One-step transformation of the dimorphic yeast Yarrowia lipolytica.
An efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36-48 h after plating. The efficiency was better than 10(5) transformants/micrograms replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated.
Chen
D C
DC
Laboratoire de Genétique Moléculaire et Cellulaire, Institut National de la Recherche Agromique-CNRS, Institut National Agronomique Paris-Grignon, Thiverval-Grignon, Paris, France.
Beckerich
J M
JM
Gaillardin
C
C
D004718:Saccharomycetales / Q000235:genetics*; D014170:Transformation, Genetic