title: Structure and mobility of the PUT3 dimer.
abstract: The solution structure and backbone dynamics of the transcriptional activator PUT3 (31-100) has been characterized using NMR spectroscopy. PUT3 (31-100) contains three distinct domains: a cysteine zinc cluster, linker, and dimerization domain. The cysteine zinc cluster of PUT3 closely resembles the solution structure of GAL4, while the dimerization domain forms a long coiled-coil similar to that observed in the crystal structures of GAL4 and PPR1. However, the residues at the N-terminal end of the coiled-coil behave very differently in each of these proteins. A comparison of the structural elements within this region provides a model for the DNA binding specificity of these proteins. Furthermore, we have characterized the dynamics of PUT3 to find that the zinc cluster and dimerization domains have very diverse dynamics in solution. The dimerization domain behaves as a large protein, while the peripheral cysteine zinc clusters have dynamic properties similar to small proteins.
authors.affiliation: Committee on Higher Degrees in Biophysics, Harvard University, Cambridge, Massachusetts 02138, USA.
authors.forename: K J
authors.initials: KJ
authors.lastname: Walters
authors.forename: K T
authors.initials: KT
authors.lastname: Dayie
authors.forename: R J
authors.initials: RJ
authors.lastname: Reece
authors.forename: M
authors.initials: M
authors.lastname: Ptashne
authors.forename: G
authors.initials: G
authors.lastname: Wagner
mesh_terms: D000595:Amino Acid Sequence; D003545:Cysteine / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D019281:Dimerization; D005656:Fungal Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000737:chemistry*; D014157:Transcription Factors; D015032:Zinc / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; C068399:PUT3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D004247:DNA; D015032:Zinc; D003545:Cysteine