The influence of prostaglandin E1 on in vitro transcription of sperm chromatin from healthy males.
Run-on transcription experiments with sonicated chromatin from healthy individuals in the presence and absence of prostaglandin E1 showed different levels of transcription. Thus the level of incorporation of 14C-UTP into pre-mRNA proved to be about twice as high in the presence of postaglandin E1 as in the controls. The kinetic data of the in vitro transcription indicated that there were two peaks of incorporation of 14C-UTP into RNA both in the controls and in prostaglandin E1 treated probes but at different time intervals. The results show that prostaglandin E1 has a stimulatory effect on in vitro transcription of chromatin, isolated from normal patients.
Pironcheva
G L
GL
Institute of Molecular Biology, Bulgarian Academy of Science, Sofia, Bulgaria.
Miteva
K
K
Russev
G
G
Zlatarev
S
S
D000527:Alprostadil / Q000494:pharmacology*; D002250:Carbon Radioisotopes; D002843:Chromatin / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D012322:RNA Precursors / Q000096:biosynthesis / Q000378:metabolism; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014544:Uridine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology*
D002250:Carbon Radioisotopes; D002843:Chromatin; D012322:RNA Precursors; D014665:Vasodilator Agents; D000527:Alprostadil; D014544:Uridine Triphosphate