Differential effects of dimethyl sulfoxide on nicotinic acetylcholine receptors from mouse muscle and Torpedo electrocytes.
The present study examined the effects of 0.1% dimethyl sulfoxide (DMSO) on nicotinic acetylcholine receptors (nAChR) from mouse muscle and Torpedo californica electrocytes. Receptors were expressed in Xenopus laevis oocytes and studied with voltage-clamp. When applied simultaneously with acetylcholine, DMSO did not inhibit current amplitude of either receptor. Preincubation with DMSO for 1 min reduced current amplitude by approximately 50% from oocytes expressing electrocyte receptor. Preincubation did not affect the muscle receptor. With electric organ membranes, 0.1% DMSO did not block either [alpha-(125)I]bungarotoxin binding to the nAChR agonist site or [3H]phencyclidine binding to its high affinity site on resting or desensitized receptor. These data suggest that DMSO might be affecting the electrocyte receptor through a second messenger system.
Eaton
M J
MJ
Department of Biochemistry and Nutrition, Center for Molecular and Behavioral Neuroscience, Universidad Central del Caribe, Bayamón, Puerto Rico. eaton@coqui.net
Pagán
O R
OR
Hann
R M
RM
Eterović
V A
VA
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002038:Bungarotoxins / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D007457:Iodine Radioisotopes; D015640:Ion Channel Gating / Q000187:drug effects*; D051379:Mice; D009865:Oocytes / Q000737:chemistry / Q000502:physiology; D018408:Patch-Clamp Techniques; D010622:Phencyclidine / Q000494:pharmacology; D011869:Radioligand Assay; D011978:Receptors, Nicotinic / Q000502:physiology*; D014101:Torpedo; D014316:Tritium; D014982:Xenopus laevis
D000893:Anti-Inflammatory Agents; D002038:Bungarotoxins; D018691:Excitatory Amino Acid Antagonists; D007457:Iodine Radioisotopes; D011978:Receptors, Nicotinic; D014316:Tritium; D010622:Phencyclidine; D004121:Dimethyl Sulfoxide