Transposon tagging in rice.
To develop an efficient gene isolation method for rice we introduced the maize Ac/Ds system into rice. Extensive analysis of their behavior in rice for several generations indicated that Ac and Ds in the presence of Ac transposase gene actively transpose in rice. A wide spectrum of mutations affecting growth, morphogenesis, flowering time and disease resistance have been obtained in the population carrying Ac/Ds and some of them were genetically analyzed. Main efforts are currently being made to isolate genes responsible these mutations. In addition, a number of Ac/Ds were mapped on chromosomes and mapped elements will be used in the future for directed tagging of genes with known chromosomal positions.
Izawa
T
T
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, Ikoma, Japan.
Ohnishi
T
T
Nakano
T
T
Ishida
N
N
Enoki
H
H
Hashimoto
H
H
Itoh
K
K
Terada
R
R
Wu
C
C
Miyazaki
C
C
Endo
T
T
Iida
S
S
Shimamoto
K
K
D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D017343:Genes, Plant; D005819:Genetic Markers; D012275:Oryza / Q000235:genetics*
D004251:DNA Transposable Elements; D005819:Genetic Markers