title: Rig2, a RING finger protein that interacts with the Kin28/Ccl1 CTD kinase in yeast.
abstract: Kin28/Cell, a cyclin-dependent kinase, is essential for the in vivo phosphorylation of the C-terminal domain of the largest subunit of RNA polymerase II in Saccharomyces cerevisiae. In a search for mutations co-lethal with a thermosensitive kin28 mutation, we have identified genes whose products interact functionally with Kin28. In the present work, we have studied a new complementation group of synthetic lethal mutations. The corresponding gene, RIG2, encodes a predicted RING finger protein. Rig2 is likely to be a homolog of MAT1 of higher eukaryotes which forms a ternary complex with MO15(cdk7) and cyclin H. Our genetic data suggest that Rig2 is a component of transcription factor TFIIH. Transcription activity in a rig2-ts mutant is impeded at restrictive temperature. However, none of the rig2-ts mutants obtained was UV sensitive, suggesting that Rig2 is dispensable for nucleotide excision repair.
authors.affiliation: Institut Curie - Section Recherche, UMR 216-CNRS, Orsay, France. faye@curie.u-psud.fr
authors.forename: G
authors.initials: G
authors.lastname: Faye
authors.forename: M
authors.initials: M
authors.lastname: Simon
authors.forename: J G
authors.initials: JG
authors.lastname: Valay
authors.forename: D
authors.initials: D
authors.lastname: Fesquet
authors.forename: C
authors.initials: C
authors.lastname: Facca
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases; D017931:DNA Primers / Q000235:genetics; D004260:DNA Repair; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D019473:Transcription Factors, TFII; D014466:Ultraviolet Rays; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D029701:Saccharomyces cerevisiae Proteins; C490039:TAF6 protein, S cerevisiae; D035381:TATA-Binding Protein Associated Factors; C108124:TFB3 protein, S cerevisiae; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH; D011494:Protein Kinases; C060137:carboxy-terminal domain kinase; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; C084210:Kin28 protein kinase, S cerevisiae; D012319:RNA Polymerase II