Rice lipid transfer protein (LTP) genes belong to a complex multigene family and are differentially regulated.
Several cDNA clones encoding three different lipid transfer proteins (LTPs) have been isolated from rice (Oryza sativa L.) in order to analyse the complexity, the evolution and the expression of the LTP gene family. The mature proteins deduced from three clones exhibited a molecular mass of 9 kDa, in agreement with the molecular mass of other LTPs from plants. The clones were shown to be homologous in the coding region, while the 3' non-coding regions diverged strongly between the clones. The occurrence of at least three small multigene families encoding these proteins in rice was confirmed by Southern blot analysis. When compared with each other and with LTPs from other plants, the cluster including rice LTPs and other cereal LTPs indicated that these genes duplicated rather recently and independently in the different plant phyla. The expression pattern of each gene family was also investigated. Northern blot experiments demonstrated that they are differentially regulated in the different tissues analysed. Components such as salt, salicylic acid and abscisic acid were shown to modulate Ltp gene expression, depending on tissues and gene classes, suggesting a complex regulation of these genes.
Vignols
F
F
Laboratoire de Physiologie Cellulaire et Moléculaire, URA CNRS 1180, Université de Paris VI, France.
Wigger
M
M
García-Garrido
J M
JM
Grellet
F
F
Kader
J C
JC
Delseny
M
M
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
D052179:Antigens, Plant; D002352:Carrier Proteins; D018744:DNA, Plant; D010940:Plant Proteins; D012459:Salicylates; C511920:lipid transfer proteins, plant; D012965:Sodium Chloride; D000040:Abscisic Acid; D020156:Salicylic Acid