Cytoplasmic tyrosyl-tRNA synthetase rescues the defect in mitochondrial genome maintenance caused by the nuclear mutation mgm104-1 in the yeast Saccharomyces cerevisiae.
The yeast nuclear mutation mgm104-1, which leads to slow growth on glucose medium and temperature-sensitive (ts) loss of mitochondrial DNA (mtDNA), has been identified by screening a collection of temperature-sensitive mutants on glycerol medium. A nuclear gene was isolated from a genomic DNA library by complementation of the mgm104-1 allele and was found to be identical to TTS1, which encodes the cytoplasmic tyrosyl-tRNA synthetase required for cytoplasmic protein synthesis. A gene disruption in a diploid strain demonstrated that the TTS1 gene is essential for cell viability. The lack of mutations in TTS1 in the mgm104-1 mutant indicates that TTS1 and MGM104 are different genes. The ability to rescue the mgm104-1 phenotype with a single additional copy of TTS1 suggests that TTS1 has an additional function that is directly or indirectly involved in the maintenance of the mitochondrial genome.
Guan
M X
MX
Molecular and Population Genetics Group, Research School of Biological Sciences, The Australian National University, Canberra. guan@seqaxp.bio.caltech.edu
D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000201:enzymology; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D013696:Temperature; D014448:Tyrosine-tRNA Ligase / Q000378:metabolism*
D017931:DNA Primers; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D014448:Tyrosine-tRNA Ligase