Enzymatic synthesis of oligosaccharides containing Gal beta-->4Gal disaccharide at the non-reducing end using beta-galactanase from Penicillium citrinum.
The transglycosylation reaction was done with a beta-galactanase from Penicillium citrinum. The regioselectivity in the transglycosylation reaction was studied using soy bean arabinogalactan as a donor and mono- or disaccharide derivatives containing beta-galactosyl residue as acceptors. We also synthesized oligosaccharides containing Gal beta 1-->4Gal sequence such as Gal beta 1-->4Gal beta1-->4Glc, Gal beta 1-->4Gal beta 1-->3GlcNac, Gal beta 1-->4Gal beta 1-->4GlcNAc, Gal beta 1-->4Gal beta 1-->6GlcNAc, and Gal beta 1-->4Gal beta 1-->3GalNAc for use in the total synthesis of complex sugar chains.
Fujimoto
H
H
Meiji Institute of Health Science, Naruda Odawara, Japan.
Nakano
H
H
Isomura
M
M
Kitahata
S
S
Ajisaka
K
K
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000096:biosynthesis*; D006026:Glycoside Hydrolases; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis*; D010407:Penicillium / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism*
D004187:Disaccharides; D009844:Oligosaccharides; D006026:Glycoside Hydrolases; C089138:beta-galactanase; D001616:beta-Galactosidase