Effects of oxidative stress on glycerolipid acyl turnover in rat hepatocytes.
Lipid peroxidation was induced in freshly isolated rat hepatocytes by incubation in the presence of Fe3+, resulting in accumulation of thiobarbituric acid reactive substances. Analysis of lipid classes revealed that the levels and fatty acid compositions of the two major phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), remained unchanged but the levels of triacylglycerols (TAG) were significantly reduced, and some of their polyunsaturated fatty acids were selectively lost as the result of oxidant treatment. Acyl turnover in PC and PE as determined by 18O incorporation from H2 (18)O-containing media remained largely unchanged during oxidant treatment, while some increased turnover of the saturated fatty acids in TAG was observed. We hypothesize that constitutive recycling of membrane phospholipids rather than selective in situ repair eliminates peroxidized species of PC and PE. TAG could serve as an expendable fatty acid reserve, providing a limited but very dynamic pool of polyunsaturated fatty acids for the resynthesis of phospholipids.
Girón-Calle
J
J
Hormel Institute, University of Minnesota, Austin 55912, USA.
Schmid
P C
PC
Schmid
H H
HH
D000818:Animals; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism*; D007700:Kinetics; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D018384:Oxidative Stress; D010103:Oxygen Isotopes; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005290:Ferric Compounds; D005994:Glycerophosphates; D008054:Lipid Peroxides; D008563:Membrane Lipids; D010103:Oxygen Isotopes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides