title: PCB/toxaphene group separation on silica prior to congener specific determination of toxaphene residues in fish and other samples by GC/ECD.
abstract: A precise quantification of toxaphene residues of environmental samples by gas chromatography/ electron capture detection (GC/ECD) requires the separation of the bulk of the polychlorinated biphenyls (PCBs) from the compounds of technical toxaphene (CTT) fraction. For this reason, a PCB/CTT group separation on silica was developed. B8-1413 (Parlar #26) and B7-515 (Parlar #32) eluted as first and last out of ten important CTT standards, and can be used to determine the elution volume of the CTT fraction on silica. GC/ECD quantification of CTTs was possible after separation of PCBs on 8.0 g activated silica eluted with 48 mL n-hexane followed by quantitative elution of CTTs with n-hexane/toluene (65:35; v/v). This method is a compromise between separation efficiency and consumption of material. Finally, eight CTTs were quantified in cod liver samples from Iceland and the Baltic Sea.
authors.affiliation: Institut für Ernährung und Umwelt, Friedrich-Schiller-Universität Jena, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Krock
authors.forename: W
authors.initials: W
authors.lastname: Vetter
authors.forename: B
authors.initials: B
authors.lastname: Luckas
mesh_terms: D000818:Animals; D004785:Environmental Pollutants / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D005399:Fishes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007306:Insecticides / Q000032:analysis* / Q000302:isolation & purification*; D008099:Liver / Q000737:chemistry; D008297:Male; D011078:Polychlorinated Biphenyls / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D014112:Toxaphene / Q000032:analysis* / Q000302:isolation & purification*
chemical_list: D004785:Environmental Pollutants; D007306:Insecticides; D012822:Silicon Dioxide; D014112:Toxaphene; D011078:Polychlorinated Biphenyls