Modification of polystyrenic matrices for the purification of proteins. III. Effects of poly(vinyl alcohol) modification on the characteristics of protein adsorption on conventional and perfusion polystyrenic matrices.
Poly(styrene-divinylbenzene) (PS-DVB) chromatography matrices, CG1000sd 20-50 microns (TosoHaas), PLRP4000s 15-25 microns, PLRP4000s 50-70 microns (Polymer Laboratories) have been modified by the adsorption and crosslinking of poly(vinyl alcohol) (PVA) to create a matrix suitable for the attachment of dye ligands. The adsorption capacities of lysozyme and HSA on these Procion Yellow HE-3G dyed PVA modified PS-DVB matrices were measured at various flow-rates and the capacities were compared with a Procion Yellow HE-3G dyed OH-activated POROS 20, 20-micron matrix (PerSeptive Biosystems). The adsorption of small proteins was not hindered by the smaller pores of the CG1000sd beads, but as protein size increased, and at high flow-rates, a high mass transfer rate became more dependent on large pore size and small particle diameter.
Nash
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Department of Chemical Engineering, University of Cambridge, UK.
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D000327:Adsorption; D002845:Chromatography; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D007700:Kinetics; D009113:Muramidase / Q000302:isolation & purification; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000302:isolation & purification; D014753:Vinyl Compounds
D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D011137:Polystyrenes; D011506:Proteins; D014227:Triazines; D014753:Vinyl Compounds; C009794:procion yellow; D011142:Polyvinyl Alcohol; D009113:Muramidase; C004985:divinyl benzene