Identification of human complement factor H as a chemotactic protein for monocytes.
We used chromatographic separation to purify to homogeneity a monomeric monocyte chemotactic protein of 150 kDa contained in mesothelioma pleural effusions. It was identified by N-terminal amino acid sequencing and immunoblotting as complement factor H, an inhibitor of the alternative complement pathway. Specific antibodies against factor H inhibited the monocyte chemotactic activity of the purified protein, which was most active at 10 nM. Factor H is a restrictive factor of alternative complement pathway activation. The new chemotactic function assigned to factor H in recruiting monocytes to the mesothelioma site might contribute to malignant cell phagocytosis via the iC3b/complement receptor type 3 pathway. These functions link the humoral and cellular immune systems.
Nabil
K
K
Institut National de la Santé et de la Recherche Médicale, Unité 14, CHU Brabois, Vandoeuvre Les Nancy, France.
Rihn
B
B
Jaurand
M C
MC
Vignaud
J M
JM
Ripoche
J
J
Martinet
Y
Y
Martinet
N
N
D000595:Amino Acid Sequence; D002630:Chemotactic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D002634:Chemotaxis, Leukocyte; D017242:Complement Factor H / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D008654:Mesothelioma / Q000737:chemistry; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D010996:Pleural Effusion / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism
C493495:CFH protein, human; D002630:Chemotactic Factors; D017242:Complement Factor H; D013917:Thrombin