title: Phylogenetic and narG analysis of a Hyphomicrobium isolate.
abstract: An obligately methylotrophic organism was isolated from a water well that manifested symptoms of biofouling. The isolate was appendaged and utilized methylamine, dimethylamine, trimethylamine, or methanol as the sole carbon and energy source. The isolate exhibited hydroxypyruvate reductase activity, suggesting C1-assimilation via the serine pathway. Fatty acid profiling indicated the predominance of 18:1 cis-fatty acids. The isolate did not grow anaerobically with nitrate as the final electron acceptor. Genomic DNA from the isolate did not hybridize against the narG gene, which encodes the alpha subunit of dissimilatory nitrate reductase in Escherichia coli. The phenotypic data suggested the assignment of the isolate to the genus Hyphomicrobium. The identification was supported by phylogenetic characterization based on 16S rRNA sequence comparisons of the isolate.
authors.affiliation: Department of Microbiology, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210-1292, USA.
authors.forename: L
authors.initials: L
authors.lastname: Tuhela
authors.forename: J B
authors.initials: JB
authors.lastname: Robinson
authors.forename: S
authors.initials: S
authors.lastname: Fishbain
authors.forename: D A
authors.initials: DA
authors.lastname: Stahl
authors.forename: O H
authors.initials: OH
authors.lastname: Tuovinen
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018441:Biofilms; D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D009565:Nitrate Reductases; D050901:Nitrate Reductase