title: RU 41740 (Biostim) stimulates the production of granulocyte macrophage colony-stimulating factor and interleukin-8 by human bronchial epithelial cells in vitro.
abstract: 1. In this study, we observed the effects of RU 41740 (Biostim) on the production of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-8 (IL-8) in human bronchial epithelial cells in vitro. 2. Cytokine production was assessed by enzyme-linked immunosorbent assay. 3. We report that epithelial cells spontaneously released both cytokines and that RU 41740 induced a significant increase in production of IL-8 and GM-CSF. 4. This is the first observation of a stimulatory effect of an immunostimulating compound used in humans on cytokine production by epithelial cells.
authors.affiliation: Service de Pneumologie, Centre Hospitalier Lyon-Sud, Pierre Bénite, France.
authors.forename: R
authors.initials: R
authors.lastname: Aloui
authors.forename: F
authors.initials: F
authors.lastname: Gormand
authors.forename: B
authors.initials: B
authors.lastname: Guibert
authors.forename: F
authors.initials: F
authors.lastname: Corallo
authors.forename: M
authors.initials: M
authors.lastname: Perrin Fayolle
authors.forename: Y
authors.initials: Y
authors.lastname: Pacheco
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000368:Aged; D001426:Bacterial Proteins / Q000494:pharmacology*; D001980:Bronchi / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged
chemical_list: D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D016209:Interleukin-8; C037567:glycoprotein, Klebsiella pneumoniae; D016178:Granulocyte-Macrophage Colony-Stimulating Factor