Pradimicin, a mannose-binding antibiotic, induced carbohydrate-mediated apoptosis in U937 cells.
Pradimicin (PRM), a mannose-binding antifungal antibiotic, recognizes a D-mannoside in the presence of calcium. We demonstrated that BMY-28864, a semi-synthetic analog of PRM, induced apoptosis in U937 cells which had been incubated with 1-deoxymannojirimycin (DMJ). Characteristic morphological changes such as formation of apoptotic bodies and DNA fragmentation were observed in apoptotic cells.
Oki
T
T
Toyama Prefectural University, Biotechnology Research Center, Japan. oki@pu-toyama.ac.jp
Yamazaki
Y
Y
Furumai
T
T
Igarashi
Y
Y
D017485:1-Deoxynojirimycin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000954:Antigens, Surface / Q000096:biosynthesis; D017209:Apoptosis / Q000187:drug effects*; D002241:Carbohydrates / Q000502:physiology*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D000954:Antigens, Surface; D002241:Carbohydrates; D004273:DNA, Neoplasm; D009844:Oligosaccharides; D017485:1-Deoxynojirimycin; D008358:Mannose