The study of the catalytic sites of enzymes using fluorescent compounds.
The use of fluorescent compounds can be a valuable tool to probe the active site of enzymes. Several examples of this approach are discussed, particularly the use of pyridoxal phosphate analogs. The study of protein-protein interactions by means of fluorescent-labeled proteins is also analyzed.
Churchich
J
J
Department of Biochemistry, University of Tennessee, Knoxville 37996-0840, USA.
D000227:Adenine Nucleotides / Q000737:chemistry*; D016880:Anisotropy; D001665:Binding Sites; D011487:Protein Conformation; D011730:Pyridoxal / Q000737:chemistry*; D011731:Pyridoxal Kinase / Q000737:chemistry*; D011736:Pyridoxine / Q000737:chemistry*; D000637:Transaminases / Q000737:chemistry*
D000227:Adenine Nucleotides; D011730:Pyridoxal; D000637:Transaminases; D011731:Pyridoxal Kinase; D011736:Pyridoxine