title: Analytical method for the determination of urinary 3-phenoxybenzoic acid in subjects occupationally exposed to pyrethroid insecticides.
abstract: The determination of urinary 3-phenoxybenzoic acid enables exposure to pyrethroid insecticides to be evaluated. A method for the quantitative determination of this metabolite in urine is described. The compound and the internal standard (2-phenoxybenzoic acid) are derivatized with pentafluorobenzylbromide and transformed into pentafluorobenzyl esters, which are determined by gas chromatography with an intermediate polarity capillary column and an electron-capture detector. Before GC analysis, the urinary extracts are purified on LC-Si SPE columns. The proposed method has a detection limit of 0.5 microg/l and a mean recovery of 91.3%. The coefficient of variation of the analytical procedure, evaluated at a concentration of 24.96 microg/l, was 9.58%. Storage of the urine samples for 3 months at -18 degrees C did not lead to significant changes in the concentration of analyte. The method was tested analysing the urine of a farm worker with symptoms of pyrethroid poisoning, occupationally exposed to esfenvalerate.
authors.affiliation: Institute of Occupational Medicine, University of Siena, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Aprea
authors.forename: A
authors.initials: A
authors.lastname: Stridori
authors.forename: G
authors.initials: G
authors.lastname: Sciarra
mesh_terms: D000395:Air Pollutants, Occupational / Q000378:metabolism / Q000506:poisoning; D001565:Benzoates / Q000652:urine*; D002849:Chromatography, Gas; D006801:Humans; D007306:Insecticides / Q000378:metabolism* / Q000506:poisoning; D008297:Male; D009570:Nitriles; D011722:Pyrethrins / Q000378:metabolism* / Q000506:poisoning; D012680:Sensitivity and Specificity
chemical_list: D000395:Air Pollutants, Occupational; D001565:Benzoates; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017618:3-phenoxybenzoic acid; C017690:fenvalerate