Induction of uncoupling protein-2 mRNA by troglitazone in the pancreatic islets of Zucker diabetic fatty rats.
Because troglitazone, like leptin, lowers the triglyceride (TG) content of pancreatic islets, we searched for other leptinomimetic actions. Leptin upregulates the expression of uncoupling protein-2 (UCP-2) mRNA in islets of normal rats, but has no effect in islets of obese Zucker Diabetic Fatty (ZDF) rats with mutated leptin receptors. We report here that troglitazone also increases the UCP-2/beta-actin mRNA ratio by 115% in wild type ZDF rats and by 400% in obese ZDF rats.
Shimabukuro
M
M
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, 75235, USA.
Zhou
Y T
YT
Lee
Y
Y
Unger
R H
RH
D000818:Animals; D002839:Chromans / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D007473:Ion Channels; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D009765:Obesity / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D011924:Rats, Zucker; D013844:Thiazoles / Q000494:pharmacology*; D045162:Thiazolidinediones; D000077288:Troglitazone; D000071246:Uncoupling Protein 2; D015854:Up-Regulation / Q000187:drug effects
D002839:Chromans; D007004:Hypoglycemic Agents; D007473:Ion Channels; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D012333:RNA, Messenger; D013844:Thiazoles; D045162:Thiazolidinediones; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; D000077288:Troglitazone