Effect of milk extract of Semecarpus anacardium nuts on glycohydrolases and lysosomal stability in adjuvant arthritis in rats.
Lysosomal acid hydrolases are thought to play an important role in inflammation associated with rheumatoid arthritis. A Siddha preparation of Semecarpus anacardium nut extract called Serankottai Nei was tested for its capacity to stabilize lysosomes obtained from liver and kidney of adjuvant-induced arthritic animals. Lysosomal membrane stability was measured by determining the release of acid hydrolases from the lysosomes. The drug was administered at a dose level of 150 mg/kg body weight for 14 days to arthritic animals after the adjuvant injection. The total and free activity of lysosomal enzymes were significantly increased in arthritic rats with concomitant increase in plasma levels of protein-bound carbohydrates. Significantly increased lysosomal membrane fragility as observed in arthritic condition was reduced in drug-treated animals. Antiarthritic activity of the drug through its stabilizing action on lysosomal membranes could be inferred from this study.
Vijayalakshmi
T
T
Department of Medical Biochemistry, University of Madras, India.
Muthulakshmi
V
V
Sachdanandam
P
P
D000135:Acid Phosphatase / Q000097:blood; D000284:Administration, Oral; D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000188:drug therapy*; D002402:Cathepsin D / Q000097:blood; D002462:Cell Membrane / Q000187:drug effects; D005966:Glucuronidase / Q000097:blood; D006026:Glycoside Hydrolases / Q000097:blood*; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008892:Milk / Q000737:chemistry; D019158:N-Acetylneuraminic Acid / Q000097:blood; D009754:Nuts / Q000737:chemistry; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D001616:beta-Galactosidase / Q000097:blood
D010936:Plant Extracts; D000135:Acid Phosphatase; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; D005966:Glucuronidase; D002402:Cathepsin D; D019158:N-Acetylneuraminic Acid