Evaluation of the AMPLICOR CMV test for direct detection of cytomegalovirus in plasma specimens.
We evaluated the AMPLICOR CMV test (PCR) for the direct detection of cytomegalovirus in plasma. Sixty-eight specimens were involved for the comparison between the AMPLICOR test and the antigenemia assay. The sensitivities, specificities, and positive and negative predictive values were 97.1, 100, 100, and 97.1%, respectively, for the AMPLICOR test and 79.4, 100, 100, and 82.9%, respectively, for the antigenemia assay.
Hiyoshi
M
M
Department of Laboratory Medicine, Osaka City University Medical School, Osaka, Japan. mh1@msic.med.osaka-cu.ac.jp
Tagawa
S
S
Takubo
T
T
Tanaka
K
K
Nakao
T
T
Higeno
Y
Y
Tamura
K
K
Shimaoka
M
M
Fujii
A
A
Higashihata
M
M
Yasui
Y
Y
Kim
T
T
Hiraoka
A
A
Tatsumi
N
N
D000956:Antigens, Viral / Q000097:blood; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000097:blood*; D005069:Evaluation Studies as Topic; D005260:Female; D006304:Health Status; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic
D000956:Antigens, Viral; D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic