Cloning and characterization of a murine platelet-activating factor receptor gene.
A murine gene encoding a platelet-activating factor receptor (PAFR) was cloned. The gene was mapped to a distal region of chromosome 4 both by fluorescence in situ hybridization and by molecular linkage analysis. Northern blot analysis showed a high expression of the PAFR message in peritoneal macrophages. When C3H/HeN macrophages were treated with bacterial lipopolysaccharide (LPS) or synthetic lipid A, the PAFR gene expression was induced. Bacterial LPS, but not lipid A, induced the level of PAFR mRNA in LPS-unresponsive C3H/HeJ macrophages. These induction patterns were parallel to those of tumor necrosis factor-alpha mRNA. Thus, the PAFR in macrophages plays important roles in LPS-induced pathologies.
Ishii
S
S
Department of Biochemistry, Faculty of Medicine, University of Tokyo.
Nakamura
M
M
Waga
I
I
Kume
K
K
Izumi
T
T
Shimizu
T
T
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D015723:Gene Library; D005796:Genes; D006168:Guinea Pigs; D008050:Lipid A / Q000138:chemical synthesis / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D051379:Mice / Q000235:genetics*; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009115:Muridae / Q000235:genetics; D009928:Organ Specificity; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
D008050:Lipid A; D008070:Lipopolysaccharides; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor