title: HIV-antibody detection in vitreous humor and serum by enzyme immunosorbent assay and particle agglutination test.
abstract: OBJECTIVE
  To compare enzyme immunosorbent assay (EIA) and the particle agglutination (PA) test for the detection of antibodies to the human immunodeficiency virus (HIV) in cadaveric vitreous humor and serum.
  
  
  METHODS
  Seventeen cadavers presenting pathologic signs of acquired immunodeficiency syndrome (AIDS) at autopsy and 33 without AIDS signs were evaluated. Consistency (k) and significance (p) between the diagnostic tests were established.
  
  
  RESULTS
  The right and left eyes of each of 48 bilaterally tested cadavers presented identical diagnostic results in all laboratory tests. All tests were positive for antibodies in the AIDS cadavers except for two EIA-negative vitreous results. Three autopsy-negative cadavers showed by both EIA and PA testing positive results in serum and negative in vitreous. Two of them showed a serum-positive Western blot.
  
  
  CONCLUSIONS
  Serum EIA anti-HIV antibody detection test is the current gold standard for evaluating corneal donor material. Neither the PA test nor EIA should be used for vitreous testing because of their low sensitivity.
authors.affiliation: Department of Ophthalmology, Federal University of São Paulo (UNIFESP/EPM), Brasil.
authors.forename: P
authors.initials: P
authors.lastname: Schor
authors.forename: E H
authors.initials: EH
authors.lastname: Sato
authors.forename: A
authors.initials: A
authors.lastname: Kuroda
authors.forename: P S
authors.initials: PS
authors.lastname: Barros
authors.forename: L A
authors.initials: LA
authors.lastname: Marcopito
authors.forename: R
authors.initials: R
authors.lastname: Belfort
mesh_terms: D000328:Adult; D000372:Agglutination Tests / Q000379:methods*; D002102:Cadaver; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015483:HIV Antibodies / Q000032:analysis*; D015658:HIV Infections / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D014822:Vitreous Body / Q000276:immunology* / Q000821:virology
chemical_list: D015483:HIV Antibodies