Identification of a genomic locus containing three slow myosin heavy chain genes in the chicken.
Two unique cDNA clones containing chicken slow myosin heavy chain (MyHC) inserts have been isolated from an expression library. Immunochemical analyses of the expressed proteins using different slow MyHC specific monoclonal antibodies were consistent with the two clones encoding slow MyHC 1 (SM1) and slow MyHC 2 (SM2) protein sequences. Northern blot analyses showed that the clones hybridized with 6-kb mRNAs that are differentially expressed in developing and adult slow muscles, further supporting the conclusion that these two clones represent SM1 and SM2 cDNAs. Sequence analyses show that both clones encode the highly conserved light meromyosin portion of the sarcomeric myosin rod and are 78-81% homologous to a mammalian slow/cardiac beta-MyHC cDNA. Hybridization using PCR generated probes specific for SM1 and SM2 sequences demonstrated that the genes encoding these two slow MyHCs colocalized to an 80-kb BssHII genomic fragment. We further show that a probe specific to a third slow MyHC gene also hybridized with the same 80-kb genomic fragment. We conclude that in the chicken genome there is a slow MyHC locus containing at least three distinct slow MyHC genes.
Chen
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Department of Food Science and Technology, University of California at Davis, 95616, USA.
Moore
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Wick
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Bandman
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D000595:Amino Acid Sequence; D000818:Animals; D024743:Atrial Myosins; D030161:Avian Proteins; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D015723:Gene Library; D006321:Heart / Q000196:embryology; D008969:Molecular Sequence Data; D005810:Multigene Family; D009124:Muscle Proteins / Q000235:genetics; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics*; D015879:Myosin Subfragments / Q000235:genetics; D009218:Myosins / Q000235:genetics; D016415:Sequence Alignment
D030161:Avian Proteins; D018076:DNA, Complementary; D009124:Muscle Proteins; D015879:Myosin Subfragments; C071555:VMHC1 protein, Gallus gallus; D024743:Atrial Myosins; C094463:AMHC1 protein, chicken; D018995:Myosin Heavy Chains; D009218:Myosins