Incorporation of cells into an ELISA system enhances antigen-driven lymphokine detection.
The ability to measure successfully the levels of Th1 or Th2 cytokines during an in vitro antigen-driven, polyclonal T-cell response has proven to be more difficult than expected. Here we describe the development of a highly sensitive cell-based ELISA (celELISA) technique for the detection of murine Th1 and Th2 cytokines. The celELISA combines the quantification aspects of the conventional sandwich ELISA with the sensitivity of the ELISPOT assay. The celELISA was particularly useful for the improved detection of IL-2, IL-4, and to a lessor extent, IFN-gamma.
Beech
J T
JT
Department of Pathology and Microbiology, Medical School, University of Bristol, UK.
Bainbridge
T
T
Thompson
S J
SJ
D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D001426:Bacterial Proteins; D002478:Cells, Cultured; D018834:Chaperonin 60; D018833:Chaperonins / Q000008:administration & dosage / Q000276:immunology; D003208:Concanavalin A / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D007114:Immunization; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D007376:Interleukin-2 / Q000032:analysis / Q000096:biosynthesis; D015847:Interleukin-4 / Q000032:analysis / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000032:analysis* / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D012680:Sensitivity and Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D000941:Antigens; D001426:Bacterial Proteins; D018834:Chaperonin 60; D007376:Interleukin-2; D008222:Lymphokines; C074119:heat-shock protein 65, Mycobacterium; D003208:Concanavalin A; D015847:Interleukin-4; D007371:Interferon-gamma; D018833:Chaperonins