Reactivity of the new monoclonal antibody '22' with meningococcal strains isolated from patients and carriers in Greece.
Previous studies found that the majority of Neisseria meningitidis isolates from either patients or carriers in Greece do not react with the monoclonal antibodies used at present in the whole-cell ELISA (WCE) for determination of serotype and subtype antigens. A new monoclonal antibody designated '22' produced by the National Meningococcal Reference Laboratory in the Czech Republic was assessed in the whole-cell ELISA with 257 non-typable meningococcal strains from both patients (52) and carriers (205). The carrier strains included 34 non-typable isolates from two immigrant populations: ethnic Greeks who have immigrated from Russia since 1989 (19/75) and Kurdish refugees (15/34). Approximately 10% of the meningococcal strains isolated from patients and 11.7% of the carrier strains reacted with the reagent. Although the majority of meningococcal isolates from resident Greeks were not typable with the antibody, 11/19 (57.9%) of the carrier strains from Russian immigrants and 4/15 (20%) of those from the Kurdish refugees reacted with the new reagent.
Tzanakaki
G
G
National Meningococcal Reference Laboratory, National School of Public Health, Athens, Greece.
Kriz
P
P
Kremastinou
J
J
Musilek
M
M
Smart
L E
LE
Blackwell
C C
CC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D002353:Carrier State; D002648:Child; D002675:Child, Preschool; D004641:Emigration and Immigration; D004797:Enzyme-Linked Immunosorbent Assay; D006115:Greece / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008589:Meningococcal Infections / Q000453:epidemiology* / Q000276:immunology*; D008875:Middle Aged; D009345:Neisseria meningitidis / Q000145:classification* / Q000276:immunology; D012606:Scotland / Q000453:epidemiology; D012703:Serotyping
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial