title: Evaluation of IgG glycation levels by matrix-assisted laser desorption/ionization mass spectrometry.
abstract: Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has been employed for the evaluation of the glycation level of IgG from healthy subjects and also from well- and badly-controlled diabetic patients. The measurements have been performed on untreated plasma protein fractions. The data obtained have shown that a clear mass increase, originating from non-enzymatic glycation processes, is observed in the case of diabetic patients: for well-controlled ones it is in the range 512-1565 Da, while it becomes 827-4270 Da for badly-controlled diabetic patients. This approach indicates that MALDI mass spectrometry is a highly specific tool that can be employed in the metabolic control of diabetic patients and in studies relating the IgG glycation level to possible immunological impairment.
authors.affiliation: Istituto di Medicina Interna, Malattie del Metabolismo, Padova, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Lapolla
authors.forename: D
authors.initials: D
authors.lastname: Fedele
authors.forename: R
authors.initials: R
authors.lastname: Aronica
authors.forename: M
authors.initials: M
authors.lastname: Garbeglio
authors.forename: M
authors.initials: M
authors.lastname: D'Alpaos
authors.forename: R
authors.initials: R
authors.lastname: Seraglia
authors.forename: P
authors.initials: P
authors.lastname: Traldi
mesh_terms: D000368:Aged; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008875:Middle Aged; D012709:Serum Albumin / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001786:Blood Glucose; D007074:Immunoglobulin G; D012709:Serum Albumin; D005947:Glucose