title: Changes in cortical EEG and cholinergic function in response to NGF in rats with nucleus basalis lesions.
abstract: We examined whether recovery of cholinergic function in response to nerve growth factor (NGF) results in restoration of electrocortical activity. Rats received unilateral lesions of the nucleus basalis and were infused intracerebroventricularly (i.c.v.) over 3 weeks with NGF or vehicle. Cortical electrical activity was assessed at postoperative days 4, 7, 14, and 21 from 8 epidural electrodes. On day 21, choline acetyl transferase (ChAT) activity was measured in cortical tissue underlying each electrode site. Lesions resulted in increases in slow-wave (delta) power and decreases in high-frequency (beta 2) power in the lesioned, as well as the non-lesioned hemisphere. Changes correlated topographically and in magnitude with losses of ChAT activity and suggested that regional electrocortical function was affected by cholinergic activity originating in the ipsilateral, as well as the contralateral hemisphere. NGF attenuated changes in cholinergic and electrocortical function bilaterally, though in the lesioned hemisphere, function did not return to control levels. Likewise, intact animals receiving NGF showed increases in beta 2-power, as well as modest increases in ChAT activity. Changes in brain electrical activity in response to NGF occurred within 4-7 days without significant changes during the 2 weeks thereafter. Our results suggest that outcomes of future animal and human trials-using unilateral i.c.v. infusions of NGF need to consider the reciprocal influences of hemispheric cholinergic function, as well as possible effects of NGF on intact brain.
authors.affiliation: Department of Psychiatry, UCLA School of Medicine, USA. dholschneider@qeeg.npi.ucla.edu
authors.forename: D P
authors.initials: DP
authors.lastname: Holschneider
authors.forename: A F
authors.initials: AF
authors.lastname: Leuchter
authors.forename: N Y
authors.initials: NY
authors.lastname: Walton
authors.forename: O U
authors.initials: OU
authors.lastname: Scremin
authors.forename: D M
authors.initials: DM
authors.lastname: Treiman
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects*; D002795:Choline O-Acetyltransferase / Q000187:drug effects*; D004569:Electroencephalography / Q000187:drug effects*; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013377:Substantia Innominata / Q000502:physiology*
chemical_list: D009414:Nerve Growth Factors; D002795:Choline O-Acetyltransferase