title: Isolation and characterization of Exodus-2, a novel C-C chemokine with a unique 37-amino acid carboxyl-terminal extension.
abstract: Chemokines are a group of small, homologous proteins that regulate leukocyte migration, hemopoiesis, and HIV-1 absorption. We report here the cloning and characterization of a novel murine and human C-C chemokine termed Exodus-2 for its similarity to Exodus-1/MIP-3alpha/LARC, and its chemotactic ability. This novel chemokine has a unique 36 or 37 (murine and human, respectively) amino acid carboxyl-terminal extension not seen in any other chemokine family member. Purified recombinant Exodus-2 was found to have two activities classically associated with chemokines: inhibiting hemopoiesis and stimulating chemotaxis. However, Exodus-2 also had unusual characteristics for C-C chemokines. It selectively stimulated the chemotaxis of T-lymphocytes and was preferentially expressed in lymph node tissue. The combination of these characteristics may be a functional correlate for the unique carboxyl-terminal structure of Exodus-2.
authors.affiliation: Department of Medicine, Indiana University Medical Center, Indianapolis 46202, USA. robertvhromas@iucc.iupui.edu
authors.forename: R
authors.initials: R
authors.lastname: Hromas
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Klemsz
authors.forename: M
authors.initials: M
authors.lastname: Krathwohl
authors.forename: K
authors.initials: K
authors.lastname: Fife
authors.forename: S
authors.initials: S
authors.lastname: Cooper
authors.forename: C
authors.initials: C
authors.lastname: Schnizlein-Bick
authors.forename: H E
authors.initials: HE
authors.lastname: Broxmeyer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D054421:Chemokine CCL21; D018925:Chemokines / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D019742:Chemokines, CC; D002633:Chemotaxis / Q000187:drug effects; D003001:Cloning, Molecular; D006801:Humans; D007962:Leukocytes / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis
chemical_list: C515478:CCL21 protein, human; C515479:Ccl21c protein, mouse; D054421:Chemokine CCL21; D018925:Chemokines; D019742:Chemokines, CC