title: Melanin formation in the inner ear is catalyzed by a new tyrosine hydroxylase kinetically and structurally different from tyrosinase.
abstract: Detergent solubilized extracts of the cochleae of adult gerbils (Meriones unguiculatus) contain a tyrosine hydroxylase activity measurable by the radiometric method of Pomerantz. This activity is not related to Fenton-type reactions, since it is not inhibited by free radical scavengers and is heat and protease sensitive. It does not appear to be related to a peroxidase (EC 1.11.1.7) since it is neither dependent on H2O2, nor inhibited by catalase (EC 1.11.1.6). The involvement of a tyrosine hydroxylase (EC 1.14.16.2) related to catecholamine synthesis is also unlikely, since the activity is highly sensitive to 2-mercaptoethanol and is not increased by addition of tetrahydrobiopterin. The activity in crude inner ear extracts displayed an unusual maturation behaviour, with a slow activation upon aging at 4 degrees C. Fully active enzyme displayed Michaelis-Menten kinetics, with a Km for L-tyrosine of 47 microM. Cochlear tyrosine hydroxylase, but not melanoma tyrosinase (EC 1.14.18.1), was inhibited by o-phenanthroline, and was not dependent on L-DOPA as cofactor for full enzymatic activity. Crude extracts were also able to catalyze L-DOPA oxidation and melanin formation from either L-tyrosine or L-DOPA. The tyrosine hydroxylase, DOPA oxidase and melanin formation activities most probably resided in the same molecule, as suggested by inhibition studies. A tyrosine hydroxylase and melanin formation activity with identical properties was found in primary cultures of stria vascularis melanocytes. Immunochemical evidence confirmed the absence of either the tyrosinase encoded for by the albino locus, or the tyrosinase isoenzyme TRP1, encoded for by the brown locus. Conversely, an immunorreactive band of molecular weight 70 kDa was specifically recognized by a tyrosinase polyclonal antiserum in Western blot experiments. These results prove that melanogenesis in the cochlea, and likely in other extracutaneous locations such as the brain, is catalyzed by enzymatic systems different from, but related to tyrosinase.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, University of Murcia, Espinardo, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Benedito
authors.forename: C
authors.initials: C
authors.lastname: Jiménez-Cervantes
authors.forename: D
authors.initials: D
authors.lastname: Pérez
authors.forename: J D
authors.initials: JD
authors.lastname: Cubillana
authors.forename: F
authors.initials: F
authors.lastname: Solano
authors.forename: J
authors.initials: J
authors.lastname: Jiménez-Cervantes
authors.forename: A M
authors.initials: AM
authors.lastname: Meyer zum Gottesberge
authors.forename: J A
authors.initials: JA
authors.lastname: Lozano
authors.forename: J C
authors.initials: JC
authors.lastname: García-Borrón
mesh_terms: D000818:Animals; D002384:Catalysis; D003051:Cochlea / Q000201:enzymology / Q000378:metabolism*; D005849:Gerbillinae; D007700:Kinetics; D007980:Levodopa / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000201:enzymology / Q000378:metabolism; D008546:Melanoma, Experimental / Q000201:enzymology / Q000473:pathology; D051379:Mice; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008543:Melanins; D014443:Tyrosine; D007980:Levodopa; D014446:Tyrosine 3-Monooxygenase; D014442:Monophenol Monooxygenase