Direct determination of prolintane and its metabolite oxoprolintane in human urine by capillary zone electrophoresis and beta-cyclodextrin-modified micellar electrokinetic chromatography.
This paper describes a fast and simple method for the direct determination of the stimulant prolintane and its principal urinary metabolite, oxoprolintane, in human urine by capillary zone electrophoresis and beta-cyclodextrin-modified micellar electrokinetic chromatography. The determination was performed in phosphate buffer, pH 8.5, with UV detection at 211 nm. The effect of the ionic strength ratio between sample and running electrolyte, pH, sodium dodecyl sulphate and beta-cyclodextrin concentrations, and other factors, on the electrophoretic signals of these drugs was examined. This method can be applied for doping control. The determination limits are 1.0 microgram ml-1 for prolintane and 0.7 microgram ml-1 for oxoprolintane.
Espartero
A G
AG
Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid, Spain.
Pérez
J A
JA
Zapardiel
A
A
Bermejo
E
E
Hernández
L
L
D000697:Central Nervous System Stimulants / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D003505:Cyclodextrins / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D008823:Micelles; D009994:Osmolar Concentration; D011759:Pyrrolidines / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D011760:Pyrrolidinones / Q000737:chemistry / Q000652:urine*; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000697:Central Nervous System Stimulants; D003505:Cyclodextrins; D008823:Micelles; D011759:Pyrrolidines; D011760:Pyrrolidinones; C003024:oxoprolintane; D012967:Sodium Dodecyl Sulfate; C005598:prolintane