Production of chitinase from shellfish waste by Pseudomonas aeruginosa K-187.
The production of chitinolytic enzyme by Pseudomonas aeruginosa K-187, using shrimp and crab shell powder (SCSP) as the carbon source, was studied. It was observed that chemically treated SCSP induced a significant increase of enzyme production, as compared with untreated SCSP. Spent HCl and NaOH from the chitin production industry was used to process SCSP. Various strategies of SCSP processing are examined and compared in terms of chitinolytic enzyme production. A three-and-one-half-fold increase of enzyme production (0.68 U/ml to 2.4 U/ml) was attained using HCl/NaOH treated SCSP. The microorganism (K-187) was isolated from soil in Taiwan and has been characterized and reported in a previous paper.
Wang
S L
SL
Department of Food Engineering, Da-Yeh Institute of Technology, Chang-Hwa, Taiwan, Republic of China.
Chiou
S H
SH
Chang
W T
WT
D000117:Acetylglucosamine / Q000494:pharmacology; D000645:Ammonium Sulfate / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002244:Carbon / Q000378:metabolism*; D002686:Chitin / Q000031:analogs & derivatives / Q000302:isolation & purification* / Q000494:pharmacology; D002688:Chitinases / Q000096:biosynthesis*; D048271:Chitosan; D004790:Enzyme Induction; D005399:Fishes; D018698:Glutamic Acid / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D011208:Powders; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D012758:Shellfish
D001426:Bacterial Proteins; D007220:Industrial Waste; D009566:Nitrates; D011208:Powders; D002686:Chitin; D018698:Glutamic Acid; D002244:Carbon; C031618:sodium nitrate; D048271:Chitosan; D002688:Chitinases; D009584:Nitrogen; D000645:Ammonium Sulfate; D000117:Acetylglucosamine