title: Detection of rotavirus in sewage and creek water: efficiency of the concentration method.
abstract: Simian rotavirus SA-11 experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method, a mean recovery of 81% +/- 7.5 of the SA-11 virus, was achieved.
authors.affiliation: Departamento de Microbiologia, Instituto de Cièncias, Biomédicas, Universidade de São Paulo, Brasil.
authors.forename: D U
authors.initials: DU
authors.lastname: Mehnert
authors.forename: K E
authors.initials: KE
authors.lastname: Stewien
authors.forename: C M
authors.initials: CM
authors.lastname: Hársi
authors.forename: A P
authors.initials: AP
authors.lastname: Queiroz
authors.forename: J M
authors.initials: JM
authors.lastname: Candeias
authors.forename: J A
authors.initials: JA
authors.lastname: Candeias
mesh_terms: D018929:Cell Culture Techniques; D005618:Fresh Water; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000517:prevention & control*; D012722:Sewage / Q000821:virology*; D014773:Virology / Q000379:methods; D014871:Water Microbiology
chemical_list: D012722:Sewage