title: Developmental regulation of a plant encoded inhibitor of eukaryotic initiation factor 2 alpha phosphorylation.
abstract: An inhibitor of eIF-2a phosphorylation was identified in various plant species. The plant protein (termed PKI) specifically cross-reacts with monoclonal antiserum that recognizes the glycosylated, active form of a M(r) 87 kD protein analog (p67) from reticulocytes. Northern blot analysis using a probe to the reticulocyte inhibitor cDNA further supports the presence of analogous transcripts in plant tissue. PKI specifically inhibits the phosphorylation of the plant encoded eIF-2 alpha kinase (pPKR) as well as plant and human eIF-2 alpha phosphorylation. The interaction between PKI and pPKR is indicated by their copurification on dsRNA agarose, despite evidence showing that PKI does not bind dsRNA. Further, wheat PKI inhibits human PKR phosphorylation but activity is recovered by immuno-depletion of PKI from wheat germ fractions. PKI is temporally regulated during plant growth and development. It is maximally present in extracts from dormant seeds, however, it is not detectable soon after leaf emergence at approximately 48 h post-imbibition. PKI levels are again detectable at the mid-milk stage in seed development. Protein levels of pPKR in ribosomal salt wash and cytosolic extracts from healthy plant tissue remain essentially constant throughout the life cycle. In contrast, pPKR activity levels based upon autophosphorylation vary significantly and are inversely correlated with PKI protein levels. Phosphorylation of eIF-2 alpha is a classical mechanism for the downregulation of protein synthesis suggesting that inhibition of pPKR activity by PKI may contribute to the dramatic and rapid increase in protein synthesis observed during seed germination.
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie 82071, USA.
authors.forename: J O
authors.initials: JO
authors.lastname: Langland
authors.forename: L
authors.initials: L
authors.lastname: Langland
authors.forename: C
authors.initials: C
authors.lastname: Zeman
authors.forename: D
authors.initials: D
authors.lastname: Saha
authors.forename: D A
authors.initials: DA
authors.lastname: Roth
mesh_terms: D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D015852:Eukaryotic Initiation Factor-2 / Q000037:antagonists & inhibitors*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D006031:Glycosylation; D006801:Humans; D010766:Phosphorylation; D018515:Plant Leaves; D010940:Plant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors; D012156:Reticulocytes / Q000378:metabolism; D019892:eIF-2 Kinase
chemical_list: D000911:Antibodies, Monoclonal; D015852:Eukaryotic Initiation Factor-2; D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases; D019892:eIF-2 Kinase