title: In vivo study of interferon-alpha-secreting cells in pig foetal lymphohaematopoietic organs following in utero TGEV coronavirus injection.
abstract: Non-infectious UV-inactivated transmissible gastroenteritis virus (TGEV) was previously shown to induce interferon alpha (IFN alpha) secretion following in vitro incubation with blood mononuclear cells. In this study, pig foetuses at different stages of gestation were injected in utero with (a) partially UV-inactivated wild TGEV or (b) fully UV-inactivated wild or dm49-4 mutant TGEV coronavirus. Nucleated cells from foetal liver, bone marrow, spleen and blood were isolated 10 or 20 h after injection and assayed ex vivo for IFN alpha secretion by ELISPOT and ELISA techniques. The administration of TGEV induced IFN alpha-secreting cells in foetal lymphohaematopoietic organs at mid-gestation. In contrast, IFN alpha was not detected in control sham-operated foetuses. A specific point mutation in the amino acid sequence of the viral membrane glycoprotein M of TGEV mutant dm49-4 was associated with lower or absent IFN alpha in utero inducibility by mutant virus as compared with wild virus. Flow cytometry analysis did not show differences in leukocyte surface marker expression between control and TGEV- or between dm49-4 and wild virus-treated foetus cells, with the exception of a reduction in percentages of polymorphonuclear cells in TGEV-treated lymphohaematopoietic tissues, which is probably due to IFN alpha secretion. The present data provided in vivo evidence of IFN alpha secretion at the cell level in foetal lymphohaematopoietic organs. Such IFN alpha-secreting cells in lymphohaematopoietic tissues may be the source of IFN alpha detected during foetal infections.
authors.affiliation: Division of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Nový Hrádek (Czech Republic).
authors.forename: I
authors.initials: I
authors.lastname: Splíchal
authors.forename: Z
authors.initials: Z
authors.lastname: Reháková
authors.forename: M
authors.initials: M
authors.lastname: Sinkora
authors.forename: J
authors.initials: J
authors.lastname: Sinkora
authors.forename: I
authors.initials: I
authors.lastname: Trebichavský
authors.forename: H
authors.initials: H
authors.lastname: Laude
authors.forename: B
authors.initials: B
authors.lastname: Charley
mesh_terms: D000818:Animals; D005260:Female; D005333:Fetus / Q000276:immunology*; D006413:Hematopoietic System / Q000378:metabolism*; D016898:Interferon-alpha / Q000378:metabolism*; D009504:Neutrophils / Q000502:physiology; D011247:Pregnancy; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000502:physiology*
chemical_list: D016898:Interferon-alpha