Quantitative determination of endogenous nitric oxide in the mouse skin in vivo by microdialysis.
We have developed a subcutaneous microdialysis system for the determination of nitric oxide (NO) concentration in the skin. The skin was microdialyzed using a degassed solution containing 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide and the perfusate was reacted on-line with Griess' reagent. This method could reveal NO production following intradermal injection of bradykinin (10-100 nmol/site) in mice. The increase in cutaneous NO after bradykinin (100 nmol/site) was dose dependently suppressed by the NO synthase inhibitor. NG-nitro-L-arginine methyl ester, and the bradykinin B, receptor antagonist, D-Arg-[Hyp3, Thi5.8, D-Phe7]-bradykinin. This system may be useful for pharmacological and physiological experiments on the role of NO in the skin.
Andoh
T
T
Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Kuraishi
Y
Y
D000818:Animals; D001565:Benzoates; D001920:Bradykinin / Q000031:analogs & derivatives / Q000494:pharmacology; D065168:Bradykinin Receptor Antagonists; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007093:Imidazoles; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D017551:Microdialysis; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000032:analysis* / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D043782:Receptor, Bradykinin B2; D012867:Skin / Q000737:chemistry* / Q000378:metabolism
D001565:Benzoates; D065168:Bradykinin Receptor Antagonists; D004791:Enzyme Inhibitors; D007093:Imidazoles; D043782:Receptor, Bradykinin B2; C053748:B 4162; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D001920:Bradykinin; D019331:NG-Nitroarginine Methyl Ester