High-performance liquid chromatographic analysis of bilirubin and biliverdin from jaundiced broilers.
A sensitive and rapid high-performance liquid chromatographic (HPLC) method was developed and used for the simultaneous determination of bilirubin and biliverdin in pericardial fluid samples collected from broilers at a poultry inspection site. A photodiode array detector distinguishing the bilirubin (UV 450 nm) and biliverdin (365 nm) was used as an analytical detector for HPLC system. An internal-surface reversed-phase silica support column was used, and the mobile phase consisted of acetonitrile: 0.5 M Tris HCl buffer (20:80, pH 7.2). Bilirubin was detected from all of the jaundiced pericardial fluid samples, and a small amount of biliverdin was detected with bilirubin in some samples. These jaundiced broilers had hepatic or bile duct lesions similar to those found in edible animals. From these results, a working definition of jaundiced broilers for poultry inspection sites was suggested: bilirubin is detectable from pericardial fluid and the carcass is in a state of yellow color change.
Mizobe
M
M
Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University, Japan.
Kondo
F
F
Kumamoto
K
K
Kanda
Y
Y
Seguchi
H
H
D000818:Animals; D001663:Bilirubin / Q000032:analysis*; D001664:Biliverdine / Q000032:analysis*; D002645:Chickens; D002779:Cholestasis / Q000097:blood / Q000453:epidemiology / Q000662:veterinary; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004196:Disease Outbreaks / Q000662:veterinary*; D006520:Hepatitis, Animal / Q000175:diagnosis / Q000453:epidemiology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007565:Jaundice / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D008099:Liver / Q000473:pathology*; D010496:Pericardium; D011201:Poultry Diseases
D001664:Biliverdine; D001663:Bilirubin