title: Retinal ischemia induced by the intraluminal suture method in rats.
abstract: In a model of transient focal cerebral ischemia in male Sprague-Dawley rats, which is induced by the intraluminal suture method, the acute effects on the electrical function of the retina were monitored by recording the electroretinogram. The electroretinogram was recorded from halothane-anesthetized rats before, during and after vascular occlusion through the intraluminal suture method for 180 min. During vascular occlusion the amplitude of the a- and b-wave were markedly suppressed. Upon reperfusion the a-wave recovered immediately. During reperfusion up to 48 h the amplitude of the b-wave increased to approximately 50% of the pre-occlusion value did not fully recover. Immunohistochemistry of the retinas revealed that the vascular occlusion induced the expression of glial fibrillary acidic protein in retinal Müller cells. The present data suggest that the intraluminal suture method leads to retinal ischemia.
authors.affiliation: Department of Neurology RWTH Aachen, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Block
authors.forename: C
authors.initials: C
authors.lastname: Grommes
authors.forename: C
authors.initials: C
authors.lastname: Kosinski
authors.forename: W
authors.initials: W
authors.lastname: Schmidt
authors.forename: M
authors.initials: M
authors.lastname: Schwarz
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000503:physiopathology; D017536:Carotid Artery, Common / Q000601:surgery; D004596:Electroretinography; D005123:Eye / Q000098:blood supply*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D002546:Ischemic Attack, Transient / Q000503:physiopathology*; D008026:Ligation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000737:chemistry / Q000503:physiopathology*; D013537:Sutures
chemical_list: D005904:Glial Fibrillary Acidic Protein