Evaluation of IgG glycation levels by matrix-assisted laser desorption/ionization mass spectrometry.
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has been employed for the evaluation of the glycation level of IgG from healthy subjects and also from well- and badly-controlled diabetic patients. The measurements have been performed on untreated plasma protein fractions. The data obtained have shown that a clear mass increase, originating from non-enzymatic glycation processes, is observed in the case of diabetic patients: for well-controlled ones it is in the range 512-1565 Da, while it becomes 827-4270 Da for badly-controlled diabetic patients. This approach indicates that MALDI mass spectrometry is a highly specific tool that can be employed in the metabolic control of diabetic patients and in studies relating the IgG glycation level to possible immunological impairment.
Lapolla
A
A
Istituto di Medicina Interna, Malattie del Metabolismo, Padova, Italy.
Fedele
D
D
Aronica
R
R
Garbeglio
M
M
D'Alpaos
M
M
Seraglia
R
R
Traldi
P
P
D000368:Aged; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008875:Middle Aged; D012709:Serum Albumin / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
D001786:Blood Glucose; D007074:Immunoglobulin G; D012709:Serum Albumin; D005947:Glucose