A human 28S ribosomal RNA retropseudogene.
A human genomic clone designated LhrRAX3 isolated from an X chromosome-specific library was found to have a 28S ribosomal RNA retropseudogene symbolized as RNRP2 within a 12.5-kb human DNA insert. The sequence of the rRNA retropseudogene has an identity of 96% with about 300 nucleotides at the 3'-terminus of the human 28S rRNA gene. RNRP2 is flanked by a pair of perfect direct repeats of 16 nucleotides, the hallmark characteristic of a processed pseudogene having been integrated into the genome. The structural element has a long A-rich tract at its 3'-end, apparently the result of an aberrant polyadenylation event of a RNA polymerase I transcript, prior to its subsequent reverse transcription and retroposition into the genome. An Alu repeat sequence truncated by 80 nucleotides at the 5'-region occurs about 800 base pairs downstream and is of opposite orientation to RNRP2. The Alu element is bounded by 16-nucleotide direct repeats and is a member of the Alu Y subfamily.
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Department of Biological Sciences, University of North Texas, Denton 76203, USA.
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D001483:Base Sequence; D002874:Chromosome Mapping; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D011544:Pseudogenes; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
D012339:RNA, Ribosomal, 28S; D018626:Retroelements