title: Transforming growth factor beta 1, interleukin-8 and interleukin-1, in non-small-cell lung tumors.
abstract: A role in tumor progression has been proposed for transforming growth factor-beta 1 (TGF beta 1) and interleukin (IL)-8 as well as for IL-1, which itself induces the production of TGF beta 1 and IL-8 in many cell types. TGF beta 1 and IL-8 production and their regulation by IL-1 in five non-small-cell (NSC) lung tumor cell lines were evaluated. Moreover, their levels were evaluated in 29 NSC lung tumors. All cell lines constitutively produced TGF beta 1, and three produced IL-8. After IL-1 beta treatment, TGF beta 1 production was upregulated in two cell lines, whereas IL-8 production was markedly upregulated in two, induced in one, and unmodified in two. In tumors, the levels of TGF beta 1, IL-8, and IL-1 beta were higher than in normal counterparts (p < 0.001), and a positive correlation between IL-8 and IL-1 beta levels (p < 0.001) was found. TGF beta 1, IL-8, and IL-1 beta mRNA expression was examined in 12 tumors. TGF beta 1 mRNA was detected in all cases, IL-8 mRNA in 7, and IL-1 beta MRNA was undetectable. TGF beta 1, IL-8, and IL-1 beta immunoreactivity was then studied by immunohistochemistry. TGF beta 1 and IL-8 immunoreactivity was observed in neoplastic cells; IL-1 beta immunoreactivity was observed in mononuclear cells. In conclusion, in tumors IL-1 beta levels positively correlated with those of IL-8, and IL-1 beta as well as TGF beta 1 and IL-8 levels were significantly higher than in normal tissues.
authors.affiliation: Department of Pathology, G. D'Annunzio University, Chieti, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Colasante
authors.forename: N
authors.initials: N
authors.lastname: Mascetra
authors.forename: M
authors.initials: M
authors.lastname: Brunetti
authors.forename: G
authors.initials: G
authors.lastname: Lattanzio
authors.forename: M
authors.initials: M
authors.lastname: Diodoro
authors.forename: S
authors.initials: S
authors.lastname: Caltagirone
authors.forename: P
authors.initials: P
authors.lastname: Musiani
authors.forename: F B
authors.initials: FB
authors.lastname: Aiello
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000032:analysis* / Q000276:immunology; D016209:Interleukin-8 / Q000032:analysis*; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D016212:Transforming Growth Factor beta / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016212:Transforming Growth Factor beta