Human growth hormone antagonist (G120R) delivered by a murine yolk sac cell-derived mini-organ decreases the growth rate of mice.
Long-term cultured murine embryonic yolk sac cells that are capable of forming capillary structures when cultured on base membrane proteins (Matrigel) were successfully transfected with a human growth hormone antagonist (G120R) gene. Cells that stably express relatively high levels of G120R were co-implanted s.c. with Matrigel into BALB/c mice. G120R can be detected in the sera of those implanted mice for more than 14 days at levels from 4 ng/ml to 28 ng/ml. The insulin-like growth factor-1 levels in the sera of those implanted mice were significantly affected by the delivered G120R. One of the physiological effects of G120R delivered by this murine embryonic yolk sac cell-derived mini-organ system is to decrease the growth rate of the implanted mice. This gene delivery system can also be used as an alternative to transgenic animals to study protein function in vivo.
Wei
Y
Y
Edison Biotechnology Institute, Molecular and Cellular Biology Program, Ohio University, Athens 45701, USA.
Li
J
J
Chen
W Y
WY
Wagner
T E
TE
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002460:Cell Line; D003094:Collagen / Q000235:genetics / Q000494:pharmacology; D046508:Culture Techniques; D004338:Drug Combinations; D015870:Gene Expression / Q000235:genetics / Q000502:physiology; D018014:Gene Transfer Techniques; D006128:Growth / Q000187:drug effects* / Q000235:genetics; D013006:Growth Hormone / Q000031:analogs & derivatives* / Q000097:blood / Q000235:genetics / Q000502:physiology; D006727:Hormone Antagonists / Q000097:blood; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D007797:Laminin / Q000235:genetics / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011509:Proteoglycans / Q000235:genetics / Q000494:pharmacology; D011994:Recombinant Proteins
D001672:Biocompatible Materials; D004338:Drug Combinations; C093172:G120R protein, human; D006727:Hormone Antagonists; D007797:Laminin; D011509:Proteoglycans; D011994:Recombinant Proteins; C059485:matrigel; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D003094:Collagen