PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes.
A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were determined, and in 144 of 149 cases (97%) the results were congruous. Strains of types that were not included in the panel of capture probes were emm genotyped by sequencing.
Saunders
N A
NA
HRL, London, United Kingdom. nsaunder@phls.co.uk
Hallas
G
G
Gaworzewska
E T
ET
Metherell
L
L
Efstratiou
A
A
Hookey
J V
JV
George
R C
RC
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D018070:Biological Specimen Banks; D002352:Carrier Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012015:Reference Standards; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013297:Streptococcus pyogenes / Q000145:classification*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C014689:streptococcal M protein