Secretion of Porphyromonas gingivalis fimbrillin polypeptides by recombinant Streptococcus gordonii.
The fimbriae of Porphyromonas gingivalis plays an important role in the pathogenesis of periodontal disease. A structural subunit of the P. gingivalis fimbriae, fimbrillin, has been shown to promote adherence of the bacteria to host surfaces and also induce an immune response. Biologically active domains of fimbrillin responsible for adherence or eliciting immune responses have been determined. In a previous study, we engineered the human oral commensal organism Streptococcus gordonii to express such biologically active domains on the surface of the bacteria as a vaccine delivery system. In this study we report an alternative approach of secreting fimbrillin polypeptide domains into the medium by modification of the surface-expression system described earlier. Such recombinant S. gordonii, in addition to being a source for antigen presentation to trigger a protective immune response, may have the added advantage of directly blocking the fimbriae-mediated adherence of P. gingivalis to the oral cavity following implantation. This approach can also be utilized for secreting other biologically important therapeutic molecules on mucosal surfaces for modulating local microenvironments.
Sharma
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A
School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York 14214, USA. sharmaa@acsu.buffalo.edu
Sojar
H T
HT
Hruby
D E
DE
Kuramitsu
H K
HK
Genco
R J
RJ
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant; D036781:Fimbriae Proteins; D006801:Humans; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013291:Streptococcus / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D011994:Recombinant Proteins; C063055:fimbrillin; D036781:Fimbriae Proteins