Incorporation of 1-deoxy-D-xylulose into isoprene and phytol by higher plants and algae.
In further substantiating the novel mevalonate-independent pathway for isoprenoid biosynthesis, which generates isopentenyl diphosphate (IPP) via 1-deoxy-D-xylulose-5-phosphate, labeling experiments with 1-[2H(1)]deoxy-D-xylulose were performed with various higher plants and algae: efficient incorporation was observed into isoprene emitted by Populus, Chelidonium, and Salix, into the phytol moiety of chlorophylls in a red alga (Cyanidium), in two green algae (Scenedesmus, Chlamydomonas), and a higher plant (Lemna). By contrast, 13C-mevalonate applied was incorporated into isoprene and phytol to a much lower extent or not at all. This demonstrates that this '1-deoxy-D-xylulose-5-phosphate pathway' for biosynthesis of plastidic isoprenoids is widely distributed in photosynthetic organisms.
Schwender
J
J
Botanisches Institut, Universität Karlsruhe, Germany.
Zeidler
J
J
Gröner
R
R
Müller
C
C
Focke
M
M
Braun
S
S
Lichtenthaler
F W
FW
Lichtenthaler
H K
HK
D002070:Butadienes / Q000378:metabolism*; D002247:Carbon Isotopes; D000460:Chlorophyta / Q000378:metabolism; D003903:Deuterium; D056890:Eukaryota / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism; D045782:Hemiterpenes; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000378:metabolism*; D008798:Mevalonic Acid / Q000378:metabolism; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000378:metabolism; D010420:Pentanes; D010836:Phytol / Q000378:metabolism*; D000461:Rhodophyta / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis; D014996:Xylulose / Q000031:analogs & derivatives* / Q000378:metabolism
D002070:Butadienes; D002247:Carbon Isotopes; D006027:Glycosides; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D010420:Pentanes; D013261:Sterols; C005059:isoprene; D010836:Phytol; C004809:isopentenyl pyrophosphate; D014996:Xylulose; C029724:1-deoxy-2-pentulose; D003903:Deuterium; D008798:Mevalonic Acid