Characterization of the production of acetaldehyde by astrocytes in culture after ethanol exposure.
The nervous system is one of the main targets of ethanol toxicity. Astrocytes might play an important role in ethanol-induced brain toxicity, because their integrity is essential for the normal growth and functioning of neurons. On the other hand, acetaldehyde has been implicated as a mediator in some of the biochemical, pharmacological, and behavioral effects of ethanol. The present study aimed at demonstrating the ability of astrocytes in culture to produce acetaldehyde from ethanol. Significant metabolization of ethanol with production of acetaldehyde was demonstrated in the primary culture of astrocytes. This production was quite low, compared with that usually observed in hepatocytes, but was in the same range as that measured in whole brain homogenates and corresponded to biologically active levels. Such a demonstration could bring new elements for understanding of ethanol neurotoxicity.
Eysseric
H
H
Laboratoire de Neurobiologie du Développement (EA DRED 589), CERMO, Université J. Fourier, France.
Gonthier
B
B
Soubeyran
A
A
Bessard
G
G
Saxod
R
R
Barret
L
L
D000079:Acetaldehyde / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D000431:Ethanol / Q000493:pharmacokinetics* / Q000633:toxicity; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000431:Ethanol; D000079:Acetaldehyde