title: Molecular characteristics of malignant lymphomas in cats naturally infected with feline immunodeficiency virus.
abstract: Neoplastic disease, especially malignant lymphomas, are often observed in cats infected with feline immunodeficiency virus (FIV). In order to clarify the characteristics of lymphoma cells and to investigate the pathogenesis in FIV-infected cats, we examined the lymphoma tissues developed in five cats naturally infected with FIV by Southern blot analyses using feline immunoglobulin (Ig), T-cell receptors (TCR) and FIV probes. All of the five cases were serologically positive for anti-FIV antibody and negative for feline leukemia virus antigen. Of these five lymphoma samples, two displayed rearrangement of the Ig heavy chain gene and deletion of the Ig light (kappa) chain gene, indicating that the tumor cells were committed to B-cell development. One tumor sample was identified as a T-cell lymphoma because of the presence of a rearranged TCR beta-chain gene. The other two cases were considered to be non-T non-B cell lymphoma because they did not show any rearrangement of the Ig and TCR genes. Therefore, no consistent tumor type was found in lymphoma cases infected with FIV. Clonal integration of FIV provirus was not detected in any of the five lymphoma samples obtained from FIV-infected cats using Southern blot analysis, although FIV proviral genome was detected in the genomic DNA of all the lymphoma samples by using a polymerase chain reaction (PCR). These results indicated that FIV might not play a direct role in tumorigenesis of lymphoma in cats.
authors.affiliation: Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan. aa47108@hongo.ecc.utokyo.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Endo
authors.forename: K W
authors.initials: KW
authors.lastname: Cho
authors.forename: K
authors.initials: K
authors.lastname: Nishigaki
authors.forename: Y
authors.initials: Y
authors.lastname: Momoi
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimura
authors.forename: T
authors.initials: T
authors.lastname: Mizuno
authors.forename: Y
authors.initials: Y
authors.lastname: Goto
authors.forename: T
authors.initials: T
authors.lastname: Watari
authors.forename: H
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002371:Cat Diseases / Q000235:genetics / Q000821:virology*; D002415:Cats; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D016089:Immunodeficiency Virus, Feline / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D016180:Lentivirus Infections / Q000235:genetics* / Q000662:veterinary* / Q000821:virology; D008223:Lymphoma / Q000235:genetics* / Q000662:veterinary* / Q000821:virology; D008297:Male; D011533:Proviruses / Q000235:genetics; D016662:Virus Integration / Q000276:immunology