title: High dietary omega-6 polyunsaturated fatty acids drastically increase the formation of etheno-DNA base adducts in white blood cells of female subjects.
abstract: Lipid peroxidation generates reactive aldehydes such as trans-4-hydroxy-2-nonenal and malonaldehyde, which form promutagenic exocyclic DNA adducts in human cells and may contribute to diet-related cancers. Using ultrasensitive detection methods, analysis of WBC DNA from volunteers in a dietary study revealed that high intake of omega-6 polyunsaturated fatty acids increased malonaldehyde-derived adducts in male and female subjects. In contrast, etheno adducts (1,N6-ethenodeoxyadenosine; 3,N4-ethenodeoxycytidine) were not elevated in males but were, on average, 40 times higher in females, displaying a huge intersubject variation in lipid peroxidation-derived DNA damage. Exocyclic DNA adducts are promising biomarkers for examining the hypothesis of possible links between increased intake of dietary omega-6 polyunsaturated fatty acids, DNA damage, and elevated cancer risk for breast, colon, and prostate.
authors.affiliation: Division of Toxicology and Cancer Risk Factors, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Nair
authors.forename: C E
authors.initials: CE
authors.lastname: Vaca
authors.forename: I
authors.initials: I
authors.lastname: Velic
authors.forename: M
authors.initials: M
authors.lastname: Mutanen
authors.forename: L M
authors.initials: LM
authors.lastname: Valsta
authors.forename: H
authors.initials: H
authors.lastname: Bartsch
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D018736:DNA Adducts / Q000097:blood*; D003839:Deoxyadenosines / Q000097:blood*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000097:blood; D004042:Dietary Fats, Unsaturated / Q000009:adverse effects*; D005229:Fatty Acids, Monounsaturated; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000009:adverse effects*; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D007962:Leukocytes; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000209:etiology*; D010938:Plant Oils / Q000008:administration & dosage; D000074262:Rapeseed Oil; D012307:Risk Factors; D012737:Sex Factors; D013329:Structure-Activity Relationship; D000074242:Sunflower Oil
chemical_list: D014408:Biomarkers, Tumor; D018736:DNA Adducts; D003839:Deoxyadenosines; D004042:Dietary Fats, Unsaturated; D005229:Fatty Acids, Monounsaturated; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; D000074242:Sunflower Oil; D003841:Deoxycytidine; C032221:1,N(6)-ethenodeoxyadenosine; C032222:3,N(4)-ethenodeoxycytidine