Active-site studies of enzymes by X-ray diffraction methods.
X-Ray diffraction is the only method currently available for the determination of the detailed three-dimensional structure of macromolecules. In this article, the principles behind the use of X-ray crystallography in the study of protein structure are presented. The results obtained in its application to several enzymes are discussed, as well as the limitations of the method.
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Laboratorio de Biofísica Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Chile.
D001665:Binding Sites; D004789:Enzyme Activation; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction