Cloning and sequencing of the hup gene encoding the histone-like protein HSl of Streptomyces lividans.
The hup gene encoding the histone-like HU-type protein HSl of Streptomyces lividans TK24 was cloned and sequenced. The deduced N-terminal amino acid sequence, molecular mass (9851 Da) and pI (9.95) are in good agreement with characteristics of the HSI protein. The hup transcript of about 500 nucleotides was detected. The 2.3-kb HincII fragment containing the hup gene hybridized with the AseI fragment C in the 9-10 o'clock region of the chromosome of S. lividans ZX7.
Yokoyama
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E
Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Doi
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K
Ogata
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S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D012313:RNA