Acetylcholinesterase (ACE) staining shows the abnormal innervation of a pulled-through rectum in a case of repaired anorectal malformation.
Abnormal innervation of the anorectum was noted in relation to anal incontinence in a case of repaired high-type anorectal malformation (ARM). A ten-year-old boy presented with anal incontinence after reconstructive surgery of ARM with a recto-urethral fistula. An anorectal manometrical examination revealed both an adequate tonus of the anal sphincter muscles and the absence of rectoanal reflex relaxation. And a barium enema showed a narrow region in the rectosigmoid colon, which was similar to that of Hirschsprung's disease (HD). Furthermore, an acetylcholinesterase (ACE) histochemical study of the rectal suction biopsies revealed an increased number of ACE-positive nerve fibers in the lamina propria mucosae and muscularis mucosae of the pulled-through colon. At the same time, however, some ganglia cells were also observed in the submucosa of the affected rectosigmoid colon and these cells could not be found in HD. Although the mechanism by which the abnormally innervated parasympathetic nerve fibers arose in the pulled-through colon remains unclear, this neuronal abnormality is considered to be the cause of anal incontinence in this case.
Shono
T
T
Department of Surgery, Fukuoka Children's Hospital Fukuoka, Japan.
Nagasaki
A
A
Hirose
R
R
Ohgami
H
H
Yakabe
S
S
D000110:Acetylcholinesterase / Q000378:metabolism*; D001006:Anus, Imperforate / Q000473:pathology / Q000601:surgery*; D002648:Child; D005242:Fecal Incontinence / Q000473:pathology / Q000601:surgery*; D006627:Hirschsprung Disease / Q000473:pathology / Q000601:surgery; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology / Q000601:surgery; D008297:Male; D009412:Nerve Fibers / Q000473:pathology; D011183:Postoperative Complications / Q000473:pathology / Q000601:surgery*; D012003:Rectal Fistula / Q000473:pathology / Q000601:surgery; D012007:Rectum / Q000294:innervation* / Q000601:surgery; D014522:Urethral Diseases / Q000473:pathology / Q000601:surgery; D014548:Urinary Fistula / Q000473:pathology / Q000601:surgery
D000110:Acetylcholinesterase