Spiking Survanta with synthetic surfactant peptides improves oxygenation in surfactant-deficient rats.
The hypothesis that the in vivo function of Survanta (Beractant) can be improved by supplementation with synthetic surfactant peptides B and C was tested in a surfactant-deficient rat model. Full length surfactant protein-B (SP-B1-78) (B) and palmitoylated surfactant protein-C (SP-C1-35) (C), and synthetic KL4 peptide were added to Survanta after extraction, creating extracted Survanta (ES) with 1% B, 2% B, and 2% B plus 1% C, or mixed with Survanta without extraction, creating modified Survanta (S) with 2% B, 2% B plus 1% C, and 2% KL4. Adult rats were ventilated with 100% oxygen, tidal volumes (VT) of 7.5 ml/kg and a rate of 60/min, and were lavaged until the PaO2 dropped below 80 mm Hg, when 100 mg/kg of surfactant was instilled. After 15 to 60 min of ventilation, pressure-volume (P-V) curves were generated in situ. Instillation of ES or S with 2% B plus 1% C led to the greatest increase in oxygenation, closely followed by ES and S with 2% B, and more distantly by S plus 2% KL4. TLC was comparable among the ES and S groups, but greater than that of air-placebo controls. These data suggest that spiking of Survanta with synthetic SP-B and SP-C increased oxygenation more effectively than B or KL4 alone in this surfactant-deficient rat model.
Walther
F J
FJ
Department of Pediatrics, Charles R. Drew University of Medicine and Science, Los Angeles, California, USA.
Hernández-Juviel
J
J
Bruni
R
R
Waring
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D001688:Biological Products; D001784:Blood Gas Analysis; D004195:Disease Models, Animal; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D006801:Humans; D006819:Hyaline Membrane Disease / Q000188:drug therapy* / Q000378:metabolism; D007231:Infant, Newborn; D007322:Instillation, Drug; D008297:Male; D008969:Molecular Sequence Data; D010100:Oxygen / Q000097:blood*; D011510:Proteolipids / Q000737:chemistry / Q000627:therapeutic use*; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001688:Biological Products; D004338:Drug Combinations; D011510:Proteolipids; D011663:Pulmonary Surfactants; C072197:beractant; D010100:Oxygen