The validity of the Aspergillus nidulans linkage map.
The Aspergillus nidulans linkage map is reviewed as a background to physical mapping by cosmid cross-hybridization and genome sequencing. DNA-based methods depend on contiguity, so that the resulting maps are only as good as the weakest link, whereas each new marker added to a linkage map can provide independent confirmation of the positions of its neighbors. For all eight chromosomes of A. nidulans a reliable framework has been provided by analysis of mitotic crossing over, in many cases substantiated by the study of translocation disomics. Building on this framework, there is a backbone of loci linked by reliable three-point meiotic mapping and a second set of less precisely mapped loci. The result is a map with a high degree of self-consistency although some areas of uncertainty or conflict are also noted.
Clutterbuck
A J
AJ
Division of Molecular Genetics, Institute of Biomedical and Life Sciences, Glasgow University, Scotland, United Kingdom.
D001233:Aspergillus nidulans / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods; D015825:Chromosomes, Fungal; D003434:Crossing Over, Genetic; D005800:Genes, Fungal; D008040:Genetic Linkage; D008540:Meiosis; D011995:Recombination, Genetic; D015203:Reproducibility of Results; D014178:Translocation, Genetic