[Effect of alpha-tocopherol and nuclear tocopherol-binding proteins on DNA-polymerase activity of isolated nuclei and nuclear matrix].
It was shown, that tocopherol addition to the incubation medium increased the DNA-polymerase activity of isolated rat liver nuclei. This phenomenon was not discovered in nuclei of vitamin E-depleted rats. The action of tocopherol changed after the extraction of nuclei with triton X-100. In this situation tocopherol decreased DNA-polymerase activity of nuclei. The differences between rates of DNA synthesis was found after simultaneous addition of tocopherol and fraction of tocopherol-binding protein to the nuclei of normal and vitamin E-depleted rats. In this case DNA-polymerase activity of normal nuclei increased, but did not change in nuclei of vitamin E undernourished rats. The difference between them was about 30%. The differences between DNA-polymerase activity of normal and vitamin E-depleted rats after the addition of tocopherol and fraction of tocopherol-binding protein were also found in nuclear matrix preparation. It was supposed that tocopherol was able to take part in functioning of cell nuclei modulating DNA synthesis as well.
Donchenko
G V
GV
Petrova
G V
GV
Kapralov
A A
AA
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology*; D002843:Chromatin / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D015530:Nuclear Matrix / Q000201:enzymology*; D009687:Nuclear Proteins / Q000378:metabolism*; D051381:Rats; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology*
D002352:Carrier Proteins; D002843:Chromatin; D009687:Nuclear Proteins; D014810:Vitamin E; D004259:DNA-Directed DNA Polymerase