Human vestibular schwannoma growth in the nude mouse: evaluation of a modified subcutaneous implantation model.
OBJECTIVE
Based on the hypothesis that vestibular schwannomas can be successfully implanted and grown in the nude mouse model, an in vivo experiment was designed for subcutaneous implantation of solid vestibular schwannoma tissue.


BACKGROUND
Vestibular schwannomas are benign tumors arising from Schwann cells of cranial nerve VIII. Little in vivo research has been carried out with these tumors, due in part to the difficulty to grow cells in culture or maintain tumor in an animal model. Recently, vestibular schwannomas have been implanted in nude mice with moderate success. The current study evaluates a modification of prior techniques in an effort to establish a dependable research model.


METHODS
Thirty-six nude mice were implanted with variable-sized vestibular schwannoma tissue from three human subjects. Volumes implanted ranged from 14-170 mm3. Mice were observed for 28 days and individual volumes recalculated. Eleven of the mice were observed for a total of 56 days with volumes re-evaluated, and tumors subsequently were removed for assessment of viability and vascularity.


RESULTS
At 28 days, 36 tumors (100%) showed take with 34 tumors (94%) showing macroscopic growth. The 11 tumors observed for 56 days showed a trend of stable or decreased size at 56 days compared with that of the 28-day measurement. Overall growth from time of implantation to measurements at 56 days was noted in 8 (73%) of 11 tumors when measured at the skin and in 10 (91%) of 11 tumors when direct tumor volume was measured. One hundred percent of tumors evaluated microscopically at 56 days was viable. All tumors at the time of removal had significant vascularity with a mean of 70.68% (SD = 23.42) of surface covered with vessels. There were no significant differences in take and growth for the larger tumor specimens compared with those of smaller sizes.


CONCLUSIONS
Human vestibular schwannomas successfully can be implanted and maintained in the subcutaneous pocket of the nude mouse. This in vivo tumor model provides a reliable, accessible base for further research with vestibular schwannomas.
Stidham
K R
KR
California Ear Institute at Stanford, Palo Alto 94304, USA.
Roberson
J B
JB
D000818:Animals; D046508:Culture Techniques; D004428:Ear Neoplasms / Q000473:pathology* / Q000601:surgery*; D005260:Female; D006085:Graft Survival; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009442:Neurilemmoma / Q000473:pathology* / Q000601:surgery*; D009464:Neuroma, Acoustic / Q000473:pathology / Q000601:surgery; D010865:Pilot Projects; D014722:Vestibule, Labyrinth / Q000473:pathology* / Q000601:surgery*