title: Inhibition of tyrosine hydroxylase by bulbocapnine.
abstract: The inhibitory effects of bulbocapnine, an aporphine isoquinoline alkaloid, on bovine adrenal tyrosine hydroxylase (TH) were investigated. Bulbocapnine had an inhibitory effect on the enzyme (43.6% inhibition at concentration of 200 microM). Bulbocapnine exhibited uncompetitive inhibition on bovine adrenal TH with a substrate L-tyrosine. The Ki value was found to be 0.20 mM.
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhang
authors.forename: J S
authors.initials: JS
authors.lastname: Shin
authors.forename: S S
authors.initials: SS
authors.lastname: Lee
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: M K
authors.initials: MK
authors.lastname: Lee
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D001060:Aporphines / Q000494:pharmacology*; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D016716:PC12 Cells; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors*
chemical_list: D001060:Aporphines; D004791:Enzyme Inhibitors; D014446:Tyrosine 3-Monooxygenase; C083807:bulbocapnine