[Method of estimation of lysozyme activity in serum with chitin azure as substrate].
The method of estimation of lysozyme activity in serum when chitin azure is used as substrate is described. The basis of incubation medium is 0.1 M acetate buffer, pH 5.0. It was judged about activity of lysozyme by fluorescence of azure in incubation medium at the end of three hour incubation at 37 degrees C after separation on non-reacted chitin azure by short time centrifugation. It was shown that development of wound infection is accompanied by increasing of chitinase activity of lysozyme in serum.
Pisarzhevskií
S A
SA
Institute of Surgery of AV Vishnevskií RAMN, Moscow.
Globa
A G
AG
Shirshov
O N
ON
Marchuk
A I
AI
Svetukhin
A M
AM
Karelin
A A
AA
D001399:Azure Stains; D015415:Biomarkers / Q000097:blood; D002686:Chitin; D006801:Humans; D009113:Muramidase / Q000097:blood*; D013050:Spectrometry, Fluorescence; D014946:Wound Infection / Q000201:enzymology
D001399:Azure Stains; D015415:Biomarkers; D002686:Chitin; D009113:Muramidase