title: Intracellular acidification induces Cl/HCO3 exchange activity in the basolateral membrane of beta-intercalated cells of the rabbit cortical collecting duct.
abstract: High speed video imaging microscopy and the pH-sensitive fluorophore2',7',-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) were used to examine acid-base functions of beta-intercalated cells of the rabbit cortical collecting duct. The presence of intercalated cells was established and the properties of apical and basolateral acid-base transporters assessed by monitoring cell pH during acid loading and luminal and basolateral ion substitutions. We showed that treatment of beta-intercalated cells with ammonium chloride (20 mm) induced a profound decrease of their intracellular pH from 6.98 +/- 5.93 +/- 0.08. pH recovery occurred after different lag periods ranging between 2 to 15 min (0.22 +/- 0. 04 dpH/dt). We demonstrated that this pH recovery mechanism was independent of basolateral Na+ and apical HCO-3 and K+. It was also not affected by apical and basolateral addition of NEM, by basolateral DIDS and by apical application of the H-KATPase inhibitor SCH28080. The process of pH recovery was however, critically dependent on basolateral HCO-3. These results are best explained by acid-induced insertion and/or activation of chloride-bicarbonate exchangers that are functional properties with their apical analogues.
authors.affiliation: Department de Biologie Cellulaire et Moleculaire, C.E.N. Saclay, Department de Biologie, 91191 Gif sur Yvette Cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Merot
authors.forename: G
authors.initials: G
authors.lastname: Giebisch
authors.forename: J
authors.initials: J
authors.lastname: Geibel
mesh_terms: D000818:Animals; D017920:Antiporters / Q000378:metabolism*; D001485:Basement Membrane / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism*; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides / Q000378:metabolism*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007672:Kidney Cortex / Q000166:cytology / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000378:metabolism*; D018715:Microscopy, Video; D011817:Rabbits
chemical_list: D017920:Antiporters; D001639:Bicarbonates; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides; D005452:Fluoresceins; D005456:Fluorescent Dyes; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein