title: Enterocytic differentiation correlates with changes in the fine structure and sulfation of perlecan in HT29 human colon carcinoma cells.
abstract: Undifferentiated HT29 and differentiated HT29G-human colon carcinoma cells have been used to study the changes in proteoglycan production and structure associated with enterocytic cell differentiation. Differentiated cells incorporate twice as much sulfate than undifferentiated cells when labeled with [35S]sulfate. Both cell lines produce a heparan sulfate proteoglycan which was purified by ion-exchange. The heparan sulfate proteoglycan from differentiated HT29G- cells is larger and more homogeneous in size than that produced by undifferentiated HT29 cells. No differences in the core protein structure were observed. The detailed structural analysis of the heparan sulfate chains revealed that the structure of these chains follows the standard rules for these glycosaminoglycans with N-sulfated domains and N-acetylated domains. The main finding was that differentiated HT29G- cells have a degree of higher sulfation than HT29 cells. These differences were found to affect primarily 6-O-sulfated positions.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Molist
authors.forename: M
authors.initials: M
authors.lastname: Romarís
authors.forename: J
authors.initials: J
authors.lastname: Villena
authors.forename: A
authors.initials: A
authors.lastname: Bassols
mesh_terms: D002277:Carcinoma / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology*; D019073:HT29 Cells; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*
chemical_list: D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D011509:Proteoglycans; D013431:Sulfates; C071927:perlecan