Use of a binary mixture of quaternary ammonium salts in fluorometric determination of glycosaminoglycans.
The concentration of glycosaminoglycans, in an aqueous solution was determined by observing the fluorescent intensity of the ion of 2-hexadecyl-9H-pyrido[4,3b]indole at 450 nm by irradiating 253-nm light after dissolution of the insoluble salt in ethanol, which was formed by the reaction between a solution of a sample of glycosaminoglycan and an aqueous solution of the binary mixture of fluorescent 2-hexadecyl-9H-pyrido[4,3b]indolium bromide and hexadecyl pyridinium chloride. The fluorescent quaternary ammonium salt, which was slightly soluble in water, was solubilized in water by forming mixed micelles with a nonfluorescent soluble quaternary ammonium salt. The present method showed good linearity for the calibration curves between 5 and 1000 micrograms/ml in all samples of glycosaminoglycan except keratan sulfate. The relative standard deviation in determination was less than 3% for the whole calibration range.
Kakehi
K
K
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-osaka, Japan. k_kakehi@phar.kindai.ac.jp
Maeya
Y K
YK
Miki
Y
Y
Oda
Y
Y
Hayase
S
S
D002809:Chondroitin Sulfates / Q000737:chemistry; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods*; D006025:Glycosaminoglycans / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D012996:Solutions; D013431:Sulfates / Q000737:chemistry
D005456:Fluorescent Dyes; D006025:Glycosaminoglycans; D000644:Quaternary Ammonium Compounds; D012996:Solutions; D013431:Sulfates; C012036:sodium sulfate; D002809:Chondroitin Sulfates