title: Residues in the WD repeats of Tup1 required for interaction with alpha2.
abstract: The yeast transcriptional repressor Tup1 contains seven WD repeats which interact with the DNA-binding protein alpha2. We have identified mutations in Tup1 that disrupt this interaction. The positions of the amino acids changed by these mutations are consistent with Tup1 being folded into a seven-bladed propeller like that formed by another WD repeat-containing protein, the beta subunit of the heterotrimeric G protein used in signal transduction. Our results also indicate that the interaction between Tup1 and alpha2 resembles the interaction between Gbeta and G alpha, suggesting that a similar structural interface is formed by WD repeat proteins that are used in both transcriptional regulation and signal transduction.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0414, USA.
authors.forename: K
authors.initials: K
authors.lastname: Komachi
authors.forename: A D
authors.initials: AD
authors.lastname: Johnson
mesh_terms: D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D018398:Homeodomain Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D017354:Point Mutation; D011485:Protein Binding; D017510:Protein Folding; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic / Q000235:genetics; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D018398:Homeodomain Proteins; C062928:MATA2 protein, S cerevisiae; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C066502:TUP1 protein, S cerevisiae; D001224:Aspartic Acid; D014364:Tryptophan