title: Gastric ulcer healing in the rat: kinetics and localisation of de novo procollagen synthesis.
abstract: OBJECTIVE
  To gain further insight into the role of the extracellular matrix during healing of peptic ulcers, sequential changes of procollagen expression were studied over 30 days of ulcer healing.
  
  
  METHODS
  Procollagens alpha 1 (I), alpha 1 (III), and alpha 1 (IV) RNA and their polypeptides were assessed in acetic acid induced rat gastric ulcers by in situ hybridisation and immunohistochemistry.
  
  
  RESULTS
  Three days after ulcer induction, intense hybridisation signals were obtained with all probes, with procollagen alpha 1 (I) showing the highest transcript levels. Procollagen gene expression remained elevated up to day 15, but was reduced to initial low levels on day 30. Immunohistochemical staining documented increased deposition of the three procollagen types parallel to their respective transcript levels, again with type I showing the earliest and the most prominent deposits. The highest procollagen transcript levels were found in the intact submucosa surrounding the ulcer margins, followed by the muscularis propria and the serosa, with the lamina propria exhibiting the lowest transcript levels.
  
  
  CONCLUSIONS
  The procollagens studied are regulated differentially at the transcriptional and post-transcriptional levels. The early onset and long duration of procollagen expression as well as the involvement of all layers of the gastric wall points to their central structural and functional role in gastric ulcer healing.
authors.affiliation: Department of Medicine B, University of Münster, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Shahin
authors.forename: A
authors.initials: A
authors.lastname: Gillessen
authors.forename: T
authors.initials: T
authors.lastname: Pohle
authors.forename: C
authors.initials: C
authors.lastname: Weber
authors.forename: D
authors.initials: D
authors.lastname: Schuppan
authors.forename: H
authors.initials: H
authors.lastname: Herbst
authors.forename: W
authors.initials: W
authors.lastname: Domschke
mesh_terms: D019342:Acetic Acid; D000818:Animals; D005109:Extracellular Matrix / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D011347:Procollagen / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000378:metabolism* / Q000503:physiopathology; D014945:Wound Healing
chemical_list: D011347:Procollagen; D012333:RNA, Messenger; D019342:Acetic Acid