[Chemotactic activity of human peripheral eosinophils toward leukotriene E4].
We studied the chemotactic activity of isolated human peripheral eosinophils toward leukotriene (LT) E4. We obtained eosinophil suspensions by the method of CD16 negative selection. Eosinophil chemotactic activities toward platelet activating factor (PAF, 10(-6) M) and LTE4 (10(-7) M, 10(-6) M, 10(-5) M) were studied using a modified Boyden Chamber method. Eosinophils migrated significantly toward PAF and LTE4 (10(-7) M, 10(-6) M). The chemotactic activity toward LTE, was most potential at 10(-6) M. These results suggest that LTE4 is an eosinophil chemoattractant.
Imai
T
T
First Department of Internal Medicine, School of Medicine, Showa University.
Okamoto
M
M
Horikoshi
S
S
Sugeta
A
A
Idaira
K
K
Kokubu
F
F
Mita
S
S
Adachi
M
M
D002634:Chemotaxis, Leukocyte; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D017999:Leukotriene E4; D008297:Male; D010972:Platelet Activating Factor
D010972:Platelet Activating Factor; D017999:Leukotriene E4