Dynamic features of human immunodeficiency virus type 1 (HIV-1) viremia: kinetics of cell-free HIV-1 RNA after therapeutic plasma exchange.
To gain insight into the variables that influence the dynamics of human immunodeficiency virus type 1 (HIV-1) viremia levels, HIV-1 RNA molecules were quantified in plasma from an infected patient undergoing therapeutic plasma exchange (TPEx). After each TPEx procedure (2000 mL of fluid exchanged per session), HIV-1 genome molecule levels dropped to 58%-63% of the basal level but rapidly reverted to pre-TPEx values (doubling time = 3.50-4.04 h). Of interest, mobilization of extravascular cell-free virions (on average, 5.15 x 10(4) viral genome molecules/h) had already occurred during TPEx. After three daily TPEx procedures, HIV-1 viremia rebounded to basal values, while HIV-1 proviruses and viral transcripts in peripheral blood lymphocytes constantly tested at stable levels. Overall, this study extends previous analyses of the rate of HIV-1 turnover, using an alternative approach to the use of antiretroviral drugs, and it provides, albeit indirectly, insights into the amount and dynamic features of extravascular cell-free virus.
Bagnarelli
P
P
Istituto di Microbiologia, Università di Ancona, Italy.
Candela
M
M
Valenza
A
A
Manzin
A
A
Solforosi
L
L
Mazzola
F
F
Butini
L
L
Montroni
M
M
Gabrielli
A
A
Varaldo
P E
PE
Clementi
M
M
D002474:Cell-Free System; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D006526:Hepatitis C / Q000150:complications; D006801:Humans; D007700:Kinetics; D008875:Middle Aged; D010523:Peripheral Nervous System Diseases / Q000150:complications / Q000628:therapy; D010951:Plasma Exchange; D012367:RNA, Viral / Q000097:blood*; D014766:Viremia / Q000150:complications / Q000821:virology*
D012367:RNA, Viral