title: Effects of photofrin II and light on cellular adenine nucleotides and their modulation.
abstract: Effects of photofrin II (PII) and light on the intra cellular nucleotide levels have been investigated using BHK-21 cell line. Results indicate that lower concentrations of photofrin II in dark increases ATP levels in a non linear manner, however, there has been no change in energy charge and levels of other nucleotides. Photoirradiation of PII-treated cells leads to a significant reduction in ATP levels and energy charge along with an increase in ATP breakdown products like ADP and AMP. The phosphorylation potential [ATP]/[ADP][Pi] also reduces upon photoirradiation of PII treated cells. Incubation conditions like pH of the medium and temperature modulate the cellular responses to a great extent.
authors.affiliation: Department of Biocybernetics, Institute of Nuclear Medicine & Allied Sciences, Lucknow Marg, Delhi, India.
authors.forename: F
authors.initials: F
authors.lastname: Khanum
authors.forename: V
authors.initials: V
authors.lastname: Jain
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism* / Q000528:radiation effects; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D017323:Dihematoporphyrin Ether / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D008027:Light; D010778:Photochemotherapy; D013696:Temperature
chemical_list: D000227:Adenine Nucleotides; D017323:Dihematoporphyrin Ether