Oligomeric states and siderophore binding of the ligand-gated FhuA protein that forms channels across Escherichia coli outer membranes.
The channel-forming FhuA protein, which translocates ferrichrome across Escherichia coli outer membranes, binds 1 mol ligand/mol monomer in detergent solution. The protein is homogenous and migrates as a single band with a mobility corresponding to 77 kDa in SDS/PAGE electrophoresis. Analytical ultracentrifugation revealed a monodisperse species (s(20,w) = 3.8 S) with a mass of 77,800 +/- 3200 Da. The properties of ligand binding, determined by two independent methods, revealed one binding site/monomer, but are complicated by a pronounced convexity of the Scatchard plot and a Hill coefficient calculated to be 2.5. This strongly suggests that oligomeric species are present. Cross-linking agents revealed the existence of possibly transient, mostly dimeric and trimeric species. The difference between the FhuA protein in detergent solution and in its native membrane environment may be related to the removal of lateral pressure that exists in situ.
Locher
K P
KP
Biozentrum, University of Basel, Switzerland.
Rosenbusch
J P
JP
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001704:Biopolymers; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015640:Ion Channel Gating; D008024:Ligands; D011485:Protein Binding; D011991:Receptors, Virus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017262:Siderophores / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001704:Biopolymers; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D008024:Ligands; D011991:Receptors, Virus; D017262:Siderophores