Human neutrophils express GH-N gene transcripts and the pituitary transcription factor Pit-1b.
Since GH stimulates the development and function of granulocytes, we investigated the expression of GH in granulocyte subsets. By immunocytochemistry, 25 +/- 7% of the human neutrophils were shown to express immunoreactive GH, whereas eosinophils were negative. Reversed transcription (RT)-PCR analysis demonstrated GH mRNA in neutrophils. Restriction analysis revealed that neutrophils express the GH-N gene but not the GH-V gene. Furthermore, we demonstrated by western blot analysis that neutrophils express an alternatively spliced variant of the pituitary transcription factor Pit-1, designated Pit-1b.
Kooijman
R
R
Department of Pharmacology, Medical School, Free University of Brussels (V.U.B.), Belgium.
Berus
D
D
Malur
A
A
Delhase
M
M
Hooghe-Peters
E L
EL
D000236:Adenoma / Q000737:chemistry / Q000473:pathology; D000328:Adult; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006098:Granulocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009504:Neutrophils / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D010902:Pituitary Gland / Q000737:chemistry* / Q000166:cytology; D010911:Pituitary Neoplasms / Q000737:chemistry / Q000473:pathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; C494187:POU1F1 protein, human; D012333:RNA, Messenger; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; D013006:Growth Hormone