title: Influence of stimulation frequency on [Na+]i and contractile function in Langendorff-perfused rat heart.
abstract: To study the relationship between stimulation frequency and intracellular Na+ concentration ([Na+]i), Langendorff-perfused rat hearts were loaded with the Na(+)-sensitive dye sodium-binding benzofuran isophthalate (SBFI). An intracellular calibration procedure allowed SBFI fluorescence to be transformed into [Na+]i. Compartmentation of SBFI was evaluated by permeabilizing sarcolemmal membranes with saponin and subcellular compartments with Triton X-100. Most of the indicator was located in the myoplasm (69%). When stimulation frequency was increased from 0 to 6 Hz, [Na+]i increased from 3.0 to 7.9 mM, whereas pressure amplitude (PA) declined to 49% of the maximum recorded at 2 Hz. Blocking sarcoplasmic reticulum (SR) Ca2+ uptake with 2,5-di(tert-butyl)-1,4-benzohydroquinone (TBQ; 10 microM) at 2 Hz increased [Na+]i from 4.9 to 8.4 mM and decreased PA by 70%. Raising stimulation frequency then resulted in a further increase in [Na+]i and decline in PA. In conclusion, these data indicate that the rat heart is characterized by a negative pressure-frequency relationship associated with increasing [Na+]i at higher heart rates. After inhibition of SR Ca2+ uptake, [Na+]i further increases, whereas PA declines with increasing stimulus frequency. It is suggested that part of the rise of [Na+]i with stimulus frequency and TBQ may be associated with increased Ca2+ extrusion and Na+ influx on the Na+/Ca2+ exchange system.
authors.affiliation: Medizinische Klinik III, Universität Freiburg, Germany. maierl@ruf.uni-freiburg.de
authors.forename: L S
authors.initials: LS
authors.lastname: Maier
authors.forename: B
authors.initials: B
authors.lastname: Pieske
authors.forename: D G
authors.initials: DG
authors.lastname: Allen
mesh_terms: D000200:Action Potentials; D000818:Animals; D001572:Benzofurans; D001794:Blood Pressure / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002352:Carrier Proteins / Q000378:metabolism; D004558:Electric Stimulation; D004988:Ethers, Cyclic; D005260:Female; D005456:Fluorescent Dyes; D006321:Heart / Q000502:physiology*; D006873:Hydroquinones / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000502:physiology; D012964:Sodium / Q000378:metabolism*; D019831:Sodium-Calcium Exchanger; D013997:Time Factors
chemical_list: D001572:Benzofurans; D002352:Carrier Proteins; D004988:Ethers, Cyclic; D005456:Fluorescent Dyes; D006873:Hydroquinones; D019831:Sodium-Calcium Exchanger; C061665:sodium-binding benzofuran isophthalate; C019359:2,5-di-tert-butylhydroquinone; D012964:Sodium; D000252:Calcium-Transporting ATPases; D002118:Calcium