Attachment of piliated, Opa- and Opc- gonococci and meningococci to epithelial cells elicits cortical actin rearrangements and clustering of tyrosine-phosphorylated proteins.
Attachment of piliated Neisseria gonorrhoeae or Neisseria meningitidis cells to A431, Chang, HEC-1-B, or polarized T(84) cells triggers rearrangements of cortical microfilaments and the accumulation of phosphotyrosine-containing proteins at sites of bacterial contact. Actin stress fibers and the microtubule network remain unaltered in infected cells. The rearrangements reported here are triggered by piliated, Opa- and Opc- strains and also by nonpiliated gonococci (GC) that produce the invasion-associated OpaA protein. Thus, neisserial adhesion via either of at least two different adhesins can trigger cortical rearrangements. In contrast, these rearrangements are not triggered by nonadherent GC or meningococcal strains, by heat-killed or chloramphenicol-treated GC strains, or by Escherichia coli recombinants that adhere to cells via GC OpaA or Opal fusion proteins, suggesting that additional neisserial components are involved. Immunoblotting experiments did not detect consistent increases in the phosphorylation of specific proteins. Possible biological implications of these Neisseria-induced cortical rearrangements are discussed.
Merz
A J
AJ
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201-3098, USA. merza@ohsu.edu
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D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D000199:Actins / Q000378:metabolism / Q000648:ultrastructure; D000942:Antigens, Bacterial / Q000235:genetics; D001422:Bacterial Adhesion / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology / Q000648:ultrastructure; D010861:Fimbriae, Bacterial; D008870:Microtubules / Q000648:ultrastructure; D009343:Neisseria / Q000235:genetics / Q000472:pathogenicity*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000472:pathogenicity; D009345:Neisseria meningitidis / Q000235:genetics / Q000472:pathogenicity; D010750:Phosphoproteins / Q000378:metabolism; D019000:Phosphotyrosine / Q000378:metabolism
D000199:Actins; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D010750:Phosphoproteins; C050308:opacity proteins; C074517:opc protein, bacteria; D019000:Phosphotyrosine