title: The structure of alfalfa mosaic virus capsid protein assembled as a T=1 icosahedral particle at 4.0-A resolution.
abstract: K. Fukuyama, S. S. Abdel-Meguid, J. E. Johnson, and M. G. Rossmann (J. Mol. Biol. 167:873-984, 1983) reported the structure of alfalfa mosaic virus assembled from the capsid protein as a T=1 icosahedral empty particle at 4.5-A resolution. The information contained in the structure included the particle size, protein shell thickness, presence of wide holes at the icosahedral fivefold axes, and a proposal that the capsid protein adopts a beta-barrel structure. In the present work, the X-ray diffraction data of Fukuyama et al. as well as the data subsequently collected by I. Fita, Y. Hata, and M. G. Rossmann (unpublished) were reprocessed to 4.0-A resolution, and the structure was solved by molecular replacement. The current structure allowed the tracing of the polypeptide chain of the capsid protein confirming the beta-sandwich fold and provides information on intersubunit interactions in the particle. However, it was not possible to definitively assign the amino acid sequence to the side chain density at 4-A resolution. The particle structure was also determined by cryoelectron microscopy and image reconstruction methods and found to be in excellent agreement with the X-ray model.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: V S
authors.initials: VS
authors.lastname: Reddy
authors.forename: V
authors.initials: V
authors.lastname: Yusibov
authors.forename: P R
authors.initials: PR
authors.lastname: Chipman
authors.forename: Y
authors.initials: Y
authors.lastname: Hata
authors.forename: I
authors.initials: I
authors.lastname: Fita
authors.forename: K
authors.initials: K
authors.lastname: Fukuyama
authors.forename: M G
authors.initials: MG
authors.lastname: Rossmann
authors.forename: L S
authors.initials: LS
authors.lastname: Loesch-Fries
authors.forename: T S
authors.initials: TS
authors.lastname: Baker
authors.forename: J E
authors.initials: JE
authors.lastname: Johnson
mesh_terms: D017794:Alfalfa mosaic virus / Q000737:chemistry / Q000648:ultrastructure*; D002213:Capsid / Q000737:chemistry* / Q000648:ultrastructure*; D005615:Freezing; D000455:Medicago sativa / Q000821:virology; D008854:Microscopy, Electron; D008958:Models, Molecular; D008961:Models, Structural; D008962:Models, Theoretical; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction