Induction of anti-GM1 ganglioside antibodies by Campylobacter jejuni lipopolysaccharides.
A frequent association exists between acute motor neuropathy, antecedent Campylobacter jejuni (CJ) and anti-GM ganglioside antibodies. Despite the chemical and immunological similarity between CJ lipopolysaccharides (LPS) and GM1, the mechanism of induction of anti-GM1 antibodies is still unclear. We used CJ LPS to immunize rats, mice and immunodeficient mice lacking in NK, CD8+ or T-cell populations. None of these animals developed significant anti-GM1 titers. However, rats immunized with keyhole limpet hemocyanin which contains the cross-reactive sugar epitope Gal(beta1-3)GalNAc developed high titers of IgM anti-GM1 antibodies. This occurred only after these rats were given an intraperitoneal injection of CJ LPS. These results suggest that a glycoprotein antigenic stimulus can induce B-cells which are autoreactive to ganglioside but which remain anergic. A second stimulus with a cross-reactive LPS can then overcome the anergy to induce autoantibody production. A similar mechanism may explain the occurrence of GM1 antibodies in patients after CJ enteritis.
Wirguin
I
I
Department of Neurology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA. wirguin@bgumail.bgu.ac.il
Briani
C
C
Suturkova-Milosevic
L
L
Fisher
T
T
Della-Latta
P
P
Chalif
P
P
Latov
N
N
D000276:Adjuvants, Immunologic; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D000917:Antibody Formation; D016123:Campylobacter jejuni / Q000378:metabolism*; D003429:Cross Reactions; D005260:Female; D005677:G(M1) Ganglioside / Q000276:immunology*; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology; D007114:Immunization; D007075:Immunoglobulin M / Q000032:analysis; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D051381:Rats; D011917:Rats, Inbred Lew
D000276:Adjuvants, Immunologic; D000906:Antibodies; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D005677:G(M1) Ganglioside; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin