Micellar electrokinetic chromatography as a generalized alternative to high-performance liquid chromatography for purity determination of a class of investigational antibacterial drugs.
Micellar electrokinetic chromatography (MEKC) was successfully used to provide purity data for a number of oxazolidinone antibacterials. A run buffer of 100 mM SDS and 40 mM HEPES (pH 7.5, NaOH) separated fifteen different materials of neutral and cationic, anionic or zwitterionic character, usually with efficiencies ten-fold of those observed for HPLC. Different HPLC conditions were required for compounds with different structural characteristics. While the high efficiency and finite migration window of MEKC may allow observation of impurities not seen by HPLC, general use of this method for purity screening of combinatorial compounds will require micellar solutions tolerant of high amounts of organic, in order to accommodate materials of low aqueous solubility.
Scholl
J P
JP
Pharmacia and Upjohn, Inc., Kalamazoo, MI 49001-0199, USA.
DeZwaan
J
J
D000890:Anti-Infective Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D015507:Drugs, Investigational / Q000032:analysis*; D019075:Electrophoresis, Capillary; D010080:Oxazoles / Q000032:analysis*
D000890:Anti-Infective Agents; D015507:Drugs, Investigational; D010080:Oxazoles