title: Lipochitooligosaccharide-induced tobacco cells release a peptide as mediator of the glycolipid signal.
abstract: Lipochitooligosaccharides (LCOs) are plant growth regulators that promote at subfemtomolar concentrations cell division in tobacco protoplasts. In response to LCO treatment, tobacco cells release a second growth factor that fully mediates the growth-promoting activities of the initial extracellular LCO stimulus. This diffusible growth factor was isolated from the protoplasts' culture filtrate and shown to be a peptide. We report that the LCO-induced mitogen released by tobacco cells and a synthetic heptadecapeptide derived from region 2 of the tobacco homolog of the early nodulin gene ENOD40 are antigenically related and qualitatively indistinguishable in their ability to stimulate cell division.
authors.affiliation: Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Cologne, Germany. John@mpiz-koeln.mpg.de
authors.forename: M
authors.initials: M
authors.lastname: John
authors.forename: J
authors.initials: J
authors.lastname: Schmidt
authors.forename: R
authors.initials: R
authors.lastname: Walden
authors.forename: I
authors.initials: I
authors.lastname: Czaja
authors.forename: M
authors.initials: M
authors.lastname: Dülz
authors.forename: J
authors.initials: J
authors.lastname: Schell
authors.forename: H
authors.initials: H
authors.lastname: Röhrig
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary; D006017:Glycolipids / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008934:Mitogens / Q000378:metabolism; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics; D010947:Plants, Toxic; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D006017:Glycolipids; D008070:Lipopolysaccharides; D008934:Mitogens; D010937:Plant Growth Regulators; D010940:Plant Proteins; C023023:lipid-linked oligosaccharides