Self-fertility and uni-directional mating-type switching in Ceratocystis coerulescens, a filamentous ascomycete.
Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demonstrate that this species has two mating-types. Only MAT-2 strains are capable of selfing, and half of the progeny from a MAT-2 selfing are MAT-1. Male-only, MAT-2 mutants are self-sterile and cross only with MAT-1 strains. Similarly, self-fertile strains generally cross with only MAT-1 strains. MAT-1 strains only cross with MAT-2 strains and never self. It is hypothesized that the switch in mating-type during selfing is associated with a deletion of the MAT-2 gene.
Harrington
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TC
Department of Plant Pathology, Iowa State University, Ames 50011, USA.
McNew
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DL
D001203:Ascomycota / Q000187:drug effects / Q000502:physiology*; D003433:Crosses, Genetic; D003513:Cycloheximide / Q000494:pharmacology; D012734:Disorders of Sex Development; D004352:Drug Resistance, Microbial; D010641:Phenotype; D012098:Reproduction; D013172:Spores, Fungal
D003513:Cycloheximide