title: Spread of measles virus through axonal pathways into limbic structures in the brain of TAP1 -/- mice.
abstract: The spread of measles virus into the brain was studied exploiting the olfactory pathway, which represents an important route of neuroinvasion by viruses. The virus was injected into the main olfactory bulb of wild-type mice and mice with disrupted TAP1 gene (TAP refers to the Transporter associated with Antigen Presentation), which codes for products essential for the cell-mediated immune response. Virus invasion was monitored for 4 weeks by immunohistochemistry. The distribution of measles virus was found to be restricted to brain areas connected with the olfactory bulbs. However, in the wild-type mice there was a marked infiltration of lymphocytes in the infected brain structures, and the virus did not pass beyond the piriform cortex. In the TAP1 -/- mice the virus spread more extensively along olfactory projections into the limbic system and monoaminergic brainstem neurons. Infected mice of both types developed seizures, which may have been focally evoked from the piriform cortex. This study provides evidence that measles virus can spread through axonal pathways in the brain. The findings obtained in the gene-manipulated mice point out that a compromised immune state of the host may potentiate targeting of virus to the limbic system through olfactory projections.
authors.affiliation: Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
authors.forename: E M
authors.initials: EM
authors.lastname: Urbanska
authors.forename: B J
authors.initials: BJ
authors.lastname: Chambers
authors.forename: H G
authors.initials: HG
authors.lastname: Ljunggren
authors.forename: E
authors.initials: E
authors.lastname: Norrby
authors.forename: K
authors.initials: K
authors.lastname: Kristensson
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000276:immunology / Q000502:physiology*; D000818:Animals; D017951:Antigen Presentation; D001370:Axonal Transport; D001369:Axons / Q000276:immunology / Q000473:pathology / Q000821:virology; D001921:Brain / Q000276:immunology / Q000473:pathology / Q000821:virology*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D007111:Immunity, Cellular / Q000235:genetics; D008032:Limbic System / Q000276:immunology / Q000473:pathology / Q000821:virology; D008297:Male; D008457:Measles / Q000276:immunology / Q000473:pathology / Q000821:virology; D008459:Measles virus / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000821:virology; D009833:Olfactory Pathways / Q000276:immunology / Q000473:pathology / Q000821:virology; D012640:Seizures / Q000209:etiology
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D015395:Histocompatibility Antigens Class I; C073119:TAP1 protein, human; C486171:Tap1 protein, mouse