Initiation and bidirectional propagation of chromatin assembly from a target site for nucleotide excision repair.
To restore full genomic integrity in a eukaryotic cell, DNA repair processes have to be coordinated with the resetting of nucleosomal organization. We have established a cell-free system using Drosophila embryo extracts to investigate the mechanism linking de novo nucleosome formation to nucleotide excision repair (NER). Closed-circular DNA containing a uniquely placed cisplatin-DNA adduct was used to follow chromatin assembly specifically from a site of NER. Nucleosome formation was initiated from a target site for NER. The assembly of nucleosomes propagated bidirectionally, creating a regular nucleosomal array extending beyond the initiation site. Furthermore, this chromatin assembly was still effective when the repair synthesis step in the NER process was inhibited.
Gaillard
Pierre-Henri L
PHL
Institut Curie/Research section UMR 144 du CNRS, 26 rue d'Ulm 75231 Paris cedex 05, France.
Moggs
J G
JG
Roche
D M
DM
Quivy
J P
JP
Becker
P B
PB
Wood
R D
RD
Almouzni
G
G
D000818:Animals; D002474:Cell-Free System; D002843:Chromatin / Q000378:metabolism*; D002945:Cisplatin / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D018736:DNA Adducts / Q000378:metabolism; D004260:DNA Repair; D004270:DNA, Circular / Q000378:metabolism; D004330:Drosophila; D004625:Embryo, Nonmammalian; D006801:Humans; D008957:Models, Genetic; D014981:Xenopus
D002843:Chromatin; D018736:DNA Adducts; D004270:DNA, Circular; D004247:DNA; D002945:Cisplatin