title: Interaction of bromocresol green with different serum albumins studied by fluorescence quenching.
abstract: The binding of bromocresol green to bovine serum albumin at micromolar concentrations leads to quenching of protein fluorescence. This property has been used here to study interaction of bromocresol green with bovine serum albumin as a function of pH and ionic strength. The transformation of fluorescence quench data obtained with bromocresol green into Scatchard plots yielded an association constant of 3.06 x 10(7) 1 M-1 and a binding capacity of about 1.0. The affinity of bromocresol green for bovine serum albumin remains virtually unchanged between pH 4.0 and 8.0 but decreases by about 7 fold with increase in ionic strength from 0.01 to 1.0. Six other serum albumins obtained from cat, dog, human, pig and sheep have also been studied for bromocresol green binding. Although all the albumins studied bind bromocresol green, they show considerable differences in their affinities towards the dye. It appears that despite a great degree of overall similarity in their structure and conformation, serum albumins from different species differ in their ligand binding properties.
authors.affiliation: Department of Biochemistry, J.N. Medical College, A.M.U., Aligarh, India.
authors.forename: V D
authors.initials: VD
authors.lastname: Trivedi
authors.forename: I
authors.initials: I
authors.lastname: Saxena
authors.forename: M U
authors.initials: MU
authors.lastname: Siddiqui
authors.forename: M A
authors.initials: MA
authors.lastname: Qasim
mesh_terms: D000818:Animals; D001665:Binding Sites; D001961:Bromcresol Green / Q000378:metabolism*; D002415:Cats; D004285:Dogs; D006041:Goats; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D012709:Serum Albumin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012756:Sheep; D013050:Spectrometry, Fluorescence; D013552:Swine
chemical_list: D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D001961:Bromcresol Green