Lymphocyte adhesion molecule ligands and extracellular matrix proteins in gliomas and normal brain: expression of VCAM-1 in gliomas.
To identify antigenic differences between gliomas and normal brain, we have immunohistochemically studied the expression of lymphocyte adhesion molecules (ICAM-1, ICAM-2, ICAM-3, VCAM-1, E-selectin and CD58), epidermal growth factor receptor (EGFR) and extracellular matrix proteins (collagen IV, fibronectin, laminin, merosin, tenascin and vitronectin) in these tissues. Gliomas expressed high levels of ICAM-1, CD58 (LFA-3), EGFR, tenascin and vitronectin, whereas only very low levels were detected in normal brain. VCAM-1 expression was detected in 15 out of 25 gliomas but not in normal brain. The presence of VCAM-1 in gliomas was verified by immunoblotting and RNase protection assay, and in glioma cell lines by Northern blotting. Expression of VCAM-1 in gliomas may partially explain lymphocytic infiltration, and anti-VCAM-1 antibodies may be of potential in antibody mixtures for targeted therapy of gliomas.
Mäenpää
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Department of Pathology, University of Helsinki, Finland. Anna.Maenpaa@helsinki.fi
Kovanen
P E
PE
Paetau
A
A
Jäáskeläinen
J
J
Timonen
T
T
D000328:Adult; D000368:Aged; D015152:Blotting, Northern; D015153:Blotting, Western; D001923:Brain Chemistry / Q000502:physiology*; D001932:Brain Neoplasms / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002648:Child; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D008297:Male; D008875:Middle Aged; D009457:Neuroglia / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1