Enzymatic removal and disinfection of bacterial biofilms.
Model biofilms of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas fluorescens, and Pseudomonas aeruginosa were made on steel and polypropylene substrata. Plaque-resembling biofilms of Streptococcus mutans, Actinomyces viscosus, and Fusobacterium nucleatum were made on saliva-coated hydroxyapatite. The activity of enzymes against bacterial cells in biofilm was measured by fluorescence microscopy and an indirect conductance test in which evolution of carbon dioxide was measured. Glucose oxidase combined with lactoperoxidase was bactericidal against biofilm bacteria but did not remove the biofilm from the substrata. A complex mixture of polysaccharide-hydrolyzing enzymes was able to remove bacterial biofilm from steel and polypropylene substrata but did not have a significant bactericidal activity. Combining oxidoreductases with polysaccharide-hydrolyzing enzymes resulted in bactericidal activity as well as removal of the biofilm.
Johansen
C
C
Novo Nordisk A/S, Novo Alle, Bagsvoerd, Denmark. cjoh@novo.dk
Falholt
P
P
Gram
L
L
D001419:Bacteria / Q000187:drug effects; D001431:Bacteriological Techniques; D018441:Biofilms / Q000187:drug effects; D015169:Colony Count, Microbial; D003263:Contact Lenses, Hydrophilic / Q000009:adverse effects; D004203:Disinfection / Q000379:methods; D017886:Durapatite; D004798:Enzymes / Q000494:pharmacology; D005069:Evaluation Studies as Topic; D005949:Glucose Oxidase / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007784:Lactoperoxidase / Q000494:pharmacology; D008954:Models, Biological; D011126:Polypropylenes; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011551:Pseudomonas fluorescens / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013232:Steel; D013295:Streptococcus mutans / Q000187:drug effects; D013499:Surface Properties
D004798:Enzymes; D011126:Polypropylenes; D013232:Steel; D017886:Durapatite; D005949:Glucose Oxidase; D007784:Lactoperoxidase