The dynamics of nitric oxide release measured directly and in real time following repeated waves of cortical spreading depression in the anaesthetised cat.
Cortical application of crystalline KCl in male cats anaesthetised with alpha-chloralose induced four transient negative deflections in cortical direct current (d.c.) potential. In vehicle treated animals d.c. shifts were associated with a hyperaemia and a multiphasic nitric oxide (NO) release. In these animals, the first negative shift in d.c. potential produced a significantly larger NO electrode current, when compared to subsequent cortical depolarisations. However, regional cerebrovascular laser Doppler flux (rCBF[LDF]) increases were similar for each event. In L-N(G)-nitro-L-arginine (L-NAME; 10 mg/kg i.v. infused over 30 min) treated animals, d.c. shifts were also associated with NO release following a multiphasic waveform and increase in rCBF(LDF), but were significantly attenuated when compared to controls.
Read
S J
SJ
Neuroscience Research, SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
Smith
M I
MI
Hunter
A J
AJ
Parsons
A A
AA
D000758:Anesthesia; D000818:Animals; D002415:Cats; D013181:Cortical Spreading Depression / Q000187:drug effects / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D013997:Time Factors
D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester