Effects of cytokine-induced neutrophil chemoattractants (CINCs) on shape change, adhesiveness and phagocytosis of rat neutrophils.
Rat cytokine-induced neutrophil chemoattractants (CINC)-1, -2 alpha, -2 beta and -3 belong to alpha-chemokines and are known to be potent chemoattractants for rat neutrophils in vivo and in vitro. In this study, the abilities of CINCs to affect several functions of rat neutrophils are estimated. All CINCs at a concentration of 10(-8) M induced the polarization of rat neutrophils. The adhesion of each CINC-treated neutrophil to fibrinogen significantly increased, reaching a maximal adhesion at 10(-8) M of each CINC. The CINCs enhanced the phagocytosis of heat-killed yeast cells by neutrophils in a dose-dependent manner. In contrast, CINCs did not induce the production of reactive nitrogen intermediates. The results suggest that CINCs play a significant role in neutrophil infiltration and phagocytosis at inflammatory sites in rats.
al-Mokdad
M
M
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Shibata
F
F
Nakagawa
H
H
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D016764:Cell Polarity; D002630:Chemotactic Factors / Q000378:metabolism*; D016207:Cytokines / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry; D066298:In Vitro Techniques; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D009573:Nitrites / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D051381:Rats
D002630:Chemotactic Factors; D016207:Cytokines; D009573:Nitrites; D005340:Fibrinogen