title: The effect of early weaning on dentin formation and dentinal caries in rats.
abstract: The effect of early weaning on caries progression, dentin formation, and dentin mineralization was examined in four groups of rats. Two groups received a normal diet and were weaned on day 18 or 21, and another two received a sucrose-rich diet and were weaned on day 18 or 21. At age 35 days the lower molars were sectioned sagittally, and the areas of dentin formation and of the dentinal caries were quantified. The width of the predentin zone was measured from histologically stained sections of maxillary molars. Early weaning reduced dentin formation in the group on the high-sucrose diet only the first days; later this effect was partially caught up with. A high-sucrose diet significantly increased caries frequency and extension of caries lesions compared with a normal diet in both early weaned and normally weaned groups. The effect of early weaning on caries frequency and extension in the high-sucrose group was insignificant compared with the normally weaned group on a high-sucrose diet. The predentin zone was wider in the sucrose groups than in the control groups at the end of the experiment. These results indicate that the effect of sucrose on dentin formation was dependent on the stage of physiologic dentin formation, but early weaning as such did not affect this.
authors.affiliation: Institute of Dentistry, University of Oulu, Finland.
authors.forename: E L
authors.initials: EL
authors.lastname: Hietala
authors.forename: J
authors.initials: J
authors.lastname: Autio
authors.forename: M
authors.initials: M
authors.lastname: Larmas
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents; D004396:Coloring Agents; D003731:Dental Caries / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D003743:Dental Enamel / Q000473:pathology; D003804:Dentin / Q000473:pathology*; D003810:Dentinogenesis / Q000502:physiology*; D004032:Diet; D004037:Diet, Cariogenic; D019422:Dietary Sucrose / Q000008:administration & dosage / Q000009:adverse effects; D018450:Disease Progression; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008297:Male; D008963:Molar; D010118:Oxytetracycline; D051381:Rats; D017207:Rats, Sprague-Dawley; D014074:Tooth Calcification / Q000502:physiology; D014886:Weaning
chemical_list: D000900:Anti-Bacterial Agents; D004396:Coloring Agents; D019422:Dietary Sucrose; D005452:Fluoresceins; D005456:Fluorescent Dyes; C007740:fluorexon; D010118:Oxytetracycline