Development of a sensitive method for quantitation of ABT-089 in plasma using fluorescence labeling with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole.
A high-performance liquid chromatography method for the quantitation of ABT-089 [2-methyl-3-(2-(S)-pyrrolidinylmethoxy)pyridine] (I), a new structural type of cholinergic channel modulators (ChCM), is described in this paper using 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) as a fluorescent-labeling reagent. The method combined an optimized liquid-liquid extraction from plasma followed by pre-column derivatization to yield a fluorescence product. The selectivity, sensitivity, and reproducibility of this method were found to be excellent. This method was applied to the determination of ng/ml plasma and tissue levels of ABT-089 and similar compounds in biological samples.
Hui
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YH
Drug Analysis, Abbott Laboratories, Abbott Park, IL 60064, USA.
Carroll
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Marsh
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D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives*; D000818:Animals; D001923:Brain Chemistry; D018678:Cholinergic Agents / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005456:Fluorescent Dyes; D008252:Macaca fascicularis; D018697:Nootropic Agents / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D011725:Pyridines / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D011759:Pyrrolidines / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
D018678:Cholinergic Agents; D005456:Fluorescent Dyes; D018697:Nootropic Agents; D011725:Pyridines; D011759:Pyrrolidines; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; C108326:pozanicline; D009327:4-Chloro-7-nitrobenzofurazan