Toward clinical trials of DNA vaccines against malaria.
In mid 1997 the first malaria DNA vaccine will enter clinical trials. This single gene DNA vaccine encoding the Plasmodium falciparum circumsporozoite protein (PfCSP) will be studied for safety and immunogenicity. If these criteria are met, a multi-gene DNA vaccine designed to induce protective CD8+ T cell responses against P. falciparum infected hepatocytes will be subsequently assessed for safety, immunogenicity and capacity to protect immunized volunteers against experimental challenge with P. falciparum sporozoites. Our perspectives on malaria vaccine development in general, and on a multi-gene DNA vaccine in particular, have been recently reviewed. Herein, we review the rationale and experimental foundation for the anticipated P. falciparum DNA vaccine trials.
Hoffman
S L
SL
Malaria Program, Naval Medical Research Institute, Bethesda, Maryland 20852, USA. hoffmans@nmripo.nmri.nnmc.navy.mil
Doolan
D L
DL
Sedegah
M
M
Wang
R
R
Scheller
L F
LF
Kumar
A
A
Weiss
W R
WR
Le
T P
TP
Klinman
D M
DM
Hobart
P
P
Norman
J A
JA
Hedstrom
R C
RC
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002986:Clinical Trials as Topic; D007110:Immunity, Active; D008107:Liver Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D008253:Macaca mulatta; D008288:Malaria / Q000517:prevention & control*; D051379:Mice; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000627:therapeutic use*
D000953:Antigens, Protozoan; D019444:Vaccines, DNA