The role of U5 snRNP in pre-mRNA splicing.
The current model for the function of the U5 small nuclear ribonucleoprotein particle (snRNP) in the spliceosome proposes that U5 carries binding sites for the 5' and 3' exons, allowing the spliceosome to 'tether' the 5' exon intermediate produced by the first catalytic step and align it with the 3' exon for the second step. Functional analysis of U5 snRNA in cis-spliceosomes has provided support for this model, and data from nematode and trypanosome splicing systems suggest that U5 or a U5-like snRNA performs a similar role in trans-splicing.
Newman
A J
AJ
MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, UK. newman@mrc-lmb.com.ac.uk
D000818:Animals; D002384:Catalysis; D005091:Exons; D006801:Humans; D007438:Introns; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D017415:Ribonucleoprotein, U5 Small Nuclear / Q000502:physiology*; D017381:Spliceosomes / Q000502:physiology
D012322:RNA Precursors; D017415:Ribonucleoprotein, U5 Small Nuclear