title: Effects of long-term corticosterone implants on growth and immune function in juvenile alligators, Alligator mississippiensis.
abstract: Sixty juvenile alligators were implanted subcutaneously with slow release pellets of corticosterone or placebo. Alligators were divided into five different groups such that each group received a different dose. A blood sample was taken prior to and 4 days after the implants were in place to measure hormone levels. Additional blood samples were collected at 1 month and 3 months. At 4 days corticosterone levels ranged from 3,400 ng/ml in the group treated with the high dose to 40 ng/ml in the group implanted with the low dose. The extremely high dose caused 40% mortality within 4 weeks. It was evident that the pellets did not release the hormone for the expected 90 days. Circulating levels of corticosterone were back to baseline levels by 3 months. Hormone levels achieved at 4 days were a reliable predictor of subsequent growth. Rate of growth was negatively correlated with plasma corticosterone at 4 days (r2 = 0.711) and at 1 month (r2 = 0.544) posttreatment. Differential white blood cell counts performed after 1 month of treatment showed a clear effect of the implant. Alligators treated with corticosterone had decreased percentages of lymphocytes, eosinophils, and basophils and had a higher heterophil/lymphocyte (H/L) ratio than the placebo group. Furthermore, histological examination of the spleen revealed a significant depletion of lymphoid cells in alligators treated with the highest dose of hormone. The results from this study demonstrate that exogenous corticosterone can mimic the effects of prolonged stress in juvenile alligators.
authors.affiliation: Center for Reproduction of Endangered Species, Zoological Society of San Diego, California 92112, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Morici
authors.forename: R M
authors.initials: RM
authors.lastname: Elsey
authors.forename: V A
authors.initials: VA
authors.lastname: Lance
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000473:pathology; D000450:Aldosterone / Q000097:blood; D000489:Alligators and Crocodiles / Q000097:blood / Q000502:physiology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects*; D003345:Corticosterone / Q000097:blood / Q000494:pharmacology*; D004343:Drug Implants; D004804:Eosinophils / Q000502:physiology; D007107:Immune System / Q000187:drug effects*; D007962:Leukocytes / Q000502:physiology; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D015996:Survival Rate
chemical_list: D000893:Anti-Inflammatory Agents; D001786:Blood Glucose; D004343:Drug Implants; D000450:Aldosterone; D003345:Corticosterone