[Development of an experimental rat model of intraabdominal abscess by Escherichia coli alone. I. Materials for abscess formation].
To develop a new animal model of intraabdominal abscess by Escherichia coli alone, we reevaluated anaerobes and other additions which had been believed necessary to produce an intraabdominal abscess. We took the method of bacterial implantation by insertion of a double gelatin capsules containing microbes and the additions into the peritoneal cavity of male Wister rats. We examined the requirement of causative bacteria for an abscess including both aerobes and anaerobes, sterilized feces, and barium sulfate. It has been proven that a simple and well reproducible intraabdominal abscess can be developed without fail at the seventh day after inoculation although anaerobic bacteria, sterilized feces, and barium sulfate are not used. However, we have failed to produce an abscess without sterilized gauze fiber which should be a core of an abscess and is used instead of sterilized feces. This animal model will contribute to a major simplification of the original one heretofore in use, and is expected to serve as an aid to elucidate the mechanisms of abscess formation.
Tabata
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Osaka Prefectural Senshu Critical Care Medical Center.
Sugimoto
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D018784:Abdominal Abscess / Q000382:microbiology; D000818:Animals; D001431:Bacteriological Techniques; D001458:Bandages; D001466:Barium Sulfate; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D005780:Gelatin; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D001466:Barium Sulfate; D005780:Gelatin