title: Electrogenic ion transport along the human duodenum in childhood.
abstract: BACKGROUND
  To find reliable sites to study the effects of different secretagogues on electrogenic ion secretion, we investigated the secretion pattern in different parts of duodenum.
  
  
  METHODS
  Histologically normal routine intestinal biopsy specimens from children were mounted in a modified Ussing chamber. The secretory responses to prostaglandin E2 (PGE2), aminophylline, dibutyryl cyclic adenosine 5'-monophosphate (cAMP), and acetylcholine (ACh) were studied with continuous measurements of the potential difference. Tissue resistance and generated current were calculated.
  
  
  RESULTS
  ACh induced secretion in the whole of duodenum, although the secretory response was augmented distally. PGE2 and cAMP induced significant secretion only in the distal duodenum.
  
  
  CONCLUSIONS
  The ACh-induced, calcium-mediated, electrogenic secretion was expressed along the whole duodenum, whereas the cAMP-mediated secretion was only seen in the distal part. The fully expressed electrogenic chloride secretion was only seen at or distal to the duodenojejunal flexure. Our study shows that it is important to carefully define the localization of physiologic studies performed in the duodenum.
authors.affiliation: Dept. of Pediatrics, Faculty of Medicine, Göteborg University, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Reims
authors.forename: S
authors.initials: S
authors.lastname: Redfors
authors.forename: M
authors.initials: M
authors.lastname: Hemlin
authors.forename: A
authors.initials: A
authors.lastname: Mellander
authors.forename: B
authors.initials: B
authors.lastname: Strandvik
mesh_terms: D000109:Acetylcholine / Q000493:pharmacokinetics; D000628:Aminophylline / Q000493:pharmacokinetics; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D000242:Cyclic AMP / Q000493:pharmacokinetics; D015232:Dinoprostone / Q000493:pharmacokinetics; D004386:Duodenum / Q000473:pathology / Q000502:physiology*; D004594:Electrophysiology; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D017136:Ion Transport; D018709:Statistics, Nonparametric
chemical_list: D000628:Aminophylline; D000242:Cyclic AMP; D015232:Dinoprostone; D000109:Acetylcholine