title: The sequence of palF, an environmental pH response gene in Aspergillus nidulans.
abstract: To molecularly characterize the influence of external pH in the secretion of enzymes by filamentous fungi, we have cloned and sequenced the palF gene of Aspergillus nidulans (An). An An wild-type (wt) chromosome VII-specific cosmid library was used to transform a palF15 mutant strain. Selection for complementation was done on medium containing beta-glycerolphosphate as the sole Pi source. Two cosmids were identified (W2G08 and W4G12) and further subcloning of one cosmid (W2G08) defined a 5-kb PstI genomic fragment, which fully complements the palF15 mutation. An internal fragment from the genomic clone recognized a single message of approx. 3.5 kb on Northern blot. cDNA clones were obtained from a lambda gt10 cDNA library and sequenced, showing a nucleotide (nt) sequence of 3311 base pairs (bp), with a 828 bp long 5'-untranslated region (UTR). The major open reading frame (ORF) identified in the sequence codes for a putative 775 amino acid (aa) protein, which shares some similarity with two putative ORF products of Saccharomyces cerevisiae (Sc).
authors.affiliation: Department of Genetics, FMRP, São Paulo University, Ribeirão Preto, Brazil.
authors.forename: W
authors.initials: W
authors.lastname: Maccheroni
authors.forename: G S
authors.initials: GS
authors.lastname: May
authors.forename: N M
authors.initials: NM
authors.lastname: Martinez-Rossi
authors.forename: A
authors.initials: A
authors.lastname: Rossi
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics*; D001483:Base Sequence; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; C107929:PALF protein, Emericella nidulans