title: Detection of calves persistently infected with bovine pestivirus in a sample of dairy calves in south-eastern Queensland.
abstract: OBJECTIVE
  To determine the proportion and incidence of calves persistently infected with bovine pestivirus in calves (n = 1521) supplied to the Tick Fever Research Centre and to assess the test regime to detect calves persistently infected with bovine pestivirus.
  
  
  METHODS
  Calves, 1 to 6 weeks old, selected for use in the production of the tick fever vaccine were collected from 21 properties in 56 separate groups between October 1990 and December 1996. Each group was examined for the presence of calves persistently infected with bovine pestivirus.
  
  
  METHODS
  All calves were routinely tested for antibody to bovine pestivirus and bovine pestivirus antigen using a serum neutralisation test and an antigen-capture ELISA, respectively. Pooled lymphocyte samples from calves were also monitored for bovine pestivirus by inoculation of sheep. Whole herd testing was carried out in eight herds, using a serum neutralisation test as a screen test followed by an antigen-capture ELISA of cattle with a serum neutralisation test titre of less than 32.
  
  
  RESULTS
  Fourteen of the 1521 calves tested (0.9%), were detected as persistently infected and the incidence ranged from 0.0 to 3.0% per year over 6 years. Persistently infected calves were found in 13 of the 59 groups and originated from 7 of the 21 herds used. In whole herd testing on the properties of origin, cattle persistently infected with bovine pestivirus were detected in four of the eight herds tested.
  
  
  CONCLUSIONS
  The proportion of calves persistently infected with bovine pestivirus is similar to that in other countries and indicates that bovine pestivirus could be a significant cause of economic loss in Australian cattle herds. In detecting calves persistently infected with bovine pestivirus, the combination of sheep inoculation, paired antigen-capture ELISA and serum neutralisation tests appeared to be highly sensitive and specific.
authors.affiliation: Tick Fever Research Centre, Queensland Department of Primary Industries, Wacol. Bockr@dpi.qld.gov.au
authors.forename: R E
authors.initials: RE
authors.lastname: Bock
authors.forename: B J
authors.initials: BJ
authors.lastname: Rodwell
authors.forename: M
authors.initials: M
authors.lastname: McGowan
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015994:Incidence; D015995:Prevalence; D011793:Queensland / Q000453:epidemiology; D012698:Serologic Tests
chemical_list: D000914:Antibodies, Viral