title: Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures.
abstract: A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells; a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.
authors.affiliation: Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
authors.forename: L K
authors.initials: LK
authors.lastname: Poulsen
authors.forename: H M
authors.initials: HM
authors.lastname: Dalton
authors.forename: M L
authors.initials: ML
authors.lastname: Angles
authors.forename: K C
authors.initials: KC
authors.lastname: Marshall
authors.forename: S
authors.initials: S
authors.lastname: Molin
authors.forename: A E
authors.initials: AE
authors.lastname: Goodman
mesh_terms: D001419:Bacteria / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D017404:In Situ Hybridization, Fluorescence; D009016:Moraxella / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D015345:Oligonucleotide Probes; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D015345:Oligonucleotide Probes; D001616:beta-Galactosidase