Singlet oxygen is an early intermediate in cytokine-dependent ultraviolet-A induction of interstitial collagenase in human dermal fibroblasts in vitro.
Ultraviolet (UV) A irradiation of human dermal fibroblasts elicits an increase in specific mRNA amounts and bioactivities of the cytokines IL-1alpha, IL-1beta, and IL-6. These effects are enhanced in deuterium oxide-based medium and are diminished in the presence of non-toxic concentrations of sodium azide. Furthermore, generating singlet oxygen outside the cells by irradiation of rose bengal-coated resin particles with visible light (lambda > 450 nm) results in the induction of interstitial collagenase, IL-1 and IL-6, similar to the response observed with UVA irradiation. These observations suggest that singlet oxygen is an early intermediate in the signaling pathway of IL-1 and IL-6 mediating UVA induction of interstitial collagenase (E.C. 3.4.24.7). Furthermore, singlet oxygen appears to initiate this complex UV response at the cell membrane.
Wlaschek
M
M
Department of Dermatology, University of Cologne, Germany.
Wenk
J
J
Brenneisen
P
P
Briviba
K
K
Schwarz
A
A
Sies
H
H
Scharffetter-Kochanek
K
K
D001386:Azides / Q000494:pharmacology; D002478:Cells, Cultured; D017364:Collagenases / Q000235:genetics*; D005347:Fibroblasts / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D007378:Interleukins / Q000235:genetics* / Q000378:metabolism; D020781:Matrix Metalloproteinase 1; D009284:Naphthols / Q000494:pharmacology; D010100:Oxygen / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D012395:Rose Bengal / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology; D026082:Singlet Oxygen; D012867:Skin / Q000166:cytology; D019810:Sodium Azide; D014466:Ultraviolet Rays
D001386:Azides; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D009284:Naphthols; D012333:RNA, Messenger; D026082:Singlet Oxygen; D012395:Rose Bengal; D019810:Sodium Azide; C058008:3,3'-(1,4-naphthylidene)diproprionate; D017364:Collagenases; D020781:Matrix Metalloproteinase 1; D010100:Oxygen