title: Cleft palate in mice with a targeted mutation in the gamma-aminobutyric acid-producing enzyme glutamic acid decarboxylase 67.
abstract: The functions of neurotransmitters in fetal development are poorly understood. Genetic observations have suggested a role for the inhibitory amino acid neurotransmitter gamma-aminobutyric acid (GABA) in the normal development of the mouse palate. Mice homozygous for mutations in the beta-3 GABAA receptor subunit develop a cleft secondary palate. GABA, the ligand for this receptor, is synthesized by the enzyme glutamic acid decarboxylase. We have disrupted one of the two mouse Gad genes by gene targeting and also find defects in the formation of the palate. The striking similarity in phenotype between the receptor and ligand mutations clearly demonstrates a role for GABA signaling in normal palate development.
authors.affiliation: Howard Hughes Medical Institute, Department of Human Genetics, University of Utah, School of Medicine, Salt Lake City, UT 84112, USA.
authors.forename: B G
authors.initials: BG
authors.lastname: Condie
authors.forename: G
authors.initials: G
authors.lastname: Bain
authors.forename: D I
authors.initials: DI
authors.lastname: Gottlieb
authors.forename: M R
authors.initials: MR
authors.lastname: Capecchi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002972:Cleft Palate / Q000196:embryology / Q000235:genetics*; D003433:Crosses, Genetic; D005260:Female; D005968:Glutamate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D011247:Pregnancy; D011963:Receptors, GABA-A / Q000235:genetics; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
chemical_list: D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase