IL-1 modifies system A transport activity in human rheumatoid synovial cells.
Given the importance of interleukin-1 in both rheumatic diseases and the modulation of cell metabolic activities, we studied the action of this cytokine on the neutral amino acid transport A system on rheumatoid synovial cells. In these cells IL-1 (1 ng/ml) induced amino transport stimulation from 5 min to 5 h. This effect was obtained only after a starvation period. No concentration-related effect was found for IL-1-stimulated MeAIB uptake, and the IL-1-mediated MeAIB uptake stimulation is independent of protein synthesis. Neosynthesis or post-translational maturation of protein transport is a prerequisite for obtaining this effect. In conclusion, rheumatoid synovial cells exhibit a higher sensitivity for IL-1 than osteoarthritic ones, probably related to their intense metabolic activity.
Le Maire
V
V
Laboratoire de Biochimie Cellulaire de l'Inflammation, Université Paris Sud, France.
Hernvann
A
A
Le Marechal
H
H
Ekindjian
O G
OG
Aussel
C
C
D000596:Amino Acids / Q000502:physiology; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D001692:Biological Transport; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000378:metabolism
D000596:Amino Acids; D007375:Interleukin-1; D011500:Protein Synthesis Inhibitors; D015091:beta-Alanine; C012924:2,2-dimethyl-beta-alanine; D003513:Cycloheximide