Astrocyte-conditioned medium induces blood-brain barrier properties in endothelial cells.
1. We evaluated the induction of endothelial barrier functions by the type 1 astrocyte-derived supernatant in culture using horseradish peroxidase (HRP) as a parameter. 2. A gel filtration fraction (molecular weight 16-20 kDa) of type 1 astrocyte-derived supernatant reduced plasmalemmal vesicles and induced tight junctions in endothelial cells. 3. In addition, this fraction promoted the uptake of [3H]-glucosamine by endothelial cells. This cell activity disappeared after heating at 95 degrees C for 10 min and was sensitive to trypsin. 4. These results suggest that a type 1 astrocyte-derived protein factor(s) promotes proteoglycan synthesis with a resultant increase in charge selectivity in endothelial cells and plays an important role in the induction of blood-brain barrier functions.
Yamagata
K
K
Biomedical Division, Sumitomo Metal Industries Ltd, Tokyo, Japan.
Tagami
M
M
Nara
Y
Y
Mitani
M
M
Kubota
A
A
Fujino
H
H
Numano
F
F
Kato
T
T
Yamori
Y
Y
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005944:Glucosamine / Q000378:metabolism; D006735:Horseradish Peroxidase; D008854:Microscopy, Electron; D008970:Molecular Weight; D051381:Rats
D017077:Culture Media, Conditioned; D006735:Horseradish Peroxidase; D005944:Glucosamine