Molecular cloning, organization and localization of the gene for the mouse neuropeptide Y-Y5 receptor.
A cDNA clone homologous with the human neuropeptide Y (NPY)-Y5 receptor has been isolated from a mouse brain cDNA library. Analysis of the predicted amino acid sequence indicates that the polypeptide encoded by this cDNA is 89% and 97% identical to the human and rat NPY-Y5 receptors, respectively, but has a repeat sequence of 21-amino acid residues, unlike the human and rat NPY-Y5 receptors, in its N-terminal region. Northern blot analysis indicated that the mouse NPY-Y5 receptor mRNA was highly expressed in the brain, but not in the heart, kidney, spleen, lung, liver, skeletal muscle, and testis. A genomic DNA clone encoding the mouse NPY-Y5 receptor has also been cloned and used as a probe for fluorescence in situ hybridization analysis. The mouse NPY-Y5 receptor gene consists of at least two exons with a 7.0 kbp-long intronic sequence and is localized in the chromosome 8B3-C2 region. Restriction mapping, partial sequencing, and hybridization analysis of the mouse NPY-Y5 and NPY-Y1 receptor genes were used to determine the relationship between the two genes in the chromosome 8B3-C2 region. The mouse NPY-Y5 receptor gene can map about 10 kbp upstream of the transcriptional initiation sites of the NPY-Y1 receptor gene with an opposite orientation.
Nakamura
M
M
Pharmaceutical Basic Research Laboratories (Aobadai), Japan Tobacco Inc., Kanagawa. nakamura@tularik.com
Yokoyama
M
M
Watanabe
H
H
Matsumoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018628:Gene Dosage; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D017476:Receptors, Neuropeptide Y / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D017476:Receptors, Neuropeptide Y; C100568:neuropeptide Y5 receptor