title: Cytochrome b6/f complex from the cyanobacterium Synechocystis 6803: evidence of dimeric organization and identification of chlorophyll-binding subunit.
abstract: Fractionation of photosynthetic membranes from the cyanobacterium Synechocystis 6803 by polyacrylamide gel electrophoresis in the presence of Deriphat-160 allowed the isolation of a number of pigmented bands. Two of them, with molecular masses of 240+/-20 and 110+/-15 kDa respectively, showed peroxidase activity and, by means of polypeptide composition, immunoblotting and N-terminal sequencing, were identified as dimeric and monomeric cytochrome b6/f complexes, containing 1.3+/-0.35 chlorophyll molecules per cytochrome f. Further fractionation of monomeric complexes by mild gel electrophoresis in the presence of sodium dodecyl sulfate indicated that it is the cytochrome b6 polypeptide which provides the actual binding site for the chlorophyll molecule observed in the complex.
authors.affiliation: Dipartimento di Biologia, Università di Padova, Padua, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Poggese
authors.forename: P
authors.initials: P
authors.lastname: Polverino de Laureto
authors.forename: G M
authors.initials: GM
authors.lastname: Giacometti
authors.forename: F
authors.initials: F
authors.lastname: Rigoni
authors.forename: R
authors.initials: R
authors.lastname: Barbato
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry; D002734:Chlorophyll / Q000378:metabolism*; D000458:Cyanobacteria / Q000737:chemistry*; D003573:Cytochrome b Group / Q000737:chemistry* / Q000378:metabolism*; D045346:Cytochrome b6f Complex; D019281:Dimerization; D014450:Electron Transport Complex III; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006418:Heme / Q000737:chemistry; D007096:Imidoesters / Q000737:chemistry; D015151:Immunoblotting; D007202:Indicators and Reagents / Q000737:chemistry; D007506:Iron-Sulfur Proteins / Q000032:analysis / Q000276:immunology; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D007096:Imidoesters; D007202:Indicators and Reagents; D007506:Iron-Sulfur Proteins; C037989:Rieske iron-sulfur protein; D002734:Chlorophyll; C034266:N-lauryl iminodipropionate; D006418:Heme; D045346:Cytochrome b6f Complex; D014450:Electron Transport Complex III