title: Hydroquinone-O,O'-diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis.
abstract: When hydroquinone-O,Ooffiacetic acid is used as a linker arm in solid phase oligonucleotide synthesis, the time for NH4OH cleavage of oligodeoxy- or oligoribonucleotides is reduced to only 2 min. This allows increased productivity on automated DNA synthesizers without requiring any other modifications to existing reagents or synthesis and deprotection methods. The Q-linker may also be rapidly cleaved by milder reagents such as 5% NH4OH, potassium carbonate, anhydrous ammonia, t-butylamine or fluoride ion. However, the Q-linker is sufficiently stable for long-term storage at room temperature without degradation and no loss of material occurs during synthesis. The linker is also reasonably resistant to 20% piperidine/DMF, 0.5 M DBU/pyridine and 1:1 triethylamine/ethanol. The Q-linker can therefore serve as a general replacement for both succinyl and oxalyl linker arms.
authors.affiliation: Department of Medical Biochemistry, The University of Calgary, Calgary, Alberta T2N 4N1, Canada. rtpon@acs.ucalgary.ca
authors.forename: R T
authors.initials: RT
authors.lastname: Pon
authors.forename: S
authors.initials: S
authors.lastname: Yu
mesh_terms: D006873:Hydroquinones / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D010070:Oxalates; D013386:Succinates
chemical_list: D006873:Hydroquinones; D009841:Oligonucleotides; D010070:Oxalates; D013386:Succinates; C108371:hydroquinone-O,O'-diacetic acid