Differential mode of TBP utilization in transcription of the tRNA[Ser]Sec gene and TATA-less class III genes.
The Xenopus laevis selenocysteine tRNA[Ser]Sec gene utilizes the TATA box binding protein (TBP) for its transcription in a manner more like TATA-dependent class II genes than TATA-less class III tRNA genes, even though this gene is transcribed by RNA polymerase III (Pol III). Addition of TBP increased in vitro transcription of the tRNA[Ser]Sec gene and a RNA polymerase II-(Pol II-) dependent template, while it decreased TATA-independent tRNA(Met) gene transcription, in a dose-dependent manner. Addition of wild-type TBP, truncated TBP containing the highly conserved COOH-terminal domain or a mutant TBP defective in TATA-independent Pol III transcription to TBP-depleted extracts restored tRNA[Ser]Sec transcription, while addition of a mutant TBP defective in Pol II transcription did not. These studies provide evidence that common surfaces of TBP may be used in transcription from TATA-dependent promoters of the tRNA[Ser]Sec gene and class II genes. Further, we show that distinct chromatographic fractions of TBP complexes are required for tRNA[Ser]Sec gene transcription and TATA-less class III gene transcription.
Park
J M
JM
Laboratory of Molecular Genetics, Institute for Molecular Biology and Genetics, Seoul National University, South Korea.
Lee
J Y
JY
Hatfield
D L
DL
Lee
B J
BJ
D000818:Animals; D001667:Binding, Competitive; D002457:Cell Extracts; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D009154:Mutation; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics*; D012362:RNA, Transfer, Ser / Q000235:genetics; D016385:TATA Box; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014981:Xenopus
D002457:Cell Extracts; D004268:DNA-Binding Proteins; D012362:RNA, Transfer, Ser; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; D012343:RNA, Transfer; D012319:RNA Polymerase II; D012320:RNA Polymerase III