A novel aortic smooth muscle cell line obtained from p53 knock out mice expresses several differentiation characteristics.
Here we report that we could obtain a highly differentiated smooth muscle cell line by screening the expression of a-smooth muscle actin from p53 knook out mice aorta. This cell revealed extended bipolar shape and expressed h-caldesmon and calponin as well as a-smooth muscle actin as protein markers of differentiated smooth muscle. Further intracellular calcium increase was induced by application of noradrenaline in a dose dependent manner and calcium oscillation was also observed in a higher dose (100 microM). Appropriate application of 5-azacytidine enhanced these tendencies and induced slow contraction by endothelin-1 and phenylephrine.
Ohmi
K
K
Department of 1st Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
Masuda
T
T
Yamaguchi
H
H
Sakurai
T
T
Kudo
Y
Y
Katsuki
M
M
Nonomura
Y
Y
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D002469:Cell Separation; D002999:Clone Cells; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D009119:Muscle Contraction / Q000235:genetics; D009124:Muscle Proteins / Q000096:biosynthesis; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000235:genetics*
D009124:Muscle Proteins; D011942:Receptors, Adrenergic, alpha; D016159:Tumor Suppressor Protein p53; D009638:Norepinephrine