Comparison of biocompatibility of gel-derived bioactive ceramics in macrophage culture conditions.
The behaviour of rat alveolar macrophages cultured in the presence of three new gel-derived ceramic biomaterials (CaO-P2O5-SiO2 system) with slightly different chemical compositions was examined. The abilities of these three materials to stimulate alveolar macrophages were compared. Non-treated and lipopolysaccharide-treated macrophages were used as control. The level of macrophage activation was determined by nitrite and prostaglandin E2 assay and respiratory burst measurement by chemiluminescence. The results of these studies showed different macrophage responses to these three relatively similar stimuli. Two of the studied materials were shown to be potent activators of respiratory burst and prostaglandin E2 secretion without any significant release of nitric oxide. On the contrary, the material characterized as the most surface reactive strongly affected only nitric oxide generation by the cells.
Laçzka-Osyczka
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A
Department of Animal Biochemistry, Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Turyna
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B
Dubin
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A
Laçzka
M
M
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000633:toxicity; D017610:Calcium Compounds; D002478:Cells, Cultured; D002516:Ceramics / Q000494:pharmacology* / Q000633:toxicity; D015232:Dinoprostone / Q000096:biosynthesis; D005782:Gels; D008163:Luminescent Measurements; D008262:Macrophage Activation / Q000187:drug effects; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000502:physiology; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009569:Nitric Oxide / Q000096:biosynthesis; D010087:Oxides; D010758:Phosphorus; D017553:Phosphorus Compounds; D051381:Rats; D016897:Respiratory Burst / Q000187:drug effects; D012822:Silicon Dioxide
D001672:Biocompatible Materials; D017610:Calcium Compounds; D005782:Gels; D010087:Oxides; D017553:Phosphorus Compounds; D010758:Phosphorus; D009569:Nitric Oxide; C012500:phosphorus pentoxide; D012822:Silicon Dioxide; C016538:lime; D015232:Dinoprostone