title: Effect of technological treatments on digestibility and allergenicity of meat-based baby foods.
abstract: OBJECTIVE
  When suitability prepared according to particular characteristics of hygiene and digestibility, meat is an important food for human weaning. The present knowledge on meat digestibility and allergenicity are not enough to justify removal of meat from a child's diet when there is risk but no clinical evidence of allergy. Based on these considerations, the role of technological treatments on digestibility and allergenicity of meat-based baby foods is considered.
  
  
  METHODS
  Eight children (five males and three females) suffering from atopic dermatitis (AD), aged 3.8 to 7.1 years (mean age 4.86 +/- 1.10 years).
  
  
  METHODS
  An in vitro multienzymatic digestibility assay was used to evaluate proteolysis in meat samples (from four different animal species). The experimental design included raw, steam-cooked (home-made and industrial cooking), homogenized (strained) and freeze-dried meat samples. Skin prick test (SPT) was performed to evaluate positive responses to meat samples (raw, cooked, strained and freeze-dried) from four animal species.
  
  
  RESULTS
  Our data indicate that enzymatic attack is strongly affected by heat treatment as shown in steam-cooked meat samples. On the other hand, blending, homogenization and freeze-drying processes are able to partially reverse the phenomenon. Data on modification of sodium dodecyl sulfate-polyacrylamide gel rlectrophoresis (SDS-PAGE) protein pattern during the multienzymatic assay are reported. Cooking and technological treatments reduce positive responses obtained in SPT.
  
  
  CONCLUSIONS
  Technological treatments improve digestibility and reduce antigenicity of meat products.
authors.affiliation: Laboratory of Toxicology, Institute of Pharmacological Sciences, University of Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Restani
authors.forename: A
authors.initials: A
authors.lastname: Fiocchi
authors.forename: A R
authors.initials: AR
authors.lastname: Restelli
authors.forename: T
authors.initials: T
authors.lastname: Velonà
authors.forename: B
authors.initials: B
authors.lastname: Beretta
authors.forename: M
authors.initials: M
authors.lastname: Giovannini
authors.forename: C L
authors.initials: CL
authors.lastname: Galli
mesh_terms: D000818:Animals; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000209:etiology; D004063:Digestion; D005260:Female; D005512:Food Hypersensitivity; D005524:Food Technology; D005612:Freeze Drying; D006358:Hot Temperature; D006801:Humans; D007223:Infant; D007225:Infant Food; D008297:Male; D008460:Meat