Diagnosis of bladder cancer by analysis of the allelic loss of the p53 gene in urine samples using blunt-end single-strand conformation polymorphism.
The novel approach of blunt-end single-strand conformation polymorphism (SSCP) has been applied in the analysis of urine samples from bladder-cancer patients for detecting loss of heterozygosity (LOH) of 3 polymorphic markers in the p53 gene. Of the 28 urine samples examined by SSCP analysis of blunt-ended DNA fragments using a fluorescence-based automated sequencer, 16 were informative in more than 1 of the 3 polymorphic markers at the p53 locus and 8 (50.0%) showed allelic loss of the p53 gene. In analysis of resected tumor tissues, LOH of the p53 gene was detected in 8 of 8 informative samples (100%) with T1 and higher stages and/or Grade 2 and Grade 3 tumors, while it was detected in 6 (75.0%) urine samples obtained from these 8 patients. This new diagnostic modality enables sensitive detection of tumor cells in urine samples and would be applicable for diagnostic bladder cancer with invasive character.
Sugano
K
K
Division of Clinical Laboratory, National Cancer Center Hospital, Tokyo, Japan. ksugano@gan2.ncc.go.jp
Tsutsumi
M
M
Nakashima
Y
Y
Yamaguchi
K
K
Ohkura
H
H
Kakizoe
T
T
Sekiya
T
T
D000483:Alleles; D002872:Chromosome Deletion; D004247:DNA / Q000652:urine*; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000032:analysis; D005091:Exons; D016158:Genes, p53; D006580:Genetic Carrier Screening; D006801:Humans; D007438:Introns; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000652:urine*
D017931:DNA Primers; D004273:DNA, Neoplasm; D004247:DNA