title: [Neospora caninum causes abortions in a cattle herd in North Rhine Westphalia].
abstract: In a cattle herd in North Rhine-Westphalia a series of eight abortions occurred between 15th August and 14th October, 1996. Four of the aborted fetuses were histopathologically, virologically, bacteriologically and parasitologically examined. A multifocal necrotising encephalitis was observed in two fetuses. In addition, the liver of two fetuses and the placenta of one fetus contained infected foci. In three fetuses protozoan stages were detected which were identified as N. caninum by immunohistochemistry. A polymerase chain reaction conducted with histological material revealed N. caninum-DNA in the placenta of an aborted fetus. When seven cows that had aborted were tested for antibodies directed against N. caninum, six animals were positive by immunofluorescence and all seven by immunoblotting. The serological examination of the entire herd revealed a high herd seroprevalence of antibodies directed against N. caninum. Potential modes of infection with N. caninum in the herd are discussed, in particular the possibility of exposure to a point source (definitive host).
authors.affiliation: Institut für epidemiologische Diagnostik der Bundesforschungsanstait für Viruskrankheiten der Tiere, Wusterhausen.
authors.forename: G
authors.initials: G
authors.lastname: Schares
authors.forename: M
authors.initials: M
authors.lastname: Peters
authors.forename: R
authors.initials: R
authors.lastname: Wurm
authors.forename: K
authors.initials: K
authors.lastname: Tackmann
authors.forename: K
authors.initials: K
authors.lastname: Henning
authors.forename: F J
authors.initials: FJ
authors.lastname: Conraths
mesh_terms: D000034:Abortion, Veterinary / Q000453:epidemiology; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases; D003048:Coccidiosis / Q000150:complications / Q000453:epidemiology / Q000662:veterinary*; D004196:Disease Outbreaks / Q000662:veterinary; D005260:Female; D005858:Germany / Q000453:epidemiology; D018688:Neospora / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*