Metabolic adaptation to fat-supplemented diet by the thoroughbred horse.
Following 10 weeks of fat supplementation a group of aerobically trained thoroughbred horses exhibited a significant decrease in postprandial plasma triacylglycerol concentration. This decrease was associated with a mean 50% increase in plasma total lipase activity following pentosan polysulfate administration and an increase in postprandial plasma cholesterol concentration. A significant increase in the activity of muscle citrate synthase (EC 4.1.3.7), expressed as a ratio to the total fractional area occupied by type I and type IIa muscle fibres, was also observed. No significant change in the concentration of resting muscle glycogen or triacylglycerol occurred as a result of fat supplementation. These results suggest that there was improved management of the fat load and that the triacylglycerol-clearing capacity of the horses was increased as a result of fat supplementation. It is suggested that the increase in plasma total lipase activity following pentosan polysulfate administration may have reflected an increase in muscle lipoprotein lipase (EC 3.1.1.34) activity, which would increase the capacity of muscle for free fatty acid uptake from circulating triacylglycerol-rich plasma lipoproteins. Fat supplementation may also enhance the oxidative capacity of muscle, as suggested by the significant increase in muscle citrate synthase and the trend towards an increase in beta-hydroxyacyl CoA dehydrogenase (EC 1.1.1.35) following 10 weeks of fat supplementation.
Orme
C E
CE
Department of Physiology, Animal Health Trust, Newmarket.
Harris
R C
RC
Marlin
D J
DJ
Hurley
J
J
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000222:Adaptation, Physiological; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002784:Cholesterol / Q000097:blood; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D006736:Horses / Q000378:metabolism*; D008049:Lipase / Q000097:blood; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D010426:Pentosan Sulfuric Polyester / Q000008:administration & dosage; D014280:Triglycerides / Q000097:blood
D004041:Dietary Fats; D014280:Triglycerides; D010426:Pentosan Sulfuric Polyester; D002784:Cholesterol; D015094:3-Hydroxyacyl CoA Dehydrogenases; D002950:Citrate (si)-Synthase; D008049:Lipase; D008071:Lipoprotein Lipase