Inhibition of adhesion molecules by budesonide on a human epithelial cell line (lung carcinoma).
Inhaled corticosteroids in the treatment of asthma have been shown to produce marked reductions in the number of inflammatory cells (mainly mast cells and eosinophils) and their products at bronchial level (such as cytokines). Recently, it has been demonstrated that epithelial cells express ICAM-1/CD54 in allergic patients both during natural allergen exposure and after allergen challenge. We have previously demonstrated that deflazacort (a systemic steroid) reduces the expression of ICAM-1 on conjunctival epithelial cells. The present study aimed to evaluate the effects exerted by budesonide on adhesion molecule expression by a human epithelial cell line (lung carcinoma: DM) and on soluble ICAM-1. Budesonide was added at concentrations corresponding to 10(-8), 10(-7), and 10(-6) mol/l in cultured epithelial cells, either in the absence of any stimulus or in the presence of interferon-gamma (IFN-gamma) at 500 U/ml. After 24 h of incubation, cytofluorometric analysis was performed for ICAM-1 and CD29/VLA beta 1. The 24-h supernatants of the same cultures were collected and then evaluated for soluble ICAM-1 (sICAM-1). The results showed that budesonide inhibits ICAM-1 and CD29 basal expression on the cells studied (P < 0.05): budesonide was effective in a dose-dependent manner. In addition, budesonide reduced surface ICAM-1 upregulation induced by IFN-gamma at 500 U/ml (P < 0.05). Finally, cell cultures with budesonide showed decreased levels of soluble ICAM-1 in basal condition, but not after IFN-gamma stimulation.
Paolieri
F
F
Dipartimento di Medicine Interna, University of Genoa, Italy.
Battifora
M
M
Riccio
A M
AM
Ciprandi
G
G
Scordamaglia
A
A
Morelli
C
C
Bagnasco
M
M
Canonica
G W
GW
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy / Q000276:immunology; D001993:Bronchodilator Agents / Q000494:pharmacology*; D019819:Budesonide / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell; D004353:Drug Evaluation, Preclinical; D006801:Humans; D019012:Integrin beta1 / Q000187:drug effects*; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects*; D007371:Interferon-gamma / Q000276:immunology*; D008175:Lung Neoplasms; D014407:Tumor Cells, Cultured
D000893:Anti-Inflammatory Agents; D001993:Bronchodilator Agents; D019012:Integrin beta1; D018799:Intercellular Adhesion Molecule-1; D019819:Budesonide; D007371:Interferon-gamma