title: HumCD4--validation of a STR system for forensic purposes in an Austrian Caucasian population sample.
abstract: The short tandem repeat system HumCD4 was amplified by the polymerase chain reaction (PCR) on blood samples from 304 unrelated Austrian Caucasians and analyzed by horizontal, non-denaturing polyacrylamide electrophoresis. The mean exclusion chance was 0.417, the discriminating power 0.850 and the heterozygosity rate 0.628. The observed phenotype distribution is in Hardy-Weinberg equilibrium. In 100 families (200 meioses) no mutations were found. Sufficient amplification could be achieved with as little as 80 pg of high molecular weight cell-line DNA. In a degradation experiment DNA extracted from bloodstains stored for up to 28 days in a moist chamber and DNA boiled for up to 18 min could be amplified. A duplex PCR with TH01 is proposed.
authors.affiliation: Institute for Legal Medicine, University, Graz, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Klintschar
authors.forename: R
authors.initials: R
authors.lastname: Crevenna
mesh_terms: D001317:Austria; D001788:Blood Grouping and Crossmatching; D001801:Blood Stains; D001826:Body Fluids / Q000737:chemistry; D004247:DNA / Q000097:blood*; D005784:Gene Amplification; D005787:Gene Frequency; D006579:Heterozygote; D006801:Humans; D008970:Molecular Weight; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D044465:White People / Q000235:genetics*
chemical_list: D004247:DNA