The effect of methyl linoleate hydroperoxides and radical initiator 2,2'-azobis (2-amidinopropane) dihydrochloride on antibody production by mouse spleen cells.
Methyl linoleate hydroperoxides (MLHPO) and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH), a widely used free radical initiator, were examined for effects on antibody production by spleen cells using plaque-forming cell response against sheep red blood cells (SRBC). The in vitro primary antibody response was enhanced in the presence of MLHPO at a concentration range of 2-20 microM or AAPH at 0.1 microM, but was suppressed with higher concentrations of these compounds. In the in vitro secondary antibody responses, both MLHPO and AAPH failed to increase plaque-forming cell response above that of the control culture. Following the oral administration of MLHPO (2.29 g/kg) four times to mice, in vivo primary plaque-forming cell response was significantly suppressed. After, a single intraperitoneal injection of AAPH (60 mg/kg) to mice, in vitro primary plaque-forming cell response was also suppressed. These findings suggest that primary antibody response can be affected by lipid hydroperoxides and oxygen radicals in vivo.
Oarada
M
M
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan.
Kurita
N
N
Miyaji
M
M
D000578:Amidines / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009153:Mutagens / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
D000578:Amidines; D008054:Lipid Peroxides; D009153:Mutagens; C008464:methyl linoleate hydroperoxide; C046728:2,2'-azobis(2-amidinopropane)