Inhibitory monoclonal antibodies to human cytochrome P450 2D6.
Two monoclonal antibodies (MAbs) have been isolated that bind to human P450 2D6 and inhibit 2D6 catalyzed bufuralol 1-hydroxylation by 90%. One but not both of the MAbs immunoblotted 2D6. The MAbs were highly specific to 2D6 and did not cross-react with other P450s. Inhibitory monoclonal antibodies will be useful for determining the contribution of 2D6 to the metabolism of a wide variety of 2D6 and other P450 substrates in human tissues containing multiple P450s.
Krausz
K W
KW
Laboratory of Molecular Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, U.S.A.
Yang
T J
TJ
Gonzalez
F J
FJ
Shou
M
M
Gelboin
H V
HV
D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D019389:Cytochrome P-450 CYP2D6 / Q000096:biosynthesis / Q000276:immunology; D065690:Cytochrome P-450 CYP2D6 Inhibitors; D004983:Ethanolamines / Q000378:metabolism; D006801:Humans; D006900:Hydroxylation; D010616:Phenanthrenes / Q000378:metabolism
D000911:Antibodies, Monoclonal; D065690:Cytochrome P-450 CYP2D6 Inhibitors; D004983:Ethanolamines; D010616:Phenanthrenes; C031181:phenanthrene; C010831:bufuralol; D019389:Cytochrome P-450 CYP2D6