Genetic immunization with adeno-associated virus vectors expressing herpes simplex virus type 2 glycoproteins B and D.
Intramuscular injection of mice with an adeno-associated virus (AAV) vector expressing herpes simplex virus type 2 glycoprotein B led to the generation of both gB-specific major histocompatibility complex class I-restricted cytotoxic T lymphocytes and anti-gB antibody. AAV-mediated immunization was more potent than plasmid DNA or protein in generating antibody responses.
Manning
W C
WC
Chiron Corporation, Emeryville, California 94608, USA. william_manning@cc.chiron.com
Paliard
X
X
Zhou
S
S
Pat Bland
M
M
Lee
A Y
AY
Hong
K
K
Walker
C M
CM
Escobedo
J A
JA
Dwarki
V
V
D000818:Animals; D000917:Antibody Formation; D003602:Cytotoxicity, Immunologic; D000229:Dependovirus; D005822:Genetic Vectors; D018258:Herpesvirus 2, Human / Q000276:immunology*; D007273:Injections, Intramuscular; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013997:Time Factors; D019444:Vaccines, DNA / Q000008:administration & dosage; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage
D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C044525:glycoprotein B, herpes simplex virus type 2; C040570:glycoprotein D-herpes simplex virus type 2