title: Melanization affects susceptibility of Cryptococcus neoformans to heat and cold.
abstract: Cryptococcus neoformans can synthesize melanin from a variety of substrates, including L-dopa (L-3,4-dihydroxyphenylalanine). Growth in minimal medium with L-dopa resulted in progressive accumulation of melanin in stationary phase cells. Melanized and non-melanized yeast cells were exposed to heat (42-47 degrees C) and cold (-20 degrees C), and the percentage of survival determined. Melanized cells were less susceptible to heat than non-melanized cells of the same age. Melanized cells from early stationary phase cultures were less susceptible to cold than non-melanized cells of the same age. However, melanized cells from late stationary phase cultures were more susceptible to cold than non-melanized cells of the same age. There was no statistical difference in susceptibility to heat and cold between melanin-deficient cells grown with and without L-dopa. These results suggest a role for melanin in protection against heat and cold.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Rosas
authors.forename: A
authors.initials: A
authors.lastname: Casadevall
mesh_terms: D003080:Cold Temperature; D003455:Cryptococcus neoformans / Q000254:growth & development / Q000378:metabolism*; D006358:Hot Temperature; D008543:Melanins / Q000096:biosynthesis / Q000502:physiology*
chemical_list: D008543:Melanins