[Analysis of chemical mediators involved in acute inflammatory reaction with the rat pleurisy model].
Mediators involved in acute inflammation were explored by using rat pleurisies induced by carrageenin or zymosan. The kallikrien-kinin system and eicosanoids were the main mediators responsible for plasma exudation in carrageenin-induced rat pleurisy, while histamine, PAF and the complement system could be the main mediators involved in the vascular permeability increase to cause plasma exudation into the pleural cavity in zymosan-induced rat pleurisy. Several chemokines were detected in the pleural exudates of carrageenin-pleurisy as well as those of zymosan-pleurisy; and exogenous recombinant TNF alpha, IL-1, IL-6 and CINC induced neutrophil migration into rat pleural cavity. These results suggest that these chemokines, directly or indirectly, may partly cause neutrophil migration in the pleural exudates during carrageenin- and zymosan-induced pleurisy. In addition, chemokine production in response to prostanoids and PAF production in response to arachidonic acid were also suggested.
Oh-ishi
S
S
Department of Pharmacology, Kitasato University School of Pharmaceutical Sciences, Tokyo, Japan.
D000818:Animals; D016718:Arachidonic Acid / Q000502:physiology; D002199:Capillary Permeability; D002351:Carrageenan; D002465:Cell Movement / Q000502:physiology; D018925:Chemokines / Q000502:physiology; D003165:Complement System Proteins / Q000032:analysis / Q000502:physiology*; D004195:Disease Models, Animal; D015777:Eicosanoids / Q000502:physiology*; D006632:Histamine / Q000032:analysis / Q000502:physiology*; D007249:Inflammation / Q000139:chemically induced / Q000503:physiopathology*; D016234:Kallikrein-Kinin System / Q000502:physiology*; D008297:Male; D009504:Neutrophils / Q000502:physiology; D010972:Platelet Activating Factor / Q000032:analysis / Q000502:physiology*; D010998:Pleurisy / Q000139:chemically induced / Q000503:physiopathology*; D051381:Rats; D015054:Zymosan
D018925:Chemokines; D015777:Eicosanoids; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D006632:Histamine; D002351:Carrageenan; D003165:Complement System Proteins; D015054:Zymosan