RNA-mediated regulation of Receptor-Ck gene in human platelets.
The study addressed to understand the regulation of Receptor-'Ck' gene at the translational level revealed that exogenous cholesterol has the inherent capacity to regulate the endogenous synthesis of Receptor-'Ck' by initiating intracellular targeting of the Receptor-'Ck' to the mRNP pool within human platelets and this effect could be reversed when the platelets were incubated with cholesterol coupled with either dB cAMP or dB cGMP. Based upon these observations, we propose that Receptor-'Ck' initiated signalling, which involves second messengers like PA, cAMP and cGMP, may be responsible for the autoregulation of Receptor-'Ck' gene expression at the translational level.
Singh
J
J
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Kaul
D
D
D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002784:Cholesterol / Q000008:administration & dosage / Q000494:pharmacology; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D004359:Drug Therapy, Combination; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012313:RNA / Q000494:pharmacology*; D020794:Receptor Protein-Tyrosine Kinases / Q000097:blood* / Q000187:drug effects / Q000235:genetics*; D018110:Receptors, Lipoprotein / Q000097:blood* / Q000187:drug effects / Q000235:genetics*; D012261:Ribonucleoproteins / Q000032:analysis
D018110:Receptors, Lipoprotein; D012261:Ribonucleoproteins; D003995:Dibutyryl Cyclic GMP; D012313:RNA; D003994:Bucladesine; D002784:Cholesterol; D020794:Receptor Protein-Tyrosine Kinases