title: Cyclin D1 protein in multiple myeloma and plasmacytoma: an immunohistochemical study using fixed, paraffin-embedded tissue sections.
abstract: In this study, we analyzed 69 plasma cell neoplasms, including 54 multiple myelomas (MMs), 3 cases of plasma cell leukemia, and 12 plasmacytomas, for expression of cyclin D1 protein using an immuno-histochemical method applied to routinely fixed, paraffin-embedded tissue sections. Cyclin D1 was expressed in 18 (26%) of 69 plasma cell neoplasms, including 16 (30%) of 54 MMs and 2 (17%) of 12 plasmacytomas. Three cases of plasma cell leukemia were negative. Cyclin D1 expression correlated with the cytologic differentiation and histologic stage of MM. Twelve (75%) of 16 MMs had intermediate or immature cytologic features and were histologic Stage III. With use of a polymerase chain reaction assay, we also analyzed six cases (three cyclin D1 positive, three cyclin D1 negative) for the t(11;14); one MM carried the t(11;14) and expressed cyclin D1 protein. We conclude that cyclin D1 expression occurs in approximately one-quarter of plasma cell neoplasms and correlates with the degree of cytologic differentiation and histologic stage. The relatively high frequency of cyclin D1 expression, compared with the less than 5% incidence of the t(11;14) detected by conventional cytogenetics reported in the literature, suggests that upregulation of cyclin D1 protein might be the result of mechanisms other than the t(11;14).
authors.affiliation: Division of Pathology, City of Hope National Medical Center, Duarte, CA 91010, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Vasef
authors.forename: L J
authors.initials: LJ
authors.lastname: Medeiros
authors.forename: L S
authors.initials: LS
authors.lastname: Yospur
authors.forename: N C
authors.initials: NC
authors.lastname: Sun
authors.forename: A
authors.initials: A
authors.lastname: McCourty
authors.forename: R K
authors.initials: RK
authors.lastname: Brynes
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019938:Cyclin D1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007150:Immunohistochemistry; D007952:Leukemia, Plasma Cell / Q000276:immunology / Q000378:metabolism / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology / Q000378:metabolism* / Q000401:mortality; D016612:Paraffin Embedding; D010954:Plasmacytoma / Q000276:immunology / Q000378:metabolism* / Q000401:mortality; D016133:Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D019938:Cyclin D1