[Gene expression of bFGF and intimal hyperplasia of autologous vein grafts in rats].
OBJECTIVE
To establish a model of autologous vein graft through translating jugular vein to aortic artery in 80 Wistar rats, and detect gene expression of basic fibroblast growth factor (bFGF).


METHODS
In situ hybridization and reverse transcription polymerase chain reaction (RT-PCR) were used.


RESULTS
The expression of bFGF mRNA was more decreased than that of normal artery and vein after transplantation, up to 1 week, the level of bFGF gene expression decreased to the lowest (P < 0.05). Two weeks after transplantation, bFGF transcripts recovered.


CONCLUSIONS
bFGF released from dead and damaged cells of autologous vein graft not only is an important factor that promotes SMC proliferation, but also is a potent stimulator of endothelial regrowth.
Qian
H
H
Department of Vascular Surgery, Renji Hospital, Shanghai.
Zhang
B
B
Zhao
H
H
D000818:Animals; D001011:Aorta / Q000601:surgery*; D002455:Cell Division; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics*; D015870:Gene Expression; D007601:Jugular Veins / Q000473:pathology / Q000637:transplantation*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014182:Transplantation, Autologous
D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2