Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjögren's syndrome.
OBJECTIVE
To identify a Golgi complex autoantigen bound by Sjögren's syndrome (SS) autoantibodies.


METHODS
Serum from a patient with secondary SS and anti-Golgi antibodies was used as a probe to isolate a complementary DNA (cDNA) insert from a HeLa cDNA library.


RESULTS
A 3.7-kb cDNA encoding a 56-kd recombinant protein was immunoprecipitated by the human anti-Golgi serum and immune rabbit serum. Western blot analysis showed that the immune rabbit sera recognized a protein of 97 kd (golgin-97), suggesting that the isolated clone contained a partial cDNA. The 5' upstream sequence was obtained by rapid amplification of the cDNA ends. The complete cDNA contained 4,860 basepairs, encoding a protein with a calculated Mr of 88 kd. Antibodies to golgin-97 were found in 12 (20%) of 60 sera known to have anti-Golgi autoantibodies, and the majority of these sera (8 of 12, or 75%) were from patients who had secondary SS.


CONCLUSIONS
Golgin-97 is a unique Golgi complex antigen that appears to be a target of SS autoantibodies.
Griffith
K J
KJ
University of Calgary, Alberta, Canada.
Chan
E K
EK
Lung
C C
CC
Hamel
J C
JC
Guo
X
X
Miyachi
K
K
Fritzler
M J
MJ
D000328:Adult; D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000032:analysis; D001324:Autoantigens / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000302:isolation & purification; D005260:Female; D006056:Golgi Apparatus / Q000276:immunology*; D000077614:Golgi Matrix Proteins; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012859:Sjogren's Syndrome / Q000276:immunology*
D001323:Autoantibodies; D001324:Autoantigens; D017931:DNA Primers; D018076:DNA, Complementary; D000077614:Golgi Matrix Proteins; C450721:Golgi complex autoantigen, 97-kDa