Regulation and expression of D-1, but not D-5, dopamine receptors in human SK-N-MC neuroblastoma cells.
Human SK-N-MC neuroblastoma cells endogenously express functional D-1-like dopamine receptors. The recent identification of at least two distinct type of D-1-like receptors, D-1 and D-5, lead us to investigate the precise molecular identify of the receptors expressed in these cells. By using specific primer sets and amplification of the mRNA by RT-PCR, we show that only D-1, but not D-5, dopamine receptors are expressed in these cells. Treatment of cells with 100 microM dopamine initially caused an upregulation in D-1 mRNA expression, followed by attenuation of the message compared to control, untreated cells. The D-1 receptors in SK-N-MC cells, whose expression is controlled by dopamine in a bimodal manner, may be important in understanding how these receptors are regulated at the molecular level.
Sidhu
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Department of Pediatrics, Georgetown University Medical Center, Georgetown University, Washington D.C. 2007, USA. sidhua@odrge.odr.georgetown.edu
D000262:Adenylyl Cyclases / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009447:Neuroblastoma / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017447:Receptors, Dopamine D1 / Q000096:biosynthesis* / Q000235:genetics; D050641:Receptors, Dopamine D5; D014407:Tumor Cells, Cultured
C493605:DRD5 protein, human; D012333:RNA, Messenger; D017447:Receptors, Dopamine D1; D050641:Receptors, Dopamine D5; D000262:Adenylyl Cyclases; D004298:Dopamine