title: Chloroplastic prenylated proteins.
abstract: By in vivo [3H]mevalonate labelling of spinach combined with biochemical analysis, evidence is provided for the existence of protein prenylation in chloroplasts. Approximately 20 prenylated polypeptides were resolved by SDS-PAGE followed by autoradiography. Thermolysin treatment of intact chloroplasts revealed that about 40% of the prenylated polypeptides were associated with the cytoplasmic surface of the outer envelope membrane. The remaining portion was present in thylakoids and/or the inner envelope membrane. The majority of the prenylated polypeptides were associated with larger membrane protein complexes. A farnesyl protein transferase activity was found to be associated with the thylakoid membrane.
authors.affiliation: Department of Biochemistry, Stockholm University, Sweden. ingela@biokemi.su.se
authors.forename: I
authors.initials: I
authors.lastname: Parmryd
authors.forename: C A
authors.initials: CA
authors.lastname: Shipton
authors.forename: E
authors.initials: E
authors.lastname: Swiezewska
authors.forename: G
authors.initials: G
authors.lastname: Dallner
authors.forename: B
authors.initials: B
authors.lastname: Andersson
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D002498:Centrifugation; D011232:Chemical Precipitation; D002736:Chloroplasts / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008798:Mevalonic Acid / Q000493:pharmacokinetics; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D017368:Protein Prenylation; D018724:Spinacia oleracea; D013347:Subcellular Fractions; D014316:Tritium
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins; D014316:Tritium; D019883:Alkyl and Aryl Transferases; C064911:p21(ras) farnesyl-protein transferase; D008798:Mevalonic Acid