title: Expression of globo-series gangliosides in human renal cell carcinoma.
abstract: Gangliosides have been shown to be involved in development, differentiation, oncogenesis, and cancer progression. We investigated immunohistochemical expression of globo-series gangliosides in human renal cell carcinoma (RCC) and whether their expression is related to the clinical course. The expression of globo-series gangliosides was evaluated in fresh-frozen sections of 55 primary renal tumors and 8 metastatic deposits using monoclonal antibodies RM1 and RM2, which define monosialosyl and disialosyl galactosylgloboside, respectively. The immunoreactivity of primary tumors to RM1 and/or RM2 was correlated with the clinicopathological data. Cumulative incidence of metastasis detected at initial diagnosis and during the follow-up period was significantly higher in the cases whose primary tumors were RM1/RM2-positive (RM1 and/or RM2-positive) than in the RM1/ RM2-negative (neither RM1 nor RM2-positive) cases (P < 0.05). During the follow-up period, metastasis developed in none of the RM1/RM2-negative cases which had not shown metastasis at initial diagnosis. High nuclear grade was observed only in the RM1/RM2-positive cases. The RM1/RM2-positive rate of the metastatic deposits was higher than that of the primary tumors. Furthermore, a metastatic deposit obtained from one of the cases whose primary tumors were equivocal for RM1/ RM2 was extensively stained by RM1 and RM2. These results indicate that globo-series gangliosides may be one of the biochemical indicators related to the metastatic potential of human RCC.
authors.affiliation: Department of Urology, Tohoku University School of Medicine, Sendai.
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Orikasa
authors.forename: M
authors.initials: M
authors.lastname: Satoh
authors.forename: C
authors.initials: C
authors.lastname: Ohyama
authors.forename: A
authors.initials: A
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D002240:Carbohydrate Sequence; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D005260:Female; D005732:Gangliosides / Q000096:biosynthesis*; D005915:Globosides / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis
chemical_list: D000911:Antibodies, Monoclonal; D005732:Gangliosides; D005915:Globosides; C086047:disialosyl galactosyl globoside