title: Chemical composition and microstructure of uroliths and urinary sediment crystals associated with the feeding of high-level cottonseed meal diet to water buffalo calves.
abstract: The chemical composition and microstructure of seven uroliths and four urinary sediment samples associated with the feeding of high-level cottonseed meal diet to buffalo calves were examined by chemical qualitative analyses, scanning electron microscopy (SEM), X-ray diffraction, and X-ray energy dispersive spectrometry (EDS). Struvite was a major component of kidney stones and of some bladder stones. The kidney stone sample appeared cracked under low power under SEM, aggregated into tiny balls under high power, and as a blade-like structure under even higher power. The bladder stone samples appeared finely granular or granular with various forms of prismatic crystals. The urinary sediments were prismatic crystals, with granules. The newly found prismatic crystals, which were rich in potassium and similar to struvite in crystal structure, were identified as potassium magnesium phosphate (KMgPO4.6H2O) in some bladder stones and urinary sediments. However, crystals which contained Mg and P only, which had been used for struvite identification, were not found by EDS examination in urinary sediments from fresh urine samples of buffalo calves fed the high-level cottonseed meal diet.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, People Republic China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.forename: X
authors.initials: X
authors.lastname: Shen
authors.forename: C
authors.initials: C
authors.lastname: Lin
authors.forename: G
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002020:Buffaloes; D003369:Cottonseed Oil; D003460:Crystallization; D004032:Diet / Q000662:veterinary*; D008274:Magnesium / Q000032:analysis / Q000737:chemistry; D017616:Magnesium Compounds / Q000032:analysis / Q000737:chemistry; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods / Q000662:veterinary; D010710:Phosphates / Q000032:analysis / Q000737:chemistry; D010758:Phosphorus / Q000032:analysis / Q000737:chemistry; D011188:Potassium / Q000032:analysis / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission / Q000379:methods / Q000662:veterinary; D000069877:Struvite; D014545:Urinary Calculi / Q000737:chemistry / Q000648:ultrastructure* / Q000662:veterinary*; D014556:Urine / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D003369:Cottonseed Oil; D017616:Magnesium Compounds; D010710:Phosphates; D010758:Phosphorus; D000069877:Struvite; D008274:Magnesium; D011188:Potassium