Detection of basal levels and induction of delta opioid receptor mRNA in murine splenocytes.
Activation of delta opioid receptors (DOR) modulates calcium mobilization, interleukin-2 production, chemotaxis and proliferation of T-lymphocytes. Recent reports indicate that lymphocytes and mononuclear cells may express mRNA transcripts for DOR. The investigations reported herein show that low levels of DOR were consistently detected by RT-PCR amplification of RNA from freshly obtained Balb/c murine splenocytes, both weanling and adult. Culturing cells without stimulation increased DOR levels and concanavalin A apparently reduced this; DOR was preferentially expressed in a T-cell-enriched fraction. Thus, the expression of DOR mRNA by unactivated splenocytes is modulated by culture and con A in the T-cell fraction.
Sharp
B M
BM
Endocrine-Neuroscience and Neuroimmunomodulation Res, Labs., Hennepin County Medical Center and University of Minnesota, Minneapolis 55404, USA. sharp002@maroon.tc.umn.edu
Shahabi
N
N
McKean
D
D
Li
M D
MD
McAllen
K
K
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D017465:Receptors, Opioid, delta / Q000235:genetics*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D014158:Transcription, Genetic
D012333:RNA, Messenger; D017465:Receptors, Opioid, delta