Reduction of subacute lethal radiotoxicity of polonium-210 in rats by chelating agents.
The protective effect of N,N'-di(2-hydroxyethyl)ethylene-diamine-N,N'-biscarbodithioate (HOEtTTC) against the subacute lethal radiotoxicity of polonium-210 was investigated in a survival study and by histopathological and haematological examinations of some organs and tissues in Sprague-Dawley rats. This effect was compared with that of N,N'-diethylamine-N-carbodithioate (diethy dithiocarbamate, DDTC). In the survival study, rats injected in intravenously solely with a lethal amount of 210Po (1.45 MBq kg-1 body mass) died within 14-44 days while 90% of rats treated with HOEtTTC survived for 5 months until sacrificed. When treated with DDTC all rats died within 36-93 days. In the histopathological examination, relevant changes resulting from incorporation of 210Po were found in lymph nodes, thymus and humeral bone marrow. After the treatment with HOEtTTC no pathological changes were observed. In the haematological examination, severe reduction in blood and femoral bone marrow (BM) cell counts was revealed in rats injected with 210Po. This reduction was reversed by treatment with HOEtTTC. Treatment with DDTC led only to partial recovery of blood and BM cell count. In conclusion, under the conditions of the experiment only HOEtTTC was fully effective in reducing subacute lethal radiotoxicity of 210Po.
Rencová
J
J
National Institute of Public Health, Centre of Industrial Hygiene and Occupational Diseases, Praha, Czech Republic.
Svoboda
V
V
Holusa
R
R
Volf
V
V
Jones
M M
MM
Singh
P K
PK
D000818:Animals; D001769:Blood / Q000528:radiation effects; D001853:Bone Marrow / Q000528:radiation effects; D002614:Chelating Agents / Q000627:therapeutic use*; D004050:Ditiocarb / Q000627:therapeutic use; D004347:Drug Interactions; D005260:Female; D011059:Polonium / Q000633:toxicity*; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002614:Chelating Agents; D011837:Radiation-Protective Agents; D004050:Ditiocarb; D011059:Polonium