Severe digestive pathology associated with chronic Chagas' disease in Ecuador: report of two cases.
DNA extracted from peripheral blood of two Ecuadorian patients showing severe digestive pathology was amplified by the polymerase chain reaction using a Trypanosoma cruzi specific oligonucleotide primers derived from the primary sequence of a cDNA encoding for a 24 kDa excretory/secretory protein. The positive PCR results together with the clinical findings confirmed that both patients had a digestive pathology due to Chagas' disease. This pathology could be more frequent than previously described in the chagasic endemic regions of Andean countries.
Guevara
A G
AG
Department of Clinical Investigations, Hospital Vozandes, Quito, Ecuador.
Eras
J W
JW
Recalde
M
M
Vinueza
L
L
Cooper
P J
PJ
Ouaissi
A
A
Guderian
R H
RH
D000368:Aged; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D014355:Chagas Disease / Q000150:complications* / Q000175:diagnosis; D002908:Chronic Disease; D016054:DNA, Protozoan / Q000097:blood; D004066:Digestive System Diseases / Q000175:diagnosis / Q000209:etiology*; D004484:Ecuador; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014349:Trypanosoma cruzi / Q000235:genetics / Q000276:immunology
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan