Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras.
RasGAPs supply a catalytic residue, termed the arginine finger,into the active site of Ras thereby stabilizing the transition state of the GTPase reaction and increasing the reaction rate by more than one thousand-fold, in good agreement with the structure of the Ras.RasGAP complex.
Ahmadian
M R
MR
Stege
P
P
Scheffzek
K
K
Wittinghofer
A
A
D001120:Arginine / Q000737:chemistry*; D001665:Binding Sites; D020558:GTP Phosphohydrolases / Q000737:chemistry*; D020690:GTPase-Activating Proteins; D006160:Guanosine Triphosphate / Q000378:metabolism; D011506:Proteins / Q000737:chemistry*; D020703:ras GTPase-Activating Proteins; D018631:ras Proteins / Q000737:chemistry*
D020690:GTPase-Activating Proteins; D011506:Proteins; D020703:ras GTPase-Activating Proteins; D006160:Guanosine Triphosphate; D001120:Arginine; D020558:GTP Phosphohydrolases; D018631:ras Proteins