Intercellular adhesion molecule-1-deficient mice have antibody responses but impaired leukocyte recruitment.
The role of intercellular adhesion molecule-1 (ICAM-1) in murine lung inflammation was examined in vivo. Ovalbumin (Ova)-sensitized and -challenged ICAM-1-deficient (KO) mice had decreased accumulation of leukocytes in the bronchoalveolar lavage fluid compared with wild-type (WT) mice. Lung tissue inflammation was also attenuated. Ova immunization and challenge produced equivalent plasma levels of Ova-specific immunoglobulin (Ig) G1 and higher concentrations of IgE in KO versus WT mice. Ova-dependent induction of cytokines in vitro, as measured by enzyme-linked immunosorbent assay, was impaired in splenocytes from KO mice compared with the comparable release of interleukin (IL)-5 and IL-10 from anti-CD3-stimulated WT and KO splenocytes. Methacholine-induced increases in trapped gas in lungs of Ova-sensitized and -challenged WT mice were greater than those of KO mice. The activation of lung tissue nuclear factor-kappa B was diminished in KO mice after Ova provocation. This suggests that ICAM-1 was important for activation of the inflammatory cascade leading to the recruitment of leukocytes but was not critical for the generation of antibody responses in vivo.
Hatfield
C A
CA
Pharmacia and Upjohn, Kalamazoo, Michigan 49001, USA.
Brashler
J R
JR
Winterrowd
G E
GE
Bell
F P
FP
Griffin
R L
RL
Fidler
S F
SF
Kolbasa
K P
KP
Mobley
J L
JL
Shull
K L
KL
Richards
I M
IM
Chin
J E
JE
D000818:Animals; D000917:Antibody Formation; D000943:Antigens, Differentiation / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016207:Cytokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000502:physiology*; D007962:Leukocytes / Q000276:immunology / Q000502:physiology*; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000502:physiology*; D016210:Methacholine Chloride / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016328:NF-kappa B / Q000378:metabolism; D010047:Ovalbumin; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000943:Antigens, Differentiation; D016207:Cytokines; D007074:Immunoglobulin G; D016328:NF-kappa B; D016210:Methacholine Chloride; D018799:Intercellular Adhesion Molecule-1; D007073:Immunoglobulin E; D010047:Ovalbumin