Effect of solution pH on the calcium phosphates formation and ionic diffusion on and through the collagenous matrix.
The effect of pH on the calcification and the permselectivity for the Ca2+ and PO4(3-) ions of a collagenous matrix were studied at pH6.5, 7.0 and 7.4 and at 37 degrees C, using slices of Achilles tendon of a cow, which were cut into disks, in an experimental system where Ca2+ and PO4(3-) ions diffused into the collagen disk from the mutually opposite sides of the disk. The products on and inside the disk were a mixture of octacalcium phosphate (OCP) and apatite regardless of the solution pH. At pH6.5, few crystals grew on the PO4-side of the disk, while many crystals grew on the Ca-side. Crystal growth on the PO4-side was enhanced at higher pH, while that on the Ca-side was suppressed. With an increase in pH, the diffusion of Ca2+ ion through the disk increased, while that of PO4(3-) ion decreased. The mode of crystal growth on the collagen disk was consistent with the mode of ionic diffusion through the matrix. Inside the disk, the influence of pH on the crystal growth was not obvious. It was suggested that the electrochemical property of the collagenous matrix regulated the ionic inflow into the matrix and thereby affected its calcification.
Iijima
M Y
MY
Asahi University, School of Dentistry, Gifu, Japan.
Moriwaki
Y
Y
Yamaguchi
R
R
Kuboki
Y
Y
D000125:Achilles Tendon / Q000378:metabolism; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D003460:Crystallization; D004058:Diffusion; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008855:Microscopy, Electron, Scanning; D012996:Solutions
D002130:Calcium Phosphates; D007477:Ions; D012996:Solutions; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D003094:Collagen; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous