Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus.
A 10 mM concentration of lithium does not interfere with reverse transcription (RT) or PCR. Sampling of cervicovaginal fluid by vaginal washing, with lithium (10 mM) in the washing buffer as a marker of dilution, may be utilized to accurately determine in HIV-infected women, by quantitative RT-PCR, the genital shedding of acellular HIV RNA at the level of the mucosa itself.
Mohamed
A S
AS
Laboratoire de Virologie, Hôpital Broussais, Paris, France.
Becquart
P
P
Hocini
H
H
Métais
P
P
Kazatchkine
M
M
Bélec
L
L
D002582:Cervix Mucus / Q000821:virology*; D005260:Female; D006678:HIV / Q000302:isolation & purification* / Q000502:physiology; D015658:HIV Infections / Q000503:physiopathology* / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D007507:Therapeutic Irrigation; D017201:Virus Shedding / Q000235:genetics
D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase