Determination of 8-hydroxy-deoxyguanosine formation in rat organs: assessment of paraquat-evoked oxidative DNA damage.
Paraquat has previously been shown to cause the oxidative damage to DNA in variety of cells and tissues. However, although paraquat-evoked strand breaks has been extensively studied to assess the DNA damage, the effect of paraquat on base modifications, another marker for the oxidative damage, has not yet been investigated. To further characterize paraquat-evoked DNA damage, the effect of paraquat on 8-hydroxy-deoxyguanosine (8-OH-dG) formation in various rat organs was examined. Paraquat markedly increased 8-OH-dG contents in various organs, particularly in brain, lung and heart, and this increase reached the maximum levels at 5 days after the drug administration. In contrast, the formation of 8-hydroxy-guanosine (8-OH-G), a marker for the oxidative damage to RNA, was not significantly affected by paraquat. These results indicate that paraquat causes base modifications as well as strand breaks as a consequence of the oxidative damage to DNA.
Tokunaga
I
I
Department of Legal Medicine, Tokushima University School of Medicine, Japan.
Kubo
S
S
Mikasa
H
H
Suzuki
Y
Y
Morita
K
K
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D006151:Guanosine / Q000031:analogs & derivatives / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D010084:Oxidation-Reduction; D010269:Paraquat / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D006151:Guanosine; C046215:8-hydroxyguanosine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D010269:Paraquat