Binding of nascent glucuronoxylan to the cell walls of pea seedlings.
Glucuronoxylan synthesised in vitro by membrane-bound enzymes from etiolated pea epicotyls was found to bind to isolated cell walls from the same tissue in a pH-dependant manner. The binding was maximum at pH 3.5-4.0, and decreased to zero at pH 6. The bound glucuronoxylan could be dissociated from the cell walls by washing at pH 6, and the binding appeared to be non-covalent. Extraction experiments indicated that the glucuronoxylan was binding to hemicellulose in the cell-wall. The observed binding may be significant in the process of cell-wall assembly in vivo.
Brett
C T
CT
Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, UK. c.brett@bio.gla.ac.uk
Healy
S A
SA
McDonald
M S
MS
Macgregor
C
C
Baydoun
E A
EA
D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004492:Edetic Acid / Q000494:pharmacology; D019286:Endopeptidase K / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D018532:Pisum sativum / Q000166:cytology / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism; D014540:Uridine Diphosphate Xylose / Q000378:metabolism; D014990:Xylans / Q000737:chemistry / Q000378:metabolism*
D002250:Carbon Radioisotopes; D011134:Polysaccharides; D014990:Xylans; D014535:Uridine Diphosphate Glucuronic Acid; D014540:Uridine Diphosphate Xylose; C038910:glucuronoxylan; D004492:Edetic Acid; D019286:Endopeptidase K