A novel MLL-AF10 fusion mRNA variant in a patient with acute myeloid leukemia detected by a new asymmetric reverse transcription PCR method.
A novel variant of the chimerical MLL-AF10 mRNA transcript was detected in a pediatric patient with acute myeloid leukemia (AML) by a new asymmetric reverse-transcription polymerase chain reaction (ART-PCR) method. Sequence analysis of the fusion region on the amplified cDNA fragment showed an in-frame joining of exon e5 of the MLL gene and position 1931 of the cDNA sequence of the AF10 gene, giving rise to a new MLL-AF10 transcript. The presence of the new chimerical mRNA product in a sample from the patient was confirmed by classical RT-PCR.
Hjorth-Sørensen
B
B
Institute of Molecular and Structural Biology, Aarhus University, Denmark.
Pallisgaard
N
N
Grönholm
M
M
Hokland
P
P
Clausen
N
N
Jørgensen
P
P
D000208:Acute Disease; D000595:Amino Acid Sequence; D002648:Child; D002879:Chromosomes, Human, Pair 10; D002880:Chromosomes, Human, Pair 11; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics*; D008969:Molecular Sequence Data; D051788:Myeloid-Lymphoid Leukemia Protein; D016133:Polymerase Chain Reaction / Q000379:methods*; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000235:genetics; D011993:Recombinant Fusion Proteins; D015183:Restriction Mapping; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C497422:KMT2A protein, human; C092513:MLLT10 protein, human; D012334:RNA, Neoplasm; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase