The regulation of progesterone and hCG production from placental cells by interleukin-1beta.
We have investigated the roles of interleukin-1beta as a regulator of progesterone and chorionic gonadotrophin production from human placental cells. In primary placental cells IL-1beta increased hCG synthesis through a cyclic AMP-independent pathway, and was without effect on progesterone or cyclic AMP production. Since dibutyryl cyclic AMP increased progesterone production, this suggests that there is no coupling between the IL-1beta receptor and the adenylate cyclase enzyme in these cells. Immortalised trophoblast cells responded to IL-1beta by increasing progesterone production through a cyclic AMP-dependent mechanism, but hCG production by these cells was unaffected by IL-1beta or dibutyryl cyclic AMP. Further studies are needed to identify the role of IL-1beta as a possible regulator of progesterone production in primary placental cells. While hCG production in first-trimester trophoblast was increased by dibutyryl cyclic AMP and IL-1beta, both these effects may involve other factors such as IL-6, and their second messenger systems.
Seki
H
H
R.P.M.S. Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London, UK.
Zosmer
A
A
Elder
M G
MG
Sullivan
M H
MH
D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D011247:Pregnancy; D011374:Progesterone / Q000096:biosynthesis*; D014327:Trophoblasts / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D007375:Interleukin-1; D011374:Progesterone; D000242:Cyclic AMP