title: Metacyclogenesis modulates the ability of Leishmania promastigotes to induce IL-12 production in human mononuclear cells.
abstract: Immunity against the intracellular protozoan Leishmania species is highly dependent on Th1 development. We have previously shown that IL-12 is a powerful and probably obligatory factor for Th1 cell generation and proliferation. We also observed that the experimental infection of C3H and BALB/c mice with Leishmania major is associated with IL-12 production in vivo. Now we demonstrate that metacyclic L. major promastigotes are poor inducers of IL-12 in vitro in fresh human PBMC and monocytes. In addition, we show that the ability of this pathogen to induce IL-12 and other cytokines is modulated by the metacyclogenic process, which had previously not been recognized. In contrast to the infective parasites (metacyclic promastigotes), the procyclic promastigotes collected at the logarithmic phase of the culture displayed a striking ability to induce IL-12, IFN-gamma, TNF-alpha, and IL-10. Despite this differential effect of procyclic and metacyclic parasites in terms of IL-12 induction, both stages were inhibitory for IL-12 production induced by Staphylococcus aureus. The ability of procyclic promastigotes and, to a much lesser extent, that of metacyclic promastigotes to induce IL-12 were enhanced by pretreatment of monocytes in a cytokine milieu containing IFN-gamma, IL-4, IL-13, or granulocyte-macrophage CSF or by neutralization of endogenous IL-10. Our results suggest the development of an evasion mechanism as the Leishmania promastigotes mature to infectious forms and the possibility of using Ags derived from procyclic promastigotes for immunization procedures.
authors.affiliation: Wistar Institute, Philadelphia, PA 19104, USA.
authors.forename: A
authors.initials: A
authors.lastname: Sartori
authors.forename: M A
authors.initials: MA
authors.lastname: Oliveira
authors.forename: P
authors.initials: P
authors.lastname: Scott
authors.forename: G
authors.initials: G
authors.lastname: Trinchieri
mesh_terms: D000818:Animals; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis*; D018320:Leishmania major / Q000254:growth & development / Q000276:immunology*; D051379:Mice; D009000:Monocytes / Q000276:immunology* / Q000469:parasitology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma