title: A human monoclonal antibody which inhibits the coaggregation activity of Porphyromonas gingivalis.
abstract: A B-cell line producing a human monoclonal antibody (HuMAb) against a recombinant 40-kDa outer membrane protein (OMP) of Porphyromonas gingivalis was constructed by in vivo immunization of a severe combined immunodeficiency C.B.-17/Icr mouse, which had been injected with human peripheral blood lymphocytes, with recombinant 40-kDa OMP and subsequent Epstein-Barr virus immortalization of B cells isolated from the spleen of the mouse. This HuMAb inhibited coaggregation between P. gingivalis vesicles and Actinomyces naeslundii cells.
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan. yabiko@mascat.nihon-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Abiko
authors.forename: N
authors.initials: N
authors.lastname: Ogura
authors.forename: U
authors.initials: U
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Yanagi
authors.forename: H
authors.initials: H
authors.lastname: Takiguchi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D016966:Porphyromonas gingivalis / Q000276:immunology*; D011994:Recombinant Proteins
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins