title: Study of the adjuvant activity of new MDP derivatives and purified saponins and their influence on HIV-1 replication in vitro.
abstract: Muramyl dipeptide (MDP), eight new lipophilic MDP derivatives (MDPs) and three purified saponins were evaluated for their ability to induce immune responses in mice immunized with HIV-1 envelope protein rgp160 and for their ability to influence the HIV-1 replication in vitro. Three of nine new synthetic MDP derivatives (beta-butyl-MDP, MTPO-26 and beta-cholesteryl-MDP) and one saponin (Taurosid I) have been shown to induce strong humoral immune responses to HIV-1 envelope glycoproteins rgp160 and rgp120. Three substances (beta-butyl-MDP, MDP-cholyl and beta-G27-MDP) induced high levels of T-cell stimulation to HIV-1 rgp160. beta-butyl-MDP induced the strongest B- and T-cell responses to HIV-1 glycoproteins. Two substances (beta-butyl-MDP and Taurosid I) did not induce an enhancement of HIV-1 replication in vitro and can be considered as promising adjuvants.
authors.affiliation: Department of Microbiology and Virology, Crimean Medical Institute, Ukraine.
authors.forename: Y L
authors.initials: YL
authors.lastname: Krivorutchenko
authors.forename: I B
authors.initials: IB
authors.lastname: Andronovskaja
authors.forename: J
authors.initials: J
authors.lastname: Hinkula
authors.forename: Y S
authors.initials: YS
authors.lastname: Krivoshein
authors.forename: E
authors.initials: E
authors.lastname: Ljungdahl-Ståhle
authors.forename: S S
authors.initials: SS
authors.lastname: Pertel
authors.forename: V I
authors.initials: VI
authors.lastname: Grishkovets
authors.forename: A E
authors.initials: AE
authors.lastname: Zemlyakov
authors.forename: B
authors.initials: B
authors.lastname: Wahren
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives / Q000494:pharmacology*; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D005260:Female; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012503:Saponins / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000276:Adjuvants, Immunologic; D012503:Saponins; D000119:Acetylmuramyl-Alanyl-Isoglutamine