[Cell proliferation in migrating colonies of epidermoid carcinoma A-431 cells in culture].
Migrating colonies of asymmetrical shape are formed in the culture of human epidermoid carcinoma A-431 at a low density of plating. Antiproliferative factors arrest migration, thus suggesting a certain correlation between cell migration and proliferation in this culture. However, under these conditions, a certain level of proliferation can be retained upon complete suppression of migration. Radioautographic studies did not reveal unequal distribution of the labelled cells were also in migrating colonies. Upon suppression and subsequent stimulation of migration, the labelled cells were also distributed equally in most colonies. Thus, the capacity of colonies for migration does not depend on cell proliferation in a certain part of the colony, but is determined by the proliferative potential of the entire colony. The influence of cell position in the colony on the cell cycle length remains unclear. Actively migrating colonies are capable of DNA synthesis, thus suggesting that proliferation and migration are fully compatible in individual cells.
Samarova
A V
AV
Voroteliak
E A
EA
Vasil'ev
A V
AV
Terskikh
V V
VV
D001345:Autoradiography; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D002465:Cell Movement; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D013997:Time Factors; D014407:Tumor Cells, Cultured
D003470:Culture Media; D016895:Culture Media, Serum-Free; D004273:DNA, Neoplasm