title: Caprine arthritis encephalitis virus dysregulates the expression of cytokines in macrophages.
abstract: Caprine arthritis encephalitis virus (CAEV) is a lentivirus of goats that leads to chronic mononuclear infiltration of various tissues, in particular, the radiocarpal joints. Cells of the monocyte/macrophage lineage are the major host cells of CAEV in vivo. We have shown that infection of cultured goat macrophages with CAEV results in an alteration of cytokine expression in vitro. Constitutive expression of interleukin 8 (IL-8) and monocyte chemoattractant protein 1 (MCP-1) was increased in infected macrophages, whereas transforming growth factor beta1 (TGF-beta1) mRNA was down-regulated. When macrophages were infected with a CAEV clone lacking the trans-acting nuclear regulatory gene tat, IL-8 and MCP-1 were also increased. No significant differences from cells infected with the wild-type clone were observed, suggesting that Tat is not required for the increased expression of IL-8 and MCP-1 in infected macrophages. Furthermore, infection with CAEV led to an altered pattern of cytokine expression in response to lipopolysaccharide (LPS), heat-killed Listeria monocytogenes plus gamma interferon, or fixed cells of Staphylococcus aureus Cowan I. In infected macrophages, tumor necrosis factor alpha, IL-1beta, IL-6, and IL-12 p40 mRNA expression was reduced in response to all stimuli tested whereas changes in expression of granulocyte-macrophage colony-stimulating factor depended on the stimulating agent. Electrophoretic mobility shift assays demonstrated that, in contrast to effects of human immunodeficiency virus infection of macrophages, CAEV infection had no effect on the level of constitutive nuclear factor-kappaB (NF-kappaB) activity or on the level of LPS-stimulated NF-kappaB activity, suggesting that NF-kappaB is not involved in altered regulation of cytokine expression in CAEV-infected cells. In contrast, activator protein 1 (AP-1) binding activity was decreased in infected macrophages. These data show that CAEV infection may result in a dysregulation of expression of cytokines in macrophages. This finding suggests that CAEV may modulate the accessory functions of infected macrophages and the antiviral immune response in vivo.
authors.affiliation: Institute of Veterinary Virology, University of Bern, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Lechner
authors.forename: J
authors.initials: J
authors.lastname: Machado
authors.forename: G
authors.initials: G
authors.lastname: Bertoni
authors.forename: H F
authors.initials: HF
authors.lastname: Seow
authors.forename: D A
authors.initials: DA
authors.lastname: Dobbelaere
authors.forename: E
authors.initials: E
authors.lastname: Peterhans
mesh_terms: D000818:Animals; D017209:Apoptosis; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000276:immunology*; D015696:Gene Products, tat / Q000378:metabolism; D015756:Genes, tat; D006041:Goats; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006678:HIV / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016209:Interleukin-8 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D013211:Staphylococcus aureus / Q000276:immunology; D014158:Transcription, Genetic / Q000187:drug effects; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D018932:Chemokine CCL2; D016207:Cytokines; D015696:Gene Products, tat; D016209:Interleukin-8; D007378:Interleukins; D008070:Lipopolysaccharides; D054322:tat Gene Products, Human Immunodeficiency Virus; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor