[New quantitative method for determining p53 protein in bladder carcinomas].
OBJECTIVE
To continue a study protocol on the molecular biology of bladder tumors, analyze protein p53 expression using a new quantitative analytical method and the biological implications of the changes in p53 expression.


METHODS
From January, 1993 to January, 1995, 74 patients were studied. These patients were divided into two groups: the first group comprised 14 controls of urothelial tissue and the second comprised 60 cases of transitional cell carcinoma of the bladder. A quantitative method of immunoluminescence (LIA-mat p53 method) was utilized to analyze p53 expression.


RESULTS
Tissue oncoprotein p53 was higher in patients with bladder carcinoma than in healthy urothelial tissue. Higher values of protein p53 was found in infiltrating and undifferentiated tumors and in those patients who died than in those who are alive.


CONCLUSIONS
Protein p53 determination using this new quantitative method permits identification of subgroups of patients with tumors that have a more aggressive biological behaviour.
Moreno Sierra
J
J
I Cátedra de Urología, Hospital Universitario San Carlos., Madrid, España.
Maestro de las Casas
M L
ML
Ortega Heredia
M D
MD
Chicharro Almarza
J
J
Blanco Jiménez
E
E
Silmi Moyano
A
A
Resel Estévez
L
L
D000911:Antibodies, Monoclonal / Q000276:immunology; D002295:Carcinoma, Transitional Cell / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D002454:Cell Differentiation; D003600:Cytosol / Q000737:chemistry; D005069:Evaluation Studies as Topic; D005260:Female; D016158:Genes, p53; D006801:Humans; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives; D008297:Male; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis*; D011379:Prognosis; D011446:Prospective Studies; D016019:Survival Analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*; D001749:Urinary Bladder Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D019459:Urothelium / Q000737:chemistry
D000911:Antibodies, Monoclonal; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53; C032487:isoluminol; D008165:Luminol