title: Toxins isolated from the venom of the Brazilian coral snake (Micrurus frontalis frontalis) include hemorrhagic type phospholipases A2 and postsynaptic neurotoxins.
abstract: Toxins isolated from the venom of the Brazilian coral snake (Micrurus frontalis frontalis) include hemorrhagic type phospholipases A2 and postsynaptic neurotoxins. Toxicon 35, 1193-1203, 1997.-Two sets of proteins have been purified from the venom of the Brazilian coral snake, Micrurus frontalis frontalis. One set has mol. wts, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), in the 8000-13,000 range and includes some proteins which are toxic to mice and others which are not. These proteins appear to be isoforms of postsynaptic toxins. The other set shows phospholipase A2 (PLA2) activity and the toxic members of this set promote hemorrhage in mice in a manner closely resembling that produced by PLA2s isolated from the venom of the Australian tiger snake (Notechis scutatus scutatus). These PLA2s migrate on SDS-PAGE with apparent mol. wts in the 18,000-22,000 range which is characteristic of PLA2s that have an alpha-helix D similar to pancreatic PLA2s. Elapid venom PLA2s of the type which typically migrate on SDS-PAGE with mol. wts in the 13,000-16,000 range and do not have alpha-helix D have not been detected in M. f. frontalis venom.
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie 82071-3944, USA.
authors.forename: B R
authors.initials: BR
authors.lastname: Francis
authors.forename: N J
authors.initials: NJ
authors.lastname: da Silva Júnior
authors.forename: C
authors.initials: C
authors.lastname: Seebart
authors.forename: L L
authors.initials: LL
authors.lastname: Casais e Silva
authors.forename: J J
authors.initials: JJ
authors.lastname: Schmidt
authors.forename: I I
authors.initials: II
authors.lastname: Kaiser
mesh_terms: D000818:Animals; D004546:Elapid Venoms / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006470:Hemorrhage / Q000139:chemically induced; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009498:Neurotoxins / Q000302:isolation & purification*; D010741:Phospholipases A / Q000302:isolation & purification*; D054467:Phospholipases A2; D014118:Toxins, Biological / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004546:Elapid Venoms; D009498:Neurotoxins; D014118:Toxins, Biological; C053972:micrurus venom; D010741:Phospholipases A; D054467:Phospholipases A2