Regulation of insulin-like growth factor-binding protein-1 and progesterone secretion from human granulosa-luteal cells: effects of octreotide and insulin.
OBJECTIVE
To examine the effects of the synthetic somatostatin-analogue octreotide and human recombinant insulin on the release of insulin-like growth factor binding protein-1 (IGFBP-1) and P from human granulosa-luteal cells.


METHODS
Primary culture of human granulosa-luteal cells.


METHODS
Academic research laboratory.


METHODS
Women undergoing oocyte retrieval for IVF-ET because of tubal infertility.


METHODS
Octreotide or insulin were added to the cultures; sampling of culture medium was performed after 48 hours.


METHODS
Insulin-like growth factor binding protein-1 and P.


RESULTS
Octreotide significantly inhibited IGFBP-1 (58.8% compared with controls) and P release (66.1% compared with controls). Insulin abolished IGFBP-1 release while stimulating P release (200.7% compared with controls). There was a significant and positive correlation between IGFBP-1 and P levels.


CONCLUSIONS
Octreotide and insulin have a significant effect on human granulosa-luteal cell function in terms of IGFBP-1 and P release. Our results suggest a local ovarian mechanism for the recently observed effects of octreotide in the treatment of women with polycystic ovary syndrome.
Holst
N
N
Department of Obstetrics and Gynecology, Ulleval Hospitals, University of Oslo, Norway.
Kierulf
K H
KH
Seppälä
M
M
Koistinen
R
R
Jacobsen
M B
MB
D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000378:metabolism*; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism; D015282:Octreotide / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism*
D007328:Insulin; D018970:Insulin-Like Growth Factor Binding Protein 1; D011374:Progesterone; D015282:Octreotide