Efficient gene transduction by Epstein-Barr-virus-based vectors coupled with cationic liposome and HVJ-liposome.
We show here a novel non-viral strategy to transduce human cells by using an EBV-based vector system. The EBV-based vectors, the plasmid vectors carrying EBV oriP (origin for plasmid replication) and EBNA (EBV nuclear antigen) 1 gene from EBV genome, were combined with 2 gene delivery systems, i.e., cationic liposome and HVJ-liposome. By both methods, EBV-based vectors could be more efficiently transfected into HeLa cells than non-EBV, conventional plasmid vectors. When human primary fibroblasts were transfected, EBV-based vectors coupled with cationic liposome but HVJ-liposome resulted in successful gene transduction, while human bone marrow cells were transduced with both HVJ-liposome- and cationic liposome-EBV vectors. These results suggest the potential applications of the EBV-based vector system for gene therapy.
Satoh
E
E
Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
Osawa
M
M
Tomiyasu
K
K
Hirai
H
H
Shimazaki
C
C
Oda
Y
Y
Nakagawa
M
M
Kondo
M
M
Kinoshita
S
S
Mazda
O
O
Imanishi
J
J
D001854:Bone Marrow Cells; D002412:Cations; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008081:Liposomes; D018108:Respirovirus / Q000235:genetics*; D014162:Transfection
D002412:Cations; D008081:Liposomes