Survival of a bacterioferritin deletion mutant of Brucella melitensis 16M in human monocyte-derived macrophages.
A bacterioferritin (BFR) deletion mutant of Brucella melitensis 16M was generated by gene replacement. The deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pBBR-bfr. The survival and growth of the mutant, B. melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not essential for the intracellular survival of B. melitensis in human MDM.
Denoel
P A
PA
U. R. Biologie Moléculaire, Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre Dame de la Paix, Namur, Belgium. Philippe.Denoel@fundp.ac.be
Crawford
R M
RM
Zygmunt
M S
MS
Tibor
A
A
Weynants
V E
VE
Godfroid
F
F
Hoover
D L
DL
Letesson
J J
JJ
D001426:Bacterial Proteins; D017347:Brucella melitensis / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D003573:Cytochrome b Group / Q000235:genetics*; D005293:Ferritins / Q000235:genetics*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology; D009154:Mutation; D018384:Oxidative Stress; D013045:Species Specificity
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D005293:Ferritins; C018032:bacterioferritin