Genes required for assembly and function of the protein synthetic system in Chlamydia trachomatis are expressed early in elementary to reticulate body transformation.
Following binding and internalization into the host cell cytoplasm, elementary bodies (EB) of the obligate intracellular bacterium Chlamydia trachomatis undergo a developmental process resulting in production of reticulate bodies (RB), the vegetative growth form of the organism. EB are metabolically inactive, but EB to RB transformation requires bacterial protein synthesis. Using HeLa cells infected with EB of C. trachomatis serovar C, we examined the time of first appearance of transcripts from several genes whose products are required for assembly and function of the chlamydial protein synthetic system. We monitored appearance of chlamydial RNAs using reverse transcription-polymerase chain reaction assays targeting primary transcripts from the bacterial rRNA operons, and mRNAs encoding the glycyl tRNA synthetase and the ribosomal proteins S5 and L5. Transcripts from the proximal rRNA promoters, and those from the r-protein and tRNA synthetase genes, are detectable as early as 4 h after EB-host binding; transcripts from distal rRNA promoters do not appear until 6 h post-infection. Thus, expression of bacterial genes whose products are required for protein synthesis begins earlier in chlamydial EB to RB development than previously thought.
Gérard
H C
HC
Department of Microbiology and Immunology, Allegheny University of the Health Sciences, MCP Hahnemann School of Medicine, Philadelphia, PA 19129, USA.
Whittum-Hudson
J A
JA
Hudson
A P
AP
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D005798:Genes, Bacterial; D006032:Glycine-tRNA Ligase / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction; D018272:Porins; D011401:Promoter Regions, Genetic
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018076:DNA, Complementary; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis; D006032:Glycine-tRNA Ligase