Cloning and sequence analysis of two laccase complementary DNAs from the ligninolytic basidiomycete Trametes versicolor.
Laccases are oxidoreductase enzymes involved in the oxidation of various phenolic compounds. They may play a role in the biodegradation of lignin and in the dechlorination of chlorophenols. The cDNAs encoding laccase LccI and a putative laccase LccIV and the gene for LccI from the white-rot basidiomycete Trametes versicolor were cloned, sequenced and characterized. The genomic DNA of lccI consists of 2128 bp, with the coding region interrupted by 10 introns; the cDNA consists of a 1560 bp open reading frame (ORF). The cDNA of the putative lccIV gene consists of a 1581 bp ORF, with a 794 bp 5' untranslated region. The size of the major transcript for both lccI and lccIV is approximately 2.3 kb. Transcription of lccIV was induced by 2,5-dimethylaniline, whereas the opposite effect was observed for lccI. Laccases I and IV contain highly conserved histidinyl and cysteinyl residues, believed to be involved in binding copper, and share extensive sequence similarity with other laccases produced by both ligninolytic and non-ligninolytic fungi.
Ong
E
E
Biotechnology Laboratory, University of British Columbia, Vancouver, Canada. eong@ljcrf.edu
Pollock
W B
WB
Smith
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary; D004271:DNA, Fungal; D005816:Genetic Complementation Test; D042845:Laccase; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004271:DNA, Fungal; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase