Resistance to infection with mouse hepatitis virus (MHV) in the cell clones derived from persistently infected DBT cells with the JHM strain of MHV.
PiD-10 and piD-11 cells that have been established from persistently infected DBT cells with the JHM strain of MHV (JHMV) were resistant to infection with JHMV. There was no significant difference in the amount of adsorbed virus among piD-10, piD-11 and DBT cells. When an expression of mRNA of the MHV receptor in piD-10 and piD-11 cells was analyzed by the RT-PCR method, no significant difference was observed in the intensities of the amplified products among piD-10, piD-11 and DBT cells. Treatment of virus-adsorbed cells with PEG, which induces fusion of the cellular membrane with the viral envelop, causes entry of virus particles into cells. There was no significant difference in the yields of virus between PEG-treated and PEG-untreated cells. The titers of infectious virus internalized into piD-10 and piD-11 cells were the same as those in DBT cells. When piD-10 and piD-11 cells were fused with PEG and infected with JHMV, the yields of infectious virion particles from the fused cells between piD-10 and piD-11 cells were significantly lower than those from the fused cells between DBT and piD-10 or piD-11 cells. The present study showed that resistance of piD-10 and piD-11 cells to JHMV infection is not due to an inhibition of JHMV entry into the cells.
Maeda
A
A
Department of Laboratory Animal Science, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Kon
Y
Y
Watanabe
T
T
Hayashi
M
M
D000818:Animals; D015815:Cell Adhesion Molecules; D002459:Cell Fusion / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D006023:Glycoproteins / Q000096:biosynthesis* / Q000502:physiology; D008561:Membrane Fusion / Q000187:drug effects; D051379:Mice; D006517:Murine hepatitis virus / Q000472:pathogenicity / Q000502:physiology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011991:Receptors, Virus / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014779:Virus Replication
C087701:Ceacam2 protein, mouse; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D012333:RNA, Messenger; D011991:Receptors, Virus; D011092:Polyethylene Glycols