Cryptosporidiosis-induced impairment of ion transport and Na+-glucose absorption in adult immunocompromised mice.
Electrolyte transport was investigated during chronic cryptosporidiosis in adult anti-interferon-gamma-treated SCID mice by means of Ussing chamber techniques. In basal conditions, infection of immunocompromised mice with Cryptosporidium parvum resulted in a 30% reduction (P < .05) in the ileal short-circuit (Isc) current related to a 28% reduction (P < .05) in tissue conductance compared with controls. The rises in Isc and transepithelial potential difference induced by glucose (10 mM) were significantly reduced by Cryptosporidium infection (P < .01) compared with controls. In contrast, responses to mucosal glutamine were marginally affected. Electrical parameters of the ileum were not affected by the addition of indomethacin or furosemide, in either control or Cryptosporidium-infected mice. Thus, long-term cryptosporidiosis in immunocompromised animals leads to a reduction in net ion exchanges, decreased paracellular shunting, and impaired Na+-glucose cotransport in the ileum, without prostanoid- or enterotoxin-mediated electrogenic Cl- secretion.
Kapel
N
N
Laboratoire de Biologie Animale et Parasitaire, UFR des Sciences Pharmaceutiques et Biologiques, Université René Descartes, Paris, France.
Huneau
J F
JF
Magne
D
D
Tomé
D
D
Gobert
J G
JG
D000818:Animals; D003457:Cryptosporidiosis / Q000378:metabolism*; D016785:Cryptosporidium parvum; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism*; D007082:Ileum / Q000378:metabolism; D016867:Immunocompromised Host; D007408:Intestinal Absorption; D017136:Ion Transport; D051379:Mice; D016513:Mice, SCID; D012964:Sodium / Q000378:metabolism*
D012964:Sodium; D005947:Glucose