title: Proton and phosphorus nuclear magnetic resonance spectroscopy of human brain tumor extracts with automatic data classification: a preliminary study.
abstract: High-resolution one-dimensional proton and phosphorus and two dimensional COSY proton Magnetic Resonance Spectroscopy were used to investigate the lipid and carbohydrate metabolism of human brain tumors. Sixteen meningioma (MG) (benign tumors) and ten glioblastoma (GB) (malignant tumors) samples from brain surgery were treated for dual extraction of lipidic and aqueous phases before NMR processing. A highly significant variation of the 1H metabolite spectral pattern was observed between benign and malignant tumors. Double extraction method combined with both 1H and 31P NMR in vitro analyses provided a large set of biochemical information which may be statistically analyzed to elucidate tumor-specific biochemical pathways and to improve interpretation of in vivo spectra.
authors.affiliation: Laboratoire de Résonance Magnétique en Biologie et Médecine, Faculté de Médicine, Université de Rennes I, France.
authors.forename: L
authors.initials: L
authors.lastname: Nadal
authors.forename: G
authors.initials: G
authors.lastname: Leray
authors.forename: C
authors.initials: C
authors.lastname: Desbarats
authors.forename: F
authors.initials: F
authors.lastname: Darcel
authors.forename: J Y
authors.initials: JY
authors.lastname: Bansard
authors.forename: A
authors.initials: A
authors.lastname: Bondon
authors.forename: J D
authors.initials: JD
authors.lastname: de Certaines
mesh_terms: D001932:Brain Neoplasms / Q000737:chemistry* / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010759:Phosphorus Isotopes; D011522:Protons
chemical_list: D008055:Lipids; D010759:Phosphorus Isotopes; D011522:Protons