title: Structural and functional studies of hemoglobin Moabit (alpha 86(F7) Leu-->Arg.
abstract: An abnormal hemoglobin fraction was detected on high performance liquid chromatography profile performed for the measurement of glycated hemoglobin in a 55-year-old caucasian patient. The structural and functional studies were performed by standard techniques. Separation of hemoglobins by alkaline electrophoresis and by IEF revealed a slightly more rapid fraction than does Hb S. By acid electrophoresis, no abnormal Hb fraction could be observed. Separation of globin chains by electrophoresis demonstrated an alpha-chain variant and by chromatography, a fraction which eluted between beta and gamma globin chains. Tryptic digests and amino acid analysis have demonstrated a previously described substitution of Leu-->Arg alpha 86(F7).
authors.affiliation: Department of Clinical Chemistry, Hôpital Erasme, Bruxelles, Belgique.
authors.forename: B
authors.initials: B
authors.lastname: Gulbis
authors.forename: L
authors.initials: L
authors.lastname: Tshilolo
authors.forename: H
authors.initials: H
authors.lastname: Wajcman
authors.forename: J
authors.initials: J
authors.lastname: Riou
authors.forename: F
authors.initials: F
authors.lastname: Galactéros
authors.forename: D
authors.initials: D
authors.lastname: Promé
authors.forename: J
authors.initials: J
authors.lastname: Kister
authors.forename: I
authors.initials: I
authors.lastname: Papassotiriou
authors.forename: Y
authors.initials: Y
authors.lastname: Van Laethem
authors.forename: F
authors.initials: F
authors.lastname: Vertongen
mesh_terms: D001797:Blood Protein Electrophoresis; D002851:Chromatography, High Pressure Liquid; D005914:Globins / Q000737:chemistry / Q000235:genetics; D006442:Glycated Hemoglobin / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D006455:Hemoglobins, Abnormal / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007525:Isoelectric Focusing; D007930:Leucine / Q000235:genetics* / Q000502:physiology; D008103:Liver Cirrhosis / Q000097:blood; D008297:Male; D008875:Middle Aged; D010100:Oxygen / Q000378:metabolism; D017354:Point Mutation / Q000235:genetics
chemical_list: D005914:Globins; D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; D007930:Leucine; D010100:Oxygen