title: Spectrophotometric methods for the determination of cephradine or ceftazidine in human urine using batch and flow-injection procedures.
abstract: Sensitive and fast spectrophotometric methods for the determination of cephradine or ceftazidine in human urine, based on the formation of compounds between these drugs and Pd(II), are described. In the batch procedures the calibration graphs resulting from the measurement of the absorbance at 330 nm is linear over the range 5.0-60.0 micrograms. ml-1 for cephradine and 3.0-60.0 micrograms ml-1 for ceftazidine. The methods were successfully adapted to FI-systems, the peak heights being proportional to cephalosporin concentration over the range 5.0-60.0 micrograms ml-1 for cephradine and 3.0-60.0 micrograms ml-1 for ceftazidine. The sampling frequency was 60 h-1 with a sample injection of 72 microliters.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Ródenas
authors.forename: M S
authors.initials: MS
authors.lastname: García
authors.forename: C
authors.initials: C
authors.lastname: Sánchez-Pedreño
authors.forename: M I
authors.initials: MI
authors.lastname: Albero
mesh_terms: D002442:Ceftazidime / Q000652:urine*; D002511:Cephalosporins / Q000652:urine*; D002515:Cephradine / Q000652:urine*; D017022:Flow Injection Analysis; D006801:Humans; D013053:Spectrophotometry
chemical_list: D002511:Cephalosporins; D002442:Ceftazidime; D002515:Cephradine