The matrix metalloproteinase-14 (MMP-14) gene is structurally distinct from other MMP genes and is co-expressed with the TIMP-2 gene during mouse embryogenesis.
The matrix metalloproteinases (MMPs) are a family of zinc-containing matrix degrading endopeptidases. A subfamily of membrane type (MT) -MMPs has been described recently. We have determined the structure of the gene (Mmp14) encoding the first MT-MMP to be described, MT1-MMP (MMP-14), and mapped it to mouse chromosome 14. The mouse MMP-14 protein is encoded by ten exons. The novel C-terminal peptide domains of MMP-14 are encoded by a single large exon that also encodes the 3'-untranslated region. The structure of the exons encoding the catalytic domain and pro-domain of MMP-14 is distinct from previously described MMP genes, whereas the exons encoding the hemopexin-like domains are similar to those of most other MMP genes. Mmp14 and the gene for tissue inhibitor of metalloproteinases-2 (Timp2) show a temporally and spatially co-regulated expression during mouse development. They are co-expressed during vascular and urogenital development and during the development of osteocartilaginous and musculotendinous structures. The stringent co-expression of these two genes suggests common regulatory pathways that may have important functional implications for the activation of pro-gelatinase A in health and disease.
Apte
S S
SS
Department of Biomedical Engineering, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA. aptes@bme.ri.ccf.org
Fukai
N
N
Beier
D R
DR
Olsen
B R
BR
D000818:Animals; D001665:Binding Sites; D015152:Blotting, Northern; D001842:Bone and Bones / Q000196:embryology / Q000201:enzymology; D002874:Chromosome Mapping; D002875:Chromosomes; D004622:Embryo, Mammalian / Q000201:enzymology*; D005314:Embryonic and Fetal Development; D005091:Exons; D015870:Gene Expression; D007438:Introns; D053511:Matrix Metalloproteinase 14; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000196:embryology / Q000201:enzymology; D011480:Protease Inhibitors / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics*
C507096:Mmp14 protein, mouse; D011480:Protease Inhibitors; D019716:Tissue Inhibitor of Metalloproteinase-2; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D053511:Matrix Metalloproteinase 14