title: A single nucleotide is a sufficient 5' untranslated region for translation in an eukaryotic in vitro system.
abstract: The 5' untranslated region of an RNA molecule is thought to play an important role in the regulation of translation. Following a recent report that a single nucleotide is sufficient to act in this role in the unicellular organism Giardia, we show that this is also the case for a mammalian in vitro system. These results also demonstrate that an RNA can initiate translation from a start codon where an ideal translational consensus sequence is impossible.
authors.affiliation: Dept. of Biochemistry, McMaster University, Hamilton, ON, Canada.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hughes
authors.forename: D W
authors.initials: DW
authors.lastname: Andrews
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D018387:Codon, Initiator / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D017930:Genes, Reporter / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011233:Precipitin Tests; D011388:Prolactin / Q000235:genetics; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D012315:RNA Caps / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic; D051028:bcl-2-Associated X Protein
chemical_list: D018387:Codon, Initiator; D011498:Protein Precursors; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012315:RNA Caps; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; C017671:preprolactin; D011388:Prolactin