Eicosapentaenoic acid and epidermal growth factor modulation of human breast cancer cell adhesion.
Cancer cell adhesion to the subendothelial extracellular matrix (ECM) is an important step in metastasis formation. The effect of epidermal growth factor (EGF) and eicosapentaenoic acid (EPA) on adhesion of the highly metastatic MDA-MB-231 human breast cancer cell line to ECM components was examined in the present study. MDA-MB-231 cells exhibited a dose-dependent decrease in adhesion to Matrigel when treated with EGF. EGF and EPA, alone or in combination, decreased adhesion to Matrigel, fibronectin and type IV collagen. These results suggest that decreased adhesion to ECM substrates by combined EPA and EGF treatment may be the result of a common post-receptor signaling pathway.
German
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Department of Nutrition Sciences, University of Alabama at Birmingham, 35294-3360, USA.
Johanning
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D001943:Breast Neoplasms; D002448:Cell Adhesion / Q000187:drug effects*; D003094:Collagen; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004347:Drug Interactions; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D005260:Female; D005353:Fibronectins; D006801:Humans; D007797:Laminin; D009362:Neoplasm Metastasis; D011509:Proteoglycans; D014407:Tumor Cells, Cultured
D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D004815:Epidermal Growth Factor; D003094:Collagen; D015118:Eicosapentaenoic Acid