Langerhans cell tropism of human immunodeficiency virus type 1 subtype A through F isolates derived from different transmission groups.
To test the hypothesis that some subtypes of human immunodeficiency virus type 1 (HIV-1), especially subtype E, are more likely to infect mature Langerhans cells (mLC), we titrated a panel of 26 primary HIV-1 isolates of subtypes A through F on peripheral blood mononuclear cells (PBMC) and mLC. The majority of HIV-1 isolates from heterosexually infected patients did not show a preferred tropism for mLC compared to homosexually transmitted HIV-1 isolates. Only 6 of 26 isolates, 2 from patients infected by homosexual contact and 4 from patients infected by heterosexual contact, showed a higher infectivity for mLC than for PBMC. Both syncytium-inducing and non-syncytium-inducing isolates were able to infect mLC which express mRNA for the chemokine receptors CCR3, CCR5, and CXCR4.
Dittmar
M T
MT
Chester Beatty Laboratories, Institute of Cancer Research, London, United Kingdom.
Simmons
G
G
Hibbitts
S
S
O'Hare
M
M
Louisirirotchanakul
S
S
Beddows
S
S
Weber
J
J
Clapham
P R
PR
Weiss
R A
RA
D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology* / Q000635:transmission*; D002478:Cells, Cultured; D005260:Female; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000502:physiology*; D018451:Homosexuality, Male; D006801:Humans; D007801:Langerhans Cells / Q000821:virology*; D007962:Leukocytes / Q000821:virology*; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D010641:Phenotype; D012307:Risk Factors; D012725:Sexual Behavior; D012867:Skin / Q000166:cytology / Q000821:virology