[Collagen degradation products in amniotic fluid].
It was found that amniotic fluid (38-42 Hbd) contains hydroxyproline in concentration about 10 micrograms/ml. Gel filtration and dialysis demonstrated that most of hydroxyproline exists in a form of low molecular weight products. Furthermore, it was found that amniotic fluid contains a protein which eluates during gel filtration in void volume of the column. It gives a positive reaction for hydroxyproline but the absorption spectrum of such product is not characteristic for this amino acid. Furthermore, it is not digested by bacterial collagenase. It allows to conclude that amniotic fluid (38-42 Hbd) does not contain collagenous proteins. Only low molecular weight degradation products were found. Only part of them is susceptible on the action of bacterial collagenase.
Gogiel
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Zakładu Biochemii Akademii Medycznej w Białymstoku.
Bielecki
D A
DA
D000653:Amniotic Fluid / Q000737:chemistry*; D003094:Collagen / Q000032:analysis / Q000737:chemistry; D017364:Collagenases / Q000737:chemistry; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D011247:Pregnancy; D012016:Reference Values
D003094:Collagen; D017364:Collagenases; D006909:Hydroxyproline