Purification and antifungal activity of a basic 34 kDa beta-1,3-glucanase from soybean hypocotyls inoculated with Phytophthora sojae f. sp. glycines.
Inoculation of soybean (Glycine max L. cv. Jangyup) hypocotyls with Phytophthora sojae f. sp. glycines results in a marked accumulation of some pathogenesis-related (PR) proteins. A basic beta-1,3-glucanase (34 kDa) was purified from soybean hypocotyls infected by an incompatible race of P. sojae f. sp. glycines using CM-cellulose cation exchange chromatography and Bio-gel P-60 gel filtration. The purified soybean beta-1,3-glucanase cross-reacted with polyclonal antibody raised against a tomato beta-1,3-glucanase. The activity of beta-1,3-glucanase was much higher in the infected soybean hypocotyls than the healthy ones. The beta-1, 3-glucanase purified from soybean inhibited spore germination and hyphal growth of the chitin-negative fungus P. sojae f. sp. glycines, but did not show any antifungal activity against the chitin-containing fungi Alternaria mali, Colletotrichum gloeosporioides, and Magnaporthe grisea.
Yi
S Y
SY
Department of Agricultural Biology, Korea University, Seoul, Korea.
Hwang
B K
BK
D000528:Alternaria / Q000187:drug effects; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D043326:Glucan 1,3-beta-Glucosidase; D018546:Hypocotyl / Q000201:enzymology / Q000382:microbiology; D007120:Immunochemistry; D018551:Solanum lycopersicum / Q000201:enzymology; D003904:Mitosporic Fungi / Q000187:drug effects; D010838:Phytophthora / Q000187:drug effects* / Q000472:pathogenicity*; D012995:Solubility; D013025:Glycine max / Q000201:enzymology* / Q000382:microbiology*; D013172:Spores, Fungal / Q000187:drug effects; D001617:beta-Glucosidase / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D000935:Antifungal Agents; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase