Cloning and molecular characterization of an Arabidopsis thaliana RING zinc finger gene expressed preferentially during seed development.
The RING (Really Interesting New Gene) finger is a zinc-binding domain that is found in proteins from a variety of species. This paper reports the cloning and characterization of, as yet, only the second RING finger protein gene from plants, A-RZE, in Arabidopsis thaliana. In addition to the RING-finger motif, A-RZF also contains a putative nuclear localization signal. A-RZF is encoded by a single copy gene with an intron of 595 bp interrupting the 5' leader sequence and the coding region. Northern blot analysis indicated that A-RZF is expressed preferentially during seed development. The RING-finger motif, putative nuclear localization signal, and unique expression pattern, predict an important role during seed development for A-RZF.
Zou
J
J
National Research Council of Canada, Plant Biotechnology Institute, Saskatchewan, Canada.
Taylor
D C
DC
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers / Q000235:genetics*
C108568:A-RZF protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D010940:Plant Proteins