Protein engineering as a powerful tool for the chemical modification of enzymes.
This article discusses the techniques of site-specific mutagenesis and protein engineering and their application in the study of enzyme active sites and the mechanism of enzyme action. Particular emphasis is given to beta-lactamase.
Moreno-Hagelsieb
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Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
Soberon
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D004789:Enzyme Activation; D004798:Enzymes / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering / Q000379:methods*; D013379:Substrate Specificity
D004798:Enzymes