[The functional properties of the cellular structures of cultured Treponema pallidum].
New data on the functional properties of the cell structures of cultured T. pallidum were obtained, which permitted the construction of new more effective preparations for the diagnostics of syphilis. T. pallidum cell walls were used as antigen in the complement fixation test (CFT) and the development of a new enzyme immunoassay system for the determination of IgM and IgG antibodies to T. pallidum with the visual evaluation of results, while T. pallidum cytoplasm was used as sorbent in the immunofluorescence test (IFT-abs) for the serodiagnosis of syphilis. The immunization of rabbits with T. pallidum cell walls increased the titers of positive control sera used in CFT for diagnosing syphilis.
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D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D002473:Cell Wall / Q000276:immunology; D003470:Culture Media; D003593:Cytoplasm / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D008297:Male; D011817:Rabbits; D013587:Syphilis / Q000175:diagnosis; D013588:Syphilis Serodiagnosis / Q000379:methods; D014210:Treponema pallidum / Q000166:cytology / Q000276:immunology / Q000502:physiology*
D000942:Antigens, Bacterial; D003470:Culture Media; D007106:Immune Sera