title: Birth after combination of cryopreservation of sperm recovered from urine and intracytoplasmic sperm injection in a case of complete retrograde ejaculation.
abstract: OBJECTIVE
  To assess whether sperm recovered from postejaculatory urine and cryopreserved can be used successfully for intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  Case report.
  
  
  METHODS
  Laboratory of Developmental and Reproductive Biology, University Hospital.
  
  
  METHODS
  A couple with male infertility resulting from complete retrograde ejaculation.
  
  
  METHODS
  Freezing of sperm recovered from urine and subsequent use for ICSI.
  
  
  METHODS
  Survival, maintenance of fertilization, and pregnancy potential of sperm recovered from urine and cryopreserved.
  
  
  RESULTS
  On thawing, the sperm were still alive (32% viability) and very slightly motile (2% sluggish motility). Of 16 oocytes injected, 10 (62.5%) produced normal, cleaving embryos. A twin pregnancy with the birth of two healthy infant girls was achieved after the transfer of three embryos.
  
  
  CONCLUSIONS
  The birth of normal, healthy female infants after ICSI with sperm recovered from postejaculatory urine and then cryopreserved demonstrates the usefulness of freezing such sperm even if motility is very low, to avoid surgery in the patient.
authors.affiliation: Centre Hospitalier Universitaire, Hôtel-Dieu, Clermont-Ferrand, France.
authors.forename: C
authors.initials: C
authors.lastname: Jimenez
authors.forename: G
authors.initials: G
authors.lastname: Grizard
authors.forename: J L
authors.initials: JL
authors.lastname: Pouly
authors.forename: D
authors.initials: D
authors.lastname: Boucher
mesh_terms: D000328:Adult; D015925:Cryopreservation; D003593:Cytoplasm; D004542:Ejaculation; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007248:Infertility, Male / Q000628:therapy*; D007267:Injections; D007317:Insemination, Artificial, Homologous; D008297:Male; D011247:Pregnancy; D013094:Spermatozoa