Group 5 determination in Pooideae grass pollen extracts by monoclonal antibody-based ELISA. Correlation with biologic activity.
A solid-phase, monoclonal antibody-based ELISA was set up to quantitate group 5 allergens in pollen extracts of wild and cultivated Pooideae grasses. The method was able to evaluate group 5 concentration in mass units with a sensitivity in the ng/ml range and a practical working range of 1-100 ng/ml. The group 5 ELISA was compared with rocket immunoelectrophoresis for determination of allergen levels in several Phleum pratense extracts, and a very good quantitative correlation was found (r = 0.98; P < 0.0001). A highly significant correlation (r > 0.8) was also obtained in comparing allergenic potency determined by RAST inhibition to group 5 content in several wild and cultivated grass species. The results proved the usefulness of the method in the standardization of Pooideae pollen extracts employed in diagnosis and treatment.
Ramírez
J
J
ALK-Abelló, Alergia a Inmunología Abelló SA, Madrid, Spain.
Obispo
T M
TM
Duffort
D
D
Carpizo
J A
JA
Chamorro
M J
MJ
Barber
D
D
Ipsen
H
H
Carreira
J
J
Lombardero
M
M
D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007114:Immunization / Q000592:standards; D015151:Immunoblotting; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E / Q000032:analysis; D006109:Poaceae / Q000276:immunology*; D011058:Pollen / Q000276:immunology; D011852:Radioallergosorbent Test
D000485:Allergens; D000911:Antibodies, Monoclonal; D000941:Antigens; D007073:Immunoglobulin E