Circadian aqueous flow mediated by beta-arrestin induced homologous desensitization.
It was hypothesized that homologous desensitization regulates signal transduction from the beta-adrenergic receptor in the ocular ciliary epithelium to affect the circadian rhythm of aqueous humor secretion. beta-arrestin-1 was cloned from the rabbit ciliary epithelium, and the full length cDNA used as a probe for Northern blot analysis to examine the diurnal expression of beta-arrestin mRNA. Protein expression of beta-arrestin-1 at intervals during the circadian cycle of aqueous secretion showed a decrease in beta-arrestin expression when maximal activation of the beta-adrenergic receptor is known to increase secretion. Diurnal expression of beta-arrestin suggests that homologous desensitization can regulate the circadian rhythm of aqueous flow.
Wan
X L
XL
Yale University School of Medicine, Department of Ophthalmology and Visual Science, New Haven, CT 06520-8061, USA.
Sears
J
J
Chen
S
S
Sears
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001082:Aqueous Humor / Q000502:physiology*; D019393:Arrestin / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015152:Blotting, Northern; D015153:Blotting, Western; D002924:Ciliary Body / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology*; D004848:Epithelium / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005786:Gene Expression Regulation; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D013045:Species Specificity
D019393:Arrestin; D012333:RNA, Messenger