title: Contributions of the procyclin 3' untranslated region and coding region to the regulation of expression in bloodstream forms of Trypanosoma brucei.
abstract: When bloodstream forms of Trypanosoma brucei differentiate into procyclic forms they rapidly synthesise a new surface coat composed of procyclins. Procyclin genes are transcribed in bloodstream forms at approximately one-tenth of the rate in procyclic forms, but little, if any, mRNA can be detected, indicating that further down-regulation must occur post-transcriptionally. We have examined the role of the 297 bp procyclin 3' untranslated region (UTR) in regulating expression in bloodstream forms and have identified three discrete elements: a dominant, negative element between positions 101 and 173, and two positive elements. When chloramphenicol acetyl transferase (CAT) was used as the reporter gene, deletion of the negative element caused a approximately 6-fold increase in the level of steady state mRNA and > 30-fold increase in CAT activity, suggesting that both RNA stability and translation were affected. Similar results were obtained with glutamic acid/alanine-rich protein (GARP), the T. congolense analogue of procyclin, indicating that the 3' UTR acts independently of the coding region. In contrast, when trypanosomes were stably transformed with a construct in which the procyclin coding region was linked to a truncated form of the 3' UTR which lacked the negative element, they expressed high levels of mRNA, but no protein could be detected in cell lysates or culture supernatants. These results imply that the procyclin coding region exerts yet another layer of control which prevents inappropriate expression of the protein in the mammalian host.
authors.affiliation: Universtität Bern, Inst. für Allgemeine Mikrobiologie, Switzerland.
authors.forename: N
authors.initials: N
authors.lastname: Schürch
authors.forename: A
authors.initials: A
authors.lastname: Furger
authors.forename: U
authors.initials: U
authors.lastname: Kurath
authors.forename: I
authors.initials: I
authors.lastname: Roditi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D017125:Genes, Protozoan; D005809:Genes, Regulator / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000187:drug effects / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D015800:Protozoan Proteins / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017384:Sequence Deletion; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development*; D014353:Trypanosomiasis, African / Q000097:blood* / Q000469:parasitology
chemical_list: C406724:GARP protein, Trypanosoma; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; C053277:procyclic acidic repetitive protein, Trypanosoma; D015500:Chloramphenicol O-Acetyltransferase