Competitive enzyme immunoassay for urinary vanillylmandelic acid.
An enzyme immunoassay for urinary vanillylmandelic acid (VMA) using polyclonal antiserum and VMA-acetylcholinesterase conjugate as enzymatic tracer is described. Two different strategies for immunogen preparation were developed and enantioselectivity was demonstrated. Selected EIA allowed direct measurement of urinary VMA using D(-)-VMA as standard with good sensitivity (MDC = 0.1 mumol/l) and precision (CV less than 7% in 0.2-2.25 mumol/l range). Cross-reactivity with homovanillic acid (HVA) was 0.8% and less than 0.4% with other structurally related catecholamine metabolites. Intra- and inter-assay repeatability were less than 10% and recovery was 97.3% +/- 3%. Good correlation was obtained for EIA and HPLC analysis with normal and pathologic human urine samples (EIA = 0.895 HPLC-7.085, r2 = 0.98, n = 47).
Taran
F
F
CEA, Service des Molécules Marquées DBCM, CEA-Saclay, Gif sur Yvette, France.
Bernard
H
H
Valleix
A
A
Créminon
C
C
Grassi
J
J
Olichon
D
D
Deverre
J R
JR
Pradelles
P
P
D000110:Acetylcholinesterase / Q000378:metabolism; D000906:Antibodies; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D014642:Vanilmandelic Acid / Q000652:urine*
D000906:Antibodies; D014642:Vanilmandelic Acid; D000110:Acetylcholinesterase