A useful method for the synthesis of 8 azido-guanosine tri- and tetraphosphate: important substrates for many enzymatic reactions.
8-N3 GMP was synthesised from 8-BrGMP by the addition of LiN3. 8-N3GMP was then phosphorylated to N3-GDP and N3-GTP by controlled enzymatic reaction. 8-N3GDP can be converted to N3-ppGpp with crude Rel A, which phosphorylates the 3'-OH of GDP.
Nagesh
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Centre for Cellular and Molecular Biology, Hyderabad, India.
Chatterji
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D002855:Chromatography, Thin Layer; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000302:isolation & purification
D006160:Guanosine Triphosphate