title: Rabbit muscle GAPDH: non-phosphorylating dehydrogenase activity induced by hydrogen peroxide.
abstract: Incubation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) with micromolar hydrogen peroxide concentrations does not alter the catalytic properties of GAPDH in the reaction of oxidative phosphorylation of glyceraldehyde-3-phosphate, but endows the enzyme with the ability to catalyze the reaction in the absence of inorganic phosphate, producing NADH and 3-phosphoglycerate. The reaction is supposed to occur as a result of intramolecular acyl transfer from Cys-149 to a sulfenic acid form of Cys-153, followed by hydrolysis of the intermediate. The 'mildly oxidized' form of the enzyme can be easily converted back to the form unable to catalyze glyceraldehyde-3-phosphate oxidation in the absence of phosphate, by the addition of thiols.
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia. muric@bac.genebee.msu.su
authors.forename: E V
authors.initials: EV
authors.lastname: Schmalhausen
authors.forename: V I
authors.initials: VI
authors.lastname: Muronetz
authors.forename: N K
authors.initials: NK
authors.lastname: Nagradova
mesh_terms: D000215:Acylation; D000818:Animals; D018053:Arsenites / Q000494:pharmacology; D001665:Binding Sites; D002384:Catalysis; D003545:Cysteine; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006868:Hydrolysis; D007700:Kinetics; D018482:Muscle, Skeletal / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010766:Phosphorylation; D011817:Rabbits
chemical_list: D018053:Arsenites; D005994:Glycerophosphates; D009243:NAD; C029620:alpha-glycerophosphoric acid; D006861:Hydrogen Peroxide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D003545:Cysteine; C015001:arsenite