title: Progress in an ecotoxicological standard protocol with protozoa: results from a pilot ring test with Tetrahymena pyriformis.
abstract: The aim of this project was to develop a standardized multigeneration growth inhibition test protocol to assess the sublethal effects of industrial chemicals and pesticides on protozoans. The inclusion of a test with protozoa would be an ecologically relevant supplement to the existing basic set in aquatic toxicity testing. The different aspects evaluated in the pilot ring study include: two media, two volumes, two end points and two different methods to measure the cell density. Taking into account the aim to develop a simple, sensitive, reproducible and cost effective test, recommendations are made for a standardized protocol.
authors.affiliation: Danish Technological Institute, Taastrup, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Larsen
authors.forename: T W
authors.initials: TW
authors.lastname: Schultz
authors.forename: L
authors.initials: L
authors.lastname: Rasmussen
authors.forename: R
authors.initials: R
authors.lastname: Hooftman
authors.forename: W
authors.initials: W
authors.lastname: Pauli
mesh_terms: D020003:1-Octanol / Q000633:toxicity; D019297:2,4-Dinitrophenol / Q000633:toxicity; D000818:Animals; D000871:Anthelmintics / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D002733:Chlorophenols / Q000633:toxicity; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D017753:Ecosystem; D001556:Hexachlorocyclohexane / Q000633:toxicity; D007306:Insecticides / Q000633:toxicity; D011192:Potassium Dichromate / Q000633:toxicity; D012997:Solvents / Q000633:toxicity; D013769:Tetrahymena pyriformis / Q000187:drug effects* / Q000254:growth & development; D018675:Toxicity Tests / Q000592:standards*; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D000871:Anthelmintics; D002733:Chlorophenols; D003470:Culture Media; D007306:Insecticides; D012997:Solvents; D015262:Xenobiotics; D001556:Hexachlorocyclohexane; D020003:1-Octanol; D019297:2,4-Dinitrophenol; C004762:2,4-dichlorophenol; D011192:Potassium Dichromate