Rates of mitochondrial and peroxisomal beta-oxidation of palmitate change during postnatal development and food deprivation in liver, kidney and heart of pigs.
We measured total, mitochondrial and peroxisomal capacities for beta-oxidation of [1-14C]palmitate in homogenates of liver, kidney and heart from pigs within 0.5 h after birth (0 h, unfed) and at 24 h (suckled or unfed), 10 d (suckled or 24-h food-deprived), 21 d (suckled or 24-h food-deprived) and 5 mo (overnight food-deprived) of age. Assays were conducted in the absence (total beta-oxidation) or presence (peroxisomal beta-oxidation) of antimycin A and rotenone. Mitochondrial beta-oxidation was calculated as total minus peroxisomal beta-oxidation. Acid-soluble products (ASP) from incubation of tissue homogenates from 24-h-old unfed pigs with [1-14C]palmitate were analyzed by radio-HPLC. Total and mitochondrial beta-oxidation capacities were greater (P < 0.05) at 24 h after birth in liver, and at 10 d in kidney and heart, than at 0 or 24 h. Peroxisomal beta-oxidation capacity was increased (P < 0. 05) at 24 h after birth in liver and at 10 and 21 d in heart; in kidney, the capacity was higher during the preweaning period than in adults. Across ages, peroxisomal beta-oxidation capacity represented 37 to 51%, 28 to 41%, and 26 to 31% of total beta-oxidation capacity in liver, kidney, and heart, respectively. Food deprivation increased hepatic total beta-oxidation at 10 d and decreased peroxisomal beta-oxidation at 24 h but had no effect in kidney and heart. Regardless of the presence of respiratory inhibitors, 32%, 31 to 40%, and 45 to 50% of palmitate carboxyl carbon in acid-soluble products was accumulated in acetate in liver, kidney, and heart, respectively. We suggest that a high percentage contribution of peroxisomal beta-oxidation may act as a compensatory mechanism for piglets to oxidize milk fatty acids during postnatal development. Furthermore, acetogenesis may be an important fate of acetyl-CoA from beta-oxidation of fatty acids in various piglet tissues.
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Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Drackley
J K
JK
Odle
J
J
D000818:Animals; D000831:Animals, Newborn; D002245:Carbon Dioxide / Q000378:metabolism; D005508:Food Deprivation / Q000502:physiology*; D006321:Heart / Q000254:growth & development; D007668:Kidney / Q000254:growth & development / Q000378:metabolism*; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D008830:Microbodies / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010168:Palmitates / Q000378:metabolism*; D013552:Swine
D010168:Palmitates; D002245:Carbon Dioxide