title: In vitro plasmid-encoded resistance to quinolones.
abstract: The possibility of plasmid-encoded quinolone resistance is explored in two model systems. In the first, increasing amounts of wild-type gyrA allele moderately increased minimum inhibitory concentrations to quinolone antibiotics. In the second model, a mutant gyrA allele encoded by a multicopy plasmid produced a quinolone resistance phenotype upon its expression in a quinolone-susceptible Escherichia coli strain.
authors.affiliation: Servicio de Microbiología, Hospital Ramón y Cajal, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Gómez-Gómez
authors.forename: J
authors.initials: J
authors.lastname: Blázquez
authors.forename: L E
authors.initials: LE
authors.lastname: Espinosa De Los Monteros
authors.forename: M R
authors.initials: MR
authors.lastname: Baquero
authors.forename: F
authors.initials: F
authors.lastname: Baquero
authors.forename: J L
authors.initials: JL
authors.lastname: Martínez
mesh_terms: D000483:Alleles; D000890:Anti-Infective Agents / Q000494:pharmacology*; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D024841:Fluoroquinolones; D018628:Gene Dosage; D005816:Genetic Complementation Test; D010957:Plasmids
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II