[The in vitro antitumor responses of murine peritoneal macrophages induced by adenovirus-mediated IL-4 and/or M-CSF gene therapy].
OBJECTIVE
To observe the antitumor responses of murine peritoneal macrophages induced by adenovirus-mediated IL-4 and/or M-CSF gene transfer.


METHODS
The IL-4 gene and/or M-CSF gene was transfected into murine peritoneal macrophages-mediated by adenovirus and the levels of IL-4, M-CSF, TNF, IL-1 and NO in the supernatant of the macrophages and the cytotoxicity of the macrophages to tumor cells were assayed.


RESULTS
The high levels of IL-4 and M-CSF could be detected in the supernatant of macrophages 18 hours after being infected with advenovirus expressing IL-4 or M-CSF. The cytotoxicity of the macrophages engineered to secrete IL-4 or M-CSF increased significantly, and when IL-4 gene and M-CSF gene were cotransfected into the macrophages or the macrophages were co-stimulated with LPS, the cytotoxicity increased even more significantly. The levels of TNF, IL-land NO in the supernatant of macrophages also increased.


CONCLUSIONS
The results demonstrated that transfection of IL-4 and/or M-CSF gene into macrophages could augment their anti-tumor immunity.
Yu
Y
Y
Department of Immunology, Second Military Medical University, Shanghai.
Cao
X
X
Lei
H
H
D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D003602:Cytotoxicity, Immunologic; D005822:Genetic Vectors; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics*; D007939:Leukemia L1210 / Q000473:pathology; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics*; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014162:Transfection; D014407:Tumor Cells, Cultured
D015847:Interleukin-4; D016173:Macrophage Colony-Stimulating Factor