Human immunodeficiency virus-1 entry into purified blood dendritic cells through CC and CXC chemokine coreceptors.
Blood dendritic cells (DC) are susceptible to both macrophage (M) and T-cell line (T) tropic human immunodeficiency virus type 1. The CC chemokines RANTES, macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, eotaxin, and, to a lesser extent, monocyte chemoattractant protein-1 (MCP-1) and MCP-4 blocked entry of M-tropic virus into blood DC. The CXC chemokine, SDF-1, a fusin (CXCR4 chemokine receptor) ligand, and an antifusin antibody inhibited DC entry by T-tropic virus. Purified blood DC contained CCR1, CCR2, CCR3, and CCR5 as well as the CXCR4 chemokine receptor RNA transcripts and high levels of fusin on the cell surface. The coexpression of multiple chemokine receptors offers a molecular mechanism to explain the permissiveness of DC for both M- and T-tropic viruses.
Ayehunie
S
S
Laboratory of Viral Pathogenesis, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA.
Garcia-Zepeda
E A
EA
Hoxie
J A
JA
Horuk
R
R
Kupper
T S
TS
Luster
A D
AD
Ruprecht
R M
RM
D054413:Chemokine CCL11; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000378:metabolism; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism / Q000821:virology*; D005434:Flow Cytometry; D019204:GTP-Binding Proteins / Q000378:metabolism; D015497:HIV-1 / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D066298:In Vitro Techniques; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D019718:Receptors, CXCR4; D018121:Receptors, Cytokine / Q000378:metabolism*; D015728:Receptors, HIV / Q000378:metabolism*; D013601:T-Lymphocytes / Q000821:virology
C515456:CCL11 protein, human; D054413:Chemokine CCL11; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D008565:Membrane Proteins; D019718:Receptors, CXCR4; D018121:Receptors, Cytokine; D015728:Receptors, HIV; D019204:GTP-Binding Proteins