Advancement through mitosis requires rae1 gene function in fission yeast.
Growth of the rae1-1 mutant of Schizosaccharomyces pombe at restrictive temperature results in accumulation of poly(A)+ RNA in the nucleus and a cell cycle arrest at the G2/M boundary. We demonstrate here that rae1 function is required for a process other than mRNA export which is essential for advancement through mitosis. Cells lacking rae1 function arrest with elevated Cdc2p kinase levels at a step before the formation of a mitotic spindle and without separation of the spindle pole bodies. Rae1p was localized to the nuclear periphery, consistent with a role in nucleocytoplasmic trafficking, which could include protein import. We propose a model where rae1 functions in cell cycle progression through trafficking of proteins required for mitosis.
Whalen
W A
WA
Laboratory of Molecular Virology, National Cancer Institute, Bethesda, MD 20892, USA.
Bharathi
A
A
Danielewicz
D
D
Dhar
R
R
D016203:CDC2 Protein Kinase / Q000378:metabolism; D002453:Cell Cycle; D003513:Cycloheximide / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000502:physiology*; D008938:Mitosis; D034521:Nuclear Matrix-Associated Proteins; D029543:Nucleocytoplasmic Transport Proteins; D012333:RNA, Messenger / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins
D005656:Fungal Proteins; D034521:Nuclear Matrix-Associated Proteins; D029543:Nucleocytoplasmic Transport Proteins; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; C092953:rae1 protein, S pombe; D003513:Cycloheximide; D016203:CDC2 Protein Kinase