NADPH-cytochrome P-450 reductase, cytochrome P-450 2C11 and P-450 1A1, and the aryl hydrocarbon receptor in livers of rats fed methyl-folate-deficient diets.
We investigated three hepatic cytochrome P-450 isozymes and the aryl hydrocarbon (Ah) receptor in rats fed one of the following three diets for 15 months: a diet containing the AIN vitamin mixture (control), the control diet devoid of choline and folate (CFD), or the CFD diet devoid of niacin (CFND). Hepatic tumors developed in all CFD- and CFND-fed rats. Western blot analyses of nontumor hepatic tissue showed that NADPH-cytochrome P-450 reductase (P-450 reductase) increased significantly in the CFD and CFND groups compared with the control group. Hepatic cytochrome P-450 2C11 (CYP2C11) was not detectable in the CFD and CFND groups compared with the control group. Ah receptor and cytochrome P-450 1A1 (CYP1A1) were detected in higher amounts in livers of both deficient groups. CYP1A1 is an enzyme associated with bioactivation of exogenous genotoxins. To our knowledge, this is the first time it has been shown that CYP1A1 and the Ah receptor are induced by dietary deficiencies.
Zhang
J
J
Department of Medicine, University of California, Los Angeles 90095, USA.
Henning
S M
SM
Heber
D
D
Choi
J
J
Wang
Y
Y
Swendseid
M E
ME
Go
V L
VL
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D015153:Blotting, Western; D002796:Choline Deficiency / Q000201:enzymology / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D000072467:Cytochrome P450 Family 2; D004032:Diet; D004435:Eating; D005494:Folic Acid Deficiency / Q000201:enzymology / Q000378:metabolism*; D008099:Liver / Q000737:chemistry* / Q000201:enzymology; D008110:Liver Extracts / Q000737:chemistry; D051379:Mice; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis*; D009525:Niacin / Q000172:deficiency; D011817:Rabbits; D051381:Rats; D011916:Rats, Inbred F344; D018336:Receptors, Aryl Hydrocarbon / Q000032:analysis*; D039181:Steroid 16-alpha-Hydroxylase; D013250:Steroid Hydroxylases / Q000032:analysis; D015430:Weight Gain
D008110:Liver Extracts; D018336:Receptors, Aryl Hydrocarbon; D009525:Niacin; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C450279:CYP2C11 protein, rat; D019363:Cytochrome P-450 CYP1A1; D000072467:Cytochrome P450 Family 2; D039181:Steroid 16-alpha-Hydroxylase; D009251:NADPH-Ferrihemoprotein Reductase