title: The effect of ashing on cells: spectromicroscopy of physiological elements.
abstract: We analyzed the effects of cold oxygen plasma ashing of neurobiological specimens on different elements with synchrotron spectromicroscopy. Our results demonstrate that while carbon is almost completely removed, phosphorus, calcium, potassium, sulfur, and, to some extent, nitrogen are retained and their relative concentration is enhanced.
authors.affiliation: Institut de Physique Appliquée, Ecole Polytechnique Fédérale, Lausanne, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: De Stasio
authors.forename: M
authors.initials: M
authors.lastname: Capozi
authors.forename: T C
authors.initials: TC
authors.lastname: Droubay
authors.forename: D
authors.initials: D
authors.lastname: Mercanti
authors.forename: M T
authors.initials: MT
authors.lastname: Ciotti
authors.forename: G F
authors.initials: GF
authors.lastname: Lorusso
authors.forename: R
authors.initials: R
authors.lastname: Andres
authors.forename: T
authors.initials: T
authors.lastname: Suda
authors.forename: P
authors.initials: P
authors.lastname: Perfetti
authors.forename: B P
authors.initials: BP
authors.lastname: Tonner
authors.forename: G
authors.initials: G
authors.lastname: Margaritondo
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002531:Cerebellum / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D011188:Potassium / Q000737:chemistry; D051381:Rats; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D013455:Sulfur / Q000737:chemistry; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D010758:Phosphorus; D013455:Sulfur; D002244:Carbon; D009584:Nitrogen; D011188:Potassium; D010100:Oxygen; D002118:Calcium