Soluble Fcgamma receptor, Fc gammaRIIa2, is present in two forms in human serum and is increased in patients: with stage C chronic lymphocytic leukemia.
Human Fcgamma receptor type II (FcgammaRII/CD32) can be produced in a soluble form, termed FcgammaRIIa2, which contains the extra- and intracellular regions of the receptor, due to an alternative splicing of the transmembrane domain-encoding exon. We show that human sera contain two forms of FcgammaRIIa2. A full-length secreted protein, which has a 32 kDa backbone, can be detected only in some sera while all sera contain a C-terminal truncated form, lacking part of the intracytoplasmic tail, and exhibiting a 24 kDa protein backbone. The 24 kDa form is significantly increased in sera from stage C patients with B chronic lymphocytic leukemia, compared to healthy donors, stage A and B CLL patients, or CLL patients in complete remission.
Astier
A
A
Unité INSERM 255, Institut Curie, Paris, France.
Merle-Beral
H
H
de la Salle
H
H
Moncuit
J
J
Cazenave
J P
JP
Fridman
W H
WH
Hanau
D
D
Teillaud
J L
JL
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000097:blood*; D016466:CHO Cells / Q000378:metabolism; D006224:Cricetinae; D006801:Humans; D007536:Isomerism; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood* / Q000473:pathology; D009367:Neoplasm Staging; D017452:Receptors, IgG / Q000096:biosynthesis / Q000097:blood*; D012995:Solubility
D015703:Antigens, CD; C080568:Fc gamma receptor IIA; D017452:Receptors, IgG