title: Ancient DNA is thirteen years old.
abstract: The first successful recovery of ancient DNA, from quagga and human mummies inspired significant enough interest to open an entire field of research. Efforts from many research groups, often in a hunt for the oldest sequences, showed that ancient DNA was a poor substrate for the enzymes used in molecular biology; it is present in tiny amounts, hard to purify, and frequently damaged. These obstacles have been partially overcome by the use of drastic laboratory precautions and by the introduction of polymerase chain reaction and phylogenetic studies. Ancient DNA analysis now finds applications in many research domains.
authors.affiliation: E.P.91 Structural and Genetic Information IBSM-CNRS, Marseille, France.
authors.forename: S
authors.initials: S
authors.lastname: Audic
authors.forename: E
authors.initials: E
authors.lastname: Béraud-Colomb
mesh_terms: D000818:Animals; D001106:Archaeology; D003141:Communicable Diseases / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D030342:Genetic Diseases, Inborn / Q000235:genetics; D006801:Humans; D010163:Paleontology; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; D004247:DNA