Oxidation of ferric-cytochrome c by N, N'-bis (2-hydroperoxy-2-methoxyethyl)-1,4,5,8-naphthalene tetracarboxylic diimide, NP III--site specific modification of cytochrome c.
When the synthetic hydroxyl radical generator, N, N'-bis (2-Hydroperoxy-2-methoxyethyl)-1,4,5,8-naphthalene-tetracarboxylic diimide, NP III, was photoirradiated in the presence of ferric-cytochrome c (Fe3+), the fragmentation peak corresponding to the specific oxidative modification at the histidine site of cytochrome c was observed in the Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF) measurement. Without photoirradiation, no such protein oxidation was observed in the experimental conditions employed. This result suggests the possible usage of NP-III as an oxidative protein-modifying reagent.
Matsugo
S
S
Department of Chemical & Biochemical Engineering, Faculty of Engineering, Toyama University, Japan.
Yasui
F
F
Konishi
T
T
D001665:Binding Sites; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000378:metabolism; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D006639:Histidine / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D003497:Cyclic N-Oxides; D003574:Cytochrome c Group; D009281:Naphthalenes; C067580:N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic diimide; D017665:Hydroxyl Radical; D006639:Histidine; C017245:5,5-dimethyl-1-pyrroline-1-oxide