title: A molecular scheme based on 23S rRNA gene polymorphisms for rapid identification of Campylobacter and Arcobacter species.
abstract: 23S rRNA gene PCR amplicons from 118 strains representing 15 species of Campylobacter and four species of Arcobacter were consecutively digested with HpaII, CfoI, and HinfI. A reproducible and discriminatory identification scheme based on combined restriction patterns was developed by using reference strains and was applied to the identification of a variety of isolates.
authors.affiliation: Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Hurtado
authors.forename: R J
authors.initials: RJ
authors.lastname: Owen
mesh_terms: D002167:Campylobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S