TEM moiré patterns explain STM images of bacteriophage T5 tails.
A subtle combination of constant current and constant height modes in scanning tunnelling microscopy allowed the imaging of a non-flat uncoated biological specimen, namely the tail of the bacteriophage T5. In parallel, a reference three-dimensional structure of the T5 tail was calculated from cryo-transmission electron microscopy images, based on its helical symmetry. This three dimensional reconstruction was compared with scanning tunnelling microscopy data. The images of the tail obtained by transmission electron microscopy, as well as projections of the reconstructed model, show similar moiré patterns. Here we show that scanning tunnelling microscopy performed in an aqueous environment provides direct images which are remarkably similar to the projection of the three dimensional model obtained by transmission electron microscopy. We deduce that our scanning tunnelling microscopy images are the result of a transmission of electrons through the gap between the scanning tip and the conductive support across the biological specimen.
Guénebaut
V
V
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Maaloum
M
M
Bonhivers
M
M
Wepf
R
R
Leonard
K
K
Hörber
J K
JK
D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D013604:T-Phages / Q000648:ultrastructure*; D014771:Virion / Q000648:ultrastructure