Evaluation of a new protocol for peripheral blood stem cell collection with the Fresenius AS 104 cell separator.
In this report we analyzed sixty leukapheresis procedures on 35 patients with a new protocol for the Fresenius AS 104. Yields and efficiencies for MNC, CD 34+ cells, and CFU-GM indicate that the new protocol is able to collect large quantities of hemopoietic progenitors. Procedures were performed processing 8.69 +/- 2.8 liters of whole blood per apheresis and modifying 3 parameters: spillover-volume 7 ml, buffy-coat volume 11.5 ml, centrifuge speed 1,500 rpm; blood flow rate was 50 ml/min and the anticoagulant ratio was 1:12. No side effects were observed during apheresis procedures except for transient paresthesia episodes promptly resolved with the administration of calcium gluconate. Yields show a high capacity of the new program to collect on average MNC 17.28 +/- 10.85 x 10(9), CD 34+ 471 +/- 553.5 x 10(6) and CFU-GM 1278.7 +/- 1346.3 x 10(4) per procedure. Separator collection efficiency on average was 49.91 +/- 23.28% for MNC, 55.1 +/- 35.66% for CFU-GM, and 62.97 +/- 23.09% for CD 34+ cells. Particularly interesting are results for MNC yields and CD 34+ efficiency; these results make the new program advantageous or similar to the most progressive blood cell separators and capable to collect a sufficient number of progenitor cells for a graft with a mean of 1.80 +/- 0.98 procedures per patient.
Menichella
G
G
Centro Ricerche per la Manipolazione dei Costituenti Ematici, Rome, Italy.
Lai
M
M
Pierelli
L
L
Serafini
R
R
Vittori
M
M
Ciarli
M
M
Foddai
M L
ML
Puglia
G
G
Mitschulat
H
H
Scambia
G
G
Leone
G
G
Bizzi
B
B
D000293:Adolescent; D000328:Adult; D001781:Blood Component Removal / Q000295:instrumentation*; D002648:Child; D002675:Child, Preschool; D006412:Hematopoietic Stem Cells; D006801:Humans; D008875:Middle Aged