Effects of horminone on liver mixed function mono-oxygenases and glutathione enzyme activities of Wistar rat.
The present study reports on the effects of horminone on serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, on hepatic cytochrome P450 (P450) and cytochrome b5 (cyt b5) contents and on the activities of NADPH-cytochrome P450 reductase (NR), mixed function mono-oxygenases (MFO), glutathione-S-transferase (GST) and glutathione reductase (GR) of Wistar male rat. Horminone is a diterpenoid quinone (7,12-dihydroxyabiet-8,12-diene-11,14-dione) present in several species of the Labiatae family and used as medicinal plants in folk medicine. In this study, horminone was administered by the intraperitoneal route (i.p.) at a concentration of 1 or 10 mg/kg to each group of six mice, using water as a vehicle. On the one hand, results showed that horminone increased serum ALT and AST levels and cyt b5 content and induced the activities of ethylmorphine N-demethylase (EMD). On the other hand, horminone decreased P450 content and inhibited the activities of 7-ethoxyresorufin O-deethylase (ERD), 7-ethoxycoumarin O-deethylase (ECD), aniline 4-hydroxylase (AH) and NR. Based on these results, the possibility of toxic effects occurring after administration of plant extracts containing horminone must be considered.
Ferreira
R
R
Department of Chemistry, University of Evora, Portugal.
Candeias
F
F
Simões
F
F
Nascimento
J
J
Cruz Morais
J
J
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D045784:Abietanes; D000410:Alanine Transaminase / Q000097:blood; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000097:blood; D015786:Cytochromes b5 / Q000097:blood; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005037:Ethylmorphine-N-Demethylase / Q000096:biosynthesis; D005980:Glutathione Reductase / Q000097:blood*; D005982:Glutathione Transferase / Q000097:blood*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D006899:Mixed Function Oxygenases / Q000097:blood*; D009251:NADPH-Ferrihemoprotein Reductase / Q000097:blood; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar
D045784:Abietanes; D004224:Diterpenes; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D015660:7-Alkoxycoumarin O-Dealkylase; D000815:Aniline Hydroxylase; D019363:Cytochrome P-450 CYP1A1; D005037:Ethylmorphine-N-Demethylase; D009251:NADPH-Ferrihemoprotein Reductase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C045490:horminone