[Studies on MDM2 oncogene expression and its effect on pancreatic carcinoma cells].
In order to study the interrelation and interaction between MDM2 oncogene and wild type p53 in human pancreatic cancer, we studied the expression and amplification of MDM2 oncogene and its antagonistic effect on wild type p53 by use of gene recombination, gene transduction and molecular hybridization techniques. The results showed that MDM2 oncogene could be detected in all 5 pancreatic cell lines, but MDM2 mRNA expression varied in the different cell lines. The recombinant vector pCMV-MDM2 was transduced into PC-2/s-wtp53 cell line (a transformed PC-2 pancreatic carcinoma cell line containing wild type p53 gene). The resultant cell line, PC-2/s-wtp53/pCMV-MDM2 showed rapid cell growth, a rate similar to that of the parent cell line PC-2. Our results verify the fact that MDM2 gene can abrogate the cell growth arrest mediated by wild type p53 and the antagonistic function of wild type p53.
Guo
H
H
Department of Pathology, PUMC Hospital, CAMS, Beijing.
Liu
T
T
Gao
J
J
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000818:Animals; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009687:Nuclear Proteins; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D051736:Proto-Oncogene Proteins c-mdm2; D012333:RNA, Messenger / Q000096:biosynthesis; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C497206:MDM2 protein, human; C497207:Mdm2 protein, mouse; D051736:Proto-Oncogene Proteins c-mdm2