title: Gas chromatographic-mass spectrometric identification of main metabolites of stanozolol in cattle after oral and subcutaneous administration.
abstract: An analytical method has been developed in order to control the illegal use of stanozolol as growth promoter in livestock. The procedure was based on enzymatic hydrolysis, purification on a Clean Screen DAU column and derivatization with heptafluorobutyric anhydride prior to GC-MS analysis. This method allowed us to study the metabolism of stanozolol in cattle after oral and subcutaneous administrations. Urinary metabolites were identified by mass spectrometry. Stanozolol and 16-hydroxystanozolol were detected after oral administration, while 16-hydroxystanozolol and 4,16-dihydroxystanozolol were found after subcutaneous administration.
authors.affiliation: LDH-LNR, Ecole Nationale Vétérinaire (Ministère de l'Agriculture), Nantes, France.
authors.forename: V
authors.initials: V
authors.lastname: Ferchaud
authors.forename: B
authors.initials: B
authors.lastname: Le Bizec
authors.forename: M P
authors.initials: MP
authors.lastname: Montrade
authors.forename: D
authors.initials: D
authors.lastname: Maume
authors.forename: F
authors.initials: F
authors.lastname: Monteau
authors.forename: F
authors.initials: F
authors.lastname: André
mesh_terms: D000279:Administration, Cutaneous; D000284:Administration, Oral; D045930:Anabolic Agents / Q000008:administration & dosage / Q000652:urine*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D013197:Stanozolol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000652:urine*; D015813:Substance Abuse Detection / Q000662:veterinary*; D019155:Veterinary Drugs / Q000008:administration & dosage / Q000652:urine*
chemical_list: D045930:Anabolic Agents; D019155:Veterinary Drugs; C108320:16-hydroxystanozolol; C108319:4,16-dihydroxystanozolol; D013197:Stanozolol