Primary structure of seven sulfated oligosaccharide-alditols released by reductive beta-elimination from oviducal mucins of Rana temporaria. Characterization of the sequence HSO3 (3) GlcA (beta1--3)Gal.
The mucins isolated from Rana temporaria egg jelly coats were found to be composed of Gal, Fuc, GlcNac, GalNAc and GlcA acid. The primary structure of seven sulfated oligosaccharide-alditols was obtained by 1D/2D NMR analyses (1H-13C). The results show the presence of the sulfate monosaccharides. HSO3(3)Gal, HSO(6)GlcNAc and HSO3(3)GlcA. The sequence HSO3(3)GlcA (beta1-3)Gal, which constitutes the major determinant of the HNK-1 oncofoetal epitope, was characterized.
Florea
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Department de Biochimie, Université de Bucarest, Romania.
Maes
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Strecker
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D000818:Animals; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000737:chemistry; D005697:Galactosides / Q000737:chemistry; D005965:Glucuronates / Q000737:chemistry; D020723:Glucuronic Acid; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011896:Rana temporaria; D017421:Sequence Analysis; D013402:Sugar Alcohols / Q000737:chemistry*; D013463:Sulfuric Acid Esters / Q000737:chemistry*; D015044:Zona Pellucida / Q000737:chemistry*
D004187:Disaccharides; D005697:Galactosides; D005965:Glucuronates; D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols; D013463:Sulfuric Acid Esters; D020723:Glucuronic Acid