title: Three classes of mammalian transcription activation domain stimulate transcription in Schizosaccharomyces pombe.
abstract: Representatives of three distinct classes of mammalian protein domain activating RNA polymerase II were fused to the yeast GAL4p DNA-binding domain. The resulting fusion proteins were tested in the fission yeast Schizosaccharomyces pombe for their ability to activate transcription of different reporter constructs containing GAL4-binding sites in positions close to or far from the TATA box. The acidic-rich activation domain of VP16 stimulates transcription in S.pombe from proximal and distal positions, suggesting that the mechanism of activation is conserved from man to budding and fission yeasts. Unlike in Saccharomyces cerevisiae, the glutamine-rich activation domains of Sp1, Oct1 and Oct2 activate transcription in S. pombe when tested in a proximal TATA box context. Similarly to mammalian cells, these domains are inactive or weakly active when tested in a distal position. Moreover, the proline-rich activation domains of AP-2 and CTF/NF1 display strong transcriptional activities from a TATA box-proximal position, and weak activities when tested in a remote position. Consequently, proline-rich and glutamine-rich activation domains act differently in S.cerevisiae and mammalian cells, but similarly in S.pombe and mammalian cells.
authors.affiliation: Department of Cell Growth, Differentiation and Development (VIB-07), Flanders Interuniversity Institute for Biotechnology, Leuven, Belgium. jre@sgi.celgen.kuleuven.ac.be
authors.forename: J E
authors.initials: JE
authors.lastname: Remacle
authors.forename: G
authors.initials: G
authors.lastname: Albrecht
authors.forename: R
authors.initials: R
authors.lastname: Brys
authors.forename: G H
authors.initials: GH
authors.lastname: Braus
authors.forename: D
authors.initials: D
authors.lastname: Huylebroeck
mesh_terms: D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000378:metabolism; D005973:Glutamine; D006801:Humans; D008322:Mammals; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000254:growth & development / Q000378:metabolism*; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; C060292:PHO4 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005973:Glutamine