Mapping of an allergenically important determinant of grass group I allergens.
This report documents the mapping of the second major epitope, previously described as site D, of grass group I allergens to residues 23-35 of meadow fescue group I (STFYGKPTGAGPK). Mapping was accomplished by screening fractions from a meadow fescue group I tryptic digest for peptide(s) that inhibit the ability of monoclonal antibody 24.64 (specific for site D) to bind to immobilized group I allergen. One such peptide, representing residues 23-35 of meadow fescue group I, was identified. Additional studies with the use of synthetic analogs of this peptide demonstrate that it binds mAb 24.64 directly. Examination of extracts containing group I glycoproteins from seven other species of grass confirms antigenic cross-reactivity due to this peptide. We also report based on protein sequence analysis that the amino terminal segment (which includes the site D epitope) of GpI allergens from seven different grass species is highly conserved and contains two hydroxyproline residues and an N-linked carbohydrate moiety.
Hiller
K M
KM
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599-7290, USA.
Esch
R E
RE
Klapper
D G
DG
D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D006909:Hydroxyproline / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D006109:Poaceae / Q000276:immunology*; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D000911:Antibodies, Monoclonal; D010455:Peptides; D006909:Hydroxyproline