title: Retrovirus-induced target cell activation in the early phases of infection: the mouse mammary tumor virus model.
abstract: Mouse mammary tumor virus (MMTV) infects B lymphocytes and expresses a superantigen on the cell surface after integration of its reverse-transcribed genome. Superantigen-dependent B- and T-cell activation becomes detectable 2 to 3 days after infection. We show here that before this event, B cells undergo a polyclonal activation which does not involve massive proliferation. This first phase of B-cell activation is T cell independent. Moreover, during the first phase of activation, when only a small fraction of B cells is infected by MMTV(SW), viral DNA is detected only in activated B cells. Such a B-cell activation is also seen after injection of murine leukemia virus but not after injection of vaccinia virus, despite the very similar kinetics and intensity of the immune response. Since retroviruses require activated target cells to induce efficient infection, these data suggest that the early polyclonal retrovirus-induced target cell activation might play an important role in the establishment of retroviral infections.
authors.affiliation: Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Ardavin
authors.forename: F
authors.initials: F
authors.lastname: Luthi
authors.forename: M
authors.initials: M
authors.lastname: Andersson
authors.forename: L
authors.initials: L
authors.lastname: Scarpellino
authors.forename: P
authors.initials: P
authors.lastname: Martin
authors.forename: H
authors.initials: H
authors.lastname: Diggelmann
authors.forename: H
authors.initials: H
authors.lastname: Acha-Orbea
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology*; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D016679:Genome, Viral; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology / Q000821:virology; D008213:Lymphocyte Activation; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000821:virology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D008892:Milk / Q000821:virology; D012192:Retroviridae Infections / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D013997:Time Factors; D014412:Tumor Virus Infections / Q000276:immunology*; D016662:Virus Integration; D014779:Virus Replication
chemical_list: D004279:DNA, Viral