Image analysis of neurofilament immunoreactivity in human spiral ganglion.
Computer-assisted image analysis was used to study the immunoreactivity to NF-L, NF-M and NF-H in human spiral ganglion cells. The concentrations, represented by the relative mean grey of NF-L, NF-M and NF-H, were calculated and compared. The cell-area mean-grey correlations for NF-L, NF-M and NF-H were analysed and calculated, showing that NF-M is more specific to the larger cells (type 1?) and NF-H is more specific for the smaller cells (type 2?), while NF-L is non-specific for cell size. These findings confirm several previous assumptions by providing a quantitative basis. We conclude that image analysis is a useful-even essential-tool for the analysis of immunostained temporal bone sections.
Hsu
W J
WJ
Department of Oto-Rhino-Laryngology, University Hospital, Uppsala, Sweden.
Anniko
M
M
Kubo
T
T
Huang
T S
TS
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D003599:Cytoskeleton / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000276:immunology* / Q000648:ultrastructure; D013136:Spiral Ganglion / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D016900:Neurofilament Proteins