Immunohistochemical localization of low-affinity nerve growth factor receptor in the enteric nervous system of adult rats.
The localization of low-affinity nerve growth factor receptor in the enteric nervous system of adult rats has been studied by immunohistochemistry using a monoclonal antibody (clone 192) against the rat receptor. Cryostat and whole-mount sections were stained. By light and confocal microscopy, positive staining in neural structures was found in every part of the gut. In the ganglionic plexus, dense staining was detected in the neuropil surrounding neuronal cell bodies that were themselves devoid of immunoreactivity. Immunoelectron microscopy revealed deposition of reaction products on the outer plasma membranes of both perikarya and processes of neuronal as well as glial cells. Such a selective localization of the receptor in the plasma membrane, but not the cytoplasm, suggests that the mechanisms of receptor-ligand interaction in the gut may differ from those in the brain, where internalization of the receptor is observed in cholinergic cells. The present study provides the morphological basis for future studies designed to elucidate the functional significance of this enteric nervous system receptor. Since it is found in both neuronal and glial cells, it is probably under the influence of a number of trophic factors, including nerve growth factor.
Aimi
Y
Y
Institute of Molecular Neurobiology, Shiga University of Medical Science, Otsu, Japan.
Wakabayashi
Y
Y
Yasuhara
O
O
Matsuo
A
A
Kwok
Y N
YN
McGeer
P L
PL
Kimura
H
H
D000818:Animals; D017615:Enteric Nervous System / Q000737:chemistry*; D008297:Male; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar; D017475:Receptors, Nerve Growth Factor / Q000032:analysis*
D017475:Receptors, Nerve Growth Factor