Induction of the rat hepatic cytosolic arylamine oxidase by a series of polychlorinated biphenyls: association with the Ah locus.
The objective of the present study was to identify the polychlorinated biphenyl congeners of Aroclor 1254 that are responsible for the induction of the cytosolic bioactivation of aromatic amines. Various chlorobiphenyls, ranging from di- to hexa-substituted, were administered to rats and the ability of the hepatic cytosol to bioactivate 2-aminoanthracene and 2-aminofluorene was investigated. These studies revealed that the induction of the cytosolic activation of aromatic amines increased with the increasing extent of chlorination; moreover, planar congeners were more effective inducers of this activity compared to their non-planar isomers. This observation prompted us to investigate whether the cytosolic activation of aromatic amines is associated with the Ah receptor. Treatment of mice with Aroclor 1254 stimulated the cytosolic activation of aromatic amines in C57BL6 mice, an Ah-responsive strain, whereas it had no effect in DBA2 mice, a non-responsive strain. These findings indicate that the bioactivation of aromatic amines by the liver cytosol is linked to the Ah receptor.
Marczylo
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T
Molecular Toxicology Group, School of Biological Sciences, University of Surrey, Guildford, UK.
Ioannides
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C
D000818:Animals; D001140:Aroclors / Q000378:metabolism / Q000633:toxicity*; D001191:Arylamine N-Acetyltransferase / Q000096:biosynthesis*; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D020111:Chlorodiphenyl (54% Chlorine); D003600:Cytosol / Q000187:drug effects* / Q000201:enzymology; D004790:Enzyme Induction; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017208:Rats, Wistar; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*
D001140:Aroclors; D002273:Carcinogens; D018336:Receptors, Aryl Hydrocarbon; D020111:Chlorodiphenyl (54% Chlorine); D001191:Arylamine N-Acetyltransferase