title: Scaffolds for engineering novel binding sites in proteins.
abstract: The combination of combinational protein chemistry and powerful selection techniques has resulted in the development of novel protein ligands based on the randomization of surface residues of a parental protein which is used as a scaffold. Such binding proteins, selected from libraries via specific binding towards a given target ligand, have the potential to replace natural antibodies in various biotechnological applications.
authors.affiliation: Department of Biochemistry and Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden. perake@biochem.kth.se
authors.forename: P A
authors.initials: PA
authors.lastname: Nygren
authors.forename: M
authors.initials: M
authors.lastname: Uhlén
mesh_terms: D001665:Binding Sites; D008024:Ligands; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D008024:Ligands; D019151:Peptide Library; D011506:Proteins