title: Saturation kinetics of coenzyme Q in NADH oxidation: rate enhancement by incorporation of excess quinone.
abstract: In beef heart mitochondria it has been found that the Km for coenzyme Q10 of the NADH oxidation system is in the range of the membrane concentration of the quinone; this is contrary to succinate oxidation which is in Vmax with respect to quinone content. The same proportional difference between the two systems is maintained in their affinities for the exogenous acceptor CoQ1 in non-extracted mitochondria. The Km of succinate- coenzyme Q reductase for CoQ1 is reversibly lowered in CoQ-depleted mitochondria; while in contrast the Km for NADH-coenzyme Q reductase is reversibly increased by CoQ extraction. Incorporation of exogenous quinones by co-sonication with submitochondrial particles, as evidenced by fluorescence quenching of pyrene, enhances NADH-cytochrome c reductase activity in accordance with the lack of saturation of the former system.
authors.affiliation: Department of Biochemistry, G. Moruzzi, University of Bologna, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Fato
authors.forename: S D
authors.initials: SD
authors.lastname: Bernardo
authors.forename: E
authors.initials: E
authors.lastname: Estornell
authors.forename: G
authors.initials: G
authors.lastname: Parentic Castelli
authors.forename: G
authors.initials: G
authors.lastname: Lenaz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D016227:Benzoquinones / Q000378:metabolism*; D002417:Cattle; D042963:Electron Transport Complex II; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D013010:Sonication; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014451:Ubiquinone / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D016227:Benzoquinones; D009097:Multienzyme Complexes; D009243:NAD; D014451:Ubiquinone; C004532:quinone; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone); D009245:NADH Dehydrogenase