title: Quantification of bovine albumin fraction V in cellular antigens of antileptospirosical Cuban vaccine vaxSpiral.
abstract: A solid phase immunoradiometric assay (IRMA) for quantification of bovine albumin fraction V (Bov.Alb.FV) in antileptospirosical Cuban vaccine vaxSpiral is described in the present work. Anti-Bov.Alb.FV IgG raised against rabbit purified by affinity chromatography was used as first antibody. Anti-rabbit IgG labeled by Chloromine-T reaction was used as a tracer and the method has demonstrated to be sensitive with high intra- and inter-assay reproducibility. Eight lots of vaccinal antigens were evaluated and in all of the cases, the bovine albumin fraction V concentration was lower than 1 microgram/mL, as the World Health Organization (WHO) establishes. This IRMA is a simple and sensitive assay and could be used as control method for all human vaccines that use Bov.Alb.FV in their production process, even cellular vaccines.
authors.affiliation: Finlay Institute, La Habana, Cuba.
authors.forename: T
authors.initials: T
authors.lastname: Valdés González
authors.forename: M
authors.initials: M
authors.lastname: González Pérez
authors.forename: M
authors.initials: M
authors.lastname: González González
authors.forename: R A
authors.initials: RA
authors.lastname: Cabrera Arias
authors.forename: E
authors.initials: E
authors.lastname: Muñoz Carnago
authors.forename: M
authors.initials: M
authors.lastname: Lastre González
authors.forename: G
authors.initials: G
authors.lastname: Sierra González
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001428:Bacterial Vaccines / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D015592:Immunoradiometric Assay; D007919:Leptospira / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000032:analysis*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine