Salivary gland transplantation: a canine model.
Impaired salivary function with resultant severe dryness of the mouth, or xerostomia, may occur in association with a variety of systemic disorders or therapies. No adequate treatment exists for this debilitating condition, which impedes normal oral function, in particular alimentation and phonation. This study explores the feasibility of salivary gland autotransplantation, using a canine model. A salivary gland with its duct and surrounding blood vessels still attached was excised and reimplanted in the dog's thigh by anastomosing the graft's blood vessels to the femoral artery and vein. The duct was sutured to an artificial orifice cut in the thigh's skin, from which the saliva was collected. Salivary secretion was induced by a single intravenous bolus of pilocarpine (5 mg). Preoperative (normal) salivation was measured by collecting saliva from the gland in situ. Periodic functional studies showed normal saliva production during the first month after grafting, after which the salivary flow was reduced by 35% over the next 2 months. This reduction was interpreted as a sign of disuse atrophy resulting from the lack of autonomic innervation. To overcome this impediment, oral pilocarpine (5 mg/day) was administered to the recipient dog, after which normal levels of saliva were excreted through the graft during the 3-month follow-up period. The quality of the graft saliva was assessed by its protein and electrolyte levels, which showed close to normal values.
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Department of Surgery, The Hebrew University-Hadassah Faculty of Medicine, Jerusalem, Israel.
Nitzan
D W
DW
Shiloni
E
E
Neuman
A
A
Marmary
Y
Y
D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D008297:Male; D008954:Models, Biological; D011188:Potassium / Q000032:analysis; D012469:Salivary Glands / Q000737:chemistry / Q000637:transplantation*; D012472:Salivation / Q000502:physiology; D012964:Sodium / Q000032:analysis; D014182:Transplantation, Autologous / Q000379:methods; D014987:Xerostomia / Q000601:surgery
D012964:Sodium; D011188:Potassium