Primer/template-independent synthesis of poly d(A-T) by Taq polymerase.
Taq DNA polymerase polymerized dATP and dTTP to poly d(A-T) without requiring added primer/template in the temperature range of 60-70 degrees C. Tth DNA polymerase also catalyzed the reaction, while delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not. The reaction was distinct from the template-nonrequiring terminal deoxynucleotidyl transferase reaction which absolutely required primers.
Hanaki
K
K
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
Odawara
T
T
Muramatsu
T
T
Kuchino
Y
Y
Masuda
M
M
Yamamoto
K
K
Nozaki
C
C
Mizuno
K
K
Yoshikura
H
H
D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D017931:DNA Primers / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D007700:Kinetics; D011067:Poly dA-dT / Q000096:biosynthesis* / Q000737:chemistry; D013379:Substrate Specificity; D019914:Taq Polymerase; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism
D017931:DNA Primers; D003838:Deoxyadenine Nucleotides; D013942:Thymine Nucleotides; D011067:Poly dA-dT; D019914:Taq Polymerase; D004253:DNA Nucleotidylexotransferase; D004259:DNA-Directed DNA Polymerase; C024157:thymidine 5'-triphosphate