[Transfer of the functional genes into eucaryotic cells by neutral phospholipid liposomes].
Transfection of plasmid DNAs containing b-galactosidase gene (pQE-LacZ) or alkaline phosphatase (pCSEAP) into L929 cell line using was studied. The complexes between plasmid DNA and liposomes containing Ca ions and glycyrrhizic acid or &-tocopherol caused successful transfection of functional genes into L929 cells. The efficiency of transfection of plasmid DNAs into L929 cells using polynucleotide-metallo(II)-liposome complexes were 30-50% from the efficiency value of calcium phosphate coprecipitation transfection.
Zhdanov
R I
RI
Buneeva
O A
OA
Podobed
O V
OV
Kutsenko
N G
NG
Tsvetkova
T A
TA
Lavrenova
T P
TP
D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D002118:Calcium; D017930:Genes, Reporter; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives; D019695:Glycyrrhizic Acid; D007739:L Cells; D008081:Liposomes; D051379:Mice; D010957:Plasmids; D013386:Succinates; D014162:Transfection / Q000379:methods*; D014810:Vitamin E; D001616:beta-Galactosidase / Q000235:genetics
D008081:Liposomes; D013386:Succinates; D014810:Vitamin E; D019695:Glycyrrhizic Acid; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase; D006034:Glycyrrhetinic Acid; D002118:Calcium