Carbohydrate protection of enzyme structure and function against guanidinium chloride treatment depends on the nature of carbohydrate and enzyme.
Baker's yeast cells accumulate osmolytes as a response to several stress conditions such as high-temperature and low-temperature shifts, dehydration, or osmotic stress. One of the major osmolytes that accumulates is trehalose, which plays an essential role affecting the survival of yeast at the time of stress. In this report, we show that trehalose efficiently protects the function and the structure of two yeast cytosolic enzymes against chemical denaturation by guanidinium chloride. Other sugars tested also protected yeast pyrophosphatase and glucose-6-phosphate dehydrogenase structure against guanidinium chloride effects, but were not as efficient at protecting enzyme activity. The thermostable pyrophosphatase from Bacillus stearothermophilus was also protected by several sugars against the chaotropic properties of guanidinium chloride, but was only protected by trehalose against functional inactivation. The function of the membrane-embedded H+-ATPase from yeast could not be protected by any of the tested sugars, although all of the sugars protected its structure from guanidinium-chloride-induced unfolding. The results presented in this study suggest that several sugars are able to prevent protein unfolding induced by a chaotropic compound. However, prevention of functional inactivation depends on the nature of the sugar. Trehalose was the most efficient, being able to protect many cytosolic enzymes against guanidinium chloride. The efficiency of protection also depended on the nature of the protein tested. This might explain why trehalose is one of the osmolytes accumulated in yeast and also why it is not the only osmolyte to accumulate.
Sola-Penna
M
M
Departamento de Fármacos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Brasil. maurosp@pharma.ufrj.br
Ferreira-Pereira
A
A
Lemos
A P
AP
Meyer-Fernandes
J R
JR
D002241:Carbohydrates / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D004798:Enzymes / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D043564:Inorganic Pyrophosphatase; D007700:Kinetics; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D017434:Protein Structure, Tertiary / Q000187:drug effects; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D011755:Pyrophosphatases / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014199:Trehalose / Q000494:pharmacology
D002241:Carbohydrates; D004791:Enzyme Inhibitors; D004798:Enzymes; D006146:Guanidines; D014199:Trehalose; D005954:Glucosephosphate Dehydrogenase; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase; D006180:Proton-Translocating ATPases; D019791:Guanidine