[The effect of bronchial inhalation provocation tests on levels of interleukin-8 in material from broncho-alveolar fluid of patients with atopic bronchial asthma].
Airway inflammation is a very important feature of asthma and occurs simultaneously with increased hyperreactivity. We have examined whether the local inflammation provoked by histamine and allergen challenge of patients with atopic bronchial asthma is associated with the appearance, in vivo interleukin-B (IL-8) in bronchoalveolar lavage fluid (BAL). Bronchoscopy and BAL for further IL-8 investigation were performed before and 24 h after challenge test with histamine (n = 11), grass pollen antigen (n = 8) and PBS (n = 5). ELISA test was used to measure IL-8 concentration (pg/ml) (kits from R&D, USA). There was observed increased level of IL-8 (p < 0.05) after histamine and allergen challenge test. This increased level of IL-8 was correlated with neutrophils in BAL (Kendall's correlation coefficient = +0.5). We conclude that IL-8 may participate in creation of bronchial hyperreactivity in atopic bronchial asthma.
Jahnz-Rózyk
K
K
Kliniki Chorób Wewnetrznych i Pneumonologii Instytutu Medycyny Wewnetrznej.
Pirozyńska
E
E
Pojda
Z
Z
D000328:Adult; D001249:Asthma / Q000209:etiology*; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis; D008297:Male; D012129:Respiratory Function Tests; D012130:Respiratory Hypersensitivity / Q000150:complications / Q000175:diagnosis* / Q000503:physiopathology
D016209:Interleukin-8