title: Effects of botulinum neurotoxin type A on abducens motoneurons in the cat: alterations of the discharge pattern.
abstract: The discharge characteristics that abducens motoneurons exhibit after paralysis of the lateral rectus muscle with botulinum neurotoxin type A were studied in the alert cat. Antidromically identified motoneurons were recorded during both spontaneous and vestibularly induced eye movements. A single injection of 0.3 ng/kg produced a complete paralysis of the lateral rectus muscle lasting for about 12-15 days, whereas after 3 ng/kg the paralysis was still complete at the longest time checked, three months. Motoneurons recorded under the effect of the low dose showed differences in their sensitivities to both eye position and velocity according to the direction of the previous and ongoing movements, respectively. These directional differences could be explained by post-saccadic adaptation of the non-injected eye in the appropriate direction for reducing ocular misalignment. Thus, backward and forward post-saccadic drifts accompanied on- and off-directed saccades, respectively. The magnitude of the drift was similar to the magnitude of changes in eye position sensitivity. The discharge of the high-dose-treated motoneurons could be described in a three-stage sequence. During the initial 10-12 days, motoneuronal discharge resembled the effects of axotomy, particularly in the loss of tonic signals and the presence of exponential-like decay of firing after saccades. In this stage, the conduction velocity of abducens motoneurons was reduced by 21.4%. The second stage was characterized by an overall reduction in firing rate towards a tonic firing at 15-70 spikes/s. Motoneurons remained almost unmodulated for all types of eye movement and thus eye position and velocity sensitivities were significantly reduced. Tonic firing ceased only when the animal became drowsy, but was restored by alerting stimuli. In addition, the inhibition of firing for off-directed saccades was more affected than the burst excitation during on-directed saccades, since in many cells pauses were almost negligible. These alterations could not be explained by adaptational changes in the movement of the non-injected eye. Finally, after 60 days the initial stages of recovery were observed. The present results indicate that the high dose of botulinum neurotoxin produces effects on the motoneuron not attributable to the functional disconnection alone, but to a direct effect of the neurotoxin in the motoneuron and/or its synaptic inputs.
authors.affiliation: Laboratorio de Neurociencia, Facultad de Biología, Universidad de Sevilla, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Moreno-López
authors.forename: R R
authors.initials: RR
authors.lastname: de la Cruz
authors.forename: A M
authors.initials: AM
authors.lastname: Pastor
authors.forename: J M
authors.initials: JM
authors.lastname: Delgado-García
mesh_terms: D000010:Abducens Nerve / Q000187:drug effects*; D000818:Animals; D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D002415:Cats; D005133:Eye Movements / Q000187:drug effects; D009046:Motor Neurons / Q000187:drug effects*; D009498:Neurotoxins / Q000494:pharmacology*
chemical_list: D009498:Neurotoxins; D019274:Botulinum Toxins, Type A