Isolation and expression of the gene which encodes a novel enzyme with polymethoxygalacturonate-degrading activity in Trichosporon penicillatum.
The novel gene named PSX1, encoding a new protopectinase with the polymethoxygalacturonase activity, was isolated from Trichosporon penicillatum. Nucleotide sequencing revealed that the PSX1 gene is composed of 1080 bases (360 amino acids, 38,747 Da). The N-terminal amino acid sequences of the open reading frame correspond to a signal peptide and propeptide processed by a Kex2-like proteinase. Mature PPase SX1 was composed of 334 amino acids (36,121 Da). PPase SX1 produced by a S. cerevisiae transformant harboring the PSX1 gene degraded methoxylated polygalacturonic acid as a substrate, but not degraded unmethoxylated polygalacturonic acid.
Sakai
T
T
Department of Applied Biochemistry, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
Sirasaka
N
N
Hirano
H
H
Kishida
M
M
Kawasaki
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000737:chemistry; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011133:Polysaccharide-Lyases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D021382:Protein Sorting Signals / Q000096:biosynthesis / Q000737:chemistry; D012441:Saccharomyces cerevisiae; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014250:Trichosporon / Q000201:enzymology* / Q000235:genetics*
D004792:Enzyme Precursors; D021382:Protein Sorting Signals; D006026:Glycoside Hydrolases; C083413:protopectinase; D011133:Polysaccharide-Lyases; C027349:pectin lyase