title: Outer membrane-associated serine protease of intestinal spirochetes.
abstract: Pathogenic intestinal spirochetes cause damage to the intestinal mucosa of humans and animals by an unknown mechanism. The purpose of this study was to assess the pathogenic intestinal spirochetes Serpulina hyodysenteriae, Serpulina pilosicoli, and Brachyspira aalborgi and the non-pathogenic commensal intestinal spirochetes Serpulina innocens and Treponema succinifaciens for protease activity. A partially heat stable, subtilisin-like, serine protease was identified in the outer membrane of all spirochetes and thus may be essential for survival in the intestinal environment. The outer membrane protease may indirectly contribute to intestinal damage caused by pathogenic spirochetes during association with the mucosal surface of the host.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln 68583-0905, USA.
authors.forename: N
authors.initials: N
authors.lastname: Muniappa
authors.forename: G E
authors.initials: GE
authors.lastname: Duhamel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D001717:Birds; D017401:Brachyspira hyodysenteriae / Q000737:chemistry / Q000201:enzymology*; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D013381:Subtilisins / Q000032:analysis* / Q000378:metabolism; D013552:Swine
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D010455:Peptides; D013381:Subtilisins