title: Measurement of catecholamines in mouse bone marrow by means of HPLC with electrochemical detection.
abstract: OBJECTIVE
  Noradrenergic innervation is present in the bone marrow and adrenergic agents can modulate hematopoiesis. However, since no data are available concerning endogenous catecholamines at this level, we investigated their presence and origin.
  
  
  METHODS
  Using a high performance liquid chromatographic method, we have and measured endogenous catecholamines in bone marrow from normal, 6-OHDA-treated and pargyline-treated mice.
  
  
  RESULTS
  Noradrenaline, adrenaline and dopamine levels were, respectively, 2806.74 +/- 408.85, 803.37 +/- 87.66 and 274.47 +/- 51.54 pg/g of tissue. Noradrenaline levels were lower after 6-OHDA (1130.47 +/- 142.73 pg/g of tissue, p < 0.01 vs. control values) and higher after pargyline (4122.62 +/- 509.54 pg/g of tissue, p < 0.05). None of these treatments significantly affected adrenaline or dopamine content.
  
  
  CONCLUSIONS
  Noradrenaline in the bone marrow originates mainly from sympathetic nerve endings and is metabolized through specific enzymatic pathways. Adrenaline and dopamine may originate from other sources, such as the systemic circulation.
authors.affiliation: Department of Internal Medicine and Therapeutics, II Faculty of Medicine, University of Pavia, Varese, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Marino
authors.forename: M
authors.initials: M
authors.lastname: Cosentino
authors.forename: R
authors.initials: R
authors.lastname: Bombelli
authors.forename: M
authors.initials: M
authors.lastname: Ferrari
authors.forename: G J
authors.initials: GJ
authors.lastname: Maestroni
authors.forename: A
authors.initials: A
authors.lastname: Conti
authors.forename: S
authors.initials: S
authors.lastname: Lecchini
authors.forename: G
authors.initials: G
authors.lastname: Frigo
mesh_terms: D018663:Adrenergic Agents / Q000008:administration & dosage*; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D016627:Oxidopamine / Q000008:administration & dosage*; D010293:Pargyline / Q000008:administration & dosage*
chemical_list: D018663:Adrenergic Agents; D002395:Catecholamines; D016627:Oxidopamine; D010293:Pargyline