title: Cerebellar granule cell membranes and glutamate mediates transactivation of glutamine synthetase promoter.
abstract: Potential region of glutamine synthetase promoter driving astrocyte-specific transactivation, mediated by cerebellar granule cell membrane and glutamate has been identified by deletion analysis of promoter and transient transfection. The promoter region from -420 to -765 was found to be potentially important for this transactivation. These results provided further evidence for importance of neuronal-glial and glutamate-glial interactions in regulation of glial gene expression.
authors.affiliation: Laboratory of Molecular Biology, National Institute of Neurological Disorders and Strokes, National Institute of Health, Bethesda, MD 20892, USA. vishramK@qimr.edu.au
authors.forename: V
authors.initials: V
authors.lastname: Kedar
authors.forename: H
authors.initials: H
authors.lastname: Purohit
authors.forename: E
authors.initials: E
authors.lastname: Freeze
authors.forename: F
authors.initials: F
authors.lastname: Hempel
mesh_terms: D001253:Astrocytes / Q000166:cytology / Q000201:enzymology; D002531:Cerebellum / Q000166:cytology* / Q000201:enzymology; D003594:Cytoplasmic Granules / Q000502:physiology*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D018698:Glutamic Acid / Q000502:physiology*; D006801:Humans; D007425:Intracellular Membranes / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D009474:Neurons / Q000166:cytology / Q000201:enzymology; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
chemical_list: D018698:Glutamic Acid; D005974:Glutamate-Ammonia Ligase