Measurement of cryoprotective solvent penetration into intact organ tissues using high-field NMR microimaging.
Existing methods are not able to monitor accurately the penetration of cryoprotective solvents (CPS) into intact tissues. In this study, NMR imaging is shown to be a noninvasive nondestructive way to measure penetration rates and effective diffusion coefficients of Me2SO into samples of rat kidney and rat liver tissues. This new method is unique in that the measurements obtained are not averaged over the entire tissue volume but may be made at any site in the tissue. Measurements of penetration rates yield values which are similar to literature values, and the effective diffusion coefficients fall within the expected range. The images also suggest an explanation of why CPSs fail to completely protect organs from freezing damage.
Isbell
S A
SA
Perceptual and Motor Skills, Missoula, Montana 59807, USA.
Fyfe
C A
CA
Ammons
R L
RL
Pearson
B
B
D000818:Animals; D001808:Blood Vessels / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000493:pharmacokinetics*; D004058:Diffusion; D004121:Dimethyl Sulfoxide / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007668:Kidney / Q000033:anatomy & histology / Q000098:blood supply / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000033:anatomy & histology / Q000098:blood supply / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D017711:Nonlinear Dynamics; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D012997:Solvents; D014018:Tissue Distribution
D003451:Cryoprotective Agents; D012997:Solvents; D004121:Dimethyl Sulfoxide