Leptin inhibits glycogen synthesis in the isolated soleus muscle of obese (ob/ob) mice.
The ob gene product, leptin, causes significant and dose-dependent inhibition of basal and insulin-stimulated glycogen synthesis in isolated soleus muscle from ob/ob mice, and a smaller, non-significant inhibition in muscle from wild-type mice. Leptin had no inhibitory effect on glycogen synthesis in soleus muscle from the diabetic (db/db) mice, which lack the functional leptin receptor. The full-length leptin receptor (Ob-Rb), is expressed in soleus muscle of both ob/ob and wild-type mice, however with no detectable differences in expression level. These results suggest that hyperleptinaemia may attenuate insulin action on glucose storage in skeletal muscle.
Liu
Y L
YL
Clore Laboratory, University of Buckingham, UK.
Emilsson
V
V
Cawthorne
M A
MA
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis* / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D020738:Leptin; D051379:Mice; D008811:Mice, Inbred DBA; D008817:Mice, Mutant Strains; D008820:Mice, Obese; D018482:Muscle, Skeletal / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D011994:Recombinant Proteins / Q000494:pharmacology
D002352:Carrier Proteins; D007328:Insulin; D020738:Leptin; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D011994:Recombinant Proteins; C515447:leptin receptor, mouse; D006003:Glycogen; D005947:Glucose