cigarette smoking and two measures of lipid peroxidation, breath ethane (an in vivo assay) and thiobarbituric acid-reactive substances (TBARS, an in vitro assay), were examined in 123 adults (11% of whom were smokers) participating in a controlled feeding study. After 3 weeks of controlled feeding on a common diet (36% total fat, 14% saturated fats, 6% polyunsaturated fats, and 12% monounsaturated fats), breath and fasting serum samples were collected for measurement of ethane and TBARS, respectively. Baseline characteristics of smokers and nonsmokers were similar, including several indices related to diet and nutritional status (albumin, cholesterol, body mass index, and oxygen radical-absorbing capacity). Cigarette smokers had significantly higher breath ethane (8.88 versus 1.71 pmol/L; P<.0001) and TBARS (24.0 versus 20.7 micromol/mL; P=.008) than nonsmokers. The interval between breath collection and the time the last cigarette was smoked was significantly and inversely correlated with breath ethane. Neither measure of lipid peroxidation was associated with measures of serum cholesterol or albumin, body mass index, or serum oxygen radical-absorbing capacity.
  
  
  CONCLUSIONS
  Cigarette smokers have higher rates of in vivo and in vitro lipid peroxidation. These results support the hypothesis that the atherogenic effects of smoking are mediated in part by free radical damage to lipids.
authors.affiliation: Welch Center for Prevention, Epidemiology and Clinical Research, Department of Medicine, The Johns Hopkins Medical Institutions, Baltimore, Md 21205-2223, USA. ermiller@welchlink.welch.jhu.edu
authors.forename: E R
authors.initials: ER
authors.lastname: Miller
authors.forename: L J
authors.initials: LJ
authors.lastname: Appel
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: T H
authors.initials: TH
authors.lastname: Risby
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage; D001944:Breath Tests; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage; D004980:Ethane / Q000032:analysis; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D012907:Smoking / Q000097:blood / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D004041:Dietary Fats; D017392:Thiobarbituric Acid Reactive Substances; D002784:Cholesterol; D004980:Ethane