Identity of bovine growth hormone and peptidylglycine monooxygenase.
The C-terminal alpha-amidation of peptides is one of the most important events in prohormone and neuropeptide processing. Peptide amidation is a two-step process catalyzed by peptidylglycine (hydroxylating) monooxygenase (B. A. Eipper et al., 1983, Proc. Natl. Acad. Sci. USA 80, 5144-5148) followed by dismutation of the resultant hydroxylated peptide to peptide amide and glyoxylate, stimulated by alpha-hydroxyglycine amidating dealkylase (K. Takahashi et al., 1990, Arch. Biochem. Biophys. 169, 524-530). Previous reports on peptidylglycine monooxygenase from bovine pituitary have generated substantial disagreement as to its molecular size. We have reinvestigated the purification of this enzyme and we find that peptidylglycine monooxygenase activity from fresh bovine pituitary is entirely due to a previously unrecognized catalytic function of growth hormone (somatotropin).
Downey
E
E
Department of Biochemistry, University College Galway, Ireland.
Donlon
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D013006:Growth Hormone / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes; D010902:Pituitary Gland / Q000201:enzymology*; D011499:Protein Processing, Post-Translational; D017421:Sequence Analysis
D009097:Multienzyme Complexes; D013006:Growth Hormone; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase