The transactivation domain of adenovirus E1A interacts with the C terminus of human TAF(II)135.
The CR3 activation domain of the human adenovirus E1A protein stimulates transcription by forming protein-protein interactions with DNA sequence-specific binding factors and components of the TFIID complex. Here, we demonstrate that CR3 can complex with the extreme C-terminal 105 amino acids of the human TATA box binding-factor-associated protein, hTAF(II)135. Furthermore, the C-terminal region of hTAF(II)135 can block transcriptional stimulation from an E1A-inducible promoter in vivo. This ability of the C terminus of hTAF(II)135 to bind CR3 and to inhibit E1A-inducible activation is highly specific. These results demonstrate for the first time that a discrete fragment of a mammalian TBP-associated factor which targets a specific activator can impair the stimulation of transcription.
Mazzarelli
J M
JM
Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104, USA.
Mengus
G
G
Davidson
I
I
Ricciardi
R P
RP
D017500:Adenovirus E1A Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000260:Adenoviruses, Human / Q000502:physiology*; D000595:Amino Acid Sequence; D001665:Binding Sites; D005982:Glutathione Transferase; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D035362:Transcription Factor TFIID
D017500:Adenovirus E1A Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C101882:TAF4 protein, human; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D005982:Glutathione Transferase