Bacteroides J-37, a human intestinal bacterium, produces alpha-glucuronidase.
beta-Glucuronidases of mammalian tissues metabolized glycyrrhizin (18 beta-glycyrrhetinic acid, beta-D-glucuronyl alpha-D-glucuronic acid, GL) to glycyrrhetinic acid (GA) via 18 beta-glycyrrhetinic acid alpha-D-glucuronic acid (GAMG); they hydrolyzed beta-glucuronic acid conjugates better than alpha-glucuronic acid conjugates. However, human intestinal bacteria directly metabolized GL to GA, and minorly to GA via GAMG. Bacteroides J-37, isolated from human intestinal bacteria, transformed GL or GAMG to GA, but not baicalin; it produced alpha-glucuroniase, which hydrolyzed the alpha-linkage of glucuronic acid conjugates. alpha-Glucuronidase of Bacteroides J-37 hydrolyzed alpha-glucuronic acid conjugates better than beta-glucuronic acid conjugates. beta-Glucuronidase from E. coli, a human intestinal bacterium, hydrolyzed baicalin to baicalein, but did not transform GL.
Kim
D H
DH
College of Pharmacy, Kyung-Hee University, Seoul, Korea.
Jang
I S
IS
Lee
S W
SW
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001439:Bacteroides / Q000201:enzymology*; D044950:Flavanones; D005419:Flavonoids / Q000378:metabolism; D005965:Glucuronates / Q000737:chemistry; D020723:Glucuronic Acid; D006026:Glycoside Hydrolases / Q000378:metabolism*; D066298:In Vitro Techniques; D007422:Intestines / Q000382:microbiology*; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats
D001426:Bacterial Proteins; D044950:Flavanones; D005419:Flavonoids; D005965:Glucuronates; C006680:baicalein; D020723:Glucuronic Acid; D006026:Glycoside Hydrolases; C096734:alpha-glucuronidase