In vivo adenovirus-mediated gene transfer to newborn rat retinal pigment epithelial cells.
A successful surgical access to the subretinal space is critical for achieving adenovirus-mediated gene transfer to the retinal pigment epithelial (RPE) cells or photoreceptor cells. We report a novel surgical approach allowing an efficient delivery of recombinant replication-deficient adenoviral vectors into the subretinal space of newborn rats. Our data suggest that this method may be useful for infecting reproducibly large area of the RPE cell layer of normal newborn rats and should be applicable to RCS pups. We also show the feasibility of infecting ex vivo RPE cells in culture using the same recombinant adenoviral vector.
Ribeaudeau
F
F
Centre de Recherches Thérapeutiques en Ophtalmologie (Certo), Faculté de Médecine Necker, Paris, France.
Abitbol
M
M
Finiels
F
F
Roustan
P
P
Revah
F
F
Dufier
J L
JL
Mallet
J
J
D000818:Animals; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques; D007267:Injections / Q000379:methods; D017921:Mastadenovirus / Q000201:enzymology / Q000235:genetics*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000821:virology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011995:Recombination, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase