Insertional inactivation of a Tet(K)/Tet(L) like transporter does not eliminate tetracycline resistance in Bacillus cereus.
Bacillus cereus ATCC 10987 and ATCC 14579 can be induced to high levels of resistance to tetracycline. The chromosomal B. cereus gene bctl encodes a transmembrane protein with homology to Gram-positive tetracycline efflux proteins and relation to other members of the major facilitator superfamily of transport proteins. A mutant strain containing an insertionally inactivated bctl gene did not show impaired tetracycline resistance. No additional altered phenotype was observed in the mutant. Accumulation studies suggested that the resistance mechanism involves a reduced sensitivity to intracellular tetracycline.
Okstad
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Biotechnology Centre of Oslo, University of Oslo, Blindern, Norway.
Grønstad
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Lindbäck
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Kolstø
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D001409:Bacillus cereus / Q000187:drug effects* / Q000235:genetics; D001426:Bacterial Proteins / Q000502:physiology*; D001483:Base Sequence; D002352:Carrier Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D013752:Tetracycline / Q000493:pharmacokinetics*; D013753:Tetracycline Resistance
D001426:Bacterial Proteins; D002352:Carrier Proteins; D013752:Tetracycline