title: Vitamin E supplementation in elderly lowers the oxidation rate of linoleic acid in LDL.
abstract: Oxidation of LDL-linoleic acid (LDL-LA), a major substrate for lipid peroxidation, may be counteracted by the antioxidant vitamin E. In a 3-month randomized double-blind placebo-controlled trial in 83 apparently healthy Dutch elderly, aged 67-85 years, the direct protective effect of 100 IU vitamin E on the rate of oxidized LDL-LA was studied. The oxidation of LDL-LA was measured by its disappearance after a 5-h in vitro Cu-oxidation of LDL isolated from 1 ml plasma. In the vitamin E group, the decrease in oxidized LDL LA of 10.4, (p < 0.05) was significantly different (p < 0.05) from the smaller 4.6% p< 0.01) decrease in the control group. Moreover, within the vitamin E group the decrease was even more marked over tertiles of alpha-tocopherol to LDL-LA ratio with a significant difference in decrease (p < 0.05) from the lowest compared to the highest tertile of, respectively, 18.4% [-24; -2%] (median and range) and 2.0% [-16: 34%]. In conclusion, supplementation with 100 IU vitamin E in elderly is beneficial in lowering the rate of oxidation of LDL LA. The protective effect of vitamin E might best be monitored by using the ratio of alpha-tocopherol to LDL-LA as this reflects the degree of alpha-tocopherol available to protect the amount of LDL-LA present.
authors.affiliation: Department of Epidemiology and Public Health, Wageningen Agricultural University, The Netherlands. frouwkje.de waart@medew.hegl.wau.nl
authors.forename: F G
authors.initials: FG
authors.lastname: de Waart
authors.forename: U
authors.initials: U
authors.lastname: Moser
authors.forename: F J
authors.initials: FJ
authors.lastname: Kok
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000008:administration & dosage*; D001055:Apolipoproteins B / Q000097:blood / Q000187:drug effects; D016718:Arachidonic Acid / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D004311:Double-Blind Method; D005227:Fatty Acids / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005260:Female; D005527:Food, Fortified; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009426:Netherlands; D010084:Oxidation-Reduction / Q000187:drug effects*; D014810:Vitamin E / Q000008:administration & dosage* / Q000097:blood
chemical_list: D000975:Antioxidants; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D008055:Lipids; D008077:Lipoproteins, LDL; D014810:Vitamin E; D016718:Arachidonic Acid; D019787:Linoleic Acid