[Radioimmunologic methods for determining cortisol binding globulin-CBG in human serum].
The authors described double antibodies radioimmunoassay of human cortisol binding globulin serum level in support of previous isolated and purified trancortin and anti-transcortin antibodies produced by rabbit immunization. Antigen was iodinated by chloraminet method and purification on Ultragel AcA 54 column. Standard curve had specific Bx/Box100%. Using described method investigation of transcortin globulin serum level is precise and repetitive.
Głyda
A
A
Instytutu Ginekologii i Połoznictwa Akademii Medycznej w Poznaniu.
Kozak
W
W
Waśko
R
R
Junik
R
R
Krawczyk
J
J
D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D011262:Pregnancy Trimester, Second / Q000097:blood*; D011263:Pregnancy Trimester, Third / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002352:Carrier Proteins; C027091:cortisol binding globulin