Flow-injection chemiluminescent determination of glycerol-3-phosphate and glycerophosphorylcholine using immobilized enzymes.
Flow injection procedures with immobilized enzyme mini-columns are described for the determination of glycerol-3-phosphate, and glycerophosphorylcholine with chemiluminescent detection. The hydrogen peroxide produced on-line is coupled with a luminol (5-amino-2,3,-dihydro-1,4-phthalazinedione) peroxidation chemiluminescent system. The detection limits for glycerol-3-phosphate and glycerophosphorylcholine are 5 x 10(-7) M and 1 x 10(-6) M respectively with r.s.d. < 2%. The sample throughput is 40/h. The immobilized enzyme columns did not show any deterioration in activity after usage for 3 months.
Yaqoob
M
M
Department of Chemistry, University of Balochistan, Quetta-Pakistan.
Nabi
A
A
Masoom-Yasinzai
M
M
D004800:Enzymes, Immobilized; D004867:Equipment Design; D005993:Glycerolphosphate Dehydrogenase; D005994:Glycerophosphates / Q000032:analysis*; D005997:Glycerylphosphorylcholine / Q000032:analysis*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol; D010739:Phospholipase D; D010783:Photometry / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D005994:Glycerophosphates; D007202:Indicators and Reagents; D008165:Luminol; D005997:Glycerylphosphorylcholine; C029620:alpha-glycerophosphoric acid; D005993:Glycerolphosphate Dehydrogenase; C098831:glycerol-3-phosphate oxidase; D010739:Phospholipase D