title: Axon tracts correlate with netrin-1a expression in the zebrafish embryo.
abstract: Netrins are secreted molecules that can attract or repel growth cones from a variety of organisms. In order to clarify the extent and scope of the effects of netrins for guiding growth cones, we have analyzed netrin-1a within the relatively simple and well-characterized nervous system of zebrafish embryos. netrin-1a is expressed in dynamic patterns that suggest that it guides the growth cones of a wide variety of neurons. The spatiotemporal relationship of netrin-1a expression and extending growth cones further suggests that netrins may act to delineate specific pathways and stimulate axonal outgrowth in addition to attracting and repelling growth cones. Furthermore, aberrant outgrowth by commissural growth cones in the spinal cords of floating head mutants, in which netrin-1a expression is altered, is consistent with an in vivo, chemoattractive action of netrin-1a. These data suggest that netrins act on many growth cones and influence their behavior in a variety of ways.
authors.affiliation: Department of Biology, University of Michigan, Ann Arbor 48109-1048, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Lauderdale
authors.forename: N M
authors.initials: NM
authors.lastname: Davis
authors.forename: J Y
authors.initials: JY
authors.lastname: Kuwada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000502:physiology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000196:embryology; D009154:Mutation; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D000075388:Netrin-1; D009434:Neural Pathways / Q000196:embryology; D013116:Spinal Cord / Q000196:embryology / Q000648:ultrastructure; D014018:Tissue Distribution; D025521:Tumor Suppressor Proteins; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins
chemical_list: D018076:DNA, Complementary; D009414:Nerve Growth Factors; D025521:Tumor Suppressor Proteins; D029961:Zebrafish Proteins; C000617355:ntn1a protein, zebrafish; D000075388:Netrin-1