title: Efficient gene transduction by Epstein-Barr-virus-based vectors coupled with cationic liposome and HVJ-liposome.
abstract: We show here a novel non-viral strategy to transduce human cells by using an EBV-based vector system. The EBV-based vectors, the plasmid vectors carrying EBV oriP (origin for plasmid replication) and EBNA (EBV nuclear antigen) 1 gene from EBV genome, were combined with 2 gene delivery systems, i.e., cationic liposome and HVJ-liposome. By both methods, EBV-based vectors could be more efficiently transfected into HeLa cells than non-EBV, conventional plasmid vectors. When human primary fibroblasts were transfected, EBV-based vectors coupled with cationic liposome but HVJ-liposome resulted in successful gene transduction, while human bone marrow cells were transduced with both HVJ-liposome- and cationic liposome-EBV vectors. These results suggest the potential applications of the EBV-based vector system for gene therapy.
authors.affiliation: Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Satoh
authors.forename: M
authors.initials: M
authors.lastname: Osawa
authors.forename: K
authors.initials: K
authors.lastname: Tomiyasu
authors.forename: H
authors.initials: H
authors.lastname: Hirai
authors.forename: C
authors.initials: C
authors.lastname: Shimazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Oda
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
authors.forename: M
authors.initials: M
authors.lastname: Kondo
authors.forename: S
authors.initials: S
authors.lastname: Kinoshita
authors.forename: O
authors.initials: O
authors.lastname: Mazda
authors.forename: J
authors.initials: J
authors.lastname: Imanishi
mesh_terms: D001854:Bone Marrow Cells; D002412:Cations; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008081:Liposomes; D018108:Respirovirus / Q000235:genetics*; D014162:Transfection
chemical_list: D002412:Cations; D008081:Liposomes