Rapid typing and elucidation of new secondary metabolites of intact cyanobacteria using MALDI-TOF mass spectrometry.
Toxic cyanobacterial blooms are a threat because of secondary metabolite production. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identify intact microorganisms. Microgram quantities of prepared cells, including solvent (acetonitrile and ethanol) and alpha-cyano-4-hydroxycinnamic acid matrix, display spectra showing predominantly the secondary metabolites including known microcystins, micropeptin, and anabaenopeptolin. A new cyclic anabaenopeptolin has been identified using the Post-Source-Decay mode. Strains of various origins can easily be typed according to their cyclic peptide production, and toxic and nontoxic algal blooms can be differentiated within minutes.
Erhard
M
M
Institute of Biochemistry and Molecular Biology, Technical University Berlin, Germany.
von Döhren
H
H
Jungblut
P
P
D017033:Anabaena / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis*; D000458:Cyanobacteria / Q000378:metabolism*; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin