Percutaneous penetration of N-nitroso-N-methyldodecylamine through human skin in vitro: application from cosmetic vehicles.
The human skin penetration of N-nitroso-N-methyldodecylamine (NDOMA) from isopropyl myristate (IPM) and two vehicles representative of cosmetic/personal care formulations was determined in vitro. When applied as an infinite dose in IPM (1 microgram/microliter) the average total absorption over 48 hr was 0.10 +/- 0.01% of the applied dose (all data are expressed as means +/- SE). When applied as a finite dose in a representative oil-in-water emulsion formulation the average total absorption over 48 hr was 4.66 +/- 0.76% of the applied dose. When applied as a finite dose in a representative shampoo formulation for 10 min, followed by rinsing (to represent in-use exposure conditions), the average total absorption over 48 hr was 0.75 +/- 0.17% of the applied dose. Approximately 72% of the NDOMA in the applied shampoo formulation was removed by rinsing. The overall data indicated that NDOMA could penetrate the skin but that penetration was low. The rate and extent of absorption, however, could be affected by differences in the vehicle of application, time of exposure and whether the formulation is (and the conditions are designed to mimic) a rinse-off or leave-on product.
Walters
K A
KA
An-eX analytical services Ltd., Cardiff.
Brain
K R
KR
Dressler
W E
WE
Green
D M
DM
Howes
D
D
James
V J
VJ
Kelling
C K
CK
Watkinson
A C
AC
Gettings
S D
SD
D002273:Carcinogens / Q000493:pharmacokinetics*; D003358:Cosmetics; D004655:Emulsions; D005260:Female; D006801:Humans; D008744:Methylamines / Q000493:pharmacokinetics*; D009226:Myristates / Q000494:pharmacology; D009602:Nitrosamines / Q000493:pharmacokinetics*; D012869:Skin Absorption / Q000187:drug effects; D017592:Skin Care
D002273:Carcinogens; D003358:Cosmetics; D004655:Emulsions; D008744:Methylamines; D009226:Myristates; C108285:N-nitroso-N-methyldodecylamine; D009602:Nitrosamines; C008205:isopropyl myristate