Regionally different effects of scopolamine on NMDA antagonist-induced heat shock protein HSP70.
Using immunohistochemical technique, we investigated the regionally different roles of muscarinic receptors in the induction of HSP-70 by NMDA receptor antagonists. The administration of memantine and phencyclidine induced HSP-70 in the retrosplenial cortex of rat brain. Pretreatment with the muscarinic receptor antagonist scopolamine (0.1-1 mg/kg) blocked induction of HSP-70 in layer III of the retrosplenial cortex. However, induction of HSP-70 in layer V was augmented by scopolamine. These results suggest a regional difference in the mechanism of neurotoxicity induced by NMDA receptor antagonists.
Tomitaka
S I
SI
Department of Cortical Function Disorder, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
Hashimoto
K
K
Narita
N
N
Minabe
Y
Y
Tamura
A
A
D000818:Animals; D000918:Antibody Specificity; D002540:Cerebral Cortex / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008559:Memantine / Q000494:pharmacology; D018727:Muscarinic Antagonists / Q000494:pharmacology*; D010622:Phencyclidine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors*; D012601:Scopolamine / Q000494:pharmacology*
D018691:Excitatory Amino Acid Antagonists; D018840:HSP70 Heat-Shock Proteins; D018727:Muscarinic Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; D012601:Scopolamine; D010622:Phencyclidine; D008559:Memantine