Detection of rotavirus in sewage and creek water: efficiency of the concentration method.
Simian rotavirus SA-11 experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method, a mean recovery of 81% +/- 7.5 of the SA-11 virus, was achieved.
Mehnert
D U
DU
Departamento de Microbiologia, Instituto de Cièncias, Biomédicas, Universidade de São Paulo, Brasil.
Stewien
K E
KE
Hársi
C M
CM
Queiroz
A P
AP
Candeias
J M
JM
Candeias
J A
JA
D018929:Cell Culture Techniques; D005618:Fresh Water; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000517:prevention & control*; D012722:Sewage / Q000821:virology*; D014773:Virology / Q000379:methods; D014871:Water Microbiology
D012722:Sewage