Proadrenomedullin N-terminal 20 peptide (PAMP) inhibits proliferation of human neuroblastoma TGW cells.
We investigated the effects of proadrenomedullin N-terminal 20 peptide (PAMP) and adrenomedullin (AM) on the growth of human neuroblastoma TGW cells. Both PAMP and AM inhibited growth and DNA synthesis in neuroblastoma cells. Calcitonin gene-related peptide (CGRP)(8-37), an antagonist to CGRP, abolished the inhibitory effect of AM on growth and DNA synthesis of neuroblastoma cells but did not affect that of PAMP. AM(22-52), an antagonist to AM, also reversed the effect of AM. On the other hand, pertussis toxin (PTX) and omega-conotoxin GIVA blocked the effect of PAMP alone. Thus, PAMP inhibits the growth of neuroblastoma cells by inhibiting N-type Ca2+ channels through PTX-sensitive G protein-coupled receptors, which is different mechanism of AM-induced inhibition of the cell growth.
Ando
K
K
Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan. katsua-tky@umin.u-tokyo.ac.jp
Omi
N
N
Shimosawa
T
T
Fujita
T
T
D053607:Adrenomedullin; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D009447:Neuroblastoma; D010446:Peptide Fragments / Q000494:pharmacology / Q000633:toxicity*; D010455:Peptides / Q000633:toxicity*; D037342:Pertussis Toxin; D011506:Proteins / Q000633:toxicity*; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D004273:DNA, Neoplasm; D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; D010566:Virulence Factors, Bordetella; C058636:calcitonin gene-related peptide (8-37); D053607:Adrenomedullin; D037342:Pertussis Toxin; D015740:Calcitonin Gene-Related Peptide