Expression of mRNA for a short form of heparin-binding EGF-like growth factor.
In this paper we report the cloning and characterization of cDNA encoding a novel, short form of heparin-binding EGF-like growth factor (SF HB-EGF), and show expression of specific mRNA in various tissues and cell types. Our data suggest that SF HB-EGF mRNA is a product of alternative splicing. Like normal HB-EGF, SF HB-EGF contains the signal peptide, the propeptide, the heparin-binding domain and the first two conservative disulfide loops of the EGF unit. Instead of the third disulfide loop, the spacer, the transmembrane and the cytoplasmic domains, SF HB-EGF has a nine amino acid tail.
Loukianov
E
E
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo.
Loukianova
T
T
Wiedlocha
A
A
Olsnes
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D004815:Epidermal Growth Factor / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
D004251:DNA Transposable Elements; D018076:DNA, Complementary; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; D004815:Epidermal Growth Factor