title: Identification of glucuronan lyase from a mutant strain of Rhizobium meliloti.
abstract: The Rhizobium meliloti M5N1CS (NCIMB 40472) mutant strain wich induces nodule formation on alfalfa roots, produces a (1 --> 4)-beta-D-glucuronan partially acetylated. During fermentation under specific conditions, the molecular weight of the polymer decrease, the presence of polysaccharide degrading enzyme was suspected. A glucuronan lyase was identified, this new bacterial lyase produces d.p. 4 oligoglucuronans, substituents (acetates) present on the substrate reduced the enzyme activity.
authors.affiliation: LBM, IUT d'Amiens, France.
authors.forename: P
authors.initials: P
authors.lastname: Michaud
authors.forename: P
authors.initials: P
authors.lastname: Pheulpin
authors.forename: E
authors.initials: E
authors.lastname: Petit
authors.forename: J P
authors.initials: JP
authors.lastname: Séguin
authors.forename: J N
authors.initials: JN
authors.lastname: Barbotin
authors.forename: A
authors.initials: A
authors.lastname: Heyraud
authors.forename: B
authors.initials: B
authors.lastname: Courtois
authors.forename: J
authors.initials: J
authors.lastname: Courtois
mesh_terms: D000107:Acetylation; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000201:enzymology* / Q000235:genetics*
chemical_list: D002241:Carbohydrates; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C088556:mucoric acid; D011133:Polysaccharide-Lyases; C109209:glucuronan lyase