Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles.
The virus-like particles (VLPs) produced by the yeast retrotransposon Ty1 are functionally related to retroviral cores. These particles are unusual in that they have variable radif. A paired mass-radius analysis of VLPs by scanning transmission electron microscopy showed that many of these particles form an icosahedral T-number series. Three-dimensional reconstruction to 38-A resolution from cryo-electron micrographs of T = 3 and T = 4 shells revealed that the single structural protein encoded by the TYA gene assembles into spiky shells from trimeric units.
Palmer
K J
KJ
Department of Crystallography, Birkbeck College, London, United Kingdom.
Tichelaar
W
W
Myers
N
N
Burns
N R
NR
Butcher
S J
SJ
Kingsman
A J
AJ
Fuller
S D
SD
Saibil
H R
HR
D002213:Capsid / Q000648:ultrastructure; D015925:Cryopreservation; D004271:DNA, Fungal / Q000648:ultrastructure*; D008854:Microscopy, Electron; D018626:Retroelements; D012190:Retroviridae / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014771:Virion
D004271:DNA, Fungal; D018626:Retroelements