Fluorescence-based isolation of bacterial genes expressed within host cells.
A selection strategy was devised to identify bacterial genes preferentially expressed when a bacterium associates with its host cell. Fourteen Salmonella typhimurium genes, which were under the control of at least four independent regulatory circuits, were identified to be selectively induced in host macrophages. Four genes encode virulence factors, including a component of a type III secretory apparatus. This selection methodology should be generally applicable to the identification of genes from pathogenic organisms that are induced upon association with host cells or tissues.
Valdivia
R H
RH
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. valdivia@cmgm.stanford.edu
Falkow
S
S
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D005260:Female; D005434:Flow Cytometry; D005453:Fluorescence; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012486:Salmonella typhimurium / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D013154:Spleen / Q000382:microbiology; D014157:Transcription Factors / Q000235:genetics; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D008164:Luminescent Proteins; C079792:PhoQ protein, Bacteria; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C061064:PhoP protein, Bacteria; D049452:Green Fluorescent Proteins