[A method of using synergists in studying the level of activity of enzyme systems and their participation in the mechanism of action of insecticides on insects].
An indirect toxicology method was used to assess the monooxygenase (MO) and nonspecific esterase (NE) activities in three synanthropic cockroach species and four races of houseflies with the help of common and novel organophosphorus and pyrethroid synergists: piperonyl butoxide (PB), S,S,S-tributyltrithiophosphate (TBTP), Kitazin-P, Karesan, butylmercapto-2-thio-1,3,2-oxazaphosphorinan (AO-6), and 2-phenylmercapto-2-thio-1,3,2-oxazaphosphorinan (AO-2). The relationship between the coefficient of synergistic effect (CSE) and the concentrations of MO and NE inhibitors was described, and the minimum effective concentrations with the highest synergistic effect were determined. These concentrations are advisable for use in the insect enzyme assays and studies of insecticide detoxification and insect resistance to them.
Eremina
O Iu
OIu
Roslavtseva
S A
SA
D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000187:drug effects; D003058:Cockroaches / Q000187:drug effects / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006793:Houseflies / Q000187:drug effects / Q000201:enzymology*; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008294:Malathion / Q000494:pharmacology*; D010105:Oxygenases / Q000187:drug effects; D026023:Permethrin; D011722:Pyrethrins / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D007306:Insecticides; D011722:Pyrethrins; D026023:Permethrin; D010105:Oxygenases; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D008294:Malathion