Laboratory and field comparisons of adenosine influx in Plasmodium falciparum and Plasmodium vivax infected erythrocytes with genetic abnormalities from patients in Myanmar.
Influx of the purine nucleoside, adenosine, was assessed in erythrocytes from both normal subjects and from subjects with a range of genetically determined erythrocyte disorders from Myanmar. The latter included alpha-thalassemia major (Myanmar variant), beta-thalassemia major (Myanmar variant), beta-thalassemia trait, HbEE and HbAE erythrocytes and two variants of glucose-6-phosphate dehydrogenase (G6PDH) deficiency. Significant reductions (p < 0.01) of adenosine influx were observed in erythrocytes from individuals with alpha- and beta-thalassemia major and severe G6PDH deficiency. Abnormal erythrocytes infected with the malarial parasites, Plasmodium falciparum or Plasmodium vivax, demonstrated a reduction in adenosine transport which correlated with the proportion of abnormal erythrocytes present in the samples obtained. The effect of nitrobenzylthioinosine (NBMPR) on adenosine influx was explored in normal and abnormal erythrocytes. In all these cases, NBMPR completely inhibited the transport of adenosine. However, transport of adenosine into P. falciparum and P. vivax-infected normal erythrocytes and abnormal cells was only inhibited 50-60% by NBMPR. The combination of tubercidin and NBMPR completely blocked adenosine transport into both normal and abnormal erythrocytes infected with either P. falciparum or P. vivax.
Myint-Oo
Department of Medical Research, Ministry of Health, Dagon PO, Myanmar, Australia.
O'Sullivan
W J
WJ
Gero
A M
AM
D000241:Adenosine / Q000097:blood*; D000328:Adult; D000345:Affinity Labels / Q000494:pharmacology; D002648:Child; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D004913:Erythrocytes, Abnormal / Q000378:metabolism / Q000469:parasitology*; D005260:Female; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000378:metabolism / Q000469:parasitology*; D006453:Hemoglobinopathies / Q000097:blood / Q000235:genetics*; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood*; D016780:Malaria, Vivax / Q000097:blood*; D008297:Male; D002052:Myanmar; D013868:Thioinosine / Q000031:analogs & derivatives / Q000494:pharmacology; D014372:Tubercidin / Q000494:pharmacology
D000345:Affinity Labels; D013868:Thioinosine; C001789:4-nitrobenzylthioinosine; D000241:Adenosine; D014372:Tubercidin