Detrimental effect of hydrosalpinx fluid on the development and blastulation of mouse embryos in vitro.
OBJECTIVE
To study the effects of human hydrosalpinx fluid on mouse embryo blastulation rate.


METHODS
Comparison of mouse embryo blastulation rate in media containing increasing concentrations of hydrosalpinx fluid.


METHODS
Tertiary care center.


METHODS
Women undergoing laparoscopic evaluation or treatment for infertility noted to have hydrosalpinx or paratubal cyst.


METHODS
Exposure of mouse embryos to hydrosalpinx or paratubal cyst fluid collected during laparoscopy.


METHODS
Blastulation rate of mouse embryos.


RESULTS
Culture of mouse embryos at 0% (controls), 0.3%, 0.6%, and 0.9% hydrosalpinx fluid concentrations demonstrated significantly lower blastulation rate at each level compared with the controls.


CONCLUSIONS
Hydrosalpinx fluid is highly embryotoxic.
Sachdev
R
R
Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey (UMDNJ)-Robert Wood Johnson Medical School, New Brunswick 08901, USA.
Kemmann
E
E
Bohrer
M K
MK
el-Danasouri
I
I
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D005184:Fallopian Tube Diseases / Q000503:physiopathology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D011247:Pregnancy