Pulsed-field gel electrophoresis analysis of clonal relationships among Neisseria meningitidis A strains from different outbreaks.
Pulsed-field gel electrophoresis was used to analyze the DNA of 67 Neisseria meningitidis A strains from five African outbreaks occurring between 1988 and 1996. Endonuclease Bg/II was used to cut chromosomal DNA, generating 19 analyzable fragments. This technique allowed comparison of clonal relationships between outbreak strains and revealed that the same pulsotype was responsible for the outbreaks. This pulsotype is closely related to strain B54, subgroup III reference strain, Finland, 1975, showing only two fragment differences.
Nicolas
P
P
WHO Collaborating Center for Reference and Research on Meningococci, Institut de Médecine Tropicale du Service de Santé des Armées, Marseille Armées, France.
Parzy
D
D
Martet
G
G
D000349:Africa / Q000453:epidemiology; D015373:Bacterial Typing Techniques; D002999:Clone Cells / Q000145:classification; D004269:DNA, Bacterial / Q000032:analysis*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008585:Meningitis, Meningococcal / Q000453:epidemiology* / Q000382:microbiology; D008589:Meningococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics*; D012703:Serotyping
D004269:DNA, Bacterial