Integrin-dependent tyrosine phoshorylation and cytoskeletal translocation of Tec in thrombin-activated platelets.
Using a specific polyclonal anti-Tec antibody, we have shown that Tec is expressed in human platelets. In addition, Tec was found to undergo tyrosine phosphorylation during platelet activation. The phosphorylation increased after 1 min and remained stable after 3 min of thrombin treatment. The tetrapeptide RGDS inhibited more than 90% of thrombin-induced tyrosine phosphorylation of Tec and blocked its translocation to the cytoskeleton. These results suggest that Tec participates in platelet signaling downstream of integrin activation.
Laffargue
M
M
INSERM U326, IFR30, Hôpital Purpan, Toulouse, France.
Monnereau
L
L
Tuech
J
J
Ragab
A
A
Ragab-Thomas
J
J
Payrastre
B
B
Raynal
P
P
Chap
H
H
D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000201:enzymology* / Q000378:metabolism; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D016023:Integrins / Q000502:physiology*; D007700:Kinetics; D007947:Leukemia, Megakaryoblastic, Acute; D010766:Phosphorylation; D015539:Platelet Activation; D011505:Protein-Tyrosine Kinases / Q000097:blood* / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D013917:Thrombin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism*
D003598:Cytoskeletal Proteins; D016023:Integrins; D014443:Tyrosine; C089848:Tec protein-tyrosine kinase; D011505:Protein-Tyrosine Kinases; D013917:Thrombin