[Tissue expression of a gene potentially implicated in some diseases with retinal and renal involvement].
We have produced a monoclonal antibody (HA34) that specifically reveals the pigmented epithelium in the eye and the proximal convoluted tubules of the kidney, whatever the developmental stage. The results obtained with the kidney of other mammals suggest that the antigen is human specific. Its molecular weight is approximately 200 kDa. The epitope recognized by HA34 is always present on cell lines grown in vitro. This allowed us to use somatic cell interspecific hybrids to localize the gene implicated in the cytogenetic band 11q13, between microsatellites D11S1777 (AFMa046wa9) and D11S913 (AFM164zf12) in a 9 cM space. This region is involved in forms of retinitis pigmentosa, some of which can also include kidney abnormalities. We propose that this gene is possibly implicated in some of these diseases.
Candelier
J J
JJ
Unité de Recherches sur la Différenciation Cellulaire Intestinale, Inserm U178. Villejuif, France.
Bonneau
D
D
Mennesson
B
B
Coullin
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics; D002880:Chromosomes, Human, Pair 11; D000939:Epitopes; D015870:Gene Expression; D006801:Humans; D007674:Kidney Diseases / Q000150:complications / Q000235:genetics* / Q000276:immunology; D007687:Kidney Tubules, Proximal / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018895:Microsatellite Repeats; D012174:Retinitis Pigmentosa / Q000150:complications / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes