The cellular distribution of the adenomatous polyposis coli tumour suppressor protein in neuroblastoma cells is regulated by microtubule dynamics.
The adenomatous polyposis coli tumour suppressor protein is highly expressed in developing rodent brain, but its function is unclear. Recent studies have suggested a role for this protein in regulating microtubule dynamics. Neuro 2A mouse neuroblastoma cells were previously thought not to express this protein. Using immunochemical techniques, this report corrects this observation. Immunoreactive bands of a size consistent with that of the full-length protein were observed by western blotting. Using immunocytochemistry, punctate immunoreactivity localized to areas of the cell containing microtubules, particularly neurite growth cones, in a distribution suggesting a role in neuritogenesis and growth cone extension. The protein did not localize to actin-rich cellular structures, and perturbation of the actin cytoskeleton had no effect upon this distribution. Treatment of cells with taxol to stabilize microtubules caused the concentration of the immunoreactive puncta to the tips of microtubules and areas along the axis of potential microtubule assembly. Treatment of cells with the microtubule disrupting reagent nocodazole showed that over shorter times the punctate distribution was not dependent upon polymerized microtubules. However, at longer incubation times a decrease in punctate immunostaining was observed. These results indicate that the intracellular distribution of the adenomatous polyposis coli protein is dependent upon microtubule but not actin dynamics. A role for this protein in the regulation of directed microtubule assembly is suggested.
Morrison
E E
EE
Molecular Medicine Unit, University of Leeds, St James's University Hospital, U.K.
Askham
J M
JM
Clissold
P
P
Markham
A F
AF
Meredith
D M
DM
D011125:Adenomatous Polyposis Coli / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008870:Microtubules / Q000502:physiology*; D009447:Neuroblastoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000906:Antibodies