In vivo and in vitro activation and expansion of gammadelta T cells during Listeria monocytogenes infection in humans.
Serial flow cytometry analyses of peripheral blood mononuclear cells obtained from 8 patients infected with Listeria monocytogenes showed a higher percentage (P < 0.01) of gammadelta T cells (median, 11.7; range, 3.7 to 35.3) than did 16 age-matched uninfected controls (1.7, 0.4 to 13). Most in vivo-expanded gammadelta T cells expressed the Vgamma9 and Vdelta2 gene products and displayed a memory phenotype (CD45RO[high]), and patients' gammadelta T cells expressed significantly more (P < 0.01) activation marker HLA-DR than did controls (19.8% [median] and 0.9 to 87.6% [range] versus 2.3% and 0 to 4.7%, respectively). When peripheral blood mononuclear cells from normal donors were cultured in vitro with heat-killed Listeria cells, analysis of CD25 and HLA-DR expression on gammadelta and alphabeta T cells indicated that a high percentage of gammadelta T cells was activated early compared to alphabeta T cells. In addition, depletion of gammadelta T cells before culture abrogated the early lymphocyte proliferative response induced by the pathogen. Taken together, these results argue for the involvement of gammadelta T cells during L. monocytogenes infection in humans.
Jouen-Beades
F
F
Groupe de Recherche en Immunopathologie, Institut Fédératif de Recherche Multidisciplinaire sur les Peptides, Hôpital Charles Nicolle, Rouen, France.
Paris
E
E
Dieulois
C
C
Lemeland
J F
JF
Barre-Dezelus
V
V
Marret
S
S
Humbert
G
G
Leroy
J
J
Tron
F
F
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006684:HLA-DR Antigens; D006801:Humans; D007111:Immunity, Cellular; D007156:Immunologic Memory; D007223:Infant; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens; D008088:Listeriosis / Q000276:immunology*; D008137:Longitudinal Studies; D008213:Lymphocyte Activation; D008297:Male; D010641:Phenotype; D011247:Pregnancy; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D015415:Biomarkers; D006684:HLA-DR Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2; D017493:Leukocyte Common Antigens