Identification of a 105 kilodalton Cryptococcus neoformans mannoprotein involved in human cell-mediated immune response.
In the present study, using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot techniques, the patterns of proteins extracted from C. neoformans yeast cells were analysed. A major 105 kilodalton (kDa) antigen that binds to Concanavalin A and cross-reacts with anti-mannan antibodies was identified. The 105 kDa mannoprotein, highly expressed in the acapsular mutant of C. neoformans with respect to the encapsulated strain, is involved in the lymphoproliferative response of T lymphocytes to Cryptococcus-sensitized monocytes.
Pitzurra
L
L
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Vecchiarelli
A
A
Peducci
R
R
Cardinali
A
A
Bistoni
F
F
D000906:Antibodies; D003208:Concanavalin A; D003429:Cross Reactions; D003455:Cryptococcus neoformans / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D008970:Molecular Weight; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D008562:Membrane Glycoproteins; C025363:mannoproteins; D003208:Concanavalin A