Spectrophotometric determination of the positional specificity of nonspecific and 1,3-specific lipases.
Using commercially available thiosubstrates, such as 2,3-dimercapto-1-propanol tributyrate, the regio-specificities of 1,3-specific and nonspecific lipases was confirmed. The spectrophotometric test is a simple, rapid, and convenient alternative method to those previously reported for the characterization of the positional specificities of new lipases.
Farias
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Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (CONICET-UNT), San Miguel de Tucumán, Argentina. farias@insibio.unt.edu.ar
Torres
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Canela
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D001230:Aspergillus / Q000201:enzymology; D004112:Dimercaprol / Q000031:analogs & derivatives / Q000737:chemistry; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology; D013053:Spectrophotometry / Q000379:methods*; D013379:Substrate Specificity
D004112:Dimercaprol; C014496:2,3-dimercaptopropan-1-ol tributyrate; D008049:Lipase