Induction of tissue-specific autoimmune prostatitis with prostatic acid phosphatase immunization: implications for immunotherapy of prostate cancer.
Prostatic acid phosphatase (PAP) is uniquely expressed in prostatic tissue and prostate cancer. In this study, the immunogenicity of PAP was investigated in a male rat model. We show that immunization with recombinant rat or human PAP in CFA leads to a significant Ab response, but does not generate CTL or result in autoimmune prostatitis. In contrast, immunization with recombinant vaccinia expressing human PAP, but not rat PAP, generates a CTL response and tissue-specific prostatitis in the absence of detectable PAP-specific Abs. These findings suggest that a cellular immune response to PAP, rather than Abs, mediates destructive autoimmune prostatitis. Thus, xenogeneic forms of PAP are a new tool for the induction of prostate-specific immunity and may prove useful for the immunotherapy of prostate cancer.
Fong
L
L
Stanford University School of Medicine, CA 94305, USA.
Ruegg
C L
CL
Brockstedt
D
D
Engleman
E G
EG
Laus
R
R
D000135:Acid Phosphatase / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000818:Animals; D001327:Autoimmune Diseases / Q000201:enzymology / Q000209:etiology* / Q000628:therapy*; D006801:Humans; D016233:Immunotherapy, Active; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D008297:Male; D009928:Organ Specificity / Q000276:immunology; D011467:Prostate / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000276:immunology* / Q000628:therapy*; D011472:Prostatitis / Q000201:enzymology / Q000209:etiology* / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology
D014614:Vaccines, Synthetic; D000135:Acid Phosphatase