Specificities of anti-sialyl-Tn and anti-Tn monoclonal antibodies generated using novel clustered synthetic glycopeptide epitopes.
The fine specificities of MAbs generated using novel synthetic clustered STn and Tn glycopeptides as immunogens were compared with the anti-TAG-72 antibodies B72.3 and CC49. Hapten inhibition experiments demonstrated the specificity of several of the MAbs for STn and Tn expressed on ovine submaxillary mucin and tumor derived MUC-1 mucin. Amongst the STn specific MAbs only the B195.3 MAb shows absolute dependence on the presence of sialic acid and specificity to the simple disaccharide NANAA alpha2-6-GalNAc. Identification of tumor associated carbohydrate epitopes in cluster and monomer configurations are possible using MAbs detecting the defined structure specificities described herein.
Reddish
M A
MA
Biomira Inc., Edmonton, Alberta, Canada. m_Reddish@biomira.com
Jackson
L
L
Koganty
R R
RR
Qiu
D
D
Hong
W
W
Longenecker
B M
BM
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis* / Q000276:immunology; D001202:Ascitic Fluid / Q000737:chemistry; D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005260:Female; D006020:Glycopeptides / Q000032:analysis / Q000138:chemical synthesis; D006031:Glycosylation; D006241:Haptens; D051379:Mice; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000096:biosynthesis / Q000737:chemistry; D009077:Mucins / Q000096:biosynthesis / Q000737:chemistry; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D011863:Radioimmunoassay; D012756:Sheep; D013363:Submandibular Gland / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D000939:Epitopes; D006020:Glycopeptides; D006241:Haptens; D018396:Mucin-1; D009077:Mucins; C040286:Tn antigen; C055491:sialosyl-Tn antigen; D019158:N-Acetylneuraminic Acid