Facilitated transport of lactate by rat jejunal enterocyte.
L-lactate transport mechanism across rat jejunal enterocyte was investigated using isolated membrane vesicles. In basolateral membrane vesicles L-lactate uptake is stimulated by an inwardly directed H+ gradient; the effect of the pH difference is drastically reduced by FCCP, pCMBS and phloretin, while furosemide is ineffective. The pH gradient effect is strongly temperature dependent. The initial rate of the proton gradient-induced lactate uptake is saturable with respect to external lactate with a K(m) of 39.2 +/- 4.8 mM and a Jmax of 8.9 +/- 0.7 nmoles mg protein-1 sec-1. A very small conductive pathway for L-lactate is present in basolateral membranes. In brush border membrane vesicles both Na+ and H+ gradients exert a small stimulatory effect on lactate uptake. We conclude that rat jejunal basolateral membrane contains a H(+)-lactate cotransporter, whereas in the apical membrane both H(+)-lactate and Na(+)-lactate cotransporters are present, even if they exhibit a low transport rate.
Orsenigo
M N
MN
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Tosco
M
M
Laforenza
U
U
Faelli
A
A
D000818:Animals; D001692:Biological Transport; D003576:Electron Transport Complex IV / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007583:Jejunum / Q000166:cytology / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013696:Temperature; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D019344:Lactic Acid; D003576:Electron Transport Complex IV; D005723:gamma-Glutamyltransferase; D000254:Sodium-Potassium-Exchanging ATPase