A new method of synthesis of fluorescently labelled oligonucleotides and their application in DNA sequencing.
A new approach to the chemical synthesis of oligodeoxynucleotides bearing reporter functional groups at base residues of 3'-end nucleosides is reported. Applications of the 3'-end fluorescently labelled primers for automated DNA sequencing are shown.
Markiewicz
W T
WT
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12, PL-61704 Poznan, Poland. markw@ibch.posnon.pl
Gröger
G
G
Rösch
R
R
Zebrowska
A
A
Markiewicz
M
M
Klotz
M
M
Hinz
M
M
Godzina
P
P
Seliger
H
H
D005456:Fluorescent Dyes; D015336:Molecular Probe Techniques; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*
D005456:Fluorescent Dyes; D009841:Oligonucleotides