title: Ubiquinol-10 and total peroxyl radical trapping capacity of LDL lipoproteins during aging: the effects of Q-10 supplementation.
abstract: Evidence is rapidly accumulating that oxidative modification of low density lipoprotein (LDL) may play an important role in the pathogenesis of atherosclerosis. In this study we measured the total peroxyl radical trapping capacity of human plasma LDL phospholipids (TRAPLDL) with a luminescent method. The study was carried out with 70 healthy volunteers, aged 28-77. In males an age-related decrease in TRAPLDL was observed. In the age group under 50 years the mean TRAPLDL was 31.36 +/- 1.45 pmol peroxyl radicals/nmol Pi; among those over 50 years it was significantly lower at 26.67 0.94 pmol/nmol Pi. As regards the components of TRAPLDL, the concentration of LDL-ubiquinol did not change and a non-significant decrease in the LDL-tocopherol concentration was detected with age. In females, the mean TRAPLDL, LDL-ubiquinol-10 and tocopherol concentrations did not differ between the age groups. When 17 of the participants were given coenzyme Q10 (Q10) supplementation, 100 mg/day, a highly significant increase in LDL-ubiquinol concentration was detected. Our results indicate that LDL antioxidant defenses tend to decrease with age in the Finnish male population. The decline is most significant in males under 50 years; in older age groups the values remain stable at a low level. Q10 supplementation doubles the number of ubiquinol-10-containing LDL molecules and may therefore have an inhibitory effect on LDL oxidation.
authors.affiliation: Department of Pharmacology, Clinical Pharmacology and Toxicology, Medical School, University of Tampere, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Aejmelaeus
authors.forename: T
authors.initials: T
authors.lastname: Metsä-Ketelä
authors.forename: P
authors.initials: P
authors.lastname: Laippala
authors.forename: T
authors.initials: T
authors.lastname: Solakivi
authors.forename: H
authors.initials: H
authors.lastname: Alho
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood*; D000975:Antioxidants / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D005387:Finland; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D010545:Peroxides / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D012737:Sex Factors; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood; D014810:Vitamin E / Q000097:blood
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D010545:Peroxides; D010743:Phospholipids; D014451:Ubiquinone; D014810:Vitamin E; C049375:perhydroxyl radical; C026663:ubiquinol-10; D002784:Cholesterol