title: PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes.
abstract: A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were determined, and in 144 of 149 cases (97%) the results were congruous. Strains of types that were not included in the panel of capture probes were emm genotyped by sequencing.
authors.affiliation: HRL, London, United Kingdom. nsaunder@phls.co.uk
authors.forename: N A
authors.initials: NA
authors.lastname: Saunders
authors.forename: G
authors.initials: G
authors.lastname: Hallas
authors.forename: E T
authors.initials: ET
authors.lastname: Gaworzewska
authors.forename: L
authors.initials: L
authors.lastname: Metherell
authors.forename: A
authors.initials: A
authors.lastname: Efstratiou
authors.forename: J V
authors.initials: JV
authors.lastname: Hookey
authors.forename: R C
authors.initials: RC
authors.lastname: George
mesh_terms: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D018070:Biological Specimen Banks; D002352:Carrier Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012015:Reference Standards; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013297:Streptococcus pyogenes / Q000145:classification*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C014689:streptococcal M protein