Lipochitooligosaccharide-induced tobacco cells release a peptide as mediator of the glycolipid signal.
Lipochitooligosaccharides (LCOs) are plant growth regulators that promote at subfemtomolar concentrations cell division in tobacco protoplasts. In response to LCO treatment, tobacco cells release a second growth factor that fully mediates the growth-promoting activities of the initial extracellular LCO stimulus. This diffusible growth factor was isolated from the protoplasts' culture filtrate and shown to be a peptide. We report that the LCO-induced mitogen released by tobacco cells and a synthetic heptadecapeptide derived from region 2 of the tobacco homolog of the early nodulin gene ENOD40 are antigenically related and qualitatively indistinguishable in their ability to stimulate cell division.
John
M
M
Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Cologne, Germany. John@mpiz-koeln.mpg.de
Schmidt
J
J
Walden
R
R
Czaja
I
I
Dülz
M
M
Schell
J
J
Röhrig
H
H
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary; D006017:Glycolipids / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008934:Mitogens / Q000378:metabolism; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics; D010947:Plants, Toxic; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D018076:DNA, Complementary; D006017:Glycolipids; D008070:Lipopolysaccharides; D008934:Mitogens; D010937:Plant Growth Regulators; D010940:Plant Proteins; C023023:lipid-linked oligosaccharides