title: Two distinct human uterine cervical epithelial cell lines established after transfection with human papillomavirus 16 DNA.
abstract: We have established two distinct human cervical cell lines, NCC16 and NCE16, after transfecting human papillomavirus type 16 (HPV16) DNA into normal human ecto-cervical and endo-cervical epithelial cells, respectively. Both lines expressed HPV16 E6 and E7 as detected by reverse transcriptase-polymerase chain reaction and northern blot hybridization. These cells have been passaged for over 100 population doublings and express strong telomerase activity. Neither cell line was tumorigenic in athymic nu/nu mice. However, both NCC16 and NCE16 developed abnormally stratified architectures following implantation with a silicon membrane sheet in the back of athymic nude mice. The former cells were pathohistologically similar to carcinoma, while the latter produced Alcian-blue positive cells, suggesting the occurrence of metaplastic changes. These distinct cell lines offer a useful model system for the study of cervical carcinogenesis and of its regulatory mechanism after HPV infection in different regions of the uterine cervix.
authors.affiliation: Department of Gynecology, Nippon Medical School, Tokyo.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
authors.forename: K
authors.initials: K
authors.lastname: Tsutsumi
authors.forename: K
authors.initials: K
authors.lastname: Kikuchi
authors.forename: S
authors.initials: S
authors.lastname: Yasumoto
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology; D002460:Cell Line; D002472:Cell Transformation, Viral; D002584:Cervix Uteri / Q000166:cytology* / Q000502:physiology / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology / Q000821:virology; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D027383:Papillomaviridae / Q000235:genetics*; D010641:Phenotype; D019098:Telomerase / Q000378:metabolism; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology
chemical_list: D004279:DNA, Viral; D019098:Telomerase