Quantification of bovine albumin fraction V in cellular antigens of antileptospirosical Cuban vaccine vaxSpiral.
A solid phase immunoradiometric assay (IRMA) for quantification of bovine albumin fraction V (Bov.Alb.FV) in antileptospirosical Cuban vaccine vaxSpiral is described in the present work. Anti-Bov.Alb.FV IgG raised against rabbit purified by affinity chromatography was used as first antibody. Anti-rabbit IgG labeled by Chloromine-T reaction was used as a tracer and the method has demonstrated to be sensitive with high intra- and inter-assay reproducibility. Eight lots of vaccinal antigens were evaluated and in all of the cases, the bovine albumin fraction V concentration was lower than 1 microgram/mL, as the World Health Organization (WHO) establishes. This IRMA is a simple and sensitive assay and could be used as control method for all human vaccines that use Bov.Alb.FV in their production process, even cellular vaccines.
Valdés González
T
T
Finlay Institute, La Habana, Cuba.
González Pérez
M
M
González González
M
M
Cabrera Arias
R A
RA
Muñoz Carnago
E
E
Lastre González
M
M
Sierra González
G
G
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001428:Bacterial Vaccines / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D015592:Immunoradiometric Assay; D007919:Leptospira / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000032:analysis*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine