Conditioned immunosuppression of lipopolysaccharide-induced antibody response of orally immunized mice.
BALB/C mice previously conditioned in a paired regimen in which cycophosphamide was given in association with a novel taste in the drinking water (saccharin, SAC) subsequently received LPS (1mg/mouse) by oral gavage. Subgroups of mice were reexposed to SAC or water respectively, and anti-LPS antibody production measured 6 days later using an ELISA/ELISPOT assay. Our data show that the immune response to this T-independent antigen, administered orally, is amenable to conditioned immunosuppression in this model system.
Morato
E F
EF
Department of Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Brazil.
Gerbase-Delima
M
M
Gorczynski
R M
RM
D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D003213:Conditioning, Psychological; D007165:Immunosuppression Therapy; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013997:Time Factors
D008070:Lipopolysaccharides