title: Cloning and characterization of TRAIL-R3, a novel member of the emerging TRAIL receptor family.
abstract: TRAIL-R3, a new member of the TRAIL receptor family, has been cloned and characterized. TRAIL-R3 encodes a 299 amino acid protein with 58 and 54% overall identity to TRAIL-R1 and -R2, respectively. Transient expression and quantitative binding studies show TRAIL-R3 to be a plasma membrane-bound protein capable of high affinity interaction with the TRAIL ligand. The TRAIL-R3 gene maps to human chromosome 8p22-21, clustered with the genes encoding two other TRAIL receptors. In contrast to TRAIL-R1 and -R2, this receptor shows restricted expression, with transcripts detectable only in peripheral blood lymphocytes and spleen. The structure of TRAIL-R3 is unique when compared to the other TRAIL receptors in that it lacks a cytoplasmic domain and appears to be glycosyl-phosphatidylinositol-linked. Moreover, unlike TRAIL-R1 and -R2, in a transient overexpression system TRAIL-R3 does not induce apoptosis.
authors.affiliation: Department of Biochemistry and the Department of Molecular Biology, Immunex Corporation, Seattle, Washington 98101, USA. mdegliesposti@immunex.com
authors.forename: M A
authors.initials: MA
authors.lastname: Degli-Esposti
authors.forename: P J
authors.initials: PJ
authors.lastname: Smolak
authors.forename: H
authors.initials: H
authors.lastname: Walczak
authors.forename: J
authors.initials: J
authors.lastname: Waugh
authors.forename: C P
authors.initials: CP
authors.lastname: Huang
authors.forename: R F
authors.initials: RF
authors.lastname: DuBose
authors.forename: R G
authors.initials: RG
authors.lastname: Goodwin
authors.forename: C A
authors.initials: CA
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D003001:Cloning, Molecular; D058851:GPI-Linked Proteins; D005786:Gene Expression Regulation; D017261:Glycosylphosphatidylinositols; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics; D019169:Jurkat Cells; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D053221:TNF-Related Apoptosis-Inducing Ligand; D053319:Tumor Necrosis Factor Decoy Receptors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D051017:Apoptosis Regulatory Proteins; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D007141:Immunoglobulin Fc Fragments; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D011993:Recombinant Fusion Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C108661:TNFRSF10C protein, human; C505653:TNFSF10 protein, human; D053319:Tumor Necrosis Factor Decoy Receptors; D014409:Tumor Necrosis Factor-alpha