title: Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I.
abstract: The Corynebacterium glutamicum glutamine synthetase I (GSI) structural gene glnA was cloned by a PCR approach using oligonucleotide primers derived from conserved amino acid sequences of the GSI proteins from various bacteria. Disruption or deletion of this gene in C. glutamicum led to a glutamine auxotrophic phenotype and complete loss of glutamine synthetase activity, indicating the key role of this enzyme in nitrogen metabolism. Additionally, indications for a second glutamine synthetase, GSII, were found.
authors.affiliation: Forschungszentrum Jülich, Institut für Biotechnologie I, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Jakoby
authors.forename: M
authors.initials: M
authors.lastname: Tesch
authors.forename: H
authors.initials: H
authors.lastname: Sahm
authors.forename: R
authors.initials: R
authors.lastname: Krämer
authors.forename: A
authors.initials: A
authors.lastname: Burkovski
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000201:enzymology* / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D017353:Gene Deletion; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009584:Nitrogen / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D000596:Amino Acids; D004269:DNA, Bacterial; C083818:glutamine synthetase 2; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen