Nucleotide sequence of cDNA fragments coding for the FAD-,glycerophosphate- and calcium-binding domains of human islet mitochondrial glycerophosphate dehydrogenase.
The cDNA fragments coding for the FAD-, glycerophosphate- and calcium-binding domains of mitochondrial glycerophosphate dehydrogenase (mGDH) were synthetized using RNA extracted from freshly isolated pancreatic islets of a normal subject and two-non-insulin-dependent diabetic patients. Single strand conformation polymorphism analysis of the PCR products yielded the same mobility as control cDNA probes. Likewise, the nucleotide sequence and corresponding amino acid sequence were identical to the normal gene bank sequence. These findings argue against the presence, in pancreatic islets, of an mGDH isoform distinct from that previously characterized in extrapancreatic organs.
Novials
A
A
Diabetes Unit, Hospital Clinic, Barcelona University, Spain.
Franco
C
C
Malaisse
W J
WJ
Gomis
R
R
D000368:Aged; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D018076:DNA, Complementary; D003920:Diabetes Mellitus / Q000201:enzymology; D005260:Female; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism*; D006801:Humans; D007515:Islets of Langerhans / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D005994:Glycerophosphates; D005182:Flavin-Adenine Dinucleotide; D005993:Glycerolphosphate Dehydrogenase; D002118:Calcium