Structure of IL-13 receptor: analysis of subunit composition in cancer and immune cells.
The structure of IL-13 receptor (IL-13R) is currently under investigation. Recently, two different human IL-13R chains, termed here IL-13R alpha and -alpha' have been cloned. We have examined various cancer and normal cell lines for the presence of mRNA for IL-13R alpha and alpha, as well as IL-4R p140 (termed beta chain) and IL-2R gamma c chains. In renal cell carcinoma, glioblastoma and ovarian carcinoma (IGROV-1) cell lines, both IL-13R alpha and alpha chains were expressed (type I IL-13R). In epidermoid, colon, ovarian adenocarcinoma (PA-1) and normal mouse fibroblast (COS7) cell lines, only IL-13R alpha' was expressed (type II IL-13R). In hematopoietic TF-1 and EBV-immortalized normal B cell lines only IL-13R alpha' but not alpha chain was expressed along with gamma c (type III or type IV IL-13R). IL-13R alpha' chain was faintly detected in human T cells. All cells expressed the IL-4Rp140 beta chain. These data provide a direct support for our model of IL-13R which consists of three different forms composed of different subunits.
Murata
T
T
Laboratory of Molecular Tumor Biology, Food and Drug Administration, Bethesda, Maryland 20892, USA.
Obiri
N I
NI
Debinski
W
W
Puri
R K
RK
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D019556:COS Cells; D002277:Carcinoma; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003110:Colonic Neoplasms; D005260:Female; D005909:Glioblastoma; D004854:Herpesvirus 4, Human; D006801:Humans; D018793:Interleukin-13 / Q000378:metabolism*; D053661:Interleukin-13 Receptor alpha1 Subunit; D007680:Kidney Neoplasms; D007951:Leukemia, Myeloid; D010051:Ovarian Neoplasms; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000737:chemistry*; D053660:Receptors, Interleukin-13; D014407:Tumor Cells, Cultured
C508181:IL13RA1 protein, human; C508183:Il13ra1 protein, mouse; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D018123:Receptors, Interleukin; D053660:Receptors, Interleukin-13