Renal function and oxygen consumption during bacteraemia and endotoxaemia in rats.
BACKGROUND
The hypothesis that renal failure during septic shock may occur as a result of hypoxia-related cell dysfunction was investigated in two rat models of distributive shock.


METHODS
Pentobarbitone-anaesthetized rats received either a bolus (1 ml) of living Escherichia coli bacteria (hospital-acquired strain, 1 x 10(9) CFU/ml; BA-group, n = 7), or a 1-h infusion of endotoxin (E. coli O127.B8: 8 mg/kg; ET-group, n = 7), or saline to serve as time matched controls (C-group, n = 7).


RESULTS
Urine flow in the BA- and ET-group reached a nadir at 1 h, but thereafter increased and reached values higher than control at 3 h. At this time point, renal oxygen delivery had decreased, in the BA-group mainly due to a fall in arterial oxygen content and in the ET-group to a fall in renal plasma flow (clearance of 131I-hippurate). However, renal oxygen extraction had significantly increased, by 31% in the BA and by 59% in the ET group, while renal oxygen consumption remained the same. Net tubular sodium reabsorption had decreased by 55% in the BA and by 25% in the ET group, due to a fall in glomerular filtration rate (clearance of creatinine). Hence, an excess oxygen consumption was found which was caused neither by an increased renal glucose release nor by the presence of an increased number of leukocytes stuck in the glomeruli. Renal tubular cells showed normal morphology. An indication that proximal tubular function in the BA and ET group remained largely intact were normal ATP levels, absence of urinary glucose, and a normal fractional excretion of sodium. However, since urine flow had increased in shocked rats at 3 h, water appeared selectively lost.


CONCLUSIONS
Our data indicate that in rat models of septic shock renal failure is not caused by cortical hypoxia or a shortage of cellular energy supply.
Heemskerk
A E
AE
Laboratory for Physiology, Vrije Universiteit, Amsterdam, The Netherlands.
Huisman
E
E
van Lambalgen
A A
AA
van den Bos
G C
GC
Hennekes
M
M
Thijs
L G
LG
Tangelder
G J
GJ
D000818:Animals; D016470:Bacteremia / Q000378:metabolism* / Q000503:physiopathology*; D004731:Endotoxins / Q000097:blood*; D004926:Escherichia coli; D007668:Kidney / Q000378:metabolism* / Q000503:physiopathology*; D019344:Lactic Acid / Q000097:blood / Q000378:metabolism; D008297:Male; D009318:Natriuresis; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar
D004731:Endotoxins; D019344:Lactic Acid