Mutations in the carboxy-terminal part of IS30 transposase affect the formation and dissolution of (IS30)2 dimer.
The transposase of IS30 catalyses different transpositional rearrangements via the dimer (IS30)2 intermediate structure. Mutation analysis provides evidence that the C-terminal part of IS30 transposase is required for the formation and dissolution of (IS30)2 dimer. C-terminal mutants are also defective in transpositional fusion; however, this deficiency can be 'suppressed' by addition of the final product of site-specific dimerisation, the core (IS30)2 intermediate structure. The transposase part studied shows significant homologies in three highly conserved regions to proteins of IS30-related mobile elements.
Olasz
F
F
Abteilung Mikrobiologie, Biozentrum der Universität Basel, Switzerland. olasz@abc.hu
Farkas
T
T
Stalder
R
R
Arber
W
W
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D016384:Consensus Sequence; D004252:DNA Mutational Analysis; D004254:DNA Nucleotidyltransferases / Q000737:chemistry* / Q000378:metabolism*; D004251:DNA Transposable Elements; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D019281:Dimerization; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013698:Templates, Genetic; D019895:Transposases
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D004254:DNA Nucleotidyltransferases; D019895:Transposases; C025580:endodeoxyribonuclease XBAI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific