title: Different bindings of the minor groove ligands DAPI and Hoechst 33258 to multimers of the curved (CA4T4G) and noncurved (CT4A4G) DNA sequences.
abstract: Equilibrium binding properties of DAPI and Hoechst for prototype curved (CA4T4G)n and straight (CT4A4G)n DNAs were derived from fluorescence intensity. Both decamers display a single strong binding site for each ligand. Hoechst binds seven times stronger to curved than to straight DNA. An additional cooperative, much weaker binding site is found for DAPI.
authors.affiliation: Centro di Studio per gli Acidi Nucleici del C.N.R., c/o Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, Rome, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Caneva
authors.forename: A
authors.initials: A
authors.lastname: De Simoni
authors.forename: L
authors.initials: L
authors.lastname: Mayol
authors.forename: L
authors.initials: L
authors.lastname: Rossetti
authors.forename: M
authors.initials: M
authors.lastname: Savino
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D006690:Bisbenzimidazole / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D008024:Ligands; D009841:Oligonucleotides / Q000138:chemical synthesis; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D008024:Ligands; D009841:Oligonucleotides; C007293:DAPI; D004247:DNA; D006690:Bisbenzimidazole