Comparison of two methods for the determination of fatty acid profiles in plasma and erythrocytes.
We have validated and compared two methods for the determination of fatty acid profiles in biological samples by capillary gas chromatography. Method I consisted of a previous lipid extraction and esterification of fatty acids using boron trifluoride-methanol. Method II was a direct method that combined extraction and esterification of freeze-dried samples in a single step, using acetyl chloride as the reagent. The two methods were applied to the analysis of plasma and erythrocyte samples. Both methods gave similar results in plasma, whereas in erythrocytes, the direct method gave significantly higher contents of total fatty acids. Precision and recovery rates were determined and the results were satisfactory. Detection and quantification limits showed that both methods had excellent sensitivity. It was concluded that the direct method has substantial advantages over the conventional method, such as higher values in erythrocytes, rapidity and less possibility of contamination or fatty acid losses. Therefore, it is preferable for the analysis of biological samples such as plasma and erythrocytes.
Rodríguez-Palmero
M
M
University of Barcelona, Department of Human Physiological Sciences and Nutrition, Spain.
Lopez-Sabater
M C
MC
Castellote-Bargallo
A I
AI
De la Torre-Boronat
M C
MC
Rivero-Urgell
M
M
D002849:Chromatography, Gas / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry*; D005227:Fatty Acids / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005227:Fatty Acids