IgM anti-phenolic glycolipid I and IgG anti-10-kDa heat shock protein antibodies in sera and immune complexes isolated from leprosy patients with or without erythema nodosum leprosum and contacts.
The aim of the present work was to evaluate the levels of anti-PGL-I and anti-10-kDa heat shock protein antibodies in serum and immune complexes isolated from leprosy patients, convivients and controls. Leprosy patients with erythema nodosum leprosum or without it were included and a comparative study was done to investigate intergroup differences. Immune complexes were precipitated from serum by polyethylene glycol 3.5%; antibody levels were measured in sera and in dissociated immune complexes by ELISA. Serum antibody levels were then correlated with immune complex-associated antibody levels. The results showed that the erythema nodosum leprosum group differed from controls, contacts and non-erythema patients in their immune complex levels. IgM anti-PGL-I and IgG anti-10-kDa heat shock protein antibodies were constituents of the immune complexes in patients with erythema nodosum leprosum, who exhibited a significant difference in their immune complex composition compared with controls, contacts and non-erythema patients; while free antibody levels (anti-PGL-I and anti-10-kDa) did not differentiate between erythema and non-erythema patients, the measurement of immune complex-associated antibodies demonstrated a significant difference between the two clinical conditions. Furthermore, the measurement of immune complex-associated anti-PGL-I IgM made it possible to differentiate between contacts and controls. The significance of these results is discussed.
Rojas
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Centro de Investigaciones Immunológicas Básicas y Aplicadas (CINIBA), UNLP, La Plata, Argentina.
Demichelis
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Sarno
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EN
Segal-Eiras
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A
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood / Q000276:immunology; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D000942:Antigens, Bacterial / Q000276:immunology; D018835:Chaperonin 10 / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004893:Erythema Nodosum / Q000097:blood / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D015440:Leprosy, Lepromatous / Q000097:blood / Q000276:immunology*; D008875:Middle Aged; D011092:Polyethylene Glycols / Q000494:pharmacology; D011994:Recombinant Proteins / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D018835:Chaperonin 10; D006017:Glycolipids; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins; C045169:phenolic glycolipid I, Mycobacterium leprae; D011092:Polyethylene Glycols