title: The phagocytosis activity during conventional and laparoscopic operations in the rat. A preliminary study.
abstract: BACKGROUND
  Numerous experimental and clinical investigations indicate that the mononuclear phagocyte system (MPS) has a relevant function in terms of physiological defense against tumor metastasis and bacterial infection. Consequently, a point of major interest is the influence of surgical techniques on the MPS function.
  
  
  METHODS
  The model investigation examines the phagocytosis activity of the rat's MPS during conventional fundoplication (group 1, n = 10), laparoscopic fundoplication using a pneumoperitoneum (group 2, n = 10), and gasless laparoscopic fundoplication (group 3, n = 10). The MPS function is evaluated by an intravascular carbon clearance test (G. Biozzi).
  
  
  RESULTS
  The fastest carbon elimination half-life was found in group 3. By way of contrast, there was a significant increase of carbon half-life in group 2 (p < 0.005). Even group 1 caused less MPS depression (p < 0.1) than group 2.
  
  
  CONCLUSIONS
  Gasless laparoscopic procedures have a favorable effect on phagocytosis activity. The CO2 pneumoperitoneum seems to be the main reason for a decreased antigen elimination in laparoscopic treatments.
authors.affiliation: Department of General Surgery, Johann-Wolfgang-Goethe-University, Theodor-Stern-Kai 7, D-60590 Frankfurt/Main, Germany.
authors.forename: C N
authors.initials: CN
authors.lastname: Gutt
authors.forename: P
authors.initials: P
authors.lastname: Heinz
authors.forename: W
authors.initials: W
authors.lastname: Kaps
authors.forename: V
authors.initials: V
authors.lastname: Paolucci
mesh_terms: D000818:Animals; D002244:Carbon / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D018662:Fundoplication; D006207:Half-Life; D006551:Hernia, Hiatal / Q000601:surgery*; D010535:Laparoscopy / Q000379:methods*; D016014:Linear Models; D008297:Male; D010587:Phagocytosis / Q000502:physiology*; D010865:Pilot Projects; D011028:Pneumoperitoneum, Artificial / Q000379:methods; D051381:Rats; D012680:Sensitivity and Specificity; D013514:Surgical Procedures, Operative / Q000379:methods*
chemical_list: D002244:Carbon