Interferences of o-raffinose cross-linked hemoglobin in three methods for serum creatinine.
The interferences of o-raffinose cross-linked hemoglobin (Hemolink) were examined and compared in two serum creatinine methods on the Hitachi 717 [Boehringer Mannheim (BMC) and Synermed] and in an enzymatic creatinine method on the Vitros 750 (Johnson & Johnson). Interference was considered significant when the change in creatinine concentration from the control exceeded the 95% confidence limits of each method. Significant interference was observed for the BMC/Hitachi 717 method with Hemolink > or = 5 g/L. No interference was observed for the Synermed/Hitachi 717 assay with Hemolink up to 30 g/L in normal samples and up to 50 g/L in samples with increased creatinine. No interference was observed for the Vitros 750 assay with Hemolink up to 50 g/L at both normal and increased concentrations of creatinine. Although the BMC/Hitachi 717 method was considered unacceptable, the Synermed/Hitachi 717 and the Vitros 750 methods allow accurate quantification of serum creatinine in the presence of Hemolink.
Ali
A C
AC
Hemosol Inc., Etobicoke, Ontario, Canada.
Mihas
C C
CC
Campbell
J A
JA
D016477:Artifacts; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D016001:Confidence Intervals; D003404:Creatinine / Q000097:blood*; D006454:Hemoglobins; D006801:Humans; D011887:Raffinose / Q000031:analogs & derivatives*; D012016:Reference Values; D015203:Reproducibility of Results
D006454:Hemoglobins; C097124:O-raffinose cross-linked human hemoglobin; D003404:Creatinine; D011887:Raffinose