Identification of the transcriptional repression domain of nuclear factor 1-A.
We previously showed that nuclear factor 1-A (NF1-A) binds to the silencer elements in the glutathione transferase P (GST-P) gene, and the carboxy terminal region of NF1-A represses the transcription activity of human metallothionein IIA (hMTIIA) promoter. In this study, we identified a repression region which is divided into two 100 amino acid domains (RD1 and RD2). RD1 increased the repression activity of RD2 to the hMTIIA promoter activity. The NF1-A repression domain inhibited the promoter activities of not only the hMTIIA gene but also those of the GST-P and CCAAT/enhancer binding protein delta genes. RD1 and RD2 had abundant serine and glycine residues, and proline and serine residues, respectively. Whereas some repression domains identified previously are enriched with alanine, proline, or serine, and are associated with the general transcription factors, the NF1-A repression domains did not interact with transcription factor IIB, TATA-binding protein (TBP), or TBP-associated factors in vitro.
Osada
S
S
Laboratory of Environmental Biochemistry, School of Pharmaceutical Sciences, Osaka University, Suita, Japan.
Ikeda
T
T
Xu
M
M
Nishihara
T
T
Imagawa
M
M
D022762:CCAAT-Enhancer-Binding Proteins; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D005982:Glutathione Transferase / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D051839:NFI Transcription Factors; D009687:Nuclear Proteins / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics*; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D005982:Glutathione Transferase