Effect of heparin on infection of cells by equine arteritis virus.
The number of plaques formed by equine arteritis virus (EAV) and Aujesky's disease virus (ADV) was reduced to 14% and 5% of the untreated control (100%), respectively, by 10 U/ml of heparin, but could not be reduced below to 13 and 4%, respectively, by use of concentration up to 100 U/ml. An inhibitory effect of heparin, at concentration up to 100 U/ml, was not observed on parainfluenza virus 3 (PIV-3). Heparinase treatment of RK13 cells reduced the number of EAV-, as well as ADV-induced plaques. On the other hand, the number of PIV-3 induced plaques did not decrease after treatment of RK13 cells with heparinase.
Asagoe
T
T
Department of Veterinary Epizootiology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
Inaba
Y
Y
Jusa
E R
ER
Kouno
M
M
Uwatoko
K
K
Fukunaga
Y
Y
D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D007240:Equartevirus / Q000187:drug effects / Q000254:growth & development*; D006493:Heparin / Q000494:pharmacology*; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000254:growth & development*; D006736:Horses; D007668:Kidney; D011817:Rabbits; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
D006493:Heparin