Cloning and expression of an Arabidopsis gene encoding a putative peroxisomal ascorbate peroxidase.
An Arabidopsis 14-3-3 protein, AFT1, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. One of the candidate proteins, APX3, was identified as a putative peroxisomal membrane-bound ascorbate peroxidase. Ascorbate peroxidases are important defense enzymes that protect plant cells from oxidative stress damage. DNA blot analysis indicates that APX3 is encoded by a single-copy gene in the Arabidopsis genome. RNA blot analyses show that APX3 transcript levels increase slightly in response to cold, UV light, and treatments with hydrogen peroxide and paraquat. The activity of APX3 in Arabidopsis may be controlled in two ways: its enzymatic activity through protein-protein interactions and its transcription by transcriptional or posttranscriptional regulation.
Zhang
H
H
Department of Biological Sciences, Texas Tech University, Lubbock 79409, USA.
Wang
J
J
Nickel
U
U
Allen
R D
RD
Goodman
H M
HM
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008565:Membrane Proteins; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000235:genetics; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000302:isolation & purification; D018749:RNA, Plant / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011994:Recombinant Proteins; D010544:Peroxidases; C555294:APX3 protein, Arabidopsis; D060387:Ascorbate Peroxidases; D014446:Tyrosine 3-Monooxygenase; D001205:Ascorbic Acid