title: Adenoviral mediated gene transfer can be accomplished in human myeloid cell lines and is inhibited by all-trans retinoic acid-induced differentiation.
abstract: OBJECTIVE
  Gene transfection could potentially represent a useful therapeutic tool for genetic and neoplastic hematological diseases. After having long been considered poorly able to transfect myeloid cells, adenoviral vectors have recently been demonstrated to be capable of introducing foreign DNA into purified CD34+ cells from human bone marrow or cord blood. In the present study we evaluated the feasibility of adenoviral-mediated gene transfer in two human leukemic cell lines, both at baseline and after differentiation induction by all-trans retinoic acid (ATRA).
  
  
  METHODS
  We used a recombinant adenovirus expressing beta-galactosidase (Ad-RSV-beta-gal) to transfect K562 and HL-60 cell lines. The effects of 10(-6)M ATRA were evaluated after 8 days of exposure. The efficacy of transfection was verified by X-gal staining.
  
  
  RESULTS
  Ad-RSV-beta-gal was able to transfect both the HL-60 and, to a minor extent, the K562 cell lines. The addition of ATRA had no effect on transfection of K562 cells, while a lower percentage of beta-gal-positive cells was detected in HL-60, which underwent differentiation upon ATRA treatment.
  
  
  CONCLUSIONS
  These data suggest that adenoviral-mediated gene transfer could be feasible in myeloid leukemia cell lines and that it is inhibited by ATRA in differentiation-sensitive cells. The latter effect merits further investigation in order to verify whether this represents an ATRA-related or a differentiation-related phenomenon.
authors.affiliation: Institute of Hematology and Medical Oncology Seràgnon, University of Bologna, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Tosi
authors.forename: A
authors.initials: A
authors.lastname: Pellacani
authors.forename: G
authors.initials: G
authors.lastname: Visani
authors.forename: E
authors.initials: E
authors.lastname: Ottaviani
authors.forename: S
authors.initials: S
authors.lastname: Tura
mesh_terms: D000256:Adenoviridae; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D018922:HL-60 Cells; D006801:Humans; D007641:Keratolytic Agents / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D007641:Keratolytic Agents; D014212:Tretinoin