The regulation of Streptococcus mutans glucan-binding protein A expression.
The S. mutans GBP-A is hypothesized to be constitutively expressed and to contribute to the sucrose-dependent colonization of S. mutans. To investigate GBP-A expression, a reporter gene encoding chloramphenicol acetyltransferase (CAT) was placed downstream of the gbpA promoter and CAT activity was measured under conditions that would be associated with the sucrose-dependent colonization of S. mutans. Expression of GBP-A was optimal under anaerobiosis and neutral pH conditions, and correlated with optimal growth. The addition of sucrose to the growth medium did not elevate the expression of GBP-A.
Banas
J A
JA
Department of Microbiology, Immunology, and Molecular Genetics, Albany Medical College, NY 12208, USA. Jeff_Banas@CCGATEWAY.AMC.EDU
Potvin
H C
HC
Singh
R N
RN
D002352:Carrier Proteins / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D037102:Lectins; D013295:Streptococcus mutans / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology
D002352:Carrier Proteins; D037102:Lectins; C037937:glucan-binding proteins; D013395:Sucrose