Behavior of carbonate and magnesium ions in the initial crystallites at the early developmental stages of the rat calvaria.
Analysis of the contents of calcium (Ca), magnesium (Mg), phosphate, and carbonate ions in the mineral phase of rat calvaria specimens obtained at different developmental stages indicated that the mineral at the newborn stage contained a negligible amount of carbonate, but a high content of Mg. There was no significant difference in Ca and phosphate (as PO4) contents between the newborn material and that from later stages. A relatively large amount of carbonate was detected in the calvaria from 6-day-old rats, in which only immature crystals were observed, thus indicating the beginning of apatite formation. Furthermore, using laser Raman microprobe analysis we confirmed that the Raman peak at 1120 cm-1 band, indicative of a Mg-CO3 compound, appeared at the 6-day stage. We also observed that the Raman peak at 988 cm-1 found in the samples from the newborn seemed to have shifted to 963-962 cm-1 in the case of those obtained from 6-day-old rats, a shift which suggests the conversion from the non-apatitic to the apatitic form. These results indicate that carbonate ions might facilitate the initiation of crystal development by converting the inhibitory Mg ion into its inactive form (Mg-carbonate compound).
Kakei
M
M
1st Department of Oral Anatomy, Faculty of Chemistry, School of Dentistry, Meikai University, Saitama, Japan.
Nakahara
H
H
Tamura
N
N
Itoh
H
H
Kumegawa
M
M
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D002254:Carbonates / Q000032:analysis*; D003460:Crystallization; D017886:Durapatite / Q000032:analysis; D008274:Magnesium / Q000032:analysis*; D008854:Microscopy, Electron; D010710:Phosphates / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000737:chemistry* / Q000254:growth & development* / Q000648:ultrastructure; D013059:Spectrum Analysis, Raman
D002254:Carbonates; D010710:Phosphates; D017886:Durapatite; D008274:Magnesium