Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost.
The genome of infectious salmon anemia virus (ISAV), which infects farmed Atlantic salmon (Salmo salar L.), is characterized here. The virus has an RNA genome, as shown by using specific DNA virus metabolic inhibitors and radioactive in vivo labeling of ISAV nucleic acid. Electrophoresis of [14C]uridine-labeled ISAV RNA revealed that the ISAV genome is segmented. The genome consists of eight segments that range from 1.0 to 2.3 kb, with a total molecular size of approximately 14.5 kb. One ISAV-specific molecular clone, corresponding to the smallest genome segment, was obtained by cDNA cloning of mRNA from an ISAV-infected cell culture. This clone gave a positive hybridization signal on Northern blots of pelleted ISAV. Pretreatment of the ISAV pellet with RNase A resulted in the disappearance of the positive hybridization signal, demonstrating that the genome is single stranded. Reverse transcriptase PCR with primers corresponding to sequences from the molecular clone and target RNA from ISAV-infected and noninfected fish tissues gave specific positive reactions. Alignments of the nucleotide sequence of the molecular clone did not reveal significant homology with any other available sequence in databases. However, the data presented here, together with morphological and replicational properties previously described, indicate that ISAV has a strong resemblance to members of the Orthomyxoviridae family. This is the first thoroughly characterized orthomyxo-like virus isolated from a teleost.
Mjaaland
S
S
National Veterinary Institute and Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, Oslo, Norway. Siri.Mjaaland@veths.no
Rimstad
E
E
Falk
K
K
Dannevig
B H
BH
D000740:Anemia / Q000662:veterinary* / Q000821:virology; D000818:Animals; D001267:Atlantic Ocean; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D005393:Fish Diseases; D016679:Genome, Viral; D008970:Molecular Weight; D009975:Orthomyxoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009976:Orthomyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000302:isolation & purification*; D012474:Salmon / Q000821:virology*
D012367:RNA, Viral