Specific binding of acidic phospholipids to microtubule-associated protein MAP1B regulates its interaction with tubulin.
Microtubule-associated protein MAP1B, a major neuronal cytoskeletal protein, is expressed highly during the early stage of brain development and is thought to play an important role in brain development. Although it has been shown that MAP1B localizes both in cytosol and particulate fractions, the underlying molecular mechanism in the membrane localization has yet to be elucidated. In the present study, we show that MAP1B purified from young rat brain can bind to acidic phospholipids, such as phosphatidylserine, but not to a neutral phospholipid, phosphatidylcholine. Furthermore, the binding of MAP1B to taxol-stabilized microtubules was inhibited by the addition of phosphatidylserine or phosphatidylinositol. The addition of phosphatidylcholine showed no effect on the binding of MAP1B to the microtubules. A 120-kDa microtubule-binding fragment of MAP1B was also released from microtubules by the addition of acidic phospholipids. Synthetic peptides derived from the C-terminal half of the tubulin-binding domain, but not that corresponding to the N-terminal half, bound to acidic phospholipids specifically. These results suggest that MAP1B binds to biological membranes through its tubulin-binding site, and the binding may play a regulatory role in MAP1B-microtubule interaction.
Yamauchi
E
E
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Mizuhoku, Nagoya 467, Japan.
Titani
K
K
Taniguchi
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000196:embryology; D006863:Hydrogen-Ion Concentration; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D014404:Tubulin / Q000378:metabolism*
D008869:Microtubule-Associated Proteins; D010743:Phospholipids; D014404:Tubulin; C085284:microtubule-associated protein 1B