title: TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells.
abstract: A novel member of the tumor necrosis factor (TNF) cytokine family, designated TRANCE, was cloned during a search for apoptosis-regulatory genes using a somatic cell genetic approach in T cell hybridomas. The TRANCE gene encodes a type II membrane protein of 316 amino acids with a predicted molecular mass of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL, and TNF. TRANCE is an immediate early gene up-regulated by TCR stimulation and is controlled by calcineurin-regulated transcription factors. TRANCE is most highly expressed in thymus and lymph nodes but not in nonlymphoid tissues and is abundantly expressed in T cells but not in B cells. Cross-hybridization of the mouse cDNA to a human thymus library yielded the human homolog, which encodes a protein 83% identical to the mouse ectodomain. Human TRANCE was mapped to chromosome 13q14 while mouse TRANCE was located to the portion of mouse chromosome 14 syntenic with human chromosome 13q14. A recombinant soluble form of TRANCE composed of the entire ectodomain induced c-Jun N-terminal kinase (JNK) activation in T cells but not in splenic B cells or in bone marrow-derived dendritic cells. These results suggest a role for this TNF-related ligand in the regulation of the T cell-dependent immune response.
authors.affiliation: The Rockefeller University, New York, New York 10021, USA.
authors.forename: B R
authors.initials: BR
authors.lastname: Wong
authors.forename: J
authors.initials: J
authors.lastname: Rho
authors.forename: J
authors.initials: J
authors.lastname: Arron
authors.forename: E
authors.initials: E
authors.lastname: Robinson
authors.forename: J
authors.initials: J
authors.lastname: Orlinick
authors.forename: M
authors.initials: M
authors.lastname: Chao
authors.forename: S
authors.initials: S
authors.lastname: Kalachikov
authors.forename: E
authors.initials: E
authors.lastname: Cayani
authors.forename: F S
authors.initials: FS
authors.lastname: Bartlett
authors.forename: W N
authors.initials: WN
authors.lastname: Frankel
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002352:Carrier Proteins; D002460:Cell Line; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D004789:Enzyme Activation; D017781:Genes, Immediate-Early; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases; D008024:Ligands; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis / Q000187:drug effects; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes; D016559:Tacrolimus / Q000494:pharmacology; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D008024:Ligands; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; C505686:TNFRSF11A protein, human; C505688:TNFSF11 protein, human; C505687:Tnfrsf11a protein, mouse; C505689:Tnfsf11 protein, mouse; D003513:Cycloheximide; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D016559:Tacrolimus