title: Characterization of microsomal GST-II by western blot and identification of a novel LTC4 isomer.
abstract: Protein expression of microsomal GST-II and LTC4 synthase was analyzed by Western blot. Correlation between a 17 kDa band and LTC4 formation was observed for both enzymes. The expression of microsomal GST-II was several fold more efficient than the expression of LTC4 synthase. In addition to catalyzing the biosynthesis of LTC4, microsomal GST-II also produces another product, which has been subjected to mass spectrometric analysis. This analysis demonstrates that the novel product is an isomer of LTC4.
authors.affiliation: Merck Frosst Centre For Therapeutic Research, Kirkland, Quebec, Canada. Per-Johan.Jakobsson@mbb.ki.se
authors.forename: P J
authors.initials: PJ
authors.lastname: Jakobsson
authors.forename: K A
authors.initials: KA
authors.lastname: Scoggan
authors.forename: J
authors.initials: J
authors.lastname: Yergey
authors.forename: J A
authors.initials: JA
authors.lastname: Mancini
authors.forename: A W
authors.initials: AW
authors.lastname: Ford-Hutchinson
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D007536:Isomerism; D017997:Leukotriene C4 / Q000737:chemistry*; D013058:Mass Spectrometry; D008861:Microsomes / Q000201:enzymology*; D018411:Spodoptera
chemical_list: D017997:Leukotriene C4; D005982:Glutathione Transferase; C041116:leukotriene-C4 synthase