Evidence for a direct interaction of superoxide anion radical with carnosine.
Using photochemiluminescence, the interaction between carnosine and superoxide anion was measured directly. Carnosine at physiological concentrations decreased the amplitude of luminol chemiluminescence like superoxide dismutase (SOD) did, and prolonged the lag-period of the chemiluminescence similar to the effect of ascorbic acid. From the interaction of nitro blue tetrazolium with superoxide anion generated by the xanthine oxidase system, the constant for interaction of carnosine with 02-. was calculated to be 10(5) M-1.sec-1. The possible biological significance of the quenching of superoxide anion by carnosine is discussed.
Klebanov
G I
GI
Russian State Medical University, Moscow, Russia.
Teselkin YuO
Babenkova
I V
IV
Popov
I N
IN
Levin
G
G
Tyulina
O V
OV
Boldyrev
A A
AA
Vladimirov YuA
D002336:Carnosine / Q000378:metabolism* / Q000494:pharmacology; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D013481:Superoxides; D009580:Nitroblue Tetrazolium; D008165:Luminol; D002336:Carnosine; D014969:Xanthine Oxidase