title: Characterization of membrane surface proteins of Mycoplasma agalactiae during natural infection.
abstract: We have analyzed antigenic variation of seven M. agalactiae wild strains using different sera from naturally infected sheep. Only 30 day sera recognized all surface proteins and inhibited the growth of mycoplasmas. Furthermore, we have observed that two strongly immunogenic proteins: 55 and 35 kDa were digested using 500 micrograms/ml of trypsin. These two bands are immunoprecipitated together with four other proteins but only the 35 kDa protein is recognized by eluted antibodies.
authors.affiliation: Istituto Zooprofilattico Sperbnentale della Sardegna G Pegreffi, Sassari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Tola
authors.forename: D
authors.initials: D
authors.lastname: Manunta
authors.forename: M
authors.initials: M
authors.lastname: Cocco
authors.forename: F
authors.initials: F
authors.lastname: Turrini
authors.forename: A M
authors.initials: AM
authors.lastname: Rocchigiani
authors.forename: G
authors.initials: G
authors.lastname: Idini
authors.forename: A
authors.initials: A
authors.lastname: Angioi
authors.forename: G
authors.initials: G
authors.lastname: Leori
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D008970:Molecular Weight; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology*; D011233:Precipitin Tests; D012756:Sheep
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface