Water deprivation increases the expression of pituitary adenylate cyclase-activating polypeptide gene in the rat subfornical organ.
The effect of water deprivation on the expression of pituitary adenylate cyclase-activating polypeptide (PACAP) was examined in the rat subfornical organ (SFO), using a combination of immunohistochemistry and in situ hybridization histochemistry. In the euhydrated condition, PACAP-immunoreactivity (PACAP-IR) and the expression of PACAP gene was observed in the SFO. Water deprivation for 24 h and 48 h caused a significant increase in PACAP gene transcripts in the SFO, compared with euhydrated animals. Additionally, water deprivation for 48 h caused an increase in PACAP-IR. This increase of PACAP-IR was demonstrated in both nerve fibers and cell bodies. High correlation was found between the localization of PACAP-IR cell bodies and PACAP messenger RNA synthesizing cell bodies in the peripheral part of the SFO. These results suggest that PACAP in the SFO may play a role in the humoral and neural changes associated with the regulation of body fluid balance after water deprivation.
Nomura
M
M
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Ueta
Y
Y
Larsen
P J
PJ
Hannibal
J
J
Serino
R
R
Kabashima
N
N
Shibuya
I
I
Yamashita
H
H
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000502:physiology; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009479:Neuropeptides / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics*; D009994:Osmolar Concentration; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000097:blood; D013356:Subfornical Organ / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D014868:Water Deprivation / Q000502:physiology*
C495403:Adcyap1 protein, rat; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012964:Sodium