The three subtypes of atrial natriuretic peptide (ANP) receptors are expressed in the rat adrenal gland.
Atrial natriuretic peptide (ANP) actions are mediated by highly selective and specific receptors. Three subtypes have been characterized and cloned: ANP receptor-A (or GC-A), -B (or GC-B) and -C (the so-called clearance receptor). In rat adrenal gland, the mRNA for each subtype was detected using 35S-dUTP or digoxigenin-11-dUTP specific labeled probes, and in situ hybridization at light and electron microscopic levels respectively. The three subtypes were expressed the most abundantly in the zona glomerulosa. The amount of GC-A mRNA expression, quantified using macro-autoradiography and densitometry, was higher than the amounts of GC-B mRNA and ANPR-C mRNA both in zona glomerulosa and medullary of adrenal gland. At electron microscopic level, the three subtypes of ANPR were revealed in glomerulosa cells. A noticeable signal was also present in the medullary area, especially for GC-A mRNA, in adrenaline-containing chromaffin cells. No signal was detected in noradrenaline-containing chromaffin cells. The subcellular localization of the three mRNAs is similar: in the cytoplasmic matrix and in the euchromatin of the nucleus in each cell of glomerulosa, and in the same compartments of the adrenaline-containing chromaffin cells. These data indicate that the adrenal gland is an important target tissue for ANP action both in glomerulosa cells and adrenaline-containing chromaffin cells. The mRNA expression levels were different for each ANPR subtype.
Grandclément
B
B
CNRS-URA 1459, Institut Pasteur de Lyon, France.
Ronsin
B
B
Morel
G
G
D000313:Adrenal Medulla / Q000737:chemistry / Q000648:ultrastructure; D000818:Animals; D001345:Autoradiography; D015342:DNA Probes; D005260:Female; D015870:Gene Expression / Q000502:physiology; D017403:In Situ Hybridization; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics*; D013462:Sulfur Radioisotopes; D015384:Zona Glomerulosa / Q000737:chemistry* / Q000648:ultrastructure
D015342:DNA Probes; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D017461:Receptors, Atrial Natriuretic Factor