Molecular cloning and functional analysis of an rpoS homologue gene from Vibrio cholerae N86.
A homologue of the rpoS gene of Escherichia coli was cloned from Vibrio cholerae N86 by complementation of the phenotypes of the E. coli rpoS mutant strain. We determined the DNA sequence of this gene. Sequence alignments have indicated that the rpoS gene of V. cholerae N86 encoding a 39-kDa protein is very similar to that of E. coli. In addition, the nlpD-like gene was found in the upstream region of the rpoS gene in the same order as in E. coli. These results suggest that the organization of these genes is highly conserved between E. coli and V. cholerae.
Hiratsu
K
K
Department of Molecular Microbiology, Osaka University, Japan.
Yamamoto
K
K
Makino
K
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D015723:Gene Library; D005816:Genetic Complementation Test; D008074:Lipoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism*; D014734:Vibrio cholerae / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C490122:NlpD protein, E coli; C086389:NlpD protein, bacteria; D011994:Recombinant Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases; D001616:beta-Galactosidase