title: Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion.
abstract: TRPC3 (or Htrp3) is a human member of the trp family of Ca2+-permeable cation channels. Since expression of TRPC3 cDNA results in markedly enhanced Ca2+ influx in response to stimulation of membrane receptors linked to phospholipase C (Zhu, X., J. Meisheng, M. Peyton, G. Bouley, R. Hurst, E. Stefani, and L. Birnbaumer. 1996. Cell. 85:661-671), we tested whether TRPC3 might represent a Ca2+ entry pathway activated as a consequence of depletion of intracellular calcium stores. CHO cells expressing TRPC3 after intranuclear injection of cDNA coding for TRPC3 were identified by fluorescence from green fluorescent protein. Expression of TRPC3 produced cation currents with little selectivity for Ca2+ over Na+. These currents were constitutively active, not enhanced by depletion of calcium stores with inositol-1,4,5-trisphosphate or thapsigargin, and attenuated by strong intracellular Ca2+ buffering. Ionomycin led to profound increases of currents, but this effect was strictly dependent on the presence of extracellular Ca2+. Likewise, infusion of Ca2+ into cell through the patch pipette increased TRPC3 currents. Therefore, TRPC3 is stimulated by a Ca2+-dependent mechanism. Studies on TRPC3 in inside-out patches showed cation-selective channels with 60-pS conductance and short (<2 ms) mean open times. Application of ionomycin to cells increased channel activity in cell-attached patches. Increasing the Ca2+ concentration on the cytosolic side of inside-out patches (from 0 to 1 and 30 microM), however, failed to stimulate channel activity, even in the presence of calmodulin (0.2 microM). We conclude that TRPC3 codes for a Ca2+-permeable channel that supports Ca2+-induced Ca2+-entry but should not be considered store operated.
authors.affiliation: Institut für Pharmakologie, Thielallee 69-73, Freie Universität Berlin, D-14195 Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Zitt
authors.forename: A G
authors.initials: AG
authors.lastname: Obukhov
authors.forename: C
authors.initials: C
authors.lastname: Strübing
authors.forename: A
authors.initials: A
authors.lastname: Zobel
authors.forename: F
authors.initials: F
authors.lastname: Kalkbrenner
authors.forename: A
authors.initials: A
authors.lastname: Lückhoff
authors.forename: G
authors.initials: G
authors.lastname: Schultz
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D016466:CHO Cells / Q000737:chemistry / Q000502:physiology; D002118:Calcium / Q000378:metabolism* / Q000493:pharmacokinetics*; D015220:Calcium Channels / Q000502:physiology; D002412:Cations / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D015870:Gene Expression / Q000502:physiology; D015640:Ion Channel Gating / Q000502:physiology; D007473:Ion Channels / Q000235:genetics*; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D050052:TRPC Cation Channels; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D002412:Cations; D007473:Ion Channels; D007476:Ionophores; D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; D014665:Vasodilator Agents; D000804:Angiotensin II; D015759:Ionomycin; D002118:Calcium