Isolation and characterization of Exodus-2, a novel C-C chemokine with a unique 37-amino acid carboxyl-terminal extension.
Chemokines are a group of small, homologous proteins that regulate leukocyte migration, hemopoiesis, and HIV-1 absorption. We report here the cloning and characterization of a novel murine and human C-C chemokine termed Exodus-2 for its similarity to Exodus-1/MIP-3alpha/LARC, and its chemotactic ability. This novel chemokine has a unique 36 or 37 (murine and human, respectively) amino acid carboxyl-terminal extension not seen in any other chemokine family member. Purified recombinant Exodus-2 was found to have two activities classically associated with chemokines: inhibiting hemopoiesis and stimulating chemotaxis. However, Exodus-2 also had unusual characteristics for C-C chemokines. It selectively stimulated the chemotaxis of T-lymphocytes and was preferentially expressed in lymph node tissue. The combination of these characteristics may be a functional correlate for the unique carboxyl-terminal structure of Exodus-2.
Hromas
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R
Department of Medicine, Indiana University Medical Center, Indianapolis 46202, USA. robertvhromas@iucc.iupui.edu
Kim
C H
CH
Klemsz
M
M
Krathwohl
M
M
Fife
K
K
Cooper
S
S
Schnizlein-Bick
C
C
Broxmeyer
H E
HE
D000595:Amino Acid Sequence; D000818:Animals; D054421:Chemokine CCL21; D018925:Chemokines / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D019742:Chemokines, CC; D002633:Chemotaxis / Q000187:drug effects; D003001:Cloning, Molecular; D006801:Humans; D007962:Leukocytes / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis
C515478:CCL21 protein, human; C515479:Ccl21c protein, mouse; D054421:Chemokine CCL21; D018925:Chemokines; D019742:Chemokines, CC