Stable radiobiological features in a genetically unstable glioblastoma cell line.
In a human glioblastoma (grade IV) cell line, A7, the change in chromosome number, growth characteristics, radiosensitivity and DNA-repair capacity were determined during continuous culture over approximately 1300 generations. The median chromosome number fell from 101 to 85 while a remarkable variability was retained. The net population doubling time shortened from 21 to 16 h but no alterations were detected either in radiosensitivity or DNA-repair capacity, as measured by colony and plasmid-reconstitution assays respectively. Reviewing radiosensitivity data from the literature, it is considered that the relative radioresistance of glioblastomas might be inherited from the normal tissue from which they are derived.
Bartkowiak
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Universitätsklinik Ulm, Germany.
Röttinger
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EM
D002455:Cell Division / Q000502:physiology / Q000528:radiation effects; D002877:Chromosomes, Human; D004260:DNA Repair; D018450:Disease Progression; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000528:radiation effects*