title: Characterization of the genome of feline foamy virus and its proteins shows distinct features different from those of primate spumaviruses.
abstract: The genome of the feline foamy virus (FeFV) isolate FUV was characterized by molecular cloning and nucleotide sequence analysis of subgenomic proviral DNA. The overall genetic organization of FeFV and protein sequence comparisons of different FeFV genes with their counterparts from other known foamy viruses confirm that FeFV is a complex foamy virus. However, significant differences exist when FeFV is compared with primate foamy viruses. The FeFV Gag protein is smaller than that of the primate spumaviruses, mainly due to additional MA/CA sequences characteristic of the primate viruses only. Gag protein sequence motifs of the NC domain of primate foamy viruses assumed to be involved in genome encapsidation are not conserved in FeFV. FeFV Gag and Pol proteins were detected with monospecific antisera directed against Gag and Pol domains of the human foamy virus and with antisera from naturally infected cats. Proteolytic processing of the FeFV Gag precursor was incomplete, whereas more efficient proteolytic cleavage of the pre125Pro-Pol protein was observed. The active center of the FeFV protease contains a Gln that replaces an invariant Gly residue at this position in other retroviral proteases. Functional studies on FeFV gene expression directed by the promoter of the long terminal repeat showed that FeFV gene expression was strongly activated by the Bell/Tas transactivator protein. The FeFV Bell/Tas transactivator is about one-third smaller than its counterpart of primate spumaviruses. This difference is also reflected by a limited sequence similarity and only a moderate conservation of structural motifs of the different foamy virus transactivators analyzed.
authors.affiliation: Abteilung Retrovirale Genexpression, Forschungsschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Winkler
authors.forename: J
authors.initials: J
authors.lastname: Bodem
authors.forename: L
authors.initials: L
authors.lastname: Haas
authors.forename: M
authors.initials: M
authors.lastname: Zemba
authors.forename: H
authors.initials: H
authors.lastname: Delius
authors.forename: R
authors.initials: R
authors.lastname: Flower
authors.forename: R M
authors.initials: RM
authors.lastname: Flügel
authors.forename: M
authors.initials: M
authors.lastname: Löchelt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002415:Cats; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000235:genetics; D015686:Gene Products, env / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics; D015684:Gene Products, pol / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011323:Primates; D011533:Proviruses / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012191:Retroviridae Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D016092:Spumavirus / Q000235:genetics* / Q000302:isolation & purification; D015534:Trans-Activators / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D015686:Gene Products, env; D015683:Gene Products, gag; D015684:Gene Products, pol; D012191:Retroviridae Proteins; D015534:Trans-Activators; D014764:Viral Proteins; C074061:bel1 protein, Human foamy virus