title: A simple PCR-based procedure for plague diagnosis.
abstract: Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals.
authors.affiliation: Centro de Pesquisas Aggeu Magalhaes, FIOCRUZ/MS, Cidade Universitária, Recife, PE, Brazil.
authors.forename: N C
authors.initials: NC
authors.lastname: Leal
authors.forename: F G
authors.initials: FG
authors.lastname: Abath
authors.forename: L C
authors.initials: LC
authors.lastname: Alves
authors.forename: A M
authors.initials: AM
authors.lastname: de Almeida
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D051379:Mice; D010930:Plague / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial