An approach combining rapid cDNA amplification and chemical synthesis for the identification of novel, cathelicidin-derived, antimicrobial peptides.
Tossi
A
A
Department of Biochemistry, Biophysics, and Macromolecular Chemistry, University of Trieste, Italy.
Scocchi
M
M
Zanetti
M
M
Gennaro
R
R
Storici
P
P
Romeo
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D001853:Bone Marrow / Q000378:metabolism; D002352:Carrier Proteins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D054804:Cathelicidins; D002417:Cattle; D002463:Cell Membrane Permeability; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006461:Hemolysis / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011506:Proteins; D013552:Swine
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D002352:Carrier Proteins; D054804:Cathelicidins; D018076:DNA, Complementary; C085474:PMAP-36; D010455:Peptides; D010456:Peptides, Cyclic; D011498:Protein Precursors; D011506:Proteins; C086301:porcine myeloid antibacterial peptide 23; C056454:bactenecin