Modification of polystyrenic matrices for the purification of proteins. II. Effect of the degree of glutaraldehyde-poly(vinyl alcohol) crosslinking on various dye ligand chromatography systems.
A poly(styrene-divinylbenzene) chromatography matrix, CG1000sd (TosoHaas) has been modified by the adsorption and crosslinking of poly(vinyl alcohol) (PVA) to create a matrix suitable for the attachment of dye ligands for the adsorption of lysozyme. However, it is shown that there was limited recovery and repeated drops in capacity with adsorption of human serum albumin (HSA). The effect of changing the nature of the PVA crosslinking on the HSA binding characteristics was studied, as well as the effect of using differing dye ligands. The total amount of irreversible HSA binding decreased with greater crosslinking and there were large differences in HSA adsorption characteristics between differing dye types.
Nash
D C
DC
Department of Chemical Engineering, University of Cambridge, UK.
Chase
H A
HA
D000327:Adsorption; D055598:Chemical Phenomena; D002621:Chemistry; D002853:Chromatography, Liquid; D004396:Coloring Agents; D003432:Cross-Linking Reagents; D005976:Glutaral / Q000737:chemistry*; D008024:Ligands; D009113:Muramidase / Q000302:isolation & purification; D011137:Polystyrenes / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D014753:Vinyl Compounds / Q000737:chemistry
D004396:Coloring Agents; D003432:Cross-Linking Reagents; D008024:Ligands; D011137:Polystyrenes; D011506:Proteins; D014753:Vinyl Compounds; D011142:Polyvinyl Alcohol; D009113:Muramidase; C004985:divinyl benzene; D005976:Glutaral