title: Translational repression by a transcriptional elongation factor.
abstract: One of the classical positive regulators of gene expression is bacteriophage lambda N protein. N regulates the transcription of early phage genes by participating in the formation of a highly processive, terminator-resistant transcription complex and thereby stimulates the expression of genes lying downstream of transcriptional terminators. Also included in this antiterminating transcription complex are an RNA site (NUT) and host proteins (Nus). Here we demonstrate that N has an additional, hitherto unknown regulatory role, as a repressor of the translation of its own gene. N-dependent repression does not occur when NUT is deleted, demonstrating that N-mediated antitermination and translational repression both require the same cis-acting site in the RNA. In addition, we have identified one nut and several host mutations that eliminate antitermination and not translational repression, suggesting the independence of these two N-mediated mechanisms. Finally, the position of nutL with respect to the gene whose expression is repressed is important.
authors.affiliation: ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
authors.forename: H R
authors.initials: HR
authors.lastname: Wilson
authors.forename: L
authors.initials: L
authors.lastname: Kameyama
authors.forename: J G
authors.initials: JG
authors.lastname: Zhou
authors.forename: G
authors.initials: G
authors.lastname: Guarneros
authors.forename: D L
authors.initials: DL
authors.lastname: Court
mesh_terms: D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D005814:Genes, Viral; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis / Q000187:drug effects*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; C059417:N protein, Bacteriophage lambda; D012367:RNA, Viral; D014157:Transcription Factors; D054334:Viral Regulatory and Accessory Proteins