title: External biodegradable supporting conduit protects endothelium in vein graft in arterial interposition.
abstract: The prevention of circumferential distension could reduce structural damage in arteriovenous grafts. We studied the effect of an external biodegradable supporting conduit on the endothelium and extracellular matrix in vein graft in a pig model. Cephalic vein control grafts (Group I) and jugular veins wrapped in a vicryl mesh tube (I.D. 4 mm) (Group II) were implanted into autologous carotid arteries (n = 14). The grafts were explanted after 1 and 24 hours and at 1 and 3 weeks and evaluated by ELISA for endothelial DNA synthesis and by immunohistoenzymic assays for cells and extracellular matrix. In group I an initial loss of endothelial and smooth muscle cells along with elastin breakdown was followed by an impaired endothelial regeneration and significant graft wall thickening. The elastic tissue was replaced by collagen type I and chondroitin sulfate accumulations, which included a disarray of alpha-smooth muscle actin positive cells. The endothelium was preserved in group II. After 3 weeks the circumferential elastin layers were densified, distended and separated from the endothelium by a neointimal growth of irregular thickness. Biodegradable perivenous conduit minimized endothelial injury and allowed the partial preservation of elastin fibers and smooth muscle cells in the arteriovenous graft. It did not however, prevent myofibroblastic cell proliferation and triggered a macrophagic reaction.
authors.affiliation: Centre de Recherches Chirurgicales Henri Mondor, CNRS URA D 1431, Association Claude Bernard, Créteil, France.
authors.forename: L S
authors.initials: LS
authors.lastname: Bambang
authors.forename: M
authors.initials: M
authors.lastname: Moczar
authors.forename: L
authors.initials: L
authors.lastname: Lecerf
authors.forename: D
authors.initials: D
authors.lastname: Loisance
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004730:Endothelium, Vascular / Q000033:anatomy & histology* / Q000166:cytology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D013552:Swine; D014182:Transplantation, Autologous; D014680:Veins / Q000637:transplantation*
chemical_list: D000199:Actins; D001672:Biocompatible Materials; D002809:Chondroitin Sulfates; D003094:Collagen; D004247:DNA