Comparison of a high power diode laser with the Nd:YAG laser using in situ wound strength analysis of healing cutaneous incisions.
OBJECTIVE
The laser-tissue interaction of a high power semiconductor diode laser was compared to the continuous wave neodymium yttrium aluminum garnet (Nd:YAG) laser by evaluating primary wound healing of cutaneous incisions in rats.


METHODS
Full thickness incisions were made in rat skin using a diode laser (805 nm, 10 W, contact mode), an Nd:YAG laser (1,064 nm, 10 W, contact mode), and a stainless steel scalpel blade (control). In situ wound breaking strength measurements were obtained at 7, 14, and 21 days using a specially designed tensiometer. Cross sectional area of non-disrupted wounds was calculated in two groups prior to testing to allow for calculation of tensile strength. Blinded histopathologic analysis was also performed.


RESULTS
Analysis of variance (P < or = 0.05) was used to determine differences in breaking strengths and tensile strengths due to incision method. There was no significant difference in the breaking strengths (group 1) or tensile strengths (groups 2 and 3) of the diode and Nd:YAG laser incisions. As predicted, breaking strengths and tensile strengths of scalpel blade incisions were significantly greater than those of incisions made with laser energy. Histopathologic evaluation revealed that through day 14, the degree of inflammation and collagen production was similar for diode and Nd:YAG laser incisions. Laser incisions had greater inflammation and a lag in fibroblast invasion and collagen production compared with scalpel incisions. By day 21, all incisions were similar in fibroblast population and collagen production, but laser incisions had slightly more inflammation than scalpel incisions.


CONCLUSIONS
In the primary wound healing model described, the tissue effect, cellular response, and development of wound strength were essentially the same for the high power diode laser at 10 W and the Nd:YAG laser at 10 W.
Taylor
D L
DL
Department of Small Animal Medicine and Surgery, Boren Veterinary Medical Teaching Hospital, Stillwater, OK 74078, USA.
Schafer
S A
SA
Nordquist
R
R
Payton
M E
ME
Dickey
D T
DT
Bartels
K E
KE
D000818:Animals; D001696:Biomechanical Phenomena; D003094:Collagen / Q000378:metabolism; D004867:Equipment Design; D005347:Fibroblasts / Q000473:pathology; D007249:Inflammation / Q000473:pathology; D053685:Laser Therapy / Q000295:instrumentation*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000473:pathology; D013718:Tensile Strength; D014945:Wound Healing / Q000502:physiology*
D003094:Collagen