Analysis of phospholipid molecular species.
A method is described for analysing molecular species of glycerophospholipids. Diglycerides obtained by phospholipase C-catalysed hydrolysis of the phospholipid are separated into the diacyl- alkylacyl- and alkenylacyl- subclasses by HPLC on silicic acid. The molecular species of diacylglycerol are separated by HPLC of underivatised diglycerides on a reverse phase octadecyl-silica column.
Vecchini
A
A
Department of Biochemistry, University of Perugia, Italy.
Panagia
V
V
Binaglia
L
L
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004075:Diglycerides / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D008027:Light; D008099:Liver / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D051381:Rats; D012542:Scattering, Radiation; D013025:Glycine max / Q000737:chemistry
D004075:Diglycerides; D006017:Glycolipids; D010743:Phospholipids; C015903:glycerolglycolipids