Isolation and characterization of a protein with homology to angiotensin converting enzyme from the periacrosomal plasma membrane of equine spermatozoa.
The periacrosomal plasma membrane of spermatozoa is involved in sperm binding to oviductal epithelial cells and to the zona pellucida. A protein of 68-70 kD molecular mass was purified biochemically from the isolated periacrosomal plasma membrane of equine spermatozoa as a possible receptor for adhesion of spermatozoa to oviductal epithelial cells. A polyclonal antibody raised in rabbits against the purified equine sperm membrane protein recognized the 70 kD and an antigenically related to 32 kD protein in preparations of isolated periacrosomal sperm plasma membrane and in detergent extracted ejaculated and epididymal spermatozoa. A larger protein (approximately 110 kD) was detected in equine testis. Two antigenically related proteins (64 and 45 kD) were recognized on the plasma membrane of cynomolgus macaque spermatozoa. In vitro sperm-binding assays were performed in the presence of antigen-binding fragments or IgG purified from the polyclonal antiserum to investigate a possible function to the isolated protein in binding of equine spermatozoa to homologous oviductal epithelial cells or zona pellucida. Incubation with antigen-binding fragments or IgG purified from the antiserum did not inhibit binding of equine spermatozoa either to oviductal epithelial cells or the zona pellucida. On ultrastructural examination, the antibody bound exclusively to the cytoplasmic side of the periacrosomal plasma membrane of equine and macaque spermatozoa. Microsequence analysis of 13 residues of sequence showed strong homology with a number of angiotensin converting enzymes: An 84% identity was identified with testis specific and somatic forms of human and mouse angiotensin-converting enzyme. Immunocytochemistry and immunoblot analysis established that the protein is specific for the periacrosomal membrane of ejaculated, epididymal, and testicular stallion spermatozoa.
Dobrinski
I
I
Department of Clinical Sciences, Cornell University, Ithaca, New York, USA.
Ignotz
G G
GG
Fagnan
M S
MS
Yudin
S I
SI
Ball
B A
BA
D000177:Acrosome / Q000201:enzymology*; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D004822:Epididymis; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D006736:Horses; D006801:Humans; D007163:Immunosorbent Techniques; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D010449:Peptide Mapping; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011817:Rabbits; D013094:Spermatozoa / Q000378:metabolism / Q000648:ultrastructure; D013737:Testis
D011506:Proteins; D007703:Peptidyl-Dipeptidase A