The effect of antibodies against cell-surface adhesion molecules (LFA-1 alpha and ICAM-1) on the migration and localization of 99mTc-labeled leukocytes in acute infection.
The deficiency of adhesion molecules on leukocytes could severely impair their ability to migrate and perform effective immunological functions leading to clinical situation such as LAD (leukocyte adhesion deficiency) syndrome. We investigated the effects of blocking anti-LFA-1 alpha and ICAM-1 antibody-treated 99mTc-labeled leukocytes on the migration and localization to the site of E. coli-induced acute infection in CBA/J mice. A significant inhibition of migration and localization of antibody-treated leukocytes to the site of infection was observed, reaffirming the vital role of these adhesion molecules, especially during scintigraphic examination of patients for deep infections or abscess using labeled leukocytes.
Amartey
J K
JK
Radionuclide and Cyclotron Operations Department, King Faisal Specialist Hospital and Research Centre (MBC-03), Riyadh, Kingdom of Saudi Arabia.
Parhar
R S
RS
al-Sedairy
S T
ST
D000208:Acute Disease; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002465:Cell Movement; D004927:Escherichia coli Infections / Q000175:diagnosis*; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D007962:Leukocytes / Q000502:physiology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D013667:Technetium
D000906:Antibodies; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D013667:Technetium