Rapid pharmacokinetic screening of salbutamol in plasma samples by column-switching high-performance liquid chromatography-electrospray mass spectrometry.
In order to obtain pharmacokinetic data from studies in humans, a sensitive and selective assay for the quantification of salbutamol in human plasma samples was required. This report describes an automated high-performance liquid chromatography-mass spectrometry assay with pre-column enrichment using internal standard calibration for the quantification of salbutamol and the validation of the assay. The lower limit of quantitation is 0.2 ng/ml with an accuracy and imprecision of less than 7%. The analysis time is 8 min per sample.
Schmeer
K
K
Department of Pharmacokinetics and Metabolism, Dr. Karl Thomae GmbH, Riss, Germany.
Sauter
T
T
Schmid
J
J
D000318:Adrenergic beta-Agonists / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D000420:Albuterol / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014316:Tritium
D000318:Adrenergic beta-Agonists; D014316:Tritium; D000420:Albuterol