Investigation of aberrant translational control of c-myc in cell lines derived from patients with multiple myeloma.
In cell lines derived from patients with multiple myeloma (MM) we have found an elevation in the amount of the c-myc protein which is not accompanied by an increase in the level of mRNA or a change in the half-life of the protein. There is a 3.4 fold enhancement in the degree of association of the c-myc message with polysomes. This is not accompanied by an alteration in polysome size or a change in the transit time of the c-myc mRNA on the polysomes thus suggesting that there is in increase in the degree of mobilisation of the c-myc message. Sequencing of the c-myc 5'UTR has revealed the presence of a mutation in all the MM cell lines studied and we demonstrate that this mutation causes altered binding of cellular proteins to this RNA species.
Willis
A E
AE
Department of Biochemistry, University of Leicester, United Kingdom.
Paulin
F E
FE
West
M J
MJ
Whitney
R L
RL
D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics; D016259:Genes, myc; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics*; D017354:Point Mutation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm