Rapid evolution of a young L1 (LINE-1) clade in recently speciated Rattus taxa.
L1 elements are retrotransposons that have been replicating and evolving in mammalian genomes since before the mammalian radiation. Rattus norvegicus shares the young L1mlvi2 clade only with its sister taxon, Rattus cf moluccarius. Here we compared the L1mlvi2 clade in these recently diverged species and found that it evolved rapidly into closely related but distinct clades: the L1mlvi2-rm clade (or subfamily), characterized here from R. cf moluccarius, and the L1mlvi2-rn clade, originally described in R. norvegicus. In addition to other differences, these clades are distinguished by a cluster of amino acid replacement substitutions in ORF I. Both rat species contain the L1mlvi2-rm clade, but the L1mlvi2-rn clade is restricted to R. norvegicus. Therefore, the L1mlvi2-rm clade arose prior to the divergence of R. norvegicus and R. cf moluccarius, and the L1mlvi2-rn clade amplified after their divergence. The total number of L1mlvi2-rm elements in R. cf moluccarius is about the same as the sum of the L1mlvi2-rm and L1mlvi2-rn elements in R. norvegicus. The possibility that L1 amplification is in some way limited so that the two clades compete for replicative supremacy as well as the implications of the other distinguishing characteristic of the L1mlvi2-rn and L1mlvi2-rm clades are discussed.
Cabot
E L
EL
Section on Genomic Structure and Function, NIDDK, NIH, Bethesda, MD 20892-0830, USA. cabot@gcg.com
Angeletti
B
B
Usdin
K
K
Furano
A V
AV
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D005784:Gene Amplification; D008969:Molecular Sequence Data; D009115:Muridae / Q000145:classification / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D051381:Rats / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013997:Time Factors
D017931:DNA Primers; D018626:Retroelements; D004247:DNA