Genotoxicity of industrial dyes under the inductive effect of ethanol on monooxygenase system in mice.
The genotoxic effects of triarylmethane (Acid Green 16, C.I.44025) and arylmonoazo (Basic Orange 28, developed by Boruta Pigment Plant, Poland, C.I. undisclosed) dyes, were evaluated in Balb/C mice. Animals were fed for 6 days nutritionally adequate Portagen liquid diet (1 kcal/ml) or isocaloric alcoholic diet containing 5% (w/v) ethanol (36% of total calories) in order to induce the cytochrome P-4502E1 monooxygenase. Dye compounds were administered intraperitoneally 30 h before the test at doses: 90 mg/kg of Acid Green 16 and 70 mg/kg of Basic Orange 28. Bone marrow micronucleus test was used for evaluation of genotoxicity of the dyes. Ethanol caused an increase of the level of cytochrome P-450 by 200% and activities of 7-ethoxycoumarin O-deethylase (ECOD) by 650%, 7-ethoxyresorufin O-deethylase (EROD) by 460% and glutathione (GSH)-S-transferase by 60% in the liver. Both dyes exerted genotoxic effect as inferred from a 3-fold increase of frequency of micronucleated polychromatic erythrocytes in bone marrow, and a further increase (2-fold) was caused by ethanol liquid diet combined with Acid Green 16 treatment. Basic Orange 28 genotoxicity remained unaffected by ethanol. It is concluded that: (1) enhancement of genotoxic effect of Acid Green 16 by ethanol is caused by induction of cytochrome P-4502E1 monooxygenases resulting in an increased bioactivation of the dye; (2) lack of enhancement of the genotoxic effect of Basic Orange 28 by ethanol probably results from the dye- and ethanol-mediated stimulation of GSH-S-transferase, bypassing the cytochrome P-4502E1 bioactivation step.
Wrońska-Nofer
T
T
Department of Toxicological Biochemistry, The Nofer Institute of Occupational Medicine, Lodz, Poland. impx@krysia.uni.lodz.pl
Wiśniewska-Knypl
J
J
Wyszyńska
K
K
Dziubałtowska
E
E
D000818:Animals; D001391:Azo Compounds / Q000378:metabolism / Q000633:toxicity*; D001711:Biotransformation; D001853:Bone Marrow / Q000187:drug effects; D004396:Coloring Agents / Q000378:metabolism / Q000633:toxicity*; D019392:Cytochrome P-450 CYP2E1 / Q000096:biosynthesis / Q000378:metabolism*; D004790:Enzyme Induction; D004912:Erythrocytes / Q000166:cytology; D000431:Ethanol / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood; D008086:Lissamine Green Dyes / Q000378:metabolism / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000201:enzymology; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*
D001391:Azo Compounds; C108241:Basic Orange 28; D004396:Coloring Agents; D008086:Lissamine Green Dyes; D009153:Mutagens; C088063:Acid Green 16; D000431:Ethanol; D007064:L-Iditol 2-Dehydrogenase; D019392:Cytochrome P-450 CYP2E1; D005982:Glutathione Transferase