Influence of cultural conditions on lipid production by mutant strain of Rhodotorula glutinis MTCC 1151.
Mutant of Rhodotorula glutinis MTCC 1151 was found to produce high level of lipid (63.6% of biomass) as compared to parent strain (56.7% biomass). The lipid synthesizing capacity of mutant of R. glutinis was evaluated with different glucose concentrations, nitrogen sources, incubation time, and aeration-agitation. Maximum lipid yield (63.6% of biomass) was found with 5% glucose using ammonium sulfate (0.2%) as a nitrogen source under shake-flask conditions (50 ml broth in 250 ml conical flask at 125 rpm) after 4 days of incubation at 28 degrees C. The ability of ammonium sulfate to replace comparatively very costly yeast extract is highly appreciable.
Pathak
R
R
Department of Microbiology, University School of Sciences, Gujarat University, Ahmedabad, India.
Yadav
N K
NK
D000332:Aerobiosis; D000645:Ammonium Sulfate / Q000378:metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D009154:Mutation; D012248:Rhodotorula / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D008055:Lipids; D005947:Glucose; D000645:Ammonium Sulfate