Sequence of the Bacillus subtilis genome region in the vicinity of the lev operon reveals two new extracytoplasmic function RNA polymerase sigma factors SigV and SigZ.
Two regions with sizes 18,900 and 25,400 bp, which join previously known contigs containing levRDEFG, aadK and blt genes near 235 degrees of the Bacillus subtilis chromosome, were sequenced. Among others, two genes, which encode proteins homologous to RNA polymerase sigma-factors, were identified within this region. The gene products designated SigV and SigZ, show the highest homology with sigma-factors encoded by the gene carQ of Myxococcus xanthus and sigX (formerly orfX20) of B. subtilis, correspondingly. All sigma-factors which show statistically significant homology to SigV and SigZ, belong to the ECF (extracytoplasmic functions) subfamily. SigV and SigZ do not have N-terminal sequence which prevents such proteins from binding to DNA without RNA polymerase core enzyme.
Sorokin
Alexei
A
QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
Bolotin
Alexandre
A
QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
Purnelle
Helmut
H
QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
Hilbert
Helmut
H
QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
Lauber
Juergen
J
QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
Düsterhöft
Andreas
A
QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
Ehrlich
S Dusko
SD
Laboratoire de GéAnéAtique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France.
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D018387:Codon, Initiator / Q000235:genetics; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000235:genetics*
D001426:Bacterial Proteins; D018387:Codon, Initiator; D004269:DNA, Bacterial; C109130:SigV protein, Bacillus subtilis; C109131:SigZ protein, Bacillus subtilis; D012808:Sigma Factor