Molecular cloning of rat p38 mitogen-activated protein kinase and it's osmotic regulation in rat kidney.
Rat p38 mitogen-activated protein (MAP) kinase cDNA was isolated from rat kidney cDNA library using a PCR cloning strategy. The deduced amino acid sequence consists of 360 amino acids and shares 95.3% similarity with human p38 MAP kinase. The message for rat p38 MAP kinase was about 3.4 kilobases and was highly expressed in the kidney. In water-deprived rat kidneys, the steady-state levels of p38 MAP kinase mRNA increased about 2.7-fold as compared with those of control rats. This result suggests that p38 MAP kinase may play an important role in the osmoregulation in the kidney.
Yoshida
T
T
Fourth Department of Internal Medicine, Tokyo Women's Medical College, Japan.
Sone
M
M
Ogawa
T
T
Nihei
H
H
Ozasa
H
H
Tsukada
K
K
Horikawa
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D008297:Male; D020928:Mitogen-Activated Protein Kinases; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016415:Sequence Alignment; D014882:Water-Electrolyte Balance; D048051:p38 Mitogen-Activated Protein Kinases
D018076:DNA, Complementary; D012333:RNA, Messenger; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases