Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures.
A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells; a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.
Poulsen
L K
LK
Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
Dalton
H M
HM
Angles
M L
ML
Marshall
K C
KC
Molin
S
S
Goodman
A E
AE
D001419:Bacteria / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D017404:In Situ Hybridization, Fluorescence; D009016:Moraxella / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D015345:Oligonucleotide Probes; D001616:beta-Galactosidase / Q000378:metabolism
D015345:Oligonucleotide Probes; D001616:beta-Galactosidase