Challenge of BALB/c mice with respiratory syncytial virus does not enhance the Th2 pathway induced after immunization with a recombinant G fusion protein, BBG2NA, in aluminum hydroxide.
The polypeptide of aa 130-230 of the G protein (G2Na) of respiratory syncytial virus (RSV) was fused to BB, the albumin-binding region of streptococcal G protein, producing BBG2Na, which induced protective immune responses in rodent models. Evaluation of the immune response in mice immunized with BBG2Na in the adjuvant alhydrogel revealed high amounts of interleukin (IL)-5 and some IL-4 in splenocytes restimulated in vitro. This is compatible with a Th2 response. The activation of the Th2 pathway in such mice was further supported by the detection of IL-5 and G2Na-specific IgE in vivo. Of interest, in contrast to immunization with formalin-inactivated RSV, immunization of mice with BBG2Na followed by intranasal RSV challenge did not lead to increased production of IL-5- or G2Na-specific IgE. However, IgG1- and IgG2a-specific antibodies were boosted. These results demonstrate that the Th2 pathway is not enhanced by RSV challenge in BBG2Na-immunized mice.
Corvaïa
N
N
Centre d'Immunologie Pierre Fabre, Saint-Julien en Genevois, France.
Tournier
P
P
Nguyen
T N
TN
Haeuw
J F
JF
Power
U F
UF
Binz
H
H
Andréoni
C
C
D000276:Adjuvants, Immunologic; D000536:Aluminum Hydroxide / Q000008:administration & dosage*; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology; D002478:Cells, Cultured; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D015707:HN Protein; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D018984:Epitopes, T-Lymphocyte; D015707:HN Protein; C034284:IgG Fc-binding protein, Streptococcus; D015848:Interleukin-5; D010455:Peptides; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C116204:attachment protein G; D007073:Immunoglobulin E; D000536:Aluminum Hydroxide