title: Alteration in NMDA receptor subunit mRNA expression in vulnerable and resistant regions of in vitro ischemic rat hippocampal slices.
abstract: Brain insults, including cerebral ischemia, can alter glutamate receptor subunit expression in vulnerable neurons. Understanding these post-ischemic changes in glutamate receptors could enhance our ability to identify specific, novel neuroprotective compounds. Reverse transcription-polymerase chain reaction (RT-PCR) amplification was used to quantify the altered expression of the N-methyl-D-aspartate (NMDA) NR2A, NR2B and NR2C subunits relative to one another in rat hippocampal slices in resistant and vulnerable regions following in vitro oxygen-glucose deprivation. Ninety minutes after re-oxygenation and return to 10 mM glucose, there was a significant increase in the expression of NR2C relative to NR2B and NR2A in the slice as a whole, as well as in the selectively vulnerable CA1 region and the resistant CA3 and dentate gyrus regions.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario. dan.small@nrc.ca
authors.forename: D L
authors.initials: DL
authors.lastname: Small
authors.forename: M O
authors.initials: MO
authors.lastname: Poulter
authors.forename: A M
authors.initials: AM
authors.lastname: Buchan
authors.forename: P
authors.initials: P
authors.lastname: Morley
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000098:blood supply* / Q000378:metabolism*; D066298:In Vitro Techniques; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate