Expression of angiotensin converting enzyme and chymase in human atria.
BACKGROUND
A cardiac angiotensin II-generating system has been suspected to be involved in various cardiac pathological conditions. Both angiotensin converting enzyme and human chymase can convert angiotensin I to angiotensin II.


OBJECTIVE
To clarify the relative contributions of these two enzymatic pathways to angiotensin II generation in vivo.


METHODS
We assessed the expression levels of messenger RNA (mRNA) for collagen type I alpha, transforming growth factor-beta(1), brain natriuretic peptide, angiotensin converting enzyme and chymase in right atrial appendages by competitive polymerase chain reaction and Northern blot analyses. Correlations among the concentrations of these mRNA were analysed to obtain insight that might be important in understanding the formation of angiotensin II in atrial tissue.


RESULTS
The collagen type I alpha and brain natriuretic peptide mRNA concentrations were correlated significantly to the mean pulmonary arterial pressure. Multivariate regression analysis revealed that the collagen type I alpha mRNA concentration could be explained in terms of the brain natriuretic peptide (P = 0.0005) and angiotensin converting enzyme (P = 0.0084) mRNA concentrations (r = 0.598, P < 0.0001). The chymase mRNA concentration had no significant correlation to the collagen type I alpha mRNA concentration. Moreover, multiple regression analysis revealed that the transforming growth factor-beta(1) mRNA concentration could be explained in terms of the angiotensin converting enzyme mRNA concentration alone (r = 0.424, P = 0.014).


CONCLUSIONS
The present results suggest that the level of angiotensin converting enzyme affects the tissue angiotensin II level in human atria; however, we could obtain no evidence that chymase is important in determining the tissue angiotensin II level.
Ohmichi
N
N
First Department of Internal Medicine, Shiga University of Medical Sciences, Japan.
Iwai
N
N
Kinoshita
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D001794:Blood Pressure; D015152:Blotting, Northern; D002648:Child; D002675:Child, Preschool; D053818:Chymases; D003094:Collagen / Q000378:metabolism; D005260:Female; D006325:Heart Atria / Q000201:enzymology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012697:Serine Endopeptidases / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D020097:Natriuretic Peptide, Brain; D003094:Collagen; D007703:Peptidyl-Dipeptidase A; D012697:Serine Endopeptidases; D053818:Chymases