Localized 31P MR spectroscopy of the transplanted human kidney in situ shows altered metabolism in rejection and acute tubular necrosis.
The purpose of this study was to investigate the function of transplant kidneys in situ, and to detect pathologic changes, using volume-selective phosphorous NMR spectroscopy (31P MRS). Localized 31P MR spectra were obtained from 37 patients using a whole-body MR scanner with a combination of surface coils, adiabatic excitation pulses, and a modified image-selected in vivo spectroscopy (ISIS) sequence. Seventeen patients with pathologic changes after renal transplant were compared with a control group of 20 patients with no evidence of transplant dysfunction. The transplant kidneys with rejection reaction showed higher ratios of inorganic phosphate (P2i) to adenosine triphosphate-alpha (ATP-alpha) than the normal control group (.4 +/- .16 compared with .22 +/- .11, P = .01) and reduced pH. The spectra of transplant kidneys with tubular necrosis had lower phosphomonoester (PME)/phosphodiester (PDE) ratios than the control group (.65 +/- .35 compared with .96 +/- .5, P = .04). The pathologies of rejection and tubular necrosis could be differentiated from each other by pH (6.93 +/- .1 in rejection versus 7.14 +/- .19 in tubular necrosis, P = .04). Preliminary results indicate that localized image-guided 31P MR spectroscopy of transplant kidneys in situ can detect rejection reactions and acute tubular necrosis noninvasively, providing an incentive for further research.
Heindel
W
W
Department of Diagnostic Radiology, University of Cologne, Köln (Lindenthal), Federal Republic of Germany.
Kugel
H
H
Wenzel
F
F
Stippel
D
D
Schmidt
R
R
Lackner
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D003937:Diagnosis, Differential; D005260:Female; D006084:Graft Rejection / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D016030:Kidney Transplantation / Q000502:physiology*; D007683:Kidney Tubular Necrosis, Acute / Q000473:pathology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate