title: Isolation of the major basic nuclear protein and its localization on chromosomes of the dinoflagellate, Oxyrrhis marina.
abstract: Basic nuclear proteins were extracted from isolated nuclei of Oxyrrhis marina. The HPLC pattern of the extract showed a single major peak, which consisted of a major band with an apparent molecular mass of 23 kDa on an SDS-PAGE gel. We designated this protein as Np23 because of its apparent molecular mass. The amino acid composition of this protein revealed its extremely basic nature with a high lysine content. Polyclonal antibodies were raised against Np23. Immunofluorescence microscopy showed that Np23 was localized within the nucleus of dividing and non-dividing cells as well, and immuno-electron microscopy showed that the protein was localized only on chromosomes. These data established that Np23 is the major basic chromosome protein of Oxyrrhis marina.
authors.affiliation: Institute of Natural Sciences, Nagoya City University, Japan.
authors.forename: K H
authors.initials: KH
authors.lastname: Kato
authors.forename: A
authors.initials: A
authors.lastname: Moriyama
authors.forename: P
authors.initials: P
authors.lastname: Huitorel
authors.forename: J
authors.initials: J
authors.lastname: Cosson
authors.forename: M
authors.initials: M
authors.lastname: Cachon
authors.forename: H
authors.initials: H
authors.lastname: Sato
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000737:chemistry* / Q000648:ultrastructure; D016054:DNA, Protozoan / Q000032:analysis; D004141:Dinoflagellida / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008239:Lysine / Q000032:analysis; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D016054:DNA, Protozoan; D009687:Nuclear Proteins; D008239:Lysine