title: Validation and application of a solid phase chemistry method for preparation of high effective specific activity technetium-99m radiopharmaceuticals.
abstract: Solid phase chemistry with UltraLink Iodoacetyl gel was investigated as a method for removing excess ligand present in preparations of diaminedithiol (DADT) 99mTc radiopharmaceuticals. The process was optimized and tested under 99mTc labeling conditions with 2,2,4,9,9-pentamethyl-4,7-diaza-1,10-decanedithiol (N-methyl-DADT), 1, and 4-(1-(2'-methoxyphenyl) piperazinylbutyl)-2,2,9,9-tetramethyl-4,7-diaza-1,10 -decanedithiol (DADT-4C-MPP), 2, a serotonin receptor-binding ligand. The results illustrated that this gel removed > or = 99.9% excess ligand used in labeling reactions, and was comparable to the level of purification usually obtained by the more labor intensive method of HPLC.
authors.affiliation: Department of Environmental Health Sciences, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205-2179, USA.
authors.forename: C H
authors.initials: CH
authors.lastname: Kao
authors.forename: K E
authors.initials: KE
authors.lastname: Baidoo
authors.forename: S Z
authors.initials: SZ
authors.lastname: Lever
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007553:Isotope Labeling; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals / Q000302:isolation & purification*; D011985:Receptors, Serotonin / Q000032:analysis*
chemical_list: D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D011985:Receptors, Serotonin