title: Recovery of hypothalamic NMDA-induced c-fos expression following neonatal glutamate (MSG) lesions.
abstract: The neonatal brain is susceptible to neurotoxic insult. In a previous report we showed that a single neonatal injection of MSG, known to cause damage in the arcuate nucleus (ARC), induces a precocious yet otherwise normal puberty in female rats. We have examined this ability of the medial basal hypothalamus (MBH) to recover from an excitotoxic insult using the immediate-early gene c-fos as a developmental marker of ARC response to glutamate receptor stimulation with N-methyl-D-aspartate (NMDA). Groups of neonatal (postnatal day (PD) 2) pups were injected with MSG, then stimulated on subsequent days (PD 3-29) with NMDA, known to induce c-fos expression in ARC. Computer-assisted densitometry was used to quantify Fos-like immunoreactivity (FLI) profiles in ARC. Pups treated neonatally with saline (PD 2) showed a robust, age-specific expression of FLI in the ARC following NMDA treatment. The FLI response was absent in the days immediately following an MSG lesion but subsequently recovered up to 75% of maximum by PD 16. Almost full recovery was seen by PD 29. We also examined the ability of the ARC to recover following chronic MSG treatment (PD 2-8), known to induce extensive hypothalamic damage. These pups displayed an unusual response to subsequent NMDA injection, consisting of 5 min cycles of hyper- and hypoactivity. Stimulation with NMDA revealed only a 50% recovery of FLI even at PD 29. In both treatment groups (acute vs. chronic MSG) the zone of recovery (i.e., reappearance of FLI) was initiated close to the third ventricle and with time radiated towards the periphery of the ARC. Some cells which reacquired FLI in the ARC following lesions presented a highly irregular condensed nuclear morphology. We conclude that the recovery of hypothalamic function (i.e., onset of puberty) after a neonatal MSG lesion is coincident with the reappearance of a normal pattern of c-fos expression in response to NMDA stimulation.
authors.affiliation: Department of Obstetrics and Gynaecology, IWK-Grace Health Centre, Halifax, NS, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Natarajan
authors.forename: M
authors.initials: M
authors.lastname: Wilkinson
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D001522:Behavior, Animal / Q000187:drug effects; D003720:Densitometry; D007091:Image Processing, Computer-Assisted; D007267:Injections; D016202:N-Methylaspartate / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009498:Neurotoxins / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012970:Sodium Glutamate / Q000494:pharmacology*
chemical_list: D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D016760:Proto-Oncogene Proteins c-fos; D016202:N-Methylaspartate; D012970:Sodium Glutamate