A rapid and accurate strategy for rice contig map construction by combination of fingerprinting and hybridization.
A rapid and accurate strategy for rice contig map construction was described. Rice BAC library with average insert of 120 kb in length was used as building materials in contig mapping. The contigs of varied lengths ranging from 500 kb to several megabases with sufficient redundancy to ensure the accuracy of the joining between individual BACs were formed by fingerprinting. The contigs were then assigned to and ordered along the chromosomes by various molecular markers through their hybridization against the whole rice genomic library. The accuracy of clone overlaps in contig was further confirmed by the existence in contigs of well fit stacks of marker-lodged clones. He contigs thus obtained covered nearly the rice genome.
Hong
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National Center for Gene Research, Chinese Academy of Sciences, Shanghai.
D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D015723:Gene Library; D005822:Genetic Vectors / Q000138:chemical synthesis; D018745:Genome, Plant; D007091:Image Processing, Computer-Assisted; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000235:genetics*; D015183:Restriction Mapping; D012984:Software