title: oxyR-dependent induction of Escherichia coli grx gene expression by peroxide stress.
abstract: The Escherichia coli OxyR protein is a transcriptional activator for a number of genes induced in response to low concentrations of hydrogen peroxide. To identify additional OxyR-regulated genes, I cloned a DNA fragment that shows promoter activity regulated by OxyR by direct selection of OxyR-binding DNA fragments. Analyses of the cloned fragment indicate that the grx gene, encoding glutaredoxin 1, is inducible by hydrogen peroxide in an oxyR-dependent fashion.
authors.affiliation: Radioisotope Center, University of Tokyo, Japan. tao@ric.utokyo.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Tao
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D054477:Glutaredoxins; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases; D011506:Proteins / Q000235:genetics*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics*; D015183:Restriction Mapping; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D054477:Glutaredoxins; D011506:Proteins; D012329:RNA, Bacterial; D012097:Repressor Proteins; D014157:Transcription Factors; C499299:oxyR protein, E coli; D006861:Hydrogen Peroxide; D010088:Oxidoreductases