PCR-based random mutagenesis using manganese and reduced dNTP concentration.
Lin-Goerke
J L
JL
SmithKline Beecham Pharmaceuticals, Collegeville, PA 19426-0989, USA.
Robbins
D J
DJ
Burczak
J D
JD
D002460:Cell Line; D005091:Exons; D005697:Galactosides; D006801:Humans; D007211:Indoles; D007544:Isopropyl Thiogalactoside; D008345:Manganese / Q000494:pharmacology*; D009074:Melanocyte-Stimulating Hormones / Q000235:genetics*; D016296:Mutagenesis; D009711:Nucleotides / Q000378:metabolism*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D005697:Galactosides; D007211:Indoles; D009711:Nucleotides; D007544:Isopropyl Thiogalactoside; D008345:Manganese; D009074:Melanocyte-Stimulating Hormones; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside