Interferon-beta prevents antigen-induced bronchial inflammation and airway hyperreactivity in mice.
The effects of IFN-beta and prednisolone (Pred) on antigen-induced IgE antibody production, airway eosinophilia and airway hyperreactivity (AHR) were studied in ovalbumin-sensitized Balb/c mice. Three inhalations of antigen (ovalbumin) caused an increase in the number of eosinophils and lymphocytes in bronchoalveolar lavage fluid and AHR to acetylcholine with a significant elevation in the serum IgE level. IFN-beta clearly inhibited the antigen-induced airway inflammation and AHR, but did not affect IgE antibody production. Pred inhibited antigen-induced IgE antibody production, airway inflammation and AHR to acetylcholine. In addition, IFN-beta inhibited T-helper type 2 (Th2) cell clone (D10G4.1)-induced peritoneal eosinophilia in mice, but did not affect neutrophilia, whereas Pred inhibited D10G4.1-induced peritoneal eosinophilia and neutrophilia. These results suggest that IFN-beta inhibits antigen-induced bronchial inflammation and AHR probably due to the inhibition of Th2-induced airway eosinophilia.
Maeda
Y
Y
Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Musoh
K
K
Shichijo
M
M
Tanaka
H
H
Nagai
H
H
D000109:Acetylcholine / Q000008:administration & dosage; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D000280:Administration, Inhalation; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000188:drug therapy*; D001991:Bronchitis / Q000188:drug therapy*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D016899:Interferon-beta / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D007958:Leukocyte Count / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage; D010529:Peritoneal Cavity / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D016899:Interferon-beta; D010047:Ovalbumin; D000109:Acetylcholine