title: Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry.
abstract: Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms.
authors.affiliation: Virology Laboratory, Institute of Biology of Nantes University Hospital, France. bmimbert@sante.univ-nantes.fr
authors.forename: B M
authors.initials: BM
authors.lastname: Imbert-Marcille
authors.forename: N
authors.initials: N
authors.lastname: Robillard
authors.forename: A S
authors.initials: AS
authors.lastname: Poirier
authors.forename: M
authors.initials: M
authors.lastname: Coste-Burel
authors.forename: D
authors.initials: D
authors.lastname: Cantarovich
authors.forename: N
authors.initials: N
authors.lastname: Milpied
authors.forename: S
authors.initials: S
authors.lastname: Billaudel
mesh_terms: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000097:blood*; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects; D009504:Neutrophils / Q000821:virology*; D010539:Permeability; D015203:Reproducibility of Results; D013997:Time Factors; D016707:Tissue Fixation
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral