title: Influence of oxygen in inflation gas during lung ischemia on ischemia-reperfusion injury.
abstract: OBJECTIVE
  Previous studies have reported that hyperinflation during lung ischemia improves pulmonary function after reperfusion. However, it has not been clarified whether hyperinflation itself or oxygen in inflation gas causes good pulmonary function. The aim of this study is to evaluate the effect of oxygen in pulmonary inflation gas during lung ischemia on ischemia-reperfusion injury.
  
  
  METHODS
  Twenty-one mongrel dogs were randomly divided into three groups: the lung during a 90-minute period of warm ischemia was inflated to 30 cm H2O with 100% oxygen in group A and 100% nitrogen in group B; it was not inflated in group C. Pulmonary function and hemodynamics were measured before ischemia and 1, 2, and 3 hours after reperfusion. Total protein and phosphorus of phospholipid in bronchoalveolar lavage fluid were measured 210 minutes after reperfusion. Adenine nucleotide levels in lung tissue were estimated 210 minutes after reperfusion.
  
  
  RESULTS
  No significant differences in pulmonary function and hemodynamics were noted between group A and group B, but these two groups had significantly better pulmonary function and hemodynamics than group C. No significant differences were detected in the concentrations of total protein and phosphorus of phospholipids in bronchoalveolar lavage fluid and in adenine nucleotide levels of lung tissue after reperfusion among the three groups.
  
  
  CONCLUSIONS
  The results indicate that pulmonary inflation during warm ischemia improves pulmonary function and hemodynamics after reperfusion in this model. The effect is caused by inflation itself and is not due to oxygen as a metabolic substrate during warm ischemia.
authors.affiliation: Second Department of Surgery, Sapporo Medical University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Watanabe
authors.forename: N
authors.initials: N
authors.lastname: Kawaharada
authors.forename: K
authors.initials: K
authors.lastname: Kusajima
authors.forename: S
authors.initials: S
authors.lastname: Komatsu
authors.forename: T
authors.initials: T
authors.lastname: Abe
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004285:Dogs; D015633:Extravascular Lung Water / Q000378:metabolism; D006439:Hemodynamics / Q000502:physiology; D008168:Lung / Q000098:blood supply* / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D011652:Pulmonary Circulation / Q000502:physiology; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: D000227:Adenine Nucleotides; D010743:Phospholipids; D010100:Oxygen