title: Development of a nanoscale liquid chromatography/electrospray mass spectrometry methodology for the detection and identification of DNA adducts.
abstract: In this work, the coupling of liquid nanochromatography to NanoFlow electrospray mass spectrometry was evaluated for the detection of DNA adducts. The NanoFlow ES LC/MS system was compared with the capillary and conventional ES LC/MS system by analyzing an in vitro reaction mixture resulting from the interaction of 2'-deoxyguanosine 5'-monophosphate with bisphenol A diglycidyl ether and by injecting 2'-deoxyadenosine. By using NanoFlow ES LC/MS, the mass sensitivity could be improved by a factor of 3300. Three different injection methods used in liquid nanochromatography, i.e., split, large-volume, and column-switching injections were compared in terms of sensitivity. Furthermore, NanoFlow ES LC/MS was used to detect 2'-deoxynucleotide adducts isolated from an in vitro mixture of calf thymus DNA and bisphenol A diglycidyl ether. Different 2'-deoxynucleotide adducts could be identified by monitoring typical product ions, diagnostic for the adducts.
authors.affiliation: Department of Chemistry, University of Antwerp (RUCA-UIA), Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Vanhoutte
authors.forename: W
authors.initials: W
authors.lastname: Van Dongen
authors.forename: I
authors.initials: I
authors.lastname: Hoes
authors.forename: F
authors.initials: F
authors.lastname: Lemière
authors.forename: E L
authors.initials: EL
authors.lastname: Esmans
authors.forename: H
authors.initials: H
authors.lastname: Van Onckelen
authors.forename: E
authors.initials: E
authors.lastname: Van den Eeckhout
authors.forename: R E
authors.initials: RE
authors.lastname: van Soest
authors.forename: A J
authors.initials: AJ
authors.lastname: Hudson
mesh_terms: D001559:Benzhydryl Compounds; D002853:Chromatography, Liquid / Q000379:methods*; D018736:DNA Adducts / Q000032:analysis*; D004852:Epoxy Compounds; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D018736:DNA Adducts; D004852:Epoxy Compounds; C019273:bisphenol A diglycidyl ether; D000098826:Bisphenol A Compounds