Influence of LEPK on biochemical activity and morphology in situ of liver Golgi apparatus from control and streptozotocin-diabetic rats.
This paper presents yields of Golgi-rich membrane isolation, the activity of galactosyltransferase (GalT), the marker enzyme of Golgi apparatus as well as the morphology of the organelle from the livers in situ, in two groups of rats. One group consisted of control rats injected twice intraperitoneally with LEPK. Second group consisted of rats injected with LEPK and additionally after 24hrs given streptozotocin (SZ) to induce experimental diabetes. The results were compared with our previous investigations in control and diabetic rats. In the latter the activity of GalT was diminished, therefore diminishing glycosylation ability, and destructing Golgi apparatus morphology. This experiment shows that two-fold injection of LEPK prior to SZ does not prevent from changes in such biochemical parameters as free blood glucose level, yield of liver Golgi membranes isolation or total activity of GalT. For the first time in c. 30% of investigated rats the inactive enzyme of Golgi apparatus was found in the rats treated with LEPK+SZ. Morphological investigations of liver Golgi apparatus in rats treated with LEPK show slightly increased secretory activity with similar to untreated control rats morphological structure of this organelle. In the rats treated with LEPK and SZ the same morphological changes as in diabetic liver were found, however, such dramatic alterations as in SZ-diabetic rats were never found, irrespective of active or inactive GalT.
Kordowiak
A M
AM
Institute of Molecular Biology, Jagiellonian University, Kraków.
Polański
M
M
Dabroś
W
W
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005700:Galactosyltransferases / Q000378:metabolism; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011058:Pollen / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D010936:Plant Extracts; D005700:Galactosyltransferases