title: Family-10 and family-11 xylanases differ in their capacity to enhance the bleachability of hardwood and softwood paper pulps.
abstract: Enzyme-aided bleaching of softwood and hardwood kraft pulps by glycosyl hydrolase family-10 and -11 xylanases and a family-26 mannanase was investigated. The ability to release reducing sugar from pulp xylan and to enhance bleachability is not a characteristic shared by all xylanases. Of the six enzymes tested, two xylanases belonging to family 11 were most effective at increasing bleachability and improving final paper brightness. None of the enzymes had a deleterious effect on pulp fibre integrity. The efficiency of individual xylanases as bleach enhancers was not dependent on the source microorganism, and could not be predicted solely on the basis of the quantity or nature of products released from pulp xylan. Cooperative interactions between xylanase/xylanase and xylanase/mannanase combinations, during the pretreatment of softwood and hardwood pulps, were investigated. Synergistic effects on reducing-sugar release and kappa number reduction were elicited by a combination of two family-10 xylanases. Pretreatment of kraft pulp with mannanase A from Pseudomonas fluorescens subsp. cellulosa and any one of a number of xylanases resulted in increased release of reducing sugar and a larger reduction in kappa number than obtained with the xylanases alone, confirming the beneficial effects of family-26 mannanases on enzyme-aided bleaching of paper pulp.
authors.affiliation: Department of Cellular Physiology, Babraham Institute, Cambridge, UK.
authors.forename: J H
authors.initials: JH
authors.lastname: Clarke
authors.forename: J E
authors.initials: JE
authors.lastname: Rixon
authors.forename: A
authors.initials: A
authors.lastname: Ciruela
authors.forename: H J
authors.initials: HJ
authors.lastname: Gilbert
authors.forename: G P
authors.initials: GP
authors.lastname: Hazlewood
mesh_terms: D006026:Glycoside Hydrolases / Q000494:pharmacology; D008361:Mannosidases / Q000494:pharmacology; D010209:Paper; D014934:Wood; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000494:pharmacology*; D044902:beta-Mannosidase
chemical_list: D006026:Glycoside Hydrolases; D008361:Mannosidases; D014995:Xylosidases; C023305:hemicellulase; D044902:beta-Mannosidase; D043325:Xylan Endo-1,3-beta-Xylosidase