title: Identification and expression of epidermal growth factor gene in mouse testis.
abstract: Epidermal growth factor (EGF) is produced primarily by Leydig cells of human testis. Expression of the EGF gene was assessed in mouse testis during the course of sexual maturation by the application of the RT-PCR method and the use of specific oligonucleotide primers. Testis EGF mRNA content increased with the developmental age of the mice, i.e., day 15 < day 30 < day 45 postnatal. The expression of the EGF gene appears to correlate with maturation of the testis and proliferation of Leydig-cells.
authors.affiliation: Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China.
authors.forename: M L
authors.initials: ML
authors.lastname: Zhang
authors.forename: Y C
authors.initials: YC
authors.lastname: Yan
authors.forename: Y P
authors.initials: YP
authors.lastname: Sun
authors.forename: S S
authors.initials: SS
authors.lastname: Koide
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D018076:DNA, Complementary / Q000235:genetics; D004815:Epidermal Growth Factor / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005796:Genes / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D013737:Testis / Q000737:chemistry / Q000254:growth & development*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D004247:DNA