title: Study of correlations between fine structure of bonded layer and affinity properties of silicas with attached cyclic oligopeptides.
abstract: The cyclic oligopeptides bacitracin A and gramicidin-S were immobilized on epoxy-, tosyloxy- and halogenoaklyl-activated silicas. The sorbents obtained were tested for affinity binding of proteinases. The effects of spacer length, ligand loading and the character of ligand attachment (one- or poly-site) on the biospecific characteristics have been studied.
authors.affiliation: Chemistry Department, Moscow State University, Vorob yovy Gory, Russia.
authors.forename: P G
authors.initials: PG
authors.lastname: Mingalyov
authors.forename: N V
authors.initials: NV
authors.lastname: Orishchenko
authors.lastname: Fadeev AYu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D001414:Bacitracin / Q000737:chemistry* / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D004578:Electron Spin Resonance Spectroscopy; D006096:Gramicidin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008024:Ligands; D009842:Oligopeptides / Q000737:chemistry*; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry*; D013381:Subtilisins / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D008024:Ligands; D009842:Oligopeptides; D001414:Bacitracin; D006096:Gramicidin; D012822:Silicon Dioxide; C026461:bacitracin A; D013381:Subtilisins; D014357:Trypsin; D013917:Thrombin