Apolipoprotein B (apoB) concentrations in lipoproteins in cows.
The concentrations of apolipoprotein B (apoB)-48 and apoB-100 in triglyceride-rich lipoproteins (TRL), intermediate density lipoprotein (IDL), and low density lipoprotein (LDL) fractions separated by gel permeation chromatography were determined in Holstein and Japanese black cows by enzyme-linked immunosorbent assay (ELISA). A significant correlation (p < 0.01) was observed between apoB-48 in TRL and plasma triglyceride (TG) levels in both Holstein and Japanese black cows. Additionally, apoB-48 in TRL and plasma TG levels in Holstein cows were significantly lower (p < 0.01) than those in Japanese black cows. These results suggested that TG derived from intestinal (exogenous) TRL rather than from liver (endogenous) TRL was the major source of milk fat.
Uchide
T
T
Department of Veterinary Clinical Pathobiology, Faculty of Agriculture, University of Tokyo, Japan.
Tohya
Y
Y
Onda
K
K
Matsuki
N
N
Inaba
M
M
Ono
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000097:blood*; D002417:Cattle / Q000097:blood*; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007564:Japan; D008074:Lipoproteins / Q000097:blood*; D053419:Lipoproteins, IDL; D008077:Lipoproteins, LDL / Q000097:blood; D013045:Species Specificity; D014280:Triglycerides / Q000097:blood
D000911:Antibodies, Monoclonal; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D008074:Lipoproteins; D053419:Lipoproteins, IDL; D008077:Lipoproteins, LDL; D014280:Triglycerides