title: Dual modulation of Na/H antiport by atrial natriuretic factor in rat aortic smooth muscle cells.
abstract: The aim of the present work was to study the effect of the atrial natriuretic factor (ANF) on the Na/H antiport in rat aorta smooth muscle cells, evaluated as intracellular pH (pHi) recovery after an acid load with ammonium chloride. The Na/H antiport was studied using a fluorescent probe, sensitive to pHi, 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein. Our data indicate that ANF modulates the activity of the Na/H antiport in both a dose- and time-dependent manner. Hormone concentrations of 10(-10) M activate the antiport, increasing both the rate of recovery and the set point by approximately 0.2 pH units. This effect is mediated by diacylglycerol as a result of phospholipid hydrolysis by a phospholipase C, even if an involvement of adenosine 3',5'-cyclic monophosphate (cAMP) cannot be ruled out. ANF (10(-7) M) inhibits the antiport, decreasing both the rate of recovery and the set point by approximately 0.3 pH units, because of guanosine 3',5'-cyclic monophosphate production. Both inhibition and stimulation of pHi by ANF were more pronounced when the hormone was given before the acid load, perhaps because of the longer time exposure. We present new hypotheses on the mechanism of action of this paracrine/autocrine factor.
authors.affiliation: Department of Biology, University of Rome Tor Vergata, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Ricci
authors.forename: P
authors.initials: P
authors.lastname: Baldini
authors.forename: L
authors.initials: L
authors.lastname: Bogetto
authors.forename: P
authors.initials: P
authors.lastname: De Vito
authors.forename: P
authors.initials: P
authors.lastname: Luly
authors.forename: A
authors.initials: A
authors.lastname: Zannetti
authors.forename: S
authors.initials: S
authors.lastname: Incerpi
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002021:Buffers; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000502:physiology; D004075:Diglycerides / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*
chemical_list: D002021:Buffers; D004075:Diglycerides; D010446:Peptide Fragments; D017923:Sodium-Hydrogen Exchangers; C472610:atrial natriuretic peptide, rat; D000643:Ammonium Chloride; C065367:atrial natriuretic factor (4-23)NH2, de-Gln(18)-de-Ser(19)-de-Gly(20,22)-de-Leu(21)-; D009320:Atrial Natriuretic Factor; D011493:Protein Kinase C; D006152:Cyclic GMP