title: Immunologic investigations of T-cell regulation of human IgE antibody secretion and allergic responses.
abstract: The pathophysiology of allergic disease is multifactorial, involving an intricate network of interactions among cells, mediators, and cytokines. Substantial progress has been made in defining the role of antigen-specific T cells and cytokines in the regulation of immunoglobulin E (IgE) synthesis and the atopic diseases. The development of antigen-specific T-cell lines and clones has facilitated efforts to characterize human T-cell subsets and their cytokine repertoires. Molecular methods currently available include techniques for the quantitative analysis of cytokine gene expression and secretion from activated T cells ex vivo as well as in tissues. The availability of these newly developed techniques has become essential to the investigation of the pharmacologic regulation of T cells and cytokines both in vitro and in vivo. Future investigations will contribute to our understanding of the differential regulation of T-cell subsets and their relationships to allergic diseases, ultimately leading to a better understanding of the molecular pathogenesis of allergic diseases and the design of more effective therapeutic interventions.
authors.affiliation: Division of Clinical Immunology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Essayan
authors.forename: G
authors.initials: G
authors.lastname: Krishnaswamy
authors.forename: S K
authors.initials: SK
authors.lastname: Huang
mesh_terms: D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000628:therapy; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000276:immunology; D017403:In Situ Hybridization; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D016207:Cytokines; D005938:Glucocorticoids; D010726:Phosphodiesterase Inhibitors; D012333:RNA, Messenger; D007073:Immunoglobulin E