Effect of intraocular lens design on migration of lens epithelial cells onto the posterior capsule.
OBJECTIVE
To find the conditions that prevent posterior capsule opacification through in vitro analysis of the relationship between intraocular lens (IOL) optic configuration and lens epithelial cell (LEC) migration.


METHODS
Division of Morphogenesis, Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.


METHODS
In a preliminary experiment, we measured the size of the capsular bag of rabbits at 8, 16, 20, and 26 weeks of age. The size of a 20-week-old capsular bag was the same size as the capsular bag in senescent Japanese eyes. We isolated the capsular bags in 20- and 8-week-old rabbits. The bags along with a biconvex (BC), convex-plano (CP), or no IOL (C) were cultured, and the eyes were divided into one of six groups (8W-C, 8W-BC, 8W-CP, 20W-C, 20W-BC and 20W-CP), each including six specimens.


RESULTS
Two specimens in the 8W-CP group completely blocked LEC migration at the optic edge. All specimens in the 20W-CP group and one in the 20W-BC group showed cell aggregation along the optic edge. None of the other specimens in the BC and C groups blocked migration or showed cell aggregation.


CONCLUSIONS
In the rabbit-model study, the convex-plano lens was superior to the biconvex lens in inhibiting migration of LECs. A firm contact between the IOL and the posterior capsule blocked the migration.
Nagamoto
T
T
Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.
Eguchi
G
G
D000818:Animals; D002386:Cataract / Q000473:pathology / Q000517:prevention & control; D002449:Cell Aggregation; D002465:Cell Movement; D002478:Cells, Cultured; D004848:Epithelium / Q000473:pathology; D007903:Lens Capsule, Crystalline / Q000473:pathology*; D007908:Lens, Crystalline / Q000473:pathology*; D007910:Lenses, Intraocular; D008858:Microscopy, Phase-Contrast; D011474:Prosthesis Design; D011817:Rabbits