title: Effects of dexamethasone treatment on IL-1 receptor mRNA levels in vivo.
abstract: Using semiquantitative reverse transcriptasepolymerase chain reaction and Northern analysis, we observed in vivo up-regulation of interleukin-1 (IL-1) RI and IL-1RII mRNA levels in peripheral blood mononuclear cells (PBMCs) and neutrophils (PMNs) from Holstein cattle injected with dexamethasone (0.04 mg/kg). Baseline levels of IL-1RI mRNA were greater than IL-1RII mRNA levels in PBMCs and PMNs before dexamethasone treatment. This is in contrast with the previously reported predominance of IL-1RII in unstimulated human PMNs. IL-1RII mRNA was strongly induced in both bovine PBMCs and PMNs at 24 h and returned to baseline levels by 72 h, after dexamethasone injection. Conversely, the greatest increase in IL-1RI mRNA in PBMCs and PMNs was not detected until 72 h after dexamethasone injection. These data provide evidence for sequential in vivo up-regulation of first IL-1RII mRNA and later IL-1RI mRNA by dexamethasone that is consistent with the anti-inflammatory activity of glucocorticoids.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, USA.
authors.forename: P W
authors.initials: PW
authors.lastname: Yu
authors.forename: L A
authors.initials: LA
authors.lastname: Schuler
authors.forename: M
authors.initials: M
authors.lastname: Kehrli
authors.forename: L
authors.initials: L
authors.lastname: Mattocks
authors.forename: B J
authors.initials: BJ
authors.lastname: Nonnecke
authors.forename: C J
authors.initials: CJ
authors.lastname: Czuprynski
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002417:Cattle; D003907:Dexamethasone / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D017472:Receptors, Interleukin-1 / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D003907:Dexamethasone