title: Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus.
abstract: The 5'-terminal genomic region (8597 nt) of little cherry virus (LChV), a mealybug-borne closterovirus, was cloned from double-stranded RNA, and its sequence determined to complete the 16934 nt sequence of the monopartite LChV RNA genome. In the 5' to 3' direction, the sequence encompasses ORF 1a, encoding the conserved replicative domains of methyltransferase and helicase, and ORF 1b, encoding RNA polymerase. ORFs 1a and 1b partially overlap (in O/+1 configuration), and the LChV replicase is probably expressed by ribosomal frameshifting as a fusion product with a molecular mass of 318 kDa. The N-terminal part of the ORF 1a product contains a papain-like cysteine proteinase (PCP) domain with a predicted cleavage site between Gly-619 and Ser-620. The PCP and the upstream protein domains can be aligned with the equivalent parts of the leader proteins encoded by the whitefly-transmitted lettuce infectious yellows and sweet potato sunken vein closteroviruses. Phylogenetic reconstruction based on the aligned RNA polymerase sequences clearly suggests that the aphid-transmissible and whitefly-transmissible closteroviruses represent two distinct evolutionary lineages, with the mealybug-transmissible LChV being the most remote member of the 'whitefly' lineage.
authors.affiliation: Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Protection in Fruit Crops, Dossenheim, Federal Republic of Germany. Wilhelm.Jelkmann@urz.uni-heidelberg.de
authors.forename: W
authors.initials: W
authors.lastname: Jelkmann
authors.forename: B
authors.initials: B
authors.lastname: Fechtner
authors.forename: A A
authors.initials: AA
authors.lastname: Agranovsky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017790:Closterovirus / Q000145:classification / Q000235:genetics* / Q000502:physiology; D016384:Consensus Sequence; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D007313:Insecta / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D014675:Vegetables / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics*; D014779:Virus Replication
chemical_list: D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D004265:DNA Helicases