Differential expression of the growth hormone receptor and its transcript in bovine uterus and placenta.
By reverse transcription-polymerase chain reaction (RT-PCR) the transcript of the growth hormone receptor (GHR) was proved in bovine placentae of different gestational stages. Using non-radioactive in situ hybridization (ISH) the mRNA encoding GHR was localized in the uterine epithelium, glands, vessels and amniochorion of the placenta from the sixth week until the ninth month of gestation. Production of mRNA was low during the first trimester, significantly increased in the second trimester and peaked at the end of the sixth month of pregnancy. No transcript of GHR was found in the endometrium of non-pregnant uteri. The colocalization of the protein using a monoclonal antibody showed the same distribution pattern as the mRNA. Immunoblotting revealed a protein with a molecular weight of 120 kDa in the endometrium of pregnant cows which was not found in non-pregnant uteri. Our results imply that growth hormone is involved in placental metabolism and embryonic development from the early beginning of pregnancy until birth.
Kölle
S
S
Department of Veterinary Anatomy, University of Munich, Germany.
Sinowatz
F
F
Boie
G
G
Lincoln
D
D
Waters
M J
MJ
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D015151:Immunoblotting; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D010920:Placenta / Q000737:chemistry / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase; D011986:Receptors, Somatotropin / Q000032:analysis / Q000235:genetics*; D014599:Uterus / Q000737:chemistry / Q000378:metabolism*
D012333:RNA, Messenger; D011986:Receptors, Somatotropin; D012194:RNA-Directed DNA Polymerase