Length of resting period between stimulation cycles modulates hemodynamic response to a motor stimulus.
The influence of different lengths of the pre-stimulation resting period on the magnitude of a hemodynamic response evoked by motor stimulation was examined in 10 subjects by means of near-infrared spectroscopy (NIRS). A motor stimulus was used which has been previously established as a model for functional activation studies with NIRS. Subjects performed a 20 s finger opposition task in the hand contralateral to NIRS probe localization over left sensorimotor area (C3', according to the 10-20 system). The duration of the pre-stimulation resting period was varied from 10s to 50s and response magnitude was assessed for each of the interstimulus intervals (10 s, 20 s, 30 s, 40 s and 50 s). Data analysis showed that response magnitude in oxygenated and deoxygenated haemoglobin concentration changed with different interstimulation intervals. Interestingly the greatest NIRS response was obtained with resting period 30 s prior to stimulation; shorter and longer resting periods resulted in smaller responses. The time course and the dependence of response magnitude on interstimulus interval differed between [oxy-Hb] and [deoxy-Hb] changes. For [oxy-Hb] the previously described fast initial increase ('overshoot') and the post-stimulation undershoot was more clearly seen with long prestimulation resting periods. Cytochromeoxidase oxygenation changes did not change significantly with different interstimulus intervals. We conclude that comparisons between different functional activation studies with techniques relying on stimulus evoked changes in cerebral hemodynamics must take into account not only the quality of the experimental paradigm and the length of the stimulation period, but also that the resting period between repetitive stimulations is important for response amplitude and its time course.
Obrig
H
H
Department of Neurology, Charité, Humboldt-Universität zu Berlin, Germany.
Hirth
C
C
Junge-Hülsing
J G
JG
Döge
C
C
Wenzel
R
R
Wolf
T
T
Dirnagl
U
U
Villringer
A
A
D001921:Brain / Q000378:metabolism*; D002560:Cerebrovascular Circulation / Q000502:physiology; D005069:Evaluation Studies as Topic; D006439:Hemodynamics / Q000502:physiology*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D009043:Motor Activity / Q000502:physiology*; D009044:Motor Cortex / Q000098:blood supply / Q000378:metabolism; D010092:Oximetry; D010100:Oxygen / Q000097:blood* / Q000378:metabolism*; D010108:Oxyhemoglobins / Q000378:metabolism; D011597:Psychomotor Performance / Q000502:physiology; D019265:Spectroscopy, Near-Infrared; D013997:Time Factors
D006454:Hemoglobins; D010108:Oxyhemoglobins; C100003:deoxyhemoglobin; D010100:Oxygen