title: FtsN, a late recruit to the septum in Escherichia coli.
abstract: The localization of FtsN in Escherichia coli was inves tigated by immunofluorescence microscopy. FtsN is an essential cell division protein with a simple bitopic topology, a short N-terminal cytoplasmic segment fused to a large carboxy periplasmic domain through a single transmembrane domain. FtsN was found to localize to the septum in a ring pattern similar to that observed for FtsZ and FtsA, although the frequency of cells with rings was less. A MalG-FtsN fusion was also localized to the septum, indicating that the information for FtsN localization is supplied by its periplasmic domain. FtsN localization was dependent upon the prior localization of FtsZ and FtsA and required the function of FtsI and FtsQ. Consistent with FtsN functioning after FtsZ, Z rings were observed in a mutant depleted of FtsN.
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA.
authors.forename: S G
authors.initials: SG
authors.lastname: Addinall
authors.forename: C
authors.initials: C
authors.lastname: Cao
authors.forename: J
authors.initials: J
authors.lastname: Lutkenhaus
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D029968:Escherichia coli Proteins; D019084:Fluorescent Antibody Technique, Indirect; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C098711:FtsN protein, E coli; D008565:Membrane Proteins