[Various properties of liver transketolase].
Transketolase was isolated from rat liver tissue by ion exchange chromatography on Phosphocellulose. The enzyme was eluted by fructose-6-phosphate. The final product had a specific activity of 2.68 units/mg. The enzyme yield is 65% of its total content in the original extract. The possibility of regulation of transketolase activity by keto- and aldo-substrates has been studied; their interrelations are determined by the competition for relations with free and substituted forms of the enzyme.
Kubyshin
V L
VL
Gorbach
Z V
ZV
D000818:Animals; D001667:Binding, Competitive; D005636:Fructosephosphates / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010428:Pentosephosphates / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity; D014174:Transketolase / Q000378:metabolism*
D005636:Fructosephosphates; D010428:Pentosephosphates; C027618:fructose-6-phosphate; D014174:Transketolase