title: Isolation and characterization of chicken beta-catenin.
abstract: beta-catenin interacts with a number of proteins in different important biological processes, including cell adhesion through cadherins, actin organization through fascin, body axis determination through Wnt signaling, tumor suppression through APC, and transcriptional activation through LEF-1. To examine its function in chicken embryogenesis, we isolated the chicken homolog of beta-catenin from a chicken embryo cDNA library. The sequence is highly conserved at the amino acid level between chicken, mouse (99%), human (99%) and Xenopus (97%). In-situ hybridization and immunostaining showed that in the developing limb, it is specifically expressed in the apical ectodermal ridge, suggesting a role in epithelial-mesenchymal interactions.
authors.affiliation: Department of Pathology, School of Medicine, University of Southern California, Los Angeles, USA.
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.forename: C M
authors.initials: CM
authors.lastname: Chuong
authors.forename: R B
authors.initials: RB
authors.lastname: Widelitz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002642:Chick Embryo; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism*; D004475:Ectoderm / Q000378:metabolism; D005121:Extremities / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D015534:Trans-Activators; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; C495267:CTNNB1 protein, mouse; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin