title: NG-nitro-L-arginine methyl ester modifies the input function measured by dynamic susceptibility contrast magnetic resonance imaging.
abstract: In rat brain dynamic susceptibility contrast magnetic resonance (MR) images, vessels visible on the same scan plane as the brain tissue were used to measure the characteristics of the input function of the MR contrast agent gadopentetate dimeglumine. MR images were acquired 30 and 60 minutes after intravenous injections of 3 mg/kg and 15 mg/kg NG-Nitro-L-arginine methyl ester (L-NAME) (n = 9). The time of arrival (TOA) and the mean transit time corrected for TOA of the input function were increased by 3 mg/kg or 15 mg/kg L-NAME. The area of the input function was increased by 15 mg/kg L-NAME. In two animals, similar modifications of the input function induced by 20 mg/kg L-NAME were reversed by infusion of sodium nitroprusside. In two other animals, MABP was increased by phenylephrine to a similar extent as in L-NAME experiments, but did not induce the same modifications of the input function, showing that the action of L-NAME on the input function was not simply caused by an effect on MABP. These results show that the input function can be significantly altered by manipulations widely used in cerebrovascular studies. These input function changes have important implications for calculation of cerebral blood flow.
authors.affiliation: Department of Radiology and Medical Imaging, Catholic University of Louvain, Brussels, Belgium.
authors.forename: I
authors.initials: I
authors.lastname: Mottet
authors.forename: M J
authors.initials: MJ
authors.lastname: Quast
authors.forename: D S
authors.initials: DS
authors.lastname: Dewitt
authors.forename: G R
authors.initials: GR
authors.lastname: Hillman
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.forename: D H
authors.initials: DH
authors.lastname: Uhrbrock
authors.forename: J R
authors.initials: JR
authors.lastname: Perez-Polo
authors.forename: T A
authors.initials: TA
authors.lastname: Kent
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D003287:Contrast Media / Q000493:pharmacokinetics*; D004338:Drug Combinations; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019786:Gadolinium DTPA; D007201:Indicator Dilution Techniques; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008536:Meglumine / Q000493:pharmacokinetics*; D008962:Models, Theoretical; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D009942:Organometallic Compounds / Q000493:pharmacokinetics*; D004369:Pentetic Acid / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D003287:Contrast Media; D004338:Drug Combinations; D004791:Enzyme Inhibitors; D009942:Organometallic Compounds; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D009599:Nitroprusside; D010656:Phenylephrine; D008536:Meglumine; D004369:Pentetic Acid; D019786:Gadolinium DTPA; D019331:NG-Nitroarginine Methyl Ester