Gene transfer using replication-defective human foamy virus vectors.
Replication-defective vectors based on an infectious molecular clone of human foamy virus (HFV) were constructed by deletion and replacement of the accessory genes with expression cassettes for puromycin-resistance and beta-glucouronidase. Cell lines which produced in excess of 10(5) helper virus-free transducing units/ml were generated by trans-complementation of the replication defect using a BHK-21-derived cell line expressing the Bel-1 transactivator. Vectors based on the HFV genome may provide useful alternatives to existing retroviral vectors.
Bieniasz
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PD
Department of G. U. Medicine and Communicable Diseases, Imperial College School of Medicine at St. Mary's, Paddington, London, United Kingdom.
Erlwein
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O
Aguzzi
A
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Rethwilm
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A
McClure
M O
MO
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D003673:Defective Viruses; D004352:Drug Resistance, Microbial; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000096:biosynthesis; D006801:Humans; D007668:Kidney; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012191:Retroviridae Proteins / Q000096:biosynthesis*; D016092:Spumavirus; D015534:Trans-Activators / Q000096:biosynthesis*; D014162:Transfection / Q000379:methods; D014779:Virus Replication
D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012191:Retroviridae Proteins; D015534:Trans-Activators; C074061:bel1 protein, Human foamy virus; D005966:Glucuronidase