Appearance of lipidic signals in "stressed" cells: study of myogenic cultured cells by two-dimensional nuclear magnetic resonance.
The proton NMR spectra of regenerating muscle shoe high resolution fatty acid signals as the spectra of other stressed cells such as ischemic cardiac cells, stimulated immune cells or malignant cells. We report here the in vitro study, by 2D 1H NMR, of the fusion of myogenic cells. High resolution fatty acid signals are only detected during cell fusion, demonstrating a higher mobility of the acyl chains.
Bleneau
S
S
Laboratoire de RMN Biologique, ICSN, CNRS, Gif sur Yvette, France.
Gillet
B
B
Verre-Sebrie
C
C
Beloeil
J C
JC
D000818:Animals; D002459:Cell Fusion; D002478:Cells, Cultured; D019344:Lactic Acid / Q000737:chemistry; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008809:Mice, Inbred C3H; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D012038:Regeneration; D013654:Taurine / Q000737:chemistry
D008055:Lipids; D013654:Taurine; D019344:Lactic Acid