A high level of accumulation of 2-hydroxymuconic 6-semialdehyde from aniline by the transpositional mutant Y-2 of Pseudomonas species AW-2.
Four transpositional mutants of aniline-assimilating Pseudomonas sp. AW-2 produced 2-hydroxymuconic 6-semialdehyde (HMS) from aniline and accumulated it in a cultural medium. Among the four mutants, strain Y-2 produced the greatest amount of HMS (0.77 mg/ml) from 1 mg/ml of aniline hydrochloride in a 15-h growing culture. The conversion rate of aniline to HMS was 70% on a molar basis. Resting cells of strain Y-2 produced 0.65 mg/ml of HMS during 4h of incubation in a reaction mixture containing 1 mg/ml of aniline hydrochloride (conversion rate, 60%). Transposon Tn5-Mob was found to be inserted into the gene of HMS dehydrogenase in strain Y-2 by sequence analysis.
Aoki
K
K
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan.
Kodama
N
N
Murakami
S
S
Shinke
R
R
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000445:Aldehyde Oxidoreductases; D000595:Amino Acid Sequence; D000814:Aniline Compounds / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
D000814:Aniline Compounds; D005231:Fatty Acids, Unsaturated; C002091:hydroxymuconic semialdehyde; D000429:Alcohol Oxidoreductases; D000445:Aldehyde Oxidoreductases; C023320:2-hydroxymuconic semialdehyde dehydrogenase; C023650:aniline