Matrix-assisted laser desorption/ionisation mass spectrometry guided purification of human guanylin from blood ultrafiltrate.
The purification of the human peptide hormone guanylin 22-115 from blood ultrafiltrate (hemofiltrate, HF) was achieved using matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) as the assay system. Screening a peptide bank generated from 5000 1 HF guanylin 22-115 was detected by its molecular mass when adequate conditions for MALDI-MS analysis were chosen. The sensitivity was even better than of the established biological assay system. In addition, the susceptibility towards solvents and salts is strongly reduced. 1.2 mg of the peptide hormone was purified from 10% of the starting material.
Schrader
M
M
Lower Saxony Institute for Peptide Research (IPF), Hannover, Germany. 100140,2162@compuserve.com
Jürgens
M
M
Hess
R
R
Schulz-Knappe
P
P
Raida
M
M
Forssmann
W G
WG
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003110:Colonic Neoplasms / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D005768:Gastrointestinal Hormones; D006801:Humans; D008970:Molecular Weight; D045265:Natriuretic Peptides; D010455:Peptides / Q000097:blood*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured; D014462:Ultrafiltration
D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; D010455:Peptides; C072685:guanylin; D006152:Cyclic GMP