Enantioselective analysis of praziquantel in plasma samples.
A direct enantioselective high-performance liquid chromatography method is described for the quantitative determination of praziquantel enantiomers in plasma samples. The method involves two-step extraction of plasma with toluene, evaporation of the solvent and chromatography on a Chiralcel OD-H column using hexane-ethanol (85:15, v/v) as the mobile phase and detection at 220 nm. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.
Jabor
V A
VA
Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Brazil.
Rocha
G M
GM
Bonato
P S
PS
D000818:Animals; D000980:Antiplatyhelmintic Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D011223:Praziquantel / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000980:Antiplatyhelmintic Agents; D011223:Praziquantel