[The significance of gender incompatibility in donors and recipients and the role of minor histocompatibility antigens in corneal transplantation].
OBJECTIVE
The present study evaluates the ability of single male H-Y antigen to elicit corneal graft rejection or to prime recipient with subsequent induction of second-set corneal graft rejection in mice. Results were compared with the survival rates of orthotopic corneal grafts disparate at multiple minor histocompatibility (H) antigens and with syngeneic grafts.


METHODS
A/Ph male or B10.A female and A/Ph female corneas were grafted onto A/Ph female mice. During the fourth week after grafting all acceptors of H-Y disparate grafts received the second corneal graft from the same donor. All grafts were evaluated three times a week during the period of nine weeks.


RESULTS
Both first and second orthotopic corneal grafts incompatible at H-Y antigen did not display and sign of rejection reaction during the whole observation period and were accepted indefinitely. The rate of rejection reaction of grafts incompatible at multiple minor H antigens was 83%. Thirty-three percent of these grafts underwent rejection irreversibly.


CONCLUSIONS
Single H-Y antigen disparity between corneal graft donor and recipient neither led to rejection of first grafts nor primed recipients as shown by tolerance of 100% of second-set grafts. Experiments demonstrated that incompatibility of sex between donor and recipient does not play role in corneal graft rejection. However, incompatibility at multiple minor H antigens led to the rejection reaction in 83% of first grafts and 33% of these grafts failed.
Hasková
Z
Z
II. ocní klinika 1, LF UK a VFN, Praha.
Filipec
M
M
Holán
V
V
D000818:Animals; D016039:Corneal Transplantation / Q000276:immunology; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D006182:H-Y Antigen; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015778:Minor Histocompatibility Antigens / Q000032:analysis*; D012723:Sex; D014185:Transplantation, Isogeneic
D006182:H-Y Antigen; D015778:Minor Histocompatibility Antigens