Human immunodeficiency virus type 1 DNA and RNA load in brains of demented and nondemented patients with acquired immunodeficiency syndrome.
The relationship between dementia and human immunodeficiency virus type 1 (HIV-1) cerebral load is not clearly understood. We used immunohistochemistry and competitive polymerase chain reaction to evaluate the density ofgp 41 immunostained cells and the amount of HIV-1 DNA and RNA in the midfrontal gyrus of 21 HIV-1 infected patients, nine of whom were demented. The amounts of HIV-1 DNA and RNA, and the density of gp 41-positive cells were significantly linked. In this small series of cases, (1) although as a mean, there was a larger viral load in demented patients than in nondemented, this did not reach the significance level (2) discrepancies appeared in the population under study, some demented patients having low viral loads.
Lazarini
F
F
Laboratoire de Neuropathologie Raymond Escourolle, INSERM U 360, Association Claude Bernard, Paris, France.
Seilhean
D
D
Rosenblum
O
O
Suarez
S
S
Conquy
L
L
Uchihara
T
T
Sazdovitch
V
V
Mokhtari
K
K
Maisonobe
T
T
Boussin
F
F
Katlama
C
C
Bricaire
F
F
Duyckaerts
C
C
Hauw
J J
JJ
D015526:AIDS Dementia Complex / Q000235:genetics / Q000473:pathology / Q000821:virology*; D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000473:pathology / Q000821:virology*; D000328:Adult; D000368:Aged; D001921:Brain / Q000821:virology*; D015331:Cohort Studies; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D019562:Viral Load
D004279:DNA, Viral; D012367:RNA, Viral