The carboxyl-terminal 120-residue polypeptide of infectious bronchitis virus nucleocapsid induces cytotoxic T lymphocytes and protects chickens from acute infection.
Specific cytotoxic T-lymphocyte (CTL) responses to nucleocapsid of infectious bronchitis virus (IBV) were identified by using target cells infected with a Semliki Forest virus (SFV) vector. Effector cells for CTL assays were collected from chickens infected with the Gray strain of IBV or inoculated with a DNA plasmid encoding nucleocapsid proteins. IBV-specific CTL epitopes were mapped within the carboxyl-terminal 120 amino acids of the nucleocapsid protein. CTL lysis of target cells infected with SFV encoding nucleocapsid was major histocompatibility complex restricted and mediated by CD8+ T cells. In addition, splenic T cells collected from chickens inoculated in the breast muscle with a DNA plasmid encoding this CTL epitope(s) recognized target cells infected with wild-type virus or an SFV vector encoding nucleocapsid proteins. CTL activity of splenic T cells collected from chicks immunized with a DNA plasmid encoding CTL epitopes was cross-reactive, in that lysis of target cells infected with serologically distinct strains of IBV was dose responsive in a manner similar to that for lysis of target cells infected with the homologous strain of IBV. Furthermore, chickens immunized with a DNA plasmid encoding a CTL epitope(s) were protected from acute viral infection.
Seo
S H
SH
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
Wang
L
L
Smith
R
R
Collisson
E W
EW
D000818:Animals; D002645:Chickens; D018352:Coronavirus Infections / Q000276:immunology* / Q000517:prevention & control; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D005822:Genetic Vectors; D001351:Infectious bronchitis virus / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D007668:Kidney / Q000821:virology; D008168:Lung / Q000821:virology; D019251:Nucleocapsid / Q000096:biosynthesis* / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D010957:Plasmids; D012672:Semliki forest virus / Q000235:genetics; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology; D019444:Vaccines, DNA; D014764:Viral Proteins / Q000096:biosynthesis; D014765:Viral Vaccines; D014779:Virus Replication
D010446:Peptide Fragments; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines