title: The tyrosine phosphatase SHP-1 associates with the sst2 somatostatin receptor and is an essential component of sst2-mediated inhibitory growth signaling.
abstract: Activation of the somatostatin receptor sst2, a member of the Gi protein-coupled receptor family, results in the stimulation of a protein-tyrosine phosphatase activity involved in the sst2-mediated growth inhibitory signal. Here, we report that SHP-1, a cytoplasmic protein-tyrosine phosphatase containing two Src homology 2 domains constitutively associated with sst2 as evidence by coprecipitation of SHP-1 protein with sst2, in Chinese hamster ovary cells coexpressing sst2 and SHP-1. Activation of sst2 by somatostatin resulted in a rapid dissociation of SHP-1 from sst2 accompanied by an increase of SHP-1 activity. SHP-1 was phosphorylated on tyrosine in control cells and somatostatin induced a rapid and transient dephosphorylation on tyrosine residues of the enzyme. Stimulation of SHP-1 activity by somatostatin was abolished by pertussis toxin pretreatment of cells. Gialpha3 was specifically immunoprecipitated by anti-sst2 and anti-SHP-1 antibodies, and somatostatin induced a rapid dissociation of Gialpha3 from sst2, suggesting that Gialpha3 may be involved in the sst2.SHP-1 complexes. Finally, somatostatin inhibited the proliferation of cells coexpressing sst2 and SHP-1, and this effect was suppressed in cells coexpressing sst2 and the catalytic inactive SHP-1 (C453S mutant). Our data identify SHP-1 as the tyrosine phosphatase associated with sst2 and demonstrate that this enzyme may be an initial key transducer of the antimitogenic signaling mediated by sst2.
authors.affiliation: INSERM Unité 151, Institut Louis Bugnard, CHU Rangueil, F 31403 Toulouse Cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Lopez
authors.forename: J P
authors.initials: JP
authors.lastname: Estève
authors.forename: L
authors.initials: L
authors.lastname: Buscail
authors.forename: N
authors.initials: N
authors.lastname: Delesque
authors.forename: N
authors.initials: N
authors.lastname: Saint-Laurent
authors.forename: M
authors.initials: M
authors.lastname: Théveniau
authors.forename: C
authors.initials: C
authors.lastname: Nahmias
authors.forename: N
authors.initials: N
authors.lastname: Vaysse
authors.forename: C
authors.initials: C
authors.lastname: Susini
mesh_terms: D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D006224:Cricetinae; D004789:Enzyme Activation; D047908:Intracellular Signaling Peptides and Proteins; D010766:Phosphorylation; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D017481:Receptors, Somatostatin / Q000378:metabolism*; D015398:Signal Transduction; D013004:Somatostatin / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D017481:Receptors, Somatostatin; D014443:Tyrosine; D013004:Somatostatin; C093733:somatostatin receptor 2; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases