[The fractional composition of hydroxylamine preparations and of the lipopolysaccharide of a vaccinal strain of Klebsiella pneumoniae].
The fractional composition of the vaccine preparation obtained by the hydroxylamine treatment of K. pneumoniae 204 cells was studied with the use of gel chromatography in sepharose CL-6B. The preparation was shown to form 2 peaks, only the first high molecular peak being serologically active. The method for the treatment of the culture, obtained directly from a fermenter and stored in a frozen state before treatment, was proposed. In the technology of the manufacture of Klebsiella vaccine preparation the possibility of replacing dialysis with gel chromatography was shown.
Miriasova
L V
LV
Efimtseva
O V
OV
Karabak
I V
IV
Basnak'ian
I A
IA
Egorova
N B
NB
D000818:Animals; D001428:Bacterial Vaccines / Q000737:chemistry* / Q000276:immunology; D002850:Chromatography, Gel; D003470:Culture Media; D005285:Fermentation; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000737:chemistry* / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000254:growth & development / Q000276:immunology*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008970:Molecular Weight
D001428:Bacterial Vaccines; D003470:Culture Media; D006898:Hydroxylamines; D008070:Lipopolysaccharides; D019811:Hydroxylamine