title: The regulation of Streptococcus mutans glucan-binding protein A expression.
abstract: The S. mutans GBP-A is hypothesized to be constitutively expressed and to contribute to the sucrose-dependent colonization of S. mutans. To investigate GBP-A expression, a reporter gene encoding chloramphenicol acetyltransferase (CAT) was placed downstream of the gbpA promoter and CAT activity was measured under conditions that would be associated with the sucrose-dependent colonization of S. mutans. Expression of GBP-A was optimal under anaerobiosis and neutral pH conditions, and correlated with optimal growth. The addition of sucrose to the growth medium did not elevate the expression of GBP-A.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, Albany Medical College, NY 12208, USA. Jeff_Banas@CCGATEWAY.AMC.EDU
authors.forename: J A
authors.initials: JA
authors.lastname: Banas
authors.forename: H C
authors.initials: HC
authors.lastname: Potvin
authors.forename: R N
authors.initials: RN
authors.lastname: Singh
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D037102:Lectins; D013295:Streptococcus mutans / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D037102:Lectins; C037937:glucan-binding proteins; D013395:Sucrose