GATA-6 DNA binding protein expressed in human gastric adenocarcinoma MKN45 cells.
A cDNA for the GATA-6 (GATA-GT1) DNA binding protein was cloned from a library of the human gastric adenocarcinoma cell line MKN45. The deduced amino acid sequence (449 residues) indicates that the primary structure of human GATA-6 is highly homologous to that of the rat protein. The potential phosphorylation site for protein kinases (A and C), and histidine and alanine clusters are conserved. Whereas the rat H+/K+-ATPase alpha and beta subunit genes have two and three GATA protein binding sites in their promoter regions, respectively, the human alpha subunit gene has only one binding site [Maeda, M., Kubo, K., Nishi, T. and Futai, M. (1996) J. Exp. Biol. 199, 513-520]. We cloned the 5'-upstream region of the human H+/K+-ATPase beta subunit gene by genome walking and found that it also has a single GATA protein binding site near the TATA box. The GATA sites of the human alpha and beta subunit genes are recognized by the zinc finger domain of human GATA-6. The conservation of the GATA protein binding sites suggests that they are important for the gene regulation of the human and rat H+/K+-ATPase.
Yoshida
T
T
Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University, Suita, Japan.
Sato
R
R
Mahmood
S
S
Kawasaki
S
S
Futai
M
M
Maeda
M
M
D000230:Adenocarcinoma / Q000378:metabolism*; D000409:Alanine; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D050993:GATA6 Transcription Factor; D006639:Histidine; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013274:Stomach Neoplasms / Q000378:metabolism*; D013379:Substrate Specificity; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D050993:GATA6 Transcription Factor; C494759:GATA6 protein, human; C494760:Gata6 protein, mouse; D009838:Oligodeoxyribonucleotides; D014157:Transcription Factors; D006639:Histidine; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D000409:Alanine