[Study of incorporation of highly-cleaved H7 hemagglutinin, expressed by a recombinant vector in virions of superinfecting influenza viruses].
CV-1 cells were preinfected with H7-SV40 recombinant vector and superinfected with influenza viruses A/USSR/90/77 (H1N1), A/Duck/Czechoslovakia/56 (H4N6), or B/Hongkong/73. A fraction of the yields of influenza A viruses was infectious without trypsin treatment due to the incorporation of cleaved H7 hemagglutinin into virions. The specificity of the effect was confirmed by neutralization of the infectivity with anti-H7 serum. At a low multiplicity of infection the incorporation of H7 hemagglutinin into virions allowed the virus to replicate without trypsin in a multi-cycle manner. The fraction of the infectious virus and the kinetics of the multi-cycle infection in H7-expressing cells were similar in the cells superinfected with A/USSR/90/77 and with A/Duck/Czechoslovakia/56 viruses, thus demonstrating a low specificity of interaction between H7 hemagglutinin and heterologous M2 proteins. The superinfection of H7-expressing cells with influenza B/Hongkong/73 virus did not produce infectious phenotypically mixed virus, which may be regarded as an indication of inability of influenza B virus NB protein to replace functionally the influenza A virus M2 protein in its interaction with hemagglutinin.
Grodnitskaia
N A
NA
Rudneva
I A
IA
Makarova
N V
NV
Kaverin
N V
NV
D006389:Hemagglutinins, Viral / Q000276:immunology / Q000378:metabolism*; D007106:Immune Sera; D009980:Influenza A virus / Q000378:metabolism* / Q000472:pathogenicity / Q000502:physiology; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D015163:Superinfection; D014771:Virion / Q000378:metabolism*; D014779:Virus Replication
D006389:Hemagglutinins, Viral; D007106:Immune Sera; D011994:Recombinant Proteins