title: Pervanadate elicits proliferation and mediates activation of mitogen-activated protein (MAP) kinase in the nucleus.
abstract: There is growing evidence for the role of protein tyrosine phosphatases in controlling such fundamental cellular processes as growth and differentiation. Pervanadate is a potent inhibitor of protein tyrosine phosphatase which has been observed here to induce proliferation in C3H10T1/2 mouse fibroblasts. Pervanadate also translocated/activated p42/44 mitogen-activated protein (MAP) kinase to the cell nucleus. An almost similar pattern of nuclear p42/44 MAP kinase stimulation is seen with TPA. On the other hand, TPA treatment results in a rapid activation of cytosolic MAP kinase which declines with time. Thus pervanadate appears as a very useful tool for studying tyrosine phosphorylation.
authors.affiliation: Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, UPR 416 du CNRS, Strasbourg, France.
authors.forename: M M
authors.initials: MM
authors.lastname: Krady
authors.forename: S
authors.initials: S
authors.lastname: Freyermuth
authors.forename: P
authors.initials: P
authors.lastname: Rogue
authors.forename: A N
authors.initials: AN
authors.lastname: Malviya
mesh_terms: D000818:Animals; D001692:Biological Transport; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology*; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008808:Mice, Inbred CBA; D014638:Vanadates / Q000494:pharmacology*
chemical_list: C076715:pervanadate; D014638:Vanadates; D017871:Calcium-Calmodulin-Dependent Protein Kinases