Method for the analysis of perhexiline and its hydroxy metabolite in plasma using high-performance liquid chromatography with precolumn derivatization.
A high-performance liquid chromatographic method for the analysis of perhexiline and its monohydroxy metabolite in plasma has been developed. After a simple extraction procedure, the analytes are derivatized over a 30-min period with trans-4-nitrocinnamoyl chloride. The derivatized products are monitored at 340 nm following separation on a 5-micron phenyl reversed-phase column under isocratic conditions. The limits of detection for perhexiline and its hydroxy metabolite are 0.03 and 0.02 mg/l, respectively. The between-day and within-day assay coefficients of variation for perhexiline and its hydroxy metabolite at concentrations of 0.2 and 1.0 mg/I were less than 10%. The method has proved robust and suitable for the routine monitoring of perhexiline and hydroxyperhexiline.
Grgurinovich
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Department of Clinical Pharmacology, Flinders Medical Centre, Adelaide, Australia.
D002317:Cardiovascular Agents / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002940:Circadian Rhythm; D004347:Drug Interactions; D005021:Ethylamines / Q000737:chemistry; D006801:Humans; D006878:Hydroxides / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D016014:Linear Models; D010480:Perhexiline / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results
D002317:Cardiovascular Agents; D005021:Ethylamines; D006878:Hydroxides; D010480:Perhexiline; C041564:ethylamine