Sex-lethal interactions with protein and RNA. Roles of glycine-rich and RNA binding domains.
Sex-lethal (Sxl) is an RNA-binding protein, containing two conserved RNA binding domains (RBDs) and a glycine-rich region, which functions as a regulator of alternative splicing in Drosophila sex determination. Previous work demonstrated that Sxl monomers interact cooperatively upon binding to target RNAs and that the cooperativity depends on the glycine-rich N terminus. Here we use band shift experiments to show that RNA binding patterns are altered when Sxl is combined with other proteins having similar glycine-rich domains, including mammalian heterogeneous nuclear (hn) RNP L and Drosophila Hrb87F (an hnRNP A/B homolog). Direct involvement of the Sxl glycine-rich region in protein interactions was verified by Far-Western analysis. Two interaction domains, the Sxl N terminus and the Sxl first RNA binding domain, were suggested by the yeast two-hybrid assay. In a systematic examination of the RNA binding properties of Sxl domains, it was found that the Sxl termini as well as the RBDs influence RNA binding specificity. Finally, selection of the Sxl optimal binding site (SELEX) confirms the importance of U-runs in the Sxl binding site and suggests a second type of non-U-run target that may be associated with RNA secondary structure.
Wang
J
J
Molecular Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-1340, USA.
Dong
Z
Z
Bell
L R
LR
D000818:Animals; D001665:Binding Sites; D004330:Drosophila; D029721:Drosophila Proteins; D005998:Glycine / Q000378:metabolism*; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D007301:Insect Hormones / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012332:RNA, Heterogeneous Nuclear / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism
D029721:Drosophila Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D007301:Insect Hormones; D009363:Neoplasm Proteins; D012332:RNA, Heterogeneous Nuclear; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C067777:Sxl protein, Drosophila; D012313:RNA; D005998:Glycine