title: Structure and chromosomal assignment of the mouse fra-1 gene, and its exclusion as a candidate gene for oc (osteosclerosis).
abstract: We have determined the genomic structure of the mouse fra-1 gene, which consists of four exons and three introns at positions also found in the other members of the fos gene family. Fra-1 is expressed rather highly in the brain and testes of adult mice, and at low levels in most other tissues. Absence of c-Fos leads to significantly reduced serum stimulation of fra-1 expression in gene targeted mouse fibroblasts, demonstrating that mitogen induction of fra-1 is partially mediated by c-Fos/AP-1. A polymorphic (CA)n microsatellite marker was found in intron 2 of fra-1 and used to map the gene to the centromeric region of mouse chromosome 19. Since fra-1 maps to the same genomic region as oc (osteosclerosis), an autosomal recessive disorder leading to the bone remodelling disease osteopetrosis, we tested it as a candidate gene for oc. The segregation of fra-1 in two different crosses of mice carrying oc and an allelism test between oc and a targeted disruption of fra-1 demonstrate that fra-1 and oc are two distinct genes rather than oc being a mutant allele of fra-1.
authors.affiliation: Research Institute of Molecular Pathology (IMP), Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Schreiber
authors.forename: C
authors.initials: C
authors.lastname: Poirier
authors.forename: A
authors.initials: A
authors.lastname: Franchi
authors.forename: R
authors.initials: R
authors.lastname: Kurzbauer
authors.forename: J L
authors.initials: JL
authors.lastname: Guenet
authors.forename: G F
authors.initials: GF
authors.lastname: Carle
authors.forename: E F
authors.initials: EF
authors.lastname: Wagner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005091:Exons; D015870:Gene Expression; D005796:Genes; D008297:Male; D051379:Mice; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010026:Osteosclerosis / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014018:Tissue Distribution; D000099169:Fos-Related Antigen 1
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D000099169:Fos-Related Antigen 1