In vitro plasmid-encoded resistance to quinolones.
The possibility of plasmid-encoded quinolone resistance is explored in two model systems. In the first, increasing amounts of wild-type gyrA allele moderately increased minimum inhibitory concentrations to quinolone antibiotics. In the second model, a mutant gyrA allele encoded by a multicopy plasmid produced a quinolone resistance phenotype upon its expression in a quinolone-susceptible Escherichia coli strain.
Gómez-Gómez
J M
JM
Servicio de Microbiología, Hospital Ramón y Cajal, Madrid, Spain.
Blázquez
J
J
Espinosa De Los Monteros
L E
LE
Baquero
M R
MR
Baquero
F
F
Martínez
J L
JL
D000483:Alleles; D000890:Anti-Infective Agents / Q000494:pharmacology*; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D024841:Fluoroquinolones; D018628:Gene Dosage; D005816:Genetic Complementation Test; D010957:Plasmids
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II