title: Biochemical characterization, molecular cloning and localization of a putative odorant-binding protein in the honey bee Apis mellifera L. (Hymenoptera : Apidea).
abstract: A honey bee antennal water-soluble protein, APS2, was purified and characterized as the first Hymenoptera putative odorant-binding protein. Comparison of its measured Mr (13695.2+/-1.6) to that of the corresponding cDNA clone shows it does not undergo any post-translational modification other than a 19-residue signal peptide cleavage and formation of three disulfide bridges. These biochemical features are close to those of Lepidoptera odorant-binding proteins. In situ hybridization experiments demonstrated its specific expression in olfactory areas. Based on its higher expression in the worker than in the drone, ASP2 might be more involved in general odorant than in sex pheromone detection.
authors.affiliation: Neurobiologie Expérimentale et Théorie des Systèmes complexes, CNRS UPR 9081, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Danty
authors.forename: C
authors.initials: C
authors.lastname: Michard-Vanhée
authors.forename: J C
authors.initials: JC
authors.lastname: Huet
authors.forename: E
authors.initials: E
authors.lastname: Genecque
authors.forename: J C
authors.initials: JC
authors.lastname: Pernollet
authors.forename: C
authors.initials: C
authors.lastname: Masson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000737:chemistry* / Q000502:physiology; D001522:Behavior, Animal; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007915:Lepidoptera / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D012679:Sense Organs / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: C108516:ASP2 protein, Apis mellifera; D002352:Carrier Proteins; D019476:Insect Proteins