Development and use of a conditional antisense mutagenesis system in mycobacteria.
Expression from a 2.3 kb region upstream of the inducible acetamidase gene from Mycobacterium smegmatis was shown to be upregulated by acetamide. A DNA fragment containing the start of the M. smegmatis hisD gene was cloned in front of the promoter, such that the antisense message was produced. When this construct was induced in vivo, the bacteria became phenotypically histidine auxotrophs; this auxotrophy was restored by histidine supplementation. Auxotrophy was not observed under non-induced conditions. Antisense mutagenesis may be useful for observing the phenotypic inactivation of specific mycobacterial genes, and an inducible system such as that described would allow the study of essential genes.
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Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, UK.
Stoker
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D000081:Acetamides / Q000494:pharmacology; D000581:Amidohydrolases / Q000235:genetics; D016375:Antisense Elements (Genetics); D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D006639:Histidine / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009161:Mycobacterium / Q000187:drug effects / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids; D011401:Promoter Regions, Genetic
D000081:Acetamides; D016375:Antisense Elements (Genetics); D017931:DNA Primers; D004269:DNA, Bacterial; D006639:Histidine; C030686:acetamide; D000581:Amidohydrolases; C021445:acetamidase