title: Bluetongue virus VP6 protein binds ATP and exhibits an RNA-dependent ATPase function and a helicase activity that catalyze the unwinding of double-stranded RNA substrates.
abstract: RNA-dependent ATPase and helicase activities have been identified associated with the purified VP6 protein of bluetongue virus, a member of the Orbivirus genus of double-stranded RNA (dsRNA; Reoviridae family) viruses. In addition, the protein has an ATP binding activity. RNA unwinding of duplexes occurred with both 3' and 5' overhang templates, as well as with blunt-ended dsRNA, an activity not previously identified in other viral helicases. Although little sequence similarity to other helicases was detected, certain similarities to motifs commonly attributed to such proteins were identified.
authors.affiliation: Department of Biochemistry, Oxford University, United Kingdom.
authors.forename: N
authors.initials: N
authors.lastname: Stäuber
authors.forename: J
authors.initials: J
authors.lastname: Martinez-Costas
authors.forename: G
authors.initials: G
authors.lastname: Sutton
authors.forename: K
authors.initials: K
authors.lastname: Monastyrskaya
authors.forename: P
authors.initials: P
authors.lastname: Roy
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D001820:Bluetongue virus / Q000201:enzymology*; D002213:Capsid / Q000096:biosynthesis / Q000378:metabolism*; D036022:Capsid Proteins; D002460:Cell Line; D007700:Kinetics; D009838:Oligodeoxyribonucleotides; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D018411:Spodoptera; D013379:Substrate Specificity; D013698:Templates, Genetic; D014162:Transfection
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D009838:Oligodeoxyribonucleotides; D012330:RNA, Double-Stranded; D011994:Recombinant Proteins; C061332:VP6 protein, Rotavirus; D000255:Adenosine Triphosphate; D012316:RNA Nucleotidyltransferases; D000251:Adenosine Triphosphatases; C027900:RNA-dependent ATPase; D020365:RNA Helicases