title: Severe digestive pathology associated with chronic Chagas' disease in Ecuador: report of two cases.
abstract: DNA extracted from peripheral blood of two Ecuadorian patients showing severe digestive pathology was amplified by the polymerase chain reaction using a Trypanosoma cruzi specific oligonucleotide primers derived from the primary sequence of a cDNA encoding for a 24 kDa excretory/secretory protein. The positive PCR results together with the clinical findings confirmed that both patients had a digestive pathology due to Chagas' disease. This pathology could be more frequent than previously described in the chagasic endemic regions of Andean countries.
authors.affiliation: Department of Clinical Investigations, Hospital Vozandes, Quito, Ecuador.
authors.forename: A G
authors.initials: AG
authors.lastname: Guevara
authors.forename: J W
authors.initials: JW
authors.lastname: Eras
authors.forename: M
authors.initials: M
authors.lastname: Recalde
authors.forename: L
authors.initials: L
authors.lastname: Vinueza
authors.forename: P J
authors.initials: PJ
authors.lastname: Cooper
authors.forename: A
authors.initials: A
authors.lastname: Ouaissi
authors.forename: R H
authors.initials: RH
authors.lastname: Guderian
mesh_terms: D000368:Aged; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D014355:Chagas Disease / Q000150:complications* / Q000175:diagnosis; D002908:Chronic Disease; D016054:DNA, Protozoan / Q000097:blood; D004066:Digestive System Diseases / Q000175:diagnosis / Q000209:etiology*; D004484:Ecuador; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014349:Trypanosoma cruzi / Q000235:genetics / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan