Peptide bank generated by large-scale preparation of circulating human peptides.
Human hemofiltrate (HF) is a source for the purification of circulating regulatory peptides. HF is obtained in large quantities during treatment of patients suffering from chronic renal failure. We have developed a large-scale method for separating peptides from amounts up to 10,000 1 HF into 300 fractions in a standardized two-step procedure, employing cation-exchange separation, followed by reversed-phase chromatography. These fractions represent a peptide bank containing bioactive, desalted and lyophilized peptides of blood. Screening for and isolation of regulatory human peptides is simplified by using this peptide bank.
Schulz-Knappe
P
P
Niedersächsisches Institut für Peptid-Forschung GmbH, Hannover, Germany.
Schrader
M
M
Ständker
L
L
Richter
R
R
Hess
R
R
Jürgens
M
M
Forssmann
W G
WG
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D016208:Databases, Factual; D006440:Hemofiltration; D006801:Humans; D007676:Kidney Failure, Chronic / Q000652:urine; D010455:Peptides / Q000097:blood* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides