[Some immunochemical and immunobiological properties of Vibrio cholerae serovar 0139 capsule antigen].
Capsular antigen (C-antigen) was isolated from culture fluid of V. cholerae serovar 0139 strain P16064 4-fold fractionation by (NH4)2SO4 after Weinblatt (1980). Electrophoretic separation in PAAG-SDS revealed two protein subunits with molecular weights 38 +/- 2 and 61 +/- 2 KD in the C-antigen and whole-cell lysates of two strains (MO45 and P16064) of V. cholerae serovar 0139. Murine polyclonal anticapsular antibodies reacted with these protein subunits in immunoblotting, as well as with two rapidly migrating carbohydrate components. After proteinase treatment of the C-antigen and cell lysates from opaque colonies these antibodies helped detect two high-molecular zones in addition to rapidly migrating ones. C-antigen protects from death 75% of mice infected with V. cholerae 0.139 and 43% (P < 0.05) of those infected with V. cholerae eltor 230. The protective properties of C-antigen are due to lipopolysaccharide (LPS) but not to the protein component. The immunogenicity of LPS contained in the C-antigen is selective, whereas LPS isolated from biomass of V. cholerae 0139 cells possesses similar protective activity towards the homologous strain (49.5%) and the agent of Eltor cholera (50.0%).
Fedorova
V A
VA
Devdariani
Z L
ZL
Gromova
O V
OV
Livanova
L F
LF
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D051379:Mice; D014734:Vibrio cholerae / Q000276:immunology*
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines