Expression of mesenger RNA for transforming growth factor-beta 1 in bovine trabecular meshwork.
OBJECTIVE
To investigate the relationship between transforming growth factor-beta 1 (TGF-beta 1) and primary open-angle glaucoma, we have determined whether trabecular tissues have the expression of messenger RNA for TGF-beta 1.


METHODS
Total RNA of 24 newborn bovine trabecular tissue were extracted by Guanidine isothiocyanate method. The TGF-beta 33 plasmid was brought into E. colibacillius HB101 and amplificated. After Bam HI endolase degradation and label with alpha-32p-dATP the RNA was hybridized with the cDNA (complementary DNA) probe and examined by autoradiography.


RESULTS
The presence of mRNA for TGF-beta 1 in bovine trabecular meshwork was confirmed.


CONCLUSIONS
The TGF-beta 1 present in normal aqueous humor must be at least partly derived from the trabecular meshwork. It offered a basis for understanding the relationship between abnormal synthesis, activation and clearance of TGF-beta 1 and the pathogenesis of primary open-angle glaucoma (POAG) in molecular biology.
Yuan
L
L
Department of Ophthalmology, Union Hospital, Tongji Medical University, Wuhan, China.
Wei
H
H
D000818:Animals; D002417:Cattle; D005902:Glaucoma, Open-Angle / Q000209:etiology; D012333:RNA, Messenger / Q000378:metabolism*; D014129:Trabecular Meshwork / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000235:genetics*
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta