RU 41740 (Biostim) stimulates the production of granulocyte macrophage colony-stimulating factor and interleukin-8 by human bronchial epithelial cells in vitro.
1. In this study, we observed the effects of RU 41740 (Biostim) on the production of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-8 (IL-8) in human bronchial epithelial cells in vitro. 2. Cytokine production was assessed by enzyme-linked immunosorbent assay. 3. We report that epithelial cells spontaneously released both cytokines and that RU 41740 induced a significant increase in production of IL-8 and GM-CSF. 4. This is the first observation of a stimulatory effect of an immunostimulating compound used in humans on cytokine production by epithelial cells.
Aloui
R
R
Service de Pneumologie, Centre Hospitalier Lyon-Sud, Pierre Bénite, France.
Gormand
F
F
Guibert
B
B
Corallo
F
F
Perrin Fayolle
M
M
Pacheco
Y
Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000368:Aged; D001426:Bacterial Proteins / Q000494:pharmacology*; D001980:Bronchi / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D016209:Interleukin-8; C037567:glycoprotein, Klebsiella pneumoniae; D016178:Granulocyte-Macrophage Colony-Stimulating Factor