Development of a Haemophilus two-dimensional protein database.
Members of the Haemophilus genus are responsible for various human infections including respiratory infections and meningitis. The complete nucleotide sequence of the Rd strain of Haemophilus influenzae has been reported and represents a valuable resource to investigate gene expression within this bacterial group. We described previously the application of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) to characterise the proteins of Haemophilus influenzae (Cash et al., Electrophoresis 1995, 16, 135-148). We have extended these data with comparative studies of the proteins from other members of the Haemophilus genus (specifically H. parainfluenzae, H. haemolyticus and H. parahaemolyticus) to identify homologous proteins and, by extension, the genes encoding them, among these bacteria. The proteins extracted from each of these bacterial isolates were compared by coelectrophoresis to the 2-D protein profile of the reference nontypable strain of H. influenzae (HI-64443) used as the basis for the 2-D protein database. A composite reference 2-D protein profile of HI-64443 was derived from three independent analyses of the soluble bacterial proteins. Between 21% and 37% of the HI-64443 proteins from the reference 2-D protein profile comigrated with proteins in the other isolates from the Haemophilus genus. This compared with 62% and 64% comigration when HI-64443 was compared with the Eagan and Rd strains of H. influenzae, respectively. The 2-D protein profile of the Rd strain of H. influenzae was compared to that of HI-64443 by coelectrophoresis; 64% of the proteins detected for the Rd strain comigrated with proteins found for HI-64443 when analysed in parallel. The capacity of 2-D PAGE to investigate global interactions of gene expression was applied to the analysis of superoxide dismutase (SOD) expression in H. influenzae strain Eagan. A "knock-out" mutant in the sodA gene which encodes [Mn]-SOD was characterised with respect to protein synthesis compared to the parental isolate. From these analyses, the primary product of sodA was provisionally identified as a protein with a molecular mass of 25500 Da and an estimated pI of 6.55. Quantitative changes in the expression of two other proteins in the SOD mutant were detected by comparison with the parental isolate. These data are discussed in relation to the development of a 2-D protein database for H. influenzae and related bacteria to investigate genome homologies and gene expression.
Cash
P
P
Department of Medical Microbiology, University of Aberdeen, Foresterhill, UK. p.cash@abdn.ac.uk
Argo
E
E
Langford
P R
PR
Kroll
J S
JS
D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification*; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods* / Q000706:statistics & numerical data; D015870:Gene Expression; D016680:Genome, Bacterial; D006190:Haemophilus / Q000737:chemistry / Q000235:genetics; D006193:Haemophilus influenzae / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009154:Mutation; D010449:Peptide Mapping / Q000379:methods / Q000706:statistics & numerical data; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000235:genetics / Q000302:isolation & purification
D001426:Bacterial Proteins; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase