Modulation of cytokine production from an EpiOcular corneal cell culture model in response to Staphylococcus aureus superantigen.
The present study investigated the hypothesis that Staphylococcus aureus enterotoxin B (SEB) produces epithelial cell death and releases inflammatory cytokines that produce stromal infiltration during contact lens induced peripheral ulceration. Epithelial cells were incubated with different doses of SEB for various time periods. Culture supernatants were assayed for cytokines IL-1 beta, IL-6 and chemotactic agents IL-8 and LTB4 SEB induced the production of IL-1 beta and IL-8. Epithelial cells exposed for longer periods (48 h) with low concentrations of SEB produced significantly higher levels (P < 0.02) of IL-1 beta and IL-8 (P < 0.05) compared to a 24 h exposure. SEB did not induce the production of IL-6 and LTB4.
Thakur
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Cooperative Research Centre for Eye Research and Technology, School of Optometry, University of New South Wales, Sydney, Australia.
Clegg
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Chauhan
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Willcox
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MD
D016923:Cell Death; D002470:Cell Survival; D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects* / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis*; D004768:Enterotoxins / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D013211:Staphylococcus aureus; D018089:Superantigens / Q000494:pharmacology*
D016207:Cytokines; D004768:Enterotoxins; D018089:Superantigens; C031818:enterotoxin B, staphylococcal