The expression of interleukin-8 receptor in untreated and treated psoriasis.
The expression of IL-8 in psoriasis has been clearly shown with the use of immunocytochemical, RT-PCR and in situ hybridization methods. The presence of its ligand, the IL-8 receptor, has been demonstrated by the RT-PCR technique. We report here a study of the expression of both IL-8 type A and B receptors by immunohistochemical techniques, using one polyclonal and four monoclonal antibodies. By this technique, we found that the neutrophilic granulocytes express the IL-8 type A receptor, whereas the IL-8 type B receptor was present on the keratinocytes. The type B receptor on the keratinocytes was localized in the suprabasal layers of the epidermis. Following therapy, the expression of the IL-8 type B receptor on the keratinocytes was reduced. This could suggest that IL-8 in psoriasis is involved in the disturbed differentiation rather than in proliferation, probably via an autocrine loop.
Beljaards
R C
RC
Department of Dermatology, Academic Hospital Vrije Universiteit, Amsterdam, The Netherlands.
Van Beek
P
P
Nieboer
C
C
Stoof
T J
TJ
Boorsma
D M
DM
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D011565:Psoriasis / Q000378:metabolism* / Q000628:therapy; D011817:Rabbits; D018123:Receptors, Interleukin / Q000032:analysis*; D023062:Receptors, Interleukin-8A; D012867:Skin / Q000737:chemistry
D015703:Antigens, CD; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A