title: Conformational analysis of LYS(11-36), a peptide derived from the beta-sheet region of T4 lysozyme, in TFE and SDS.
abstract: The solution conformation of a peptide LYS(11-36), which corresponds to the beta-sheet region in T4 lysozyme, has been examined in aqueous solution, TFE, and SDS micelles by CD and 1H NMR spectroscopy. Secondary structure predictions suggest some beta-sheet and turn character in aqueous solution but predict a helical conformation in a more hydrophobic environment. The predictions were supported by the CD and NMR studies which showed the peptide to be relatively unstructured in aqueous solution, although there was some evidence of a beta-turn conformer which was maintained in 200 mM SDS and, to a lesser extent, in 50% TFE. The peptide was significantly helical in the presence of either 50% TFE or 200 mM SDS. TFE and SDS titrations showed that the peptide could form helical, sheet, or extended structure depending on the TFE or SDS concentration. The studies indicate that peptide environment is the determining factor in secondary structure adopted by LYS(11-36).
authors.affiliation: Victorian College of Pharmacy, Monash University, 381 Royal Pde, Parkville 3052, Australia.
authors.forename: L V
authors.initials: LV
authors.lastname: Najbar
authors.forename: D J
authors.initials: DJ
authors.lastname: Craik
authors.forename: J D
authors.initials: JD
authors.lastname: Wade
authors.forename: D
authors.initials: D
authors.lastname: Salvatore
authors.forename: M J
authors.initials: MJ
authors.lastname: McLeish
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009113:Muramidase; D010446:Peptide Fragments / Q000138:chemical synthesis; D017433:Protein Structure, Secondary; D011522:Protons; D012967:Sodium Dodecyl Sulfate; D012996:Solutions; D014270:Trifluoroethanol
chemical_list: D010446:Peptide Fragments; D011522:Protons; D012996:Solutions; C108356:T4 lysozyme (11-36); D012967:Sodium Dodecyl Sulfate; D014270:Trifluoroethanol; D009113:Muramidase