A function for CD2 on murine B cells?
CD2 is expressed on murine B cells, probably as a result of a chromosomal translocation event during speciation. There are no activating antibodies to mouse CD2, but when activated non-specifically, only T cells up-regulate their expression of CD2. We investigated the expression and function of CD2 on B cells using mice transgenic for human CD2 under the control of a modified version of the autologous promoter/enhancer that included the immunoglobulin enhancer. This construction directs expression of human CD2 to the B cell compartment as well as to the T cell compartment. In this paper, we confirm that activating pairs of anti-CD2 monoclonal antibodies are mitogenic for mouse T cells transgenic for human CD2. In contrast, mouse B cells that express similar amounts of human CD2 are not stimulated to proliferate by equivalent doses of these antibodies. We were also unable to show any functional consequence for these B cells as a result of CD2 ligation.
Keogh
M C
MC
Department of Immunology, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, UK.
Elliot
J
J
Norton
T
T
Lake
R A
RA
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000378:metabolism / Q000502:physiology*; D018801:CD2 Antigens / Q000235:genetics* / Q000276:immunology / Q000502:physiology*; D019013:CD40 Antigens / Q000276:immunology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II / Q000235:genetics; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000096:biosynthesis; D015759:Ionomycin / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D013601:T-Lymphocytes / Q000378:metabolism; D013872:Thionucleosides / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D018801:CD2 Antigens; D019013:CD40 Antigens; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D013872:Thionucleosides; D006151:Guanosine; D015759:Ionomycin; C037031:8-thioguanosine