Effect of genistein on ATP-induced cell retraction and rounding in human Chang liver cells.
Extracellular ATP has been previously reported to induce cell retraction and stimulate DNA synthesis in human Chang liver cells via phosphoinositide (PI) signal transduction. The ATP-treated cell assumes a rounded morphology and is tethered to the substratum by microvillous anchors as demonstrated in this study by scanning electron and confocal microscopy. The addition of 0.5 mM genistein, which is known to completely abolish extracellular ATP-stimulated DNA synthesis in Chang liver cells, did not inhibit ATP-induced cell rounding. The results suggest that the seemingly conjoint responses of ATP-stimulated DNA synthesis and cell rounding could be dissociated.
Bay
B H
BH
Department of Anatomy, National University of Singapore, Kent Ridge, Singapore.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors
D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D007529:Isoflavones; D000255:Adenosine Triphosphate; D004247:DNA; D019833:Genistein; D011505:Protein-Tyrosine Kinases