Glycine facilitates gamma-glutamylcysteinylethyl ester-mediated increase in liver glutathione level.
gamma-Glutamylcysteinylethyl ester (gamma-GCE) increases reduced glutathione (GSH) levels in GSH-depleted rat hepatocytes. Because glycine, a constituent of GSH, exists at 0.3 to 0.4 mM in rat plasma, we examined the influence of glycine added to the medium on the action of gamma-GCE to increase GSH levels in the rat hepatocytes. Glycine (0.2-0.8 mM) dose-dependently enhanced gamma-GCE-mediated increase in intracellular GSH levels with an increase in intracellular gamma-GCE levels. These results indicate that exogenous glycine facilitates gamma-GCE-mediated increase in intracellular GSH levels in rat hepatocytes possibly by enhancing the uptake of gamma-GCE into the cells.
Nishida
K
K
Department of Biochemistry, School of Medicine, Fujita Health University, Aichi, Japan. knishida@fujita-hu.ac.jp
Ohta
Y
Y
Ishiguro
I
I
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D010545:Peroxides; D051381:Rats; D017382:Reactive Oxygen Species; D020122:tert-Butylhydroperoxide
D010545:Peroxides; D017382:Reactive Oxygen Species; C042431:S-ethyl glutathione; D020122:tert-Butylhydroperoxide; D005978:Glutathione; D005998:Glycine