title: Neuroendocrine cells in the prostate of the rat, guinea pig, cat, and dog.
abstract: BACKGROUND
  The neuroendocrine (NE) cells in the human prostate gland probably have a local regulatory role in both prostatic growth and differentiation as well as in the exocrine secretory process. Moreover, NE cells may be involved in the pathogenesis of both prostatic cancer and hyperplasia. To enhance the knowledge of the physiological and pathophysiological role of NE cells in the prostate gland, we wanted to establish an experimental animal model.
  
  
  METHODS
  All lobes of the prostatic complex of rats with different serum levels of testosterone, as well as the prostate of the guinea pig, cat, and dog, were studied. Prostatic tissue fixed in different fixatives was studied with regard to NE cells by using cytochemical and immunohistochemical staining techniques, as well as Northern blotting and reverse transcriptase polymerase chain reaction (RT-PCR) for detection of rat chromogranin A (CgA) mRNA.
  
  
  RESULTS
  The present study indicates the absence of NE cells in the rat prostatic complex. No expression of CgA RNA was detectable either by Northern blotting or by RT-PCR. Only a few argyrophil cells in the prostatic complex of guinea pig were detected in tissue fixed in Bouin's solution.
  
  
  CONCLUSIONS
  Rat, guinea pig, cat, and dog are not suitable animals in physiological studies of NE cells in the prostate gland.
authors.affiliation: Department of Physiology and Biomedical Engineering, Norwegian University of Technology and Science, Trondheim, Norway.
authors.forename: A
authors.initials: A
authors.lastname: Angelsen
authors.forename: R
authors.initials: R
authors.lastname: Mecsei
authors.forename: A K
authors.initials: AK
authors.lastname: Sandvik
authors.forename: H L
authors.initials: HL
authors.lastname: Waldum
mesh_terms: D000818:Animals; D002415:Cats / Q000033:anatomy & histology*; D004285:Dogs / Q000033:anatomy & histology*; D006168:Guinea Pigs / Q000033:anatomy & histology*; D007150:Immunohistochemistry; D008297:Male; D009490:Neurosecretory Systems / Q000166:cytology*; D009919:Orchiectomy; D011467:Prostate / Q000166:cytology*; D051381:Rats / Q000033:anatomy & histology*; D012835:Silver Nitrate; D016622:Silver Staining; D013739:Testosterone / Q000097:blood
chemical_list: C083000:Grimelius stain; D013739:Testosterone; D012835:Silver Nitrate