title: Characterization of Ca2+ transport activity associated with a non-mitochondrial calcium pool in the rodent malaria parasite P. chabaudi.
abstract: Non-mitochondrial calcium deposits were investigated in the intraerythrocytic malaria parasite Plasmodium chabaudi at the trophozoite stage by means of arsenazo III in the presence of ATP and the mitochondrial poisons, antimycin and oligomycin. Addition of vanadate and 2,5-di-(t-butyl)-1,4-hydroquinone (BHQ), both known to interact with SERCA pump, induced calcium release by permeabilized parasites when the medium free calcium concentration was kept at 3.5 microM. The tumor promoter thapsigargin also caused elevation of the free calcium concentration in permeabilized parasites. Our results support the view that P. chabaudi sequesters calcium in an exchangeable form and maintains its calcium homeostasis by way of an endoplasmic reticulum Ca2+ pump.
authors.affiliation: Departamento de Fisiologia, Universidade de São Paulo, Brasil.
authors.forename: A P
authors.initials: AP
authors.lastname: Passos
authors.forename: C R
authors.initials: CR
authors.lastname: Garcia
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006873:Hydroquinones / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D016788:Plasmodium chabaudi / Q000378:metabolism* / Q000648:ultrastructure; D012377:Rodentia / Q000469:parasitology; D019284:Thapsigargin / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D006873:Hydroquinones; D014638:Vanadates; D019284:Thapsigargin; D002118:Calcium