title: The effect of chronic alcohol use on the heart before and after transplantation in an experimental model in the rat.
abstract: BACKGROUND
  Alcohol has potential deleterious effects on donor heart function. This study was conducted in rats to determine whether long-term alcohol ingestion produces impaired hemodynamic performance while maintaining a normal left ventricular ejection fraction in donor hearts before transplantation and whether donor cardiac function is affected after heart transplantation.
  
  
  METHODS
  Rats fed 30% alcohol in their drinking water for 12 weeks were compared with rats fed a normal diet. Left ventricular ejection fraction was measured by echocardiography with Simpson and single plane Dodge formulas in living sedated rats after 10 and 12 weeks of alcohol feeding. Explanted heart function was assessed before and 3 days after heterotopic heart transplantation (no immunosuppression) with a Langendorff preparation.
  
  
  RESULTS
  Blood ethanol levels at 4 and 8 weeks were 0.08 +/- 0.04 and 0.08 +/- 0.09 gm/dl. Left ventricular ejection fraction was similar in the group fed an alcohol diet for 12 weeks when compared with the control group (65.4% +/- 1.6% vs. 66.5% +/- 2.9%, p = 0.33). Explanted alcohol-fed hearts before transplantation had significantly lower maximum and developed pressures and had a blunted response to 0.1 ml 10(-9) mol/L isoproterenol. After transplantation alcohol-fed hearts had significantly lower maximum and developed pressures and decreased maximum rates of pressure rise and pressure decline. Allografts (ACI to Lewis) exhibited decreased function in comparison with isografts (ACI to ACI).
  
  
  CONCLUSIONS
  Alcohol feeding for 12 weeks in rats does not affect pretransplantation left ventricular ejection fraction, but it impairs explanted heart function, both before and after transplantation, resulting in a subclinical cardiomyopathy that is worsened by the presence of allograft rejection. Long-term alcohol exposure and rejection have independent, additive detrimental effects on left ventricular performance of the transplanted heart. Alcohol-exposed hearts may not be suitable donors.
authors.affiliation: Division of Cardiothoracic Surgery, Cedars-Sinai Medical Center, Los Angeles, Calif. 90048, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Takkenberg
authors.forename: H M
authors.initials: HM
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Trento
authors.forename: A
authors.initials: A
authors.lastname: Popov
authors.forename: D
authors.initials: D
authors.lastname: Freimark
authors.forename: K
authors.initials: K
authors.lastname: Eghbali
authors.forename: C H
authors.initials: CH
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Blanche
authors.forename: L S
authors.initials: LS
authors.lastname: Czer
mesh_terms: D000818:Animals; D002310:Cardiomyopathy, Alcoholic / Q000503:physiopathology / Q000601:surgery*; D004452:Echocardiography; D000431:Ethanol / Q000493:pharmacokinetics; D016027:Heart Transplantation / Q000502:physiology*; D006439:Hemodynamics / Q000502:physiology*; D008297:Male; D051381:Rats; D011912:Rats, Inbred ACI; D013318:Stroke Volume / Q000502:physiology; D014019:Tissue Donors; D016042:Transplantation, Heterotopic / Q000502:physiology; D016277:Ventricular Function, Left / Q000502:physiology
chemical_list: D000431:Ethanol