Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: linking 130 protein spots with their respective genes.
Following the complete sequencing of the genome of the univellular cyanobacterium, Synechocystis sp. strain PCC6803 within our institute, a protein-gene linkage map of this photosynthetic microorganism was successfully constructed for 130 high abundance proteins present on two-dimensional gels. An additional six proteins were analyzed, but were probably encoded extrachromosomally. In order to demonstrate the usefulness of this protein-gene linkage map, we analyzed the changes that occur in cellular proteins after illumination of PCC6803 cells. The results indicate that this protein-gene linkage map greatly simplifies the identification process of such modulated genes. After illumination, at least three distinctive spots with reduced intensity were detected on two-dimensional gels and the corresponding genes of two of these were successfully identified as chaperonin 2 and a Tortula ruralis rehydrin-related gene. Thus, the combination of the protein-gene linkage map and two-dimensional gel electrophoresis should permit a comprehensive analyses of the proteins encoded by the genome (i.e., "proteome") of this photosynthetic autotroph. This post-genome project represents a productive way of exploiting the information obtained from the sequencing of the cyanobacterium genome.
Sazuka
T
T
Laboratory of DNA Technology, Kazusa DNA Research Institute, Chiba, Japan.
Ohara
O
O
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification*; D002874:Chromosome Mapping; D000458:Cyanobacteria / Q000737:chemistry / Q000235:genetics* / Q000528:radiation effects; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005798:Genes, Bacterial; D008040:Genetic Linkage; D016680:Genome, Bacterial; D008027:Light; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010449:Peptide Mapping / Q000379:methods
D001426:Bacterial Proteins