The lipopolysaccharide of Helicobacter mustelae type strain ATCC 43772 expresses the monofucosyl A type 1 histo-blood group epitope.
The lipopolysaccharide of Helicobacter mustelae type strain ATCC 43772 was obtained by phenol-water extraction of bacterial cells. Structural investigations were made on the lipid A free saccharide moiety released from the lipopolysaccharide by mild acetic acid hydrolysis. Nuclear magnetic resonance, gas liquid chromatography-mass spectrometry and fast atom bombardment-mass spectrometry were employed in the characterization of products from chemical manipulations. A monoclonal antibody specific for blood group A reacted strongly with lipopolysaccharide of H. mustelae. Chemical and serological data showed that the outer core region of the lipopolysaccharide from H. mustelae ATCC 43772 expresses the monofucosyl A type 1, alpha-D-GalNAc-(1-->3)-[alpha-L-Fuc-(1-->2]-beta-D-Gal-(1-->3)- beta-D-GlcNAc, blood group determinant, a mimic of animal cell surface glycolipids and glycoproteins.
Monteiro
M A
MA
Canadian Bacterial Diseases Network, Institute for Biological Sciences, National Research Council Canada, Ottawa, Ont., Canada. Mario.Monteiro@nrc.ca
Zheng
P Y
PY
Appelmelk
B J
BJ
Perry
M B
MB
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000032:analysis*; D005289:Ferrets; D005643:Fucose; D005753:Gastric Mucosa / Q000382:microbiology; D005756:Gastritis / Q000382:microbiology; D016998:Helicobacter / Q000737:chemistry* / Q000276:immunology; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data
D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D008070:Lipopolysaccharides; C109615:monofucosyl A type 1; D005643:Fucose