Interleukin-6 secretion in mice is associated with reduced glucose-6-phosphatase and liver glycogen levels.
Mice bearing interleukin-6 (IL-6)-secreting tumor were used to study the chronic effect of IL-6 on carbohydrate metabolism. Mice were injected with allogeneic tumor cells transduced with the murine IL-6 gene. Serum IL-6 levels were correlated exponentially with tumor weight. Secretion of IL-6 from the developed tumors was associated with decreased food consumption, reduced body weight, and reduced blood glucose levels. Insulin levels did not change, and 2-deoxyglucose uptake was not affected in most tissues examined. A significant increase of 2-deoxyglucose uptake was measured in the liver. Glycogen content in the liver determined 0, 6, 12, and 18 days after tumor inoculation was 42, 23, 12, and 3 mg/g, respectively. The activity of phosphoenolpyruvate carboxykinase was not affected. The activity of glucose-6-phosphatase (G-6-Phase) determined 6, 12, and 18 days after tumor injection was 84, 70, and 50% of G-6-Pase activity in pair-fed mice bearing nonsecreting tumors, respectively. G-6-Pase mRNA levels were markedly reduced due to inhibition of G-6-Pase gene transcriptional rate.
Metzger
S
S
Division of Medicine, Hadassah University Hospital, Jerusalem, Israel.
Goldschmidt
N
N
Barash
V
V
Peretz
T
T
Drize
O
O
Shilyansky
J
J
Shiloni
E
E
Chajek-Shaul
T
T
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000502:physiology; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004435:Eating / Q000502:physiology; D005260:Female; D005354:Fibrosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D005952:Glucose-6-Phosphatase / Q000235:genetics / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D014161:Transduction, Genetic
D001786:Blood Glucose; D007328:Insulin; D015850:Interleukin-6; D006003:Glycogen; D003847:Deoxyglucose; D005952:Glucose-6-Phosphatase