IE2 protein is insufficient for transcriptional repression of the human cytomegalovirus US3 promoter.
Expression of the human cytomegalovirus (HCMV) US3 gene is regulated in part by transcriptional repression mediated through a cis-repressive region located between the TATA box and the transcriptional start site. The US3 cis-repressive element has extensive sequence identity with a similar repressive element in UL122-123 (the major immediate-early gene complex). Repression of UL122-123 is mediated through the interaction of the IE2 protein with cis-repressive sequences. In spite of the similarity of the two repressive elements, IE2 activated rather than repressed transcription from the US3 promoter. Additionally, IE1 or IE1 and IE2 in combination also activated US3 expression. These data demonstrate that regulation of HCMV immediate-early genes is quite complex and involves a number of proteins.
Biegalke
B J
BJ
Department of Biological Sciences, College of Osteopathic Medicine, Ohio University, Athens 45701, USA. biegalke@ohiou.edu
D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early; D006023:Glycoproteins; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D016385:TATA Box; D015534:Trans-Activators / Q000378:metabolism; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins; D014764:Viral Proteins
D006023:Glycoproteins; C088645:IE1 protein, cytomegalovirus; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C077406:UL115 protein, Human herpesvirus 5; C095111:US3 protein, cytomegalovirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus; C439068:glycoprotein O, cytomegalovirus