Brain-derived neurotrophic factor in human platelets.
A sensitive, capture enzyme-linked immunosorbent assay (CELISA) has been applied to the accurate and reproducible measurement of brain-derived neurotrophic factor (BDNF) protein in normal human blood platelets, a mean concentration of 1.03 +/- 0.04 ng (SEM)/mg of platelet protein being observed. The method, which requires only 10 ml blood, is now suitable for the investigation of a variety of clinical disorders.
Pliego-Rivero
F B
FB
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
Bayatti
N
N
Giannakoulopoulos
X
X
Glover
V
V
Bradford
H F
HF
Stern
G
G
Sandler
M
M
D001792:Blood Platelets / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans
D019208:Brain-Derived Neurotrophic Factor