Anti-steroidogenic activity of the petroleum ether extract and fraction 5 (fatty acids) of carrot (Daucus carota L.) seeds in mouse ovary.
The petroleum ether extract and fraction 5 (fatty acids) of carrot seeds arrested the normal estrus cycle of adult mouse and reduced the weight of ovaries significantly. The cholesterol and ascorbic acid content in ovaries were significantly elevated due to the treatment with extract and fraction 5 (fatty acids) of carrot seeds. The significant inhibition of delta 5,3-beta-hydroxy steroid dehydrogenase and glucose-6-phosphate dehydrogenase, the two key enzymes involved in ovarian steroidogenesis, were also observed in mouse ovaries after 15 days of treatment. Results of this study revealed that the fraction 5 (fatty acids) present in carrot seeds acts as an antisteroidogenic agent.
Majumder
P K
PK
Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India. kpradip@aiims.ernet.in
Dasgupta
S
S
Mukhopadhaya
R K
RK
Mazumdar
U K
UK
Gupta
M
M
D000473:Alkanes / Q000494:pharmacology*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D005591:Chemical Fractionation; D002784:Cholesterol / Q000378:metabolism; D018552:Daucus carota / Q000378:metabolism*; D004971:Estrus / Q000187:drug effects; D005227:Fatty Acids / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors*; D007535:Isomerases / Q000037:antagonists & inhibitors*; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010578:Petroleum; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds
D000473:Alkanes; D005227:Fatty Acids; D010578:Petroleum; D010936:Plant Extracts; D002784:Cholesterol; D005954:Glucosephosphate Dehydrogenase; D007535:Isomerases; C004544:naphtha; D001205:Ascorbic Acid