Mutant frequency at the Hprt locus and in minisatellite sequences in Chinese hamster V79 cells irradiated with low-energy protons (31 keV/microm) and ultraviolet light (254 nm).
Ionizing radiations induce mutations which can be detected both in coding sequences (Hprt locus) by measuring the frequency of 6-thioguanine-resistant cells and in minisatellite sequences by DNA fingerprint analysis. We analyzed the effects of irradiation with low-energy protons (31 keV/pm) and, for comparison, with ultraviolet light (254 nm), for which DNA damage and repair mechanisms are better understood, on cultures of Chinese hamster V79 cells with the two methods mentioned above. The results indicate that the frequency of 6-thioguanine-resistant cells was increased significantly, although very differently, by both treatments. The analyses carried out by DNA fingerprinting with a multilocus DNA probe show that the level of induction in minisatellite sequences was higher compared to those measured at the Hprt locus after proton irradiation, but lower after treatment with ultraviolet light.
Ogheri
S
S
Dipartimento di Biologià, Universita di Padova, Italy.
Bruna
V
V
Cera
F
F
Favaretto
S
S
Cherubini
R
R
Celotti
L
L
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D016172:DNA Fingerprinting; D004307:Dose-Response Relationship, Radiation; D004351:Drug Resistance; D005796:Genes / Q000528:radiation effects*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D018598:Minisatellite Repeats / Q000528:radiation effects*; D016296:Mutagenesis; D011522:Protons; D013866:Thioguanine / Q000633:toxicity; D014466:Ultraviolet Rays
D011522:Protons; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine