High-performance liquid chromatographic determination of 4-methylpyrazole in plasma and in dialysate.
A rapid method for the determination of 4-methylpyrazole (4-MP) levels in plasma and in dialysate by isocratic reversed-phase high-performance liquid chromatography with UV detection is described. The internal standard was the 3-methylpyrazole (3-MP). Plasma sample preparation consisted of a protein precipitation. Dialysate samples were injected without preparation. The method was linear up to 30 mg l(-1) in plasma and up to 5 mg l(-1) in dialysate. The within-day precisions (C.V.) were less than 4% in plasma and were less than 2% in dialysate. The day-to-day precisions (C.V.) were less than 7% in plasma and were less than 3% in dialysate. This method is easy to perform and has practical interest for clinicians who need to monitor in emergency 4-MP levels in ethylene glycol and methanol poisonings.
Jobard
E
E
Laboratoire de Pharmacologie et Toxicologie, C.H.U., Angers, France.
Turcant
A
A
Harry
P
P
Le Bouil
A
A
Allain
P
P
D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors*; D002851:Chromatography, High Pressure Liquid; D015314:Dialysis Solutions / Q000032:analysis*; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000506:poisoning; D000077604:Fomepizole; D006801:Humans; D011041:Poisoning / Q000188:drug therapy; D011720:Pyrazoles / Q000032:analysis / Q000097:blood* / Q000627:therapeutic use; D006435:Renal Dialysis; D013056:Spectrophotometry, Ultraviolet
D015314:Dialysis Solutions; D005026:Ethylene Glycols; D011720:Pyrazoles; D000077604:Fomepizole; D000426:Alcohol Dehydrogenase; D019855:Ethylene Glycol