Antisense sequence-directed cross-linking of RNA oligonucleotides by mitomycin.
Conjugates of mitomycin C (MC) and 15-mer oligodeoxyribonucleotides (ODNs) were synthesized in which the 7-amino group of MC was tethered by either a (-CH2-)6 or a (-CH2-)12 linker to the 5'-terminal phosphate of the ODNs. The conjugates were shown to be cross-linked selectively to complementary 18-mer oligoribonucleotides (ORNs). The cross-linking was dependent on reductive activation of the MC moiety of the conjugates by NADPH-cytochrome c reductase/NADPH. The cross-linked ODN-ORN hybrid duplexes were characterized as such by degeneration by RNase H. Cross-linking efficiencies of the conjugates were 50 and 25% in the case of the (-CH2-)12 tether and the (-CH2-)6 tether respectively The results demonstrate the feasibility of sequence-targeted alkylation of RNA by MC via antisense recognition.
Maruenda
H
H
Department of Chemistry, Hunter College, The City University of New York, NY 10021, USA. hmaruen@pucp.edu.pe
Tomasz
M
M
D018906:Antineoplastic Agents, Alkylating / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D016685:Mitomycin / Q000737:chemistry*; D016372:RNA, Antisense / Q000737:chemistry*
D018906:Antineoplastic Agents, Alkylating; D003432:Cross-Linking Reagents; D016372:RNA, Antisense; D016685:Mitomycin