title: Genotypic diversity in field isolates of Babesia bovis from cattle with babesiosis after vaccination.
abstract: OBJECTIVE
  To determine whether particular genotypes of Babesia bovis were common to field isolates obtained from cattle properties in Queensland where the B bovis vaccine had apparently failed.
  
  
  METHODS
  A comparative study of polymerase chain reaction genotypes in different populations of B bovis.
  
  
  METHODS
  Two polymerase chain reaction assays were applied to analyse DNA extracts of B bovis vaccine (K, T and Dixie strains) and 27 field isolates from 24 properties where disease outbreaks had occurred despite the use of the vaccine. To evaluate the stability of the genotypes identified, 11 of the field isolates were inoculated into experimental cattle that had either been previously vaccinated with T strain or not vaccinated.
  
  
  RESULTS
  No particular genotype of B bovis was responsible for the problems observed in previously vaccinated herds. None of the isolates had genotypes identical to the vaccine strains used. No geographic trends among the genotypes were observed. Isolates that originated from the same property also had different genotypes. Blood passage of the 11 field isolates in either previously vaccinated or nonvaccinated cattle did not alter the original genotype.
  
  
  CONCLUSIONS
  No particular genotypes identified by the Bv80 and BvVA1 polymerase chain reaction assays could be associated with vaccine failures.
authors.affiliation: Animal Research Institute, Queensland Department of Primary Industries, Moorooka. LewA@dpi.qld.gov.au
authors.forename: A E
authors.initials: AE
authors.lastname: Lew
authors.forename: R E
authors.initials: RE
authors.lastname: Bock
authors.forename: J M
authors.initials: JM
authors.lastname: Croft
authors.forename: C M
authors.initials: CM
authors.lastname: Minchin
authors.forename: T G
authors.initials: TG
authors.lastname: Kingston
authors.forename: R J
authors.initials: RJ
authors.lastname: Dalgliesh
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D016793:Babesia bovis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D001404:Babesiosis / Q000453:epidemiology / Q000469:parasitology* / Q000517:prevention & control; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000517:prevention & control; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology; D011793:Queensland / Q000453:epidemiology
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D016052:Protozoan Vaccines