title: Lipid and fatty acid compositions of a novel docosahexaenoic acid-producing marine bacterium.
abstract: An unidentified bacterial strain, SCRC-21406, isolated from the intestine of a marine fish, Glossanodon semifasciatus, produced docosahexaenoic acid at 23% (mol/mol) [= 28% (w/w)] of total fatty acids in a medium containing 0.5% (wt/vol) peptone and 0.1% (wt/vol) yeast extract at 12 degrees C under atmospheric pressure. The cell yield was 0.43 g/L. The major lipids of the strain were phosphatidylethanolamine and phophatidylglycerol. Docosahexaenoic acid was localized at the sn-2 positions of both phospholipids. The amounts of polyunsaturated fatty acids other than docosahexaenoic acid were extremely small [< 3% (mol/mol)]. Monounsaturated fatty acids of the cis-7, cis-9 and cis-11 types were detected.
authors.affiliation: Sagami Chemical Research Center, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: C
authors.initials: C
authors.lastname: Ishikawa
authors.forename: I
authors.initials: I
authors.lastname: Ohtsuka
authors.forename: M
authors.initials: M
authors.lastname: Kamata
authors.forename: M
authors.initials: M
authors.lastname: Tomita
authors.forename: K
authors.initials: K
authors.lastname: Yazawa
authors.forename: H
authors.initials: H
authors.lastname: Muramatsu
mesh_terms: D000818:Animals; D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005399:Fishes / Q000382:microbiology; D007564:Japan; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D008968:Molecular Conformation; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry; D013053:Spectrophotometry
chemical_list: D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D004281:Docosahexaenoic Acids