Characterization of UT2 cells. The induction of peroxisomal 3-hydroxy-3-methylglutaryl-coenzyme a reductase.
In the liver 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is present not only in the endoplasmic reticulum but also in the peroxisomes. However, to date no information is available regarding the function of the peroxisomal HMG-CoA reductase in cholesterol/isoprenoid metabolism, and the structure of the peroxisomal HMG-CoA reductase has yet to be determined. We have identified a mammalian cell line that expresses only one HMG-CoA reductase protein and that is localized exclusively to peroxisomes. This cell line was obtained by growing UT2 cells (which lack the endoplasmic reticulum HMG-CoA reductase) in the absence of mevalonate. The cells exhibited a marked increase in a 90-kDa HMG-CoA reductase that was localized exclusively to peroxisomes. The wild type Chinese hamster ovary cells contain two HMG-CoA reductase proteins, the well characterized 97-kDa protein, localized in the endoplasmic reticulum, and a 90-kDa protein localized in peroxisomes. The UT2 cells grown in the absence of mevalonate containing the up-regulated peroxisomal HMG-CoA reductase are designated UT2*. A detailed characterization and analysis of this cell line is presented in this study.
Engfelt
W H
WH
Department of Biology, San Diego State University, San Diego, California 92182, USA.
Shackelford
J E
JE
Aboushadi
N
N
Jessani
N
N
Masuda
K
K
Paton
V G
VG
Keller
G A
GA
Krisans
S K
SK
D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D002457:Cell Extracts; D002458:Cell Fractionation; D002460:Cell Line; D002498:Centrifugation; D002999:Clone Cells; D006224:Cricetinae; D004790:Enzyme Induction; D006903:Hydroxymethylglutaryl CoA Reductases / Q000096:biosynthesis* / Q000276:immunology; D008099:Liver / Q000201:enzymology; D008297:Male; D008830:Microbodies / Q000201:enzymology*; D008856:Microscopy, Fluorescence; D051381:Rats; D017207:Rats, Sprague-Dawley
D002457:Cell Extracts; D006903:Hydroxymethylglutaryl CoA Reductases