Congenic strains for the blood pressure quantitative trait locus on rat chromosome 2.
A quantitative trait locus (QTL) for blood pressure was previously detected by linkage analysis in a region of rat chromosome 2 using segregating populations derived from crosses of the Dahl salt-sensitive (Dahl S) rat with rats of the Wistar-Kyoto (WKY) strain or Milan normotensive strain (MNS). Two congenic strains, S.WKY-D2N35/Nep and S.MNS-Adh/D2Mit5, have been constructed by replacing a region of chromosome 2 of the Dahl S rat with the homologous region (ie, low blood pressure QTL allele) of either the WKY or MNS rat, respectively. Systolic pressures of congenic strains S.WKY-D2N35/Nep and S.MNS-Adh/D2Mit5 fed a 2% NaCl diet for 24 days were 44+/-4.6 and 29+/-4.5 mm Hg lower, respectively, than that of the comparably treated Dahl S rats. The differences between congenic and Dahl S rats in blood pressure were highly significant (P<.001) and were corroborated by significantly (P<.001) lower ratio of heart weight to body weight in the congenic strains compared with Dahl S rats. The data from two congenic strains combined unequivocally establish the existence of a blood pressure QTL on rat chromosome 2.
Deng
A Y
AY
Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo 43699-0008, USA. adeng@vortex.mco.edu
Dene
H
H
Rapp
J P
JP
D000483:Alleles; D000818:Animals; D001794:Blood Pressure / Q000502:physiology*; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D002889:Chromosomes, Human, Pair 2; D004039:Diet, Sodium-Restricted; D004351:Drug Resistance / Q000235:genetics; D008040:Genetic Linkage; D006321:Heart / Q000033:anatomy & histology; D006801:Humans; D006973:Hypertension / Q000139:chemically induced / Q000235:genetics / Q000503:physiopathology; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics; D011921:Rats, Inbred WKY; D012016:Reference Values; D012965:Sodium Chloride / Q000494:pharmacology
D012965:Sodium Chloride