Protein exudation induced by manipulation of the intestines and mesentery during laparotomy in rat. A study of the mechanism of 'third space' loss.
BACKGROUND-MATERIALS: Much extracellular fluid administration is required during major surgery because of the functional loss of extracellular fluid. In the present study we investigated the effect of manipulation of the intestines and mesentery in a rat laparotomy model. METHODS-RESULTS-CONCLUSIONS: The results showed that manipulation significantly increased the amount of protein exudated into the peritoneal space and induced a marked histopathological inflammatory response including polymorphonuclear leukocyte infiltration and mast cell degradation. These findings suggest that the inflammatory response induced by manipulation may, at least in part, explain the underlying mechanism of 'third space' loss during abdominal intestinal surgery.
Moriwaki
K
K
Department of Anesthesiology and Critical Care Medicine, School of Medicine, Hiroshima University, Japan. moriwak@ipc.hiroshima-u.acjp
Fujii
K
K
Yuge
O
O
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D015550:Cell Degranulation / Q000502:physiology; D005122:Exudates and Transudates / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000473:pathology / Q000601:surgery; D007813:Laparotomy; D008297:Male; D008407:Mast Cells / Q000502:physiology; D008643:Mesentery / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D009504:Neutrophils / Q000502:physiology; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar
D001798:Blood Proteins