Clinical utility of heteroduplex analysis of TCR gamma gene rearrangements in the diagnosis of T-cell lymphoproliferative disorders.
We conducted this study to assess the clinical utility of heteroduplex analysis of polymerase chain reaction (PCR) products for Tgamma gene rearrangements in the diagnosis of T-cell lymphoproliferative disorders (LPDs) using a single set of consensus primers. This method was used to study 103 cases using fresh or frozen tissue. These included 62 cases of T-cell LPDs, 25 cases of B-cell LPDs, 3 cases of anaplastic large cell lymphomas of undefined lineage, and 13 cases of reactive disorders. The latter group served as the negative control group. For all cases of T-cell LPDs, the results of Tbeta Southern analysis (SA) and heteroduplex PCR analysis were compared. We also studied 10 cases of postthymic T-cell lymphoma using paraffin-embedded tissue. Heteroduplex Tgamma PCR was positive in 51 (89%) of 57 cases that were rearranged by Tbeta SA. Eight of the 10 cases of T-cell lymphoma analyzed using paraffin-embedded tissue were positive. There were no false-positive results. These results demonstrate that this method is an effective screening test in the clinical laboratory. A positive result obviates the need to perform SA. Preliminary results suggest this method is useful using paraffin-embedded tissue.
Chhanabhai
M
M
Division of Laboratory Medicine, British Columbia Cancer Agency and the University of British Columbia, Vancouver, Canada.
Adomat
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SA
Gascoyne
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Horsman
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D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D005434:Flow Cytometry; D005784:Gene Amplification; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / Q000235:genetics*; D006801:Humans; D016399:Lymphoma, T-Cell / Q000737:chemistry / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D010338:Pathology, Clinical / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D013601:T-Lymphocytes / Q000737:chemistry* / Q000473:pathology
D017931:DNA Primers; D009692:Nucleic Acid Heteroduplexes; D004247:DNA