title: Effect of growth factors on epithelial restitution of human colonic mucosa in vitro.
abstract: BACKGROUND
  Epithelial restitution enables resurfacing of epithelial discontinuities by enterocyte migration. This study investigated the effect of basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF-1), and epidermal growth factor (EGF) on restitution of human colonic mucosa in vitro.
  
  
  METHODS
  After base-line incubation human colonic mucosal strips, mounted in Ussing chambers, were luminally exposed to 0.5 mM sodium deoxycholate (NaDOC) for 10 min. Thereafter tissues were incubated with buffer alone or luminal buffer containing various concentrations of bFGF, IGF-1, and EGF for 3 h. Resistance (R) was calculated from potential difference (PD) and short-circuit current (Isc). All tissues were processed for light microscopy. Extent of damage was measured by morphometry.
  
  
  RESULTS
  Luminal 0.5 mM NaDOC for 10 min caused R to drop by 43% (n = 4; P < 0.05). Compared with controls 50 ng/ml EGF induced an approximately 30% R increase until the end of the experiments (P < 0.05, n = 4, paired). Ten minutes after injury 50.2 +/- 4% of the mucosa was damaged (n = 6), and after 3 h damage was significantly reduced by EGF (17.2 +/- 3% versus 31.7 +/- 4%, 50 ng/ml EGF versus controls) (P < 0.05, n = 6 per group). Histology showed that EGF stimulated enterocyte migration over the basal lamina. Various doses of bFGF and IGF-1 did not impair restitution when compared with controls.
  
  
  CONCLUSIONS
  In contrast to bFGF and IGF-1, EGF was shown to promote epithelial restitution of human colonic mucosa in vitro.
authors.affiliation: University Clinic of Surgery, University of Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Riegler
authors.forename: R
authors.initials: R
authors.lastname: Sedivy
authors.forename: T
authors.initials: T
authors.lastname: Sogukoglu
authors.forename: E
authors.initials: E
authors.lastname: Cosentini
authors.forename: G
authors.initials: G
authors.lastname: Bischof
authors.forename: B
authors.initials: B
authors.lastname: Teleky
authors.forename: W
authors.initials: W
authors.lastname: Feil
authors.forename: R
authors.initials: R
authors.lastname: Schiessel
authors.forename: G
authors.initials: G
authors.lastname: Hamilton
authors.forename: E
authors.initials: E
authors.lastname: Wenzl
mesh_terms: D003106:Colon / Q000187:drug effects* / Q000473:pathology; D004594:Electrophysiology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004848:Epithelium; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D018709:Statistics, Nonparametric
chemical_list: D006133:Growth Substances; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I