Selective block in erythropoietin-induced differentiation of growth factor-independent retrovirally-infected TF-1 cells.
The erythroleukaemic cell line TF-1, infected with either the pBabe neo retrovirus or the retrovirus bearing the human erythropoietin (hEpo) gene, developed three growth factor-independent clones. Erythropoietin (Epo), interleukin-3 (IL-3) and granulocyte-macrophage colony stimulating factor (GM-CSF) accelerated the proliferation of these clones. Autonomous growth of the clones was independent of Epo because it was not altered by Epo anti-sense oligonucleotides, nor was Epo detectable in culture supernatants. Cells from the mutant clones could not be induced by Epo to express glycophorin A and haemoglobin synthesis was markedly reduced. Haemin reversed the block in Epo-induced haemoglobin synthesis. Acquisition of growth factor-independence appears to be linked with the selective loss of differentiation capacity. These cells may provide a useful model for the study of the mechanisms involved in leukaemic transformation.
Percy
M J
MJ
Department of Haematology, Queen's University of Belfast, N. Ireland, U.K.
Lappin
T R
TR
Buckley
O M
OM
McMullin
M F
MF
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D017931:DNA Primers; D004921:Erythropoietin / Q000096:biosynthesis* / Q000494:pharmacology*; D006021:Glycophorins / Q000096:biosynthesis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000494:pharmacology*; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012190:Retroviridae; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D006021:Glycophorins; D006454:Hemoglobins; D007377:Interleukin-3; D016376:Oligonucleotides, Antisense; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor