Expression and modulation of the Lewis x antigen (CD15) on the T cell line Molt-4.
The T cell lines Molt-4 and H9 exhibited a characteristic distribution of the cell adhesion molecule Lewis x (CD15, lacto-N-fucopentanose III) showing an unusually broad peak by flow cytometry ranging from cells without CD15 to cells with high expression. The cytokines IL-1, IL-2, IFN-beta, IFN-gamma, and TNF-alpha, known to activate T cells, did not affect CD15 expression. However, phorbol myristate acetate and the thymic peptide extract Thymex-L were able to enhance both the number of CD15-positive cells and the median fluorescence. The effects of both inducers were dose- and time-dependent. An additive or synergistic effect was not seen. These data suggest that phorbol esters and distinct thymic peptides can modulate the expression of the cell surface antigen CD15.
Schulze-Forster
K
K
Institut für Pharmazie der Freien Universität Berlin, Abteilung Pharmazeutische Biochemie, Molekularbiologie und Biotechnologie, Germany.
Maurer
H R
HR
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D002452:Cell Count / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology; D005434:Flow Cytometry; D005453:Fluorescence; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D015458:Leukemia, T-Cell; D016256:Lewis X Antigen / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013949:Thymus Extracts / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D016207:Cytokines; D016256:Lewis X Antigen; D013949:Thymus Extracts; C092820:Thymex-L; D013755:Tetradecanoylphorbol Acetate