Enhanced expression of endothelial oxidized low-density lipoprotein receptor (LOX-1) in hypertensive rats.
Oxidized low-density lipoprotein (Ox-LDL) has been implicated in the attenuated endothelium-dependent vasodilatation in atherosclerotic arteries and, possibly, in hypertension. The aim of the present study was to investigate gene expression of recently-identified endothelial Ox-LDL receptor (LOX-1) in hypertensive state. SHR-SP, WKY, Dahl salt-sensitive (DS) and salt-resistant rats (DR) were fed salt-loaded or control diet. RNA was extracted from the aorta and vein. LOX-1 expression was examined by Northern blotting. LOX-1 mRNA was low in the aorta and vein of WKY, whereas it was markedly upregulated in those of SHR-SP. LOX-1 expression was low in the aorta of DR on both diets and of DS on a control diet, whereas it was elevated in that of salt-loaded DS. These results indicated that LOX-1 expression in the aorta and vein was upregulated in hypertensive rats, which may be involved in the impaired endothelium-dependent vasodilation in these rats.
Nagase
M
M
Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan.
Hirose
S
S
Sawamura
T
T
Masaki
T
T
Fujita
T
T
D000818:Animals; D001011:Aorta; D001794:Blood Pressure; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000378:metabolism*; D006973:Hypertension / Q000235:genetics / Q000503:physiopathology; D008297:Male; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY; D011973:Receptors, LDL / Q000096:biosynthesis*; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E; D012982:Sodium, Dietary; D013045:Species Specificity; D014158:Transcription, Genetic; D014680:Veins
D017931:DNA Primers; C495136:OLR1 protein, rat; D012333:RNA, Messenger; D011973:Receptors, LDL; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E; D012982:Sodium, Dietary