Rescue of a viral gene from VERO cells latently infected with influenza virus B/Lee/40.
By growing VERO cells infected with 5 PFU/cell of influenza virus B/Lee/40, a latently infected culture was readily established (L/V cells). The cells continued to multiply stably, excreting a small amount of virus in the beginning, which sharply declined according to cell division to undetectable level by day 9. However, nucleotide sequences for all the 8 genes of B/Lee/40 as well as their mRNAs were amplified from L/V cells on day 50 or later by RT-PCR. Moreover, from the 95-day-old L/V cells, a persisting NP gene of B/Lee/40 was rescued into infectious virus particles upon superinfection with homotypic influenza virus B/Yamagata/1/73.
Tobita
K
K
Department of Virology, Jichi Medical School, Minami-Kawachi-Machi, Tochigi-Ken, 329-04, Japan. tobikiyo@jichiac.jp
Tanaka
T
T
Hayase
Y
Y
D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D002522:Chlorocebus aethiops; D017931:DNA Primers; D004285:Dogs; D005814:Genes, Viral; D009981:Influenza B virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D007700:Kinetics; D008969:Molecular Sequence Data; D019251:Nucleocapsid / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D016415:Sequence Alignment; D013997:Time Factors; D014709:Vero Cells; D014779:Virus Replication
D017931:DNA Primers; D012333:RNA, Messenger; D012367:RNA, Viral