Histamine release from human bronchoalveolar lavage mast cells by neurokinin A and bradykinin.
OBJECTIVE
This study examined whether bradykinin and neurokinin A activate human pulmonary mast cells retrieved by bronchoalveolar lavage (BAL).


METHODS
BAL samples were obtained at routine bronchoscopy from 14 unpreselected patients.


METHODS
Histamine release experiments were performed using substance P, neurokinin A, bradykinin (peptides 25 and 50 microM), compound 48/80 (0.75-10 micrograms/ml) and A23187 (1 microM). Statistical analyses were performed using the paired Student's t-test and Pearson's linear correlation coefficient.


RESULTS
Compound 48/80 induced release was significantly lower than that induced by the other secretagogues (p < 0.05). Neurokinin A and bradykinin induced release correlated significantly with substance P induced release (p < 0.01), suggesting similar mechanisms of action. No correlations were observed between neurokinin A or bradykinin-induced release and the non-peptide stimuli studied.


CONCLUSIONS
The mechanism of neurokinin A- and bradykinin-induced bronchoconstriction is not yet clear but our data suggest an indirect effect mediated by mast cell degranulation.
Cross
L J
LJ
Department of Clinical Biochemistry, Queen's University of Belfast, UK.
Heaney
L G
LG
Ennis
M
M
D001920:Bradykinin / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D000001:Calcimycin / Q000494:pharmacology; D005260:Female; D006636:Histamine Release; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology*; D008875:Middle Aged; D015288:Neurokinin A / Q000494:pharmacology*; D013373:Substance P / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D007476:Ionophores; D013373:Substance P; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D015288:Neurokinin A; D001920:Bradykinin