title: Phorbol myristate acetate-dependent association of protein kinase C alpha with phospholipase D1 in intact cells.
abstract: A phospholipase D1 (PLD1) was purified from rat brain by the use of antibody-coupled protein A Sepharose. We found that protein kinase C alp (PKCalpha) stimulated PLD1 activity in the presence of phorbol myristate acetate (PMA). PMA-dependent association of PKCalpha with PLD1 was verified in NIH-3T3 fibroblast cells, and COS7 cells transiently expressing PLD1 as well as in vitro suggesting that the activation of PLD1 resulted from direct association of PKCalpha with PLD1.
authors.affiliation: Department of Life Science, Pohang University of Science and Technology, South Korea.
authors.forename: T G
authors.initials: TG
authors.lastname: Lee
authors.forename: J B
authors.initials: JB
authors.lastname: Park
authors.forename: S D
authors.initials: SD
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Hong
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.forename: K S
authors.initials: KS
authors.lastname: Yi
authors.forename: S
authors.initials: S
authors.lastname: Bae
authors.forename: Y A
authors.initials: YA
authors.lastname: Hannun
authors.forename: L M
authors.initials: LM
authors.lastname: Obeid
authors.forename: P G
authors.initials: PG
authors.lastname: Suh
authors.forename: S H
authors.initials: SH
authors.lastname: Ryu
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001921:Brain / Q000201:enzymology; D019556:COS Cells; D004789:Enzyme Activation; D051379:Mice; D010739:Phospholipase D / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D011493:Protein Kinase C; D010739:Phospholipase D; C106801:phospholipase D1; D013755:Tetradecanoylphorbol Acetate