Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry.
Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms.
Imbert-Marcille
B M
BM
Virology Laboratory, Institute of Biology of Nantes University Hospital, France. bmimbert@sante.univ-nantes.fr
Robillard
N
N
Poirier
A S
AS
Coste-Burel
M
M
Cantarovich
D
D
Milpied
N
N
Billaudel
S
S
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000097:blood*; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects; D009504:Neutrophils / Q000821:virology*; D010539:Permeability; D015203:Reproducibility of Results; D013997:Time Factors; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral