Desulfovibrio inopinatus, sp. nov., a new sulfate-reducing bacterium that degrades hydroxyhydroquinone.
A new sulfate-reducing bacterium was isolated from marine sediment with hydroxyhydroquinone (1,2,4-trihydroxybenzene) as the sole electron and carbon source. Strain HHQ 20 grew slowly with doubling times of > 20 h and oxidized hydroxyhydroquinone, lactate, pyruvate, ethanol, fructose, and ribose incompletely to acetate and carbon dioxide, with concomitant reduction of sulfate to sulfide. Cells were large, vibrio-shaped, and gram-negative with a G+C content of 49.7 mol%, and contained desulfoviridin. Based on analysis of the 16S rRNA sequence, strain HHQ 20 was found to be related to the genus Desulfovibrio but formed a separate line, thus justifying the establishment of a new species within this genus. Hydroxyhydroquinone was the only aromatic compound utilized among numerous hydroxybenzoates, hydroxybenzenes, methoxybenzoates, and methoxybenzenes tested, suggesting that phloroglucinol and resorcinol are not degradation intermediates. Cell-free extracts of strain HHQ 20 did not contain pyrogallol-phloroglucinol transhydroxylase activity. First experiments indicated that this strain uses a new reductive pathway for anaerobic hydroxyhydroquinone degradation.
Reichenbecher
W
W
Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-78434 Konstanz, Germany.
Schink
B
B
D000085:Acetates / Q000378:metabolism; D000693:Anaerobiosis; D000840:Anisoles / Q000378:metabolism; D001482:Base Composition; D002245:Carbon Dioxide / Q000378:metabolism; D003901:Desulfovibrio / Q000145:classification* / Q000254:growth & development / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D050885:Hydrogensulfite Reductase; D006873:Hydroquinones / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000302:isolation & purification; D019800:Phenol / Q000378:metabolism; D010802:Phylogeny; D019289:Pyruvic Acid / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012266:Ribose / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism; D014871:Water Microbiology
D000085:Acetates; D000840:Anisoles; D006873:Hydroquinones; D062385:Hydroxybenzoates; D007773:Lactates; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D013440:Sulfides; D002245:Carbon Dioxide; C013038:hydroxyhydroquinone; D005632:Fructose; D019800:Phenol; D000431:Ethanol; D012266:Ribose; D019289:Pyruvic Acid; C060998:anisole; D050862:Oxidoreductases Acting on Sulfur Group Donors; D050885:Hydrogensulfite Reductase