title: [Simplified analytical method of 99mTc-labeled phosphonate].
abstract: A simplified and rapid analytical method of 99mTc-labeled phosphonates was tested using a mini-column based on an anion-exchange type of cartridge. Free 99mTcO4- in the prepared solutions of 99mTc-labeled phosphonate was eluted from the column by a neutral phosphate buffer solution. Partly components of the 99mTc-labeled phosphonates was eluted from the column by a 100 mM sodium phosphonate solution, while the residual components were not eluted from the mini-column. In addition, for analysis of 99mTc-labeling rate in 99mTc-MDP solution, this method requires much less time than thin layer chromatography (TLC). Therefore, the method is more suitable for analysis of 99mTc-labeled phosphonates than TLC now in use, particularly rapid analysis for 99mTc-labeling rate of the compounds and the stability.
authors.affiliation: Department of Oral and Maxillofacial Radiology, Kanagawa Dental College.
authors.forename: Y M
authors.initials: YM
authors.lastname: Okamoto
authors.forename: H
authors.initials: H
authors.lastname: Wakao
mesh_terms: D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D002855:Chromatography, Thin Layer; D004355:Drug Stability; D019275:Radiopharmaceuticals / Q000032:analysis*; D013670:Sodium Pertechnetate Tc 99m / Q000032:analysis*; D013669:Technetium Tc 99m Medronate / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D019275:Radiopharmaceuticals; C024226:technetium Tc 99m hydroxymethylene diphosphonate; D013670:Sodium Pertechnetate Tc 99m; D013669:Technetium Tc 99m Medronate