Phosphorylation-directed antibodies in high-flux screens for compounds that modulate signal transduction.
Synthetic tyrosine phosphopeptides were used to generate antibodies that recognize phosphotyrosine in the context of a defined sequence of flanking amino acids. Using phosphopeptide immunogens derived from regulatory or signal-generating motifs, "phosphorylation-directed antibodies" can be targeted to specific growth factor receptors or signal-generating proteins. In his paper, we show how phosphorylation-directed antibodies can be used in a colorimetric, high-throughput screen for drugs that modulate the function of specific growth factor receptors or signal-generating proteins.
Alberta
J A
JA
Harvard Medical School, Boston, MA, USA.
Stiles
C D
CD
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay; D015151:Immunoblotting; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000378:metabolism; D017978:Receptors, Growth Factor / Q000187:drug effects; D015398:Signal Transduction
D000906:Antibodies; D019000:Phosphotyrosine; D018719:Erb-b2 Receptor Tyrosine Kinases; D017978:Receptors, Growth Factor