Association of DNA with nuclear matrix in in vitro assembled nuclei induced by rDNA from Tetrahymena shanghaiensis in Xenopus egg extracts.
Nuclei assembled in vitro from Xenopus egg extracts and DNA display many properties known from in vivo nuclei. However, the distribution pattern of DNA in such nuclei remains unknown. We introduced rDNA from Tetrahymena macronuclei into Xenopus egg extracts and examined the association of specific DNA sequences with the nuclear matrix (NM) of the nuclei formed. We found that the 5' non-transcribed spacers (5'-NTS) specifically bound to the NM as previously shown in normal Tetrahymena macronuclei.
Chen
Y
Y
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, People's Republic of China.
Zhang
B
B
Li
X
X
Zhai
Z
Z
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002474:Cell-Free System; D004275:DNA, Ribosomal / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D018014:Gene Transfer Techniques; D015530:Nuclear Matrix / Q000502:physiology* / Q000648:ultrastructure; D009865:Oocytes / Q000502:physiology; D013768:Tetrahymena / Q000235:genetics*; D014981:Xenopus
D004275:DNA, Ribosomal