Effect of amphotericin B on the lipids of yeast cells of Sporothrix schenckii.
Yeast cells of five strains of Sporothrix schenckii were obtained for partial analysis of lipid composition. Quantitative analysis of lipids and sterols were completed, as well as qualitative analysis of sterols by thin layer chromatography and by ultraviolet spectra. These determinations were made on cells cultured in the absence and presence of amphotericin B at sub-MIC (minimum inhibitory concentration) levels. Marked alterations in lipid content were observed in the amphotericin B-treated cells. The major alterations were the reduction of total lipid (18.7-57.6%) and sterols (48.5-96.7%) after exposure to the polyenic antibiotic. It is concluded that amphotericin B altered the lipid profiles, especially sterols of S. schenckii.
Hamdan
J S
JS
Department of Microbiology, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Casali
A K
AK
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D004875:Ergosterol / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008826:Microbial Sensitivity Tests; D013175:Sporothrix / Q000187:drug effects*; D013185:Squalene / Q000032:analysis
D000935:Antifungal Agents; D008055:Lipids; D013185:Squalene; D000666:Amphotericin B; D004875:Ergosterol