title: Lac repressor genetic map in real space.
abstract: Here, we present a graphic display of the phenotypes of more than 4000 single amino acid substitution mutations on the three-dimensional structure of the lac repressor tetramer bound to DNA. The genetic data and the X-ray diffraction studies contribute to define an allosteric mechanism and yield a visual demonstration of the importance of core or buried residues in protein structure.
authors.affiliation: Department of Chemistry, University of Pennsylvania, Philadelphia, USA.
authors.forename: H C
authors.initials: HC
authors.lastname: Pace
authors.forename: M A
authors.initials: MA
authors.lastname: Kercher
authors.forename: P
authors.initials: P
authors.lastname: Lu
authors.forename: P
authors.initials: P
authors.lastname: Markiewicz
authors.forename: J H
authors.initials: JH
authors.lastname: Miller
authors.forename: G
authors.initials: G
authors.lastname: Chang
authors.forename: M
authors.initials: M
authors.lastname: Lewis
mesh_terms: D000495:Allosteric Site; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D029968:Escherichia coli Proteins; D056968:Lac Repressors; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D056968:Lac Repressors; C539064:LacI protein, E coli; D012097:Repressor Proteins