title: Pathology of experimental Ebola virus infection in African green monkeys. Involvement of fibroblastic reticular cells.
abstract: BACKGROUND
  Ebola virus has been responsible for explosive lethal outbreaks of hemorrhagic fever in both humans and nonhuman primates. Previous studies showed a predilection of Ebola virus for cells of the mononuclear phagocyte system and endothelial cells.
  
  
  OBJECTIVE
  To examine the distribution of lesions and Ebola virus antigen in the tissues of six adult male African green monkeys (Cercopithecus aethiops) that died 6 to 7 days after intraperitoneal inoculation of Ebola-Zaire (Mayinga) virus.
  
  
  METHODS
  Tissues were examined histologically, immunohistochemically, and ultrastructurally.
  
  
  RESULTS
  A major novel finding of this study was that fibroblastic reticular cells were immunohistochemically and ultrastructurally identified as targets of Ebola virus infection.
  
  
  CONCLUSIONS
  The role of Ebola virus-infected fibroblastic reticular cells in the pathogenesis of Ebola hemorrhagic fever warrants further investigation. This is especially important because of recent observations indicating that fibroblastic reticular cells, along with the reticular fibers they produce, maximize the efficiency of the immune response.
authors.affiliation: Pathology Division, USAMRIID, Ft Detrick, MD 21702-5011, USA.
authors.forename: K J
authors.initials: KJ
authors.lastname: Davis
authors.forename: A O
authors.initials: AO
authors.lastname: Anderson
authors.forename: T W
authors.initials: TW
authors.lastname: Geisbert
authors.forename: K E
authors.initials: KE
authors.lastname: Steele
authors.forename: J B
authors.initials: JB
authors.lastname: Geisbert
authors.forename: P
authors.initials: P
authors.lastname: Vogel
authors.forename: B M
authors.initials: BM
authors.lastname: Connolly
authors.forename: J W
authors.initials: JW
authors.lastname: Huggins
authors.forename: P B
authors.initials: PB
authors.lastname: Jahrling
authors.forename: N K
authors.initials: NK
authors.lastname: Jaax
mesh_terms: D000311:Adrenal Glands / Q000648:ultrastructure / Q000821:virology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002522:Chlorocebus aethiops / Q000821:virology*; D029043:Ebolavirus / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity* / Q000648:ultrastructure; D005347:Fibroblasts / Q000648:ultrastructure / Q000821:virology*; D019142:Hemorrhagic Fever, Ebola / Q000473:pathology* / Q000635:transmission / Q000662:veterinary; D007150:Immunohistochemistry; D008099:Liver / Q000648:ultrastructure / Q000821:virology; D008168:Lung / Q000648:ultrastructure / Q000821:virology; D008198:Lymph Nodes / Q000648:ultrastructure / Q000821:virology; D008297:Male; D008643:Mesentery / Q000648:ultrastructure / Q000821:virology; D008992:Monkey Diseases / Q000209:etiology / Q000473:pathology* / Q000635:transmission; D014766:Viremia / Q000473:pathology
chemical_list: D000956:Antigens, Viral