Serum protein determination by high-performance gel-permeation chromatography.
A general high-performance gel-permeation chromatography (HPGPC) method was developed to determine protein in human serum with improved sensitivity and speed. The optimum UV wavelength for protein detection was found to be 210 nm, by comparing the protein values obtained by varying the UV wavelength of the HPLC detection system with the protein values obtained from spectrophotometric protein assays, i.e., the bicinchoninic acid (BCA) method and the biuret method. The analysis time was less than 1 min. Since this HPGPC serum protein assay method is simple and rapid, it is expected to be particularly well adapted for use in clinical laboratories.
Hayakawa
K
K
Laboratory of Metabolism, National Children's Medical Research Center, Tokyo, Japan.
Masuko
M
M
Mineta
M
M
Yoshikawa
K
K
Yamauchi
K
K
Hirano
M
M
Katsumata
N
N
Tanaka
T
T
D000293:Adolescent; D001738:Biuret Reaction; D001798:Blood Proteins / Q000032:analysis*; D002624:Chemistry, Clinical / Q000379:methods; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D008297:Male; D011804:Quinolines / Q000737:chemistry; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D001798:Blood Proteins; D007202:Indicators and Reagents; D011804:Quinolines; D011933:Reagent Kits, Diagnostic; C047117:bicinchoninic acid