Effects of growth hormone and insulin-like growth factor-1 on protein metabolism, gut morphology, and cell-mediated immunity in burned rats.
The effects of recombinant human growth hormone (GH) and insulin-like growth factor-1 (IGF-1) were investigated in burned rats. Sprague-Dawley rats were fed exclusively by total parenteral nutrition and were subjected to 20% third-degree scald burns. The rats were then divided into the following three groups: (1) the GH group received GH at a dose of 1 IU.kg-1.d-1 for 2d (n = 10); (2) the IGF group received IGF-1 at a dose of 4 mg.kg-1.d-1 for 2d (n = 19); and (3) the control group received saline (n = 17). Cumulative nitrogen balance increased significantly in the GH (P < 0.01) and IGF (P < 0.01) groups as compared with the control group. There were no differences in nitrogen balance between the GH and IGF groups. Blood glucose was decreased in the IGF group (P < 0.01) and increased in the GH group (P < 0.05) as compared with the control group. The intestinal villus height and wall thickness of the GH and IGF groups were significantly greater than those of the control group. Delayed-type hypersensitivity was enhanced in both the GH and the IGF groups as compared with the control group (both P < 0.01). Furthermore, the increase in the IGF group was significantly greater than that in the GH group (P < 0.05). It was concluded that both GH and IGF-1 improve protein metabolism and immune responsiveness, as well as promote proliferation of the intestinal mucosa.
Shimoda
N
N
First Department of Surgery, School of Medicine, Chiba University, Japan.
Tashiro
T
T
Yamamori
H
H
Takagi
K
K
Nakajima
N
N
Ito
I
I
D000818:Animals; D001774:Blood Chemical Analysis; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D002056:Burns / Q000276:immunology* / Q000378:metabolism*; D004044:Dietary Proteins / Q000187:drug effects* / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000503:physiopathology; D007111:Immunity, Cellular / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007421:Intestine, Small / Q000033:anatomy & histology* / Q000187:drug effects*; D007668:Kidney / Q000033:anatomy & histology; D008297:Male; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D009929:Organ Size; D010179:Pancreas / Q000033:anatomy & histology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D013270:Stomach / Q000033:anatomy & histology; D013950:Thymus Gland / Q000033:anatomy & histology
D001786:Blood Glucose; D004044:Dietary Proteins; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D009584:Nitrogen