title: Importance of the galE gene on the virulence of Pasteurella multocida.
abstract: The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduced amino acid sequence displayed the highest identity (85%) to the GalE protein of Haemophilus influenzae. However, the gene organization surrounding the galE locus was different from that of H. influenzae. A galE-defective mutant of P. multocida was obtained by replacement of the active galE gene by a copy inactivated in vitro. The resulting galE mutant was highly attenuated as seen in a biological test carried out in a mouse model.
authors.affiliation: Department of Genetics and Microbiology, Universitat Autónoma de Barcelona, Bellaterra, Spain.
authors.forename: A R
authors.initials: AR
authors.lastname: Fernández de Henestrosa
authors.forename: I
authors.initials: I
authors.lastname: Badiola
authors.forename: M
authors.initials: M
authors.lastname: Saco
authors.forename: A M
authors.initials: AM
authors.lastname: Perez de Rozas
authors.forename: S
authors.initials: S
authors.lastname: Campoy
authors.forename: J
authors.initials: J
authors.lastname: Barbé
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D016979:Pasteurella multocida / Q000235:genetics* / Q000472:pathogenicity; D014534:UDPglucose 4-Epimerase / Q000235:genetics*; D014774:Virulence
chemical_list: D014534:UDPglucose 4-Epimerase; C020551:galactose epimerase