title: Increased secretion of hepatocyte growth factor by eutopic endometrial stromal cells in women with endometriosis.
abstract: OBJECTIVE
  We evaluated the secretion of hepatocyte growth factor by eutopic endometrial stromal cells in vitro.
  
  
  METHODS
  Eutopic endometrial stromal cells were isolated and the culture supernatants were collected after 48 hours of incubation. The secretion of hepatocyte growth factor was analyzed by ELISA.
  
  
  METHODS
  Department of Obstetrics and Gynecology of Tohoku University Hospital.
  
  
  METHODS
  Specimens of endometrium in the proliferative phase were excised from 11 patients who were undergoing laparoscopy for infertility. Six of the patients had endometriosis and five did not. None of the patients had received hormonal treatment before surgery.
  
  
  RESULTS
  Hepatocyte growth factor secretion was found to be increased significantly in cultured endometrial stromal cells from the infertile patients with endometriosis compared with those without endometriosis.
  
  
  CONCLUSIONS
  The secretion of hepatocyte growth factor was up-regulated in eutopic endometrial stromal cells from patients with endometriosis. An increase in hepatocyte growth factor production may be characteristic of endometriosis and may be involved in the pathophysiology of this disease.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Tohoku University, Sendai, Japan. SUGAWARA@OB-GY.MED.TOHOKU.AC.JP
authors.forename: J
authors.initials: J
authors.lastname: Sugawara
authors.forename: T
authors.initials: T
authors.lastname: Fukaya
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: A
authors.initials: A
authors.lastname: Yajima
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D004715:Endometriosis / Q000209:etiology / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005169:Factor VIII / Q000032:analysis; D005260:Female; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D017228:Hepatocyte Growth Factor; D005169:Factor VIII; D017493:Leukocyte Common Antigens