title: Different G proteins mediate somatostatin-induced inward rectifier K+ currents in murine brain and endocrine cells.
abstract: 1. Types of G proteins (G protein alpha-subunit subtypes) which mediate the activation of inward rectifier K+ currents by somatostatin (somatotrophin release-inhibiting factor, SRIF) were determined in cultured locus coeruleus neurones from newborn rats and in AtT-20 cells (a mouse pituitary cell line). 2. The whole-cell patch clamp technique was used together with injection of antibodies against pertussis toxin (PTX)-sensitive G protein alpha-subunits or with injection of antisense (or sense) oligonucleotides against these G proteins. 3. In locus coeruleus neurones, the SRIF-induced activation of inward rectifier K+ currents was inhibited by anti-G alpha i1/G alpha i2 antibody injection, but not by anti-G alpha i3 or by anti-G alpha o/G alpha i3 antibody injection, suggesting that the SRIF response is mediated through G alpha i1 and/or G alpha i2. 4. The SRIF-induced activation of the inward rectifier was suppressed in locus coeruleus neurones after injection of antisense oligonucleotides against G alpha i2, but not by injection of sense oligonucleotides against G alpha i2. Injection of antisense (or sense) oligonucleotides against G alpha i1, G alpha i3 and G alpha O (common) had no effect. These results suggest that G alpha i2 is involved in this SRIF response. 5. In AtT-20 cells, the SRIF-induced activation of inward rectifier K+ currents was suppressed by injection of anti-G alpha i3 antibody, but not by injection of anti-G alpha i1/G alpha i2 antibody. 6. The above results indicate that Gi mediates the SRIF effects on inward rectifier K+ currents. However, different subtypes of Gi are involved in the brain neurones and in the endocrine cells: Gi2 in locus coeruleus neurones and Gi3 in AtT-20 cells.
authors.affiliation: Department of Anatomy and Cell Biology, University of Illinois at Chicago, College of Medicine 60612, USA.
authors.forename: K
authors.initials: K
authors.lastname: Takano
authors.forename: J
authors.initials: J
authors.lastname: Yasufuku-Takano
authors.forename: T
authors.initials: T
authors.lastname: Kozasa
authors.forename: S
authors.initials: S
authors.lastname: Nakajima
authors.forename: Y
authors.initials: Y
authors.lastname: Nakajima
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002460:Cell Line; D004594:Electrophysiology; D019204:GTP-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006727:Hormone Antagonists / Q000494:pharmacology*; D008125:Locus Coeruleus / Q000166:cytology*; D051379:Mice; D008845:Microinjections; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D009490:Neurosecretory Systems / Q000166:cytology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010902:Pituitary Gland / Q000166:cytology; D015221:Potassium Channels / Q000819:agonists / Q000502:physiology*; D024661:Potassium Channels, Inwardly Rectifying; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000494:pharmacology*
chemical_list: D000906:Antibodies; D006727:Hormone Antagonists; D016376:Oligonucleotides, Antisense; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D013004:Somatostatin; D019204:GTP-Binding Proteins