HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis.
The protein encoded by the human gene HEC (highly expressed in cancer) contains 642 amino acids and a long series of leucine heptad repeats at its C-terminal region. HEC protein is expressed most abundantly in the S and M phases of rapidly dividing cells but not in terminal differentiated cells. It localizes to the nuclei of interphase cells, and a portion distributes to centromeres during M phase. Inactivation of HEC by microinjection of specific monoclonal antibodies into cells during interphase severely disturbs the subsequent mitoses. Disordered sister chromatid alignment and separation, as well as the formation of nonviable cells with multiple, fragmented micronuclei, are common features observed. These results suggest that the HEC protein may play an important role in chromosome segregation during M phase.
Chen
Y
Y
Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 78245, USA.
Riley
D J
DJ
Chen
P L
PL
Lee
W H
WH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D003598:Cytoskeletal Proteins; D000882:Haplorhini; D006801:Humans; D007668:Kidney / Q000166:cytology; D007930:Leucine; D051379:Mice; D008938:Mitosis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009369:Neoplasms / Q000737:chemistry; D009687:Nuclear Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
D003598:Cytoskeletal Proteins; C120529:NDC80 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D007930:Leucine