Enhancement of the resistance of tilapia and grass carp to experimental Aeromonas hydrophila and Edwardsiella tarda infections by several polysaccharides.
Efficacies of eleven polysaccharides including Bar (glycan extracted from Barley), curdlan, Dex (dextran sulfate), inulin, krestin, laminaran, levan, PO (glycan extracted from Pleurotus ostreatus), scleroglucan, YG (yeast glucan), and zymosan, in the protection of tilapia, Tilapia aureus P., and grass carp, Ctenopharyngodon idellus, against bacterial infections in vivo were examined. Four glycans. namely, Bar, krestin, scleroglucan, and zymosan were observed to significantly increase the survival rates of tilapia (80, 60, 70, and 60%) and grass carp (60, 70, 90, and 60%) (p < 0.05) after injection with Aeromonas hydrophila. The above mentioned four glycans were also found to raise the survival rates of tilapia (70, 60, 80, and 50%) and grass carp (50, 50, 70, and 50%) (p < 0.05) significantly after infection with Edwardsiella tarda. Moreover, Bar, curdlan, krestin, scleroglucan, and zymosan were also found to significantly increase the number of NBT-positive staining cells (p < 0.05), which might indicate that to activate non-specific phagocytes in fish is one of the antibacterial mechanisms of polysaccharides.
Wang
W S
WS
Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
Wang
D H
DH
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D016980:Aeromonas hydrophila / Q000276:immunology* / Q000472:pathogenicity; D000818:Animals; D002347:Carps / Q000276:immunology* / Q000382:microbiology; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control; D006090:Gram-Negative Bacteria / Q000276:immunology* / Q000472:pathogenicity; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000378:metabolism; D017210:Tilapia / Q000276:immunology* / Q000382:microbiology
D000276:Adjuvants, Immunologic; D011134:Polysaccharides; D013481:Superoxides; D009580:Nitroblue Tetrazolium