An experimental study on application of PCR in detection of kala-azar.
A polymerase chain reaction (PCR) technique was developed to detect Kala-azar. Out of 25 patients, 24 were detected, a sensitivity of 96%. The specificity rate, false positive rate, false negative rate, coincidence rate and Youdent's index were 100% (60/60), 0, 4%, 99% and 0.96 respectively. It was shown that the PCR technique can directly detect Kala-azar in samples (1 microliter) from peripheral blood.
Wu
Z
Z
Jiangsu Institute of Parasitic Diseases, Wuxi, People Republic of China.
Bao
Y
Y
Ding
Y
Y
Yu
M
M
Lu
L
L
Zhang
Y
Y
D000818:Animals; D002681:China; D016054:DNA, Protozoan / Q000235:genetics*; D006801:Humans; D007893:Leishmania donovani / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000469:parasitology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D016054:DNA, Protozoan