A CXCR4/CD4 pseudotype rhabdovirus that selectively infects HIV-1 envelope protein-expressing cells.
We show that a cellular virus receptor functions in the envelope of a virus, allowing selective infection of cells displaying the receptor ligand. A G-deficient rabies virus (RV) pseudotyped with CD4- and CXCR4-derived proteins selectively infected cells expressing HIV-1 envelope protein. Envelope protein or CD4 antibodies blocked virus entry. Pseudotype virus formation was most efficient with chimeric receptor proteins possessing the cytoplasmic tail of the RV G spike protein (CXCR4-RV and CD4-RV). While CXCR4-RV was incorporated when expressed alone, CD4-RV incorporation required CXCR4-RV as a carrier protein, indicating a mechanism by which oligomeric surface proteins are sorted into the RV envelope. Viral vectors bearing virus receptors in their envelope may be useful reagents for targeting virus-infected cells in vivo.
Mebatsion
T
T
Department of Clinical Virology, Federal Research Center for Virus Diseases of Animals, Tübingen, Germany.
Finke
S
S
Weiland
F
F
Conzelmann
K K
KK
D000595:Amino Acid Sequence; D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000378:metabolism; D015658:HIV Infections / Q000628:therapy / Q000821:virology*; D015497:HIV-1 / Q000254:growth & development / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000166:cytology; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D019718:Receptors, CXCR4; D015728:Receptors, HIV / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D012209:Rhabdoviridae / Q000737:chemistry / Q000254:growth & development / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000378:metabolism*; D017735:Virus Latency / Q000502:physiology; D014779:Virus Replication / Q000502:physiology
D015704:CD4 Antigens; D002352:Carrier Proteins; D008565:Membrane Proteins; D019718:Receptors, CXCR4; D015728:Receptors, HIV; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D019204:GTP-Binding Proteins