Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells.
To identify genes expressed by a specific subset of dendritic cells found in vivo a polymerase chain reaction-based cDNA subtraction technique was applied to the recently described germinal center dendritic cells. A novel member of the disintegrin metalloproteinase family was cloned which comprises a not typical zinc-chelating catalytic site most similar to a bacterial metalloproteinase. Dendritic cell precursors or immature dendritic cells express no or low levels of the message. It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction. In situ hybridization showed distinct expression of this gene in the germinal center. This, together with the findings that certain disintegrin metalloproteinases regulate the activity of tumor necrosis factor alpha and that metalloproteinases have also been implicated in FasL processing, suggest that this novel molecule may play an important role in dendritic cell function and their interactions with germinal center T cells.
Mueller
C G
CG
Schering-Plough Laboratory for Immunological Research, 69571 Dardilly, France.
Rissoan
M C
MC
Salinas
B
B
Ait-Yahia
S
S
Ravel
O
O
Bridon
J M
JM
Briere
F
F
Lebecque
S
S
Liu
Y J
YJ
D051722:ADAM Proteins; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018845:CD11 Antigens / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology*; D003001:Cloning, Molecular; D016373:DNA, Antisense; D018076:DNA, Complementary / Q000737:chemistry; D003713:Dendritic Cells / Q000201:enzymology* / Q000276:immunology; D019483:Disintegrins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D018858:Germinal Center / Q000201:enzymology* / Q000276:immunology; D006801:Humans; D017403:In Situ Hybridization; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D014066:Palatine Tonsil; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000737:chemistry
D018845:CD11 Antigens; D019013:CD40 Antigens; D016373:DNA, Antisense; D018076:DNA, Complementary; D019483:Disintegrins; D051722:ADAM Proteins; D008666:Metalloendopeptidases; C108169:decysin