title: Purification and characterization of the selenate reductase from Thauera selenatis.
abstract: Thauera selenatis is one of two isolated bacterial species that can obtain energy by respiring anaerobically with selenate as the terminal electron acceptor. The reduction of selenate to selenite is catalyzed by a selenate reductase, previously shown to be located in the periplasmic space of the cell. This study describes the purification of the enzyme from T. selenatis grown anaerobically with selenate. The enzyme is a trimeric alphabetagamma complex with an apparent Mr of 180,000. The alpha, beta, and gamma subunits are 96 kDa, 40 kDa, and 23 kDa, respectively, in size. The selenate reductase contains molybdenum, iron, and acid-labile sulfur as prosthetic group constituents. UV-visible absorption spectroscopy also revealed the presence of one cytochrome b per alphabetagamma complex. The Km for selenate was determined to be 16 microM, and the Vmax was 40 micromol/min/mg of protein. The enzyme is specific for the reduction of selenate; nitrate, nitrite, chlorate, and sulfate were not reduced at detectable rates. These studies constitute the first description of a selenate reductase, which represents a new class of enzymes. The significance of this enzyme in relation to cell growth and energy generation is discussed.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Los Angeles, California 90095-1489, USA.
authors.forename: I
authors.initials: I
authors.lastname: Schröder
authors.forename: S
authors.initials: S
authors.lastname: Rech
authors.forename: T
authors.initials: T
authors.lastname: Krafft
authors.forename: J M
authors.initials: JM
authors.lastname: Macy
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000201:enzymology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D010088:Oxidoreductases; C078108:selenate reductase