title: Increased sialyl Lewis A expression and fucosyltransferase activity with acquisition of a high metastatic capacity in a colon cancer cell line.
abstract: A human colon cancer cell line, OCUC-LM1(LM), was established from a liver metastasis in our laboratory. Intrasplenic injection of LM into nude mice was repeated three and five times, and the daughter cell lines were designated as LM-H3 and LM-H5 respectively. The level of sialyl Lewis A (SLA) in the supernatant of LM-H3 and LM-H5 was 3 and 4.5 times higher than that of LM respectively. Flow cytometric analysis of SLA expression showed that the peak channel for LM was 113; for LM-H3, 126; and for LM-H5, 146. The mean fluorescence intensity of LM was 102.3 +/- 43.5; for LM-H3, 126.2 +/- 28.4; and for LM-H5, 144.8 +/- 23.4. In endothelial cell adhesion assays, the percentages of adherent LM-H3 and LM-H5 cells were significantly higher than for LM. The activity of alpha1-->4 fucosyltransferase was higher in LM-H3 and LM-H5 than in LM, but there was no difference in alpha2-->3 sialyltransferase activities for type 1 chain among the cell lines. Our results suggest that SLA expression is associated with acquisition of a high capacity for liver metastasis of colon cancer; increased SLA expression is due mainly to increased fucosyltransferase activity.
authors.affiliation: The First Department of Surgery, Osaka City University Medical School, Osaka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yamada
authors.forename: Y S
authors.initials: YS
authors.lastname: Chung
authors.forename: S
authors.initials: S
authors.lastname: Takatsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Arimoto
authors.forename: T
authors.initials: T
authors.lastname: Sawada
authors.forename: T
authors.initials: T
authors.lastname: Dohi
authors.forename: M
authors.initials: M
authors.lastname: Sowa
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D019040:E-Selectin / Q000276:immunology / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology; D005434:Flow Cytometry; D005647:Fucosyltransferases / Q000378:metabolism*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000096:biosynthesis; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D000080506:Sialyl Lewis X Antigen; D012799:Sialyltransferases / Q000378:metabolism; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D000906:Antibodies; D019040:E-Selectin; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D005647:Fucosyltransferases; D012799:Sialyltransferases; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase