Molecular cloning of human GATA-6 DNA binding protein: high levels of expression in heart and gut.
Human GATA-6 has been cloned from foetal heart by a combination of PCR-based methods and cDNA library screening. The 3.8 kbp cDNA has a coding sequence of 1347 bp the 449 aa protein is virtually identical in the two zinc-finger binding domains to other human GATA sequences, but varies considerably in the amino and carboxy terminal regions. The sequence shows greatest similarity to GATA-6-like sequences from rat, mouse, chicken and Xenopus. Northern analysis and in situ hybridisation show that GATA-6 is expressed at high levels in human adult and foetal heart as well as in gut derivatives. It is postulated that GATA-6, in concert with GATA-4, plays a crucial role in the regulation of cardiac differentiation.
Huggon
I C
IC
Department of Molecular Medicine, Institute of Liver Studies, King's College School of Medicine and Dentistry, Camberwell, London, UK.
Davies
A
A
Gove
C
C
Moscoso
G
G
Moniz
C
C
Foss
Y
Y
Farzaneh
F
F
Towner
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D004064:Digestive System / Q000378:metabolism; D005333:Fetus / Q000378:metabolism; D050993:GATA6 Transcription Factor; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050993:GATA6 Transcription Factor; C494759:GATA6 protein, human; D014157:Transcription Factors