Expression of galanin and nitric oxide synthase in subpopulations of serotonin neurons of the rat dorsal raphe nucleus.
The dorsal raphe nucleus (DR) of the rat was studied with triple labeling immunofluorescence histochemistry to evaluate the quantitative relationships between neurons expressing 5-hydroxytryptamine (5-HT), the neuropeptide galanin (GAL) and the nitric oxide (NO) synthesizing enzyme NO synthase (NOS). In addition retrograde tracing studies were performed. It could be established that a high percentage (between 40 and 60%) of the 5-HT neuron profiles in the ventromedial and dorsomedial subgroups of the DR contained both GAL and NOS after colchicine treatment. This triple coexistence was lower in the dorso-lateral subgroup and much lower in the lateral subgroup (down to 5%). All GAL neuron profiles contained 5-HT, and they constituted up to 80% of all labeled profiles in the rostral ventromedial and caudal dorsomedial subgroups, with the lowest percentage in the lateral subgroup (45%). The percentage of 5-HT-alone neuron profiles in these four subgroups varied between 15-40%. The proportion of 5-HT/NOS neurons was low (a few percent) at all levels, and this was the case also for NOS-alone neuron profiles except in the lateral subgroup, where 10-20% were of this type. It could be established that some 5-HT/GAL/NOS neurons project to the striatum. These retrogradely labeled cells were mainly found in the mid-line subgroups. In the striatum a moderately dense 5-HT fiber network and numerous NOS-positive cell bodies and fibers could be observed. However, only a few, weakly fluorescent GAL fibers were found and in a small number of cases it could be shown or was likely that 5-HT and GAL coexisted. No evidence for coexistence between 5-HT and NOS was obtained. The present findings strongly suggest that a large proportion of the DR 5-HT neurons can synthesize and release two additional messenger molecules, GAL and NO. Furthermore, even if the 5-HT/GAL/NOS neurons project to the striatum, the amounts of GAL and NOS transported to the terminal ramifications in this area are very low. This is in agreement with a very low GAL synthesis in the DR under normal circumstances, which is also indicated by the fact that colchicine treatment is needed to visualize GAL-like immunoreactivity in DR cell bodies. It is possible that NO, and perhaps GAL, in 5-HT neurons exert their main actions at the somatic and dendritic level in the DR.
Xu
Z Q
ZQ
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden. Zhi-Qing.Xu@neuro.ki.se
Hökfelt
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D000818:Animals; D003342:Corpus Striatum / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D019004:Galanin / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D009434:Neural Pathways / Q000737:chemistry; D009474:Neurons / Q000737:chemistry* / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D011903:Raphe Nuclei / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000032:analysis / Q000502:physiology; D013194:Staining and Labeling; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D013266:Stilbamidines; C078371:galanin message-associated peptide; D012701:Serotonin; D019004:Galanin; D019001:Nitric Oxide Synthase