title: Merits of secretion of heterologous proteins from industrial microorganisms.
abstract: The heterologous expression of proteins is without doubt one of the most fascinating applications of the recombinant DNA technique. Despite clear successes many attempts to produce a certain protein in a heterologous host cell have met with technical difficulties. Secretion from cells has been used as a solution to overcome the intracellular formation of inactive protein. Microorganisms with a history of use in the fermentation industry exhibit clear advantages over the frequently used Escherichia coli as host cells for secreted products. Interleukin-3, chymosin and phytase are examples of commercial products that are produced efficiently with the aid of industrial microorganisms.
authors.affiliation: Genencor Internationa B.V. Delft, The Netherlands.
authors.forename: W J
authors.initials: WJ
authors.lastname: Quax
mesh_terms: D010832:6-Phytase / Q000378:metabolism; D000595:Amino Acid Sequence; D012085:Chymosin / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods*; D007377:Interleukin-3 / Q000378:metabolism; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D007377:Interleukin-3; D011994:Recombinant Proteins; D010832:6-Phytase; D012085:Chymosin