High-performance liquid chromatography of methamphetamine and its related compounds in human urine following derivatization with fluorescein isothiocyanate.
A high-performance liquid chromatographic method with fluorescence detection for the determination of methamphetamine and its related compounds is reported. Methamphetamine, amphetamine, norephedrine, p-hydroxymethamphetamine and 1-phenylethylamine as an internal standard were extracted from human urine, derivatized with fluorescein-4-isothiocyanate, and then separated on a reversed-phase column within 36 min. The fluorescence intensity of the effluent was monitored at excitation and emission wavelengths of 496 and 518 nm, respectively. Calibration curves were confirmed to be linear up to at least 100 pmol on the column with a correlation coefficient (r) of 0.994-0.999 for the target compounds. The detection limits (S/N=3) were 55-105 fmol per 20-microl injection. The method was successfully applied to urine samples taken from methamphetamine addicts.
Al-Dirbashi
O
O
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Kuroda
N
N
Akiyama
S
S
Nakashima
K
K
D000661:Amphetamine / Q000652:urine; D000697:Central Nervous System Stimulants / Q000378:metabolism / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D008694:Methamphetamine / Q000378:metabolism / Q000652:urine*; D010665:Phenylpropanolamine / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D019966:Substance-Related Disorders / Q000378:metabolism / Q000652:urine*; D010137:p-Hydroxynorephedrine / Q000652:urine
D000697:Central Nervous System Stimulants; D005456:Fluorescent Dyes; D010665:Phenylpropanolamine; D008694:Methamphetamine; D010137:p-Hydroxynorephedrine; D000661:Amphetamine; D016650:Fluorescein-5-isothiocyanate