title: Helicobacter pylori lipopolysaccharide stimulates histamine release and DNA synthesis in rat enterochromaffin-like cells.
abstract: OBJECTIVE
  Helicobacter pylori alterations in gastric acid output and mucosal proliferation may involve the enterochromaffin-like (ECL) cell. To test whether H. pylori affects ECL cell histamine secretion and proliferation, the effect of lipopolysaccharide (LPS) on ECL cell function in vitro was investigated.
  
  
  METHODS
  Using a rat ECL cell preparation of high purity (+/-95%), basal and stimulated histamine secretion and DNA synthesis were measured by enzyme immunoassay and bromodeoxyuridine (BrdU) uptake, respectively.
  
  
  RESULTS
  Escherichia coli LPS (10(-12) to 10(-6) mol/L) had no effect on basal and stimulated histamine secretion at concentrations of > 10(-6) mol/L. H. pylori LPS stimulated basal and gastrin-stimulated histamine secretion. These effects were completely inhibited by somatostatin (10(-10) mol/L) but not by the gastrin receptor antagonist L365,260 at 10(-6) mol/L. E. coli LPS had a weak stimulatory effect on ECL cell BrdU uptake at 10(-6) mol/L but had no effect on gastrin-stimulated BrdU uptake. H. pylori LPS did not stimulate basal synthesis but significantly increased (1.5-fold) gastrin-stimulated BrdU uptake.
  
  
  CONCLUSIONS
  H. pylori influences both ECL cell proliferation and secretion in vitro. An interaction between H. pylori LPS and ECL cells may contribute to the reported abnormalities in acid secretion and gastric pathobiology noted in H. pylori infections.
authors.affiliation: Gastrointestinal Surgical Pathobiology Research Group, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kidd
authors.forename: K
authors.initials: K
authors.lastname: Miu
authors.forename: L H
authors.initials: LH
authors.lastname: Tang
authors.forename: G I
authors.initials: GI
authors.lastname: Perez-Perez
authors.forename: M J
authors.initials: MJ
authors.lastname: Blaser
authors.forename: A
authors.initials: A
authors.lastname: Sandor
authors.forename: I M
authors.initials: IM
authors.lastname: Modlin
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004759:Enterochromaffin Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004926:Escherichia coli; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology*; D005755:Gastrins / Q000494:pharmacology; D016480:Helicobacter pylori; D006636:Histamine Release / Q000187:drug effects / Q000502:physiology*; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005755:Gastrins; D008070:Lipopolysaccharides; D004247:DNA; D001973:Bromodeoxyuridine