A nasal whole-cell pertussis vaccine can induce strong systemic and mucosal antibody responses which are not enhanced by cholera toxin.
The immunogenicity of formaldehyde-inactivated Bordetella pertussis (Bp) delivered by the intranasal or colonic-rectal routes in BALB/c mice was studied by immunization four times at weekly intervals with Bp alone, or with Bp mixed with cholera toxin (CT) as a mucosal adjuvant. Mice given Bp subcutaneously, and untreated mice served as controls. Antibody responses in serum, saliva, bronchoalveolar lavage (BAL) fluids and extracts of faeces were measured by enzyme-linked immunosorbent assay. Nasal immunizations with Bp alone induced high levels of IgG antibodies to Bp in serum and BAL fluids, as well as IgA antibodies in serum, saliva, BAL fluids and extracts of faeces. The IgA responses were significantly reduced, and the IgG responses were not increased, when CT was given intranasally together with Bp. However, CT increased the IgA responses to Bp in faeces when both antigens were given rectally, while rectal administration of Bp alone did not induce significant serum or secretory antibody responses. However, when mixed with Bp, the CT itself induced antibodies to CT in serum and samples representing secretions after both nasal and rectal administrations. Thus, Bp is strongly immunogenic when applied intranasally, but not when presented into the intestinal lumen via the rectal route. It appears that CT, which is known to be a mucosal adjuvant and which in itself is a strong mucosal immunogen, will inhibit the immune responses of other strong immunogens when applied on the nasal mucosa.
Berstad
A K
AK
Department of Vaccinology, National Institute of Public Health, Oslo, Norway.
Holst
J
J
Møgster
B
B
Haugen
I L
IL
Haneberg
B
B
D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001886:Bordetella pertussis / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology*; D005260:Female; D018928:Immunity, Mucosal; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010567:Pertussis Vaccine / Q000008:administration & dosage* / Q000276:immunology
D000907:Antibodies, Bacterial; D010567:Pertussis Vaccine; D002772:Cholera Toxin