title: Elevated levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis.
abstract: The presence of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) activities was determined in milk and serum of cows with naturally occurring coliform mastitis (CFM). TNF-alpha was detected in the sera from 26 of 32 cows with CFM. TNF-alpha levels were higher in the sera than in the milk. IL-6 was high in the sera of surviving CFM animals, but was low in animals that died and in healthy controls. Furthermore, the mean level of IL-6 was 20-fold higher in the milk than in the sera of mastitic cows. The level of IL-6 in the serum was correlated to that in the milk in individual animals. The presence of IL-6 and TNF-alpha in the sera appears to relate to severe clinical condition of CFM, in the milk whereas they may play a role in generating inflammation of the mammary gland.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakajima
authors.forename: O
authors.initials: O
authors.lastname: Mikami
authors.forename: M
authors.initials: M
authors.lastname: Yoshioka
authors.forename: Y
authors.initials: Y
authors.lastname: Motoi
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Fuse
authors.forename: K
authors.initials: K
authors.lastname: Nakano
authors.forename: K
authors.initials: K
authors.lastname: Yasukawa
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000378:metabolism; D004754:Enterobacter / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D004926:Escherichia coli / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000097:blood / Q000378:metabolism / Q000662:veterinary; D005260:Female; D015850:Interleukin-6 / Q000032:analysis* / Q000097:blood*; D007709:Klebsiella / Q000302:isolation & purification; D007710:Klebsiella Infections / Q000097:blood / Q000378:metabolism / Q000662:veterinary; D008414:Mastitis, Bovine / Q000097:blood / Q000378:metabolism* / Q000382:microbiology; D008892:Milk / Q000737:chemistry* / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha