title: Modulation by extracellular Cl- of volume-activated organic osmolyte and halide permeabilities in HeLa cells.
abstract: Organic osmolyte and halide permeability pathways activated in epithelial HeLa cells by osmotically induced cell swelling were studied using electrophysiological and radiotracer efflux techniques. On hypotonic challenge, HeLa cells responded by activating an efflux pathway for [3H]taurine and a swelling-induced outwardly rectifying Cl- channel. Removal of extracellular Cl-, or its replacement by a less permeable anion, enhanced taurine efflux and decreased the inward current (Cl- efflux). The effect of Cl- removal on taurine efflux was not a consequence of changes in membrane potential. The degree of deactivation of the Cl- current at depolarized potentials was also Cl- dependent, suggesting that external Cl- is necessary for channel activity. The Cl- channel inhibitors 1,9-dideoxyforskolin, tamoxifen, and 4,4'- diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) inhibited swelling-activated taurine efflux, with DIDS being the most potent, at variance with the sensitivity of the Cl- channel. DIDS effect was dependent on external Cl-; concentrations of DIDS that inhibited 50% of taurine efflux were 0.2 and 4 microM at low and high Cl-, respectively. The results could be interpreted on the basis of separate pathways for swelling-activated taurine efflux and Cl- current differentially affected by Cl-. Alternatively, taurine and Cl- flux might occur through a common channel, with the two solutes interacting within the pore and being affected differentially by Cl- replacement.
authors.affiliation: Departamento de Medicina Experimental, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: A
authors.initials: A
authors.lastname: Stutzin
authors.forename: A L
authors.initials: AL
authors.lastname: Eguiguren
authors.forename: L P
authors.initials: LP
authors.lastname: Cid
authors.forename: F V
authors.initials: FV
authors.lastname: Sepúlveda
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism* / Q000494:pharmacology*; D005576:Colforsin / Q000031:analogs & derivatives / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D009994:Osmolar Concentration; D011865:Radioisotope Dilution Technique; D013629:Tamoxifen / Q000494:pharmacology; D013654:Taurine / Q000378:metabolism*; D014316:Tritium; D014882:Water-Electrolyte Balance
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D013629:Tamoxifen; D014316:Tritium; D013654:Taurine; D005576:Colforsin; C047983:1,9-dideoxyforskolin; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid