title: Gene transfer using replication-defective human foamy virus vectors.
abstract: Replication-defective vectors based on an infectious molecular clone of human foamy virus (HFV) were constructed by deletion and replacement of the accessory genes with expression cassettes for puromycin-resistance and beta-glucouronidase. Cell lines which produced in excess of 10(5) helper virus-free transducing units/ml were generated by trans-complementation of the replication defect using a BHK-21-derived cell line expressing the Bel-1 transactivator. Vectors based on the HFV genome may provide useful alternatives to existing retroviral vectors.
authors.affiliation: Department of G. U. Medicine and Communicable Diseases, Imperial College School of Medicine at St. Mary's, Paddington, London, United Kingdom.
authors.forename: P D
authors.initials: PD
authors.lastname: Bieniasz
authors.forename: O
authors.initials: O
authors.lastname: Erlwein
authors.forename: A
authors.initials: A
authors.lastname: Aguzzi
authors.forename: A
authors.initials: A
authors.lastname: Rethwilm
authors.forename: M O
authors.initials: MO
authors.lastname: McClure
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D003673:Defective Viruses; D004352:Drug Resistance, Microbial; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000096:biosynthesis; D006801:Humans; D007668:Kidney; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012191:Retroviridae Proteins / Q000096:biosynthesis*; D016092:Spumavirus; D015534:Trans-Activators / Q000096:biosynthesis*; D014162:Transfection / Q000379:methods; D014779:Virus Replication
chemical_list: D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012191:Retroviridae Proteins; D015534:Trans-Activators; C074061:bel1 protein, Human foamy virus; D005966:Glucuronidase