Effects of early surfactant treatment persisting for one week after lung transplantation in rats.
We investigated whether pulmonary surfactant in rat lung transplants recovered during the first week post-transplantation, along with symptoms of the reimplantation response, and whether this recovery was affected by early surfactant treatment. The severity of pulmonary injury was varied by transplanting left lungs with 6-h and 20-h ischemia (n = 12 and 19, respectively). Half of the transplants were treated by instillation of surfactant before reperfusion. Lungs from sham operated, and normal rats (n = 4 and 5, respectively) served as controls. The pulmonary injury severely impaired lung transplant function; 10 of the worst affected animals died. After 1 wk, symptoms of reimplantation response and properties of pulmonary surfactant were assessed. If untreated, the reimplantation response had almost resolved in the 6-h but not in the 20-h ischemia group; pulmonary surfactant, however, continued to be deficient in both ischemia groups (low amounts of surfactant phospholipids and surfactant protein A [SP-A]). Surfactant treatment improved the recovery from injury in the 20-h ischemia group resulting in normal lung function and amounts of surfactant phospholipids. Amounts of SP-A were not improved by surfactant treatment. In conclusion, early surfactant treatment enhances recovery from transplantation injury and is persistently beneficial for pulmonary surfactant in lung transplants.
Erasmus
M E
ME
Department of Cardiopulmonary Surgery, University Hospital Croningen, The Netherlands.
Hofstede
G J
GJ
Petersen
A H
AH
Haagsman
H P
HP
Oetomo
S B
SB
Prop
J
J
D000704:Analysis of Variance; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002417:Cattle; D008055:Lipids / Q000032:analysis / Q000627:therapeutic use*; D008168:Lung / Q000187:drug effects / Q000502:physiology; D016040:Lung Transplantation / Q000379:methods / Q000502:physiology / Q000706:statistics & numerical data; D008297:Male; D010743:Phospholipids; D011184:Postoperative Period; D011663:Pulmonary Surfactants / Q000032:analysis / Q000627:therapeutic use*; D051381:Rats; D011917:Rats, Inbred Lew; D013047:Specific Pathogen-Free Organisms; D018709:Statistics, Nonparametric; D013997:Time Factors; D014185:Transplantation, Isogeneic
D008055:Lipids; D010743:Phospholipids; D011663:Pulmonary Surfactants; C078272:SF-RI 1, bovine surfactant preparation