Thymidylate synthase is a predictor for response and resistance in hepatic artery infusion chemotherapy.
The value of intratumoral thymidylate synthase (TS) quantitation as a predictive parameter for hepatic artery infusion (HAI) chemotherapy in patients with colorectal liver metastases was investigated. Relative TS mRNA levels were determined in 29 tumor samples using a quantitative RT-PCR amplification method. The median level of expression was 3.0 x 10(-3) (no units) and varied considerably among the tumors over a range of 135-fold. Patients with low TS levels were 4.1-fold more likely to respond (P < 0.03) compared to patients with high TS levels. Our results indicate that TS quantitation is a valuable predictive marker for tumor response to HAI therapy.
Kornmann
M
M
Department of General Surgery, University of Ulm, Germany.
Link
K H
KH
Lenz
H J
HJ
Pillasch
J
J
Metzger
R
R
Butzer
U
U
Leder
G H
GH
Weindel
M
M
Safi
F
F
Danenberg
K D
KD
Beger
H G
HG
Danenberg
P V
PV
D000328:Adult; D000368:Aged; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000627:therapeutic use*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage / Q000627:therapeutic use*; D015415:Biomarkers; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000201:enzymology; D004334:Drug Administration Schedule; D019008:Drug Resistance, Neoplasm; D015251:Epirubicin / Q000008:administration & dosage; D005260:Female; D005467:Floxuridine / Q000008:administration & dosage / Q000627:therapeutic use*; D005472:Fluorouracil / Q000008:administration & dosage / Q000627:therapeutic use*; D006499:Hepatic Artery; D006801:Humans; D007261:Infusions, Intra-Arterial; D002955:Leucovorin / Q000008:administration & dosage / Q000627:therapeutic use; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D008942:Mitoxantrone / Q000008:administration & dosage; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis; D015996:Survival Rate; D013940:Thymidylate Synthase / Q000032:analysis / Q000096:biosynthesis*; D014158:Transcription, Genetic
D000964:Antimetabolites, Antineoplastic; D015415:Biomarkers; D012333:RNA, Messenger; D005467:Floxuridine; D015251:Epirubicin; D008942:Mitoxantrone; D013940:Thymidylate Synthase; D002955:Leucovorin; D005472:Fluorouracil