title: Evaluation of a new protocol for peripheral blood stem cell collection with the Fresenius AS 104 cell separator.
abstract: In this report we analyzed sixty leukapheresis procedures on 35 patients with a new protocol for the Fresenius AS 104. Yields and efficiencies for MNC, CD 34+ cells, and CFU-GM indicate that the new protocol is able to collect large quantities of hemopoietic progenitors. Procedures were performed processing 8.69 +/- 2.8 liters of whole blood per apheresis and modifying 3 parameters: spillover-volume 7 ml, buffy-coat volume 11.5 ml, centrifuge speed 1,500 rpm; blood flow rate was 50 ml/min and the anticoagulant ratio was 1:12. No side effects were observed during apheresis procedures except for transient paresthesia episodes promptly resolved with the administration of calcium gluconate. Yields show a high capacity of the new program to collect on average MNC 17.28 +/- 10.85 x 10(9), CD 34+ 471 +/- 553.5 x 10(6) and CFU-GM 1278.7 +/- 1346.3 x 10(4) per procedure. Separator collection efficiency on average was 49.91 +/- 23.28% for MNC, 55.1 +/- 35.66% for CFU-GM, and 62.97 +/- 23.09% for CD 34+ cells. Particularly interesting are results for MNC yields and CD 34+ efficiency; these results make the new program advantageous or similar to the most progressive blood cell separators and capable to collect a sufficient number of progenitor cells for a graft with a mean of 1.80 +/- 0.98 procedures per patient.
authors.affiliation: Centro Ricerche per la Manipolazione dei Costituenti Ematici, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Menichella
authors.forename: M
authors.initials: M
authors.lastname: Lai
authors.forename: L
authors.initials: L
authors.lastname: Pierelli
authors.forename: R
authors.initials: R
authors.lastname: Serafini
authors.forename: M
authors.initials: M
authors.lastname: Vittori
authors.forename: M
authors.initials: M
authors.lastname: Ciarli
authors.forename: M L
authors.initials: ML
authors.lastname: Foddai
authors.forename: G
authors.initials: G
authors.lastname: Puglia
authors.forename: H
authors.initials: H
authors.lastname: Mitschulat
authors.forename: G
authors.initials: G
authors.lastname: Scambia
authors.forename: G
authors.initials: G
authors.lastname: Leone
authors.forename: B
authors.initials: B
authors.lastname: Bizzi
mesh_terms: D000293:Adolescent; D000328:Adult; D001781:Blood Component Removal / Q000295:instrumentation*; D002648:Child; D002675:Child, Preschool; D006412:Hematopoietic Stem Cells; D006801:Humans; D008875:Middle Aged