Induction of tolerance in rat cortical neurons: hypoxic preconditioning.
Sublethal ischemia leads to increased tolerance against subsequent prolonged cerebral ischemia in vivo. In the present study we modeled preconditioning mechanisms in a neuronal-enriched culture. Damage was significantly reduced (up to 72%) with 1.5 h of oxygen-glucose deprivation 48-72 h before 3 h oxygen-glucose deprivation. Tolerance was also elicited by Na+-K+-ATPase inhibition. No damage was observed when astroglial or endothelial cells were exposed to hypoxia for 3 and 6 h, respectively. We conclude that hypoxic preconditioning is a robust neuronal phenomenon in vitro with a similar temporal pattern and selective cellular vulnerability as the ischemic tolerance phenomenon shown in vivo.
Bruer
U
U
Department of Neurology, Charité Hospital, Humboldt University, Berlin, Germany.
Weih
M K
MK
Isaev
N K
NK
Meisel
A
A
Ruscher
K
K
Bergk
A
A
Trendelenburg
G
G
Wiegand
F
F
Victorov
I V
IV
Dirnagl
U
U
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D004587:Electrophoresis, Agar Gel; D005260:Female; D005947:Glucose / Q000378:metabolism; D019194:Ischemic Preconditioning; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D010042:Ouabain; D007770:L-Lactate Dehydrogenase; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose