Regulation of cell cycle-related genes in rat hepatocytes by transforming growth factor beta1.
Transforming growth factor beta (TGF-beta) is a potent inhibitor of the proliferation of many cell types. We investigated the effects of TGF-beta1 on cyclin D1, cyclin A, p21, p27, and p53 mRNA expressions in primary cultured rat hepatocytes by the reverse-transcription polymerase chain reaction (RT-PCR) method. TGF-beta1 decreased the level of cyclin A mRNA in a dose-dependent manner, while it had little effect on the level of cyclin D1 mRNA. p21 mRNA expression was greatly induced by TGF-beta1 in a p53-independent mechanism, while p27 mRNA expression was not affected by TGF-beta1. These results suggest that TGF-beta1 may inhibit liver cell proliferation by regulating p21 and cyclin A mRNAs.
Sugiyama
A
A
First Department of Internal Medicine, Gifu University School of Medicine, Gifu, 500, Japan.
Nagaki
M
M
Shidoji
Y
Y
Moriwaki
H
H
Muto
Y
Y
D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D018797:Cell Cycle Proteins; D016212:Transforming Growth Factor beta