title: Towards the development of a bioartificial pancreas: a 13C NMR study on the effects of alginate/poly-L-lysine/alginate entrapment on glucose metabolism by beta TC3 mouse insulinoma cells.
abstract: We have utilized 13C NMR spectroscopy to investigate glucose metabolism in the mouse insulinoma Beta TC3 cell line. Cells were cultured and examined both as monolayers and entrapped in alginate/poly-L-lysine/alginate beads. Entrapped cultures were tested at 3 and 30 days post-entrapment. The purpose of this study was to assess whether the entrapped environment affects glucose metabolism and insulin secretion. Both monolayer and entrapped cultures were fed with 10 mM [1-(13)C]-glucose for 4 hrs. prior to extraction with perchloric acid. Our data revealed that beta TC3 cells possess a reduced tricarboxylic acid (TCA) activity in the entrapment cultures, and that they metabolized pyruvate primarily via pyruvate dehydrogenase regardless of the mode or age of the culture.
authors.affiliation: Frederik Philips Magnetic Resonance Research Center, Department of Radiology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: I
authors.initials: I
authors.lastname: Constantinidis
authors.forename: N E
authors.initials: NE
authors.lastname: Mukundan
authors.forename: M P
authors.initials: MP
authors.lastname: Gamcsik
authors.forename: A
authors.initials: A
authors.lastname: Sambanis
mesh_terms: D000464:Alginates / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002247:Carbon Isotopes; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D005947:Glucose / Q000378:metabolism*; D020723:Glucuronic Acid; D006019:Glycolysis; D006603:Hexuronic Acids; D007340:Insulinoma / Q000188:drug therapy / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D019397:Pancreas, Artificial; D010472:Perchlorates / Q000737:chemistry; D011107:Polylysine / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000464:Alginates; D002247:Carbon Isotopes; D006603:Hexuronic Acids; D010472:Perchlorates; D011107:Polylysine; D019344:Lactic Acid; D020723:Glucuronic Acid; D005947:Glucose; D004801:Eosine Yellowish-(YS)