title: Anti-apoptotic action of angiotensin fragments to neuronal cells from angiotensinogen knock-out mice.
abstract: The morphological analysis in a congenic line of angiotensinogen knock-out mice (AgKO) revealed the decreased density in granular layer cells of hippocampus and cerebellum, suggesting neuronal cells of AgKO susceptible to apoptotic cell death. This phenomenon was further studied by culture of the hippocampal neurons with decreased concentration of serum. AgKO neuronal cells, which showed apoptosis by lower concentration of the serum within several hours, however, survived much longer in the presence of angiotensin II (AII) and IV (AIV). This anti-apoptotic action was not interfered by AII receptor antagonists, CV11874 and PD123319. These results suggest that the renin-angiotensin system could play a critical role in central nervous system, preventing neuronal cells from apoptosis not only by AII but also AIV.
authors.affiliation: Institute of Basic Medical Sciences, Department of Pharmacology, University of Tsukuba, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kakinuma
authors.forename: H
authors.initials: H
authors.lastname: Hama
authors.forename: F
authors.initials: F
authors.lastname: Sugiyama
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: A
authors.initials: A
authors.lastname: Fukamizu
mesh_terms: D000804:Angiotensin II / Q000031:analogs & derivatives / Q000494:pharmacology*; D000808:Angiotensinogen / Q000502:physiology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002452:Cell Count / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects; D051379:Mice; D018345:Mice, Knockout; D018377:Neurotransmitter Agents / Q000494:pharmacology
chemical_list: D018377:Neurotransmitter Agents; D000808:Angiotensinogen; D000804:Angiotensin II; C012877:angiotensin II, des-Asp(1)-des-Arg(2)-Ile(5)-