Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria.
Three insertion sequences were characterized from the widely-used cyanobacterium Synechocystis PCC6803. They all harbored a putative transposase sequence flanked by two imperfect inverted repeats, seemed to have duplicated their target insertion site and occurred as multiple copies in the host genome. They exhibited no obvious homology with any other cyanobacterial ISs and were termed IS5S (871 bp), IS4S (1299 bp) and ISS1987 (949 bp) because they were, respectively, homologous to IS5- and IS4-bacterial elements, and to several members of the IS630-Tc1-mariner superfamily of IS elements occurring in a wide range of hosts. This suggests that these IS-elements were spread through horizontal transfer between evolutionary distant organisms. Three IS5S-copies were isolated as a rescue insertion into a replicating plasmid (IS5Sa), or subsequently cloned from a Synechocystis DNA-library probed with IS5Sa (IS5Sb and IS5Sc), and appeared to be almost identical. In the vicinity of IS5Sb, we found the ISS1987 element inserted into the IS4S element. This indicates that the ISS1987 element has been, and could still be, mobile since its transposase sequence is not interrupted with stop codons or translational frameshifts, unlike that which is found in most members of the IS630-Tc1-mariner superfamily of transposable elements.
Cassier-Chauvat
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C
Service de Biochimie et Génétique Moléculaire, CEA Saclay, URA 2096 CNRS, Gif sur Yvette, France. chauvat@jonas.saclay.cea.fr
Poncelet
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M
Chauvat
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F
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018388:Codon, Terminator; D000458:Cyanobacteria / Q000235:genetics*; D015342:DNA Probes; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D018965:Frameshifting, Ribosomal; D022761:Gene Transfer, Horizontal; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D019895:Transposases / Q000235:genetics
D018388:Codon, Terminator; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019895:Transposases