Alteration of the symbiotic properties of Rhizobium meliloti by plasmid manipulations.
Mutants with symbiotic activity were obtained by random Tn5-Mob mutagenesis of Rhizobium meliloti 41. The place of the Tn5 insertion was localized on a cryptic middle-sized plasmid pRme41a. Mobilization of the labelled plasmid pRme41a::Tn5-Mob into R. meliloti 114, which did not contain such a plasmid led to a complete loss of the nodulation ability of the recipient strain. The Nod-phenotype was a result of a large deletion in the symbiotic (pSym) plasmid of R. meliloti 114.
Radeva
G
G
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Selenska-Pobell
S
S
D009154:Mutation; D010944:Plants / Q000382:microbiology; D016962:Sinorhizobium meliloti / Q000235:genetics*; D013559:Symbiosis / Q000235:genetics*