Involvement of catecholamines and glucocorticoids in ethanol-induced suppression of splenic natural killer cell activity in a mouse model for binge drinking.
Ethanol (EtOH) suppresses splenic natural killer (NK) cell activity in a mouse binge drinking model. Direct effects of EtOH and its metabolites are not the major cause of this suppression. Also, catecholamines do not completely explain this suppression. This implicates the involvement of other neuroendocrine mediators in this suppression. Previous studies in this laboratory have shown that RU 486 at a dosage of 100 mg/kg did not affect EtOH-induced suppression of NK cell activity. However, in the present study, RU 486 at a dosage of 200 mg/kg partially blocked the suppression of NK cell activity induced by EtOH. Moreover, corticosterone at levels expected in the free (unbound) form in EtOH-treated mice decreased NK cell activity in vitro. Nadolol in combination with RU 486 blocked the suppression of NK cell activity in EtOH-treated mice. Although there were reasons to suspect that EtOH-induced changes in the levels of growth hormone or prolactin might also contribute to the suppression of NK cell activity; evidence obtained herein did not indicate such involvement. Thus, glucocorticoids and catecholamines seem to be involved in EtOH-induced suppression of NK cell activity. Together, with the direct effects of EtOH, these neuroendocrine mediators seem to be sufficient to explain all of the suppression of NK cell activity caused by EtOH.
Wu
W J
WJ
Department of Biological Sciences, Mississippi State University, Mississippi, USA.
Pruett
S B
SB
D000435:Alcoholic Intoxication / Q000276:immunology*; D000818:Animals; D002395:Catecholamines / Q000502:physiology*; D003345:Corticosterone / Q000502:physiology; D003864:Depression, Chemical; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D005938:Glucocorticoids / Q000502:physiology*; D013006:Growth Hormone / Q000502:physiology; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011388:Prolactin / Q000502:physiology; D013154:Spleen / Q000187:drug effects* / Q000276:immunology
D002395:Catecholamines; D005938:Glucocorticoids; D000431:Ethanol; D011388:Prolactin; D013006:Growth Hormone; D003345:Corticosterone