title: [Stabilization of lactate dehydrogenase by polyethylene glycol].
abstract: Polyethylene glycol (PEG) stabilizes lactate dehydrogenase (LDH) from pig muscle. Time of half-inactivation by the heating at 45 degrees C is linearly increased by PEG from 21.6 min up to 114 min without and at 15% PEG presence, respectively. PEG practically does not affect Michaelis constant and Hill coefficient fro pyruvate and decreases the maximal activity. Possible mechanisms of LDH stabilization by PEG is discussed.
authors.forename: V I
authors.initials: VI
authors.lastname: Lushchak
mesh_terms: D000818:Animals; D004795:Enzyme Stability; D006358:Hot Temperature; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000187:drug effects*; D016014:Linear Models; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013552:Swine
chemical_list: D011092:Polyethylene Glycols; D007770:L-Lactate Dehydrogenase