Peroxynitrite-induced hemolysis of human erythrocytes and its inhibition by antioxidants.
It was found that human erythrocytes underwent hemolysis when incubated with peroxynitrite at 37 degrees C under air. The extent of hemolysis increased with increasing peroxynitrite concentration and decreasing hematocrit. The peroxynitrite-induced hemolysis was suppressed only partially by a radical scavenging antioxidant such as uric acid and Trolox, a water-soluble vitamin E analogue, but reduced glutathione, N-acetylcysteine and albumin efficiently inhibited the hemolysis. A selenium-containing organic compound, ebselen, also suppressed the hemolysis. On the other hand, nitric oxide and superoxide generated concomitantly from 3-morpholinosydnonimine (SIN-1) did not induce appreciable hemolysis, while it converted hemoglobin to methemoglobin extensively.
Kondo
H
H
Research Centre for Advanced Science and Technology, University of Tokyo, Japan.
Takahashi
M
M
Niki
E
E
D000111:Acetylcysteine / Q000494:pharmacology; D000418:Albumins / Q000494:pharmacology; D000578:Amidines / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D002839:Chromans / Q000494:pharmacology; D004912:Erythrocytes / Q000502:physiology; D005978:Glutathione / Q000378:metabolism; D006400:Hematocrit; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D054833:Isoindoles; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009566:Nitrates / Q000494:pharmacology*; D016566:Organoselenium Compounds / Q000494:pharmacology; D014527:Uric Acid / Q000494:pharmacology; D014810:Vitamin E / Q000378:metabolism
D000418:Albumins; D000578:Amidines; D000975:Antioxidants; D001393:Azoles; D002839:Chromans; D054833:Isoindoles; D009566:Nitrates; D016566:Organoselenium Compounds; D014810:Vitamin E; C039642:peroxynitric acid; D014527:Uric Acid; C042986:ebselen; C002385:linsidomine; C046728:2,2'-azobis(2-amidinopropane); D008981:Molsidomine; D002374:Catalase; D005978:Glutathione; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D000111:Acetylcysteine