A two-dimensional thin-layer chromatography procedure for simultaneous separation of ceramide and diacylglycerol species.
We have developed a novel, simple, and rapid, two-dimensional thin-layer chromatography method to separate 1,2-, 1,3-diacylglycerols and ceramides containing alpha-hydroxy and normal fatty acids from other neutral lipids on one 10 x 10 cm precoated silica gel plate. The three solvent systems used in succession leave the phospholipids at the origin and separate neutral lipids of interest into component species. We have applied this method to incorporation of 9,10-[3H]myristic acid into lipids of gills from sea bass and obtained results that are similar and comparable to those obtained by described methods.
Bodennec
J
J
Institut Michel Pacha, Universite Claude Bernard, Lyon I, La Seyne sur Mer, France.
Brichon
G
G
Koul
O
O
El Babili
M
M
Zwingelstein
G
G
D000818:Animals; D001492:Bass / Q000378:metabolism; D002518:Ceramides / Q000737:chemistry / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004075:Diglycerides / Q000737:chemistry / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005880:Gills / Q000737:chemistry / Q000378:metabolism; D019814:Myristic Acid / Q000378:metabolism; D014316:Tritium
D002518:Ceramides; D004075:Diglycerides; D019814:Myristic Acid; D014316:Tritium