Combined use of the "scratch and smear" sampling technique and Ultrafast Papanicolaou stain for intraoperative cytology.
OBJECTIVE
To assess the applicability of Ultrafast Papanicolaou stain (UFP), a 90-second, high-resolution stain incorporating the air-dried rehydration technique, to intraoperative cytology in surgical pathology laboratories.


METHODS
Two hundred sixty-two randomly selected surgical specimens for intraoperative consultations were included in the study. Each specimen was first conventionally prepared by touch imprinting, wet fixation in alcohol and staining by hematoxylin and eosin (H&E); the remaining specimens were then sampled by the "scratch and smear" technique and subjected to the UFP staining protocol.


RESULTS
The combined use of the scratch and smear sampling technique and UFP staining was superior to the conventional methods of touch imprinting and H&E staining in every case because of the following features: (1) intact tissue fragments composed of both epithelial and stromal elements rather than epithelial cells only, (2) polychromasia rather than bichromasia, (3) sharper nuclear and cytoplasmic details, (4) histologic criteria applicable to thick tissue fragments due to transparency and flatness and (5) clear background devoid of red blood cells and eosin.


CONCLUSIONS
The combined use of the "scratch and smear" sampling technique and UFP enhances intraoperative cytology.
Yang
G C
GC
Department of Pathology, New York University Medical Center, New York 10016, USA.
Hoda
S A
SA
D003584:Cytological Techniques; D005069:Evaluation Studies as Topic; D006801:Humans; D007432:Intraoperative Period / Q000379:methods; D009369:Neoplasms / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods