Evidence for a role of CRM1 in signal-mediated nuclear protein export.
Chromosome maintenance region 1 (CRM1), a protein that shares sequence similarities with the karyopherin beta family of proteins involved in nuclear import pathway, was shown to form a complex with the leucine-rich nuclear export signal (NES). This interaction was inhibited by leptomycin B, a drug that prevents the function of the CRM1 protein in yeast. To analyze the role of the CRM1-NES interaction in nuclear export, a transport assay based on semipermeabilized cells was developed. In this system, which reconstituted NES-, cytosol-, and energy-dependent nuclear export, leptomycin B specifically blocked export of NES-containing proteins. Thus, the CRM1 protein could act as a NES receptor involved in nuclear protein export.
Ossareh-Nazari
B
B
Institut Curie-CNRS Unité Mixte de Recherche 144, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
Bachelerie
F
F
Dargemont
C
C
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D020672:I-kappa B Proteins; D015151:Immunoblotting; D028884:Karyopherins; D000072000:NF-KappaB Inhibitor alpha; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000737:chemistry / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D000097606:Exportin 1 Protein
D000255:Adenosine Triphosphate; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005231:Fatty Acids, Unsaturated; D020672:I-kappa B Proteins; D028884:Karyopherins; D000072000:NF-KappaB Inhibitor alpha; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D021382:Protein Sorting Signals; D011770:Pyruvate Kinase; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins; D000097606:Exportin 1 Protein; C000606204:NFKBIA protein, human; C038753:leptomycin B