title: Use of two reverse transcriptases eliminates false-positive results in differential display.
abstract: The selection of false-positive clones in differential display PCR (DDRT-PCR) represents a formidable drawback to this otherwise powerful technique of detecting subtle differences between cell types. DDRT-PCR performed with cDNAs generated by two different reverse transcriptases from the same RNA doubles the total number of reactions; nevertheless, false-positive clones arising from small differences between RNA preparations are easily distinguished from differentially expressed transcripts.
authors.affiliation: Columbia University College of Physicians and Surgeons New York, NY, USA.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Sung
authors.forename: R B
authors.initials: RB
authors.lastname: Denman
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D019556:COS Cells; D018076:DNA, Complementary / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005189:False Positive Reactions; D005822:Genetic Vectors; D008668:Metallothionein / Q000235:genetics; D008979:Moloney murine leukemia virus / Q000201:enzymology; D016133:Polymerase Chain Reaction / Q000379:methods*; D016337:RNA, Catalytic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D016914:Ribonuclease H
chemical_list: D016564:Amyloid beta-Protein Precursor; D018076:DNA, Complementary; D016337:RNA, Catalytic; D012333:RNA, Messenger; D008668:Metallothionein; D012194:RNA-Directed DNA Polymerase; D016914:Ribonuclease H