title: [The presence of Human Papillomavirus (HPV) in microinvasive in situ spinocellular carcinoma of the oral cavity. Preliminary report].
abstract: BACKGROUND
  The authors examined 15 cases of in situ and early oral SCC to detect the presence of HPV genoma.
  
  
  METHODS
  They proceeded to in situ hybridization on paraffined specimens utilizing DNA-biotinylated fragments in commercial kit (Vyra Type in situ, Life Technologies, Gaithersburg, MD, USA).
  
  
  RESULTS
  HPV-DNA was detected in 9 cases: 6/11, 16/18, 31/33/35 serotypes were detected. The distribution of viral serotypes by sites showed a prevalence of 16/18 serotype in tongue and floor of the mouth carcinoma, a prevalence of 6/11 serotype in gingiva, hard palate and buccal mucosa carcinoma, and 31/33/35 in commissura.
  
  
  CONCLUSIONS
  The results suggest an important role of HPV in oral carcinogenesis, but further investigations are necessary to confirm these data on larger groups of patients to obtain a higher significance of the results.
authors.affiliation: Facoltà di Medicina e Chirurgia, Istituto di Discipline Odontostomatologiche, Università degli Studi di Napoli Federico II-Napoli.
authors.forename: M D
authors.initials: MD
authors.lastname: Mignogna
authors.forename: R
authors.initials: R
authors.lastname: Duraccio
authors.forename: R
authors.initials: R
authors.lastname: Carbone
authors.forename: R E
authors.initials: RE
authors.lastname: Mignogna
authors.forename: L
authors.initials: L
authors.lastname: Lo Muzio
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D002277:Carcinoma / Q000175:diagnosis / Q000473:pathology / Q000601:surgery / Q000821:virology*; D002278:Carcinoma in Situ / Q000473:pathology / Q000601:surgery / Q000821:virology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000601:surgery / Q000821:virology*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000601:surgery / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery