[The protective effect of nicotinamide on interleukin-1 beta induced damage to rat islets of Langerhans and its mechanism].
OBJECTIVE
To investigate the protective effects of nicotinamide (NA) on newborn rat islets of Langerhans.


METHODS
Newborn rat islets were isolated by digestion of collagenase V. The effects of IL-1 beta and/or NA on insulin release and nitric oxide (NO) production and 3H-thymidine incorporation were studied.


RESULTS
NA had no effects on both accumulated insulin release and insulin release at an acute glucose challenge, but increased 3H-thymidine incorporation. 25 mmol/L of NA reduced the inhibition of accumulated insulin release and 3H-thymidine incorporation induced by 40/ml of IL-1 beta. However. NA was not able to reverse the reduced ability of IL-1 beta-treated islets to respond to an acute glucose challenge. NA dose-dependently reduced NO production induced by IL-1 beta.


CONCLUSIONS
There is the protective effect of NA on IL-1 beta-induced damage to islets of Langerhans. The mechanism of this effect might be related to that NA stimulates islet cells replication and reduces NO production induced by IL-1 beta.
Gao
H
H
Endocrinology Laboratory, Third Clinical School of Beijing Medical University.
Hong
T
T
Li
Q
Q
D000818:Animals; D000831:Animals, Newborn; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D053583:Interleukin-1beta; D007515:Islets of Langerhans / Q000378:metabolism* / Q000473:pathology; D009536:Niacinamide / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis; D010446:Peptide Fragments / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D007375:Interleukin-1; D053583:Interleukin-1beta; D010446:Peptide Fragments; C052994:interleukin-1beta (163-171); D009536:Niacinamide; D009569:Nitric Oxide