Biovar diversity is reflected by variations of genes encoding urease of Ureaplasma urealyticum.
Five oligonucleotide primers derived from the gene encoding urease of Ureaplasma urealyticum were designed to evaluate the relationship between the urease gene and biovar diversity of this organism. Five combinations of these primers were tested by PCR and the result revealed that there were variations in urease genes among different serovars of U. urealyticum. This result, in agreement with other PCRs based on other functionally unrelated (rRNA and MB antigen) genes, may reflect the phylogenetic relationship among organisms taxonomically classified as U. urealyticum.
Ruifu
Y
Y
Laboratory for Analytical Microbiology, Institute of Microbiology and Epidemiology, Fengtai District, Beijing, P.R. of China.
Minli
Z
Z
Guo
Z
Z
Wang
X
X
D017931:DNA Primers; D005798:Genes, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D016990:Ureaplasma urealyticum / Q000201:enzymology / Q000235:genetics*; D014510:Urease / Q000235:genetics*
D017931:DNA Primers; D014510:Urease