title: The role of oestrogen in follicular development.
abstract: Most of our knowledge of ovarian physiology is based upon studies that have demonstrated functional oestrogen receptors in the ovaries of lower animal species. The presence of oestrogen receptors in primate granulosa cells has been questioned by some investigators. However, we have found oestrogen receptor messenger RNA in human granulosa cells by reverse transcriptase-PCR assay. Furthermore, using immortalized granulosa cell lines transfected with a plasmid containing an oestrogen response element, a functional oestrogen receptor was confirmed. These experiments strongly support the hypothesis that human granulosa cells express biologically active oestrogen receptor.
authors.affiliation: Department of Obstetrics and Gynecology, University of Colorado Health Sciences Center, Denver, USA.
authors.forename: B S
authors.initials: BS
authors.lastname: Hurst
authors.forename: K K
authors.initials: KK
authors.lastname: Leslie
mesh_terms: D000818:Animals; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000502:physiology*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011323:Primates; D012333:RNA, Messenger / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014162:Transfection / Q000379:methods
chemical_list: D012333:RNA, Messenger; D011960:Receptors, Estrogen