Improved long-term bone-implant integration. Experiments in transgenic mice overexpressing bovine growth hormone.
Several recent studies have investigated the effects of growth hormone (GH) on the healing of fractures and bone ingrowth, but with conflicting results. The negative results may be due to antibody formation against injected GH or because some experimental models are able to prove only positive GH effects. In this study, we wanted to investigate the effect of GH on implant integration in bone. To avoid potential formation of antibodies against injected GH, we used a model with transgenic mice overexpressing bovine GH (bGH). Titanium implants were inserted in the forehead of the mice. 4 months after insertion, the implants were cut out en bloc with the surrounding bone. The calcified specimens were cut and ground to a thickness of approximately 10 microns. Histomorphometry demonstrated significantly more direct bone-to-metal contact in the transgenic mice than in the nontransgenic littermates. Our findings indicate that systemic administration of GH in humans may improve implant integration in bone.
Morberg
P H
PH
Dept. of Orthopaedics, East Hospital, Inst. for Surgical Sciences, Gothenburg, Sweden.
Isaksson
O G
OG
Johansson
C B
CB
Sandstedt
J
J
Törnell
J
J
D000818:Animals; D018786:Bone Substitutes; D002417:Cattle; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D013006:Growth Hormone / Q000097:blood* / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic / Q000097:blood*; D016348:Osseointegration / Q000187:drug effects*; D013997:Time Factors; D014025:Titanium
D018786:Bone Substitutes; D013006:Growth Hormone; D014025:Titanium