title: Inhibitory monoclonal antibodies to human cytochrome P450 2D6.
abstract: Two monoclonal antibodies (MAbs) have been isolated that bind to human P450 2D6 and inhibit 2D6 catalyzed bufuralol 1-hydroxylation by 90%. One but not both of the MAbs immunoblotted 2D6. The MAbs were highly specific to 2D6 and did not cross-react with other P450s. Inhibitory monoclonal antibodies will be useful for determining the contribution of 2D6 to the metabolism of a wide variety of 2D6 and other P450 substrates in human tissues containing multiple P450s.
authors.affiliation: Laboratory of Molecular Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, U.S.A.
authors.forename: K W
authors.initials: KW
authors.lastname: Krausz
authors.forename: T J
authors.initials: TJ
authors.lastname: Yang
authors.forename: F J
authors.initials: FJ
authors.lastname: Gonzalez
authors.forename: M
authors.initials: M
authors.lastname: Shou
authors.forename: H V
authors.initials: HV
authors.lastname: Gelboin
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D019389:Cytochrome P-450 CYP2D6 / Q000096:biosynthesis / Q000276:immunology; D065690:Cytochrome P-450 CYP2D6 Inhibitors; D004983:Ethanolamines / Q000378:metabolism; D006801:Humans; D006900:Hydroxylation; D010616:Phenanthrenes / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D065690:Cytochrome P-450 CYP2D6 Inhibitors; D004983:Ethanolamines; D010616:Phenanthrenes; C031181:phenanthrene; C010831:bufuralol; D019389:Cytochrome P-450 CYP2D6