Rapid detection of Brucella melitensis from blood cultures by a commercial system.
To assess the capability of the Peds Plus medium of the Bactec 9240 blood culture system to recover Brucella melitensis within the routine seven-day protocol used by most clinical microbiology laboratories, inoculated blood culture bottles were monitored by the Bactec 9240 instrument for four weeks, and blind subcultures were performed once a week. A total of 2579 blood cultures were drawn, 42 (1.6%) of which were positive for Brucella melitensis. Forty-one of the 42 (97.6%) positive cultures were detected by the Bactec 9240 instrument within two to six days; a single positive culture was missed by the instrument and detected by blind subculture performed on day 7.
Yagupsky
P
P
Clinical Microbiology Laboratory, Saroka Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Peled
N
N
Press
J
J
Abu-Rashid
M
M
Abramson
O
O
D000293:Adolescent; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D017347:Brucella melitensis / Q000302:isolation & purification*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D003937:Diagnosis, Differential; D005334:Fever / Q000097:blood* / Q000209:etiology; D006801:Humans; D011446:Prospective Studies; D012680:Sensitivity and Specificity
D003470:Culture Media