Lipids closely associated with bone morphogenetic protein (BMP)--and induced heterotopic bone formation. With preliminary observations of deficiencies in lipid and osteoinduction in lathyrism in rats.
An extensive literature on bone morphogenetic protein (BMP) induced generation and regeneration shows general agreement about one observation. The incidence and quantity of bone were greatest when BMP was delivered with a carrier of various biologic and non biologic polymers. In the present research, neutral lipids either endogenous in demineralized bone matrix (DBM) or exogenous in orign were employed as a delivery system for induced heterotopic bone formation in the rectus muscle in rats. Total neutral lipids including cholesterol were measured by correlated gravimetric and Sudan Black B dye binding methods. The heterotopic bone was measured by computer assisted radiomorphometric and histologic methods. Bone formation was measured by total calcium, DNA-P, and alkaline phosphatase activity. Composites of BMP and neutral lipids, separable from phospholipids by extraction with absolute acetone, were consistently osteoinductive. A significant quantity of the total bone lipid was closely associated with and extractable from the bone matrix non-collagenous protein fraction which had high levels of BMP activity. Lathyritic matrix was very low both in dye binding and osteoinductive activity. These observations suggest the possibility that lipids may serve as a BMP carrier in the process of induced bone development.
Urist
M R
MR
University of California, Los Angeles, School of Medicine, Department of Orthopaedic Surgery 90024, USA.
Behnam
K
K
Kerendi
F
F
Raskin
K
K
Nuygen
T D
TD
Shamie
A N
AN
Malinin
T I
TI
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D001857:Bone Matrix / Q000737:chemistry / Q000637:transplantation; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002113:Calcification, Physiologic; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D005612:Freeze Drying; D005780:Gelatin / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000502:physiology*; D009132:Muscles; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D016042:Transplantation, Heterotopic
D019485:Bone Morphogenetic Proteins; D005227:Fatty Acids; D008055:Lipids; D011994:Recombinant Proteins; D005780:Gelatin; D000469:Alkaline Phosphatase