Chemotaxis of Amoeba proteus in the developing pH gradient within a pocket-like chamber studied with the computer assisted method.
A new "U" shaped, pocket-like chamber was used to observe the chemotactic responses of individual cells. This method permits monitoring of both the development of the concentration gradient of a tested substance and cell locomotion. We investigated the chemotactic responses of Amoeba proteus and observed that the amoebae moved in positively and negatively developing [H+] gradients towards the solution of lower pH in a pH range 5.75-7.75. The chemotactic response of amoebae to [H+] gradients required the presence of extracellular calcium ions. It was blocked and random locomotion was restored by the replacement of calcium with magnesium in the cell medium. Time-lapse video recording and data processing were accomplished with computer-assisted methods. This made it possible to compare selected methods of data presentation and analysis for cells locomoting in isotropic and anisotropic conditions. The cell trajectories were determined and displayed in circular diagrams, lengths of cell tracks and final cell displacements were estimated and a few parameters characterizing cell locomotion were computed.
Korohoda
W
W
Department of Cell Biology, The J. Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Golda
J
J
Sroka
J
J
Wojnarowicz
A
A
Jochym
P
P
Madeja
Z
Z
D000656:Amoeba / Q000166:cytology*; D000818:Animals; D002633:Chemotaxis / Q000502:physiology*; D003584:Cytological Techniques / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008124:Locomotion / Q000502:physiology; D018715:Microscopy, Video / Q000379:methods