Desmin is essential for the tensile strength and integrity of myofibrils but not for myogenic commitment, differentiation, and fusion of skeletal muscle.
A null mutation was introduced into the mouse desmin gene by homologous recombination. The desmin knockout mice (Des -/-) develop normally and are fertile. However, defects were observed after birth in skeletal, smooth, and cardiac muscles (Li, Z., E. Colucci-Guyon, M. Pincon-Raymond, M. Mericskay, S. Pournin, D. Paulin, and C. Babinet. 1996. Dev. Biol. 175:362-366; Milner, D.J., G. Weitzer, D. Tran, A. Bradley, and Y. Capetanaki. 1996. J. Cell Biol. 134:1255- 1270). In the present study we have carried out a detailed analysis of somitogenesis, muscle formation, maturation, degeneration, and regeneration in Des -/- mice. Our results demonstrate that all early stages of muscle differentiation and cell fusion occur normally. However, after birth, modifications were observed essentially in weight-bearing muscles such as the soleus or continually used muscles such as the diaphragm and the heart. In the absence of desmin, mice were weaker and fatigued more easily. The lack of desmin renders these fibers more susceptible to damage during contraction. We observed a process of degeneration of myofibers, accompanied by macrophage infiltration, and followed by a process of regeneration. These cycles of degeneration and regeneration resulted in a relative increase in slow myosin heavy chain (MHC) and decrease in fast MHC. Interestingly, this second wave of myofibrillogenesis during regeneration was often aberrant and showed signs of disorganization. Subsarcolemmal accumulation of mitochondria were also observed in these muscles. The lack of desmin was not compensated by an upregulation of vimentin in these mice either during development or regeneration. Absence of desmin filaments within the sarcomere does not interfere with primary muscle formation or regeneration. However, myofibrillogenesis in regenerating fibers is often abortive, indicating that desmin may be implicated in this repair process. The results presented here show that desmin is essential to maintain the structural integrity of highly solicited skeletal muscle.
Li
Z
Z
Station Centrale de Microscopie Electronique, Institut Pasteur, Paris, France.
Mericskay
M
M
Agbulut
O
O
Butler-Browne
G
G
Carlsson
L
L
Thornell
L E
LE
Babinet
C
C
Paulin
D
D
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002459:Cell Fusion / Q000187:drug effects / Q000235:genetics; D004179:Cobra Cardiotoxin Proteins / Q000008:administration & dosage; D003893:Desmin / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D004594:Electrophysiology; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000235:genetics; D017353:Gene Deletion; D007273:Injections, Intramuscular; D051379:Mice; D018345:Mice, Knockout; D009043:Motor Activity / Q000235:genetics; D009119:Muscle Contraction / Q000235:genetics; D018485:Muscle Fibers, Skeletal / Q000145:classification / Q000201:enzymology; D018908:Muscle Weakness / Q000235:genetics; D018482:Muscle, Skeletal / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D009210:Myofibrils / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000378:metabolism / Q000502:physiology; D010805:Physical Conditioning, Animal; D012038:Regeneration / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D019170:Somites / Q000502:physiology; D014746:Vimentin / Q000502:physiology
D004179:Cobra Cardiotoxin Proteins; D003893:Desmin; D014746:Vimentin; D000251:Adenosine Triphosphatases; D018995:Myosin Heavy Chains