title: Insect mucin-type glycoprotein: immunodetection of the O-glycosylated epitope in Drosophila melanogaster cells and tissues.
abstract: A mucin-type glycoprotein (GP) from cultured embryonic cells of Drosophila melanogaster was isolated and used to raise monoclonal antibodies (MAbs). Epitope(s) recognized by MAbs were sensitive to the treatment by O-glycanase, which specifically cleaves off O-linked mucin-type Gal(beta 1,3)GalNAc disaccharide, representing the major part of the carbohydrate moiety of Drosophila GP. Using high-affinity MAbs against carbohydrate epitopes of the Drosophila mucin GP we demonstrated its accumulation in culture medium, as well as in cultured cells, which proved to be regulated by 20-hydroxyecdysone. Mucin GPs carrying Gal(beta 1,3)GalNAc disaccharide recognized by the MAbs were immunochemically localized in several Drosophila tissues of ectodermal, mesodermal and germ line origin, including epidermal and follicle cells capable of their secretion.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia. dmga@glas.apc.org
authors.forename: A A
authors.initials: AA
authors.lastname: Kramerov
authors.forename: E A
authors.initials: EA
authors.lastname: Mikhaleva
authors.forename: M
authors.initials: M
authors.lastname: Rozovsky Ya
authors.forename: T V
authors.initials: TV
authors.lastname: Pochechueva
authors.forename: N A
authors.initials: NA
authors.lastname: Baikova
authors.forename: E L
authors.initials: EL
authors.lastname: Arsenjeva
authors.forename: V A
authors.initials: VA
authors.lastname: Gvozdev
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000196:embryology; D000939:Epitopes / Q000032:analysis*; D006031:Glycosylation; D009077:Mucins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D009077:Mucins