A new membrane filtration medium for simultaneous detection and enumeration of Escherichia coli and total coliforms.
Recovery of total coliforms and Escherichia coli on a new membrane filtration (MF) medium was evaluated with 25 water samples from seven states. Testing of the new medium, m-ColiBlue24 broth, was conducted according to a U.S. Environmental Protection Agency protocol. For comparison, this same protocol was used to measure recovery of total coliforms and E. coli with two standard MF media, m-Endo broth and mTEC broth. E. coli recovery on the new medium was also compared to recovery on nutrient agar supplemented with 4-methylumbelliferyl-beta-D-glucuronide. Comparison of specificity, sensitivity, false positive error, undetected target error, and overall agreement indicated E. coli recovery on m-ColiBlue24 was superior to recovery on mTEC for all five parameters. Recovery of total coliforms on the new medium was comparable to recovery on m-Endo.
Grant
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MA
Hach Company, Ames, Iowa 50010, USA.
D015169:Colony Count, Microbial / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D003470:Culture Media; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D004926:Escherichia coli / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005374:Filtration / Q000379:methods; D012680:Sensitivity and Specificity; D014871:Water Microbiology
D003470:Culture Media