Affinity labels as probes for the study of the nucleotide binding site of enzymes.
Affinity labeling has proved to be a very useful tool for searching important amino acid residues located in active or allosteric sites of enzymes. In this article, the general principles and specific examples of the use of affinity labeling are discussed.
Bazaes
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Departamento de Química, Universidad Metropolitana de Ciencias de la Educación, Santiago, Chile.
D000345:Affinity Labels / Q000737:chemistry*; D001665:Binding Sites / Q000502:physiology; D009711:Nucleotides / Q000737:chemistry*; D011685:Purine Nucleotides / Q000737:chemistry*; D011770:Pyruvate Kinase / Q000737:chemistry*
D000345:Affinity Labels; D009711:Nucleotides; D011685:Purine Nucleotides; D011770:Pyruvate Kinase