Identification of soluble binding proteins for an insect neuropeptide.
A photoaffinity analog of Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez-PBAN) was used to identify PBAN binding proteins in various tissues of the corn earworm moth, H. zea. Synthetic Hez-PBAN was derivatized on Lys-27 with p-benzoyldihydrocinnamoyl-N-hydroxysuccinimide ester (BZDC-NHS ester). The resulting BZDC-PBAN stimulated pheromone production in H. zea isolated abdomens at levels comparable to those of the unmodified peptide. Photoaffinity labeling experiments using [3H]BZDC-PBAN with female moth tissues revealed soluble 100 and 115 kDa proteins in the brain-subesophageal ganglia complex, ventral nerve cord, and thoracic muscle that were specifically labeled with the PBAN analog.
Elliott
J T
JT
Department of Physiology and Biophysics, The University at Stony Brook, New York 11794-8661, USA.
Jurenka
R A
RA
Prestwich
G D
GD
Roelofs
W L
WL
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D019476:Insect Proteins / Q000096:biosynthesis / Q000378:metabolism* / Q000502:physiology; D008969:Molecular Sequence Data; D009036:Moths; D009479:Neuropeptides / Q000096:biosynthesis / Q000378:metabolism* / Q000502:physiology; D010779:Photofluorography; D012724:Sex Attractants / Q000096:biosynthesis / Q000378:metabolism* / Q000502:physiology
D000345:Affinity Labels; D002352:Carrier Proteins; D019476:Insect Proteins; D009479:Neuropeptides; D012724:Sex Attractants; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea