Influence of successive and combined ultraviolet A and B irradiations on matrix metalloelastases produced by human dermal fibroblasts in culture.
To study the cumulative influence of UV irradiations on skin matrix alterations, human skin fibroblasts were irradiated successively three-fold, at 24 h intervals, with UVA (3x5J/cm2), UVB (3x8mJ/cm2), UVA plus UVB (3x5J/cm2 and 3x8mJ/cm2) and the levels of 92 kDa gelatinase (pro-MMP9), 72 kDa gelatinase (pro-MMP2) and plasma-membrane elastase type protease were determined, following subsequent 24-h culture in 10% serum-containing medium. UV irradiations had only minor influence (1.4-fold increase for UVB) on secreted levels of pro-MMP2 and decreased the amount of plasma membrane elastase produced by cells. It did however, for UVA and UVB alone, induce a significant increase of 66 kDa activated MMP2 production: 2.5- and 1.7-fold respectively. Such enhancement was not observed when combined irradiations were administered. UV exposure possessed a much higher influence on pro-MMP9 secretion by dermal fibroblast enhancing enzyme levels by 2.5-, 6.5- and 5-fold for UVA, UVB and UVA+UVB, respectively.
Kut
C
C
Biochemistry Department, Faculty of Dental Surgery, University René Descartes Paris V, 1 rue M. Arnoux, Montrouge, 92120, France.
Hornebeck
W
W
Groult
N
N
Redziniack
G
G
Godeau
G
G
Pellat
B
B
D000328:Adult; D001940:Breast / Q000166:cytology; D002478:Cells, Cultured; D017364:Collagenases / Q000378:metabolism*; D017077:Culture Media, Conditioned; D004307:Dose-Response Relationship, Radiation; D000078404:Epidermal Cells; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000528:radiation effects; D018093:Gelatinases / Q000378:metabolism*; D006801:Humans; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D017077:Culture Media, Conditioned; D010196:Pancreatic Elastase; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9