Cerebellar granule cell membranes and glutamate mediates transactivation of glutamine synthetase promoter.
Potential region of glutamine synthetase promoter driving astrocyte-specific transactivation, mediated by cerebellar granule cell membrane and glutamate has been identified by deletion analysis of promoter and transient transfection. The promoter region from -420 to -765 was found to be potentially important for this transactivation. These results provided further evidence for importance of neuronal-glial and glutamate-glial interactions in regulation of glial gene expression.
Kedar
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V
Laboratory of Molecular Biology, National Institute of Neurological Disorders and Strokes, National Institute of Health, Bethesda, MD 20892, USA. vishramK@qimr.edu.au
Purohit
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H
Freeze
E
E
Hempel
F
F
D001253:Astrocytes / Q000166:cytology / Q000201:enzymology; D002531:Cerebellum / Q000166:cytology* / Q000201:enzymology; D003594:Cytoplasmic Granules / Q000502:physiology*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D018698:Glutamic Acid / Q000502:physiology*; D006801:Humans; D007425:Intracellular Membranes / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D009474:Neurons / Q000166:cytology / Q000201:enzymology; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D018698:Glutamic Acid; D005974:Glutamate-Ammonia Ligase