Quantitative analysis of C60 fullerene in blood and tissues by high-performance liquid chromatography with photodiode-array and mass spectrometric detection.
A high-performance liquid chromatographic (HPLC) assay method for C60 fullerene, in blood, liver and spleen using photodiode-array detection or mass spectrometric detection (LC-MS) and C70 fullerene, as the internal standard, is described. The recovery from mouse blood and tissues spiked with micronized C60 exceeds 90%. The method is linear from 0.05 to 200 mg of C60 per liter of blood and from 0.05 to 5.00% of C60 per tissue weight. The limit of detection of the method is 0.1 ng of C60 per injection. This method was applied to mouse blood and tissue samples after intraperitoneal administration of a micronized C60 suspension.
Moussa
F
F
Jeune Equipe 1990, Faculte de Pharmacie, Tours, France.
Pressac
M
M
Genin
E
E
Roux
S
S
Trivin
F
F
Rassat
A
A
Céolin
R
R
Szwarc
H
H
D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D002244:Carbon / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D004350:Drug Residues / Q000032:analysis*; D037741:Fullerenes; D007274:Injections, Intraperitoneal; D016014:Linear Models; D008099:Liver / Q000737:chemistry*; D008297:Male; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms; D013056:Spectrophotometry, Ultraviolet; D013154:Spleen / Q000737:chemistry*
D000998:Antiviral Agents; D037741:Fullerenes; D002244:Carbon; C069837:fullerene C60