[Comparative analysis of expression of genes for 2',5'-oligoadenylate synthetase and mRNA for dsRNA-protein kinase in human fibroblasts infected with the Sindbis alphavirus and induced by alpha-interferon].
Expression of 2,5-OAS and ds-protein kinase (ds-PK) in human fibroblast culture following treatment with alpha-IF in a dose of 1000 U or challenge with Karelian fever virus (KFV) (5 PFU/cell, 4 h.p.i.) was compared. A highly sensitive and rapid method, coupled RT-PCR, was used to detect mRNA transcription levels. Alpha-IF had an expressed stimulating effect on 2,5-OAS mRNA level, whereas the virus markedly inhibited the synthesis of ds-PK mRNA.
Sokolova
T M
TM
Bobrova
O V
OV
Lebedev
A Iu
AIu
Bystrov
N S
NS
Uryvaev
L V
LV
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000821:virology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012845:Sindbis Virus / Q000502:physiology*; D014158:Transcription, Genetic; D019892:eIF-2 Kinase
D016898:Interferon-alpha; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; D019892:eIF-2 Kinase; D015088:2',5'-Oligoadenylate Synthetase