title: Hypofractionated radiation induces a decrease in cell proliferation but no histological damage to organotypic multicellular spheroids of human glioblastomas.
abstract: The aim of this study was to examine the effect of radiation on glioblastoma, using an organotypic multicellular spheroid (OMS) model. Most glioblastoma cell lines are, in contrast to glioblastomas in vivo, relatively radiosensitive. This limits the value of using cell lines for studying the radiation effect of glioblastomas. The advantage of OMS is maintenance of the characteristics of the original tumour, which is lost in conventional cell cultures. OMS prepared from four glioblastomas were treated with hypofractionated radiation with a radiobiologically equivalent dose to standard radiation treatment for glioblastoma patients. After treatment, the histology as well as the cell proliferation of the OMS was examined. After radiation, a significant decrease in cell proliferation was found, although no histological damage to the OMS was observed. The modest effects of radiation on the OMS are in agreement with the limited therapeutic value of radiotherapy for glioblastoma patients. Therefore, OMS seems to be a good alternative for cell lines to study the radiobiological effect on glioblastomas.
authors.affiliation: Department of (Neuro)Pathology, Academic Medical Center, Amsterdam, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Kaaijk
authors.forename: D
authors.initials: D
authors.lastname: Troost
authors.forename: P
authors.initials: P
authors.lastname: Sminia
authors.forename: M C
authors.initials: MC
authors.lastname: Hulshof
authors.forename: A H
authors.initials: AH
authors.lastname: van der Kracht
authors.forename: S
authors.initials: S
authors.lastname: Leenstra
authors.forename: D A
authors.initials: DA
authors.lastname: Bosch
mesh_terms: D002455:Cell Division / Q000528:radiation effects; D005909:Glioblastoma / Q000737:chemistry / Q000473:pathology / Q000532:radiotherapy*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D011829:Radiation Dosage; D018874:Spheroids, Cellular / Q000737:chemistry / Q000473:pathology / Q000528:radiation effects*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D016159:Tumor Suppressor Protein p53