Genetic control of immune response to a synthetic fimbrial antigen of Actinobacillus actinomycetemcomitans.
The incidence of infection by Actinobacillus actinomycetemcomitans, one of the important pathogens in human periodontal diseases, has been reported to be associated with racial background and genetic factors. We attempted to determine the genetic regulation of immune responses to A. actinomycetemcomitans fimbriae, an attachment factor, using various inbred strains of mice. For this purpose, we synthesized an oligopeptide antigen using the amino acid sequence of the fimbriae and conjugated this antigen to branched lysine polymer resin beads. After immunization with the synthetic A. actinomycetemcomitans fimbrial antigen, serum antibody levels and the delayed-type hypersensitivity (DTH) reaction to the antigen were measured by enzyme-linked immunosorbent assay (ELISA) and footpad swelling responses, respectively. The strains of mice found to be high-IgG responders to the antigen were B10.HTT, B10.RIII, B10.A (5R) and B10.S (9R). These results indicate that mice with E(beta s):E(alpha k), E(beta r):E(alpha r) and E(beta b):E(alpha k) respond strongly to the synthetic peptide. All of the high-IgG responders showed a high DTH response. A cell transfer experiment confirmed that CD4 T cells mediated with DTH response to the synthetic peptide. Thus, the results of this study demonstrate that the immune responses to A. actinomycetemcomitans fimbriae are genetically controlled.
Honma
K
K
Department of Microbiology, Tokyo Dental College, Chiba, Japan.
Ishii
N
N
Kato
T
T
Ishihara
K
K
Okuda
K
K
Okuda
K
K
D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006183:H-2 Antigens / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010510:Periodontal Diseases / Q000382:microbiology*; D014614:Vaccines, Synthetic / Q000276:immunology
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic