title: Cloning of the phosphonoacetate hydrolase gene from Pseudomonas fluorescens 23F encoding a new type of carbon-phosphorus bond cleaving enzyme and its expression in Escherichia coli and Pseudomonas putida.
abstract: The phnA gene encoding a novel carbon-phosphorus bond cleavage enzyme, phosphonoacetate hydrolase, from Pseudomonas fluorescens 23F was cloned and expressed in Escherichia coli and Pseudomonas putida. It conferred on the latter host the ability to mineralize phosphonoacetate but on the former the ability to utilize it as sole phosphorus source only. The nucleotide and deduced amino acid sequences of the phnA gene showed no significant homology with any data bank accessions.
authors.affiliation: The Questor Centre and School of Biology and Biochemistry, The Queen's University of Belfast, UK.
authors.forename: A N
authors.initials: AN
authors.lastname: Kulakova
authors.forename: L A
authors.initials: LA
authors.lastname: Kulakov
authors.forename: J P
authors.initials: JP
authors.lastname: Quinn
mesh_terms: D000469:Alkaline Phosphatase; D000595:Amino Acid Sequence; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000235:genetics; D016958:Pseudomonas putida / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017421:Sequence Analysis
chemical_list: D011994:Recombinant Proteins; D010758:Phosphorus; D002244:Carbon; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; C097254:phosphonoacetate hydrolase