title: Mutations in the carboxy-terminal part of IS30 transposase affect the formation and dissolution of (IS30)2 dimer.
abstract: The transposase of IS30 catalyses different transpositional rearrangements via the dimer (IS30)2 intermediate structure. Mutation analysis provides evidence that the C-terminal part of IS30 transposase is required for the formation and dissolution of (IS30)2 dimer. C-terminal mutants are also defective in transpositional fusion; however, this deficiency can be 'suppressed' by addition of the final product of site-specific dimerisation, the core (IS30)2 intermediate structure. The transposase part studied shows significant homologies in three highly conserved regions to proteins of IS30-related mobile elements.
authors.affiliation: Abteilung Mikrobiologie, Biozentrum der Universität Basel, Switzerland. olasz@abc.hu
authors.forename: F
authors.initials: F
authors.lastname: Olasz
authors.forename: T
authors.initials: T
authors.lastname: Farkas
authors.forename: R
authors.initials: R
authors.lastname: Stalder
authors.forename: W
authors.initials: W
authors.lastname: Arber
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D016384:Consensus Sequence; D004252:DNA Mutational Analysis; D004254:DNA Nucleotidyltransferases / Q000737:chemistry* / Q000378:metabolism*; D004251:DNA Transposable Elements; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D019281:Dimerization; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013698:Templates, Genetic; D019895:Transposases
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D004254:DNA Nucleotidyltransferases; D019895:Transposases; C025580:endodeoxyribonuclease XBAI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific