title: Immunologic correlates of protection against rotavirus challenge after intramuscular immunization of mice.
abstract: The capacity of intramuscular (i.m.) inoculation of mice with homologous or heterologous host rotaviruses to induce protection from challenge was evaluated. i.m. inoculation with live, wild-type rotavirus (murine strain EDIM) induced complete protection from viral shedding after challenge for at least 6 weeks after inoculation; protection was correlated with production of virus-specific immunoglobulin A (IgA) by lamina propria (LP) lymphocytes. i.m. inoculation with inactivated EDIM, cell culture-adapted EDIM, or simian strain RRV was associated with partial protection, characterized by reduced viral shedding after challenge. Partial protection after challenge was not associated with production of virus-specific IgA by LP lymphocytes. The mechanisms by which i.m. inoculation induces virus-specific humoral immune responses in the small intestinal LP were examined.
authors.affiliation: Division of Immunologic and Infectious Diseases, The Children's Hospital of Philadelphia, The University of Pennsylvania School of Medicine, 19104, USA. coffin@email.chop.edu
authors.forename: S E
authors.initials: SE
authors.lastname: Coffin
authors.forename: C A
authors.initials: CA
authors.lastname: Moser
authors.forename: S
authors.initials: S
authors.lastname: Cohen
authors.forename: H F
authors.initials: HF
authors.lastname: Clark
authors.forename: P A
authors.initials: PA
authors.lastname: Offit
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000032:analysis; D005243:Feces / Q000821:virology; D005260:Female; D005766:Gastrointestinal Contents / Q000382:microbiology; D007070:Immunoglobulin A / Q000096:biosynthesis; D007273:Injections, Intramuscular; D007420:Intestine, Large / Q000821:virology; D007421:Intestine, Small / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral / Q000096:biosynthesis*; D012401:Rotavirus / Q000276:immunology* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000276:immunology* / Q000517:prevention & control; D013997:Time Factors; D014765:Viral Vaccines / Q000008:administration & dosage*; D017201:Virus Shedding
chemical_list: D000956:Antigens, Viral; D007070:Immunoglobulin A; D012367:RNA, Viral; D014765:Viral Vaccines