Rapid identification of Newcastle disease virus vaccine strains LaSota and B-1 by restriction site analysis of their matrix gene.
A region constituting 88% of the matrix gene of Newcastle disease virus vaccine strains LaSota and B-1 was amplified by reverse transcription-polymerase chain reaction. Amplified products of LaSota and B-1 strains derived from vaccine serials of different companies were digested with restriction enzymes MboI and HinfI. Strain characteristic cleavage site maps were obtained that allowed for a reliable and rapid differentiation between strains LaSota and B-1.
Wehmann
E
E
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary.
Herczeg
J
J
Ballagi-Pordány
A
A
Lomniczi
B
B
D000818:Animals; D002642:Chick Embryo; D005814:Genes, Viral; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D015183:Restriction Mapping; D014765:Viral Vaccines / Q000276:immunology*
D014765:Viral Vaccines