title: The assimilatory nitrate reductase from the phototrophic bacterium, Rhodobacter capsulatus E1F1, is a flavoprotein.
abstract: The assimilatory nitrate reductase from the phototrophic bacterium Rhodobacter capsulatus has been purified to electrophoretic homogeneity and its molecular and kinetic parameters determined. The native nitrate reductase is a dimer of 144 kDa composed of two subunits of 46 and 95 kDa. The purified enzyme catalyzes the electron transfer from NADH, reduced bromophenol blue or reduced viologens to nitrate. The nitrate reductase contains 1 mol FAD per mole of enzyme and also reduces cytochrome c or dichlorophenol indophenol with NADH as the electron donor. The diaphorase activity is located in the small subunit.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Blasco
authors.forename: F
authors.initials: F
authors.lastname: Castillo
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Luque
mesh_terms: D015086:2,6-Dichloroindophenol / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D008058:Dihydrolipoamide Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D019281:Dimerization; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry; D005420:Flavoproteins / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010084:Oxidation-Reduction; D011487:Protein Conformation; D016354:Rhodobacter capsulatus / Q000201:enzymology*; D013053:Spectrophotometry
chemical_list: D003574:Cytochrome c Group; D005420:Flavoproteins; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D015086:2,6-Dichloroindophenol; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D008058:Dihydrolipoamide Dehydrogenase