Flow cytometric study of blood leucocytes in clinical forms of human schistosomiasis.
The aim of this study was to examine three distinct groups of schistosomiasis patients and to determine whether cell phenotype profiles could be correlated with the different clinical forms of the disease. The data obtained indicate that Schistosoma mansoni infected patients have a lower percentage of CD3+ T cells than do non-infected individuals. Interestingly, infected patients presented more than twice the mean percentage of circulating activated T cells (CD3+HLA-DR+) when compared to the control group. Examination of T lymphocyte subpopulations showed that patients with the severe hepatosplenic form (HS) of the disease had lower levels of both CD8High+ and CD8Low+ cells when compared to the other groups of patients. All infected individuals had a higher percentage of circulating B cells, with an increase in the CD5+ B cell population that was more evident in the HS group. The data presented here are evidence to support a relationship between the hepatosplenic form of the disease, a decrease on the CD8+ cell population and an elevation on CD5+ B cells.
Martins-Filho
O A
OA
Centro de Pesquisas René Rachou, Fundaçõ Oswaldo Cruz-FIOCRUZ, Belo Horizonte, Brazil.
Dutra
W O
WO
Freeman
G L
GL
Silveira
A M
AM
Rabello
A
A
Colley
D G
DG
Prata
A
A
Gazzinelli
G
G
Correa-Oliveira
R
R
Carvalho-Parra
J
J
D000293:Adolescent; D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002648:Child; D005243:Feces / Q000469:parasitology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000145:classification / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD