title: Enzymatic synthesis of oligosaccharides containing Gal beta-->4Gal disaccharide at the non-reducing end using beta-galactanase from Penicillium citrinum.
abstract: The transglycosylation reaction was done with a beta-galactanase from Penicillium citrinum. The regioselectivity in the transglycosylation reaction was studied using soy bean arabinogalactan as a donor and mono- or disaccharide derivatives containing beta-galactosyl residue as acceptors. We also synthesized oligosaccharides containing Gal beta 1-->4Gal sequence such as Gal beta 1-->4Gal beta1-->4Glc, Gal beta 1-->4Gal beta 1-->3GlcNac, Gal beta 1-->4Gal beta 1-->4GlcNAc, Gal beta 1-->4Gal beta 1-->6GlcNAc, and Gal beta 1-->4Gal beta 1-->3GalNAc for use in the total synthesis of complex sugar chains.
authors.affiliation: Meiji Institute of Health Science, Naruda Odawara, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fujimoto
authors.forename: H
authors.initials: H
authors.lastname: Nakano
authors.forename: M
authors.initials: M
authors.lastname: Isomura
authors.forename: S
authors.initials: S
authors.lastname: Kitahata
authors.forename: K
authors.initials: K
authors.lastname: Ajisaka
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000096:biosynthesis*; D006026:Glycoside Hydrolases; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis*; D010407:Penicillium / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D009844:Oligosaccharides; D006026:Glycoside Hydrolases; C089138:beta-galactanase; D001616:beta-Galactosidase