Alcohol dehydrogenase-I from horse liver stimulates immunoglobulin production by human hybridoma and human peripheral blood lymphocytes.
Immunoglobulin production stimulating activity of alcohol dehydrogenase [EC 1.1.1.1] was assessed. Alcohol dehydrogenase-I (ADH-I) derived from horse liver stimulated IgM production by human-human hybridoma, HB4C5 cells producing human lung cancer specific monoclonal IgM. IgM production of HB4C5 cells was enhanced more than 6 fold by the addition of ADH-I at 400 microg/ml under serum-free condition. However, yeast derived ADHs, such as ADH-II and -III were ineffective to accelerate immunoglobulin production of the hybridoma line. These results imply that the immunoglobulin production stimulating effect of ADH-I is irrelevant to its enzymatic function, and defined as a novel feature of ADH-I. This enzyme also stimulated IgM and IgG production by human peripheral blood lymphocytes 2.9 fold and 1.4 fold, respectively. This fact suggests that ADH-I stimulates immunoglobulin production not only by specific hybridoma cell line, but also by non-specific immunoglobulin producers.
Sugahara
T
T
College of Agriculture, Ehime University, Matsuyama, Japan.
Furutani
H
H
Sasaki
T
T
D000426:Alcohol Dehydrogenase / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D016895:Culture Media, Serum-Free; D004305:Dose-Response Relationship, Drug; D006736:Horses; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism*; D007074:Immunoglobulin G / Q000187:drug effects / Q000378:metabolism; D007075:Immunoglobulin M / Q000187:drug effects / Q000378:metabolism; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000187:drug effects*; D007527:Isoenzymes / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008214:Lymphocytes / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*; D013997:Time Factors
D016895:Culture Media, Serum-Free; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007527:Isoenzymes; D011506:Proteins; D000426:Alcohol Dehydrogenase