title: Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes.
abstract: From a genomic library of Sulfolobus solfataricus DSM1617 we have isolated and identified the purEK locus. Two open reading frames are identified as homologs of the purE and purK purine biosynthetic genes in Escherichia coli. The C-terminus of purE overlaps with the N-terminus of purK. When either of the genes is expressed from an E. coli promoter they can complement the corresponding purE and purK mutations in E. coli. PurE seems to be more closely related to eubacteria than to other archaea and to eukaryotes. Also the purK gene, which has not yet been found in other archaea, is more closely related to eubacteria than to eukaryotes.
authors.affiliation: Department of Biological Chemistry, University of Copenhagen, Denmark.
authors.forename: I S
authors.initials: IS
authors.lastname: Sørensen
authors.forename: G
authors.initials: G
authors.lastname: Dandanell
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D011687:Purines / Q000096:biosynthesis*; D016944:Sulfolobus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011687:Purines; D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase; C075015:purK protein, E coli; C030985:purine