Mapping of rat chromosome 2 markers generated from chromosome-sorted DNA.
A quantitative trait locus (QTL) for blood pressure has recently been mapped to a region of roughly 30 cM on rat Chromosome (Chr) 2 by linkage and by the use of congenic strains. For further fine mapping of the QTL, however, closely linked chromosome markers residing in this 30-cM region are required. In the current work, 36 new markers were generated by screening rat Chr 2-sorted DNA libraries and subsequently mapped using five F2 populations. Combining new and existing markers, the marker density for the 30-cM region approaches, on average, one marker per 1.1 cM.
Deng
A Y
AY
Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo 43699-0008, USA.
Jackson
C M
CM
Hoebee
B
B
Rapp
J P
JP
D000818:Animals; D001794:Blood Pressure / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D004247:DNA / Q000032:analysis; D005819:Genetic Markers; D005838:Genotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019655:Quantitative Trait, Heritable; D051381:Rats
D005819:Genetic Markers; D004247:DNA