Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase.
The nifV gene product (NifV) from Azotobacter vinelandii was recombinantly expressed at high levels in Escherichia coli and purified. NifV is a homodimer that catalyzes the condensation of acetyl coenzyme A (acetyl-CoA) and alpha-ketoglutarate. Although alpha-ketoglutarate supports the highest level of activity, NifV will also catalyze the condensation of acetyl-CoA and certain other keto acids. E. coli cells in which a high level of nifV expression is induced excrete homocitrate into the growth medium.
Zheng
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Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg 24061-0346, USA.
White
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Dean
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D016948:Azotobacter vinelandii / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D019281:Dimerization; D008401:Gas Chromatography-Mass Spectrometry; D007652:Oxo-Acid-Lyases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
C021365:homocitrate synthase; D007652:Oxo-Acid-Lyases