[DAN gene].
DAN gene has been isolated by means of differential screening method between rat fibroblast 3Y1 and Rous sarcoma virus-transformed 3Y1 (SR-Y1) cells. DAN expression was suppressed in some of the transformed cells and its re-expression reverted the various transformed phenotypes such as colony formation in soft agar and tumor formation in mice. When DAN was overexpressed in 3Y1 cells, these cell showed the retardation of the entry into the S phase. DAN cDNA encodes a 27-kD protein which consists of 178 amino acids. The deduced amino acid sequence has no homology with known proteins. The amino terminal of DAN is highly hydrophobic and DAN is indeed secreted into the culture medium. This secreted DAN, when added to the culture of SR-3Y1 cells, suppressed DNA synthesis. Human DAN is mapped to 1p36.11-p36.13, a region known to have highly significant linkage with the genesis and/or progression of human neuroblastoma. Aberrant bands on Southern blot were detected in some of the neuroblastoma cases. Rat and human genomic DANs were isolated. CAT and the electrophoretic mobility shift assays revealed the presence of possible positive and negative regulatory elements at -57 approximately +118 and -1,232 approximately -987 of rat genome, respectively.
Sakiyama
S
S
Chiba Cancer Center Research Institute.
Ozaki
T
T
D000818:Animals; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D016147:Genes, Tumor Suppressor; D006801:Humans; D009419:Nerve Tissue Proteins; D009447:Neuroblastoma / Q000235:genetics; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D018797:Cell Cycle Proteins; C490087:NBL1 protein, human; C085763:Nbl1 protein, rat; D009419:Nerve Tissue Proteins; D011506:Proteins