Ammonium acetate protocol for the preparation of plasmid DNA suitable for mammalian cell transfections.
Saporito-Irwin
S M
SM
Washington University School of Medicine, St. Louis, MO 63110, USA.
Geist
R T
RT
Gutmann
D H
DH
D000085:Acetates; D000818:Animals; D000838:Anions; D019556:COS Cells; D002498:Centrifugation; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D025581:Neurofibromin 2; D010446:Peptide Fragments / Q000032:analysis; D010957:Plasmids; D014162:Transfection; D001616:beta-Galactosidase / Q000032:analysis
D000085:Acetates; D000838:Anions; D004396:Coloring Agents; D008565:Membrane Proteins; D025581:Neurofibromin 2; D010446:Peptide Fragments; D004247:DNA; D001616:beta-Galactosidase; D004996:Ethidium; C018824:ammonium acetate