A simple PCR-based procedure for plague diagnosis.
Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals.
Leal
N C
NC
Centro de Pesquisas Aggeu Magalhaes, FIOCRUZ/MS, Cidade Universitária, Recife, PE, Brazil.
Abath
F G
FG
Alves
L C
LC
de Almeida
A M
AM
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D051379:Mice; D010930:Plague / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000235:genetics*
D004269:DNA, Bacterial