title: Modulation of voltage-dependent K+ channel by redox potential in pulmonary and ear arterial smooth muscle cells of the rabbit.
abstract: It has been suggested that hypoxic pulmonary vasoconstriction (HPV) results from the depolarization that is induced by the suppression of K+ current in pulmonary arterial smooth muscle cells (PASMC). We tested the hypothesis that the effect of the cellular redox potential on voltage-sensitive K+ (Kv) current is involved in HPV as a primary sensing mechanism. Kv current in PASMC and ear arterial smooth muscle cells (EASMC) of the rabbit was recorded using the whole-cell patch-clamp technique, and the effect of redox agents [dithiothreitol, DTT and 2,2'-dithio-bis(5-nitropyridine), DTBNP] was tested. Kv current was decreased by DTT, but increased by DTBNP. DTT accelerated the inactivation kinetics, but did not affect steady-state activation and inactivation, whereas DTBNP accelerated activation kinetics. Kv current has a non-inactivating window in the range of from -40 mV to +10 mV. The resting membrane potential measured using the nystatin-perforated-patch method, however, lay between -50 mV and -30 mV and was not depolarized by 5 mM 4-aminopyridine. The membrane-impermeable oxidizing agent DTNB has no effect on Kv current, suggesting that redox modulation sites are intracellular sulphydryl groups. In EASMC, Kv current was decreased by DTT, but increased by DTBNP, indicating that the redox-potential-induced modulation of Kv current in EASMC and in PASMC is the same. It is therefore concluded that Kv current is modulated by the cellular redox potential, but that this modulation is not involved in HPV as a primary sensing mechanism.
authors.affiliation: Department of Physiology and Biophysics, Seoul National University College of Medicine, 28 Yonkeun-Dong, Chongno-Ku, Seoul, 110-799, Korea.
authors.forename: M K
authors.initials: MK
authors.lastname: Park
authors.forename: Y M
authors.initials: YM
authors.lastname: Bae
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: W K
authors.initials: WK
authors.lastname: Ho
authors.forename: Y E
authors.initials: YE
authors.lastname: Earm
mesh_terms: D000818:Animals; D001158:Arteries / Q000502:physiology; D004229:Dithiothreitol / Q000494:pharmacology; D004423:Ear / Q000098:blood supply*; D004553:Electric Conductivity; D004594:Electrophysiology; D005260:Female; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology*; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011651:Pulmonary Artery / Q000166:cytology / Q000502:physiology*; D011725:Pyridines / Q000494:pharmacology; D011817:Rabbits; D019163:Reducing Agents / Q000494:pharmacology
chemical_list: D016877:Oxidants; D015221:Potassium Channels; D011725:Pyridines; D019163:Reducing Agents; C011801:2,2'-dithiobis(5-nitropyridine); D004229:Dithiothreitol