Site-specific DNA-methylase M.BspST5I methylates only one strand of the recognized site.
We recently isolated a site-specific adenine DNA-methylase, M.BspST5I, which methylates only one strand of the recognized site GCATC. The methylated base is indicated by an asterisk.
Matvienko
N N
NN
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Zheleznaya
L A
LA
Zelinskaya
N V
NV
Matvienko
N I
NI
D010582:Bacteriophage lambda / Q000737:chemistry / Q000235:genetics; D019175:DNA Methylation; D004274:DNA, Recombinant / Q000235:genetics / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D017422:Sequence Analysis, DNA; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism*
D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D015247:Deoxyribonuclease HindIII