Evidence for hybridization by multilocus enzyme electrophoresis and random amplified polymorphic DNA between Leishmania braziliensis and Leishmania panamensis/guyanensis in Ecuador.
The taxonomic attribution of four Leishmania stocks isolated from humans in Ecuador has been explored by both multilocus enzyme electrophoresis and random amplified polymorphic DNA. For three loci, MLEE results showed patterns suggesting a heterozygous state for a diploid organism, while the corresponding homozygous states are characteristic of the Leishmania panamensis/guyanensis complex and Leishmania braziliensis, respectively. Other enzyme loci showed characters attributable to either the L. panamensis/ guyanensis complex or L. braziliensis. RAPD profiles exhibited for several primers a combination of the Leishmania panamensis/ guyanensis complex and L. braziliensis characters. These data hence suggest that the four stocks are the result of hybridization between L. panamensis/guyanensis and L. braziliensis. MLEE data show that the results cannot be attributed to either mixture of stocks, or an F1 in the framework of a simple Mendelian inheritance.
Bañuls
A L
AL
Centre d'Etudes sur le Polymorphisme des Microorganismes (CEPM), Centre National de la Recherche Scientifique (CNRS)/Institut Français de Recherche Scientifique pour le Développement en Coopération (ORSTOM), Montpellier, France. banuls@cepm.mpl.ozstom.
Guerrini
F
F
Le Pont
F
F
Barrera
C
C
Espinel
I
I
Guderian
R
R
Echeverria
R
R
Tibayrenc
M
M
D000818:Animals; D002678:Chimera / Q000235:genetics*; D016054:DNA, Protozoan / Q000032:analysis; D004484:Ecuador; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007892:Leishmania braziliensis / Q000201:enzymology / Q000235:genetics*; D018334:Leishmania guyanensis / Q000201:enzymology / Q000235:genetics*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology; D007897:Leishmaniasis, Mucocutaneous / Q000469:parasitology; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique
D016054:DNA, Protozoan; D007527:Isoenzymes