title: Monoclonal antibody E8-18 identifies an integral membrane surface protein unique to Mycoplasma capricolum subsp. capripneumoniae.
abstract: Monoclonal antibody (MAb) E8-18 reacted with four isolates of Mycoplasma capricolum subsp. capripneumoniae in Western blots identifying an epitope on a 24 kDa antigen (p24). MAb E8-18 did not react with 11 isolates belonging to four other Mycoplasma species or subspecies closely related to M. capricolum subsp. capripneumoniae. A combination of trypsin treatment of intact organisms and detergent-phase partitioning revealed p24 to be an integral M. capricolum subsp. capripneumoniae surface membrane protein.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.
authors.forename: F R
authors.initials: FR
authors.lastname: Rurangirwa
authors.forename: P S
authors.initials: PS
authors.lastname: Shompole
authors.forename: A N
authors.initials: AN
authors.lastname: Wambugu
authors.forename: S M
authors.initials: SM
authors.lastname: Kihara
authors.forename: T C
authors.initials: TC
authors.lastname: McGuire
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D009174:Mycoplasma / Q000145:classification / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D008565:Membrane Proteins; C489067:Nrsn1 protein, mouse