Immunoaffinity column cleanup procedure for analysis of ivermectin in swine liver.
A simple and sensitive method for the analysis of ivermectin (22,23-dihydroavermectin B1) in swine liver based on immunoaffinity column cleanup is described. The immunosorbent was prepared by coupling polyclonal anti-ivermectin antibodies to carbonyl diimidazole-activated Sepharose CL-4B. After extraction with methanol, ivermectin was cleaned up on an immunoaffinity column, and determined by reversed-phase liquid chromatography with UV absorbance detection at 245 nm. Recoveries of ivermectin from fortified samples of 5-10 micrograms kg-1 levels ranged 85-102%, with coefficients of variation of 6-12%. The limit of detection was 2 micrograms kg-1 in a 5-g samples.
Li
J S
JS
Department of Veterinary Pharmacology, Pharmacy and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
Li
X W
XW
Hu
H B
HB
D000818:Animals; D000977:Antiparasitic Agents / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D007106:Immune Sera / Q000737:chemistry / Q000276:immunology; D007164:Immunosorbents / Q000737:chemistry / Q000276:immunology; D007559:Ivermectin / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D008099:Liver / Q000737:chemistry*; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D000977:Antiparasitic Agents; D007106:Immune Sera; D007164:Immunosorbents; D007559:Ivermectin