[Use of polymerase chain reaction for identifying helminth DNA from the species Trichinella, Fasciola, Echinococcus, Nematodirus, Taenia].
We have used the polymerase chain reaction (PCR) and random amplified polymorphic DNA (RAPD) method to identify DNA polymorphism in three classes of helminths, Cestoda, Nematoda and Trematoda. In this study, RAPD markers have been used for a description of the genetic variability between the families, genera, species and isolates of helminths. We optimized the conditions of the RAPD analysis and revealed the genetic variability of helminths belonging to different taxonomic groups. These results show that the RAPD-PCR method can be considered as a suitable technic for a phylogenetic study of helminths. The problem of an age variability and most specificity of RAPDs markers is discussed.
Romanova
E A
EA
Semenova
S K
SK
Benediktov
I I
II
Ryskov
A P
AP
D000818:Animals; D018509:DNA, Helminth / Q000235:genetics*; D006376:Helminths / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity
D018509:DNA, Helminth