Ultrastructure of Fos-labeled neurons relating to nociceptive primary afferent and substance P terminals in rat spinal superficial laminae.
The presence of Fos-labeled neurons at ultrastructural level was confirmed in the spinal superficial laminae following an injection of formalin into rat hindpaw in the present study. The Fos-like immunoreactive products were found exclusively in regions associated with the euchromatin in the nuclei of Fos-labeled neurons. By the methods used-of anterograde transport of horseradish peroxidase conjugated to wheat-germ agglutinin and immunocytochemistry-it was observed that some Fos-labeled neuronal bodies received synaptic contacts from, or were apposed directly to, small diameter primary afferent terminals in the spinal superficial laminae. By means of double-labeled immunocytochemistry, a direct apposition was often observed and a synaptic relationship was occasionally found between Fos-labeled neuronal bodies and substance P-like immunoreactive terminals.
Tao
Y X
YX
Shanghai Brain Research Institute, Chinese Academy of Sciences, People's Republic of China.
Zhao
Z Q
ZQ
D000344:Afferent Pathways / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000378:metabolism / Q000648:ultrastructure*; D009475:Neurons, Afferent / Q000378:metabolism / Q000648:ultrastructure*; D009619:Nociceptors / Q000378:metabolism / Q000648:ultrastructure*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism / Q000648:ultrastructure*; D013373:Substance P / Q000276:immunology / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D013373:Substance P