Inhibition of IgE production by epsilon (epsilon) chain-specific antisense oligonucleotides (AOs) studied on human myeloma cell line U266 and peripheral blood mononuclear cells of a patient with hypereosinophilia.
Based on cDNA sequence data epsilon chain-specific antisense oligonucleotides were synthesized and checked on in vitro IgE production. Using peripheral blood cells from a hypereosinophilic patient and a human IgE myeloma cell line, U266, marked reduction of in vitro IgE production measured by PRIST was observed. The effect of epsilon antisenses proved to be isotype specific since IgG production by both peripheral blood cells and a lymphoma cell line, CESS, was not affected. Moreover, the expression of other markers on U266 (interleukin-6 receptor and gp130) were not influenced by epsilon-specific antisense oligonucleotides.
Fekete
B
B
Teaching Hospital of Semmelweis University of Medicine, Budapest, Hungary.
Sági
J
J
Szemzõ
A
A
Kovács
L
L
Pálóczi
K
K
Varga
V L
VL
Tamássy
K
K
Falus
A
A
D006801:Humans; D017681:Hypereosinophilic Syndrome / Q000097:blood / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000187:drug effects*; D007139:Immunoglobulin epsilon-Chains / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010954:Plasmacytoma / Q000276:immunology*; D014407:Tumor Cells, Cultured
D007139:Immunoglobulin epsilon-Chains; D016376:Oligonucleotides, Antisense; D007073:Immunoglobulin E