A preliminary assessment of the recombinant antigen PLA2 in the diagnosis of human dirofilariosis.
Two recombinant antigens (P22U and PLA2), cloned in a L4 Dirofilaria immitis cDNA library, were analyzed by Western-blot and ELISA to investigate their characteristics for the diagnosis of human dirofilariosis. P22U seems related to a Di22 native antigen useful for the diagnosis of pulmonary dirofilariosis, but it is unspecifically recognized by sera from patients with different parasitic and non parasitic pulmonary diseases. PLA2 is not related to Di22 but specifically reacts in Western-Blot and ELISA with sera from patients with subcutaneous dirofilariosis.
Vieira
C
C
Laboratorio de Parasitología, Universidad de Salamanca, Spain.
Muro
A
A
Cordero
M
M
Simón
F
F
D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D004184:Dirofilariasis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008174:Lung Diseases, Parasitic / Q000175:diagnosis*; D012698:Serologic Tests
D000947:Antigens, Helminth