Expression of TGF-beta superfamily receptors in dental pulp.
Transforming growth factor-beta (TGF-beta) superfamily members and their cell-surface receptors may play inductive and/or regulatory roles in tooth development and repair. It will be important to identify the complete set of TGF-beta superfamily receptors, to examine their temporal and spatial localization during tooth development, and to elucidate the cascade of molecular events of tooth formation induced by the TGF-beta superfamily. In this report, we have cloned the cDNAs encoding potential receptors for TGF-beta superfamily members in rat incisor pulp and bovine adult pulp which are regarded as embryonic and adult pulp, respectively. We analyzed poly (A)+ RNA from rat incisor pulp and bovine adult pulp by reverse-transcriptase/polymerase chain-reaction (RT-PCR), using a degenerate primers corresponding to the most conserved amino acid sequences in the intracellular serine/threonine kinase of type I or type II like kinase-1 (ALK-1), ALK-2, ALK-3 (bone morphogenetic protein receptor type IA, BMPR-IA), ALK-4 (B1), ALK-5, ALK-6 (BMPR-IB), and BMPR-II (BMP type II receptor) was found to be in dental pulp. Northern blot analysis further detected TGF-beta type II receptor (T beta R-II) mRNA transcript in addition to the above-identified receptors. These results provide the first evidence of multiple type I and type II receptors for TGF-beta s, activins, and BMPs expressed in embryonic and adult pulp, implicating diverse function in tooth development and pulp tissue repair.
Toyono
T
T
Laboratory of Molecular Gene Technics, Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Nakashima
M
M
Kuhara
S
S
Akamine
A
A
D029404:Activin Receptors; D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D003782:Dental Pulp / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances / Q000235:genetics; D007180:Incisor; D007265:Inhibins / Q000235:genetics; D008969:Molecular Sequence Data; D009805:Odontogenesis / Q000235:genetics; D011061:Poly A / Q000235:genetics; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017978:Receptors, Growth Factor / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis / Q000235:genetics*; D013997:Time Factors; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000235:genetics*; D014945:Wound Healing / Q000235:genetics
D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D006133:Growth Substances; D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D028341:Activins; D011061:Poly A; D007265:Inhibins; D012313:RNA; D017346:Protein Serine-Threonine Kinases; D029404:Activin Receptors