Chinese hamster protein homologous to human putative protein kinase KIAA0204 is associated with nuclei, microtubules and centrosomes in CHO-K1 cells.
Monoclonal antibody raised against a preparation of loach fish sperm centrosomes was used for screening of cDNA expressing library of Chinese hamster CHO-K1 cells. Two positive clones appeared to encode 628 amino acid protein fragment that was 72% identical to human KIAA0204 protein, i.e. putative protein kinase. Polyclonal antibodies raised against products of cDNA expression in E. coli recognized 210-kDa polypeptide in CHO-K1 cells and immunostained nuclear speckles, centrosomes and microtubules in these cells. The 210-kDa polypeptide (named MAK-L) co-sedimented with exogenous microtubules. Thus, one more protein kinase seems to be associated with the microtubule network in vertebrate cells.
Zinovkina
L A
LA
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
Poltaraus
A B
AB
Solovyanova
O B
OB
Nadezhdina
E S
ES
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D016466:CHO Cells; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology*; D018385:Centrosome / Q000201:enzymology*; D003001:Cloning, Molecular; D006224:Cricetinae; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017346:Protein Serine-Threonine Kinases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
D017346:Protein Serine-Threonine Kinases