Immunohistochemical processing of tissue which allows for embedding in Araldite, and orientation and correlation between light and electron microscopic staining.
This paper describes a simple and efficient method for the immunohistochemical processing of tissue sections on glass slides and the subsequent transfer of the entire section to an Araldite medium. These sections present in one plane can then be orientated and when cut provide the user with whole tissue, free from gaps due to folding. This procedure therefore enables the changes in protein concentration and distribution to be assessed and correlated within and between different cells at the light and electron microscopic level. This procedure may be of use in other microscopic techniques studies.
Oates
P S
PS
Department of Physiology, University of Western Australia, Nedlands, Australia. poates@uniwa.uwa.edu.au
Light
A
A
Thomas
C
C
D000818:Animals; D004853:Epoxy Resins; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008854:Microscopy, Electron; D010796:Phthalic Anhydrides; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D016610:Tissue Embedding
D004853:Epoxy Resins; D010796:Phthalic Anhydrides; C005752:araldite