Upregulation of the elongation factor-1alpha gene by p53 in association with death of an erythroleukemic cell line.
Genes upregulated by p53 were screened using an erythroleukemic cell line (1-2-3) that expresses only the temperature-sensitive p53 by the mRNA differential display method. One of the upregulated genes was identified as the elongation factor-1alpha (EF-1alpha) gene, an essential component of the eukaryotic translation apparatus. Three p53-responsive elements were found in the mouse EF-1alpha gene and in the corresponding human, rat, and frog genes. These elements conferred the capacity for induction by p53. EF-1alpha is also a microtubule-severing protein. Upon the temperature-shift, the cells developed the morphology and the localization of alpha-tubulin similar to those of the cells treated with vincristine, a drug that affects microtubules. The microtubule-severing associated with upregulation of EF-1alpha by p53 may be a cause of the cell death.
Kato
M V
MV
Molecular Oncology Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Sato
H
H
Nagayoshi
M
M
Ikawa
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001483:Base Sequence; D016923:Cell Death / Q000235:genetics / Q000502:physiology; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D008969:Molecular Sequence Data; D017239:Paclitaxel / Q000494:pharmacology; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics* / Q000378:metabolism; D011898:Ranidae; D051381:Rats; D012261:Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D015854:Up-Regulation; D014750:Vincristine / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012261:Ribonucleoproteins; D016159:Tumor Suppressor Protein p53; D014750:Vincristine; D017239:Paclitaxel