title: A useful method for the synthesis of 8 azido-guanosine tri- and tetraphosphate: important substrates for many enzymatic reactions.
abstract: 8-N3 GMP was synthesised from 8-BrGMP by the addition of LiN3. 8-N3GMP was then phosphorylated to N3-GDP and N3-GTP by controlled enzymatic reaction. 8-N3GDP can be converted to N3-ppGpp with crude Rel A, which phosphorylates the 3'-OH of GDP.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad, India.
authors.forename: N
authors.initials: N
authors.lastname: Nagesh
authors.forename: D
authors.initials: D
authors.lastname: Chatterji
mesh_terms: D002855:Chromatography, Thin Layer; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000302:isolation & purification
chemical_list: D006160:Guanosine Triphosphate