Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I.
The Corynebacterium glutamicum glutamine synthetase I (GSI) structural gene glnA was cloned by a PCR approach using oligonucleotide primers derived from conserved amino acid sequences of the GSI proteins from various bacteria. Disruption or deletion of this gene in C. glutamicum led to a glutamine auxotrophic phenotype and complete loss of glutamine synthetase activity, indicating the key role of this enzyme in nitrogen metabolism. Additionally, indications for a second glutamine synthetase, GSII, were found.
Jakoby
M
M
Forschungszentrum Jülich, Institut für Biotechnologie I, Germany.
Tesch
M
M
Sahm
H
H
Krämer
R
R
Burkovski
A
A
D000596:Amino Acids / Q000378:metabolism; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000201:enzymology* / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D017353:Gene Deletion; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009584:Nitrogen / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000596:Amino Acids; D004269:DNA, Bacterial; C083818:glutamine synthetase 2; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen