Copy number of the 16S rRNA gene in Coxiella burnetii.
Coxiella burnetii is an obligate intracellular bacterium with a doubling time of 5-7 hours. Chromosomal DNA from C. burnetii was digested with various restriction enzymes previously determined to not cut within the genomic 16S rRNA gene, or with a combination of these noncutting enzymes in conjunction with AflIII, a restriction enzyme that cuts twice within the 16S rRNA gene. Restriction fragments were resolved electrophoretically and probed with a radiolabeled DNA fragment containing the 3' AflIII portion of the C. burnetii 16S rRNA gene. Only a single DNA fragment in these digests hybridized to the probe, indicating that there is a single genomic copy of the 16S rRNA gene in C. burnetii and thus only a single copy of the rRNA operon.
Afseth
G
G
Department of Microbiology, Washington State University, Pullman 99164-4233, USA.
Mallavia
L P
LP
D016997:Coxiella burnetii / Q000235:genetics* / Q000254:growth & development; D004262:DNA Restriction Enzymes; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012407:rRNA Operon
D004262:DNA Restriction Enzymes