title: Isolation and characterization of Enterobacter cloacae mutants which are defective in chemotaxis toward inorganic phosphate.
abstract: Enterobacter cloacae IFO3320 is attracted to Pi when cells are starved for Pi. Two Tn1737KH-induced mutants, which were constitutive for alkaline phosphatase, failed to exhibit Pi taxis even under conditions of Pi limitation. Both of the mutant strains exhibited normal chemotactic responses to peptone, suggesting that they are specifically defective in Pi taxis. Cloning and sequence analysis showed that the TN1737KH insertions were located in either the pstA or pstB genes which encode the channel-forming proteins of the Pi-specific transport (Pst) system in E. cloacae. These results suggest that the E. cloacae Pst system is required for Pi chemoreception.
authors.affiliation: Department of Fermentation Technology, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kusaka
authors.forename: K
authors.initials: K
authors.lastname: Shibata
authors.forename: A
authors.initials: A
authors.lastname: Kuroda
authors.forename: J
authors.initials: J
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Ohtake
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001692:Biological Transport; D002633:Chemotaxis; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D016972:Enterobacter cloacae / Q000235:genetics / Q000502:physiology*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D009876:Operon; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D010710:Phosphates; C078408:phosphate-specific transport protein A, bacteria; D000469:Alkaline Phosphatase