Involvement of endothelial PECAM-1/CD31 in angiogenesis.
The adhesive interactions of endothelial cells with each other and the adhesion receptors that mediate these interactions are probably of fundamental importance to the process of angiogenesis. We therefore studied the effect of inhibiting the function of the endothelial cell-cell adhesion molecule, PECAM-1/ CD31, in rat and murine models of angiogenesis. A polyclonal antibody to human PECAM-1, which cross-reacts with rat PECAM-1, was found to block in vitro tube formation by rat capillary endothelial cells and cytokine-induced rat corneal neovascularization. In mice, two monoclonal antibodies against murine PECAM-1 prevented vessel growth into subcutaneously implanted gels supplemented with basic fibroblast growth factor (bFGF). Taken together these findings provide evidence that PECAM-1 is involved in angiogenesis and suggest that the interactions of endothelial cell-cell adhesion molecules are important in the formation of new vessels.
DeLisser
H M
HM
Department of Medicine, University of Pennsylvania Medical Center, Philadelphia 19104-4283, USA. delisser@mail.med.upenn.edu
Christofidou-Solomidou
M
M
Strieter
R M
RM
Burdick
M D
MD
Robinson
C S
CS
Wexler
R S
RS
Kerr
J S
JS
Garlanda
C
C
Merwin
J R
JR
Madri
J A
JA
Albelda
S M
SM
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002478:Cells, Cultured; D003094:Collagen; D003315:Cornea / Q000098:blood supply / Q000187:drug effects; D004338:Drug Combinations; D004727:Endothelium / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D007797:Laminin; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000187:drug effects; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology / Q000502:physiology*; D011509:Proteoglycans; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D000806:Angiotensin-Converting Enzyme Inhibitors; D000911:Antibodies, Monoclonal; D004338:Drug Combinations; D007797:Laminin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; C059485:matrigel; D003094:Collagen