Changes in the V(H) gene repertoire of developing precursor B lymphocytes in mouse bone marrow mediated by the pre-B cell receptor.
The V(H) repertoire on both H chain alleles of normal and lambda5-deficient B lineage cells were analyzed by single-cell PCR. The mu H chains were tested for their capacity to form a pre-B cell receptor. In bone marrow, D-proximal V(H) genes were found preferentially expressed in lambda5-deficient pre-B cells and in a newly identified early c-kit+ cytoplasmic mu H chain+ pre-B cell population of normal mice. Only half of the mu H chains expressed in these cells have the capacity to form a pre-B cell receptor. Representation of the D-proximal V(H) genes was found suppressed on the productive but not on the nonproductive V(H)DJ(H) rearranged alleles of c-kit preB-II cells and splenic lambda5-deficient B cells. More than 95% of the mu H chains expressed in preB-II cells can form a pre-B cell receptor. These results demonstrate that the pre-B cell receptor in normal mice and the B cell receptor in lambda5-deficient mice mediate a shift in the V(H) repertoire.
ten Boekel
E
E
Basel Institute for Immunology, Switzerland.
Melchers
F
F
Rolink
A G
AG
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D001483:Base Sequence; D001853:Bone Marrow / Q000502:physiology / Q000648:ultrastructure; D001854:Bone Marrow Cells; D005260:Female; D005803:Genes, Immunoglobulin; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*
D007135:Immunoglobulin Variable Region; D011498:Protein Precursors; D011947:Receptors, Antigen, B-Cell; D019009:Proto-Oncogene Proteins c-kit