title: Intraocular pressure and outflow facility are unchanged following acute and chronic intracameral chondroitinase ABC and hyaluronidase in monkeys.
abstract: We determined the effect of chronic and acute loss of glycosaminoglycans from the aqueous outflow pathway on intraocular pressure (IOP) and outflow facility in the subhuman primate eye. For the study of the chronic effects of the GAGases, cynomolgus monkeys received intracameral injections of hyaluronidase (Streptomyces, 5 or 50 units, n=2) or chondroitinase ABC (0.05 or 0.25 units, n=2) biweekly for 8 months (4 months for each dose). IOP was measured at 3, 7, 10 and 14 days after each injection. Outflow facility (2-level constant pressure) was determined at 2, 4, 6, and 8 months. Monkeys were killed 6 days after the last injection. The changes in the distribution of hyaluronic acid and sulfated proteoglycans in the outflow pathway were examined using substrate-specific histochemical techniques. The acute effects of these enzymes on outflow facilities (30 min or 2 hr after enzyme) were determined in another group of animals (n=4 for each time enzyme-1). IOP and outflow facility were unchanged compared to controls (heat inactivated enzyme) at any time in the chronically or acutely treated monkeys. Hyaluronic acid staining was absent in the outflow pathways of eyes treated chronically with hyaluronidase compared with control eyes, while collagen-associated sulfated proteoglycans were decreased but not completely removed by the chronic chondroitinase ABC treatment. Chronic loss of these glycosaminoglycans from the trabecular meshwork does not appear to contribute to the IOP elevation and decrease in outflow facility that accompanies open-angle glaucoma. Most importantly, no increase in outflow facility was found with acute hyaluronidase or chondroitinase treatment.
authors.affiliation: Department of Ophthalmology & Visual Sciences, University of Wisconsin, Madison, WI 53792-3220, USA.
authors.forename: W C
authors.initials: WC
authors.lastname: Hubbard
authors.forename: M
authors.initials: M
authors.lastname: Johnson
authors.forename: H
authors.initials: H
authors.lastname: Gong
authors.forename: B T
authors.initials: BT
authors.lastname: Gabelt
authors.forename: J A
authors.initials: JA
authors.lastname: Peterson
authors.forename: R
authors.initials: R
authors.lastname: Sawhney
authors.forename: T
authors.initials: T
authors.lastname: Freddo
authors.forename: P L
authors.initials: PL
authors.lastname: Kaufman
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects*; D019765:Chondroitin ABC Lyase / Q000494:pharmacology*; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D006651:Histocytochemistry; D006820:Hyaluronic Acid / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology*; D007429:Intraocular Pressure / Q000187:drug effects*; D008252:Macaca fascicularis; D008297:Male
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; D019765:Chondroitin ABC Lyase