title: Tandem arrangement of two genes encoding kinetoplast-associated H1 histone-like proteins.
abstract: Crithidia fasciculata proteins p18 and p17 are associated with kinetoplast DNA and are encoded by genes KAP2 and KAP3, respectively. Polymerase chain reaction (PCR) amplification using primers within the coding sequences of each gene revealed that the KAP2 and KAP3 genes are linked on the same chromosomal DNA and are separated by a 1.8 kb intergenic region containing several long homopolymer tracts. The KAP2 gene has a 3'UTR of more than 1.1 kb or almost three times as long as the KAP2 coding sequence. Several restriction enzyme polymorphisms in this region of the chromosome are the result of sequence differences between the two alleles of the KAP2 gene. The predicted amino-acid sequences of alleles KAP2-1 and KAP2-2 differ by three non-conservative amino acid substitutions in the highly basic carboxyl tail of the protein and suggest that the protein products could have different physical and biological properties. The KAP2 and KAP3 genes have different patterns of mRNA expression during the cell cycle with the KAP3 transcript varying periodically during the cell cycle in the same manner as transcripts of several kinetoplast and nuclear DNA replication genes.
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles 90095-1570, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Hines
authors.forename: D S
authors.initials: DS
authors.lastname: Ray
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002453:Cell Cycle / Q000235:genetics; D003001:Cloning, Molecular; D016831:Crithidia fasciculata / Q000235:genetics*; D018105:DNA, Kinetoplast / Q000378:metabolism*; D016054:DNA, Protozoan / Q000302:isolation & purification; D017125:Genes, Protozoan; D008040:Genetic Linkage; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D006657:Histones; C097692:KAP2 protein, Crithidia fasciculata; C097693:KAP3 protein, Crithidia fasciculata; D015800:Protozoan Proteins; D012333:RNA, Messenger