title: Sequence of the Bacillus subtilis genome region in the vicinity of the lev operon reveals two new extracytoplasmic function RNA polymerase sigma factors SigV and SigZ.
abstract: Two regions with sizes 18,900 and 25,400 bp, which join previously known contigs containing levRDEFG, aadK and blt genes near 235 degrees of the Bacillus subtilis chromosome, were sequenced. Among others, two genes, which encode proteins homologous to RNA polymerase sigma-factors, were identified within this region. The gene products designated SigV and SigZ, show the highest homology with sigma-factors encoded by the gene carQ of Myxococcus xanthus and sigX (formerly orfX20) of B. subtilis, correspondingly. All sigma-factors which show statistically significant homology to SigV and SigZ, belong to the ECF (extracytoplasmic functions) subfamily. SigV and SigZ do not have N-terminal sequence which prevents such proteins from binding to DNA without RNA polymerase core enzyme.
authors.affiliation: QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
authors.forename: Alexei
authors.initials: A
authors.lastname: Sorokin
authors.affiliation: QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Bolotin
authors.affiliation: QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Purnelle
authors.affiliation: QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Hilbert
authors.affiliation: QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
authors.forename: Juergen
authors.initials: J
authors.lastname: Lauber
authors.affiliation: QIAGEN GmbH, Max-Volmer-Straße 4, 40724 Hilden, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Düsterhöft
authors.affiliation: Laboratoire de GéAnéAtique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France.
authors.forename: S Dusko
authors.initials: SD
authors.lastname: Ehrlich
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D018387:Codon, Initiator / Q000235:genetics; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D018387:Codon, Initiator; D004269:DNA, Bacterial; C109130:SigV protein, Bacillus subtilis; C109131:SigZ protein, Bacillus subtilis; D012808:Sigma Factor