title: Evidence for the involvement of the Gli gene family in embryonic mouse lung development.
abstract: Murine Gli, Gli2, and Gli3 are zinc finger genes related to Drosophila cubitus interuptus, a component of the hedgehog signal transduction pathway. In the embryonic lung, all three Gli genes are strongly expressed at the pseudoglandular stage, in distinct but overlapping domains of the mesoderm. Expression of Gli and Gli3, but not of Gli2, is subsequently downregulated at the canalicular stage, coincident with a decline in the expression of sonic hedgehog (Shh) and the hedgehog receptor gene, patched (Ptc). Overexpression of Shh in the lung results in increased levels of Ptc mRNA. Gli, but not Gli2, is also upregulated, suggesting a differential involvement of the Gli genes in the regulation of Ptc by SHH during lung development. Gli3 is not upregulated by Shh overexpression. However, its importance for lung development is shown by the finding that Gli3XtJ embryos, homozygous for a mutation involving a deletion of the Gli3 gene, have a stereotypic pattern of abnormalities in lung morphogenesis. The pulmonary defects in these embryos, consisting of localized shape changes and size reductions, correlate with normal Gli3 expression. Thus, our data indicate that one of the Gli genes, Gli3, is essential for normal lung development, and that another, Gli, can be placed downstream of Shh signaling in the lung.
authors.affiliation: Department of Cell Biology, Vanderbilt University Medical Center, Nashville, Tennessee, 37232-2175, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Grindley
authors.forename: S
authors.initials: S
authors.lastname: Bellusci
authors.forename: D
authors.initials: D
authors.lastname: Perkins
authors.forename: B L
authors.initials: BL
authors.lastname: Hogan
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051741:Kruppel-Like Transcription Factors; D008168:Lung / Q000002:abnormalities / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D005810:Multigene Family; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012097:Repressor Proteins; D014034:Toes / Q000002:abnormalities; D015534:Trans-Activators; D014157:Transcription Factors / Q000096:biosynthesis*; D029867:Xenopus Proteins; D000071676:Zinc Finger Protein GLI1; D000075746:Zinc Finger Protein Gli2; D000074290:Zinc Finger Protein Gli3; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; C108003:GLI3 protein, Xenopus; C065683:GLI3 protein, human; C000618551:Gli2 protein, mouse; C465571:Gli3 protein, mouse; D051741:Kruppel-Like Transcription Factors; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D029867:Xenopus Proteins; D000071676:Zinc Finger Protein GLI1; D000075746:Zinc Finger Protein Gli2; D000074290:Zinc Finger Protein Gli3