title: Intrarenal oxygen tension measured by a modified clark electrode at normal and low blood pressure and after injection of x-ray contrast media.
abstract: The oxygen tension (pO2) in the rat kidney was studied using a Clark microelectrode with a guard cathode behind the sensing cathode. The mean (+/- SEM) outer tip diameter of the electrodes used was 5.5 +/- 1.9 microm. The zero-pO2 current amounted to 12.5 +/- 0.9 pA at 37 degrees C; at air saturation it was 252 +/- 22.9 pA. Rats with a systolic blood pressure (BP) above 80 mmHg (where 1 mmHg = 133 Pa) showed an average pO2 in the cortex of 45 +/- 2 mmHg and in the outer medulla of 31 +/-1 mmHg. In rats with a BP below 80 mmHg a paradoxically high outer medullary pO2 of 40 +/- 4 mmHg was found, while the pO2 in the cortex was 27 +/- 4 mmHg. Changes in pO2 were also noted in the renal cortex and outer medulla after intravenous injections of the x-ray contrast medium diatrizoate (370 mg iodine/ml). In rats with normal BP, injection of diatrizoate caused a slight fall in pO2 in the renal cortex, from 42 +/- 4 to 38 +/-4 mmHg. In the medulla pO2 decreased significantly from 34 +/- 6 to 20 +/-4 mmHg. Ringer's solution did not induce any changes.
authors.affiliation: Department of Diagnostic Radiology, University Hospital, S-751 85 Uppsala, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Liss
authors.forename: A
authors.initials: A
authors.lastname: Nygren
authors.forename: N P
authors.initials: NP
authors.lastname: Revsbech
authors.forename: H R
authors.initials: HR
authors.lastname: Ulfendahl
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D003287:Contrast Media / Q000494:pharmacology*; D003973:Diatrizoate / Q000494:pharmacology*; D006400:Hematocrit; D007022:Hypotension / Q000378:metabolism* / Q000503:physiopathology; D007267:Injections; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008839:Microelectrodes; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D051381:Rats; D011917:Rats, Inbred Lew; D012016:Reference Values; D014556:Urine / Q000737:chemistry
chemical_list: D003287:Contrast Media; D003973:Diatrizoate; D010100:Oxygen