Characterization and expression of two chicken cDNAs encoding ubiquitin fused to ribosomal proteins of 52 and 80 amino acids.
We have determined the complete nucleotide sequence of two chicken cDNAs, Ub-t52 and Ub-t80, encoding ubiquitin fused to ribosomal proteins of 52 and 80 amino acids. The deduced amino acid sequences of the ribosomal proteins are identical or very similar to the homologous human and rat proteins and to the corresponding proteins of other species. Unexpectedly, the ubiquitin moiety of the Ub-t52 protein showed two amino acid substitutions: serine-20 has been replaced by asparagine and serine-57 by alanine. Ubiquitin is a protein strongly conserved during evolution, with no changes in sequence previously reported in vertebrates. Ub-t52 and Ub-t80 are highly expressed in early embryogenesis and during postmitotic stages of spermatogenesis, in parallel with the expression of the polyubiquitin gene UbII. Whereas the 5' untranslated regions (5'UTRs) of the chicken polyubiquitin mRNAs showed marked differences in mature testes in relation to somatic tissues, no differences were observed in the 5'UTRs of the ubiquitin-ribosomal protein mRNAs. These mRNAs possess a 5'-terminal oligopyrimidine tract that could be used as a mechanism to postpone translation during postmitotic stages of spermatogenesis, as has been proposed in quiescent cells.
Mezquita
J
J
Molecular Genetics Research Group, Faculty of Medicine, University of Barcelona, Casanova, Spain. mezquita@medicina.ub.es
Pau
M
M
Mezquita
C
C
D000409:Alanine / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine / Q000235:genetics; D001483:Base Sequence; D002642:Chick Embryo / Q000378:metabolism; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D016678:Genome; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011743:Pyrimidines / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000235:genetics; D013091:Spermatogenesis; D014452:Ubiquitins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D011743:Pyrimidines; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D014452:Ubiquitins; D012694:Serine; D001216:Asparagine; D012260:Ribonucleases; D000409:Alanine