title: Mature pulmonary lobar transplants grow in an immature environment.
abstract: OBJECTIVE
  Mature lobar transplantation will increase the pediatric donor organ pool, but it remains unknown whether such grafts will grow in a developing recipient and provide adequate long-term support. We hypothesized that a mature pulmonary lobar allograft implanted in an immature recipient would grow.
  
  
  METHODS
  We investigated our hypothesis in a porcine orthotopic left lung transplant model using animals matched by the major histocompatibility complex to minimize the effects of chronic rejection. Twenty-three immature animals (< 12 weeks of age and < 10 kg total body weight) received either sham left thoracotomy (SH control, n = 4), left upper lobectomy to study compensatory growth (UL control, n = 4), age-matched immature whole left lung transplants (IWL TXP, n = 6), mature (donor > 1 yr in age and > 40 kg in total body weight) left lower lobe transplants (MLL TXP, n = 5), or mature left upper lobe transplants (MUL TXP, n = 4). Twelve weeks after implantation, functional residual capacity of the left lung was measured and arterial blood gas samples were obtained after the native right lung had been excluded. The graft was excised and weighed, and samples for microscopy and wet/dry ratios were collected.
  
  
  RESULTS
  Initial and final graft weights were as follows: IWL TXP group (34.6 +/- 1.5 and 107.8 +/- 5.9 gm, p < 0.0001), MLL TXP group (72.4 +/- 6.8 and 111.4 +/- 8.7, p < 0.001), and MUL TXP group (32.8 +/- 1.3 and 92.8 +/- 7.1 gm, respectively, p < 0.004). No significant differences between groups were demonstrated when functional residual capacity, wet/dry ratios, or oxygenation were compared. Immunohistochemical staining for the nuclear antigen Ki-67 demonstrated dividing pneumocytes.
  
  
  CONCLUSIONS
  We conclude that a mature lobar graft implanted into an immature recipient grows by pneumocyte division in this model. Mature lobar transplants can be expected to grow and provide adequate long-term function in developing recipients.
authors.affiliation: Department of Surgery, University of Virginia Health Sciences Center, Charlottesville, USA.
authors.forename: O A
authors.initials: OA
authors.lastname: Binns
authors.forename: N F
authors.initials: NF
authors.lastname: DeLima
authors.forename: S A
authors.initials: SA
authors.lastname: Buchanan
authors.forename: M B
authors.initials: MB
authors.lastname: Lopes
authors.forename: J T
authors.initials: JT
authors.lastname: Cope
authors.forename: C A
authors.initials: CA
authors.lastname: Marek
authors.forename: R C
authors.initials: RC
authors.lastname: King
authors.forename: V E
authors.initials: VE
authors.lastname: Laubach
authors.forename: C G
authors.initials: CG
authors.lastname: Tribble
authors.forename: I L
authors.initials: IL
authors.lastname: Kron
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D005652:Functional Residual Capacity; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008168:Lung / Q000254:growth & development* / Q000378:metabolism; D016040:Lung Transplantation / Q000502:physiology*; D009929:Organ Size; D011013:Pneumonectomy; D013552:Swine; D014184:Transplantation, Homologous
chemical_list: D019394:Ki-67 Antigen