[Effects of PDGF AB on extracellular matrix synthesis by human mammary fibroblasts].
PDGF AB effect on extracellular matrix protein synthesis and release by a primary culture of non transformed human mammary fibroblasts was analyzed. Primarily PDGF AB induced a modified protein distribution, with a global enhancement of secreted proteins as compared to intracytoplasmatic proteins. Furthermore, an increase in synthesis and release of the extracellular matrix proteins such as fibronectin and type I and IV collagens upon PDGF AB treatment with specific immunoprecipitation was demonstrated.
Palumbo
C
C
Dipartimento di Medicina Sperimentale e Patologia, Università degli Studi La Sapienza, Roma.
Vasaturo
F
F
Zelli
G
G
Cardelli
P
P
Rubinacci
G
G
Modesti
M
M
Scarpa
S
S
D000368:Aged; D001345:Autoradiography; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000187:drug effects; D005260:Female; D005319:Fetal Hemoglobin / Q000378:metabolism*; D018226:Fibroadenoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011233:Precipitin Tests
D016326:Extracellular Matrix Proteins; D010982:Platelet-Derived Growth Factor; D005319:Fetal Hemoglobin