title: Isolation and pathogenicity of Australian strains of Eimeria praecox and Eimeria mitis.
abstract: OBJECTIVE
  To determine the presence of E praecox and E mitis in Australia, to isolate representative strains of these species from chickens and determine their pathogenicity.
  
  
  METHODS
  Morphological, physiological and cross protection studies were undertaken to confirm the identity of Australian isolates of E praecox and E mitis.
  
  
  METHODS
  Oocysts were isolated from a backyard flock at Jimboomba, southeastern Queensland and numbers of E praecox and E mitis enriched by passage in chickens immune to five other species of poultry Eimeria. Oocysts of mean conformation and size of the two species were purified by single oocyst passage. Two isolates that closely matched recorded parameters for E praecox and E mitis were selected and designated JP and JM respectively. The cross protection between the isolates and E acervulina was determined by infection and challenge experiments. The virulence of the two isolates was determined by comparing weight gains of groups of birds inoculated with JP isolate or JM isolate with untreated groups.
  
  
  RESULTS
  Isolates JP and JM most closely matched recorded parameters of E praecox and E mitis respectively. Groups of chickens, previously infected with JP and JM isolates, showed no significant protection against infection with E acervulina. In a separate trial, groups of susceptible chickens inoculated with 10(5) oocysts of JP and JM isolates showed significantly reduced weight gains compared with untreated controls.
  
  
  CONCLUSIONS
  Isolates JP and JM are E praecox and E mitis respectively, confirming the presence of these species in Australia. These isolates were found capable of causing significant reductions in weight gains in susceptible chickens.
authors.affiliation: Department of Primary Industries, Animal Research Institute, Moorooka, Queensland.
authors.forename: W K
authors.initials: WK
authors.lastname: Jorgensen
authors.forename: N P
authors.initials: NP
authors.lastname: Stewart
authors.forename: P J
authors.initials: PJ
authors.lastname: Jeston
authors.forename: J B
authors.initials: JB
authors.lastname: Molloy
authors.forename: G W
authors.initials: GW
authors.lastname: Blight
authors.forename: R J
authors.initials: RJ
authors.lastname: Dalgliesh
mesh_terms: D000818:Animals; D001315:Australia / Q000453:epidemiology; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004198:Disease Susceptibility; D004539:Eimeria / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology*; D015430:Weight Gain / Q000502:physiology
chemical_list: D016054:DNA, Protozoan