title: Ammonium acetate protocol for the preparation of plasmid DNA suitable for mammalian cell transfections.
authors.affiliation: Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Saporito-Irwin
authors.forename: R T
authors.initials: RT
authors.lastname: Geist
authors.forename: D H
authors.initials: DH
authors.lastname: Gutmann
mesh_terms: D000085:Acetates; D000818:Animals; D000838:Anions; D019556:COS Cells; D002498:Centrifugation; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D025581:Neurofibromin 2; D010446:Peptide Fragments / Q000032:analysis; D010957:Plasmids; D014162:Transfection; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D000085:Acetates; D000838:Anions; D004396:Coloring Agents; D008565:Membrane Proteins; D025581:Neurofibromin 2; D010446:Peptide Fragments; D004247:DNA; D001616:beta-Galactosidase; D004996:Ethidium; C018824:ammonium acetate