[Approaches to selecting a strain of hantavirus for developing an inactivated brain vaccine].
Four strains isolated from patients with hemorrhagic fever with the renal syndrome and from field mice, natural hosts of Hantavirus, were examined in order to choose a functionally active strain fit for the development of inactivated Hantavirus brain vaccine. Virulent strain PM-10508/89 isolated from mouse lungs was chosen. The strain was highly virulent for newborn mice and rats: 100% of infected mice aged up to 24 h and 75% of rats developed an acute lethal infection upon challenge. The incubation period of Hantavirus adapted to mouse brain was decreased from 19 to 7 days. The infective titer of the virus in brain suspension was 0.1 to 6.5 lg LD50/ml. The strain was highly immunogenic and possessed a high hemagglutinating activity.
Astakhova
T I
TI
Slonova
R A
RA
Kompanets
G G
GG
D000818:Animals; D001921:Brain / Q000821:virology*; D006476:Orthohantavirus / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D018778:Hantavirus Infections / Q000517:prevention & control / Q000821:virology; D006387:Hemagglutination, Viral; D006801:Humans; D051379:Mice; D051381:Rats; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D014774:Virulence
D015164:Vaccines, Inactivated; D014765:Viral Vaccines