title: Characterization of cspB, a cold-shock-inducible gene from Lactococcus lactis, and evidence for a family of genes homologous to the Escherichia coli cspA major cold shock gene.
abstract: Upon temperature downshift, the major cold shock protein CspA is highly induced in Escherichia coli. This protein being conserved in other bacteria, we used a PCR-based approach with a pair of degenerate primers derived from highly conserved regions of the CspA-related proteins to evidence the presence of at least three related genes in Lactococcus lactis. One of them, cspB, was cloned and sequenced. It encodes a 66-residue protein which possesses 60% sequence identity with E. coli CspA. Following a cold shock from 30 to 15 degrees C, the level of the cspB mRNA transcript increased, as shown by Northern blot hybridization. In addition, induction of cspB-directed beta-galactosidase activity was observed. These results indicate that the L. lactis cspB gene is cold shock inducible.
authors.affiliation: Unité de Recherche de Biochimie et Structure des Protéines, Institut National de la Recherche Agronomique, Jouy-en-Josas, France. chapot@biotec.jouy.inra.fr
authors.forename: M P
authors.initials: MP
authors.lastname: Chapot-Chartier
authors.forename: C
authors.initials: C
authors.lastname: Schouler
authors.forename: A S
authors.initials: AS
authors.lastname: Lepeuple
authors.forename: J C
authors.initials: JC
authors.lastname: Gripon
authors.forename: M C
authors.initials: MC
authors.lastname: Chopin
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D006360:Heat-Shock Proteins; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C491179:CspB protein, E coli; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C062260:cold shock protein CS7.4, Bacteria; C077188:cold-shock protein CspB, Bacteria