Seroepidemiological studies of cutaneous leishmaniasis in the Campeche state of México.
Seroepidemiological studies of cutaneous leishmaniasis were carried out in 169 individuals in a rural area of the Campeche state of México. Fifty showed cutaneous lesions suggestive of leishmaniasis, 70% were parasite positive and 96% skin test positive. An overall 40% positivity to skin test with Montenegro's antigen was found. Most of the affected individuals were males from 11 to 30 years-old. Antibodies were determined by immunofluorescent antibody test (IFA) and by Western blot. Two antigen preparations were used, one from a Leishmania mexicana strain which produced localized cutaneous leishmaniasis (LCL) and the other from a diffuse cutaneous leishmaniasis (DCL). In the general population from the area of study 19% gave positive IFA tests with DCL antigen and 20% with LCL antigen while for the patients 67% gave positive IFA tests with DCL and 71% with LCL. By Western blot analysis most of the patients recognized more antigens in the DCL than in the LCL strain. In the DCL strain 78% of patients recognized a 105 kDa, 34% a 139 kDa, 28% a 117 kDa and 26% a 205 kDa MW antigen. In the LCL strain 40% of patients recognized a 205 kDa and 22% a 175 kDa antigens.
Monroy-Ostria
A
A
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas del IPN, México city, México.
Sosa-Cabrera
T
T
Rivas-Sanchez
B
B
Ruiz-Tuyu
R
R
Mendoza-Gonzalez
A R
AR
Favila-Castillo
L
L
D000328:Adult; D000368:Aged; D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007892:Leishmania braziliensis / Q000276:immunology; D007894:Leishmania mexicana / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000453:epidemiology*; D008297:Male; D008800:Mexico / Q000453:epidemiology; D016036:Seroepidemiologic Studies
D000953:Antigens, Protozoan