title: SNAP-23 is located in the basolateral plasma membrane of rat pancreatic acinar cells.
abstract: The SNARE hypothesis proposes that specificity of exocytosis is regulated by the appropriate interactions between the vesicle (v-) SNARE and the target membrane (t-) SNAREs. We show here that pancreatic acinar cells express the SNAP-25 t-SNARE homolog SNAP-23, and find that this t-SNARE is most highly concentrated on the basolateral plasma membrane while being expressed below detectable levels in endocrine islets within the same tissue. This is the first localization of SNAP-23 within a polarized tissue and suggests that this t-SNAREs may interact with syntaxin-4 to mediate basolateral secretion.
authors.affiliation: Department of Medicine, University of Toronto, The Toronto Hospital, Ont., Canada.
authors.forename: H Y
authors.initials: HY
authors.lastname: Gaisano
authors.forename: L
authors.initials: L
authors.lastname: Sheu
authors.forename: P P
authors.initials: PP
authors.lastname: Wong
authors.forename: A
authors.initials: A
authors.lastname: Klip
authors.forename: W S
authors.initials: WS
authors.lastname: Trimble
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000648:ultrastructure; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D007425:Intracellular Membranes / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D018613:Microscopy, Confocal; D010179:Pancreas / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002352:Carrier Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C100493:SNAP23 protein, human; C107017:Snap23 protein, mouse