title: Molecular characterization of a mutation affecting the amount of Streptococcus pneumoniae penicillin-binding protein 3.
abstract: We have studied the molecular structure of the gene for the penicillin binding protein (PBP 3) of the Streptococcus pneumoniae wild-type strain and a laboratory mutant strain that exhibits a reduced amount of this protein on PBP gels. This mutation affects cefotaxime resistance when transferred into resistant strains. We have sequenced the PBP3 gene, dacA, and upstream regions from the wild-type isogenic strain and the laboratory mutant. We show that a deletion of one base-pair in the upstream sequence of this gene account for the phenotype by decreasing the amount of PBP3.
authors.affiliation: Laboratoire de Microbiologie et Génétique Moléculaire du CNRS, Toulouse, France.
authors.forename: L
authors.initials: L
authors.lastname: Selakovitch-Chenu
authors.forename: P
authors.initials: P
authors.lastname: Giammarinaro
authors.forename: M
authors.initials: M
authors.lastname: Sicard
mesh_terms: D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D006602:Hexosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D002267:Muramoylpentapeptide Carboxypeptidase; D009154:Mutation; D009690:Nucleic Acid Conformation; D010403:Penicillin Resistance / Q000235:genetics; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000235:genetics*; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase