Deuterium distribution in lactate as tracer of metabolic pathways.
2H-NMR spectroscopy has been used to study the distribution of 2H atoms in brain lactate, after intraperitoneal injection of 2H2O. Information on brain metabolism was obtained from the ratio [(3-2H) lactate]/[(2-2H)-lactate] which was found very sensitive to the PO2 in the breathing air. The dependence of this ratio on the rates of metabolic pathways is an example of the possibility to use the distribution of 2H atoms in a molecule to probe metabolic flow through different pathways.
Vianello
F
F
Department of Biological Chemistry, University of Padua, Italy.
Scarpa
M
M
Viglino
P
P
Rigo
A
A
D000388:Air; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003903:Deuterium; D017666:Deuterium Oxide; D007700:Kinetics; D007773:Lactates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010100:Oxygen / Q000032:analysis; D010313:Partial Pressure; D051381:Rats; D017208:Rats, Wistar; D012119:Respiration
D007773:Lactates; D003903:Deuterium; D017666:Deuterium Oxide; D010100:Oxygen