High-level tetracycline resistant Neisseria gonorrhoeae isolated in Portugal.
The first high-level tetracycline resistance (MIC > or = 16 mg/l) isolates of Neisseria gonorrhoeae (TRNG) were reported in 1990 from patients attending a Sexual Transmitted Disease (STD) Center in Lisbon. The TRNG prevalence was 4% in 1991, 5.3% in 1992 and 10,8% in 1994, exploding to 52.2% in 1995. The tet M determinant was evaluated by PCR. The digests of PCRP using HpaII produced the restriction pattern 2 for all the strains, except one (pattern 3). 78.3% of the TRNG strains were beta-lactamase producers and the 4.5 MDa penicillinase plasmid was the dominant (83%), 90% and 93.3% of the TRNG strains belonged to the auxotype NR and to the serogroup IA, respectively. The IA-8/NR class represented 58.3% of the TRNG isolates, suggesting a clonal spreading.
Ferreira
E
E
Sector de Resistência aos Antibióticos, Bacteriologia, Instituto Nacional de Saúde, LISBOA Codex, Portugal.
Louro
D
D
Gomes
J P
JP
Catry
M A
MA
Pato
M V
MV
D000939:Epitopes; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D016133:Polymerase Chain Reaction; D011174:Portugal; D012703:Serotyping; D013753:Tetracycline Resistance; D001618:beta-Lactamases / Q000378:metabolism
D000939:Epitopes; D001618:beta-Lactamases