Identification and NH2-terminal amino acid sequences of DnaK and groEL homologues in moderate eubacterial halophiles.
We have identified 2 DnaK and 3 GroEL homologues from moderately halophilic Acinetobacter, Pseudomonas, and Planococcus species by partial purification using an ATP-agarose column and by the analysis and similarity search of these NH2-terminal amino acid sequences. Although these bacteria required 1 to 2M NaCl for growth, these DnaK and GroEL homologues did not require high salt to bind to the ATP column, thus suggesting that these chaperones did not require high salts for their biochemically activities.
Tokunaga
M
M
Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, Japan.
Matsuoka
K
K
Tokunaga
H
H
D000150:Acinetobacter / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D018834:Chaperonin 60 / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis*; D015151:Immunoblotting; D008835:Micrococcaceae / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*
D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; C063842:dnaK protein, E coli