SNAP-23 is located in the basolateral plasma membrane of rat pancreatic acinar cells.
The SNARE hypothesis proposes that specificity of exocytosis is regulated by the appropriate interactions between the vesicle (v-) SNARE and the target membrane (t-) SNAREs. We show here that pancreatic acinar cells express the SNAP-25 t-SNARE homolog SNAP-23, and find that this t-SNARE is most highly concentrated on the basolateral plasma membrane while being expressed below detectable levels in endocrine islets within the same tissue. This is the first localization of SNAP-23 within a polarized tissue and suggests that this t-SNAREs may interact with syntaxin-4 to mediate basolateral secretion.
Gaisano
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HY
Department of Medicine, University of Toronto, The Toronto Hospital, Ont., Canada.
Sheu
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L
Wong
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PP
Klip
A
A
Trimble
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WS
D016475:3T3 Cells; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000648:ultrastructure; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D007425:Intracellular Membranes / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D018613:Microscopy, Confocal; D010179:Pancreas / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley
D002352:Carrier Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C100493:SNAP23 protein, human; C107017:Snap23 protein, mouse