Catfish thrombocytes express an integrin-like CD41/CD61 complex.
A thrombocyte-specific antigen was identified in two closely related catfish, Ictalurus punctatus and Ictalurus furcatus, by monoclonal antibodies 4-20 and 7-2. The antibodies immunoprecipitate two noncovalently associated glycoprotein chains of Mr 180,000 and Mr 95,000. Under reducing conditions the Mr 180,000 chain is resolved into Mr 150,000 and 32,000 subcomponents. Analysis of N-terminal amino acid sequences indicates homology of the Mr 95,000 chain with the beta3 integrin subunit and homology of the Mr 150,000 chain with the alphaIIb integrin subunit. These antibodies induce catfish thrombocyte aggregation and alteration of cell shape. The data indicate conservation of the megakaryocyte/platelet-restricted CD41/CD61 complex in bony fish.
Passer
B J
BJ
Department of Microbiology, University of Alabama at Birmingham, 35294, USA.
Chen
C H
CH
Miller
N W
NW
Cooper
M D
MD
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis* / Q000737:chemistry; D000954:Antigens, Surface / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry*; D006031:Glycosylation; D007059:Ictaluridae / Q000097:blood*; D039661:Integrin beta3; D016023:Integrins / Q000097:blood* / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015539:Platelet Activation; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis* / Q000737:chemistry; D010980:Platelet Membrane Glycoproteins / Q000032:analysis* / Q000737:chemistry; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D039661:Integrin beta3; D016023:Integrins; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins