title: Detrimental effect of hydrosalpinx fluid on the development and blastulation of mouse embryos in vitro.
abstract: OBJECTIVE
  To study the effects of human hydrosalpinx fluid on mouse embryo blastulation rate.
  
  
  METHODS
  Comparison of mouse embryo blastulation rate in media containing increasing concentrations of hydrosalpinx fluid.
  
  
  METHODS
  Tertiary care center.
  
  
  METHODS
  Women undergoing laparoscopic evaluation or treatment for infertility noted to have hydrosalpinx or paratubal cyst.
  
  
  METHODS
  Exposure of mouse embryos to hydrosalpinx or paratubal cyst fluid collected during laparoscopy.
  
  
  METHODS
  Blastulation rate of mouse embryos.
  
  
  RESULTS
  Culture of mouse embryos at 0% (controls), 0.3%, 0.6%, and 0.9% hydrosalpinx fluid concentrations demonstrated significantly lower blastulation rate at each level compared with the controls.
  
  
  CONCLUSIONS
  Hydrosalpinx fluid is highly embryotoxic.
authors.affiliation: Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey (UMDNJ)-Robert Wood Johnson Medical School, New Brunswick 08901, USA.
authors.forename: R
authors.initials: R
authors.lastname: Sachdev
authors.forename: E
authors.initials: E
authors.lastname: Kemmann
authors.forename: M K
authors.initials: MK
authors.lastname: Bohrer
authors.forename: I
authors.initials: I
authors.lastname: el-Danasouri
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D005184:Fallopian Tube Diseases / Q000503:physiopathology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D011247:Pregnancy