Large-scale immunohistological staining using polyethylene glycol-embedded sections mounted on 96-well plates for monoclonal antibody screening.
We have developed a rapid, large-scale immunohistological staining method for monoclonal antibody screening. Sections embedded in polyethylene glycol are mounted on 96-well plates and then subjected to immunological procedures using a 96-well format. With this method, we can prepare 10 plates (960 samples) in 1 day and then perform immunohistological procedures in 1 day by using an enzyme-linked immunosorbent assay-like 96-well format. Thus, we can avoid difficulties seen in conventional immunohistological methods that use sections mounted on glass slides. This method offers high-throughput screening of monoclonal antibodies. In addition, it has the potential of being automated.
Komiya
T
T
Hirohashi Cell Configuration Project, ERATO, Japan Science and Technology Corporation (JST), 5-9-4 Tokodai, Tsukuba, 300-26, Japan.
Tanigawa
Y
Y
Oda
S
S
Hirohashi
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002645:Chickens; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D009132:Muscles / Q000737:chemistry; D011092:Polyethylene Glycols; D012867:Skin / Q000737:chemistry; D013737:Testis / Q000737:chemistry; D014981:Xenopus
D000911:Antibodies, Monoclonal; D011092:Polyethylene Glycols