Towards the development of a bioartificial pancreas: a 13C NMR study on the effects of alginate/poly-L-lysine/alginate entrapment on glucose metabolism by beta TC3 mouse insulinoma cells.
We have utilized 13C NMR spectroscopy to investigate glucose metabolism in the mouse insulinoma Beta TC3 cell line. Cells were cultured and examined both as monolayers and entrapped in alginate/poly-L-lysine/alginate beads. Entrapped cultures were tested at 3 and 30 days post-entrapment. The purpose of this study was to assess whether the entrapped environment affects glucose metabolism and insulin secretion. Both monolayer and entrapped cultures were fed with 10 mM [1-(13)C]-glucose for 4 hrs. prior to extraction with perchloric acid. Our data revealed that beta TC3 cells possess a reduced tricarboxylic acid (TCA) activity in the entrapment cultures, and that they metabolized pyruvate primarily via pyruvate dehydrogenase regardless of the mode or age of the culture.
Constantinidis
I
I
Frederik Philips Magnetic Resonance Research Center, Department of Radiology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Mukundan
N E
NE
Gamcsik
M P
MP
Sambanis
A
A
D000464:Alginates / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002247:Carbon Isotopes; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D005947:Glucose / Q000378:metabolism*; D020723:Glucuronic Acid; D006019:Glycolysis; D006603:Hexuronic Acids; D007340:Insulinoma / Q000188:drug therapy / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D019397:Pancreas, Artificial; D010472:Perchlorates / Q000737:chemistry; D011107:Polylysine / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000464:Alginates; D002247:Carbon Isotopes; D006603:Hexuronic Acids; D010472:Perchlorates; D011107:Polylysine; D019344:Lactic Acid; D020723:Glucuronic Acid; D005947:Glucose; D004801:Eosine Yellowish-(YS)