Identification of a 48-kDa prenylated protein that associates with microtubules as 2',3'-cyclic nucleotide 3'-phosphodiesterase in FRTL-5 cells.
In an effort to study the nature of tubulin attachment to membranes, we have previously observed that after blocking prenylation in FRTL-5 thyroid cells, the microtubules become disconnected from the plasma membrane region [Bifulco M. et al. (1983) J. Cell. Physiol. 155, 340-348]. In this study we show that several [3H]mevalonate labeled proteins in FRTL-5 cells associate with membrane and cytoskeleton and, among these, we describe the presence of a 48-kDa prenylated protein, identified by immunoprecipitation as 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), that associates with microtubules. This latter association persists through several polymerization/depolymerization cycles, whereas other prenylated proteins are lost. It is suggested that CNP can be a novel microtubule-associated protein (MAP) and a promising candidate as a membrane anchor for microtubules.
Laezza
C
C
CEOS/CNR and Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università Federico II, Napoli, Italy.
Wolff
J
J
Bifulco
M
M
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D008148:Lovastatin / Q000494:pharmacology; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008970:Molecular Weight; D017368:Protein Prenylation / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology / Q000201:enzymology*
D008869:Microtubule-Associated Proteins; D008148:Lovastatin; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases