Mannose receptor mediated antigen uptake and presentation in human dendritic cells.
In an immature state, dendritic cells (DC) can capture antigen via at least two mechanisms. First, DC use macropinocytosis for continuous uptake of large amounts of soluble antigens. Second, they express high levels of mannose receptor that can mediate internalization of glycosylated ligands. We found that dendritic cells can present mannosylated antigen 100-1000 fold more efficiently than non-mannosylated antigen. Immunocytochemistry as well as subcellular fractionation demonstrated that the mannose receptor and MHC class II molecules were located in distinct subcellular compartments. These results demonstrate that the mannose receptor endows DC with a high capacity to present glycosylated antigens at very low concentrations.
Engering
A J
AJ
Basel Institute for Immunology, Switzerland.
Cella
M
M
Fluitsma
D M
DM
Hoefsmit
E C
EC
Lanzavecchia
A
A
Pieters
J
J
D017951:Antigen Presentation / Q000502:physiology*; D000941:Antigens / Q000378:metabolism*; D001693:Biological Transport, Active; D002450:Cell Communication; D002451:Cell Compartmentation; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D006031:Glycosylation; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D016253:Microscopy, Immunoelectron; D011956:Receptors, Cell Surface / Q000276:immunology* / Q000378:metabolism; D013347:Subcellular Fractions / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D000949:Histocompatibility Antigens Class II; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface