title: Sth132I, a novel class-IIS restriction endonuclease of Streptococcus thermophilus ST132.
abstract: The Sth132I restriction endonuclease (R.Sth132I) was detected in Streptococcus thermophilus ST132 and purified to near homogeneity by heparin Sepharose CL-6B affinity chromatography. Fragments from Sth132I digestion of plasmid DNA were subcloned into pUC19 in Escherichia coli DH5alpha and sequenced. Sequence analysis of inserts and their ligation junction sites revealed that Sth132I is a novel class-IIS restriction endonuclease, which recognizes the non-palindromic sequence 5'-CCCG(N)4-3', 3'-GGGC(N) 8-5'.
authors.affiliation: U.S. Department of Agriculture, ARS, Eastern Regional Research Center, Wyndmoor, PA 19038, USA.
authors.forename: M T
authors.initials: MT
authors.lastname: Poch
authors.forename: G A
authors.initials: GA
authors.lastname: Somkuti
authors.forename: D K
authors.initials: DK
authors.lastname: Solaiman
mesh_terms: D001483:Base Sequence; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013291:Streptococcus / Q000201:enzymology*
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific