title: A transgenic mouse strain expressing four drug-selectable marker genes.
abstract: Murine embryonic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (MEFs). Because gene targeting experiments often involve sequential selection for multiple-drug resistance in single ES cell lines, we have developed a new mouse strain which represents an economical donor for the production of multiple-drug resistant MEFs. MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Tucker
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Dausman
authors.forename: R
authors.initials: R
authors.lastname: Jaenisch
mesh_terms: D000818:Animals; D015415:Biomarkers; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005347:Fibroblasts; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D015415:Biomarkers