title: Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles.
abstract: The virus-like particles (VLPs) produced by the yeast retrotransposon Ty1 are functionally related to retroviral cores. These particles are unusual in that they have variable radif. A paired mass-radius analysis of VLPs by scanning transmission electron microscopy showed that many of these particles form an icosahedral T-number series. Three-dimensional reconstruction to 38-A resolution from cryo-electron micrographs of T = 3 and T = 4 shells revealed that the single structural protein encoded by the TYA gene assembles into spiky shells from trimeric units.
authors.affiliation: Department of Crystallography, Birkbeck College, London, United Kingdom.
authors.forename: K J
authors.initials: KJ
authors.lastname: Palmer
authors.forename: W
authors.initials: W
authors.lastname: Tichelaar
authors.forename: N
authors.initials: N
authors.lastname: Myers
authors.forename: N R
authors.initials: NR
authors.lastname: Burns
authors.forename: S J
authors.initials: SJ
authors.lastname: Butcher
authors.forename: A J
authors.initials: AJ
authors.lastname: Kingsman
authors.forename: S D
authors.initials: SD
authors.lastname: Fuller
authors.forename: H R
authors.initials: HR
authors.lastname: Saibil
mesh_terms: D002213:Capsid / Q000648:ultrastructure; D015925:Cryopreservation; D004271:DNA, Fungal / Q000648:ultrastructure*; D008854:Microscopy, Electron; D018626:Retroelements; D012190:Retroviridae / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014771:Virion
chemical_list: D004271:DNA, Fungal; D018626:Retroelements