Expression of progesterone receptor mRNA in the first trimester human placenta.
The human placenta produces large quantities of progesterone, the function and target tissues of action of which during pregnancy are not completely understood. Although it has been suggested that placenta itself could be a potential target tissue for progesterone action, there is no conclusive evidence for the presence of progesterone receptors in the human placenta. We provide evidence for the expression of progesterone receptor mRNA in the human placenta by reverse transcription coupled to polymerase chain reaction (RT-PCR). This was further confirmed by Southern hybridization, restriction analysis and sequencing of the PCR amplified fragment.
Shanker
Y G
YG
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Sharma
S C
SC
Rao
A J
AJ
D001483:Base Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012333:RNA, Messenger / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000235:genetics*; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D014599:Uterus / Q000378:metabolism
D017931:DNA Primers; D012333:RNA, Messenger; D011980:Receptors, Progesterone