title: Rapid colorectal adenoma formation initiated by conditional targeting of the Apc gene.
abstract: Familial adenomatous polyposis coli (FAP) is a disease characterized by the development of multiple colorectal adenomas, and affected individuals carry germline mutations in the APC gene. With the use of a conditional gene targeting system, a mouse model of FAP was created that circumvents the embryonic lethality of Apc deficiency and directs Apc inactivation specifically to the colorectal epithelium. loxP sites were inserted into the introns around Apc exon 14, and the resultant mutant allele (Apc580S) was introduced into the mouse germline. Mice homozygous for Apc580S were normal; however, upon infection of the colorectal region with an adenovirus encoding the Cre recombinase, the mice developed adenomas within 4 weeks. The adenomas showed deletion of Apc exon 14, indicating that the loss of Apc function was caused by Cre-loxP-mediated recombination.
authors.affiliation: Department of Cell Biology, Cancer Institute, Toshima-ku, Tokyo 170, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shibata
authors.forename: K
authors.initials: K
authors.lastname: Toyama
authors.forename: H
authors.initials: H
authors.lastname: Shioya
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Hirota
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: H
authors.initials: H
authors.lastname: Takano
authors.forename: T
authors.initials: T
authors.lastname: Akiyama
authors.forename: K
authors.initials: K
authors.lastname: Toyoshima
authors.forename: R
authors.initials: R
authors.lastname: Kanamaru
authors.forename: Y
authors.initials: Y
authors.lastname: Kanegae
authors.forename: I
authors.initials: I
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Shiba
authors.forename: T
authors.initials: T
authors.lastname: Noda
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D025601:Adenomatous Polyposis Coli Protein; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D003106:Colon / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D004195:Disease Models, Animal; D005091:Exons; D005260:Female; D016368:Frameshift Mutation; D017353:Gene Deletion; D018390:Gene Targeting; D017491:Genes, APC; D005822:Genetic Vectors; D018095:Germ-Line Mutation; D006720:Homozygote; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D007438:Introns; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic; D014764:Viral Proteins
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D003598:Cytoskeletal Proteins; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases