Retroviral particles produced from a stable human-derived packaging cell line transduce target cells with very high efficiencies.
The goal of this work was to determine whether a stable 293 amphotropic packaging line, which we have designated 293-SPA, is useful for the production of high-titer stable virus by comparison to the murine psiCRIP line. Here, we report our unexpected findings that particles derived from the 293-SPA line transduce target cells (both NIH-3T3 cells and primary melanoma cells) with greatly enhanced efficiencies (at least 10-fold) compared to particles derived from the psiCRIP packaging line. We show that the presence of a transferable inhibitor in the psiCRIP line at least partially accounts for this dramatic difference in transduction efficiency. This work has important implications for improving the efficiency of retrovirus-mediated gene transfer in general as well as in the design of new packaging cell lines.
Davis
J L
JL
Somatix Therapy Corporation, Alameda, CA 94010, USA.
Witt
R M
RM
Gross
P R
PR
Hokanson
C A
CA
Jungles
S
S
Cohen
L K
LK
Danos
O
O
Spratt
S K
SK
D016475:3T3 Cells / Q000821:virology; D000818:Animals; D002460:Cell Line; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000196:embryology / Q000821:virology*; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000821:virology; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D001616:beta-Galactosidase