Importance of the galE gene on the virulence of Pasteurella multocida.
The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduced amino acid sequence displayed the highest identity (85%) to the GalE protein of Haemophilus influenzae. However, the gene organization surrounding the galE locus was different from that of H. influenzae. A galE-defective mutant of P. multocida was obtained by replacement of the active galE gene by a copy inactivated in vitro. The resulting galE mutant was highly attenuated as seen in a biological test carried out in a mouse model.
Fernández de Henestrosa
A R
AR
Department of Genetics and Microbiology, Universitat Autónoma de Barcelona, Bellaterra, Spain.
Badiola
I
I
Saco
M
M
Perez de Rozas
A M
AM
Campoy
S
S
Barbé
J
J
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D016979:Pasteurella multocida / Q000235:genetics* / Q000472:pathogenicity; D014534:UDPglucose 4-Epimerase / Q000235:genetics*; D014774:Virulence
D014534:UDPglucose 4-Epimerase; C020551:galactose epimerase