[A comparative phylogenetic analysis of the coding portion of the genes for structural protein E1 in the Shi-Min' strain and in other strains of the classic hog cholera virus].
The primary nucleotide sequence of the fragment of E1 (gp51-55) gene (772 bp in length) of virulent strain Shi-Min' of classical swine fever virus (CSFV) has been determined. Multiple alignments of the E1 gene fragments of various strains and isolates of CSFV, which are homologues to the cloned fragment, has been carried out; the phylogenetic tree has been plotted and the consensus sequence has been determined. The strain Shi-Min', which was used as a control CSFV strain in former Soviet republics, and the referent strain Alfort were shown to be located on the opposite sides of the dendrogram, while Shi-Min' was more close to the western European group of CSFVs. We suggest that Shi-Min' strain as a positive viral antigen for vaccine testing represents a selective factor for the formation of virus population in Ukraine. The data obtained are the first results in molecular epizootic study of hog cholera in Ukraine.
Kyrylenko
S D
SD
Deriabin
O M
OM
Troianovs'kyĭ
B M
BM
Deriabina
O H
OH
Kyrylenko
O L
OL
Sozinov
O O
OO
D000956:Antigens, Viral / Q000235:genetics; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D016384:Consensus Sequence; D017931:DNA Primers; D005814:Genes, Viral / Q000235:genetics*; D005815:Genetic Code / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D014455:Ukraine; D015678:Viral Structural Proteins / Q000235:genetics*; D014774:Virulence / Q000235:genetics
D000956:Antigens, Viral; D017931:DNA Primers; D015678:Viral Structural Proteins; C107842:protein E1, Classical swine fever virus