title: Synthesis of L-2,4-diamino[4-11C]butyric acid and its use in some in vitro and in vivo tumour models.
abstract: L-2,4-Diamino[4-11C]butyric acid (DAB) was synthesized by an enzyme catalysed carrier added (0.1 micromol KCN) reaction of hydrogen [11C]cyanide with O-acetyl-L-serine followed by reduction. L-[11C]DAB was obtained with a radiochemical purity higher than 96% and with a decay corrected radiochemical yield of 30-40% within a 32 min reaction time. The enantiomeric excess was 98%. The uptake of L-[11C]DAB was investigated in multicellular aggregates of six different cell lines and animal tumour models. L-[11C]DAB is potentially useful for the assessment of pharmacokinetics of L-DAB in vivo for part of its evaluation as an antitumoural agent, although its use for diagnostic purposes seems limited.
authors.affiliation: Subfemtomole Biorecognition Project, Research Development Corporation of Japan, Osaka.Gunnar.Antoni@pet.uu.se
authors.forename: G
authors.initials: G
authors.lastname: Antoni
authors.forename: H
authors.initials: H
authors.lastname: Omura
authors.forename: M
authors.initials: M
authors.lastname: Bergström
authors.forename: Y
authors.initials: Y
authors.lastname: Furuya
authors.forename: R
authors.initials: R
authors.lastname: Moulder
authors.forename: A
authors.initials: A
authors.lastname: Roberto
authors.forename: A
authors.initials: A
authors.lastname: Sundin
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: B
authors.initials: B
authors.lastname: Långström
mesh_terms: D000613:Aminobutyrates / Q000493:pharmacokinetics; D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging*; D051381:Rats; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed; D014407:Tumor Cells, Cultured
chemical_list: D000613:Aminobutyrates; D002250:Carbon Radioisotopes; C005959:2,4-diaminobutyric acid