title: Idiorrhythmic dose-rate variability in dietary zinc intake generates a different response pattern of zinc metabolism than conventional dose-response feeding.
abstract: We compared the effects of idiorrhythmic dose-rate feeding and conventional dose-response on the induction of intestinal metallothionein (iMT), expression of aortal heat-shock protein mRNA (HSP70mRNA) induced by restraint stress, and accumulation of Zn in the femur and incisor of young growing male rats. An idiorrhythmic approach requires that the average dietary Zn concentration (modulo, M) over the whole experiment (epoch, E) is kept constant across different groups. This is done by adjusting the Zn concentration of the supplemented diet supplied to compensate for the reduction in the number of days on which Zn-supplemented diet is fed, the latter being spread evenly over the experiment. Idiorrhythms involve offering the diet with n times the overall Zn concentration (M) only every nth day with Zn-deficient diet offered on other days. Idiorrythmic Zn dose-rate feeding changed Zn accumulation in the femur and incisor in a complex bi-modal fashion, indicating that metabolic efficiency of dietary Zn is not constant but depends on Zn dose-rate. In contrast to feeding Zn in the conventional dose-response scheme, iMT and HSP70mRNA were not affected by idiorrhythmic dose-rate feeding. Idiorrhythmic cycling in dietary Zn load posed no risk of a biochemical overload nor caused the animals to be stressed. Idiorrhythmic dose-rate feeding brings the dimension of time to the conventional dose-response model.
authors.affiliation: Institute for Medical Research and Occupational Health, Zagreb, Croatia.
authors.forename: B
authors.initials: B
authors.lastname: Momcilović
authors.forename: P G
authors.initials: PG
authors.lastname: Reeves
authors.forename: M J
authors.initials: MJ
authors.lastname: Blake
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D015152:Blotting, Northern; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005269:Femur / Q000378:metabolism; D006128:Growth / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007180:Incisor / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological; D015032:Zinc / Q000008:administration & dosage* / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D008668:Metallothionein; D015032:Zinc