A two-dimensional, diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique to screen for protease substrates in protein mixtures.
Nestler
H P
HP
Cold Spring Harbor Laboratory, New York 11724, USA. nestler@cshl.org
Doseff
A
A
D000818:Animals; D001345:Autoradiography; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D010450:Endopeptidases / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate; D013379:Substrate Specificity
D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D010450:Endopeptidases