title: Glycine68 to histidine73 has an important role in the function of human tumor necrosis factor alpha.
abstract: Mutant human tumor necrosis factor alpha(hTNF alpha) genes have been constructed by in vitro mutagenesis and expressed in Escherichia coli. A deletion involving Gly68 to His73 in hTNF alpha remarkably decreased the solubility and biological activity of hTNF alpha. From the above and results of a molecular dynamics simulation it is proposed that the region of Gly68 to His73 in hTNF alpha has an important role in the maintenance of the 3-D structure and modulation of the biological activity of hTNF alpha.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, People's Republic of China.
authors.forename: B
authors.initials: B
authors.lastname: Cen
authors.forename: W
authors.initials: W
authors.lastname: Jin
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.forename: X
authors.initials: X
authors.lastname: Dong
authors.forename: X
authors.initials: X
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016923:Cell Death; D002460:Cell Line; D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D017384:Sequence Deletion; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*
chemical_list: D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha