title: Administration of antioxidant vitamins does not alter plasma fibrinolytic activity in subjects with central obesity.
abstract: In vitro studies suggest that oxidized low density lipoprotein inhibits fibrinolysis by stimulating the production of plasminogen activator inhibitor -1 (PAI). We assessed the effects of dietary antioxidant vitamins for four weeks on three indices of copper mediated oxidation of very low and low density lipoproteins (VLDL+LDL) and plasma fibrinolytic activities in 15 male subjects with central obesity, a condition associated with increased PAI activity. Vitamin administration resulted in a decrease in production of thiobarbituric acid reactive substances from 29.3 +/- 3.9 to 13.6 +/- 3.5 nmoles/mg VLDL + LDL protein (mean +/- SE, p <0.003), an increase in the lag phase of conjugated diene formation from 94.8 +/- 5.5 to 225.0 +/- 31.9 min (p <0.001) and an increase in reactivity of lysine residues from 73.6% +/- 4.8% to 86.8% +/- 3.6% (p <0.034) demonstrating a reduction in the susceptibility of the lipoproteins to oxidation. However, antioxidant vitamins had no effect on plasma PAI activity, PAI antigen, tissue-type plasminogen activator activity and antigen, fibrinogen and fibrin degradation products. These results do not support the hypothesis that lipoprotein oxidation is a significant cause of impaired fibrinolysis in men with central obesity.
authors.affiliation: Department of Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick 08903-0019, USA.
authors.forename: V A
authors.initials: VA
authors.lastname: Rifici
authors.forename: S H
authors.initials: SH
authors.lastname: Schneider
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: A K
authors.initials: AK
authors.lastname: Khachadurian
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D005342:Fibrinolysis / Q000187:drug effects*; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D009765:Obesity / Q000503:physiopathology*; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000032:analysis; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D017395:Plasminogen Activator Inhibitor 1; D014815:Vitamins; D014302:Trinitrobenzenesulfonic Acid; D010959:Tissue Plasminogen Activator; D001205:Ascorbic Acid