Apolipoprotein E genotyping by capillary electrophoretic analysis of restriction fragments.
We present the genotyping of apolipoprotein (apo) E by means of restriction fragment analysis of amplified genomic DNA by high-performance capillary electrophoresis and a replaceable non-gel-sieving matrix. This procedure streamlines the genotyping of apo E in large-scale population studies because of the automation and speed of capillary electrophoresis.
de Bellis
G
G
Istituto di Technologie Biomediche Avanzate, Segrate, Italy. debellis@itba.mi.cnr.it
Salani
G
G
Panigone
S
S
Betti
F
F
Invernizzi
L
L
Luzzana
M
M
D001057:Apolipoproteins E / Q000737:chemistry / Q000235:genetics*; D003062:Codon / Q000235:genetics; D004247:DNA / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D015183:Restriction Mapping; D012680:Sensitivity and Specificity
D001057:Apolipoproteins E; D003062:Codon; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases