title: Survival of a bacterioferritin deletion mutant of Brucella melitensis 16M in human monocyte-derived macrophages.
abstract: A bacterioferritin (BFR) deletion mutant of Brucella melitensis 16M was generated by gene replacement. The deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pBBR-bfr. The survival and growth of the mutant, B. melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not essential for the intracellular survival of B. melitensis in human MDM.
authors.affiliation: U. R. Biologie Moléculaire, Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre Dame de la Paix, Namur, Belgium. Philippe.Denoel@fundp.ac.be
authors.forename: P A
authors.initials: PA
authors.lastname: Denoel
authors.forename: R M
authors.initials: RM
authors.lastname: Crawford
authors.forename: M S
authors.initials: MS
authors.lastname: Zygmunt
authors.forename: A
authors.initials: A
authors.lastname: Tibor
authors.forename: V E
authors.initials: VE
authors.lastname: Weynants
authors.forename: F
authors.initials: F
authors.lastname: Godfroid
authors.forename: D L
authors.initials: DL
authors.lastname: Hoover
authors.forename: J J
authors.initials: JJ
authors.lastname: Letesson
mesh_terms: D001426:Bacterial Proteins; D017347:Brucella melitensis / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D003573:Cytochrome b Group / Q000235:genetics*; D005293:Ferritins / Q000235:genetics*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology; D009154:Mutation; D018384:Oxidative Stress; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D005293:Ferritins; C018032:bacterioferritin