title: [Comparative analysis of expression of genes for 2',5'-oligoadenylate synthetase and mRNA for dsRNA-protein kinase in human fibroblasts infected with the Sindbis alphavirus and induced by alpha-interferon].
abstract: Expression of 2,5-OAS and ds-protein kinase (ds-PK) in human fibroblast culture following treatment with alpha-IF in a dose of 1000 U or challenge with Karelian fever virus (KFV) (5 PFU/cell, 4 h.p.i.) was compared. A highly sensitive and rapid method, coupled RT-PCR, was used to detect mRNA transcription levels. Alpha-IF had an expressed stimulating effect on 2,5-OAS mRNA level, whereas the virus markedly inhibited the synthesis of ds-PK mRNA.
authors.forename: T M
authors.initials: TM
authors.lastname: Sokolova
authors.forename: O V
authors.initials: OV
authors.lastname: Bobrova
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Lebedev
authors.forename: N S
authors.initials: NS
authors.lastname: Bystrov
authors.forename: L V
authors.initials: LV
authors.lastname: Uryvaev
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000821:virology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012845:Sindbis Virus / Q000502:physiology*; D014158:Transcription, Genetic; D019892:eIF-2 Kinase
chemical_list: D016898:Interferon-alpha; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; D019892:eIF-2 Kinase; D015088:2',5'-Oligoadenylate Synthetase