Essential lymphocyte function associated 1 (LFA-1): intercellular adhesion molecule interactions for T cell-mediated B cell apoptosis by Fas/APO-1/CD95.
B cells are susceptible to Fas ligand (FasL)+ CD4(+) Th1 cell-mediated apoptosis. We demonstrate that blocking the interactions between lymphocyte function associated (LFA)-1 and intercellular adhesion molecule(ICAM)-1 and ICAM-2 completely suppresses Fas-dependent B cell lysis. Antibodies to CD2 and CD48 partially suppress B cell apoptosis, whereas anti-B7.1 and anti-B7.2 antibodies have no effect. Also, B cells from ICAM-1-deficient mice are resistant to FasL+ T cell-mediated death. Our results suggest that LFA-1/ICAM interactions are crucial for Th1 cell-mediated B cell apoptosis and may contribute to the maintenance of B cell homeostasis in vivo.
Wang
J
J
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-1892, USA.
Lenardo
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D017209:Apoptosis; D001402:B-Lymphocytes / Q000166:cytology*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D002859:Chromium Isotopes; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D018417:Th1 Cells / Q000276:immunology / Q000502:physiology*; D019014:fas Receptor / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D002859:Chromium Isotopes; C065289:ICAM2 protein, human; D016169:Lymphocyte Function-Associated Antigen-1; D019014:fas Receptor; D018799:Intercellular Adhesion Molecule-1