Molecular cloning of a cDNA encoding the amphibian Pleurodeles waltl 70-kDa heat-shock cognate protein.
We isolated and characterized a cDNA coding for heat-shock protein 70 of the amphibian Pleurodeles waltl. This 2212-bp sequence exhibited one open reading frame of 645 amino acids. The predicted amino acid sequence exhibited the three conserved elements of the HSC/HSP70 protein family. Comparison of nucleotide and amino acid sequences between this gene and other hsc/hsp-like genes revealed a high identity with the cognate form HSC70. By in vitro translation, this gene encoded a 70-kDa protein which was different than the inducible Pleurodeles waltl HSP70 protein. This translated protein was recognized by Pleurodeles waltl N1 anti-HSC/HSP70 antibody. Heat-inducibility tests showed that this gene was constitutively expressed during oogenesis and embryogenesis, and its expression was not increased after a heat-shock. These results led us to conclude that we recovered a Pleurodeles waltl cognate hsc70 gene.
Delelis-Fanien
C
C
UA 1135, Université P-et-M-Curie, CNRS, Laboratoire de Biologie Moléculaire et Cellulaire du Développement, Paris, France.
Penrad-Mobayed
M
M
Angelier
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D019143:Evolution, Molecular; D005260:Female; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D010999:Pleurodeles / Q000196:embryology / Q000235:genetics*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013623:Tail / Q000196:embryology
D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins