Induction of calcyclin after ischemic injury to rat kidney.
Genes differentially expressed after acute renal ischemic injury were identified using differential display-polymerase chain reaction (DD-PCR). Messenger RNA for calcyclin, a member of the S100 family of calcium-binding proteins, is increased in kidneys by 6 h following ischemic injury to rats compared with sham surgery. The level of calcyclin mRNA is increased 10-fold by 1 day postinjury and declines thereafter. In situ hybridization demonstrates little calcyclin mRNA in kidneys of sham-operated rats. However, calcyclin protein is present in glomeruli and distal tubules (DT). Compared with kidneys from sham-operated controls, both calcyclin mRNA and protein expression are increased at 1-3 days following ischemic injury in the thick ascending limb of Henle, the DT, and in damaged regenerating segments of proximal tubules. By 7 days postischemia there is a reduction in mRNA and protein expression. Calcyclin could play a role in the regulation of renal cell proliferation and regeneration in the recovery process after acute ischemic injury.
Lewington
A J
AJ
George M. O'Brien Kidney and Urological Diseases Center, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Padanilam
B J
BJ
Hammerman
M R
MR
D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D004815:Epidermal Growth Factor / Q000096:biosynthesis; D017403:In Situ Hybridization; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; D014158:Transcription, Genetic
D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489132:S100a6 protein, rat; D004815:Epidermal Growth Factor