The effects of cyclic fatty acid monomers on cultured porcine endothelial cells.
The popularity of polyunsaturated oils used in food applications and preparation continues to appreciate as a result of positive health claims. With polyunsaturated oils inherently more susceptible to oxidative and thermal degradation, the formation of new fatty acid species increases considerably. The presence of one species known as cyclic fatty acid monomers (CFAM) has been detected as a component of many oils subjected to various thermal processes including deep-fat frying. The effect of CFAM on metabolic processes has not been fully characterized. In this study, confluent porcine aortic endothelial cells incorporated CFAM into their polar and nonpolar lipid fractions following a 48-h exposure to 31 and 62 ppm CFAM in the culture medium. Subsequently, the influence of CFAM incorporation on various membrane-dependent physical properties and biochemical processes was investigated. CFAM decreased the lipid packing order of the membrane bilayer core but did not alter the lipid packing order of lipid chain segments at or near the lipid-water interface of the membrane. CFAM led to significant reductions in Ca2+ ATPase activity and monolayer integrity while eliciting a significant increase of prostacyclin synthesis and secretion.
Flickinger
B D
BD
Division of Nutritional Sciences, University of Illinois at Urbana-Champaign 61801, USA.
McCusker
R H
RH
Perkins
E G
EG
D000818:Animals; D001011:Aorta; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D011464:Epoprostenol / Q000096:biosynthesis; D005227:Fatty Acids / Q000737:chemistry / Q000633:toxicity*; D005454:Fluorescence Polarization; D006358:Hot Temperature; D006865:Hydrogenation; D008043:Linseed Oil / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry; D015394:Molecular Structure; D013552:Swine
D005227:Fatty Acids; D008563:Membrane Lipids; D008043:Linseed Oil; D011464:Epoprostenol; D000252:Calcium-Transporting ATPases