title: High-performance liquid chromatographic analysis of bilirubin and biliverdin from jaundiced broilers.
abstract: A sensitive and rapid high-performance liquid chromatographic (HPLC) method was developed and used for the simultaneous determination of bilirubin and biliverdin in pericardial fluid samples collected from broilers at a poultry inspection site. A photodiode array detector distinguishing the bilirubin (UV 450 nm) and biliverdin (365 nm) was used as an analytical detector for HPLC system. An internal-surface reversed-phase silica support column was used, and the mobile phase consisted of acetonitrile: 0.5 M Tris HCl buffer (20:80, pH 7.2). Bilirubin was detected from all of the jaundiced pericardial fluid samples, and a small amount of biliverdin was detected with bilirubin in some samples. These jaundiced broilers had hepatic or bile duct lesions similar to those found in edible animals. From these results, a working definition of jaundiced broilers for poultry inspection sites was suggested: bilirubin is detectable from pericardial fluid and the carcass is in a state of yellow color change.
authors.affiliation: Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Mizobe
authors.forename: F
authors.initials: F
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Kumamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kanda
authors.forename: H
authors.initials: H
authors.lastname: Seguchi
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000032:analysis*; D001664:Biliverdine / Q000032:analysis*; D002645:Chickens; D002779:Cholestasis / Q000097:blood / Q000453:epidemiology / Q000662:veterinary; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004196:Disease Outbreaks / Q000662:veterinary*; D006520:Hepatitis, Animal / Q000175:diagnosis / Q000453:epidemiology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007565:Jaundice / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D008099:Liver / Q000473:pathology*; D010496:Pericardium; D011201:Poultry Diseases
chemical_list: D001664:Biliverdine; D001663:Bilirubin