Three-dimensional cultures of keratinocytes and an application to in vitro-amyloid production of cutaneous amyloidosis.
Some three-dimensional culture models of the skin were reviewed including our systems using a collagen dermal substitute and a matrix dermal substitute. No obvious junctional structures, such as hemidesmosomes and the lamina densa, were formed between the basal keratinocytes and the dermal substitutes, when the cytoplasmic membrane of the basal keratinocytes directly faced the collagenous materials. On the other hand, when the cytoplasmic membrane of the basal keratinocytes faced the preformed basement membrane, the type IV collagen film, or the extracellular matrix gel, an organized interaction occurred between the basal keratinocytes and the dermal substitute through hemidesmosomes and a rudimentary lamina densa. Keratinocyte differentiation in the suprabasal layers seemed to be closely related to such a basal cell differentiation. Our preliminary examination of the experimental amyloid production by the epidermal sheet from the lesional skin of patients with primary localized cutaneous amyloidosis suggested that the basal cells in the transplanted lesional epidermis produced amyloid fibrils in our in vitro culture model. This is another use of the three-dimensional culture models of the skin in addition to the application of the systems to wound treatment.
Horiguchi
Y
Y
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
Suzuki
S
S
Toda
K
K
Ueda
M
M
Leigh
I M
IM
Imamura
S
S
D000682:Amyloid / Q000378:metabolism*; D000686:Amyloidosis / Q000378:metabolism*; D001485:Basement Membrane / Q000276:immunology / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000276:immunology / Q000378:metabolism; D005109:Extracellular Matrix / Q000276:immunology / Q000378:metabolism; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008954:Models, Biological; D012867:Skin / Q000648:ultrastructure; D012871:Skin Diseases / Q000378:metabolism*; D017768:Skin, Artificial
D000682:Amyloid; D003094:Collagen