Isolation and structural characterization of two novel peptides derived from proopiomelanocortin in the pituitary of the rainbow trout.
The trout possesses two POMC genes as a result of duplication of its genome some 25-100 million years ago. One of the POMC molecules exhibits a unique C-terminal extension of 25 amino acid residues which is not found in any other POMC characterized so far. In order to isolate possible novel peptides derived from trout POMC-A, we have raised antibodies against two synthetic epitopes derived from the C-terminal region of the precursor. Two native decapeptides were isolated in pure form from an extract of trout pituitary. The primary structures of these peptides were established as Glu-Gln-Trp-Gly-Arg-Glu-Glu-Gly-Glu-Glu and Ala-Leu-Gly-Glu-Arg-Lys-Tyr-His-Phe-Gln-NH2. The structure of the trout POMC-A cDNA reveals that both peptides are flanked by pairs of basic amino acids or a glycine residue, indicating that they can actually be generated during post-translational processing of POMC-A.
Tollemer
H
H
Laboratory of Cellular and Molecular Neuroendocrinology, University of Rouen, Mont-Saint-Aignan, 76821, France.
Leprince
J
J
Galas
L
L
Vandesande
F
F
Mevel
J C
JC
Tonon
M C
MC
Conlon
J M
JM
Vaudry
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss; D010455:Peptides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D010902:Pituitary Gland / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
D000939:Epitopes; D010455:Peptides; D011333:Pro-Opiomelanocortin