The lysine cluster in the collagen-like domain of the scavenger receptor provides for its ligand binding and ligand specificity.
Scavenger receptors bind modified low-density lipoproteins (LDL) on a collagen-like domain which possesses a lysine cluster at the carboxy end. We previously constructed a receptor model peptide containing the lysine cluster. In the present study, we evaluated the ligand specificity of the receptor model peptide. It selectively bound modified-LDLs, and not LDL. The binding of acetylated-LDL (Ac-LDL) was inhibited by dextran sulfate, fucoidan, and sulfatides in a manner similar to that of the natural receptor. Both polyguanylic and polyinosinic acids inhibit the Ac-LDL binding whereas polycytidylic acid did not. These results indicate that the lysine cluster in the collagen-like domain has important roles in both ligand binding and ligand specificity.
Yamamoto
K
K
New Drug Discovery Research Laboratory, Kanebo Ltd., Osaka, Japan.
Nishimura
N
N
Doi
T
T
Imanishi
T
T
Kodama
T
T
Suzuki
K
K
Tanaka
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D016264:Dextran Sulfate / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008239:Lysine; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D011973:Receptors, LDL / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D013379:Substrate Specificity; D013433:Sulfoglycosphingolipids / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D008024:Ligands; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D010446:Peptide Fragments; D011134:Polysaccharides; D011971:Receptors, Immunologic; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D013433:Sulfoglycosphingolipids; C032629:acetyl-LDL; D003094:Collagen; D016264:Dextran Sulfate; C007789:fucoidan; D008239:Lysine