Pertactin antigens extracted from Bordetella pertussis and Bordetella bronchiseptica differ in the isoelectric point.
Pertactin, which is a membrane-associated antigen of Bordetella pertussis and which is present in many acellular vaccines against whooping cough, has been reported to be similar to the homologous protein in Bordetella bronchiseptica. By running parallel experiments using proteins derived from the two species, we show that the isoelectric point of pertactin from B. pertussis is lower than reported and clearly distinguishable from the homologous protein of B. bronchiseptica.
Pagliaccia
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IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina 1, 53100 Siena, Italy.
Manetti
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Rappuoli
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D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D016950:Bordetella bronchiseptica / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D007526:Isoelectric Point; D013045:Species Specificity; D010566:Virulence Factors, Bordetella
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D010566:Virulence Factors, Bordetella; C068929:pertactin