title: Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus.
abstract: A 10 mM concentration of lithium does not interfere with reverse transcription (RT) or PCR. Sampling of cervicovaginal fluid by vaginal washing, with lithium (10 mM) in the washing buffer as a marker of dilution, may be utilized to accurately determine in HIV-infected women, by quantitative RT-PCR, the genital shedding of acellular HIV RNA at the level of the mucosa itself.
authors.affiliation: Laboratoire de Virologie, Hôpital Broussais, Paris, France.
authors.forename: A S
authors.initials: AS
authors.lastname: Mohamed
authors.forename: P
authors.initials: P
authors.lastname: Becquart
authors.forename: H
authors.initials: H
authors.lastname: Hocini
authors.forename: P
authors.initials: P
authors.lastname: Métais
authors.forename: M
authors.initials: M
authors.lastname: Kazatchkine
authors.forename: L
authors.initials: L
authors.lastname: Bélec
mesh_terms: D002582:Cervix Mucus / Q000821:virology*; D005260:Female; D006678:HIV / Q000302:isolation & purification* / Q000502:physiology; D015658:HIV Infections / Q000503:physiopathology* / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D007507:Therapeutic Irrigation; D017201:Virus Shedding / Q000235:genetics
chemical_list: D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase