title: In vitro fertilization and preimplantation embryo development of African green monkeys (Cercopithecus aethiops).
abstract: Ovaries of five adult female African green monkeys were stimulated by repeated administrations of equine chorionic gonadotrophin (eCG), followed by a single administration of human chorionic gonadotrophin (hCG). Oocytes were collected from enlarged follicles 28 h after hCG administration and incubated in vitro for 288 h. Oocytes that had extruded the first polar body were inseminated with spermatozoa that had been incubated for 4 to 6 h in medium with caffeine and dibutyryl cyclic AMP. Of these oocytes, 66% were fertilized and the incidence of polyspermy was 37%. Eighty-two percent of the fertilized eggs cleaved, with some developing into expanded blastocysts.
authors.affiliation: Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Ibaraki, Japan. sankai@nih.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Sankai
authors.forename: F
authors.initials: F
authors.lastname: Cho
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D001755:Blastocyst; D002522:Chlorocebus aethiops / Q000196:embryology*; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D047108:Embryonic Development; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D010053:Ovary / Q000187:drug effects; D011247:Pregnancy
chemical_list: D006063:Chorionic Gonadotropin