title: The dynamics of nitric oxide release measured directly and in real time following repeated waves of cortical spreading depression in the anaesthetised cat.
abstract: Cortical application of crystalline KCl in male cats anaesthetised with alpha-chloralose induced four transient negative deflections in cortical direct current (d.c.) potential. In vehicle treated animals d.c. shifts were associated with a hyperaemia and a multiphasic nitric oxide (NO) release. In these animals, the first negative shift in d.c. potential produced a significantly larger NO electrode current, when compared to subsequent cortical depolarisations. However, regional cerebrovascular laser Doppler flux (rCBF[LDF]) increases were similar for each event. In L-N(G)-nitro-L-arginine (L-NAME; 10 mg/kg i.v. infused over 30 min) treated animals, d.c. shifts were also associated with NO release following a multiphasic waveform and increase in rCBF(LDF), but were significantly attenuated when compared to controls.
authors.affiliation: Neuroscience Research, SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Read
authors.forename: M I
authors.initials: MI
authors.lastname: Smith
authors.forename: A J
authors.initials: AJ
authors.lastname: Hunter
authors.forename: A A
authors.initials: AA
authors.lastname: Parsons
mesh_terms: D000758:Anesthesia; D000818:Animals; D002415:Cats; D013181:Cortical Spreading Depression / Q000187:drug effects / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester