title: Localization of soluble guanylate cyclase activity in the guinea pig cochlea suggests involvement in regulation of blood flow and supporting cell physiology.
abstract: Although the nitric oxide/cGMP pathway has many important roles in biology, studies of this system in the mammalian cochlea have focused on the first enzyme in the pathway, nitric oxide synthase (NOS). However, characterization of the NO receptor, soluble guanylate cyclase (sGC), is crucial to determine the cells targeted by NO and to develop rational hypotheses of the function of this pathway in auditory processing. In this study we characterized guinea pig cochlear sGC by determining its enzymatic activity and cellular localization. In cytosolic fractions of auditory nerve, lateral wall tissues, and cochlear neuroepithelium, addition of NO donors resulted in three- to 15-fold increases in cGMP formation. NO-stimulated sGC activity was not detected in particulate fractions. We also localized cochlear sGC activity through immunocytochemical detection of NO-stimulated cGMP. sGC activity was detected in Hensen's and Deiters' cells of the organ of Corti, as well as in vascular pericytes surrounding small capillaries in the lateral wall tissues and sensory neuroepithelium. sGC activity was not observed in sensory cells. Using NADPH-diaphorase histochemistry, NOS was localized to pillar cells and nerve fibers underlying hair cells. These results indicate that the NO/cGMP pathway may influence diverse elements of the auditory system, including cochlear blood flow and supporting cell physiology.
authors.affiliation: Department of Biological Chemistry, University of Michigan, Ann Arbor, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Fessenden
authors.forename: J
authors.initials: J
authors.lastname: Schacht
mesh_terms: D000818:Animals; D001011:Aorta / Q000201:enzymology; D002196:Capillaries / Q000737:chemistry / Q000201:enzymology; D003051:Cochlea / Q000098:blood supply / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D003600:Cytosol / Q000737:chemistry / Q000201:enzymology*; D019084:Fluorescent Antibody Technique, Indirect; D006162:Guanylate Cyclase / Q000032:analysis / Q000378:metabolism*; D006168:Guinea Pigs; D006834:Hydrazines / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000378:metabolism; D009589:Nitrogen Oxides; D009599:Nitroprusside / Q000494:pharmacology
chemical_list: D006834:Hydrazines; D009589:Nitrogen Oxides; D009599:Nitroprusside; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D019001:Nitric Oxide Synthase; D006162:Guanylate Cyclase