Cloning and expression of cDNA encoding a mitogen-activated protein kinase from a phytopathogenic filamentous fungus.
We have cloned a mitogen-activated protein kinase (MAPK) designated Fusarium solani f. sp. pisi mitogen-activated protein kinase (FsMAPK) from the phytopathogenic filamentous fungus F. solani f. sp. pisi T8 strain. A single open reading frame (ORF) of 1068 bp encoding a polypeptide of 355 amino acids (aa) with a predicted molecular mass of 41,194 Da was found in the cloned 1583-bp cDNA insert. FsMAPK is highly homologous to SPK1 of fission yeast, FUS3 of budding yeast, MsERK1 of alfalfa, Sur-1 of nematode, and hERK1 of human. That this gene is expressed in F. solani f. sp. pisi was shown by the finding that immunoblot of the fungal extracts with anti-FsMAPK antibodies (Ab) raised in a rabbit against the FsMAPK, expressed in Escherichia coli (E. coli), detected the corresponding protein. DNA blot analysis indicated that Fsmapk is present as a single copy in the fungal genome.
Li
D
D
Neurobiotechnology, The Ohio State University, Columbus, 43210, USA.
Rogers
L
L
Kolattukudy
P E
PE
D000595:Amino Acid Sequence; D000908:Antibodies, Fungal / Q000276:immunology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics; D018797:Cell Cycle Proteins; D064447:Checkpoint Kinase 2; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005670:Fusarium / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D005800:Genes, Fungal; D016681:Genome, Fungal; D016695:Glycosyltransferases; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000908:Antibodies, Fungal; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D016695:Glycosyltransferases; C092031:SUR1 protein, S cerevisiae; D011494:Protein Kinases; D064447:Checkpoint Kinase 2; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C075580:FUS3 protein, S cerevisiae; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; C067022:RAD53 protein, S cerevisiae