Binding of trans-acting factors to the double-stranded variant surface glycoprotein (VSG) expression site promoter of Trypanosoma brucei.
Trypanosoma brucei evades its host's immune response by utilizing the system of antigenic variation, whereby the organism sequentially expresses antigenically distinct variant surface glycoproteins (VSGs). Actively expressed VSG genes are found in VSG expression sites (ESs), and transcription of these ESs is directed by a small promoter composed of two essential cis-acting elements, the VSG ES promoter upstream element (VUE) and VSG ES promoter downstream element (VDE). Using electrophoretic mobility shift assays, we have identified double-stranded DNA binding activity in bloodstream-form trypanosome nuclear extracts. This activity, the VEP complex, is specific for the VSG ES promoter, and requires the intact sequences of the VUE and VDE in the appropriate spacing. These requirements of VEP Complex formation parallel the requirements for promoter function, suggesting that the VEP complex may be composed of functionally significant trans-acting factors. Furthermore, the requirement of both elements suggests that the binding of factors to the promoter may be cooperative. However, subtly different binding characteristics were observed when we used nuclear extracts derived from procyclic trypanosomes.
Pham
V P
VP
Department of Microbiology, Columbia College of Physicians and Surgeons, New York 10032, USA.
Rothman
P B
PB
Gottesdiener
K M
KM
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D005786:Gene Expression Regulation; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics*
D016054:DNA, Protozoan; D004277:DNA, Single-Stranded; D015534:Trans-Activators; D014643:Variant Surface Glycoproteins, Trypanosoma