The tomato RBCS3A promoter requires integration into the chromatin for correct organ-specific regulation.
In tomato, the RBCS1, RBCS2 and RBCS3A genes, encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, are expressed in leaves and light-grown seedlings, but only RBCS1 and RBCS2 are expressed in developing tomato fruits. The activities of the three promoters have been compared in transgenic plants and after transient transformation. Fruit-specific repression of the RBCS3A promoter was observed in transgenic plants, but not after ballistic transient transformation, indicating that chromatin integration is necessary for its correct organ-specific regulation. In addition, matrix attachment regions have been identified in the RBCS1, RBCS2 and RBCS3A promoters. This is the second case in plants of absence of correct regulation of a plasmid-borne plant promoter and correlating potential nuclear matrix attachment of the gene.
Meier
I
I
Institute for General Botany, AMP I, University of Hamburg, Germany. meieri@esvax.dnet.dupont.com
Gröning
B
B
Michalowski
S
S
Spiker
S
S
D034961:Antigens, Nuclear; D019470:Biolistics; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D017930:Genes, Reporter; D018551:Solanum lycopersicum / Q000201:enzymology / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D014170:Transformation, Genetic
D034961:Antigens, Nuclear; D002843:Chromatin; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012273:Ribulose-Bisphosphate Carboxylase