title: Miranda is required for the asymmetric localization of Prospero during mitosis in Drosophila.
abstract: Asymmetric division of Drosophila neuroblasts, sensory organ precursor cells, and cells in the procephalic neurogenic region involves the segregation of Numb and Prospero proteins into one of the two daughter cells. We have isolated a novel gene, miranda, based on the ability of its gene product to interact with the Prospero asymmetric localization domain. miranda expression coincides spatially and temporally with asymmetric cell divisions and asymmetric localization of Prospero. Miranda protein is localized asymmetrically, along with Prospero, to the basal cell membrane during mitosis. Loss of miranda gene function abolishes asymmetric Prospero localization during mitosis. The asymmetric localization of Miranda protein requires inscuteable. Our results suggest that miranda functions downstream of inscuteable and works as an adapter that connects Prospero to the basal cell membrane during asymmetric cell division.
authors.affiliation: Howard Hughes Medical Institute and Department of Physiology, University of California at San Francisco, 94143-0724, USA.
authors.forename: C P
authors.initials: CP
authors.lastname: Shen
authors.forename: L Y
authors.initials: LY
authors.lastname: Jan
authors.forename: Y N
authors.initials: YN
authors.lastname: Jan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000737:chemistry; D002455:Cell Division; D002462:Cell Membrane / Q000502:physiology; D016764:Cell Polarity; D003001:Cloning, Molecular; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D008938:Mitosis; D008954:Models, Biological; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009420:Nervous System / Q000166:cytology / Q000196:embryology; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D012441:Saccharomyces cerevisiae; D014157:Transcription Factors / Q000096:biosynthesis
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C107613:Mira protein, Drosophila; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C073099:pros protein, Drosophila