1,2-Diacylglycerol hydroperoxides induce the generation and release of superoxide anion from human polymorphonuclear leukocytes.
We examined the effect of 1-palmitoyl-2-linoleoylglycerol (PLG), PLG hydroxide (PLG-OH), and PLG hydroperoxide (PLG-OOH) on the release of superoxide anion from human PMNs monitored by the chemiluminescence generated by the superoxide anion-sensitive reagent, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin++ +-3-one (MCLA). PLG-OOH at low micromolar concentrations stimulated human PMNs whereas PLG and PLG-OH did not. 1,3-Dilinoleoylglycerol hydroperoxide, 1-palmitoyl-2-linoleoylphosphatidylcholine hydroperoxide, and linoleic acid hydroperoxide were much less efficient in stimulating human PMNs than PLG-OOH. The PKC inhibitors, chelerythrine chloride and staurosporine, inhibited the stimulation of PMNs. Possible pathophysiological role of 1,2-diacylglycerol hydroperoxides is discussed.
Yamamoto
Y
Y
Research Center for Advanced Science and Technology, University of Tokyo, Japan. junkan@oxygen.rcast.u-tokyo.ac.jp
Kambayashi
Y
Y
Ito
T
T
Watanabe
K
K
Nakano
M
M
D004075:Diglycerides / Q000494:pharmacology*; D006801:Humans; D007093:Imidazoles; D008054:Lipid Peroxides / Q000494:pharmacology*; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011719:Pyrazines; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
C011439:1,2-diacylglycerol; D004075:Diglycerides; D007093:Imidazoles; D008054:Lipid Peroxides; D010713:Phosphatidylcholines; D011719:Pyrazines; D013481:Superoxides; C063412:2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one; C097357:1-stearoyl-2-linoleoyl-sn-glycerol; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate