Endothelial cell adhesion molecule expression and lymphocyte adhesion to endothelial cells: effect of nitric oxide.
Interactions between circulating leukocytes and vascular endothelial cells are of fundamental importance in controlling normal recirculation and migration of cells into sites of inflammation. Nitric oxide (NO), which is synthesized by vascular endothelial cells, has been reported to decrease the binding of platelets, monocytes, macrophages, and neutrophils to endothelial cells. Using NO donors and inhibitors of the enzyme NO synthase, we found no evidence that physiologically relevant levels of NO alter adhesion of purified lymphocytes to an endothelial cell line derived from human umbilical vein endothelial cells (SGHEC-7). In addition, NO donors did not alter the cell surface expression of VCAM-1, ICAM-1, or E-selectin on SGHEC-7 cells.
Cartwright
J E
JE
Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, London, United Kingdom.
Whitley
G S
GS
Johnstone
A P
AP
D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000737:chemistry / Q000166:cytology*; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology*; D009569:Nitric Oxide / Q000502:physiology*; D009589:Nitrogen Oxides; D005996:Nitroglycerin / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D009603:Nitroso Compounds / Q000494:pharmacology; D026422:S-Nitrosoglutathione; D013096:Spermine / Q000031:analogs & derivatives / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins; D019323:omega-N-Methylarginine / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D009589:Nitrogen Oxides; D009603:Nitroso Compounds; D014409:Tumor Necrosis Factor-alpha; C091861:spermine nitric oxide complex; D009599:Nitroprusside; D019323:omega-N-Methylarginine; D013096:Spermine; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D013482:Superoxide Dismutase; D005996:Nitroglycerin; D005978:Glutathione