title: Morphometric analysis of growth retardation in fetal lambs following experimental infection of pregnant ewes with border disease virus.
abstract: The onset of growth retardation was investigated in fetal lambs following experimental infection of pregnant ewes with Border Disease virus (BDV) on day 53 of pregnancy. Fetuses from control and infected ewes were harvested at weekly intervals between day 60 and day 95 of gestation and morphometric studies were completed on tibial radiographs and tibial growth cartilage metaphyseal junctions. Mean tibial areas were significantly reduced (P < 0.01) in fetuses from infected ewes at 35 and 42 days after infection and growth cartilage metaphyseal junctions were less mature in fetuses from infected ewes at 42 days after infection. Positive immunostaining for BDV antigen was demonstrated in the brains of all fetuses from infected ewes between 14 and 42 days after infection. Attempts to demonstrate BDV antigen in bone proved unsuccessful. It is concluded that intrauterine growth retardation is an early manifestation of BDV infection in lambs and that the process is initiated shortly following infection of the fetus.
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, University College Dublin, Ireland.
authors.forename: J F
authors.initials: JF
authors.lastname: Caffrey
authors.forename: A M
authors.initials: AM
authors.lastname: Dudgeon
authors.forename: W J
authors.initials: WJ
authors.lastname: Donnelly
authors.forename: B J
authors.initials: BJ
authors.lastname: Sheahan
authors.forename: G J
authors.initials: GJ
authors.lastname: Atkins
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D001882:Border Disease / Q000276:immunology / Q000503:physiopathology* / Q000821:virology; D018083:Border disease virus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D005317:Fetal Growth Retardation / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D005865:Gestational Age; D006132:Growth Plate / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D007150:Immunohistochemistry; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D011859:Radiography; D012756:Sheep; D012757:Sheep Diseases / Q000503:physiopathology* / Q000821:virology; D013977:Tibia / Q000000981:diagnostic imaging / Q000196:embryology / Q000254:growth & development
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral