Effect of luteinizing hormone alpha-subunit on IL-2 and sIL-2R in vitro secretions from human peripheral blood mononuclear cells.
The effect of luteinizing hormone alpha-subunit (LH alpha-SU) in concentrations of 50.0, 5.0, 0.5, and 0.05 mIU/ml on the release of interleukin-2 (IL-2) and soluble interleukin-2 receptor (sIL-2R) secretions in vitro from resting or phytohaemagglutinin (PHA) activated human peripheral blood mononuclear cells (PBMC) was studied. Both interleukins were measured in supernatants of cultured cells by quantitative sandwich enzyme immunoassay method (ELISA). LH alpha-SU in the concentrations tested did not influence the secretion of IL-2 (p > 0.05) or even depress the release of sIL-2R (50.0, 5.0, 0.5 mIU/ml; p < 0.001) into supernatants of PBMC cultures.
Komorowski
J
J
Institute of Endocrinology, Medical University of Lódź, Poland.
Gradowski
G
G
Stepień
H
H
D000328:Adult; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D007986:Luteinizing Hormone / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000378:metabolism*
D007376:Interleukin-2; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2; D007986:Luteinizing Hormone