title: Primary structure of seven sulfated oligosaccharide-alditols released by reductive beta-elimination from oviducal mucins of Rana temporaria. Characterization of the sequence HSO3 (3) GlcA (beta1--3)Gal.
abstract: The mucins isolated from Rana temporaria egg jelly coats were found to be composed of Gal, Fuc, GlcNac, GalNAc and GlcA acid. The primary structure of seven sulfated oligosaccharide-alditols was obtained by 1D/2D NMR analyses (1H-13C). The results show the presence of the sulfate monosaccharides. HSO3(3)Gal, HSO(6)GlcNAc and HSO3(3)GlcA. The sequence HSO3(3)GlcA (beta1-3)Gal, which constitutes the major determinant of the HNK-1 oncofoetal epitope, was characterized.
authors.affiliation: Department de Biochimie, Université de Bucarest, Romania.
authors.forename: D
authors.initials: D
authors.lastname: Florea
authors.forename: E
authors.initials: E
authors.lastname: Maes
authors.forename: G
authors.initials: G
authors.lastname: Strecker
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000737:chemistry; D005697:Galactosides / Q000737:chemistry; D005965:Glucuronates / Q000737:chemistry; D020723:Glucuronic Acid; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011896:Rana temporaria; D017421:Sequence Analysis; D013402:Sugar Alcohols / Q000737:chemistry*; D013463:Sulfuric Acid Esters / Q000737:chemistry*; D015044:Zona Pellucida / Q000737:chemistry*
chemical_list: D004187:Disaccharides; D005697:Galactosides; D005965:Glucuronates; D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols; D013463:Sulfuric Acid Esters; D020723:Glucuronic Acid