title: Antileukoprotease: an endogenous protein in the innate mucosal defense against fungi.
abstract: Previous studies have suggested that endogenous protease inhibitors may participate in the mucosal host defense. Antileukoprotease (ALP) is an important protease inhibitor found on various mucosal surfaces, including those of the respiratory and genital tracts. This study reports on the antimicrobial activity of recombinant (r) ALP toward the human fungal pathogens Aspergillus fumigatus and Candida albicans. rALP expressed pronounced fungicidal activity toward metabolically active A. fumigatus conidia and C. albicans yeast cells; however, metabolically quiescent A. fumigatus conidia were totally resistant. In contrast with the protease inhibitory activity of rALP, the fungicidal activity was localized primarily in the NH2-terminal domain. On a molar base, the fungicidal activity of rALP was comparable with that of human defensins and lysozyme. In addition, rALP caused inhibition of C. albicans yeast cell growth. By exhibiting antifungal activity, ALP may play an important role in the innate mucosal defense against human pathogenic fungi.
authors.affiliation: Department of Allergology, Groningen University Hospital, Netherlands.
authors.forename: J F
authors.initials: JF
authors.lastname: Tomee
authors.forename: P S
authors.initials: PS
authors.lastname: Hiemstra
authors.forename: R
authors.initials: R
authors.lastname: Heinzel-Wieland
authors.forename: H F
authors.initials: HF
authors.lastname: Kauffman
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D006801:Humans; D018928:Immunity, Mucosal; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000276:immunology / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D011993:Recombinant Fusion Proteins; D015842:Serine Proteinase Inhibitors