Stability of sulfonamide antibiotics in spiked pig liver tissue during frozen storage.
A bulk portion of homogenized pig liver tissue was spiked at room temperature with 0.2 mg/kg (twice the Australian maximum residue limit) of each of sulfathiazole, sulfachlorpyridazine, sulfadimidine (sulfamethazine), sulfaquinoxaline, and sulfadimethoxine. After subsampling and packaging, selected individual packaged units were tested to confirm homogeneity of the prepared material. The material was stored frozen at -20 degrees C and analyzed in replicate by liquid chromatography on 11 sampling dates over a period of about 6 months. Analytical data were plotted on a log-linear scale and subjected to linear regression on the basis of first-order kinetics for the decay. Storage stabilities (decay half-lives at -20 degrees C) calculated from the mean slope of regression lines were sulfadimethoxine, 567 days; sulfadimidine, 457 days; sulfachlorpyridazine, 312 days; sulfathiazole, 291 days; and sulfaquinoxaline, 271 days. Significant depletion (65% loss) of residue was observed for sulfaquinoxaline during preparation of spiked bulk liver tissue. An extension of the study to measure the storage stability of sulfaquinoxaline under accelerated decay conditions (refrigerator temperature, 4 degrees C) showed it to be relatively unstable, with a decay half-life of 11 days. Results demonstrate the need for both regulatory agencies and testing laboratories to be aware of potential errors associated with improper transport, storage, and handling of tissue samples submitted for antibiotic testing.
Thomas
G K
GK
Australian Government Analytical Laboratories, Cottesloe, Western Australia.
Millar
R G
RG
Anstis
P W
PW
D000704:Analysis of Variance; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D015925:Cryopreservation; D004355:Drug Stability; D006207:Half-Life; D008099:Liver / Q000737:chemistry*; D011786:Quality Control; D013410:Sulfachlorpyridazine / Q000737:chemistry; D013412:Sulfadimethoxine / Q000737:chemistry; D013418:Sulfamethazine / Q000737:chemistry; D013428:Sulfaquinoxaline / Q000737:chemistry; D000077589:Sulfathiazole; D013432:Sulfathiazoles / Q000737:chemistry; D013449:Sulfonamides / Q000737:chemistry*; D013552:Swine
D000890:Anti-Infective Agents; D013432:Sulfathiazoles; D013449:Sulfonamides; D013412:Sulfadimethoxine; D013418:Sulfamethazine; D013410:Sulfachlorpyridazine; D013428:Sulfaquinoxaline; D000077589:Sulfathiazole