Yeast bcy1 mutants with stationary phase-specific defects.
Entry into the stationary phase requires the yeast BCY1 gene, which encodes the regulatory subunit of the cAMP-dependent protein kinase (cAPK). New bcy1 mutants, constructed by in vitro mutagenesis of the 3'-region encoding the cAMP-binding domains, were classified as early or late-acting mutants based on viability studies. The late-acting bcy1 mutants accumulated fewer stationary phase-specific Bcy1p isoforms and had decreased cAPK activity. This late-acting class is novel and dies after 7 days in culture, later than two previously reported stationary phase mutants, ubi4 and ard1.
Peck
V M
VM
Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA.
Fuge
E K
EK
Padilla
P A
PA
Gomez
M A
MA
Werner-Washburne
M
M
D000085:Acetates / Q000378:metabolism; D000595:Amino Acid Sequence; D002244:Carbon / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D010697:Eosine I Bluish / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D007527:Isoenzymes; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D000085:Acetates; D007527:Isoenzymes; D010455:Peptides; D011994:Recombinant Proteins; C061479:protein kinase inhibitor peptide; D002244:Carbon; D006003:Glycogen; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D009584:Nitrogen; D010697:Eosine I Bluish