DNA typing of HLA-B27 by polymerase chain reaction.
To find a specific method for HLA-B27 typing, we tested an HLA-B27-specific polymerase chain reaction. This method was used for screening 100 randomly selected blood donors, 10 of them being HLA-B27 positive. A flow cytometric method and this PCR method were compared.
Lucotte
G
G
Laboratory of Neurogenetics, Maison Blanche Hospital, Reims, 51092, France.
Burckel
A
A
D005434:Flow Cytometry / Q000191:economics / Q000379:methods; D015796:HLA-B27 Antigen / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015796:HLA-B27 Antigen