title: Initiation and bidirectional propagation of chromatin assembly from a target site for nucleotide excision repair.
abstract: To restore full genomic integrity in a eukaryotic cell, DNA repair processes have to be coordinated with the resetting of nucleosomal organization. We have established a cell-free system using Drosophila embryo extracts to investigate the mechanism linking de novo nucleosome formation to nucleotide excision repair (NER). Closed-circular DNA containing a uniquely placed cisplatin-DNA adduct was used to follow chromatin assembly specifically from a site of NER. Nucleosome formation was initiated from a target site for NER. The assembly of nucleosomes propagated bidirectionally, creating a regular nucleosomal array extending beyond the initiation site. Furthermore, this chromatin assembly was still effective when the repair synthesis step in the NER process was inhibited.
authors.affiliation: Institut Curie/Research section UMR 144 du CNRS, 26 rue d'Ulm 75231 Paris cedex 05, France.
authors.forename: Pierre-Henri L
authors.initials: PHL
authors.lastname: Gaillard
authors.forename: J G
authors.initials: JG
authors.lastname: Moggs
authors.forename: D M
authors.initials: DM
authors.lastname: Roche
authors.forename: J P
authors.initials: JP
authors.lastname: Quivy
authors.forename: P B
authors.initials: PB
authors.lastname: Becker
authors.forename: R D
authors.initials: RD
authors.lastname: Wood
authors.forename: G
authors.initials: G
authors.lastname: Almouzni
mesh_terms: D000818:Animals; D002474:Cell-Free System; D002843:Chromatin / Q000378:metabolism*; D002945:Cisplatin / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D018736:DNA Adducts / Q000378:metabolism; D004260:DNA Repair; D004270:DNA, Circular / Q000378:metabolism; D004330:Drosophila; D004625:Embryo, Nonmammalian; D006801:Humans; D008957:Models, Genetic; D014981:Xenopus
chemical_list: D002843:Chromatin; D018736:DNA Adducts; D004270:DNA, Circular; D004247:DNA; D002945:Cisplatin