Myelin basic protein immunoreactivity in the internal capsule of neonates from rats on a low iodine intake or on methylmercaptoimidazole (MMI).
Rats fed on low iodine diets (LIDs) result in a normal circulating level of triiodothyronine (T3), a low level of thyroxine (T4) and an elevated thyroid-stimulating hormone (TSH). These changes are similar to those observed in habitants who live in iodine-deficient areas and different from those observed when the hypothyroidism is produced by goitrogens. To study the effects of LID or goitrogens on the myelin basic protein (MBP) immunoreactivity (MBP-ir) during the myelination of the internal capsule, one group of experimental female rats was fed on an LID, and another group received a standard laboratory diet with methylmercaptoimidazole (MMI) added in the drinking water. Animals fed on a standard laboratory diet and animals fed on an LID supplemented with KI were used as controls. At P10, the MMI treatment has produced a more marked decrease in the surface density of MBP-ir processes with respect to controls than that produced in the LID animals. This decrease was correlated with the cerebral concentrations of triiodothyronine (T3) we found. During the postnatal development, a recovery in the levels of the surface density with respect to controls was observed in both experimental groups. The recovery occurred by P20 in the LID group and by P32 in the MMI rats.
Martinez-Galan
J R
JR
Unidad de Neuroanatomia, Instituto Cajal, Madrid, Spain.
Pedraza
P
P
Santacana
M
M
Escobar del Rey
F
F
Morreale de Escobar
G
G
Ruiz-Marcos
A
A
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D013956:Antithyroid Agents / Q000494:pharmacology*; D001923:Brain Chemistry / Q000187:drug effects; D002452:Cell Count; D005260:Female; D007150:Immunohistochemistry; D007455:Iodine / Q000172:deficiency*; D008713:Methimazole / Q000494:pharmacology*; D004676:Myelin Basic Protein / Q000378:metabolism*; D009412:Nerve Fibers / Q000502:physiology; D011247:Pregnancy; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013963:Thyroid Hormones / Q000378:metabolism
D013956:Antithyroid Agents; D004676:Myelin Basic Protein; D013963:Thyroid Hormones; D008713:Methimazole; D007455:Iodine