title: Identification of a cellular receptor for subgroup E avian leukosis virus.
abstract: Genetic studies in chickens and receptor interference experiments have indicated that avian leukosis virus (ALV)-E may utilize a cellular receptor related to the receptor for ALV-B and ALV-D. Recently, we cloned CAR1, a tumor necrosis factor receptor (TNFR)-related protein, that serves as a cellular receptor for ALV-B and ALV-D. To determine whether the cellular receptor for ALV-E is a CAR1-like protein, a cDNA library was made from turkey embryo fibroblasts (TEFs), which are susceptible to ALV-E infection, but not to infection by ALV-B and ALV-D. The cDNA library was screened with a radioactively labeled CAR1 cDNA probe, and clones that hybridized with the probe were isolated. A 2.3-kb cDNA clone was identified that conferred susceptibility to ALV-E infection, but not to ALV-B infection, when expressed in transfected human 293 cells. The functional cDNA clone is predicted to encode a 368 amino acid protein with significant amino acid similarity to CAR1. Like CAR1, the TEF protein is predicted to have two extracellular TNFR-like cysteine-rich domains and a putative death domain similar to those of TNFR I and Fas. Flow cytometric analysis and immunoprecipitation experiments demonstrated specific binding between the TEF CAR1-related protein and an immunoadhesin composed of the surface (SU) envelope protein of subgroup E (RAV-0) virus fused to the constant region of a rabbit immunoglobulin. These two activities of the TEF CAR1-related protein, specific binding to ALV-E SU and permitting entry only of ALV-E, have unambiguously identified this protein as a cellular receptor specific for subgroup E ALV.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: H B
authors.initials: HB
authors.lastname: Adkins
authors.forename: J
authors.initials: J
authors.lastname: Brojatsch
authors.forename: J
authors.initials: J
authors.lastname: Naughton
authors.forename: M M
authors.initials: MM
authors.lastname: Rolls
authors.forename: J M
authors.initials: JM
authors.lastname: Pesola
authors.forename: J A
authors.initials: JA
authors.lastname: Young
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000595:Amino Acid Sequence; D000818:Animals; D001119:Arginase / Q000096:biosynthesis / Q000737:chemistry; D001354:Avian Leukosis Virus / Q000145:classification / Q000502:physiology*; D030161:Avian Proteins; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian; D005347:Fibroblasts; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry; D015723:Gene Library; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry; D008969:Molecular Sequence Data; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry; D011991:Receptors, Virus / Q000096:biosynthesis* / Q000737:chemistry / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014422:Turkeys
chemical_list: D030161:Avian Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; C088745:Tva receptor; D000469:Alkaline Phosphatase; D001119:Arginase