[The significance of detecting Epstein-Barr virus DNA and latent membrane protein I in Hodgkin's disease from a high incidence area of nasopharyngeal carcinoma].
To study the EBV DNA and expression of latent membrane protein I (LMP1) in patients with Hodgkin's disease from high incidence area of nasopharyngeal carcinoma, hot starting PCR and microwave treatment of LSAB immunohistochemistry methods were used to detect EBV DNA and LMP1 from biopsy specimens from 40 cases of Hodgkin's disease (HD) and 20 cases having benign lymph node lesions, randomly selected from a high incidence area of nasopharyngeal carcinoma. EBV DNA was detected in 65.0% of the HD cases and 50.0% of the benign lymph node cases, the difference was not of statistical significance. LMP1 of EBV was detected in 52.5% of HD cases and its expression was restricted to tumor cells. In addition, the detection rates of LMP1 and DNA of EBV in patients under 20 years of age (84.6% and 76.9% respectively) was much higher than that in patients over 20 years of age (56.0% and 40.0% respectively) P < 0.05. The results suggest that there was latent EBV infection in at least half of HD patients, which may have a role in the genesis and development of HD, and HD occurring in youth was more closely correlated with EBV latent infection and LMP1 expression.
Chu
B
B
Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou.
Zhou
M
M
D004279:DNA, Viral / Q000032:analysis*; D006566:Herpesviridae Infections / Q000821:virology; D004854:Herpesvirus 4, Human / Q000235:genetics; D006689:Hodgkin Disease / Q000821:virology*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000032:analysis*; D011897:Random Allocation; D014412:Tumor Virus Infections / Q000821:virology
D004279:DNA, Viral; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins