[Distribution of amyloid precursor protein mRNA in Alzheimer brain tissues].
This study aimed to provide clear data on the localization of the mRNA for the amyloid precursor protein (APP) in human brain. It is known to occur in neurons but the possible localization in other type of brain cells is controversial among several authors. A cDNA probe constructed by polymerase chain reaction for APP, which was not specific for a particular isoform, gave punctate staining and showed a clear localization not only in neurons but in astrocytes in all brains, as well as in the reactive microglia associated with senile plaques in Alzheimer disease. A cRNA probe gave more diffuse staining of neurons and glia.
Mori
M
M
Department of Neurology, School of Medicine, Chiba University, Japan.
Yoshiyama
Y
Y
Waragai
M
M
Yoshikawa
K
K
Yamada
T
T
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D015342:DNA Probes; D006801:Humans; D017403:In Situ Hybridization; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism
D016564:Amyloid beta-Protein Precursor; D015342:DNA Probes; D012333:RNA, Messenger