title: Two alpha(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (S) and Escherichia coli F18 receptor (ECF18R) loci.
abstract: The Escherichia coli F18 receptor locus (ECF18R) has been genetically mapped to the halothane linkage group on porcine Chromosome (Chr) 6. In an attempt to obtain candidate genes for this locus, we isolated 5 cosmids containing the alpha (1,2)fucosyltransferase genes FUT1, FUT2, and the pseudogene FUT2P from a porcine genomic library. Mapping by fluorescence in situ hybridization placed all these clones in band q11 of porcine Chr 6 (SSC6q11). Sequence analysis of the cosmids resulted in the characterization of an open reading frame (ORF), 1098 bp in length, that is 82.3% identical to the human FUT1 sequence; a second ORF, 1023 bp in length, 85% identical to the human FUT2 sequence; and a third FUT-like sequence thought to be a pseudogene. The FUT1 and FUT2 loci therefore seem to be the porcine equivalents of the human blood group H and Secretor loci. Direct sequencing of the two ORFs in swine being either susceptible or resistant to adhesion and colonization by F18 fimbriated Escherichia coli (ECF18) revealed two polymorphisms at bp 307 (M307) and bp 857 (M857) of the FUT1 ORF. Analysis of these mutations in 34 Swiss Landrace families with 221 progeny showed close linkage with the locus controlling resistance and susceptibility to E. coli F18 adhesion and colonization in the small intestine (ECF18R), and with the locus of the blood group inhibitor S. A high linkage disequilibrium of M307-ECF18R in Large White pigs makes the M307 mutation a good marker for marker-assisted selection of E. coli F18 adhesion-resistant animals in this breed. Whether the FUT1 or possibly the FUT2 gene products are involved in the synthesis of carbohydrate structures responsible for bacterial adhesion remains to be determined.
authors.affiliation: Institute of Animal Science, Swiss Federal Institute of Technology, ETH-Zentrum, Zurich, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Meijerink
authors.forename: R
authors.initials: R
authors.lastname: Fries
authors.forename: P
authors.initials: P
authors.lastname: Vögeli
authors.forename: J
authors.initials: J
authors.lastname: Masabanda
authors.forename: G
authors.initials: G
authors.lastname: Wigger
authors.forename: C
authors.initials: C
authors.lastname: Stricker
authors.forename: S
authors.initials: S
authors.lastname: Neuenschwander
authors.forename: H U
authors.initials: HU
authors.lastname: Bertschinger
authors.forename: G
authors.initials: G
authors.lastname: Stranzinger
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D003360:Cosmids; D005647:Fucosyltransferases / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015810:Linkage Disequilibrium; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D011110:Polymorphism, Genetic; D011544:Pseudogenes / Q000235:genetics*; D011971:Receptors, Immunologic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D018830:Adhesins, Escherichia coli; D005647:Fucosyltransferases; D005819:Genetic Markers; D011971:Receptors, Immunologic; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C047105:bacterial adhesin receptor