title: Regulation of insulin-like growth factor-binding protein-1 and progesterone secretion from human granulosa-luteal cells: effects of octreotide and insulin.
abstract: OBJECTIVE
  To examine the effects of the synthetic somatostatin-analogue octreotide and human recombinant insulin on the release of insulin-like growth factor binding protein-1 (IGFBP-1) and P from human granulosa-luteal cells.
  
  
  METHODS
  Primary culture of human granulosa-luteal cells.
  
  
  METHODS
  Academic research laboratory.
  
  
  METHODS
  Women undergoing oocyte retrieval for IVF-ET because of tubal infertility.
  
  
  METHODS
  Octreotide or insulin were added to the cultures; sampling of culture medium was performed after 48 hours.
  
  
  METHODS
  Insulin-like growth factor binding protein-1 and P.
  
  
  RESULTS
  Octreotide significantly inhibited IGFBP-1 (58.8% compared with controls) and P release (66.1% compared with controls). Insulin abolished IGFBP-1 release while stimulating P release (200.7% compared with controls). There was a significant and positive correlation between IGFBP-1 and P levels.
  
  
  CONCLUSIONS
  Octreotide and insulin have a significant effect on human granulosa-luteal cell function in terms of IGFBP-1 and P release. Our results suggest a local ovarian mechanism for the recently observed effects of octreotide in the treatment of women with polycystic ovary syndrome.
authors.affiliation: Department of Obstetrics and Gynecology, Ulleval Hospitals, University of Oslo, Norway.
authors.forename: N
authors.initials: N
authors.lastname: Holst
authors.forename: K H
authors.initials: KH
authors.lastname: Kierulf
authors.forename: M
authors.initials: M
authors.lastname: Seppälä
authors.forename: R
authors.initials: R
authors.lastname: Koistinen
authors.forename: M B
authors.initials: MB
authors.lastname: Jacobsen
mesh_terms: D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000378:metabolism*; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism; D015282:Octreotide / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism*
chemical_list: D007328:Insulin; D018970:Insulin-Like Growth Factor Binding Protein 1; D011374:Progesterone; D015282:Octreotide