Reconstitution of the protein insertion machinery of the mitochondrial inner membrane.
We have reconstituted the protein insertion machinery of the yeast mitochondrial inner membrane into proteoliposomes. The reconstituted proteoliposomes have a distinct morphology and protein composition and correctly insert the ADP/ATP carrier (AAC) and Tim23p, two multi-spanning integral proteins of the mitochondrial inner membrane. The reconstituted system requires a membrane potential, but not Tim44p or mhsp70, both of which are required for the ATP-driven translocation of proteins into the matrix. The protein insertion machinery can thus operate independently of the energy-transducing Tim44p-mhsp70 complex.
Haucke
V
V
Department of Biochemistry, Biozentrum, University of Basel, Switzerland. volker.haucke@yale.edu
Schatz
G
G
D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D002704:Chlorates / Q000378:metabolism; D003902:Detergents; D004058:Diffusion; D004734:Energy Metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D007074:Immunoglobulin G / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D026901:Membrane Transport Proteins; D008928:Mitochondria / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D011510:Proteolipids / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D012995:Solubility
D002352:Carrier Proteins; D002704:Chlorates; D003902:Detergents; D005656:Fungal Proteins; D007074:Immunoglobulin G; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D011510:Proteolipids; D029701:Saccharomyces cerevisiae Proteins; C083081:TIM23 protein, S cerevisiae; C020485:proteoliposomes; D000226:Mitochondrial ADP, ATP Translocases