title: Minor constituents of photosystem II core complexes: possible regulators of photosystem II protein kinase.
abstract: We recently identified a 58-kDa protein kinase, PS II-PK, closely associated in substoichiometric abundance with a core complex of photosystem II and capable of phosphorylating both the photosystem and its associated light-harvesting proteins. Oxidizing species, produced during aerobic illumination, inhibited the kinase, and the irreversibility of this process is now demonstrated. Other substoichiometric protein constituents of the core preparation were identified as probably originating in the grana margins. These include the cytochrome b6/f complex, the apocytochrome f precursor, and membrane-bound ferredoxin:NADP+ oxidoreductase. PS II-PK was successfully resolved from photosystem II cores and shown to be active in the absence of cytochrome complex.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors.forename: H L
authors.initials: HL
authors.lastname: Race
authors.forename: G
authors.initials: G
authors.lastname: Hind
mesh_terms: D002451:Cell Compartmentation; D003573:Cytochrome b Group / Q000302:isolation & purification; D045346:Cytochrome b6f Complex; D003580:Cytochromes / Q000302:isolation & purification; D045348:Cytochromes f; D008027:Light; D010766:Phosphorylation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D045332:Photosystem II Protein Complex; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism
chemical_list: D003573:Cytochrome b Group; D003580:Cytochromes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D047428:Protein Kinase Inhibitors; D045346:Cytochrome b6f Complex; D045348:Cytochromes f; D011494:Protein Kinases