Some biochemical properties and the classification of a range of bacterial haloalkane dehalogenases.
Multivariate analyses and experimental data have been used to evaluate the relationships between eight bacterial hydrolytic haloalkane dehalogenases. The results indicate that seven of the dehalogenases investigated can confidently be placed into two Classes [sensu Slater, Bull and Hardman (1995) Biodegradation 6, 181-189] according to their substrate profiles. The remaining enzyme, isolated from Rhodococcus erythropolis CP9, appears to represent a third Class of haloalkane dehalogenases.
Damborsky
J
J
Laboratory of Environmental Microbiology, Masaryk University, Brno, Czech Republic.
Nyandoroh
M G
MG
Nĕmec
M
M
Holoubek
I
I
Bull
A T
AT
Hardman
D J
DJ
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006867:Hydrolases / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D006867:Hydrolases; C046137:haloalkane dehalogenase