title: Centromeric behaviour in wheat with high and low homoeologous chromosomal pairing.
abstract: Control of homoeologous chromosomal pairing in hexaploid wheat stems from a balance between a number of suppressor and promoter genes. This study used centromeric behaviour as a tool to investigate the mechanism. Fluorescent in situ hybridization employing centromeric and telomeric sequences as probes was applied to pollen mother cells of wheat and wheat/alien hybrids having different pairing gene combinations. It showed: association of centromeres during pre-meiotic interphase; decondensation of centromeric structure; sister chromatid disjunction of univalent chromosomes in homoeologous pairing situations at anaphase I; and centromeric stretching between univalent sister chromatids in wheat/rye hybrids deficient for pairing genes. The implications of these results are discussed.
authors.affiliation: John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.
authors.forename: L
authors.initials: L
authors.lastname: Aragón-Alcaide
authors.forename: S
authors.initials: S
authors.lastname: Reader
authors.forename: T
authors.initials: T
authors.lastname: Miller
authors.forename: G
authors.initials: G
authors.lastname: Moore
mesh_terms: D000705:Anaphase / Q000235:genetics; D002503:Centromere / Q000235:genetics; D002678:Chimera; D002842:Chromatids; D002875:Chromosomes; D015342:DNA Probes; D006570:Heterochromatin / Q000235:genetics; D017403:In Situ Hybridization; D007399:Interphase / Q000235:genetics; D008540:Meiosis; D012434:Secale / Q000235:genetics; D016615:Telomere / Q000235:genetics; D014908:Triticum / Q000235:genetics*
chemical_list: D015342:DNA Probes; D006570:Heterochromatin