Presence of ERK2 in rat retinal cells.
OBJECTIVE
Extracellular signal-regulated kinase 2 (ERK2) participates in the phosphorylation cascade that is activated in the an early intracellular response to various hormones and growth factors. We examined the expression and distribution of the ERK2 protein and mRNA in the rat retina before and after light exposure.


METHODS
Rats were held on a 12 hr light/dark cycle and their retinas were removed and examined either just before or 2 or 30 min after light exposure. The tissue was processed for Western blotting to evaluate the presence of the protein for ERK2, and for in situ hybridization to evaluate the mRNA of ERK2.


RESULTS
The Western blotting method showed a strong specific staining of a 42 kDa protein band in the retinal samples. This band corresponded to the expected size of p42 MAP kinase (ERK2). In situ hybridization histochemistry showed an intense localization of ERK2 mRNA in the outer nuclear layer (ONL), the inner nuclear layer (INL), and the ganglion cell layer (GCL) of the retina. The intensity and distribution of these signals did not differ among the animals, regardless of exposure to light.


CONCLUSIONS
While ERK2 may be involved in the signal transduction system activated in retinal cells by light exposure, its precise role remains to be defined.
Imaki
J
J
Department of Anatomy, Nippon Medical School, Tokyo, Japan.
Yoshida
K
K
Yamashita
K
K
Onodera
H
H
Harada
T
T
Shinmei
Y
Y
Matsuda
H
H
Yamakawa
A
A
D000818:Animals; D015153:Blotting, Western; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D008027:Light; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D014018:Tissue Distribution
D012333:RNA, Messenger; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1