Increase in immunoreactivity for endothelin-1 in blood vessels of rat liver metastases: experimental sarcoma and carcinoma.
Using electron immunocytochemistry, blood vessels in the normal rat liver and in 2 different animal models of liver metastases: (1) Hooded Lister rat with MC28 tumour, a sarcoma, and (2) nude rat with HT29 tumour, a carcinoma, were investigated for the presence of endothelin-1. In the normal livers, small subpopulations of vascular endothelial cells displayed discrete immunoreactivity for endothelin-1. In the livers with malignant tumours, there was a substantial increase in endothelin-1-immunoreactive endothelial cells in vessels located at the tumour periphery. In the controls, antibody to endothelin-1 also labelled sporadically some fibroblast/fibroblast-like cells associated with the blood vessels. In contrast, intense immunoreactivity for endothelin-1 was frequently associated with the tumour cells and/or fibroblast cells in both types of tumour examined.
Loesch
A
A
Department of Anatomy and Developmental Biology, University College London, UK.
Turmaine
M
M
Loizidou
M
M
Crowe
R
R
Ashraf
S
S
Taylor
I
I
Burnstock
G
G
D000818:Animals; D002277:Carcinoma / Q000098:blood supply* / Q000737:chemistry; D019332:Endothelin-1 / Q000032:analysis*; D004730:Endothelium, Vascular / Q000737:chemistry; D005347:Fibroblasts / Q000737:chemistry; D008099:Liver / Q000098:blood supply / Q000737:chemistry; D008114:Liver Neoplasms, Experimental / Q000098:blood supply / Q000737:chemistry / Q000556:secondary*; D008297:Male; D016253:Microscopy, Immunoelectron; D051381:Rats; D011919:Rats, Inbred Strains; D011923:Rats, Nude; D012513:Sarcoma, Experimental / Q000098:blood supply* / Q000737:chemistry
D019332:Endothelin-1