NMR assignment of the galactomannan of Candida lipolytica.
The chemical structure of the cell wall galactomannan of Candida lipolytica was analyzed using two-dimensional NMR techniques without chemical fragmentation. The H-1-H-2-correlated cross-peaks of the galactomannan indicated that it consists of an alpha-1,6-linked mannan backbone moiety with side chains. A sequential NMR assignment of the side chains through nuclear Overhauser effect (NOE) cross-peaks indicated that the triose side chain contains an alpha-1,2-linked galactopyranose unit at the non-reducing terminal. The structure was significantly different from the galactomannan of Trichophyton. The molar ratio of the side chains calculated from the H-1 signal dimensions indicated that ca. 45% of the backbone alpha-1,6-linked mannose units are not substituted with side chains and are responsible for the reactivity of the galactomannan with factor 9 serum.
Ikuta
K
K
Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Sendai, Miyagi, Japan.
Shibata
N
N
Kanehiko
H
H
Kobayashi
H
H
Suzuki
S
S
Okawa
Y
Y
D002175:Candida / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D005690:Galactose / Q000031:analogs & derivatives; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008358:Mannose; D008969:Molecular Sequence Data; D013237:Stereoisomerism; D014249:Trichophyton / Q000737:chemistry
D008351:Mannans; C012990:galactomannan; D008358:Mannose; D005690:Galactose