Proteolysis and DNA replication: the CDC34 requirement in the Xenopus egg cell cycle.
The cell division cycle gene, CDC34, is required for ubiquitin-mediated degradation of G1 regulators and cell cycle progression through the transition from G1 to S phase in budding yeast. A CDC34 requirement for S phase onset in higher eukaryotes has not been established. Studies of the simple embryonic cell cycle of Xenopus laevis eggs demonstrated that Cdc34p in a large molecular size complex was required in the initiation of DNA replication. Cdc34p appears to regulate the initiation function of Cdk2-cyclin E, perhaps through the degradation of the Xenopus cdk inhibitor, Xic1.
Yew
P R
PR
Department of Cell Biology, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA.
Kirschner
M W
MW
D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D042846:CDC2-CDC28 Kinases; D018797:Cell Cycle Proteins; D002467:Cell Nucleus / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016213:Cyclins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004261:DNA Replication; D004791:Enzyme Inhibitors / Q000378:metabolism; D006801:Humans; D008025:Ligases / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008938:Mitosis; D009154:Mutation; D010063:Ovum / Q000166:cytology / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011506:Proteins; D011994:Recombinant Proteins / Q000378:metabolism; D016196:S Phase; D025521:Tumor Suppressor Proteins; D044763:Ubiquitin-Conjugating Enzymes; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases; D029867:Xenopus Proteins; D014982:Xenopus laevis
D018797:Cell Cycle Proteins; D016213:Cyclins; D004791:Enzyme Inhibitors; D008869:Microtubule-Associated Proteins; D011506:Proteins; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; D029867:Xenopus Proteins; C493963:Xicl protein, Xenopus; D050760:Cyclin-Dependent Kinase Inhibitor p27; C577447:CDC34 protein, human; D044763:Ubiquitin-Conjugating Enzymes; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; C495891:Cdk2 protein, Xenopus; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; D008025:Ligases