Diminished response to food deprivation of the rat brown adipose tissue mitochondrial uncoupling system with age.
The aim of the present work is to investigate the effect of starvation on brown adipose tissue thermogenic activity with aging. Interscapular brown adipose tissue from female Wistar rats of different ages was used; half of them were fed and the other half were starved for 24 hours. Mitochondria were isolated and mitochondrial protein content, GDP-binding, Cytochrome-c Oxidase activity and uncoupling protein levels were measured. Results show a decrease of all studied parameters, indicating a diminished thermogenic activity with age. The response to starvation is almost the same in all the parameters studied: a general reduction with starvation and a progressive disappearance of this response to starvation with aging. On the whole, these results would indicate a deficient regulation of brown adipose tissue thermogenic activity in old animals, as it happens in other animal models with an alterated thermogenesis.
Garcia-Palmer
F J
FJ
Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Pericas
J
J
Matamala
J C
JC
Puigserver
P
P
Bonet
M L
ML
Palou
A
A
Gianotti
M
M
D000273:Adipose Tissue / Q000502:physiology*; D002001:Adipose Tissue, Brown / Q000502:physiology; D000367:Age Factors; D000818:Animals; D001833:Body Temperature Regulation / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D005508:Food Deprivation; D006153:Guanosine Diphosphate / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry / Q000502:physiology*; D009929:Organ Size / Q000502:physiology; D011506:Proteins / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013217:Starvation
D011506:Proteins; D006153:Guanosine Diphosphate; D003576:Electron Transport Complex IV