Expression of CD34 and other haematopoietic antigens on neuroblastoma cells: consequences for autologous bone marrow and peripheral blood stem cell transplantation.
Autologous peripheral blood stem cells, obtained by CD34+ stem cell selection, are being used with increasing frequency for transplantation in patients with neuroblastoma. Here, we examined the surface membrane antigens of neuroblastoma cells with a panel of hematopoietic monoclonal antibodies (mAbs), including anti-CD34 mAbs, by flow cytometric analysis. We found stronger binding of anti-CD34 mAbs to clonogenic, less differentiated, non-adherent neuroblastoma cells than to adherent neuroblastoma cells. Moreover, the majority of neuroblastoma cell lines shared hematopoietic-associated antigens with all blood cells. Because of these cross-reactions, especially found with the anti-CD34 mAbs 12.8 and ICH3, we have demonstrated that there is a potential risk of cell harvest contamination by circulating neuroblastoma cells during CD34+ stem cell selection.
Voigt
A
A
Department of Haematology, Oncology and Immunology, Children's Hospital, Friedrich-Schiller-University, Jena, Germany. avoi@kiklil.uni-jena.de
Häfer
R
R
Gruhn
B
B
Zintl
F
F
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D018952:Antigens, CD34 / Q000032:analysis / Q000276:immunology*; D001773:Blood Cells / Q000637:transplantation; D016026:Bone Marrow Transplantation; D003429:Cross Reactions; D000939:Epitopes; D006410:Hematopoiesis / Q000276:immunology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009447:Neuroblastoma / Q000276:immunology* / Q000473:pathology; D033581:Stem Cell Transplantation; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000941:Antigens; D018952:Antigens, CD34; D000939:Epitopes