Ligand-binding specificity of human fibroblast growth factor receptor-3 IIIc.
Earlier studies indicated that human fibroblast growth factor receptor (FGFR)-3 IIIc was activated equally well by both FGF-1 and FGF-2. In contrast, murine FGFR-3 IIIc was preferentially activated by FGF-1. To address this issue, we determined the ligand-binding specificity of human FGFR-3 IIIc in comparison with human FGFR-1 IIIc. By equilibrium binding human FGFR-3 IIIc preferentially bound FGF-1 with high affinity, whereas FGFR-1 IIIc bound both FGF-1 and -2 with high affinity. By competition binding using FGF-1, -2, -4, or -6, FGF-1 competed more efficiently than the other FGFs. These results suggest that like the murine FGFR-3 III, FGF-1 is a preferred ligand for human FGFR-3 IIIc.
Lin
H Y
HY
Graduate Program in Molecular Biology and Biochemistry, State University of New York at Stony Brook, 11794-5222, USA.
Kaplow
J
J
Jaye
M
M
Hayman
M J
MJ
D000818:Animals; D001667:Binding, Competitive; D015153:Blotting, Western; D002460:Cell Line; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D051519:Fibroblast Growth Factor 4; D051522:Fibroblast Growth Factor 6; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051498:Receptor, Fibroblast Growth Factor, Type 3; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism*
C496445:FGF4 protein, human; C496452:FGF6 protein, human; C496447:Fgf4 protein, rat; C486116:Fgf6 protein, rat; D051519:Fibroblast Growth Factor 4; D051522:Fibroblast Growth Factor 6; D008024:Ligands; D011518:Proto-Oncogene Proteins; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors; C496348:FGFR1 protein, human; C496365:FGFR3 protein, human; C496350:Fgfr1 protein, rat; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D051498:Receptor, Fibroblast Growth Factor, Type 3