Characterization of a putative retroviral env-related human protein.
In this study, we developed a rabbit polyclonal antibody to a fusion protein containing the envelope (env) transmembrane (TM) region from the human endogenous retroviral family, HERV-E. We used this reagent to document the expression of TM-related protein by Western blot in endothelial, colon and prostate carcinoma and seminoma cell lines and in peripheral blood mononuclear cells from a healthy donor. We detected a 58-kD protein (as compared to murine TM of 15 kD) that had specificity for the env-related antibodies of the polyclonal antiserum.
Turbeville
M A
MA
Department of Pathology, University of Cincinnati College of Medicine, Ohio, USA.
Rhodes
J C
JC
Hyams
D M
DM
Distler
C M
CM
Steele
P E
PE
D000230:Adenocarcinoma / Q000737:chemistry; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D003110:Colonic Neoplasms / Q000737:chemistry; D004727:Endothelium / Q000737:chemistry; D015686:Gene Products, env / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins; D009369:Neoplasms / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000737:chemistry; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000276:immunology; D012190:Retroviridae; D012191:Retroviridae Proteins / Q000032:analysis* / Q000276:immunology; D018239:Seminoma / Q000737:chemistry; D013736:Testicular Neoplasms / Q000737:chemistry; D014407:Tumor Cells, Cultured / Q000737:chemistry; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000276:immunology
D015686:Gene Products, env; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins; C075727:retroviral p15E-related protein, human