DNA binding studies and influence on the activity of DNA topoisomerases of bis-netropsins: different effects of analogs containing cis and trans ethylene linkers.
Binding to DNA and synthetic duplex polymers of two bifunctional netropsins and effects on supercoiled plasmid DNA as well as their inhibitory potency on DNA topoisomerases have been investigated. Characteristic differences were found in the DNA binding properties of the two bis-netropsins containing a cis and trans tether as reflected by CD, thermal melting and sedimentation measurements. CD results indicate, that the bis-netropsins interact with DNA by a two-step binding mode depending on the ligand concentration. The trans bis-netropsin may form stable complexes with different DNA's at high salt concentration, whereas for cis bis-netropsin DNA complexes the second binding step is completely abolished. The variations in the DNA binding ability of trans and cis bis-netropsin show a close relationship to the differences observed in their inhibitory effects on DNA topoisomerases. It appeared that trans bis-netropsin more strongly blocks topoisomerase activity than the cis isomer and represents the most potent inhibitor of DNA gyrase. Differences in the DNA. binding ability of the bis-netropsins and their inhibitory potency on topoisomerase activity are explained in terms of bidentate and monodentate binding mode of the trans and cis isomer, respectively.
Burckhardt
G
G
Abteilung Molekulare Biologie, Institut für Molekularbiologie der Friedrich-Schiller-Universität, Jena, Germany.
Simon
H
H
Störl
K
K
Triebel
H
H
Walter
A
A
Lown
J W
JW
Zimmer
C
C
D001667:Binding, Competitive; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004264:DNA Topoisomerases, Type I / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry; D005030:Ethylenes / Q000737:chemistry*; D009429:Netropsin / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D013237:Stereoisomerism
D004278:DNA, Superhelical; D005030:Ethylenes; D009429:Netropsin; D004247:DNA; C036216:ethylene; D004264:DNA Topoisomerases, Type I