Suppressed formation of bone marrow adherent layers derived from acute myeloid leukemia patients after in vitro exposure to interleukin-4.
Long-term bone marrow cultures from ten acute myeloid leukemia (AML) patients exposed to recombinant human (rhu) interleukin (IL) 4 from culture initiation failed to develop adherent layers at 5 weeks as compared to controls. The adherent layers from two of our patients expressed IL-1 beta transcripts constitutively, and all produced IL-6 and leukemia inhibitory factor (LIF) proteins. Our results demonstrate that rhuIL-4 markedly inhibited AML-derived adherent layer formation in a time- and dose-dependent manner, and this effect was not mediated through enhanced apoptosis and did not correlate with IL-1 beta, IL-6 or LIF production.
Wetzler
M
M
Department of Hematologic Oncology, Roswell Park Cancer Institute, State University of New York at Buffalo, NY 14263, USA.
Kurzrock
R
R
Estrov
Z
Z
Barone
S
S
Estey
E
E
Talpaz
M
M
D000208:Acute Disease; D017209:Apoptosis; D001853:Bone Marrow / Q000473:pathology*; D002448:Cell Adhesion; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D015847:Interleukin-4