B cell subpopulations separated by CD27 and crucial collaboration of CD27+ B cells and helper T cells in immunoglobulin production.
B cell immunoglobulin production is regulated by helper T cells through direct interaction and secreted cytokines. In the present study, we functionally analyzed CD27 in cord and peripheral blood B cells. Adult peripheral blood B cells were separated into CD27+ and CD27- cells, which differed in their morphology. Cord blood B cells did not express CD27, and CD27 expression on peripheral blood B cells increased with age. Only CD27+ B cells had the ability to produce immunoglobulin, which was increased by contact with a tumor necrosis factor-related transmembrane ligand, CD70. Adult peripheral blood CD27+ B cells can be further subdivided into two discrete subtypes: IgD- CD27+ and IgD+ CD27+ B cells. IgD- CD27+ B cells produce IgG, IgM and IgA, whereas IgD+ CD27+ B cells predominantly produce IgM. The addition of activated CD4+ CD45RO T cells expressing CD70 caused down-regulation of CD27 expression on activated B cells, and this down-modulation was completely blocked by anti-CD70 monoclonal antibody, indicating direct T-B cell contact via CD27/CD70. The triggering via CD27 and CD40 additively increased the immunoglobulin production under Staphylococcus aureus Cowan strain plus interleukin-2 stimulation. Taken together, our findings demonstrate that peripheral blood B cells are separated into subpopulations by CD27 and IgD expression and that CD27+ B cells produce large amounts of immunoglobulin by interaction with the CD70 molecule.
Agematsu
K
K
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
Nagumo
H
H
Yang
F C
FC
Nakazawa
T
T
Fukushima
K
K
Ito
S
S
Sugita
K
K
Mori
T
T
Kobata
T
T
Morimoto
C
C
Komiyama
A
A
D000328:Adult; D015703:Antigens, CD; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D001483:Base Sequence; D053282:CD27 Ligand; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D019013:CD40 Antigens / Q000378:metabolism; D002469:Cell Separation; D002648:Child; D002675:Child, Preschool; D002999:Clone Cells; D017931:DNA Primers / Q000235:genetics; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D006801:Humans; D007072:Immunoglobulin D / Q000378:metabolism; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007223:Infant; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008211:Lymphocyte Cooperation; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism*
D015703:Antigens, CD; D053282:CD27 Ligand; D019013:CD40 Antigens; C505771:CD70 protein, human; D017931:DNA Primers; D007072:Immunoglobulin D; D007136:Immunoglobulins; D008565:Membrane Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D017493:Leukocyte Common Antigens