ZBP-89, a Krüppel-type zinc finger protein, inhibits cell proliferation.
ZBP-89 is a Krüppel-type zinc finger transcription factor that binds to GC-rich sequences. Overexpression of this factor prevents EGF induction of the gastrin promoter; therefore, we postulated that ZBP-89 may modulate cellular proliferation. To test this hypothesis, ZBP-89 was overexpressed in immortalized (GH4) and malignant (AGS) cell lines. Growth parameters, e.g., 3H-thymidine, BrdU labeling, flow cytometry and ornithine decarboxylase promoter activity were analyzed. The results show that DNA synthesis is inhibited and progression to S phase is blocked in GH4 cells. Collectively, these studies demonstrate that ZBP-89 inhibits cellular proliferation at least in part through its ability to bind and repress ornithine deacarboxlyase promoter activity.
Remington
M C
MC
Department of Internal Medicine and Physiology, University of Michigan, Ann Arbor, Michigan, 48109, USA.
Tarlé
S A
SA
Simon
B
B
Merchant
J L
JL
D000230:Adenocarcinoma / Q000473:pathology; D001667:Binding, Competitive; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D009955:Ornithine Decarboxylase / Q000235:genetics; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers / Q000502:physiology*
D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D009955:Ornithine Decarboxylase