Effects of S-adenosyl-L-methionine on platelet thromboxane and vascular prostacyclin.
We therefore designed the present study to evaluate the effect of S-adenosyl-L-methionine (SAMe) on the synthesis of platelet thromboxane and vascular prostacyclin. The experimental materials were human blood and aortic rings from untreated Wistar rats; and platelets and aortic rings from Wistar rats treated for 7 days with SAMe at 5 or 10 mg/kg/day s.c. The administration of 10 mg/Kg/day of SAMe to rats significantly increased vascular production of 6-keto-PGF1alpha. In vitro vascular production of 6-keto-PGF1alpha increased in a concentration-dependent manner when SAMe was incubated in the range of 10(-7) to 10(-4) M. The greatest increase was 167 +/- 15%, obtained in samples incubated with 5 x 10(-5) M SAMe. In aortic rings, lipid peroxidase production was inhibited in a concentration-dependent manner in the SAMe range of 10(-7) to 10(-5) M. Maximum inhibition (75.3 +/- 6.2%) was obtained with SAMe at 1.5 x 10(-5) M. Vascular 6-keto-PGF1alpha production showed a significant inverse linear correlation with vascular lipid peroxide production (Y = -0.04x + 18.1, r = 0.7309, P < 0.0001).
De La Cruz
J P
JP
Department of Pharmacology and Therapeutics, School of Medicine, University of Málaga, Spain.
González-Correa
J A
JA
Martín-Aurioles
E
E
Ortiz
P
P
Sánchez de la Cuesta
F
F
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D001011:Aorta / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000096:biosynthesis; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013929:Thromboxane B2 / Q000096:biosynthesis*
D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D012436:S-Adenosylmethionine; D011464:Epoprostenol