title: Structure-activity relationships for some elastin-derived peptide chemoattractants.
abstract: In an attempt to explore the relationships between conformation of chemotactic peptides related to elastin and their biological activity we have studied five peptides: VGVAPG, VGVPG, VGAPG, GVAPG and GGVPG in solvents of different polarities which may mimic the environmental conditions at the receptor site. CD and NMR studies showed that GVAPG has no preference for structured conformations, while the other peptides may assume folded conformations in organic solvents. All these peptides but GGVPG showed chemotactic activity for monocytes. The chemotactic activity of VGVPG, VGAPG and VGVAPG was inhibited by lactose, while chemotaxis of peptide GVAPG was insensitive to lactose, suggesting the existence of different chemotactic receptors.
authors.affiliation: Department of Chemistry, University of Basilicata, Potenza, Italy.
authors.forename: M A
authors.initials: MA
authors.lastname: Castiglione Morelli
authors.forename: F
authors.initials: F
authors.lastname: Bisaccia
authors.forename: S
authors.initials: S
authors.lastname: Spisani
authors.forename: M
authors.initials: M
authors.lastname: De Biasi
authors.forename: S
authors.initials: S
authors.lastname: Traniello
authors.forename: A M
authors.initials: AM
authors.lastname: Tamburro
mesh_terms: D002630:Chemotactic Factors / Q000737:chemistry* / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002942:Circular Dichroism; D004549:Elastin / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology*; D011487:Protein Conformation; D013329:Structure-Activity Relationship
chemical_list: D002630:Chemotactic Factors; D009842:Oligopeptides; D004549:Elastin