title: [Cooling of boar spermatozoa prior to freezing and post thaw quality and evaluation of membrane state using chlortetracycline (CTC) staining].
abstract: The influence of an extended holding time at room temperature (+18 degrees C) before freezing on boar sperm quality was investigated. 17 ejaculates were collected from 5 different boars by separation in sperm rich and sperm poor fraction. The ejaculate were split, diluted 1+1 with Merck I-Medium, and submitted to three different treatments before freezing: 1. Sperm rich fraction, cooling to +20 degrees C for 1.5 h and subsequent cooling to +15 degrees C for 2.5 h; 2. Sperm rich fraction, cooling to +18 degrees C for 4 h and subsequent holding time at +18 degrees C for 16 h; 3. Whole ejaculate (sperm rich fraction plus seminal plasma), cooling to +18 degrees C for 4 h and subsequent holding time at +18 degrees C for 16 h. Subjectively assessed post thaw motility (SMOT), computer-measured motility (CMOT), and acrosome integrity (NAR), assessed by phase contrast microscopy were significantly (p < 0.05) higher after extended holding time (procedure 2 and 3) compared to short holding time (procedure 1). The exposure to seminal plasma during holding had no significant effect. Chlortetracyclin (CTC) staining of sperm membranes gave no reliable information in the presence of an EDTA-containing preservation medium, used routinely in the preservation process.
authors.affiliation: Institut für Reproduktionsmedizin, Tierärztliche Hochschule Hannover.
authors.forename: E
authors.initials: E
authors.lastname: Kotzias-Bandeira
authors.forename: D
authors.initials: D
authors.lastname: Waberski
authors.forename: K F
authors.initials: KF
authors.lastname: Weitze
mesh_terms: D000177:Acrosome / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002751:Chlortetracycline; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004492:Edetic Acid; D004542:Ejaculation; D008297:Male; D012661:Semen / Q000166:cytology / Q000378:metabolism; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*; D013552:Swine
chemical_list: D004492:Edetic Acid; D002751:Chlortetracycline