Pentoxifylline alters class-specific immunoglobulin synthesis in resuscitated burn injury.
Pentoxifylline (PTXF) is a hemorheologic agent that can attenuate microvascular alterations induced by injury, resulting in improved microcirculatory flow. Burn injury-induced immunologic suppression may be caused by alterations in microvascular flow. We studied the effects of PTXF on humoral immunity in resuscitated burn injury. Male AKR mice (n = 80) were randomized to four groups: SHAM, BURN, PTXF-BURN, and NS-BURN (saline burn). Animals were resuscitated with Ringer's lactate and morphine sulfate and put to death at days 1 and 4 after injury. PTXF-BURN animals received PTXF (50 mg/kg intraperitoneally) at the time of resuscitation. Splenic lymphocytes (1 x 10(6) cells/well) were cultured with lipopolysaccharide (LPS) (2.5 and 10 micrograms/ml)). LPS-stimulated in vitro class-specific immunoglobulin (Ig) production was determined by enzyme-linked immunosorbent assay from splenic cell culture supernatants. Burn injury induced a reduction in immunoglobulin (Ig) M synthesis on postburn day 4. PTXF treatment was associated with an increase in IgG production, but a greater depression in IgM synthesis than burn injury alone. PTXF administration during resuscitation in burn injury does not ameliorate the burn-induced depression in IgM synthesis and results in significant potential adverse modulation of humoral immune function.
Napolitano
L M
LM
Department of Surgery, Baltimore Veterans Administration Medical Center, MD 21201, USA.
Koruda
M J
MJ
Baker
C C
CC
Meyer
A A
AA
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002056:Burns / Q000150:complications / Q000276:immunology*; D005440:Fluid Therapy; D007075:Immunoglobulin M / Q000096:biosynthesis* / Q000187:drug effects; D066298:In Vitro Techniques; D007263:Infusions, Parenteral; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D010431:Pentoxifylline / Q000276:immunology / Q000494:pharmacology*; D011897:Random Allocation; D013154:Spleen / Q000166:cytology; D014665:Vasodilator Agents / Q000276:immunology / Q000494:pharmacology*
D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D014665:Vasodilator Agents; D010431:Pentoxifylline