Diagnosis of brucellosis by using blood cultures.
The performances of three blood culture systems, Hemoline performance diphasic medium (bioMérieux, Marcy l'Etoile, France), Bactec Plus Aerobic/F* (Becton Dickinson, Paramus, N.J.), and Vital Aer (bioMérieux), were compared for the diagnosis of 17 cases of brucellosis. By using a 5-day incubation protocol, positive results were 52.9, 82.4, and 11.8%, respectively. When the protocol was extended to 7 days, the results were 76.5, 94.1, and 47.1%, respectively. Bactec was the fastest system (P < 0.05).
Ruiz
J
J
Department of Microbiology, University Hospital Virgen de la Arrixaca, Murcia, Spain. micro@isid.es
Lorente
I
I
Pérez
J
J
Simarro
E
E
Martínez-Campos
L
L
D001431:Bacteriological Techniques; D001769:Blood / Q000378:metabolism*; D002002:Brucella / Q000254:growth & development*; D002006:Brucellosis / Q000175:diagnosis*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D013997:Time Factors
D003470:Culture Media