Failure to detect Chlamydia trachomatis in cell culture by using a monoclonal antibody directed against the major outer membrane protein.
Two commercially available monoclonal antibodies for cell culture confirmation of Chlamydia trachomatis were compared in two prospective studies and one large retrospective study. In total, more than 33,000 genital specimens were cultured in parallel and stained with both antibodies, one of which was directed against the major outer membrane protein (MOMP) and one of which was directed against the lipopolysaccharide (LPS). We found the anti-LPS-based assay to be more sensitive and as specific as the anti-MOMP-based assay for C. trachomatis cell culture confirmation of genital specimens.
Boman
J
J
Department of Clinical Virology, The University Hospital of Umeå, Sweden. jens.boman@climi.umu.se
Gaydos
C
C
Juto
P
P
Wadell
G
G
Quinn
T C
TC
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000276:immunology*; D005188:False Negative Reactions; D052776:Female Urogenital Diseases / Q000175:diagnosis*; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D052801:Male Urogenital Diseases; D018272:Porins; D011446:Prospective Studies; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis