title: Determination of diazolidinyl urea in a topical cream by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic method for the determination of diazolidinyl urea (DU) in a cream formulation is described. The aqueous phase of the emulsion was separated by centrifugation, removed, filtered, diluted and applied onto the HPLC system. DU was detected by ultraviolet absorption at a wavelength of 214 nm. The calibration curve was linear over the range of 79-553 micrograms/ml, and identical when determined on consecutive days. The relative standard deviation for repeat determinations was less than 0.5%. Recoveries were 97.74-101.72%. This analytical method is useful for quantitation of DU in cream formulations.
authors.affiliation: Pharmaceutics Division, College of Pharmacy, University of Texas, Austin 78712-1074, USA.
authors.forename: R O
authors.initials: RO
authors.lastname: Williams
authors.forename: V
authors.initials: V
authors.lastname: Mahaguna
authors.forename: M
authors.initials: M
authors.lastname: Sriwongjanya
mesh_terms: D000287:Administration, Topical; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014508:Urea / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000890:Anti-Infective Agents; D014508:Urea; C044982:diazolidinylurea