Cloning of a cDNA encoding a novel sugar transporter expressed in the neonatal mouse hippocampus.
While analyzing active genes in the neonatal mouse hippocampus, we observed several novel genes that were abundantly expressed in this tissue. We report here cloning and sequencing of one of these transcripts, HiAT1 (Hippocampus Abundant Gene Transcript 1). The mRNA was 2.7 Kb in length, and the deduced amino acid sequence consisted of 490 amino acids with characteristics typical of members of the sugar transporter family. However, its overall sequence homology to known transporter cDNAs was only about 30%, suggesting strongly that it represents a novel sugar transporter gene. Northern hybridization analyses showed this transcript is detected in adult and embryonic brains, as well as in other tissues.
Matsuo
N
N
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Kawamoto
S
S
Matsubara
K
K
Okubo
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000302:isolation & purification*; D018507:Gene Expression Regulation, Developmental; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D009928:Organ Specificity; D014158:Transcription, Genetic
D018076:DNA, Complementary; C108259:HiAT1 transporter; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins