title: [Stimulation of growth and spore formation of Bacillus subtilis by optimization of carbohydrate nutrition during submerged cultivation].
abstract: A wide range of carbohydrate sources for the growth of industrial Bacillus subtilis strains were studied. The growth was mainly accelerated 2.7 and 2.5-fold by adding green syrup and glucose in a combination with xylose, respectively, in comparison to the traditional glucose. The revealed nutritional demands of these bacteria allowed us to select an optimal composition of the medium using a method of experimental design. Conditions essential for the maximum production of cells and spores were determined, and certain similarities and strain-specific features of this process were found for the strains studied. Mathematical models describing growth and spore formation during submerged cultivation of Bacillus subtilis were also proposed in this work. The antagonistic activity possessed by the strains studied was shown to be unchanged through batch cultivation. We demonstrated the possibility of direct regulation of Bacillus subtilis growth and spore formation by maintaining the concentration of carbon and nitrogen sources and other components of growth media at a certain level.
authors.forename: A I
authors.initials: AI
authors.lastname: Osadchaia
authors.forename: V A
authors.initials: VA
authors.lastname: Kudriavtsev
authors.forename: L A
authors.initials: LA
authors.lastname: Safronova
authors.forename: I A
authors.initials: IA
authors.lastname: Kozachko
authors.forename: V V
authors.initials: VV
authors.lastname: Smirnov
mesh_terms: D001412:Bacillus subtilis / Q000254:growth & development* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D003470:Culture Media; D008954:Models, Biological; D013170:Spores
chemical_list: D003470:Culture Media