title: A neurohormone regulating both methyl farnesoate synthesis and glucose metabolism in a crustacean.
abstract: Methyl farnesoate (MF) has been identified as a juvenile hormone-like compound in crustacea which has central roles in the regulation of development and reproduction. To study the regulation of MF synthesis, we isolated a neuropeptide which inhibits MF synthesis from the neurohemal organ-sinus gland X-organ complex of the spider crab Libinia emarginata. The primary structure of this neuropeptide has been determined. It has 72 amino acid residues (deduced molecular mass 8490.5 Da) with pyroglutamic acid at the N-terminus and NH3 at the C-terminus. It shares a high percentage of sequence identity with other sinus gland neuropeptids which form the unique family of CHH neuropeptides of crustacea. Activity studies showed that this neurohormone has dual effects: it inhibited MF synthesis in vitro and had hyperglycemic activity when injected into crabs.
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs 06268, USA.
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Laufer
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Hayes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D007447:Invertebrate Hormones / Q000737:chemistry / Q000494:pharmacology / Q000502:physiology*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000494:pharmacology / Q000502:physiology*; D010446:Peptide Fragments / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005231:Fatty Acids, Unsaturated; D007447:Invertebrate Hormones; D009479:Neuropeptides; D010446:Peptide Fragments; C032429:methyl farnesoate; D010450:Endopeptidases; D005947:Glucose