Immunomagnetic separation and PCR for detection of Mycobacterium ulcerans.
We have developed a technique based on the use of monodisperse magnetic beads to isolate Mycobacterium ulcerans from heterogenous mixtures, prior to PCR amplification. Using this method, we were able to detect M. ulcerans in water samples taken from Phillip Island, Australia, the site of several outbreaks of M. ulcerans disease in recent times.
Roberts
B
B
Department of Microbiology and Immunology, James Cook University of North Queensland, Townsville, Australia. Brenda.Roberts@jcu.edu.au
Hirst
R
R
D000907:Antibodies, Bacterial; D001315:Australia / Q000453:epidemiology; D004196:Disease Outbreaks; D005618:Fresh Water; D018189:Immunomagnetic Separation / Q000379:methods*; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology; D019911:Mycobacterium ulcerans / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014871:Water Microbiology
D000907:Antibodies, Bacterial