Decreased prostaglandin E2 synthesis by lens epithelial cells cultured on heparin-surface-modified poly(methyl methacrylate).
OBJECTIVE
To evaluate whether heparin surface modification reduces prostaglandin E2 (PGE2) synthesis by lens epithelial cells (LECs) after intraocular lens (IOL) implantation.


METHODS
Nishi Eye Hospital, Jinshikai Medical Foundation, Osaka, Japan.


METHODS
The prostaglandin E2 (PGE2) concentration was determined in an incubation medium of human cataract LECs cultured on heparin-surface-modified (HSM) poly(methyl methacrylate) (PMMA) plates at 1, 2, 3, and 4 weeks of culture. A medium without heparin served as a control.


RESULTS
The PGE2 concentration was significantly lower in the HSM than in the control medium at 3 and 4 weeks of culture.


CONCLUSIONS
The results are consistent with the clinical observation of significantly decreased inflammation in eyes with HSM IOLs, indicating that such modification increases PMMA's biocompatibility with LECs.
Nishi
O
O
Nishi Eye Hospital, Osaka, Japan.
Nishi
K
K
Imanishi
M
M
Mano
C
C
Yamada
Y
Y
Tada
Y
Y
Shirasawa
E
E
Härfstrand
A
A
D002386:Cataract / Q000378:metabolism; D002452:Cell Count; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005343:Fibrinolytic Agents; D005500:Follow-Up Studies; D006493:Heparin; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D007910:Lenses, Intraocular; D008768:Methylmethacrylates; D008858:Microscopy, Phase-Contrast; D013499:Surface Properties
D005343:Fibrinolytic Agents; D008768:Methylmethacrylates; D006493:Heparin; D015232:Dinoprostone