title: Evaluation of bedside prothrombin time and activated partial thromboplastin time measurement by coagulation analyzer CoaguCheck Plus in various clinical settings.
abstract: In the present study CoaguCheck Plus (CCP), a coagulation test system using whole blood, was evaluated with respect to its comparability with widely distributed conventional routine coagulation assays. A correlation of r = 0.997 (p < 0.0001) was found between INR of CCP-prothrombin time (CCP-PT) and Thrombotest (KC-1 analyzer) in patients on oral anticoagulant therapy. A correlation of r = 0.899 (p < 0.001) between CCP-aPTT and Actin ES aPTT (STA analyzer) was found in heparinized patients. Impaired hepatic hepatic coagulation factor synthesis in liver cirrhosis patients was detected by CCP-PT with a sensitivity of 0.75 and by Normotest (STA analyzer) with a sensitivity of 0.92. Those patients with normal CCP-PT values and liver disease had, only mild reductions (> 30% of normals) in coagulation factors II, V, VII or X. CCP-aPTT was also performed in patients with a deficiency in the so called endogenous coagulation factors VIII, IX, XI and XII. CCP-aPTT showed a sensitivity similar to that of Actin FS aPTT in the detection even of mild deficiencies in factors VIII, IX and XII; factor XI deficiency was however detected only in patients with severe (< 12% of normals) disease; lupus anticoagulants were detected with a high sensitivity.
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, University of Vienna, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Ruzicka
authors.forename: S
authors.initials: S
authors.lastname: Kapiotis
authors.forename: P
authors.initials: P
authors.lastname: Quehenberger
authors.forename: S
authors.initials: S
authors.lastname: Handler
authors.forename: S
authors.initials: S
authors.lastname: Hornykewycz
authors.forename: A
authors.initials: A
authors.lastname: Michitsch
authors.forename: K
authors.initials: K
authors.lastname: Huber
authors.forename: D
authors.initials: D
authors.lastname: Clemens
authors.forename: M
authors.initials: M
authors.lastname: Susan
authors.forename: I
authors.initials: I
authors.lastname: Pabinger
authors.forename: S
authors.initials: S
authors.lastname: Eichinger
authors.forename: B
authors.initials: B
authors.lastname: Jilma
authors.forename: W
authors.initials: W
authors.lastname: Speiser
mesh_terms: D001780:Blood Coagulation Tests / Q000295:instrumentation*; D006493:Heparin / Q000627:therapeutic use; D006801:Humans; D007202:Indicators and Reagents; D008111:Liver Function Tests; D016682:Lupus Coagulation Inhibitor / Q000032:analysis; D010314:Partial Thromboplastin Time; D019095:Point-of-Care Systems; D011517:Prothrombin Time; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D007202:Indicators and Reagents; D016682:Lupus Coagulation Inhibitor; D006493:Heparin