Ultrastructural demonstration of the NADPH-diaphorase activity in peritubular myoid cells and fibroblasts of the lamina propria of the mouse seminiferous tubules.
The NOS-related NADPH-diaphorase activity was studied by transmission electron microscopy in the peritubular myoid cells and fibroblasts of normal mouse testis. The reaction product was observed on the membranes of the endoplasmic reticulum, on the Golgi apparatus and nuclear envelope. The peritubular myoid cells and fibroblasts showed similar ultracytochemical features; the intensity of the enzymatic reaction was suggestive of an important role of the NOS/cGMP enzymatic system in these cells. Some hypotheses on the role of NO in the peritubular myoid cells and fibroblasts are proposed.
Ruffoli
R
R
Istituto di Anatomia Umana Normale, Facoltà di Medicina e Chirurgia dell'Università degli Studi di Pisa, Italy.
Menchini Fabris
F
F
Giambelluca
M A
MA
Giannessi
F
F
D000818:Animals; D003238:Connective Tissue / Q000201:enzymology* / Q000648:ultrastructure; D005347:Fibroblasts / Q000201:enzymology* / Q000648:ultrastructure; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D009252:NADPH Dehydrogenase / Q000378:metabolism* / Q000648:ultrastructure; D019001:Nitric Oxide Synthase / Q000378:metabolism / Q000648:ultrastructure; D012671:Seminiferous Tubules / Q000201:enzymology* / Q000648:ultrastructure; D013379:Substrate Specificity
D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase