title: Inhibition of microbial growth in chub-packed ground beef by refrigeration (2 degrees C) and medium-dose (2.2 to 2.4 kGy) irradiation.
abstract: Two studies were conducted to monitor microbial growth in chub-packed ground beef. In the first study, the effect of storage temperatures (2 and 7 degrees C) on chubs was examined for up to 18 days. Storage of chubs at 2 degrees C slowed microbial growth such that total counts exceeded 7.5 log10 cfu/g within 10 days compared to within 4 days for chubs stored at 7 degrees C. Microbial counts > or = 7.5 log10 cfu/g were associated with off-odors and samples were considered spoiled. The predominant microbes were homofermentative lactococci, although a gas-producing bacterium, Hafnia alvei, was prevalent in one of three trials. In the second study, medium dose (2.2 to 2.4 kGy) X-ray irradiation extended the shelf-life of chub-packed ground beef at 2 degrees C by approximately 14 days. Microbial counts on irradiated ground beef did not exceed 7.5 log10 cfu/g during 34 days of storage, while this level was reached in non-irradiated chubs by day 13. The predominant microbes on both non-irradiated and irradiated chubs were homofermentative lactococci; H. alvei was not isolated in the second study. These data indicated that refrigerated (2 degrees C) storage significantly delayed microbial spoilage in chub-packed ground beef, and that spoilage was further delayed when refrigerated (2 degrees C) ground beef chubs were exposed to medium dose irradiation.
authors.affiliation: University of Wisconsin, Dept. of Food Science, Madison 53706, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Gamage
authors.forename: N G
authors.initials: NG
authors.lastname: Faith
authors.forename: J B
authors.initials: JB
authors.lastname: Luchansky
authors.forename: D R
authors.initials: DR
authors.lastname: Buege
authors.forename: S C
authors.initials: SC
authors.lastname: Ingham
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development*; D002417:Cattle; D005514:Food Irradiation; D006863:Hydrogen-Ion Concentration; D008460:Meat / Q000382:microbiology*; D012034:Refrigeration; D013696:Temperature