title: Molecular interactions between dynamin and G-protein betagamma-subunits in neuroendocrine cells.
abstract: Dynamin and G-proteins both are guanosine triphosphate (GTP) binding proteins, with dynamin active in cellular membrane trafficking and G-proteins in intracellular signal transduction. Here we demonstrate that dynamin physically and functionally interacts with G-protein betagamma-subunits in neuroendocrine GH4C1 cells, on stimulation with thyrotropin-releasing hormone and somatostatin. The interaction appears to be of high affinity and inhibitory on dynamin GTPase activity, mediated by the pleckstrin homology domain and regulated both by the G-protein alpha-subunit and by guanosine nucleotides. Thus, dynamin may target particular sites for receptor-mediated endocytosis by sharing betagamma-subunits with the alpha subunit of G-proteins in neuroendocrine cells.
authors.affiliation: Department of Medical Oncology, Newcastle Mater Misericordiae Hospital, NSW, Australia. Jun-Ping.Liu@Baker.edu.au
authors.forename: J P
authors.initials: JP
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Yajima
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Ackland
authors.forename: Y
authors.initials: Y
authors.lastname: Akita
authors.forename: J
authors.initials: J
authors.lastname: Stewart
authors.forename: S
authors.initials: S
authors.lastname: Kawashima
mesh_terms: D000818:Animals; D002460:Cell Line; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D034281:Dynamins