title: Cloning of the cDNA for a new member of the immunoglobulin superfamily (ISLR) containing leucine-rich repeat (LRR).
abstract: We isolated cDNAs for a novel protein with a calculated molecular mass of 46 kDa, containing a leucine-rich repeat (LRR) with conserved flanking sequences and a C2-type immunoglobulin (Ig)-like domain. This novel protein was considered to be a new member of the Ig superfamily and was named ISLR (immunoglobulin superfamily containing LRR). These domains are known to be important for protein-protein interaction or cell adhesion, and therefore it is possible that the novel protein ISLR may also interact with other proteins or cells. Northern blot analysis showed the presence of a 2.4-kb transcript in various human tissues including retina, heart, skeletal muscle, prostate, ovary, small intestine, thyroid, adrenal cortex, testis, stomach, and spinal cord as well as fetal lung and fetal kidney. The ISLR gene was mapped on human chromosome 15q23-q24 by fluorescence in situ hybridization.
authors.affiliation: Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nagasawa
authors.forename: R
authors.initials: R
authors.lastname: Kubota
authors.forename: Y
authors.initials: Y
authors.lastname: Imamura
authors.forename: K
authors.initials: K
authors.lastname: Nagamine
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Asakawa
authors.forename: J
authors.initials: J
authors.lastname: Kudoh
authors.forename: S
authors.initials: S
authors.lastname: Minoshima
authors.forename: Y
authors.initials: Y
authors.lastname: Mashima
authors.forename: Y
authors.initials: Y
authors.lastname: Oguchi
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000737:chemistry / Q000235:genetics; D007136:Immunoglobulins / Q000737:chemistry* / Q000235:genetics*; D000090263:Leucine-Rich Repeat Proteins; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D012160:Retina / Q000737:chemistry; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; C108610:ISLR protein, human; D007127:Immunoglobulin Constant Regions; D007136:Immunoglobulins; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins