title: Histological and biomechanical analysis of bone and interface reactions around hydroxyapatite-coated intramedullary implants of different stiffness: a pilot study on the goat.
abstract: We hypothesized that reduced stem stiffness of orthopaedic implants contributes to a high risk of loosening, since interface stresses and relative motions may exceed a tolerable range. To study this hypothesis, three types of load-bearing implant with different stiffnesses were inserted into the tibia of the goat. Histological analysis was performed of bone repair after insertion of the implant, bone ingrowth, interface disruption and loosening. A finite element model of the configuration provided the quantitative range of interface stresses and relative motions for the present experiment. The implants were made out of stainless steel, hollow titanium and a thin titanium core covered with a polyacetal coating. The stiffness ratios of these implants were approximately 10:4:1, respectively. All implants were coated with a layer of hydroxyapatite (HA) in order to minimize the possible biological effects of the different implant materials. Irrespective of the type of implant, there was a repair phase that lasted 6-12 weeks. The stiff implants functioned well. Large areas of bone bonding to the HA layer were found after the repair phase at 12 weeks postoperatively. After 24 weeks, some signs of loosening were observed. More loosening occurred with the hollow titanium and polyacetal implants, mainly during the repair phase. Three hollow titanium and three polyacetal coated implants survived this period, and were killed after 24 weeks. The integrity of the HA layer at the bone-implant interface of the titanium implants was good. In the polyacetal implants, the repair reaction of the cortical bone was incomplete. Bone ingrowth into HA was largely lacking. In conclusion, we found significant differences in the repair and interface reactions around implants of different stiffness. Stiff implants showed favourable initial interface conditions for bone ingrowth. Intermediate and flexible implants provoked unfavourable interface conditions for initial bone ingrowth. The finite element study showed that the flexible stems produce larger micromotions and higher interface stresses at the bone-prosthesis interface than the stiff stems, indicating an explanation for the histological findings.
authors.affiliation: Institute of Orthopaedics, Orthopaedic Research Laboratory, University Hospital Nijmegen, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Buma
authors.forename: P J
authors.initials: PJ
authors.lastname: van Loon
authors.forename: H
authors.initials: H
authors.lastname: Versleyen
authors.forename: H
authors.initials: H
authors.lastname: Weinans
authors.forename: T J
authors.initials: TJ
authors.lastname: Slooff
authors.forename: K
authors.initials: K
authors.lastname: de Groot
authors.forename: R
authors.initials: R
authors.lastname: Huiskes
mesh_terms: D000080:Acetals; D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D016723:Bone Remodeling; D017886:Durapatite; D006041:Goats; D006622:Hip Prosthesis; D008422:Materials Testing; D008954:Models, Biological; D010865:Pilot Projects; D011108:Polymers; D011475:Prosthesis Failure; D013193:Stainless Steel; D013314:Stress, Mechanical; D013977:Tibia / Q000473:pathology / Q000601:surgery; D014025:Titanium
chemical_list: D000080:Acetals; D001672:Biocompatible Materials; D011108:Polymers; D013193:Stainless Steel; C016067:polyacetal; D017886:Durapatite; D014025:Titanium