Expression, purification, and co-crystallization of IRF-I bound to the interferon-beta element PRDI.
Interferon regulatory factor 1 (IRF-1) is an essential factor involved in the regulation of type I interferon (IFN) and IFN-inducible genes. The protein consists of 329 amino acids that are highly conserved from mouse to human. Similar to other transcription factors, the protein is modular in nature with a basic N-terminal region involved in DNA binding and an acidic C-terminal region required for activation. We report here the expression, purification and co-crystallization of the minimal N-terminal region of IRF-1 involved in DNA binding (amino acids 1-113) with a 13 bp DNA fragment from the IFN-beta promoter. The crystals diffract to at least 3.0 A in resolution and belong to space group R3 with unit cell parameters of a = b = 84.8 A, c = 203.7 A.
Escalante
C R
CR
Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA.
Yie
J
J
Thanos
D
D
Aggarwal
A K
AK
D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D004926:Escherichia coli; D050856:Interferon Regulatory Factor-1; D016899:Interferon-beta / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D010206:Papain; D010446:Peptide Fragments / Q000737:chemistry; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry
D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; D010750:Phosphoproteins; D014157:Transcription Factors; D016899:Interferon-beta; D010206:Papain