Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells.
There is growing evidence that T helper cell subsets (TH1 and TH2) can be differentially recruited to promote different types of inflammatory reactions. Murine TH1 but not TH2 cells are recruited through P- and E-selectin into inflamed tissues, where they induce delayed-type hypersensitivity reactions. The human eotaxin-receptor CCR3, originally described on eosinophils and basophils, was also found to be expressed by TH2 cells. An antibody to CCR3 was used to isolate T cells from peripheral blood that give rise to TH2-polarized cell lines and to identify TH2 cells derived from naïve T cells in vitro. Eotaxin stimulated increases in intracellular calcium and chemotaxis of CCR3(+) T cells. The attraction of TH2 cells by eotaxin could represent a key mechanism in allergic reactions, because it promotes the allergen-driven production of interleukin-4 and interleukin-5 necessary to activate basophils and eosinophils.
Sallusto
F
F
Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland. sallusto@bii.ch
Mackay
C R
CR
Lanzavecchia
A
A
D000328:Adult; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002469:Cell Separation; D054413:Chemokine CCL11; D019742:Chemokines, CC; D002634:Chemotaxis, Leukocyte; D002999:Clone Cells; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis; D007377:Interleukin-3 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D054397:Receptors, CCR3; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism* / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
C515456:CCL11 protein, human; C515350:CCR3 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines; D016898:Interferon-alpha; D007377:Interleukin-3; D054397:Receptors, CCR3; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D016212:Transforming Growth Factor beta; D015847:Interleukin-4; D007371:Interferon-gamma; D002118:Calcium