Pepsinogen C gene product is a possible growth factor during gastric mucosal healing.
We isolated, by the subtraction cloning method, a pepsinogen C (PGC) gene fragment (the sequence between the 968th and 1179th base pairs) from a rat gastric mucosal cDNA library as a cDNA clone encoding a substance that promotes growth of the normal rat gastric mucosal cell line RGM1. Northern blot analysis revealed that PGC gene expression was enhanced not only in acetic acid-induced chronic gastric ulcers but also in indomethacin-induced gastric mucosal lesions. PGC gene expression was also increased in the Helicobacter felis-infected stomachs. Thus, the PGC gene may play a role in gastric epithelial cell growth during gastric mucosal healing.
Kishi
K
K
Department of Medicine, Kyoto University Graduate School of Medicine, Japan.
Kinoshita
Y
Y
Matsushima
Y
Y
Okada
A
A
Maekawa
T
T
Kawanami
C
C
Watanabe
N
N
Chiba
T
T
D019342:Acetic Acid; D000818:Animals; D018076:DNA, Complementary / Q000302:isolation & purification; D005753:Gastric Mucosa / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics; D005786:Gene Expression Regulation; D006133:Growth Substances / Q000235:genetics* / Q000502:physiology* / Q000627:therapeutic use; D016998:Helicobacter / Q000502:physiology; D008297:Male; D010435:Pepsinogens / Q000235:genetics* / Q000502:physiology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics
D018076:DNA, Complementary; D006133:Growth Substances; D010435:Pepsinogens; D012333:RNA, Messenger; D019342:Acetic Acid