A T-cell model for the biological role of selenium-dependent glutathione peroxidase.
The cytosolic form of selenium-dependent glutathione peroxidase detoxifies both hydrogen and lipid peroxides and therefore represents a major component of the cellular anti-oxidant defenses. In order to study the biological role of this enzyme, we generated an expression construct in a retroviral vector, which when introduced into immortalized human T-cells, resulted in significant increases in the activity of this important enzyme. This effect is stable over extended maintenance in culture. The anti-oxidant defenses in these same cells are also shown to be attenuated by chemically reducing cellular glutathione levels. Collectively, the ability to both increase and decrease the anti-oxidant defenses in human T cells results in a useful model system for the study of oxidative stress and signaling in this cell type.
Diamond
A M
AM
Department of Radiation and Cellular Oncology, University of Chicago, IL 60637 USA.
Kataoka
Y
Y
Murray
J
J
Duan
C
C
Folks
T M
TM
Sandstrom
P A
PA
D002460:Cell Line; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D018384:Oxidative Stress; D012643:Selenium / Q000378:metabolism*; D013601:T-Lymphocytes / Q000201:enzymology*
D005979:Glutathione Peroxidase; D012643:Selenium