Identification of a naturally occurring peroxidase-lipoxygenase fusion protein.
A distant relative of catalase that is specialized for metabolism of a fatty acid hydroperoxide was identified. This heme peroxidase occurs in coral as part of a fusion protein, the other component of which is a lipoxygenase that forms the hydroperoxide substrate. The end product is an unstable epoxide (an allene oxide) that is a potential precursor of prostaglandin-like molecules. These results extend the known chemistry of catalase-like proteins and reveal a distinct type of enzymatic construct involved in the metabolism of polyunsaturated fatty acids.
Koljak
R
R
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232-6602, USA.
Boutaud
O
O
Shieh
B H
BH
Samel
N
N
Brash
A R
AR
D000595:Amino Acid Sequence; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001665:Binding Sites; D002374:Catalase / Q000737:chemistry; D002384:Catalysis; D003001:Cloning, Molecular; D003063:Cnidaria / Q000201:enzymology* / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000737:chemistry; D008084:Lipoxygenase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010544:Peroxidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D011994:Recombinant Proteins; D016718:Arachidonic Acid; D006861:Hydrogen Peroxide; D010544:Peroxidases; D002374:Catalase; D009195:Peroxidase; D008084:Lipoxygenase; C108279:Allene oxide synthase-lipoxygenase protein, Plexaura homomalla; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase