title: In vivo and in vitro hepatic 31P magnetic resonance spectroscopy and electron microscopy of the cirrhotic liver.
abstract: In vivo 31P magnetic resonance spectroscopy (MRS) provides direct biochemical information on hepatic metabolic processes. To assess in vivo changes in hepatic 31P MRS in liver transplant candidates, we studied 31 patients with cirrhosis of varying aetiology; 14 with compensated cirrhosis (Pugh's score < or = 7) and 17 with decompensated cirrhosis (Pugh's score > or = 8). Underlying cellular abnormalities were characterised using in vitro 31P MRS and electron microscopy. In vitro spectra were obtained from liver extracts, freeze-clamped at recipient hepatectomy, from all subjects. Electron microscopy of liver tissue was also performed in 17 cases. Relative to nucleotide triphosphates, elevations in phosphomonoesters and reductions in phosphodiesters were observed in vivo with worsening liver function. In vitro spectra showed elevated phosphoethanolamine and phosphocholine, and reduced glycerophosphorylethanolamine and glycerophosphorylcholine, mirroring the in vivo changes, but no distinction was noted between compensated and decompensated cirrhosis. With electron microscopy, functional decompensation was associated with reduced endoplasmic reticulum in parenchymal liver disease, but elevated levels in biliary cirrhosis. We conclude that in vivo spectral abnormalities in cirrhosis are consistent with alterations in phospholipid metabolism and quantity of endoplasmic reticulum. However, in individual patients the biopsy results do not always mirror in vivo findings.
authors.affiliation: Robert Steiner NMR Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
authors.forename: S D
authors.initials: SD
authors.lastname: Taylor-Robinson
authors.forename: J
authors.initials: J
authors.lastname: Sargentoni
authors.forename: J D
authors.initials: JD
authors.lastname: Bell
authors.forename: N
authors.initials: N
authors.lastname: Saeed
authors.forename: K K
authors.initials: KK
authors.lastname: Changani
authors.forename: B R
authors.initials: BR
authors.lastname: Davidson
authors.forename: K
authors.initials: K
authors.lastname: Rolles
authors.forename: A K
authors.initials: AK
authors.lastname: Burroughs
authors.forename: H J
authors.initials: HJ
authors.lastname: Hodgson
authors.forename: C S
authors.initials: CS
authors.lastname: Foster
authors.forename: I J
authors.initials: IJ
authors.lastname: Cox
mesh_terms: D000328:Adult; D000368:Aged; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000648:ultrastructure*; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology*; D008111:Liver Function Tests; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009711:Nucleotides / Q000032:analysis; D010755:Organophosphates / Q000032:analysis; D010759:Phosphorus Isotopes; D011517:Prothrombin Time
chemical_list: D009711:Nucleotides; D010755:Organophosphates; D010759:Phosphorus Isotopes