cDNA cloning of a short type of multidrug resistance protein homologue, SMRP, from a human lung cancer cell line.
Members of the ATP binding cassette (ABC) superfamily are involved in the energy-dependent transport of a wide variety of substrates including anticancer agents across the membranes. We have cloned a cDNA fragment including a novel ABC sequence from a cisplatin-resistant human lung adenocarcinoma cell line, PC-14/CDDP, by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers and screened a cDNA library using the cDNA fragment as a probe. A full-length cDNA clone, BM4.8, was obtained. Sequence analysis showed that the cDNA encoded a short type of multidrug resistance protein homologue, SMRP, by computed homology search. SMRP was composed of 946 amino acids and had two ABCs with walker A and B motifs. This gene was mapped on chromosome 3 at band q27 by fluorescence in situ hybridization (FISH) analysis and was found to be expressed in various tissues by Northern blot analysis.
Suzuki
T
T
Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
Nishio
K
K
Sasaki
H
H
Kurokawa
H
H
Saito-Ohara
F
F
Ikeuchi
T
T
Tanabe
S
S
Terada
M
M
Saijo
N
N
D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D002945:Cisplatin / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D018432:Drug Resistance, Multiple; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D018528:ATP-Binding Cassette Transporters; D002945:Cisplatin; D018076:DNA, Complementary; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009363:Neoplasm Proteins