Pressure-induced apoptosis in human lymphoblasts.
The viability of human lymphoblasts was investigated after compression at 37 degrees C for 30 min. Immediately after decompression, necrotic cells were observed and the viability decreased sigmoidally to 0 with increasing pressure from 85 to 200 MPa. Apoptotic cells began to be observed from 8 h after decompression in the case of compression at 100 MPa for 30 min at 37 degrees C. The apoptosis was confirmed to be pressure-induced by the Tunel method and by optical and electron microscopy. Cell death by both necrosis and apoptosis due to compression was accelerated in the S phase of the cell cycle.
Takano
K J
KJ
Institute of Materials Science, University of Tsukuba, Tsukuba, Japan. takano@mat.ims.tsukuba.ac.jp
Takano
T
T
Yamanouchi
Y
Y
Satou
T
T
D017209:Apoptosis; D002470:Cell Survival; D002478:Cells, Cultured; D002843:Chromatin / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis; D053938:DNA Fragmentation; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009336:Necrosis; D009685:Nuclear Envelope / Q000648:ultrastructure; D011312:Pressure; D013997:Time Factors
D002843:Chromatin; D004247:DNA