[Evaluation of enzyme-linked immunosorbent assay (ELISA) in milk powder contaminated with known concentrations of aflatoxin M1].
The evaluation of commercially available test systems of competitive enzyme-linked immunosorbent assay (ELISA), for Aflatoxin M1 (AFM1) was performed in experimental conditions, through repeated analysis, in samples of milk powder contaminated with known concentrations of the toxin. Recoveries of AFM1 added to milk at levels of 0.10, 0.20, 0.50 and 1.00 ng/ml were 83.0%, 87.5%, 103.0% and 111.8% respectively. Relative standard deviations for the above mentioned concentrations were 65.5%, 31.8%, 10.9% and 13.6%, respectively (n = 10, per spiking level). According to these results the ELISA is appropriate for AFM1 research and/or surveying, mainly for concentrations between 0.20-1.00 ng/ml.
de Oliveira
C A
CA
Departamento de Produção Animal da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Brasil.
Germano
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PM
D016607:Aflatoxin M1 / Q000302:isolation & purification*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D016014:Linear Models; D008892:Milk / Q000737:chemistry*
D016607:Aflatoxin M1