title: Differences in cytokine responses to Onchocerca volvulus extract and recombinant Ov33 and OvL3-1 proteins in exposed subjects with various parasitologic and clinical states.
abstract: Subjects with generalized onchocerciasis (GEN), with the sowdah form, and with exposure but without onchocerciasis (endemic normal/putatively immune; EN/PI) were studied for cytokine responses to Onchocerca volvulus extract (OvAg) and recombinant Ov33 and OvL3-1 proteins. Higher levels of cytokines were produced in response to OvAgs in sowdah and EN/PI than in GEN subjects. Peripheral blood mononuclear cells did not produce interferon-gamma in response to antigens. OvAg induced interleukin (IL)-5, IL-2, granulocyte-macrophage colony-stimulating factor (GM-CSF), and soluble IL-2 receptor. EN/PI and sowdah persons produced significantly more IL-5 and IL-2 than GEN subjects, and EN/PI subjects had significantly higher GM-CSF levels than GEN persons. The low IL-5 and GM-CSF levels in GEN subjects were increased by addition of exogenous IL-2. Ov33 and OvL3-1 stimulated production of IL-10 and less IL-5 and IL-2. The study groups did not show a strict Th2-like cytokine response.
authors.affiliation: Department of Clinical Chemistry, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Brattig
authors.forename: C
authors.initials: C
authors.lastname: Nietz
authors.forename: S
authors.initials: S
authors.lastname: Hounkpatin
authors.forename: R
authors.initials: R
authors.lastname: Lucius
authors.forename: F
authors.initials: F
authors.lastname: Seeber
authors.forename: U
authors.initials: U
authors.lastname: Pichlmeier
authors.forename: T
authors.initials: T
authors.lastname: Pogonka
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D015801:Helminth Proteins / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D017181:Onchocerca volvulus / Q000276:immunology*; D009855:Onchocerciasis / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000494:pharmacology
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D015801:Helminth Proteins; D008934:Mitogens; C097555:OvL3-1 protein, Onchocerca volvulus; D010835:Phytohemagglutinins; D011993:Recombinant Fusion Proteins