Cold-sensitive rad52 alleles of yeast.
We have characterized two rad52 mutations that are cold-sensitive for growth on MMS agar. The mutations change residues 61 and 69, respectively, in the 504 amino-acid open reading frame. Neither mutation has a profound effect on mitotic crossing-over or on gene conversion. One has a severe deficiency in sporulation at all temperatures, while the other has a partial deficiency and reduced spore viability. Both mutants are retarded in growth on MMS agar by a high-copy plasmid expressing RAD51.
Nguyen
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MM
Department of Biochemistry, University of Minnesota, 435 Delaware St. SE, Minneapolis, MN 55455-0347, USA.
Livingston
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DM
D000362:Agar; D002455:Cell Division / Q000235:genetics; D003080:Cold Temperature; D003434:Crossing Over, Genetic; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D016153:Genes, Suppressor; D006023:Glycoproteins / Q000494:pharmacology; D008540:Meiosis; D008741:Methyl Methanesulfonate; D008938:Mitosis; D009154:Mutation; D051135:Rad51 Recombinase; D051721:Rad52 DNA Repair and Recombination Protein; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D013172:Spores, Fungal / Q000235:genetics
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D006023:Glycoproteins; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D029701:Saccharomyces cerevisiae Proteins; D000362:Agar; D008741:Methyl Methanesulfonate; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific