Antioxidant action of estrogens in rat hepatocytes.
The in vitro addition of 17 beta-estradiol (0-100 microM) to isolated rat hepatocytes efficiently prevented cellular lipid oxidation induced by the Fe(III)/ADP complex. 17 beta-estradiol was found to be less effective than its metabolic derivative 2-hydroxyestradiol. The presence of specific inhibitors of cytochrome P450 activity significantly diminished the antioxidant capacity of estradiol. These observations support the hypothesis that estradiol, in the micromolar range, inhibits iron-induced lipid peroxidation in liver cells by diverting reducing equivalents from the peroxidative process to its own metabolism.
Ruiz-Larrea
M B
MB
Departamento de Fisiología, Facultad de Medicina, Universidad del Paás Vasco, Bilboa Spain.
Leal
A M
AM
Martín
C
C
Martínez
R
R
Lacort
M
M
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D065607:Cytochrome P-450 Enzyme Inhibitors; D004958:Estradiol / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000502:physiology; D008099:Liver / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D065607:Cytochrome P-450 Enzyme Inhibitors; D004958:Estradiol