title: Interactions of hydroxyapatite and fluorapatite particles on human osteoarthritis type B synoviocytes: effects on interleukin-1 alpha levels and lipoxygenase pathways.
abstract: We investigated the effects of the biomaterials hydroxyapatite (HAP) and fluorapatite (FAP) on cultured human osteoarthritis type B synoviocytes by analyzing interleukin-1 alpha (IL-1 alpha) production and arachidonic acid metabolism via lipoxygenase pathways. A portion of opsonized particles was endocytosed and was found in numerous phagolysosomes in human synoviocyte cytoplasms. The present study demonstrates that HAP and FAP calcined at 700 degrees C induced a decrease in IL-1 alpha production but markedly decreased the synthesis of lipoxygenase products after 1-month incubation with the particles. This model will allow us to study the possible inflammatory response (arachidonic acid metabolism, proinflammatory cytokines) that can be induced by any biomaterials used in orthopedics.
authors.affiliation: Faculté de Médecine, E.R.S. CNRS 6101, Limoges, France.
authors.forename: B
authors.initials: B
authors.lastname: Liagre
authors.forename: J L
authors.initials: JL
authors.lastname: Charissoux
authors.forename: M J
authors.initials: MJ
authors.lastname: Leboutet
authors.forename: D
authors.initials: D
authors.lastname: Bernache-Assollant
authors.forename: J L
authors.initials: JL
authors.lastname: Beneytout
mesh_terms: D001031:Apatites / Q000737:chemistry / Q000378:metabolism*; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000378:metabolism*; D008084:Lipoxygenase / Q000096:biosynthesis / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010003:Osteoarthritis / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D013583:Synovial Membrane / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D017886:Durapatite; D008084:Lipoxygenase; C025105:fluorapatite