Dominant nature of the resistance to Fas- and tumor necrosis factor-alpha-mediated apoptosis in human prostatic carcinoma cell lines.
We have recently found (O. W. Rokhlin et al., Cancer Res., 57: 1758-1768, 1997) that, although Fas ligation induced apoptosis in two of six human prostatic carcinoma cell lines investigated, the apoptotic machinery involved in Fas-mediated killing is already in place in Fas-resistant cell lines. Here, we investigated Fas- and tumor necrosis factor-alpha (TNF-alpha)-mediated apoptosis in cell hybrids between resistant (DU145 and JCA1) and sensitive (ALVA31 and PC3) cell lines. All three types of hybrid cells investigated, F1(DU145 x PC3), F1(JCA1 x PC3), and F1(JCA1 x ALVA31), were found to be resistant to Fas- and TNF-alpha-mediated apoptosis at the same level as the corresponding parental resistant cell lines. These results indicate that resistance to Fas- and TNF-alpha-mediated apoptosis dominates over sensitivity in cell hybrids and suggest that resistance may be regulated by an apoptosis suppressor factor or factors acting in resistant but not in sensitive cells.
Rokhlin
O W
OW
Department of Pathology, University of Iowa, Iowa City 52242, USA.
Hostager
B S
BS
Bishop
G A
GA
Sidorenko
S P
SP
Glover
R A
RA
Gudkov
A V
AV
Cohen
M B
MB
D017209:Apoptosis; D002277:Carcinoma / Q000473:pathology*; D002455:Cell Division; D053938:DNA Fragmentation; D005799:Genes, Dominant; D006801:Humans; D006822:Hybrid Cells; D016130:Immunophenotyping; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D019014:fas Receptor / Q000502:physiology*
D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor