The 28S fraction of rRNA in molluscs displays electrophoretic behaviour different from that of mammal cells.
Electrophoresis on agarose/formaldehyde gels of rRNA in molluscs display a pattern of bands which could suggest a RNase action due to incorrect manipulation of the samples. This study shows that the disappearance of the band corresponding to the 28S fraction is due to the denaturing conditions used when electrophoresis is carried out and not to RNases action.
Barcia
R
R
Departamento de Bioquímica y Biología Molecular, Universidad de Santiago de Compostela, Facultad de Veterinaria, Lugo, Spain. Bmramos@correo.lugo.usc.es
Lopez-García
J M
JM
Ramos-Martínez
J I
JI
D000818:Animals; D049872:Bivalvia / Q000737:chemistry / Q000235:genetics; D001923:Brain Chemistry; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D008297:Male; D008322:Mammals; D008974:Mollusca / Q000737:chemistry* / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar
D012339:RNA, Ribosomal, 28S