Characterization of a linear DNA plasmid from the filamentous fungal plant pathogen Glomerella musae [Anamorph: Colltotrichum musae (Berk. & Curt.) Arx.].
A 7.4-kilobase (kb) DNA plasmid was isolated from Glomerella musae isolate 927 and designated pGML1. Exonuclease treatments indicated that pGML1 was a linear plasmid with blocked 5' termini. Cell-fractionation experiments combined with sequence-specific PCR amplification revealed that pGML1 resided in mitochondria. The pGML1 plasmid hybridized to cesium chloride-fractionated nuclear DNA but not to A + T-rich mitochondrial DNA. An internal 7.0-kb section of pGML1 was cloned and did not hybridize with either nuclear or mitochondrial DNA from G. musae. Sequence analysis revealed identical terminal inverted repeats (TIR) of 520 bp at the ends of the cloned 7.0-kb section of pGML1. The occurrence of pGML1 did not correspond with the pathogenicity of G. musae on banana fruit. Four additional isolates of G. musae possessed extrachromosomal DNA fragments similar in size and sequence to pGML1.
Freeman
S
S
Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan 50250, Israel.
Redman
R S
RS
Grantham
G
G
Rodriguez
R J
RJ
D001203:Ascomycota / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D003001:Cloning, Molecular; D010944:Plants / Q000382:microbiology; D010957:Plasmids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013045:Species Specificity