title: Crystal structure of a new RNA-binding domain from the antiterminator protein SacY of Bacillus subtilis.
abstract: SacY belongs to a family of, at present, seven bacterial transcriptional antiterminators. The RNA-binding and antitermination capacity of SacY resides in the 55 amino acids at the N-terminal [SacY(1-55)]. The crystal structure at 2 A resolution shows that SacY(1-55) forms a dimer in the crystal, in accordance with the NMR solution structure. The structure of the monomer is a four-stranded beta-sheet with a simple beta1beta2beta3beta4 topology. One side of the sheet is covered by a long surface loop and the other side forms the dimer interface. The dimer is stabilized by the orthogonal stacking of the two beta-sheets. The crystal structure is in excellent agreement with the NMR solution structure (r.m.s. distance for C alpha coordinates is 1.3 A). The structure of SacY(1-55) reveals a new RNA-binding motif.
authors.affiliation: Centre de Biochimie Structurale, CNRS-U9955, INSERM-U414, Montpellier, France. herman@tome.cbs.univ-montp1.fr
authors.forename: H
authors.initials: H
authors.lastname: van Tilbeurgh
authors.forename: X
authors.initials: X
authors.lastname: Manival
authors.forename: S
authors.initials: S
authors.lastname: Aymerich
authors.forename: J M
authors.initials: JM
authors.lastname: Lhoste
authors.forename: C
authors.initials: C
authors.lastname: Dumas
authors.forename: M
authors.initials: M
authors.lastname: Kochoyan
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D018360:Crystallography, X-Ray; D019281:Dimerization; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C076894:SacY protein, Bacillus subtilis; D014157:Transcription Factors; C064872:antiterminator proteins, Bacteria