The effect of ashing on cells: spectromicroscopy of physiological elements.
We analyzed the effects of cold oxygen plasma ashing of neurobiological specimens on different elements with synchrotron spectromicroscopy. Our results demonstrate that while carbon is almost completely removed, phosphorus, calcium, potassium, sulfur, and, to some extent, nitrogen are retained and their relative concentration is enhanced.
De Stasio
G
G
Institut de Physique Appliquée, Ecole Polytechnique Fédérale, Lausanne, Switzerland.
Capozi
M
M
Droubay
T C
TC
Mercanti
D
D
Ciotti
M T
MT
Lorusso
G F
GF
Andres
R
R
Suda
T
T
Perfetti
P
P
Tonner
B P
BP
Margaritondo
G
G
D000818:Animals; D002118:Calcium / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002531:Cerebellum / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D011188:Potassium / Q000737:chemistry; D051381:Rats; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D013455:Sulfur / Q000737:chemistry; D016707:Tissue Fixation / Q000379:methods*
D010758:Phosphorus; D013455:Sulfur; D002244:Carbon; D009584:Nitrogen; D011188:Potassium; D010100:Oxygen; D002118:Calcium