The use of bacteriophage-based systems for the separation and concentration of Salmonella.
Techniques for the separation/concentration of micro-organisms from background food matrices can be applied to increase the speed of analysis and ease of isolation and detection of target micro-organisms. One recent example of such a technique is the immunomagnetic separation (IMS) procedure that has been used for the separation of specific micro-organisms from foods. This paper describes the use of a novel biosorbent consisting of a Salmonella-specific bacteriophage (phage) immobilized to a solid phase that was used for the separation and concentration of Salmonella from food materials. This work has shown that a Salmonella-specific phage-based biosorbent could remove Salmonella from culture fluid and separate Salmonella from suspensions of other Enterobacteriaceae. The ease of production of phage, high affinity of phage-cell interaction and the ability of phage to infect host cells in heterogeneous environments indicates the potential of such a biosorbent as the basis for a reliable separation system in food microbiological analysis.
Bennett
A R
AR
Microbiology Department, Campden & Chorleywood Food Research Association, Chipping Campden, Glos, UK.
Davids
F G
FG
Vlahodimou
S
S
Banks
J G
JG
Betts
R P
RP
D001431:Bacteriological Techniques; D001435:Bacteriophages; D005516:Food Microbiology; D011137:Polystyrenes; D012475:Salmonella / Q000302:isolation & purification* / Q000821:virology*
D011137:Polystyrenes