title: Rapid development of affinity matured monoclonal antibodies using RIMMS.
abstract: Affinity matured murine monoclonal antibody producing cell lines can now be rapidly generated using a novel repetitive, multiple site immunization strategy designated RIMMS. RIMMS capitalizes on rapid hypermutation and affinity maturation events which occur in B cell populations localized within secondary lymphatic tissue early in response to antigenic challenges. A murine myeloma cell line, P3XBcl-2-13, stably transfected with Bcl-2, enhances the outgrowth of hybridomas following somatic fusion with immune lymphocytes isolated from pooled peripheral lymph nodes (PLN) 8-14 days after the initial immunization. Immunizations somatic fusion, screening and isolation of affinity matured IgG secreting monoclonal antibody cell lines occur within a one month time period. By using RIMMS, we have been able to expedite the isolation of affinity matured monoclonal antibodies to numerous antigens, including a drug hapten.
authors.affiliation: Department of Molecular Sciences, Glaxo Wellcome, Research Triangle Park, NC 27709, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Kilpatrick
authors.forename: S A
authors.initials: SA
authors.lastname: Wring
authors.forename: D H
authors.initials: DH
authors.lastname: Walker
authors.forename: M D
authors.initials: MD
authors.lastname: Macklin
authors.forename: J A
authors.initials: JA
authors.lastname: Payne
authors.forename: J L
authors.initials: JL
authors.lastname: Su
authors.forename: B R
authors.initials: BR
authors.lastname: Champion
authors.forename: B
authors.initials: B
authors.lastname: Caterson
authors.forename: G D
authors.initials: GD
authors.lastname: McIntyre
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D019254:Genes, bcl-2 / Q000235:genetics; D006241:Haptens / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007114:Immunization; D051379:Mice; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens