Deconvolution analysis of bioassayable LH secretion and half-life in men with idiopathic oligoasthenospermia.
To further investigate the nature of neuroendocrine disturbances of the hypothalamopituitary-gonadal axis in idiopathic male infertility, we studied 12 infertile men with oligoasthenozoospermia and 13 euspermic controls, matched for age and body mass index, by blood withdrawal at 10-min intervals for 8 h to analyse pulsatile release of bioactive LH (b-LH). The rat interstitial cell testosterone (RICT) bioassay was used in conjunction with a recently validated multiparameter deconvolution algorithm, to estimate the endogenous half-life of b-LH, its secretory burst frequency, amplitude, duration and mass. Oligoasthenospermic men exhibited significant (p < 0.05) alterations within the LH axis; namely: (1) a prolonged half-life of b-LH (92 min in euspermic men, 127 min in oligoasthenospermic men); (2) a reduced b-LH secretory burst amplitude (2.2 +/- 1.2 IU/l/min in euspermic men, 1.7 +/- 0.8 IU/l/min in oligoasthenospermic men); (3) a lower bioactive/immunoactive (b/i) ratio for LH secretory burst amplitude (14 in euspermic men, 4 in oligoasthenospermic men); (4) a reduced b/i ratio in the mass of LH secreted per burst (5.4 in euspermic men, 4.1 in oligoasthenospermic men) and (5) decreased coordinate release of b-LH and testosterone in infertile men, as assessed by cross-correlation analysis. These disturbances differ from the neuroendocrine dysregulation described in other states of male hypogonadotrophism.
Reyes-Fuentes
A
A
División de Investigación Biomédica, Instituto Nacional de Perinatología, México D.F., México.
Chavarría
M E
ME
Aguilera
G
G
Rosado
A
A
Iranmanesh
A
A
Veldhuis
J D
JD
D000204:Activity Cycles; D000328:Adult; D000465:Algorithms; D000818:Animals; D001681:Biological Assay / Q000379:methods; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood / Q000172:deficiency / Q000378:metabolism*; D008297:Male; D008596:Menotropins / Q000494:pharmacology; D009845:Oligospermia / Q000097:blood / Q000503:physiopathology*; D011863:Radioimmunoassay; D051381:Rats; D012016:Reference Values; D015203:Reproducibility of Results; D013739:Testosterone / Q000378:metabolism
D013739:Testosterone; D008596:Menotropins; D007986:Luteinizing Hormone